LOCUS       DAOBBH010000001        49961 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.001, whole genome shotgun sequence.
ACCESSION   DAOBBH010000001 DAOBBH010000000
VERSION     DAOBBH010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 49961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49961
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.001"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..1513
                     /locus_tag="P6L01_000001"
     CDS             <1..1513
                     /locus_tag="P6L01_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029107.1"
                     /translation="LKIIIPIIIVLLLIGGIAWGVYAFFANTPKNTYLKSEQQTAKMY
                     KDYFNDRFENEVKFQEKMKDNSFLSSLELSADASDEIVKGLGIPKSVVNASKIKMSYG
                     HDPKKEKSMINLEPTIADSALGKFQLAADKDKHYFESPLFKGKYSVNNSDLLSTYSKL
                     TGEDEETAKENGITNQQLNLNTLFSNAQAQQSDYSKIAEKYSELIVDKLDDDNFDKGK
                     KEEIKVNGEKYKVRPVTLTLSRADTKKITLAVLEEAKKDKDLKKLMEEQGATKDYDKD
                     IKKAIDDVKETKKDEFAKIQSKIYTEKHTIVKREITITDKENNKTKIKGTNTLEDDKL
                     KLDYALDFDQDKYTYAEAKYTIKGVSSKEKDNKYSDKYEFGKKTEYDESKIKLDNQEK
                     VDGTKRQDKGKITVALDKYSDENEFTFENNIDSDVKNNTQKSTLNIGIKYAEEPINFI
                     LKSSTKLKADIDFDDSGAKDFNSLSSKDREKLEKEIEKNGGKMFESILKKASK"
     gene            1581..2747
                     /locus_tag="P6L01_000002"
     CDS             1581..2747
                     /locus_tag="P6L01_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3029108.1"
                     /translation="MKTIHLFRIYHSFLLKKWYLIIYLLFILAALLITLTTIQHVTED
                     DNHFNIGVVDKDQSSETKLILNSIGKGSNLGKNVSIKAYDDKQAHTLLKKHKLQGYFV
                     FDKGMTKAFYKQGELPISVYTYDQQSMKSVVLSQLTDSVYQRLMRSMGGILAFQDLAP
                     KASHSDSINVMTDLLITGLNRSGAFNLEPIHLYDTGSYYAITGFLATVFIFALSLFTV
                     LKMNQDTVLKARLKMFHFSKERLLIIRALITWFYTMLWSIVGVVWIVFSIPNTFELYN
                     WPTLAIHLSYYVTFLILWLLLIELLTTGLLNSISKVILAIVILVLSGLTIPTIFLQHI
                     ANGVFNIQPFAVVTNQLLEIILNNYILELHPSFYLSFIALLIINLAVLVWRYRQ"
     gene            2744..3664
                     /locus_tag="P6L01_000003"
     CDS             2744..3664
                     /locus_tag="P6L01_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3029109.1"
                     /translation="MKPYIQLVLFKQWLQYILLVTTIVIALVLIGIGYRVAHDNFKIP
                     ITIQDLDQTTASKSFVNKIKQSDYVTIKKVDEDESYIEDDVTKKEAILSMQIPKGFSQ
                     KLKENRLKETIQLYGRDDFIGGIAIEIVSSSLYEQQIPNIIYEHLEDMKQHQSIDAIN
                     KSYHKHTPESKIKFVSLTKQAQHSISISLIFAVILFVSAVQVVLHYRLNQQAALQRLS
                     QYHLSRFKLYSTYVMTHTILLLLVLLAVSLYLSQPLSLIFYLKSLLLILIYEIGIVFI
                     LFHIQTISHRLFMTFIYALAMGIVYLIIFM"
     gene            3678..4337
                     /locus_tag="P6L01_000004"
     CDS             3678..4337
                     /locus_tag="P6L01_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3029110.1"
                     /translation="MIEINNLSKRYRNKQIFNHLTMSFDSNRLTVLLGDNGAGKSTLL
                     RMIAGIEKANDGTINYFGEKWNQRQIQNHIGYVPQDIALFEHMTVAENIKFFKSLCKN
                     PINDTTINEYLQQLNFDDTSAKVSTLSGGNKRKINILVGLLGQPRILILDEPTVGIDL
                     KSRHDIHQLLNIMKSKCLIILTTHHLDEVEALADDIKLIGQDPFYQHVLEDKQWAYTY
                     Y"
     gene            complement(4389..5339)
                     /gene="lytM"
                     /locus_tag="P6L01_000005"
     CDS             complement(4389..5339)
                     /gene="lytM"
                     /locus_tag="P6L01_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-glycine endopeptidase LytM"
                     /protein_id="HDP3029111.1"
                     /translation="MKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTLMSTQSQD
                     VSYGTYYTIDSNGDYHHTPDGNWNQAMFDNKEYSYTFVDAQGHTHYFYNCYPKNANAN
                     GSGQTYVNPATAGDNNDYTASQSQQHINQYGYQSNVGPDASYYSHSNNNQAYNSHDGN
                     GKVNYPNGTSNQNGGSASKATASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMP
                     ENSPVYSLTDGTVVQAGWSNYGGGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGD
                     QIAYSGSTGNSTAPHVHFQRMSGGIGNQYAVEPTSYLQSR"
     gene            complement(5665..6657)
                     /locus_tag="P6L01_000006"
     CDS             complement(5665..6657)
                     /locus_tag="P6L01_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDP3029112.1"
                     /translation="MCTGFTIQTLNNQVLLGRTMDYDYPLDGSPAVTPRNYRWTSRTG
                     TTGQTQYGFIGTGTDMEGFIYGDGVNEHGVAISTQYFRGYSSYGSTHKADAMNITQNE
                     IVTWILGYTTSIEDMKQQASQIHVVAVYLNDIGEVPPLHYHVSDATGHSVEVSFKEGE
                     VVIKDNPIGVLTNHPDLDWHYSNLRQYINISPYPATAKLLEGVTIEPLGNEAGTFGLP
                     GGFTSTERFVRMAFMKANIAQNNDKEMDLMNAFYLLDAVNIPIGIVRPHDADNHYTMY
                     QTVINLTTRTLYIKYYGSNEIVALKLTDDLINRKDMTIFKPEKHITIRKLNDNQ"
     gene            6891..8267
                     /locus_tag="P6L01_000007"
     CDS             6891..8267
                     /locus_tag="P6L01_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDP3029113.1"
                     /translation="MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTS
                     PAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALL
                     IIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAT
                     NFGWQMNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLT
                     QGFEQGWFSTFSFICLSIFIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVL
                     NSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLII
                     GPLFPVIGITIISMTQLSASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVG
                     LATGLYKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYM
                     IIPKTVKS"
     gene            complement(8395..8787)
                     /locus_tag="P6L01_000008"
     CDS             complement(8395..8787)
                     /locus_tag="P6L01_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029114.1"
                     /translation="MQFKLKEEEIISFLELKYPEKEFEYGRLLVGQHKRDDLDVYYFG
                     DTFLMCTIISFKTFEIKETVELSYDAVNRIVLKDGWLFRKMRIETMQKVLKYGTSKLM
                     LTDFQKENYNKYIQGQKQRVIFENGHFV"
     gene            complement(8837..9016)
                     /locus_tag="P6L01_000009"
     CDS             complement(8837..9016)
                     /locus_tag="P6L01_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HDP3029115.1"
                     /translation="MRRENILLRLIPFNDYDNKNFKMFIVMKNTKVIILKNKIFVFEQ
                     SIKKHNEMMYIELCD"
     gene            9167..10165
                     /locus_tag="P6L01_000010"
     CDS             9167..10165
                     /locus_tag="P6L01_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDP3029116.1"
                     /translation="MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSE
                     RLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTAT
                     DLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
                     EYQGGQLAAQHLIELGHNHMIIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTEL
                     SKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAA
                     YVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN"
     gene            10397..11278
                     /gene="rbsU"
                     /locus_tag="P6L01_000011"
     CDS             10397..11278
                     /gene="rbsU"
                     /locus_tag="P6L01_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose transporter RbsU"
                     /protein_id="HDP3029117.1"
                     /translation="MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIF
                     AIILAVVTSSGFPTGTNLLFALLSGAGWGFGQIITFKAFELIGSSRAMPVTTAFQLLG
                     ASLWGVFALGNWPGIGHKIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVVLLLI
                     GEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMKSENPFRNKITWLQII
                     SGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITI
                     IGLVLILVAASVTVFIK"
     gene            11293..11697
                     /gene="rbsD"
                     /locus_tag="P6L01_000012"
     CDS             11293..11697
                     /gene="rbsD"
                     /locus_tag="P6L01_000012"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDP3029118.1"
                     /translation="MKKSAVLNEHISKAIATIGHFDLLTINDAGMPIPNDHRRIDLAV
                     TKNLPRFIDVLATVLEEMEIQKIYLAEEIKEHNPTQLQQIKQLISSEIEIIFIPHEEM
                     KSNLAHPLNKGNIRTGETTPYSNIALESNVTF"
     gene            11725..12639
                     /gene="rbsK"
                     /locus_tag="P6L01_000013"
     CDS             11725..12639
                     /gene="rbsK"
                     /locus_tag="P6L01_000013"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDP3029119.1"
                     /translation="MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGA
                     NQAIATARMQADTTFITKIGTDGVADFILEDFKAAHIDTSYIIKTTEAKTGQAFITVN
                     AEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEVPIPAIISAFEIAKAHGV
                     TTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSLGIKT
                     VLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAID
                     FGNKASSLTVQKHGAQASIPLLEEVNQV"
     gene            complement(12737..12871)
                     /locus_tag="P6L01_000014"
     CDS             complement(12737..12871)
                     /locus_tag="P6L01_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029120.1"
                     /translation="MIIIIINIAYLYAIIWKLKRLQKIVTSFITKHESTSIGLVENYF
                     "
     gene            12890..13651
                     /locus_tag="P6L01_000015"
     CDS             12890..13651
                     /locus_tag="P6L01_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDP3029121.1"
                     /translation="MSKEAGHTFLAKLGKTRLRPGGKEATDWLIQQGAFSQDKQVLEV
                     ACNMCTTSIYLAHTYGCHIQGVDINKKALEKAQENISAAGLESYIQVQQANAVKLPFD
                     DNQFDIVLNEAMLTMLPIAIKEKALREYYRVLKPGGILLTHDIVIVNESHATHVVKSL
                     SAAINVNVSPQTKLGWLDLYHQAGFNHVHYHTGPMSLMTPKGLIYDEGIVGTIKIIKN
                     ALKKENRPMFCKMFKTMTKLRKDMNYITFVAKKED"
     gene            complement(14138..15574)
                     /locus_tag="P6L01_000016"
     CDS             complement(14138..15574)
                     /locus_tag="P6L01_000016"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDP3029122.1"
                     /translation="MTKLPQNFMWGGALAANQFEGGYEKGGKGLSVIDVMTSGAHGKA
                     RQITESIDLNHYYPNHEGIDFYHRYKEDIALFKEMGLKCLRTSIAWTRIFPNGDEDVP
                     NEEGLAFYDRIFDELIAQGIEPVVTLSHFEMPLHLAKHYGGFRNREVVDYFVHFARVV
                     FERYKDKVTYWMTFNEINNQMDTSNPIFLWTNSGVALTENDNPEEVLYQVAHHELLAS
                     ALAVRLGKEINPKFKIGTMISHVPIYPYSCHPKDMMEAQIANRLRFFFPDVQVRGYYP
                     SYAKKMLARKGYDVGWQEGDDSILQQGTVDYIGFSYYMSTAVKHDVDTTVENNIVNGG
                     LNHSVENPHIATSDWGWAIDPDGLRYTLNVLYDRYQLPLFIVENGFGAVDEVVDGHIH
                     DDYRIEYLKAHITAAIEAVDQDGVDLIGYTPWGIIDIVSFTTGEMKKRYGLIYVDRDN
                     DGHGTMERLKKDSFYWYQQVIASNGDKL"
     gene            complement(15590..16381)
                     /locus_tag="P6L01_000017"
     CDS             complement(15590..16381)
                     /locus_tag="P6L01_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDP3029123.1"
                     /translation="MSNKYKEQAQDILTAVGGVENIVDATYDTKCITIHMQHTIPSTA
                     NEVKQIVDVTSVAENDAQLVIKLNGNVDEVYQQLQRLIKNANVEESENTDNINSQDTS
                     YTPQVKVTTPILVKAPIAGRRILLKEVRDSIFREKMVGEGLAIKAHEESKVIAPFNGL
                     ISMIVPTKHAVGIQSEDGVDIVIHIGVNTVDLEGKGFKCFVKQNDHVEAGQTLLQFDQ
                     QYIQQQGYNADVIVVISNSADLGKVELTMNEIITTEDVIFKIFKN"
     gene            16530..17234
                     /locus_tag="P6L01_000018"
     CDS             16530..17234
                     /locus_tag="P6L01_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDP3029124.1"
                     /translation="MLKYEHIAKQLNAFIHQSNFKPGDKLPNVTQLKERYQVSKSTII
                     KALGLLEQDGLIYQAQGSGIYVRNIADANRINVFKTNGFSKSLGEHRMTSKVLVFKEM
                     ATPPKSVQDELQLNADDTVYYLERLRFVDDDVLCIEYSYYHKEIVKYLNDDIAKGSIF
                     DYLESNMKLRIGFSDIFFNVDKLTSSEASLLQLSTGEPCLRYHQTFYTMTGKPFDSSD
                     IVFHYRHAQFYIPSKK"
     gene            complement(17342..18043)
                     /gene="lrgB"
                     /locus_tag="P6L01_000019"
     CDS             complement(17342..18043)
                     /gene="lrgB"
                     /locus_tag="P6L01_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HDP3029125.1"
                     /translation="MINHLALNTPYFGILLSVIPFFLATILFEKTNRFFLFAPLFVSM
                     VFGVAFLYLTGIPYKTYKIGGDIIYFFLEPATICFAIPLYKKREVLVKHWHRIIGGIG
                     IGTVVALLIILTFAKLAQFANDVILSMLPQAATTAIALPVSAGIGGIKELTSLAVILN
                     GVIIYALGNKFLKLFRITNPIARGLALGTSGHTLGVAPAKELGPVEESMASIALVLVG
                     VVVVAVVPVFVAIFF"
     gene            complement(18036..18479)
                     /gene="lrgA"
                     /locus_tag="P6L01_000020"
     CDS             complement(18036..18479)
                     /gene="lrgA"
                     /locus_tag="P6L01_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like murein hydrolase modulator LrgA"
                     /protein_id="HDP3029126.1"
                     /translation="MVVKQQKDASKPAHFFHQVIVIALVLFVSKIIESFMPIPMPASV
                     IGLVLLFVLLCTGAVKLGEVEKVGTTLTNNIGLLFVPAGISVVNSLGVISQAPFLIIG
                     LIIVSTILLLICTGYVTQIIMKVTSRSKGDKVTKKIKIEEAQAHD"
     gene            complement(18592..19332)
                     /locus_tag="P6L01_000021"
     CDS             complement(18592..19332)
                     /locus_tag="P6L01_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LytR"
                     /protein_id="HDP3029127.1"
                     /translation="MKALIIDDEPLARNELTYLLNEIGGFEEINEAENVKETLEALLI
                     NQYDIIFLDVNLMDENGIELGAKIQKMKEPPAIIFATAHDQYAVQAFELNATDYILKP
                     FGQKRIEQAVNKVRATKAKDDNNASAIANDMSANFDQSLPVEIDDKIHMLKQQNIIGI
                     GTHNGITTIHTTNHKYETTEPLNRYEKRLNPTYFIRIHRSYIINTKHIKEVQQWFNYT
                     YMVILTNGVKMQVGRSFMKDFKASIGLL"
     gene            complement(19335..21089)
                     /locus_tag="P6L01_000022"
     CDS             complement(19335..21089)
                     /locus_tag="P6L01_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDP3029128.1"
                     /translation="MLSLTMLLLERVGLIIILAYVLMNIPYFKNLMNRRRTWKARWQL
                     CIIFSLFALMSNLTGIVIDHQHSLSGSVYFRLDDDVSLANTRVLTIGVAGLVGGPFVG
                     LFVGVISGIFRVYMGGADAQVYLISSIFIGIIAGYFGLQAQRRKRYPSIAKSAMIGIV
                     MEMIQMLSILTFSHDKAYAVDLISLIALPMIIVNSVGTAIFMSIIISTLKQEEQMKAV
                     QTHDVLQLMNQTLPYFKEGLNRESAQQIAMIIKNLMKVSAVAITSKNEILSHVGAGSD
                     HHIPTNEILTSLSKDVLKSGKLKEVHTKEEIGCSHPNCPLRAAIVIPLEMHGSIVGTL
                     KMYFTNPNDLTFVERQLAEGLANIFSSQIELGEAETQSKLLKDAEIKSLQAQVSPHFF
                     FNSINTISALVRINSEKARELLLELSYFFRANLQGSKQHTITLDKELSQVRAYLSLEQ
                     ARYPGRFNININVEDKYRDVLVPPFLIQILVENAIKHAFTNRKQGNDIDVSVIKETAT
                     HVRIIVQDNGQGISKDKMHLLGETSVESESGTGSALENLNLRLKGLFGKSAALQFEST
                     SSGTTFWCVLPYERQEEE"
     gene            complement(21334..22008)
                     /gene="scdA"
                     /locus_tag="P6L01_000023"
     CDS             complement(21334..22008)
                     /gene="scdA"
                     /locus_tag="P6L01_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein ScdA"
                     /protein_id="HDP3029129.1"
                     /translation="MINKNDIVADIVIDYPKAADIFRSVGIDFCCGGQVSIEAASLEK
                     KNVDLNELLQRLNDVEQTNTPGSLNPKFLNVSSLIQYIQAAYHEPLREEFKNLTPYVT
                     KLSKVHGPNHPYLVELKETYDTFKSGMLEHMQKEDDVDFPKLIKYEQGEVVNDINTVI
                     DDLVSDHIATGQLLVKMSDLTSSYEPPIEACGTWRLVYQRLKALEVLTHEHVHLENHV
                     LFKKVS"
     gene            complement(22152..23873)
                     /gene="tarS"
                     /locus_tag="P6L01_000024"
     CDS             complement(22152..23873)
                     /gene="tarS"
                     /locus_tag="P6L01_000024"
                     /EC_number="2.4.1.355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(ribitol-phosphate)
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDP3029130.1"
                     /translation="MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCST
                     DQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETL
                     KDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRT
                     ALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVS
                     PEDFYEVMRLIAVEILNADLEEAHKDQILAEFLNRHFSFSRTNGFSLKVKLEEQPQWI
                     NALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGK
                     LNIQFNEGEPYFEGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHS
                     RNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQ
                     RNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISMKLKGKNKII
                     LTGLDRGYVFEEGMASVVLKDDMIMGMLSQTSENEVEILLSKDIKKRDFKNIVKLNTA
                     HMTYSLK"
     gene            complement(23906..25594)
                     /locus_tag="P6L01_000025"
     CDS             complement(23906..25594)
                     /locus_tag="P6L01_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDP3029131.1"
                     /translation="MVKSKIYIDKIYWERVQLFVEGHSENLDLEDSNFVLRNLTETRT
                     MKANDVKIDGNQFVCRFNVAILDNGYYLPEDKYLLVNEQELDYIAQLNPDVINDAYQN
                     LKPEQEEEYNELETQNGKINFLLQTYLKEFRKGGISKKTVYTVTPEISSDVNEFVLDV
                     VVTTPEVKSIYIVRKYKELRKYFRKQSFNTRQFIFKAIFNTTKFFHLKKGNTVLFTSD
                     SRPTMSGNFEYIYNEMLRQNLDKKYDIHTVFKANITDRRGIIDKFRLPYLLGKADYIF
                     VDDFHPLIYTVRFRRSQEVIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYV
                     SSETDIPFYAEAFGIKEKNVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKVIL
                     FAPTFRGSGHGTAHYPFFKIDFERLARYCEKNNAVVLFKMHPFVKNRLNIADKHKQYF
                     VDVSDFREVNDILFITDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEPYE
                     SFVPGKIVQSFDALMDALDNEDYEGEKVIPFLDKHFKYQDGRSSERLVRNLFGS"
     gene            complement(25616..26641)
                     /locus_tag="P6L01_000026"
     CDS             complement(25616..26641)
                     /locus_tag="P6L01_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDP3029132.1"
                     /translation="MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRY
                     YTGSRDENVLSQKLPMSLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENY
                     LKSSYFRSSGHDGFMQDFVLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKS
                     ISNKNTFGIWGDGNLGYITAILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIP
                     EGLTFDHAFECVGGRGSQSAINQMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFQDVVDLYIQYPDIVDKLALLKGQEYEIATINDLTEAFEADLSTSWGK
                     TVLKWIM"
     gene            complement(26634..27350)
                     /locus_tag="P6L01_000027"
     CDS             complement(26634..27350)
                     /locus_tag="P6L01_000027"
                     /EC_number="2.7.7.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribitol-5-phosphate cytidylyltransferase"
                     /protein_id="HDP3029133.1"
                     /translation="MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILIN
                     DFEKIIIATPQQWMTHTKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGIN
                     DDDVIVTHDAVRPFLTHRIIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVR
                     NEMYQGQTPQSFNINLLKESYAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKV
                     TTPYDLKVANAIIRGGIADD"
     gene            complement(27626..28795)
                     /locus_tag="P6L01_000028"
     CDS             complement(27626..28795)
                     /locus_tag="P6L01_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDP3029134.1"
                     /translation="MIKNTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNP
                     KALYEYLTEHSDAQLIWGVKKGYEHIFQQHNVPYVTKFSMKWFLAMPRAKAWMINTRT
                     PDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSP
                     NPYSTSIFQHAFHVSRDKILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMY
                     APTWRDDEAIREGSYQFNVNFDIEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDV
                     SDYEDISDLYLISDALVTDYSSVMFDFGVLKRPQIFYAYDLDKYGDELRGFYMDYKKE
                     LPGPIVENQTALIDALKQIDETANEYIEARTVFYQKFCSLEDGQASQRICQTIFK"
     gene            complement(29223..30917)
                     /locus_tag="P6L01_000029"
     CDS             complement(29223..30917)
                     /locus_tag="P6L01_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDP3029135.1"
                     /translation="MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTET
                     KTLEANEMKIDGNTFICRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAY
                     AALNEEQIEEYNELTTQNGKVNYLLAYDAKVFRKGGVSQHTVYTITPEIASDVNEFVF
                     DIEITLPQEKSGVIATSAHWLHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFT
                     SDSRPNLSGNFKYVYDELLRQKVDFDYDIKTVFKANITDRRKWRDKFRLPYLLGKADY
                     IFVDDFHPLIYTVRFRPSQEIIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKA
                     YVSSETDIPFYAEAFGIREENVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKV
                     ILFAPTFRGNGHGTAHYPFFKIDFERLARYCEKHNAVVLFKMHPFVKNRLNISREHRQ
                     YFIDVSDHREVNDILFVTDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEP
                     YESFVPGKIVQSFDALMDALDTEDYEVEKVVPFLDKHFKYQDGRSSERLVKDLFRR"
     gene            complement(30939..31964)
                     /locus_tag="P6L01_000030"
     CDS             complement(30939..31964)
                     /locus_tag="P6L01_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDP3029136.1"
                     /translation="MINQVYQLVAPRQFDVTYNNVDIYGNHVIVRPLYLSICAADQRY
                     YTGRRDENVLRKKLPMSLVHEAVGEVVFDSKGVFEKGTKVVMVPNTPTEQHHIIAENY
                     LASSYFRSSGYDGFMQDYVVMAHDRIVPLPNDIDLSTISYTELVSVSYHAIQRFERKS
                     IPLKNSFGIWGDGNLGYITAILLRKLYPEAKIYVFGKTDYKLSHFSFVDDIFTVNQIP
                     DDLKIDHAFECVGGKGSQVALQQIVEHISPEGSIALLGVSELPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFEQVVDLYRKYPDIVEKLALLKGHEINVCTMQDIVQAFEMDLSTSWGK
                     TVLKWTI"
     gene            complement(31957..32673)
                     /locus_tag="P6L01_000031"
     CDS             complement(31957..32673)
                     /locus_tag="P6L01_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDP3029137.1"
                     /translation="MIYAGILAGGIGSRMGNVPLPKQFLDIDNKPILIHTIEKFILVS
                     EFNEIIIATPAQWISHTQDILKKYNITDQRVKVVAGGTDRNETIMNIIDHIRNVNGIN
                     NDDVIVTHDAVRPFLTQRIIKENIEVAAKYGAVDTVIEAIDTIVMSKDKQNIHSIPVR
                     NEMYQGQTPQSFNIKLLQDSYRALSSEQKEILSDACKIIVESGHAVKLVRGELYNIKV
                     TTPYDLKVANAIIQGDIADD"
     gene            complement(33201..34243)
                     /locus_tag="P6L01_000032"
                     /pseudo
     CDS             complement(33201..34243)
                     /locus_tag="P6L01_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498814.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
     gene            complement(34267..34413)
                     /locus_tag="P6L01_000033"
     CDS             complement(34267..34413)
                     /locus_tag="P6L01_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029138.1"
                     /translation="MVESMLTFMLGPLRQITDFYMEHLLVSNSIVIAGYFATGIFKKK
                     KVVN"
     gene            complement(34415..35470)
                     /locus_tag="P6L01_000034"
     CDS             complement(34415..35470)
                     /locus_tag="P6L01_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDP3029139.1"
                     /translation="MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIH
                     TYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCKSKDY
                     NLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAHHGVSKIQV
                     NSGDVAVVMGPGPIGLLVAQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQT
                     DLKTYINGITDGYGADVVVECSGAVPAARQGLDIIRKKGFYSQIGIFKDAEIPFDMEK
                     VIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDISKWDEAYQHLKSGEG
                     IKALLKPLDLDENEGVN"
     gene            complement(35488..36747)
                     /locus_tag="P6L01_000035"
     CDS             complement(35488..36747)
                     /locus_tag="P6L01_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDP3029140.1"
                     /translation="MSYFTDFVRGFLDLGATVILPVVIFLLGLFFRQKIGAAFRSGLT
                     IGVAFVGIFLVIDLLVKNLGPAAQAMVKNLGVSLNVIDVGWPATSSIAWASSVAAFII
                     PLGIIVNVVLLVTKVTKTMNVDIWNFWHYTFTAAMVYAVSGSIWQALLAAVIFQVICL
                     KVADWTAPMMSEFFDLPGVSIATGSTISYAPGIYLVKLLQKVPGLNKLDADPETIQKR
                     FGAFGESIFVGLILGLGIGVLAGYKPGDIINLGMSMAAVMVLMPRMVKILMEGLMPVS
                     ESARTWLNKRFGEREIYIGLDAAVALGHPAVISTALILVPITVLLAVILPGNQVLPFG
                     DLATIPFVVAFIVGAARGNIIHSVIVGTIMIAISLYIATDVAPIFTDMAKGTNVQMPK
                     GSSEISSIDQGGNIVNYLIFKLFSLFN"
     gene            complement(36974..37252)
                     /locus_tag="P6L01_000036"
     CDS             complement(36974..37252)
                     /locus_tag="P6L01_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDP3029141.1"
                     /translation="MKQVLVACGAGIATSTVVNNAIEEMAKEHNIKVDIKQIKITEVG
                     PYEDTADLLVTTAMTKKEYKFPVINARNFLTGIGIEETKQQILTELQK"
     gene            complement(37275..37742)
                     /locus_tag="P6L01_000037"
     CDS             complement(37275..37742)
                     /locus_tag="P6L01_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDP3029142.1"
                     /translation="MGQQLVHKENIMLNLSATDKESVLSQMSDVLFQNGFVKSTFKDA
                     VIDREKEFATGLPTHLCSVAIPHTDVEHINHRTIGVAVLEKEVPFIEMGTLDQQTEVK
                     IVFMLAMDKVDDQLKLLQQLMQIFQSEEKLEQILRTKDETILATLINDYLEYN"
     gene            complement(37727..39823)
                     /locus_tag="P6L01_000038"
     CDS             complement(37727..39823)
                     /locus_tag="P6L01_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDP3029143.1"
                     /translation="MNGDNQQILREIVLNPTIHGKELESIFGLSRRQLGYRIQKINLW
                     LEQEGYPKLERTSQGNFIVSSEIMTLFKRDVSEQQMLNGNNVIFSIETRRYYLMLMLF
                     SKENAMSLNHFSIDLQVSKNTVIHDINHVKEQLEDHGLSLKYSRKHGYEIVGDEFEVR
                     RFFIKLIDQRLNHDITKSEVLKALNLTFEDIAYQKDKIKQVEQFLKSRFIDKSLSSLP
                     YVLCVIRRRIQSGHVMNPLNINYQYLRDTKEYQATEIMTQHEPDLPEAEKLYLTLHLL
                     STSVQWTDLQESDNISNLTMAIAQMIHHFEQITFINIEDKEKLSQQLLLHLTPAFYRI
                     KYNLTDRDELINPLQGNYQSLFHMVKQSCQSLTEYFGKSLPDNEIAYLTMLFGGSLRR
                     QDENFDGKIKAIIVCTQGTSVSQMMLYELRNLFPEIIFLDAISLRTFENYTLDYDIVL
                     SPMFVLTHKKLFITKVALSENEQRKLRKEVMKYINKESADIDKEINKLMALIERTTTV
                     NDITELRDGLEDFIANYNSISTINGSIVTQNKTLDLADLIPARHVKRMHHVENIDEAI
                     AKASDVLVANHFIDIKYIHEMQQVFDDSYMVIMQNIAIPHAYSEKHVHKTAMSMLILQ
                     EPIYMSDGTAIHIIVPIAAVDKVTHLRALLQLRDVAQDNDAIKRIIQSRKNSDVNEIL
                     KNYSNKEARENGWDSN"
     gene            complement(39954..40051)
                     /locus_tag="P6L01_000039"
                     /pseudo
     CDS             complement(39954..40051)
                     /locus_tag="P6L01_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498807.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(40161..41096)
                     /locus_tag="P6L01_000040"
     CDS             complement(40161..41096)
                     /locus_tag="P6L01_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDP3029144.1"
                     /translation="MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVD
                     KNTTNALNVLDIMGRQDIAVAKGADRPLIKPAAFASEIHGESGLDGPKLPSTPSRQAV
                     AMPASDVIINKVMTSDTPVTIVATGPLTKVATALIREPRIAEHIESITLMGGGTFGNW
                     TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADNHVIERFESINNPVAQFVV
                     ELLQFFKKTYKTHFNMDGGPIHDACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAV
                     DLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE"
     gene            41458..42987
                     /locus_tag="P6L01_000041"
     CDS             41458..42987
                     /locus_tag="P6L01_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDP3029145.1"
                     /translation="MKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAY
                     PILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMN
                     ATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVL
                     PPYLGFFGGSRFVPIVTAFAAIFLGVLMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYG
                     FILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGV
                     SRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSF
                     LFVAPILYVIHAFFDGLAFMMADIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVI
                     PIGIVWFCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGKDNIEI
                     VDCCATRLRVTLNQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLG
                     D"
     gene            complement(43307..44260)
                     /locus_tag="P6L01_000042"
     CDS             complement(43307..44260)
                     /locus_tag="P6L01_000042"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDP3029146.1"
                     /translation="MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKV
                     RGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKI
                     FKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
                     SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQT
                     RDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDAVLTVSALLEGEYDEEDVYIGVPA
                     VINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK"
     gene            complement(44571..44678)
                     /locus_tag="P6L01_000043"
     CDS             complement(44571..44678)
                     /locus_tag="P6L01_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029147.1"
                     /translation="MSVCTPTIARIYLTNSKNIVVFLEKSKHDNKNVKV"
     gene            44833..45978
                     /locus_tag="P6L01_000044"
     CDS             44833..45978
                     /locus_tag="P6L01_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide dioxygenase"
                     /protein_id="HDP3029148.1"
                     /translation="MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFN
                     QTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGENL
                     LKAIQDVTGLEEHDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQE
                     SEDIKSFTVETEEYDFSEFTPGQYITVDVSSDKLPYRAKRHYSIVSGEKNHLTFGVKR
                     DVTTEHEGEVSTILHDEIKEGDMINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMY
                     EAASAKGLDTQMVQVAENEQHLPFKDNFNSIASHYDNAKLYTHLKDKQGYIGTEELQA
                     FLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVAV"
     gene            46004..46177
                     /locus_tag="P6L01_000045"
     CDS             46004..46177
                     /locus_tag="P6L01_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029149.1"
                     /translation="MKLNQRYVKVFALYFVSIVTANIIVKNNNLIKTLIQTIAGYTVF
                     AVGLKYLTKRKNK"
     gene            46334..46690
                     /locus_tag="P6L01_000046"
     CDS             46334..46690
                     /locus_tag="P6L01_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDP3029150.1"
                     /translation="MTVDIGRIYDNKDNTDAIRILVDRVWPRGISKRTANLDYWLKDI
                     APSTELRQWFQHDPKLFGAFKEKYEKELRDQDAQKDAFEKLKDIVNQHNHVLLLYAAK
                     DTKHNQAVVLQQLLNT"
     gene            46888..48363
                     /locus_tag="P6L01_000047"
     CDS             46888..48363
                     /locus_tag="P6L01_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDP3029151.1"
                     /translation="MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYE
                     TDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEK
                     VKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYYTDA
                     KSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNL
                     ESQKDDLITDVPVNKVHDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHI
                     IDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKTLLAKDGYTKEHPLK
                     IKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTI
                     PRGDTGYFFNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSR
                     DVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER"
     gene            complement(48677..49819)
                     /locus_tag="P6L01_000048"
     CDS             complement(48677..49819)
                     /locus_tag="P6L01_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HDP3029152.1"
                     /translation="MSSASTQSTKTSDIHNESINKQMEAKAHETAQNADLKTEARSLF
                     DNATKSIGRLAGNDESLNLNLKDMFSEVFKPHTKNEADEIFIAGTAKTTPAICDISEE
                     WGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNA
                     FKNISIFEVIIMFFIGGVFSLLSTMVLYRFVVFSDQFERFGSLTFFDAFLVGLVEETG
                     KALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTR
                     AWTAIGGHLVWSAIVGAAIVIAKEQHGFEFKDIFDKRFLIFFLSAVGLHGIWDTSLTV
                     LGSDTLKIFILIVIVWILVFILMGAGLKQVNLLQKEFKEQQKKVDE"
BASE COUNT        16098 a         9256 c         7603 g        17004 t
ORIGIN      
        1 gttaaaaatt ataattccta ttattattgt cttattatta ataggtggaa tcgcatgggg
       61 agtttatgca ttttttgcaa acacaccgaa aaatacatac ttaaaaagcg aacaacaaac
      121 tgcaaaaatg tataaagatt attttaatga ccgttttgaa aatgaagtga agttccaaga
      181 aaagatgaaa gataattcgt ttttatcttc attagaatta agcgcagatg catcagatga
      241 aattgttaag gggcttggta ttcctaaatc tgttgttaat gcttcgaaaa ttaaaatgtc
      301 atatggacat gatcctaaaa aagagaaatc aatgattaat cttgaaccaa caatcgcaga
      361 ctctgcatta gggaaattcc agttagctgc agataaagat aagcattatt tcgaatcacc
      421 attatttaaa gggaaatata gtgttaataa ttctgattta ttatcaacgt attcaaaact
      481 tacaggtgaa gatgaagaaa cagcaaaaga aaatggtatt acaaaccaac aactaaattt
      541 aaatactctt tttagtaatg ctcaagcaca acaaagtgac tacagcaaaa ttgccgaaaa
      601 atattccgaa cttattgtcg acaaattaga tgacgataat tttgataaag gtaaaaaaga
      661 agaaattaag gttaatggtg aaaagtacaa agttagacct gtcacgttaa cacttagcag
      721 agctgacact aaaaaaatta cattagctgt attagaagaa gctaaaaagg ataaagacct
      781 taaaaaattg atggaagaac aaggtgctac aaaagactat gataaggaca tcaaaaaagc
      841 aattgacgat gtcaaagaaa ctaaaaagga tgaatttgct aaaattcaat ctaaaattta
      901 taccgaaaaa catacgattg taaaacgaga aattactatt acagacaaag aaaataataa
      961 aactaaaatc aaaggtacta atactttaga agacgataag ttaaaactag attacgcact
     1021 tgatttcgat caagataaat acacgtatgc tgaagcgaaa tatacaatta aaggcgtatc
     1081 ttctaaggaa aaagacaata aatacagcga taaatacgaa tttggtaaaa agacagaata
     1141 tgatgaatca aaaatcaaat tagataacca agaaaaagta gatggcacaa aacgtcaaga
     1201 taaaggtaaa atcactgtcg cattagataa atatagcgac gaaaatgaat tcacttttga
     1261 aaataatata gattctgacg taaaaaataa cactcagaaa tctacgttaa atatcggcat
     1321 caaatatgct gaagaaccaa ttaatttcat tttaaaatct agcacaaaat tgaaagcaga
     1381 tattgatttt gatgatagtg gtgcgaaaga tttcaatagt ctatcttcaa aagaccgtga
     1441 aaaacttgaa aaagaaatcg aaaaaaatgg cggcaaaatg tttgaatcaa ttttaaaaaa
     1501 ggcatctaaa taactaaaat ataaaaataa gattgaaagg tcatcttgcc tttcaatctt
     1561 atcattggag gagtggttaa atgaaaacaa tacatttgtt tcgcatctac cactcttttt
     1621 tattgaaaaa gtggtatttg attatctatt tactatttat cttagcggca ctccttatta
     1681 cattaacgac gatccaacat gtaacagaag atgacaatca ttttaatata ggtgtcgtag
     1741 ataaagatca atcaagtgaa acgaaattaa tcttaaactc tattggtaaa gggagtaacc
     1801 taggaaaaaa tgtaagcatt aaagcatatg atgataagca agcacatact ttgttaaaaa
     1861 aacataaact tcaaggctat tttgtttttg ataaaggtat gaccaaggca ttttataaac
     1921 aaggcgaact accaatttca gtatatacat atgatcaaca atccatgaaa agtgtcgtgc
     1981 tatctcagct aacagattct gtttaccaac gtcttatgcg atcaatgggt ggcatcttag
     2041 cttttcaaga cttagcaccg aaagcatcac attctgacag tatcaatgtt atgactgatt
     2101 tgctgattac aggattaaac cgttcaggtg catttaactt agaaccgatt catttatacg
     2161 atacgggcag ttattatgca attacaggat ttttagcaac ggtattcatc tttgcactat
     2221 ctttatttac agttttgaaa atgaatcaag atactgtatt gaaagcgcga ttgaaaatgt
     2281 ttcatttttc taaagagcgt ttattaatca ttcgtgcgtt gattacatgg ttttatacta
     2341 tgttatggag tatcgttggt gtagtttgga ttgtgttcag tattccgaat acctttgaat
     2401 tatataattg gccaacgtta gccattcatt taagctatta tgttaccttt ttaatactgt
     2461 ggttattact catcgaactt ttaacaacag gcttgttgaa tagtattagt aaagtcatat
     2521 tagctattgt catcctggtg ttatctggtt taacaatacc tacgattttt ttacaacata
     2581 tagcaaatgg cgttttcaat attcaaccgt tcgcagttgt aacaaatcaa ttgttagaaa
     2641 ttattttaaa caactacatt ttagaactgc accctagttt ctatcttagc ttcatcgcac
     2701 ttttgataat taatttagct gtattggttt ggaggtatcg tcaatgaagc cttatataca
     2761 acttgttttg ttcaagcaat ggttacaata catcttgctc gtaacaacca ttgtcatcgc
     2821 actcgtactt attggtatcg gttaccgtgt agcacatgac aacttcaaaa taccgattac
     2881 cattcaagat ttagaccaaa ccactgcatc aaaatcattc gtcaataaaa ttaaacaatc
     2941 tgactatgta actattaaaa aagtcgatga agatgaaagc tatattgaag atgatgttac
     3001 taaaaaggaa gctattttaa gcatgcaaat tcctaaaggt ttctctcaaa aattaaaaga
     3061 gaaccgttta aaagaaacga tacagttata tggtagagat gactttatag gtggtattgc
     3121 tatagaaatt gttagtagtt cattatatga acagcaaatt cctaacatta tttatgaaca
     3181 ccttgaggat atgaaacagc atcaatccat cgatgctatc aacaagtcct atcataaaca
     3241 tacacctgaa tctaaaatca aatttgtgtc gcttactaaa caagcacaac actctatttc
     3301 aattagctta atctttgcgg tgattttatt tgttagcgct gttcaagtag tacttcatta
     3361 tcgtttaaac caacaagcag cattgcaacg attatcacaa tatcatttaa gccgtttcaa
     3421 actatatagt acttatgtaa tgacacatac gatcttgtta ttgttggtac tattggcagt
     3481 tagtctatat ttgtctcaac cactcagctt aatattttac ttaaaatcat tgttacttat
     3541 attgatttat gagataggta tcgtctttat cttattccat attcaaacaa taagtcatcg
     3601 tttattcatg acatttatat atgcacttgc tatgggtatc gtatacttga ttattttcat
     3661 gtaaaggagc gtaactgatg atagaaatta ataatctttc aaagcgttac cgtaacaaac
     3721 agattttcaa tcatttaact atgtcctttg atagtaatcg tttaaccgta ttacttggtg
     3781 ataatggtgc tggaaaatca acattacttc gtatgattgc tggtattgaa aaagctaatg
     3841 atggaactat caactatttc ggcgaaaaat ggaatcaaag acaaatacaa aatcacatcg
     3901 gttatgtgcc acaagacatt gcgttatttg aacacatgac agtggctgaa aacattaaat
     3961 tttttaaatc actttgtaaa aatccaatta atgatacaac tatcaacgaa tatttacagc
     4021 aattaaactt tgatgatacg tctgccaaag tatctacatt gtccggtgga aataaacgta
     4081 aaattaatat attagtaggt ttactaggtc aacctcgaat tctcatttta gatgaaccga
     4141 cagttggtat tgatttaaaa tctagacatg acatccacca actacttaac atcatgaaat
     4201 ctaaatgttt aattatatta actacccatc atttagatga agtggaagca cttgcagatg
     4261 atatcaagtt aattggccaa gatccttttt atcaacatgt tttagaggac aagcaatggg
     4321 cttataccta ttattaaaac gaaaaaatcc caagttgcgt atgatattgt aacttgggat
     4381 ttactgtatt atctactttg caagtatgac gttggctcta ctgcatattg attaccgatg
     4441 ccaccagaca tacgttggaa gtgtacgtga ggcgctgttg aattacccgt actacctgaa
     4501 tatgcaattt ggtcaccagc tttgacttta tcaccagctg aaacagttaa acgattatta
     4561 tgcatatacc attggtagtt attactgttc gcttctttaa tagttacttg attgccgcca
     4621 ccatagttac tccaacctgc ttgtactact gtaccatcag ttaatgagta aactggtgaa
     4681 ttttcaggca ttgcatagtc gacaccgtaa tgcgcaccac caccgtgata ttgtccatat
     4741 ggttgtagtt gtttacgact tgttaaccag cttgcgtctt tcgcatgacc actagctgtc
     4801 gctttacttg ctgatccacc attttgatta gatgtgccat taggataatt gacctttcca
     4861 ttaccatcat ggctgttata cgcttggttg ttgttactat gtgaataata gctcgcgtct
     4921 ggacctacat ttgattgata accatattga ttaatatgct gttggctttg actcgctgtg
     4981 tagtcattgt tatctcctgc tgttgctgga ttcacatatg tttggccgct tccattggca
     5041 tttgcatttt ttggataaca gttataaaaa taatgcgtat gtccttgagc atctacgaat
     5101 gtatagctat attctttatt atcaaacatt gcttgattcc agttaccatc aggtgtgtga
     5161 tgataatccc cattagaatc aattgtataa taagtaccat aagatacgtc ttgtgattgt
     5221 gttgacatta gtgtatgtgc ttgttgggtg tttgtcgttt ctgctgcatc tgcttgatgc
     5281 gccattgtaa atgtagcgaa gcccatcgtt gcaatcgctg ctgctgttaa ttttttcatg
     5341 tataaaacat cctccattaa agttaaagtt agttttcaat taaactgtac tgcacatact
     5401 aaaagaatta gacaactgag taaaggattt aattctcatt ttccaactat ttaatattcc
     5461 cgaaatgttt tactaaactc attacattgt cattacaaaa tagccataca ttgatattaa
     5521 aatgacatct ctcactgcat tcgcttaagc ttttataaat tttcaaaatt aacaactaat
     5581 cattcgtcca tgttcgcggt tcaacgctaa tgtatgatag taatcatcca tcaaatcata
     5641 tcaaccaaat tccattctca atcgctattg attgtcattc aactttctaa tagtgatatg
     5701 cttctcaggc ttaaaaatcg tcatatcttt tctattaatt aaatcatctg tgagctttaa
     5761 tgctactatt tcattgctgc cataatactt aatatataac gttcttgtag ttaaatttat
     5821 tacggtctga tacatcgtat agtgattgtc agcatcatgc ggacgtacaa ttccaatcgg
     5881 tatatttacc gcatctaata aataaaatgc attcattaaa tccatttctt tatcattgtt
     5941 ttgagcaatg tttgctttca taaatgccat tctcacaaag cgctcagttg aagtaaatcc
     6001 acctggcaat ccaaatgtac ctgcttcatt gcctaaaggt tcaatcgtta caccttccaa
     6061 taactttgct gttgctggat aaggagaaat attgatatat tgtcttaaat tactataatg
     6121 ccaatctaag tctggatgat ttgttaagac accaataggg ttatctttta taaccacttc
     6181 cccctcttta aatgaaactt cgactgaatg tccagttgca tcggaaacat gataatgcaa
     6241 tggcggaact tcaccgatgt catttaaata taccgctaca acatgtattt gggatgcttg
     6301 ttgtttcata tcttcaatgc ttgttgtata tcccaaaatc catgtcacaa tttcattttg
     6361 cgtaatattc atcgcgtccg ctttgtgtgt tgagccatat gaactataac ctcggaaata
     6421 ttgtgtcgaa atagcaacgc catgttcatt aacaccatca ccataaataa aaccttccat
     6481 atctgttcct gtaccaataa agccatattg cgtttggcct gtcgtgccag tgcgagatgt
     6541 ccaacgataa tttctaggcg tcactgctgg cgaaccatct aatggataat cataatccat
     6601 cgtgcgtcca agaagtactt gattatttaa agtttgtatt gtgaatcctg tgcacattgt
     6661 tctcactcct ctgtaccttc atttacttta atcactttca aataaagctg tttcacttaa
     6721 acatactata aaaaatcaat catacaagca attaattgat attcattctc aataactgtg
     6781 gtatgatatg taaggaaatc atgacttatg tgtgagtgaa cgatcatcta tacatccgtt
     6841 cacttcatct catgactttc tatatttaat ttttacaagg agtgacatct gtgaataaca
     6901 cacaatcttc accacgcagt aatattatta ttgcgattat gttgtctgca ttaacatatt
     6961 ggttgtttgc acaatcattt attaatatag gacctctcgt tggtcaaacg tatcaaacct
     7021 ctcctgccgt gttaaattta tctattagtt taacttcctt cgccacaggt atcttcatgg
     7081 tggctgcagg tgatattgct gataaaatag gacaactgag aatgacatac atgggtctca
     7141 taatcagtat gtttgcatct cttctattaa ttatatcgga catcactgca ctgctcatca
     7201 tcggtagaat tttacaaggt ctatcagcag ctatcttgtt accttcaaca gttggcgttt
     7261 taaataatca atttaaagga gaacatttaa gacgagcgat tagttatcta atgattagta
     7321 ctgttggggg catcggccta gctggtgtta tcggcggttt aattgctaca aatttcggat
     7381 ggcaaatgaa tttcatcatt agtatagtca ttgctttcat tgccatatta cttctaaaag
     7441 gcacacctga aaaagtaagt caacatagcc accgtcatcc gttcgattac aaaggtatgt
     7501 cgattttcgc tgttatgatt ggtagcttta cattattgtt aacacaagga ttcgaacaag
     7561 gttggtttag tacattctca ttcatttgtc tgagcatttt tatcatcact acgctgatat
     7621 tcatcatcat cgaacgtcga catgaagtac cttttattga tttctcagta ttacgcaacc
     7681 gtccgttcat tggtgcattt ttaaataact ttgttttaaa tagcggtcta ggcgttacag
     7741 tggtcttttt catatatgct caaacacacc ttggtttatc agctgcgcaa tctggacttg
     7801 ttacattgcc atatgccatt gtggcagttg cgatgattcg tttaggtgaa aaagcaacat
     7861 tacgtttcgg tggcaaattg atgctcatca ttggtccgtt gttccctgtc atcggcatca
     7921 ctattattag catgactcaa ttatcggcat cacaatatgt cattgcagtt atcattggtt
     7981 tcgtcatttg cgcaattgga aatggtttag tcgcaacacc cggccttaca attgcaattt
     8041 tcagtatgcc aaatgaaaaa gttggtttag ctacaggatt atataaaatg agtggtacat
     8101 taggtggcgc ctttggtata gcactaagta ctacagtttt cagtatgtta caactaaact
     8161 atgcaccaag tgtagctgca accgtaacat ttatcgtcag cattgtattg atgatccttg
     8221 gctcattgtc tgcatacatg atcattccaa aaacagttaa atcttaaata taatagaaga
     8281 attatgtttc gaaatatctt tatcacttta aaatgatata caagaaattc aagaaaaata
     8341 agcgaagaac gtttttctct gttcttcgct tactctaatt atattcacta tcaattagac
     8401 aaaatggcca ttttcaaata tcacgcgttg tttctgacct tgaatatatt tattataatt
     8461 ctctttttga aaatcagtta acattaattt agatgtaccg tattttaaca ctttttgcat
     8521 tgtttctatt ctcatttttc taaataacca tccatctttt aacacaatac gattaacagc
     8581 atcatatgat aattctactg tttctttaat ttcaaatgtc ttgaatgaaa taatcgtgca
     8641 cattaaaaac gtatcaccaa agtaataaac atctaaatca tcacgtttat gttgtccaac
     8701 taacaaacga ccatattcga actctttttc tggatatttc aattctaaaa aactaataat
     8761 ctcttcttct tttaatttga attgcattta aaaacatcct ctcttaagtt ttaacaagcc
     8821 ttaattaaaa attttttcaa tcacatagtt caatatacat catttcgtta tgttttttaa
     8881 tactttgttc aaaaacaaat attttattct ttaaaataat gacttttgta tttttcatca
     8941 caataaacat tttaaaattc ttgttatcat aatcattaaa aggtattaac cttaataata
     9001 tattctctcg tctcaacctt aatcgtatac ttcagacgtc tgtttgtaga caataaaagt
     9061 cattcacgtc ttcatatgtc atcaaatgtt tatcatgata tgatgaatat aataatcggg
     9121 tatataactg tatgattaat tacacaataa agagaaggaa tataatatga aaaaagtgtc
     9181 aattaaagat gttgctagag aagctggtgt atcagttaca actgtgtcac atattttaaa
     9241 tcataatgat agtcgttttt ccgcaacaac gataaaaaac gtacatgctg tttcagaacg
     9301 tttaggctat gcccctaata aacatgcaaa acaattgcgc ggcagtaaaa ttcaaactat
     9361 tggcgtcatt ttacctagct taacaaatcc gtttttctca gcactgatgc aaagtattca
     9421 tgaccataaa ccatctgatg ttgatttatg ctttttaaca tctacagcaa ctgatttgta
     9481 tgacaatatt aaacatttaa ttgatcgagg tattgacgga ttaattatcg cacaatacat
     9541 atcatccccg gacgccctaa ataactatct aaagaaacat catgtacctt atgtcgtact
     9601 ggatcaaaat gaccatcaag gctatacaga ttttgttcgg acaaatgaat atcaaggtgg
     9661 acaacttgca gcacaacatt taatagaact cggtcacaac catatgataa ttgttgcacc
     9721 atatgacatg atggcgaata tgtcgactcg tgtcgctgga tttgtcgata ctttgcgcgc
     9781 gaatcaattg ccagaaccac aaatcgtcca tactgaatta tctaagcgcg gtgggctaac
     9841 cattgttgat gacatcatgg ttcaatctgc cactgcaatc ttcgctatta acgatgaact
     9901 cgctattggc attttacgag gactaattga acatggcatc agtatcccga aagatatctc
     9961 attaataggt tatgacgaca ttgattatgc agcgtacgtc tcgccacctt taactactgt
    10021 ggcacaacct ataactgata ttggcaaaac atctttaacc ttattacttc aacgattaca
    10081 gcacttagat aaatccattg atatgattga attaccaacg actttaaaaa ttcgtgcaac
    10141 aactggctat catctttcaa actaactacg tatcttccga aatatactca tcattgttag
    10201 gcccttagcg ttgctttaat gctgagggtt tttaatcata attatttcac taagaaatta
    10261 aaataataat gtatgaattt ttaaatatga tttaaacgtt ttcagttttt atgaaaacgc
    10321 atgcattttg caaataaaaa tggtacgatg gcattggtaa aacgttttac taaaaacaaa
    10381 tcgtgaggtg tataacatga gcattgttgc attacttatc gggttaggcc ccttaattgg
    10441 ctggggcttc ttcccaacag tcgcttcaaa gtttggtggt aaacctgtac atcaaattat
    10501 cggtgctact gtaggtacgt taatcttcgc tattatatta gccgtagtca catcaagtgg
    10561 cttccctact ggaaccaatt tgctattcgc cttattatca ggtgcaggat ggggattcgg
    10621 acaaatcatt acatttaaag cgttcgaatt aatcggctca tctcgtgcca tgccagtcac
    10681 aacagcattc caattattag gcgcatcttt atggggtgtc tttgcattag gaaattggcc
    10741 aggcattggt cataaaatca ttggatttac agctttagtc gttattctaa ttggtgcacg
    10801 tatgacagtt tggagtgaac gcaaagaagc aagtaacgcc aaaaatttac gtcgtgcagt
    10861 ggtacttctg ttaattggtg aatttggata ctggttatat tcagctgcac cgcaagcaac
    10921 ttctattgat ggcctaactg cctttttacc tcaagcaatg ggtatggtaa ttgttgcagt
    10981 catttatggc tttatgaata tgaaatcaga gaatccattc cgtaataaaa ttacgtggtt
    11041 acaaattatt tcaggtttct tctttgcatt tggtgcctta acatatctta tttcagcaca
    11101 acctaatatg aatggtttag caactggatt tattctttct caaacatccg ttgtgcttgc
    11161 tacattaact ggtatttatt tcttaaaaca acataaaact tcaaaagaaa tggttattac
    11221 aattatcggc ttagtactca ttttagtagc cgcttctgtt acagtattta taaaataagg
    11281 agtgtagatg tcatgaaaaa atcagctgtt ttaaatgaac atatttcaaa agcaatcgcg
    11341 acaattggtc attttgattt attaacgatt aatgacgctg gcatgccaat tccaaatgat
    11401 catcgccgta tcgacctagc tgtcacaaaa aacttaccac gctttattga tgtcttagct
    11461 acagtgttag aagaaatgga aatccaaaaa atatacttag cagaagaaat aaaagaacat
    11521 aaccctacac aattgcaaca aattaaacaa ttgatttcat cggaaatcga aatcattttc
    11581 attcctcacg aagaaatgaa aagtaactta gctcacccat taaataaagg taatattcgt
    11641 actggtgaaa caacgcccta ctctaatatt gcattagaat cgaatgttac tttttaaaaa
    11701 ttataacttg aaaggagcat acacatgacc aacaaagttg ttattttagg ttcaacgaat
    11761 gtcgatcaat ttttaacagt tgaaagatat gcacaaccag gcgaaacatt acatgttgaa
    11821 gaagcacaaa aagcattcgg cggaggtaaa ggtgccaacc aggctattgc cactgcacgc
    11881 atgcaagcag acacaacatt tattactaaa attggcactg atggcgttgc tgatttcatc
    11941 ttagaagatt ttaaagcggc tcatattgat acatcatata ttatcaaaac aactgaagca
    12001 aaaacgggcc aagcctttat cactgtgaat gcagaaggac aaaacaccat ctatgtttat
    12061 ggtggtgcga atatgacgat gacacctgaa gatgttatta acgcaaaaga cgctataatc
    12121 aatgcagact ttgtcgttgc acaattagaa gtacccatcc cggctattat atctgcattt
    12181 gaaattgcca aggcacatgg tgtgacgaca gtattaaatc ctgcaccagc gaaagcatta
    12241 cctaatgaat tattatcatt aatcgatatt attgtgccaa acgaaacaga agccgaattg
    12301 ttatctggga ttaaagtaac taatgaacaa tctatgaaag acaatgccaa ttacttttta
    12361 tctttaggca ttaagactgt tttgattacg ctaggtaagc aaggtacata ttttgctact
    12421 aaaaatcaaa gccaacacat cgaagcttat aaagtaaatg cgattgatac aactgctgca
    12481 ggcgacacat ttattggtgc atttgtcagt cgcttaaaca agtcgcaaga taacttagca
    12541 gatgctattg attttggtaa taaagcgagc tcactcactg tacaaaaaca cggcgcgcaa
    12601 gcatctattc ctctactaga agaagtaaat caagtttaaa tgaatcaaac acagctatga
    12661 tatgaaggtt tagcatataa catgcaacat tcgtatatca tggctgtgct tttttatctt
    12721 tataaaacat catctattag aaataatttt ccactaaacc tatgcttgtt gactcatgtt
    12781 tagttataaa tgaagtgaca attttttgta atctttttaa cttccaaatt attgcatata
    12841 aatatgctat attaatgata ataattatca attaaaagga ggttatgcta tgtctaaaga
    12901 agctggtcat acatttttag ctaaattagg aaaaactcgt ctacgccccg gtggtaaaga
    12961 agcaacagat tggttaatac aacaaggggc attttcacaa gataaacaag tgttagaagt
    13021 ggcatgtaat atgtgcacaa catctattta tctagctcat acatatggct gtcacattca
    13081 aggcgttgat ataaataaga aagcattaga aaaagcacag gaaaatattt cagcagcagg
    13141 tcttgaatca tatattcaag ttcaacaagc gaatgctgtt aaattgccct ttgatgacaa
    13201 tcaattcgat atcgttttaa atgaagcaat gttaacaatg ttacccatcg ccataaagga
    13261 aaaagcatta cgcgagtact accgagtctt aaagcctggg ggtatcttgt taacacatga
    13321 tattgtcatc gttaatgaat cacatgccac acatgttgtt aaatcattat ctgcagcaat
    13381 taatgtcaat gtctcaccgc agacgaaact tggctggtta gatttatatc atcaagctgg
    13441 ttttaatcat gtgcattatc atactggtcc aatgagttta atgacaccaa aaggtttaat
    13501 ttatgacgaa ggtattgttg gaactataaa gattatcaaa aatgctttaa aaaaagaaaa
    13561 tcgaccaatg ttttgtaaaa tgtttaaaac gatgactaaa ttgcgtaaag atatgaatta
    13621 tattactttt gtcgctaaaa aagaggacta aatataatgc cactaactgt actttgtatc
    13681 tatgtttgac tgctacttta atttctttgt gacactaatc atctacttaa caatatcgtt
    13741 atcgttgatt agtaagtcat caatttgggt taaagacttt cataaacact caaacattaa
    13801 cactatactt agttagtggc attatttttt taaaaatttt aacatcacgg gattggaaca
    13861 gaaatgatat tttcgcaaaa tttattcctg tccttcccca acttgcatta cctgtagaat
    13921 ttcttttcga aattctctgt gttggggccc cgccaacttg cattgcctgt agaatttctt
    13981 ttcgaatttc tctgtgttgg ggccccgcca acttgcatta cctgtagaat ttcttttcga
    14041 aattctttgt gttgggggcc cgccattata acactgtagg tcctactata ttaatttatg
    14101 tctcgggctc taaccctaaa atacttataa tataccttta taatttatct ccatttgatg
    14161 ctatcacttg ttgataccaa tagaacgaat cttttttcaa gcgttccatc gtgccatgac
    14221 catcattatc tcgatcaaca tatattaaac catagcgttt cttcatttca ccggttgtaa
    14281 atgaaacaat atcaatgatt ccccacggtg tataaccgat taaatctaca ccatcttgat
    14341 caactgcttc tatcgctgct gtaatatgtg cttttaaata ttcaatgcga taatcatcat
    14401 gaatatgtcc atctaccact tcatcaactg caccaaaacc attttccaca ataaaaagtg
    14461 gtaactgata acgatcatat aacacattca atgtatatct taagccatct ggatcaatcg
    14521 cccaacccca atcactcgtt gcgatatgcg gattctccac agaatgattc aaaccaccgt
    14581 tgacgatgtt gttttcaact gtagtatcaa catcatgttt tacagccgta gacatgtaat
    14641 aactaaagcc aatataatca accgtgccct gctgtaaaat actgtcgtcc ccttcttgcc
    14701 atccaacatc atatcctttt cgtgccaaca tttttttagc atagcttgga taataacctc
    14761 tcacttggac atccgggaaa aagaaacgta agcgattcgc aatttgtgct tccatcatat
    14821 ctttcggatg acacgaatat ggataaatgg gtacatgtga aatcattgtt ccaatcttaa
    14881 acttcggatt aatctcttta ccaagacgaa ctgctaaagc actggctaaa agttcatgat
    14941 gtgctacttg atacaagact tcttcaggat tatcattttc tgtcaatgct accccagaat
    15001 tcgtccataa aaagatagga tttgatgtgt ccatctgatt attaatttca ttaaacgtca
    15061 tccaatatgt aactttatct ttatatcttt caaatacaac acgcgcaaaa tgcacaaaat
    15121 aatcgacaac ttctctattt ctaaatccac cataatgttt cgctaaatga agtggcatct
    15181 caaaatgtga taacgtcaca acaggttcaa taccttgtgc aattaattca tcaaagatac
    15241 gatcataaaa ggcaagtcct tcttcatttg gcacatcttc atccccattc ggaaagatac
    15301 gtgtccacgc aatcgacgta cgtaaacatt tcaatcccat ttctttaaac aaggcaatat
    15361 cttccttata acgatgataa aaatcaatac cttcatgatt tggataatag tgattgaggt
    15421 ctatagattc tgtaatctga cgtgctttgc catgtgcacc actcgtcata acatcaatta
    15481 cacttaaccc tttaccacct ttttcatatc caccttcaaa ttgatttgcg gcaagagcgc
    15541 caccccacat aaaattttgc ggtaattttg tcattattac aacacactcc tagtttttaa
    15601 atattttaaa aataacatct tcagtcgtaa tgatttcatt cattgtcagt tctacttttc
    15661 ctaaatcggc agagttgcta ataacgacaa taacgtcagc attgtagcct tgttgttgta
    15721 tatattgctg gtcgaattgc aacaacgttt gccctgcttc aacatgatca ttttgcttta
    15781 caaagcactt gaacccttta ccttccaagt caactgtatt cacgccaata tgaatgacta
    15841 tgtccacacc gtcttctgat tgaataccaa ctgcatgctt agttggtaca atcatagata
    15901 ttaaaccatt gaacggtgcg attactttgg attcttcatg agctttgatt gctaagcctt
    15961 cacctaccat tttctctcta aaaattgaat ctcttacttc tttaagtaaa atacgacgac
    16021 cagcgattgg tgctttcact aaaattggtg ttgttacttt tacttgaggt gtataacttg
    16081 tatcttggct attaatattg tcagtattct cactctcttc gacattagca ttcttaatta
    16141 atcgctgtaa ttgctgatac acttcatcga catttccatt taattttatg actaactgcg
    16201 catcattttc tgctacagat gtcacatcaa ctatttgttt cacttcattt gctgtagaag
    16261 gaattgtatg ttgcatatga attgtaatgc acttcgtatc atacgttgca tcaacaatgt
    16321 tttcgacacc acctacagct gtaagaatgt cttgggcttg ttctttatat ttattgctca
    16381 taaatattac cccctcaact tgatagtaga atgaaaatgt atttacttac ctccaattat
    16441 aattgtaccg gttcaatttg taaacgccga tacaattata atattttgtg ctataataat
    16501 tacagacaaa gtgaaacgag gacagaatat tgttaaagta tgaacatatt gctaagcaac
    16561 ttaatgcgtt tatacatcaa tctaatttca aacccggtga taaattgcca aacgtgacgc
    16621 aattaaaaga acgttatcaa gtaagtaaga gtactatcat taaagcatta ggcttattgg
    16681 aacaagatgg tttgatctat caagcacaag gcagtggtat ttatgtgaga aatattgcag
    16741 atgccaatcg tatcaacgtc tttaagacta atggtttctc taaaagttta ggtgaacatc
    16801 gaatgacaag taaggtactt gtttttaagg agatggcaac gccacctaaa tctgtacaag
    16861 atgaactcca attaaatgca gatgataccg tctactattt agagcgatta agattcgtgg
    16921 acgatgatgt tttatgtatc gaatattctt attatcataa agaaatcgtg aaatatttaa
    16981 atgatgatat tgctaagggc tctatcttcg attatttaga atcaaacatg aaacttcgta
    17041 ttggtttttc agatattttc tttaatgtag ataaactcac ttcaagtgaa gcttcattac
    17101 tacaattgtc tacaggtgaa ccatgtttac gttaccacca gactttttat acaatgactg
    17161 gcaaaccctt tgattcgtct gacatcgtat ttcattatcg tcatgcacag ttttatattc
    17221 ctagtaaaaa gtaataaata cataaaaacg tctatatccc agttataaac tggagtatag
    17281 acgttttttt acgataataa caatggctca aattgctatt atcttgctta ggtttttcgt
    17341 tttagaagaa tattgctaca aagacaggca caactgctac aacaactaca ccaactaaca
    17401 ctaaagctat acttgccatt gattcttcta caggtcctaa ttctttggct ggtgctacac
    17461 ctaatgtgtg accacttgtt ccaagtgcta atcctcgggc aatagggtta gtaattcgga
    17521 aaagcttcaa gaatttatta cctagggcat aaataatgac accatttaaa ataactgcta
    17581 atgatgttaa ttcttttata ccaccgatac cagctgatac tggtaacgca atcgctgtag
    17641 ttgctgcttg aggtaacatt gataaaataa catcattggc aaattgtgct aacttcgcaa
    17701 aagttaaaat aattaataac gctacaactg taccgatacc aatacctcct atgatacgat
    17761 gccaatgttt aacaagcact tcacgctttt tatataacgg aatcgcaaaa cagattgttg
    17821 ccggttctaa gaagaagtaa ataatgtctc cacctatttt gtaagtctta tacggaatgc
    17881 ctgttaaata gaggaaggcc acaccaaata ccatactgac aaatagcggt gcgaataaga
    17941 agaaacgatt agttttttca aataatatgg tcgctaagaa aaatggtata acggataaca
    18001 gtattccgaa gtaaggtgtg tttagtgcta agtggttaat catgagcttg tgcctcctct
    18061 attttgatct tttttgtgac tttgtcacct ttagatctcg aagtaacttt cataataatt
    18121 tgtgtgacat agccagtaca aataagtaat agtattgttg agacgattat tagtccaatg
    18181 attaaaaatg gtgcttggct aatgacacct aaagagttaa caactgagat accggctggt
    18241 acgaagagta agccaatgtt atttgttagt gtcgttccta ctttttcgac ttcgcctaac
    18301 ttaacagcac cagtacataa taatacaaat aataatacta aaccgattac tgatgcaggc
    18361 ataggaattg gcataaatga ttcaattatt ttcgatacaa agagtactaa agcaattaca
    18421 atgacttggt gaaaaaagtg tgctggtttt gatgcgtctt tttgttgttt cacgaccatt
    18481 gcctcctacg tttgatttaa ctaaagtata gatggctcac ttcgatttgc gtgattttta
    18541 gtccgaaata caaaatatca taggtaaaat gcataaaaaa aaggattact gttaaagtaa
    18601 tcctatcgac gctttaaaat ctttcataaa tgaacgtcca acttgcatct tgacaccatt
    18661 tgtcaatatt accatataag tgtagttaaa ccattgttgc acttctttaa tgtgtttcgt
    18721 gttaataata tatgaacgat gaatacgtat aaaataagtg ggattcaatc gtttttcata
    18781 acgattcaat ggctctgttg tttcgtattt atgattcgtt gtatgtatgg ttgtaatacc
    18841 attatgtgtg ccaatcccaa taatattttg ttgctttaac atgtgaattt tatcgtcaat
    18901 ttcaacaggt aagctttgat caaaattcgc cgacatatca ttcgcaattg cacttgcgtt
    18961 attatcatct ttggctttag tcgcacgcac tttattgact gcttgttcaa tacgtttttg
    19021 accaaacggt ttcaaaatat agtctgtcgc atttaattca aatgcctgta ctgcgtattg
    19081 gtcatgtgca gttgcaaaaa taatcgcagg tggctctttc atcttttgaa tcttagctcc
    19141 taattcgatc ccattttcat ccattaaatt gacatctaaa aatataatgt catattgatt
    19201 gatcagtagt gcttccaatg tttcttttac attttctgcc tcattaattt cttcaaaacc
    19261 accaatttca tttaataaat atgttaattc attacgtgct aatggctcat catctatgat
    19321 taatgctttc atatttattc ctcctcttgt ctttcataag gaagtacaca ccaaaaagtg
    19381 gtaccgctcg atgtcgattc aaattgtaat gctgcggatt ttccaaataa tccttttagg
    19441 cgtaagttta aattttctaa agcactacca gttccagatt ctgattctac agatgtttct
    19501 cccaacaaat gcattttatc tttagaaata ccctgaccat tatcttgtac aataatacgt
    19561 acatgtgttg cagtttcttt aatcactgac acgtcaatat cgttaccttg ctttcgattc
    19621 gtaaacgcat gtttgatggc attttcaact aaaatttgaa ttaaaaatgg tggtacaagc
    19681 acatcgcgat atttgtcttc aacattaata ttgatattaa atcttcctgg ataacgtgct
    19741 tgttcgagtg ataagtatgc acgcacttga cttaactctt tatctaaagt aatcgtatgt
    19801 tgctttgagc cttgtaaatt cgctctgaaa aaataactca attctaatag taactctcgt
    19861 gccttttcgc tatttattct aactaaagct gaaatcgtgt taattgaatt gaagaaaaaa
    19921 tgtggactca cttgtgcctg taatgactta atctcagcat ctttcaataa cttactttgc
    19981 gtttcggctt caccaagttc aatttggcta ctaaaaatat ttgccaatcc ttctgcaagt
    20041 tgacgttcca caaaagttaa atcattaggg tttgtaaaat acatcttcaa tgtaccgacg
    20101 atagaaccat gcatctcaag tggtatcacg atagctgctc taagcgggca attcggatga
    20161 ctacaaccaa tctcttcttt agtatgcact tctttcaact ttcctgattt caatacatct
    20221 ttagacagac ttgttaatat ttcatttgtt ggtatgtgat gatcactacc tgcacctaca
    20281 tgcgataaga tttcattttt gcttgtaatt gctacggcag atactttcat taaattttta
    20341 ataatcatcg caatttgctg tgccgattct ctattcaatc cttctttaaa atacggcaat
    20401 gtctggttca tcaattgcag tacgtcatgt gtttgaacag ccttcatttg ctcttcttgc
    20461 tttaatgttg aaatgataat agacataaaa atcgccgtac caacgctatt aacaataatc
    20521 attggtagtg caattaatga tatgaggtca accgcatatg ctttgtcgtg ggaaaatgtt
    20581 aaaatgctca acatttgaat catttccata acaattccaa tcatggcact tttcgcaata
    20641 ctcgggtaac gcttgcgtct ttgagcttgt aagccaaaat aaccagcaat tataccgata
    20701 aatatagatg agataagata aacttgtgca tccgccccac ccatatacac tctgaaaata
    20761 cctgaaataa cgccaacaaa tagacctaca aaagggccac caactaatcc tgcgacacct
    20821 atcgttaata cacgtgtgtt agctaaagat acatcatcat ctaaacggaa gtacacactt
    20881 cctgacaaac tatgttgatg atcgatgacg ataccagtta aattagacat taaggcaaac
    20941 aaactgaaaa taatacataa ttgccaacgt gctttccatg tacgtcgacg attcattaag
    21001 tttttaaaat atggaatatt catcaacaca taggccaaaa taataattaa acctacacgc
    21061 tcaagtaata acattgttag cgatagcacg atagcacctc agtgaattat aattatgttt
    21121 attttatcat aatagtgcat gtaaaattca tgaataaatt attaaaaaaa cacgctacta
    21181 tttaattatc tataccatta aattttcata tcaattttta tgctaagttg accatttttc
    21241 ttttcaaata atgctgttca tatcactaaa tttctgttaa gtatagcgaa aaactgctga
    21301 cattactttg ccaacagttt ctaatcgcgt tatttatgat acttttttaa ataaaacatg
    21361 attctctaaa tgaacatgct catgtgttaa cacttcaagt gcttttaatc tctgataaac
    21421 gagtcgccac gtaccacatg cctctatcgg tggttcatag ctagatgtta aatcgctcat
    21481 tttcactaac aattgtcccg ttgcaatgtg atcagatact aaatcatcaa tcactgtatt
    21541 aatatcgttt actacttcac cttgttcata tttaattagt tttggaaaat caacatcatc
    21601 ttctttttgc atatgctcta acatgccact tttaaatgta tcatatgttt cttttaactc
    21661 gactaaatat ggatggttag gtccatgtac tttcgataat ttcgtcacat aaggtgttaa
    21721 atttttaaat tcttctctaa gaggttcatg atatgctgct tgaatatatt gaataagtga
    21781 cgaaacattt aaaaatttag ggttaagcga acctggtgta ttcgtttgtt caacgtcatt
    21841 gagacgctgt aataattcgt tcaaatctac atttttcttt tctaaggatg ctgcttctat
    21901 acttacttgt ccaccgcaac aaaagtctat acctacgctt ctaaaaatat ctgctgcttt
    21961 cggataatct attactatat ctgctactat gtcattttta tttatcatta tagacgacct
    22021 cctcattttc agtgtataca atttaatgta tatgaaaatg aaaatcaggg aaatgtcata
    22081 atcatttcat aaatagcaaa ataaaaccct caaattatgt tagttcacat agatttgagg
    22141 gcatttatta tttattttag cgagtaagtc atatgtgcag tgtttaactt aacaatattt
    22201 ttgaagtctc gcttcttaat atctttacta agtaagattt ccacttcgtt ttctgacgtt
    22261 tggcttaaca ttcccataat catgtcgtct tttagcacga cactagccat accttcttca
    22321 aatacataac cacgatccag accagttaaa atgattttgt ttttaccttt taacttcatt
    22381 gagattgaat ccgttaatgt aatagctgta aagtataacg aaatgttatt aaagtctttt
    22441 gtgaaatacg gtgttaatct ataaaaatca ttatttaaat gaattaatgc acttgtttct
    22501 gcagtatatg catatttatt acgttgatta ccaacacgca cttcaaatac ttccgtacct
    22561 atttggactt ctaagaaaac atcccaaatt ttttctttag gtaaatacgc tttaatatct
    22621 tctaacataa tgttaaaacg atattgttta ttcgcattga cacttaaagg tacatacatt
    22681 gtgtgcttct tgtttcgtga atgtaattta agtctgacat cataatgtcc ttcgccaatc
    22741 ataaattcat ttaacgttag ctctgtaaca attttatgat tatcaaattt aaatgctgtc
    22801 attttcactt ttggcttagc gatatcaatg ccttcaaagt atggttcgcc ttcattgaat
    22861 tgaatgttta atttaccatc tacaatatca aaacggtagt attgaccttg acggtaactt
    22921 tcttctacag ttctatagtt gtctatatct ttcgctctcg cgtagtgcaa caatggtcgt
    22981 aatttactca tcaccaatgc atctacacgt tctggaactg cttgtataaa gtctcctaga
    23041 gcattaatcc attgtggttg ctcttcaagt ttaactttaa gtgagaagcc attcgtacga
    23101 gaaaaactaa aatgacgatt taaaaattct gctaagattt gatctttatg agcttcttct
    23161 aaatctgcat ttaatatttc tacagcaatc aatctcatga cttcgtaaaa gtcttcaggt
    23221 gaaacatacg cactactcat atgttcacct tcacgctttg tagcataata atacgcttta
    23281 tcacttaaca cactgattcg atttgcattc aaatatgctt tcattgtaaa taattgatct
    23341 tctgcactct ttaattcttc tggaaaataa atgtcattat cttttagtaa tgctgttcta
    23401 tagattttag tcgggcttaa agtatagata attcttgaat ttaacaaagt aacttcaggt
    23461 gctgtttctt taaacataga ttgtggtaca ccacgaccat taacaccttt cattttaatc
    23521 aataagacat ctgagtgatg ttcatcaata aatgctgctg catcctttaa agtctctttg
    23581 tttatatagt catcggagtc cacaaataat acaaattcac cttctgcttg ttttaacgcc
    23641 acatttctag gtttacctgg accaccagaa tttgtttcga gttgacttac tttcaagttc
    23701 aatttattgc gatacttttc aactatttgt aacgtttgat ctgttgaaca gtcatcaact
    23761 acaaccactt caaattcagt tttcggaaaa tcttgtttcg caaggctatt aagtaattct
    23821 gttatatact tttctgaatt gtatgttgga actattactg aaaatttcat cattatacct
    23881 ctcccacttt gactactata taaacttagc taccaaataa atttctgact aaacgctcac
    23941 ttgatcggcc atcttgatat ttaaaatgtt tatctaagaa tggaatgact ttttctcctt
    24001 cataatcttc attgtccaag gcgtccatta atgcgtcaaa tgattgcaca attttacctg
    24061 gaacaaatga ttcatatggt tcataaaaat cacgcgtcgt aatataatct tctaaatcaa
    24121 atgcatagaa aatcattggc tttttaaata ctgcatattc atatattaaa gatgaatagt
    24181 cactaattaa taaatctgtt atgaacagta tatcattaac ttctctaaag tcagaaacgt
    24241 caacaaaata ttgtttatgt ttgtctgcaa tattaagtct atttttcaca aatggatgca
    24301 ttttaaataa tacaaccgcg ttattttttt cgcaatatct tgctaaacgt tcgaaatcaa
    24361 ttttgaaaaa tgggtaatgt gctgtaccat gaccactacc tctaaatgtt ggtgcgaaaa
    24421 gaatgacttt cttaccttta ataattggta attcatcttc catctcttgt ttgatctgtg
    24481 tcgcataagc ttcatcaaat agtacatcag tacgtggaac acctgtaggc actacatttt
    24541 tctctttaat accaaatgct tcagcgtaga atggaatatc ggtttcagat gatacataag
    24601 cttttgtata gctacgatga tttaatgaat caataaatgg tccacccttt ttaccagtac
    24661 gactaaagcc aactgtttta aaggcaccaa cggcatgcca tacttgaata acttcttgag
    24721 aacgtctaaa acgcactgta taaatcaatg ggtgaaagtc atcaacaaag atgtagtctg
    24781 ccttcccaag taaatatggc aatctaaact tgtcgatgat gccacgtcta tctgtaatat
    24841 tcgctttaaa aacagtgtga atatcatact ttttatctaa attttgacgt aacatttcgt
    24901 tatagatgta ttcaaagttt ccagacatcg ttggtctaga gtctgatgtg aacaacaccg
    24961 tattcccttt tttcaagtgg aaaaatttcg tcgtattaaa tatcgcttta aaaataaatt
    25021 gtcttgtatt aaatgattgt ttgcggaaat acttacgtaa ttctttatat ttacgaacga
    25081 tataaatact tttaacttcc ggagtcgtta caacaacatc aaggacaaat tcattaacat
    25141 cgctagaaat ttcaggtgta acagtataaa ccgttttctt cgaaatgccg ccttttctaa
    25201 attcttttag gtaagtctgc aataagaaat tgattttacc attttgtgtt tctaattcgt
    25261 tgtattcttc ttcttgttct ggctttagat tttgatatgc atcattaatc acatctgggt
    25321 ttaactgtgc aatataatca agttcttgct cattcactaa taagtactta tcttcaggta
    25381 agtaataacc attatctaag atagctacat tgaaacgaca aacgaattga ttcccatcta
    25441 ttttgacatc attcgccttc attgtacgtg tctcagttaa atttcttaat acaaaattac
    25501 tatcttctaa atctaggttt tcactatgtc cttcaacgaa taactgaaca cgttcccaat
    25561 agattttatc tatatatatc ttacttttaa ccaacgttaa ttcatccttt tctatttaca
    25621 taatccattt taatactgtt ttaccccaag atgtagacag gtctgcttca aaagcttctg
    25681 taagatcatt aattgttgca atttcatatt cttgaccttt taacaacgct aatttatcta
    25741 caatatctgg gtattgaatg tataagtcta caacatcttg gaaatctttt gaaccacttc
    25801 gactactacc aatcaacgtt agtccttttt ccaatactag acgtgtatta acttctactg
    25861 ggaactcact tacacctaac agtgcaatgc ttccttctgg tgaaatataa tcaatcattt
    25921 gatttattgc tgattggcta ccacgcccac caacgcactc aaatgcatga tcaaatgtta
    25981 agccttcagg tattttatta ataaagaaga catcatcaac aaatgagaag tgactcaatt
    26041 tataatctgt tttaccaaag acatatattt tagactctgg atataattta cgtaataaaa
    26101 tggctgtaat gtaacctaag ttaccatcac cccaaatacc aaatgtattt ttatttgaaa
    26161 tagatttctt ttcaaaacga cgaatagcat gcaaacttac tgttacaagc tctgtatatg
    26221 aaataatact taaatcaata tcatcaggta gtggtacagc tctatcatga tttagcaaca
    26281 caaaatcttg cataaaccca tcatgtccac ttgatctgaa gtagctcgat tttaaatagt
    26341 tttcagcaat gacatcgtct ttttctgtcg gcgtattcgg taccataact acttttgtac
    26401 ctttattaaa cacaccttta ctgtcaaata cgacctcacc aacaccttca tgaattaaag
    26461 acattggcaa tttctgagat aagacattct catcacggct accagtataa tatctttgat
    26521 cggcagcaca aattgacata tataaaggac gtacaatgac atagtcactg taaatatcta
    26581 cgttgttata cgtaacttca aattgtctag gtgcaactaa ttgatatact tgattaatca
    26641 tcggcaatac cacctcgaat aatagcattc gctactttta aatcgtaagg tgttgttact
    26701 ttaatgttat ataactcacc ttttacaagt cgaaccggtt tgtttgtttc tacaataatc
    26761 ttacaagcat cagataaaat actcttttgc tcatcactca actgtgcata gctttctttt
    26821 aataaattaa tattaaacga ttgaggtgtt tgaccttggt acatttcatt acgcactgga
    26881 attgcatcaa tcgtttgatt atctttagat gtaacaatcg tatctatagc atcaatcact
    26941 gtatctactg caccgtactc taaagcagct tgaatatttt ctttaataat acgatgcgtt
    27001 aaaaatggtc taactgcatc atgtgtcaca atgacatcgt catcgttaat accatttgtt
    27061 gattcaatat gtttaacgat attcataatt gtatcgttac gatcgctacc accttgaatg
    27121 acttcaattc tttcatcaga aattttgaat tttctaagtg tatctttcgt atgcgtcatc
    27181 cattgttgtg gcgtcgcgat aataattttt tcaaaatcat taattaaaat aaatttttct
    27241 aatgtatgga ttaaaatcgg tttgttgtct aaatctaaaa attgtttagg taaaggtacg
    27301 ttacccattc ttgagcctat acctccagct agaataccag cgtatttcat aaaatacttc
    27361 ctccattcaa ctatatctat atttaattat ttaaatttcg ttgcattttc caattgaaaa
    27421 ctcattttaa aatcaaaact ctaaatgtct gtgtattact taaaattata catattttgc
    27481 ttatatttta gcatattttg tttaaaccta tattacatta tatcagacgt tttcatacac
    27541 aaataataac atacaagcaa acatttcgtt tattatttat atcacttaac taattaattt
    27601 ataatttttt attgttttta agttatcact taaaaatcgt ttggcaaatt cgttgtgacg
    27661 cttgtccatc ttctaatgaa cagaattttt gataaaatac cgttcgtgct tcaatatact
    27721 catttgcagt ctcatcgatt tgttttaatg catcaatgag tgctgtttga ttttcaacaa
    27781 ttggacctgg caactctttt ttataatcca tgtaaaaacc tctaagctca tcgccatatt
    27841 tatctaagtc atatgcatag aaaatttgcg gacgctttaa tacaccgaag tcgaacatga
    27901 cagatgagta gtcggtaact aacgcatcgc tgattaagta taaatccgaa atgtcttcat
    27961 aatctgaaac gtctttcaca aaatcatcat gttcatcaat acgtgtcaca actaaataat
    28021 gcatgcgtaa taaaataaca taatcatcat ccagcgcttg acgcaaagct tctatatcaa
    28081 agttaacatt aaattgatat gaaccttctc gaatcgcttc atcgtcacgc caagttggcg
    28141 cgtacataat cactttttta tctaatggaa tatttaatct tgtcttaata ccattaatat
    28201 attcagtatc attgcgttta tgtgataatt tatcatttct tggataacct gtttccaaaa
    28261 tcttatctcg actaacatga aatgcatgtt gaaatatcga tgtcgaatat ggattaggtg
    28321 acactagata atcccaccgt tggctttctt ttttaaagcc atcttggtaa ttttgagtat
    28381 ttgttcctag cattttaacg ttactaatat ccaaaccaat cttttttaat ggcgtgccat
    28441 gccatgtttg taagtacgtc gttcgcggtg atttatataa ccaatctggt gtacgtgtgt
    28501 taatcatcca cgctttcgct cttggcatcg ctaaaaacca tttcattgaa aactttgtaa
    28561 catatggtac attgtgctgt tggaatatgt gttcatatcc ttttttcaca ccccatatta
    28621 attgggcatc gctatgttca gttaagtatt catataatgc tttggggttg tcgctgtatt
    28681 gtttaccatg aaagctttca aaataaatta gattcttgtt tggcaatttt gatagtaatt
    28741 taaaagtcgt atatatacta tgttctatca attttttaat tgtattttta atcatgtcgt
    28801 acctccgacg tgtttttgta attatattaa tatgtatgag caagctcatt gtaaccatgc
    28861 ctattatagc atttcatcat aaaatacatt taaccattac acttgtcgtt aattatcata
    28921 cgaaatacat gattaatgta ccactttaac ataacaaaaa atcgttatcc attcataacg
    28981 tatgtgttta cacatttatg aattagataa cgattggatc gattatttta ttttacaaaa
    29041 tgacaattca gttggaaggt gattgctttt gattgaatcg ccttatgcat gaaaaatcaa
    29101 aaggttattc tcattgtata gtcctgcttc tcatcatgac atgttgctca cttcattgtc
    29161 agaacccttc ttgaaaacta tgccttatga ctcatttgca tggcaagtaa tatatgccaa
    29221 cattagcgtc taaacaaatc tttgactaaa cgttcacttg agcgaccatc ttgatattta
    29281 aaatgtttat ctaagaatgg tacaactttt tcaacctcat aatcttcagt gtccaaagca
    29341 tccattaatg catcaaagga ttgtacaatt ttacctggaa caaatgattc atatggttca
    29401 tagaaatcac gcgtcgtaat gtaatcttct aagtcaaatg catagaaaat catcggcttt
    29461 ttaaatactg catattcata tattaaagat gaataatcac taatcaacaa gtctgtaaca
    29521 aagagaatat cgttaacttc acgatgatct gacacatcga taaagtattg tctatgttca
    29581 cgtgaaatat taagtctatt ttttacgaac ggatgcattt tgaataacac aactgcatta
    29641 tgcttctcgc agtatcttgc taaacgttca aaatcaattt taaaaaatgg ataatgtgcc
    29701 gtaccgtgac cattacctct aaatgtcggt gcgaatagaa taactttctt accttttata
    29761 attggcaatt catcttccat ctcttgttta atttgtgttg cataagcttc atcaaatagt
    29821 acatcagtac gtggtacacc tgttggtaca acattttctt ctctaattcc aaatgcttca
    29881 gcataaaatg gaatatcggt ttctgatgaa acatatgctt tcgtgtaact acgatggttt
    29941 aatgaatcga taaacggacc acctttttta cctgtacgac taaagccaac tgttttaaag
    30001 gcaccaacag catgccacac ttgaataatt tcttgtgatg gtctaaagcg aaccgtataa
    30061 attaatggat ggaaatcatc aacaaaaata taatcggcct taccaagtaa atatggcaat
    30121 ctaaacttgt ctctccattt gcgtctatcc gtaatattcg ccttaaatac cgttttaata
    30181 tcataatcaa aatctacttt ttggcgtagt aactcatcat atacatactt gaaattccct
    30241 gataaattcg gacgcgaatc tgatgtgaat aatattgttt tgcttctttt aatatgtagt
    30301 aactttgtaa tattaaaaat agctttaaat aagaaacttc tactttcaaa tgaagcttta
    30361 tgaccttgtt tatgaagcca gtgtgcactt gtcgcaatga cccctgattt ctcttgaggt
    30421 aaggtgattt caatatcaaa tacaaattcg ttaacgtcac ttgctatttc cggagtaatg
    30481 gtatagaccg tatgttgtga tacgccacct ttacggaaaa ctttagcatc atacgctaat
    30541 aaatagttca cttttccatt ttgtgtagtc agctcattgt attcttcaat ttgctcttca
    30601 tttaatgccg cataagcttg atcaataata tttggattaa gttgtccaat atactctaac
    30661 tggtcatgat aaacaaataa atatttatcc attggtaaat aatacccatc gtctaatatt
    30721 gcgacgttga atctacatat aaatgtatta ccgtctattt tcatttcatt tgcttctaat
    30781 gtttttgttt cggttaaatt cctcagaaca aattgtcctg atgttaaatc gacaccttca
    30841 ctatgtcctt caatatataa ctgaacacgt tcccagtata tgttatcaat atgtattgct
    30901 tgtttcgttt ttgtcattgt tattccccca tcggtttatt aaatcgtcca tttcaatact
    30961 gtttttcccc aagatgtcga taaatccatt tcaaacgctt ggacgatatc ttgcatcgta
    31021 catacattaa tttcatgtcc ttttaataat gctaactttt caactatgtc tgggtactta
    31081 cgatataaat caacaacttg ctcaaaatct ttagagccgc ttcgactact accaatcaac
    31141 gttaatcctt tttcaagtac taatcgtgta ttcacttcca cgggtaattc acttacgcct
    31201 aacaaagcaa tactgccttc tggtgaaata tgttcaacta tttgttgaag tgcaacttga
    31261 cttcctttac ctccaacaca ttcaaatgca tgatcaattt taagatcatc tggtatttga
    31321 tttactgtaa agatgtcatc tacaaatgaa aaatgactta atttatagtc tgtcttacca
    31381 aatacataaa ttttagcttc tgggtacaac ttacgtagca aaatagcagt aatataacct
    31441 aagttaccat caccccaaat accaaagctg tttttcaaag gtatagattt acgttcaaat
    31501 cgttgtatag catgataact tactgacact aactctgtgt atgaaatcgt actcaaatca
    31561 atgtcattag gcagcggaac gatacgatca tgtgccatca caacgtagtc ttgcataaaa
    31621 ccatcataac cactagatct aaaataacta gaggctaagt aattctccgc gataatatga
    31681 tgttgctctg taggtgtatt cggcaccatt actactttcg tacctttttc aaatactcct
    31741 ttactatcga atacaacttc accaacagct tcatgaacta atgacattgg caattttttg
    31801 cgcagtacat tttcatctct tcgacctgtg taataccttt gatcagctgc acaaatagac
    31861 aagtataaag gtcttacgat gacatgatta ccataaatat caacattatt atatgtgacg
    31921 tcgaactgtc tcggtgcaac gagttgatat acttgattaa tcatcggcaa tatcaccttg
    31981 aataatggca tttgctactt ttaaatcata cggtgttgtc actttaatgt tgtatagttc
    32041 tccacgtacc aatttaactg catgtccaga ttcgacaatt attttacatg catctgataa
    32101 gatttctttt tgttcactac ttaaggcgcg ataactatct tgtaataatt taatattaaa
    32161 tgattgtggt gtttggcctt gatacatttc attccttaca gggatactgt gtatgttctg
    32221 tttatcttta gacattacaa tcgtatcaat tgcttcaatg actgtatcta ctgcaccata
    32281 ttttgctgct acttcaatgt tctctttaat aatacgttga gttaaaaatg gtcttacggc
    32341 atcatgagtt acaatcacat catcattatt aattccattt acattgcgaa tatggtcgat
    32401 aatgttcata attgtttcgt ttcgatccgt accacctgca actactttga cacgttgatc
    32461 tgtaatgtta tattttttta aaatatcctg tgtatgggaa atccactgtg ctggcgttgc
    32521 gataataatc tcattaaatt cactcactaa aatgaacttc tcaattgtat ggattaaaat
    32581 cggtttatta tcaatatcta aaaattgttt tggtaatggc acgttcccca ttctcgaacc
    32641 aatacctcct gctaaaatac ctgcataaat catgttgtcc tccattctgt cattacatca
    32701 tttccattta tacattactg acctatgccc gcacataagc ctaacctatt gctcacttgc
    32761 ctcttttatt aatccaaaga tagttgtcac aatagtgtga taatttttta taaaaatgta
    32821 tttttgtaac tgaccattct aagttgtttt gccatgcagt taatcattaa ctctgacgat
    32881 attaaattgt taaaggtatt aatgtttact ctttttcaaa ttcattatta ctgccatcat
    32941 tttaccatat attataataa atttatctta ttaagtggct gtacttgatt ttcactttaa
    33001 aaattatcaa atattgccat ctcattttaa gtatacaaaa tgcaaaacaa ccgattcaca
    33061 agcatatttc acacaagtaa accggctatt tatcaacgta tattcgaaga tgaattattt
    33121 cgatagtatc tgtagaccag acggcattcg cactttcata gctataacta taccagcgtt
    33181 ttcgtcctca aaggtgcata ctaataaatc gtaaacatga ctttatcaaa tcgttctttc
    33241 ttgttaacta atttatcaaa tgtctccggg cctttttcta acggtaaaaa atgagaaata
    33301 ataggcttta cattaatatc tttcgtcttc atataatgta aggttgccgt ccactctttg
    33361 cccggaaaat tactggacaa acagttccaa gagccacata ctgtcaactc gttacgcaga
    33421 attttttcaa aatgaacgcg atcaatctca atatcatcat atggtattcc gagtaatacc
    33481 acctcgccac cttttttagg tagcgtcaat atttgaccaa tcgtaacttt agcacctgat
    33541 gattctatag ctaaatcgat ttgattggcg taatgatttt cgatgaattt ctcaagattt
    33601 tcttcttttg aattgattgt ttgatgtgcg cccaatgatg ttgcaatatc tagtttatgc
    33661 gcatctatat ctatagcgat gatatgtgca gcaccaaata ttcgtgccca ttgaatagct
    33721 aacaaaccta tactgccaca ccccattact gcaacagtca taccaggttg tatattcgat
    33781 ttataaaacc catgcgcaac aacggctgat ggctcaacca ttgctgcttc aatgtaatca
    33841 acattgtctg gaacctttaa aacattttgc gctggcaatt tgacatattc cgcgaacgat
    33901 ccaggttcat atgagccaat gacgaataac ttttcacatc gtgcatattc acctttcaaa
    33961 caatactcgc attgataaca aggtattgct gggcaacctg tcactttgtc gcccacatta
    34021 acatgcgtaa catcacttcc aatggcatct actacacctg aaaattcatg accaaatggc
    34081 atacctttaa tgtatggccc cattttttgt atcgtgacgt gtctgaacca catatgccag
    34141 tcgctcgtac tttaacaata acgtcattcg cactttcaat gactggcttt tcattatcct
    34201 cataccgtaa atcttctacg ccatataatt tcaatgcttt cacttgtaaa tcacctcaaa
    34261 tttgatttaa ttcacaactt ttttcttttt aaaaatacct gtcgcaaaat aacctgcaat
    34321 gacgatggaa ttacttacga gtaaatgttc catataaaaa tcagtgattt gtcttaatgg
    34381 cccaagcata aaagttagca ttgattctac catattaatt cactccttca ttttcatcta
    34441 aatcgagcgg cttaagtaat gctttaatac cttcgccaga ttttaaatgt tgatacgcct
    34501 cgtcccattt cgaaatatca tatatttttg tcaccaaagc ttcagcattt actaaaccat
    34561 ccgccataag ttgcaatgaa ggttcccaat ctgctggctt ttgacttcta ctaccaacaa
    34621 ctgttatttc tttttgaatc actttttcca tatcaaatgg aatttcagca tccttaaaaa
    34681 tacctatttg actgtagaaa ccttttttgc gtataatatc taaaccttgt cgtgctgctg
    34741 gaactgcacc tgaacattca acaacaacat ctgcaccgta accgtctgta attccattga
    34801 tatacgtttt taagtctgtt tgttgtaaat tgactacata atccatgtgc aatgcttctg
    34861 ctttatctaa tctgactttg tcattgtcca atccagttac cacaacagtt gcgcctttac
    34921 ttttcaacac ttgtgctaca agtaatccga ttggcccagg tcccattaca actgctacat
    34981 cgccagagtt cacttgaatc ttagaaacgc catgatgtgc acatgctaat ggttctgtca
    35041 tagctgcaga ctgatacgat acttcgtctg gaatatgatg caaactttct tcacgtgcaa
    35101 tgacataatt agtaaatgcg ccatcaactt gtgttccaat accttttcga tggttgcata
    35161 aattatagtc ttttgattta cagtattcac actcattaca aacatagaat gtagtttcag
    35221 aagtgacacg gtcaccaact ttaaaatctt taacgtctgc tccaacttca acgatttcac
    35281 cagaaaattc atgacctaat gtcactggaa aattaacttt ataatgccct tcataagtat
    35341 gaatgtctgt gccacaaatt cctgcataat gtactttaat ctttacttta tcatctagcg
    35401 gtgttgcaac ttctttatca agaagttcta agttgccatg tccttctctt gtttttacta
    35461 aagctttcac cacaaacacc tcgattttta attgaataga ctaaatagtt taaagataag
    35521 atagttaacg atattaccac cttgatcaat acttgaaatt tcagatgaac cttttggcat
    35581 ttgtacattc gtacctttcg ccatatctgt gaaaatgggt gctacgtctg ttgcaatata
    35641 taatgaaatt gcaatcataa tcgtacccac aatgacagaa tgaataatgt ttcctcttgc
    35701 tgcaccaaca ataaacgcga caacaaatgg tatagttgct aagtcaccaa aaggtagtac
    35761 ttggtttcct ggtaaaataa cggctaataa aacagtgata ggtactaaaa ttaatgctgt
    35821 cgaaataacc gctggatgac ctaatgctac agccgcatcc aatccaatat aaatttcacg
    35881 ttcgccaaaa cgtttattta gccatgttct tgcagactct gaaactggca ttaaaccttc
    35941 cattaagatt tttaccattc taggcattaa taccattact gcagccattg acattcctaa
    36001 attaatgatg tctccaggtt tgtaacctgc taacacacca atacctaaac ctaaaattaa
    36061 gccgacaaat atagactctc caaatgcgcc aaaacgtttt tgaattgttt caggatcagc
    36121 atctaactta ttcagaccgg gtactttttg taacaattta actaagtaaa tacctggtgc
    36181 ataagaaatc gtgcttcctg tcgcaatcga tacacctggt aaatcaaaga actcactcat
    36241 catcggtgct gtccaatctg ctactttcaa acagataact tggaaaataa ctgctgctaa
    36301 taacgcttgc caaatactgc ctgatacggc ataaaccatt gctgctgtaa acgtataatg
    36361 ccaaaaattc caaatatcta cattcatcgt ctttgtcact ttagttacta gcaatacaac
    36421 gttaactatg attccgagtg gaataataaa tgctgcgaca gatgatgccc aagcgataga
    36481 tgatgttgct ggccaaccta catcaatcac attcagactg acgcctaaat ttttaaccat
    36541 cgcttgtgct gctggcccta aatttttaac taataaatcg atgactaaga aaatccctac
    36601 aaaagccaca cctattgtta aaccagacct aaatgccgct ccaattttct gcctaaagaa
    36661 taggccaagc aagaatatga caaccggtaa aataacagtt gcacctaaat ctaaaaatcc
    36721 ccttacaaaa tcagtgaagt aactcatatt taaaccctcc ctgttatata tgcattgtca
    36781 cgatactttc cgattgtgat tacatttgac gttacggtca tttcaacgac aacccttgct
    36841 acattcgact gcagtccctt tgaattacag tcactgcgtt tctatgtcat caacaatcat
    36901 ttgtcgtgat agtcatttat atgcaatttg catatattta tatgttattg acccacgtta
    36961 catatcaatt ccgttatttt tgtaactctg ttaagatttg ttgttttgtt tcttcaatac
    37021 caataccagt taagaaatta cgtgcgttga taactgggaa tttatattct ttctttgtca
    37081 ttgcagttgt aactaataaa tctgcagtgt cttcataagg tccaacttct gtaattttga
    37141 tttgtttaat atctacttta atattgtgtt cctttgccat ttcctcaatt gcattattta
    37201 ctactgttga cgttgcaata cctgcaccac acgctactaa tacttgtttc attttcaatt
    37261 cctccaatta atttttagtt atattccaaa taatcattga ttagtgttgc taaaatcgtt
    37321 tcatctttcg ttcgcagaat ctgctccaat ttttcttcac tttgaaaaat ttgcatcaac
    37381 tgttgtaaca acttaagttg atcatctact ttatccatcg ctaacataaa aacgattttc
    37441 acttctgtct gttgatcaag tgttcccatt tcaataaacg gcacctcttt ttctagaaca
    37501 gccacaccta tcgttctatg gttaatatgt tcgacatctg tatgcggtat agcgaccgaa
    37561 catagatgtg ttggtaaacc agtagcaaat tctttttctc tgtcgatgac tgcatcttta
    37621 aacgttgact tcacgaaccc attttgaaat aacacatctg acatttgtga caatacggat
    37681 tctttatcag ttgccgacaa attgagcatt atattttctt tatgcactaa ttgctgtccc
    37741 atccattttc cctcgcttct ttatttgaat aattttttaa aatctcattt acatcagaat
    37801 ttttgcgact ttgtatgatg cgcttaattg cgtcattgtc ttgcgccaca tctctcaatt
    37861 gtagtaacgc tcttaagtgt gtcactttat caacagcagc aataggtaca ataatatgga
    37921 ttgctgtgcc atctgacatg tatattggtt cttgtaatat caacatactc atcgctgttt
    37981 tatgtacatg cttttcagag tatgcatgtg gaatagcaat attttgcatg ataaccatat
    38041 acgaatcatc aaatacctgt tgcatctcat gaatatattt aatatcaata aaatgattag
    38101 caactaacac atcacttgct ttagcaatag cttcatcaat attttcaaca tgatgcattc
    38161 ttttcacgtg ccttgccggt atcaagtcag ctaaatctaa tgtcttattt tgtgtgacaa
    38221 tcgatccatt aatggttgaa attgaattat aattggcaat aaaatcttct aaaccatcac
    38281 gtagttctgt aatgtcatta actgtcgttg tgcgttcaat taatgccatt aacttgttta
    38341 tttccttatc aatgtcagcc gattccttat taatgtactt catcacttct ttacgtaact
    38401 ttcgttgctc attttcagat aaagctactt ttgtgataaa taatttttta tgtgttagga
    38461 caaacattgg tgacaagacg atgtcataat ctaatgtgta attttcaaat gttctaagtg
    38521 aaatcgcatc taagaaaata atttctggaa ataagtttcg caactcgtat aacatcattt
    38581 gtgatactga cgtgccttgt gtacacacga taatagcttt tatcttgcca tcgaagtttt
    38641 catcttgacg tctcaaacta cctccgaaca acatggttaa atatgctatt tcattatcag
    38701 gcaacgattt tccgaaatat tcagttaacg attgacatga ttgtttcacc atatgaaata
    38761 aggattgata atttccttgt aaaggattta ttaattcatc acgatccgtt aagttatatt
    38821 taatcctata aaaagcaggc gttaaatgta acaagagttg ctgtgataat ttctccttat
    38881 cttcaatgtt aataaaagtg atttgttcaa aatggtgaat catttgagcg atggccatcg
    38941 ttaaattcga tatgttatct gattcctgca aatcagtcca ttgcacactt gttgaaagta
    39001 agtgtaatgt caaatataac ttttccgctt ctggcaaatc aggctcatgt tgcgtcataa
    39061 tctccgttgc ttgatattct ttcgtatccc tcaaatactg ataattaata tttaatggat
    39121 tcatcacatg accactttga attcgtctac gaatcacaca aaggacataa ggcaatgaac
    39181 taagtgattt gtctataaag cgactcttca aaaattgttc tacctgtttg atcttgtctt
    39241 tttgatatgc gatatcttcg aatgttaagt tgagcgcctt taaaacttca cttttagtaa
    39301 tatcatgatt caacctttga tcaatcaact taatgaagaa acggcgaact tcaaattcat
    39361 caccaacaat ttcataacca tgctttcgag aatacttaag tgacaaacca tgatcttcca
    39421 attgctcttt cacatgattt atatcgtgaa tgacagtatt tttactgact tgtaaatcaa
    39481 ttgaaaaatg gtttagagac attgcatttt ccttactaaa aagcatgagc attaaataat
    39541 aacgacgtgt ttctatgcta aaaatgacat tgttgccgtt taacatttgc tgctccgata
    39601 catctcgctt gaataacgtc atgatttcag aacttacaat aaaatttcct tggcttgttc
    39661 tttcaagttt tggataaccc tcttgttcaa gccacaaatt gattttttga atgcgatatc
    39721 ctagttgtct acgagacaaa ccaaatatcg attcaagttc tttaccatga atagtaggat
    39781 tcaatacaat ttctctgagt atttgctgat tatccccgtt catgttcgat cctcctttga
    39841 gcatctgagc tcaatttatc atcaatcgtc acaccgatgt acacactttg aacacatatt
    39901 tccaaaatga gcatgtacat cattgtgatg ttttaacaac atttcaatta tatctatatt
    39961 ttttgtgatt ttaatctttt aaaataaagc aattgaaatt tttacatata tttttgtgtt
    40021 ttatgttttt tgaagcattt ttaacataca tatctcaatc attatcaaat tgtcatgacc
    40081 aatgtaatcc actataaaaa ccctaaggac gcttatatca ggcgccttag ggttaactgt
    40141 atctatttaa ttaagtatta ttattcgtat gtacgtaact tatggtctat caagttccac
    40201 acttcttcaa catcaactgc tgtagcaaaa taagcattgg caggcttacc tgtaacatga
    40261 tttaaatcga cagccatagt gccataagtt agtggacttt gatgttcaat gtcgatatta
    40321 acgggtacca ttgtaaacaa ttctggttgt aacaaataca aaattgtaca agcatcatgt
    40381 attggaccac catccatatt aaagtgagtc ttgtatgtct tcttaaagaa ttgcaataat
    40441 tctacgacga actgtgcaac aggattattg atactttcaa agcgttcaat cacgtgattg
    40501 tcggctaaaa cttgatgtgt tacatctaaa ccaaacacat ttatagtaat cccactttca
    40561 aaaacacgct tcgctgcttc agcatctacc caaatattga attctgctgt aggcgtccaa
    40621 tttccaaatg taccaccacc catcaaagta atagattcaa tatgctcagc gattcttggc
    40681 tcacgaatca atgccgttgc tactttcgta agaggacctg tcgctacaat tgttacaggt
    40741 gtatcactcg tcatcacttt gtttataatc acatctgatg ctggcattgc aactgcttga
    40801 cgtgatggtg tcgacggtag tttcggacca tctaatccag attccccatg tatttcagaa
    40861 gcaaaggcag ctggtttaat taacggccta tccgcacctt tcgctactgc tatatcttgg
    40921 cgtcccataa tatccaatac gttcaaggcg tttgtcgtat tcttgtcaac tgattgatta
    40981 cctgcgactg ttgttacagc taatatctct agtggactgt caattgcccc cgctaaaatt
    41041 aatgctattg catcatcgtg tcctggatca caatccataa taatctttct tttcatttat
    41101 atatccacct ttcttaagtt gttatcgata gcttatgtat atttatttat gtggtgaatc
    41161 atgtttattt tgaaaaatag ttttaacttt ctcatatttt tggatacaaa cactatttat
    41221 ctattttatg gcttataaat ttatccgata tgccttatca acctacctcg ctaaaaatag
    41281 gatgtctaca tatctatacc gacttttgtc aactcatttt cacaacaata taaacagcaa
    41341 tttatatgat tgttacatga ttcaaacaat ttttatgaaa aatattttca tacacagaat
    41401 atatattgat attaaatttc tcaaaagcta tattgagaat aattaggagg gatgttgatg
    41461 aaatctttat ttgaaaaagc acagcagttc ggcaagtcct ttatgttacc tatcgcaatc
    41521 ttaccagctg caggtctatt gttgggtatc ggtggtgcat taagtaatcc aaacaccgtt
    41581 aaagcatacc ctattttaga tattacctta ttacaaaata tttttacatt aatgtcagct
    41641 gcaggtagta ttgttttcca aaatttaccg gtcatctttg caattggtgt cgcaatcgga
    41701 ttatctagaa gcgataaagg tactgcaggt ttagctgcgc tgctcggttt cttaattatg
    41761 aacgcaacta tgaatggctt attaactatc acgggcacat tggcaaaaga tcagcttgca
    41821 caaaatggac aaggcatggt gctcggtata caaacggttg aaaccggtgt ttttggcggg
    41881 attatcacag gtattatgac cgcaatactt cacaacaaat atcacaaagt ggtattacca
    41941 ccgtatttag gtttctttgg tggctctaga tttgtcccta ttgtcacagc atttgccgca
    42001 atctttttag gtgtattgat gtttttcatt tggccaagca tacaagccgg catttatcat
    42061 gttggtggat ttgtaacgaa aacaggtgcc atcggtactt ttgtttatgg cttcatctta
    42121 agattgttag gtccactcgg tttacaccat attttttact taccgttttg gcagacggca
    42181 cttggtggta ctttagaagt caaagggcac ttagttcaag gtacgcagaa catcttcttt
    42241 gctcaacttg gtgatccaga tgtgacgaag tattattcag gtgtgtcacg ctttatgtca
    42301 ggccgtttta ttacgatgat gttcggctta tgtggtgccg cacttgcaat ttatcacaca
    42361 gctaaacctg aacataaaaa agttgtcggc ggtttaatgt tatccgctgc actcacttca
    42421 tttttaacag gtattaccga acctttagag tttagtttct tgtttgtcgc acctattctt
    42481 tatgtaatcc atgccttctt tgatggatta gcatttatga tggcagacat tttcaacatt
    42541 acaattggtc aaaccttcag tggaggcttt atcgatttct tactctttgg tgtgctacaa
    42601 ggtaatagta aaacaaacta cctatacgtc atacctattg gaattgtgtg gttctgtttg
    42661 tattacatcg ttttcagatt cttaattacg aaatttaatt tcaaaacacc tggtcgagaa
    42721 gataaagctg cagcacaaca agttgaggct actgaaagag cacaaactat tgttgctggt
    42781 ttgggaggca aagataacat tgaaatcgtt gactgttgtg caacgagact acgcgtcaca
    42841 cttaatcaaa atgacaaagt cgataaagta ttactcgaaa gtactggtgc caaaggtgta
    42901 atccagcaag gcactggtgt gcaagtaatt tatgggcctc acgttacagt tatcaaaaat
    42961 gaaattgaag aattgctcgg ggattaagac taaccgaaat atcaacagaa ctaatggcaa
    43021 cgatgtacga agtaagaagt gacatcgttg cttttatttt taatgttaca tttgaagcat
    43081 taagttcatc atacactgta gtgagcccgc aaatcgcctc tgctagacaa tcatcttaat
    43141 gctatgatta aagcttaagt gccagatttg aatttaattt caacaacgac tttcactaca
    43201 ttaaaaatag ggctactcga cacatatagt tgtatcaaat agccctttat acaatttttt
    43261 gggtaaggtt ttacaatttt tggaatggta tagattttat aaaaagttat ttaagttctt
    43321 ctgcttcagc cataatatct tttaatgttt tagctgaatg tgcgaacttg ctttgttctt
    43381 cgtcgtttaa tgggatttct acgacgttgc gaataccgtt tctattgatg actgctggaa
    43441 caccaatata aacatcttct tcatcatatt cgccttctaa taatgctgat acagtcaata
    43501 cggcatcttc atttctgaaa atcgcttcag taattctagc taatcccatt gcaacaccat
    43561 aataagtggc acctttagct tgaataatgt catatgctgc atcacgtgtt tgaacaaaaa
    43621 tttgttcaat ttgcgctttg ccctcaggac gttgttcaag taatgtcttc aaaggttgac
    43681 ccgcaatatt agcgtgtgac catactggta attcagtgtc accatgttca ccaataattt
    43741 gagcatcgac gctacgtggc gcaacatcga acgcttcgct taacaataat ctaaagcgtg
    43801 cagagtctaa aattgtacca gaacctataa cacgttcttt aggtaaacca gagaatttcc
    43861 atgttgcata cgctaaaata tcaacaggat ttgtagctac caagaaaata ccatcaaatt
    43921 ttgatgccat tacttcacca acaattgatt tgaatatttt caagttttta gatactaaat
    43981 ctaaacgtgt ttctccaggt ttttgtgcag caccagcaca gatgacaact agatccgcat
    44041 catgacaatc actgtattcg ccagctttca cacgaactgt tgttggagaa tatggtgtgg
    44101 catgttttaa atccataaca tctcctcgaa ctttttcagt gtctaaatca atgatgacta
    44161 attcatcaac aatgctttgg ttcactaatg aaaatgcgta gcttgaacct actgcaccat
    44221 tacctattaa tacaactttg ttccctttaa atttgttcat tacaaaaact cccttatgat
    44281 taattcacta acatacgtgt agcttcaaat atgttagttt aatgctgctt attgacgata
    44341 caaaagcaaa taaacatctc ttttattttc aacgcataac ttaaaaggtc atgtgtcatc
    44401 cgcttttaag tttgtgattt atttcacata taaaatgtaa catgcattaa gtactgggtc
    44461 aatattaaat tgtgatttat ttcacatttt attttaattt ttacaccttt ttaatttgta
    44521 tacgattaca tcttaggtgt ctttagtctt cgtacttcgc cagtgattat ttacactttc
    44581 acatttttat tatcatgttt acttttttct aagaaaacaa caatgttttt tgaattagtc
    44641 aaataaatgc gcgcaatcgt cggtgtgcaa acagacaatt gtacacaatg cttattgata
    44701 agtatttaaa aaattaaaaa tgtcatacaa ttatcaaatt tgccatttta tttatatttt
    44761 ctcaaaccaa ttaattgaat atcgaaattt ttagtagaat aatcaaaata gacagattaa
    44821 aggaggagta tcatgcttac agaacaagag aaagacatta tcaaacaaac ggtgccttta
    44881 cttaaagaga aagggacaga aattacgtca atcttttatc caaaaatgtt taaagcgcat
    44941 cctgaacttt taaacatgtt taatcaaacg aaccaaaaac gaggcatgca atcttcagca
    45001 ttagcacaag ctgtaatggc cgctgcggtt aatatcgata acttaagtgt tattaaacca
    45061 gtcattatgc cagtcgcata taaacactgt gcattacaag tttatgctga acattatcca
    45121 attgtggggg aaaatttatt aaaagcaatc caagacgtga caggtttaga agaacatgac
    45181 cctgtgattc aagcttgggc aaaagcatat ggtgttattg cggatgtgtt catccaaatt
    45241 gaaaaagaaa tttatgatca aatgatgtgg attggtttta aaccattcaa aattaccaat
    45301 attaaacaag aatctgaaga cattaaatca tttacagttg aaactgaaga atatgacttt
    45361 agtgaattta caccaggcca atacatcaca gttgatgttt ctagtgataa acttccatat
    45421 agagctaaac gtcactattc tatcgtatca ggtgaaaaaa accatttaac ttttggcgtt
    45481 aaacgtgatg tcacaacaga acatgaaggc gaagtttcaa caattttaca cgatgagatt
    45541 aaagaaggcg atatgattaa tttagctgcg cctgtaggtg gattcgtatt agagaatacg
    45601 actgaaccac aacttttctt aggttcaggt attggcgtca cacctttagt agcaatgtat
    45661 gaagctgcct ctgccaaagg tttagataca cagatggttc aagttgctga aaatgaacaa
    45721 catttacctt tcaaagacaa cttcaacagt atcgcaagcc attatgacaa cgctaaatta
    45781 tatacacact taaaagataa acaaggctat attggcactg aagaattaca agcattttta
    45841 gcaaataaac ctgaaattta tatctgtggt ggtacaaaat tcttacaatc tatgatcgaa
    45901 gcacttaaat ctttaaatta cgatatggat cgcgtacact acgaaacatt tattccaaga
    45961 ctaagcgttg cagtataact gagaaaagag gtttagtgct catatgaaac ttaatcaacg
    46021 ttacgtaaaa gtatttgcat tatatttcgt aagtattgtt actgcaaata ttattgttaa
    46081 aaataataat ttaattaaaa cattgataca aaccatagcc gggtacacgg tctttgcagt
    46141 tggtttgaag tatttaacta aacgtaaaaa taaatgacat ctataccaaa acagctatga
    46201 ctttactttg tcatggctgt tttttaattg ggagtaggac agagatgata ttttcgcaaa
    46261 atttatttgg tcgtcccacc acaacatgca ttgatgtatg ctcactgaat ttcataagaa
    46321 aggagttcac aagatgaccg tagatattgg acggatttat gacaataaag ataataccga
    46381 cgctattcgt atcctagtcg atagagtctg gccgagaggt atttcgaaaa gaactgctaa
    46441 cctagattat tggttaaaag acattgcccc ttctactgag ttgcgacaat ggttccaaca
    46501 tgatcctaaa ctttttggag cttttaaaga aaaatatgaa aaagaattac gtgatcagga
    46561 tgcgcaaaaa gatgcttttg aaaaattaaa ggatattgta aatcagcata atcatgttct
    46621 attgttatat gcagcaaaag atactaaaca taaccaagct gtagtactac agcagttgct
    46681 caatacttag ttatatatgt ttatcgtgaa tcactataaa tgtcgctaac ttcatttcta
    46741 atatttattt ttaaaaagca taattactac aattaattga actttaataa ttactaaact
    46801 tgaacaacat tttactttaa aaaaataaag ttttaaaata ttaattgttg ggttttacat
    46861 agtaattaga aagagagtgt taatgaaatg aagtttaaaa gactagcaac tatattttca
    46921 gcagtattag tgttatcagg atgtggctca atgcattcat ctggcaaaga cttaaatatt
    46981 tcgttaccgt taaaaacaaa gtctattgca ccttatgaaa cagatgtacc agttaagatt
    47041 ggtgctgcag aatcattatt taaaacaaat gatcaaggaa aaatagaaaa agcattagtg
    47101 aaatcgtatc atcaaccaaa tgatacaacg ttagatatag aattaaaaga taatattaaa
    47161 tttcaaaacg gtcaaaaatt gactgcagaa aaagtgaaat ctagccttga aaatagcatg
    47221 aaaaaaagcg acttggtcaa atattcatta ccaatatcat caattaccgc taaaggtcaa
    47281 aaactgacaa ttaaaaccaa ctccgcttac cctgaacttg tatctgaatt agctaatcct
    47341 tttatggcaa tttattatac agatgctaaa tcagatgtta atcaaactcc tgttggtaca
    47401 ggcccttacc aaataaaaga ttataagcaa tctcgaaaaa tatcattgtc gaattttaag
    47461 gactattggc aaggtaaacc gaaacttgat catattactg tgacgtacca agaagacggc
    47521 aataatcgcg tcagaaattt agaatctcaa aaagatgatt taataactga tgtcccagtt
    47581 aataaagttc atgacataga aaataatcaa aatttaaaag tgtcaaaaga atctggattt
    47641 agaacttctt tacttatgta taatcatact aataaaaaaa tgactaaatc cgttcgtgaa
    47701 gcattagatc atatcattga tagacaaggt attgcagatc atatttatca aggttatgcg
    47761 aaacctgcaa cgagtccatt taatgataaa attccatata ttaaagaacc taagttaacc
    47821 aaacaaaata tcgaacaagc aaaaacgtta ttagctaaag atggttatac aaaagaacac
    47881 cctttaaaaa ttaagttaat tacgtatgac ggtcgtccag agctatcgaa aattgcgcaa
    47941 gtattacaat cagatgctaa aaaagcaaat attgaaatcg acattaaaag tgttgatgac
    48001 atagaaggtt acttaaaaga ccgttctgca tgggatgcaa cgatgtatag tttcggaaca
    48061 atccctcgcg gtgatacagg ttatttcttt aatcaagcat ataaaaaaga tggtgctatt
    48121 aataaaggag actacaataa tagcaacgtc gatgatttaa ttaatcaatt gaatcatact
    48181 gtagatgtta aggaacgtca caatatttct aatgatatta ttaaattatc aagtcgagat
    48241 gtaccaaaca gttatattgc ttacaacgat caaatagtcg ctgctaactc taaggttaaa
    48301 aattataaag taacacctga aggtatctat ttaatcgatt atcgaacaac gattgaacga
    48361 taagggagtg tgggacattg atttatgtct cagccctgct tcaactataa aaaccacgcc
    48421 gaagtcatcc aatatgatta tggcgtggtt taatttgctt atatgattgt attaatttct
    48481 gtttgatgaa tttcaataaa atgattatta catactttca atgaatcatg ttcattttat
    48541 ataaatctaa ttgtcactat ttgtttaacg tgaaacactt ttttaactcc aaaaagtatt
    48601 cctattccac tcttttaaag acaaattata aaaatagctt atgtcacaaa catatgagca
    48661 acataagctt taattattat tcgtctactt ttttctgttg ttctttaaat tctttctgca
    48721 gtaaattcac ttgttttaaa cctgccccca ttaaaatgaa tacaagtatc cacacaataa
    48781 cgattaaaat aaatattttc aacgtatcac tgccaagtac agttaaagat gtatcccaaa
    48841 tgccatgtaa accaacggct gataaaaaga atattaaaaa gcgtttatca aaaatatctt
    48901 tgaattcaaa gccatgctgt tctttcgcaa taactattgc agcaccaaca atcgctgacc
    48961 aaactaaatg accaccaatc gcagtccacg cacgtgtgaa gacaatatct aataatggga
    49021 cattttcccc taaagcgaaa ttcaaaatat aacctgctga ttcaaaaact gcgaaccctg
    49081 caccaatagc agcaccgatt aataatccat tcaaaatctt atttgttttc aatttattga
    49141 cgaaataaac aataatgagt gcttttccag tttcttcaac taatcctact aaaaatgcat
    49201 cgaaaaatgt taaagaacca aacctttcga attgatcact aaaaacgaca aatctatata
    49261 ataccatcgt acttagtaat gaaaatacgc cgccaataaa gaacatgata ataacttcaa
    49321 aaatgctaat atttttaaac gcatttgatt cataaaagaa gaacaaaccc gataatggta
    49381 ctgttaaagc ccctataaaa atgagacccg gaatcgcgtt agtgttatta aaaatcgctg
    49441 ccatgaccca taatccaata aatgttactg tgaaagcgat gaatactcga gaaaagagcc
    49501 atggcttccc ccattcttct gatatgtcac aaattgctgg cgtagtttta gcagtacccg
    49561 ctataaatat ttcatctgct tcgtttttag tatgcggctt aaatacttca gaaaacatat
    49621 cttttaaatt aagatttaag ctttcatcat tgccggctag tctaccgatt gatttggttg
    49681 cattatcaaa taaacttctt gcttcggttt ttaaatctgc attttgcgct gtttcatgcg
    49741 ctttggcttc catttgttta ttgatagatt cattatgtat gtcggacgtc ttagttgatt
    49801 gtgtacttgc tgatgacatc ccctcttcac ctgtattagg tgcaactgat tgcgtttgtg
    49861 ttgtctcttc tttttctgta tttcttcgat gattaagacc ttccatagac ttacctactt
    49921 tcacttttca taataaaact attttaattt aatattgatg g
//
LOCUS       DAOBBH010000002        40642 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.002, whole genome shotgun sequence.
ACCESSION   DAOBBH010000002 DAOBBH010000000
VERSION     DAOBBH010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 40642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40642
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.002"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(14..217)
                     /locus_tag="P6L01_000049"
     CDS             complement(14..217)
                     /locus_tag="P6L01_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029153.1"
                     /translation="MDKESVVASLARNKKIAVETMAGQRYIIERILHTNDEKHIHILK
                     PKDVVLDVDSIKEIDENHLNDAT"
     gene            396..908
                     /locus_tag="P6L01_000050"
     CDS             396..908
                     /locus_tag="P6L01_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HDP3029154.1"
                     /translation="MTLFLLEANNLDFASTKEELEAKAASLSTKTIPTLIEVQATENL
                     THGYFIVEANDEAEAKQFLTEADISIQLVKEVRLVGKDLDEVKNGDAHVDYLVTWNIP
                     EGITMDQYLARKKKNSVHYEEVPEVEFKRTYVCEDMSKCICLYNAPDEEAVRRARKAV
                     DTPIDGIEKL"
     gene            1250..1990
                     /locus_tag="P6L01_000051"
     CDS             1250..1990
                     /locus_tag="P6L01_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3029155.1"
                     /translation="MIKIQQLQHHFGSHKVIHNFNLDISKGEIVTFIGKSGCGKSTLL
                     NVIGGFIHPSSGRVIIDNEIKQQPSPDCLMLFQHHNLLPWKTINDNIRIGFQQKISDE
                     EINAQLKLVDLEDRGKHFPEQLSGGMKQRVALCRAHVHKPNVILMDEPLGALDAFTRY
                     KLQDQLVQLKHKTQSTIILVTHDIDEAIYLSDRIVLLGEGCNIISQYEITASHPRSRN
                     DSHLLKIRNEIMETFALNHHQVEPEYYL"
     gene            2004..2975
                     /locus_tag="P6L01_000052"
     CDS             2004..2975
                     /locus_tag="P6L01_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDP3029156.1"
                     /translation="MKRLSIIVIIGIFIITGCDWQRTSKEPSKNAQNQQVIKIGYLPI
                     THSANLMMTKKLLSQYNHPKYKLELVKFNNWPDLMDALNSGRIDGASTLIELAMKSKQ
                     KGSNIKAVALGHHEGNVIMGQKGMHLNEFNNGDDYHFGIPHRYSTHYLLLEELRKQLK
                     IKPGHFSYHEMSPAEMPAALSEHRITGYSVAEPFGALGEKLGKGKTLKHGDDVIPDAY
                     CCVLVLRGELLDQHKDVAQAFVQDYKKSGFKMNDRKQSVDIMTHHFKQSRDVLTQSAA
                     WTSYGDLTIKPSGYQEITTLVKQHHLFNPPAYDDFVEPSLYKEASRS"
     gene            2972..3733
                     /locus_tag="P6L01_000053"
     CDS             2972..3733
                     /locus_tag="P6L01_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3029157.1"
                     /translation="MTRPTNNKFILPIITFIIFLGIWEMVIIIGHYQPVLLPGPALVG
                     KSIWTFIVTGEIFQHLAISLWRFVAGFVVALLVAIPLGFLLGRNRWLYNAIEPLFQLI
                     RPISPIAWAPFVVLWFGIGSLPAIAIIFIAAFFPIVFNTIKGVRDIEPQYLKIAANLN
                     LTGWSLYRNILFPGAFKQIMAGIHMAVGTSWIFLVSGEMIGAQSGLGFLIVDARNMLN
                     LEDVLAAIFFIGLFGFIIDRFISYIEQFILRRFGE"
     gene            3746..4777
                     /locus_tag="P6L01_000054"
     CDS             3746..4777
                     /locus_tag="P6L01_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDP3029158.1"
                     /translation="MTLETLIKEQLDPHLVEVDEGTYYPRTFIQQLFVDGYFGEAALR
                     KNAEVIEAVSQSCLTTGFCLWCQLAFSTYLENATQPHLNNDLQQQLLSGEILGATGLS
                     NPMKSFNDLEKLNLEHTYVDGQLVVSGRMPAVSNIQEDHYFGAISKHESSDEFVMFIL
                     RANQDGITLVEKTNFLGVNGSATYQITLNQVVVPQSQIITHDAKQFAATIRPQFIAYQ
                     IPIGLGSIKSSLELIDAFSNAQNGINQYLEYDVEAFKKRYRQLREEYYAILDDGNLTS
                     HLSELISLKKDIGYLLLDVNQASVVNGGSRAYTPYSPQVRKLKEGFFFAALTPTLRHL
                     GKLEAELKG"
     gene            4994..5353
                     /locus_tag="P6L01_000055"
     CDS             4994..5353
                     /locus_tag="P6L01_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HDP3029159.1"
                     /translation="MNTIDTHTKEQQFSNLVRSYRKEYVGKGPNSIRVSFKDNWAIAH
                     MTGVLSKVESFYLNDKRNESMLHYTRTEKIKQMYKEIDVNEMESLVGAKFVKLFTDID
                     LNDDEVISIFVFDKSIE"
     gene            5527..6548
                     /locus_tag="P6L01_000056"
                     /pseudo
     CDS             5527..6548
                     /locus_tag="P6L01_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
     gene            6934..8160
                     /locus_tag="P6L01_000057"
     CDS             6934..8160
                     /locus_tag="P6L01_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDP3029160.1"
                     /translation="MKRLSTTLKVRLISNFLQLIITTAFIPFIALYLTDMLSQSIVGI
                     YLVGLVVLKFPLSIISGYLIEIFPKKLLVLIYQATMVIMLVFMGVFGSHQLWQIIGFC
                     VAYAIFTIVWGLQFPVMDTLIMDAITEDVEHYIYKISYWMTNLSVAIGALLGGLMYGY
                     SMLLLFLIAACIFLIVLFILYIWLPQDRNQVKQSDDKRHASRYQKLQIMNIFRSYKLV
                     LKDRNYMLLISGFSIIMMGEFSISSYIAIRLKDQFETISIGSYDITGAKMLAILLMIN
                     TVVVILLTYSISKVVLKIDFKKALITGLLIYIVGYSGLTYLNQFGLLVVFMIIATVGE
                     IIYSPIVSEQRFKIIPKAKRGTYSAVNALGIHFSETLARLGIVLGVFLTSLQMGLYMF
                     IVLTIGASMLVAGVFG"
     gene            8631..15806
                     /locus_tag="P6L01_000058"
     CDS             8631..15806
                     /locus_tag="P6L01_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HDP3029161.1"
                     /translation="MIMGNLRFQQEYFRIYKNNTESTTHRNAYWVKLAKNVEATKMMY
                     ALSTIVQQHASIRHFFDVTTDDNLTMILHEFLPFIEIKQVPSSSANYDLEAFFKQELS
                     TYHFNDSPLFKVKLFQFADAAYILLDFHVSIFDDSQIDIFLDDLCNAYRGNTVINNTR
                     QHAHINRNDDKDNQDASHIALDSNYFRLENNSDIHIDSYFPIKHPFEQALYQTYLIDD
                     MTSIDMASLAVSVYLANHIMSQQHDVTLGIHVPSHLPNDLHGNIVPLTLTIDAKDVCQ
                     RFTTDFNKCVLQNMSQLQCAKSSLSLETIFHCYHHMMSCCNDVIEDVHQIHDAHTSLA
                     DIEIFPHQHGFKIIYNSAAYDLLSIETLSDLVRNIYLQITEENGNKRTTVDELNLMTE
                     RDIQLYDDINLSLPEIDDAQTVVTLFEQQVEATPNHVAVQFDGVFITYQTLNARANDL
                     AHRLRNQYGVEPNDRVAVIAEKSIEMIIAMIGVLKAGGAYVPIDPNYPSDRQEYILKD
                     ATPKVVITYQALYENGKQNINHIDLNKIAWKNIDNLSKCNTLEDHAYVIYTSGTTGNP
                     KGTLIPHRGIVRLVHRNHYVPLNEETTILLSGTIAFDAATFEIYGALLNGGKLIVAKK
                     EQLLNPIAVEQLINENDVNTMWLTSSLFNQIASERIEVLVPLKYLLIGGEVLNAKWVD
                     LLNQKPKHPQIINGYGPTENTTFTTTYNIPNKVPNRIPIGKPILGTHVYIMQGERRCG
                     VGIPGELCTSGFGLAAGYLNQPELTADKFIKDSNINQLMYRSGDIVRLLPDGNIDYLY
                     RKDKQVKIRGFRIELSEVEHALERIQGINKAVVIVQNHDQDQYIVAYYEAMHTLSHNK
                     IKSQLRMTLPEYMIPVNFMHIEQIPITINGKLDKKALPIMDYVDTDAYVAPSTDTEHL
                     LCQIFADILHVNQVGIHDNFFELGGHSLKATLVVNRIEASTGKRLQIGDLLQKPTVFE
                     LAQAIAKVQEQNYEVIPEAIVKDDYVLSSAQKRMYLLWKSNHKDTVYNVPFLWRLSSG
                     LNVAQLQQAVQHLIARHEILRTQYIVVDDEVRQRIVADVVADFEEVNTHFTDEQEIMR
                     QFVAPFNLEKPSQIRVRYIRSPLHAYLFIDTHHIINDGMSNIQLMNDLNALYQHKLLL
                     PLKLQYKDYSEWMSHRDMTKHRQYWLSQFKDEVPILSLPTDYVRPNIKTTNGAMMSFT
                     MNQQMRQLLQKYVEKHQITDFMFFMSVVMTLLSRYARKDDVVVGSVMSARMHKGTEQM
                     LGMFANTLVYRGQPSPDKMWTQFLQEVKEMSLEAYEHQEYPFECLVNDLDQSHDASRN
                     PLFDVMLVLQNNETNHAHFGHSKLTHIQPKSVTAKFDLSFIIEEDRDDYTINIEYNTD
                     LYHSETVRHMGNQCMIMIDYILKHQDTLQICDIPNGTEELLNWVNTHVNDRMLNVPGN
                     KSIISYFNEVVSRQGNHVALVMNDLTMTYETLRNYVDAIAHMLLSNGVGNGQRVALFT
                     ERSFEMIAAMLATVKVGASYIPIDIDFPNKRQGAILEDAKVTAVMSYGVEIETTLPVI
                     QLENAKGFVESKENEQYDDLHGNQLENTAMLDNEMYAIYTSGTTGMPKGVAIRQRNLL
                     NLVHAWSTELQLGDNEVFLQHANIVFDASVMEIYCCLLNGHTLVIPDREERVNPEQLQ
                     QLINKHRVTVASIPLQMCSIMEDFYIEKLITGGATSTASFVKYIEKHCGTYFNAYGPS
                     ESTVITSYWSHHCGDLIPETIPIGKPLSNIQVYIMSDGLLCGIGMPGELCIAGDSLAI
                     GYINRPELMADKWQNNPFGKGKLYHSGDLARYTSDGQIEFLGRIDKQVKVNGYRIELD
                     EIENVILAIRGISDCVVTVSHFDTHDILNAYYVGEQQVEQDLKQYLNDQLPKYMIPKT
                     ITHIDCMPLTTNDKVDTTRLPNPSPIQQSNKVYSEPSNEIEQTFVDVFGEVLKQNDVG
                     VDDDFFELGGNSLEAMLVVSHLKRFGHHISMQTLYQYKTVRQIVNYMYQNQQSLVALP
                     DNLSELQKIVMSRYNLGILEDSLSHRPLGNTLLTGATGFLGAYLIEALQGYSHRIYCF
                     IRADNEEIAWYKLMTNLNDYFSEETVEMMLSNIEVIVGDFECMDDVVLPENMDTIIHA
                     GARTDHFGDDDEFEKVNVQGTVDVIRLAQQHHARLIYVSTISVGTYFDIDTEDVTFSE
                     ADVYKGQLLTSPYTRSKFYSELKVLEAVNNGLDGRIVRVGNLTSPYNGRWHMRNIKTN
                     RFSMVMNDLLQLDCIGVSMAEMPVDFSFVDTTARQIVALAQVNTPQIIYHVLSPNKMP
                     VKSLLECVKRKEIELVSDESFNEILQKQDMYETIGLTSVDREQQLAMIDTTLTLKIMN
                     HISEKWPTITNNWLYHWAQYIKTIFNK"
     gene            15819..16463
                     /locus_tag="P6L01_000059"
     CDS             15819..16463
                     /locus_tag="P6L01_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDP3029162.1"
                     /translation="MTVFVMQLQSNLKSIEELISQSRWSYKKPRTVNYRYNQDKLMHR
                     LGDILVQYGIQHDTGLLPHEWHYHISPRGKADIVQHNRDGQPIYVSLSYSYPYIVCVV
                     DKEPVGIDIEKISQRLDWRTLVTCFSTNEAHQICSLNDFYQIWTQKESFTKLIGEGLI
                     KGLDIYDMTQSHFYQSREVKFKQFIFDQFMVQVCFLGEAPWGYKKVSVFQLLSS"
     gene            complement(16791..17270)
                     /locus_tag="P6L01_000060"
     CDS             complement(16791..17270)
                     /locus_tag="P6L01_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HDP3029163.1"
                     /translation="MGIFIYAGIIGGLLSGIVKLGWEVMFPPRTPERNATNPPQELLQ
                     QLGFSSEFTHQTYTFSNMELPWVSFIVHFSFSIVIAIIYCILVKKYAYLAIGQGAVFG
                     IAIWVLFHLIIMPIMHTVPAVWDQPYQEHLSEFFGHIVWMMTIELVRQHFVYRYKLN"
     gene            complement(17540..18304)
                     /gene="argB"
                     /locus_tag="P6L01_000061"
     CDS             complement(17540..18304)
                     /gene="argB"
                     /locus_tag="P6L01_000061"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDP3029164.1"
                     /translation="MKFIVIKIGGSTLSDMHPSIINNIKHLRSNNIYPIIVHGGGPFI
                     NEALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQHQCSAIGLCGLD
                     AQLFEITSFDQQYGYVGVPTALNKDALQYLCTKFVPIINSIGFNNHDGEFYNINADTL
                     AYFIASSLKAPIYVLSNIAGVLINDVVIPQLPLVDIHQYIEHGDIYGGMIPKVLDAKN
                     AIENGCPKVIIASGNKPNIIESIYNNDFVGTTILNS"
     gene            complement(18320..19561)
                     /gene="argJ"
                     /locus_tag="P6L01_000062"
     CDS             complement(18320..19561)
                     /gene="argJ"
                     /locus_tag="P6L01_000062"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HDP3029165.1"
                     /translation="MKHQETTSQQYNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFG
                     WIYSSSLASSAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGID
                     DARQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHIKDTNFATPGAFNEA
                     ILTTDTCTKHIAVSLKIDGKTVTIGGSAKGSGMIHPNMATMLAFITTDASIESNTLHQ
                     LLKSSTDHTFNMITVDGDTSTNDMVLVMANHQVEHQILSQDHPQWETFVDAFNFVCTF
                     LAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIIT
                     AIGYSGCEIDPKCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHEHVTIDVQLGLGNAA
                     ATAYGCDLSYDYVRINASYRT"
     gene            complement(19573..20604)
                     /locus_tag="P6L01_000063"
     CDS             complement(19573..20604)
                     /locus_tag="P6L01_000063"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDP3029166.1"
                     /translation="MIKVGIVGGSGYGAIELIRLLQTHPHVTIAHIYSHSKVDEPLKL
                     TFPHLQHIMQHFEALTVDNNDCDVIFFATPAPVSKTCIPPLVEKGIHVIDLSGAFRIK
                     NREIYEAYYKETAAAQDDLNHAIYSISEWQSFDNNGTKLISNPGCFPTATLLALHPLI
                     SEKIVDLSSIIIDAKTGVSGAGRSLSQRVHFSEMNENLSAYAIGNHKHKPEIEQYLSI
                     IAGQDVSVIFTPHLVPMTRGILSTIYVKFSSEYTTESLHKLMTSYYANQPFVRIRDIG
                     TFPTTKEVLGSNYCDIGIYVDETTQTAILVSVIDNLVKGASGQAIQNLNILYDFEVTT
                     GLNQSPVYP"
     gene            complement(20643..21827)
                     /gene="rocD"
                     /locus_tag="P6L01_000064"
     CDS             complement(20643..21827)
                     /gene="rocD"
                     /locus_tag="P6L01_000064"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HDP3029167.1"
                     /translation="MNSIIELTDYYSSNNYAPLKLVISKGKGVKVWDTDGKQYIDCIS
                     GFSVANQGHCHPTIVKAMTEQASKLSIISRVLYSDNLGKWEEKICHLAKKDKVLSLNS
                     GTEAVEAAIKIARKWGSEVKGITDGQVEIIAMNNNFHGRTLGSLSLSNHDAYKAGFHP
                     LLQGTTTVDFGDIEQLTQAISPNTAAIILEPIQGEGGVNIPPKGYIQAVRQLCDKHQI
                     LMIADEIQVGLGRTGKWFAMEWEQVVPDIYILGKALGGGLYPVSAVLANNDVMRVLTP
                     GTHGSTFGGNPLAIAISTAALDVLKDEQLVERSERLGSFLLKALLQLKHPSIKEIRGR
                     GLFIGIELNTDAAPFVDQLIQRGILCKDTHRTIIRLSPPLVIDKEEIHQIVAAFQDVF
                     KN"
     gene            complement(22086..23435)
                     /gene="brnQ"
                     /locus_tag="P6L01_000065"
     CDS             complement(22086..23435)
                     /gene="brnQ"
                     /locus_tag="P6L01_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDP3029168.1"
                     /translation="MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIA
                     NLGFLITAIGLPFLGIIAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPR
                     LATTSFEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLV
                     LLAIILLFAFIHPLGGISDAPISKQYQSHALFNGFLDGYNTLDALASLAFGIIIVATI
                     KKLGIENPTDIAKETIKSGTISIIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQIT
                     QYYLGNYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNYLAIATVVSFISFLF
                     ANVGLTKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKIIYQCTIFFTMIAALVDG
                     LKASPEFISSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIP"
     gene            complement(23714..24271)
                     /locus_tag="P6L01_000066"
     CDS             complement(23714..24271)
                     /locus_tag="P6L01_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HDP3029169.1"
                     /translation="MINFNKTALVLIDLQEGILKMDYAPHTAENVVQNANKLIDVFRK
                     NNGFIAFVRVNFYDGKDVLQPNAMISLPPKEGDDYSRFHHLLDKRDDDFVIDKRHFSA
                     FVGTDLDLQLRRRGIDTIVLGGVATHIGVDTTARDAYQLNYNQFFVTDMMSAQNETLH
                     QFPIDNVFPLMGQTITTNDFLNILN"
     gene            complement(24338..25978)
                     /locus_tag="P6L01_000067"
     CDS             complement(24338..25978)
                     /locus_tag="P6L01_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-keto acid decarboxylase family protein"
                     /protein_id="HDP3029170.1"
                     /translation="MKQRIGAYLIDAFHRAGVDKIFGVPGDFNLAFLDDIISNPNVDW
                     VGNTNELNASYAADGYARLNGLAALVTTFGVGELSAVNGIAGSYAERIPVIAITGAPT
                     RAVEQAGKYVHHSLGEGTFDDYRKMFAHITVAQGYITPENATTEIPRLINTAIAERRP
                     VHLHLPIDVAISEIEIPTPFEVTATKDTDASTYIELLATKLHQAKQPIIITGHEINSF
                     HLHQELEDFVNQTQIPVAQLSLGKGAFNEENPYYMGIYDGKIAEDKIRDYVDNSDLIL
                     NIGAKLTDSATAGFSYQFNIDDVVMLNHHNIKIDDVTNDEISLPSLLKQLSNISYTNS
                     ATFPAYHRPTSPDYTVGTEPLTQQTYFKMMQNFLKPNDVIIADQGTSFFGAYDLALYK
                     NNTFIGQPLWGSIGYTLPATLGSQLADKDRRNLLLIGDGSLQLTVQAISTMIRQHIKP
                     VLFVINNDGYTVERLIHGMYEPYNEIHMWDYKALPAVFGGKNVEIHDVESSKDLQDTF
                     NAINGHPDVMHFVEVKMSVEDAPKKLIDIAKAFSQQNK"
     gene            26171..26272
                     /locus_tag="P6L01_000068"
     CDS             26171..26272
                     /locus_tag="P6L01_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029171.1"
                     /translation="MMPQNTIAKKKQARHLMKVYAPVYSKHYLASNN"
     gene            complement(26251..28296)
                     /locus_tag="P6L01_000069"
     CDS             complement(26251..28296)
                     /locus_tag="P6L01_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HDP3029172.1"
                     /translation="MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAIQGEALQHY
                     LPFIQNGGVQNVAKLMTAAGSIIFENLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMN
                     KTMGDFLQVTPKNVTDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPS
                     YLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQSGLNAFSTGLLDSNTGVAVFLFGF
                     IKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQG
                     EFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPL
                     LFFIHAVLDGLSFLTLYLLDVHLGYTFSGGFIDYVLLGVLPNKTQWWLVIPVGLVYAV
                     IYYFVFRFLIVKLKYKTPGREDKQSQAVTASATELPYAVLEAMGGKANIKHLDACITR
                     LRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENP
                     TTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIK
                     PSQGEVRAPFNGKIQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQ
                     EVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK"
     gene            28881..29936
                     /locus_tag="P6L01_000070"
     CDS             28881..29936
                     /locus_tag="P6L01_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDP3029173.1"
                     /translation="MTGFSVYLGQPLDEAYIKRMIKQGYQMIFTSVQIPEEDDETKYH
                     YFTKLLNLLKYEQVTYLIDANPSILTPSFYEHLRQYDAQFMIRIDHSTSIEAIEAIMA
                     QGLKCCLNASIISRELLTSLHQQLNDFTLLSFCHNYYPRPDTGLSVDLVNKKNELIYQ
                     FNPKAQIYGFIVGSGLRGPLHKGLPTIEATRHSHPVVAAKLLQETGVSEVLVGDSLIE
                     MRQAKQLIDFCKHRHFTLCIEEVFDTTVTYLFDMCHKVRPDNPENVIRSETSRQICPH
                     SIQPQFTTQRRIGSVTVDNLNNGRYQGEMQIVRQTLSAHDNVNVVAQIIKEDLPLLSC
                     IEPNDTFDFQKTRECKK"
     gene            29936..30832
                     /gene="murQ"
                     /locus_tag="P6L01_000071"
     CDS             29936..30832
                     /gene="murQ"
                     /locus_tag="P6L01_000071"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDP3029174.1"
                     /translation="MENSTTEARNEATMHLDEMTVEEALITMNKEDQQVPLAVRKAIP
                     QLTKVIKKTIAQYKKGGRLIYIGAGTSGRLGVLDAAECVPTFNTDPHEIIGIIAGGQH
                     AMTMAVEGAEDHKKLAEEDLKNIDLTSKDVVIGIAASGKTPYVIGGLTFANTIGATTV
                     SISCNEHAVISEIAQYPVEVKVGPEVLTGSTRLKSGTAQKLILNMISTITMVGVGKVY
                     DNLMIDVKATNQKLIDRSVRIIQEICAITYDEAMALYQVSEHDVKVATVMGMCGISKE
                     EATRRLLNNGDIVKRAIRDRQP"
     gene            30844..32298
                     /locus_tag="P6L01_000072"
     CDS             30844..32298
                     /locus_tag="P6L01_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDP3029175.1"
                     /translation="MTKEQQLAERIIAAVGGMDNIDSVMNCMTRVRIKVLDENKVDDQ
                     ELRHIDGVMGVIHDERIQVVVGPGTVNKVANHMAELSGVKLGDPIPHHHNDSEKMDYK
                     SYAADKAKANKEAHKAKQKNGKLNKVLKSIANIFIPLIPAFIGAGLIGGIAAVLSNLM
                     VAGYISGAWITQLITVFNVIKDGMLAYLAIFTGINAAKEFGATPGLGGVIGGTTLLTG
                     IAGKNILMNVFTGEPLQPGQGGIIGVIFAVWILSIVEKRLHKIVPNAIDIIVTPTIAL
                     LIVGLLTIFIFMPLAGFVSDSLVSVVNGIISIGGVFSGFIIGASFLPLVMLGLHHIFT
                     PIHIEMINQSGATYLLPIAAMAGAGQVGAALALWVRCKRNTTLRNTLKGALPVGFLGI
                     GEPLIYGVTLPLGRPFLTACIGGGIGGAVIGGIGHIGAKAIGPSGVSLLPLISDNMYL
                     GYIAGLLAAYAGGFVCTYLFGTTKAMRQTDLLGD"
     gene            32301..33176
                     /locus_tag="P6L01_000073"
     CDS             32301..33176
                     /locus_tag="P6L01_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDP3029176.1"
                     /translation="MTNILYRIDKQLSDFTKTEKIIADYILKNPHKIIDMTVNDLADV
                     TNVSTASIVRFSRKMTHQGFQELKIAISRYLPEDIATNPHLELIENESVETLKNKMIA
                     RATNTMRFVATNIMDAQIDAICDVLKNARTIFLFGFGASSLTIGDLFQKLSRIGLNVR
                     LLHETHLLVSTFATHDDRDCMIFVTNQGSHSELQSIAQVATHYSIPIITISSTANNPV
                     AQIADYALIYGRTDENEMRMAATTSLFAQLFTVDILYYRFVALNYHAILDCITQSKMA
                     LDNYRKHLATIDFKH"
     gene            complement(33370..33651)
                     /locus_tag="P6L01_000074"
     CDS             complement(33370..33651)
                     /locus_tag="P6L01_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029177.1"
                     /translation="MVLITYQIILFFIISLSYYLTLNHYMAVTVGNFTSIFGMFAAIL
                     FMYYYLLYKSPEYNQRKRFKHFIHITNLIIIAFSTFVLVHLALKLFFSI"
     gene            33864..36653
                     /locus_tag="P6L01_000075"
     CDS             33864..36653
                     /locus_tag="P6L01_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDP3029178.1"
                     /translation="MAYQSEYALENEMMNQLEQLGYERVTIRDNKQLLDNFRTILNER
                     HADKLEGNPLTDKEFQRLLTMIDGKSIFESARILRDKLPLRRDDESEIYLAFLDKKSW
                     CKNKFQVTNQVSVEDTYKARYDVTILINGLPLVQVELKRRGIDINEAFNQVKRYRKQN
                     YTGLFRYIQMFIISNGVETRYFSNNDSELLKSHMFYWSDKQNNRINTLQSFAESFMRP
                     CQLAKMISRYMIINETDRILMAMRPYQVYAVEALIQQATETGNNGYVWHTTGSGKTLT
                     SFKASQILSQQDDIKKVIFLVDRKDLDSQTEEEFNKFAKGAVDKTFNTSQLVRQLNDK
                     SLPLIVTTIQKMAKAIQGNAHLLEQYKTNKVVFIIDECHRSQFGDMHRLVKQHFKNAQ
                     YFGFTGTPRFPENSSQDGRTTADIFGRCLHTYLIRDAIHDGNVLGFSVDYINTFKNKA
                     LKAEDNSMVEAIDTEEVWLADKRVELVTRHIINNHDKYTRNRQYSSIFTVQSIHALIK
                     YYETFKRLNKKLEQPLTIAGIFTFKPNEDDRDGEVPYHSREKLEIMISDYNKKFETNF
                     STDTTNEYFNHISKNVKKGVKDSKIDILIVVNMFLTGFDSKVLNTLYVDKNLMYHDLI
                     QAYSRTNRVEKESKPFGKIVNYRDLKKETDDALRVFSQTNDTDTILMRSYEEYKKEFM
                     DAYRELKMIVPTPHMVDDIQDEEELKRFVEAYRLLAKIILRLKAFDEFEFTIDEIGMD
                     EQENEDYKSKYLAVYDQVKRATAEKNKVSILNDIDFEIEMMRNDTINVNYIMNILRQI
                     DLEDKAEQRRNQEQIRRILDHADDPTLRLKRDLIREFIDNVVPSLNKDDDIDQEYVNF
                     ESIKKEAEFKGFAGERSIDEQALKTISNDYQYSGVVNPHHLKKMIGDLPLKEKRKARK
                     AIESFVAETTEKYGV"
     gene            complement(36800..37399)
                     /locus_tag="P6L01_000076"
     CDS             complement(36800..37399)
                     /locus_tag="P6L01_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029179.1"
                     /translation="MSLSKLPLFNHFANIRIQPKLIIKLFLTLIIIVTYTFVSGLSVD
                     YKKLLSEAGLSETSLAQIKIFSQISIFFSRVFSVLFTFLIILVISRIMRSDANTKSIF
                     SATLSYILFTNAISLIILTIQISAGLSLIDYSITSLNIFAKGNRILGTFDLQFFVQAY
                     ILTVMLIGTSKLSIRASTIWGIIYLILIITFSLISISIQ"
     gene            37525..38637
                     /locus_tag="P6L01_000077"
     CDS             37525..38637
                     /locus_tag="P6L01_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDP3029180.1"
                     /translation="MRRKRKWLIVICVLLVLITISQILKHFNYGTESKEAYNTYTVEK
                     ERQLDLQGKAYPREVKTYYKNNQVGTYLGVQVADGQTVKKGDMLINYKINSNKRQQLV
                     DKINDTQHIITEVYLNITKGLNNREIQKKLIEYKQTLYEAQQQLYQYDSQNRDSMYAS
                     FDGKIMIKNIDNDSDNQPILYLISKESHIKTSVTEFDLNKVKKGDKVNLSITSTGKTG
                     AGIIKQISELPISYEENLSLHENDALHLPESNNDGELANSKSISASPIFKSNNNSELS
                     KYGVIIDDLSLPIRAGYSLEIKVPLNAIKIPKSVLTKGNNVFIVNKNSIVEKRNIRIK
                     RINGDIIVEKGLKPGDKLIKQPKSTMNDGDKVEISS"
     gene            38634..39314
                     /locus_tag="P6L01_000078"
     CDS             38634..39314
                     /locus_tag="P6L01_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3029181.1"
                     /translation="MIELKNVYRTFKNGDKNIRVLKDINIHINAGEFIAIMGPSGSGK
                     STLINILGFIDREYHGEYLFEGSNYQTQSDNQLANIRNKTVGFVFQNFKLIHNNTILE
                     NISMPLVYTNMNTIERKNKVMTVLSAVGLDGKGDFTPSKLSGGQQQRVAIARAIINNP
                     KFIIADEPTGALDSDTSKIIMDIFTSLNRDNHTTIILVTHDRKVAEKADRIIHILDGR
                     IQHEEVIK"
     gene            39311..40489
                     /locus_tag="P6L01_000079"
     CDS             39311..40489
                     /locus_tag="P6L01_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3029182.1"
                     /translation="MRKVLNIFTIAFKSILKNKGRNIFTMIGIIIGISSVITIMSLGN
                     GFKKTAADQFSDAGAGKQEALISFTFKVDEKIKKYPFNQRDIELVNQVDGVLDAKLKE
                     NKEEGIEATITNVQKKSDIFIIKKQNLHSFKVGRGFDKEDNELRKKIVVINDQVAKTV
                     FNNNAIGKSLYIEGQGFEVIGITDKLYSDSSTVIMPENTFNYYMGHLHQGLPTLQIII
                     EDGYNKKTVVKKVESLLNKKGSGSVLGEYTYTDTEEIIKSIDKIFDSITYFVAAVAGI
                     SLFIAGIGVMNVMYISVAERTEEIAIRRAFGAKSRDIELQFLIESILICVTSGFIGLI
                     LGVVFATIIDVLTPDYIKSVVSLSSVIIAVSVSILIGLLFGWIPARAASKKELIDIIK
                     "
BASE COUNT        13848 a         6146 c         7706 g        12942 t
ORIGIN      
        1 cgtttgtttt ggattatgtt gcatcattta aatgattttc gtcaatttct ttgatgctat
       61 ctacatctaa cacgacatct ttaggtttca aaatatgaat atgtttttca tcatttgtat
      121 gtaaaatgcg ttctatgatg tatctttgac cggccattgt ttctacagca atctttttgt
      181 ttctagctaa acttgctacg acagattctt tatccataat gatagccccc tatatatatg
      241 tttatttact tataccctaa catgattttt atactctttg aaaatatatt ttacagaatt
      301 ttatctaaat atttaaaaaa atcgctcaat atccttgtaa tccgataaga attatagtaa
      361 tattttttca accattgtta taggaggtct tattaatgac attattttta ttagaagcta
      421 acaatcttga ttttgcatca acgaaagaag aactagaagc aaaggcagca tcactatcta
      481 cgaagacaat tccaacatta attgaagtac aagctactga aaatttaact catggttatt
      541 ttattgtgga agcaaatgac gaagcagaag ctaaacaatt tttaacagaa gcagatatta
      601 gtattcaatt agtcaaagaa gtacgcttag ttggtaaaga tttagatgaa gttaaaaatg
      661 gtgatgcaca tgttgattac cttgtaactt ggaacattcc ggaaggcatt acgatggatc
      721 aatatttagc acgtaaaaag aaaaattctg ttcattatga agaagtgcca gaagttgaat
      781 ttaaacgcac atatgtatgt gaagatatgt ctaaatgtat ttgtttatac aacgcacctg
      841 atgaagaagc ggtacgtcgc gcgcgaaaag cagttgatac accgattgat ggcatcgaaa
      901 aactttaata agacaacaag ttgatgagat atatgtatat aggtttgacg tggatttcga
      961 ttgcagttaa ttagaatagc tcaatgctat aaatgtaagt agttgatatg aagaaactaa
     1021 tgaactaaat gcaagtattt tctaaaacaa tcattttatt gaaatttagt agagctgaaa
     1081 ttaatataac gtcgttaatt gaataacgct tatgttataa gagcactcat accaaaccat
     1141 aatcatctat agatataaca attcacgata taagggctgt gtttggcata gccctttaga
     1201 tatacactta attcctatta aaatagtagg gattaaaagg gggcttgtca tgattaaaat
     1261 tcaacaatta caacatcact ttggatcaca taaagtaatt cataacttta atttggacat
     1321 tagcaaggga gaaatagtca ctttcatagg gaaaagtggt tgcggaaagt ctactttact
     1381 caatgttatc ggtggattta ttcatccatc gtctggtcgt gtcattattg ataacgaaat
     1441 taaacaacag ccatctccag attgtttaat gctatttcaa catcataatt tgctgccatg
     1501 gaaaacgatt aatgacaaca ttaggattgg atttcaacag aaaattagtg atgaagagat
     1561 taacgcacag cttaaattag ttgatttaga agacagggga aagcattttc ccgagcaact
     1621 gtccgggggt atgaaacaac gtgtggcact atgtcgagcg catgtgcata agcctaacgt
     1681 tatattgatg gatgagccat taggtgcatt agatgcattt acacgttata aacttcagga
     1741 tcaactagtg caactaaaac ataaaacgca atcaactatt attttagtga cgcatgacat
     1801 tgatgaagct atttatcttt ccgaccgcat tgttctgtta ggtgaagggt gcaatattat
     1861 ttctcaatat gaaattacag catcacatcc acgcagtcgt aatgatagcc acctacttaa
     1921 gattcgtaat gaaattatgg aaacatttgc attgaatcat catcaagttg aacctgaata
     1981 ttatttataa ggagtgagtg acgatgaaaa ggttaagcat aatcgtcatc attggaatct
     2041 ttataattac aggatgtgat tggcaaagga cgtctaaaga accgtctaaa aatgcccaaa
     2101 atcagcaagt gattaaaatt ggatatttgc cgattacaca ttcagctaat ttgatgatga
     2161 ctaaaaaatt attatcacaa tacaatcatc cgaaatataa actagaatta gttaaattca
     2221 ataattggcc agatttaatg gacgcattaa acagtggtcg tattgatggt gcatcaactt
     2281 taatagagct agcgatgaaa tcaaaacaga agggctcaaa tataaaggct gtggcattgg
     2341 gccatcatga aggcaatgtc attatgggac aaaaaggtat gcacttaaat gaatttaata
     2401 atggcgatga ttaccatttt ggtataccac atcgttattc aacacattat cttttacttg
     2461 aggaattacg taaacaatta aagattaaac cggggcattt tagctatcat gaaatgtcgc
     2521 cagcagaaat gccagccgca ttgagtgaac acagaattac agggtattct gtagccgaac
     2581 cattcggtgc actgggtgaa aagttaggca aaggtaagac tttgaaacat ggtgatgacg
     2641 ttatacctga tgcgtattgc tgtgtgctag tactgagagg ggaattgctt gatcaacaca
     2701 aggatgtagc gcaagcattt gtacaagatt ataaaaagtc tggctttaaa atgaatgatc
     2761 gcaagcaaag tgtagacatt atgacgcatc attttaaaca aagtcgtgac gttttaacac
     2821 agtcagcggc atggacatcc tatggtgatt taacaattaa gccatccggc tatcaagaaa
     2881 ttacgacatt ggtaaaacaa catcatttgt ttaatccacc tgcatatgat gactttgttg
     2941 aaccgtcatt gtataaggag gcatcgcgtt catgacacgt cccacaaata acaaatttat
     3001 attacctatt atcacattta ttattttctt aggcatttgg gaaatggtca ttattattgg
     3061 gcattaccaa cctgtattgt tacctggtcc tgctcttgta ggaaaaagta tatggacttt
     3121 cattgttact ggagaaattt tccaacattt agcaattagt ttatggagat ttgtagcggg
     3181 ctttgttgtc gcattgttgg ttgctattcc attgggcttc ttgcttggaa ggaatcgttg
     3241 gctatacaac gctatcgaac cgctatttca attgattaga ccgatatctc cgatagcatg
     3301 ggcaccattt gttgttctat ggtttggtat tggtagtttg ccagcgattg cgattatttt
     3361 tatcgctgct tttttcccaa ttgtgttcaa tactattaaa ggcgttagag acattgaacc
     3421 tcaatattta aaaatagcgg caaatttaaa tttaactggg tggtcattgt atcgcaatat
     3481 attatttccc ggggcattta aacaaatcat ggctgggata catatggcgg taggaacaag
     3541 ttggatattt ttagtttctg gtgaaatgat tggtgcacaa tcgggattag gttttttaat
     3601 cgttgatgca cgaaatatgt tgaacttaga agatgtttta gcagcaatat tctttatcgg
     3661 attatttggt tttattattg atcgattcat tagttatatt gagcagttta tacttagaag
     3721 atttggtgaa taaggagaga tgatgatgac tttagaaacg cttatcaaag aacaattaga
     3781 tcctcattta gtagaagttg atgaagggac gtattatccg agaacattta ttcaacaatt
     3841 atttgtagat ggttatttcg gtgaggcggc attgagaaaa aatgctgaag taatcgaagc
     3901 tgtatcgcag tcttgtttga caacaggatt ttgtttatgg tgccaattag ctttttcaac
     3961 gtatttagaa aatgccacgc agccacattt aaataatgac ttacaacagc aattgttatc
     4021 tggagaaata ttaggtgcta ccggattgtc taatccgatg aagtcattta atgatttaga
     4081 aaagttgaac cttgaacaca cttatgttga tggacaattg gttgtcagtg gacgtatgcc
     4141 agctgtaagt aatattcaag aagaccatta ttttggtgcg atttcgaaac atgaatcatc
     4201 agatgaattt gtcatgttca ttctacgtgc caatcaagat ggtatcactc tcgttgaaaa
     4261 aaccaatttt ttaggggtca acgggtcagc aacgtatcaa atcacattga atcaagtcgt
     4321 agtgccacaa tcacaaatca tcacgcatga tgcgaagcag tttgcggcaa ctattcgccc
     4381 gcaatttatt gcttaccaaa ttccaatagg attaggctca attaaaagtt ctttagagtt
     4441 aattgatgca ttttcaaatg cgcaaaacgg aataaatcaa tatttagagt atgatgttga
     4501 agcttttaaa aaacgttatc gtcaacttag agaggaatat tatgcaatat tagatgacgg
     4561 taacttaact tcacatttaa gtgaattaat atcattgaag aaggacattg gctatttatt
     4621 gttagatgta aatcaagctt ctgtcgtcaa tggtggttca agagcgtaca caccatattc
     4681 gccacaagtt cgcaagttaa aagaaggatt cttcttcgca gcattaacgc cgacattaag
     4741 acatttaggt aaacttgaag cagagttgaa ggggtaagtg tgataagctg attttttgtt
     4801 tagatgcgtt tgttgaaaca ttttttaaaa taatataaat cttagtttat aaacattttc
     4861 tgttaatttg ttatatcctt ttaactagga aaatatacat ttcgtaataa taataatcgt
     4921 tatcattgaa aaagtgttaa taaggtgtat aatgaaaatg tgaacaatta atgaacttct
     4981 tattttaaag aaggtgaata ctatagatac gcatactaaa gaacaacaat tctcgaatct
     5041 agtaagatct tatcgtaaag aatacgtggg taaaggaccc aatagtattc gagtgtcgtt
     5101 taaagataat tgggcgattg cacatatgac aggtgttttg agtaaagttg agagttttta
     5161 cctaaacgac aaacgcaatg aatcaatgct ccattataca cgcacagaga agattaaaca
     5221 gatgtataaa gaaatagatg taaatgagat ggaaagtctt gtaggcgcta agtttgtaaa
     5281 attatttaca gatattgatt tgaatgatga tgaagtcatt tcaatatttg ttttcgataa
     5341 gtcaatagaa taagtgttgc tggtgtaagg tacacggtgc tgtttgctaa cttcgctttg
     5401 aatttaacaa taattcaagg gggtggtatg tcaaacggtg ccgttttttt tgtcatattt
     5461 ttaaaacaag caacatgcaa cacgtacttt aaggaagtca aaatttatca tttaggagag
     5521 atggatatga aaatcgtagc attatttcca gaagcagtag aaggtcaaga aaatcaatta
     5581 cttaatacta aaaaagcaat aggattaaaa acatttttag aggaaagagg acatgagttc
     5641 attatattag cagataatgg tgaagactta gataaacatt taccagatat ggatgtgatt
     5701 attagtgcgc cattttatcc tgcatatatg actcgtgaac gtattgaaaa agcaccgaac
     5761 ttgaaattag caattacagc aggtgtagga tctgaccatg tagatttagc ggcagcaagt
     5821 gaacacaata ttggtgtcgt tgaagttaca ggaagtaata cagttagtgt ggcagaacat
     5881 gcggttatgg atttattaat acttcttaga aactatgaag aaggtcatcg tcaatcagta
     5941 gaaggtgaat ggaacttgtc tcaagtaggt aatcatgcgc atgaattaca acacaaaaca
     6001 attggtattt ttggatttgg tcgaattgga caacttgttg ctgaaagatt agcgccattt
     6061 aatgtaacat tacaacacta tgatccaatc aacaagacca taaattgtct aaatttgtaa
     6121 gctttgatga acttgtttca tcaagtgatg cgattacaat tcatgcacca ttaacaccag
     6181 aaactgataa cttatttgat aaagatgttt taagtcgtat gaaaaaacac agttatttag
     6241 tgaatactgc acgtggtaaa attgtaaatc gcgatgcgtt agttgaagcg ttagcatccg
     6301 agcatttaca aggatatgct ggtgatgttt ggtatccaca acctgcacct gctgatcatc
     6361 catggagaac aatgcctaga aatgctatga cggttcacta ttcaggtatg actttagaag
     6421 cacaaaaacg cattgaagat ggagttaaag atattttaga gcgtttcttc aatcatgaac
     6481 ctttccaaga taaagatatt attgttgcaa gtggtcgtat tgctagtaaa agttatacag
     6541 ctaaatagaa taaggatgct gggctagcga ttaacgcttt caattttata taaatgaatc
     6601 atataagcac tactgctgtt gtaaagatgg cagtagtttt tttatgatta catctaagta
     6661 tagtcacggc tatgttagga caatgattta acatttacgc acatatgtgt tcacttacgc
     6721 aattattgat aaatttcatt catttggaat aatatatagt catatagtgc taattttttt
     6781 gaggcattat atgcaagatg cattgtaaaa tcgatgcatt tgtttgttaa ataactttgt
     6841 ataactaaaa attctttgtt tcaacgtata atctaataat agattttata taaatggtag
     6901 tgtgtatata tgtggatagg ggtgtagtta ttaatgaaac gcttaagtac gactttgaaa
     6961 gtacgattga ttagcaattt tttacagcta attattacga cagcatttat accgtttata
     7021 gcactatatt taacagatat gttaagtcaa tcaattgtcg gtatatatct tgttggttta
     7081 gtggttctaa aatttccatt gtccattata tctggttacc ttattgagat ttttccgaaa
     7141 aagttgctag tacttattta tcaagcgacg atggtgataa tgcttgtgtt catgggcgta
     7201 tttgggtcac atcaattgtg gcaaattatt ggtttttgtg ttgcatatgc catatttaca
     7261 atcgtttggg gattacaatt tccagttatg gacacattaa ttatggatgc aattaccgaa
     7321 gacgtggaac attatattta caagattagc tattggatga cgaacttatc ggtagctatt
     7381 ggggcattgt taggtggctt gatgtatggc tacagtatgt tactactttt cttaatagca
     7441 gcttgtatat ttttaattgt actctttatt ttatatattt ggttacctca agaccgaaat
     7501 caagtaaagc aaagtgatga caagaggcat gcaagtcgtt atcaaaaatt acaaataatg
     7561 aatatatttc gcagttataa attagttttg aaagaccgta attatatgtt attgatttcg
     7621 gggttcagta tcatcatgat gggtgaattt tcaatctcct catatattgc tattagacta
     7681 aaggatcagt ttgaaacaat aagtataggt tcatatgata ttacaggtgc taagatgtta
     7741 gcaatcttgc taatgattaa tacggtcgtc gtcattttac tcacgtattc aatctcgaaa
     7801 gttgtattga aaatagattt taaaaaagct ttaatcactg gtttgctgat ttatattgtt
     7861 ggctatagtg gtctaaccta tcttaatcag tttggcttat tagttgtttt tatgataatt
     7921 gcgactgtag gtgagattat ttattcgcct atagtttcag aacaacgctt taaaattatt
     7981 cctaaagcta aaagaggaac atatagtgca gttaatgcat taggtattca tttttcagaa
     8041 acactagcta ggttagggat tgtgttgggt gttttcttaa cgtcattaca aatgggactg
     8101 tatatgttta tcgttttaac aattggtgct agcatgcttg ttgctggtgt attcgggtga
     8161 caaaaacaag tgaatacaaa ttgaatttat ttaaatattt attttatgca tctttttata
     8221 ttgaaaacct attaatttgg tcgtaattaa aatagagata aatttattaa gaatttgtta
     8281 aaatttaagg ataacaattg ttttatgcga gttgtttgtt taatataaac ttgcttcatg
     8341 gatattttgt aacaacaata ttgaatttgc aatgagatat gaacaacaga aagtgattaa
     8401 cgagttgtag tgtgctattt atcgaagatg tatgtaattt acattgaaaa atataccaat
     8461 tgtaacgatg atacaagatg tattttaggg tagaatatat cgctcatatg ttgcgaatgg
     8521 tgttttatat taatcattac ataacgagtt aattaacatt tcgtcggtta tcatatcatc
     8581 atgcaatttt agtaagtaat gagtattatg atatgaaaga aggacttttt atgattatgg
     8641 gtaatttgag atttcaacag gaatattttc gtatatacaa aaataataca gaatcaacga
     8701 cacaccgtaa tgcgtattgg gttaaacttg ctaaaaatgt tgaagctact aaaatgatgt
     8761 atgcattatc gacaattgtg caacaacatg catctataag acattttttt gatgttacta
     8821 ccgatgacaa tttaacaatg atacttcatg aatttctgcc ttttattgag ataaaacaag
     8881 ttccatcttc ttccgcaaac tatgatttag aagctttttt taagcaagaa ttaagtactt
     8941 accattttaa tgattcacct ttattcaaag ttaaattgtt tcagttcgct gatgctgcat
     9001 atatactatt agattttcat gtgtccattt tcgatgatag tcaaattgat atttttcttg
     9061 atgatttatg caatgcatat cgtggcaata ctgttattaa caatactcga cagcatgcac
     9121 atataaatag aaatgatgat aaagacaatc aagatgcatc gcatatagca ttagactcaa
     9181 actattttcg gttagagaat aactctgaca tccatattga tagttatttt ccaattaagc
     9241 atccatttga acaagcttta tatcaaacgt atttgattga tgatatgaca tcaatagata
     9301 tggcatcgtt ggctgttagt gtgtatttag ctaatcatat aatgagtcaa caacatgatg
     9361 tcacattagg tatacatgta ccatcacatt taccaaatga tttacacgga aatattgtgc
     9421 cgttaacgtt aacaatcgat gcaaaagatg tatgtcaacg ttttacaaca gattttaata
     9481 aatgtgtgtt gcaaaatatg tcgcaattac agtgcgcgaa gtcttcgctt tcactagaga
     9541 ctatttttca ttgttatcat catatgatgt cttgttgtaa tgatgttatt gaggatgtac
     9601 atcaaataca tgatgcacat acatctttag cggatattga aatttttcca catcaacacg
     9661 ggttcaaaat tatatataac agtgcagcat atgatttgct ctcaatcgag acgctgagtg
     9721 acttagttcg aaatatttat ttgcaaatta ctgaagaaaa tggaaataaa cgaacaactg
     9781 tagatgaact taatttgatg acagaacgtg atattcaatt atatgacgat atcaatttaa
     9841 gtttgcctga gatagatgat gcgcaaacag ttgttacctt atttgagcaa caagttgaag
     9901 caacgccgaa tcatgtcgct gtgcaatttg acggagtgtt tataacatat caaacattga
     9961 atgcacgcgc gaatgattta gcacaccgtt tgagaaacca gtatggtgtt gaacctaatg
    10021 atcgtgtcgc tgtcatagct gaaaaaagta ttgagatgat aatagcgatg ataggtgtgt
    10081 tgaaagctgg tggggcttac gtgccaattg atccgaacta tccaagtgat cgtcaggagt
    10141 acattttaaa agatgcaacg cctaaagttg taataacgta ccaagcttta tatgaaaatg
    10201 gtaaacaaaa tattaatcac attgatttga ataagatagc gtggaaaaat attgataatc
    10261 tttctaaatg taacacgtta gaagatcatg cttatgttat ttacacgtcg gggacaactg
    10321 gtaaccctaa agggacacta attccgcacc gaggtattgt tcgcttggtc catcgaaatc
    10381 attatgtacc attaaatgaa gagacgacaa ttttgttatc aggaactata gcctttgatg
    10441 ctgcaacatt tgaaatatat ggtgcattgc tcaatggtgg aaagctgatt gttgctaaaa
    10501 aagaacaatt attaaatcca atagcggtag aacaattaat caatgaaaat gacgttaata
    10561 ctatgtggtt aacctcctca ttatttaatc agattgctag tgaacgaata gaagtattgg
    10621 taccgttaaa gtatttatta attggtggag aagtattgaa tgctaagtgg gtggatttgc
    10681 ttaatcaaaa accgaagcat cctcaaatta ttaatggtta tggaccaact gaaaatacaa
    10741 catttacaac gacgtataat atacctaaca aagttccaaa tcgtattcct attggtaaac
    10801 cgattctggg tactcatgtt tatatcatgc aaggcgagcg tcggtgtggc gttggtattc
    10861 ctggagaatt atgtacaagt ggctttgggt tagctgcagg ttatttaaat cagccagaat
    10921 tgacagcaga taaatttatc aaagattcaa atataaatca gctgatgtat agaagtggtg
    10981 atatcgttcg tttgttaccc gatggcaaca tagattattt atatcgaaag gacaaacaag
    11041 ttaagattcg agggtttagg attgagttgt cagaggttga gcatgcgctc gagcgtatac
    11101 aaggtattaa taaagcagtt gttattgttc aaaatcatga tcaagatcag tatatcgttg
    11161 cttattatga agcgatgcat acattatcac ataataagat taaatcacaa ttacgtatga
    11221 ccttaccgga gtacatgata ccagttaatt tcatgcatat tgagcaaatt cctattacta
    11281 ttaatgggaa attagataag aaggcattgc ctatcatgga ctatgtcgat acggatgcct
    11341 atgtagcacc gagtacagat accgaacact tgctatgcca aatttttgca gatattttac
    11401 atgtgaatca agtaggtatt catgataatt tctttgaatt aggtggccat tcattaaaag
    11461 caacgttagt ggtgaatcgg atagaggcat ctactgggaa acgattacaa attggtgatt
    11521 tattacaaaa gccaactgta tttgaactag cacaagcgat tgctaaggtt caagaacaaa
    11581 actatgaagt gattccagaa gctatagtta aagatgatta tgtgctgagc tctgcacaaa
    11641 agcgtatgta tttattatgg aaatcaaacc ataaagatac ggtgtataac gtaccttttt
    11701 tatggcggtt atcatcagga cttaatgtag ctcaattgca acaagcagtt cagcatttga
    11761 tagcgcgaca tgagattcta cgaacacaat atattgttgt agatgatgag gttcgacaac
    11821 gtattgtggc agatgttgta gctgactttg aagaagttaa cacgcatttt acggatgaac
    11881 aagaaattat gcgccaattt gtggcacctt ttaatttgga aaagccaagt caaattagag
    11941 tgagatacat tagaagtccc ttacatgcat acctgtttat agatacacat catatcatta
    12001 atgacggtat gagtaatata caattaatga atgatcttaa cgcactttat caacataaat
    12061 tattgttacc acttaaattg caatataaag actatagtga gtggatgtcg catcgtgata
    12121 tgacgaaaca tagacaatat tggttatctc aattcaaaga tgaagtacct attttaagct
    12181 taccgacaga ctatgttaga ccaaatatta aaacgacaaa tggagcaatg atgtcattta
    12241 caatgaatca acaaatgaga cagctacttc aaaagtatgt agaaaagcat caaattactg
    12301 attttatgtt ctttatgagt gtggtcatga cgttgttaag cagatatgct cgaaaagatg
    12361 atgttgttgt cggtagtgtg atgagtgcgc gtatgcataa aggcacggag caaatgctag
    12421 gcatgtttgc taatacgttg gtatatagag ggcaaccgtc acctgataaa atgtggacac
    12481 agtttttaca agaggttaag gaaatgagtt tggaggcata cgagcatcaa gaatacccat
    12541 tcgaatgttt agtaaatgac ttagatcaat cacatgatgc ctcacggaat ccattatttg
    12601 atgtcatgtt agtactacaa aacaatgaaa cgaatcatgc tcattttggg catagtaaat
    12661 taacacacat tcaacccaaa tcagtgacgg cgaaatttga tttatctttc atcattgaag
    12721 aagatcgcga tgactataca atcaatatcg agtataatac cgatttatat cactcagaaa
    12781 cagttcgtca catgggtaat caatgtatga ttatgattga ttatattttg aagcatcaag
    12841 atacactaca aatttgtgat ataccaaacg gtacggagga acttctaaat tgggtcaata
    12901 cgcatgttaa cgatcgaatg cttaatgtcc cgggaaataa atctatcata agttacttta
    12961 atgaagttgt ctcacgacaa ggtaatcatg ttgcgctagt catgaatgat ttgacaatga
    13021 cgtatgaaac attacgcaac tatgtggatg ccattgcgca catgctccta tcaaatggtg
    13081 tgggcaatgg tcaacgggtt gccttgttta cagaacgtag ttttgaaatg attgcggcga
    13141 tgttggcgac agttaaagta ggtgcatctt atatacctat cgatattgat tttccgaata
    13201 aacgacaagg tgcaattttg gaggatgcta aagtaactgc agtcatgtct tacggcgttg
    13261 aaattgaaac gacattacca gtcattcaat tggaaaatgc taaaggcttt gttgaatcaa
    13321 aggaaaatga acaatatgat gatttacatg gcaatcaact tgaaaacaca gcgatgttag
    13381 ataatgagat gtatgctatt tacacatctg gtacgaccgg gatgcctaaa ggggttgcca
    13441 tacgacaacg aaatttgttg aatttagtgc atgcatggtc aactgaattg caattaggcg
    13501 acaatgaagt atttttgcaa catgcaaata ttgtttttga tgcatcagtt atggagattt
    13561 attgttgttt gttaaatggt catacgcttg tgattccaga tagagaggaa cgtgttaatc
    13621 cagaacagtt acaacaactc attaataagc atcgtgtgac ggttgcgtcg attccgttac
    13681 agatgtgtag tattatggaa gacttttata ttgaaaagtt gattacaggc ggggcaacta
    13741 gtacggcatc ctttgttaaa tatattgaga agcattgtgg cacgtatttc aatgcctatg
    13801 gaccatctga gtcaacagtc atcacatcgt attggtcaca tcattgtggt gatttgatac
    13861 ctgagacgat tccaattggc aaacccttat ctaacatcca agtgtatatt atgtcagatg
    13921 gtttgttatg cggtattggt atgccaggcg agttgtgtat tgcaggtgat agtttagcga
    13981 taggatatat taaccgtcca gaattaatgg ctgataaatg gcaaaataat ccatttggta
    14041 aaggaaagtt gtatcatagt ggtgatttag cacgttatac atctgatggt caaattgaat
    14101 ttttaggaag aatagataaa caagtgaaag ttaacgggta ccgtattgaa cttgatgaaa
    14161 ttgaaaatgt aatattagct attcgtggta tatctgattg tgttgtaaca gtaagtcact
    14221 ttgatacgca tgatatattg aatgcttatt atgtcggaga gcaacaagtg gagcaggatt
    14281 tgaagcaata tttaaatgat cagctgccta agtatatgat tcctaagact ataacgcata
    14341 tcgattgtat gccattaacc acgaatgaca aggtggatac tacgcgtttg ccaaatccat
    14401 cacctataca acagtctaat aaagtgtata gcgaaccctc taatgaaatt gagcagacat
    14461 ttgttgatgt atttggagag gtattgaaac aaaatgatgt cggtgttgac gatgatttct
    14521 ttgaacttgg tggtaactca ttagaggcga tgttagttgt ctcgcattta aaacgatttg
    14581 gccatcatat ttcaatgcag acattatacc aatataaaac cgtgcgacag attgttaatt
    14641 atatgtacca aaatcaacaa tcattagttg cattaccgga taatctttcg gaattacaaa
    14701 agattgttat gtctcgttat aacttgggta ttttagagga tagtctaagt catcgacctc
    14761 taggaaatac actattgact ggcgcgacag gttttttagg tgcttatctg attgaagcac
    14821 tacaaggata cagtcatcgc atttattgtt tcatacgtgc tgataatgag gaaatagcat
    14881 ggtataagtt gatgacgaat ttaaatgatt atttttcaga agagacggtt gaaatgatgt
    14941 tatcaaacat tgaagtcatt gttggtgatt tcgagtgtat ggatgatgtt gttttacctg
    15001 aaaacatgga tacgattatt catgcaggtg ctcgtacgga tcactttggt gatgatgatg
    15061 aatttgaaaa agtaaatgtt caaggtactg ttgatgtcat acgtttggca caacaacatc
    15121 atgcaaggtt aatatatgtg tctacgataa gtgtgggaac ttattttgat atagacacag
    15181 aagatgtgac attttcagaa gcggatgtct ataaagggca actactaaca tcaccatata
    15241 cacggagcaa attttatagt gaattaaaag tattagaagc tgtaaataat ggcttagatg
    15301 gtcggattgt acgtgttggt aatttgacga gtccttacaa tggaagatgg catatgagaa
    15361 atataaagac taaccgtttt tcaatggtaa tgaatgattt gttacaactg gattgtatcg
    15421 gggttagcat ggctgaaatg cctgtagatt tttcttttgt ggatacgact gcaagacaaa
    15481 ttgtcgcatt agcacaggtc aacacaccac aaatcattta ccatgtgcta tcacctaata
    15541 aaatgccggt gaaatctttg ttagaatgcg ttaagcgcaa agaaattgaa ctcgtcagcg
    15601 atgaatcatt taatgaaatt ttacagaaac aagacatgta cgaaacgatt ggattaacta
    15661 gtgttgaccg tgaacaacaa ctagcaatga tagatacaac attaacatta aaaataatga
    15721 atcacatcag tgaaaaatgg ccaacgataa ctaacaattg gctgtatcat tgggcacaat
    15781 atatcaaaac aatattcaat aagtaagtag ggaaagttat gacagtattt gtaatgcaat
    15841 tacagagtaa cttgaaaagt attgaagaat taatatcaca aagtcgttgg tcatataaaa
    15901 aaccgcgtac agtcaactat agatacaatc aagataaact catgcacaga ttgggagata
    15961 ttttagtgca atatggaatt caacatgaca caggtttatt accacatgaa tggcattatc
    16021 acatttcgcc acgaggtaag gcagatattg ttcaacacaa tcgtgatgga cagcccatct
    16081 atgtgagctt atcatatagt tatccttata tcgtgtgtgt tgtcgataaa gaaccagttg
    16141 gtatagatat cgaaaagata tcacaacgtt tagactggcg tacgttagtg acgtgtttct
    16201 ctacaaacga agcacatcaa atatgtagtt taaatgattt ttatcaaata tggacacaaa
    16261 aagaaagttt tacaaaattg attggtgaag gtttaatcaa aggattggac atttatgata
    16321 tgacacaatc acacttttat caatcacgtg aagtgaagtt caaacaattt atttttgatc
    16381 agtttatggt acaggtatgt ttcttaggag aggcaccctg gggttataaa aaagtgtctg
    16441 tatttcagtt attgagtagt taatatgacg tctgacagta tcattgcgca gtgattttaa
    16501 actatactgc ctaaatgttt actgttagat gtatttttat tggaaaaatg aaaggtgaat
    16561 acacgatggc atcattcaaa atcatgacga gattaatgta agtgataagg tgaaaatata
    16621 ctgagcaata ttgaaattgc caaaaataat atcttgttac gacgcatttg agactttaat
    16681 gctcacgtaa gttggctgga atcatatcat gtcagttaat aaaataacgt gagcatcaat
    16741 catcatactc aaacgatgat ttaaaactca cgttaatgtt agtcagtgta ttaatttaat
    16801 ttatagcgat agacaaaatg ttgtcgcact aattcaattg tcatcatcca gacgatatgg
    16861 ccaaagaatt ctgacagatg ctcttggtat ggttgatccc acacagcagg tacagtatgc
    16921 atgattggca taatgataag gtggaataat acccaaatag caataccaaa aacagcacct
    16981 tgtccaattg ctaagtaagc gtatttttta actaatatgc agtaaataat tgcaatgacg
    17041 atagaaaaac taaagtggac aataaagctt acccaaggca attccatatt tgaaaatgta
    17101 tatgtttgat gcgtaaactc actactaaat cctaattgtt gcaataactc ttgaggtggg
    17161 ttcgttgcat tacgttctgg tgtgcgaggt ggaaacatga cctcccaacc taattttaca
    17221 attccagata acaagccacc gataattcca gcataaatga atatacccat ttgtcgtttc
    17281 gtcaacttaa ggactcctct ctatttacta tcctcattat agtattttgt gaaaataatc
    17341 acaataaaaa gctttgcaaa acttaaaata ttttgataaa caaatttgta aaaggttatt
    17401 agttttttac atctcatatt gtatcgtact gtattcaaaa ttgttaacga gatgaacggt
    17461 aaataaatgt tttagcgcaa tggataatcg aactggaatc atctcgcgtg tcaaaacttg
    17521 ttaggcctta atttcatagt tatgaattaa ggattgttgt gccaacaaaa tcattattgt
    17581 aaatagattc aatgatattt ggcttgtttc ctgatgcaat gataacttta ggacagccat
    17641 tttcaatcgc atttttggca tctagcactt tgggaatcat acctccataa atatcaccat
    17701 gttcaatata ttgatgaata tcgactaatg gcaattgagg tataacaaca tcattgatga
    17761 gtacacctgc aatattactt aatacataaa taggcgcttt taatgatgat gcaataaaat
    17821 aggcaagcgt gtcagcatta atattgtaaa attctccatc atggttattg aaaccaatcg
    17881 aattgatgat aggtacaaat ttagtacata aatactgtaa agcatcctta tttaaagcgg
    17941 tcggaacacc gacatatcca tattgttgat caaaagatgt aatttcaaac agctgtgcat
    18001 ccaaaccaca taagcctatt gcagaacatt ggtgctggtt aaattgagct actaatgcag
    18061 tgttaacgtc tgcaatgagc gtgtgtttag taatggtcat ggttgcttta tcagtcactc
    18121 ttaggccatt aacaaagtgt ggctcgattt gctggtttga taatgcttca ttaataaatg
    18181 ggccaccgcc atgaacgata atggggtaga tgttgtttga tcgtaaatgc ttaatgttgt
    18241 taataattga tggatgcatg tcactaagtg tactgccacc aattttaatg acaataaatt
    18301 tcatctaacc aacaccacct tatgttcgat atgatgcgtt aatacgcaca taatcatagg
    18361 ataaatcaca accgtatgca gtcgctgcag cgttacctaa accaagttga acgtcaattg
    18421 tgacatgttc atgagttaat gtgttcgaca tagcttgctc atcaaatagt acagccatac
    18481 ctttatccac gacaggtatt tggttcagtt gaacatatgt gcatttagga tcaatttcac
    18541 atccgctata gccaatagct gtaatgattc gaccaaaatt ggcatcttcg ccaaaaatag
    18601 ctgattttac tagatttgaa cttacgatag ttttaccgat ttttcttgca tctgatattg
    18661 atttagcgcc tgacacattg acgctgatta actttgttgc gccttcgcca tctctggcta
    18721 tagctttagc taaaaatgta cagacaaaat tgaatgcatc aacaaatgtt tcccattgtg
    18781 gatggtcttg actaagtatt tggtgttcaa cttggtgatt tgccatgact aataccatgt
    18841 catttgtact tgtatcgcca tcaacagtaa tcatattaaa tgtatggtca gttgaagatt
    18901 ttaataattg atgaagtgta ttcgattcaa tcgatgcatc ggttgttata aaagcaagca
    18961 tggtagccat atttggatga atcatacctg aacctttggc gctaccacca attgtaacgg
    19021 ttttaccatc gatttttagt gatacagcga tatgttttgt acaggtatca gttgttaaaa
    19081 ttgcctcgtt aaacgcacct ggcgttgcaa aattagtatc cttaatatgt tcggtcccag
    19141 tcttaatttt atccataggc aaatattcac caatgacccc agttgaagca acagcaacat
    19201 gctcagatgg tatttgaagt tgttgagcaa cccatgtttg tgtttgtcgt gcatcatcta
    19261 tgccttgttg accggtacaa gaatttgcat tagctgaatt aacaacaagt gcttgtaatt
    19321 ttcctttaga cttttgtaaa gtgtcttcag tgacaataag tggtgcagct ttaaactgat
    19381 ttaaagtata tacggcagct gaacttgcca aagacgatga gtaaatccac ccaaagtctt
    19441 ttttgttagc gcgtaaaccg atgtgcatac caccagccgt gaagccttga ggtgtactga
    19501 tatcgccatg tttaataatt gaaaagttat attgttgtga tgtcgtttct tgatgtttca
    19561 ttctaacacc ccttatggat aaactggtga ttgatttagg ccagtcgtta cttcaaaatc
    19621 atataatata tttaaatttt gaatggcttg accacttgcg cctttgacaa ggttatcaat
    19681 cactgatact aaaattgctg tttgtgttgt ttcatctaca tagataccga tatcgcagta
    19741 gttactaccg agtacttctt ttgtggttgg aaaagtccca atatctctaa ttctgacaaa
    19801 tggctgatta gcataataag aggtcattaa tttatgtaat gattcagtcg tatattcaga
    19861 tgaaaatttg acataaattg ttgataaaat acctcgtgtc attggtacga gatgtggtgt
    19921 aaatatgact gatacatctt gacccgcaat gatagataaa tattgctcga tttccggttt
    19981 gtgtttatgg tttccgattg cataagcact tagattttca ttcatttctg aaaaatgaac
    20041 acgttgtgat aatgaacgac cagcacctga cacgccggtc ttagcatcaa taataataga
    20101 tgacaaatct actatttttt cgctaataag tggatgtaat gctaataatg ttgctgtagg
    20161 gaaacagcca ggattagaaa tgagcttcgt tccattgtta tcaaacgatt gccattctga
    20221 aatgctgtaa atagcatgat tcaaatcatc ttgtgctgca gcagtttctt tgtaatatgc
    20281 ttcatatatt tcacgattct taattctaaa tgcgccagat aaatcgataa catgaatacc
    20341 tttttctact aagggaggga tacatgtttt acttacgggt gctggtgtcg caaagaaaat
    20401 tacatcacag tcattattgt ccactgtaag tgcttcgaaa tgttgcataa tatgttgtaa
    20461 atgtggaaat gttaatttca acggttcatc tacttttgaa tgtgagtaga tgtgtgcaat
    20521 cgttacatga ggatgtgttt gtaacaatcg aattaattca attgcgccat aaccgctacc
    20581 gccaacgata cctactttaa tcatcatgaa tccctcctat gtcatatata aatgattaat
    20641 tgttaatttt taaaaacgtc ttgaaaagct gcaacaattt gatggatttc ctctttatca
    20701 ataactagag gtggagacaa tcgaatgata gtacgatgcg tgtctttgca taagattcca
    20761 cgttgaatca gttgatccac aaaaggtgca gcatctgtgt taagctctat gcctataaat
    20821 aaaccacgac ctctaatttc tttaatacta ggatgtttaa gttgtagcaa cgcttttaat
    20881 aaaaatgaac ctaagcgttc tgatcgttca accagttgtt catctttaag tacatcaagc
    20941 gctgccgtcg atattgcaat ggctaaaggg ttaccaccaa atgttgaacc atgtgtacct
    21001 ggtgttagaa cacgcatgac atcattattt gcaagtacag cagatacagg gtataagccg
    21061 ccacccaatg ccttacctaa aatataaatg tctggaacaa cttgttccca ttccatcgca
    21121 aaccatttcc cagttctacc aagaccaact tgaatttcat ctgcaatcat taatatttga
    21181 tgtttatcac atagttgacg cacagcttga atatatcctt tcggtggtat attaacgcca
    21241 ccttcacctt gaattggttc caaaataatt gctgctgtat tcggtgaaat agcttgtgtt
    21301 aattgttcaa tgtctccaaa atctacagtt gtagtgcctt gaagtagggg gtgaaatcct
    21361 gctttatatg cgtcgtggtt agatagtgat aatgagccaa gtgtacgacc gtgaaaattg
    21421 ttattcatag cgatgatttc aacttgtccg tcagtaatgc ctttaacttc agagccccat
    21481 tttctagcaa ttttaatggc tgcttcaaca gcttcagtac cagagttaag ggagagtact
    21541 ttgtctttct tagcaagatg acaaattttt tcttcccatt tcccgagatt gtcactataa
    21601 aggacacgtg aaatgataga caactttgaa gcttgttctg tcatcgcttt aacaattgtt
    21661 ggatgacaat ggccttggtt tgcaactgaa aaacccgaaa tgcaatctat atattgtttg
    21721 ccatcagtat cccaaacttt gacaccttta cctttagaaa tgacaagctt aagtggtgca
    21781 taattattag agctataata atcagttaat tcaatgattg aattcatcgg tctccctcct
    21841 ttatatttat acaatttaaa gaattaatat taatatatta cagttgttca atataagtgt
    21901 caaatgtaaa tttaataata taaagataag gttgtgtttg tattaatgtg atttgtaaaa
    21961 tgaaagtact aatatttttt gtggataatc agtgatatgt atgtatacga ttgcagttta
    22021 atgtagtatg aataaaaaat gccacaagca tatgtttaac atatgactgt ggcatagtat
    22081 tatgcttatg gaattttacg atgcttaatt ttataaataa tgaagccgat aatgaaacca
    22141 atcaaactga gaacaaccca gcccatacca atgtctgata atggtaaata tttttggctg
    22201 aaattaatca aagtttgtga gaatgatgtg cttgaaatga actctggact agcttttaat
    22261 ccatctacta atgcagcaat cattgtaaag aaaatggtac attgataaat aatttttgaa
    22321 tgatggaatt tgctactaaa taatgttagt acaatcaagg caattgctaa tggatataag
    22381 aacattaaca ctgggactga gtacataata atcttagtta agccaacatt cgcgaataag
    22441 aacgaaataa agcttacaac tgttgcaatc gctaggtaat tcattttagg gaaaaggtgt
    22501 tcgaatgttt ctgaaaatgc cgtaatcaaa ccgatggctg tttttaaaca agcaaccata
    22561 acgataagtg acaacaggac gataccgtag ttacctaagt agtattgcgt aatttgcgct
    22621 aaggcaatac caccattttc actaagtttg aaatgaccaa tacttaatgt acccatgatt
    22681 gctagtaggg tataaataat ccccatcata atgatactga tagtaccaga cttaattgtt
    22741 tctttagcga tatcagttgg attttcgata cctaactttt taatcgttgc aacaatgatg
    22801 ataccaaatg ccaatgacgc tagcgcatct aaggtattgt atccatctaa aaagccgtta
    22861 aataaggcat gtgattgata ttgtttacta ataggtgcat cagatatgcc acctaatgga
    22921 tggataaaag caaataataa aataattgct aataatacta agaataccgg atttaaaaat
    22981 ttaccgatat attctaaaat tcttgatggc tttctcgcaa aaaaccatgc aatcacaaag
    23041 aagacgaagc taaaaataaa taaatataaa gtgatttgct ttggtgataa aaatggcgaa
    23101 aatgcaattt caaatgatgt cgttgccagt ctaggtaagg cgaaaaatgg tccgataact
    23161 agatataagg caatcgtgaa aatgtaagca tatgttttat taacacgcga tgcaatttca
    23221 aataaaccag atgtctttga aatgccaata gcaatgatac ctagaaatgg taagccaatt
    23281 gctgtaatta aaaatcctaa gttagcgata aaaacgttag aaccagcagc ttgacccaag
    23341 tgtattggga agataagatt gccggcacca aagaataaac caaataacat agaacctata
    23401 aacatgtttt ctttaaatgt tagtttcttc ttcatatatg tatttgttct cctttttaga
    23461 acttataaaa atctacttaa ttaagataat atcaaaaatt aaacctaaag tgatacataa
    23521 ttttgtaata aatactaaat tttttgaaaa ttccggaata aatactgttt tttatgtagt
    23581 aggggcgtaa gttaagaacg atttaataga tataggctaa gtttatagat aggaggttag
    23641 acaacccgac gtattaatac tttgtaaaat aaagataccc ccttcaacat gatcgaaggg
    23701 ggtaagtgta tgtttagttc aatatattta gaaagtcgtt tgtagttatt gtttgtccca
    23761 ttaatgggaa tacattatct attggaaatt gatgtagcgt ttcgttttgt gcactcatca
    23821 tatctgtaac aaaaaactga ttgtagttta attgataggc atctcgcgct gtcgtatcta
    23881 cgccaatatg cgttgcgaca ccaccaagaa caatcgtatc aattcctcga cgtcgcaatt
    23941 gtaagtccaa atctgttcct acaaatgcac taaaatgtcg tttgtctatg acaaaatcgt
    24001 catctctctt gtctaataaa tgatggaaac gactgtagtc gtcgccttct tttggtggta
    24061 atgagatcat tgcatttggt tgcaatacat ctttaccatc atagaaattc acgcgaacaa
    24121 aagcgataaa gccattgttt tttctaaaaa catctattaa tttattagcg ttttgaacga
    24181 cattttcagc tgtatgtggc gcataatcca tcttaagtat accttcttgc aggtcgatta
    24241 acactaaagc ggttttatta aaattaatca tcttatatca gagtcctttt cgtttaataa
    24301 agtattcgct taaacttata ccctgtatac gatgaaatta tttattttgt tgtgaaaaag
    24361 ctttagcgat atcgatcagt ttcttcggtg cgtcttcgac agacattttg acttcgacaa
    24421 aatgcatcac atcgggatga ccattaattg cattaaacgt gtcttgtaaa tcttttgatg
    24481 attcaacgtc atgaatttca acatttttac caccaaatac agctggtaaa gctttataat
    24541 cccacatgtg aatttcatta taaggttcat acatgccgtg aataagtcgt tctaccgtat
    24601 agccgtcatt attaatcaca aataataccg gtttaatatg ctgtctaatc atagttgaaa
    24661 tagcttgaac agttagttgc aatgagccat caccaattaa taataagtta cgacgatctt
    24721 tgtctgctaa ttgtgaacct aatgttgcag gtaatgtata gccgatagaa ccccataacg
    24781 gttgccctat aaaagtattg tttttgtata atgctaaatc ataagcacca aagaatgatg
    24841 taccttgatc agcaataatg acatcatttg gttttaagaa attttgcatc attttaaaat
    24901 aagtttgttg tgttaatggt tctgtgccaa cagtataatc gggtgatgtt ggacgatgat
    24961 acgcagggaa cgttgcactg ttcgtatatg aaatattgga taactgtttt aacaacgatg
    25021 gtagagatat ttcatcattt gtaacatcat caattttgat attgtgatga tttaacataa
    25081 cgacatcatc gatattgaat tggtatgaaa atcctgctgt tgctgaatct gttaatttgg
    25141 ctccaatatt taaaattaaa tcgctgttgt ccacataatc tcgtatttta tcttcggcaa
    25201 ttttcccgtc gtaaataccc atataatatg gattttcctc attaaaagca ccttttccta
    25261 atgaaagttg tgctactggt atctgtgttt gatttacaaa atcttctaat tcttgatgga
    25321 ggtgaaaact gttaatttca tgtccagtaa tgatgatagg ctgcttcgct tgatgcagtt
    25381 tagttgctaa taactctata tatgttgatg catccgtatc tttagttgcc gtcacttcaa
    25441 atggtgtcgg tatctcaatt tcagagattg cgacatcgat tggtaaatgt aaatgaactg
    25501 ggcgtctttc ggcgattgct gtattaatta aacgtggtat ttcggttgtt gcattttcag
    25561 gtgtgatata accttgtgca acggttatat gtgcaaacat ttttcggtag tcgtcaaatg
    25621 taccttcacc aagtgagtga tgtacatatt taccggcttg ttcaacagca cgtgtcggcg
    25681 cacctgtaat cgcaatgaca ggtatgcgtt cagcatatga acctgcgata ccgttgacgg
    25741 cacttaattc gccaacacca aatgtagtaa ctaatgcagc gagtccatta agacgggcat
    25801 aaccgtccgc tgcgtaactt gcgtttaatt catttgtatt tcctacccaa tctacattgg
    25861 gattgctgat aatatcgtct agaaaagcga gattaaaatc accaggaaca ccaaaaattt
    25921 tatcgacgcc tgctcgatga aaagcgtcaa ttaagtaagc tccaatgcgt tgtttcataa
    25981 aaaaaccacc ttttctctat atttacactt aaaatgatag cctcgaatac agtgtaaggt
    26041 caaggaagtt gactttgttg tgaaaacgct aacaatagtg aacaattaat actttttagg
    26101 aaatgagtat gaaggggggc ttaaattgat gatcttaaaa ttcaaaattt aaggggagag
    26161 cgaactatca atgatgccgc aaaatacaat agcaaagaaa aaacaggcgc gtcaccttat
    26221 gaaggtatac gcacctgttt atagtaagca ttatttagct tcaaataatt gatcgccaaa
    26281 tgaaatgttg ccatgttcac cttgtttaaa atcaaggttt gtaatgtttc cttgtgtcac
    26341 gataataggc gtaatatcac tctttgcatg attgcggatg tagtctaaat caaagttgat
    26401 taataaatca ccttgtttaa cttcttgacc ttcctcaaca tgtaaagtaa agccttctcc
    26461 gtttaattta acagtgtcta aaccgatgtg gattaatagt tctaaaccac tatctgatac
    26521 aagaccaatt gcatgttttg ttgggaaaat catttgtatt ttaccgttga atggtgcacg
    26581 aacttcacct tgtgaaggtt taatagcgat accgtcaccc atcatttttt cgctgaacac
    26641 ttgatcaggc acttctgata aaggtgttac ttcaccagtt aatggtgcat gaacgatatg
    26701 gctcaattcg cttgttgcag atttatcttc tgcaacaaca acagtttcgt ctttatcgtc
    26761 ttccatagta gtagggtttt ctactacttg accattcata atctgttgca tttcatgttt
    26821 gatttggtca gatttaggac caaaaattgc ttgcatatta ttgccgactt ctaatacacc
    26881 agatgcgcct aaatctttca aaccaggaac atcaacttta gatttgtcgt taacttcaac
    26941 acgtagacgt gtgatacaag cgtctaaatg tttaatgttt gctttgccac ccatagcttc
    27001 taatactgca tatggtaatt cagttgctga agcagtaaca gcttgtgatt gcttatcttc
    27061 acgacctggt gttttgtatt ttaattttac aattaagaat cggaatacga agtagtaaat
    27121 aactgcgtat acaagaccta caggaatgac taaccaccat tgtgtcttat taggtagtac
    27181 gccaagtaaa acgtagtcga tgaaaccacc tgagaaagta taacctaagt gaacatccaa
    27241 taagtacaat gttaagaatg ataaaccatc aagtactgcg tgaataaaga ataataatgg
    27301 tgctacgaat aagaatgaga attctaaagg ttctgtaata ccagttaaga atgatgttaa
    27361 agcagcagaa cccattaaac ctgctactac tttcttattt tcaggtttag ctgtgtgata
    27421 aattgctaaa gctgctgcag gtaaaccgaa catcataaca gggaattcac cttgcatgaa
    27481 tttaccagct gtcaaatgtg cgccttcacg aatttgttcg ataaagatac gttggtcacc
    27541 gtgaataatt tcaccagctg catttttcca tgaaccaaac tcgaaccaga acggtgcgtg
    27601 gaaaatgtga tgtagaccga atggaattaa taaacgcttg atgaaaccaa ataagaatac
    27661 ggcaacacca gtatttgaat ctaataatcc tgtactgaat gcatttaatc ctgattgaat
    27721 cgttggccaa attaatgcca ttggaaatgc taaaataaat gatgttgtag ccatcataat
    27781 aggtacgaaa cgcttaccag cgaagaaacc taaataagat ggtaagttaa tgttatagaa
    27841 cttgttataa caccaagctg ccagggcccc gataataata ccgccgaaca cacctgtttg
    27901 taatgttgga atacctaaaa tgctagcgta accactcgct ggatcagtaa catttttagg
    27961 tgtaacttgt aaaaagtcgc ccattgtttt gttcatgatt atgtaaccga cgaatgctgc
    28021 gatagctgct acgccatcac cgccagctaa tccgattgcg acacctaatg cgaaaatcat
    28081 aggtaagttt tcaaaaataa tactaccggc agctgtcatt aatttagcga cattttgaac
    28141 gccaccattt tgtataaacg gcaagtagtg ttgtaatgct tcgccttgta tagctgtacc
    28201 gatagctaat aacagacccg ctgctggtaa aattgcaaca ggtaacatta gcgctttacc
    28261 aatacgttgc aattgaccga aaagtttctt cctcacttgt ccaacctcca aagttgtatg
    28321 taatatttaa gattcaataa aaaagacacg agcaaataga ctgtgttggt aaccatcaca
    28381 gcttaattta actcatgcct aatcttactt agtaacacgt tggtgtatgt aattaatgta
    28441 aaagaagcaa ttgactaagt agtatataaa actgatacgt tagtttatct agcttaccat
    28501 cacatcttat tgaatcttat ttgtttgcga ctcctatttt agcatggtga agtatgcgtt
    28561 ttcaatacaa ttattaaatt caatttcaaa tctcttaaat gttcacagat tattattaaa
    28621 acataatata agataatcct gaataaaatg tataaaagtt gttgtaataa tccagcagta
    28681 gcagctatta tcagcactta aaagaaagga taatctttga tggtgtaata attaacttgt
    28741 gacagaaata tgtaagatta ttacaatgaa caggctttaa ctgattaata ttgtaatgtg
    28801 ttttctctta tagtaaaatg taagcgatta cacaactcaa ctatattttc ctaataatta
    28861 tattgataag gagggctact ttgacaggct tttcagtgta tttaggacaa cctttagatg
    28921 aagcgtatat taagcgaatg attaaacaag gttaccaaat gatttttaca tctgtacaaa
    28981 taccagaaga agatgacgag acaaaatatc attatttcac aaaactactc aatttattaa
    29041 aatatgaaca agtgacttac ctcatagatg ctaatccatc tattttaaca ccatcttttt
    29101 atgagcatct tcgacaatat gatgcacaat ttatgattcg tatcgatcat agtacatcaa
    29161 ttgaggcaat cgaagcgata atggcacagg gtttaaagtg ctgtttgaat gcaagtatta
    29221 tttcccggga attgttaaca agcttacatc aacaattgaa tgattttaca ttactttcat
    29281 tttgtcataa ctattatcca agaccagata cgggattatc tgttgacttg gtcaataaga
    29341 aaaatgaact catttatcaa tttaatccaa aggcacaaat atatggtttt attgtaggga
    29401 gtggtttgcg aggtcctttg cataaaggct tgccaacaat tgaagcaacg agacatagtc
    29461 atcctgtcgt tgcagctaaa ttattacaag aaactggtgt atctgaagtg ttagttggag
    29521 actcattgat tgaaatgagg caggcaaaac aacttataga tttttgcaag cataggcatt
    29581 tcacgttatg tattgaagaa gtgtttgata cgacagtgac ttaccttttc gatatgtgtc
    29641 ataaagtacg cccggataat ccggaaaatg tcattcgttc ggaaacgtca agacaaatat
    29701 gtccacattc gattcaacca cagtttacga cgcaacgacg cattggttca gtaaccgttg
    29761 ataatttgaa taacggacgt tatcaaggcg aaatgcaaat tgtgagacaa acgcttagtg
    29821 cacatgacaa tgtgaatgtt gttgcacaaa ttattaaaga agacttacca ctgttaagtt
    29881 gtatcgagcc gaatgataca tttgattttc aaaaaactag ggagtgtaag aagtgatgga
    29941 aaatagtacg accgaagcgc gtaatgaagc gacgatgcat cttgatgaaa tgactgtgga
    30001 agaggcttta attacgatga ataaagaaga tcagcaagtc ccgttagcag ttcgaaaggc
    30061 aataccacaa ttgacaaaag taataaaaaa aacaattgca cagtataaaa agggtggacg
    30121 attgatctat atcggtgcag gtacaagtgg aagattgggt gtcttagatg cagcggagtg
    30181 tgtacctaca ttcaatactg accctcatga aattataggt attattgctg gtggacaaca
    30241 tgctatgacg atggctgtag aaggtgcgga agatcacaaa aaattagcgg aagaagattt
    30301 gaaaaatata gatttaacat caaaagatgt cgttatagga attgccgcga gtggcaaaac
    30361 gccatatgtt ataggcggtt taacatttgc taacacaatc ggtgctacaa cagtatctat
    30421 ttcatgcaat gaacatgcag ttataagtga aattgcgcag tatccagtag aagttaaagt
    30481 tggtccagaa gtattaactg gttcaacacg tttaaagtct ggtacagcac aaaagttaat
    30541 tttaaatatg atttcaacca tcacaatggt tggtgtcgga aaagtttacg ataacctcat
    30601 gattgatgtt aaagcaacca atcaaaaact gatcgaccgt tcagtgcgta ttattcaaga
    30661 aatatgtgct atcacatatg atgaagcaat ggcgttatat caggtatctg agcatgatgt
    30721 gaaagttgcg acagttatgg gtatgtgtgg catttctaag gaagaagcaa caagacggtt
    30781 attaaacaat ggtgacattg ttaaacgagc aatcagagat agacaacctt aggagggatt
    30841 taaatgacca aagaacaaca acttgcagaa cgaattattg ctgcagtagg tggtatggat
    30901 aatatagata gtgtcatgaa ctgcatgaca cgtgtgcgta ttaaagtatt agatgaaaat
    30961 aaagtagatg accaagaact aaggcatatt gatggtgtca tgggtgttat acacgatgaa
    31021 cgcattcaag ttgtggttgg acctggtaca gtcaataaag tggctaatca tatggcggaa
    31081 ttaagtggtg ttaaactagg tgacccaata ccacaccatc acaatgatag tgaaaaaatg
    31141 gactataaat catatgcagc tgataaagca aaggcgaata aagaagcaca taaagcaaaa
    31201 caaaagaatg gtaagttgaa taaagtattg aaatcaattg ccaatatctt tataccgttg
    31261 attcctgcat ttattggagc tggattaatt ggtggtattg cagcagtact gagtaactta
    31321 atggtggcag gctatatttc aggtgcttgg attacgcaac ttataacagt attcaatgtc
    31381 attaaagacg gtatgttagc atacttagct atttttactg gtattaatgc ggctaaagaa
    31441 tttggtgcaa caccaggact tggtggcgtg attggtggta caacgttatt aacgggtatt
    31501 gctggtaaaa atattttaat gaatgtcttc actggagaac cattgcaacc tggacaaggt
    31561 ggaattattg gcgttatttt tgccgtttgg attttaagta ttgtcgaaaa gagattgcat
    31621 aaaattgtgc caaatgcgat tgatattatt gtaacgccga ctattgcatt gttgattgta
    31681 ggactattaa ctatctttat ctttatgcca ttagcaggtt ttgtttcaga cagtttagtt
    31741 tcagtagtta acggaattat tagtattggt ggcgtattta gtggatttat cattggagca
    31801 agcttcctac cgttagttat gttagggctt catcatattt ttacgccaat tcatatagaa
    31861 atgattaacc aatctggtgc tacttactta ttgccaattg cagcgatggc tggtgctgga
    31921 caagtaggtg ccgcattagc actttgggta agatgtaaac gcaacacaac attacgtaat
    31981 actttaaaag gtgcattgcc agttggtttc ctaggtatcg gagaaccatt aatctatggt
    32041 gtgactttgc cattaggtcg acctttctta actgcttgta ttggtggtgg tattggtggc
    32101 gctgtaatag gtggaattgg acatattggt gccaaagcaa taggcccaag tggtgtgtca
    32161 ctcttaccat taatctcaga taatatgtat ttaggttata ttgcaggatt acttgctgca
    32221 tatgctggtg gattcgtttg tacatattta tttggaacga caaaggcgat gcgacagaca
    32281 gatttgttgg gtgattaatg atgacaaata ttttatatcg cattgataaa cagttgagtg
    32341 attttacgaa gacagaaaag ataatcgctg attacatttt aaagaatcca cataaaatca
    32401 ttgatatgac ggtgaatgat ttggcagatg ttacgaatgt tagtacagca tcaattgtta
    32461 gatttagtcg gaaaatgaca catcaaggtt ttcaagagct aaagattgcg atatctcgat
    32521 acttacccga agatattgca accaatccac atttagaatt gattgaaaat gaatctgtag
    32581 aaactttgaa aaataaaatg attgctagag caacgaatac gatgcgattt gtagctacta
    32641 atattatgga tgcgcaaatt gatgcaattt gtgatgtgtt gaaaaatgcc aggacaatat
    32701 ttttatttgg atttggcgca tcgagtttga ctattggtga tctttttcaa aagttatctc
    32761 gtattggctt aaatgtcagg ttattacatg aaacgcattt acttgtgtca acatttgcga
    32821 cgcatgatga tagagattgc atgatttttg tgacgaatca aggtagtcat agtgaattgc
    32881 agtcaattgc acaggtggcc acacattaca gtattcccat cataactata tctagtacag
    32941 ctaataatcc agtggctcaa attgcagact atgcattgat ttatggcaga actgatgaaa
    33001 atgaaatgcg tatggcggct acaacttcac tatttgcaca gttattcacg gtagatatat
    33061 tgtactatcg atttgtagca ttaaattatc atgcgattct agattgtata acccaatcga
    33121 aaatggcact tgataattac cggaagcatc ttgcgacgat agattttaaa cattagatat
    33181 tgtgagcgtt ctaatataaa gttagttgca acgtaaacaa ttgaagtttt tgatgtgatt
    33241 cagttgtttg atgaaaaatt gttgctacaa gtttgtaaaa ctggcataga ggctattttg
    33301 aaaatgataa aggcgtgaca aattaaagca aagttcaggt ttgagctttg cttttttcat
    33361 agatggaaat taaatgctga agaataattt taatgctaaa tgaactaata cgaaggtgct
    33421 aaaagcaatt attatcaaat tagtgatatg aatgaaatgt ttaaatcgtt tgcgttgatt
    33481 gtattcggga cttttataga gtaggtagta gtacataaag agtatggctg cgaacatgcc
    33541 gaatattgaa gtgaagttac ctacagtgac tgccatgtaa tgatttaaag ttaaatagta
    33601 acttagacta ataataaaaa ataaaatgat ttgataggta attaacacca ttcaagtccc
    33661 tccattaatc gtagacaaaa aatttatcaa tgatttgatt atttgtattc aaattttagt
    33721 atacgactta cctcaaagac aaatattaaa agataagaca ataaaattag aataaattta
    33781 cgtttgaaga taaaaaacaa acatttttca ttaaagttta tgatatattt agagcaatag
    33841 aaagtgtatg gaaggggata tgaatggcat accaaagtga atacgcatta gaaaatgaaa
    33901 tgatgaatca acttgaacaa ttgggttacg aaagagtaac gatacgtgat aataagcaat
    33961 tgcttgataa ttttagaacg attttaaatg agcgtcatgc ggacaaatta gaaggcaatc
    34021 ccttaacaga taaagaattt caacgtctgt taacgatgat tgatgggaaa agtattttcg
    34081 agagtgcccg tattttacgt gataaattac cacttagacg tgatgatgag tctgagattt
    34141 atttggcgtt tttagataag aaaagttggt gtaaaaataa gtttcaagtg acgaatcaag
    34201 tatctgtcga ggatacatat aaagcacgtt atgatgtaac gatattaatc aacggactac
    34261 cccttgtcca agttgaattg aaacgtcgag gtattgatat taatgaggcg tttaaccaag
    34321 taaaacgtta ccgcaaacaa aattacacag gcttattccg ctacatacaa atgtttatca
    34381 ttagtaatgg tgttgaaacg cgatactttt ctaataatga tagcgaacta ttgaagagtc
    34441 acatgtttta ttggagtgat aaacagaata accgaatcaa tacattacaa tcatttgctg
    34501 agtcgtttat gagaccctgt caattagcta agatgatatc gcgctatatg attattaatg
    34561 aaacagatag aatactgatg gcaatgcgtc cgtatcaagt gtatgcggta gaagcactta
    34621 ttcaacaagc gactgagaca gggaataatg gatatgtatg gcatacaact ggaagtggta
    34681 agacattgac ttcttttaaa gcgagtcaga ttttatcaca gcaagatgac attaagaaag
    34741 ttatcttttt ggttgaccgt aaagacttgg atagtcaaac agaagaggaa tttaataaat
    34801 ttgctaaggg tgctgtagac aaaactttta atacctcgca actggtacgc caactaaatg
    34861 ataaaagttt gccacttatt gtaacgacga ttcaaaaaat ggctaaagcg attcaaggga
    34921 atgcccattt attagaacag tataaaacga ataaagttgt atttattatt gatgagtgtc
    34981 atcgcagtca atttggtgac atgcatcgtc tagttaaaca acatttcaaa aatgcccaat
    35041 actttggatt cactggtacg ccacgttttc cagaaaatag tagtcaagat ggtagaacaa
    35101 ctgcagatat tttcggtaga tgcttacata cgtatttaat tagagatgcc attcatgatg
    35161 gtaatgtact tggtttctca gttgactata ttaatacttt taaaaataaa gctttaaaag
    35221 cagaagataa cagcatggtt gaagcaattg atacggaaga agtatggtta gcggataaac
    35281 gtgtggaatt agtaacacga catatcatca ataatcatga taaatataca cgtaatcgtc
    35341 aatattcaag tatatttaca gtccaaagta ttcacgcgct tattaaatat tatgagacat
    35401 ttaagcgact taacaaaaag ttggaacaac cgttaacgat agctggtata tttacgttta
    35461 aacctaatga agatgatcgt gatggtgaag tgccatatca ttcacgtgaa aaattagaga
    35521 taatgattag tgattataat aaaaagttcg agacgaattt ttcaacagac acaactaatg
    35581 agtattttaa tcatatttca aaaaacgtta aaaagggcgt taaagatagt aaaattgata
    35641 tcttaatcgt tgttaatatg ttcttaactg gttttgatag taaagtactg aacactttat
    35701 atgttgataa gaatttaatg tatcatgatt taattcaagc gtattcacgt acaaataggg
    35761 ttgaaaaaga atcaaagcca tttggtaaaa ttgtaaacta tcgtgacttg aaaaaagaga
    35821 cagacgatgc actgagagta ttctcacaaa caaatgatac ggatacaatt ttaatgcgca
    35881 gttatgaaga gtataaaaaa gaatttatgg acgcttatcg tgagcttaaa atgattgtgc
    35941 cgacaccaca catggttgat gacattcaag atgaagaaga gctaaagcgc tttgttgaag
    36001 cttatcgttt attagctaaa ataatattac gtttaaaagc atttgacgag tttgagttta
    36061 caattgatga aattggaatg gatgaacaag agaatgaaga ctataaaagt aaatatttag
    36121 ctgtgtacga tcaagtaaaa agagcgacgg ctgagaaaaa taaagtatcc attttaaatg
    36181 atattgattt cgaaatagaa atgatgcgta atgatacgat taatgtgaat tatattatga
    36241 atatattgag acaaattgat cttgaagaca aagcggaaca acgtcgtaac caagaacaaa
    36301 ttagacgcat tttagatcat gcagatgatc cgacattgag gttaaaacga gatctaatta
    36361 gagaattcat cgacaatgtt gtaccttctt taaataagga tgatgatatc gatcaagaat
    36421 atgttaattt cgaaagtatt aaaaaagaag cggagttcaa aggatttgct ggagagagat
    36481 ctatcgatga acaagcccta aaaacaattt caaatgacta ccagtatagt ggtgttgtaa
    36541 acccacatca ccttaaaaaa atgattggtg atttgccatt gaaagaaaag cgtaaagcaa
    36601 gaaaagccat tgaatctttc gtggcagaaa caactgaaaa atacggtgtg taatgattca
    36661 gccccctcgc tagattagtg tagggggcat tattatttca ttagaaaagg ctcatttgtt
    36721 aaattcatct tatcaagaat ataacgaatg ttccttgttt taaacttatt aattaataga
    36781 gacattttat ctgaaaatat cactgaatgc taatactaat caaagaaaaa gtgataatta
    36841 aaataagata tataataccc caaatagtgg atgctcgtat agaaagtttc gacgtaccaa
    36901 ttagcatgac tgtaagtatg tatgcttgta caaaaaattg taagtcgaaa gtacccagaa
    36961 tgcgatttcc tttagcaaaa atatttaaac tagtaataga atagtcaatt aatgataaac
    37021 cggcacttat ttggatggta agtataatta aactaatagc atttgtaaac agtatgtagc
    37081 ttaacgttgc agagaatata gattttgtat tagcatctga gcgcataatt ctagagataa
    37141 ctagaatgat taaaaatgta aataatacgc tgaaaactct tgagaaaaaa atactaattt
    37201 gactaaaaat ttttatttgt gctaatgatg tctctgagag tcctgcttca ctcaataact
    37261 ttttatagtc cacagatagt ccactaacaa atgtatatgt aacaattatt atcaaagtca
    37321 gaaacagctt aatgattaac tttggttgaa tgcggatatt tgcaaagtga ttaaacaaag
    37381 gtagttttga taatgacata tagacctcct aatgatataa ttctcctata ttttatcata
    37441 aaaataatat aaaaaatata ttttattaaa actaagtata aaaaatggtt tgaagagtat
    37501 ttaacattta taggaggaca taaattgagg cgtaaaagga aatggctaat tgttatttgt
    37561 gtattattag tattgattac gatatcacaa attttgaaac attttaacta tggaactgag
    37621 agtaaagaag catacaatac atatacggtt gaaaaagaaa gacaattaga tttacaaggt
    37681 aaggcatatc cacgtgaagt taaaacatat tataaaaata atcaggttgg aacatatttg
    37741 ggtgtgcaag tagcggacgg gcaaacagtc aaaaaaggag acatgttaat taattataaa
    37801 ataaatagta ataaacgaca gcaacttgtt gataagataa atgatactca acatattatt
    37861 acagaagttt atttaaacat tactaaaggt ttaaacaatc gagagataca gaagaagtta
    37921 attgaatata agcagacatt atatgaagct caacagcaat tgtatcagta tgatagtcaa
    37981 aatagggata gtatgtatgc atcatttgat ggcaagatta tgatcaagaa tatagataat
    38041 gacagtgata atcaacctat actgtatctc atttcaaagg agtctcatat taaaacgtct
    38101 gttacggagt ttgatttaaa caaagtcaaa aaaggagaca aagttaactt aagtattacg
    38161 agtacgggta aaacaggagc ggggattata aaacaaatct ctgagttgcc tattagttat
    38221 gaagagaatt taagtcttca cgaaaatgat gccctgcatt taccagaaag taacaacgat
    38281 ggggaattag caaattcaaa atcgatttcg gcatctccaa tatttaagag taataacaac
    38341 tctgaattat caaagtatgg agttataatt gatgatctta gtctaccaat acgtgcaggt
    38401 tattcgttgg aaattaaagt cccattaaat gcgattaaaa tacctaaatc agtattaaca
    38461 aaaggcaata atgtttttat tgttaataaa aatagtattg tggaaaaacg gaatattcgt
    38521 ataaaaagaa tcaatggtga cattattgta gaaaaggggt taaaacctgg agacaagtta
    38581 attaaacaac ctaaaagtac aatgaatgat ggtgataagg ttgagatttc gtcatgatag
    38641 aattaaagaa tgtttatagg acttttaaaa acggtgataa aaatattcga gttttaaaag
    38701 acattaatat acatatcaat gcaggtgaat tcatagcgat tatgggacca tcaggctcag
    38761 ggaagagcac actgattaat atcttaggat ttatagatcg agagtatcat ggtgaatatt
    38821 tgtttgaagg aagtaattat cagacacaat ctgataatca attggcaaac ataagaaata
    38881 aaacagttgg atttgttttt caaaatttta aactaattca taataatact attttagaaa
    38941 atataagtat gccactggta tatacaaata tgaatacgat agaacgaaag aataaagtta
    39001 tgactgtgct cagtgctgta ggcctagatg gcaaaggtga ttttactcct agtaaactat
    39061 caggcggtca acaacaacgt gttgcaattg cgcgagccat tataaataat ccaaagttta
    39121 ttattgcaga tgagccaacg ggtgctttgg attcagatac atctaaaatt attatggaca
    39181 tatttacatc attaaaccga gataatcata ctacaattat tttagtcaca catgatcgta
    39241 aagtagcaga aaaagctgat cgaataattc atattttgga tggtcgtata caacatgaag
    39301 aggtgattaa atgaggaaag ttttaaatat ttttacaata gcttttaaat caatattgaa
    39361 aaataaaggt agaaatatat ttacaatgat aggtataatc atcggtattt cttctgttat
    39421 tacgataatg tctttgggaa atggctttaa aaaaactgct gctgatcaat tttcagatgc
    39481 tggcgccgga aaacaagaag cgttgattag ctttactttt aaagttgatg agaaaatcaa
    39541 aaagtatcct tttaatcaaa gagatataga gttggtaaat caagttgatg gcgtactaga
    39601 tgcgaagcta aaggaaaata aagaagaagg aattgaagcc acaataacta atgtccagaa
    39661 aaaaagtgac atcttcatta taaaaaaaca aaatttacac tcttttaaag taggtagagg
    39721 gtttgataaa gaggataatg aattacgtaa gaaaatagtg gttataaatg atcaagtagc
    39781 aaaaactgta ttcaataata atgctattgg taaatcgcta tatattgagg gacagggatt
    39841 tgaagttata ggaattacgg ataaactata ttctgactca tctaccgtta taatgcctga
    39901 aaatacattc aactactata tgggacactt acatcaaggt ctgcctacct tacagataat
    39961 tattgaagat ggttacaata aaaaaactgt agttaaaaaa gtagaatcac tattaaataa
    40021 aaaaggatcg ggctctgttt taggtgagta tacatataca gacactgaag aaattatcaa
    40081 aagtattgat aaaatttttg atagtattac ttattttgta gcagctgttg ccggtatttc
    40141 actttttatt gctggtattg gagttatgaa tgtcatgtat atatctgtag ctgaaagaac
    40201 agaagaaatt gcgatacgac gtgcatttgg tgcaaaaagt cgagatattg aactacaatt
    40261 tttaatagag agcattttaa tatgtgtgac aagtggtttt attggactta ttttaggtgt
    40321 tgtgtttgca acaataattg atgtattaac accagattat attaaaagtg tagtaagttt
    40381 gagttctgtc ataattgcgg ttagtgtgtc gatattaatt ggacttcttt ttggatggat
    40441 accagctcgc gcagcatcta agaaagagct catagatatt ataaaatagt aaatttaaaa
    40501 tggtaaaagt actgaagact aattatcata ttaaactgac tatcaacacc aacgatggaa
    40561 atgcgtatta gaataaaagt aagatatgta ttgcaaaatg taaattatat attgaattta
    40621 cgcaaaaaat aacatattac tt
//
LOCUS       DAOBBH010000003        40558 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.003, whole genome shotgun sequence.
ACCESSION   DAOBBH010000003 DAOBBH010000000
VERSION     DAOBBH010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 40558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40558
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.003"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(395..1312)
                     /locus_tag="P6L01_000080"
     CDS             complement(395..1312)
                     /locus_tag="P6L01_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDP3029183.1"
                     /translation="MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLS
                     ENEGDIIKYCQEIKTKNYAKDVDILFILGGDGTVNELINGVMSHDLQLPIGILPGGTF
                     NDFTKTLNIAPNHKQASEQMISAQVGTYDVIKINNQYALNFVGLGLIVQNAENVQDGS
                     KDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILTANGPFIGGSRIPLTD
                     LSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPAMKV
                     DIDGEISLETPIDIEVIPNAIQLLTVNDL"
     gene            1403..1504
                     /locus_tag="P6L01_000081"
     CDS             1403..1504
                     /locus_tag="P6L01_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029184.1"
                     /translation="MVITDIDVYRVGRENIVIAVYNEHIKLLFILIS"
     gene            complement(1582..3087)
                     /locus_tag="P6L01_000082"
     CDS             complement(1582..3087)
                     /locus_tag="P6L01_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDP3029185.1"
                     /translation="MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMR
                     ALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLG
                     AVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFV
                     IFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNLGSVGMK
                     PTNPLTPAEKKKYGLIIGSVVLAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYF
                     TLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGW
                     KTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGI
                     VGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNA
                     SAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAFSPLIIKAMKGIR"
     gene            complement(3428..3928)
                     /gene="queF"
                     /locus_tag="P6L01_000083"
     CDS             complement(3428..3928)
                     /gene="queF"
                     /locus_tag="P6L01_000083"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDP3029186.1"
                     /translation="MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDY
                     FVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVESKSLKLYLFSFRNHGDFHEDCM
                     NIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEKMAEHRLMNHDLYP
                     EKIDNR"
     gene            complement(3948..4814)
                     /locus_tag="P6L01_000084"
     CDS             complement(3948..4814)
                     /locus_tag="P6L01_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDP3029187.1"
                     /translation="MNPKVKGIIAILISAIGFSFMSVFFRLAGDLPVFQKSLARNLVA
                     MFIPLFFLYKYHQPMFGRLSSQPLLITRSTLGLIGVLLNIYAIDHMVLSDADSLMKLN
                     PFWTILLSIVFLHEKVRKYQITAMIIAILGMLLIVKPEFSSSMIPSLAGLFSGIFAAS
                     AYTCVRALSSREAPYTIVFYFSLFSVIVLIPFTAYTYEPMSQMQILYLLGAGLAAAVG
                     QIGVTLAYSFAAAKDISIFTYASIIFTAILGFVLFGESPDFYATLGYVVIIGASYYMF
                     EKARRDAKIIKK"
     gene            5615..6013
                     /gene="nrdI"
                     /locus_tag="P6L01_000085"
     CDS             5615..6013
                     /gene="nrdI"
                     /locus_tag="P6L01_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDP3029188.1"
                     /translation="MKIIYFSFTGNVRRFIKRTELENTLEITAENCMEPVHEPFIIVT
                     GTIGFGEVPEPVQSFLEVNHQYIRGVAASGNRNWGLNFAKAGRTISEEYNVPLLMKFE
                     LHGKNKDVIEFKNKVGNFNENHGREKVQSY"
     gene            5976..8081
                     /gene="nrdE"
                     /locus_tag="P6L01_000086"
     CDS             5976..8081
                     /gene="nrdE"
                     /locus_tag="P6L01_000086"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDP3029189.1"
                     /translation="MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVK
                     DKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSISFNFASYMSASKFF
                     KDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
                     GRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIK
                     GVAKGVLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRL
                     STISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVE
                     KKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQET
                     SIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAP
                     GVRKANSELHSVGLGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIA
                     KERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWKKLQQDV
                     EQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTM
                     WYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKS
                     LYYTRNKLLSVEECTSCSI"
     gene            8199..9170
                     /gene="nrdF"
                     /locus_tag="P6L01_000087"
     CDS             8199..9170
                     /gene="nrdF"
                     /locus_tag="P6L01_000087"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDP3029190.1"
                     /translation="MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSE
                     AEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHI
                     FTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFL
                     ETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEK
                     QKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEE
                     REFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK"
     gene            9351..9506
                     /locus_tag="P6L01_000088"
     CDS             9351..9506
                     /locus_tag="P6L01_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029191.1"
                     /translation="MFYFTLSKQNTHSEKLNFSLIANINKIDTKLFERLLKLWSKNAT
                     INEKYYQ"
     gene            9545..10516
                     /locus_tag="P6L01_000089"
     CDS             9545..10516
                     /locus_tag="P6L01_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3029192.1"
                     /translation="MKYLLKGNILLLLLILLTIISLFIGVSELSIKDLLHLTESQRNI
                     LFSSRIPRTMSILIAGSSLALAGLIMQQMMQNKFVSPTTAGTMEWAKLGILIALLFFP
                     TGHILLKLVFAVICSICGTFLFVKIIDFIKVKDVIFVPLLGIMMGGIVASFTTFISLR
                     TNAVQSIGNWLNGNFAIITSGRYEILYLSIPLLALTYLFANHFTIVGMGKDFTNNLGL
                     SYEKLINIALFITATITALVVVTVGTLPFLGLVIPNIISIYRGDHLKNAIPHTMMLGA
                     IFVLFSDIVGRIVVYPYEINIGLTIGVFGTIIFLILLMKGRKNYAQQ"
     gene            10503..11459
                     /locus_tag="P6L01_000090"
     CDS             10503..11459
                     /locus_tag="P6L01_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDP3029193.1"
                     /translation="MRNNNKKIMLLIAVTLLISMLYLFVGIDFEIFEYQFSSRLRKFI
                     LIILVGAAIATSVVIFQAITNNRLLTPSIMGLDAVYLFIKVLPVFLFGIQSVWVTNVY
                     LNFILTLITMVLFALILFQGIFKIGHFSIYFILLIGVLLGTFFRSITGFIQLIMDPES
                     FLAIQSSMFANFNASNSNLVTFSAVLLVILLVITILLLPYLDVLLLGRAEAINLGISY
                     EKLTRILLVIVSVLVSVSTALVGPITFLGLLTVNLAHELMKTYEHKYILIATICLSWI
                     SLFSAQWVVENVFEATTEMSILIDLIGGSYFIYLLVRRRNAQ"
     gene            11456..12217
                     /locus_tag="P6L01_000091"
     CDS             11456..12217
                     /locus_tag="P6L01_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3029194.1"
                     /translation="MIQVENLTKTINNQMILEDISIDIEKGKLTSLIGPNGAGKSTLL
                     SAICRLIRFDNGEVKIDGQLMSDYKNNDLSKKISILKQTNHTEMNITVEQLVNFGRFP
                     YSKGRLTKEDHDIVNDALDLLQLQDIRNRNIKSLSGGQRQRAYIAMTIAQDTEYILLD
                     EPLNNLDMKHAVQIMQTLKMLAHKMNKAIVIVLHDINFASCYSDQIVALKNGQLVKSD
                     LKDNVIQSSVLSDLYDMNIQIEHIRNQRICLYFKD"
     gene            12336..13364
                     /locus_tag="P6L01_000092"
     CDS             12336..13364
                     /locus_tag="P6L01_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDP3029195.1"
                     /translation="MKKTVLYLVLAVMFLLAACGNNSDKEQSKSETKGSKDTVKIENN
                     YKMRGEKKDGSDAKKVKETVEVPKNPKNAVVLDYGALDVMKEMGLSDKVKALPKGEGG
                     KSLPNFLESFKDDKYTNVGNLKEVNFDKIAATKPEVIFISGRTANQKNLDEFKKAAPK
                     AKIVYVGADEKNLIGSMKQNTENIGKIYDKEDKAKELNKDLDNKIASMKDKTKNFNKT
                     VMYLLVNEGELSTFGPKGRFGGLVYDTLGFNAVDKKVSNSNHGQNVSNEYVNKENPDV
                     ILAMDRGQAISGKSTAKQALNNPVLKNVKAIKEDKVYNLDPKLWYFAAGSTTTTIKQI
                     EELDKVVK"
     gene            complement(13681..13995)
                     /locus_tag="P6L01_000093"
     CDS             complement(13681..13995)
                     /locus_tag="P6L01_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029196.1"
                     /translation="MPKVYGSLIDTETRCRHYFTEEDIIAIKFKCCNKYYPCYKCHNE
                     FEKHAIKRWSEPSFNEKAILCGVCKHELTINEYMMVERCPNCQSRFNNRCKYHYHIYF
                     EI"
     gene            complement(14013..14936)
                     /locus_tag="P6L01_000094"
     CDS             complement(14013..14936)
                     /locus_tag="P6L01_000094"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDP3029197.1"
                     /translation="MINKDIYQALQQLIPNEKIKVDEPLKRYTYTKTGGNADFYITPT
                     KNEEVQAVVKYAYQNEIPVTYLGNGSNIIIREGGIRGIVISLLSLDHIDVSDDAIIAG
                     SGAAIIDVSRVARDYALTGLEFACGIPGSIGGAVYMNAGAYGGEVKDCIDYALCVNEQ
                     GSLIKLTTKELELDYRNSIIQKEHLVVLEAAFTLAPGKMTEIQAKMDDLTERRESKQP
                     LEYPSCGSVFQRPPGHFAGKLIQDSNLQGHRIGGVEVSTKHAGFMVNVDNGTATDYEN
                     LIHYVQKTVKEKFGIELNREVRIIGEHPKES"
     gene            complement(15063..15581)
                     /locus_tag="P6L01_000095"
     CDS             complement(15063..15581)
                     /locus_tag="P6L01_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDP3029198.1"
                     /translation="MYSNVQPFINSDLTNCFQSQYTEIESLLFNLLDSPVKYTKHIGG
                     THHFKYSTEPILDILVGVENLHDITSLDEKRLNYVGFYRLHHKYTKKVMMAKFNNFHE
                     LKQQVRLHIIQMNTQTFESYLALDYLLSTNDDIALQFNTKKHQILQHSLTIRQYEMQK
                     QQYFEKLCKKYL"
     gene            15701..16579
                     /locus_tag="P6L01_000096"
     CDS             15701..16579
                     /locus_tag="P6L01_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EMYY motif lipoprotein"
                     /protein_id="HDP3029199.1"
                     /translation="MKKIVIIAVLAILFVVISACGNKEKEAQHQFTKQFKDVEQKQKE
                     LQHVMDNIHLKEIDHLSKTDTTDKNSKEFKALQEDVKNHLIPKFEAYYKSAKNLPDDT
                     MKVKKLKKEYMTLANEKKDAIYQLKKFIGLCNQSIKYNEDILDYTKQFEKNRYKVESE
                     IKLADNKSEATNLTTKLEHNNKALRDTAKKNLDDSKENEVKGAIKNHIMPMIEKQITD
                     INQTNISDKHVNNARKNAIEMYYSLQNYYNTRIETIKVSEKLSKVDVDKLPKKGIDIT
                     HGDKAFEKKLEKLEEK"
     gene            16733..17053
                     /gene="ytxJ"
                     /locus_tag="P6L01_000097"
     CDS             16733..17053
                     /gene="ytxJ"
                     /locus_tag="P6L01_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HDP3029200.1"
                     /translation="MAIKLSSIDQFEQVIEENKYVFVLKHSETCPISANAYDQFNKFL
                     YERDMDGYYLIVQQERDLSDYIAKKTNVKHESPQAFYFVNGEMVWNRDHGDINVSSLA
                     QAEE"
     gene            17477..18601
                     /locus_tag="P6L01_000098"
     CDS             17477..18601
                     /locus_tag="P6L01_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDP3029201.1"
                     /translation="MKVLVAMDEFHGIISSYQANRYVEEAVASQIETADVVQVPLFNG
                     RHELLDSVFLWQSGQKYRIPVHDADMNEVEGVYGQTDTGMTVIEGNLFLKGKKPIVER
                     TSYGLGEMIKHALDNDAKHVVISLGGIDSFDAGAGMLQALGAQFYDDEGRVVDMRQGA
                     GVIKYIRRMDMSNLHPKMETARIQVMSDFSSRLYGKQSEIMQTYDAHQLHHNQAAEID
                     NLIWYFSELFKSELKIAIGPVERGGAGGGIAAVLNGLYQAEILTSHALVDQLTHLENL
                     VEQADLIIFGEGLNENDQLLETTTLRIAELCHKHQKVAIAICATAEKFDLFESQGVTA
                     MFNTFIDMPETYTDFKMGLQIRHYTVQSLKLLKTHFNVEV"
     gene            complement(18788..20014)
                     /gene="pepT"
                     /locus_tag="P6L01_000099"
     CDS             complement(18788..20014)
                     /gene="pepT"
                     /locus_tag="P6L01_000099"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDP3029202.1"
                     /translation="MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQ
                     QLGLPTDLDENGYLFATLESNIDADVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKP
                     YKLGNTKRVLDPKVFPELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPE
                     IKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITC
                     HGVNVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATL
                     QYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIID
                     IPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEK
                     AVQVIIGIVEDIAENH"
     gene            complement(20028..20522)
                     /locus_tag="P6L01_000100"
     CDS             complement(20028..20522)
                     /locus_tag="P6L01_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDP3029203.1"
                     /translation="MLFYLFHFTISFISTVLFSIIFNAPKRLLVACGFVGAIAWTIYQ
                     LTVDLEFGKVGASFLGSLILGLMSHTMSRRYKRPVIIFIVPGIIPLVPGGAAYQATRF
                     LVSNDYTSAVNTFLEVTLISGAIAFGILVSEILYYLYTRIKQLYGKIKGKTYKKSYNM
                     NNRV"
     gene            complement(20540..21301)
                     /locus_tag="P6L01_000101"
     CDS             complement(20540..21301)
                     /locus_tag="P6L01_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDP3029204.1"
                     /translation="MPDSITIIDENKVIDVVLIAGRILLESGAETYRVEDTMNRIAHS
                     YGLHNTYSFVSSTAIIFSLNDRTSTRLIRVQERTTDLEKIALTNSLSRKISNKELTID
                     EAKSEFIHLQHASLQYSFLTNFFAAAIACGFFLFMFGGVASDCWIAVIAGGSAFLTFS
                     FVQRYIQIKFFSEFVAAAVVISIAATFTKLGIATNQDIITIASVMPLVPGILITNAIR
                     DLLAGELLAGMSRGVEAALTAFAIGAGVAIVLLII"
     gene            complement(21497..22567)
                     /locus_tag="P6L01_000102"
     CDS             complement(21497..22567)
                     /locus_tag="P6L01_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDP3029205.1"
                     /translation="MFEAFIYNISVIVAGIYLFHRLQYSENKRMVFSKAYVTVLMTIV
                     SLLLSVYPIPYREDYLIHLTFVPLLFLGRFTNMVYTLSATVIVAIVEIVVFNNSIMYG
                     VTLIVIAAVTSAIGPFLKQNDVLSLLILNVVTIIILFGVALVSPIYTLSEVIILIPIS
                     LIITLASAITFVDIWHFFSLVNRYENEDKYDYLTGLGNVKEFDRHLNEISRKAEKEHQ
                     SIALLLIDIDGFKDVNDTYSHKSGDAVLKQMSQLLKNYVPNQFKIFRNGGEEFSVVIH
                     NYSLDQSVKLAENIRSGVEKSSFHLPNKEVIKLSVSIGVGYLTDDDPKSQRKVFKDAD
                     DMVHVAKNQGRNKVMFNPIINL"
     gene            22885..23940
                     /locus_tag="P6L01_000103"
     CDS             22885..23940
                     /locus_tag="P6L01_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDP3029206.1"
                     /translation="MVTLLLVAVTMIVSLTITPIVIAISKRLNLVDKPNFRKVHTKPI
                     SVMGGTVILFSFLIGIWIGHPIETEIKPLIIGAIIMYVLGLVDDIYDLKPYIKLAGQI
                     AAALVVAFYGVTIDFISLPMGTTIHFGFLSIPITVIWIVAITNAINLIDGLDGLASGV
                     SAIGLITIGFIAILQANIFITMICCVLLGSLIGFLFYNFHPAKIFLGDSGALMIGFII
                     GFLSLLGFKNITIIALFFPIVILAVPFIDTLFAMIRRVKKGQHIMQADKSHLHHKLLA
                     LGYTHRQTVLLIYSISILFSLSSIILYVSPPLGVVLMFVLIIFSIELIVEFTGLIDNN
                     YRPILNLISRKSSHKED"
     gene            complement(24105..24746)
                     /locus_tag="P6L01_000104"
     CDS             complement(24105..24746)
                     /locus_tag="P6L01_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDP3029207.1"
                     /translation="MTQNIITVKKEHIIENVISKSRFIAHIKPVQNEDEAKAFIAAIK
                     KEHKDATHNCSAYTIGPEMNIQKANDDGEPTGTAGVPMLDILKKLDVHNACVVVTRYF
                     GGIKLGGGGLIRAYSGAVRDVIYDVGRVELREAVPCIVTLNYDQTGKFEYELASTHFM
                     LRNQTYTDKVSYYIDVVKSDYEAFINFLNQMTAGNFDLTEETPKQLPFDIPTE"
     gene            24890..25756
                     /gene="fakB1"
                     /locus_tag="P6L01_000105"
     CDS             24890..25756
                     /gene="fakB1"
                     /locus_tag="P6L01_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB1"
                     /protein_id="HDP3029208.1"
                     /translation="MKIAVMTDSTSYLSQDLIDKYNIQIAPLSVTFDDGKNFTESNEI
                     AIEEFYNKMASSQTIPTTSQPAIGEWITKYEMLRDQGYTDIIVICLSSGISGSYQSSY
                     QAGEMVEGVNVHAFDSKLAAMIEGCYVLRAIEMVEEGYEPQQIIDDLTNMREHTGAYL
                     IVDDLKNLQKSGRITGAQAWVGTLLKMKPVLKFEDGKIIPEEKVRTKKRAIQTLEKKV
                     LDIVKDFEEVTLFVINGDHFEDGQALYKKLQEDCPSGYQVAYSEFGPVVAAHLGSGGL
                     GLGYVGRKIRLT"
     gene            26120..27202
                     /locus_tag="P6L01_000106"
     CDS             26120..27202
                     /locus_tag="P6L01_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDP3029209.1"
                     /translation="MDNVTRYKITESSQSSSQAYYHLSFELSEQQSYASEHIVRAIRM
                     RQTILLYAVTGAGKTEMMFQGIQYARRQGDNIAIVSPRVDVVVEISKRIKDAFLNEDI
                     DILHQQSSQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKSLQQALKSSSK
                     VEHATIYMTATPPKQLLSEIPHENIIKLPARFHKKSLPVPKYRYFKLNNNKIQKMLYR
                     ILQDQINNQRYTLVFFNNIETMIKTFSVYKQKITKLTYVHSEDVFRFEKVEQLRNGHF
                     DVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKLECPAGKVLFFHEGV
                     SMNMIQAKKEIQRMNKLALKRGWIDE"
     gene            27195..27869
                     /locus_tag="P6L01_000107"
     CDS             27195..27869
                     /locus_tag="P6L01_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDP3029210.1"
                     /translation="MNNCLSCGAKLYENITIYNLFKKPNRLCDRCKENWDNIKLDIKA
                     RRCSRCLKHLNQDEAYCLDCKFLSAHFNLMEQLYCQFQYDGLMKEMIHQYKFLKDYYL
                     CELLAHLIEIPQTSYDYIVPIPSSPAHDLSRTFNPVEAVLKAKGIRFDKILKMSNRPK
                     QSHLTKKERLADENPFIIDTELDLNGKEILLVDDIYTTGLTIHRAGCKLYAKNIRKFK
                     VFAFAR"
     gene            27930..28502
                     /gene="raiA"
                     /locus_tag="P6L01_000108"
     CDS             27930..28502
                     /gene="raiA"
                     /locus_tag="P6L01_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDP3029211.1"
                     /translation="MIRFEIHGDNLTITDAIRNYIEEKIGKLERYFNDVPNAVAHVKV
                     KTYSNSATKIEVTIPLKNVTLRAEERNDDLYAGIDLINNKLERQVRKYKTRINRKSRD
                     RGDQEVFVAELQEMQETQVDNDAYDDNEIEIIRSKEFSLKPMDSEEAVLQMNLLGHDF
                     FVFTDRETDGTSIVYRRKDGKYGLIQTSEQ"
     gene            28916..31447
                     /gene="secA"
                     /locus_tag="P6L01_000109"
     CDS             28916..31447
                     /gene="secA"
                     /locus_tag="P6L01_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDP3029212.1"
                     /translation="MGFLSKILDGNNKEIKQLGKLADKVIALEEKTAILTDEEIRNKT
                     KQFQTELADIDNVKKQNDYLDKILPEAYALVREGSKRVFNMTPYKVQIMGGIAIHKGD
                     IAEMRTGEGKTLTATMPTYLNALAGRGVHVITVNEYLSSVQSEEMAELYNFLGLTVGL
                     NLNSKTTEEKREAYAQDITYSTNNELGFDYLRDNMVNYSEDRVMRPLHFAIIDEVDSI
                     LIDEARTPLIISGEAEKSTSLYTQANVFAKMLKQDEDYKYDEKTKAVHLTEQGADKAE
                     RMFKVENLYDVQNVDVISHINTALRAHVTLQRDVDYMVVDGEVLIVDQFTGRTMPGRR
                     FSEGLHQAIEAKEGVQIQNESKTMASITFQNYFRMYNKLAGMTGTAKTEEEEFRNIYN
                     MTVTQIPTNKPVQRNDKSDLIYISQKGKFDAVVEDVVEKHKAGQPVLLGTVAVETSEY
                     ISNLLKKRGIRHDVLNAKNHEREAEIVAGAGQKGAVTIATNMAGRGTDIKLGEGVEEL
                     GGLAVIGTERHESRRIDDQLRGRSGRQGDKGDSRFYLSLQDELMIRFGSERLQKMMSR
                     LGLDDSTPIESKMVSRAVESAQKRVEGNNFDARKRILEYDEVLRKQREIIYNERNSII
                     DEEDSSQVVDAMLRSTLQRSINYYINTADDEPEYQPFIDYINDIFLQEGDITEDDIKG
                     KDAEDIFEVVWAKIEAAYQSQKDILEEQMNEFERMILLRSIDSHWTDHIDTMDQLRQG
                     IHLRSYAQQNPLRDYQNEGHELFDIMMQNIEEDTCKFILKSVVQVEDNIEREKTTEFG
                     EAKHVSAEDGKEKVKPKPIVKGDQVGRNDDCPCGSGKKFKNCHGK"
     gene            31489..31593
                     /locus_tag="P6L01_000110"
     CDS             31489..31593
                     /locus_tag="P6L01_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029213.1"
                     /translation="MLWEESFYFTSVKYFKKCEEVVKRCYVLSFKKSV"
     gene            31756..32866
                     /gene="prfB"
                     /locus_tag="P6L01_000111"
     CDS             join(31756..31827,31829..32866)
                     /gene="prfB"
                     /locus_tag="P6L01_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667688.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDP3029214.1"
                     /translation="MELSEIKRNIDKYNQDLTQIRGSLDLENKETNIQEYEEMMAEPN
                     FWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDLEQEV
                     INFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGF
                     KVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFAS
                     CDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQN
                     ERSQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMV
                     KDHRTNEETGKVDAVMDGDIGPFIESYLRQTMSHD"
     gene            33020..33127
                     /locus_tag="P6L01_000112"
     CDS             33020..33127
                     /locus_tag="P6L01_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029215.1"
                     /translation="MCLLKQHMQCAFSNITYGDFQGIELIVENVLNYMV"
     gene            33275..34114
                     /locus_tag="P6L01_000113"
     CDS             33275..34114
                     /locus_tag="P6L01_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDP3029216.1"
                     /translation="MKKTLTVTVSSVLAFLALNNAAHAQQHGTQVKTPVQHNYVSNVQ
                     AQTQSPTTYTVVAGDSLYKIALEHHLTLNQLYSYNPGVTPLIFPGDVISLVPQNKVKQ
                     TKAVKSPVRKASQAKKVVKQPVQQASKKVVVKQAPKQAVAKTVNVAYKPAQVQKSVPT
                     VPVAHNYNKSVANRGNLYAYGNCTYYAFDRRAQLGRSIGSLWGNANNWNYAAKVAGFK
                     VDKTPEVGAIFQTAAGPYGHVGVVESVNPNGTITVSEMNYAGFNVKSSRTILNPGKYN
                     YIH"
     gene            34288..34938
                     /locus_tag="P6L01_000114"
     CDS             34288..34938
                     /locus_tag="P6L01_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDP3029217.1"
                     /translation="MGVHQYFKRLSDMERLIRLPGKFKYFEHNVAAHSFKVTKIAQYL
                     ATVEEYHGRKINWKSLYEKALNHDFAEVFTGDIKTPVKYASSELKKLFSQVEEEMVET
                     FIEEEIPLQYRDVYKQRLQEGKDDSLEGQILSVADKIDLLYETFGEIQKRNPEELFFE
                     IYEMSLETIIQFDHLASVQDFINNIIPEMLTENFIPRTELRETTMNILNKRKEENE"
     gene            34935..35171
                     /locus_tag="P6L01_000115"
     CDS             34935..35171
                     /locus_tag="P6L01_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbA family protein"
                     /protein_id="HDP3029218.1"
                     /translation="MIWYFSAAFFPCVLVVLFSVITRSKWVGTILTLILIGASIYKEY
                     FHNEWIIFIDVVSLLAGYLIIDQLEFHKHQDEDR"
     gene            35440..37425
                     /gene="uvrB"
                     /locus_tag="P6L01_000116"
     CDS             35440..37425
                     /gene="uvrB"
                     /locus_tag="P6L01_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002473941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDP3029219.1"
                     /translation="MVEHYPFKIHSDFEPQGDQPQAIKEIVDGIKAGKRHQTLLGATG
                     TGKTFTMSNVIKEVGKPTLIIAHNKTLAGQLYSEFKEFFPENRVEYFVSYYDYYQPEA
                     YVPSTDTFIEKDASINDEIDQLRHSATSALFERDDVIIIASVSCIYGLGNPEEYKDLV
                     VSVRVGMEMDRSELLRKLVDVQYTRNDIDFQRGTFRVRGDVVEIFPASKEELCIRVEF
                     FGDEIDRIREVNYLTGEVLKEREHFAIFPASHFVTREEKLKVAIERIEKELEERLKEL
                     RDENKLLEAQRLEQRTNYDLEMMREMGFCSGIENYSVHLTLRPLGSTPYTLLDYFGDD
                     WLVMIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLV
                     YVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNERV
                     LVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLR
                     EGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAI
                     DETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKER
                     QKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG"
     gene            37433..40279
                     /gene="uvrA"
                     /locus_tag="P6L01_000117"
     CDS             37433..40279
                     /gene="uvrA"
                     /locus_tag="P6L01_000117"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDP3029220.1"
                     /translation="MKEPSIVVKGARAHNLKDIDIELPKNKLIVMTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDTIEGLSPAISIDQKTTSKNPRSTVAT
                     VTEIYDYIRLLYARVGKPYCPNHNIEIESQTVQQMVDRIMELEARTKIQLLAPVIAHR
                     KGSHEKLIEDIGKKGYVRLRIDGEIVDVNDVPTLDKNKNHTIEVVVDRLVVKDGIETR
                     LADSIETALELSEGQLTVDVIDGEDLKFSESHACPICGFSIGELEPRMFSFNSPFGAC
                     PTCDGLGQKLTVDVDLVVPDKDKTLNEGAIEPWIPTSSDFYPTLLKRVCEVYKINMDK
                     PFKKLTERQRDILLYGSGDKEIEFTFTQRQGGTRKRTMVFEGVVPNISRRFHESPSEY
                     TREMMSKYMTELPCETCHGKRLSREALSVYVGGLNIGEVVEYSISQALNYYKNIDLSE
                     QDQAIANQILKEIISRLTFLNNVGLEYLTLNRASGTLSGGEAQRIRLATQIGSRLTGV
                     LYVLDEPSIGLHQRDNDRLINTLKEMRDLGNTLIVVEHDDDTMRAADYLVDIGPGAGE
                     HGGQIVSSGTPQKVMKDKKSLTGQYLSGKKRIDVPEYRRPASDRKISIRGARSNNLKG
                     IDVDIPLSIMTVVTGVSGSGKSSLVNEVLYKSLAQKINKSKVKPGLYDKIEGIDQLDK
                     IIDIDQSPIGRTPRSNPATYTGVFDDIRDVFAQTNEAKIRGYQKGRFSFNVKGGRCEA
                     CKGDGIIKIEMHFLPDVYVPCEVCDGKRYNRETLEVTYKGKNIADILEMTVEEATQFF
                     ENIPKIKRKLQTLVDVGLGYVTLGQQATTLSGGEAQRVKLASELHKRSTGKSIYILDE
                     PTTGLHVDDISRLLKVLNRLVENGDTVVIIEHNLDVIKTADYIIDLGPEGGSGGGTIV
                     ATGTPEDIAQTKSSYTGKYLKEVLERDKQNTEDK"
BASE COUNT        14563 a         5835 c         7216 g        12944 t
ORIGIN      
        1 atcatttctg tcccactccc tcctattttt aagtacacat tagctttgac taatgataaa
       61 gaatcgctac ataatcaatc attagtcgtt ctttatcatt tccgtcccgc tctcaataaa
      121 tgttagtcta tctcattatt ataaatcgga tgaatgtgtt aatctatggc agattacacg
      181 tcatccgatt ttttatagaa tttgaaaaag acgcataaac cactatgatt taaaatacaa
      241 catcaatcat tttagtggca tgcgccaaaa ttatatgtct gtttttgaaa cagggtaata
      301 gcttaaagct aataaaaacg aatataaggt gcgttgaatc ttatgattac actccaaacc
      361 taatataata tcgggtcaag atcattccgg atgcttacaa atcattgaca gtaagtaact
      421 gaatagcatt tggtataacc tcaatatcaa taggtgtttc taatgaaatt tcgccatcaa
      481 tatcaacttt cattgctgga tctgttgtaa gtgaaatctt tttaccaggt atatgctcaa
      541 taccttgagt aatttcattc caattcatgc tatcacgctt tttaaaaata tcatttaaaa
      601 tactgaaact ttgttcatta aaaatgaaag tgttcagttc accatcttga ggagacaaat
      661 cagtcaatgg tatacgacta ccaccaatga atggaccatt tgctgttagt atcatggtcg
      721 tttcgccaga atatgtctta tcatctattg ataattgata attaaattgt gttggattta
      781 gcagtgtttt gacagttgat ccaatataac tcaatttacc aaatatatct tttgaaccat
      841 cttgtacgtt ttcagcgttt tgaacaatga gacctaagcc aacaaagttg agtgcatatt
      901 gattatttat tttaattaca tcgtatgtac caacttgtgc agaaatcatt tgttcactag
      961 cttgtttatg attaggtgct atatttagcg tttttgtaaa atcattaaaa gtaccgcctg
     1021 gtaaaatgcc aatagggagt tgaaggtcat gtgacataac accatttata agttcgttaa
     1081 ccgtgccatc accgccaaga ataaataata tatctacatc ttttgcatag tttttagttt
     1141 tgatttcttg gcaatattta ataatgtcac cttcgttttc actcaattga atagaaagat
     1201 gcttacaaat tgaacttaat gctgttgtaa cttccccaat accttgatta atatttttta
     1261 atccactgtg ttcatggtaa aagaggacac catgtgtata tttattttcc atagtttagc
     1321 ctactttcta aaaattggtt cattaaatat atatacccac ttttaattgt taataccaaa
     1381 aatatgtttt taaatagaga aaatggtaat aactgacatt gatgtctata gagtgggacg
     1441 agaaaatata gttatagctg tctataacga gcatattaag cttttattta tactgatatc
     1501 ttgaatttaa ttaatagaaa cctataaaaa aacagtaagc catttaaatg acttactgtt
     1561 ttttgaatta ggccaacaat attaacgtat acctttcatc gctttgatga ttaaaggtga
     1621 gaatgctaat acaattgttg taacaataat tgcaacaaca cctaggaaaa taaagtaatt
     1681 tgtttgacct agtggttcta ttaacttaac taaagtacca ttgattgctt gtgcagaagc
     1741 gttagttaag taccaaatac tcatcatttg ggcattaaat gctttaggtg ctaacttaac
     1801 agcagcacta ttacccgttg gtgataagca tagctcaccg ataacacaaa taatgtacga
     1861 taaaataacc cagttaactg agaagtttga tgaacctgat gcataaccta caataccaat
     1921 tagtatgtat gacgcacctg ctaagaacgt accaattgca aattttactg gcaggctagg
     1981 ttgtttagtt ccaagctttt gccataaaag tgaaataatt ggagctaata ataaaataaa
     2041 taatgggtta attgattgga agatcgcttc accaaagttt gttttccaac caaataagtt
     2101 taatttcata tctgaatgtt caattccata tatgtttaat acattagacc cttgttcttg
     2161 aatagcccag aacaccattc caagaataaa taatggaata aatgctttaa cacgagaacg
     2221 ttcagtatca gtgacatctt tacttctaat aattaaagtg aagtaaatga ttggtaatgc
     2281 aatacctaat actaaaacag tattactaac taagttaaat gataatgagt tagttaatgc
     2341 accaataacg ataattaata caattgctaa gacaacactt ccgataataa gaccatactt
     2401 tttcttttca gctggtgtca atgggttagt aggtttcata ccaacgctac ctaagttttt
     2461 gcggttgaaa agtacatacc atactaaacc taatgccata ccaactgctg caatcaagaa
     2521 tccgccgtgg aagtttttaa cattaacaaa gtgttgcaaa ataataggtg ataataatgc
     2581 acccatatta actgacatat agaaaataac aaaacctgca tccatacgtc tatcattttc
     2641 aggatataaa cggccaacga tatttgaaat gtttggcttc attaaacctg aaccaataat
     2701 gatgaagaac attgatgtga ataagccgat taatgcaaat ggtaagctta aacaaatatg
     2761 tccgataata ataaagactg cacctaataa agtagcgcct ctagtgcctg taattctgtc
     2821 agcaatccat ccgcctggta ttgatgtcat atagattaat gaaccataaa ctgacataat
     2881 tgacatagct gttgttttat caattccaag gccattatct gttacggcaa agtacatgta
     2941 gaaaatgagt agggcacgca tgccataata actaaacctt tcccagaact ctacaaagaa
     3001 gagtacgcct agtcctcgag gatgcccgaa aaatcctgtt tgaggtatgt cttgaatttg
     3061 atttccatgg gagttttgtt gtgtcatgta tacatcccat cctttcttcc cctaatacaa
     3121 tagtttaagt aaatttgatt gtgccttatc aactaaatat tccaacaaat cacaaaaaaa
     3181 taaaaatatt gagaatattc tgttattaag cgcgcacaat taattagaga taatataaaa
     3241 atatacttat tgacgttaaa gtgcaatagg tttctataaa aatgttgttt aattcgtgta
     3301 ctaatattct tttggtgtac tttggtataa aatgttattt taatgtaacc tattatgtat
     3361 gctaaatgaa aaacacacca ataagttagg aaaatcaact tattggtgtg tttattaaat
     3421 gatacattta acgattatct attttttcgg gatataaatc atgattcatc aaacgatgct
     3481 cagccatttt ttcatattta gaatttggac gtccatagtt tgtataagga tcaatagaaa
     3541 ttccaccacg tggtgtgaac ttgccccaga cttcaatata atgtgggtcc ataagctcta
     3601 tcaaatcatt cataataata ttcatacaat cttcgtgaaa atcaccgtga tttctgaaac
     3661 taaataagta taatttcaaa gattttgatt caaccatttt aacatttgga atatatgaaa
     3721 tatagatagt tgcaaaatct ggttgcccag taattggaca taatgatgta aattctggac
     3781 agttgaattt tacgaaatag tcacgacctt gatgcttatt atcaaacgat tctaatacat
     3841 caggacgata gtcaaaattg taagtattgt cttgatttcc taataaagtt atatcttgta
     3901 attcatcttg ttgacggcca tgtgccatat aaagcgctcc tttaaattta tttttttatt
     3961 attttggcgt ctcggcgtgc tttttcaaac atgtaataac ttgcaccgat aataacgacg
     4021 taacctaatg ttgcatagaa atctggagat tctccgaata gaacaaatcc aagtattgct
     4081 gtgaaaatta tagatgcata cgtaaaaata gaaatatctt ttgctgctgc aaaactatat
     4141 gctaaagtaa caccaatttg acccacagcg gcagctaagc cagcccctaa tagataaagt
     4201 atttgcatct gactcattgg ttcataagta tatgcagtga aaggtattaa aacgatgaca
     4261 gaaaataagg agaagtaaaa tactatagta tatggtgctt ctcttgaact aagtgctcga
     4321 acacatgtat atgctgatgc tgcaaaaata cctgagaata agccagctaa tgatggaatc
     4381 atagatgatg aaaattcagg tttcactatt aaaagcatac ctaaaatagc aattatcatt
     4441 gctgtaattt gatacttcct taccttttca tgtaagaaaa caatgcttaa taaaatcgtc
     4501 cagaaaggat tgagtttcat taatgaatcg gcatcactaa gtaccatatg atcaatggca
     4561 taaatattta acaatacacc aataagtcca agtgttgatc gtgttattaa taagggttga
     4621 cttgaaagtc tgccaaacat tggctgatgg tatttatata aaaaaaataa tggaataaac
     4681 attgctacta agtttcgtgc taatgatttt tgaaaaacag gaaggtcacc tgcaagtctg
     4741 aaaaacactg acataaaact gaaaccaata gccgaaatta aaatggcaat gatacctttt
     4801 actttaggat tcaattttat cgcctctttt atataaaatt aacgtattta tattagcata
     4861 aaacaacatg ttgtgcataa atagttgaaa tttactataa aaagactata atagactgta
     4921 ccgaacaaac gttctgtgtt tatttgtcgg aataataggg cattacactt ttatgaatgt
     4981 ttgtgttatt acataaaaca aatatcaatt cagtatcaag ctaataagct ttttcttgat
     5041 ttctgttgat acaattgaga ttgacacaga tttaaaaaaa tcaagtgata tctactaaaa
     5101 aattttttta aatttgttca agtttttcta atttagtatt ggtgcctagt tggaacgttt
     5161 tacgaacatt cgattagaaa atggcacttt aaatcatagt gtgtcttatg tataatgaaa
     5221 cacataatat agtgttggtg aaacgaaaaa gacacaatat cttgtgtttt gtatgcaaat
     5281 gctttattta tgaagaaatt acatttaaaa gtaatttaac acaggaattt aatagttatt
     5341 atcaattaat agtcatattt ttagaaaatg tactgagcaa atggaagata tccaatgatg
     5401 taaatactac atatagtgat ttttatacat tcaacccata taagctacta ttttctcaaa
     5461 tataaatcta tgcaattggt ttacatttga gaaaataagt agcttcatta tagttaatac
     5521 aatgctgaga taaccatagt aaccatgttg ttaaagcatt ttttaattgg aatgactact
     5581 ttatttaaaa gggttgaaga aagaaggtga tccaatgaaa ataatatatt tttcatttac
     5641 tggaaatgtc cgtcgtttta ttaagagaac agaacttgaa aatacgcttg agattacagc
     5701 agaaaattgt atggaaccag ttcatgaacc gtttattatc gttactggca ctattggatt
     5761 tggagaagta ccagaacccg ttcaatcttt tttagaagtt aatcatcaat acatcagagg
     5821 tgtggcagct agcggtaatc gaaattgggg actaaatttc gcaaaagcgg gtcgcacgat
     5881 atcagaagag tataatgtcc ctttattaat gaagtttgag ttacatggaa aaaacaaaga
     5941 cgttattgaa tttaagaaca aggtgggtaa ttttaatgaa aaccatggaa gagaaaaagt
     6001 acaatcatat tgaattaaat aatgaggtca ctaaacgaag agaagatgga ttctttagtt
     6061 tagaaaaaga ccaagaagct ttagtagctt atttagaaga agtaaaagac aaaacaatct
     6121 tcttcgatac tgaaatcgag cgtttacgtt atttagtaga caacgatttt tatttcaatg
     6181 tgtttgatat ttatagtgaa gcggatctaa ttgaaatcac tgattatgca aaatcaatct
     6241 cgtttaattt tgcaagttat atgtcagcta gtaaattttt caaagattac gctttgaaaa
     6301 caaatgataa aagtcaatac ttagaagact ataatcaaca cgttgccatt gttgctttat
     6361 acctagcaaa tggtaataaa gcacaagcta aacaatttat ttctgctatg gttgaacaaa
     6421 gatatcaacc agcgacacca acatttttaa acgcaggccg tgcgcgtcgt ggtgagctag
     6481 tgtcatgttt cttattagaa gtggatgaca gcttaaattc aattaacttt attgattcaa
     6541 ctgcaaaaca attaagtaaa attgggggcg gcgttgcaat taacttatct aaattgcgtg
     6601 cacgtggtga agcaattaaa ggaattaaag gcgtagcgaa aggcgtttta cctattgcta
     6661 agtcacttga aggtggcttt agctatgcag atcaacttgg tcaacgccct ggtgctggtg
     6721 ctgtgtactt aaatatcttc cattatgatg tagaagaatt tttagatact aaaaaagtaa
     6781 atgcggatga agatttacgt ttatctacaa tatcaactgg tttaattgtt ccatctaaat
     6841 tcttcgattt agctaaagaa ggtaaggact tttatatgtt tgcacctcat acagttaaag
     6901 aagaatatgg tgtgacatta gacgatatcg atttagaaaa atattatgat gacatggttg
     6961 caaacccaaa tgttgagaaa aagaaaaaga atgcgcgtga aatgttgaat ttaattgcgc
     7021 aaacacaatt acaatcaggt tatccatatt taatgtttaa agataatgct aacagagtgc
     7081 atccgaattc aaacattgga caaattaaaa tgagtaactt atgtacggaa attttccaac
     7141 tacaagaaac ttcaattatt aatgactatg gtattgaaga cgaaattaaa cgtgatattt
     7201 cttgtaactt gggctcatta aatattgtta atgtaatgga aagcggaaaa ttcagagatt
     7261 cagttcactc tggtatggac gcattaactg ttgtgagtga tgtagcaaat attcaaaatg
     7321 caccaggagt tagaaaagct aacagtgaat tacattcagt tggtcttggt gtgatgaatt
     7381 tacacggtta cctagcaaaa aataaaattg gttatgagtc agaagaagca aaagattttg
     7441 caaatatctt ctttatgatg atgaatttct actcaatcga acgttcaatg gaaatcgcta
     7501 aagagcgtgg tatcaaatat caagactttg aaaagtctga ttatgctaat ggcaaatatt
     7561 tcgagttcta tacaactcaa gaatttgaac ctcaattcga aaaagtacgt gaattattcg
     7621 atggtatggc tattcctact tctgaggatt ggaagaaact acaacaagat gttgaacaat
     7681 atggtttata tcatgcatat agattagcaa ttgctccaac acaaagtatt tcttatgttc
     7741 aaaatgcaac aagttctgta atgccaatcg ttgaccaaat tgaacgtcgt acttatggta
     7801 atgcggaaac attttaccct atgccattct tatcaccaca aacaatgtgg tactacaaat
     7861 cagcattcaa tactgatcag atgaaattaa tcgatttaat tgcgacaatt caaacgcata
     7921 ttgaccaagg tatctcaacg atcctttatg ttaattctga aatttctaca cgtgagttag
     7981 caagattata tgtatatgcg cactataaag gattaaaatc actttactat actagaaata
     8041 aattattaag tgtagaagaa tgtacaagtt gttctatcta acaattaaat gttgaaaatg
     8101 acaaacagct aatcatctgg tctgaattag cagatgatta gactgctatg tctgtatttg
     8161 tcaattattg agtaacatta caggaggaaa ttatattcat gatagctgtt aattggaaca
     8221 cacaagaaga tatgacgaat atgttttgga gacaaaatat atctcaaatg tgggttgaaa
     8281 cagaatttaa agtatcaaaa gacattgcaa gttggaagac tttatctgaa gctgaacaag
     8341 acacatttaa aaaagcatta gctggtttaa caggcttaga tacacatcaa gcagatgatg
     8401 gcatgccttt agttatgcta catacgactg acttaaggaa aaaagcagtt tattcattta
     8461 tggcgatgat ggagcaaata cacgcgaaaa gctattcaca tattttcaca acactattac
     8521 catctagtga aacaaactac ctattagatg aatgggtttt agaggaaccc catttaaaat
     8581 ataaatctga taaaattgtt gctaattatc acaaactttg gggtaaagaa gcttcgatat
     8641 acgaccaata tatggccaga gttacgagtg tatttttaga aacattctta ttcttctcag
     8701 gtttctatta tccactatat cttgctggtc aagggaaaat gacgacatca ggtgaaatca
     8761 ttcgtaaaat tcttttagat gaatctattc atggtgtatt taccggttta gatgcacagc
     8821 atttacgaaa tgaactatct gaaagtgaga aacaaaaagc agatcaagaa atgtataaat
     8881 tgctaaatga cttgtattta aatgaagagt catacacaaa aatgttatac gatgatcttg
     8941 gaatcactga agatgtgcta aactatgtta aatataatgg aaacaaagca ctttcaaact
     9001 taggctttga accttatttt gaggaacgtg aatttaaccc aatcattgag aatgccttag
     9061 atacaacaac taaaaaccat gacttcttct cagtaaaagg tgatggttat gtattagcat
     9121 taaacgtaga agcattacaa gatgatgact ttgtatttga caacaaataa caattaaatt
     9181 aaaagacctt cacatgtaaa gggaaatagc gattcgtttc gtcttgtctc ctacatgttg
     9241 aaggtctttt ctctatgctt aaaagcgtat gcatattttg aaaaagtaaa aattgtattt
     9301 aatgatgtca atgaggctag aaagtataca agatattgca ttacaaaagg gtgttctatt
     9361 ttactttaag caaacaaaat acacattccg aaaaattaaa tttcagttta attgcaaata
     9421 tcaataaaat tgacactaaa ttatttgaaa ggctattgaa attatggtca aaaaacgcta
     9481 ctattaatga gaaatattat caatgataat gattatcatt aatttaaagg gagaaaaatt
     9541 tgtaatgaag tatttattaa agggaaatat tttgcttcta ttactaatat tgttgacaat
     9601 tatttcgttg ttcataggtg tgagtgaact atcaattaaa gatttactac atttaactga
     9661 atcacagcgg aatattttat tctcaagccg aataccaagg acgatgagta ttttaattgc
     9721 tggaagttcg ttggctttag caggcttgat aatgcaacaa atgatgcaaa ataagtttgt
     9781 tagtccgact acagctggaa cgatggaatg ggctaaacta ggtattttaa ttgctttatt
     9841 gttctttcca accggtcata ttttattaaa actagtattt gctgttattt gcagtatttg
     9901 cggtacgttt ttatttgtta aaatcattga ttttataaaa gtgaaagatg tcatttttgt
     9961 accgctttta ggaattatga tgggtgggat tgttgcaagt ttcacaacct tcatctcatt
    10021 gcgcacgaat gctgttcaaa gcattggtaa ctggcttaac gggaactttg ccattatcac
    10081 aagtggacgc tatgaaattt tatatttaag tattcctctt ttagcattga catatctttt
    10141 tgctaatcat ttcacgattg taggaatggg taaagacttt actaataatt taggtttgag
    10201 ttacgaaaaa ttaattaaca tcgcattgtt tattactgca actattacag cattggtagt
    10261 ggtgactgtt ggaacattac cgttcttagg actagtaata ccaaatatta tttcaattta
    10321 tcgaggtgat catttgaaaa atgctatccc tcatacgatg atgttaggtg ccatctttgt
    10381 attattttct gatatagttg gcagaattgt tgtttatcca tatgaaataa atattggttt
    10441 aacaataggt gtatttggaa caatcatttt ccttatcttg cttatgaaag gtaggaaaaa
    10501 ttatgcgcaa caataataaa aaaataatgc ttttaattgc agtaacgtta ttaattagta
    10561 tgctgtactt atttgtaggt attgattttg aaatatttga atatcaattt tcaagtcgtt
    10621 taagaaagtt catattaatt attttagtag gtgctgccat tgcaacttca gtggtgattt
    10681 ttcaagcgat tacaaataac cgtctattga caccatcaat aatggggtta gatgcagttt
    10741 atttatttat caaagtattg ccagtctttt tatttggaat tcaatcggta tgggttacta
    10801 atgtatattt gaactttata ttaacactta taacgatggt gttattcgca ctaatcctat
    10861 tccaaggtat ctttaaaatt ggacattttt caatttattt tatcttactt attggtgtcc
    10921 ttttaggaac attttttaga agcataacag gttttattca actgattatg gatcctgagt
    10981 catttttagc aatacaaagt agtatgtttg ctaattttaa tgcttctaat tcgaatttag
    11041 ttactttctc agcagtgcta ttagtaatct tattagtcat tacaatttta ctattgcctt
    11101 atttagatgt attgctttta ggtcgtgctg aagcaattaa tcttgggata tcgtatgaaa
    11161 aattaacgcg aattctactt gtaatagtct cagttttagt ttctgtgtca actgcattag
    11221 taggaccaat tacattttta ggtttattaa ctgtaaatct agcgcatgaa ctaatgaaga
    11281 cgtatgaaca taagtatatt ttaattgcga caatttgctt gagttggatt agtttattta
    11341 gtgcgcaatg ggtagttgaa aatgtgtttg aagctacgac agaaatgagt atacttattg
    11401 atttgattgg tggaagttat ttcatttatc tattagttag aaggagaaat gcgcaatgat
    11461 tcaagttgaa aatttaacta aaactataaa taatcaaatg atattggaag atattagcat
    11521 agatatcgaa aaaggtaaat tgacttcttt aattggacct aatggtgcgg gtaagagtac
    11581 tttactttca gcgatatgta ggttaattcg ttttgataac ggtgaagtga aaatagatgg
    11641 acagctcatg tctgattata aaaataatga cttgtcgaaa aaaatatcta tattaaaaca
    11701 aacaaaccat actgaaatga atattacggt agagcagttg gtaaactttg gacgattccc
    11761 ttattctaaa ggtcgtttga cgaaagagga tcatgatatt gtcaatgatg cgctagattt
    11821 gttgcaacta caagatatca gaaatcgtaa tattaagtca ttatctggtg gacaacgtca
    11881 gcgtgcatac attgcaatga caatagcaca agatactgaa tatattttgc tagatgaacc
    11941 attaaataat ttagatatga agcatgctgt tcaaattatg caaacgttaa aaatgttagc
    12001 gcataaaatg aataaagcga ttgtcattgt gttacatgat attaactttg cgtcctgtta
    12061 ttcagatcag attgtagcat tgaaaaacgg acaactagtt aagtcagatt tgaaagataa
    12121 tgtcattcaa agtagtgttt taagtgattt atatgacatg aatattcaaa ttgaacatat
    12181 aagaaatcaa aggatttgtt tatattttaa ggattgataa tttggagaca ctttaaaggg
    12241 gtgatgcgcc aattaaagaa gggttaaacg taaagcattt atttatattt cacatcaagc
    12301 acacagatta agccaaaaga ggagaatatt atattatgaa gaaaacagtc ttatatttag
    12361 tattagcagt aatgttttta ttagcggcat gcggtaacaa ttctgataaa gaacaatcaa
    12421 aatcagaaac taaaggttct aaagatacag taaaaattga aaataactat aaaatgcgtg
    12481 gcgagaaaaa agatggtagt gacgctaaaa aagttaaaga aactgttgaa gtaccaaaaa
    12541 atcctaaaaa tgcagttgtg ttagactatg gcgcattaga tgtaatgaaa gaaatgggct
    12601 tatcagataa agtaaaagca ttacctaaag gggaaggcgg taagtcatta ccgaatttct
    12661 tagaatcatt taaagatgat aaatatacaa acgttggtaa tttaaaagaa gtgaattttg
    12721 ataaaattgc tgcgacgaaa cccgaagtaa tctttatctc tggacgtaca gctaatcaaa
    12781 agaatttaga tgaattcaaa aaagctgcac ctaaagcgaa aattgtttat gttggtgcag
    12841 atgaaaagaa cttaattggt tcaatgaaac aaaacactga aaatatcggt aaaatttacg
    12901 ataaagaaga taaagctaaa gaattaaata aagatttaga taacaaaatt gcttcaatga
    12961 aagataaaac gaaaaacttc aataaaactg ttatgtattt actagttaac gaaggtgaat
    13021 tatcaacatt tggacctaaa ggtcgttttg gtggattagt ttacgataca ttaggattca
    13081 atgcagttga taaaaaagta agtaatagca atcatggaca aaatgtttct aacgaatatg
    13141 ttaataaaga aaatccagat gttattttag cgatggatag aggtcaagcg ataagtggta
    13201 aatcaactgc gaaacaagca ttaaataatc ctgtattaaa aaatgttaaa gcaattaaag
    13261 aagacaaagt atataattta gatcctaaat tatggtactt tgcagctgga tcaactacaa
    13321 ctacaattaa acaaattgag gaacttgata aagttgtaaa ataattttaa aagaggggaa
    13381 caatggttaa aggtcttaat cattgctccc ctcttttctt taaaaaagga aatctgggac
    13441 gtcaatcaat gtcctagact ctaaaatgtt ctgttgtcag tcgttggttg aatgaacatg
    13501 tacttgtaac aagttcattt caataccagt gggctccaaa catagagaaa tttgattttc
    13561 aatttctact gacaatgcaa gttggcgggg cccaaacata gagaatttca aaaaggaatt
    13621 ctacagaagt ggtgctttat catgtctgac ccactcccta taatgttttg actatgttgt
    13681 ttaaatttca aaataaatat gatagtgata tttacagcga ttgttaaacc gagattggca
    13741 atttggacaa cgctctacca tcatatattc attgattgtt aattcgtgtt tgcatacacc
    13801 gcataagatt gctttttcgt taaatgaagg ctcagaccaa cgcttaatgg cgtgcttttc
    13861 aaactcatta tggcacttat agcatggata gtatttatta caacatttaa atttaatagc
    13921 aataatatct tcttcggtaa aataatggcg acagcgtgtt tcagtatcga ttaatgaacc
    13981 ataaacttta ggcatagaca aagctcctta acttacgatt cctttggatg ttcaccaata
    14041 atgcgaactt cacgatttaa ttcaatgcca aatttttctt tgacggtctt ttgtacataa
    14101 tgaataaggt tttcataatc tgtagcagtt ccattgtcta catttaccat aaaaccagcg
    14161 tgtttggttg aaacttcaac gccgccaata cggtgacctt gcaaattaga atcttgtatc
    14221 aatttacctg caaaatgacc aggcggtctt tggaatacac taccacatga aggatactct
    14281 aaaggttgtt tagattctct acgttctgtt aaatcatcca ttttagcttg tatttcagtc
    14341 attttaccag gagctaaagt aaatgcagct tctaatacaa ctaagtgttc tttttgaata
    14401 atgctattac gataatctaa ctctaattct tttgttgtaa gtttaattaa cgagccttgt
    14461 tcgtttacgc aaagcgcata gtctatacaa tctttaactt cgccaccata agcgccagca
    14521 ttcatataca ctgcaccacc aattgaacct ggaataccac atgcaaattc aaggccagta
    14581 agtgcgtaat cacgagcaac acgtgagaca tcaataattg cagcgccgct accggctatt
    14641 atcgcatcat cagatacatc gatatgatct agtgataata aactaattac aatgccgcga
    14701 ataccacctt cacggataat aatatttgag ccatttccta aatatgtaac aggaatctca
    14761 ttttgatagg catatttaac aactgcttgt acttcttcat ttttagtagg ggtaatataa
    14821 aagtcggcat taccacctgt tttagtataa gtgtatcgtt ttaaaggttc atcaacttta
    14881 attttttcat ttgggataag ttgttgtaaa gcttgataga tgtctttatt tatcacttct
    14941 cagtacatcc tttctcatgt ctttaatatc atatagtatt ataccaattt taaaattcat
    15001 ttgcgaaaat tgaaaagaaa gtattagaat tagtataatt ataaaatacg gcattattgt
    15061 cgttataagt attttttaca tagtttttca aagtattgtt gcttttgcat ctcatattgt
    15121 ctaattgtta agctatgttg caatatttgg tgcttttttg tattgaattg caaagcaata
    15181 tcatcattag ttgataagag gtaatcaagt gcaagataag attcaaatgt ttgggtattc
    15241 atttgaatga tatgtagacg cacctgttgt tttagttcat gaaaattgtt aaacttcgcc
    15301 atcataactt tcttagtata tttatgatgc aaacgataaa accctacata atttaagcgt
    15361 ttttcatcta aggatgtaat atcatgcaaa ttttctacac ctactaaaat atctaaaatt
    15421 ggctctgttg aatatttaaa atgatgcgta ccgccaatat gttttgtata ttttactggg
    15481 ctgtctaaga ggttgaataa taatgattca atttcagtgt attgtgattg aaaacaatta
    15541 gttaaatcac tattaatgaa tggttgaaca tttgaataca tgataaactc ctttgatatt
    15601 gaaaattaat ttaatcacga taaagtctgg aatactataa cataattcat tttcataata
    15661 aacatgtttt tgtataatga atctgttaag gagtgcaatc atgaaaaaaa ttgttattat
    15721 cgctgtttta gcgattttat ttgtagtaat aagtgcttgt ggtaataaag aaaaagaggc
    15781 acaacatcaa tttactaagc aatttaaaga tgttgagcaa aaacaaaaag aattacaaca
    15841 tgtcatggat aatatacatt tgaaagaaat tgatcatcta agtaaaactg atacaactga
    15901 taaaaatagt aaagaattta aggcactaca agaagatgtt aaaaaccatc tcatacctaa
    15961 atttgaagca tattataagt cagcaaaaaa tttgcctgat gatacaatga aagttaagaa
    16021 attaaaaaaa gaatatatga cgcttgcaaa tgagaagaag gatgcgatat atcaattaaa
    16081 aaaattcata ggtttatgta atcaatctat caagtataac gaagacattt tagattatac
    16141 gaaacaattt gaaaaaaata gatacaaagt tgaatcagaa attaaattag ctgataataa
    16201 aagtgaagca actaatctta cgacaaaatt agaacataat aataaagcgt taagagatac
    16261 tgcgaagaag aacctagatg atagtaaaga aaatgaagta aaaggcgcga ttaaaaatca
    16321 cattatgcca atgattgaaa aacaaattac cgatattaac caaactaata ttagtgataa
    16381 gcatgttaat aatgcaagga aaaacgcaat agaaatgtat tacagtctgc agaactatta
    16441 taatacacgt attgaaacaa taaaggttag tgagaagtta tcaaaagtcg atgtagataa
    16501 gttgccgaaa aagggtatag atataactca cggcgataaa gcctttgaaa aaaagcttga
    16561 aaaattagaa gaaaaataac tataatcatt tttcaaagtt aaaaattttg aatttatggt
    16621 taacatgtca acttactatg tgtataatgg taaacattga tattaactat atgtataaaa
    16681 atgtcacgca gatgctattt aaatgtgata aatattttta gaggtgaata gagtggctat
    16741 aaagctaagt tcaattgacc aatttgaaca ggttattgag gaaaataaat atgtttttgt
    16801 attaaaacat agtgaaactt gtccaatatc ggcaaatgcg tacgatcaat ttaataaatt
    16861 tttatatgaa cgcgatatgg acggttatta tttgattgtc caacaagaac gcgatttgtc
    16921 agattatatt gctaaaaaaa cgaacgttaa acatgaatca cctcaagcat tttattttgt
    16981 aaatggtgaa atggtttgga atcgagacca cggtgatatc aatgtgtcgt cattagcaca
    17041 agcagaagaa taatgaaact ataggttgga acattttgcc ttacactact agacgtgaat
    17101 agcacaactt aaattcgtgt gaatcagagt agtttggcta taatgatgtt ctgacctttt
    17161 attttatgtc acctttagaa gcagttaagt tagtactttt ttacaaacat atgtataatg
    17221 tattcgagta tttttattga aaatattttg gaaaacgacg aatccaataa gaaaatttaa
    17281 acatgatttg taagttagtt taataggaaa tatatgataa accaaaagaa gcatactgtt
    17341 atttactgga ataattaata atcatgtcat gttaaatgtt cgcatataat cacgagataa
    17401 aatctaaaat ttaagattaa tcttttatga ataaaaaacg tatcacaaca aataataaag
    17461 taaggtggtc aaggttatga aagtattagt agccatggat gagtttcatg gaattatttc
    17521 gagttatcaa gctaatagat atgttgaaga ggcagttgca agccaaattg aaactgcaga
    17581 tgtagttcaa gtaccattgt ttaatggaag acatgaatta ttagattctg tatttttatg
    17641 gcaatctggg caaaagtatc gtataccagt acatgatgca gatatgaatg aagttgaagg
    17701 tgtttacgga caaactgata cagggatgac cgttatcgag gggaatttat ttttaaaagg
    17761 taaaaaacca attgttgaac gaacaagtta tggtttagga gaaatgatta aacatgcatt
    17821 agataatgac gcaaaacatg ttgtaatttc actaggtggg attgatagtt ttgatgctgg
    17881 tgcaggtatg ttacaagcat taggtgctca attctatgat gacgaagggc gtgtcgtaga
    17941 tatgagacaa ggtgctggtg taattaaata tattcgtcgt atggatatgt cgaacttaca
    18001 ccctaaaatg gaaacagcaa gaattcaagt aatgtcggat ttttcaagtc gattatatgg
    18061 taagcaaagt gaaatcatgc aaacttatga tgcgcatcag ttgcatcata atcaagcagc
    18121 agaaatcgat aatttaattt ggtattttag tgagttattt aaaagtgaat tgaaaattgc
    18181 aattggtcca gttgaacgtg gtggtgctgg tggtggaatt gcagcagtct tgaatggact
    18241 gtatcaagct gaaatattaa ccagtcatgc attagtagac caactaacac atttagaaaa
    18301 tttagttgaa caagcggatt taattatttt tggagaagga ttaaatgaaa atgatcagtt
    18361 gctagaaacg acaacattgc gtattgcaga actttgtcat aaacatcaaa aggttgccat
    18421 tgcaatttgt gcaactgctg aaaagtttga tttatttgaa tcacaagggg ttacagcaat
    18481 gtttaataca tttatcgata tgccagaaac ttatactgac tttaaaatgg gattacaaat
    18541 taggcattat acggttcagt ctttaaaact gttgaaaaca cattttaatg ttgaggttta
    18601 gtaaagaagg actaaattga tgatgctgtc atgatggtta ataacattta tgatggttag
    18661 caaaacgaat tagaagatcg aaagtatacg taaaaaatat gaaaaatcac gctatcattg
    18721 cactgaatgt tagcgtgatt tttttattaa ttaagcctga gttgaactag tatataatcg
    18781 tttgttttta gtgattttca gcgatatctt ctacaattcc aatgattact tgtactgctt
    18841 tttccataac atcaatggat gcatattcat atgggccgtg gaagttaccg caacctgtaa
    18901 agatgtttgg agttggtaac cccataaatg acaattgtga accatctgta ccaccacgaa
    18961 taggttcagt gtttgctgga atatctaatt tggcaaagac acgtttaggt atatcaataa
    19021 tatgaggcaa tggtaatatt ttttctgcca tattgaaata ttgatccgat atatcaactt
    19081 taactggata attttcaaaa tgggcattga tatcgtcacg tatttctaaa atacgtttct
    19141 tacgcaattc gaattgtttt ttatcatgat cacgaataat gtattgcaaa gttgcttttt
    19201 caacagttcc ttcaaagttc attaagtgat aaaagccttc gtatccttct gttcgctccg
    19261 gaacttcact atcaggtagc aaactatcga attgttcacc taaacgtatt gcgtttacca
    19321 ttgcattttt agctgaacca ggatgaacat ttacaccgtg gcatgtaata accgcttcag
    19381 cagcgttaaa gctttcatat tgtaattctc catattgact accatccata gtataagcaa
    19441 aatcagcatt gaagcggtca acatcaaatt tatgtggacc acgaccgatt tcttcgtctg
    19501 gtgtaaatcc aatgcgaatg gtaccatgtt taatttctgg atgttcttgt aaataacaaa
    19561 tagcttccat aatttccaca atacccgctt tatcgtctgc acctagtaac gatgtaccat
    19621 cagttaccat taatgtatga ccaactaaac tgttaagttc tggaaatact ttaggatcta
    19681 agacacgttt agtattgcct agtttgtatg gcttaccatc atagttttca ataatttgcg
    19741 gtttaacatt tgaagcattg aaatcaggtg atgtatcaac atgcgccaaa aatccaactg
    19801 ttgggacgtc ggcatcgatg ttactttcta atgtagcaaa taagtagcca ttttcatcta
    19861 aatcagttgg caatcctaat tgttgtaatt ctttttctaa taaatgtaac aaatcccatt
    19921 gcttttcagt tgaaggtgtt gttgtagatt ttggatcaga ttgcgtatca attgtcgtat
    19981 atcttgttaa tctatctatc aattggttct tcattatatt cgacccctta aactctatta
    20041 ttcatgttgt aagatttttt atatgtctta cctttgattt taccatacag ttgtttgata
    20101 cgtgtgtata ggtaatatag aatttcagaa actaatatac cgaaagcaat cgcacctgaa
    20161 atcagtgtaa cttctaaaaa tgtatttaca gcacttgtat aatcattcga tactaaaaaa
    20221 cgagtcgctt gataagctgc accaccaggt actaatggta taatgcctgg tactatgaat
    20281 ataattaccg gtcgtttata tctgcgactc atagtatgac tcattaagcc taaaattaag
    20341 cttcccaaaa atgaagcgcc aacttttcca aactctaaat ctaccgttaa ttggtaaatc
    20401 gtccatgcaa tggcacctac aaatccacat gctactaaga ggcgtttggg tgcattgaaa
    20461 atgatagaga aaagtactgt tgatataaag ctgattgtaa aatgaaataa ataaaatagc
    20521 atgctttaac agtccttcct taaatgatta ataaaacgat tgcgacacca gcaccgattg
    20581 cgaatgctgt taatgcagct tcaacacctc gagacatacc tgcaagtaat tcacccgcta
    20641 ataaatctcg aatggcattg gtaattaata taccagggac aagtggcatg acactggcta
    20701 tagtaatgat atcttgattg gttgcaatgc ctaatttagt aaatgtggct gcaatggata
    20761 tgaccacagc agctgcaaca aactctgaga aaaatttaat ttgtatatag cgttgcacaa
    20821 agctgaatgt taaaaatgcg gatccaccag caatgactgc aatccaacaa tctgatgcga
    20881 caccaccaaa cataaatagg aagaagccac atgcaatggc agctgcaaag aaattcgtta
    20941 aaaaagaata ttgtaatgat gcatgctgta aatgaataaa ttcagattta gcttcatcaa
    21001 ttgtgagttc tttatttgat attttacgtg aaagactatt cgttaaagcg attttctcta
    21061 aatctgttgt acgctcttgt acacgaatta atcttgtact tgttcgatcg tttaatgaaa
    21121 aaataattgc agttgaactg acaaaactat atgtattatg aagaccataa ctatgtgcga
    21181 tacggttcat tgtatcttca actcgatatg tttcagcacc tgattcaagt aaaattctac
    21241 ctgcaattaa tacaacatca atcactttgt tttcatctat aattgtgatt gaatctggca
    21301 tatcaattca cctccaatga tatgtgttat ttatttgaac aattgaagtt tacaacttgt
    21361 tgttacaact ttcaatagtg agactttgtg ttagtatgat gaacttgtat ggttcaaatt
    21421 taaataagaa aaactgttaa tctttgctat tatactatga tttaataata gcaaaggatt
    21481 aacagttttg tcgttgttat aaattgataa tagggttaaa cattactttg tttcgccctt
    21541 gatttttggc tacatgcacc atatcgtctg catctttaaa cactttacgc tgtgattttg
    21601 gatcgtcatc tgttaaataa ccaacaccga tagacactga caatttaata acttctttgt
    21661 ttggtaaatg gaatgatgat ttttcaacac ccgaacgaat attttcagct aatttaacac
    21721 tttgatcaag tgaataattg tgaatgacaa ctgagaactc ttcgccacca tttctaaaaa
    21781 ttttaaattg attcggcaca tagtttttaa gtaattgaga catttgtttt aatacagcat
    21841 cacctgattt gtgtgagtag gtatcattga catctttaaa tccatcgata tcgattaata
    21901 ataaagcgat actttgatgt tctttttcag cttttcgtga aatttcattt aaatgtctat
    21961 caaattcttt tacattacct aagcctgtta agtaatcata tttatcttcg ttttcataac
    22021 gatttacgag tgagaagaaa tgccaaatat cgacaaatgt tatcgctgaa gctaaagtga
    22081 taattaatga aattggtatt aaaatgataa cttccgatag tgtataaata ggactcacta
    22141 acgcgacacc aaataaaatg attattgtaa caacattaag tattaataat gatagcacat
    22201 cattttgttt taaaaatggt ccaatagcac ttgttactgc agcaataaca atcaacgtaa
    22261 caccgtacat aatcgagttg ttaaatacta caatttcaac aattgctaca attactgtgg
    22321 cagataatgt atagaccata tttgtaaatc tacctaaaaa caataaagga acgaatgtta
    22381 agtgaattaa ataatcttca cgataaggga tagggtagac agataataat aatgatacga
    22441 ttgtcattaa aacagtgaca taagccttag aaaaaaccat acgtttgttt tctgaatact
    22501 gtaagcgatg gaataaatag attccagcga ctataacaga tatattgtat ataaatgctt
    22561 cgaacatgtc tgaatcgact cctttaattg accactagct attgtaagtg aaaacttaca
    22621 atttgtcatt agtttacata taaaattaat gtatgatata gactttgatg ttaaaatgtt
    22681 gccttaaatg atatgatgaa aaaatgaata ataggcgata tataagaaat gaatcgtata
    22741 gttgtaaata tgatatcatt gattgagcga attaatttat aataaagcta taagatatac
    22801 gtagaaaata gatatatcat tctataaaga caatattaaa taaatataac gttaaaaaca
    22861 attaatatcg atgaaggtga ataaatggtt acattattac tagttgcagt aacaatgatt
    22921 gtcagtttga cgataacacc aattgttatt gcaatatcga aaagattaaa tttagttgat
    22981 aaaccaaatt ttagaaaagt acacactaaa cctatttcag ttatgggtgg tacagtgatt
    23041 ctcttttcat ttttaatagg tatttggatt ggtcatccta ttgaaacaga aatcaaacca
    23101 cttattattg gtgcgattat tatgtacgta cttgggcttg tagatgatat ctacgatttg
    23161 aaaccgtata taaaattggc tggtcaaatt gccgctgcct tagtagttgc tttttatggt
    23221 gtgactattg attttatttc gttgccaatg ggtacaacga ttcattttgg atttcttagt
    23281 attccaatta ctgtgatttg gattgttgct attacaaatg caattaactt aattgatgga
    23341 ctcgatggtt tggcgtcggg tgtttctgca atcggactca ttacaatagg attcattgca
    23401 attttacaag ctaatatttt cataacgatg atttgttgtg ttttattagg ctctttaatt
    23461 gggtttttat tttacaattt ccatcctgcc aaaatatttt taggtgatag tggggcttta
    23521 atgattggat ttatcatcgg attcctttct ttactcggat tcaaaaatat tacaattatt
    23581 gcattgttct tcccaattgt tatcttagca gttccattca ttgatacttt gttcgcaatg
    23641 attcgacgtg tgaaaaaagg gcagcatata atgcaagctg ataaatcgca tttgcatcat
    23701 aaactattag ctttaggcta cacacataga caaacagtat tattaatcta ttcaatctct
    23761 attttattta gtctttcgag cattattttg tatgtatcgc caccattagg tgttgtatta
    23821 atgtttgtat taatcatatt tagtattgaa ttaattgttg aatttacagg attaatagat
    23881 aacaactacc gaccaatatt aaatttaatt agtcggaaat cgtcacacaa agaagattag
    23941 attttaaaac agtaagtaat aaaataaaaa aatgatgtct gtacttttat gttgttataa
    24001 aggaagacat actcacaaaa aacactagca tggaacgtgc tttccaaatt gcaaagtaac
    24061 aatacgcaat tagagttagc cctcctaagc tagtgttttt atttttattc agttggtata
    24121 tcgaaaggta actgctttgg agtttcttca gtcaaatcga aatttccagc agtcatttga
    24181 tttaaaaagt taataaacgc ttcatagtca cttttaacga catcgatata gtagcttacc
    24241 ttatcagtgt aagtttggtt tcttaacata aaatgagttg aagctaattc atattcaaat
    24301 ttaccagttt gatcataatt cagtgttact atacatggta ctgcttctcg tagttcgaca
    24361 cgcccgacat cataaatgac gtctctaaca gcaccgctat aggcgcgaat taaaccgcca
    24421 ccacctaatt taataccacc aaaatatctt gttactacga cacacgcatt atgaacatcg
    24481 agctttttta atatgtctaa cattgggaca ccggcagttc ctgtcggttc accatcatca
    24541 ttcgcttttt gaatattcat ttcaggtcca atagtatatg cagaacaatt atgagtggca
    24601 tctttatgtt ctttttttat tgcagcaata aatgctttag cttcatcttc attttgaaca
    24661 ggtttgatat gagcaatgaa tcttgattta ctaatcacat tttcaataat gtgttctttt
    24721 ttaacagtaa tgatattttg tgtcataata actccttaat tcataagctt aagattattt
    24781 aatcttcatt atacactgaa aatgacatga ctataaatcg tttgattgcc attttctttt
    24841 taactgaaat attgtatcat tgctatgagt atattttagg aggacgacta tgaaaattgc
    24901 tgtgatgacc gattctacaa gttatctgtc gcaggactta atcgataaat ataatattca
    24961 aatagcgcca ttaagtgtga cttttgatga tggaaagaac tttacagaaa gtaatgaaat
    25021 agcaattgaa gaattttata ataaaatggc atcgtctcaa acgattccga caacaagcca
    25081 accagcaatt ggcgaatgga ttactaaata tgaaatgcta agagatcagg gttacacaga
    25141 tatcattgtc atttgcttat ccagtgggat tagtggaagt tatcaatcta gttatcaagc
    25201 aggggaaatg gttgaaggtg ttaatgtaca tgcatttgat agtaagcttg cagcaatgat
    25261 tgaaggatgt tatgtattac gtgctattga aatggttgaa gaaggatacg aaccacagca
    25321 aattattgat gatttaacta atatgcgtga gcacacaggt gcatatttaa ttgttgatga
    25381 cttaaagaat ttacaaaaaa gtggtcgaat tactggtgct caagcatggg ttggaacatt
    25441 attgaaaatg aagccagttc ttaagtttga agatggcaag attataccag aagaaaaagt
    25501 tcgtactaaa aagcgtgcca ttcaaacatt agaaaagaaa gtattagata ttgtaaaaga
    25561 ctttgaagaa gtaactttat ttgtcataaa tggagatcat tttgaagatg gtcaagcgtt
    25621 atacaaaaag ttacaagaag attgtccttc aggttatcaa gtagcatact ctgagtttgg
    25681 tccagttgtt gcagcacatt taggttctgg tggattaggt ttaggctatg ttggcagaaa
    25741 aataagatta acataattat aaaattttaa taaaagagtc tatattgtaa ttggaaatta
    25801 tctctcgtat acatggcttt aaatgttcat catttgaaag tcaaaatgct aaagatataa
    25861 gaaaatcatt ataatattag gctctttttt acgttgaaat gaggttttaa gcattaaaca
    25921 ttacgggaaa ttaattcatc ctcatacttc acttactaat gaaaaaatta aaaaagaagt
    25981 aacaggtgtc atcaaacaaa attcaaacta ttattgtgtt caatgtgaaa gtacaaatcc
    26041 aaagcatttt tatcagtatg attcctcagt acattccaag aaaattgtat attgcagaaa
    26101 ttgtatatca ctgggtcgaa tggataatgt aacaagatat aaaataacag agagttcgca
    26161 aagttcatca caagcatatt atcatctctc atttgaattg tcggaacagc agtcttatgc
    26221 ctcagaacat attgttcgag ccattagaat gagacaaacg attttgttat atgccgtaac
    26281 aggtgcaggt aagacagaaa tgatgtttca aggcattcaa tatgcaagac gacagggaga
    26341 taatatagct attgtgtcac cgcgtgtaga tgttgttgta gaaattagta aacgtattaa
    26401 agacgcattt cttaatgaag atatagacat actacaccag caatcaagtc aacaatttga
    26461 agggcatttt gttgtatgca cagtacatca actttaccga ttcaaacagc actttgatac
    26521 tatttttatt gatgaagtcg atgcctttcc tttatcaatg gataaaagtt tacaacaagc
    26581 attgaagtca tcttctaaag ttgaacatgc aacgatttat atgacagcaa caccaccgaa
    26641 acaacttctg tcagagattc cccacgaaaa tataattaaa ttgccagctc gctttcataa
    26701 aaaatcactt ccagttccta aatatcgtta tttcaaactt aataataata agattcagaa
    26761 aatgttatac cgaattttac aagatcaaat taataatcaa cgttatacac tggtgttttt
    26821 taacaatata gaaacaatga ttaaaacatt ttcggtttat aagcagaaaa ttactaaatt
    26881 aacatacgtc catagcgagg atgtttttcg ctttgaaaaa gttgaacaat taaggaatgg
    26941 acatttcgat gtcattttta ctacgacaat attagaacgt ggatttacaa tggcaaattt
    27001 ggatgttgtt gttatcgatg cacatcaata tactcaagag gctttaatac aaattgctgg
    27061 acgtgttgga cgaaaattag aatgtcctgc tggaaaagta ttgttttttc atgaaggagt
    27121 aagtatgaat atgattcaag ctaaaaaaga gattcaaagg atgaacaaat tagcattaaa
    27181 aagaggttgg attgatgaat aattgtttga gttgtggtgc taagttatat gaaaatataa
    27241 ccatttataa tttgttcaag aaacctaata gattatgtga cagatgcaaa gagaattggg
    27301 acaatattaa acttgatatt aaagcaaggc gatgttcaag gtgcttaaaa cacttaaatc
    27361 aagatgaagc gtattgttta gactgcaagt ttctatcggc acactttaat ttaatggaac
    27421 aattatattg tcaatttcaa tatgacggtt taatgaaaga gatgatacat cagtataaat
    27481 ttttgaaaga ctattattta tgtgaattat tggcacattt gattgaaata ccacaaacat
    27541 cttatgacta tattgtgcca attccttctt cgccggcaca tgatttatct agaacattta
    27601 acccggtaga agcagtacta aaagctaaag ggattcgctt tgataagatt ttaaagatgt
    27661 caaatagacc aaaacagtct catttaacta agaaagagcg tctggcagat gaaaatccat
    27721 ttattattga tacggaatta gatttaaatg gcaaggaaat attactcgtt gacgatattt
    27781 atacaactgg attaacaatt catcgtgcag ggtgtaaatt atatgctaaa aatatcagaa
    27841 aattcaaagt gtttgcgttt gcacgatagc gtaaaaatgt taaaatataa taaagagtta
    27901 ccaataaaga ggtttaagga gagattacta tgattagatt tgaaattcat ggagataacc
    27961 tcactatcac agatgctatt cgcaactata ttgaggaaaa aattggtaag ttggaacgtt
    28021 attttaatga cgtaccaaat gcagtggcgc atgttaaagt taaaacttat tcaaattcag
    28081 ctactaaaat tgaagtaaca attccattga aaaatgttac gttaagagct gaagagcgaa
    28141 acgatgattt atacgcaggt attgatttaa ttaataataa acttgaaaga caagttcgaa
    28201 aatataaaac acgtattaat cgtaagagcc gtgatcgagg agatcaagaa gtgtttgttg
    28261 ccgaattaca agaaatgcaa gaaacacaag ttgataatga cgcttacgat gataacgaga
    28321 tagaaattat tcgttcaaaa gaattcagct taaaaccaat ggattcagaa gaagcggtat
    28381 tacaaatgaa tctattaggt catgacttct ttgtattcac agacagagaa actgatggaa
    28441 caagtatcgt ttaccgacgt aaagacggta aatatggctt gattcaaact agtgaacaat
    28501 aaattaagtt taaagcactt gtgtttttgc acaagtgctt ttttatactc caaaagcaaa
    28561 ttatgactat ttcatagttc gataatgtaa tttgttgaat gaaacatagt gactatgcta
    28621 atgttaatgg atgtatatat ttgaatgtta agttaataat agtatgtcag tctattgtat
    28681 agtccgagtc gaaaatcgta aaatatttat aatataattt attaggaagt ataattgcgt
    28741 attgagaata tatttattag tgataaactt gttgacaaca gaatgtgaat gaagtatgtc
    28801 ataaatatat ttatattgat tccacaaatg agtaaataag tataattttc taactataaa
    28861 tgataagata tattgttgta ggccaaacag ttttttagct aaaggagcga acgaaatggg
    28921 atttttatca aaaattcttg atggcaataa taaagaaatt aaacagttag gtaaacttgc
    28981 tgataaagta atcgctttag aagaaaaaac ggcaatttta actgatgaag aaattcgtaa
    29041 taaaacgaaa caattccaaa cagaattagc tgacattgat aatgtcaaaa agcaaaatga
    29101 ttatttagat aaaattttac cagaagcata tgcacttgtt agagaaggct ctaaacgtgt
    29161 attcaatatg acgccatata aagttcaaat tatgggtggt attgcaattc ataaaggtga
    29221 tatcgctgag atgagaacag gtgaaggtaa aacattaaca gcgacaatgc caacatactt
    29281 aaatgcatta gctggtagag gtgttcacgt tattacagtc aatgaatact tatcaagtgt
    29341 tcaaagtgaa gaaatggctg agttatataa cttcttaggt ttgactgtcg gattaaactt
    29401 aaacagtaag acgacagaag aaaaacgtga agcatacgca caagacatta cttacagtac
    29461 taataatgag ctaggttttg attacttacg agataacatg gtgaattatt ctgaagatag
    29521 agtaatgcgt ccattacatt ttgcaatcat tgatgaggtt gactcaattt taatcgacga
    29581 ggcacgtacg ccattaatta tttctggtga agctgaaaag tcaacgtcac tttatacaca
    29641 agcaaatgtt tttgcgaaaa tgttaaaaca ggacgaagat tataaatacg atgaaaaaac
    29701 gaaagccgta catttaacag aacaaggtgc ggataaagct gaacgtatgt tcaaagttga
    29761 aaacttatat gatgtacaaa atgttgatgt tattagtcat atcaacacag ctttacgtgc
    29821 gcacgttaca ttacaacgtg acgtagacta tatggttgtt gatggcgaag tactaattgt
    29881 cgatcaattt acaggacgta caatgccagg ccgtcgtttc tcagaaggtt tacaccaagc
    29941 tattgaagcg aaggaaggcg ttcaaattca aaatgaatct aaaactatgg cgtctattac
    30001 attccaaaac tatttcagaa tgtacaataa acttgcgggt atgacaggta cagctaaaac
    30061 tgaagaagaa gaatttagaa atatttataa catgacagta actcaaattc cgacaaataa
    30121 acctgtgcaa cgtaacgata agtctgattt aatttacatt agccaaaaag gtaaatttga
    30181 tgcagtagta gaagatgttg ttgaaaaaca caaggcaggg caaccagtgc tattaggtac
    30241 tgttgcagtt gagacttctg aatatatttc aaatttactt aaaaaacgtg gtatccgtca
    30301 tgatgtgtta aatgcgaaaa atcatgaacg tgaagctgaa attgttgcag gcgctggaca
    30361 aaaaggtgcc gttactattg ccactaacat ggctggtcgt ggtacagata tcaaattagg
    30421 tgaaggcgta gaggaattag gcggtttagc agtaataggt acagaacgac atgaatctcg
    30481 tcgtattgat gaccagttac gtggtcgttc tggacgtcaa ggtgataaag gggatagtcg
    30541 cttctattta tcattacaag atgaattaat gattcgtttt ggttctgaac gtttacagaa
    30601 aatgatgagc cgactaggtt tagatgactc tacaccaatt gaatcaaaaa tggtatcaag
    30661 agctgtagaa tcagcacaaa aacgtgtaga aggtaataac ttcgacgcgc gtaaacgtat
    30721 cttagaatac gatgaagtat tacgtaaaca acgtgaaatt atctataacg aaagaaatag
    30781 tattattgat gaagaagaca gctctcaagt tgtagatgca atgctacgtt caacgttaca
    30841 acgtagtatc aattactata ttaatacagc agatgacgag cctgaatatc aaccattcat
    30901 cgactacatt aatgacattt tcttacaaga aggtgacatt acagaggatg atatcaaagg
    30961 taaagatgct gaagatattt tcgaagtcgt ttgggctaag attgaagcag catatcaaag
    31021 tcaaaaagat atcttagaag aacaaatgaa tgagtttgag cgtatgattt tacttcgttc
    31081 tattgatagc cattggactg atcatatcga cacaatggat caattacgtc aaggtattca
    31141 cttacgttct tatgcacagc aaaatccatt acgtgactat caaaatgaag gtcatgaatt
    31201 atttgatatc atgatgcaaa atatcgaaga agatacttgt aaattcattt taaaatctgt
    31261 agtacaagtt gaagataata ttgaacgtga aaaaacaaca gagtttggtg aagcgaagca
    31321 cgtttcagct gaagatggta aagaaaaagt gaaaccgaaa ccaatcgtta aaggcgatca
    31381 agttggtcgt aacgatgatt gtccatgtgg tagtggtaaa aaattcaaaa attgccatgg
    31441 aaaataaatg atataaaata actccttcca attaaacacc tatagtttgt gttatgggag
    31501 gagtcttttt attttacaag cgttaaatac tttaaaaaat gtgaagaagt tgttaaacgt
    31561 tgttatgtac ttagttttaa aaaatcggtt taggcatatg tcgatgataa atgtactgat
    31621 ttttaacaat aaaagcataa actaattgtc agtgtgctta tatttcttaa cattgttatt
    31681 taacaaaatt atgttaaaat ttagcattat aaaagataca aatcaatgac ttgaattgaa
    31741 ataggagcga aggctatgga attatcagaa atcaaacgaa atatagataa gtataatcaa
    31801 gatttaacac aaattagggg gtctctttga cttagagaac aaagaaacta atatccaaga
    31861 atatgaagaa atgatggcag aacctaattt ttgggataac caaacgaaag cgcaagatat
    31921 tatagataaa aataatgcgt taaaagcaat agttaatggt tataaaacac tacaagcaga
    31981 agtagatgac atggatgcta cttgggattt attacaagaa gaatttgatg aagaaatgaa
    32041 agaagactta gagcaagagg tcattaattt taaggctaaa gtggatgaat acgaattgca
    32101 attattatta gatgggcctc acgatgccaa taacgcaatt ctagagttac atcctggtgc
    32161 aggtggcacg gagtctcaag attgggctaa tatgctattt agaatgtatc aacgttattg
    32221 tgagaagaaa ggctttaaag ttgaaactgt tgattatcta cctggggatg aagcggggat
    32281 taaaagtgta acattgctca tcaaagggca taatgcttat ggttatttaa aagctgaaaa
    32341 aggtgtacac cgactagtac gaatttctcc atttgattca tcaggacgtc gtcatacatc
    32401 atttgcatca tgcgacgtta ttccagattt taataatgat gaaatagaga ttgaaatcaa
    32461 tccggatgat attacagttg atacattcag agcttctggt gcaggtggtc agcatattaa
    32521 caaaactgaa tcggcaatac gaattaccca ccacccctca ggtatagttg ttaataacca
    32581 aaatgaacgt tctcaaatta aaaaccgtga agcagctatg aaaatgttaa agtctaaatt
    32641 atatcaatta aaattggaag agcaggcacg tgaaatggct gaaattcgtg gcgaacaaaa
    32701 agaaatcggc tggggaagcc aaattagatc atatgttttc catccatact caatggtgaa
    32761 agatcatcgt acgaacgaag aaacaggtaa ggttgatgca gtgatggatg gagacattgg
    32821 accatttatc gaatcatatt taagacagac aatgtcgcac gattaatata tattttaaaa
    32881 ccgaggctct aaaagggcgt cggtttttgg tttttttaaa ggtagctaaa taaattgtaa
    32941 attagatttt ggaatatgat ttgtttatga atatttaagt acaattcggt agatagagtt
    33001 agaatatatt ttttaaaagt tgtgtttgtt aaaacaacat atgcagtgtg catttagtaa
    33061 tattacctat ggcgattttc aaggtattga attaatcgtt gaaaacgttc tcaattacat
    33121 ggtatgaata cattttacac tatgataaaa ggttgtattc tttttatatt gttaaccatt
    33181 tgattacatc gttataacaa tagcttttga caaaatgtat tgtgctatag tatttgcata
    33241 cttaaaatac taacagcaaa ggaatgacag caagatgaaa aaaactctta cagtgacggt
    33301 ttcgtcagtg ttagcttttt tagctttaaa taatgcagca catgcacaac aacatggcac
    33361 acaagtaaaa acacctgttc aacataacta tgtatcaaat gttcaagcac aaacgcaatc
    33421 accgacaact tatacagtag ttgctggcga ttcattatat aagattgctt tagagcatca
    33481 cttaacgttg aatcaattat attcatacaa tcctggtgta acacctttaa tttttcctgg
    33541 tgacgtgatt tcacttgtgc ctcaaaataa agtgaaacaa actaaagcgg ttaaatcacc
    33601 agtaagaaaa gcaagccaag ctaaaaaggt agtaaaacaa cctgtacaac aagcgtctaa
    33661 aaaagtagta gttaagcaag cacctaagca agcagtagct aagacagtta atgtagcata
    33721 taaacctgct caagtacaaa aatcagtacc aactgtacct gttgcacata actacaataa
    33781 atcagttgct aacagaggaa acttatatgc ttatggaaac tgcacatatt atgccttcga
    33841 tcgtcgtgca caattaggta gaagtatagg aagtttatgg ggcaatgcaa ataactggaa
    33901 ttacgcagca aaagttgcag gatttaaagt agataaaaca ccagaagttg gcgctatttt
    33961 ccaaacagct gctggcccat atggacatgt tggtgttgtt gaatctgtaa accctaatgg
    34021 aacaattact gtttcggaaa tgaactatgc tggatttaat gttaaatctt caagaacaat
    34081 tttaaatcca ggaaaatata attacatcca ctaagtaata tatcaagaca agactatcct
    34141 cttagcctgt ttaagtaaca ggttgagagg attttttggt atcatttaat cagagttata
    34201 taaagaagat atttaaatga tatttaattg attgatatgt aaaaagaaag tataatttat
    34261 gattaattaa tggaggaggt aattgaaatg ggtgtacatc aatattttaa aagattatca
    34321 gatatggaaa gacttataag attacctgga aaatttaaat attttgaaca caatgttgca
    34381 gcacactcct ttaaagtaac taaaattgct caatatctag caacagttga agaatatcat
    34441 ggtcgaaaga ttaattggaa aagcttatat gaaaaagcat taaatcatga tttcgctgaa
    34501 gtgtttactg gtgatataaa aacacctgtt aaatatgcga gtagtgaatt aaaaaaatta
    34561 ttttcgcaag ttgaagaaga aatggtagag acctttattg aagaagaaat tccattacaa
    34621 tatagagatg tttataagca acgactgcaa gaaggtaaag atgattcatt agaaggccaa
    34681 atactttcag ttgctgataa aattgatttg ctttatgaaa catttggaga aatacaaaaa
    34741 cgtaatcccg aagaattatt tttcgaaatt tatgaaatga gtctagaaac aattattcaa
    34801 tttgaccatt tagcatctgt acaagatttt attaataata tcattccaga aatgttgact
    34861 gaaaacttta tacctagaac agaattaaga gaaacaacca tgaacatttt aaataaaaga
    34921 aaagaggaaa atgaatgata tggtatttta gcgcagcatt ctttccatgt gtcttggtag
    34981 tattatttag tgtaataaca agaagtaaat gggtcggtac tattctgaca ttaattttaa
    35041 ttggtgcctc aatctataaa gagtatttcc ataacgagtg gattattttt attgatgtag
    35101 tgtcattatt agctggttat ttaattatag atcaactcga atttcataaa catcaagatg
    35161 aagatcgcta agattaactt taaaataatg tttcaaacaa atttgttgaa acaaaacgat
    35221 gattaatata atgtgtattt acatactaaa aataacaaga taaacgattt gatttaaggc
    35281 aagcatagtt agcacaacta tgtttgtttt tctttgttcg acatttttac gaacaaacgt
    35341 ttgctttttg tgtggctact ttgctaaaat atgtaatgag aaagcgaaga gttgagcgga
    35401 aatataaata atacgttgaa agaggaggca tatgtgacaa tggttgaaca ttatcctttt
    35461 aaaatacatt ctgattttga gcctcaaggt gaccaaccgc aagcaattaa ggaaatcgtg
    35521 gatggtatta aagcggggaa aagacatcaa actttattag gtgcaactgg cacagggaaa
    35581 acatttacga tgagtaatgt tattaaagaa gttgggaaac caacgttaat tatcgcacat
    35641 aacaaaacat tagcagggca attatatagt gagtttaaag aattttttcc tgaaaacagg
    35701 gtggaatact ttgtaagtta ctatgattat tatcaacctg aggcatacgt accgtctact
    35761 gacactttta ttgaaaaaga tgcctcaatc aatgatgaaa ttgatcaact acgacattct
    35821 gctacaagtg cattatttga acgcgatgat gtaattatta ttgctagtgt aagttgtata
    35881 tatggtttag gtaatcctga agaatataaa gatttagtag taagtgttcg agttggtatg
    35941 gaaatggata gaagtgaatt acttagaaaa cttgtagatg tgcaatatac acgaaatgac
    36001 atcgatttcc aacgaggaac gtttcgagtg cgtggtgatg tagttgaaat attcccagcc
    36061 tctaaagaag aactttgtat aagggttgag tttttcggcg atgagattga ccgtatccga
    36121 gaagttaact acctaacagg tgaagtgttg aaagaaagag aacattttgc gatattccca
    36181 gcttctcact tcgtaacacg tgaagaaaag ttgaaagttg cgattgaacg tattgaaaaa
    36241 gaattggaag aacgattgaa agaattacga gatgagaata aattactaga agcgcaaagg
    36301 ttagaacagc gtaccaacta tgatttagaa atgatgcgag agatgggatt ctgttcagga
    36361 attgaaaact attccgtaca tttaactttg cgaccactgg gttcgacacc gtatacttta
    36421 ttggattact ttggcgatga ttggttagta atgattgatg aatcacatgt gacattacca
    36481 caagttcgag gcatgtataa cggagacaga gcgcgtaaac aagttttggt ggatcatgga
    36541 tttagattac cgagtgcatt agataaccgt ccacttaaat ttgaagaatt tgaagaaaag
    36601 acaaaacaac ttgtgtatgt atctgcaacg cctggaccat acgaaattga acatacggat
    36661 aagatggttg aacaaattat tcgtcctact ggtttactgg atcctaaaat tgaggttaga
    36721 cctactgaaa atcaaattga cgatttatta agtgaaattc aaacaagggt tgagcgtaat
    36781 gaacgcgtac ttgttacaac gctcactaaa aagatgagtg aagatttaac cacatacatg
    36841 aaagaagcgg gtattaaagt taattatctg cattcagaaa tcaagacatt agaacgaatc
    36901 gaaataatta gagacttacg aatgggtaca tatgatgtta tcgtaggtat taatttatta
    36961 agagagggta ttgatatacc agaagtttct ctagttgtca tattagatgc agataaagaa
    37021 ggatttttac gttctaaccg ctcattaatt caaacaatag gtagagctgc gcgtaacgat
    37081 aaaggtgaag tcattatgta tgccgataaa atgactgatt cgatgaagta tgcaattgat
    37141 gagacacaac gtcgtcgaga aatacagatg aaacataatg aaaaacatgg tattacacct
    37201 aaaacaatta ataaaaaaat acatgattta attagtgcta ctgttgaaaa tgacgaaaat
    37261 aatgacaaag cacaaactgt gatacctaag aagatgacga aaaaagaacg tcaaaagaca
    37321 atcgacaata tagaaaaaga aatgaaacaa gcagcgaaag atttagattt cgagaaagct
    37381 acagaattaa gagatatgtt atttgaatta aaagcagaag ggtgacaagt aaatgaaaga
    37441 accatccata gtagtaaaag gtgctcgtgc gcataacttg aaagatattg atatcgaact
    37501 acctaaaaat aaattaattg ttatgacagg tttatctggg tcaggtaaat cgtcattagc
    37561 attcgatact atatatgctg aaggacaacg acgttatgtt gaatcattaa gtgcctatgc
    37621 gcgtcaattt ttaggccaaa tggacaaacc agatgttgat acaattgaag gattatcgcc
    37681 agcaatttca atagatcaaa aaacaacaag taaaaatcca agatcaactg tagcaacagt
    37741 aacagaaata tatgattata tacgtttgtt atatgcacgt gttggtaaac cttactgtcc
    37801 aaatcacaat atagaaattg aatcgcaaac agtacaacaa atggttgacc gcattatgga
    37861 attagaggca cgtacaaaga ttcaattatt agcacctgtc atcgctcatc gtaaaggtag
    37921 tcatgaaaag ctaatcgaag atattggtaa aaaaggttat gtacgtttaa gaatcgatgg
    37981 cgaaattgtt gatgtaaatg atgtacctac tttagataag aacaagaatc atacaataga
    38041 agttgttgta gaccgattag ttgttaaaga tggaattgaa acacgactag ctgactctat
    38101 agaaactgcg ttagagcttt cagaaggaca attaacagtt gatgtcattg acggggaaga
    38161 ccttaagttt tcagaaagcc atgcttgtcc tatatgtgga ttttcaatcg gagagttaga
    38221 accaagaatg tttagcttta acagtccttt tggtgcttgt ccgacatgtg atggcttagg
    38281 ccaaaagtta acagtcgatg tagacttggt tgttcccgac aaagataaga cgctaaacga
    38341 aggtgcaata gaaccttgga taccgacgag ttctgatttt tatccaacat tgttaaaacg
    38401 tgtttgtgaa gtttataaaa tcaatatgga taaacctttt aaaaagttaa cagaacgtca
    38461 acgtgatatt ttattgtatg gttctggtga caaagaaatt gaatttacat ttacacaacg
    38521 tcaaggtggt actagaaaac gaacaatggt tttcgagggt gtagttccta atataagtag
    38581 acgattccat gaatctcctt cagaatatac acgtgaaatg atgagtaaat atatgaccga
    38641 actaccttgt gaaacttgtc atggaaagcg attgagtcgt gaagcgttat ctgtttatgt
    38701 aggtggttta aatattggtg aagtagtcga atattcaatc agtcaagcgc tgaactatta
    38761 taaaaacatt gatttgtcag aacaagatca agcgattgca aatcaaatat tgaaagaaat
    38821 tatttcccga ctcacttttt taaataatgt gggacttgaa tatttaacgc taaacagagc
    38881 ttcaggtaca ctttcaggtg gtgaagcaca acgtattcga ttggcaacgc aaattgggtc
    38941 gcgtttgact ggtgtcttat atgtattaga tgagccatca attggactgc atcaaagaga
    39001 taatgatcga ttaattaata cacttaaaga aatgagagat ttaggaaata ctttaattgt
    39061 agttgaacac gatgatgata caatgcgtgc ggctgattac ttagtggata tagggcctgg
    39121 tgctggtgaa catggaggac aaattgtgtc tagtgggaca cctcaaaagg taatgaaaga
    39181 taaaaaatca ttaacaggac aatacttgag tggtaagaaa cgtattgacg tacctgaata
    39241 tcgcagaccg gcttcagatc gtaaaatttc tatacgtgga gctagaagca acaatcttaa
    39301 agggattgat gtggacatac cactatcaat catgacggtt gttacaggtg tatcaggttc
    39361 tggtaaaagc tcattagtaa atgaagtatt atacaaatca ttagctcaaa aaattaataa
    39421 atctaaagta aagccaggat tgtacgataa gattgaaggt attgatcaac ttgataaaat
    39481 tattgatatt gatcaatcac cgataggtag aacgccacgc tctaatccag caacatatac
    39541 tggtgtgttt gatgatatac gtgatgtgtt tgcgcaaaca aatgaagcta aaattcgagg
    39601 atatcaaaaa gggcgtttta gttttaatgt aaaaggtgga cgctgtgaag cttgtaaagg
    39661 tgacggtatt attaaaattg aaatgcattt tttacctgat gtttatgttc cttgtgaagt
    39721 gtgtgatggt aaacgatata atcgtgagac actagaggtt acttacaaag gtaaaaatat
    39781 tgctgacatt ttagaaatga ctgttgaaga agcaacacaa ttttttgaaa atattcctaa
    39841 gattaagcgc aagttacaaa cactagttga tgttggtctt ggatacgtca cattaggtca
    39901 acaagctaca acgttatcag gtggtgaggc tcaacgtgtg aaacttgcat ctgaacttca
    39961 taaacgttca actggtaaat ctatttatat cctagacgaa ccaacaacag ggttacatgt
    40021 tgacgatatt agcagattat taaaagtatt aaatcgatta gttgaaaatg gtgacactgt
    40081 tgtaattatt gaacataacc tagatgttat caaaacagca gactatatta tcgacttagg
    40141 tcctgaaggt ggtagtggcg gtggtactat tgttgcgact ggcacacccg aagatattgc
    40201 tcagacaaag tcatcatata caggaaagta tttaaaagaa gtacttgaac gagataaaca
    40261 aaatactgaa gataaataag attaaaagaa gtgaagggtg ttgtaaattt acccttcact
    40321 tctttttatt aatttagtaa tgaatagtag aaagaaaaga tgcgtaaaaa gaattatgtt
    40381 aagatagggt caatctagag ttgctaaaca taaatcgaaa ctgagagtgg gacagaaatg
    40441 ataaagaatc actaatgatt tattatgtag tggttctttg tcattagcca cagctattgt
    40501 gtacttaaaa ataggaatgc atgagtgcaa ctcatgcata agaaatacta atttctaa
//
LOCUS       DAOBBH010000004        37532 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.004, whole genome shotgun sequence.
ACCESSION   DAOBBH010000004 DAOBBH010000000
VERSION     DAOBBH010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 37532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37532
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.004"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..202
                     /locus_tag="P6L01_000118"
     CDS             <1..202
                     /locus_tag="P6L01_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HDP3029221.1"
                     /translation="QYLVTTFKDSTGQPHEHFTTARDNQTFTVVEAESKEEAKEKYEA
                     QVKRDAIIKLGQLFENIRECRK"
     gene            199..393
                     /locus_tag="P6L01_000119"
     CDS             199..393
                     /locus_tag="P6L01_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029222.1"
                     /translation="MTDVKIKTISGGVYFVKTAEPFEKYVERMTSFNGYIYASTIIKQ
                     PTYIKTDTIESITLIEERGK"
     gene            390..593
                     /locus_tag="P6L01_000120"
     CDS             390..593
                     /locus_tag="P6L01_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029223.1"
                     /translation="MNQLRILLHDGSSLILHEDELFNEIVFVLDDFRNDDDYLTIEKD
                     YGRELVLNKGYIVGINVEEADDD"
     gene            586..759
                     /locus_tag="P6L01_000121"
     CDS             586..759
                     /locus_tag="P6L01_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator RinB"
                     /protein_id="HDP3029224.1"
                     /translation="MTKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEMPSDFAKL
                     SDQSDLMRAEVSE"
     gene            760..864
                     /locus_tag="P6L01_000122"
     CDS             760..864
                     /locus_tag="P6L01_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029225.1"
                     /translation="MMWLVIAIILLVILLFGVMLQAEQLKGDVKVKER"
     gene            930..1352
                     /locus_tag="P6L01_000123"
     CDS             930..1352
                     /locus_tag="P6L01_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001627805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RinA family phage transcriptional activator"
                     /protein_id="HDP3029226.1"
                     /translation="MTKKKYGLKLSTVRKLEDELCDYPNYHKQLEDLRSEIMTPWIPT
                     DTNIGGEFVPSNTSKTEMAVTNYLCSIRRGKILEFKSAIERIINTSSRKEREFIQEYY
                     FNKKTLIAVCYDIHISESTAHRIKKKIVSKLAEELGEY"
     gene            1540..2034
                     /locus_tag="P6L01_000124"
     CDS             1540..2034
                     /locus_tag="P6L01_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDP3029227.1"
                     /translation="MNKLTKKQRLFAEVYTIPGTECYGNATKSAVHAGYSEKTAYSQG
                     QRMLKNVEIQNYIKEVETKLFDENIMSGKEVLYRLTRTARGEHTEVEAVVTKTGDYKE
                     NPDTGKMQLVYDEHIQLVTKSPKISDQNKALEMLGRHHKLFTDKQEVDHKIPMFVDNI
                     PEDD"
     gene            2037..3332
                     /locus_tag="P6L01_000125"
     CDS             2037..3332
                     /locus_tag="P6L01_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDP3029228.1"
                     /translation="MYEILDLKNKIGGGYNKFWHNKNFYRVVKGSRGSKKSKTTAINL
                     IYRIMKYDWANILVVRRFSNTNKQSTYTDLKWATNQLGVAHLFKFNESLPEITYKPTG
                     QKILFRGLDDPLKITSITVDTGILCWAWFEEAYQIETFAKFSTVVESIRGSYDSPEFF
                     KQITVTFNPWSERHWLKPTFFDEETKLNNTFSDTTTYRVNEWLDKVDIERYEDLYIKN
                     PRRARIVCDGDWGVAEGLVFDNFKVEDFDWFEEFKRTQEITHGMDFGFSQDPTTVVST
                     VVDLKNKKLFIYDEHYKKAMLTDDIKQMLIKKGLGDVDIAADYGAGGDRVISELKSKG
                     IKGIRKALKGANTILPGIQFIQGFEVIIHPSCEHAIEEFNTYTFDQDNDGKWLNKPID
                     ANNHIIDALRYSLEKYHIVRKKRKKNIESKTKVIKSLGL"
     gene            3343..4881
                     /locus_tag="P6L01_000126"
     CDS             3343..4881
                     /locus_tag="P6L01_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDP3029229.1"
                     /translation="MLKANEFETDTDLRENRNYLFNDEANVVYTYDGTESDLLQNINE
                     VSKYIEHHMDYQRPRLKVLSDYYEGKTKNLVELTRRKEEYMADNRVAHDYASYISDFI
                     NGYFLGNPIQCQDDDKDVLEAIEAFNDLNDVESHNRSLGLDLSIYGKAYELMIRNQDD
                     ETRLYKSDAMSTFVIYDNTIERNSIAGVRYLRTKPIDKTDEDEVFTVDLFTSHGVYRY
                     LTSRTNGLKLTPRENGFESHSFERMPITEFSNNERRKGDYEKVITLIDLYDNAESDTA
                     NYMSDLNDAMLLIKGNLNLDPVEVRKQKEANVLFLEPTVYENRDTGIETEGSVDGGYI
                     YKQYDVQGTEAYKDRLNSDIHMFTNTPNMKDDNFSGTQSGEAMKYKLFGLEQRTKTKE
                     GLFTKGLRRRAKLLETILKNTRSIDANKDFNTVRYVYNRNLPKSLIEELKAYIDSGGK
                     ISQTTLMSLFSFFQDPELEVKKIEEDEKESIKKAQKGIYKDPRDINDDEQDDDTKDTV
                     DKKE"
     gene            4888..5883
                     /locus_tag="P6L01_000127"
     CDS             4888..5883
                     /locus_tag="P6L01_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDP3029230.1"
                     /translation="MPNKNTQEYWEERGRKAIENELKRDKTKAEEIERILNMMIKRIE
                     KEINAFIVKYGDFAGVTLQEAQKIIDEFDVKAFQEEAKRLVENKDFSDRANEELKKYN
                     TKMYVSREQMLKIQIEFLIAYATAQTELSMREYFESTAYRVFSDQAGILGEGVQVAKE
                     VIDTIVDTQFHGVVWSERLWTNTEAMKQEVEEIIANVVIRGRHPNEYVKDMRKHLNKF
                     EGTARQKTAAIKSLLYTESARVHAQSSIDSMKEISPEGYYMYIAKIDNRTTKVCKGLN
                     GEVFKVKDAKIGVNFYPMHINCRSDCALLPKSMWPKKQNKKRKTKYFGGKVKSGD"
     gene            5965..6138
                     /locus_tag="P6L01_000128"
     CDS             5965..6138
                     /locus_tag="P6L01_000128"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029231.1"
                     /translation="MSNTDKYLRDIARELKCIRKELQKRNEMIEDNNQKLEESLGKLF
                     TELTGSNMEIKLQ"
     gene            6270..6884
                     /locus_tag="P6L01_000129"
     CDS             6270..6884
                     /locus_tag="P6L01_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HDP3029232.1"
                     /translation="MDIQEKLKLKLQFFAEESDGDNGKSKDNNDDEGKDKQDKKTNSE
                     EEIEKRLQEEYNKRLKEELSRRMKQKEKEKQEAVDEAKRLAKMNKDQIAEYEREQMEK
                     ELEQLRSEKQLNEMRSEARKMLSEAEVDSSDEVVNLVVTDTAEQTKLNVEAFSNAVKK
                     AVNEAVKVNARQSPLTGGDSFNHSTKNKPQNLAEIARQKRIIKN"
     gene            6898..7872
                     /locus_tag="P6L01_000130"
     CDS             6898..7872
                     /locus_tag="P6L01_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDP3029233.1"
                     /translation="MEQTQKLKLNLQHFASNNVKPQVFNPDNVMMHEKKDGTLLNDFT
                     TPILQEVMENSKIMQLGKYEPMEGTEKKFTFWADKPGAYWVGEGQKIETSKATWVNAT
                     MRAFKLGVILPVTKEFLNYTYSQFFEEMKPMIAEAFYKKFDEAGILNQGNNPFGKSIA
                     QSIEKTNKVIKGDLTQDNIIDLEALLEDDELEANAFISKTQNRSLLRKIVDPETKERI
                     YDRNSDTLDGLPVVNLKSSNLKRGELITGDFDKLIYGIPQLIEYKIDETAQLSTVKNE
                     DGTPVNLFEQDMVALRATMHVALHIADDKAFAKLVPADAKPSSNPGEV"
     gene            7894..8181
                     /locus_tag="P6L01_000131"
     CDS             7894..8181
                     /locus_tag="P6L01_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029234.1"
                     /translation="MPEIIGIVKVDFTDLEDNRHVYMKGHVYPRKGYNPTDERIKALA
                     SVENKRNEQMIYIVNDKLTKKELVEIASVAGLQVDEKQTKAEIINAFESLE"
     gene            8190..8522
                     /locus_tag="P6L01_000132"
     CDS             8190..8522
                     /locus_tag="P6L01_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HDP3029235.1"
                     /translation="MTTLADVKKRIGLKDEKQDEQLEEIIKSCESQLLSMLPIEVEQI
                     PERFSYMIKEVAVKRYNRIGAEGMTSEAVDGRSNAYELNDFKEYEAIIDNYFNARTRT
                     KKGRAVFF"
     gene            8519..8821
                     /locus_tag="P6L01_000133"
     CDS             8519..8821
                     /locus_tag="P6L01_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029236.1"
                     /translation="MRYEDRVIFQLEQVATYNPKTSKKENTLITYDAIPCNINPISRA
                     RKQLEFGDVKNDVSVLRIKESISYPVSHVLVNGIRYKIVDTRIYRHETSYYIEEVN"
     gene            8821..9168
                     /locus_tag="P6L01_000134"
     CDS             8821..9168
                     /locus_tag="P6L01_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDP3029237.1"
                     /translation="MNIDGLDALLNQFHDMKTNIDDDVDDILQENAKEYVVRAKLKAR
                     EVMNKGYWTGNLSRNIRYKKTGDLQYTITSHAAYSGFLEFGTRYMEAEPFMWPVYEVI
                     RKSTVEELKALFE"
     gene            9180..9563
                     /locus_tag="P6L01_000135"
     CDS             9180..9563
                     /locus_tag="P6L01_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029238.1"
                     /translation="MTPNLQLYNKAYETLQGYGFPVISRKEMQQEIPYPFFVIKMPES
                     NRSKYTFDSYSGDTNLVIDIWSVSDDLGHHDGLVKRCIDDLTPSLKTNDYDFEEDDTN
                     ITQLVDDTTNQELLHTSVTISYKTF"
     gene            9582..10163
                     /locus_tag="P6L01_000136"
     CDS             9582..10163
                     /locus_tag="P6L01_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HDP3029239.1"
                     /translation="MANMKNSNDRIILFRKAGEKVDATKMLFLTEYGLSHEADTDTED
                     TMDGSYNTGGSVESTMSGTAKMFYGDDFADEIEDAVVDRVLYEAWEVESKIPGKNGDA
                     TKFKAKYFQGFHNKFELKAEANGIDEYEYEYGVNGRFQRGFATLPEAVTKKLKATGYR
                     FHDTTKEDALTSEDLTEIPQPKVDSSSVTPGEV"
     gene            10226..10591
                     /locus_tag="P6L01_000137"
     CDS             10226..10591
                     /locus_tag="P6L01_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HDP3029240.1"
                     /translation="MNVEINGKSLELSFGFKFLREIDNRLGLKVEQASIGQGVSMLPV
                     GLESGNPVVIGEVLIAATSHLKKQAITINNIDEALDEIAENIGLEEFGSDILTELGKR
                     PMTRNLVEVVEAEEKPAEA"
     gene            10621..10965
                     /locus_tag="P6L01_000138"
     CDS             10621..10965
                     /locus_tag="P6L01_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029241.1"
                     /translation="MSTLGITDLNVIEQMTLTEYNYRMYAKEYEMLTQEFERYKLAFA
                     IRDAAATKNVGTENKPKEEYVFNNANDVLPYEENIQRLNEGKDIRFSSERDEYEPQNN
                     EFFKVIAEFNKQ"
     gene            10982..14449
                     /locus_tag="P6L01_000139"
     CDS             10982..14449
                     /locus_tag="P6L01_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029242.1"
                     /translation="MTEYKIKATIEASVAKFKRQIDSAVKSVQRFKRVADQTKDVELN
                     ANDKKLQKTIKVAKKSLDAFSNKNVKAKLDASIQDLQQKILESNFELDKLNSKEASPE
                     VKLQKQKLTKDIAEAEVKLSELEKKRISIDVNADNSKFNRVLKVSKASLEALNRSKAK
                     AIIDVDNGVANSKIKRTKEELKSIPNKTRSRLDVDTGLSIPTIYAFKKSLDALPNKKT
                     TKVDVDTNGLKKAYAYIIKANDNFQRQMGNLANMFRVFGTVGSNMVGGLLTSSFSILI
                     PVIASVVPVVFALLNAIKVLTGGVLALGGAVAIAGAGFVAFGAMAISAIKMLSDGTLQ
                     ASSATNEYKKALDGVKSAWTDIIKQNQSAIFTTLANGLNTVKTAMQSLQPFFSGISRG
                     MEEASQSVFKWAQNSGVASRFFNMMNTTGVSVFNKLLSAAGGFGDGLVNVFTQLAPLF
                     QWSADWLDRLGQSFSNWANSAAGENSITRFIEYTKTNLPIIGNIFKNVFVGINNLMNA
                     FSGSSTGIFQSLEQMTANFREWSEQVGQSQGFKDFVSYIQTNGPLIMQLIGNIARGLV
                     AFATAMAPIASAVLRVAVAITGWIANLFEAHPATAQLVGVIITLVGAFRFLIPIILAV
                     SNFMGGGLIGGIIALVSKFGLLRAGLTILKGAFMLLKGPLKIISVIFQLLFGKIGLIR
                     NAITGLVTVFGILGGPITIVIGVIAALIAIFVLLWNKNEGFRNFIINAWNAIKTFMVT
                     VWNVLKTVASVVWNAILKAITTAVTNVYNFIMIVWNQIAAYLQGLWNGIIAIATTVWN
                     LLVTIITTVFTTIMTIVMTIWTAIWTFLSTIWNTIITIATTIWNLLVTVITTVFTTIM
                     TIAMTIWNAIWTFLQTLWNTIVTVATKVWNAITTAISTALQAAWSFISNIWNTIWSFL
                     SGILTTIWNKVVSIFTQVVSTISDKMSQAWNFIVTKGMQWVSTITSTLINFVNRVIQG
                     FVNVVNKVSQGMTNAVNKIKSFIGDFVSAGADMIRGLIRGIGQMAGQLVDAAKNVAKK
                     ALDAAKSALGIHSPSREFMDVGMYSMLGFVKGIDNHSSKVIRNVSNVADKVVDAFQPT
                     LNAPDISSITGNLSNLGGNINAQVQHTHSIETSPNMKTVKVEFDVNNDALTSIVNGRN
                     AKRNSEYYL"
     gene            14462..15409
                     /locus_tag="P6L01_000140"
     CDS             14462..15409
                     /locus_tag="P6L01_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDP3029243.1"
                     /translation="MDIELTKKDGTVIKLSEYGFIVNDIVIDSMQINTKYQDKENMNG
                     RILMGSNYISRDIVVPCFCKVKNRSDIAYMRDMLYSLTTDIEPMYLREIRRKEELNYR
                     FTQPTSDDYVKLDKNNFPDYEYSRHDQQIYVNGKQYKVIFNGVINPKQKGNKVSFELK
                     FETTELPYGESIGTSLELEENKKVGLWSFDFNIDWHAGGDKRKYTFENLSKGTVYYHG
                     SAPNDQFNMYKKITIILGEDTESFVWNLTHAEIMKIEGIKLKAGDRIVYDSFRVYKNG
                     VEISTETNIAQPKFKYGANKFEFNQTVQKVQFDLKFYYK"
     gene            15418..17316
                     /locus_tag="P6L01_000141"
     CDS             15418..17316
                     /locus_tag="P6L01_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDP3029244.1"
                     /translation="MTITIKPPKGNGAPVPVETTLVKKVNADGVLTFDILENKYTYEV
                     INAIGKRWIVSHVEGENDKKEYVITVIDRKSEGDRQLVECTAREIPIDKLMIDRIYVN
                     VTGSFTVERYFNIVFQGTGMLFEVEGKVKSSKFENGGEGDTRLEMFKKGLEHFGLEYK
                     ITYDKKKDRYKFVLTPFANQKASYFISDEVNANAIKLEEDASDFATFIRGYGNYSGEE
                     TFEHAGLVMEARSALAEIYGDIHAEPFKDGKVTDQETMDKELQSRLKKSLKQSLSLDF
                     LVLRESYPEADPQPGDIVQIKSTKLGLNDLVRIVQVKTIRGINNVIVKQDVTLGEFNR
                     EQRYMKKVNTAANYVSGLNDVNLSNPSKAAENLKSKVASIAKSTLDLMSRTDLIEDKQ
                     QKVSSKTVTTSDGTIVHDFIDKSNIKDVKTIGTIGDSVARGSHAKTNFTEMLGKKLKA
                     KTTNLAKGGATMATVTDTNNVENSIYRQAEQIRGDLIILQGTDDDWLHGYWAGVPIGT
                     DKTDTKTFYGAFCSAIEVIRKNNPTSKILVMTATRQCPMSGTTIRRKDTDKNKLGLTL
                     EDYVNAQILACSELDVPVYDAYHTDYFKPYNPAFRKSSMPDGLHPNERGHEVIMYELI
                     KNYYQFYG"
     gene            17331..19241
                     /locus_tag="P6L01_000142"
     CDS             17331..19241
                     /locus_tag="P6L01_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029245.1"
                     /translation="MDNKLITDLSRVFDYRYVDENEYNFKLISDMLTDFNFSLEYHRN
                     KEVFAHNGEQIKYEHLNVTSSVSDFLTYLNGRFSNMVLGHNGDGINEVKDARVDNTGY
                     GHKTLQDRLYHDYSTLDAFTKKVEKAVDEHYKEYRATEYRFEPKEQEPEFITDLSPYT
                     NAVMQSFWVDPRTKIIYMTQARPGNHYMLSRLKPNGQFIDRLLVKNGGHGTHNAYRYI
                     DGELWIYSAVLDSNKNNKFVRFQYRTGEITYGNEMQDVMPNIFNDRYTSAIYNPVENL
                     MIFRREYKPTERQLKNSLNFVEVRSADDIDKGIDKVLYQMDIPMEYTSDTQPMQGITY
                     DAGILYWYTGDSNTANPNYLQGFDIKTKELLFKRRIDIGGVNNNFKGDFQEAEGLDMY
                     YDLETGRKALLIGVTIGPGNNRHHSIYSIGQRGVNQFLKNIAPQVSMTDSGGRVKPLP
                     IQNPAYLSDITEVGHYYIYTQDTQNALDFPLPKAFRDAGWFFDVLPGHYNGALRQVLT
                     RNSTGRNMLKFERVIDIFNKKNNGAWNFCPQNAGYWEHIPKNITKLSDLKIVGLDFYI
                     TTEESKRFTDFPKDFKGIAGWILEVKSNTPGNTTQVLRRNNFPSAHQFLVRNFGTGGV
                     GKWSLFEGKVVE"
     gene            19241..21064
                     /locus_tag="P6L01_000143"
     CDS             19241..21064
                     /locus_tag="P6L01_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDP3029246.1"
                     /translation="MIVDNFSKDDNLIELQTTSQYNPIIDTNISFYESDRGTGVLNFA
                     VTKNNRPLSISSEHVKTSIVLKTDDYNVDRGAYISDELTIVDAINGRLQYVIPNEFLK
                     HSGKVHAQAFFTQNGSNNVVVERQFSFNIENDLVSGFDGITKLVYIKSIQDTIEAVGK
                     DFNQLKQNMADTQTLIAKVNDSATKGIQQIEIKQNEAIQAITATQTSATQAVTAEVDK
                     IVEKEQAIFERVNEVEQQINGADLVKGNSTTNWQKSKLTDDYGKAIESYEQSIDSVLS
                     AVNTSRIIHITNATDAPEKTDIGTLEKPGQDGVDDGSSFDESTYTSSKSGVLVVYVVD
                     NNTARATWYPDDSNDEYTKYKIYGTWYPFYKKNDGNLTKQFVEETSNNALNQAKQYVD
                     DKFGTTSWQQHKMTEANGQSIQVNLNNAQGDLGYLTAGNYYATRVPDLPGSVESYEGY
                     LSVFVKDDTNKLFNFTPYNSKKIYTRSITNGRLEQQWTVPNEHKSTVLFDGGANGVGT
                     TINLTEPYTNYSILLVSGTYPGGVIEGFGLTALPNAIQLSKANVVDSDGNGGGIYECL
                     LSKTSSTTLRIDNDVYFDLGKTSGSGANANKVTITKIMGWK"
     gene            21064..21441
                     /locus_tag="P6L01_000144"
     CDS             21064..21441
                     /locus_tag="P6L01_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HDP3029247.1"
                     /translation="MKITVNDKNEVIGFVNTGGLRNSLDVDDNNVPIKFKEEFEPRKF
                     VFTNGEIKYNSNFEKEDVPNASNQQSASDLSDEELRGMVASMQMQVAQVNVLTMELAQ
                     QNAMLTQQLTELKTNKTSTEGDV"
     gene            21445..21618
                     /locus_tag="P6L01_000145"
     CDS             21445..21618
                     /locus_tag="P6L01_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDP3029248.1"
                     /translation="MKMIYPTFKDIKTFYVWGYYKNEQIKWYVDKGLIDKEEYALITG
                     EKYPETKDEKSQV"
     gene            21658..21957
                     /locus_tag="P6L01_000146"
     CDS             21658..21957
                     /locus_tag="P6L01_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HDP3029249.1"
                     /translation="MFGFTKRHEQDWRLTRLEENDKTMFEKFDRIEDSLRAQEKIYDK
                     LDRNFEELKRDKVEDEKNKEKNAKNIRDIKMWILGLIGTIFSTIVIALLRTIFGI"
     gene            22094..23992
                     /locus_tag="P6L01_000147"
     CDS             22094..23992
                     /locus_tag="P6L01_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDP3029250.1"
                     /translation="MGLPNPKNRKPTASEVVEWALYIAKNKIAIDVPGSGMGAQCWDL
                     PNYLLDKYWGFRTWGNADAMAQKSNYRGRDFKIIRNTKDFVPQPGDWGVWTGGWAGHV
                     NIVVGPCTKDYWYGVDQNWYTNNATGSPPYKIKHSYHDGPGGGVKYFVRPPYHPEKST
                     PAPKPEDDSDNNEKNNKKVPIWKDVTTIKYTISSQEVNYPEYIYHFIVEGNRRLEKPK
                     GIMIRNAQTMSSVENLYNSRKKYKQDVEYPHFYVDRHNIWAPRRAVFEVPNEPDYIVI
                     DVCEDYSASKNEFIFNEIHAMVVAVDMMIKYEIPLSIENLKVDDSIWRSMLEHVNWNM
                     IDNGAPPKDKYEALEKALFNIFKNREKLLNSITKPTVTKSRIKVMVDNKNADIANVRD
                     SSPTANNGSASKQPQIITETSPYTFKQALDKQMARGNPKKSNAWGWANATRAQTGSAM
                     NVKRIWESNTQCYQMLNLGKYQGVSVSSLNKILKGKGTLNNQGKAFAEACKKHNINEI
                     YLIAHAFLESGYGTSNFANGKDGVYNYFGIGAYDNNPNYAMTFARNKGWTTPAKAIMG
                     GASFVRKDYINKGQNTLYRIRWNPKNPATHQYATAIEWCQHQASTIAKLYKKIGLKGI
                     YFIRDKYK"
     gene            24005..25243
                     /locus_tag="P6L01_000148"
     CDS             24005..25243
                     /locus_tag="P6L01_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HDP3029251.1"
                     /translation="MYKIKDVETRIKNDGVDLGDIGCRFYTEDENTASIRIGINDKQG
                     RIDLKAHGLTPRLHLFMEDGSIFKNEPLIIDDVVKGFITYKIPKKVIKHAGYVRCKLF
                     LEKEEEKIHVANFSFNIVDSGIESAVAKEIDVKLVDDAITRILKDNATDLLNKDFKEK
                     IDKDVISYIEKNESRFKGAKGDKGEPGQPGAKGEAGKKGEQGAPGKNGTVVSINPDTK
                     MWQIDGKDTDIKAEPELLDKINIANVEGLEDKLQEVEKIKDTTLNDSKTYTDSKIAEL
                     VDSAPESMNTLRELAEAIQNNSISESVLQQIGSKVSTEDFERFKQSLNSLYADKNHSH
                     TIKQIEGLENALSKKSDINHSHDERYLLSSNAFTKEEADKLYQPIGSSQPSLNIWTGS
                     ETEYNYLYQKDPNTLYLIKG"
     gene            25249..25644
                     /locus_tag="P6L01_000149"
     CDS             25249..25644
                     /locus_tag="P6L01_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029252.1"
                     /translation="MEGNFKNVKKFIYEGEEYTKVYAGNIQVWKKPSSFVIKPLPKNK
                     YPDSIEESTAKWTINGVEPNKSYQVTIENVRSGIMRISQTNLRSSDLGISGVNSGVAS
                     KNINFSNPSGILYVTISDVYSGSPTLTIE"
     gene            25700..25975
                     /locus_tag="P6L01_000150"
     CDS             25700..25975
                     /locus_tag="P6L01_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDP3029253.1"
                     /translation="MDIGTIVRTILLIVAWINQFLAIKHISPIPVDEVFISTVVTGIV
                     SIWTWWKNNNFTHASKKGQQKIYEVKAGIQSTGGAPKVNGDDNNAVG"
     gene            25962..27374
                     /locus_tag="P6L01_000151"
     CDS             25962..27374
                     /locus_tag="P6L01_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDP3029254.1"
                     /translation="MPSVRTYSQAISYLKSLEGKAWNPDNAFGCQCFDTANQYWLYLF
                     NHRLKGVGAADIPTWNDFTNEATVYENTVSFQALPGDVVIFNRNYGGGYGHVGIVISA
                     TLDSITILEQNWLGGAYWSPPEVTTRRTHGYDFPMWFIRPFYAKETTANKLRSAVKPV
                     KQDKLSKGKKIMLVAGHGIGAYSNDPGAVANGENERDFNRKNIIPRVKKYLESVGNTV
                     LLYGGNSMNQDLYQDTLYGQRVGNYKDYGMYWIKSEVKPDAIIEFHLDSASPQASGGH
                     VIISDRFPADDIDKALSSALDKTVGKIRGVTPRGDLLNANVSADLNLNYRLIELGFIT
                     STKDLNYIKNNLDSFTKRIAEAINGRQIDAPSSKPSADKITWNWKGVFYPNPEKAIRV
                     RKTAGLTGTVVEEDSWLYTKDDWVKFDQVIKKDGYWWIRFKYQREGSSTNNFYCAVCR
                     ITDKEQKIKNEKYWGTIEWA"
     gene            complement(27434..27991)
                     /locus_tag="P6L01_000152"
     CDS             complement(27434..27991)
                     /locus_tag="P6L01_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HDP3029255.1"
                     /translation="MNKKLLTKTLIASALVLTTVGSGFHSSSNYNGINNVAKASEITD
                     SELWKNVRDALKDANIIDKTDKETIKVKYKLKNGGESEISGTANLDNLSNTNNSTVSP
                     DSVNRVDITRVNPNGNTIEANDAWKKLVDKLKEKHIVKVGDKVTIHSKDPSDEKVYGK
                     VGDQDSNVKNRLISPKDITHITIER"
     gene            28366..28518
                     /locus_tag="P6L01_000153"
     CDS             28366..28518
                     /locus_tag="P6L01_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029256.1"
                     /translation="MCKEVKSLMLDIIKTLLEHQVLAVLIIPEVLKQLREWHLGYLDR
                     KPNNKD"
     gene            28589..28699
                     /locus_tag="P6L01_000154"
     CDS             28589..28699
                     /locus_tag="P6L01_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029257.1"
                     /translation="MTEQMYLILFLLSLPLLLFIGRKTHFYCLDKKNGRR"
     gene            28701..28886
                     /locus_tag="P6L01_000155"
     CDS             28701..28886
                     /locus_tag="P6L01_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029258.1"
                     /translation="MSDYKLKIIELIKSDITGYQIHKQTGVAQYVISQLRQGKREVDN
                     LTLNTTEKLYSYARQVL"
     gene            29571..29710
                     /locus_tag="P6L01_000156"
                     /pseudo
     CDS             29571..29710
                     /locus_tag="P6L01_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499406.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(29716..30030)
                     /locus_tag="P6L01_000157"
     CDS             complement(29716..30030)
                     /locus_tag="P6L01_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029259.1"
                     /translation="MYFVLAIFTIISASVSLGYSIQACASSHNINAYYALSRSLPLFL
                     LAIFSLVIHSAIFLITISIAMILVQFLDAIVGYKSEDVFKTYGPLATSVVNLILLIVF
                     LF"
     gene            30395..31435
                     /locus_tag="P6L01_000158"
     CDS             30395..31435
                     /locus_tag="P6L01_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDP3029260.1"
                     /translation="MIIAIIILIFISFFFSGSETALTAANKTKFKTEADKGDKKAKGI
                     VKLLEKPSEFITTILIGNNVANILLPTLVTIMALRWGISVGIASAVLTVVIILISEVI
                     PKSVAATFPDKITRLVYPIINICVIVFRPITLLLNKLTDSINRSLSKGQPQEHQFSKE
                     EFKTMLAIAGHEGALNEIETSRLEGVINFENLKVKDVDTTPRINVTAFASNATYEEVY
                     ETVMNKPYTRYPVYEGDIDNIIGVFHSKYLLAWSNKKEDQITNYSAKPLFVNEHNKAE
                     WVLRKMTISRKHLAIVLDEFGGTEAIVSHEDLIEELLGMEIEDEMDKKEKEKLSQQQI
                     QFQQRKNRNVSI"
     gene            31449..32516
                     /locus_tag="P6L01_000159"
     CDS             31449..32516
                     /locus_tag="P6L01_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDP3029261.1"
                     /translation="MWNKNRLTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIG
                     AGYFNTQQLEDEIDYVRQLTSNSFGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLE
                     EPVVKIIEEQQFKCHIDTIIKKQVPVCCFTFGIPNESIIERLKEANIKLIGTATSVDE
                     AIANEKAGMDAIVAQGSEAGGHRGSFLKPKNQLPMVGTISLVPQIVDVVSIPVIAAGG
                     IMDGRGVLASIVLGAEGVQMGTAFLTSQDSNASELLRDAIINSKETDTVVTKAFSGKL
                     ARGINNRFIEEMSQYEGDIPDYPIQNELTSSIRKAAANIGDKELTHMWSGQSPRLATT
                     HPANTIMSNIINQINQIMQYK"
     gene            32770..32853
                     /locus_tag="P6L01_000160"
     CDS             32770..32853
                     /locus_tag="P6L01_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029262.1"
                     /translation="MIIIKRKHQAEDRRKILIKRKHQIKVR"
     gene            32901..33749
                     /locus_tag="P6L01_000161"
     CDS             32901..33749
                     /locus_tag="P6L01_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDP3029263.1"
                     /translation="MINIGLTGWGDHYSLYEDLERQTDKLKTYAGHFPVVELDATYYA
                     IQPERNILKWIKETPDTFEFVVKIHQALTLHADYKTFADTRQELFDQFKNMLEPLHTQ
                     KKLAMVLVQFPPWFDCNAQNIKYILYVRQQLQAFPMCVEFRHQSWFSDAFKEQTLAFL
                     TEHQIIHAVVDEPQVKDGSVPLVNRITNEIAFVRYHGRNHYGWTKKDMSDQEWRDVRY
                     LYDYNEQELIDLAQKAQILAQKAKKVYVIFNNNSGGHAANNAKTYQRLLNIEYEGLAP
                     QQLKLF"
     gene            33762..34589
                     /locus_tag="P6L01_000162"
     CDS             33762..34589
                     /locus_tag="P6L01_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDP3029264.1"
                     /translation="MLLTITLLVLIGGLSAIIGSIVGIGGGIIIVPTMVYLGVEHGLL
                     HNITTQVAIGTSSVILIVTGLSSSLGYLKTKQVDIKNGSIFLFGLLPGSLLGSFISRY
                     LTFESFNLYFGIFLIFVAILLMVRNKIKPFKIFDKPKYEKTYVDAKGKTYHYSVPPLF
                     AFITTFLIGILTGLFGIGGGALMTPLMLIVFRFPPHVAVGTSMMMIFFSSVMSSIGHI
                     AQGHVAWGYAIILIISSYFGAKIGVKVNQSIKSDTVVTLLRTVMLLMGIYLIIRALI"
     gene            34616..35935
                     /locus_tag="P6L01_000163"
     CDS             34616..35935
                     /locus_tag="P6L01_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDP3029265.1"
                     /translation="MRLTIYHTNDIHSHLHEYERIKVYMAEQRPRLNHPSLYVDLGDH
                     VDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSN
                     VIDESGHLPNNIVSSYIKDMDGVKILFVAATAPFTPFYRALDWIVTDPLESIKEEIKH
                     QRGKFDVIIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKY
                     GNYLGEVNLTFEAHKVVHKTAKIIPLETLPEVETSFEEEGKTLMSNSVIQHPVVLKRS
                     MNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRV
                     RLSGVKLKEIITKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLNGEEI
                     EDDKEYVLGTIDMYTFGRYFPTLKELPKEYLMPEFLRDIFKEKLLEY"
     gene            36019..36936
                     /gene="lipA"
                     /locus_tag="P6L01_000164"
     CDS             36019..36936
                     /gene="lipA"
                     /locus_tag="P6L01_000164"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDP3029266.1"
                     /translation="MATKNEEILRKPDWLKIKLNTNENYTGLKKMMREKNLNTVCEEA
                     KCPNIHECWGARRTATFMILGAVCTRACRFCAVKTGLPNELDLNEPERVAESVELMNL
                     KHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASR
                     PDILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEI
                     YETMDDLRANDVDILTIGQYLQPSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGP
                     LVRSSYHADEQVNEAAKEKQRQGEAQLNS"
     gene            37066..37449
                     /locus_tag="P6L01_000165"
     CDS             37066..37449
                     /locus_tag="P6L01_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDP3029267.1"
                     /translation="MIKVDQHYFELIENYRECFNEEQFIARYSDILDKYDYIVGDYGY
                     DQLRLKGFYKDSNKKAEMSKRFSNIQDYIFEYCNFGCPYFVLRHLSKQEVKKLIEEVH
                     PSDVIDDDNKLQDVKIKPTIQDTEH"
BASE COUNT        13871 a         5414 c         7369 g        10878 t
ORIGIN      
        1 acaatactta gtcacaacat tcaaagattc aacaggacaa ccacatgaac attttactac
       61 tgctagagat aatcagacgt ttacagttgt tgaggcagag agtaaagaag aagcgaaaga
      121 aaagtacgag gcacaagtta aaagagatgc aattattaaa ttaggtcagt tgtttgaaaa
      181 tataagggag tgtcggaaat gacggatgtt aaaattaaaa ctatttcagg tggagtttat
      241 tttgtaaaaa cagctgaacc ttttgaaaaa tatgttgaaa gaatgacgag ttttaatggt
      301 tatatttacg caagtactat aatcaagcaa ccaacgtata ttaaaacaga tacgattgaa
      361 tcaatcacac ttattgagga gcgtgggaaa tgaatcagct gagaatttta ttacatgacg
      421 gtagtagttt gatattacat gaagatgaat tatttaacga aatagtattt gttttggatg
      481 attttagaaa tgatgatgac tatttaacga tagaaaaaga ttatggcaga gaacttgtat
      541 tgaacaaagg ttatatagtt gggattaatg ttgaggaggc agacgatgac taaacaaata
      601 ttaagactat tattcttact agcgatgtat gagctaggta agtatgtaac ggagcaagta
      661 tatattatga tgacggctaa tgatgatgta gagatgccga gtgacttcgc aaagttgagc
      721 gatcagtctg atttgatgag ggcggaggtg tcagagtaga tgatgtggtt agtcatagca
      781 attatattac tagtcatctt attgtttggt gtgatgttgc aagctgaaca gttaaaaggc
      841 gatgtgaaag ttaaagagcg gtagatagag atattaagaa gtagattgag acattttgaa
      901 gattaaacat atttgtacgg agggtattca tgactaaaaa gaaatacgga ttaaaattat
      961 caacagttcg gaaattagaa gacgagttgt gcgattatcc taattatcat aaacaacttg
     1021 aagatttaag aagtgaaata atgacaccgt ggattccaac agatacaaat ataggcgggg
     1081 agtttgtacc atctaataca tcaaaaacag aaatggcagt aactaattat ctttgtagta
     1141 tacgaagagg taaaattctt gagtttaaga gtgcgattga acgtataatc aacacatcaa
     1201 gtaggaaaga acgcgaattc attcaagagt attattttaa taaaaagact ttgattgcgg
     1261 tttgttatga catacacatc tctgaaagta cagcgcatag aatcaagaag aaaatagtgt
     1321 ctaaactagc cgaagaatta ggagaatact aaatttgaca gtaaaatgac agtttttgac
     1381 acctataacg agatattatg atagtgtagg atattgacta tcttactgcg tttcccttat
     1441 cgcaattagg aataaaggat ctatgtgggt tggctgatta tagccaatcc cttttttaat
     1501 tttaaaaagc gtatagcgcg agagttggtg gtaaatgaaa tgaacaaatt aactaaaaag
     1561 caacgtttgt ttgcagaagt atatacaata cctggtactg aatgttatgg caatgctact
     1621 aagtcagctg tgcatgccgg atatagcgaa aagacggcgt actcacaagg acagcgtatg
     1681 ttgaagaatg ttgaaattca gaattatatc aaggaggttg aaacaaaact ctttgacgag
     1741 aatattatgt caggtaaaga agtgttgtat aggctaacta gaacagctag aggagaacac
     1801 acggaagttg aagctgtcgt aacaaaaact ggagactata aagagaatcc ggatactggc
     1861 aaaatgcaat tagtatacga tgaacacata caacttgtta ctaagtcacc taaaataagt
     1921 gaccaaaaca aagccttaga gatgttaggt agacatcaca aattatttac agacaaacaa
     1981 gaagtcgacc acaaaatacc gatgtttgtt gataatattc cggaagatga ttagtcatgt
     2041 atgaaatact tgatctaaaa aataaaatcg gtggtggcta caataagttt tggcacaaca
     2101 aaaactttta ccgtgttgtt aaaggttcaa ggggtagcaa gaaaagtaaa actaccgcta
     2161 ttaatctcat ttatcgaata atgaaatatg attgggcaaa tatacttgta gtcagaagat
     2221 ttagcaacac taacaaacaa tcaacgtata cagatttaaa gtgggcaact aaccaattag
     2281 gcgttgctca cttatttaaa ttcaacgaaa gtttgccgga aataacgtat aaacctactg
     2341 gacaaaaaat actgtttaga ggtttagacg acccattgaa aataacatcg attactgttg
     2401 atacaggcat tttgtgttgg gcttggtttg aagaggctta tcaaatagaa acattcgcta
     2461 agtttagcac tgttgttgag tcaatacgtg gtagctacga tagtccggaa tttttcaagc
     2521 aaatcacagt cacttttaac ccgtggtcgg aaagacattg gttgaagcct acattttttg
     2581 atgaagaaac aaaattaaac aatacttttt cagatacaac aacttataga gttaatgaat
     2641 ggctagataa agtcgatatt gaacgatatg aagatttgta tataaagaat cctagacgtg
     2701 caagaatcgt ttgtgatgga gattggggtg ttgcagaggg gcttgtattc gataatttta
     2761 aagtggaaga ctttgattgg tttgaggagt ttaaaagaac gcaagaaata actcacggaa
     2821 tggattttgg atttagtcaa gaccctacaa cagttgttag tacggttgta gatttaaaaa
     2881 acaaaaagtt attcatctat gatgaacact ataaaaaagc gatgttaact gatgatataa
     2941 aacaaatgct tattaaaaaa ggattaggtg atgtagatat tgcagctgat tatggggctg
     3001 gtggagatag agtgatcagt gaattgaaat ctaaagggat taaaggtata agaaaagcgt
     3061 tgaaaggcgc taatactatt ttaccaggca ttcaattcat tcaaggcttt gaagttatta
     3121 tacacccatc atgtgaacac gctattgaag agttcaacac ttatacattt gaccaagata
     3181 atgatggtaa gtggttgaac aagcctatag atgctaataa ccatattatc gatgcattgc
     3241 gttatagtct tgagaaatat catatcgtac gtaaaaaacg taaaaagaat atagaaagca
     3301 aaacaaaagt aattaaatct ctaggattat aggagggaac aaatgttaaa ggcaaacgaa
     3361 tttgaaacgg atactgattt acgagaaaac agaaattact tgtttaacga tgaagctaat
     3421 gttgtttaca catatgacgg gacagagtct gatttattac aaaacattaa tgaagtaagt
     3481 aaatacattg aacatcacat ggattaccaa cgacctagat taaaagtgtt aagtgattat
     3541 tacgaaggta aaactaagaa tctggttgag ttaacacgac gcaaagaaga gtacatggca
     3601 gataaccgtg tagcgcatga ttacgcatct tatattagcg attttattaa cggttatttc
     3661 ttaggtaatc cgattcaatg tcaagatgat gataaagatg tattagaagc tattgaggcg
     3721 ttcaatgatt taaatgatgt tgagtcacac aatagatctt taggattaga tttgtcaatt
     3781 tatggcaaag cttatgagtt aatgattaga aaccaagatg atgaaacgcg tttatacaag
     3841 agtgatgcaa tgagtacttt tgtcatatac gacaatacaa ttgaacgtaa tagtatcgca
     3901 ggagttagat atttaagaac taaaccaata gacaagactg acgaagatga agtgtttaca
     3961 gttgatttat ttacttctca cggtgtttat agatatctta ccagtagaac aaatggattg
     4021 aagctcacac cacgtgaaaa cggttttgaa tcacactctt tcgaacgtat gcctattaca
     4081 gaatttagca acaacgaaag aagaaaaggg gattatgaga aagtaatcac tttaattgat
     4141 ttgtatgata atgctgaatc agatactgct aactatatga gtgatttaaa tgacgctatg
     4201 ttacttatta aaggtaattt aaatttagat cctgtagaag ttagaaaaca aaaggaagct
     4261 aacgtgttat ttttagagcc aaccgtttat gagaataggg atacaggtat cgaaacagaa
     4321 ggttcagttg acggcggtta tatttataaa caatacgatg tacaaggtac cgaagcttat
     4381 aaagaccgtt tgaacagtga tatacacatg tttaccaaca cgcctaacat gaaagatgat
     4441 aactttagtg gcactcaatc gggcgaggca atgaaataca aattattcgg attagaacaa
     4501 cgtactaaaa ctaaagaagg attgttcact aaagggttaa gacgtcgtgc taagttgtta
     4561 gagacaatac ttaaaaatac acggtcgatt gacgctaaca aagatttcaa tactgttaga
     4621 tacgtataca acagaaactt acctaaatca ttaatcgaag aattaaaagc ttatattgat
     4681 tctggcggga agattagtca aacaacttta atgtctctat tctcgttctt ccaagaccct
     4741 gaattggaag tcaagaaaat agaagaagat gagaaagaat ctattaaaaa agctcaaaaa
     4801 ggtatttata aagaccctag agacatcaat gatgacgaac aagatgatga tacaaaagat
     4861 actgttgata aaaaggaatg attgtaattg cctaacaaaa acactcaaga atattgggaa
     4921 gaacgcggac gcaaagcaat cgagaatgag ttgaagcgtg ataaaactaa agctgaagaa
     4981 atagaacgta tattgaatat gatgattaag cgcattgaaa aagaaatcaa tgcgtttatt
     5041 gttaagtacg gagattttgc aggcgttaca ttacaagaag cacaaaagat tattgatgag
     5101 ttcgatgtaa aagcgtttca agaagaagca aaaagattgg tcgaaaacaa ggactttagc
     5161 gatagagcaa atgaagaatt aaagaagtat aacactaaga tgtatgtatc tagagaacag
     5221 atgttaaaga ttcaaataga attcttaatt gcttatgcaa cagctcaaac agaattatcg
     5281 atgagggaat atttcgaatc aacagcttat cgtgtgttca gtgatcaagc gggtatttta
     5341 ggtgaaggtg tacaagtagc taaagaagtt atagatacaa tcgttgatac acaatttcat
     5401 ggtgtcgttt ggtcagagcg attatggact aataccgaag caatgaaaca agaagtagaa
     5461 gaaataattg ctaatgtagt tattagaggt cgacatccaa atgaatacgt taaagatatg
     5521 cgcaagcact taaataaatt cgaaggaaca gcacgacaaa agaccgcagc aattaaatca
     5581 ttgctttaca cggaatcggc acgtgttcac gcacaatcaa gtattgacag tatgaaagaa
     5641 atctctccag aaggatatta tatgtatatt gcaaaaatcg ataatagaac aactaaagta
     5701 tgtaaagggc ttaatggaga agtattcaaa gttaaagacg ctaaaattgg tgttaatttc
     5761 taccctatgc atatcaattg tcgttcagat tgcgctttat tacctaaatc catgtggccg
     5821 aaaaaacaga acaagaaacg aaaaacaaaa tactttggag ggaaagtgaa aagcggtgat
     5881 tgatttgaaa gtgaaggttt ttagaggcaa aaaaggtaag ttagctttgt atgacagtga
     5941 attaaaaaat tggaggatac taatatgagt aatactgaca aataccttag agacatagca
     6001 agagagttga aatgtatacg taaagagtta caaaagcgga atgagatgat tgaagataac
     6061 aatcaaaaat tagaagaaag tttgggaaaa ctttttactg aattaacagg tagtaatatg
     6121 gaaattaaac ttcagtagct agcacttaat tgtgttggct attttttatg tccaaaccat
     6181 gcttatgaca ataaaaggtg caagcgttac agcccgaacc atgtatggct taaaactaat
     6241 caagagtaaa taaatgaggt gtaaaaacta tggatatcca agagaagtta aaactcaaat
     6301 tacagttttt tgctgaagaa tcagatggag ataatggaaa atcaaaagat aacaacgatg
     6361 atgaaggcaa agacaaacaa gacaaaaaga ctaattcaga agaagaaatt gaaaaaagac
     6421 tacaagaaga atataacaag cgtcttaaag aagaattaag tcgtcgtatg aagcagaaag
     6481 aaaaagagaa acaagaagct gttgatgaag ctaaacgatt agcaaaaatg aacaaagatc
     6541 aaatcgctga atatgaacgc gaacaaatgg aaaaagagtt ggaacaacta cgttcagaaa
     6601 aacaattaaa tgaaatgcgt tcagaagcac gaaaaatgtt aagtgaagca gaagttgatt
     6661 catcagatga ggttgttaat ttagttgtaa cagatactgc tgaacaaact aaattgaatg
     6721 ttgaagcttt ttctaatgca gtaaaaaaag cggttaatga agcggttaag gttaacgcta
     6781 gacaatcgcc attgactggt ggagattcat ttaatcactc gactaaaaat aaaccgcaaa
     6841 acttagctga aatagctaga caaaaaagaa ttattaaaaa ttaacggagg catttaaatg
     6901 gaacaaacac aaaaattaaa attaaatttg caacattttg caagtaacaa tgttaaacca
     6961 caagtattta accctgacaa tgtaatgatg cacgaaaaga aagatggcac gttgttaaac
     7021 gactttacaa cacctatctt acaagaggtt atggaaaatt ctaaaatcat gcaattaggt
     7081 aagtacgaac caatggaagg tactgagaag aagtttactt tttgggctga taaaccaggt
     7141 gcttactggg taggtgaagg tcaaaaaatt gaaacgtcta aggctacttg ggttaatgct
     7201 acaatgagag cgtttaaatt aggggttatc ttacctgtaa caaaagaatt cttgaattac
     7261 acttattcac aattctttga agagatgaaa cctatgattg ctgaagcttt ctataaaaag
     7321 tttgatgaag cgggtatttt gaatcaaggt aacaatccat tcggtaaatc aattgcgcaa
     7381 tcaattgaaa aaactaataa ggttattaaa ggtgacctca cacaagataa cattattgat
     7441 ttagaggcat tacttgaaga tgacgaatta gaagcaaatg cgtttatctc aaaaacacaa
     7501 aacagaagct tgttacgtaa aattgtagat cctgaaacga aagaacgtat ttatgaccgt
     7561 aacagtgata cgttagatgg tctacctgtg gttaacctta aatcaagcaa cttaaaacgt
     7621 ggtgagttaa tcactggtga tttcgataag ttgatttacg gtatccctca attaattgaa
     7681 tacaaaatcg atgaaactgc acaattatct acagtcaaaa atgaagatgg aacacctgta
     7741 aacttgtttg aacaagacat ggtggcatta cgtgcaacta tgcatgtagc attgcatata
     7801 gctgatgata aagcgtttgc taagttagtt cctgctgatg caaaaccatc ttcaaatcca
     7861 ggagaagttt aataaataat taggagtggt aacatgcccg aaatcattgg aattgttaaa
     7921 gtagatttta cagatttaga agataacaga catgtctata tgaaagggca tgtctaccct
     7981 cgaaaaggtt ataatcctac agatgaacgt atcaaagctt tagctagtgt tgaaaataaa
     8041 cgcaacgaac aaatgattta cattgtaaat gacaaattaa ccaaaaaaga acttgtcgaa
     8101 atagcaagtg ttgctggctt acaagttgat gaaaaacaaa caaaagctga aattatcaat
     8161 gcttttgagt cactagagta ggtggttata tgactacgct agctgatgta aaaaaacgta
     8221 ttggtcttaa agatgaaaag caagatgaac aattagagga aattataaaa agttgtgaaa
     8281 gccagttgtt atcaatgtta cctattgaag ttgaacaaat accggaaagg tttagttaca
     8341 tgattaaaga agttgcagtt aaacgctaca acaggattgg tgctgaaggt atgacatcag
     8401 aagcggttga cggacgtagc aatgcgtatg aattgaacga tttcaaggag tatgaagcta
     8461 ttattgataa ttactttaat gctagaacga gaactaaaaa aggaagggct gtgttctttt
     8521 gagatatgaa gatagagtta tttttcaatt agaacaagta gcaacttaca atcctaaaac
     8581 tagcaaaaaa gaaaacacac taatcactta tgatgcgata ccatgcaata ttaaccccat
     8641 ttctagagca agaaagcaac ttgaatttgg tgacgtaaaa aacgatgtaa gtgttctgag
     8701 gataaaagaa tcaatatctt accctgttag ccacgtgttg gttaatggca ttcgctacaa
     8761 gatagttgat acaaggatat acagacacga aacgtcatat tatatcgaag aggtcaattg
     8821 atgaatatag atggattaga cgcactgtta aaccaatttc acgatatgaa aaccaacatt
     8881 gatgatgatg tagatgatat tttacaggaa aacgccaaag aatatgtagt acgagctaaa
     8941 ttgaaagcta gagaagtaat gaataagggt tattggactg gtaatttatc acgcaatatc
     9001 agatataaaa aaactggcga tttgcaatac actatcacat cgcatgcagc ttatagtggt
     9061 ttcttagagt ttggtactcg atacatggag gcggaacctt ttatgtggcc agtatatgag
     9121 gtaataagaa aatcaactgt agaagaattg aaagcgttgt ttgaatagga gataaaagca
     9181 tgacaccgaa cttacaactt tataataaag cgtatgaaac gctacaagga tatggattcc
     9241 ctgttatttc tcgtaaagag atgcaacaag agattccgta tccttttttt gtaataaaaa
     9301 tgccggagtc aaacagaagt aaatacacgt ttgatagtta ttctggtgac acgaatttag
     9361 ttattgatat ttggagtgta agtgatgatt taggacatca tgacggactt gttaaaagat
     9421 gtattgatga tttaacacct agccttaaaa caaacgatta tgactttgaa gaagatgata
     9481 ctaacatcac acagttagtt gatgatacta ccaatcaaga attgctacac acatcagtaa
     9541 cgatatctta caaaacattt taaaaaacgg aggaatattg aatggcaaat atgaaaaata
     9601 gtaatgatcg tattatttta tttagaaaag ctggcgaaaa agtagatgct actaaaatgc
     9661 tttttttaac tgaatacggc ttatcacatg aagctgatac agatacagag gatacaatgg
     9721 acggttctta taacactggt ggttctgttg agtcaacaat gtctggtact gctaaaatgt
     9781 tttatggtga cgattttgca gatgaaattg aagatgcagt tgtagatcgc gtattgtatg
     9841 aagcttggga agttgaaagt aaaataccag gcaaaaatgg ggatgctact aaatttaaag
     9901 cgaaatattt ccaaggtttc cacaataaat ttgaattaaa agcagaagct aacggtattg
     9961 atgaatatga atatgaatat ggagtgaatg gtcgtttcca acgtggattt gcaacactac
    10021 ctgaggctgt aacaaagaaa cttaaggcga ctggatacag attccacgac actacaaaag
    10081 aagatgcgtt aactagtgaa gatttaaccg aaattccaca acctaaagta gattcatcat
    10141 cggttacacc aggagaggta taaaaatagg gcttaacgcc ctttttattt ttgtttaaat
    10201 taatcatgaa tggagatttt aaattatgaa tgtagaaatt aatggaaagt cattagagtt
    10261 aagttttggt tttaaatttt taagagaaat tgataaccga ttaggtttaa aagttgaaca
    10321 agcttctatc ggtcaaggtg tatcaatgtt gcctgtaggt ttagaaagtg gaaatccggt
    10381 tgtgattggc gaagttttaa tcgcagctac atctcactta aaaaaacaag caattactat
    10441 taataacatt gatgaagcat tagatgaaat cgcagaaaat atcggactag aagaattcgg
    10501 ttcggatatt ttaacggagt tgggaaagcg acctatgacc cgaaacctag tcgaagtagt
    10561 ggaagcggaa gagaaaccag cggaagcgta ataacttacg acagaatcgt tataacttgt
    10621 atgtcaacac ttggtattac agatttgaac gttattgagc aaatgacatt aacagaatat
    10681 aactatcgaa tgtatgcgaa agagtatgaa atgctaaccc aagaattcga acgttacaaa
    10741 cttgcgtttg ctattcgtga tgctgcagct actaaaaatg ttgggacaga aaataaacct
    10801 aaagaggaat atgtttttaa caatgcaaac gacgtattgc cttatgaaga aaatatccaa
    10861 cggcttaacg aaggtaaaga tataagattt agcagcgaac gtgatgaata cgaaccacaa
    10921 aataatgaat tctttaaagt tatagcagaa tttaataagc aatagaaaga gaggtgttaa
    10981 tgtgacggaa tataaaatta aagcgactat tgaagctagt gtagccaaat tcaaaaggca
    11041 aattgatagt gcggttaagt ctgtgcaaag atttaaacga gtagcagatc aaactaaaga
    11101 tgttgaatta aacgctaacg ataaaaaatt acaaaaaact atcaaggttg ctaaaaagtc
    11161 tttagatgcc tttagcaaca aaaatgtaaa agctaaatta gatgctagta tacaagactt
    11221 acaacaaaag atattagaat caaattttga actagacaaa cttaactcca aagaagctag
    11281 ccctgaggtt aaactacaaa aacaaaagtt aactaaagat atcgctgaag cagaagttaa
    11341 gttatccgaa ctagaaaaga agcgtatcag tattgacgtc aatgcagata acagtaaatt
    11401 caatcgagtg ttaaaagtat ctaaagctag tcttgaagca ttaaataggt ctaaagccaa
    11461 agctattata gacgtggaca atggtgttgc taactctaaa ataaaacgca ctaaagaaga
    11521 gcttaaaagt attccaaaca aaactagatc tcgactagat gtagatacag ggctttctat
    11581 accaactatt tatgcgttta aaaaatcatt agacgcattg ccgaacaaaa aaacaacaaa
    11641 ggtagatgtc gatactaatg gtttaaagaa agcttatgcc tacataataa aagcaaacga
    11701 caatttccaa agacagatgg ggaatttagc taatatgttc cgtgtgttcg gtactgtagg
    11761 ttctaatatg gttggtggat tactaacttc atcttttagt atcttaatac ctgtaatagc
    11821 gagcgtagta cctgtagtat ttgcgctatt aaacgctatc aaagtgttaa ctggcggtgt
    11881 acttgcttta ggtggtgcgg tagcaatagc cggcgctggc tttgtagcat ttggcgcaat
    11941 ggctatcagc gctataaaga tgcttagtga cggcacttta caagctagct cagcaacaaa
    12001 cgaatacaaa aaagctttag atggcgtaaa gtcagcatgg actgatatta taaagcaaaa
    12061 tcaatccgct atattcacaa ctcttgcaaa cggtttaaat actgttaaaa cagcaatgca
    12121 gagcttacaa ccttttttta gtggtatttc aagaggaatg gaagaggcgt ctcaaagtgt
    12181 gtttaaatgg gctcaaaata gcggtgtagc atcaaggttc ttcaacatga tgaatacaac
    12241 tggtgtttcg gtatttaaca agctattaag tgctgcaggc ggtttcggtg atggattagt
    12301 caatgtattc acacaattag caccactgtt tcaatggtcg gctgattggt tggatagatt
    12361 aggtcaatct ttctctaact gggctaatag tgcagctgga gaaaattcga taactcgttt
    12421 tattgaatac acaaaaacaa acttacctat cattggtaat atttttaaaa atgttttcgt
    12481 tggaattaac aatttgatga atgcattcag tggatcatca actggcattt tccaatctct
    12541 tgaacaaatg acagctaatt ttagggaatg gtctgaacaa gtaggacaat ctcaagggtt
    12601 taaagacttt gtcagttata tacaaactaa tggaccacta ataatgcaat taattgggaa
    12661 cattgcaaga ggattagttg cattcgcaac agcgatggct cctatagcta gtgcagtatt
    12721 acgcgttgca gtagcaataa ctggttggat agctaacttg tttgaggcgc atccagctac
    12781 agcacaatta gttggtgtca ttataacttt agttggtgca tttagatttt taataccgat
    12841 tattcttgct gtatctaact ttatgggtgg cggattaata ggtggaatca ttgcgttagt
    12901 aagtaagttc ggtttattaa gagcgggatt aacaatttta aaaggtgcgt tcatgttatt
    12961 aaaaggacca ttaaaaatta tatcagttat attccaattg ttattcggta agattggatt
    13021 aattagaaat gctatcacag gactagtaac tgtgtttggt attttaggtg gtccaataac
    13081 aatagttatc ggtgtaatcg ctgcattaat agctatattc gttttattgt ggaataaaaa
    13141 tgaaggattc agaaacttta ttataaatgc ttggaatgcg ataaaaacgt ttatggttac
    13201 agtttggaat gtgttgaaaa ctgtagcttc ggttgtatgg aatgctattt taaaagctat
    13261 cactacagca gtaactaatg tatacaattt tataatgatt gtttggaatc aaatagccgc
    13321 ttatttacaa gggttatgga atggaattat cgctattgca acaacagtat ggaacctttt
    13381 agttacaatc atcacaactg ttttcacgac gataatgaca atagttatga cgatatggac
    13441 agctatttgg acattcttaa gtacaatctg gaacacgata attacaatcg ctactacgat
    13501 ttggaatttg ttagtcactg taataactac tgtgtttaca acaatcatga ctatcgcaat
    13561 gacaatttgg aatgctattt ggacgttctt acaaacgttg tggaacacta tagttactgt
    13621 ggcaactaag gtttggaacg ctatcactac agctatatct actgcgttac aagcggcatg
    13681 gagttttatt tctaatatat ggaatacgat ttggagtttc ttatctggta tattaacgac
    13741 gatttggaat aaagttgtaa gtatattcac acaagttgta tcaactatat cggacaaaat
    13801 gtctcaagct tggaacttca tcgtgactaa aggtatgcaa tgggtatcta ctataacaag
    13861 tacgctaatt aactttgtta atagagttat tcaaggattc gttaatgttg taaacaaagt
    13921 tagtcaaggt atgacaaatg cagtaaataa aataaaaagc tttataggag attttgtgtc
    13981 tgcaggtgct gatatgatcc gtggtttaat tagaggtatt ggacaaatgg ctggtcaatt
    14041 agtagatgca gctaaaaatg ttgctaagaa agctttagat gcagctaaaa gtgctttggg
    14101 tattcactca ccttcacgtg aattcatgga tgttggtatg tattcaatgc taggtttcgt
    14161 taaaggtata gataatcatt caagtaaagt tatccgtaat gtttctaatg ttgcagataa
    14221 agtagttgat gcatttcaac ctacattaaa cgcacctgac atttctagta ttacaggaaa
    14281 cttaagtaat ttaggtggaa atataaatgc gcaagtacaa cacacacatt ctattgaaac
    14341 atcaccgaac atgaaaactg ttaaagttga attcgatgtc aataacgatg cgcttactag
    14401 tattgttaac ggcagaaatg ctaaacgcaa ttctgagtat tacttataaa ggaggttaca
    14461 aatggacata gaattaacaa aaaaagatgg tactgtaatc aaattaagtg aatacgggtt
    14521 tatcgttaac gatatagtaa ttgatagcat gcaaatcaac acaaagtatc aagacaaaga
    14581 aaatatgaac ggtcgtatat taatggggag caattatatc agtagagata tagttgttcc
    14641 ttgtttttgt aaagttaaaa atcgttcaga cattgcttat atgcgagata tgttgtattc
    14701 gttaacgaca gacatagaac ctatgtattt acgagaaata agaagaaaag aagagttgaa
    14761 ttacaggttt acgcaaccaa cttctgatga ttacgtgaaa ttagataaaa acaacttccc
    14821 ggattatgaa tattcaagac acgatcaaca aatttatgta aatggtaaac agtataaagt
    14881 tatttttaac ggagttataa accctaaaca aaaaggtaat aaagtttctt ttgaactaaa
    14941 attcgaaact acagaattac catacggtga aagtattgga acaagcctag agttagaaga
    15001 aaacaaaaag gttggattgt ggtcgtttga ttttaatatt gattggcatg caggcggaga
    15061 caaaagaaag tatacatttg aaaatttgag caaaggtaca gtttactatc atggtagtgc
    15121 tcctaacgac caattcaaca tgtataaaaa gataacaatt attttaggcg aagatacaga
    15181 atcgtttgta tggaatttaa cgcatgctga aataatgaaa atcgaaggga tcaaactaaa
    15241 agctggagac agaattgttt atgatagctt ccgagtttat aaaaacggtg ttgaaataag
    15301 taccgaaacg aatatagccc aaccaaaatt taaatacgga gctaataaat ttgagtttaa
    15361 tcaaacggta caaaaagttc agtttgattt gaaattttat tataagtagg tgtcagaatg
    15421 acaataacta ttaaaccacc taaaggtaat ggcgcacctg taccagtaga aacaacttta
    15481 gtaaaaaaag ttaacgctga cggtgtatta acttttgata ttctagaaaa caaatatact
    15541 tatgaagtta ttaacgctat agggaaaaga tggattgtta gtcatgtcga aggtgaaaac
    15601 gacaagaaag aatatgtaat aactgtcatt gataggaaat cagaaggcga cagacaactg
    15661 gttgaatgta ctgctagaga gattcctata gacaagttaa tgattgatag gatttatgtt
    15721 aatgtaacag gatcttttac agtagaaaga tattttaaca ttgtgtttca aggtactgga
    15781 atgctttttg aagtcgaagg taaggttaag tcttcgaagt ttgaaaatgg tggcgaaggc
    15841 gatacaaggt tagaaatgtt taaaaaggga ttagaacatt tcggtttaga atataaaata
    15901 acgtatgaca aaaagaaaga cagatataag tttgtattga cgccttttgc aaatcaaaaa
    15961 gcgtcttatt ttatttctga cgaagtcaac gccaacgcta taaaactcga ggaagatgca
    16021 agtgatttcg ccaccttcat tagaggatat ggtaattatt caggagaaga aacattcgaa
    16081 cacgctgggc tcgtaatgga agctagaagt gcattagctg aaatatacgg cgacatccac
    16141 gcagaaccat ttaaagatgg taaagtgact gaccaagaaa ctatggataa agaattacaa
    16201 tcgagattga aaaagtcgtt aaaacaatct ttgtctttgg actttttggt gttaagagaa
    16261 tcatatccag aagcagaccc acaacccgga gacatagtac aaataaaatc taccaaacta
    16321 ggtttgaatg atttagtccg tatagtacaa gttaaaacga ttaggggtat aaacaatgta
    16381 attgttaagc aagatgtaac gcttggtgag tttaatcgag aacaacgata tatgaaaaaa
    16441 gttaatactg cagctaacta tgtttctgga ttaaatgatg ttaacctttc taatcctagt
    16501 aaagcggcag aaaacttgaa gtctaaagta gcgtcaatag ctaaatcaac actcgatttg
    16561 atgagtagaa ctgatttgat tgaagataaa caacagaagg taagctctaa aactgtgact
    16621 acatctgacg gcactatcgt tcatgatttt atagataaat caaacattaa agatgtaaaa
    16681 acaattggaa cgattggcga ttctgtagct agagggtcgc atgcaaaaac taatttcaca
    16741 gaaatgttag gcaagaaatt aaaagcgaaa acgaccaacc ttgcaaaagg tggcgcaact
    16801 atggcaactg ttacagatac aaacaacgtt gaaaatagta tttatagaca agcagaacaa
    16861 attagaggcg acctaatcat attacaaggt acagatgatg actggttaca tggttattgg
    16921 gcaggtgtac cgataggcac tgataaaacg gatacaaaaa cgttttacgg tgccttttgt
    16981 tctgcaattg aagttattcg gaaaaataat ccaacttcaa aaatacttgt aatgacagct
    17041 actagacaat gtcctatgag tggcacaacg atacgtcgta aagatactga taaaaacaaa
    17101 ttagggttaa cgttagagga ttatgtcaac gctcagatat tggcttgtag tgaattggat
    17161 gtaccagtat atgatgccta tcatacagat tattttaagc catataatcc agcgttcaga
    17221 aaatcaagta tgccagacgg attgcatccg aacgagaggg gtcatgaagt tattatgtac
    17281 gaacttatta aaaattatta ccagttttac ggatagaaaa ggaggaagac atggataaca
    17341 aattaattac agacttaagt agagttttcg attacagata tgtagatgaa aatgagtata
    17401 atttcaagct tatttcagac atgctgactg attttaattt ctctcttgaa tatcatagaa
    17461 ataaagaggt atttgcacat aatggagagc aaataaagta tgaacattta aatgttacaa
    17521 gtagcgtctc tgacttttta acatatttaa acggtcgatt tagcaacatg gtactaggtc
    17581 ataacggcga cggtatcaac gaagtaaaag acgcgcgcgt tgataataca ggttatggtc
    17641 ataagacatt gcaagatcgt ttgtatcatg attattcaac actagatgct ttcactaaaa
    17701 aggttgagaa agctgtagat gaacactata aagaatatcg agcgacagaa taccgattcg
    17761 aaccaaaaga gcaagaaccg gaattcatca cagatttatc gccatatact aacgcagtaa
    17821 tgcaatcatt ttgggtagac cctagaacga aaattattta tatgacgcaa gctcgtccag
    17881 gtaatcatta catgttatct agattgaagc ccaacggaca atttattgat agattgcttg
    17941 ttaaaaacgg cggtcacggt acacacaatg cgtatagata cattgatgga gaattatgga
    18001 tttattcagc tgtattggac agtaacaaaa acaacaagtt tgtacgtttc caatatagaa
    18061 ctggagaaat aacttatggt aatgaaatgc aagatgtcat gccgaatata tttaacgaca
    18121 gatatacgtc agcgatttat aatccggtag aaaatttaat gatttttaga cgtgaatata
    18181 aacccactga aagacaactt aagaattcgt tgaactttgt tgaggttaga agtgctgacg
    18241 atattgataa aggtatagac aaagtattgt atcaaatgga tatacctatg gaatacactt
    18301 cagatacaca acctatgcaa ggtatcactt atgatgcagg tatcttatat tggtatacag
    18361 gtgattcgaa tacagccaac cctaactact tacaaggttt cgatataaaa acaaaagaat
    18421 tgttatttaa acgacgtatc gatattggcg gtgtgaataa taactttaaa ggagacttcc
    18481 aagaagctga gggtctagat atgtattacg atctagaaac aggacgcaaa gcgcttttaa
    18541 taggggtaac tattggacct ggtaacaaca gacatcactc aatttattcc atcggccaaa
    18601 gaggtgttaa ccaattctta aaaaacattg cacctcaagt atcgatgact gattcaggcg
    18661 gacgtgttaa accgttacca atacagaacc cagcatatct aagtgatatt acggaagttg
    18721 gtcattacta tatctatacg caagacacac aaaatgcgtt agatttcccg ttaccgaaag
    18781 cgtttagaga tgcaggttgg ttctttgatg tactgcctgg acactataat ggtgctctaa
    18841 gacaagtact taccagaaac agcacaggta gaaatatgct taaattcgaa cgtgtcattg
    18901 acattttcaa taagaaaaac aacggagcat ggaatttctg tccgcaaaac gccggttatt
    18961 gggaacatat ccctaagaat attacaaaat tatcagattt aaaaatcgtt ggtttagatt
    19021 tctatatcac tactgaagaa tcaaaacgat ttactgattt tcctaaagac tttaaaggta
    19081 ttgcaggttg gatattagaa gtaaaatcga atacaccggg taacacaaca caagtattaa
    19141 gacgtaataa cttcccgtct gcacatcaat ttttagttag aaactttggt actggtggcg
    19201 ttggtaaatg gagtttattc gagggaaagg tggttgaata atgatagtag ataatttttc
    19261 gaaagacgat aacttaatcg agttacaaac aacatcacaa tataatccaa ttattgacac
    19321 aaacatcagt ttctatgaat cagatagagg aactggtgtt ttaaattttg cagtaactaa
    19381 gaataacaga ccgttatcta taagttctga acatgttaag acatctatcg tgttaaaaac
    19441 cgatgattat aacgtagata gaggcgctta tatttcagac gaattaacga tagtagacgc
    19501 aattaatggg cgtttgcagt atgtgatacc gaatgaattt ttaaaacatt caggcaaggt
    19561 gcatgctcag gcattcttta cacaaaacgg gagtaataat gttgttgttg aacgtcaatt
    19621 tagcttcaat attgaaaatg atttagttag tgggtttgat ggtataacaa agcttgttta
    19681 tatcaaatct attcaagata ctatcgaagc tgtcggtaaa gactttaacc aattaaagca
    19741 aaatatggct gatacacaaa cgttaatagc aaaagtgaat gatagtgcga caaaaggcat
    19801 tcaacaaatc gaaatcaagc aaaacgaagc tatacaagct attactgcga cgcaaactag
    19861 tgcaacacaa gctgttacag ctgaagtcga taaaatagtt gaaaaagagc aagcgatttt
    19921 tgaacgtgtt aacgaagttg aacaacaaat caatggcgct gaccttgtta aaggtaattc
    19981 aacaacaaat tggcaaaagt ctaaacttac agatgattac ggtaaagcaa ttgaatcgta
    20041 tgagcagtcc atagatagcg ttttaagcgc agttaacaca tctaggatta ttcatattac
    20101 taatgcaaca gatgcgccag aaaagacgga tataggcacg ttagagaagc ctggacaaga
    20161 tggtgttgat gacggttctt cgttcgatga atcaacttat acatcaagca aatctggtgt
    20221 gttagttgtt tatgttgttg ataataatac tgctcgtgca acatggtacc cagacgattc
    20281 aaacgatgag tacacaaaat acaaaatcta cggcacatgg tacccgtttt ataaaaagaa
    20341 tgatggaaac ttaactaagc aatttgttga agaaacgtct aacaacgctt taaatcaagc
    20401 taagcagtat gtagatgata aattcggaac aacgagctgg caacaacata agatgacaga
    20461 ggcgaatggt caatcaattc aagttaactt aaataatgcg caaggcgatt tgggatattt
    20521 aactgctggt aattactatg caacaagagt gccggattta ccaggtagcg ttgaaagtta
    20581 tgagggttat ttatcggtat tcgttaaaga tgatacaaac aagctattta acttcacacc
    20641 ttataactct aaaaagattt acacacgatc aatcacaaac ggcagacttg agcaacagtg
    20701 gacagttcct aatgaacata aatcaacggt attgttcgac ggtggcgcaa atggtgtagg
    20761 tacaacaatc aatctaactg aaccgtacac aaactattct attttgttgg taagtggaac
    20821 ttatccaggt ggcgttattg agggattcgg acttaccgca ttacctaacg cgattcaatt
    20881 gagtaaagcg aatgtagttg actcagacgg caacggtggc ggtatttatg agtgcttact
    20941 atccaaaaca agtagcacta ctttaagaat agataacgat gtgtactttg atttaggtaa
    21001 aacatcaggt tctggagcga atgccaacaa agttactata actaaaatta tggggtggaa
    21061 ataatgaaaa tcacagtaaa cgataaaaac gaagttatcg gattcgttaa tactggcggt
    21121 ttacgcaata gtttagatgt agatgataac aatgtgccta ttaaatttaa agaagagttc
    21181 gaacctagaa agtttgtttt cactaacggc gaaattaaat acaatagcaa tttcgaaaaa
    21241 gaagacgtac cgaatgcatc aaaccaacaa agtgcgtcag atttaagtga tgaggaactt
    21301 cgcggaatgg ttgcgagtat gcaaatgcag gtggcacaag taaacgtatt aacaatggaa
    21361 ttagctcaac aaaacgctat gttaacacaa cagttgactg aactgaaaac taacaaaaca
    21421 agtactgagg gggacgttta aataatgaag atgatttatc caacttttaa agacattaaa
    21481 actttttatg tttggggtta ctataaaaac gagcaaatta agtggtacgt agacaagggt
    21541 ttaatcgata aagaagaata cgctttaatc actggagaaa aatatccaga aacaaaagat
    21601 gaaaagtcac aggtgtaatg cttgtggctt tttaatttga ataaagtggg tggcataatg
    21661 tttggattta ccaaacgaca tgaacaagat tggcgtttaa cgcgattaga agaaaatgat
    21721 aagactatgt ttgaaaaatt cgacagaata gaagatagtc ttagagcgca agaaaagatt
    21781 tatgacaaat tagatagaaa ttttgaagaa ttaaagcgcg acaaggtaga agatgaaaag
    21841 aataaagaaa agaatgccaa gaatattaga gacataaaaa tgtggattct aggtttgata
    21901 gggactatct tcagtacgat tgtcatagct ttactaagaa ctatttttgg tatttaaagg
    21961 aggtgattac catgcttaaa gggattttag gatatagctt ctgggcgtgc ttctggtttg
    22021 gtaaatgtaa ataacagtta agagtcagtg cttcggcact ggctttttat tttgattgaa
    22081 atgaggtgca tacatgggat tacctaatcc gaaaaataga aagcccacag ctagtgaagt
    22141 ggttgaatgg gcgttatata tcgctaaaaa caaaatagct attgatgtac ctggttctgg
    22201 aatgggagca caatgctggg atttacctaa ttatttactc gataaatatt gggggtttag
    22261 aacatgggga aatgctgatg ctatggctca gaaatctaat tatagaggta gagatttcaa
    22321 gataattaga aatacaaaag attttgtacc acaaccaggc gactggggtg tttggactgg
    22381 tggttgggca ggacatgtaa acattgtagt gggaccatgc acaaaagact attggtatgg
    22441 tgtggatcaa aactggtata caaataatgc aacaggaagt ccgccgtata aaatcaaaca
    22501 ctcttatcat gatggaccag gtggaggagt taaatatttt gttagaccac catatcatcc
    22561 ggagaaatct acgccggcac ctaaacccga agacgacagt gataataacg aaaaaaataa
    22621 taaaaaagtt ccgatttgga aagatgtaac aactataaag tacacaattt ctagtcaaga
    22681 agttaattat ccagaatata tttatcattt tatagtagag ggtaatcgac gactcgaaaa
    22741 acctaaagga ataatgatta gaaatgctca aacaatgagt tcagtagaaa atttatataa
    22801 cagtaggaag aaatacaaac aagatgtgga atatccccac ttttatgtag atagacataa
    22861 tatttgggct cctagaagag cggtatttga ggttcctaat gaacctgatt atatagttat
    22921 agacgtatgt gaagattata gtgcgagtaa aaacgaattt attttcaatg aaattcacgc
    22981 aatggttgta gctgtagata tgatgatcaa atatgagata cctctaagta ttgaaaattt
    23041 aaaagtagac gacagcattt ggcgttctat gttggaacat gttaattgga atatgattga
    23101 caacggtgct ccccctaaag ataaatacga agcattagaa aaggcattat ttaatatatt
    23161 taaaaacaga gaaaaattat taaattctat aactaaacca acagtaacaa aatctagaat
    23221 aaaagttatg gtagataata aaaacgctga tatagcgaat gtaagagact catcaccaac
    23281 agctaacaat ggctcggcat ctaaacaacc gcagattata actgaaacga gcccttatac
    23341 attcaaacaa gcactggata aacaaatggc aagaggtaac ccgaaaaaat ctaatgcttg
    23401 gggctgggct aacgctacac gagctcaaac gggctcggca atgaatgtta aacgaatatg
    23461 ggaaagtaac acgcagtgct accaaatgct taatttaggc aagtatcaag gtgtttcagt
    23521 tagttcactt aataagatac ttaaaggtaa ggggacattg aataatcaag gtaaagcgtt
    23581 cgcagaagct tgtaaaaagc acaacattaa tgaaatttat ttaatcgcgc atgctttctt
    23641 agaaagtgga tatggaacaa gtaacttcgc taacggaaaa gatggagtat acaactactt
    23701 cggcattggc gcttacgaca acaatcctaa ctacgcaatg acgtttgcaa ggaataaagg
    23761 ttggacaact ccagcaaaag caatcatggg cggtgctagc ttcgtaagaa aggattacat
    23821 caacaaaggg cagaatacac tgtaccgaat tagatggaat cctaaaaatc cagctacaca
    23881 tcaatatgct actgctatag agtggtgcca acatcaagca agtacaatcg ctaagctata
    23941 taaaaaaatc ggcttaaaag gtatctactt tataagagat aaatataaat aaagaggtgt
    24001 ataaatgtac aaaataaaag atgttgaaac gagaataaaa aatgatggtg ttgacttagg
    24061 tgacattggc tgtcgatttt acactgaaga tgaaaataca gcatctataa gaataggtat
    24121 caatgacaaa caaggtcgta tcgatctaaa agcacatggc ttaacaccta gattacattt
    24181 gtttatggaa gatggctcta tattcaaaaa tgagcccctt attatcgacg atgttgtaaa
    24241 aggattcatt acctacaaga tacctaaaaa ggttatcaaa cacgctggtt atgttcgttg
    24301 taagctgttt ttagagaaag aagaagaaaa aatacatgtc gcgaactttt ctttcaatat
    24361 cgttgatagt ggtattgaat ctgctgtagc aaaagaaatc gatgttaaat tggtagatga
    24421 tgctattacg agaatcttaa aagataacgc gacagattta ttgaacaaag actttaaaga
    24481 gaaaatagat aaagatgtca tttcttacat cgaaaagaat gaaagtagat ttaaaggtgc
    24541 gaaaggtgat aaaggcgaac cgggacaacc tggtgcaaaa ggtgaagcag gtaaaaaagg
    24601 agaacaaggc gcacccggta aaaacggtac tgtagtatca atcaatcctg acactaaaat
    24661 gtggcaaatt gatggtaaag atacagatat caaagcagaa cctgagttat tggataaaat
    24721 caatatcgca aatgttgaag ggttagaaga taaattgcaa gaagttgaaa aaatcaaaga
    24781 tacaactctc aacgactcta aaacgtatac ggattcaaaa attgctgaac tagttgatag
    24841 cgcgcctgaa tctatgaata cattaagaga attagcagaa gcaatacaaa acaactctat
    24901 ttcagaaagt gtattgcaac agattggctc aaaagttagt acagaagatt ttgagagatt
    24961 caagcaatca ttaaacagtt tgtatgcaga taaaaatcat agtcatacaa tcaaacagat
    25021 tgaaggatta gaaaatgctt tatcaaaaaa atcagacata aatcacagtc atgatgaacg
    25081 ttatctttta tcatcaaatg cttttacaaa agaggaagca gataaacttt atcaacctat
    25141 cggttcttcg cagccgtcac tgaatatttg gacaggcagt gaaacagaat ataattattt
    25201 gtatcaaaaa gaccctaata cactttactt aattaagggg tgatttttat ggaaggtaat
    25261 tttaaaaatg taaagaagtt tatttacgaa ggtgaagaat atacaaaagt atatgctgga
    25321 aatatccaag tatggaaaaa gccttcatct tttgtaataa aacccttacc taaaaataaa
    25381 tatccggata gcatagaaga atcaacagca aaatggacaa taaatggagt tgaacctaat
    25441 aaaagttatc aggtgacaat agaaaatgta cgtagcggta taatgaggat ttcgcaaact
    25501 aatttaaggt caagtgattt aggaatatca ggagtcaata gcggagtggc aagtaaaaac
    25561 atcaacttta gtaatccttc agggatattg tatgtcacta taagtgatgt ttattcagga
    25621 tctccaacat tgaccattga ataattttaa acgactaatt ttttagtcgt tttttatttt
    25681 ggataaaagg agtaaacaaa tggatatcgg tacaatcgta agaacaattt tattaatagt
    25741 cgcatggatc aatcagtttt tagcaatcaa acatatttct ccaatcccag ttgacgaagt
    25801 gtttataagc acagtcgtta ctgggattgt ttcaatttgg acgtggtgga agaataacaa
    25861 ctttactcac gcatctaaga aagggcaaca aaaaatttat gaagtaaaag ctggcattca
    25921 gtcaactggt ggcgcaccta aagtgaacgg agatgataac aatgccgtcg gttaggacat
    25981 acagtcaagc tattagttat cttaaaagtt tagagggtaa ggcgtggaat ccagacaatg
    26041 catttggatg tcaatgcttc gatactgcta accaatattg gctttactta tttaatcaca
    26101 ggttgaaagg tgtgggcgct gcagacattc caacatggaa tgatttcact aacgaggcaa
    26161 ccgtttacga aaatactgtg tcgtttcaag cattgcctgg cgacgtcgtt atttttaacc
    26221 gtaattatgg tggtggttat ggtcatgtag gtattgtaat aagcgctacg ttagattcta
    26281 taactatttt agagcagaac tggctaggcg gtgcttactg gagtccacca gaagttacta
    26341 caagacgcac acacggctac gacttcccta tgtggtttat ccgtccattc tacgcaaaag
    26401 aaacgaccgc taataagcta agaagcgcag tgaagccagt taaacaagat aagttatcaa
    26461 aaggtaaaaa aatcatgctt gtggctggtc atggtattgg tgcatactct aacgacccag
    26521 gtgccgttgc gaatggagaa aacgaaagag attttaaccg taaaaatatt atacctagag
    26581 tgaaaaagta tcttgagtca gtaggcaaca cagtattgtt atacggtggc aactcgatga
    26641 atcaagattt atatcaagat acattgtacg gtcaacgtgt tggaaactat aaagattatg
    26701 gcatgtactg gattaaaagt gaagtcaaac cggatgcaat catagagttt catttagatt
    26761 ctgctagccc acaagcaagt ggcgggcatg taatcattag cgatcgtttc ccagctgatg
    26821 acattgacaa ggcattaagt agtgcattag ataaaacagt gggtaaaata agaggtgtga
    26881 cacctagagg ggatttattg aacgctaacg tgtctgctga tcttaatctt aattatcgtt
    26941 taatcgaatt aggttttatc acatctacga aagatttaaa ctacattaaa aacaatttag
    27001 acagcttcac gaagcggatt gctgaagcca ttaacggcag acaaattgat gcgccaagta
    27061 gtaagccaag cgctgacaaa ataacatgga attggaaagg cgtattttat cctaatccag
    27121 aaaaagctat aagagtcaga aaaacagctg gattaaccgg cacagtcgtt gaagaagatt
    27181 catggctata cacaaaagat gattgggtaa aattcgacca agtcattaaa aaagatggct
    27241 actggtggat tagattcaaa tatcaacgtg agggctctag tactaacaat ttctattgtg
    27301 cagtgtgtag aattactgat aaggaacaaa agattaaaaa tgaaaaatat tggggcacga
    27361 ttgagtgggc ttaataggtt gtacctataa aaagaaaaga ggtaggttat tttcttccta
    27421 cctctaaaaa tgattatctt tctattgtta tatgagttat atctttagga ctaatcagtc
    27481 tattttttac attagaatct tgatctccta ccttgccata tactttttca tcagaaggat
    27541 ctttactatg gatagttact ttatcaccga ctttaacaat atgcttttct tttaatttat
    27601 ctactaattt tttccatgca tcatttgcct ctattgtgtt tccgtttgga ttaactcttg
    27661 taatatcgac acggttaacg ctatcaggac taacggtgct gttattagta ttactaagat
    27721 tatctaagtt cgcagtccca gaaatttcgc tctctccacc gttttttaat ttatatttta
    27781 ctttaatcgt ttctttgtct gttttatcaa tgatatttgc gtcttttaaa gcgtctctta
    27841 catttttcca caattcgcta tctgttattt cagaagcttt tgcaacgtta ttaataccat
    27901 tataatttga agaagaatga aaacctgaac ctactgttgt taaaactaaa gcacttgcta
    27961 tcaatgtttt tgttaatagt tttttattca ttttattttc tcctataact tatttgcaat
    28021 cgattacaaa gtaattttag aattattatt tatgtaaatc aattaaataa ttattaacaa
    28081 atccataaaa ttttatcatt gaaatataat aattttgagc tagaaatatt cgtcatttat
    28141 gctataatcg ttttagacac agcaatgtgt tcaaattttc atctattcgt aagttagcct
    28201 tcgggctgac tttttatttc cattattcac atgttaatct tgttgttgtt taggcaggta
    28261 cttcggtact tgcctatttt tttatgcaaa ttttaaaaaa cacttgacta ataaacattt
    28321 gtttagtata attatatttg taggttagtt gatgacttac aaattatgtg taaggaggtg
    28381 aaaagcctca tgctagacat aataaaaaca cttctagaac atcaagtatt ggcagtactg
    28441 ataattccag aagtgttaaa acaacttaga gaatggcatc tcggctacct agaccgaaag
    28501 ccaaacaaca aagattaaca ttatgcttgg agcctgatgg ctcctcctta cacttatata
    28561 atataatatt atttggaggt tttcaattat gacagaacaa atgtatttaa tattgttttt
    28621 attaagccta ccattgttat tatttatcgg gagaaaaaca catttttatt gtttagataa
    28681 aaagaatgga cgtagataat atgagtgatt ataaattaaa aataattgaa ttgatcaaaa
    28741 gtgatataac aggttaccaa attcacaaac aaactggcgt agcgcaatat gtaatttcac
    28801 aattaaggca aggaaagcgc gaagtagata acttaacttt aaatacaact gaaaaactat
    28861 acagttacgc acgacaagtg ttataatata aatgtgaaat ggtcattctt gaaatgactc
    28921 ggtcgctact ggcacagacc gtttaaagtg tcaccacaac atgaactgag aattcatatg
    28981 acgttgctga cgagcgacaa agctctgtgt tcctggatgg gagtaagttt gtgtggtggt
    29041 gcataacaag tcgctgaaat atttgcgaca taataaagca tattatcggt tttattaagt
    29101 gctaaaggca catcttaacc acccatacta gttactgggt ggttgttttt tatgttatat
    29161 tataaatgat caaaccacac cacctattaa tttaggagtg tggttatttt aatatatgaa
    29221 gctaaaataa ctacaaatga taccattttt gataccaaaa aataataacc tcaaaatatc
    29281 gagagaaata acttcatttt aaatcgcatt aaatcaatgt ttctataaaa ataagtcctt
    29341 aaaaattagt tttttcaatc gaaatggaag gtagtattgg atagctttaa accgcgttgt
    29401 taagccattc ttgacttccg gaaatgggta ttgataccat tttgatactg aatataacaa
    29461 aaagccacat tactgtggct ttttttgttt tataactaaa tcggattgat agataagctt
    29521 tgtacttatt tatatcagtc cgattttttg attggtgtaa aaaataatca ttgatggtgg
    29581 ataaagcgac aacacaaata caatatgatt gtggcattag agtgctggtc tttattaaat
    29641 taattgaaaa ctacatcaaa tattctttaa agataattcg atattagttc gattaagatt
    29701 cgttgtataa gtgagttaaa ataagaaaac tattaataat attaagttca ctacagatgt
    29761 tgctaatgga ccataagttt taaagacatc ttcactttta taaccaacaa tcgcatctaa
    29821 aaattgaact aaaatcattg caatggatat agttatcaaa aatatagcac tatgaatgac
    29881 taaagaaaaa atagctaata aaaataaagg taagcttcga ctaagtgcat aatatgcatt
    29941 tatattatga ctagatgcac atgcttgaat tgaataacct aaacttacac tggcactaat
    30001 gattgtaaat attgctaaaa caaaatacat gttaatcctt ctttctatat ttgaatataa
    30061 acaagtactt gtctaaagat atttaaaaga taattagaat aaatttatga gaaactggtt
    30121 gttatcatta taatggtttc aaatgattat aactatgtca taaactgaat ttgttgaaat
    30181 ttttcattat gtaaatttat taataacaaa cagctcgaac tatagcatca ttttactaat
    30241 gaatgcatta aagtaactat gattaaaaat gcatattaat tatcattatt aagtctatta
    30301 tatataatga attttaactg gtttattaaa cgagaacgtc gggaattaag taactacaat
    30361 aaaaataaga tatgacaata aggagactac acgcgtgatc attgccataa ttatattgat
    30421 atttatttcg tttttctttt caggaagcga gacggcatta acggctgcca ataaaacaaa
    30481 atttaaaact gaagctgaca aaggtgataa aaaagcaaaa ggcattgtaa agttacttga
    30541 aaaaccaagt gagtttatta caacgattct aattgggaat aatgtcgcga atattttatt
    30601 accaacactt gttacaatta tggctttacg ttgggggatt agcgttggta ttgcatcagc
    30661 tgttttaaca gttgttatca ttttgatttc cgaagtgatt cccaagtctg tcgctgcaac
    30721 atttccagat aaaataacaa ggcttgtata tccaattatt aatatttgtg tcattgtgtt
    30781 tcgccctatc acattacttt taaataagtt gacggacagt attaatcgaa gtttatctaa
    30841 gggtcaacct caagaacatc aattttcaaa agaagaattt aaaacaatgt tagcaattgc
    30901 tggacatgaa ggtgctttaa atgaaattga gacgagtagg ttggaaggtg tcattaattt
    30961 tgaaaattta aaagtaaaag atgtagatac aacacctaga attaatgtga cggcatttgc
    31021 ttcaaatgcg acatacgaag aagtttatga aacggttatg aataagccat acactagata
    31081 tccagtgtac gagggagata ttgataacat tattggggtg tttcattcta aatatctgtt
    31141 ggcttggagt aataaaaaag aagatcaaat tacaaactat tcagctaagc cattatttgt
    31201 gaatgaacac aataaagctg aatgggtatt acgtaagatg actatttcta gaaaacattt
    31261 agcaattgtg ttggacgaat ttggtggtac cgaagcgata gtgtcacatg aagacttaat
    31321 tgaagaatta ttaggtatgg aaattgaaga tgagatggat aaaaaggaaa aagaaaaact
    31381 ttctcaacag caaattcaat ttcaacaacg gaaaaatcgc aacgtatcta tataaggagc
    31441 gaacagctat gtggaataag aatcgactta ctcaaatgtt aagtattgaa tatccaatta
    31501 tacaagcagg tatggcagga agtacgacac cgaaattagt tgcatcagta agtaacagtg
    31561 gtgggttagg cacaataggc gcaggttact ttaatacgca gcaattggaa gatgaaatag
    31621 attatgtacg ccaattaacg tcaaattctt ttggcgtaaa tgtctttgta ccaagtcaac
    31681 aatcatatac cagtagtcaa attgaaaata tgaatgcatg gttaaaacct tatcgacgcg
    31741 cattacattt agaagagccg gttgtaaaaa ttatcgaaga acaacaattt aagtgtcata
    31801 ttgatacgat aattaaaaag caagtgcctg tatgttgttt tacttttgga attccaaatg
    31861 aatctattat agaaagattg aaagaagcaa acattaagct aataggtaca gcaacaagtg
    31921 ttgatgaagc tattgcgaat gaaaaagcgg gtatggatgc tatcgttgct caaggtagtg
    31981 aagcaggtgg acatcgtggt tcatttttaa aacctaaaaa tcaattacct atggttggaa
    32041 caatatcttt agtgccacaa attgtagatg tcgtttcaat tccggtcatt gccgctggtg
    32101 gaattatgga cggtagagga gttttggcaa gtattgtctt aggtgcagaa ggggtacaaa
    32161 tgggcactgc atttttaaca tcacaagata gtaatgcatc agaactacta cgagacgcaa
    32221 ttataaatag taaagaaaca gatacagtcg ttacaaaagc gtttagtgga aagcttgcac
    32281 gcggtatcaa caataggttt atcgaagaaa tgtcccaata cgaaggcgat atcccagatt
    32341 atccaataca aaatgagcta acaagtagca taagaaaagc cgcagcaaac atcggcgaca
    32401 aagagttaac acatatgtgg agtggacaaa gcccgcgact agcaacaacg catcccgcca
    32461 acaccatcat gtccaatata atcaatcaaa ttaatcaaat catgcaatat aaataatcga
    32521 ccgcaatcca caaaagcaca agcactccca aacattattt tagtgcttgc catttttgtg
    32581 gattgcgttt ctattttacc aatctagtca aacgaaatta tcaaactaaa gttcgctgaa
    32641 atgattatag tcaaacgaaa acatcaaact aaagctcgct gaaatgatta tgatcaaacg
    32701 aaattatcga gctgaaggtc accgaaatga ttataattaa acgaaattat cgagctaaag
    32761 ttcactgaaa tgattataat caaacgaaaa catcaagctg aagatcgccg aaagatttta
    32821 atcaagcgaa aacatcaaat taaagttcgc tgaaatgatt atgataaaag ttatatggta
    32881 tgatgacatt ggtgatatat atgataaaca tcggattaac aggttggggt gatcactatt
    32941 cattatatga agatttagaa cgccaaaccg ataaacttaa aacatatgct ggacattttc
    33001 cggttgtcga attagatgcg acatactatg cgatacaacc ggaaagaaat atattgaaat
    33061 ggataaaaga aacgcctgat acatttgaat ttgtggtcaa aattcatcaa gcactcacat
    33121 tgcatgcaga ctacaaaaca tttgcagata caaggcaaga actatttgat caatttaaga
    33181 atatgttaga gcccttacat acacagaaaa aattagcaat ggtattggtt caatttccgc
    33241 catggtttga ctgcaatgca caaaatatca aatatatttt gtatgtaaga cagcaattac
    33301 aagcatttcc aatgtgtgta gaatttaggc atcaatcatg gtttagtgat gcatttaaag
    33361 aacaaacatt ggcattttta acagaacatc aaatcattca tgcagtagtt gatgaaccac
    33421 aagtgaaaga tggcagtgta cctttagtca atcgaatcac aaatgaaatt gcgtttgtac
    33481 gttatcatgg acgtaatcat tacggttgga ctaagaaaga tatgtcagat caagaatggc
    33541 gagatgtacg ctatttatat gattataatg agcaagaatt aatagacttg gcacaaaagg
    33601 cacaaatatt agcacaaaaa gctaagaaag tttacgtcat atttaacaat aattctggtg
    33661 gtcatgcagc aaataatgcc aaaacatatc agcgattatt gaatatagaa tatgaagggt
    33721 tagcaccaca acaattaaaa ttattttaag aggcgacgac tatgttatta acaattacat
    33781 tattagtttt aatcggaggt ttgtcagcga ttatagggtc tatcgtaggc attggaggcg
    33841 gtattattat cgttccaaca atggtttacc tcggtgttga acatggatta ctacataata
    33901 ttacaacaca agtagcgata gggacgtctt cagtcattct aattgtgaca ggactttctt
    33961 catcacttgg atatttaaaa acaaaacaag ttgatattaa aaatggttcc atctttttat
    34021 ttggactatt accaggttca ttgcttgggt ccttcattag tagatattta acatttgagt
    34081 catttaattt atattttggt atctttttaa ttttcgtagc cattttatta atggtaagaa
    34141 ataagattaa accgtttaaa attttcgata aacccaagta tgaaaagact tatgtagacg
    34201 ctaaaggtaa aacatatcat tatagtgtgc caccattgtt tgcttttatt acaacgtttt
    34261 taattggtat attgacaggt ttatttggta ttggaggtgg cgcactaatg acgccactaa
    34321 tgcttattgt atttagattt ccacctcatg tagctgttgg aacaagtatg atgatgattt
    34381 tcttttcaag tgtcatgagt tctatagggc acattgctca aggtcacgta gcttggggtt
    34441 atgcaatcat tttaattatt tctagttatt ttggtgcgaa aatcggtgtc aaagtgaatc
    34501 aatcaattaa gtcagatacg gtagtaacat tattgagaac agtaatgttg ttaatgggta
    34561 tatatttaat tattcgagcg ttgatttaat acaactttaa aaggaggacg tcaatttgag
    34621 gcttacaatt tatcatacga acgatattca tagtcattta catgaatacg aacgcattaa
    34681 agtatatatg gcagaacaac ggccacgact taatcatcct tctttatatg ttgatctagg
    34741 tgatcatgta gatttatccg cacctataac tgaagcaact ttaggtaaaa agaatgtggc
    34801 attactaaat gaagcaaaat gtgatgttgc aacaatcggt aataatgaag ggatgaccat
    34861 ttcacacgaa gctttaaatc acctttacga cgaagcaaaa tttatagtga catgtagcaa
    34921 tgttatagat gaatcaggtc atttaccaaa taatatcgtt tcttcttata ttaaggatat
    34981 ggacggtgtg aaaatactat tcgttgcagc gacagcacct tttaccccat tttatcgtgc
    35041 actagattgg attgttaccg atccacttga atctataaaa gaagaaatta aacatcaacg
    35101 aggtaaattt gatgtaataa tcgtgctaag tcattgtggc attttcttcg atgaaacatt
    35161 atgccaagaa ttgcctgaaa ttgatgtcat ttttggtagt catacgcatc attattttga
    35221 acatggtgag atcaataatg gtgtactgat ggcggcagct ggaaagtatg gtaattatct
    35281 tggagaggtt aatttaactt ttgaggcaca taaagtagta cataaaactg caaagattat
    35341 tcctttagaa acattacctg aagttgaaac ttcatttgaa gaagaaggaa aaacgttaat
    35401 gtccaattca gtaattcaac atccagtagt gcttaagcgt agtatgaatc acataactga
    35461 agctgcatac ttattagctc aaagtgtttg tgagtataca catgcacaat gtgccatcat
    35521 caatgctggc ttactcgtta aagatattgt aaaagatgaa gtgacagaat atgacattca
    35581 tcaaatgtta ccgcatccga ttaatatggt aagggttaga ctttctggtg tgaaattaaa
    35641 agagattata actaaaagta ataaacaaga atatatgtat gaacatgcac aaggtttggg
    35701 tttcagaggg aatatatttg gaggatatat tctttataat ttagggtaca ttcattctac
    35761 agggcgttac tatctgaatg gagaagaaat cgaagacgac aaagaatatg tattaggtac
    35821 gatagacatg tatacgttcg gtcgttattt cccaacactg aaagaattac caaaagagta
    35881 tttaatgcca gagtttttaa gagatatatt taaagaaaaa ttattggaat attaaaaagt
    35941 aagattattg gattttcatt tgtcatgaat ttcgatataa tgtttaaaga tacacttaac
    36001 aggagggtat gtgttgttat ggcgacaaaa aacgaggaaa tattacgtaa accggattgg
    36061 ttgaaaataa aattaaatac caacgaaaac tatacaggac ttaagaagat gatgagggaa
    36121 aaaaatctta atactgtatg tgaagaagct aaatgtccta atatacatga atgttggggt
    36181 gcacgtcgta cagcgacatt tatgatttta ggtgccgtat gtacaagagc ttgtcgtttt
    36241 tgtgcggtta agacaggttt acctaatgaa cttgatttaa atgagcctga acgtgtagct
    36301 gaatcagttg aattaatgaa tttgaaacac gttgttatca ctgctgttgc gcgtgatgat
    36361 ttaagagatg ctggttcaaa tgtttatgct gagacagtac gtaaagttag agaaagaaat
    36421 ccatttacaa cgattgaaat tttaccatca gatatgggcg gggactatga tgcgttagaa
    36481 acattaatgg cgtcaagacc tgacatttta aaccataata ttgaaactgt tcgtcgctta
    36541 acaccgagag ttcgtgcgcg tgcgacttac gacagaacat tagagttttt acgtcgttca
    36601 aaagaattac aaccagatat cccaactaaa tcaagtatta tggttggatt aggtgaaact
    36661 atagaagaaa tttatgaaac gatggatgat ttacgtgcga atgatgtaga tattttaacg
    36721 attggtcaat atttacaacc ttcacgtaaa catttaaagg ttcaaaaata ttacacgcct
    36781 ttagagtttg gtaaattaag aaaagtggca atggataaag ggtttaaaca ttgccaagct
    36841 ggacctttag tacgtagttc ttatcatgcg gatgagcaag taaatgaagc tgctaaagaa
    36901 aagcaacgcc aaggtgaggc acagttaaat agttaatatt taacaattaa taaggcataa
    36961 aggcttagtt tgtacaaaac gaacgtgtca tagaagtaat cattattttt atgaaacact
    37021 aaaagtataa actgagcctt tcattgtcat agataggtga agaatttgat aaaagtagat
    37081 caacattact ttgaattaat agaaaattat cgcgaatgtt ttaatgaaga acaatttatt
    37141 gctaggtatt cagatatttt agacaaatat gattacattg ttggtgacta tggttacgat
    37201 caattacgat taaaaggctt ttacaaagat tctaataaaa aagcagagat gagtaaacgt
    37261 ttttcaaata ttcaagatta catatttgaa tattgtaact ttggttgtcc ttactttgta
    37321 ttaagacatt tgtctaaaca agaggttaaa aagttaatcg aagaagttca tccgtctgat
    37381 gtgatagatg acgacaataa acttcaagat gtgaagatta agccaaccat tcaagatact
    37441 gaacattaat aaaaccctta gctagattga aaatgggaat catgcaattc aagcatggac
    37501 ctgtaatcta gttaggggtt tttatcttta at
//
LOCUS       DAOBBH010000005        37478 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.005, whole genome shotgun sequence.
ACCESSION   DAOBBH010000005 DAOBBH010000000
VERSION     DAOBBH010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 37478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37478
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.005"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            72..1190
                     /gene="ald"
                     /locus_tag="P6L01_000166"
     CDS             72..1190
                     /gene="ald"
                     /locus_tag="P6L01_000166"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HDP3029268.1"
                     /translation="MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSG
                     FSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIV
                     EKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGV
                     HENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLEDMYAEKDVTV
                     VKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTI
                     ETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAI
                     KDNEALSTGVNIYQGQVTNQGLASSHDLDYKEILNVIE"
     gene            1285..2325
                     /gene="tdcB"
                     /locus_tag="P6L01_000167"
     CDS             1285..2325
                     /gene="tdcB"
                     /locus_tag="P6L01_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HDP3029269.1"
                     /translation="MTTNTVTLQTAHIVSLGDIEEAKASIKPFIRRTPLIKSMYLSQN
                     ITKGNVYLKLENMQFTGSFKFRGASNKINHLSDEQKAKGIIGASAGNHAQGVALTAKL
                     LGIDATIVMPETAPIAKQNATKGYGAKVILKGKNFNETRLYMEELAKENGMTIVHPYD
                     DKFVMAGQGTIGLEILDDIWNVNTVIVPVGGGGLIAGIATALKSFNPSIHIIGVQAEN
                     VHGMAESFYKRALTEHREDSTIADGCDVKVPGEKTYEVVKHLVDEFILVSEEEIEHAM
                     QDLMQRAKIITEGAGALPTAAILSGKIDKKWLEGKNVVALVSGGNVDLTRVSGVIEHG
                     LNIADTSKGVVG"
     gene            2356..3678
                     /locus_tag="P6L01_000168"
     CDS             2356..3678
                     /locus_tag="P6L01_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDP3029270.1"
                     /translation="MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAG
                     MALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYF
                     PANVAALSIVFATQLINLFHLSIGSLIPIAIASALSIVLINFLGSKAGGILQSVTLVI
                     KLIPIIVIVIFGIFQSGDITFSLIPITGNSGNGFFTAIGSGLLATMFAYDGWIHVGNV
                     AGELKNPKRDLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTSKILFG
                     ENGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGAPWFGA
                     IIQLIIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYKVPLYP
                     IIPLIAILAGSFVLINTLFTQFILAIIGILITALGIPVYYYKKKQKAA"
     gene            3834..5225
                     /gene="norB"
                     /locus_tag="P6L01_000169"
     CDS             3834..5225
                     /gene="norB"
                     /locus_tag="P6L01_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorB"
                     /protein_id="HDP3029271.1"
                     /translation="MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSF
                     NTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIP
                     LLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
                     VATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLTLNI
                     LITKGSELGVTSLLFITLLAIAIGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASN
                     FLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKP
                     MLIGTGVLIVGECLISLTFLPEILYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLE
                     KVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVI
                     ILLLVPKQNDTQL"
     gene            5623..>37478
                     /gene="ebh"
                     /locus_tag="P6L01_000170"
     CDS             5623..>37478
                     /gene="ebh"
                     /locus_tag="P6L01_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001635093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyperosmolarity resistance protein Ebh"
                     /protein_id="HDP3029272.1"
                     /translation="MNYRDKIQKFSIRKYTVGTFSTVIATLVFLGFNTSQAHAAETNQ
                     PASVVKQKQQSNNEQTENRESQVQNSQNSQNSQSLSATHENEQPNNSQANLVNQKVAQ
                     SSTTNDEQPASQNVNTKKDSATAATTQPDKEESKHKQNESQSANKNGNDNRAAHVENH
                     EANVVTASDSSDNGNVQHDRNELQAFFDANYHDYRFIDRENADSGTFNYVKGIFDKIN
                     TLLGSNDPINNKDLQLAYKELEQAVALIRTMPQRQQTSRRSNRIQTRSVESRAAEPRS
                     VSDYQNANSSYYVENANDGSGYPVGTYINASSKGAPYNLPTTPWNTLKASDSKEIALM
                     TAKQTGDGYQWVIKFNKGHAPHQNMIFWFALPADQVPVGRTDFVTVNSDGTNVQWSHG
                     AGAGANKPLQQMWEYGVNDPDRSHDFKIRNRSGQVIYSWPTVHVYSLEDLSRASDYFS
                     EAGATPATKAFGRQNFEYINGQKPAESPGVPKVYTFIGQGDASYTISFKTQGPTVNKL
                     YYAAGGRALEYNQLFMYSQLYVESTQDHQQRLNGLRQVVNRTYRIGTTKRVEVSQGNV
                     QTKKVLESTNLNIDDFVDDPLSYVKTPSNKVLGFYPTNANTNAFRPGGVQELNEYQLS
                     QLFTDQKLQEAARTRNPIRLMIGFDYPDGYGNSETLVPVNLTVLPEIQHNIKFFKNDD
                     TQNIAEKPFSKQAGHPVFYVYAGNQGNASVNLGGSVTSIQPLRINLTSNENFTDKDWQ
                     ITGIPRTLHIENSTNRTNNARERNIELVGNLLPGDYFGTIRFGRKEQLFEIRVKPHTP
                     TITTTAEQLRGTALQKVPVNISGIPLDPSALVYLVAPTNQTTNGGSEADQIPSGYTIL
                     ATGTPDGVHNTITIRPQDYVVFIPPVGKQIRAVVYYNKVVASNMSNAVTILPDDIPPT
                     INNPVGINAKYYRGDEVNFTMGVSDRHSGIKNTTITTLPSGWTSNLTKSDNKNGSLAI
                     TGRVSMNQAFNSDITFKVSATDNVNNTTNDSQSKHVSIHVGKISEDAHPIVLGNTEKV
                     VVVNPTAVSNDEKQSIITAFMNKNQNIRGYLASTDPVTVDNNGNVTLHYRDGSSTTLD
                     ATNVMTYEPVVKSEYQTANAAKTATVTIAKGQSFNIGDIKQYFTLSNGQAIPNGTFTN
                     ITSDRTIPTAQEVSQMNAGTQLYHIVASNAYHKDTEDFYISLKIVDVKQPEGDQRVYR
                     TSTYDLTTDEISKVKQAFINANRDVITLAEGDISVTNTPNGANVSTITVNINKGRLTK
                     SFASNLANMNFLRWVNFPQDYTVTWTNAKIANRPTDGGLSWSDDHKSLIYRYDATLGT
                     QITTNDILTMLKATTTVPGLRNNITGNEKAQAEAGGRPNYRTTGYSQSNATTDGQRQF
                     TLNGQVIQILDIINPSNGYGGQPVTNSNTRANHSNSTVVNVNEPAANGAGAFTIDHVV
                     KSNSTHNASDAVYKAQLYLTPYGPKQYVEHLNQNTGNTTDAINIYFVPSDLVNPTISV
                     GNYTNHQVFSGETFTNTITANDNFGVQSVTVPNTSQITGTVDNNHQHVSATAPNVTSA
                     TSKTINLLATDTSGNTATTSFNVTVKPLRDKYRVGTSSTAANPVRIANISNNATVSQA
                     DQTTIINSLTFTSNAPNRNYATASANEITSKTVSNVSRTGNNANVTVTVTHQDGTTST
                     VTVPVKHVIPEIVAHSHYTVQGQDFPAGNGSSAADYFKLSNGSAIPDATITWVSGQAP
                     NKDNTRIGEDITVTAHILIDGETTPITKTATYKVVRTVPKHVFETARGVLYPGVSDMY
                     DAKQYVKPVNNSWSTNAQHMNFQFVGTYGPNKDVVGISTRLIRVTYDNRQTEDLTILS
                     KVKPDPPRIDANSVTYKAGLTNQEIKVNNVLNNSSVKLFKADNTPLNVTNITHGSGFS
                     SVVTVSDALPNGGIKAKSSISMNNVTYTTQDEHGQVVTVTRNESVDSNDSASVTVTPQ
                     LQATTEGAVFIKGGDGFDFGHVERFIQNPPHGATVAWHDSPDTWKNTVGNTHKTAVVT
                     LPSGQGTRNVEVPVKVYPVANAKAPSRDVKGQNLTHGTNAIDYITFDPNTNTNGITAA
                     WANRQQPNNQQAGVQHLNVDVTYPGISAAKRVPVTVNVYQFEFPQTTYTTTVGGTLAS
                     GTQASGYAHMQNASGLPTDGFTYKWNRDTTGTNDANWAAMNKPNTAQVVNAKYDVIYN
                     GHTFATSLPAKFVVKDVQPAKPTVTETAAGAITIAPGANQTVNTHAGNVTTYADKLVI
                     KRNGNVVTTFTRRNNTSPWVKEASAANVTGIVGTNNGITVAAGTFNPADTIQVVATQG
                     SGETISDEQRSDDFTVVAPQPNQATTKIWQNGHIDITPNNPSGHLINPTQAMDIAYTE
                     KVGNGAEHSKTINVVRGQNNQWTIANKPDYVTLDAQTGKVTFNANTIKPNSSITITPK
                     AGTGHSVSSNPSTLTAPAAHTVNTTEIVKDYGSNVTAAEINNAVQVANKRTATIKNGT
                     AMPTNLAGGSTTTIPVTVTYNDGSTEEVQESIFTKADKRELITAKNHLDDPVSTEGKK
                     PGTITQYNNAMHNAQQQINTAKTEAQQVINNERATPQQVSDALTKVRAAQTKIDQAKA
                     LLQNKEDNSQLVTSKNNLQSSVNQVPSTAGMTQQSIDNYNAKKREAETEITAAQRVID
                     NGDATAQQISDEKHRVDNALTALNQAKHDLTADTHALEQAVQQLNRTGTTTGKKPASI
                     TAYNNSIRALQSDLTSAKNSANAIIQKPIRTVQEVQSALTNVNRVNERLTQAINQLVP
                     LADNSALRTAKTKLDEEINKSVTTDGMTQSSIQAYENAKRAGQTETTNAQNVINNGDA
                     TDQQIAAEKTKVEEKYNSLKQAIAGLTPDLAPLQTAKTQLQNDIDQPTSTTGMTSASV
                     AAFNDKLSAARTKIQEIDRVLASHPDVATIRQNVTAANAAKTALDQARNGLTVDKAPL
                     ENAKNQLQHSIDTQTSTTGMTQDSINAYNAKLTAARNKVQQINQVLAGSPTVDQINTN
                     TSAANQAKSDLDHARQALTPDKAPLQNAKTQLEQSINQPTDTTGMTTASLNAYNQKLQ
                     AARQKLTEINQVLNGNPTVQNINDKVAEANQAKDQLNTARQGLTLDRQPALTTLHGAS
                     NLNQAQQNNFTQQINAAQNHAALETIKSNITALNTAMTKLKDSVADNNTIKSGQNYTD
                     ATPANKQAYDNAVNAAKGVIGETTNPTMDVNTVNQKAASVKSTKDALDGQQNLQRAKT
                     EATNAITHASDLNQAQKNALTQQVNSAQNVQAVNDIKQTTQSLNTAMTGLKRGVANHN
                     QVVQSDNYVNADTNKKNDYNNAYNHANDIINGNAQHPVITPSDVNNALSNVTSKEHAL
                     NGEAKLNAAKQEANTALGHLNNLNNVQRQNLQSQINGAHQIDAVNTIKQNATNLNSAM
                     GNLRQAVADKDQVKRTEDYADADTAKQNAYNSAVSSAETIINQTANPTMSVDDVNRAT
                     SAVTTNKNALNGDEKLVQSKTDAARAIDALPHLNNAQKADVKSKINAASNIAGVNTVK
                     QQGTDLNTAMGNLQGAINDEQTTLNSQNYQDATPSKKTAYTNAVQAAKDILNKSNGQN
                     KTKDQVTEAMNQVNSAKNNLDGTRLLDQAKQTAKQQLNNMTHLTTAQKTNLTNQINSG
                     TTVAGVHTVQSNANTLDQAMNTLRQSIANNDATKASEDYVDANNDKQTAYNNAVAAAE
                     TIINANSNPEMNPSTITQKAEQVNSSKTALNGDENLATAKQNAKTYLNTLTSITDAQK
                     NNLISQISSATRVSGVDTVKQNAQHLDQAMANLQNGINNESQVKSSEKYRDADTNKQQ
                     EYDNAITAAKAILNKSTGPNTAQNAVEAALQRVNTAKDALNGDAKLIAAQNAAKQHLG
                     TLTHITTAQRNDLTNQISQATNLAGVESVKQSANSLDGAMGNLQTAINDKSGTLASQN
                     FLDADEQKRNAYNQAISAAETILNKQTGPNTAKTAVEQALNNVNSAKHALNGTQNLNN
                     AKQAAITAINGASDLNQKQKDALKAQANGAQRVSNANDVQRNATELNTAMGQLQHAIA
                     DKTNTLASSKYVNADSTKQNAYTTKVTNAEHIISGTPTVVTTPSEVTAAANQVNSAKQ
                     ELNGDERLRVAKQNANTAIDALTQLNTPQKAKLKEQVGQANRLEDVQSVQTNGQSLNN
                     AMKGLRDSIANETTVKASQNYTDASPNNQSTYNSAVSNAKGIINQTNNPTMDTSAITQ
                     ATTQVNNAKNGLNGAENLRNAQNTAKQNLNTLSHLTNNQKSAISSQIDRAGHVSEVTA
                     AKNAATELNAQMGNLEQAIHDQNTVKQGVNFTDADKAKRDAYTNAVSRAETILNKTQG
                     ANTSKQDVEAAIQNVTSAKNALNGDQNVTNAKNAAKNALNNLTSINNAQKRDLTTKID
                     QATTVAGVEAVSNTGTQLNTAMANLQNGINDKANTLASENYHDADSDKKTAYTQAVTN
                     AENILNKNSGSNLDKAAVENALSQVTNAKGALNGNHNLEQAKSNANTTINGLQHLTTA
                     QKDKLKQQVQQAQNVAGVDTVKSSANTLNGAMGTLRNSIQDNTATKNGQNYLDATERN
                     KTNYNNAVDSANGVINATSNPNMDANAINQIATQVTSTKNALDGTHNLTQAKQTATNA
                     IDGATNLNKAQKDALKAQVTSAQRVANVTSIQQTANELNTAMGQLQHGIDDENATKQT
                     QKYRDAEQSKKTAYDQAVAAAKAILNKQTGSNSDKAAVDRALQQVTSTKDALNGDAKL
                     AEAKAAARQNLGTLNHITNAQRTALEGQINQATTVDGVNTVKTNANTLDGAMNSLQGA
                     INDKDATLRNQNYLDADESKRNAYTQAVTAAEGILNKQTGGNTSKADVDNALNAVTRA
                     KAALNGAENLRNAKTSATNTINGLPNLTQLQKDNLKHQVEQAQNVVGVNGVKDKGNTL
                     NTAMGALRTSIQNDNTTKTSQNYLDASDSNKNNYNTAVNNANGVINATNNPNMDANAI
                     NDMANQVNTTKAALNGAQNLAQAKTNATNTINNAQDLNQKQKDALKTQVNNAQRVSDA
                     NNVQHTATELNGAMTVLKAAIADKERTKASGNYVNADQEKRQAYDSKVTNAENIINGT
                     PNATLTVNDVNSAASQVNAAKTALNGDNNLRVAKEHANNTIDGLAQLNNVQKAKLKEQ
                     VQSATTLDGVQTVKNSSQTLNTAMKGLRDSIANEATIKAGQNYTDASPNNRNEYDSAV
                     TAAKAIINQTSNPTMEPNTITQATSQVTTKEHALNGAQNLAQAKTTAKNNLNNLTSIN
                     NAQKDALTRNIDGATTVAGVNQETAKATELNNAMHSLQNGINDETQTKQTQKYLDAEP
                     SKKSAYDQAVNAAKAILTKASGQNVDKAAVEQALQNVNSTKTALNGDAKLNEAKAAAK
                     QTLGTLTHINNAQRNALDNEITQATNVEGVNTVKAKAQQLDGAMGQLETSIRDKDTTL
                     QSQNYQDADDAKRTAYSQAVNAAATILNKTAGGNTPKADVERAMQAVTQANTALNGIQ
                     NLERAKQAANTAITNASDLNTKQKEALKAQVTSAGRVSAANGVEHTATELNTAMTALK
                     RAIADKADTKASGNYVNADANKRQAYDEKVTAAEHIVSGTPTPTLTPSDVTNAATQVT
                     NAKTQLNGNHNLEVAKQNANTAIDGLTSLNGPQKAKLKEQVGQATTLPNVQTVRDNAQ
                     TLNTAMKGLRDSIANEATIKAGQNYTDASQNKQNDYNNAVTAAKAIIGQTTSPSMIAQ
                     EINQAKDQVTAKQQALNGQENLRTAQTNAKQHLNGLSDLTNAQKDAAKRQIEGATHVN
                     EVTQAQNNADALNTAMTNLKNGIQDQNTIKQGVNFTDADEAKRNAYTNAVTQAEQILN
                     KAQGPNTAKDGVETALQNVQRAKNELNGNQNVANAKTTAKNALNNLTSINNAQKAALK
                     SQIEGATTVAGVNQVSTMASELNTAMSNLQRGINDEAATKAAQKYTEADRDKQTAYND
                     AVTAAKTLLDKTAGSNDNKVAVEQALQRVNTAKTALNGDARLNEAKNTAKQQLATMSH
                     LTNAQKANLTEQIERGTTVAGVQGIQANAGTLNQAMNQLRQSIASKDATKSSEDYQDA
                     NADLQNAYNDAVTNAEGIISATNNPEMNPDTINQKASQVNSAKSALNGDEKLAAAKQT
                     AKSDIGRLTDLNNAQRTAANAEVDQAPNLAAVTAAKNKATSLNTAMGNLKHALAEKDN
                     TKRSVNYTDADQPKQQAYDTAVTQAEAITNANGSNANETQVQAALNQLNQAKNDLNGD
                     NKVAQAKETAKRALASYSNLNNAQSTAATSQIDNATTVADVTAAQNTANELNTAMGQL
                     QNGINDQNTVKQQVNFTDADQGKKDAYTNAVTNAQGILDKANGQNMTKAQVEAALNQV
                     TTAKNALNGDANVRQAKSDAKANLGTLTHLNNAQKQDLTSQIEGATTVNGVNSVKTKA
                     QDLDGAMQRLESAIANKDQTKASENYIDADPTKKTAFDNAITQAESYLNKDHGTNKDK
                     QAVEQAIQSVTSTENALNGDANLQRAKTEATQAIDNLTQLNTPQKTALKQQVNAAQRV
                     SGVTDLKNSATSLNNAMDQLKQAIGDHDTIVAGGNYTNASPDKQGAYTDAYNAAKNIV
                     NGSPNVITNAADVTAATQRVNNAETSLNGDTNLATAKQQAKDALRQMTHLSDAQKQSI
                     TGQIDSATQVTGVQSVKDNATNLDNAMNQLRNSIANKDEVKASQPYVDADTDKQNAYN
                     TAVTSAENIINATSQPTLDPSAVTQAANQVNTNKTALNGAQNLANKKQETTANINRLS
                     HLNNAQKQDLNTQVTNAPNISTVNQVKTKAEQLDQAMERLINGIQDKDQVKQSVNFTD
                     ADPEKQTAYNNAVTAAENIINQANGTNANQSQVEAALSTVTTTKQALNGDRKVTDAKN
                     NANQTLSTLDNLNNAQKGAVTGNINQAHTVAEVTQAIQTAQELNTAMGNLKNSLNDKD
                     TTLGSQNFADADPEKKNAYNEAVRNAENILNKSTGTNVPKDQVEAAMNQVNTTKAALN
                     GTQNLEKAKQHANTAIDGLSHLTNAQKEALKQLVQQSTTVAEAQGNEQKANNVDAAMD
                     KLRQSIADNATTKQNQNYTDASPNKKDAYNNAVTTAQGIIDQTTNPSLDPTVINQAAG
                     QVSTSKNALNGNENLEAAKQQATQSLGSLDNLNNAQKQAVTNQINGAHTVDEANQIKQ
                     NAQNLNTAMGNLKQAIADKDATKATVNFTDADQAKQQAYNTAVTNAENIISKANGGNA
                     TQTEVEQAIQQVNAAKQALNGNANVQHAKDEATALINNSNDLNQAQKDALKQQVQNAT
                     TVAGVNNVKQTAQELNNAMTQLKQGIADKEQTKADGNFVNADSDKQNAYNQAVAKAEA
                     LISGTPDVVVTPSEITAALNKVTQAKNDLNGNTNLATAKQNVQHAIDQLPNLNQAQRD
                     EYSKQITQATLVPNVNAIQQAATTLNDAMTQLKQGIANKAQIKGSENYHDADTDKQTA
                     YDNAVTKAEELLKQTTNPTMDPNTIQQALTKVNDTNQALNGNQKLADAKQDAKTTLGT
                     LDHLNDAQKQALTTQVEQAPDIATVNNVKQNAQNLNNAMTNLNNALQDKTETLNSINF
                     TDADQAKKDDYTNAVSHAEGILSKANGSNASQTEVEQAMQRVNEAKQALNGNDNVQRA
                     KDAAKQVITNANDLNQAQKDALKQQVDAAQTVANVNTIKQTAQDLNQAMTQLKQGIAD
                     KDQTKANGNFVNADTDKQNAYNNAVAHAEQIISGTPNANVDPQQVAQALQQVNQAKGD
                     LNGNHNLQVAKDNANTAIDQLPNLNQPQKTALKDQVSHAELVTGVNAIKQNADALNNA
                     MGTLKQQIQANSQVPQSVDFTQADQDKQQAYNNAANQAQQIANGTPTPVLAPDTVTKA
                     VTTMNQAKDALNGDEKLAQAKQDALANLDTLRDLNQPQRDALRNQINQAQALATVEQT
                     KQNAQNVNTAMGNLKQGIANKDTVKASENYHDADVDKQTAYTNAVSQAEGIINQTTNP
                     TLNPDDITRALTQVTDAKNSLNGEAKLATEKQNAKDAVSGMTHLNDAQKQALKGQIDQ
                     SPEIATVNQVKQTATSLDQAMDQLSQAINDKDQILADGNYLNADPDKQNAYKQAVAKA
                     EALLNKQSGTNEVQAQVESITNEVNAAKQALNGNDNLANAKQQAKQQLANLTHLNDAQ
                     KQSFESQITQAPLVTDVTTINQKAQTLDHAMELLRNSVADNQTTLASEDYHDATAQRQ
                     NDYNKAVTAANNIINQTTSPTMNPDDVNGATTQVNNTKVALDGDENLAAAKQQANNRL
                     DQLDHLNNAQKQQLQSQITQSSDIAAVNGHKQTAESLNTAMGNLINAIADHQAVEQRG
                     NFINADTDKQTAYNTAVNEAAAMINKQTGQNANQTEVEQAITKVQTTLQALNGDHNLQ
                     VAKTNATQAIDALTSLNDPQKTALKDQVTAATLVTAVHQIEQNANTLNQAMHGLRQSI
                     QDNAATKANSKYINEDQPEQQNYDQAVQAANNIINEQTATLDNNAINQVAATVNTTKA
                     ALHGDVKLQNDKDHAKQTVSQLAHLNNAQKHMEDTLIDSETTRTAVKQDLTEVQALDQ
                     LMDALQQSIADKDATRASSAYVNAEPNKKQAYDEAVQNAESIIAGLNNPTINKGNVSS
                     ATQAVISSKNALDGVERLAQDKQTAGNSLNHLDQLTPAQQQALENQINNATTRDKVAE
                     IIAQAQALNEAMKALKESIKDQPQTEASSKFINEDQAQKDAYTQAVQHAKDLINKTTD
                     PTLAKSIIDQATQAVTDAKNNLHGDQKLAQDKQRATETLNNLSNLNTPQRQALENQIN
                     NAATRGEVAQKLTEAQALNQAMEALRNSIQDQQQTESGSKFINEDKPQKDAYQAAVQN
                     AKDLINQTGNPTLDKAQVEQLTHAFKQAKDNLHGDQKLADDKQHAVTDLNQLNGLNNP
                     QRQALESQINNAATRGEVAQKLAEAKALDQAMQALRNSIQDQQQTEAGSKFINEDKPQ
                     KDAYQAAVQNAKDLINQTGNPTLDKSQVEQLTQAVTTAKDNLHGDQKLARDQQQAVTT
                     VNALPNLNHAQQQTLTDAINAAPTRTEVAQHVQTATELDHAMETLKNKVDQVNTDKAQ
                     PNYTEASTDKKEAVDQALQAAQSITDPTNGSNANKDAVEQALTKLQEKVNELNGNERV
                     AEAKTQAKQTIDQLTHLNADQIATAKQNIDQATKLQPIAELVDQATQLNQSMDQLQQA
                     VNEHANVEQTIDYTQADSDKQKAYKQAIADAENVLKQNANKQQVDQALQNILNAKQAL
                     NGDERVALAKTNGKHDIDQLNALNNAQQDGFKGRIDQSNDLNQIQQIVDEAKALNRAM
                     DQLSQEITGNEGRTKGSTNYVNADTQVKQVYDEAIDKAKQALDKSSGQNLTAEQVIKL
                     NDAVTAAKKALNGEERLNNRKAEALQRLDQLTHLNNAQRQLAIQQINNAETLNKASRA
                     INRATKLDNAMGAVQQYIDEQHLGVISSTNYINADDNLKANYDNAIANAAHELDKVQG
                     NAIAKAEAEQLKQNIIDAQNALNGDQNLANAKDKANAFVNSLNGLNQQQQDLAHKAIN
                     NADTVSDVTDIVNNQIDLNDAMETLKHLVDNEIPNAEQTVNYQNADDNAKTNFDDAKR
                     LANTLLNSDNTNVNDINGAIQAVNDAIHNLNGDQRLQDAKDKAIQSINQALANKLKEI
                     EASNATDQDKLIAKNKAEELANSIINNINKATSNQAVSQVQTAGNHAIEQVHANEIPK
                     AKIDANKDVDKQVQALIDEIDRNPNLTDKEKQALKDRINQILQQGHNDINNALTKEEI
                     EQAKAQLAQALQDIKDLVKAKEDAKQDVDKQVQALIDEIDQNPNLTDKEKQALKDRIN
                     QILQQGHNGINNAMTKEEIEQAKAQLAQALKEIKDLVKAKENAKQDVDKQVQALIDEI
                     DQNPNLTDKEKQALKDRINQILQQGHNDINNAMTKEEIEQAKAQLAQALQDIKDLVKA
                     KEDAKNAIKALANAKRDQINSNPDLTPEQKAKALKEIDEAEKRALQNVENAQTIDQLN
                     RGLNLGLDDIRNTHVWEVDEQPAVNEIFEATPEQILVNGELIVHRDDIITEQDILAHI
                     NLIDQLSAEVIDTPSTATISDSLTAKVEVTLLDGSKVIVNVPVKVVEKELSVVKQQAI
                     ESIENAAQQKIDEINNSVTLTLEQKEAAIAEVNKLKQQAIDHVNNAPDVHSVEEIQQQ
                     EQAYIEQFNPEQFTIEQAKSNAIKSIEDAIQHMIDEIKARTDLTDKEKQEAIAKLNQL
                     KEQAIQAIQRAQSISEITEQLEQFKAQMKAANPTAKELAKRKQEAISRIKDFSNEKIN
                     SIRNSEIGTADEKQAAMNQINEIVLETIRDINNAHTLQQVEAALNNGIARISAVQIVI
                     SDRAKQSSSTGNESNSHLTIGYGTANHPFNSSTIGHKKKLDEDDDIDPLHMRHFSNNF
                     GNVIKNAIGVVGISGLLASFWFFIAKRRRKEDEEEELEIRDNNKDSIKETLDDTKHLP
                     LLFAKRRRKEDEEDVTVEEKDSLNNGESLDKVKHTPFFLPKRRRKEDEEDVEVTNENT
                     DEKVLKDNEHSPLLFAKRRKDKEEDVETTTSIESKDEDVPLLLAKKKNQKDNQSKDKK
                     SASKNTSKKVAAKKKKKKS"
BASE COUNT        14963 a         6519 c         6792 g         9204 t
ORIGIN      
        1 tatttacata ttcataacca aaggataaaa taattgtgaa attattcaca aatgaaagag
       61 gagtgttgca aatgttagtt gcagtagtca aagaattaaa acaaggtgaa ggacgtgtag
      121 cttgcacacc cgaaaatgtg cgtaagttaa cggatgcagg acataaagta attgttgaaa
      181 aaaatgctgg cattggttca ggattttcta acgatatgta tgaaaaagaa ggcgctaaga
      241 tcgtaactca cgaacaagca tgggaagctg atcttgttat caaagtaaaa gaacctcatg
      301 aaagcgaata tcaatatttc aaaaagaatc aaattatctg gggattttta catctagcat
      361 cttcaaaaga aatagtagaa aaaatgcaag aagttggtgt aactgcgatt agtggtgaaa
      421 ccattataaa aaatggaaaa gcagaattat tagcgccaat gagtgctata gcaggtcaac
      481 gctcagcaat tatgggagct tactactctg aagcacaaca tggtggtcaa ggtactttag
      541 tgactggtgt acatgaaaat gtggatatac ctggtagtac atatgtgatt ttcggtggtg
      601 gcgtagcagc aacaaatgca gcaaatgttg ccttgggact aaatgctaaa gtaatcatta
      661 tcgagttaaa cgatgaccgc attaaatatc ttgaagatat gtatgcagaa aaagatgtca
      721 cagtagtcaa atcaacacca gaaaatttag cagaacaaat taagaaagca gatgtattta
      781 tttctacaat tttaattcca ggtgcgaaac cgccaaaatt ggttactcgt gagatggtta
      841 aatcaatgaa aaaaggttca gtattaatcg atatagctat tgaccaaggt ggaactattg
      901 aaacaattag accaactaca atttctgatc cagtgtatga agaagaaggt gtgattcatt
      961 atggtgtacc aaatcaacca ggagcagtcc caagaacttc aacaatggca ttagcacaag
     1021 gaaatattga ttatatatta gaaatttgtg acaaaggctt agaacaagca attaaagata
     1081 atgaagcctt aagtactggt gtaaacattt accaaggaca agtgacaaat caaggattag
     1141 cttcatcaca tgacctagat tataaagaaa tattaaatgt tatcgaatag ataataattt
     1201 aaatgaaatt gagtgaaatg aatattttaa atatagcatt atagtttgga ctaaaaattt
     1261 acaaaacgga aggatgtaat tattatgaca accaacacag ttacattaca aacagcacat
     1321 attgtaagtt taggagatat cgaagaagct aaagcaagca ttaaaccatt tattcgtcga
     1381 acaccactaa ttaaatctat gtatttaagt caaaatatca ctaaaggtaa cgtatattta
     1441 aaacttgaaa atatgcagtt tacaggttca tttaaattta gaggtgctag caataaaatt
     1501 aatcatttgt cagatgaaca aaaagctaaa ggtattattg gcgcatcagc cggaaatcat
     1561 gcacaaggtg tagctttgac agcaaaacta ttaggtattg atgcaacgat tgtgatgcca
     1621 gaaacagcac caattgctaa acagaatgct accaaaggat atggtgcaaa agtcatttta
     1681 aaaggtaaaa actttaatga aactagactt tatatggaag aattagcgaa agaaaatggt
     1741 atgacaattg ttcatccata tgatgataag tttgtcatgg ctggtcaagg aacaatagga
     1801 ttagaaattt tagatgatat atggaatgtt aatacggtca ttgttccagt tggtggaggt
     1861 ggcctaattg caggtattgc caccgcttta aaatcattca acccatcaat tcatataatc
     1921 ggcgttcaag ctgaaaatgt gcatggcatg gcagagtcat tctataagag agctttaact
     1981 gaacatcgtg aagatagcac aattgcagat ggttgtgatg taaaagtacc aggtgaaaaa
     2041 acatacgaag tagtaaaaca tttagtagat gaatttattc ttgtatcaga agaggaaata
     2101 gaacatgcaa tgcaagattt aatgcaacgt gccaaaatca ttacagaagg tgcaggagct
     2161 ttacctacag cagctatttt aagtgggaaa attgataaaa aatggctaga gggtaaaaat
     2221 gtcgttgcat tagtttcagg aggaaacgtt gacttaacta gagtttctgg tgtcattgaa
     2281 catggcttaa atatcgcgga tacaagtaag ggtgtggtag gttaaaactt ccaatcttaa
     2341 aaatgaggtg taattatgtc aaatggtaaa gaattacaaa aaaatatagg tttcttctca
     2401 gcgtttgcta ttgttatggg gacagttatt ggttcaggag tattctttaa aatatcaaac
     2461 gtaacagaag taacaggaac agcaggaatg gccttgtttg tatggttcct aggcggcatc
     2521 attaccattt gtgcggggtt aacagcagca gaacttgctg ctgcaatccc tgaaacaggt
     2581 ggcttaacga agtatataga atatacatac ggtgatttct ggggcttcct atcaggttgg
     2641 gcgcaatcat ttatttattt tccagctaac gtagcagcat tgtctatcgt atttgcgaca
     2701 cagctaatta atttattcca tttatctata ggttcgttaa taccaatagc aatcgcatct
     2761 gcgttatcta ttgtgttgat aaatttccta ggttcaaaag caggcggaat tttacaatca
     2821 gttactttag taattaaact gattccaatc atcgttattg taatttttgg tatttttcaa
     2881 tctggagata tcactttttc attaattcca attacaggta attcaggaaa tggtttcttt
     2941 acagcaattg gtagtggttt attagcaact atgtttgcat atgatggttg gattcatgta
     3001 ggaaatgttg cgggggaact taaaaatcct aaacgcgatt tacctttagc gatttcagtt
     3061 ggtatcggtt gtattatggc tgtgtattta ttaattaacg ctacattttt attaacgttg
     3121 ccaatagaac tacttgctgg taacttaaat gcagcttcag atacatcaaa aatattattt
     3181 ggtgaaaatg gcggtaaaat tattacaatc ggtatattaa tttctgttta tggtacgatc
     3241 aatggctata ctatgactgg tatgcgcgta ccatatgcaa tggctgaaag aaaattattg
     3301 ccatttagcc acttattcgc aaaattaaca aaatctggcg caccatggtt tggcgcaatt
     3361 atacaactta taatcgctat catcatgatg tcaatgggag catttgatac aataacaaac
     3421 atgttaatct ttgttatttg gttgttctat tgtatgtcat ttgttgctgt aataatttta
     3481 agaaaacgtg aaccaaatat ggagcgtcca tataaagtac cgttatatcc gatcatacct
     3541 ttaattgcta ttttggcagg atcatttgta ttgattaata cactgtttac acaatttata
     3601 ttagcaatca ttggaattct aataacagca cttggtatac cagtttatta ctataagaag
     3661 aaacaaaaag cagcataagg taagataact agcattgaga ataaatggat ggactactaa
     3721 taaatttaaa gttttacaca ttaaaatcaa aaaccattca attattctat cgaacagaca
     3781 aatttctgtt atggaatttg tctgttttca aaagtatagg gaggcaaata gagatggaaa
     3841 agccgtcaag agaggcattt gaaggcaata ataagttgtt aataggaatt gttttaagtg
     3901 taataacgtt ttggctattt gcacaatcat tggttaatgt tgtaccaata cttgaagata
     3961 gtttcaatac agatattgga acggttaata tcgccgttag tataactgct ttattttcag
     4021 gaatgtttgt tgtaggagca ggtggtcttg ctgataaata tggcagaatt aaactcacga
     4081 acattggtat tatcttaaat atattgggtt cattattaat cattatttca aatattcctt
     4141 tattacttat tataggaaga ttaattcaag gactttcagc agcatgtatt atgcctgcaa
     4201 ctttgtctat tattaagtca tattacattg ggaaagatag acaacgcgct ttaagttatt
     4261 ggtcaattgg ctcatggggc ggctctggtg tttgttcatt ttttggaggt gcagttgcaa
     4321 cacttttagg ttggcgttgg attttcatcc tatcaattat aatttcatta attgcgctgt
     4381 ttcttattaa aggcacacct gaaactaaat ctaaatcgat ttctctaaat aaatttgaca
     4441 ttaaaggtct ggttctttta gtcattatgc tcctcacttt aaatatttta attactaaag
     4501 gatcagaatt aggtgtaacc tcacttcttt ttatcacttt attagcaatt gcaattggat
     4561 cttttagttt atttatagtt cttgaaaagc gtgctacaaa tcctttaatc gattttaaat
     4621 tatttaaaaa taaagcttat acaggtgcaa cagcttcaaa ctttttgtta aatggtgttg
     4681 cgggaacatt aatagtagcc aacacatttg ttcaaagagg tttaggatat tcctcattgc
     4741 aagcaggaag tttatcaatc acttatttag taatggtact aattatgatt cgtgttggtg
     4801 aaaagttact tcaaacactc ggatgcaaga aaccaatgtt aattggaaca ggagttctta
     4861 ttgtcggaga atgtctcatt tcattaactt tcttgccaga aatattgtat gtcatttgtt
     4921 gtattatagg ttatttattc tttggtttag gactaggaat atatgctaca ccatcaacag
     4981 atacagcaat tgcaaatgca ccgttagaaa aagtaggagt tgctgcaggt atctataaaa
     5041 tggcttctgc attaggtgga gcatttggcg tcgcattgag tggtgcagtt tatgcaatcg
     5101 tatcaaatat gacaaacatt tatactggtg caatgattgc attatggtta aacgcaggta
     5161 tgggaatatt atcattcgtt atcattttgt tacttgtgcc taaacaaaac gacactcaat
     5221 tatgataatt gagaattaaa ttgaaatcat acaagtcgct acaatattaa acaaaaatat
     5281 aaaccgattc ttatgtgtca ttattttaaa tgaacatagg gattggtttt ttattactct
     5341 tttacgctac tttatttata attattataa attgtcacaa attcaattta ccttacaata
     5401 tattttgtgt tattatattc tggagcataa ataaattgtt caacacatag ttgtaatgtg
     5461 tttcaatact ttttggatag attgcgaaat tgtattgaat cgtcatcgtt ttaaattttt
     5521 aaatgagaat ggaatgagca ttacaatata caagcaatca aaagtaaata cattcacaac
     5581 acaacagaga cataacaaca agataaggag tgaacaatag ctgtgaatta tcgtgataaa
     5641 attcaaaagt ttagtattcg taaatataca gttggtacat tttcaactgt cattgcgaca
     5701 ttggtatttt taggattcaa tacatcacaa gcacatgctg ctgaaacaaa tcaaccagca
     5761 agcgtggtta aacagaaaca acaaagtaat aatgaacaga ctgagaatcg agaatctcaa
     5821 gtacaaaatt ctcaaaattc acaaaatagt caatcattat ccgctactca tgaaaatgag
     5881 caaccaaata atagtcaagc taatttagta aatcaaaaag tagcgcaatc atctactact
     5941 aatgatgaac aaccagcatc tcaaaatgta aatacaaaga aagattcggc aacggctgcg
     6001 acaacacaac cagataaaga agaaagtaag cataaacaaa acgaaagtca atctgctaat
     6061 aaaaatggaa acgacaatag agcggctcat gtagaaaatc atgaagcaaa tgtagtaaca
     6121 gcttcagatt catctgataa tggtaacgta caacatgacc gaaatgaatt acaagcattt
     6181 tttgatgcaa attatcatga ttatcgcttt attgaccgtg aaaatgcaga ttctggcaca
     6241 tttaactatg taaaaggcat ttttgacaag attaatactt tattaggcag taatgatcca
     6301 attaacaata aagacttgca acttgcatac aaagaattgg aacaagctgt tgctttaatt
     6361 cgtacaatgc ctcaacgtca acaaactagc cgtcgatcaa acagaattca aacgcgttct
     6421 gttgagtcta gagctgcaga gcctagatca gtatcagact atcaaaatgc aaattcatca
     6481 tattatgttg aaaatgctaa tgatggttca ggatatcctg taggtacata tatcaatgct
     6541 tctagtaaag gggcgccata taatttacca actacaccat ggaatacatt gaaggcctct
     6601 gactcaaagg aaattgctct tatgacagcg aaacaaactg gagatggcta ccaatgggtt
     6661 attaagttta ataaaggaca tgctccacat caaaatatga ttttctggtt tgcattacca
     6721 gcagaccaag tgccagtagg aagaactgac tttgtaacag ttaattcaga tggaacaaat
     6781 gtacaatgga gtcatggagc aggagcaggt gcaaataaac cacttcaaca aatgtgggaa
     6841 tatggagtaa atgatcctga tcgttcacat gactttaaaa taagaaatag aagtggccaa
     6901 gtaatatata gctggccaac tgtccatgtt tattctttag aagatttatc tagagcgagt
     6961 gattatttta gtgaagctgg agcgacacct gctactaaag catttggtag acaaaatttt
     7021 gaatatatta atggtcaaaa acctgctgaa tcaccgggtg ttcctaaagt ttatactttc
     7081 atcggtcaag gtgatgcaag ttatacaatt tcatttaaaa cacaaggtcc aactgttaat
     7141 aaattgtatt atgcagcagg tgggcgtgct ttagagtaca atcaattatt tatgtacagt
     7201 caactatacg tcgaatcaac gcaagaccat caacaacgtc ttaatggttt aagacaagtg
     7261 gttaatcgta catatcgcat aggtacaact aaacgtgtag aagtgagtca aggaaatgta
     7321 caaacgaaaa aggtattaga aagtacaaac ctaaatatag atgattttgt tgatgatcct
     7381 ttaagttatg ttaagacgcc gagtaataaa gtgttaggtt tttacccaac taatgcaaat
     7441 actaacgctt ttagaccggg gggcgttcaa gaattaaatg aatatcaatt aagtcaatta
     7501 tttactgatc aaaaattaca agaagcagca agaactagaa acccaataag attaatgatt
     7561 ggtttcgact atcctgatgg ttatggtaat agtgaaactt tagttcctgt taacttaacg
     7621 gtattacctg aaatccaaca taatattaaa ttctttaaaa atgacgatac tcaaaatatt
     7681 gctgaaaaac cattttcaaa acaagctggg catccagttt tctatgtata tgcaggtaac
     7741 caagggaatg cttccgtgaa tttaggtggt agcgtaacat ctattcaacc attacgtatt
     7801 aatttaacaa gtaatgagaa ttttacagat aaagattggc aaattacagg tattccgcgt
     7861 acattacaca ttgaaaactc gacaaataga actaataatg ctagagaacg taacattgaa
     7921 cttgttggta atttattacc aggggattac tttggtacga tacgttttgg acgtaaagaa
     7981 caattatttg aaattcgtgt taaaccacat acaccaacaa ttacaacgac agctgagcaa
     8041 ttaagaggta cagcattaca aaaagtgcct gttaatattt cgggaatacc gttggatcca
     8101 tcggcattgg tttatttagt tgcaccaaca aatcaaacta cgaatggtgg tagtgaggca
     8161 gatcaaatac catctggtta tacgatactt gcgactggta cacctgatgg ggtgcataat
     8221 acaattacta tacgaccgca agattatgtt gtattcatac cacctgtagg taaacaaatt
     8281 agagcagtag tttattataa taaagtagtt gcatctaata tgagtaatgc tgttactatt
     8341 ttgccagatg acattccacc aacaatcaat aatcctgttg gaataaatgc caaatactat
     8401 cgaggcgacg aagtcaactt tacaatggga gtctctgata gacattctgg tataaaaaat
     8461 acaactatta ctactttgcc aagtggttgg acatcaaatt taactaaatc cgacaacaaa
     8521 aacggctcat tagctattac aggtagagtc tctatgaatc aggcatttaa cagtgatatt
     8581 acatttaaag tatcagcgac agacaatgtc aataatacga caaatgatag tcaatctaaa
     8641 catgtgtcaa ttcatgtagg taaaattagt gaagatgctc atccgattgt attaggaaat
     8701 actgagaaag ttgtagtagt caatccgact gctgtatcta atgatgaaaa gcaaagcata
     8761 attactgcct ttatgaataa aaaccaaaat ataagaggat atttagcatc aactgatcca
     8821 gtaactgtcg ataataatgg taacgtcaca ttacattacc gtgatggctc atcaacaacg
     8881 cttgatgcta caaatgtgat gacatacgaa ccagttgtga aatctgaata tcaaactgcc
     8941 aatgctgcta aaacagcaac ggtaacgatt gctaaaggac aatcatttaa tattggtgat
     9001 attaaacaat attttacttt aagtaatgga caagctattc caaatggcac atttacaaat
     9061 attacatctg atagaactat tccaactgca caagaagtta gtcaaatgaa tgcaggtacg
     9121 cagttatatc atatagttgc ttcaaatgca tatcataaag acactgaaga tttctatatt
     9181 agtttaaaaa tcgttgatgt gaaacaacct gaaggcgatc aacgtgtcta tcgtacgtca
     9241 acatatgatt taaccactga tgaaatctca aaagtaaaac aagcttttat taatgcaaat
     9301 agagatgtaa ttacgcttgc cgaaggtgat atttcagtta caaatacacc taatggtgct
     9361 aatgtaagta ctattacagt aaatattaat aaaggtcgat taacgaaatc attcgcgtct
     9421 aacctagcta atatgaattt cttgcgttgg gttaatttcc cacaagatta tacagtgaca
     9481 tggacgaatg caaaaattgc aaacagacca acagatggtg gtttatcatg gtccgatgac
     9541 cataaatctt taatttatcg ttatgatgct acattaggca cacaaattac aactaatgat
     9601 attttaacga tgctaaaagc gactactaca gtgcctggat tgcgtaataa tattactggt
     9661 aatgaaaaag cacaagcaga agcaggtgga agaccaaact atagaacaac tggttattca
     9721 caatcaaatg cgacaactga tggtcaacgt caatttacgt tgaatggtca agtgattcaa
     9781 atattagaca tcatcaaccc ttcaaacggt tatggtgggc aacctgttac aaattcaaat
     9841 actcgtgcaa accatagtaa ctcaactgtt gttaacgtaa acgaaccggc agctaatggt
     9901 gctggcgcat ttacaattga ccacgttgta aaaagtaatt ctacacataa tgcaagtgat
     9961 gcagtttata aagcgcagtt atacttaacg ccatatggtc caaaacaata tgttgaacat
    10021 ttaaatcaaa atacaggaaa tactactgac gctattaaca tttattttgt accaagtgac
    10081 ttagtgaatc caacaatttc agtaggtaat tacactaatc atcaagtgtt ctcaggtgaa
    10141 acatttacaa atacgattac agcgaatgat aactttggtg tgcaatcggt aactgtacca
    10201 aatacatcac aaattacagg tactgttgat aataaccatc aacatgtttc tgcaacggca
    10261 ccaaatgtga catcagcaac tagtaagaca atcaatttat tagcaactga tacaagtggt
    10321 aatacagcta caacttcatt caatgtaaca gtgaaacctt tgcgtgataa atatcgagtt
    10381 ggtacttcat caacggctgc taatcctgtt agaattgcca atatttcgaa taatgcgaca
    10441 gtatcacaag ctgatcaaac gacaattatt aattcgttaa cgtttacaag taatgcacca
    10501 aatagaaact atgcaacagc aagcgcaaat gaaatcacta gtaaaacagt tagtaatgtc
    10561 agtcgtactg gaaataatgc caatgtcaca gtaactgtta ctcatcaaga tggaacaaca
    10621 tcaacagtga ctgtacctgt aaagcatgtc attccagaaa tcgttgcaca ttcgcattac
    10681 actgtacaag gccaagactt cccagcaggt aatggttcta gtgcagcaga ttactttaag
    10741 ttatctaatg gtagtgccat tccagatgca acgattacat gggtaagtgg acaagcgcca
    10801 aataaagata atacacgtat tggtgaagat ataacagtaa ctgcacatat cttaattgat
    10861 ggcgaaacaa cgccgattac gaaaacagca acatataaag tagtaagaac tgtaccgaaa
    10921 catgtctttg aaacagccag aggtgtttta tacccaggtg tttcagatat gtatgatgcg
    10981 aaacaatatg ttaagccagt aaataattct tggtcgacaa atgcgcaaca tatgaatttt
    11041 caatttgttg gaacatatgg tcctaacaaa gatgttgtag gtatatcaac gcgtcttatt
    11101 agagtgactt atgataatag acaaactgaa gatttaacta ttttatctaa agttaaacct
    11161 gacccaccaa gaattgacgc aaactctgtg acatataaag caggtcttac aaaccaagaa
    11221 attaaagtta ataacgtatt aaataactcg tcagtaaaat tatttaaagc agataataca
    11281 ccattaaatg tcacaaatat tactcatggt agtggtttta gttcggttgt gacagtaagt
    11341 gacgcgttac caaatggcgg aattaaagca aaatcttcaa tttcaatgaa caatgtgacg
    11401 tatacgacgc aagacgaaca tggtcaagtt gttacagtaa caagaaatga atctgttgat
    11461 tcaaatgata gtgcttctgt tacagtaaca ccacaattac aagcaactac tgaaggcgct
    11521 gtatttatta aaggtggcga cggttttgat ttcggtcatg tagaacgatt tattcaaaat
    11581 ccgccacatg gggcaacggt cgcatggcat gatagtccag atacatggaa gaatacagtc
    11641 ggcaacacac ataaaactgc ggttgtaaca ttacctagtg gtcaaggtac gcgtaatgtt
    11701 gaagttccag tcaaagttta tccagttgct aatgctaagg cgccatcacg tgatgtgaaa
    11761 ggtcaaaatt tgacacatgg tacaaacgct attgattaca ttacatttga tccaaatact
    11821 aatacgaatg gtattacagc agcatgggca aatagacaac aaccaaataa ccagcaagca
    11881 ggcgttcaac atttaaatgt cgatgtcaca tatccaggta tttcagctgc taaacgagtt
    11941 cctgtaactg tgaacgtata tcaatttgaa ttccctcaaa ctacttatac aacaacagtt
    12001 ggtggcactt tagcaagtgg tacgcaagca tcaggatatg cacatatgca aaacgcttca
    12061 ggtttaccaa cagatggatt tacgtataaa tggaatcgtg atactacggg tacaaacgat
    12121 gcaaactggg cagcaatgaa taaaccaaat actgcacaag tcgttaatgc aaaatatgat
    12181 gtcatctata atggacatac atttgcaaca tctttaccag cgaaatttgt agtaaaagat
    12241 gttcaaccag cgaaaccaac tgtcactgaa acagcggcag gagcgattac aattgcacct
    12301 ggtgcgaacc aaacagtcaa tactcatgct ggtaatgtta cgacatatgc tgacaaatta
    12361 gttattaaac gtaatggaaa tgttgtaacg acatttacac gtcgtaataa tacgagccca
    12421 tgggtgaaag aagcatcagc agctaatgta acaggtattg ttggaactaa taatggtatt
    12481 actgtggcag caggtacttt caatcctgct gatacaattc aagttgttgc aacacaaggt
    12541 agtggcgaaa caatcagtga cgagcaacgt agtgatgatt tcacagttgt cgcaccacaa
    12601 ccgaaccaag cgactacgaa aatttggcaa aatggtcata ttgatatcac gcctaataat
    12661 ccatcaggac atttaattaa tccaacacaa gcaatggata ttgcttacac tgaaaaagtg
    12721 ggtaatggtg cagaacatag taagacaatt aatgttgttc gtggtcaaaa taatcaatgg
    12781 acaattgcga ataagcctga ctatgtaacg ttagatgcac aaactggtaa agtgacgttc
    12841 aatgccaata ctataaaacc aaattcatca atcacaatta ctccgaaagc aggtacaggt
    12901 cactcagtaa gtagtaatcc aagtacatta actgcaccgg cagctcatac tgtcaacaca
    12961 actgaaattg tgaaagatta tggttcaaat gtaacagcag ctgaaattaa caatgcagtt
    13021 caagttgcta ataaacgtac tgcaacgatt aaaaatggca cagcaatgcc tactaattta
    13081 gctggtggta gcacaacgac gattcctgtg acagtaactt acaatgatgg tagtactgaa
    13141 gaagtacaag agtccatttt cacaaaagcg gataaacgtg agttaatcac agctaaaaat
    13201 catttagatg atccagtaag cactgaaggt aaaaagccag gtacaattac gcagtacaat
    13261 aatgcaatgc ataatgcgca acaacaaatc aataccgcga aaacagaagc acaacaagtg
    13321 attaataatg agcgtgcaac accacaacaa gtttctgacg cactaactaa agttcgtgca
    13381 gcacaaacta agattgatca agctaaagca ttacttcaaa ataaagaaga taatagccaa
    13441 ttagtaacgt ctaaaaataa cttacaaagt tctgtgaacc aagtaccatc aactgctggt
    13501 atgacgcaac aaagtattga taactataat gcgaagaagc gtgaagcaga aactgaaata
    13561 actgcagctc aacgtgttat tgacaatggc gatgcaactg cacaacaaat ttcagatgaa
    13621 aaacatcgtg tcgataacgc attaacagca ttaaaccaag cgaaacatga tttaactgca
    13681 gatacacatg ccttagagca agcagtgcaa caattgaatc gcacaggtac aacgactggt
    13741 aagaagccgg caagtattac tgcttacaat aattcgattc gtgcacttca aagtgactta
    13801 acaagtgcta aaaatagcgc taatgctatc attcagaagc caataagaac agtgcaagag
    13861 gtacaatctg cgttaacaaa tgtaaatcgt gtcaatgagc gattaacgca agcaattaat
    13921 caattagtac ctttagctga taatagtgct ttaagaactg ctaagacgaa acttgatgaa
    13981 gaaatcaata aatcagtaac tactgatggt atgacacaat catcaatcca agcatatgaa
    14041 aatgctaaac gtgcaggtca aacagaaaca acaaatgcac aaaatgttat taacaatggt
    14101 gacgcgacag accaacaaat tgccgcagaa aaaacaaaag tagaagaaaa atataatagc
    14161 ttaaaacaag caattgctgg attaacacca gacttggcac cattacaaac tgcaaaaact
    14221 cagttgcaaa atgatattga tcagccaacg agtacgactg gtatgacaag cgcatctgtt
    14281 gctgcattta atgacaaact ttcagcagct agaactaaaa ttcaagaaat tgatcgcgta
    14341 ctagcatctc atccagatgt agcaacgatt cgtcaaaacg tgacagcagc gaatgctgct
    14401 aaaacagcac ttgatcaagc gcgcaatggc ttaacagtcg ataaagcacc tttagaaaat
    14461 gcgaaaaatc aactacaaca tagtattgat acgcaaacaa gtacaactgg tatgacacaa
    14521 gactctataa atgcatacaa tgcgaagtta acagctgcac gtaataaggt tcaacaaatc
    14581 aatcaagtat tagcaggttc acctactgta gatcaaatta atacaaatac gtctgcagca
    14641 aatcaagcga aatctgattt agatcatgca cgtcaagcgt taacaccaga taaagcgccg
    14701 cttcaaaatg cgaaaacgca attagaacaa agcattaatc aaccaacaga tacaacaggt
    14761 atgacaaccg cttcgttaaa tgcatacaac caaaaattac aagcagcacg tcaaaagtta
    14821 actgaaatta atcaagtgtt gaatggcaac ccaactgtcc aaaatatcaa tgataaagtg
    14881 gcagaggcaa accaagctaa ggatcaatta aatacagcac gtcaaggttt aacattagat
    14941 agacagccag cgttaacaac attacatggt gcatctaact taaaccaagc acaacaaaat
    15001 aatttcacgc aacaaattaa tgctgctcaa aatcatgctg cgcttgaaac aattaagtct
    15061 aacattacgg ctttaaatac tgcgatgacg aaattaaaag acagtgttgc ggataataat
    15121 acaattaaat caggtcaaaa ttacactgac gcaacaccag ctaataaaca agcctatgat
    15181 aatgcagtta atgcggctaa aggtgtcatt ggagaaacga ctaatccaac gatggatgtt
    15241 aacacagtga accaaaaagc agcatctgtt aaatcgacga aagatgcttt agatggtcaa
    15301 caaaacttac aacgtgcgaa aacagaagca acaaatgcga ttacgcatgc aagtgattta
    15361 aaccaagcac aaaagaatgc attaacacaa caagtgaata gtgcacaaaa cgtgcaagca
    15421 gtaaatgata ttaaacaaac gactcaaagc ttaaatactg ctatgacagg tttaaaacgt
    15481 ggcgttgcta atcataacca agtcgtacaa agtgataatt atgtcaacgc agatactaat
    15541 aagaaaaatg attacaacaa tgcatacaac catgcgaatg acattattaa tggtaatgca
    15601 caacatccag ttataacacc aagtgatgtt aacaatgctt tatcaaatgt cacaagtaaa
    15661 gaacatgcat tgaatggtga agctaagtta aatgctgcga aacaagaagc gaatactgca
    15721 ttaggtcatt taaacaattt aaataatgta caacgtcaaa acttacaatc gcaaattaat
    15781 ggtgcgcatc aaattgatgc agttaataca attaagcaaa atgcaacaaa cttgaatagt
    15841 gcaatgggta acttaagaca agctgttgca gataaagatc aagtgaaacg tacagaagat
    15901 tatgcggatg cagatacagc taaacaaaat gcatataaca gtgcagtttc aagtgctgaa
    15961 acaattatta atcaaacagc taatccgaca atgtctgttg atgatgttaa tcgtgcaact
    16021 tcagctgtta ctactaataa aaatgcatta aatggtgatg aaaaattagt acaatctaaa
    16081 acagatgctg caagagcaat tgatgcatta ccacatttaa ataatgcaca aaaagcagat
    16141 gttaaatcta aaattaatgc tgcatcaaat attgctggtg taaataccgt taaacaacaa
    16201 ggtacagatt taaatacagc gatgggtaac ttgcagggtg caatcaatga tgaacaaacg
    16261 acgcttaata gtcaaaatta tcaagatgcg acacctagta agaaaacagc atacacaaat
    16321 gcggtgcaag ctgcgaaaga tattttaaat aaatcaaatg gtcaaaataa aacgaaagat
    16381 caagttactg aagcgatgaa tcaagtgaat tcggctaaaa ataacttaga tggtacgcgt
    16441 ttattagatc aagcgaagca aacagcgaaa cagcagttaa ataatatgac gcatttaaca
    16501 actgcacaaa aaacgaattt aacaaatcaa attaatagtg gtactactgt tgctggtgtt
    16561 catacggttc aatcaaatgc caacacatta gatcaagcga tgaatacgtt aagacaaagt
    16621 attgctaaca atgatgcgac taaagcaagt gaagattacg tagatgctaa taatgataag
    16681 caaacagcat ataacaacgc ggtagctgct gctgaaacga ttattaatgc gaatagtaat
    16741 ccagaaatga atccaagtac gattacacaa aaagcagagc aagtgaatag ttctaaaacg
    16801 gcacttaacg gtgatgaaaa cttagctacg gcaaaacaaa atgcgaaaac gtacttaaac
    16861 acattaacga gtattacaga tgctcaaaag aacaatttga ttagtcaaat tagtagtgcg
    16921 acaagagtga gtggtgttga tactgtaaaa caaaatgcac aacatttaga tcaagctatg
    16981 gctaacttac aaaatggtat taacaacgaa tctcaagtga aatcatctga gaaatatcgt
    17041 gatgctgata caaataaaca acaagagtat gataatgcta ttactgcagc gaaagcgatt
    17101 ttaaataaat cgacaggtcc aaacactgcg caaaatgcag ttgaagcagc attgcaacgt
    17161 gttaatactg cgaaagatgc attgaatggt gatgcaaaat taattgcagc tcaaaacgca
    17221 gcgaaacaac atttaggtac tttaacgcat atcactacag cacaacgcaa tgatttaaca
    17281 aatcaaattt cacaagctac aaacttagct ggtgttgaat cagttaaaca aagtgcaaat
    17341 agtttagatg gcgcaatggg taacttacaa acggctatca acgataagtc aggaacatta
    17401 gcgagccaaa acttcttgga tgctgatgag caaaaacgta atgcttacaa tcaagctata
    17461 tcagctgccg aaaccatttt aaataaacaa actggaccga atacagcgaa aacagcggtt
    17521 gaacaagcac ttaataatgt taatagtgcg aaacatgcat taaatggtac gcaaaactta
    17581 aataatgcga aacaagcagc gattacagca attaatggcg catctgattt aaatcaaaaa
    17641 caaaaagatg cattaaaagc acaagctaat ggtgctcaac gcgtatctaa tgcaaatgat
    17701 gtacaacgta atgcgactga actgaacacg gcaatgggtc aattacaaca tgccatcgca
    17761 gataagacga atacgttagc aagcagtaaa tatgtcaacg ccgatagcac taaacaaaat
    17821 gcttacacaa ctaaagttac caatgctgaa catattatta gcggtacgcc aacggttgtt
    17881 acaacacctt cagaagtaac agctgcagct aatcaagtaa acagcgcgaa acaagaatta
    17941 aatggtgacg aaagattacg tgttgcaaaa caaaacgcca atactgctat tgatgcatta
    18001 acgcaattaa atactcctca aaaagctaaa ttaaaagaac aagtgggaca agccaataga
    18061 ttagaagacg tacaatctgt tcaaacaaat ggacaatcat tgaacaatgc aatgaaaggc
    18121 ttaagagata gtattgctaa cgaaacaaca gtcaaagcaa gtcaaaacta tacagacgca
    18181 agtccgaata accaatcaac atataatagc gctgtgtcaa atgcgaaagg tatcattaat
    18241 caaactaaca atccaactat ggatactagt gcgattaccc aagctacaac acaagtgaat
    18301 aatgctaaaa atggtttaaa cggtgctgaa aacttaagaa atgcacaaaa cactgctaag
    18361 caaaacttaa atacgttatc acacttaaca aataaccaaa aatctgcaat ctcatcacaa
    18421 attgatcgtg caggtcatgt gagtgaggta acagctgcta aaaatgcagc aactgagtta
    18481 aacgcgcaaa tgggcaactt ggaacaagct atccatgatc aaaacacagt taaacaaggt
    18541 gttaacttca ctgatgcaga taaagctaaa cgtgatgctt atacaaatgc ggtaagcaga
    18601 gcagaaacaa ttctgaataa aacgcaaggt gcaaatacgt ctaaacaaga tgttgaagcg
    18661 gctattcaaa atgttacaag tgctaaaaat gcattgaatg gtgatcaaaa cgttacaaat
    18721 gcgaagaatg cagctaaaaa tgcattaaat aacttaacgt caattaataa tgcacaaaaa
    18781 cgtgacttaa caactaaaat tgatcaagca acaacagtag ctggtgttga agcggtatct
    18841 aatacaggta cacaattgaa tacagcgatg gctaacttgc aaaatggtat taatgataaa
    18901 gcgaatactt tagcgagcga aaactatcat gatgctgatt cagataagaa aactgcttat
    18961 actcaagccg ttacgaacgc agaaaatatt ttaaataaaa atagtggatc aaatttagat
    19021 aaagctgccg ttgaaaacgc gttgtcacaa gtgacaaatg cgaaaggtgc cctaaatggt
    19081 aaccataatt tagagcaagc taaatcaaat gcaaacacta ctataaacgg ccttcaacat
    19141 ttaacaacag cacaaaaaga taaattgaaa caacaagtgc aacaagcaca aaatgttgca
    19201 ggtgtagata ctgttaaatc aagtgccaac acattaaatg gtgctatggg tacgttaaga
    19261 aatagcatac aagataacac agctacgaaa aatggccaaa actatcttga tgctacagaa
    19321 cgtaacaaaa caaactataa caatgctgtt gatagtgcta atggtgtcat taatgcaaca
    19381 agcaatccaa atatggatgc taatgcaatt aaccaaatcg ctacacaagt gacatcaacg
    19441 aaaaatgcat tagatggtac acataattta acgcaagcga aacaaacagc aacaaatgcc
    19501 atcgatggtg ctactaactt aaataaagcg caaaaagatg cgttaaaagc acaagttaca
    19561 agtgcgcaac gtgttgcaaa tgtaacaagt atccaacaaa ctgcaaatga acttaataca
    19621 gctatgggtc aattacaaca tggtattgat gatgaaaatg caacaaaaca aactcaaaaa
    19681 tatcgtgacg ctgaacaaag taagaaaact gcttatgatc aagctgtagc tgctgcgaaa
    19741 gcaattttaa ataaacaaac aggttccaat tcagataaag cagcagttga ccgtgcatta
    19801 caacaagtaa caagtacgaa agatgcattg aatggggatg ctaaactggc agaagcgaaa
    19861 gcggcagcta gacaaaactt aggtacttta aaccatatta cgaatgcaca acgtactgcg
    19921 ttagaaggtc aaatcaatca agcgacgact gttgatggcg ttaatactgt aaaaacaaat
    19981 gccaatacat tagacggcgc tatgaatagc ttacaaggtg caatcaatga taaagatgcg
    20041 acattaagaa atcaaaatta tcttgatgca gatgaatcaa aacgaaatgc atatacgcaa
    20101 gctgtcacag cggctgaagg cattttaaat aaacaaacag gtggtaacac atctaaagca
    20161 gacgttgata atgcattaaa tgcagttaca agagcgaaag cggctttaaa tggtgctgaa
    20221 aacttaagaa atgcgaaaac ttcagcaaca aatacgatta atggtttacc taacttaaca
    20281 caattacaaa aagacaactt gaagcatcaa gttgaacaag cgcaaaatgt agttggtgta
    20341 aatggtgtta aagataaagg taatacatta aatactgcca tgggtgcatt acgtacaagt
    20401 atccaaaatg ataatacgac gaaaacaagt caaaattatc ttgatgcatc tgatagcaac
    20461 aaaaataatt acaatactgc tgtaaataat gcaaatggtg ttattaatgc aacgaacaat
    20521 ccaaatatgg atgctaatgc gattaatgac atggcaaatc aagtcaatac aacaaaagca
    20581 gcgttaaatg gtgcacaaaa cttagctcaa gctaaaacaa atgcgacgaa cacaattaac
    20641 aacgcgcaag acttaaacca aaaacaaaaa gatgcattaa aaacacaagt taacaatgca
    20701 caacgtgtat ctgatgcaaa taacgttcaa catacagcta ctgaattgaa cggtgcgatg
    20761 acagtactta aagcagctat tgcggataaa gaaagaacaa aagcaagcgg taattatgtc
    20821 aatgctgatc aagaaaaacg tcaagcgtat gattcaaaag tgactaacgc tgaaaatatc
    20881 attaatggta caccaaatgc gacattaaca gtcaatgacg taaatagtgc ggcatcacaa
    20941 gtcaatgcgg ctaaaacagc attaaatggt gataacaact tacgtgtagc gaaagagcat
    21001 gctaacaata caattgacgg cttagcacaa ttgaataatg tacaaaaagc aaaattaaaa
    21061 gaacaagttc aaagtgcaac tacattagat ggtgttcaaa ctgttaaaaa tagttctcaa
    21121 acgttgaata cagcgatgaa aggcttaaga gatagtattg cgaatgaagc aacgattaaa
    21181 gcaggtcaaa actacactga cgcaagtcca aataatcgta acgagtacga cagcgcagtt
    21241 actgcagcaa aagcaatcat taatcaaaca tcgaacccaa cgatggaacc aaatactatt
    21301 acgcaagcaa catcacaagt gacaactaaa gaacatgcat taaatggtgc gcaaaactta
    21361 gctcaagcta agacaacagc gaaaaacaac ttgaataact taacatcaat taacaatgca
    21421 caaaaagatg cgttaacgcg taacattgat ggtgcaacta cagtagctgg tgtaaatcaa
    21481 gaaactgcaa aagcaacaga attaaataac gcaatgcaca gtttacaaaa tggtatcaat
    21541 gatgagacac aaacaaaaca aactcagaaa tacctagatg ctgagccaag taagaaatca
    21601 gcttatgatc aagcagtaaa tgcagcaaaa gcaattttaa caaaagctag tggtcaaaat
    21661 gtagacaaag cagcagttga acaagcatta caaaatgtga acagtacgaa gacggcgttg
    21721 aacggtgatg cgaaattaaa tgaagctaaa gctgctgcga aacaaacgtt aggtacatta
    21781 acacacatta ataatgcaca acgtaatgcg ttagataatg aaattacaca agcaacaaat
    21841 gttgaaggtg ttaatacagt taaagccaaa gcgcaacaat tagatggtgc tatgggtcaa
    21901 ttagaaacat caattcgtga taaagacacg acgttacaaa gtcaaaatta tcaagatgct
    21961 gatgatgcta aacgaacggc ttattctcaa gcagtaaatg cagcagcaac tattttaaat
    22021 aaaacagctg gaggaaatac acctaaagca gatgtcgaaa gagcaatgca agctgttaca
    22081 caagccaata ctgcattaaa cggtattcaa aacttagaac gtgcgaaaca ggctgcgaac
    22141 acagcgatta caaatgcttc ggacttaaat acaaaacaaa aagaagcatt gaaagcacaa
    22201 gtaacaagtg caggacgcgt atctgcagca aatggtgttg aacatactgc gactgaatta
    22261 aatactgcga tgacagcttt aaaacgtgcc attgctgata aagctgacac aaaagctagt
    22321 ggtaattatg tcaatgctga tgcgaataaa cgccaagcat atgatgaaaa agtgacagct
    22381 gcagaacata tcgttagtgg tacaccaaca ccaacgttaa caccatcaga tgttacaaat
    22441 gcagcaacgc aagtaacgaa tgcgaagacg cagttaaacg gtaatcataa tttagaagta
    22501 gcgaaacaaa atgctaacac agcaattgat ggtttaactt ctttaaatgg tccgcaaaaa
    22561 gcaaaactta aagaacaagt gggtcaagcg acgacgttgc caaatgttca aactgttcgt
    22621 gataatgcac aaacattaaa cactgcaatg aaaggtctac gagatagcat tgcgaatgaa
    22681 gcaacgatta aagcaggtca aaactacaca gatgcaagtc aaaacaaaca aaatgactac
    22741 aacaatgcag tcactgcagc aaaagcaatc attggtcaaa caactagtcc atcaatgatt
    22801 gcgcaagaaa ttaatcaagc gaaagaccaa gtgacagcta aacaacaagc gttaaacggt
    22861 caagaaaact taagaactgc gcaaacaaat gcgaagcaac atttgaatgg cttaagtgac
    22921 ttaactaatg cacaaaaaga tgcagcgaaa cgccaaatcg aaggtgcaac gcatgttaat
    22981 gaagtaacac aagcgcaaaa taatgcggac gcattaaata cagctatgac gaacttgaaa
    23041 aatggtattc aagatcaaaa tacgattaag caaggtgtta acttcactga tgcagatgaa
    23101 gcgaaacgta atgcatatac aaatgcagtg acgcaagctg aacaaatttt aaataaagca
    23161 caaggtccaa atactgcaaa agacggtgtc gaaactgcgt tacaaaatgt acaacgtgct
    23221 aaaaacgaat tgaacggtaa tcaaaatgtt gcgaacgcta agacaactgc gaaaaatgca
    23281 ttgaataacc ttacatcaat taataatgca caaaaagcag cattgaaatc acaaattgaa
    23341 ggtgcgacaa cagttgcagg tgtaaatcaa gtgtctacaa tggcatctga attaaatact
    23401 gcaatgagca acttacaacg tggtattaat gacgaagcag ctacaaaagc agctcagaaa
    23461 tatactgaag cagatagaga taaacaaact gcatacaatg atgctgtaac agcagctaaa
    23521 acgttattag ataaaacagc tggttcaaat gacaataaag tagccgttga acaagcatta
    23581 caacgtgtga atactgctaa aacagcatta aatggtgacg cgcgattaaa tgaagcgaag
    23641 aacacagcta aacaacaatt agcgacaatg tcacatttaa ctaatgctca aaaagcaaac
    23701 ttaacagaac aaattgaacg tggtacaact gttgctggtg ttcaaggcat ccaagcaaat
    23761 gctggtactt taaatcaagc aatgaatcaa ttaagacaaa gtattgcttc taaagatgcg
    23821 actaaatcaa gcgaagatta tcaagacgcg aatgcagatt tacaaaatgc atacaatgat
    23881 gcggtaacta atgctgaagg tattattagt gcaacgaata accctgaaat gaatcctgat
    23941 acaattaacc aaaaagcgag ccaagtgaac agtgcgaagt ctgcattgaa cggtgatgaa
    24001 aaattagcag cagcaaaaca aactgcgaaa tcagatatcg gtcgtttgac agacttgaac
    24061 aatgcacaac gaactgcggc aaatgctgaa gtggatcaag caccaaatct tgcagctgtc
    24121 acagcggcta aaaataaagc aacatcgtta aacacagcga tgggtaattt gaaacatgca
    24181 cttgctgaaa aggataatac gaaacgtagt gtcaattaca cagatgcgga tcaaccaaaa
    24241 caacaagcgt atgatactgc agttacacaa gcagaagcaa ttactaatgc aaatggcagt
    24301 aacgcgaatg aaacacaagt tcaagcagcg cttaaccaat tgaatcaagc taaaaacgac
    24361 ttgaatggtg ataataaagt tgctcaagcg aaagaaacag caaaacgtgc attagcttca
    24421 tatagtaact tgaataacgc gcaatcaact gcagcaacta gtcaaattga caatgcaacg
    24481 acagtagcag acgtaactgc tgcacaaaat actgctaatg aattaaatac agcaatgggt
    24541 caacttcaaa atggtattaa tgaccaaaac actgttaaac aacaagtgaa ctttacagat
    24601 gctgaccaag gtaagaaaga tgcttacaca aatgctgtta cgaatgctca aggtatttta
    24661 gataaagcaa acggtcaaaa tatgacaaaa gcacaagttg aagctgcatt aaatcaagta
    24721 acgactgcta agaatgcttt aaacggtgat gcaaatgtaa gacaagcaaa atcagatgcg
    24781 aaagcaaact taggtacatt aacacactta aataatgcac aaaaacaaga tttaacatca
    24841 caaatcgaag gtgcaacaac agtcaacggt gtaaatagtg ttaaaacgaa agcacaagac
    24901 ttagatggtg caatgcaacg attagagtca gcaatcgcaa ataaagatca aactaaagcg
    24961 agcgaaaact acattgacgc agatccaact aagaaaacag catttgataa tgccatcaca
    25021 caagctgaat cttacttaaa taaagatcat ggtacgaata aagataagca agctgttgaa
    25081 caagcaattc aaagtgtaac gtctactgaa aatgctttga acggtgacgc gaacttacaa
    25141 cgcgctaaaa ctgaagctac acaagctatc gataacttga cacaattgaa tacaccgcaa
    25201 aaaacagcat tgaaacaaca agtgaatgct gcacaacgcg tatcaggtgt aactgatctg
    25261 aaaaatagtg ctacatcact taataatgcg atggatcaat taaaacaagc aattggtgat
    25321 catgacacaa ttgtagctgg tggtaattac actaacgcaa gtcctgataa acaaggtgct
    25381 tacactgatg catataatgc tgcgaagaat atcgtaaatg gttcacctaa tgtgattaca
    25441 aatgcagcag atgttactgc ggcaacacaa cgtgtcaata atgctgaaac aagtttaaat
    25501 ggtgatacaa acttagcaac tgcgaagcaa caagctaaag atgcattacg tcaaatgaca
    25561 catttatctg atgcacaaaa acaaagtatt actggtcaaa ttgatagcgc gacacaagta
    25621 actggtgtac aaagtgtgaa agacaatgca acaaatcttg acaatgcaat gaatcaactt
    25681 cgaaatagta ttgcgaataa agatgaagta aaagcgagtc aaccatatgt tgatgcagat
    25741 acagataaac aaaatgcata caatacagca gttacaagtg ctgaaaatat cattaatgca
    25801 acgagtcagc caacacttga tccatctgca gtaacacaag cagctaatca agtgaacact
    25861 aacaaaactg cgcttaatgg tgcgcaaaac ttagcaaata aaaagcaaga aacaactgct
    25921 aacatcaacc gattaagtca tttaaacaat gctcaaaagc aagatttaaa tacacaagtg
    25981 acaaatgcac caaatattag cacagtaaat caagtgaaaa ctaaagctga acaattagat
    26041 caagcaatgg aacgtttaat caacggaatc caagacaaag atcaagtgaa acaaagtgtt
    26101 aactttacag atgcagatcc agaaaaacaa acagcataca acaatgcggt aactgctgct
    26161 gaaaatatta ttaatcaagc aaatggtaca aatgcgaacc aatcacaagt tgaagcagca
    26221 ctttcaactg taacaactac taaacaagcg ttgaatggtg atagaaaagt aacagatgct
    26281 aaaaacaatg caaaccaaac attatctacg ttagataact taaacaatgc acaaaaaggt
    26341 gctgttactg gaaacatcaa tcaagcgcac actgtagctg aagtaacgca agccattcaa
    26401 accgctcagg aactgaatac agcgatgggt aacttgaaaa atagcttgaa tgataaagac
    26461 actacacttg gcagtcaaaa ctttgcagat gcagatccag agaagaaaaa tgcatacaat
    26521 gaagcggttc gtaatgctga aaatatttta aataaatcta caggtacgaa cgtgcctaaa
    26581 gatcaagttg aagcagctat gaatcaagtg aatactacaa aagcagcgct taatggtact
    26641 caaaaccttg aaaaagcgaa acaacacgca aatacagcaa ttgacggttt aagccattta
    26701 acaaatgcac aaaaagaggc attaaaacaa ttggtacaac aatcgactac tgttgcagaa
    26761 gcacaaggta atgaacaaaa agcaaacaat gttgatgcag caatggacaa attacgtcaa
    26821 agtattgcag ataatgcgac aacaaaacaa aaccaaaatt atactgatgc aagtccgaat
    26881 aaaaaggatg cgtacaataa tgctgtcaca actgcacaag gtattattga tcaaactaca
    26941 aacccttcat tagatccgac tgttatcaat caagctgctg gacaagtaag cacgtctaaa
    27001 aatgctttaa atggtaatga aaacttagag gcagcgaagc aacaagcaac gcaatcttta
    27061 ggttcattag acaacttaaa taatgcgcaa aaacaagctg ttactaatca aattaatggc
    27121 gcgcatactg ttgatgaagc aaatcaaatt aagcaaaatg cgcaaaactt aaatactgcg
    27181 atgggtaact tgaaacaagc gatagctgat aaagatgcta cgaaagcaac agttaacttc
    27241 actgatgcag atcaagcaaa acaacaagca tataacactg cagttacaaa tgctgaaaat
    27301 atcatttcaa aagctaatgg tggtaatgca acacaaactg aagttgaaca agcaatccaa
    27361 caagtaaatg cagcaaaaca agcattaaat ggtaatgcca acgttcaaca tgcaaaagac
    27421 gaagcaacag cattaattaa taactctaat gatcttaacc aagcacagaa agatgcatta
    27481 aaacaacaag tacaaaatgc aactactgta gctggtgtaa acaatgttaa acaaacggcg
    27541 caagagttaa acaatgcgat gacacaatta aaacaaggca ttgcagataa agaacaaaca
    27601 aaagctgatg gtaactttgt caatgcagat tctgacaagc aaaatgcata taatcaagca
    27661 gtagcgaaag ctgaagcatt aattagtggt acgcctgatg ttgtcgttac acctagcgaa
    27721 attactgcag cgttaaataa agttacgcaa gctaaaaatg atttaaatgg taatacaaac
    27781 ttagcaacgg cgaaacaaaa tgttcaacat gctattgatc aattgccaaa cttaaaccaa
    27841 gcgcaacgtg atgaatacag caaacaaatc acgcaagcaa cacttgtacc aaacgtcaat
    27901 gctattcaac aagcggcaac aacgcttaat gacgcgatga cacaattgaa acaaggtatt
    27961 gcgaataaag cacaaattaa aggtagcgag aactatcacg atgctgatac tgacaagcaa
    28021 acagcatatg ataatgcagt aacaaaagca gaagaattgt taaaacaaac aacaaatcca
    28081 acaatggatc caaatacaat tcaacaagca ttaactaaag tgaatgacac aaatcaagca
    28141 cttaacggta atcaaaaatt agctgatgcc aaacaagatg ctaagacaac acttggtaca
    28201 ctagatcatt taaatgatgc tcaaaaacaa gcgctaacaa ctcaagttga acaagcacca
    28261 gatattgcaa cagttaataa tgttaagcaa aatgctcaaa atctgaataa tgctatgact
    28321 aacttaaaca atgcattaca agataaaact gagacattaa atagcattaa ctttactgat
    28381 gcagatcaag ctaagaaaga tgattatact aatgcggttt cacatgcaga aggtatttta
    28441 tctaaagcaa atggcagcaa tgcaagtcaa actgaagtgg aacaagcgat gcaacgtgtg
    28501 aacgaagcga aacaagcatt gaatggtaat gacaatgtac aacgtgcaaa agatgcagcg
    28561 aaacaagtaa ttacaaatgc aaatgattta aatcaagcgc aaaaagatgc attaaaacaa
    28621 caagtcgatg ctgcgcaaac tgttgcaaat gtaaacacga ttaagcaaac agcacaagat
    28681 ttaaatcaag caatgacaca attgaaacaa ggtattgcag ataaagacca aactaaagca
    28741 aatggtaact ttgtcaatgc tgatactgat aagcaaaatg catataacaa tgcggtagcg
    28801 catgctgaac aaatcattag tggtacacca aatgcaaacg tggatccaca acaagtggct
    28861 caagcgttac aacaagtgaa tcaagctaag ggtgatttaa acggtaacca taacttacaa
    28921 gttgctaaag acaatgcaaa tacagccatt gatcagttac caaacttaaa tcaaccacaa
    28981 aaaacagcat taaaagacca agtgtcgcat gcagaacttg ttacaggtgt taatgctatt
    29041 aagcaaaatg ctgatgcgtt aaataatgca atgggtacgt tgaaacaaca aattcaagcg
    29101 aatagtcaag taccacaatc agttgacttt acacaagcgg atcaagacaa acaacaagct
    29161 tataacaatg cagctaacca agcgcaacaa atcgcaaatg gcacaccaac acctgtattg
    29221 gcgcctgata cagtaacaaa agcagttaca actatgaatc aagcgaaaga tgcattaaac
    29281 ggtgatgaaa aattagcgca agcgaaacaa gatgctttag caaatcttga tacgttacgt
    29341 gacttaaatc aaccacaacg tgatgcatta cgaaaccaaa tcaatcaagc acaagcttta
    29401 gctacagttg aacaaactaa acaaaatgca caaaatgtga atacagcaat gggtaacttg
    29461 aaacaaggta ttgcaaataa agatactgtg aaagcaagtg agaactacca cgatgctgat
    29521 gtcgataagc aaacagcata tacaaatgca gtgtctcaag cggaaggtat tatcaatcaa
    29581 acgacaaatc caacgcttaa cccagatgac attactcgtg cattaactca agtgactgat
    29641 gctaaaaata gcttaaacgg tgaagctaaa ttagccactg aaaagcaaaa tgctaaagat
    29701 gccgtaagtg gaatgacgca tttaaacgat gctcaaaaac aagcattaaa aggtcaaatc
    29761 gatcaatcgc ctgaaattgc tacagtgaac caagttaaac aaacagcaac gagcctagat
    29821 caagcaatgg atcaattatc acaagctatt aatgataaag atcaaatatt agcggacggt
    29881 aattacttaa atgcagatcc tgacaaacaa aatgcgtata aacaggcagt agcaaaagct
    29941 gaagcattat tgaataaaca aagtggtact aatgaagtac aagcacaagt tgaaagcatc
    30001 actaatgaag tgaacgcagc gaaacaagca ttaaatggta atgacaattt ggcaaatgca
    30061 aaacaacaag caaaacaaca attggcgaac ttaacacact taaatgatgc acaaaaacaa
    30121 tcatttgaaa gtcaaattac acaagcgcca cttgttacag atgtcactac gattaatcaa
    30181 aaagcacaaa cgttagatca tgcgatggaa ttattaagaa atagtgttgc ggataatcaa
    30241 acgacattag cgtctgaaga ttatcatgat gcaactgcgc aaagacaaaa tgactataac
    30301 aaagctgtaa cagctgctaa taatatcatt aatcaaacta catcgcctac gatgaatcca
    30361 gatgatgtta atggtgcaac gacacaagtg aataatacga aagttgcatt agatggtgat
    30421 gaaaaccttg cagcagctaa acaacaagca aacaacagac ttgatcaatt agatcatttg
    30481 aataatgcgc aaaagcaaca gttacaatca caaattacgc aatcatctga tattgctgca
    30541 gttaatggtc acaaacaaac agcagaatct ttaaatactg cgatgggtaa cttaattaat
    30601 gcgattgcag atcatcaagc cgttgaacaa cgtggtaact tcatcaatgc tgatactgat
    30661 aaacaaactg cttataatac agcggtaaat gaagcagcag caatgattaa caaacaaact
    30721 ggtcaaaatg cgaaccaaac agaagtagaa caagctatta ctaaagttca aacaacactt
    30781 caagcgttaa atggagatca taatttacaa gttgctaaaa caaatgcgac gcaagcaatt
    30841 gatgctttaa caagcttaaa tgatcctcaa aaaacagcat taaaagacca agttacagct
    30901 gcaactttag taactgcagt tcatcaaatt gaacaaaatg cgaatacgct taaccaagca
    30961 atgcatggtt taagacagag cattcaagat aacgcagcaa ctaaagcaaa tagcaaatat
    31021 atcaacgaag atcaaccaga gcaacaaaac tatgatcaag ctgttcaagc cgcaaataat
    31081 attatcaatg aacaaactgc aacattagat aataatgcga ttaatcaagt agcggcaact
    31141 gtgaatacaa cgaaagcagc attacatggt gatgtgaaat tacaaaatga taaagatcat
    31201 gctaaacaaa cggttagcca attagcacat ctaaacaatg cacaaaaaca tatggaagat
    31261 acgttaattg atagtgaaac aactagaaca gcagttaagc aagatttgac tgaagtacaa
    31321 gcattagatc aacttatgga tgcattacaa caaagtattg ctgacaaaga tgcaacacgt
    31381 gcgagcagtg catatgtcaa tgcagaaccg aataaaaaac aagcctatga tgaagcagtt
    31441 caaaatgctg agtctatcat tgcaggatta aataatccaa ctatcaataa aggtaatgta
    31501 tcaagtgcga ctcaagcagt aatatcatct aaaaatgcat tagatggtgt tgaacgatta
    31561 gctcaagata agcaaactgc tggaaattct ctaaatcatt tagatcaatt aacaccagct
    31621 caacaacaag cgctagaaaa tcaaattaat aatgcaacaa ctcgtgataa agtggctgaa
    31681 atcattgcac aagcgcaagc attaaatgaa gcgatgaaag cattaaaaga aagtattaag
    31741 gatcaaccac aaactgaagc aagtagtaaa tttattaacg aggatcaagc gcaaaaagat
    31801 gcatatacgc aagcagtaca acacgcgaaa gatttgatta acaaaacaac tgatcctaca
    31861 ttagctaaat caatcattga tcaagcgaca caggcagtga ctgatgctaa aaacaattta
    31921 catggtgatc aaaaactagc tcaagataag caacgtgcaa cagaaacgtt aaataacttg
    31981 tctaacttga atacaccaca acgtcaagca cttgaaaatc aaatcaataa tgcagcaact
    32041 cgtggtgaag tagcacaaaa attaactgaa gcacaagcac ttaaccaagc aatggaagct
    32101 ttacgtaata gcattcaaga tcaacaacaa acagaatctg gtagcaagtt tattaatgaa
    32161 gataaaccgc aaaaagatgc ttaccaagca gcagttcaaa atgcaaaaga tttaattaac
    32221 caaacaggta atccaacgct tgataaagca caagttgaac aattgacaca tgcttttaaa
    32281 caagctaaag ataacctaca cggtgatcaa aaacttgcag acgataaaca acatgcggtt
    32341 actgatttaa atcaattaaa tggtttgaat aatccgcaac gtcaagcact tgaaagccaa
    32401 ataaacaacg cagcaactcg tggcgaagta gcgcaaaaat tagctgaagc aaaagcgctt
    32461 gatcaagcaa tgcaagcatt acgaaatagt attcaagatc aacaacaaac ggaagcgggt
    32521 agcaagttta tcaatgaaga taaaccgcaa aaagatgctt accaagcagc agttcaaaat
    32581 gcaaaagatt taattaacca aacaggtaat ccaacactcg acaaatcaca agtagaacaa
    32641 ttaacacaag cagtaacaac tgcaaaagat aatctacatg gtgatcaaaa acttgctcgt
    32701 gatcaacaac aagcagtaac aactgtaaat gcattgccaa acttaaatca tgcacaacaa
    32761 caaacattaa ctgatgctat aaatgcagcg cctacaagaa cagaggttgc acaacatgtt
    32821 caaactgcta ctgaacttga tcacgcgatg gaaacattga aaaataaagt tgatcaagtg
    32881 aatacagata aggctcaacc aaattacact gaagcgtcaa ctgataaaaa agaagcagta
    32941 gatcaagcgt tacaagctgc acaaagcatt acagatccaa ctaatggttc aaatgcgaat
    33001 aaagacgctg tagaacaagc attaactaag cttcaagaaa aagtgaatga gttaaatggt
    33061 aatgagagag tcgctgaagc taaaacacaa gcgaaacaaa ctattgacca attaacacat
    33121 ttaaatgctg atcaaattgc aactgctaaa caaaatattg atcaagcgac gaaacttcaa
    33181 ccaatcgctg aattagtaga tcaagcaacg caattgaacc aatcaatgga tcaattacaa
    33241 caagcagtta atgaacatgc taacgttgag caaactatag attacacaca agcagattca
    33301 gataagcaaa aggcttataa acaagcgatt gctgatgctg aaaatgtatt gaaacaaaat
    33361 gcgaataagc aacaagtgga tcaagcactt caaaatattt taaatgcaaa acaagcatta
    33421 aatggtgatg aacgtgtagc acttgctaaa acaaatggta aacatgacat cgaccaattg
    33481 aatgcattaa acaatgctca acaagatgga tttaaaggtc gcatcgatca atcaaacgat
    33541 ttaaatcaaa tccaacaaat tgtagatgag gctaaggcac ttaatcgtgc aatggatcaa
    33601 ttgtcacaag aaatcactgg caatgaagga cgcacgaaag gtagcacgaa ctatgtcaat
    33661 gcagatacac aagtcaaaca agtatatgat gaagcgattg ataaagcgaa acaagcactt
    33721 gataaatcgt ctgggcaaaa cttaactgca gaacaagtta tcaaattaaa tgatgcagtc
    33781 actgcagcta agaaagcatt aaatggtgaa gaaagactta ataatcgtaa agctgaagca
    33841 ttacaaagat tggatcaatt aacacatcta aacaatgctc aaagacaatt agcaatccaa
    33901 caaattaata atgctgaaac gctaaataaa gcatctcgag caattaatag agcaactaaa
    33961 ttagataatg caatgggtgc agtacaacaa tatattgacg aacagcacct tggtgttatc
    34021 agcagcacaa attacatcaa tgcagatgac aatttgaaag caaattatga taatgcaatt
    34081 gcgaatgcag cacatgagtt agataaagtg caaggtaatg caattgcaaa agctgaagca
    34141 gagcaattga aacaaaatat tatcgatgct caaaatgcat taaatggaga ccaaaacctt
    34201 gcaaatgcca aagataaagc aaatgcgttt gttaattcgt taaatggatt aaatcaacag
    34261 caacaagatc ttgcacataa agcaattaac aatgccgata ctgtatcaga tgtaacagat
    34321 attgttaata atcaaattga cttaaatgat gcaatggaaa cattgaaaca tttagttgac
    34381 aatgaaattc caaatgcaga gcaaactgtc aattaccaaa acgctgacga taatgctaaa
    34441 acaaacttcg atgatgccaa acgtctagca aatacattgc taaatagtga taacacaaat
    34501 gtgaatgata tcaatggcgc aatccaagca gtcaatgatg caatccataa tcttaatggt
    34561 gatcaacgac tacaagatgc taaagacaag gcaattcaat caattaatca agctttagct
    34621 aataagctaa aagaaatcga agcttcaaat gcgacggatc aagacaagct tattgcgaaa
    34681 aataaagcag aagaattggc aaacagcatc atcaacaaca ttaataaagc aacaagtaat
    34741 caggctgtat ctcaagttca aacagcaggc aaccacgcga ttgaacaagt gcatgctaat
    34801 gaaataccaa aagcaaaaat tgatgccaat aaagacgttg ataagcaagt tcaagcatta
    34861 attgacgaaa ttgatcgaaa tccaaatcta acagataagg aaaaacaagc acttaaagat
    34921 cgtattaatc aaatacttca acaaggtcat aacgacatta acaatgcgct gactaaagaa
    34981 gaaattgaac aagctaaagc acaacttgcg caagcattac aagacatcaa agatttagtg
    35041 aaagctaaag aagatgcgaa acaagatgtt gataaacaag ttcaagcatt aattgacgaa
    35101 atcgatcaaa atccaaatct aacagataag gaaaaacaag cacttaaaga tcgtattaat
    35161 caaatacttc aacaaggtca taacggcatt aacaatgcga tgactaaaga agaaattgaa
    35221 caagccaaag cacaacttgc acaagcatta aaagaaatta aagatttagt gaaagctaaa
    35281 gaaaatgcga aacaagatgt tgataaacaa gttcaagcat taattgacga aatcgatcaa
    35341 aatccaaatc taacagataa ggaaaaacaa gcgcttaaag atcgaatcaa tcaaatactg
    35401 caacaaggtc ataacgacat taacaatgcg atgactaaag aagaaattga acaagccaaa
    35461 gcacaacttg cacaagcatt acaagacatc aaagatttag tgaaagctaa agaagatgcg
    35521 aaaaatgcaa taaaagcctt agctaatgcg aagcgtgatc aaatcaattc aaatccagat
    35581 ttaacacctg agcaaaaagc aaaagcgctc aaagaaattg acgaagctga aaaacgagca
    35641 ctacaaaacg ttgagaatgc tcaaactata gatcaattaa atcgaggatt aaacttaggt
    35701 ttagatgaca ttagaaatac acatgtatgg gaggttgatg aacaacctgc tgtaaatgaa
    35761 atttttgaag caacacctga gcaaatccta gttaatggtg aactcattgt acatcgtgat
    35821 gacatcatta cagaacaaga tattcttgca cacataaact taattgatca gctttcagca
    35881 gaagttattg atacaccatc aactgcaacg atttctgata gcttaacagc aaaagttgaa
    35941 gttacattgc ttgatggatc aaaagtgatt gttaatgttc ctgtaaaagt tgtagaaaaa
    36001 gaattgtcag tagtcaaaca acaggcaatt gaatcaatcg aaaatgcggc acaacaaaag
    36061 attgatgaaa tcaataatag tgtgacatta acactggaac aaaaagaagc tgcaattgca
    36121 gaagttaata agcttaaaca acaagcaatt gatcatgtta acaatgcacc tgatgttcat
    36181 tcagttgaag aaattcaaca acaagaacaa gcgtatattg aacaatttaa tccagaacaa
    36241 tttacgattg aacaagcaaa atcaaatgca attaaatcga ttgaagatgc aattcaacat
    36301 atgattgatg aaatcaaagc tcgtactgat ctaacagata aagagaagca agaagctatt
    36361 gctaagttaa atcaattaaa agaacaagca attcaagcga ttcaacgtgc gcaaagcatc
    36421 agtgaaataa ctgagcaatt ggaacaattt aaagctcaaa tgaaagcagc taatccaaca
    36481 gcaaaagaac tagctaaacg caagcaagaa gctattagta gaattaaaga cttttcaaat
    36541 gaaaaaataa atagtattcg aaatagtgaa attggcacag ctgatgaaaa acaagcagca
    36601 atgaatcaaa ttaacgaaat tgtgcttgaa acaattagag atattaataa tgcgcataca
    36661 ttacagcaag ttgaggctgc attgaacaat ggtattgctc gaatttcagc agtacaaatt
    36721 gtaatatctg atcgtgctaa acaatcgtca agtactggaa atgaatctaa tagccattta
    36781 acaattggtt atggaactgc aaatcatcca tttaacagtt cgactattgg acataaaaag
    36841 aaacttgatg aagatgatga cattgatcca cttcatatgc gtcactttag taataatttc
    36901 ggtaatgtta ttaaaaacgc tattggtgtg gtgggtatct ctggcttact agctagtttc
    36961 tggttcttca ttgccaaacg tcgtcgtaaa gaagatgaag aggaagaatt agaaataaga
    37021 gataataata aagattcaat aaaagagact ttagacgata caaaacattt accactttta
    37081 tttgcgaaac gtcgcagaaa agaagatgaa gaagatgtta ctgttgaaga aaaagattcg
    37141 ctaaataatg gcgagtcact cgataaagtt aaacatacgc cgttcttctt accaaaacgt
    37201 cgtcgtaaag aagatgaaga agatgtggaa gttacaaatg aaaacacaga tgaaaaagtg
    37261 ttgaaagata acgaacattc accactctta ttcgcaaaac gacgcaaaga taaagaggaa
    37321 gatgttgaaa caacaactag tattgaatct aaagatgagg acgttccttt attattggct
    37381 aaaaagaaaa atcaaaaaga taaccaatcc aaagacaaaa agtcagcatc aaaaaatact
    37441 tctaaaaagg tagcagctaa aaagaagaaa aagaaatc
//
LOCUS       DAOBBH010000006        37281 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.006, whole genome shotgun sequence.
ACCESSION   DAOBBH010000006 DAOBBH010000000
VERSION     DAOBBH010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 37281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37281
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.006"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..933)
                     /locus_tag="P6L01_000171"
     CDS             complement(<1..933)
                     /locus_tag="P6L01_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDP3029273.1"
                     /translation="MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRAKR
                     RFMYKEEAMKALEKMAPQTAGEYEGTNYQFKMPVTVDKHYGSTVYNVNDKQDKHQRVV
                     LYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQDYQATYVLFEKLYHDL
                     LNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPD
                     YLKKDPMVGLDGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLYPDA
                     LNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERR"
     gene            complement(1054..1410)
                     /locus_tag="P6L01_000172"
     CDS             complement(1054..1410)
                     /locus_tag="P6L01_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDP3029274.1"
                     /translation="MVKQLNSVEAFREFIHQYPLAVVHVMRDQCSVCHAVLPQIEDLM
                     QSYPNVPLAVINQSQVEAIAGELNIFTVPVDLIFMNGKEMHRQGRFIDMQRFEHHLKQ
                     MNDSVNNDVDEHEYRK"
     gene            complement(1452..1862)
                     /locus_tag="P6L01_000173"
     CDS             complement(1452..1862)
                     /locus_tag="P6L01_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDP3029275.1"
                     /translation="MTTEGLLVAEKEIEVNGYDIDAMGVVSNIVYIRWFEDLRTAFIN
                     QHMNYSTMINQGISPILMKTEAEYKVPVTIHDKPVGRIYLVKASKMKWVFQFEIVSAH
                     CVHCIGTQTGGFYRLSDKKITSVPQVFQDILATK"
     gene            complement(2242..4308)
                     /locus_tag="P6L01_000174"
     CDS             complement(2242..4308)
                     /locus_tag="P6L01_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HDP3029276.1"
                     /translation="MFKKLFGQLQRIGKALMLPVAILPAAGILLAFGNAMHNEQLVEI
                     APWLKNDIIVMISSVMEAAGQVVFDNLPLLFAVGTALGLAGGDGVAALAALVGYLIMN
                     ATMGKVLHITIDDIFSYAKGAKELSQAAKEPAHALVLGIPTLQTGVFGGIIMGALAAW
                     CYNKFYNITLPPFLGFFAGKRFVPIVTSVVAIATGVLLSFAWPPIQDGLNSLSNFLLN
                     KNLTLTTFIFGIIERSLIPFGLHHIFYSPFWFEFGSYTNHAGELVRGDQRIWMAQLKD
                     GVPFTAGAFTTGKYPFMMFGLPAAAFAIYKNARPERKKVVGGLMLSAGLTAFLTGITE
                     PLEFSFLFVAPVLYGIHVLLAGTSFLVMHLLGVKIGMTFSGGFIDYILYGLLNWDRSH
                     ALLVIPVGIVYAIVYYFLFDFAIRKFKLKTPGREDEETEIRNSSVAKLPFDVLDAMGG
                     KENIKHLDACITRLRVEVVDKSKVDVAGIKALGASGVLEVGNNMQAIFGPKSDQIKHD
                     MAKIMSGEITKPSETTVTEEMSDEPVHVEALGTTDIYAPGVGQIIPLSEVPDQVFAGK
                     MMGDGIGFIPEKGEIVAPFDGTVKTIFPTKHAIGLESESGVEVLIHIGIDTVKLNGEG
                     FESLINVDEKVTQGQPLMKVNLAYLKAHAPSIVTPMIITNLENKELVIEDVQGADPGK
                     LIMTVK"
     gene            complement(4609..6348)
                     /locus_tag="P6L01_000175"
     CDS             complement(4609..6348)
                     /locus_tag="P6L01_000175"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDP3029277.1"
                     /translation="MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQF
                     KFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSG
                     QTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
                     CPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAK
                     DELREFIEMAKIPVIHSLPAKTILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGT
                     NYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQLTENIKHVAERPF
                     LNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTR
                     YLNLGVNNKFIISSWLGTMGCGLPGAMASKIAYPNRQAIAIAGDGAFQMVMQDFATAV
                     QYDLPLTVFVLNNKQLAFIKYEQQAAGELEYAVDFSDMDHAKFAEAAGGKGYTIKSAS
                     EVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDKYLDLD
                     QIPPISVAAKRFL"
     gene            complement(6399..7088)
                     /locus_tag="P6L01_000176"
     CDS             complement(6399..7088)
                     /locus_tag="P6L01_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDP3029278.1"
                     /translation="MNDYVQALLMILLTVVLYYFAKRLQQKYPNPFLNPALIASLGII
                     FVLLIFGISYNGYMKGGSWINHILNATVVCLAYPLYKNREKIKDNVSIIFASVLTGVM
                     LNFMLVFLTLKAFGYSKDVIVTLLPRSITAAVGIEVSHELGGTDTMTVLFIITTGLIG
                     SILGSMLLRFGRFESSIAKGLTYGNASHAFGTAKALEMDIESGAFSSIGMILTAVISS
                     VLIPVLILLFY"
     gene            complement(7081..7476)
                     /gene="cidA"
                     /locus_tag="P6L01_000177"
     CDS             complement(7081..7476)
                     /gene="cidA"
                     /locus_tag="P6L01_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like murein hydrolase modulator CidA"
                     /protein_id="HDP3029279.1"
                     /translation="MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFL
                     FYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIG
                     TCIVALSSGYIAEKMSVKHKHRKGVDAYE"
     gene            complement(7689..8567)
                     /locus_tag="P6L01_000178"
     CDS             complement(7689..8567)
                     /locus_tag="P6L01_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDP3029280.1"
                     /translation="MDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMG
                     TPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMN
                     MKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTL
                     DKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTV
                     AQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQL
                     SHATTKWIEFLKDRLG"
     gene            8734..8952
                     /locus_tag="P6L01_000179"
     CDS             8734..8952
                     /locus_tag="P6L01_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HDP3029281.1"
                     /translation="MSFYEFMQSFLGDDTPLGELVDWINQDSNFPREVKSHSEILSYF
                     RTNPCPESISVPVIKRALSVFNQFTNVQ"
     gene            9342..9773
                     /locus_tag="P6L01_000180"
     CDS             9342..9773
                     /locus_tag="P6L01_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDP3029282.1"
                     /translation="MEYKKILIRLLIAFAVLFSADFTYQSVEQTHQSHAAVNYYSKNQ
                     CTWWAFKRRAQVGKPVSNRWGNAKNWYYNARKSKYATGRTPRKFAVMQSTAGYYGHVA
                     VVEQVYKNGSIKVSEYNFYRPLKYNTRVLSKKAARNFNYIY"
     gene            complement(9912..11189)
                     /locus_tag="P6L01_000181"
     CDS             complement(9912..11189)
                     /locus_tag="P6L01_000181"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDP3029283.1"
                     /translation="MQSLDKNFRHLSRQQKLQQLVDKQWLSEDQFDILLNHPLIDEEV
                     ANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFK
                     TVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHKIADEAYPSIKARGGGYQRIAID
                     TFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNESPQSDILMSILSNHATASV
                     VKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLAT
                     GNDTRGAEASAHAYASRDGQYRGIATWRYDQKRQRLIGTIEVPMTLAIVGGGTKVLPI
                     AKASLELLNVDSAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGA
                     KGDEIAQVAEALKQEPRANTQVAERILQEIRQQ"
     gene            11444..12610
                     /locus_tag="P6L01_000182"
     CDS             11444..12610
                     /locus_tag="P6L01_000182"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDP3029284.1"
                     /translation="MTIGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAV
                     SPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFA
                     RCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAM
                     VIAHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAK
                     RQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNI
                     YTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKAL
                     LNNRTEVSVDAYETFFKRFDDVEFDEEQDAVHEDRHIFYLSNIENNVREYHRPE"
     gene            complement(12775..13296)
                     /locus_tag="P6L01_000183"
     CDS             complement(12775..13296)
                     /locus_tag="P6L01_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDP3029285.1"
                     /translation="MEYKSYYDSPVGRLELLSDGVSLTAVLFENQQGDGTREENTSLA
                     IFREATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQVPYGTLTTYGAIAKKVG
                     KVLDKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
                     MSKLYIPKHSTKP"
     gene            13705..15810
                     /gene="clpL"
                     /locus_tag="P6L01_000184"
     CDS             13705..15810
                     /gene="clpL"
                     /locus_tag="P6L01_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpL"
                     /protein_id="HDP3029286.1"
                     /translation="MNNGFFNSDFDSIFRRMMQDMQGSNQVGNKKYYINGKEVSPEEL
                     AQLTQQGSNQSAEQSAQAFQQAAQRQQGQQGGNGNYLEQIGRNLTQEARDGLLDPVIG
                     RDKEIQETAEVLSRRTKNNPILVGEAGVGKTAIVEGLAQAIVEGNVPAAIKDKEIISV
                     DISSLEAGTQYRGAFEENIQKLIEGVKSSQNAVLFFDEIHQIIGSGATGSDSGSKGLS
                     DILKPALSRGEISIIGATTQDEYRNNILKDAALTRRFNEVLVNEPSAKDTVEILKGIR
                     EKFEEHHQVKLPDDVLKACVDLSIQYIPQRLLPDKAIDVLDITAAHLSAQSPAVDKVE
                     TEKRISELENDKRKAVSAEEYKKADDIQNEIKSLQDKLENSNGEHTAVATVHDISDTI
                     QRLTGIPVSQMDDNDIERLKNISNRLRSKIIGQDQAVEMVSRAIRRNRAGFDDGNRPI
                     GSFLFVGPTGVGKTELAKQLAIDLFGNKDALIRLDMSEYSDTTAVSKMIGTTAGYVGY
                     DDNSNTLTEKVRRNPYSVILFDEIEKANPQILTLLLQVMDDGNLTDGQGNVINFKNTI
                     IICTSNAGFGNGNDAEEKDIMHEMKKFFRPEFLNRFNGIVEFLHLDKDALQDIVNLLL
                     DDVQVTLDKKGITMDVSQDAKDWLIEEGYDEELGARPLRRIVEQQVRDKITDYYLDHT
                     DVKHVDIDVEDNELVVKGK"
     gene            complement(15879..16049)
                     /locus_tag="P6L01_000185"
     CDS             complement(15879..16049)
                     /locus_tag="P6L01_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDP3029287.1"
                     /translation="MSVIINILIFLAIFGYALYTLVKFFKRSKQGKCGTCDINRDCCG
                     TEQHTANHFPGK"
     gene            complement(16062..18056)
                     /gene="feoB"
                     /locus_tag="P6L01_000186"
     CDS             complement(16062..18056)
                     /gene="feoB"
                     /locus_tag="P6L01_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDP3029288.1"
                     /translation="MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLK
                     ENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNVQLTVQLLE
                     LNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKYLLGEIKHLGE
                     GYQPHFKINYGEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEV
                     VNKLSSLRDQVAEQVGAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKIL
                     THKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFL
                     QDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKS
                     FIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFVGIFFKENQ
                     SLVVLSLYVLGIIMAFLVSTVLTKTILKNDNAIFIVELPTYRVPSIKTLWRSTWEKAK
                     GFVRKAGTFIFGGSVVIWLLSYVGPHGINVNINQSFLHMVGSFFGMLVQPLGFGTWQA
                     GATLVPGFLAKEVIVSSMAIIYSSGDAGLVNVIQNQFTPLSAYAFMIFILLYIPCVST
                     VAAIRKETYSWKWTALAVAYPLVTAYVLTFIFYQVGHLFV"
     gene            complement(18068..18295)
                     /locus_tag="P6L01_000187"
     CDS             complement(18068..18295)
                     /locus_tag="P6L01_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDP3029289.1"
                     /translation="MLNIKNGEMNKAYKIKRMDIANENMLYRLSAFGLTDDAIITIKQ
                     KCLFKGPCIIEVNGQQLSIRHCDACSIALEE"
     gene            complement(18511..21000)
                     /gene="farE"
                     /locus_tag="P6L01_000188"
     CDS             complement(18511..21000)
                     /gene="farE"
                     /locus_tag="P6L01_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux MMPL transporter FarE"
                     /protein_id="HDP3029290.1"
                     /translation="MAKFLYKMGTFIAKHKWSAVIAWIVIVAAILIPLATNAPKFDND
                     IKMTGLESLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPNITEDIKKTLEDIKK
                     DDKHIDKISDPYENKQISKDKTTAFADITYDVSQTSLKDGSRDNVKSHLKDLRDNHNV
                     QTELTGTGMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGII
                     SLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYIKGIGLAIGTAG
                     SAVVFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVIFAVFSALTLLPALISIFHKRI
                     KVNKLQSNFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPA
                     DSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDI
                     VTPPQMSKDNDYALMVVIPKQGPNAESTNDLVHDLRDYHKDAQDKYGFKTEISGQSVI
                     NIDMSKKLNEAIPLFATVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTLV
                     MQDGFMKGLFGIETTGPMLAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYS
                     IKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIP
                     ALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKVY
                     PQGATNVSKHQDVHGQDDAHSIVLDDKTMALYQEVKQQSASSLFLYDALIDYQNKHQL
                     NSKQQVTNIEQLNKNIEKLNQLLEKNLRNKS"
     gene            21145..21693
                     /gene="farR"
                     /locus_tag="P6L01_000189"
     CDS             21145..21693
                     /gene="farR"
                     /locus_tag="P6L01_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux pump transcriptional regulator
                     FarR"
                     /protein_id="HDP3029291.1"
                     /translation="MKETDLRVIKTKKALSSSLLQLLEQQLFQTITVNQICDNALVHR
                     TTFYKHFYDKYDLLEYLFNQLTKDYFARDISDRLNHPFQTMSDTINNKEDLREIAEFQ
                     EEDAEFNKVLKNVCIKIMHNDIKNNRDRIDIDSDIPDNLIFYIYDSLIEGFIHWIKDE
                     KIDWPGEDIDNIFHRLINIKIK"
     gene            complement(21770..21937)
                     /locus_tag="P6L01_000190"
     CDS             complement(21770..21937)
                     /locus_tag="P6L01_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029292.1"
                     /translation="MRYVISIIMGIVLAIWSYKQLSQSHLDSGFIFFFIVYVLCISYF
                     NNDKHDKNKKR"
     gene            complement(22049..23593)
                     /gene="pruA"
                     /locus_tag="P6L01_000191"
     CDS             complement(22049..23593)
                     /gene="pruA"
                     /locus_tag="P6L01_000191"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HDP3029293.1"
                     /translation="MVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGE
                     KIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELML
                     RVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDR
                     EGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLM
                     EILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEG
                     QNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYD
                     EVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDS
                     KGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITN
                     NREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQK
                     VVSEMF"
     gene            complement(23783..24382)
                     /locus_tag="P6L01_000192"
     CDS             complement(23783..24382)
                     /locus_tag="P6L01_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDP3029294.1"
                     /translation="MTEKEKMLAEKWYDANFDQDLINERARAKDICFELNHTKPSDTN
                     KRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVF
                     IGPNCGFYTATHPLNFYHRNEGYEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAG
                     SVVTKDIPPHSLAVGNPCKVVRKIDNDLPSETLNDETIK"
     gene            24621..24787
                     /locus_tag="P6L01_000193"
                     /pseudo
     CDS             24621..24787
                     /locus_tag="P6L01_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501327.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25027..27435
                     /locus_tag="P6L01_000194"
     CDS             25027..27435
                     /locus_tag="P6L01_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDP3029295.1"
                     /translation="MANTKKTTLDITGMTCAACSNRIEKKLNKLDDVNAQVNLTTEKA
                     TVEYNPDQHDVQEFINTIQHLGYGVTVETVELDITGMTCAACSSRIEKVLNKMNGVQN
                     ATVNLTTEQAKVDYYPEETDADKLVTRIQKLGYDASIKDNNKDQTSRKAEALQHKLIK
                     LIISAVLSLPLLMLMFVHLFNMHIPALFTNPWFQFILATPVQFIIGWQFYVGAYKNLR
                     NGGANMDVLVAVGTSAAYFYSIYEMVRWLNGSTTQPHLYFETSAVLLTLILFGKYLEA
                     RAKSQTTNALGELLSLQAKEARILKDGNEVMIPLNEVHVGDTLIVKPGEKIPVDGKII
                     KGMTAIDESMLTGESIPVEKNVDDTVIGSTMNKNGTITMTATKVGGDTALANIIKVVE
                     EAQSSKAPIQRLADIISGYFVPIVVGIALLIFIVWITLVTPGTFEPALVASISVLVIA
                     CPCALGLATPTSIMVGTGRAAENGILFKGGEFVERTHQIDTIVLDKTGTITNGRPVVT
                     DYHGDNQTLQLLATAEKDSEHPLAEAIVNYAKEKQLTLTETTTFKAVPGHGIEATIDH
                     HHILVGNRKLMADNDISLPKHISDDLTHYERDGKTAMLIAVNYSLTGIIAVADTVKDH
                     AKDAIKQLHDMGIEVAMLTGDNKNTAQAIAKQVGIDTVIADILPEEKAAQIAKLQQQG
                     KKVAMVGDGVNDAPALVKADIGIAIGTGTEVAIEAADITILGGDLMLIPKAIYASKAT
                     IRNIRQNLFWAFGYNIAGIPIAALGLLAPWVAGAAMALSSVSVVTNALRLKKMRLEPR
                     RKDA"
     gene            27846..28052
                     /gene="copZ"
                     /locus_tag="P6L01_000195"
     CDS             27846..28052
                     /gene="copZ"
                     /locus_tag="P6L01_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDP3029296.1"
                     /translation="MSQEILNVEGMSCGHCKSAVESALNNIDGVTSADVNLENGQVSV
                     QYDDSKVAVSQMKDAIEDQGYDVV"
     gene            complement(28143..29129)
                     /locus_tag="P6L01_000196"
     CDS             complement(28143..29129)
                     /locus_tag="P6L01_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDP3029297.1"
                     /translation="MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVA
                     GYDGLSLSQQIPLSEHVYKRLNELGIKQIAQRSAGFDTYDLELANKYNLIVSNVPSYS
                     PNSIAEFAVNQAINVVRHFNQIQTKVREHDFRWEPTILSKSIKDLKVAVIGTGRIGRV
                     VADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLHVPATKYNHYLF
                     NAELFKHFKKGAVFVNCARGSLVDTKALLDALDNGVIKGAALDTYEFERKLFPSDQRG
                     KTPNDPLLESLIDREDVILTPHIAFYTEAAVKNLIVDALDVLQTGDARLRVN"
     gene            complement(29152..30306)
                     /locus_tag="P6L01_000197"
     CDS             complement(29152..30306)
                     /locus_tag="P6L01_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDP3029298.1"
                     /translation="MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQG
                     IIDHFQKALTIPENQKYGAFHGKEAFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGL
                     VAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSTVDSQIID
                     KTKLIYLTYPNNPTGSTATKEVFDEAIAKFKGTDTKIVHDFAYGAFGFDAKNPSILAS
                     ENGKDVAIEIYSLSKGYNMSGFRVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIY
                     ALNHYDDFLEEQSNVFKTRRDRFEAMLAKADLPFVHAKGGIYVWLETPPGYDSEQFEQ
                     FLVQEKSILVAPGKPFGENGNRYVRISLALDDQKLDEAAIRLTELAYLYE"
     gene            complement(30734..32242)
                     /locus_tag="P6L01_000198"
     CDS             complement(30734..32242)
                     /locus_tag="P6L01_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDP3029299.1"
                     /translation="MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKK
                     DGFTFDMGPTIVMMPDVYKDVFTACGKNYEDYIELRQLRYIYDVYFDHDDRITVPTDL
                     AELQQMLESIEPGSTHGFMSFLTDVYKKYEIARRYFLERTYRKPSDFYNMTSLVQGAK
                     LKTLNHADQLIEHYIDNEKIQKLLAFQTLYIGIDPKRGPSLYSIIPMIEMMFGVHFIK
                     GGMYGMAQGLAQLNKDLGVNIELNAEIEQIIIDPKFKRADAIKVNGDIRKFDKILCTA
                     DFPSVAESLMPDFAPIKKYPPHKIADLDYSCSAFLMYIGIDIDVTDQVRLHNVIFSDD
                     FRGNIEEIFEGRLSYDPSIYVYVPAVADKSLAPEGKTGIYVLMPTPELKTGSGIDWSD
                     EALTQQIKEIIYRKLATIEVFEDIKSHIVSETIFTPNDFEQTYHAKFGSAFGLMPTLA
                     QSNYYRPQNVSRDYKDLYFAGASTHPGAGVPIVLTSAKITVDEMIKDIERGV"
     gene            complement(32254..33117)
                     /locus_tag="P6L01_000199"
     CDS             complement(32254..33117)
                     /locus_tag="P6L01_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HDP3029300.1"
                     /translation="MTMMDMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVC
                     RKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEHHHFQSDRRIMMALQHVAQHKNIAFQ
                     SFYNLIDTVYKDQHFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRL
                     GESLQLINILRDVGEDFDNERVYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAI
                     AEKDFQDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVDKRKKAKL
                     FHEINSKYHRI"
     gene            complement(33154..34281)
                     /gene="crtQ"
                     /locus_tag="P6L01_000200"
     CDS             complement(33154..34281)
                     /gene="crtQ"
                     /locus_tag="P6L01_000200"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,4'-diaponeurosporenoate glycosyltransferase"
                     /protein_id="HDP3029301.1"
                     /translation="MKWLSRILTVIVTMSMACGALIFNRRHQLKTKTLNFNHKALTII
                     IPARNEEKRIGHLLHSIIQQQVPVDVIVMNDGSTDETARVARSYGATVVDVVDDTDGK
                     WYGKSHACYQGVTHACTNRIAFVDADVTFLRKDAVETLINQYQLQGEKGLLSVQPYHI
                     TKRFYEGFSAIFNLMTVVGMNVFSTLDDGRTNQHAFGPVTLTNKEDYYATGGHKSANH
                     HIIEGFALGSAYTSQSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGAGGTKPK
                     IMTAIVLWLFGSIASILGLCLSLKYRQMSVRKMVALYLSYTTQFIYLHRRVGQFSNLL
                     MVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYSISKEQ"
     gene            complement(34287..35780)
                     /locus_tag="P6L01_000201"
     CDS             complement(34287..35780)
                     /locus_tag="P6L01_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDP3029302.1"
                     /translation="MTKHIIVIGGGLGGISAAIRMAQSGYSVSLYEQNTHIGGKVNRH
                     ESDGFGFDLGPSILTMPYIFEKLFEYSKKQMSDYVTIKRLPHQWRSFFPDGTTIDLYE
                     GIKETGQHNAILSKQDIEELQNYLNYTRRIDRITEKGYFNYGLDTLSQIIKFHGPLNA
                     LINYDYVHTMQQAIDKRISNPYLRQMLGYFIKYVGSSSYDAPAVLSMLFHMQQEQGLW
                     YVEGGIHHLANALEKLAREEGVTIHTGARVDNIKTYQRRVTGVRLDTGEFVKADYIIS
                     NMEVIPTYKYLIHLDTQRLNKLEREFEPASSGYVMHLGVACQYPQLAHHNFFFTENAY
                     LNYQQVFHEKVLPDDPTIYLVNTNKTDHTQAPVGYENIKVLPHIPYIQDQPFTTEDYA
                     KFRDKILDKLEKMGLTDLRKHIIYEDVWTPEDIEKNYRSNRGAIYGVVADKKKNKGFK
                     FPKESQYFENLYFVGGSVNPGGGMPMVTLSGQQVADKINAREAKNRK"
     gene            complement(35773..36261)
                     /gene="crtO"
                     /locus_tag="P6L01_000202"
     CDS             complement(35773..36261)
                     /gene="crtO"
                     /locus_tag="P6L01_000202"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase"
                     /protein_id="HDP3029303.1"
                     /translation="MKKYIKTAFFCSMYWLIVQLNIANLGTRIPDKYFRQKYIIFKSF
                     NFEKHGKFWNKWFYVRKWKHKILDGHQLNQNIYDQRHLMTINTDEIEKMIIETKRAEL
                     IHWISILPVIIFNKGSRLVKYINIFYAMIANVPIIIVQRYNRPRLTQLLRILKRRGER
                     HD"
     gene            complement(36436..37203)
                     /locus_tag="P6L01_000203"
     CDS             complement(36436..37203)
                     /locus_tag="P6L01_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDP3029304.1"
                     /translation="MKKIVTATIATAGLATIAFAGHDAQAAEQNNNGYNSNDAQSYSY
                     TYTIDAQGNYHYTWTGNWNPSQLTQNNTYYYNNYNTYSYNNASYNNYYNHSYQYNNYT
                     NNSQTATNNYYTGGSGASYSTTSNNVHVTTTAAPSSNGRSISNGYASGSNLYTSGQCT
                     YYVFDRVGGKIGSTWGNASNWANAAASSGYTVNNTPKVGAIMQTTQGYYGHVAYVEGV
                     NSNGSVRVSEMNYGHGAGVVTSRTISANQAGSYNFIH"
BASE COUNT        11867 a         7175 c         5753 g        12486 t
ORIGIN      
        1 tcggcgctct ggtatcggaa atactggata aatatggaat tggtaatatc caggtataaa
       61 gtcatgttcg ataccttttg cactcaacaa ttgcgataag ttcaaagcat ctgggtacag
      121 tacttctttt gtgccaacag ttaatgtaat acgacctaaa ccatctaagt caccattaat
      181 tggtgatact ttgtagttat ctaaaggtgt gtcccctgcc cattgttcag ctaagaacac
      241 actgccatcc aaacctacca ttgggtcttt ctttaagtag tcaggaattt caggatgctg
      301 catcgttgca tctaaaactg gtgaaattaa tacaatatgt cctggttgca caatatgttt
      361 ttcttttaac aattgagcaa atgataaagc aatttgaccg cccgcagagt cacccattac
      421 aacgatttgt ttagaatctg ctacttgatt caataaatca tggtataact tttcaaaaag
      481 cacatacgtc gcttgataat cttgatgcgg aatcttcgga tatactggca tgatgacttt
      541 agcattgagt gtttctgcaa gttcatcaat aaattcgaaa tgaattttga gtgggtcttg
      601 gaaccatgcg cctccatgtg cgtataatac aacgcgttga tgcttatctt gtttatcatt
      661 aacgttataa acggttgaac cgtagtgctt atccactgtt actggcatct taaactgata
      721 attggtgccc tcatattcac ctgctgtctg tggcgccatt ttttctaatg ctttcatcgc
      781 ttcttcttta tacataaaac gacgcttagc tctagataat cgtatacctt gttctaacat
      841 ataacttttc acactgcgtt tttctttaat tctaatatgt gcgtatgctg ctactaaaaa
      901 tccaaacgca agtgtagacc attttttacg catttgtcgt cactcctctt tgatgatttc
      961 tactatagtt ctactgtacc atgctgattc ttattactac caatatttac tgggaaataa
     1021 gttatgatgt ctacataatc ttagcaatgc taatcatttg cgatattcat gctcatcgac
     1081 atcgttattc acactatcat tcatttgctt aagatgatgt tcaaaacgtt gcatatcgat
     1141 aaaacgccct tgacgatgca tttcttttcc attcataaaa attaaatcca caggtacagt
     1201 gaaaatattt aattctccag caatagcttc aacttgactt tgattaatca cagctaatgg
     1261 cacattggga tatgattgca tcaagtcttc aatttgtggt aaaacggcat gacacacgct
     1321 acactgatcg cgcatgacat gtacaactgc taacggatat tgatgaataa attcacggaa
     1381 tgcttcgaca ctatttaatt gcttaaccat ttcgaacacc ttcttacttt ttatatttta
     1441 aaatgaagtc attattttgt tgctaaaatg tcttgaaaca cttgtggcac agaggttatc
     1501 ttcttatcac tcaatctgta aaaaccgcct gtctgtgtac caatacaatg cacgcaatgt
     1561 gcggacacaa tttcaaactg aaacacccat ttcatcttgc ttgctttaac taagtaaata
     1621 cgacctactg gtttgtcatg tattgtgaca ggtactttat actctgcttc cgttttcata
     1681 agtataggtg aaatgccttg attgatcatt gttgagtaat tcatgtgctg attaataaac
     1741 gctgttctca aatcttcgaa ccatctaata taaacgatat tactaacgac acccatcgca
     1801 tcaatgtcgt aaccattcac ttcgatttct ttctctgcaa ctaatagacc ttctgttgtc
     1861 ataatgacaa atctccttat cttacaaata gatttaaaat catgttaatt tatttttatt
     1921 actttcatga taaaaataca agcaattgaa ctttattacg gaaaatcaaa cttttcaatg
     1981 agactattaa tttggctagt cgcgaagatg catttttcta aaatgtgaca aaaacataag
     2041 caatgttttc tatatctaac actaatattt aacttattat catcaacaca cctgctatac
     2101 atcattgcgc actaaaaagg cagtagcttc attttaagct actgcctttt gtaacgtatt
     2161 ctctttcaac tcgctacttc gcatacgcac tacgaatgac taaaagttca ttcatttgat
     2221 atgctgtttc atttttaatc attatttgac tgtcataatt agcttacctg gatcagcacc
     2281 ttgtacatct tcaatgacaa gttctttatt ttcaagattt gtaataatca ttggtgtaac
     2341 gatgcttggt gcgtgtgctt tcaagtatgc taaattcact ttcattaatg gttggccttg
     2401 tgttactttt tcatcaacgt taatcagact ttcgaatcct tcaccattca gtttcactgt
     2461 atcgatacca atatgaataa gtacttcgac gccactttca gattctaatc ctatcgcatg
     2521 tttcgtaggg aagattgttt tcactgtacc atcaaacggt gctacaattt cacctttttc
     2581 agggataaag ccaataccat cacccatcat tttaccagcg aatacttgat caggtacttc
     2641 tgataatgga atgatttgac cgacacctgg tgcatagatg tctgttgttc caagtgcttc
     2701 tacgtgaact ggttcatctg acatttcttc agtcactgtc gtttcacttg gtttcgtaat
     2761 ttcaccactc ataatcttgg ccatatcatg tttaatttga tctgattttg gaccaaagat
     2821 agcttgcata ttgtttccaa cttctaatac acctgatgcg cctaaagctt taatacctgc
     2881 tacatctact tttgatttat caaccacttc tacgcgcaga cgtgtaatac atgcatctaa
     2941 atgtttaatg ttttcttttc cacccattgc atctaagaca tcaaatggta attttgcgac
     3001 actagagtta cgaatttcag tttcttcatc ttcacgacct ggtgttttca atttaaactt
     3061 acgaattgca aagtcgaata agaagtaata cacgatagca tatacaatac cgactggaat
     3121 aactaataat gcgtgtgaac gatcccagtt taataaacca tataaaatat aatctatgaa
     3181 accacctgag aatgtcatac caattttaac gcctaataaa tgcattacta agaatgatgt
     3241 accagctaat aatacgtgaa ttccataaag tactggtgct acaaataaga atgaaaattc
     3301 taatggctca gtgataccag ttaaaaatgc agttaatcct gctgataaca ttaaaccacc
     3361 cacgactttt ttgcgttctg gtcgtgcatt tttataaata gcaaatgccg ccgctggtaa
     3421 accaaacatc ataaatggat atttaccagt agtaaatgca ccagcagtaa atggtacgcc
     3481 atctttcaat tgtgccatcc aaatacgttg gtcaccacga actaattcac ctgcgtgatt
     3541 tgtataactt ccgaattcaa accagaacgg tgaatagaaa atatgatgta aaccaaatgg
     3601 aattaatgag cgttcaataa taccgaatat aaacgttgtt aatgttaaat ttttatttaa
     3661 taagaaattc gataaactat ttaatccatc ttgaattggt ggccacgcaa agcttaaaag
     3721 cacacctgtt gcgattgcta cgaccgatgt cacaatcggt acaaatcgtt tacctgcaaa
     3781 gaatcctaaa aatggtggta gtgtaatatt ataaaatttg ttgtaacacc atgcggctaa
     3841 agcacccatg ataataccac caaacacacc cgtttgtaac gttggaatac ctaatactaa
     3901 agcatgtgct ggttctttcg ctgcttgact taattctttt gcccctttgg catatgagaa
     3961 aatgtcatca attgtaatgt gcaacacttt ccccattgtt gcattcataa ttaagtaacc
     4021 tactagcgct gctaatgctg caacaccgtc tcctcctgct aatccaagtg ctgtaccaac
     4081 tgcaaataat aatggcaagt tatcaaatac aacttgtcct gctgcttcca tgaccgacga
     4141 aatcattaca atgatatcgt tttttaacca tggtgcaatt tctactaatt gttcgttgtg
     4201 cattgcgtta ccaaacgcta ataaaatacc agctgctggt aaaatcgcaa caggtaacat
     4261 taatgcttta ccgatacgtt gcaattgtcc aaacaatttc ttaaacatat aaacatcccc
     4321 tatctcaatt tccgcttcca tgtacaaaaa aagcaggcat gaacaaacaa gactattctc
     4381 atagtacggt tgtttgctca tgcctgcatt accagtaaca cgcaagtgat atgtaattca
     4441 tagctattat acatatttgt aaaagcgttt tcaacaactt tttcacaatt ttatcatcaa
     4501 ttcagtacga tcatttgtga taaataaaca aaacggcaat tcccattttg ttgaaataga
     4561 aattaccgtt tttatagttc aattcaattg tgataacctt tcaatcagtt ataagaaacg
     4621 ttttgctgca actgaaatag gtggaatttg atctaaatct aaatatttat cttcagtaat
     4681 agatctaaat gcccatttac cataaccaag cgcttcttca tttacaattt tacctggtaa
     4741 tggcgcagca ttaggatcaa catatacatc tacaatcgtt ggtacatctt gtgctaatgc
     4801 ctcttcgact atagcatcta cttcgctagc actcttaatt gtataacctt taccacctgc
     4861 tgcctcagca aattttgcat gatccatatc agaaaaatca actgcatatt ctaattcacc
     4921 agctgcttgt tgttcatatt taataaatgc taactgtttg ttattaagta caaatacagt
     4981 taaaggtaaa tcatattgta ctgctgtagc gaagtcttgc attaccattt ggaatgcacc
     5041 gtcaccagca atggcaatcg cttgtctatt tggatatgca atttttgatg ccatagctcc
     5101 tggaagaccg caacccattg tacctaacca acttgaaatg atgaacttgt tgttcacacc
     5161 aagatttaaa taacgtgtag accaaactgt tgcagtacca acatctgctg aaattactgc
     5221 atcatcttta ataaatttgt taattgatgc cattaaacgt tctggacgta atggtttgct
     5281 attattattt ttatcttgtt ccatccattt atcccaaacc gctttacgtt ctaacgtttt
     5341 gtttaaaaat ggtctttccg caacatgttt aatattttca gttaactgat gcaatgcaat
     5401 tttactgtct ccaacaattc ctacattaat attaaaacga tgtccgatat ttttaggatt
     5461 tgtgtcgatt tggattgctt taatattttt cttaggtaag taatccacat atggatagtt
     5521 tgtaccaacc ataattaata aatccgcttc ctgcattgtt tgataagatg ttttggtacc
     5581 aattttacct aagttaccga tactatatgg atgatcatcc ggcaagattg ttttagctgg
     5641 taatgaatga atgacaggaa ttttagccat ttcaataaat tcacgtagct catctttcgc
     5701 atgttttgca cctacaccaa ttaacatgac aggcttttta cttttattaa ttagtttaac
     5761 cgcttttttg atgtctttat attttggtga tacaactgtt ggtcttgatg tatctactgg
     5821 tttattcgtt gtatctttaa ttttttcagt taataagtcg ttaggacaaa taacaacagc
     5881 gacacctttt tgttcatatg ccgtacgaat tgcttcgtta acgatttcaa acacattgtc
     5941 acctttttca atttggtgat tataaacggc tacatcttca cataattttt gtaaatttgt
     6001 ttcttgaaat gctttcgttc caagtgctgt actattcgtt tgtccagata atattaattg
     6061 cggtacatta tccattttgg catcgtacat accatttaat aaatgaatta aaccagggcc
     6121 accgatactt aatgccacac cgattttacc agttaatttt gtgtaaccag cagccgctaa
     6181 gcttgctact tcttcatgac gtacatgata aaatttaaat tgatctctca ctgtacgtaa
     6241 actatcgaca actgcgtcga ttgagtctcc tggaatacca tacaagtgat ctatatccca
     6301 tgcttgtaat gctttaacta atgcttcatt tgcttttatt tttgccatta ttactaatag
     6361 cctccctttc ttagtgtcta tcattttaaa tatctaaatt aatagaataa taaaattaga
     6421 acaggtatta acactgaact aataactgca gttaaaatca tcccaattga actaaatgca
     6481 ccggattcaa tatccatttc tagtgcttta gctgtgccaa atgcatgtga cgcattccca
     6541 tacgttaaac ctttggcgat agaagattca aatcttccaa atcttaataa catcgaacct
     6601 aaaatactac cgattaaacc cgttgtgata ataaaaagta cggtcatcgt atctgtacca
     6661 cctagttcat gtgacacttc gatacctact gcggctgtta tagatcgggg caataacgtt
     6721 acaatgacgt ctttagaata gccaaatgct ttaagtgtta agaacactaa catgaaattc
     6781 agcatgacgc cagttaatac acttgcaaaa atgatagaga cattgtcttt aattttctct
     6841 ctatttttat aaagtgggta cgctaaacat acgaccgttg cgtttaaaat atggttgatc
     6901 caactgccac ctttcatata cccgttataa ctaattccaa agataagtaa gacaaaaata
     6961 attcctaaag atgcaattaa tgctggattc aaaaatgggt tcggatattt ttgttgtaac
     7021 cttttagcga aataatataa aacgacagtc aacaaaatca ttaataaggc ttgcacgtaa
     7081 tcattcataa gcgtctacac ctttacgatg tttatgttta acagacattt tttcagcaat
     7141 ataacctgaa gataatgcaa cgatacatgt tcctataata atgactgcga aaaagagtat
     7201 ataatttagc gtaatttcgg aagcaacatc cataatacct acaactgacg gtatgaagaa
     7261 aaagaccatc gtctttaata aaaagtttgc accgtcttct acccaagtta gcggtacaat
     7321 cttaaattgt aatagtaaat aaaataaaaa tagaccaaca atactgccgg ctaagggaag
     7381 atgaaaaatc ttttgaattt ctgtgccaat ataagtaatc acaatgatga ttcctagttg
     7441 tagtagtagt ttgattatta attggacttt gtgcatggcg ccatcccttt ctaaatatgt
     7501 ctaaaatgtt acaataacta ttataaagat ggcgtgcaca atataaaaat actctttttt
     7561 tatagtttgt attactattt tctatggaaa cgctctctca atttcacttc attttcacaa
     7621 aaaaacactc atcatttcaa tgacccacgt aatgatgcgc cttaaataat gagtgcttta
     7681 ttaatatgtt agcctaaacg atctttcaaa aattctatcc atttcgttgt ggcatgactt
     7741 aattgtttat ccttcttcca aacaacacct aattcccaat gtacatgcgc atcttcaatg
     7801 cgtagcagct tcacatctcc atttagttgc tctgaaattg atgttggtaa aatactaata
     7861 cctaattcat tcgtaactaa atcttctata acatgccatt gtgaaatttg cgctacagta
     7921 ttcggaacaa agccaacgtt tttagcattt tcaataattt tatcattcaa gtaaaagtct
     7981 ttattaaata aaatgaagtc ttcacctgct aaatcttcga gtttaacagt ttcatatttt
     8041 gcgagtcgat gctctctgct cacgataagt cgcaaatctt ccttatctag ggtagtataa
     8101 tcgaaaatat gatgatcgac tggcaaagtg gtcacgccta tatctacttc atcattgata
     8161 atttgctgct caatcgtttt gccgccattt tctattaaat tatatgtaac atttggatat
     8221 tgctgatgga atgcaccaag aatattgata agaatcttca tattcatgac tgccgacatg
     8281 cccatgttta tatgccctgt ttccagtcca ttcaagcgtt ccatttcaga tggtaaataa
     8341 tcatacagtg caacaatttc tttacttttc tcataaaaaa tttgacctgc atcggtaagt
     8401 attaaatgtc ttttacttct atcaaataag ggcgtgccca tttcattttc aatatcttta
     8461 attgctttac taattgtagg ttgtgcaata tataatgatt tggaggcatt agtcatgcct
     8521 ccttgcttaa cgacttcaat aaaatatttc atatgtttga tatccactaa catcatccta
     8581 actatttcaa cacgtttatt tcattataca caacttctta aacattcaat aaatttagat
     8641 ttataaaatt gctttataaa aagttttatg taaaatacac attcttttat ggtattatat
     8701 taatggctca agtttgttat tcgaggtgag gtattgagct tttacgaatt tatgcaaagt
     8761 tttcttggtg atgacacacc attaggtgaa ttggttgatt ggattaatca agatagcaat
     8821 ttccctagag aagtgaagag ccatagtgaa atattgtcat attttcgaac aaatccatgt
     8881 cctgaaagca tctcagtacc tgtaattaaa cgggcactat cagttttcaa ccaattcaca
     8941 aatgtacaat gatacatgaa tacatggagt cacatcatct catgttagcg ctctgattgc
     9001 agggcgctat ttttaatgtt atcgctacat ttagtatatt gtgattgcta ttttctttca
     9061 gttatagaac cacgtacctt ttcttttaaa tcattttata ttttcccact aatatccgct
     9121 gttaatcaat tctgacatcc ttgtatcact atgacaatta attgttaaat acatgaattt
     9181 ctacatttta tgaaaaaatc catttttatt acaattcaac actttatatg acaacttcat
     9241 tacagttact tttattgttg attgcttgca ttgttttcta aaaaaatttt gttatcataa
     9301 ttaacgttga ataaagaaaa aaattaactt gggagataaa aatggaatat aaaaagatac
     9361 taattcgttt attaattgct tttgcagtac ttttctcagc agatttcact tatcaatccg
     9421 tcgaacaaac gcatcaatcg catgccgcag ttaattatta tagtaaaaac caatgtacat
     9481 ggtgggcatt taaacgtcgc gcacaagtcg gcaaacctgt ttctaataga tggggcaatg
     9541 ctaaaaattg gtattacaat gcacgtaaat caaaatatgc gactggtcgt acaccaagaa
     9601 aatttgctgt catgcaatca actgcaggat attatggaca tgtcgcagtt gttgaacaag
     9661 tatataaaaa cggtagtatt aaagtttcag aatacaactt ttatcgccca ttaaaataca
     9721 atacacgtgt actaagcaaa aaggcagcac gtaactttaa ctatatttac taatcaaaaa
     9781 acttctatca cgaacgcttc aatttcctgt atgcgtgtga tagaagtttt tattttttgt
     9841 tcatattaat ttatctaagc gctacgatgg actgacttaa acttttcttt aaaatcgcta
     9901 ttcgccattt actattgttg tctaatttct tgtaaaatgc gttcagctac ttgtgtattc
     9961 gcacggggtt cttgcttcaa tgcttcagct acttgcgcaa tttcatcacc ttttgcacct
    10021 acaacaatag ctaaagattt atattgcaag ctcatatggc cttgctggat accttcggaa
    10081 acgagcgcgc gacatgctgc aaagttctgt gctaaaccaa cggcagcaac tacatgacct
    10141 aattcttgtg ctgaatctac atttagcaat tctaaagaag ctttagcaat tggtaatact
    10201 tttgtaccac cgccaacgat tgccaatgtc ataggcactt ctattgtacc aattaaacgt
    10261 tgacgttttt gatcgtatct ccatgttgca ataccacgat actgtccgtc acgactcgcg
    10321 tatgcatgcg cacttgcttc tgcaccacgc gtatcatttc ctgttgctaa aacaacggca
    10381 tgtatgccat tcataacacc tttattatgt gttgcagcac gatgaatatc aacttgtgcc
    10441 aatacagaag cacgttccat tcgtttggca acctcttctc cagttctctc gccccttgct
    10501 aaatctttaa cgtcaatttc gccttgaact ttaacaacgg acgctgttgc atgattggat
    10561 aaaatactca ttaaaatgtc gctttgtgga gattcatttt ttaaaaatgc agttatggcc
    10621 tctaaaatcg tattaagcat attagcgccc atagcatctt tcgtatcaac aaatactttt
    10681 aaagatagta actgttgctc aggaaatgta tcaatagcta tacgttggta accaccacca
    10741 cgcgctttaa tagaaggata tgcctcatcc gcaattttat gaatttgctt ttctaaagct
    10801 ttaatgtctg ctgataattt ttcagtatcg tcaacgccat caaagacgat ttgacctatc
    10861 ataatacgtt cagaagatac cgttttaaat ccgccagtct gattcactag ctttgcacca
    10921 taactagctg cagcgacaac tgaaggctct tccaccatca taggtacaac atatgcctta
    10981 tcgtccacaa tgatattcgg taataatcca acgggtaatg caccttgcgc gatgacattt
    11041 tcaattaaac tatttgctac ttcctcatca attaatggat gattcaataa aatgtcgaat
    11101 tgatcttctg ataaccattg cttatctacc aattgttgta acttttgttg acgagataaa
    11161 tgtcggaaat tcttatctaa actttgcatg gacatactcc ttttacttca cataattttt
    11221 taacatttca atcactacta tttttaccat aaaataacgt tattcgtctt aaaattcaat
    11281 tgaataatta ttgttttaac tataaaataa agcaaggtaa attaaaacgc ttacaagaaa
    11341 cgtcaaatca tttttaaatt tagtgtattt ctttgtataa gataagcata ttctgatatg
    11401 atacaagtgt tgcttttata aatttgaaag gatgtaaaac cttatgacaa taggtatcga
    11461 caaaataaac ttttacgttc caaagtacta tgtagacatg gctaaattag cagaagcacg
    11521 ccaagtagac ccaaacaaat ttttaattgg aattggtcaa actgaaatgg ctgttagtcc
    11581 tgtaaaccaa gacatcgttt caatgggcgc taacgctgct aaggacatta taacagacga
    11641 agataaaaag aaaattggta tggtaattgt ggcaactgaa tcagcagttg atgctgctaa
    11701 agcagccgct gttcaaattc acaacttatt aggtattcaa ccttttgcac gttgctttga
    11761 aatgaaagaa gcttgttatg ctgcaacacc agcaattcaa ttagctaaag attatttagc
    11821 aactagaccg aatgaaaaag tattagttat tgctacagat acagcacgtt atggattgaa
    11881 ttcaggcggc gagccaacac aaggtgctgg cgcagttgcg atggttattg cacataatcc
    11941 aagcattttg gcattaaatg aagatgctgt tgcttacact gaagacgttt atgatttctg
    12001 gcgtccaact ggacataaat atccattagt tgatggtgca ttatctaaag atgcttatat
    12061 ccgctcattc caacaaagct ggaatgaata cgcaaaacgt caaggtaagt cgctagctga
    12121 cttcgcatct ctatgcttcc atgttccatt tacaaaaatg ggtaaaaagg cattagagtc
    12181 aatcattgat aacgctgatg aaacaactca agagcgttta cgttcaggat atgaagatgc
    12241 tgtagattat aaccgttatg tcggtaatat ttatactgga tcattatatt taagcctaat
    12301 atcattactt gaaaatcgtg atttacaagc tggtgaaaca atcggtttat tcagttatgg
    12361 ctcaggttca gttggtgaat tttatagtgc gacattagtt gaaggctaca aagatcattt
    12421 agatcaagct gcacataaag cattattaaa taaccgtact gaagtatctg ttgatgcata
    12481 tgaaacattc ttcaaacgtt ttgatgacgt tgaatttgac gaagaacaag atgctgttca
    12541 tgaagatcgt catattttct acttatcaaa tattgaaaat aacgttcgcg aatatcacag
    12601 accagagtaa tctgtgtatt tgaaagacat ttaaatataa cctaaaaaca gcagtaagac
    12661 agcttctcat tgaaatcgtc ttactgctgt tctctattta taacacttcg tattgaatga
    12721 attcattatg cctatttgac acattattga aatttttaat atacccctta tacgttacgg
    12781 ctttgtactg tgtttaggta tataaagctt tgacatatcg atattctcca actctaacag
    12841 cttaatttta ttattaatcg ttccaccgaa tcctgttaag ctacccgttt taccgacaac
    12901 acgatgacat ggcacgataa ttgataatgg attacttccg actgcacctc caaccgcttg
    12961 ggctgacatt tttggcttat caagcacctt acctactttt ttggcaatag caccatacgt
    13021 tgttagagtc ccataaggaa cctgtcttaa ttcattccaa acacactgtt gaaaatgact
    13081 acctgttggc tttaaaggta ttgtgatttc aggattgtca cctttaaaat acgcgtctaa
    13141 ccactgtgtc gcctctctaa atatcgctaa agacgtattt tcttccctag taccatcacc
    13201 ttgttgattt tcaaacaaaa cagcggtcag acttacccca tcactcaaaa gttccaatcg
    13261 tcctacaggc gaatcatagt aactcttata ctccataaaa attccccctt tttctaatca
    13321 tacgccttaa ttgttaaatt agaaaatatt catgttcaca ttttttaaaa ataaataagt
    13381 cgtcaattac gtatataaac acgtaatacc agctatccct ttgctgcaat atacagttac
    13441 ataacttact acacgtgcta acctattact ttgtaaacca aatcttaaat taaaatattg
    13501 aaaatgcaat ggatccttaa tattttatta aacctataat tacttattaa aaataacaca
    13561 caatattcat aaagttttaa aaatattctg ttttatcacc tattattagt ggaaaagtac
    13621 aattgcaatt gtatatagtt tgcataacgc ttcaaaagta atttcttttt tgtttagttc
    13681 aaaaaaattt agaggtgatg ttatatgaat aacggttttt tcaatagcga ctttgattca
    13741 atttttcgaa gaatgatgca agatatgcaa ggttcaaatc aagtcggtaa caaaaagtac
    13801 tatattaatg gtaaagaagt ttcacctgaa gaactagcgc aactcacaca acaaggtagc
    13861 aatcaatctg ctgaacaaag tgcgcaagct tttcaacaag cagcacaaag acaacaaggg
    13921 caacaaggtg gcaacggcaa ttatttagaa caaattggtc gtaaccttac gcaagaagca
    13981 cgtgacggtt tattagatcc agtcattggt cgtgataaag aaattcaaga aactgctgaa
    14041 gtcttaagta gacgaactaa aaacaatcct atattagttg gagaagctgg tgttggtaaa
    14101 actgcgattg ttgaaggttt agcacaggca atcgttgaag gaaatgtacc agcagcaatc
    14161 aaagacaaag aaattatttc tgtagatatt tcatcattag aagctggaac gcaatatcgt
    14221 ggtgcttttg aagaaaatat tcaaaaatta atcgaaggtg ttaaatcttc acaaaatgcc
    14281 gtactattct ttgatgaaat ccatcaaatt atcggttcag gtgccacagg aagtgattca
    14341 ggtagcaaag ggttatctga tattttgaaa cctgcattaa gtcgtggtga gatttctatt
    14401 attggtgcaa caacacaaga tgaatatcga aacaatattc ttaaagatgc tgcattaacg
    14461 cgcagattta atgaagtgct tgttaatgaa ccaagcgcta aagatactgt tgaaatttta
    14521 aaaggtattc gcgaaaaatt cgaagaacac catcaagtaa aattaccaga tgacgtatta
    14581 aaagcatgtg ttgacttatc aattcaatat attccacaac gattattacc agataaagca
    14641 atcgatgtgt tagatattac agcagcacat ttatctgcgc aaagtccagc tgtcgataaa
    14701 gttgaaactg aaaaacgaat ttctgaatta gaaaatgata aacgtaaagc agtaagtgct
    14761 gaagaatata aaaaagctga cgacattcaa aatgaaatca aatcattaca agataaatta
    14821 gaaaacagta atggtgaaca tactgctgtt gctacagttc atgatatttc agatactatt
    14881 caacgattaa ctggcattcc agtttctcaa atggatgata acgatattga acgtttaaaa
    14941 aatatttcta atcgtttaag aagtaaaatc ataggtcaag atcaagctgt agaaatggtt
    15001 tcacgtgcaa ttcgccgtaa tcgcgctggg tttgatgacg gcaaccgtcc aattggtagt
    15061 ttcttatttg tcggccctac tggtgttggt aaaacagagc ttgctaaaca attagcaatt
    15121 gatctatttg gtaataaaga tgcacttatt cgacttgata tgagtgaata tagtgacaca
    15181 acagctgttt caaaaatgat tggtacaact gctggttatg tcggttatga tgacaattca
    15241 aatacgttaa ctgaaaaagt acgccgtaat ccatactcag tcattctatt tgatgaaatc
    15301 gaaaaagcaa atccacaaat tttaacattg ttattacaag taatggatga tggtaatttg
    15361 actgatggtc aaggtaatgt catcaacttt aaaaatacaa ttattatttg tacatcaaat
    15421 gctggctttg gcaatggcaa tgacgctgaa gaaaaagata ttatgcacga aatgaaaaaa
    15481 ttcttccgcc ctgaattcct taaccgcttc aacggcatcg ttgaattctt acatttagat
    15541 aaagatgcat tgcaagatat cgtcaactta ttattagacg atgtacaagt tacattagat
    15601 aaaaaaggta ttacgatgga cgtttctcaa gatgcgaaag attggttaat tgaagaaggc
    15661 tatgatgaag aattaggtgc acgtccatta agacgtattg ttgaacagca agtacgtgac
    15721 aaaattacag attattatct agatcataca gacgttaaac atgtggatat agatgttgag
    15781 gataacgaat tagtcgtaaa aggtaaataa cgacacttta acatatcgcg catcaaaaat
    15841 gagcatcagg tcgcccttgc ctgtgctcat ttttttaatt atttccctgg aaaatgattc
    15901 gctgtgtgct gttctgttcc acaacaatca cgattaatgt cacatgtacc acattttcct
    15961 tgtttcgaac gcttgaaaaa ttttactagt gtatataagg catatccgaa aattgctaaa
    16021 aaaattaaaa tgttaataat gactgacact ttaaccactc cttaaacaaa taaatgtccg
    16081 acttgataaa aaatgaatgt taagacatat gcagtgacta gaggataggc aactgcaagt
    16141 gccgtccatt tccatgaata agtctcttta cggattgctg ccactgtaga aacacaagga
    16201 atatacaata gtataaatat cataaatgca tacgcagata gcggtgtgaa ttgattttga
    16261 atcacattaa caaggcctgc atcacctgat gaatagataa tcgccatcga acttacgata
    16321 acttcttttg ctaaaaatcc tggcactaac gtagcacctg cttgccatgt tccaaatccg
    16381 agcggttgca ctaacatacc aaagaaacta ccaaccatat gtaaaaaact ttgattgata
    16441 ttcacattga taccatgtgg tcctacataa cttaatagcc aaatgactac tgagccgcca
    16501 aaaataaatg tacctgcttt acgaacaaag cccttagcct tttcccaagt actacgccac
    16561 aacgttttaa tggaaggcac acggtatgtt ggcaattcca caataaagat tgcattatca
    16621 ttttttaaaa tcgtcttagt aagtactgta ctgactaaaa atgccataat aatacctaaa
    16681 acatacaggc ttaatactac taaagattga ttctctttga aaaagatacc tacgaacaac
    16741 gcatacactg gcagtctagc agagcatgac atgaatggtg caattaatat cgttgttaaa
    16801 cgctcttttt cattttcaat actgcgcgca gccataatac tcggtacatt acaaccaaat
    16861 ccgataatca ttggtataaa agacttcccg cttaaaccga acgattccat aatacgatcc
    16921 attaaaaccg caatacgtgc catataacct gaatcttcta ataaagatat aaagaaaaag
    16981 agcacaacaa tttgtggtac aaagactaat actgatccta caccagcaat aatgccatct
    17041 gtaattaaat cttgtaaaaa tggtataacc cctagataat tcataatcgt cttcacacta
    17101 tctgtaaatg taccacctat aaatgcatcg agttgatccg acaaaggtgt gccaatccat
    17161 gtaaatgtag tttgaaagat caaccacata attgctagaa agataggcat ccctatatat
    17221 ttatgtgtta atatcttgtc tattctagag ctgaaatatt gcttatcttc atctggatac
    17281 gttaccacgt cttgcaataa cgtctcaata taatgattgc gtatacgctc catctctcga
    17341 cgaacagata cagcccctac ttgttcagca acttgatcac gtaaactcga caatttattt
    17401 acaacctctg aattaagttc gtttgcaatt tgcatgttat ttaataagaa ttgaatcgca
    17461 ataaacctag cttgatactt atcatgagat gtctctgtca ttattatttg acacatattt
    17521 ttaattgtct cttcaatctt ctcaccataa ttgattttaa aatgcggttg atacccttcc
    17581 cctagatgct ttatttcgcc aagtaaatat tttgttcctt tgcctgtacg tgccacaact
    17641 ggaaaaatag gtgtttttaa ctttttcatc aatttatgat aatcgatttt tatcccgcgc
    17701 tttgtagcta catcaatcat atttaatccg atgtatattg gttgattaag ttctaacaat
    17761 tgtactgtta attgcacatt tctttttagt tgactcgcat caacaatgtt aatgattcct
    17821 gaaaatgaat cgtttaataa atagtctgtc actacagttt catctttaga aatcggcgat
    17881 aaatcatatg tacctggtaa atcaattaat tgtcctacat tttctttaag tttccctact
    17941 tttttctcta ccgttacgcc actccagttg cctatatatt catacgaacc agttaaagcg
    18001 ttaaacaaag atgttttacc aacattagga tttcctaaaa tacaataatt ttccattcgt
    18061 ccggctccta ttcttctaat gcaatagaac aagcatcgca atgtctaata cttaactgtt
    18121 gtccgtttac ttcaataata catggccctt taaataaaca tttttgttta atcgttatga
    18181 tagcgtcatc tgttaaccca aaggcactta gacgatacaa catattctca ttagcaatat
    18241 ccattcgctt tattttataa gccttattca tttcaccatt tttaatgttt aacatactat
    18301 ttcgctctcc tattagaaat aataatcatt atcacttaaa aatcataacc cttaaaattg
    18361 tagctcgcaa tactttattt aaataatttt catttttcat gtaaaatttg cgacattgca
    18421 aaaatgtttt gtataatcac ttccatgcaa aaaacctccg cttcaaatgt cattgataga
    18481 catttaaagc ggaggcatgt acatgttatt ttatgattta tttcgtagat ttttttctaa
    18541 caattgattg agcttttcaa tatttttatt caactgttct atattcgtta cttgttgttt
    18601 tgaatttagt tgatgcttgt tttgataatc tattaaagca tcatataaga acaatgaact
    18661 tgcagattgc tgtttcactt cttggtacaa tgccatcgtt ttatcatcta gtactattga
    18721 atgtgcatca tcttgcccat gtacatcttg atgtttcgat acatttgttg caccttgtgg
    18781 atatacttta aaggttgtgt cgtatgtttc cttactatct ttggcttcag attcctgtga
    18841 ttcaaccgtt ttatattttt caagtgcatg tccttcaata tcaactcgtg gaataatgcg
    18901 attcaaccat gctggtaaat accacgaacc ttttccaaac aatttcgtta atgcaggaat
    18961 taacatcatt ctgactacga aggcatcaaa gagtacacca aacgctaatg ccatacccat
    19021 tgatttaatc atgacatctt cttggaatac aaacgcaaag aagacactaa acataattaa
    19081 tgcagcggct acaataacag gaccgctttc tttcaatcct actttgatag aataatcatt
    19141 atcccctgtt ttactatatt cttcatgaat tcgcgacata aggaagactt cataatccat
    19201 cgctaatcca aataagatac ctatagtaat aaccggtaaa aatgctagca ttggtcctgt
    19261 cgtttcaata ccaaacagac ctttcataaa accatcttgc attactaatg ttgtaaatcc
    19321 tagcgttgcc attaatgaca agacaaatcc taaaactgct tttaacggta ttagaattga
    19381 acggaagaca atcattaata agaaaaatgc caatacaaca attactgttg caaataaagg
    19441 tatcgcctca tttaactttt tagacatatc aatattaatg acactttgtc ccgaaatctc
    19501 cgttttgaac ccatatttat cttgtgcatc tttatgataa tctcgtaaat catgcactaa
    19561 atcatttgtg ctctctgcat taggcccttg cttaggtatc acgaccatca aagcataatc
    19621 attatcttta ctcatttgtg gcggcgtaac gatatctaca tttttcttat ctttaatatc
    19681 tttatataca gactgtaaat cttgttgtaa tccttgtgga tcatcttttt tatctttaac
    19741 atttatcaac atcggaattt ggccattaaa tccttcacca aatttatccg agattatatc
    19801 gtaagctttt ttctgtgtag aatctgctgg tttaacaccg tcatctggaa taccaagtcg
    19861 catatgacta actggtattg cagctgctac taatatgatt aaacctagta atactgccgc
    19921 aagtgcattt cctgtaataa atttagacca cggcgtatca atatcttttt taaaattaga
    19981 ctgcaatttg ttcactttaa tgcgtttatg gaaaatacta attaacgcag gtaataaagt
    20041 cagtgcactg aatacagcaa atataacact aattgccgaa gcaaacccca tcactgctaa
    20101 gaaatctatg ccaactagcg ataaaccaca tacggcaatc acaactgtca caccagcaaa
    20161 aacaactgca ctacctgctg ttcctattgc aagaccaatg cctttaatgt aatctgtttc
    20221 agttttcata acttgtcgat acctgaataa aataaacaaa gcataatcga taccaactgc
    20281 tagtccaatc attacggcta atgtcaatgt gacatttggt atatcaaatg cataagttaa
    20341 caaactgata atacctacac cagaggctag accaatcaat gcacttataa ttggtaatcc
    20401 tgcagcaatg actgaaccga atgtgattaa cagtacaaca aatgcaacaa taataccaac
    20461 tagttcagaa ttaccgccta cttctgtaga tgtcattcct gtaccagtta actctgtttg
    20521 tacattatga ttgtcacgta agtctttcaa atgactttta acattatctc tagagccatc
    20581 ttttaaagat gtttgactaa cgtcatatgt gatatctgca aatgcagttg ttttatcttt
    20641 actaatttgc ttattttcat atggatctga tattttatca atgtgcttgt catctttttt
    20701 tatatcctct aatgttttct taatgtcttc tgtaatgttc ggttgcacaa taccatcatc
    20761 tttagtcgtc ttaaagacaa cacgtatttg tgccttttca ctatcttgat taaaatgttt
    20821 ttcaatcttt ttattcgtat ctaacgactc taatcctgtc attttaatat cattgtcaaa
    20881 tttcggtgca tttgtagcaa gtggtatcaa tattgcagct acaatcacta tccatgcaat
    20941 gaccgcggac catttatgtt ttgcgatgaa tgtccccatc ttatataaaa attttgccaa
    21001 agtatattgc ctccttttaa aatcaacgtt atagtttaaa tatacagtgt agattattgt
    21061 tcgattatag tatctatccc cgacctctta aagaatcaat tggaaaattt tgtatattaa
    21121 actacacaca aaggagaaat gtagatgaaa gagactgatt tacgagttat aaagacaaaa
    21181 aaagcattgt cgagtagctt gctacaattg ttagaacagc aattattcca aacgattact
    21241 gtcaatcaaa tttgcgacaa tgcactcgta caccgcacaa cattttataa acatttttat
    21301 gataaatatg atcttctaga gtatttgttc aatcaattga ctaaagacta ctttgctaga
    21361 gatatcagtg accgtcttaa tcatccattc caaacgatga gtgatacgat taataataaa
    21421 gaggatttga gagaaatcgc agaattccaa gaagaagacg ctgaatttaa taaagtatta
    21481 aaaaatgtct gcattaaaat tatgcataac gatatcaaaa ataatagaga ccgtatcgat
    21541 attgacagcg acatcccaga taatctcata ttttatattt atgactcgtt gattgaaggt
    21601 tttatacatt ggataaaaga tgaaaaaatt gactggcctg gcgaagatat tgataacatt
    21661 ttccatagat taatcaatat taagattaaa tagtagatga gaaactcatg agcgttacca
    21721 acattcataa taaaaacgat agtgtacacg ttaatgaatt cgtgtactac tatcgttttt
    21781 tatttttatc gtgcttatcg ttattaaaat aactgataca caacacataa actatgaaga
    21841 aaaaaataaa tccgctatct aaatgacttt gactcagttg tttatatgac caaattgcta
    21901 atacaattcc cattattatt gaaataacgt atctcacatt cttataccta taatcctttt
    21961 ctaaaaatat ggttgctatt acttaatttt taaagttata aataaaaaga gccaaccgca
    22021 atggatggcc cttgttcatt atgaagcatt agaacatttc tgaaacaacc ttttgttcta
    22081 agaagtgtaa taagtagtct gggctacctg ttttagcgtc cgtacctgac attttgaaac
    22141 caccaaatgg atggtatcca acaactgctg aagtacaacc tctgttaagg tataaattac
    22201 ctacatcaaa ttcgtttacc gctttaatcc aatgctcgcg attatttgta atcactgcac
    22261 cagttaaacc gtaatctgta tcatttgcaa cctcaatggc ttcatcaaaa tcgttaactt
    22321 tcacaaagcc aacaactgga ccaaaaattt cttcttgcat gattctatct ttagatttaa
    22381 gtcctgaaat gattgttggt tctacaaagt aaccttttga atcatcagtg ccgccacctt
    22441 gttctaattt accttcttct ttaccaatct caatataatt tttaatctta tcaaattgtt
    22501 ttttattaat aactgggccc atatacgtat tgtctacagt attgcccaac gttaattctt
    22561 ttgttaattt gattgatttc tctaatactt cgtcataaac gtctttatgc acaattgcac
    22621 gtgaacatgc agaacatttt tgaccagaga aaccaaacgc tgacgttaca atagcttctg
    22681 ccgccatatc tgtatcaata ttttcatcaa ctacaatggc atctttacca cccatttcag
    22741 cgataacacg tttcaagaag ttttgacctt cttgaactac ggcactacgt tcataaattc
    22801 tagtacctgt cgcacgtgat cctgtaaatg taacgaaatg cgtatcttta tgatcaacta
    22861 agtaatcacc aatttctttc ggatcaccag gaacaaagtt aactacgcct tttggtaatc
    22921 ctgcttcttc taaaatttcc attaatttat aagcgatata aggtgtatcc tcagcaggtt
    22981 tcaataacac tgtattacct gcaacaactg gtgctaaagt cgtaccagcc ataatcgcaa
    23041 acgggaagtt ccacggtgga attgtaacac ctgtaccaat tgatttatag aaatatttat
    23101 tgtgttcacc ttcacgatca agtactggct taccttgagc caaatccatc attgaacgtg
    23161 catagtattc aataaaatca ataccttcag ctgcatcacc aactgcttca tcccatggct
    23221 tacctgcttc ataaaccata attgctgcaa tttccgcttt tcgacgacga ataattgccg
    23281 aaacacgtaa cataagctct gcacgatcat ttgctgacca tgttttccaa gatttataag
    23341 cttcgtttgc tgctttaaat gcatcttcaa catcttgttt tgttgccttt gatgcatttg
    23401 caatcacttg tgatgtgtct gcaggattga ttgatttaat tttgtcatct ttgaaaatct
    23461 tctctccatt aatcactaat ggtatgtctt gacctaattc tttttccacg tctttcaatg
    23521 ctttcttaaa catatccaca ttttcttgga ctgaaaaatc gtaaccaggt tcatttttaa
    23581 attctactac catgtacact taccccctat aaattttgaa agtggtttaa ccctttgatt
    23641 taatgatata acatcattta aactcatttt actatgatta aggttagttt tgcaatcgct
    23701 ttcattttta tgctttatca cttattctca agtattttga aattgattgg ttacttttta
    23761 aaatttatat gggtcgcaac tgctacttta tcgtttcgtc atttaatgtt tcggatggta
    23821 ggtcattatc aattttacga acgactttac aagggtttcc aaccgctaag ctgtgtggcg
    23881 gaatatcttt agtgacaaca ctaccagcac caatcacact gccttctcca atcgtcaccc
    23941 ctggtaacac ggctacatga ccgccaaacc aagtattact gccaatatga atgggtccgg
    24001 ctttttcata accttcattt ctatgataaa aattaagtgg atgtgttgct gtgtagaatc
    24061 cacaattagg tcctataaaa acattatcgc caattgtaat ctgtccacca tccataaaat
    24121 aacaattggt gtttacatag acattttttc ctagtttaac gttccaacca taatctgtat
    24181 caaaaggaat cgaaatactt acattgtctg ttgttgtttg aaataattga tcgattaatt
    24241 cctttctttt atttgtatca ctcggctttg tatgatttaa ttcaaagcaa atatctttcg
    24301 ctcgtgcacg ttcattgatt aagtcttgat caaagtttgc atcgtaccat ttttctgcta
    24361 acattttttc tttttcagtc attacacctt tcaactccta ataacttatt tacttgttta
    24421 aaagttaatc aaataaacat tcgcctatgc aactaatacg ctataacatt atgaaatcat
    24481 gaccttatca cccttatcta tacaattctc gcatcaaata ctgctaaagt agtagataaa
    24541 ttcaatacta cagacgcatt cattttttaa tctattaacg tacaatgtga gtaagagaaa
    24601 tataaaggag tatgatagcg atgagaatat taattacagg cacagttgct atcttaatca
    24661 ttctaggttt agttaaaacg atacaagtcg tcagttgtca gacaacaaag atgatgataa
    24721 agtcatccat cttaataatt tcaaaaattt acatgcgaaa gaatttaacc catctgattt
    24781 cttttaagtc acctaagaat tgcaaatcca gaagtcattt aagttttacc tttcattcat
    24841 acatccttta atattaatta cgacttcttt tatatagatg ctaaatagag agattgttgt
    24901 gcaatgtttg cacggcaatc tctctttttc tttttaaaat tggtaaaagt aaaacgcaac
    24961 gattgactta tatacctaga gggggtacat tagacgtgta acaatgaatc acagggaggc
    25021 aataatgtgg ctaatacgaa aaaaacaaca ttagatatca ctggtatgac ttgtgccgca
    25081 tgttcaaatc gtatcgaaaa gaaactgaat aaacttgatg acgttaatgc ccaagtgaat
    25141 ttaactacag agaaagcaac tgttgagtat aaccctgatc aacatgatgt ccaagaattt
    25201 attaatacga ttcaacattt aggttacggt gtcactgtag aaactgtcga attagacatt
    25261 acaggtatga cttgtgctgc atgctcaagc cgtattgaaa aagtgttaaa taaaatgaac
    25321 ggcgttcaaa atgcaacggt caatttaaca acagagcaag ctaaagttga ctattatcct
    25381 gaagaaacag atgctgataa acttgtcact cgcattcaaa aattaggtta tgacgcgtct
    25441 attaaagata acaataaaga tcaaacgtca cgcaaagctg aagcgctaca acataaattg
    25501 attaagctta tcatatcagc agtattatct ttaccactat taatgttaat gtttgtgcat
    25561 cttttcaata tgcatatacc agcactattt acgaatccat ggttccaatt tattttagct
    25621 acacctgtac aatttattat tggatggcaa ttttatgtag gtgcttataa aaacttaaga
    25681 aatggtggcg ccaatatgga tgtacttgtt gctgttggta caagtgcagc atatttttac
    25741 agtatttatg aaatggttcg ttggctaaat ggctcaacaa cgcaaccgca tttatacttt
    25801 gaaacaagcg ccgtactact taccttaatc ttattcggta agtatttaga agctagagcg
    25861 aagtctcaaa caaccaatgc gcttggcgaa ttattaagtt tacaagctaa agaagcacgc
    25921 attttaaaag atggtaatga agtgatgatt cctctaaatg aagtacatgt tggagataca
    25981 cttatcgtta aaccaggtga aaagatacct gttgatggca aaattattaa aggtatgact
    26041 gccatcgacg aatctatgtt aacaggtgaa tctatccctg ttgagaagaa tgttgatgat
    26101 actgtaattg gttcaacgat gaacaaaaac ggtactatta ctatgacagc aacaaaagtt
    26161 ggcggggaca ctgcgttggc aaatattatt aaagttgtcg aagaagctca aagttctaaa
    26221 gcgccgattc aacgattggc agatattatt tctggttatt tcgttcctat cgttgttggt
    26281 atcgcactat taatatttat cgtgtggatt actttagtta caccaggtac atttgaacct
    26341 gcacttgttg cgagtatttc cgttcttgtc attgcttgtc catgcgcatt aggacttgct
    26401 acaccaactt ctattatggt aggtactggt cgcgctgctg aaaatggcat tttatttaaa
    26461 ggtggcgagt ttgttgaacg cacacatcaa attgatacca tcgttttaga taagacgggt
    26521 accattacaa atggtcgtcc agtcgtgaca gattatcatg gtgacaatca aacgctacaa
    26581 ctgcttgcta ctgctgaaaa agattctgaa cacccattgg cagaagccat tgtcaattat
    26641 gcaaaagaaa agcaattaac attaactgag acaacaacat ttaaagcagt acctggccat
    26701 ggtattgaag caacgattga tcatcaccat atattggttg gtaaccgtaa attaatggct
    26761 gacaatgata ttagcttgcc taagcatatt tctgatgatt taacacatta tgaacgagat
    26821 ggtaaaactg ctatgctcat cgctgttaat tattcattaa ctggtatcat cgcagtggca
    26881 gatactgtca aagatcatgc caaagatgct ataaaacaat tgcatgatat gggcattgaa
    26941 gttgccatgt taactggcga taataaaaac actgctcaag ccattgcaaa acaagtaggc
    27001 atagatactg ttattgcaga tattttacca gaagaaaaag ctgcacaaat tgcgaaacta
    27061 cagcaacaag gtaagaaggt tgcgatggtt ggtgacggtg taaatgatgc acctgcatta
    27121 gttaaagctg atatcggtat cgccattggt acaggtacag aagttgccat tgaagcagct
    27181 gatattacta ttcttggtgg cgacttgatg cttattccta aagccattta tgcaagtaaa
    27241 gcaaccattc gtaatattcg tcaaaatcta ttttgggcat tcggctataa tattgccggt
    27301 atccctatag ctgcattggg cttacttgcg ccatgggttg ctggtgctgc aatggcacta
    27361 agttcagtaa gtgttgtcac aaacgcactt agattgaaaa agatgcgatt agaaccacgc
    27421 cgtaaagatg cctagattcc ttaataatga aggattcgtt ggtgattctg agataggcta
    27481 gtgattggct ctataatgtc gcggtttaca gttgggtctt cgctccaact gcatatatag
    27541 ttacactttt cgcttagcga attagtgtat cttacctaat agcttcgcct attaggttcc
    27601 atcattatta taaataataa gtacactatg gtttacagtt ggatcttcgc tccaactgca
    27661 taagagcctg agacataaat caatgttcta tgctctacaa agttataatg gcagtagttg
    27721 actgaacgaa aatgcgcttg taacaagctt ttttcaattc tagtcaactt tgccggggtg
    27781 ggacgacgaa ataaatttta cgaaaatatc atttctgtcc cactccctaa aaggaggtga
    27841 atgctatgtc acaagaaatt ttaaatgttg aaggtatgag ctgtggtcac tgcaaaagtg
    27901 ctgttgaatc tgcattaaat aatattgacg gtgtcacttc agctgacgtt aaccttgaaa
    27961 atggtcaagt aagtgttcaa tatgatgaca gtaaagttgc tgtatctcaa atgaaagacg
    28021 caattgaaga tcaaggttac gatgtcgttt aattaggcaa tattcaacgt catcaacacc
    28081 aaattaaaaa aatcgaactg atgagaatcc caacaatcca aattatctca tcagttcgat
    28141 ttttaattta ctcgtaacct agcatctcca gtctgcaata catctaatgc atcgacaatt
    28201 agatttttaa ctgcagcttc agtataaaac gcaatatgtg gtgttaatat gacatcttcc
    28261 ctgtcaatca acgattctaa caatggatcg ttcggtgttt tgcccctttg atcacttggg
    28321 aaaagtttgc gttcaaattc atacgtatca agtgctgcac ctttaatcac accattgtct
    28381 aatgcgtcta ataacgcctt agtatctact aaagaacctc tcgcacaatt gacaaatact
    28441 gcgccctttt taaaatgttt aaataattca gcattaaata gataatgatt atatttcgtt
    28501 gcaggtacat gtaatgtcac gatatcagca ccttcaaccg cttcctcaat cgtatctttg
    28561 taatcgacat acgttgcaat tttagcatta ggaaacgggt cgtatgcgac cacatcactt
    28621 tgataaccat tggcaaatat atcggctact acacggccaa ttcgacctgt accaataaca
    28681 gctactttta aatctttaat ggatttcgat aaaatagtag gttcccatct aaaatcatgc
    28741 tcccgcactt tcgtttgaat ttgattaaaa tgacgaacca cattaatagc ctggttcaca
    28801 gcaaactccg caattgaatt cggagagtat gacggcacat ttgacacaat caagttatac
    28861 ttgtttgcta actccaaatc atatgtatca aatccagcac tacgttgtgc gatttgttta
    28921 atacctagtt catttaatcg tttataaaca tgctctgata atggtatttg ttgtgatagc
    28981 gataagccat cataaccagc gacaccttca acattgtcat cagttaatgc ttctttagta
    29041 atatctacct caacatgatg tttctctgcc cacgccttga tataaggcat atcttcatca
    29101 cgtacactca tgattttaat ttttgtcatt ttaacatcac ccttaacttt attattcata
    29161 taaatatgct agttctgtta atcttattgc agcttcgtct aatttctggt catctaacgc
    29221 caatgaaatt ctcacataac gattaccatt ctctccaaat ggtttccctg gagcaacaag
    29281 tattgacttc tcttgcacta aaaattgctc aaattgctcg ctgtcataac caggcggtgt
    29341 ttccaaccat acatatatgc cacctttagc atgaacaaat ggcaaatcag cttttgcaag
    29401 catggcttcg aatcggtcac gacgtgtttt aaatacattg ctttgttctt ctaaaaaatc
    29461 atcataatga ttcaaagcat atattgcggc atcttgtaat gcaccaaaca tcccagcatt
    29521 tgtgtgcgtt tggtactttt tcaaagcttg aatcatatct ttattaccaa ctgcaaaacc
    29581 gactctaaaa cctgacatgt tataaccttt agacaaggaa taaatttcaa tcgcaacatc
    29641 tttcccattt tccgaagcaa gtatactagg atttttagcg tcgaaaccga aagcaccata
    29701 agcaaaatca tgcacgattt tagtgtctgt acctttaaat ttagctatcg cttcatcaaa
    29761 aacttctttc gtagctgtcg atccagttgg attatttgga tacgttaaat aaatgagttt
    29821 tgttttatct attatttgtg aatcaactgt ggaccaatct ggcaaataat gtggcggttc
    29881 taaattaagc ggaactggct tgccatcagc taaaagtaca cctgctaaat agtccgtgta
    29941 gcctggatca ggtagtaata catagtctcc tggattgata acacatgttg gtactgccac
    30001 taatccattt tttgtaccat ataaaatgca tacttcatct tctttatcta acgtcacatt
    30061 atattgtctt tgataaaaat ctacaatagc ttgcttgaac gcttctttac catgaaaagc
    30121 accatatttt tgattttcag gaatagttag tgctttttga aaatgatcaa taataccttg
    30181 tggcgtgggc ccatcaggga tcccaactgc catattaatt aatggcaatg gtccatgttc
    30241 gattttacgt cccatcgttt tcccgaaata actatcaggg atatttgcta atttgttaga
    30301 gatcatcaaa ttcctcctct atcattaaac atagcctggg cgactatcat aatcctaaca
    30361 acttgtatca ctctcattta gatggttaca atgacatcgc cattcaccgt tatgttcaac
    30421 agaacttatg acacacgttg tattgaatga atttattttc attttaggta ggtacattat
    30481 tattgtcaat attaggaatt ttcagattaa tatgcactca atcgttatga tttaactgtc
    30541 atgcatatcc gcatgcgcaa ccagttagat atgcttatat aaagtataac gcccctcaag
    30601 gtacgtattc aaacgtgaac cttaacaggc gcctattcat tgttaaataa aacttcttta
    30661 gaacatactt atttcactat gccttttact ttccccttat acttttctca catctttctc
    30721 ttagactact cccttatacg ccccgctcaa tatctttaat catttcgtct acagttattt
    30781 tcgcacttgt taagacaata ggaacgcctg cacctgggtg cgtacttgca cctgcaaaat
    30841 ataaatcttt ataatctcgc gatacatttt gtggacgata ataattactt tgcgctaaag
    30901 ttggcattaa accgaatgcc gaaccaaatt tcgcatgata cgtttgctca aaatcatttg
    30961 gcgtaaagat tgtttctgaa acaatatgcg attttatatc ttcaaatact tcaattgttg
    31021 ctaatttacg ataaataatt tcctttattt gttgcgtcaa agcttcatct gaccaatcga
    31081 ttccgctacc tgttttaagt tccggcgtcg gcattagcac ataaatacca gttttgcctt
    31141 ctggcgcaag tgatttatca gcgaccgctg gtacatacac ataaatagaa ggatcatatg
    31201 ataaacgtcc ctcaaatatt tcttcaatat tgcctctaaa gtcatctgaa aaaataacat
    31261 tatgaagtct cacttgatct gtcacatcaa tatctatacc gatatacatt aaaaatgctg
    31321 aacaagagta atctaagtct gcaattttat gtggtggata ctttttaata ggtgcaaaat
    31381 ctggcattaa tgattccgca acactaggga aatcagccgt acataaaatt ttatcaaatt
    31441 ttcttatgtc accattcact tttatcgcat cggcccgttt gaatttagga tcaataataa
    31501 tttgctcaat ttcagcattt agttcaatat taacgcctaa gtctttattt aattgcgcta
    31561 gcccttgagc cataccatac ataccgcctt taataaaatg cacaccaaac atcatttcaa
    31621 tcataggaat aattgaatat agtgacgggc ctcgttttgg atcaattcct atgtataacg
    31681 tttgaaacgc taaaagcttt tgtatctttt cgttatcaat ataatgttca attagctgat
    31741 ctgcatgatt taacgttttt aacttagcac cttgcacaag tgacgtcata ttataaaagt
    31801 cactcggttt gcgatacgtt ctttctaaga aatagcgacg tgcaatttca tattttttat
    31861 aaacatccgt taaaaaggac ataaaaccat gcgttgaacc aggttctata ctttctagca
    31921 tttgctgtaa ttcagctaaa tctgtaggca ccgttatacg atcatcgtgg tcaaaataca
    31981 catcgtaaat ataacgtaat tgtctcaatt caatataatc ttcataattt ttaccacacg
    32041 ctgtaaaaac atctttataa acatctggca tcatgacaat tgtgggaccc atatcaaatg
    32101 taaagccgtc tttctttaat tgattcatac gcccgcctac attattattt ttttcaaata
    32161 tcgtcacttc atgaccttga gaagcaatac gggctgccgc tgctaatcct gtgacacctg
    32221 caccaattac tgcaatcttc attattcaac cacctatatt ctatgatatt tactatttat
    32281 ttcatgaaac aactttgcct ttttccgctt atccacaaaa acacgttcat gtaatgtata
    32341 gttagcctgt ctcacttcgt ccagtatttc aatatatata cgtgctgcta attctatgat
    32401 tggttgtgct tcaatactaa atactttgat ttgatccata acatcttgaa aatctttttc
    32461 tgcgatagct gcataatatt cccataagtc aatataatga ttattaacac cattttggta
    32521 cacttcagca atatcaactt catattgctt taatcgttgc ttactaaaat atacccgttc
    32581 attgtcaaaa tcttcaccga catctcttaa tatattaatc aattgcaacg attcaccaag
    32641 tcttcttgcg acatcgtatg tctgatgtgt ttcatgatca cttaaaatcg gcgtcaatac
    32701 ttcacctact gtaccagcaa caccataaca atatccgaat aattcagcgt ccgtttcaaa
    32761 cattgtaaaa tgttgatctt tatatacagt atcaatgaga ttataaaaag attgaaaggc
    32821 gatattttta tgttgtgcaa catgctgaag cgccatcatg attctacgat cactttgaaa
    32881 gtgatgatgt tcatatgggt atttttcaat agattgtata tcttctttta tttgatttaa
    32941 aaattgaata tcgccataaa catctatact gtcatcaatt ttacgacaca cagcataaat
    33001 tgcccaaacc gcttttcttt gatcttctgg taacaagtca aaagcgtaag aaaagctttt
    33061 tgaatgtttc ttcatgattt tatgacaata tttaaaattc atatccatca ttgtcatact
    33121 agtcctccta tattgaaatg ccattacctt gatttattgt tctttagata tagaatattg
    33181 acgacctttc cattcaacta caccataacg atgcgtttgt ttccaagatt ggatgaaaat
    33241 tttagtaaaa aacataaata acaatggatg acataccatt aataaattag aaaattggcc
    33301 gacccttcga tgcagataaa taaattgtgt agtatagctc aagtaaagtg ctaccatttt
    33361 tcttacagac atttggcgat attttaaact aagacatagc cctaaaatac tcgctataga
    33421 accgaacaac cacaacacaa ttgctgtcat gatcttaggc tttgtgccac ctgccccagt
    33481 tgacaaatgc tttgtccatc cttcttgtaa tgactgaaat ccttcttgat acatgcgaaa
    33541 tgcaacaaat ggaaatcctt cataaactgt tacgggcaat gattgtgaag tatatgcact
    33601 tcctaaagca aatccttcaa taatatgatg gttcgcactt ttatgacctc cagttgcata
    33661 ataatcttct ttatttgtta atgtcaccgg tccaaatgca tgctggttag tccgaccgtc
    33721 gtctaaggta gaaaatacat tcataccaac gactgtcatt aaattaaata tcgctgaaaa
    33781 cccttcgtag aaacgctttg ttatatgata aggctgtacg cttaacaatc ctttttcacc
    33841 ttgtaattga tactgattaa tcaacgtttc aacagcatct ttccttaaga aagttacatc
    33901 agcatctaca aaggcaatac gattcgtaca tgcatgcgtc acaccttgat aacaagcatg
    33961 tgatttccca taccatttgc cgtcagtatc atcaacaaca tctactacag tcgcaccata
    34021 tgaacgtgct acacgtgctg tttcatctgt cgatccgtca ttcataacaa tgacatctac
    34081 tggaacttgc tgttgtatta tcgaatgtag taaatgacct attctttttt cttcatttct
    34141 agccggaata ataattgtta atgctttatg attgaagttc agcgttttcg tctttagctg
    34201 atgtctacga ttaaatatca atgcaccaca cgccatagac atggtcacta ttactgttaa
    34261 tattcgtgat aaccatttca tagatatcac ttcctattct tcgcttctcg cgcgtttatt
    34321 ttgtctgcga cttgttgccc acttaatgta accattggca tgccaccacc aggatttact
    34381 gatccaccta caaagtacaa gttttcaaaa tactgacttt ctttaggaaa tttaaatcct
    34441 ttgtttttct ttttatctgc tacaacacca tatattgcac cacgattaga acgataattt
    34501 ttttcaatat cctccggtgt ccaaacatct tcataaataa tgtgttttct taaatcagta
    34561 agtcccattt tttctaattt atccaaaatt ttatccctaa acttcgcata atcttcagtg
    34621 gtaaaaggct gatcttgaat atatggaata tgtggtaaga ctttgatatt ttcataacct
    34681 actggcgctt gtgtgtgatc agttttattc gtatttacta gataaatggt cggatcatct
    34741 ggcaatacct tttcatgaaa aacttgttga taattgagat aagcattttc cgtaaaaaag
    34801 aaattatgat gtgctaattg cgggtattgg caagcaacac ctaaatgcat cacatatcct
    34861 gagcttgccg gctcaaattc cctctctaat ttgtttaatc gttgagtatc aaggtgaatt
    34921 aaatatttat aagtaggtat gacttccata tttgaaataa tataatctgc ctttacaaac
    34981 tcacctgtat ctaatctgac acccgtcaca cgtctttgat atgttttaat attgtccaca
    35041 cgtgcacctg tatgaattgt gacaccttct tcacgcgcta gcttttccaa ggcattggct
    35101 aaatgatgga ttccaccttc tacataccaa aggccttgct cttgttgcat atggaataac
    35161 atagataata cagctggcgc atcgtatgat gaagaaccta catatttgat aaaatagcct
    35221 aacatttgtc gcaagtatgg attcgagata cgcttgtcta tggcctgttg catagtatgt
    35281 acataatcat aattaataag agcatttaat ggcccatgaa atttaataat ttgagatagt
    35341 gtatctaaac catagttaaa ataccctttt tcagtaatac gatcgattcg tcttgtataa
    35401 ttcaaataat tttgcagttc ctctatatcc tgtttcgaca atatcgcatt atgctgacct
    35461 gtttctttaa taccttcata caaatcgata gtcgtgccat ctggaaaaaa gctacgccat
    35521 tgatgtggca aacgcttgat tgtaacgtag tctgacattt gcttcttgct atattcgaat
    35581 aatttttcaa aaatataagg catcgttaaa atagatggac ctaaatcaaa gccaaagcca
    35641 tctgattcat gacgattcac tttgcctcct atatgagtat tttgttcata taatgagacc
    35701 gaatagccac tttgtgccat tcgaattgct gcagaaatcc cacctaagcc accaccaata
    35761 acgatgatat gtttagtcat gacgttcacc tcttcgtttt aatatgcgta gtaactgcgt
    35821 taatctcggt cgattatagc gttgcacaat aatgattgga acattagcta tcattgcata
    35881 gaaaatattt atatacttta ctaaacgaga gcctttattg aatatgatga ctggaagtat
    35941 cgatatccaa tgaatcaact ctgccctctt tgtctctata atcatttttt caatttcatc
    36001 agtattgatt gtcattaaat gacgctgatc atatatattt tgattaagct gatgaccatc
    36061 taaaatctta tgtttccatt ttcttacgta aaaccatttg ttccaaaatt ttccatgctt
    36121 ctcaaaatta aatgatttaa atattatgta cttctgacga aaatacttat caggaattct
    36181 tgtacctaaa tttgctatat ttagttgaac aattaaccaa tacatactac aaaaaaatgc
    36241 tgttttgata tattttttca tggttttcat ctaaattgaa tcactctcaa tcatactgac
    36301 atagtataaa ttatcacttt attacttagt tccctaaaga tcagtgaata aaccgctctc
    36361 atttatattt atataaaaca aaaacgccaa tactctcatg ttagagaata ttgacgtcat
    36421 ttgatttaca tttgattaat gaatgaaatt atatgaacct gcttggtttg ctgaaattgt
    36481 acgagacgta accacaccag caccatgtcc atagttcatt tctgaaacac gaacagaacc
    36541 gttgctgtta acgccttcaa cgtaagcaac atgaccgtaa tagccttgtg ttgtttgcat
    36601 gatagcacca acttttggtg tattgttcac tgtatagcca gatgatgcag ctgcgttagc
    36661 ccaattactt gcgttacccc atgttgaacc aattttccca ccaacacgat caaatacata
    36721 ataagtacat tgtcctgaag tatataagtt acttcctgat gcataaccat tagaaattga
    36781 acgaccattt gaagatggcg ctgcagttgt agtcacatga acattattac ttgttgtgct
    36841 ataacttgca cctgaaccac cagtataata gttatttgtt gctgtttggc tattgtttgt
    36901 atagttattg tattgatatg aatgattata gtagttattg taagatgcat tgttataact
    36961 ataagtattg tagttgttgt agtagtatgt gttgttttgc gttaattgac ttggattcca
    37021 atttcctgtc caagtgtaat gataattacc ttgtgcatca attgtatacg tatagctgta
    37081 tgattgagcg tcattagaat tatatccatt gttattttgt tctgcggctt gtgcatcatg
    37141 tcctgcaaat gcgatagtgg caagtcctgc tgtagcgatt gtagctgtaa cgattttttt
    37201 cataattaaa tatcctccca ataatcaaaa cactctatac atatagtcat tagcgactca
    37261 attattttaa attttatatt t
//
LOCUS       DAOBBH010000007        32939 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.007, whole genome shotgun sequence.
ACCESSION   DAOBBH010000007 DAOBBH010000000
VERSION     DAOBBH010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 32939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32939
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.007"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(52..678)
                     /locus_tag="P6L01_000204"
     CDS             complement(52..678)
                     /locus_tag="P6L01_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDP3029305.1"
                     /translation="MAKVLYITAHPFNELVSNSMAAGKAFIETYQQQHPDDEVKHIDL
                     FETYIPVIDKDVLTGWGKMSNGETLTDDEQMKVSRLSDILEEFLSADKYVFVTPMWNL
                     SFPPVVKAYIDAISIAGKTFKYSAEGPQGLLTDKKVLHIQSRGGYYTEGPAADFEMGD
                     RYLRTIMTFLGVPSYETIIIEGHNAEPHKTEEIKATSINNAEKLATTF"
     gene            887..1465
                     /locus_tag="P6L01_000205"
     CDS             887..1465
                     /locus_tag="P6L01_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDP3029306.1"
                     /translation="MTKRPKRILATIIIFLSLLFTIIYIDDIQKWFNQYTDKLTQNHK
                     GQGHSKWEDFFRGSRITETFGKYQHSPFDGKHYGIDFALPKGTPIKAPTNGKVTRIFN
                     NELGGKVLQIAEDNGEYHQWYLHLDKYNVKVGDRVKAGDIIAYSGNTGKQTTGAHLHF
                     QRMKGGVGNAYAEDPKPFIEQLPDGERSLYDL"
     gene            1849..2946
                     /gene="ugpC"
                     /locus_tag="P6L01_000206"
     CDS             1849..2946
                     /gene="ugpC"
                     /locus_tag="P6L01_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDP3029307.1"
                     /translation="MAELKLEHIKKTYDNNNTVVKDFNLHITDKEFIVFVGPSGCGKS
                     TTLRMVAGLESITSGDFYIDGERMNDVEPKNRDIAMVFQNYALYPHMTVFENMAFGLK
                     LRKVNKKEIEQKVNEAAEILGLTEYLGRKPKALSGGQRQRVALGRAIVRDAKVFLMDE
                     PLSNLDAKLRVQMRTEILKLHKRLNTTTIYVTHDQTEALTMASRIVVLKDGDIMQVGT
                     PREIYDAPNCIFVAQFIGSPAMNMLNATVEMDGLKVGTHHFKLHNKKFEKLKAAGYLD
                     KEIILGIRAEDIHEEPIFIQTSPETQFESEVVVSELLGSEIMVHSTFQGMELISKLDS
                     RTQVMTNDKITLAFDMNKCHFFDEKTGNRIV"
     gene            2959..4230
                     /locus_tag="P6L01_000207"
     CDS             2959..4230
                     /locus_tag="P6L01_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDP3029308.1"
                     /translation="MSKILKCITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPN
                     QLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIGQNDQLENISLDAPAGKGPDIFF
                     LAHDNTGSAYLQGLAAEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNK
                     KLVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSE
                     YDIHQLGLNSKHVVKNAERLQKWYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNIN
                     EYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQ
                     KYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNG
                     KNPKQALDEATNDITQNIKILHPSQNDKKGD"
     gene            4233..5501
                     /locus_tag="P6L01_000208"
     CDS             4233..5501
                     /locus_tag="P6L01_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDP3029309.1"
                     /translation="MTKRNPKLAALLSVIPGLGQFYNKRPIKGTIFFIFFISFISVFY
                     SFLNIGFWGLFTLGTVPKLDDSRVLLAQGIISILLVAFAIMLYVINILDAYRNAERFN
                     RNEEIKDPKARMVATWDKTFPYLLISPGTFLLIFVVVFPLIFMFGVAFTNYNLYNAPP
                     RHTLEWVGLDNFKTLFTIGVWRKTFFSVITWTLVWTLVATTLQIALGLFLAIIVNHPV
                     VKGKKFIRTVLILPWAVPSFVTILIFVALFNDEFGAINNDILQPLLGVAPAWLSDPFW
                     AKVALIGIQVWLGFPFVFALFTGVLQSISSDWYEAADMDGASSWQKFRNITFPHVIYA
                     TAPLLIMQYAGNFNNFNLIYLFNKGGPPVSGQNAGSTDILISWVYNLTFEFNNFNMGA
                     VVSLIIGFIVAIVAFIQFRRTSTFKDEGGL"
     gene            5503..6342
                     /locus_tag="P6L01_000209"
     CDS             5503..6342
                     /locus_tag="P6L01_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDP3029310.1"
                     /translation="MTKKKNILKAIGIYSFIAMMFVIILYPLLWTFGISLNPGTNLYG
                     AKMIPDNATFKNYAFLLFDDSSQYLTWYKNTLIVASANALFSVIFVTLTAYAFSRYRF
                     VGRKYGLITFLILQMFPVLMAMVAIYILLNTIGLLDSLFGLTLVYIGGSIPMNAFLVK
                     GYFDTIPKELDESAKIDGAGHMRIFLQIMLPLAKPILAVVALFNFMGPFMDFILPKIL
                     LRSPEKFTLAVGLFNFINDKYANNFTVFAAGAIMIAVPIAIVFLFLQRYLVSGLTTGA
                     TKG"
     gene            6416..7492
                     /locus_tag="P6L01_000210"
     CDS             6416..7492
                     /locus_tag="P6L01_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDP3029311.1"
                     /translation="MTIKVGIIGCGGIANGKHMPSLQKVENVEMIAFCDVDISKAASA
                     AEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAK
                     TTAEAQKMIDTAKSTGKKLTIGYQNRFRPDSQFLHQAAQRGDLGDIYFGKAHAIRRRA
                     VPTWGVFLNEEAQGGGPLIDIGTHALDLTLWMMDNYEPESVMGSTFHKLNKQHDAANA
                     WGSWNPDEFTVEDSAFGFIKMKNGATIILESAWAINSLEVDEAKCSLSGTKAGADMKD
                     GLRIHGEDMGTLYTKHVELENKGVDFYEGNEVDEAEEEAKAWIDAVVNDTEPVVKPEQ
                     AMVVTKILEAIYQSAKSGKAIYFE"
     gene            7517..8557
                     /locus_tag="P6L01_000211"
     CDS             7517..8557
                     /locus_tag="P6L01_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDP3029312.1"
                     /translation="MTKLKVGVIGVGGIAQDRHIPALLKLKDTVSLVAVQDINTVQMI
                     DVAKRFNIPQAVETPSELFKLVDAVVICTPNKFHADLSIEALNHGVHVLCEKPMAMTT
                     EECDRMIEAANKNHKLLTVAYHYRHTDVAMTAKKAIEAGVVGKPLVARVQAMRRRKVP
                     GWGVFTNKALQGGGSLIDYGCHLLDLSLWLLGKDMVPHEVLGKTYNQLSKQPNQINDW
                     GTFDHTKFDVDDHVTSYMTFANRASMQFECSWSANIKEDKVHVSLSGEDGGINLFPFE
                     IYEPRFGTIFESKANVEHNEDIAGERQARNFVNACLGIEEIVVKPEEARNVNALIEAI
                     YRSDLDNKSIQL"
     gene            8612..9580
                     /locus_tag="P6L01_000212"
     CDS             8612..9580
                     /locus_tag="P6L01_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDP3029313.1"
                     /translation="MKIGVFSVLFYDKNFEDMLDYVAESGLDMIEVGTGGNPGDKFCK
                     LDELLENEDKRQAFMKSITDRGLQISGFSCHNNPISPDPIEAKEADETLRKTIRLANL
                     LDVPVVNTFSGIAGSDDTAKKPNWPVTPWPTAYSEIYDYQWNEKLIPYWQDLAEFAKE
                     QDVKIAIELHAGFLVHTPYTMLKLREATNEYIGANLDPSHLWWQGIDPIAAIRILGQA
                     NAIHHFHAKDTYINQENVNMYGLTDMQPYGNVATRAWTFRTVGYGHSPYVWADIISQL
                     IINGYDYVLSIEHEDPIMSVEEGFQKACQTLKSVNIYDKPADMWWA"
     gene            complement(9941..10435)
                     /locus_tag="P6L01_000213"
     CDS             complement(9941..10435)
                     /locus_tag="P6L01_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HDP3029314.1"
                     /translation="MILSILLIFFCIRLVSLKISINHSKQLKADGAVEYGVKNSKFLA
                     ITHVLIYVLAGVEAFINKDTFSFANGIGLVILIFAYIMLFMVIKTLGGIWTLKLFILP
                     NHPIIKSGLYKITKHPNYFLNIIPELIGVLLLTHATYTTILLVPYAYFLYVRIKQEEK
                     LMNI"
     gene            10668..12047
                     /gene="uhpT"
                     /locus_tag="P6L01_000214"
     CDS             10668..12047
                     /gene="uhpT"
                     /locus_tag="P6L01_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDP3029315.1"
                     /translation="MNFFDIHKIPNKGIPLSVQRKLWLRNFMQAFFVVFFVYMAMYLI
                     RNNFKAAQPFLKEEIGLSTLELGYIGLAFSITYGLGKTLLGYFVDGRNTKRIISFLLI
                     LSAITVLIMGFVLSYFGSVMGLLIVLWGLNGVFQSVGGPASYSTISRWAPRTKRGRYL
                     GFWNTSHNIGGAIAGGVALWGANVFFHGNVIGMFIFPSVIALLIGIATLFIGKDDPEE
                     LGWNRAEEIWEEPVDKENIDSQGMTKWEIFKKYILGNPVIWILCVSNVFVYIVRIGID
                     NWAPLYVSEHLHFSKGDAVNTIFYFEIGALVASLLWGYVSDLLKGRRAIVAIGCMFMI
                     TFVVLFYTNATSVMMVNISLFALGALIFGPQLLIGVSLTGFVPKNAISVANGMTGSFA
                     YLFGDSMAKVGLAAIADPTRNGLNIFGYTLSGWTDVFIVFYVALFLGMILLGIVAFYE
                     EKKIRSLKI"
     gene            complement(12406..13164)
                     /locus_tag="P6L01_000215"
     CDS             complement(12406..13164)
                     /locus_tag="P6L01_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDP3029316.1"
                     /translation="MFKVVICDDERIIREGLKQIIPWGDYHFNTIYTAKDGVEALSLI
                     QQHQPELVITDIRMPRKNGVDLLNDIALLDCNVIILSSYDDFEYMKAGIQHHVLDYLL
                     KPVDHAQLEVILGRLVRTLLEQQSQNGRSLASCHDAFQPLLKVEYDDYYVNQIVDQIK
                     QSYQTKVTVSDLIQHIDVSESYAMRTFKDHVGITIVDYLNRYRILQSLQLLDRHYKHY
                     EIADKVGFSEYKMFSYHFKKYLQMSPSDYCKQAK"
     gene            complement(13157..14713)
                     /locus_tag="P6L01_000216"
     CDS             complement(13157..14713)
                     /locus_tag="P6L01_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDP3029317.1"
                     /translation="MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHR
                     TIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRELLKLIHQQPA
                     TLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFNNSTYMLKIYMANTPRLSEIKKD
                     SRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHEN
                     IEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPL
                     KNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTI
                     QYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIK
                     LHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGI
                     GMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYK
                     IPLSRGNVDV"
     gene            complement(14710..15678)
                     /locus_tag="P6L01_000217"
     CDS             complement(14710..15678)
                     /locus_tag="P6L01_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDP3029318.1"
                     /translation="MKSKIYILLLFLIFLSSCANTRHSESDKNVLTVYSPYQSNLIRP
                     ILNEFEKQEHVKIEIKHGSTQVLLSNLHNEDFSERGDVFMGGVLSETIDHPEDFVPYQ
                     DTSVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDIKIRGYQDLLQPILKGKIAYS
                     NPNTTTTGYQHMRAIYSMHHRVSDVHQFQNHAMQLSKTSKVIEDVAKGKYYAGLSYEQ
                     DARTWKNKGYPVSIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVA
                     EFDAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ"
     gene            16266..18515
                     /gene="pflB"
                     /locus_tag="P6L01_000218"
     CDS             16266..18515
                     /gene="pflB"
                     /locus_tag="P6L01_000218"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDP3029319.1"
                     /translation="MLETNKNHATAWQGFKNGRWNRHVDVREFIQLNYTLYEGNDSFL
                     AGPTEATSKLWEQVMQLSKEERERGGMWDMDTKVASTITSHDAGYLDKDLETIVGVQT
                     EKPFKRSMQPFGGIRMAKAACEAYGYELDEETEKIFTDYRKTHNQGVFDAYSREMLNC
                     RKAGVITGLPDAYGRGRIIGDYRRVALYGVDFLMEEKMHDFNTMSTEMSEDVIRLREE
                     LSEQYRALKELKELGQKYGFDLSRPAENFKEAVQWLYLAYLAAIKEQNGAAMSLGRTS
                     TFLDIYAERDLKAGVITESEVQEIIDHFIMKLRIVKFARTPDYNELFSGDPTWVTESI
                     GGVGIDGRPLVTKNSFRFLHSLDNLGPAPEPNLTVLWSVRLPDNFKTYCAKMSIKTSS
                     IQYENDDIMRESYGDDYGIACCVSAMTIGKQMQFFGARANLAKTLLYAINGGKDEKSG
                     AQVGPNFEGINSEVLEYDEVFKKFDQMMDWLAGVYINSLNVIHYMHDKYSYERIEMAL
                     HDTEIVRTMATGIAGLSVAADSLSAIKYAQVKPIRNEEGLVVDFEIEGDFPKYGNNDD
                     RVDDIAVDLVERFMTKLRSHKTYRDSEHTMSVLTITSNVVYGKKTGNTPDGRKAGEPF
                     APGANPMHGRDQKGALSSLSSVAKIPYDCCKDGISNTFSIVPKSLGKEPEDQNRNLTS
                     MLDGYAMQCGHHLNINVFNRETLIDAMEHPEEYPQLTIRVSGYAVNFIKLTREQQLDV
                     ISRTFHESM"
     gene            18538..19293
                     /gene="pflA"
                     /locus_tag="P6L01_000219"
     CDS             18538..19293
                     /gene="pflA"
                     /locus_tag="P6L01_000219"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDP3029320.1"
                     /translation="MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKI
                     SEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGEPLLQMPFLEKLFAELKENGVHT
                     CLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
                     LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLG
                     IAYELEDVEAPDDEAVKAAYRYVNFKGKIPVEL"
     gene            19615..21378
                     /locus_tag="P6L01_000220"
     CDS             19615..21378
                     /locus_tag="P6L01_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HDP3029321.1"
                     /translation="MKRISKDIWAVFKLLYQNKGRFSINALLLQLIMIFISSTYLILL
                     FNMMLKVAGQSQLTINNWMEIISHPASVILLIIFILSVAFLIYVEFSLLVYMVYAGFD
                     RQIITFKSIFKNAFVNVRKLIGVPVIFFVIYLMLMIPIANLGLSSVLTKNIYIPKFLT
                     EELMKTTKGIIIYGTFMIAVFILNFKLIFTLPLTILNRQSLFKNMRLSWQITKRNKFR
                     LVIEIVILELIIGAILTLIILGATYLAICVDEEGDKFLVSSILFVVLKSALFFYYLFT
                     KLSLISVLVLHLKQENVLDQPGLEFKYPKPKRKSRFFIISMVLAVTCFIGYNMYLLYN
                     NTINTNISIIGHRGFEDKGVENSIPSLKAAAKANVEYVELDTIMTKDKQFVVSHDNNL
                     KRLTGVNKNISESNFKDVVGLKMRQNGHEAKLVSLDEFIETAKQSNVKLLVELKPHGK
                     EPADYTQRVIDILKKHGVEHQYRVMSLDYDVMTKLKKEAPYLKCGYIIPLQFGHFKET
                     SLDFFVIEDFSYSPRLVNQAHLENKEVYTWTINGEEDLTKYLQTNVDGIITDDPALAD
                     QIKEEKKDETYFDRSIRILFE"
     gene            complement(21542..21886)
                     /locus_tag="P6L01_000221"
     CDS             complement(21542..21886)
                     /locus_tag="P6L01_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HDP3029322.1"
                     /translation="MTTQMKIKTYLVAGIKAALLDTTGIKLASKSETTSHTYQHQALV
                     DQLHELIANTDLNKLSYLNLDAFQKRDILAAHYIAKSAIRTKNLDQMTKAKQRLESIY
                     NSISNPLHSQNN"
     gene            22076..24052
                     /gene="coa"
                     /locus_tag="P6L01_000222"
     CDS             22076..24052
                     /gene="coa"
                     /locus_tag="P6L01_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylocoagulase"
                     /protein_id="HDP3029323.1"
                     /translation="MKKQIISLGALAVASSLFTWDNKADAIVTKDYSKESRVNEKSKK
                     GATVSDYYYWKIIDSLEAQFTGAIDLLEDYKYGDPIYKEAKDRLMTRVLGEDQYLLKK
                     KIDEYELYKKWYKSSNKNTNMLTFHKYNLYNLTMNEYNDIFNSLKDAVYQFNKEVKEI
                     EHKNVDLKQFDKDGEDKATKEVYDLVSEIDTLVVTYYADKDYGEHAKELRAKLDLILG
                     DTDNPHKITNERIKKEMIDDLNSIIDDFFMETKQNRPNSITKYDPTKHNFKEKSENKP
                     NFDKLVEETKKAVKEADESWKNKTVKKYEETVTKSPVVKEEKKVEEPQLPKVGNQQEV
                     KTTAGKAEETTQPVAQPLVKIPQETIYGETVKGPEYPTMENKTLQGEIVQGPDFLTME
                     QNRPSLSDNYTQPTTPNPILEGLEGSSSKLEIKPQGTESTLKGIQGESSDIEVKPQAT
                     ETTEASQYGPRPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETNAYNVT
                     TNQDGTVSYGARPTQNKPSETNAYNVTTHANGQVSYGARPTQNKPSKTNAYNVTTHAN
                     GQVSYGARPTQKKPSKTNAYNVTTHANGQVSYGARPTYKKPSETNAYNVTTHANGQVS
                     YGARPTQKKPSETNAYNVTTHADGTATYGPRVTK"
     gene            complement(24234..24341)
                     /locus_tag="P6L01_000223"
     CDS             complement(24234..24341)
                     /locus_tag="P6L01_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029324.1"
                     /translation="MCVRTRLVSSSSVRLSKAIIIAVIVVYHLDVRGLF"
     gene            complement(24639..25823)
                     /locus_tag="P6L01_000224"
     CDS             complement(24639..25823)
                     /locus_tag="P6L01_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDP3029325.1"
                     /translation="MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDG
                     KFNKNMIEDVIVGTAFPEGLQGQNIARTIALRAGLSDTVPGQTVNRYCSSGLQTIAIA
                     ANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQF
                     DVSREDQDAYAVRSHQRAYDAQRDGRFKDEIIPIRVNSVEYTNAGPKVHTNIFDQDEF
                     IRPDTTMEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFV
                     GYKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDI
                     SRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEY
                     VR"
     gene            complement(25853..28114)
                     /locus_tag="P6L01_000225"
     CDS             complement(25853..28114)
                     /locus_tag="P6L01_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HDP3029326.1"
                     /translation="MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLI
                     AKKSYDKITDKKRPLLFDLNLVSHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQ
                     QVLQHAKEDALFATNTSGIPINAIAQAFNEKDQERFFGLHFFNPPRIMKLVELIPTSH
                     TKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKLSIVDVD
                     ALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLFDNGAL
                     GRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDVIFNAQD
                     EAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVK
                     TRMEDELGDLPQWISDLDGGFYKQDETIEYATPVSHFVKDELWDKGDAKLSVTHDDQL
                     LLKLQSKNNVITDEFNDALVDAIDLLENDHYTSMVIYADGNNFSVGANLFLMKKAHED
                     GLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVAASETYI
                     GLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQASMTKVLTNIAFAKVSTNAFEARR
                     YGYLRDTDTIIFNTAQRVEVALKRAKYEAETNYIPNPRHQYIALGEDFKALIQGQLDA
                     QRRGHFISDHDYHIALNIATILAGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYER
                     IAHMLKTGKPLRN"
     gene            complement(28300..29511)
                     /locus_tag="P6L01_000226"
     CDS             complement(28300..29511)
                     /locus_tag="P6L01_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDP3029327.1"
                     /translation="MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESKYR
                     ENINQHWIDATVPEDYFKDLGELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDI
                     SLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLE
                     TVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKKTGKPHCFVVRPEQDGVDIEVID
                     NKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGAL
                     RATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVAT
                     STAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVI
                     GRALTGDSAFV"
     gene            complement(29623..31128)
                     /locus_tag="P6L01_000227"
     CDS             complement(29623..31128)
                     /locus_tag="P6L01_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HDP3029328.1"
                     /translation="MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYL
                     TSQGVKKGDVIGIFAPNDIAILDLLFACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKL
                     LFYAEKHLSSLTDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSG
                     TTGSPKGVMFSYESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGG
                     TLILQRYFNGEELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFRAMDYIIQGGSQ
                     PLPSIQAAFKQYGINIINGYGLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDN
                     GEEVPTGEIGELAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDDDGDIFIIDR
                     KKELIITGGENVLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYA
                     EILDQHMRSRLAGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL"
     gene            complement(31154..32731)
                     /locus_tag="P6L01_000228"
     CDS             complement(31154..32731)
                     /locus_tag="P6L01_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDP3029329.1"
                     /translation="MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLA
                     QHDTYHTPKDLTFILANDIHSLGAAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNN
                     DIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVT
                     INDETYLHYTFPSVDVALLRGTYADQQGNIYLTQEAYLSECYHVALNAKANHGKVIVQ
                     VKALVDDYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEP
                     ALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGG
                     FVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLN
                     GCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQY
                     SQLLEQEVYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDT
                     SIYKEKWGQLKQSIHKV"
BASE COUNT        10912 a         5483 c         6069 g        10475 t
ORIGIN      
        1 tacaataaaa aactgcatga cgttgaggag tcacacagtg taattaaaaa tttaaaaagt
       61 tgttgctaat ttttcagcat tattaatact agttgcttta atttcttcag ttttatgcgg
      121 ttcggcattg tgtccttcaa taatgattgt ttcatatgat ggcacaccta agaatgtcat
      181 aattgttctt aaataacggt cacccatttc aaaatcagca gcaggtcctt cagtataata
      241 tccaccacgt gattgaatgt gtaatacttt tttgtcagtt agtaaacctt gtggtccttc
      301 agcagaatat ttaaaagttt tacctgcaat tgaaatagca tcaatatatg ctttaactac
      361 aggtgggaaa gaaaggttcc acataggcgt tacaaataca tatttatctg cacttaaaaa
      421 ttcttctaaa atgtcactca atcttgaaac tttcatttgt tcatcatcag ttaacgtttc
      481 gccattactc atttttcccc aaccagttaa tacatctttg tcaataactg gaatataagt
      541 ttcaaataaa tcaatatgtt tcacttcatc atcaggatgt tgttgttgat atgtttcgat
      601 aaatgcttta ccagccgcca tagaatttga taccagttca ttaaaagggt gtgctgtaat
      661 atataatact tttgccattt gaaaattctc ctctgtttct gttattttct taagtataat
      721 tattatactc gatataaaat ttaatatcaa tcaaaatatt caaattacca tcattttctt
      781 catctatatt tggcagtact actaaagtat gagtgcattt aattatgaaa tagttgattt
      841 agaatatata cttaataccc aaaatatatg aaggatggat gccactatga caaagcgacc
      901 aaaacgtatt ttggcaacaa ttatcatttt tctttcacta ttatttacga ttatttatat
      961 agatgacatt caaaaatggt ttaaccaata taccgataaa ttgacacaaa atcataaagg
     1021 acaaggacac tcaaaatggg aagacttttt tagagggagt aggattactg agacttttgg
     1081 caaatatcaa cattcaccat ttgatggtaa gcattatggc attgattttg cattgccaaa
     1141 aggtacacca attaaagcgc cgacgaatgg taaagtaaca cgtatcttta ataatgaatt
     1201 aggcggcaag gtattacaga ttgccgaaga caatggagaa tatcaccagt ggtatctaca
     1261 cttagacaaa tataatgtca aagtaggtga tcgagttaaa gcaggtgata ttattgcata
     1321 ttcaggcaat acaggtaaac aaacgacagg cgcacattta cattttcaaa gaatgaaggg
     1381 tggcgtaggt aatgcatatg cagaagatcc taaaccgttt atcgagcagt tacctgatgg
     1441 ggaacgtagc ctatatgatt tgtagttata gaagagtgcc cgcagtctaa aaaattaagc
     1501 aatcattgtg tgagtatgat acttacataa tggttgcttt tttcaatgaa aatcgtaatg
     1561 ctaagtcata cttgtttgat ttagatatta cttaaaatgt aagacaaggt tgttagcatt
     1621 ggcagtgaaa tatcgcacat aaaaaaacat tattgtcaca ctagaaaata gttgtgtact
     1681 atatcaattt tctgtataaa agtttaattc tgacagtaat gtaaacgttt acaatttatg
     1741 attgacatta ataatgactg aatatatgat ttatgtaagt atttgtgcaa acgttttcac
     1801 aaagtgtatt gcacaatcaa actgtaaaca aagtatggga ggcataacat ggcagaacta
     1861 aagttagagc atattaaaaa gacgtatgat aacaacaata ctgtagtgaa agattttaat
     1921 ctacatatta ctgacaaaga attcattgta tttgttggac catcgggatg tggtaaatca
     1981 acaacattac gaatggttgc tggactagag tctatcacat ctggagattt ttatattgat
     2041 ggggaacgca tgaacgatgt tgaaccaaag aatagagata ttgcgatggt atttcaaaac
     2101 tatgcattat atccacatat gactgtattt gaaaatatgg catttgggct aaagctacgt
     2161 aaagtaaata aaaaagagat tgaacaaaaa gtcaatgaag cagctgaaat attaggatta
     2221 actgagtatc ttggtcgtaa accaaaagcg ttatctggtg gacagcgtca acgtgttgct
     2281 ttgggcagag ctattgttag ggatgcgaaa gtctttttaa tggatgaacc attatcgaat
     2341 cttgatgcga agcttcgagt acaaatgcgc acagaaatat tgaaattaca taagcgactt
     2401 aatactacga caatttatgt tacacatgat caaactgaag cattgacgat ggctagtcga
     2461 attgttgttt tgaaagatgg cgacattatg caagtcggca cacctagaga aatatatgat
     2521 gcccctaatt gcatatttgt ggcgcaattt atcggctcac cagcaatgaa tatgttgaat
     2581 gctacagttg aaatggacgg attgaaggta ggaacacatc attttaaatt acataataaa
     2641 aaatttgaaa agttaaaagc tgctggctac ttagacaagg aaattatttt aggtattcga
     2701 gctgaagaca ttcatgaaga accaatattt attcaaactt ctccagagac acaatttgaa
     2761 tctgaagtag ttgtatccga gttgttaggt tcagaaatca tggtacatag tacattccaa
     2821 ggaatggaat tgatttctaa attagattca agaacccaag tgatgacgaa cgacaagatt
     2881 acactagcat ttgatatgaa taagtgtcac ttttttgatg aaaaaacagg aaatcgtatc
     2941 gtctaagggg gagtattcat gtctaaaatt ttaaaatgta tcacgttagc cgtggtaatg
     3001 ttattaatcg taactgcatg tggccctaat cgttcgaaag aagatattga taaagcattg
     3061 aataaagata attctaaaga caagcctaac caacttacga tgtgggtgga tggcgacaag
     3121 caaatggcgt tttataaaaa aattacggat caatatacta aaaaaactgg catcaaagta
     3181 aagcttgtaa atattggtca aaatgatcaa ctagaaaata tttcgctaga cgctcctgca
     3241 ggaaaaggtc cagatatctt tttcttagca catgataata ctggaagtgc ctatctacaa
     3301 ggcttagctg ctgaaatcaa attatcaaaa gatgagttga aaggtttcaa taagcaagca
     3361 cttaaagcga tgaattatga caataagcaa ctagcattgc cagctatcgt agaaacaacc
     3421 gcacttttct ataataaaaa attagtgaaa aatgcaccgc aaacgttaga agaagttgaa
     3481 gctaatgctg ccaagctaac tgatagtaaa aagaaacaat acggtatgtt atttgatgct
     3541 aaaaatttct attttaatta tccgttttta ttcggcaatg atgattatat tttcaagaaa
     3601 aatggcagtg aatatgatat tcatcagcta ggactaaatt caaaacatgt cgtcaaaaat
     3661 gctgaacgat tacaaaaatg gtacgacaaa gggtatcttc ctaaggcagc aacacatgat
     3721 gtcatgattg gtctttttaa agaaggaaaa gtaggacaat ttgtcactgg accgtggaac
     3781 attaatgaat atcaagaaac gtttggtaaa gatttaggag taacaacatt acctacagat
     3841 ggtggcaaac ctatgaaacc atttctcggt gtacgtggtt ggtatttatc tgaatatagt
     3901 aaacataagt attgggctaa agatttaatg ctgtatatca ctagtaaaga tacattacaa
     3961 aaatatacag atgaaatgag cgaaattact ggacgtgttg acgtgaaatc atctaatcca
     4021 aatttaaaag tgtttgaaaa gcaagcacgt catgctgaac cgatgcctaa tattcctgaa
     4081 atgcgacaag tttgggaacc gatgggcaat gcaagcatat ttatctcaaa tggtaagaat
     4141 cctaaacaag cgttagatga ggcgacgaat gatataacgc aaaatattaa gattcttcat
     4201 ccatcacaaa atgataagaa aggagattag ttatgacgaa acgtaaccct aaattagcgg
     4261 cattattatc tgttatacct ggtttgggac agttttataa taaaagaccc attaaaggta
     4321 cgatattttt tatctttttc atcagtttta tttctgtatt ttatagcttt ttaaatattg
     4381 gtttttgggg attgttcaca ttagggacag tacctaagtt agacgattct cgtgtcttac
     4441 ttgcacaagg tattatttct atcttactcg ttgctttcgc aatcatgcta tatgtcatta
     4501 atattttaga tgcatatcgt aatgctgaac gatttaatcg caatgaggaa ataaaggatc
     4561 cgaaggcgcg tatggtggca acatgggaca agacgttccc atacttacta atctcaccag
     4621 gtacattctt attgatattt gtagttgtat ttccattaat atttatgttt ggagtagcat
     4681 ttacaaacta caatttatac aacgcgcctc cgagacacac attagaatgg gttggtttag
     4741 ataactttaa aacgttattc acaattggcg tttggcgcaa aacatttttc agtgttatta
     4801 cttggacatt agtatggacg cttgttgcaa cgacacttca aattgcatta gggctgtttt
     4861 tggcaattat tgtaaatcac cctgtcgtca aaggtaagaa atttatccgt actgtgttaa
     4921 tcctaccttg ggctgtacca tcatttgtga caattttaat atttgtagcg ttatttaatg
     4981 atgaatttgg tgcgataaat aatgatattt tgcaaccttt attaggtgta gcaccagcat
     5041 ggttaagtga tccgttttgg gcaaaagtgg cattaatcgg cattcaagta tggcttggat
     5101 tcccatttgt ctttgcactg ttcactggag tactgcaaag tatttcatca gattggtacg
     5161 aagcagcaga tatggatggt gcgtctagtt ggcaaaagtt tagaaacatc acattcccgc
     5221 atgtcattta cgccacagcg ccattgttaa ttatgcaata tgcaggtaat ttcaataatt
     5281 ttaatcttat ttatctattt aataaaggcg gtccaccagt gtcagggcag aatgctggta
     5341 gtacagatat cttgatatct tgggtgtata atctgacatt tgagtttaac aacttcaaca
     5401 tgggtgcagt tgtgtcatta attattggat ttattgttgc tattgtcgca tttattcaat
     5461 tcagacgtac aagtacgttt aaagatgagg gaggtttata agatgacaaa gaagaaaaac
     5521 atattaaaag caatcggtat ttacagtttt atagcgatga tgtttgtcat cattttatat
     5581 ccactactgt ggacatttgg catttccctt aatccaggta cgaacttgta tggtgccaaa
     5641 atgataccag acaatgcaac atttaaaaat tatgcattct tactattcga tgacagtagt
     5701 caatacctga cttggtataa aaatacgctt atcgtagcat ctgcaaatgc actgtttagt
     5761 gtgatatttg tcacgttaac agcatatgct ttttctagat atcgctttgt tggtcgtaaa
     5821 tacgggctga ttacattttt gattttacaa atgttccctg tattaatggc aatggtcgca
     5881 atctatattt tgctaaatac aattggttta ttagattctt tatttggact aacactggta
     5941 tatattggtg gatcaatacc gatgaatgcc tttttagtga aaggttactt cgatacgatt
     6001 ccaaaagaac ttgatgaatc tgccaaaatt gatggtgcag ggcatatgcg tattttctta
     6061 caaattatgc ttccattagc taagccgatt ttagcagttg ttgctttgtt caattttatg
     6121 ggaccattta tggactttat attacctaaa atactattaa gaagtcctga aaaattcaca
     6181 ttagcagttg gattgttcaa ctttattaat gataagtatg caaataattt cacagtgttt
     6241 gcagcagggg caattatgat tgcagtacct atagcaatcg tattcttgtt cttgcaacgc
     6301 tatttagtat caggtttaac aacaggtgcg acaaaaggtt agtttgaaat taggagtggg
     6361 gcagaattga taaagaacca ctaatgacga taaagattaa aaggaggacg ttatgatgac
     6421 gattaaagtt ggaatcattg ggtgtggtgg tattgcgaat ggcaagcaca tgccaagttt
     6481 acaaaaagtt gaaaatgttg aaatgatcgc attttgtgac gtagacattt cgaaagcagc
     6541 gagtgcggca gaagcatacg gaactgacaa tgcaaaggtt tatgatgatt acaaagcatt
     6601 gttaaaagat gacacgattg atgttatcca tgtttgtacg ccaaatgact cgcattgtga
     6661 aattactgta gcagggttgc atgccggtaa gcatgtgatg tgtgaaaaac caatggctaa
     6721 aacgacagca gaagctcaaa aaatgataga tacagctaaa tcaacaggta aaaaattaac
     6781 aataggttat caaaatcgct tcagaccaga tagtcaattt ttacatcaag cagcgcaacg
     6841 tggcgactta ggagacattt acttcggaaa ggcacatgcc attcgtcgtc gtgcagtacc
     6901 gacatggggt gtctttctaa acgaagaagc ccaaggtgga ggacctttaa tcgatattgg
     6961 tacgcacgca ttagatttaa cgttatggat gatggataat tatgaaccag aatcagtgat
     7021 gggttcaaca ttccataaat taaataagca gcatgatgcg gcaaacgctt ggggttcatg
     7081 gaatccagat gaatttacag ttgaagactc tgcgtttggc tttattaaaa tgaagaacgg
     7141 agcgacgatc attttagaat ccgcttgggc gattaattct ttagaagtgg atgaggcaaa
     7201 atgttcatta tcaggaacga aagcaggtgc tgatatgaaa gatggtctac gtattcatgg
     7261 tgaagatatg ggcacacttt ataccaaaca cgttgaattg gaaaacaaag gcgtcgactt
     7321 ttatgaaggt aatgaagtgg atgaagctga agaagaagca aaagcttgga ttgatgcagt
     7381 tgtaaatgat actgaaccag ttgtgaaacc ggaacaagca atggtagtta caaaaattct
     7441 tgaagcgatt tatcagtctg caaaatcagg caaagcaatt tactttgaat aacatcatac
     7501 ggtaaggagg cacatcatga caaaattaaa agttggtgtg ataggtgttg gtggcattgc
     7561 acaagaccgt catattccag cattgctgaa actcaaagac acagtctcat tagttgcagt
     7621 acaagatatt aatacagtgc agatgattga tgttgcgaag cgctttaata tacctcaggc
     7681 agttgagaca cctagcgagc tgtttaaact tgttgatgcg gtggtcattt gtacacccaa
     7741 taaattccat gctgatcttt ctatagaagc attgaaccat ggtgtccatg tcttatgcga
     7801 aaagccaatg gcaatgacga cggaagagtg tgatcgcatg attgaagcgg ctaataaaaa
     7861 tcacaaatta ttaactgtcg cttatcatta tcgtcacaca gatgtcgcta tgactgctaa
     7921 aaaagcaatt gaagcaggtg tggttggtaa accattagta gcacgtgtac aagcgatgcg
     7981 taggcgtaaa gtacctgggt ggggcgtttt taccaataaa gcgttgcaag gtggcggtag
     8041 tttaatcgat tatggttgcc acttgttaga cttatctttg tggctattag gtaaagatat
     8101 ggtgccgcat gaagtgctag gaaaaacata taatcaattg agcaaacaac cgaatcaaat
     8161 taatgattgg ggaacatttg atcacactaa atttgatgtc gatgatcatg ttactagtta
     8221 tatgacattt gccaatcgag caagcatgca gtttgaatgt tcgtggtctg caaatataaa
     8281 ggaagataag gtacacgtta gtttatcagg agaagatggc ggtatcaatt tatttccatt
     8341 tgaaatatat gagccccgct ttggaactat ttttgaaagc aaagctaatg ttgagcataa
     8401 cgaagacatt gctggtgaga gacaggcgcg taactttgtc aatgcgtgtt taggtataga
     8461 agagattgtg gtgaaaccgg aagaagcacg caatgtaaat gcccttatag aagcgattta
     8521 tcgtagcgat cttgataaca agagcataca actttaatga ttattatata taatacaaaa
     8581 ttctcaatat aaaaagaagg agtgcttttc aatgaaaata ggtgtatttt cagtattatt
     8641 ttacgataaa aattttgaag atatgttaga ttatgtcgca gaatctggat tggatatgat
     8701 tgaagttgga acaggtggta acccaggaga taaattttgt aagttagatg agttgttaga
     8761 aaatgaagac aagcgacaag catttatgaa gtcaatcaca gacagaggct tacaaataag
     8821 tggtttcagt tgtcataaca atccaatttc tccagatccg atagaagcga aagaagccga
     8881 tgaaacgtta cgtaaaacaa tccgtttagc aaatctatta gacgtgccag ttgttaatac
     8941 attttctggc attgcagggt cagatgatac cgctaaaaag cctaattggc ctgttacacc
     9001 ttggccaaca gcctactctg aaatttatga ttatcagtgg aatgaaaagt tgataccata
     9061 ttggcaagat ttagctgagt ttgcaaaaga gcaagatgta aaaattgcca tagagttaca
     9121 tgcaggattt ttagttcata caccatatac gatgttaaag ttacgtgagg ctacaaatga
     9181 atatatcggt gctaacttag atcctagtca tttatggtgg caaggtattg atccaattgc
     9241 tgcgattcgc atattaggcc aagcaaatgc aattcatcac ttccatgcta aagatacgta
     9301 tattaatcaa gaaaatgtaa atatgtatgg tctaactgat atgcaaccat atggtaacgt
     9361 tgcgacaaga gcatggacat tccgtacagt tggttatgga catagtccat atgtatgggc
     9421 agatatcata agtcaactta ttattaatgg atatgattat gtattaagta ttgaacatga
     9481 agatcctatt atgtcagtag aagaaggttt ccaaaaagct tgtcaaactt tgaaatctgt
     9541 taatatttac gacaagccag cagacatgtg gtgggcataa tacgaactcg aggttagtct
     9601 gaagtttgtc tgaagtaaga ctggtggcag tgttgaatga atgcatatgt cgccaagcca
     9661 ttgccaaaaa tttcacatct taaatcatgt cattgtttgt aaagaaggtg tactttatat
     9721 aagtgtttag cgatggtcat acccattcac agtaacaatc ctcaccattg aaaagagtat
     9781 ataacctttt cgatagtgag gtatatgata ataaaaaaag cctgttgtca caatggtcat
     9841 agacacgaca tactttaaag gcttctgaat ataatatttc agaacgcact ttaaagatgg
     9901 acgtcgatgt cgactaatgt gatgacaggc tttcatcttt ttaaatattc attaatttct
     9961 cttcttgttt aatacgtaca tataagaaat acgcatacgg tactaataaa atagttgtat
    10021 atgttgcgtg tgttaataat aatacaccga ttaattcagg aatgatgttt aagaagtagt
    10081 ttgggtgttt tgtaatttta tataatccag atttaataat aggatggtta ggtaaaatga
    10141 ataattttaa tgtccaaata ccacctaaag ttttaataac cataaataac atgatataag
    10201 caaagattaa tataactaag ccaataccat ttgcaaagct aaatgtatct ttattaataa
    10261 atgcctctac accagccaat acataaatta aaacgtgtgt tattgctaaa aacttcgaat
    10321 ttttaacgcc atattcaact gcaccgtctg cttttaattg ttttgagtga ttaatagata
    10381 tctttaagct gacaagtctg atacagaaaa agataagtaa tatagataga atcatgatgt
    10441 cctccgtcat tatgtcatat gtataagcgt tgattttgac aacataaagt attttataga
    10501 taaaacttgt caaatactat taactattta ttaattttag tacataaata tgtttctaag
    10561 tatgtgttta tgttcagtat tttggataat ttaataattt taaggatatt aagcgcttac
    10621 accgacgtga tatatttggc ttaacgaaaa tgattgaggt gacagagatg aacttttttg
    10681 atatccataa gattccgaac aaaggcattc cattatcggt acaacgtaaa ttatggctta
    10741 gaaacttcat gcaagctttc tttgtagtgt tctttgttta tatggctatg tatttaattc
    10801 gaaacaactt taaggcggcc caaccgtttt taaaagagga aattggatta tctacattag
    10861 aacttggtta tatcggatta gcatttagta tcacgtacgg tttagggaaa acattacttg
    10921 gatattttgt cgatggacgt aacacaaaac gtattatctc gttcttactt atcttatctg
    10981 cgattacagt tttaattatg ggatttgttt taagttactt tggttctgta atgggattat
    11041 taattgtact ttggggactt aacggggtgt tccaatcagt tggtggacct gcaagttatt
    11101 caacgatttc aagatgggcg ccaagaacga aacgtggccg atacttagga ttttggaata
    11161 catcacataa tatcggtggt gccattgcag gtggtgttgc actttggggt gctaatgtat
    11221 tcttccatgg aaatgttata gggatgttca ttttcccatc ggtgattgca ttacttattg
    11281 gtatcgcaac attatttatc ggaaaagatg atccagaaga attaggatgg aatcgtgctg
    11341 aagaaatttg ggaagagccg gtcgataaag aaaatattga ttctcaaggt atgacgaaat
    11401 gggagatctt taaaaaatat atcctgggaa atcctgttat atggattcta tgtgtttcaa
    11461 acgtctttgt atacattgta cgaatcggta ttgataactg ggcaccgtta tatgtgtcag
    11521 agcatttaca ctttagtaaa ggcgatgcag ttaatacgat attctacttt gaaattggtg
    11581 cattagttgc aagtttatta tggggctacg tatcagactt attaaaaggt cgtcgtgcaa
    11641 ttgtagctat tggctgtatg tttatgatta catttgttgt cttattctac acaaatgcta
    11701 caagtgtcat gatggttaac atttcattgt ttgcattagg tgcgttaatc tttggtccgc
    11761 aattattaat tggtgtatca ttgactggtt ttgttcctaa aaatgccatc agtgtagcaa
    11821 acggaatgac aggttcattc gcgtatctat tcggtgactc aatggcgaaa gttggtttgg
    11881 cggctattgc tgatccaaca cgtaacggtt taaacatctt tggatataca ttaagtggat
    11941 ggacagatgt tttcatcgtc ttctatgttg cattattcct aggcatgatt ctattaggaa
    12001 tcgttgcttt ctatgaagaa aagaaaatta gaagtttaaa aatttaatat aaatcggatt
    12061 aaaagtatcg ccaatctatt gcaatatagt tggcaatcct gccccgacgg catgtgcgtg
    12121 aagagatgaa agatactgct tctacccttg caaatatatc atctctatgt ctcggggcag
    12181 atcataattc cctgttatga agtatcctta tttgcccgac ttagggtgac tcaatgaatt
    12241 tactccttac aataaagaca tatagcggtg tcaatattgt agggagtatt gttttatatt
    12301 taaactctct aaaaagcgga ctgaaagaaa agtgaaaact tctctatcag tccgcttttt
    12361 catagaacaa aatgtaggcg ccataatcat tagttatgtg ctaatctatt tagcttgctt
    12421 acaataatca cttggcgaca tttgtaaata ttttttaaaa tgatagctaa acattttata
    12481 ctctgaaaag cctactttgt ctgcaatttc atagtgtttg taatgtcgat ctaacaattg
    12541 cagagattgt aaaatacgat agcgatttaa ataatcgaca attgtaatac caacatgatc
    12601 tttaaatgtt cgcatcgcat acgattcact aacatcgata tgttgaatta aatctgaaac
    12661 agtcactttc gtttgataag attgcttaat ttgatcaaca atctgattta cataataatc
    12721 atcgtattct acttttaata gtggttggaa ggcatcatga caagatgcta agctacggcc
    12781 gttctgtgat tgttgctcta ataaggtacg gacaagtctt cctaaaataa cttctaattg
    12841 tgcatggtct actggtttta ataaataatc aagaacatga tgttgaatgc cggctttcat
    12901 atattcaaag tcatcgtaac tcgataatat gatgacatta caatctagaa gcgcaatatc
    12961 attgagtaaa tcgacgccat ttttacgtgg catacgaata tcagtaatta ctaattctgg
    13021 ctgatgttgt tgaattagtg ataatgcttc aacaccatct ttagcagtgt atattgtatt
    13081 gaaatgatag tctccccaag gaatgatttg ctttaaacct tctcgaataa ttcgttcatc
    13141 atcacaaata actaccttaa acatctacat tcccccttga aagtggtatt ttataacaaa
    13201 ttaacgtacc ttgattacgc tttgaaaaaa tatgaagtcg tgcatgtgaa ccatattgaa
    13261 tcattgcttt attgtgtaaa tgatttaatc ccaaatgctt agtatcaaat acatcattat
    13321 taagagattg gcgtacatat tgcaggcgag atgacgacat cccgatacca ttgtcgcaaa
    13381 ctaaaacatg taaattctga cgtgccaatg tcaggcgtat agtaatgtcc aatgactcag
    13441 tatctctacc atgtttaata gcattttcta tgagtggctg aagcatcatt ttaccaattg
    13501 tctggtgacg cgcttcttca gaactttcaa tatggagctt aatcatgtca tcaaaacgga
    13561 tgttttgtat tgcaacatac tgttcaatgt agttcaactc ttcgtttaat tccactgtat
    13621 gtgagtttgt acgtaatgag taacgtaaca tttgcgataa ttgttggacc acagtttgtg
    13681 ctaatttcgg agataacgta attaaatatt gtattgtttg catcgtattg aataggaaat
    13741 gaggctggaa ttggcgttct atttccttta actgaatatc acgcaagcga cgttctgtat
    13801 gctcgataga atggatcagt tgctcatttg attcaaataa atcgtaaata taattattaa
    13861 tttcttctag ttcactgttg ttttttaaag gcgtatatgt acctagatga cgatttttgg
    13921 catagtaaat tttttgaata atcgtttcga tatcttttgt ttgtcgttta gccatattat
    13981 ctgcgctaat gaaaccaaat attactagta aaacaagaac tacggccata acaattaaca
    14041 acgtgatacc atcttcaatg ttttcatgta tatctttata aataatgaga cgatggtcag
    14101 catggtttaa ttttacagat tcattcataa atccgaattg ttgcggtcta tacttttcac
    14161 ctatagtaaa acggtcatcg ttggcgtata aaatattgtc atattgatca acgataagtg
    14221 cgaattgtcg gctatctttc ttaatttcac ttaaacgtgg ggtgttagcc atataaattt
    14281 taagcatata tgtactatta ttgaatttaa gctgatgcgt tgaaaataaa tacatatttt
    14341 tagtgtttaa atgttcataa ttattggtta taaactgatt tggtccagat aattcataat
    14401 aaagtgttgc gggctgttgg tgtattaatt ttaataattc acgttttgta gcggtaacat
    14461 catgatgatt tgttaaatcg agctcttgaa acgaattatt atgctgtgta ataaatgtct
    14521 gaatctgctt ttcagtgtga tgtaaagatg actgactttc atcaacatgt tgatgaatcg
    14581 tacgatgctc aatccaaata tagatggcat agaagcttac tagtccaata ataatgacta
    14641 aaaatactgg aaaaatagta gacgcaaata acgatcgtct caattgatgt ctataaggtt
    14701 tgtatgccgt cattgaatca tctccaaaaa tttatgatgt ggaatgtccg gtaatttaga
    14761 tttcggtatt aaaggtatgt tcttaagatt ttcgatagac tgatcgcttt gttcactaac
    14821 atcctttcga attgacttgg catcgaactc tgcaactaat cgttgttgta ctgagcggct
    14881 tgttaaatat tgcactaact ttttacgctt aggatgaggg tgtgcatttt taactaaagc
    14941 aataccatca acatttaaca ttgttccttc aattggataa acgattgata caggataacc
    15001 tttgtttttc catgtgcgtg catcttgttc gtagcttaga cctgcgtaat atttaccttt
    15061 tgcaacatct tcaatgactt tagacgtctt tgacagttgc atcgcatggt tttggaattg
    15121 atgcacatca cttactcgat gatgcatgct ataaatagcg cgcatatgtt gatagcctgt
    15181 cgttgttgta tttggatttg agtacgcaat tttaccttta agtataggtt gtaataaatc
    15241 ttgataacct cgaatcttaa tatctccttg taaatctgaa ttcactacta taactgttgg
    15301 cattaataga aaactagtaa catatttatt gttcgagcga taatcctcta attgctgtgt
    15361 tacagatgta tcttgatagg gaacaaaatc ttctggatga tcaattgttt ctgataacac
    15421 accacccata aagacgtcac cacgctccga aaaatcttcg ttatgcaagt ttgaaagcag
    15481 tacttgagta gatccatgtt tgatttcaat tttgacatgc tcttgttttt caaattcatt
    15541 taaaattgga cgaatcaagt ttgattgata cggagaataa actgttaata catttttatc
    15601 tgattcagag tgacgcgtat tagcgcatga tgataaaaaa atgagaaata atagcaagat
    15661 ataaattttt gatttcatga tatcccatca attctatgta tattttaata caataatttt
    15721 agcaataaat gacgcataag taatgttaaa tatttagaaa tgtttataga tgacttgtta
    15781 agacgttgca aatgttgtga tagcacaaaa tttttgtttg tcaagacgat ttaccgaggc
    15841 tgtaaaatca aactgttata ttttatttgt agctgttata taaaaatcgg caagatattg
    15901 aacggttcaa aagtgaattt ttacgtcaat aaaagtattt aatccagtct cttcatatat
    15961 aaaagtaaat ctttctaagt gttgatttaa cgcttatcaa caatcatttt ttataaacaa
    16021 atatatactc ctaaattaac ttttaaagca atgaaaatag tgaacattat aactgttgtg
    16081 taacagaatg caattagcat attactgtta cacaaattag tacagtttct atgttttgac
    16141 atacatttga tgaaaattgt acataattta tgtgaaaaaa atcacaacaa acatgctaca
    16201 atgactatga aaacgttaac atagcatttc aaattcacaa cattatacag atggaggcgt
    16261 ttagtatgtt agaaacaaat aaaaatcatg caacagcttg gcaaggattt aaaaatggaa
    16321 gatggaacag acacgtagat gtaagagagt ttatccaatt aaactacact ctttatgaag
    16381 gtaatgattc atttttagca ggaccaacag aagcaacttc taaactttgg gaacaagtaa
    16441 tgcagttatc gaaagaagaa cgtgaacgtg gcggcatgtg ggatatggac acgaaagtag
    16501 cttcaacaat cacatctcat gatgctggtt atttagacaa agatttagaa acaattgtag
    16561 gtgtacaaac tgaaaagcca ttcaaacgtt caatgcaacc attcggtggt attcgtatgg
    16621 cgaaagcagc ttgtgaagct tacggttacg aattagacga agaaactgaa aaaatcttta
    16681 cagattatcg taaaacacat aaccaaggtg tattcgatgc atattctaga gaaatgttga
    16741 actgccgtaa agcaggtgta atcactggtt tacctgatgc atacggacgt ggacgtatta
    16801 tcggtgacta tcgtcgtgta gctttatatg gtgtagattt cttaatggaa gaaaaaatgc
    16861 acgacttcaa cacgatgtct acagaaatgt cagaagatgt aattcgttta cgtgaagaat
    16921 tatcagaaca atatcgtgca ttaaaagaat taaaagaact tggacaaaaa tatggtttcg
    16981 atttaagccg tccagcagaa aacttcaaag aagcagttca atggttatac ttagcatacc
    17041 ttgctgcaat taaagaacaa aacggtgcag caatgagttt aggtcgtaca tcaacattct
    17101 tagatatcta tgctgaacgt gaccttaaag caggcgttat tactgaaagc gaagttcaag
    17161 aaattattga ccacttcatc atgaaattac gtattgttaa atttgctcgt acacctgatt
    17221 acaatgaatt attctctgga gacccaactt gggtaactga atctatcggt ggtgtaggta
    17281 ttgacggacg tccacttgtt acgaaaaact cattccgttt cttacactca ttagataact
    17341 taggtccagc tccagaacca aacttaacag tattatggtc agtacgttta cctgacaact
    17401 tcaaaacata ctgtgcaaaa atgagtatta aaacaagttc tatccaatat gaaaatgatg
    17461 acattatgcg cgaaagctat ggcgatgact atggtatcgc atgttgtgta tcagcgatga
    17521 caattggtaa acaaatgcaa ttcttcggtg cacgtgcgaa cttagctaaa acattacttt
    17581 acgcaatcaa tggtggtaaa gatgaaaaat ctggtgcaca agttggtcca aacttcgaag
    17641 gtattaacag cgaagtatta gaatatgacg aagtattcaa gaaatttgat caaatgatgg
    17701 attggctagc aggtgtttac attaactcat taaatgttat tcactacatg cacgataaat
    17761 acagctatga acgtattgaa atggcattac atgatacaga aattgtacgt acaatggcaa
    17821 caggtatcgc tggtttatca gtagcagctg actcattatc tgcaattaaa tatgcacaag
    17881 ttaaaccaat tcgtaacgaa gaaggtcttg tagtagactt tgaaatcgaa ggcgacttcc
    17941 ctaaatacgg taacaatgac gaccgtgtag atgatatcgc agttgattta gtagaacgct
    18001 tcatgactaa attacgtagt cataaaacat atcgtgattc agaacataca atgagtgtat
    18061 taacaattac ttcaaacgtt gtatacggta agaaaactgg taacacacca gacggacgta
    18121 aagctggcga accatttgct ccaggtgcaa acccaatgca tggccgtgac caaaaaggtg
    18181 cattatcttc attaagttct gtagctaaga tcccttacga ttgctgtaaa gatggtattt
    18241 caaatacatt cagtatcgta ccaaaatcat taggtaaaga accagaagat caaaaccgta
    18301 acttaactag tatgttagat ggttacgcaa tgcaatgtgg tcaccactta aatattaacg
    18361 tatttaaccg tgaaacatta atagatgcaa tggaacatcc agaagaatat ccacagttaa
    18421 caatccgtgt atctggttac gctgttaact tcattaaatt aacacgtgaa caacaattag
    18481 atgtaatttc tcgtacattc catgaaagta tgtaacaaaa tttaaggtgg gagcactatg
    18541 cttaagggac acttacattc tgtcgaaagt ttaggtactg tcgatggacc gggattaaga
    18601 tatatattat ttacacaagg atgcttactt agatgcttgt attgccacaa tccagatact
    18661 tggaaaatta gtgagccatc aagagaagtc acagttgatg aaatggtgaa tgaaatatta
    18721 ccatacaaac catactttga tgcatcgggt ggcggtgtaa cagtcagtgg tggcgaacca
    18781 ttgttacaaa tgccattctt agaaaaatta tttgcggaat taaaagaaaa tggtgtgcac
    18841 acttgcttag acacatcggc tggatgtgct aatgatacaa aagcatttca aaggcatttt
    18901 gaagaattac aaaaacatac agacttgata ttattagata taaaacatat tgataatgac
    18961 aaacatatta gattgacagg aaagcctaat acacacatcc ttaacttcgc gcgcaaactg
    19021 tcagatatga aacaacctgt atggattcga catgtccttg tgcctggtta ttctgatgat
    19081 aaagacgatt taattaaact aggggaattc attaattctc ttgataacgt cgaaaagttt
    19141 gaaattctgc catatcatca gttaggtgtt cataagtgga aaacattggg cattgcatat
    19201 gaattagaag atgtcgaagc gcccgatgat gaagctgtta aagcagccta ccgttatgtt
    19261 aacttcaaag ggaaaattcc cgttgaatta taaatacaat tcagaccgaa aagaaagcat
    19321 atgcaacttc aagagtgaag gggcatatgc ttctttttca attgagtatt gagtattagc
    19381 aagacgtagt aagtatatga gacaagtcct acaatggttg aaggaatacg tttttgtaag
    19441 tagctatgct gataaagaat gtgatgcctt gttaaaggtg gggttccaat atcatcgaat
    19501 gggttattaa ttgctacttg catatgaata tgagtctttt caaattttta ttgaccctga
    19561 gtaatgaaaa atattaagat gaaacttaat attaaagcaa tgcggagcgt gattatgaag
    19621 agaattagta aagatatatg ggcagtattt aaattactgt atcaaaataa agggcgtttt
    19681 agcattaatg ccttactatt gcagttaatc atgattttta ttagtagtac atacttaatt
    19741 ttactattta atatgatgtt aaaagtagct gggcaaagcc aacttacgat taacaattgg
    19801 atggaaatca taagtcatcc tgccagtgtg atacttctta ttatattcat attaagtgtt
    19861 gcctttctga tttatgtaga gttttcattg ttagtttata tggtttatgc cggctttgat
    19921 cgacagatta ttacatttaa atccattttt aaaaatgcct ttgtaaatgt gcgtaaactc
    19981 ataggtgtac cagttatttt ctttgtcatt tatttaatgt taatgatacc cattgccaac
    20041 ctaggactaa gttcagtatt aacaaaaaat atttacatac ctaaattttt aacggaagaa
    20101 cttatgaaaa cgacgaaagg tataatcatt tacggtacct ttatgattgc tgtatttata
    20161 ttaaacttta aattaatatt taccttaccg ttaacgattt taaaccgcca gtcgttattt
    20221 aaaaatatga gactaagttg gcaaattacg aagcgaaata agtttcgact tgttatagaa
    20281 atagttatat tggaactcat cattggtgcg attttaacat taattatttt aggagcaaca
    20341 tatcttgcta tttgtgtaga tgaagaagga gataagtttt tagtctcatc aattttattt
    20401 gttgtattga aaagcgcatt gttcttctat tatttattta cgaaattatc attaatcagt
    20461 gtgttagtac tgcacttaaa acaagagaat gtattagacc aaccgggctt agaatttaaa
    20521 tatccaaaac cgaaacggaa gtctaggttc tttataattt caatggtgct tgcagtgaca
    20581 tgttttatcg gttataacat gtacttactt tacaataata ctatcaatac aaatatctcc
    20641 attattggcc atcgtggttt cgaagataaa ggggttgaaa attctattcc gtcattgaaa
    20701 gctgctgcaa aagcgaatgt cgaatacgtt gagttagata caattatgac gaaagataaa
    20761 caatttgttg ttagtcatga taacaatttg aaacgtttaa caggtgttaa taaaaacatt
    20821 tctgaatcta atttcaaaga tgtcgtcggt ttgaaaatgc gtcaaaatgg acatgaagca
    20881 aaacttgtat ccttagacga atttattgaa acggctaaac aatcaaatgt gaagctacta
    20941 gttgagttaa agccacatgg taaagaacca gcagattata cacaacgtgt tattgatatt
    21001 ttgaaaaagc atggtgttga acatcaatat cgtgtgatgt ctttggatta tgatgtgatg
    21061 actaagttga aaaaagaagc gccatatctc aagtgtggtt atatcattcc gttgcagttt
    21121 ggtcatttta aagaaacatc attagatttc tttgtcatcg aagatttttc ttattcgcca
    21181 agacttgtta atcaagcgca cttggaaaat aaagaagtct atacttggac tattaacggc
    21241 gaagaagatt taacgaaata cttacaaacc aatgttgatg gtattatcac agatgaccca
    21301 gcattagctg atcagattaa agaagaaaag aaagacgaaa catacttcga tcgttctata
    21361 agaattttgt ttgaataata taaacaaaga cctctaaagt tatcaagatg ataccttcag
    21421 aggtcttttt aatgttgcca tctatgggat aggcaatcgt ttcattcgtt tatattcata
    21481 tgacaagtat ttgtatggca atttggcgtc acaaacactt acatgattta ttggtgaatt
    21541 attaattgtt ttgtgaatgc aaagggttag aaattgaatt gtaaatactt tctaatcttt
    21601 gtttcgcttt agtcatttga tccaaatttt tagtgcgtat agcggatttt gcaatatagt
    21661 gcgcagctaa aatatcgcgt ttttgaaacg catctaaatt taggtacgat aatttattta
    21721 agtcagtgtt tgctattaat tcatgtaatt gatctacaag cgcttgatgt tgatacgtat
    21781 gtgatgtagt ttcagatttg cttgctaatt taataccagt cgtatcaagg agcgccgctt
    21841 taataccagc aactaaatat gttttgattt tcatttgtgt tgtcatgctt tgttactcct
    21901 ttgatgtaca ttaatcaaaa aaattataca ctattgtata ttgcaaagct aattaactat
    21961 aacaaaaaga tagttaatgc tttgtttatt ctagttaata tatagttaat gtcttttaat
    22021 attttgtttc tttaatgtag attgggcaat tacattttgg aggaattaaa aaattatgaa
    22081 aaagcaaata atttcgctag gcgcattagc agttgcatct agcttattta catgggataa
    22141 caaagcagat gcgatagtaa caaaggatta tagtaaagaa tcaagagtga atgagaaaag
    22201 taaaaaggga gctactgttt cagattatta ctattggaaa ataattgata gtttagaggc
    22261 acaatttact ggagcaatag acttattgga agattataaa tatggagatc ctatctataa
    22321 agaagcgaaa gatagattga tgacaagagt attaggagaa gaccagtatt tattaaagaa
    22381 aaagattgat gaatatgagc tttataaaaa gtggtataaa agttcaaata agaacactaa
    22441 tatgcttact ttccataaat ataatcttta caatttaaca atgaatgaat ataacgatat
    22501 ttttaactct ttgaaagatg cagtttatca atttaataaa gaagttaaag aaatagagca
    22561 taaaaatgtt gacttgaagc agtttgataa agatggagaa gacaaggcaa ctaaagaagt
    22621 ttatgacctt gtttctgaaa ttgatacatt agttgtaact tattatgctg ataaggatta
    22681 tggggagcat gcgaaagagt tacgagcaaa actggactta atccttggag atacagacaa
    22741 tccacataaa attacaaatg agcgtataaa aaaagaaatg atcgatgact taaattcaat
    22801 tatagatgat ttctttatgg agactaaaca aaatagaccg aattctataa caaaatatga
    22861 tccaacaaaa cacaatttta aagagaagag tgaaaataaa cctaattttg ataaattagt
    22921 tgaagaaaca aaaaaagcag ttaaagaagc agacgaatct tggaaaaata aaactgtcaa
    22981 aaaatacgag gaaactgtaa caaaatctcc tgttgtaaaa gaagagaaga aagttgaaga
    23041 acctcaatta cctaaagttg gaaaccagca agaggttaaa actacggctg gtaaagctga
    23101 agaaacaaca caaccagtgg cacagccatt agtaaaaatt ccacaagaaa caatctatgg
    23161 tgaaactgta aaaggtccag aatatccaac gatggaaaat aaaacgttac aaggtgaaat
    23221 cgttcaaggt cccgattttc taacaatgga acaaaacaga ccatctttaa gcgataatta
    23281 tactcaaccg acgacaccga accctatttt agaaggtctt gaaggtagct catctaaact
    23341 tgaaataaaa ccacaaggta ctgaatcaac gttgaaaggt attcaaggag aatcaagtga
    23401 tattgaagtt aaacctcaag caactgaaac aacagaagct tctcaatatg gtccgagacc
    23461 gcaatttaac aaaacaccta agtatgtgaa atatagagat gctggtacag gtatccgtga
    23521 atacaacgat ggaacatttg gatatgaagc gagaccaaga ttcaacaagc caagtgaaac
    23581 aaatgcatac aacgtaacga caaatcaaga tggcacagta tcatacggag ctcgcccaac
    23641 acaaaacaag ccaagtgaaa caaacgcata taacgtaaca acacatgcaa atggtcaagt
    23701 atcatacggt gctcgcccaa cacaaaacaa gccaagcaaa acaaatgcat acaacgtaac
    23761 aacacatgca aatggtcaag tatcatatgg cgctcgcccg acacaaaaaa agccaagcaa
    23821 aacaaatgca tataacgtaa caacacatgc aaatggtcaa gtatcatacg gagctcgccc
    23881 gacatacaag aagccaagcg aaacaaatgc atacaacgta acaacacatg caaatggtca
    23941 agtatcatat ggcgctcgcc cgacacaaaa aaagccaagc gaaacaaacg catataacgt
    24001 aacaacacat gcagatggta ctgcgacata tgggcctaga gtaacaaaat aaatttataa
    24061 ctctatccaa agacatacag tcaatacaaa gaattatgta tctatacaac agtaatcatg
    24121 cattctatga tgcttctaac tgaattaaag catcgaacaa tcggaagcat atttctaaat
    24181 tatttattca ttatagtctt aaacataaca tgacctaata tattactaac ctattaaaat
    24241 aaaccacgca catctaagtg atatacgaca atcacagcaa taataattgc tttagaaagt
    24301 cgtaccgaac tggaacttac aagtctagtt cgaacacaca ctgatgtgag tggttttctt
    24361 tattttaaac atgaacaatc agataagtta ctagcattag caaatattat taaatcaaag
    24421 ggcttcgatt cataaaattt aaaacaatga ttaaaattag acgtgtaaat gttaaattct
    24481 aaaacggaaa taaccaccat cccattaaac cacttttttg ttcaatcact atatttcaca
    24541 cagcttcatt aataaaacga aattgcttca acccgcttca acttcagcct acttcattca
    24601 ataacctaac gaatccgctt catccaaaat caaccattct aacgcacata ctcaaatata
    24661 gcagctgcac ccatgccgac accaatacac atcgtaacca tgccgtaacg gctatcggga
    24721 cgtctaccca tttcattaag taaacgcgcg gttaacattg cccctgtagc acctaatgga
    24781 tgacctaaag caatagcgcc accattcaca ttcgtacgtg atatatctag acctacttct
    24841 ttaatagatg caatcgtttg agaagcaaat gcttcgttca attcgatcaa atcaatgtct
    24901 tcaacagata gattgctgag tgacaatact tcaggaatcg catatgcagg cccaataccc
    24961 ataattttcg ggtcaacgcc tactgcctta taaccaacga atcgtgcaat aggtgtcacg
    25021 cctagttctt tcactttatc tccagacatt aaaactacaa atcctgcacc atcagaaagt
    25081 ggggcagatg ttcccgcagt cacagtgccg tcagctttaa atactgtacg taatttggct
    25141 aatgcctcca tcgtggtgtc agggcgtata aattcatctt ggtcaaagat atttgtgtgt
    25201 acttttggtc ctgcgtttgt atattcaact gagtttaccc gtattggaat aatttcatct
    25261 ttgaaccgac catcacgttg tgcgtcatag gcacgttgat gacttctgac agcataagca
    25321 tcttgatctt cgcgtgatac gtcaaattgg gatgctacat tttcagcagt taaacccata
    25381 ggatatgacg cacctatatc atcatattgt aaggttggat tgtttgtggg ctcgttgcca
    25441 cccattggta cggcactcat caattcaacg ccaccagcta caagtatatc tccttgacca
    25501 gccataattt gattggctgc aatcgcgatg gtttgtaatc ctgatgagca gtagcgattc
    25561 actgtttgac ccggtaccgt gtcagataat cccgcacgca atgcaatcgt tcgtgcaatg
    25621 ttttgtcctt gtaatccttc tggaaaagcc gtaccaacaa tgacatcttc aatcatattc
    25681 ttattgaatt ttccgtcaat acgtttcaat acgccttgta atactttggc tgcgacatca
    25741 tcaggtcttt cgtggaataa tgcgccttgc tttgctttcg ctgcggctga acgcccataa
    25801 gctacaatgt atgcttcttg catggttatc atcctctctt aatgactatc ttttaattac
    25861 gtaatggctt accagttttt aacatatgtg caattctttc atatgatttt ttagatttta
    25921 gtaagtcaat aaagccaatt ttctccaacg attgaatgta acgttgattt ataaatgtat
    25981 ttcttggtaa atcaccaccc gctaaaattg tggcgatatt taaggcgata tgataatcat
    26041 ggtcgctaat aaaatgcccc cgtctttgcg catctaattg tccttggatc aatgctttga
    26101 agtcttcacc taaagcgata tattgatgtc taggattcgg aatatagttt gtttctgctt
    26161 catatttcgc acgtttgagc gcaacttcga cacgttgtgc tgtattgaaa ataatcgtat
    26221 ctgtatcacg taaataacca taacgacgtg cctcaaaggc atttgtagag actttcgcaa
    26281 atgcgatatt cgtcagtact tttgtcatgg aagcttgttt gtcatcaaac ttatgcgatg
    26341 tgcgtaatat gcgatcagcc atttctgcaa ggccaccgcc actcggtaat aagccaacgc
    26401 ctgcttcaac aagacctata tatgtttcac ttgcagcgac aacaataggt gagtaaagta
    26461 caagctcaca gccaccgcct aaggcacgac cttgaacagc tgtgactact ggtttcaaac
    26521 tatacttcaa acgattaaag ctataatgta atttatcaat tgattgtgca acgacatcat
    26581 ctacaagacc gtcttcatgc gcctttttca ttaagaaaag gttagcaccc acactgaaat
    26641 tattaccatc tgcataaatg accatacttg tgtaatggtc attttccagt aaatcaatgg
    26701 catcaactaa cgcatcgttg aattcatcgg taatgacatt atttttactt tgtaatttca
    26761 gtaacagttg atcatcatga gttacggaaa gtttggcatc acctttatcc caaagttcat
    26821 cttttacgaa gtgagaaaca ggtgttgcat attcaatggt ctcatcttgt ttataaaagc
    26881 caccatctaa atcactaatc cattgtggta agtctccaag ttcgtcttcc atacgtgttt
    26941 taacacgttc gtatcccatt gcatcccata attggaatgg accaagtttc cagttgaacc
    27001 cccagacaag cgcacggtct atgtctcgga aatcatcggt agctttaggt acattgatag
    27061 cagagtaata gaaattatta cgtaatgtct cccataaaaa tagtcccgct tcgtcttgcg
    27121 cattgaatat gacatcaagg ttatgcacta agtctttatt aaattcattt aaaattggta
    27181 attgtggttg cgatacaggt acataatctt gtttttcaac atcgtaaaca agtcgagctt
    27241 tagtttcttt atcctttttg taaaatcctt gtttcgtttt acgtccgagt gcgccattgt
    27301 caaacaacgt atttacaatt ttgacatcat gaaaataagg cgtttcttca ggtacttgtt
    27361 gcatgccttt aattacagac actgcaatat ctaaaccgac taggtcggat agggcatatg
    27421 tacctgtttt aggacgacca atcgcttgcc cagttaaagc atccacatct acaatgctta
    27481 acttgtgttg ctcggcgcga tacataatat cattcattgt ttgcgtgccg actctatttg
    27541 cgacaaagcc aggcacatca ttgacgacaa tgacaccttt acctaataca ttttgcgcga
    27601 aattttttac atctaatatg atagattcct tcgtgtgtga cgtaggtatt aactccacta
    27661 atttcataat acgtggtggg ttaaagaaat gtagaccaaa gaatcgttct tgatccttct
    27721 cgttaaatgc ttgagcaatc gcattaattg gaatacctga tgtatttgta gcaaataaag
    27781 catcttcttt agcatgttgt agaacttgtt gccaaacagc atgcttaatt tcaatatctt
    27841 ctttgactgc ttcgatatat aaatcagcat catcatttac caagtcatca tcaaaattac
    27901 catatgttaa atgactcact agatttaagt cgaatagtag cggccgtttc ttatctgtaa
    27961 ttttatcgta agattttttc gcaatgagat ttggatcgtt tttgtccact acaatatcta
    28021 atagttttac tttaagtcca gcattcacaa agagtgctgc cagttgagcg cccatcgtgc
    28081 ctgcgccaag aacggttact ttattaattg tcatagtgat tcctccaatt tagttgagga
    28141 taagataacc attaagataa ttggaataac gttgctattt tataaaatta attaagtatc
    28201 tttgacagtc atcttagcct cttatttaag gaaaaagctt tatgcttaaa ataagtcttt
    28261 tttagtgaaa ttaatgcatc tcatataatt atttgctatt tatacgaaag cagaatctcc
    28321 agtcaaagcg cgtccaatta ctaaggcatt aatttcatgt gtaccttcgt acgtgtaaat
    28381 cgcttctgca tcagagaaga aacgtgcaat atcataatcg tcagctagta tgccattacc
    28441 acctgtaata ccgcggccca tagctactgt ctcacgcaaa cgtaaggcat tcatcatctt
    28501 cgccgttgaa gttgcaacct cgtcatattc accatgtgct tgcatattag ctaattgagc
    28561 acatgttgcc attgcttgag ctaaattacc ttgcatcatt gctagctttt cttgtattaa
    28621 ctgatattta ctaattggtt tgccgaattg cttacgctca gtgacataat ctaatgtggc
    28681 acgtaaagcg ccagccatac cacctgtagc catataagca acgcctgctc tcgttgaata
    28741 aagaattttg gcaatatctt taaagcttgt tatgttttgt aagcgatccg cttcatctac
    28801 tttgacatta gttaatttaa ttagtgcgtt aggaacaatg cgaagtgcga ttttattatc
    28861 aatgacttca atatcgacgc catcttgttc tggtctgact acaaagcaat ggggtttgcc
    28921 agttttttta tttactgcga atactggaat gacatcagat acatgtgcac caccaatcca
    28981 tttcttttca ccattgataa cccaagtatc gccttggcgt tcagcgactg tttcaagacc
    29041 tcccgcaacg tccgaaccgt gttctggttc agttaaagca aagcatgtac gcagttcatg
    29101 tgactgtaat ttaggtacat atttcgcaat ttgttctttg ctacctccga aatagaaagt
    29161 gttatgccct aaaccttggt gaacaccgag tagggtagct aaggaaatat caaatcgcgc
    29221 gagtaggtaa gacatgaaaa actgaaatag ttgactaggc attttggcgt ttggacgatc
    29281 cttgtaaagt aatggattgt taaaataatt taattctccc agatctttaa aatagtcctc
    29341 gggtacagta gcgtctatcc aatgttgatt aatattttca cggtacttac tttctagcaa
    29401 tgaatctact tgttgtaaaa attcgacttc accgtctgtt aaacctttag caatactaag
    29461 tacatcttca ggaaataatg tttttaagac cgtttctttt tcaaatgtca tataaattcc
    29521 tcctaaaaat aatatgaata ctaatgtgaa atgcatttaa ttcaaaaaca acacgcttta
    29581 tttgtaaacg cttacactaa atgtcaaaaa tttttatcac ctttaaagtg tttgtgagac
    29641 tttgtcattc atcatttgtc gaatcgcaag tttatctggt ttctgcgtac tgtttaacgg
    29701 catatgtgtc actggtacat acattcttgg gactttataa cctgctaaac gacttcgcat
    29761 atgttgatct aaaatttcag cgtaatgagg ttcatcttcg cgaagtataa tggctgcagc
    29821 aattgattca ccatattttg gatgatcata gccaacgacc acacaccggt ctactagtgg
    29881 atgctcagct aaagcatttt cgacttcaga tggtaagaca ttttcgccac cagttatgat
    29941 taattctttt ttgcggtcaa taataaatat atcgccatcg tcgtccatct tcgctaagtc
    30001 accagttaat aaataacgac catgaaatgc tttggcagtc tctgctggtt tattccaata
    30061 tcctggcgtg acatttttag ccttaattgc aagttcgcca atctcaccag taggtacttc
    30121 ctcaccgtta tcatcaagga tacgtgcatc aacgaacatg actgctttac caatactcat
    30181 tggcttacgt tttgaatttt ccggtgtatt aacaagtaca agaggtgctt cagttaaacc
    30241 atagccgtta ataatgttta tgccatattg tttaaaagct gcttggatac ttggtaatgg
    30301 ttgtgaacca ccttggatga tataatccat agctctaaaa ttttcaggat taaaattact
    30361 agcacgtagc gtactataat acattgtcgg aatcatgata ataaatgtag ggtgatattg
    30421 tgcaatcatg tcattcaatt cttcgccgtt aaagtaacgt tgaagaataa gtgtgccacc
    30481 tgacattaat actggtaata cagtatcgtt aaaccctaaa acatggaaca ttggtgttga
    30541 tacaatcgta atatagtttg aattgaactt gtacgtcagc tctaagtttg caccgttatg
    30601 aacaaatgat tcatatgaga acatcacacc tttaggtgat ccggttgtac cacttgtata
    30661 aattaatgct gcaagatctt gtggttcaac aggtgttgct tgaaaaggtt ggtgataatc
    30721 tggatttacg atttcatcat attgcgccac atcaatatcc atatgcaata agttttggtc
    30781 aatatcggtg agtgaactta aatgtttttc agcatagaag agcagtttta attgtgcatc
    30841 ttccacaatg gctgcaattt cttttgggtt aagccgccaa ttcaatggta aaaaaaccgc
    30901 acctgtttta aaacaagcaa acaataaatc taatattgca atatcatttg gcgcaaaaat
    30961 accgataaca tcgccttttt taacaccttg agatgttaaa taatgtgcca tattatcagc
    31021 gcgtgcattg agttgttggt atgtccaaga tgtttgtttt gcgtgatcaa taacggcagg
    31081 cttgtcatca tcgaagtctg aacgcgtttt tatccaatcg aaattcatta gtataccccc
    31141 tttagcttca ctttcatact ttatgaattg attgtttaag ttgtccccat ttttctttgt
    31201 aaatgctggt atcaattaat tttaaatgat cagcaataat tggtttaaaa gccatttgat
    31261 tcaaaatatc tttatgcaaa tcaagacctg gtgcaatttc aattagtttc aagccttgat
    31321 tggtgagctc gaatactgca cgatcagtaa caaaatagac ttcttgctcg agtaattgtg
    31381 aatattgtgc attaaagtcg atatggctca catctgatac aaatttctgg ttttgtcctt
    31441 cagtttcaat gtttaatcgt tgattatgac atgagacatg actgccagct acaaaagtac
    31501 ctgaaaagat aattttattt acagattgcg taatgtctat aaagccacca catccattta
    31561 gtcggtcatt gaagtaagac acgttgacat tgccgtattg atcaacctca gcaaagctaa
    31621 gataggcaac tgatacacca ttgttataaa taaaatccca tgctcgatca tgaggcatgc
    31681 gcacatctgc attgtaattc ataccaaaat gttcacgact cccaacgaat ccaccgaaaa
    31741 tgccaacatc tagaatcggt tgcacatcat gttcaacaca ttcttcgtgc aataaattag
    31801 agagttcatt attgatgcca taaccgatgc taattgtatc gccataagtt aaaaactgag
    31861 cagcacgtcg gagaatcaat ttgcgactat taaaaggtaa tgcgggttca ggtattccat
    31921 caattcgttc ttctccagac aaggctggta aataatgact ctgaattact tggcggtgat
    31981 tcttttcatc ttctgtgacg tatacataat cgacaagatt tcctgggata acaacttcat
    32041 tcggttttag ttgatagtcg tcaactaaag ctttaacttg tacaataact ttcccatgat
    32101 tggctttcgc gtttaatgcg acatgataac actcgctcaa gtacgcttct tgagttaaat
    32161 aaatgttacc ttgttgatct gcgtatgttc ctctcagtag tgccacatca acgctaggga
    32221 atgtgtaatg taagtatgtt tcatcgttga tggttactaa tgaaactaaa tcatcagttg
    32281 ttcgtgtatt tactttaccg ccaccgtatc taggatcaac agctgtgttt aatccgattt
    32341 tagtaataac tccaggtaat aattgattac tctgacgata atgagttgca atgatacctt
    32401 gtggtaaaaa ataagcttca atgtcattat ttttcattgc ttgtgccgtt ttggaagaag
    32461 ccgttaaaat gctcataatg acacgtttaa tcatgcgacg ttctataaaa tcatctaaat
    32521 ccggtgcggc acctaaacta tggatatcat tcgctaatat aaacgttaaa tctttgggcg
    32581 tatgatatgt gtcatgttgc gctaacacag cacgtagaac ttcggcgggt aagttggcta
    32641 cagctaatgc tggtaaacca atcacatcac catctttaat gatatgttgt aagtcgtgcc
    32701 atgtgatttg tttcaagcaa gtcacctcca tcacatttga taaaatatag cgtttttaca
    32761 ctttgtgtaa acccttacaa gaaatataac ataacgacgt ttaaaatcaa ttagaaatat
    32821 ctttttattc tgataataga cgcagtatag acacatttta atggttgata acaattgtaa
    32881 tatcaagggt ttgtaatgaa ttgaatatca ttaaaatact tatataaaaa tattgctcg
//
LOCUS       DAOBBH010000008        32708 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.008, whole genome shotgun sequence.
ACCESSION   DAOBBH010000008 DAOBBH010000000
VERSION     DAOBBH010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 32708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32708
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.008"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            345..1604
                     /locus_tag="P6L01_000229"
     CDS             345..1604
                     /locus_tag="P6L01_000229"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDP3029330.1"
                     /translation="MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLE
                     GLPQISDVKTLVSLLEDLNIKASLNGTELEVDTTEIQNAALPNNKVESLRASYYMMGA
                     MLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIF
                     LDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKI
                     NGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNV
                     DVRDERIRINNNAPYQFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERF
                     KHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYN
                     VKHIYRGYTDIVEHLKALGADIWTETV"
     gene            1693..2028
                     /locus_tag="P6L01_000230"
     CDS             1693..2028
                     /locus_tag="P6L01_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDP3029331.1"
                     /translation="MEVCPYLEETFKILGRSWNGLIINYLSRCNDCSAHFSDMKRDLK
                     TITPRALSLKLSELAQWELVEKQIISTSPVQIIYVLTEKGKALAEALHPIEAWAQSYV
                     DLTDQRTAK"
     gene            2266..3693
                     /locus_tag="P6L01_000231"
     CDS             2266..3693
                     /locus_tag="P6L01_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDP3029332.1"
                     /translation="MRDYTKQYINGEWVESNSNETIEVINPATEEVIGKVAKGNKADV
                     DKAVEAADDVYLEFRHTSVKERQALLDKIVKEYENRKDDIVQAITDELGAPLSLSERV
                     HYQMGLNHFVAARDALDNYEFEERRGDDLVVKEAIGVSGLITPWNFPTNQTSLKLAAA
                     FAAGSPVVLKPSEETPFAAVILAEIFDKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRM
                     MSFTGSGPTGSKIMEKAAKDFKKVSLELGGKSPYIVLDDVDIKEAAKATTGKVVNNTG
                     QVCTAGTRVLVPNKIKDAFLAELKEQFSQVRVGNPREDGTQVGPIISKKQFDQVQNYI
                     NKGIEEGAELFYGGPGKPEGLEKGYFARPTIFINVDNQMTIAQEEIFGPVMSVITYND
                     LDEAIQIANDTKYGLAGYVIGKDKETLHKVARSIEAGTVEINEAGRKPDLPFGGYKQS
                     GLGREWGDYGIEEFLEVKSIAGYFK"
     gene            3940..5256
                     /gene="rho"
                     /locus_tag="P6L01_000232"
     CDS             3940..5256
                     /gene="rho"
                     /locus_tag="P6L01_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDP3029333.1"
                     /translation="MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKK
                     ELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEI
                     KRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLET
                     ETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVG
                     ERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSI
                     TRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGS
                     RMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQL
                     RNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII"
     gene            5374..5628
                     /locus_tag="P6L01_000233"
     CDS             5374..5628
                     /locus_tag="P6L01_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDP3029334.1"
                     /translation="MKQGIHPEYHQVIFLDTTTNFKFLSGSTKTSSEMMEWEDGKEYP
                     VIRLDISSDSHPFYTGRQKFAAADGRVERFNKKFGLKSNN"
     gene            5975..6574
                     /locus_tag="P6L01_000234"
     CDS             5975..6574
                     /locus_tag="P6L01_000234"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDP3029335.1"
                     /translation="MYETYHSGWIECITGSMFSGKSEELIRRLRRGIYAKQKVVVFKP
                     AIDDRYHKEKVVSHNGNAIEAINISKASEIMTHNLTNVDVIGIDEVQFFDDEIVSIVE
                     KLSADGHRVIVAGLDMDFRGEPFEPMPKLMAVSEQVTKLQAVCAVCGSSSSRTQRLIN
                     GKPAKIDDPIILVGANESYEPRCRAHHIVAPSDNNKEEL"
     gene            6575..7651
                     /gene="prfA"
                     /locus_tag="P6L01_000235"
     CDS             6575..7651
                     /gene="prfA"
                     /locus_tag="P6L01_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDP3029336.1"
                     /translation="MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKT
                     VDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEESNGIKAELPNLEEELKILLIPK
                     DPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
                     ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNE
                     DLKIDTYRSSGAGGQHVNTTDSAVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLY
                     DMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHL
                     EEIIDALTLSEQTDKLKELNNGEL"
     gene            7638..8474
                     /gene="prmC"
                     /locus_tag="P6L01_000236"
     CDS             7638..8474
                     /gene="prmC"
                     /locus_tag="P6L01_000236"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDP3029337.1"
                     /translation="MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHM
                     HDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLH
                     FLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQI
                     QFLTGDALKPLIKEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAI
                     YESIIGDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSF
                     KW"
     gene            8558..9604
                     /locus_tag="P6L01_000237"
     CDS             8558..9604
                     /locus_tag="P6L01_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDP3029338.1"
                     /translation="MDTKIWDVREYNEDLQQYPKINEIKDIVLNGGLIGLPTETVYGL
                     AANATDEEAVAKIYEAKGRPSDNPLIVHIHSKVQLKDFTYTLDPRVEKLMQAFWPGPI
                     SFILPLKPGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTT
                     FNHVYQDLNARIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITEILPNSI
                     AHADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVAF
                     IPSEAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRM
                     LKAAGNHIIKGCEL"
     gene            9601..10020
                     /locus_tag="P6L01_000238"
     CDS             9601..10020
                     /locus_tag="P6L01_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HDP3029339.1"
                     /translation="MKILFVCTGNTCRSPLAESIAKEVMPNHQFESRGIFAVNNQGVS
                     NYVEDLVEEHHLAETTLSQQFTEADLKADIILTMSYSHKELIEAHFGLQNHVFTLHEY
                     VKEAGEVIDPYGGTKEMYVHTYEELVSLILKLKDIIC"
     gene            10127..10651
                     /locus_tag="P6L01_000239"
     CDS             10127..10651
                     /locus_tag="P6L01_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDP3029340.1"
                     /translation="MKDLTMLLDELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSME
                     VAETIFNALDVVSKETGVTFAFQGCEHINRAITIEKSQYNPLTMEEVSVVPDVHAGGS
                     LATYAFQHMKDPIVVEHITVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIAT
                     SRPKKIGGERAKYK"
     gene            10678..11916
                     /locus_tag="P6L01_000240"
     CDS             10678..11916
                     /locus_tag="P6L01_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDP3029341.1"
                     /translation="MSYITKQDKVIAEAIEREFQRQNSNIELIASENFVSEAVMEAQG
                     SVLTNKYAEGYPGRRYYGGCEFVDVTESIAIDRAKALFGAEHVNVQPHSGSQANMAVY
                     LVALEMGDTVLGMNLSHGGHLTHGAPVNFSGKFYNFVEYGVDKDTERINYDEVRKLAL
                     EHKPKLIVAGASAYSRTIDFKKFKEIADEVNAKLMVDMAHIAGLVAAGLHPNPVEYAD
                     FVTTTTHKTLRGPRGGMILCKEEYKKDIDKTIFPGIQGGPLEHVIAAKAVAFGEALEN
                     NFKTYQQQVVKNAKVLAEALINEGFRIVSGGTDNHLVAVDVKGSIGLTGKEAEETLDS
                     VGITCNKNTIPFDQEKPFVTSGIRLGTPAATTRGFDEKAFEEVAKIISLALKNSKDEE
                     KLQQAKERVAKLTAEYPLYQ"
     gene            11944..12573
                     /gene="upp"
                     /locus_tag="P6L01_000241"
     CDS             11944..12573
                     /gene="upp"
                     /locus_tag="P6L01_000241"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDP3029342.1"
                     /translation="MSKVHVFDHPLIQHKLSYIRDVNTGTKEFRELVDEVGMLMAYEV
                     TRDLELQDVDIETPVTKMTAKRLAGKKLAIVPILRAGLGMTDGILSLVPAARVGHIGL
                     YRDPETLKAVEYFAKLPQDITERQIIVVDPMLATGASAIEAITSLKKRGAKNIRFMCL
                     IAAPEGVEKMHEAHPDVDIYIAALDEKLNDKAYITPGLGDAGDRLFGTK"
     gene            12597..13724
                     /gene="wecB"
                     /locus_tag="P6L01_000242"
     CDS             12597..13724
                     /gene="wecB"
                     /locus_tag="P6L01_000242"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDP3029343.1"
                     /translation="MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREML
                     DSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFA
                     GGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSE
                     GKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIF
                     KAVRRLIDEYTDLALVYPMHKNPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSY
                     FILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDD
                     ERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDF"
     gene            13792..14244
                     /locus_tag="P6L01_000243"
     CDS             13792..14244
                     /locus_tag="P6L01_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDP3029344.1"
                     /translation="MNRIDTTNRLYYHLVCMSGTVFMIDISHTEAYKMSRFYNIFKQF
                     IQYYFYLIIILGGLYLYTHHAFILGLIIGVTGSAINTCIFECYLAKAKRPDTMHISTG
                     NMWRYLVAIIACMIWYLNKAHVSIIGIIIGLMISYVVVIIRPLLKVSK"
     gene            14265..14993
                     /gene="atpB"
                     /locus_tag="P6L01_000244"
     CDS             14265..14993
                     /gene="atpB"
                     /locus_tag="P6L01_000244"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDP3029345.1"
                     /translation="MDHKSPLVSWNLFGFDIVFNLSSILMILVTAFLVFLLAIICTRN
                     LKKRPTGKQNFVEWIFDFVRGIIEGNMAWKKGGQFHFLAVTLILYIFIANMLGLPFSI
                     VTKDHTLWWKSPTADATVTLTLSTTIILLTHFYGIKMRGTKQYLKGYVQPFWPLAIIN
                     VFEEFTSTLTLGLRLYGNIFAGEILLTLLAGLFFNEPAWGWIISIPGLIVWQAFSIFV
                     GTIQAYIFIMLSMVYMSHKVADEH"
     gene            15036..15248
                     /gene="atpE"
                     /locus_tag="P6L01_000245"
     CDS             15036..15248
                     /gene="atpE"
                     /locus_tag="P6L01_000245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDP3029346.1"
                     /translation="MNLIAAAIAIGLSALGAGIGNGLIVSRTVEGVARQPEARGQLMG
                     IMFIGVGLVEALPIIGVVIAFMTFAG"
     gene            15446..15967
                     /locus_tag="P6L01_000246"
     CDS             15446..15967
                     /locus_tag="P6L01_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDP3029347.1"
                     /translation="MTETANLFVLGAAGGVEWGTVIVQVLTFIVLLALLKKFAWGPLK
                     DVMDKRERDINRDIDDAEQAKLNAQKLEEENKQKLKETQEEVQKILEDAKVQARQQQE
                     QIIHEANVRANGMIETAQSEINSQKERAIADINNQVSELSVLIASKVLRKEISEQDPK
                     ALVDKYLKEAGDK"
     gene            15967..16506
                     /locus_tag="P6L01_000247"
     CDS             15967..16506
                     /locus_tag="P6L01_000247"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDP3029348.1"
                     /translation="MVKVANKYAKALFDVSLDTNNLETINEELTVINEAVKDKIEQLK
                     MVDSNPTQTAEQRRELINGVFTDINPYIKNMMYVLADNRHISLIADVFKAFQSLYNGH
                     YNQDFATIESTYELSQEELDKIVKLVTQQTKLSKVIVDTKINPDLIGGFRVKVGTTVL
                     DGSVRNDLVQLQRKFRRVN"
     gene            16528..18036
                     /locus_tag="P6L01_000248"
     CDS             16528..18036
                     /locus_tag="P6L01_000248"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDP3029349.1"
                     /translation="MAIKAEEISALLRSQIENYESEMSVTDVGTVLQIGDGIALIHGL
                     NDVMAGELVEFHNGVLGLAQNLEESNVGVVILGPYTGITEGDEVKRTGRIMEVPVGEE
                     LIGRVVNPLGQPIDGQGPINTTKTRPVEKKATGVMDRKSVDEPLQTGIKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQGTICIYVAIGQKDSTVRANVEKLRQAGAL
                     DYTIVVAASASEPSPLLYIAPYSGVTMGEEFMFNGKHVLIVYDDLTKQAAAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSITALPIIETQAGDISAYVPTNV
                     ISITDGQIFLQSDLFFSGVRPAINAGQSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     ESFAQFGSDLDEFTASKLERGKRTVEVLKQDQNKPLPVEHQVLIIYALTKGYLDDIPV
                     VDITRFEDELNHWAESNATELLNEIRETGGLPDAEKFDTAINEFKKSFSKSE"
     gene            18067..18933
                     /locus_tag="P6L01_000249"
     CDS             18067..18933
                     /locus_tag="P6L01_000249"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDP3029350.1"
                     /translation="MASLKEIDTRIKSTKKMKQITKAMNMVSSSKLRRAEKNTKQFTP
                     YMDKMQDAITAVAGASSNTNHPMLRPRKITRSGYLVITSDKGLAGAYSANVLKKLITD
                     IEAKHQDSSEYSIVVLGQQGVDFLKNRGYDIEYSQVDVPDQPSFKSVQALANHAIDLY
                     SEEEIDELNIYYSHYVSVLENKPTSRQVLPLSQEDSSKGHGHLSSYEFEPDKESILSV
                     ILPQYVESLIYGTILDAKASEHATRMTAMKNATDNATELIDDLSLEYNRARQAEITQQ
                     ITEIVGGSAALE"
     gene            18955..20367
                     /gene="atpD"
                     /locus_tag="P6L01_000250"
     CDS             18955..20367
                     /gene="atpD"
                     /locus_tag="P6L01_000250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDP3029351.1"
                     /translation="MGIGRVTQVMGPVIDVRFEHNEVPKINNALVIDVPKEEGTIQLT
                     LEVALQLGDDVVRTIAMDSTDGVQRGMDVKDTGKEISVPVGDETLGRVFNVLGETIDL
                     KEEISDSVRRDPIHRQAPAFDELSTEVQILETGIKVVDLLAPYIKGGKIGLFGGAGVG
                     KTVLIQELINNIAQEHGGISVFAGVGERTREGNDLYFEMSDSGVIKKTAMVFGQMNEP
                     PGARMRVALSGLTMAEYFRDEQGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTTKGSVTSIQAVFVPADDYTDPAPATAFAHLDATTNLERKLTE
                     MGIYPAVDPLASTSRALEPSIVGQEHYEVARDVQSTLQKYRELQDIIAILGMDELSDE
                     DKQTVERARRIQFFLSQNFHVAEQFTGQKGSYVPVKTTVANFKDILDGKYDHIPEDAF
                     RLVGSMDDVIAKAKDMGVEV"
     gene            20387..20791
                     /locus_tag="P6L01_000251"
     CDS             20387..20791
                     /locus_tag="P6L01_000251"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDP3029352.1"
                     /translation="MNTLNLDIVTPNGSVYNRDNVELVVMQTTAGEIGVMSGHIPTVA
                     ALKTGFVKVKFHDGTEYIAVSDGFVEVRKDKVSIIVQTAETAREIDVERAKLAKARAE
                     SHLENDDDNTDIHRAERALERANNRLRVAELK"
     gene            21129..21221
                     /locus_tag="P6L01_000252"
     CDS             21129..21221
                     /locus_tag="P6L01_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029353.1"
                     /translation="MLKSKFPSPLSTVDISFFEIIYIFSYQDIL"
     gene            21353..21586
                     /locus_tag="P6L01_000253"
     CDS             21353..21586
                     /locus_tag="P6L01_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDP3029354.1"
                     /translation="MDYIGQYAVIHLVLHVVCICIAYWALQSIRLDQFFKKGYATQLQ
                     VCMIFVAILLGTAVSNFIVDLLQYSTQVKYLIK"
     gene            21697..22962
                     /gene="murA"
                     /locus_tag="P6L01_000254"
     CDS             21697..22962
                     /gene="murA"
                     /locus_tag="P6L01_000254"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDP3029355.1"
                     /translation="MDKIVIKGGNKLTGEVKVEGAKNAVLPILTASLLASDKPSKLVN
                     VPALSDVETINNVLTTLNADVTYKKDENAVVVDATKTLNEEAPYEYVSKMRASILVMG
                     PLLARLGHAIVALPGGCAIGSRPIEQHIKGFEALGAEIHLENGNIYANAKDGLKGTSI
                     HLDFPSVGATQNIIMAASLAKGKTLIENAAKEPEIVDLANYINEMGGRITGAGTDTIT
                     INGVESLHGVEHAIIPDRIEAGTLLIAGAITRGDIFVRGAIKEHMASLVYKLEEMGVE
                     LDYQEDGIRVRAEGELQPVDIKTLPHPGFPTDMQSQMMALLLTANGHKVVTETVFENR
                     FMHVAEFKRMNANINVEGRSAKLEGKSQLQGAQVKATDLRAAAALILAGLVADGKTSV
                     TELTHLDRGYVDLHGKLKQLGADIERIND"
     gene            22996..23436
                     /gene="fabZ"
                     /locus_tag="P6L01_000255"
     CDS             22996..23436
                     /gene="fabZ"
                     /locus_tag="P6L01_000255"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDP3029356.1"
                     /translation="METIFDYNQIKQIIPHRQPFLLIDKVVEYEEGQRCVAIKQVSGN
                     EPFFQGHFPEYAVMPGVLITEALAQTGAVAILNSEENKGKIALFAGIDKCRFKRQVVP
                     GDTLTLEVEITKIKGPIGKGNAKATVDGQLACSCELTFAIQDVK"
     gene            complement(23495..23935)
                     /locus_tag="P6L01_000256"
     CDS             complement(23495..23935)
                     /locus_tag="P6L01_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwpF-like family protein"
                     /protein_id="HDP3029357.1"
                     /translation="MKTFKAVRFQIVNEHGRIIEYELEDGVIINKEESGTGWLLEIVI
                     SNEHYETFKEYQDNEQLLDIRVVITRPANDPALFESTVKSIKNFKTTMSIVFECHIYT
                     LRQQYAESLLEQLIDDGLSGEELKKSFNRMMQSKPKLKDEKLEE"
     gene            24129..24524
                     /locus_tag="P6L01_000257"
     CDS             24129..24524
                     /locus_tag="P6L01_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDP3029358.1"
                     /translation="MLNKIVIVGRLTKDAQIFEKEDRKIATFCVATHRNYKDENGEIV
                     CDYLFCKAFGKLASNIEKYTNQGTLVGITGQMRSRKYDKDGQTHFVTELYVETIKFMS
                     PKSQNNEILSDSILDIDSQNIDNHDLLEI"
     gene            24913..25608
                     /gene="sceD"
                     /locus_tag="P6L01_000258"
     CDS             24913..25608
                     /gene="sceD"
                     /locus_tag="P6L01_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase SceD"
                     /protein_id="HDP3029359.1"
                     /translation="MKKTLLASSLAVGLGIVAGNAGHEAHASEADLNKASLAQMAQSN
                     DQTLNQKPIEAGAYNYTFDYEGFTYHFESDGTHFAWNYHATGANGANMSAQAPATNNV
                     EPSAVQANQVQSQEVEAPQNAQTQQPQASTSNNSQVTATPTESKASEGSSVNVNAHLK
                     QIAQRESGGNIHAVNPTSGAAGKYQFLQSTWDSVAPAKYKGVSPANAPESVQDAAAVK
                     LYNTGGAGHWVTA"
     regulatory      25813..25915
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            25995..26684
                     /gene="tenA"
                     /locus_tag="P6L01_000259"
     CDS             25995..26684
                     /gene="tenA"
                     /locus_tag="P6L01_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HDP3029360.1"
                     /translation="MEFSQKLYQAAKPIINDIYEDDFIQKMLSGDIGADALRHYLKAD
                     AAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVEGEVLAHDILAQIVGESYEEIIK
                     TKVWPPSGDHYIKHMYFQAHSRENAIYTIAAMAPCPYIYAELAKRSQSDHKLNREKDT
                     AKWFDFYSTEMDDIINVFEALMNKLAESMSDKELEQVKQVFLESCIHERRFFNMAMTL
                     EQWEFGGKVND"
     gene            26677..27507
                     /gene="thiD"
                     /locus_tag="P6L01_000260"
     CDS             26677..27507
                     /gene="thiD"
                     /locus_tag="P6L01_000260"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDP3029361.1"
                     /translation="MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVA
                     QNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIP
                     YVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
                     AGRIFINEIGSKGIIIKGGHSNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFS
                     AVITAELAKGRPLFEAVHKAKKFISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
                     "
     gene            27491..28282
                     /gene="thiM"
                     /locus_tag="P6L01_000261"
     CDS             27491..28282
                     /gene="thiM"
                     /locus_tag="P6L01_000261"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDP3029362.1"
                     /translation="MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPE
                     EAEEFYKVAQALLINIGTLTAQNEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFC
                     KLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVAIAKKAYAIYKTAIVIT
                     GKEDVIVQDNKAIVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVF
                     NIAAEVAAENENCGGPGTFSPLLLDTLYHLNETTYQQRIRIQEVE"
     gene            28284..28925
                     /locus_tag="P6L01_000262"
     CDS             28284..28925
                     /locus_tag="P6L01_000262"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDP3029363.1"
                     /translation="MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREK
                     GESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIA
                     QYFTDKIIGLSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHIPVGPEMIATFKEM
                     NPQLPIVAIGGINTNNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN"
     gene            29011..29883
                     /gene="yidC"
                     /locus_tag="P6L01_000263"
     CDS             29011..29883
                     /gene="yidC"
                     /locus_tag="P6L01_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDP3029364.1"
                     /translation="MKKKALLPLFLGIMVFLAGCDYSKPEKRSGFFYNTFVDPMKNVL
                     DWLGNNLLNDNYGLAIIILVLVIRIILLPFMLSNYKNSHMMRQKMKVAKPEVEKIQEK
                     VKRARTQEEKMAANQELMQVYKKYDMNPIKSMLGCLPMLIQLPIIMGLYFVLKDQLVD
                     GLFKYPHFLWFDLGRPDIWITIIAGVLYFIQAYVSSKTMPDEQRQMGYMMMVISPIMI
                     IWISLSSASALGLYWSVSAAFLVVQTHFANIYYEKVAKKEVQPFIEAYEREHNGGSNK
                     KGKNTQVVSKKKKK"
     gene            complement(30796..31443)
                     /locus_tag="P6L01_000264"
     CDS             complement(30796..31443)
                     /locus_tag="P6L01_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDP3029365.1"
                     /translation="MQQSDVISAAKKYMESIHQNDYTGHDIAHVYRVTALAKSIAENE
                     GVNDTLVIELACLLHDTVDEKVVDANKQYVELKSFLSSLSLSTEDQEHILFIINNMSY
                     RNGKNDHVTLSLEGQIVRDADRLDAIGAIGVARTFQFAGHFGEPMWTEHMSLDKINDD
                     LVEQLPPSAIKHFFEKLLKLESLMHTDTAKMIAKERHDFMMMYLKQFFTEWNYHD"
     gene            complement(31471..>32708)
                     /gene="cls"
                     /locus_tag="P6L01_000265"
     CDS             complement(31471..>32708)
                     /gene="cls"
                     /locus_tag="P6L01_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDP3029366.1"
                     /translation="KGLELIVDEQLAALKNENFSNSNYQIVKFKEMIQMLLYNNAAFL
                     TTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQYYIIQNDELGRTILNELGKKAEQGV
                     EVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFFPSKLPLINLRMNNRNHRKIVVIDGQ
                     IGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGDAVNALQLRFILDWNSQATRDHISYD
                     DRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGYLKMISSAKKSIYIQSPYFIPDQAFL
                     DSIKIAALGGVDVNIMIPNKPDHPFVFWATLKNAASLLDAGVKVFHYDNGFLHSKTLV
                     IDDEIASVGTANMDHRSFTLNFEVNAFIYDQQIAKKLKQAFIDDLAVSSELTKARYAK
                     RSLWIKFKEGISQLLSPIL"
BASE COUNT        11588 a         4978 c         6307 g         9835 t
ORIGIN      
        1 aatgaaagcg taaaaatggt aaaatatacc aaaattgatt gtgatataat tataaggaaa
       61 atgagcaatt tatgaaaaaa gtttacgtac aaatcgtaga attaaaacta aataattatc
      121 aaaacaacgt caatatttag ttgaataatc agactttaga ccatggtcaa gtggggaaga
      181 acagcatata ttagtaaagg tgaatgattt gttattaatc aatcgaaaat agaaagacaa
      241 gattttaacg attaaaataa actattttac aaataaagta aaattaattt ataatgctaa
      301 taatgcaaaa aattaaaaag taatggacaa aggagataaa tgatatggct caagaggtaa
      361 taaaaataag aggtggacgc acactaaatg gggaagttaa tatcagtggc gcgaaaaaca
      421 gtgctgtagc tatcattcct gcaacattat tagctcaagg acatgtgaaa ttagaagggt
      481 taccgcaaat ctctgatgtt aaaactttag tgagtttatt agaagattta aatattaagg
      541 catcactaaa tggtacggaa ttagaagttg atacaactga aatacaaaat gctgcattac
      601 caaataataa agttgaatca ttacgtgctt catactatat gatgggtgcc atgttaggta
      661 gatttaaaaa atgtgtgatt ggtttaccgg gaggatgtcc acttgggcca cgtccaattg
      721 atcaacatat taaaggattt aaagctttag gtgctgaaat tgatgaatca agcacgacat
      781 caatgaaaat tgaagctaaa gaattaaaag gtgcgcatat ttttctagat atggttagcg
      841 taggtgcaac aattaacatt atgctagctg cagtttatgc aacaggacaa actgtaattg
      901 aaaatgctgc taaagaaccg gaagtagttg atgttgctaa cttcttaaca agtatgggag
      961 ctaatattaa gggagcgggc acatcaacaa ttaaaatcaa tggtgtcaaa gaattacatg
     1021 gttctgagta tcaagttata cctgatagaa ttgaagcagg cacatatatg tgtatcgctg
     1081 cagcatgtgg agaaaatgtt atattaaata atattgttcc gaaacatgta gaaacattaa
     1141 ctgctaaatt tagtgaatta ggtgtgaatg ttgatgtaag agacgaaaga attcgcatca
     1201 ataataatgc gccatatcaa tttgttgata ttaaaacact agtatatcca ggttttgcta
     1261 ctgatttgca acagcctatt acaccattat tatttatggc aaatggtcct tcatttgtaa
     1321 ctgatacgat ttatccggag cgttttaaac atgttgaaga attaaagcgc atgggtgcaa
     1381 atattgaagt tgacgaaggc acagcaacga taaaaccatc aacattacat ggtgctgaag
     1441 tttatgctag cgatttaaga gcaggagctt gtttaattat tgctggttta attgctgagg
     1501 gtgtaactac aatttacaat gtaaaacata tttatagagg ttatacagat attgtagaac
     1561 acttaaaagc tttaggtgca gatatttgga cggaaactgt atagatttat cttgttttgt
     1621 ggtataataa ttatactgat taaaaaaggg tgttcgttta cattctaaat ttattgatga
     1681 gaggtgaaga tgatggaagt atgtccgtat ctcgaagaaa cttttaaaat acttggtaga
     1741 agttggaatg gattaatcat taattatctc tcaagatgta atgactgttc agcacacttt
     1801 tccgatatga aaagagattt gaaaacaata acaccacgtg ctttaagtct taagttgtca
     1861 gagcttgcac aatgggagtt agttgagaag caaatcattt ctacgagtcc agtacaaatt
     1921 atttatgtgc taactgaaaa aggtaaagcg ttagcagagg ctttacatcc aattgaagca
     1981 tgggcgcaat catatgtcga tttaacagat caacgtactg ctaaataatt acatgacgtt
     2041 taaacatagc attgattata actatttcta agtcttcgca ttatttgcga tgatgtggga
     2101 atagttattt ttatttaaaa atataaaaaa atagatgcag caaaatttta aagcatttta
     2161 ttttgaacat attaaaatgg agcgtatcat aatggaatgt aatgtttata tcgtatgcat
     2221 tacggataaa taatatataa atcattcttg aggagtgaaa gaataatgag agactacaca
     2281 aagcaataca ttaatggcga atgggtagaa agtaatagta atgaaacgat agaagttata
     2341 aatccagcaa ccgaagaagt aatcgggaaa gttgctaaag gtaataaagc tgatgttgat
     2401 aaagccgtcg aggcggcaga cgatgtttat ttagagttcc gtcatacatc tgtgaaagaa
     2461 agacaagcgt tattagataa aattgtaaaa gaatatgaaa acagaaaaga cgatattgta
     2521 caagctatta cggatgaatt aggtgctcct ttatcattat ctgagcgtgt ccattatcaa
     2581 atgggactaa accattttgt tgcagcgaga gacgcattag ataactacga atttgaagaa
     2641 cgccgcggag atgatttagt tgttaaagaa gcaatcggtg tatctggatt aattacaccg
     2701 tggaacttcc ctacaaacca aacatcatta aaattagcag cagcatttgc ggctggtagt
     2761 ccagttgtac ttaaaccatc tgaagaaaca ccatttgcag ctgttatttt agctgagatt
     2821 tttgataaag tcggtgttcc taaaggtgta tttaaccttg ttaatggtga tggtgctggt
     2881 gttgggaatc ctttatctga acatcctaaa gtacgcatga tgtcattcac aggatcaggc
     2941 cctactggtt ctaaaattat ggaaaaagcc gctaaagatt ttaaaaaggt atcattagag
     3001 cttggtggca aatcaccata tatcgtccta gatgacgtag atattaaaga agcggctaaa
     3061 gcaacaacag gcaaagttgt taataatact ggtcaagtat gtacagctgg tacacgtgtt
     3121 ttagtgccta acaaaattaa agatgcattc ttagctgaat taaaagaaca atttagccaa
     3181 gtgcgtgtcg gtaatccaag agaagatggt acacaagtag gccctatcat tagtaaaaaa
     3241 caatttgatc aagtacaaaa ttatattaat aaaggtattg aagaaggtgc tgaattattt
     3301 tatggtggtc ctggtaaacc agaaggactt gaaaaaggat actttgcacg tccgacaatt
     3361 tttattaatg tagataatca aatgacgata gcacaagaag aaatttttgg gccagtaatg
     3421 tcagttatca cttataacga tttagatgaa gcgattcaaa ttgcaaatga tacaaaatat
     3481 ggtttggcag gatatgttat tggtaaggac aaagaaacat tgcataaagt agctcgttct
     3541 attgaagcag gtacagtaga aataaacgaa gcaggtagaa agccagattt accatttggt
     3601 ggctataaac aatctggttt aggtcgtgaa tggggcgatt atggtattga agagttctta
     3661 gaagtgaaat ctatagctgg atattttaaa taaatttgat ttttgaatta aaaatcgcaa
     3721 taaaacagtg cacatgacta attaagtttt gtgtactgtt ttaattttgc aatttttata
     3781 aatagatttt gtaattaaaa taaaaatttg ctatagttat tcatgtattt aaaaggttgg
     3841 ggattagcat aatgggattg tgctagcaca gttatttatg cattgtcatg cctatctatt
     3901 acttactaac taaaaaataa tgaaatgggt gtaaactata tgcctgaaag agaacgtaca
     3961 tctcctcagt atgaatcatt ccacgaattg tacaagaact atactaccaa ggaactcact
     4021 caaaaagcta aaactcttaa gttgacgaac tatagtaaat taaataaaaa agaacttgtt
     4081 ctagctatta tggaagcaca aatggaaaaa gatggtaact attatatgga aggtatctta
     4141 gatgatatac aaccagatgg ttatggtttt ttaagaacag tgaactattc taaaggggaa
     4201 aaagatattt atatatctgc tagccaaatt cgtcgttttg aaattaaacg tggggataaa
     4261 gtaactggga aagttagaaa acctaaagat aacgaaaaat attatggctt attacaagtt
     4321 gactttgtca atgaccataa cgcagaagaa gtgaagaaac gtccgcattt ccaagctttg
     4381 acaccacttt atccagatga gcgtattaaa ttagagacag aaacacaaaa ttattcaacg
     4441 cgcatcatgg atttagtaac accgattggt ttaggtcaac gtggtttaat agtggcgcca
     4501 cctaaagcag gtaaaacatc gttattaaaa gaaatagcga atgcaatcag tacgaacaaa
     4561 ccagatgcaa agctatttat tttgttagtt ggcgagcgtc ctgaagaggt gacagattta
     4621 gaacgctcag tagaagctgc tgaagtcgtt cattcaacgt ttgacgaacc accagaacac
     4681 catgttaaag tagctgaatt attacttgaa cgtgcaaagc gtttagtaga aattggggaa
     4741 gatgtcatta ttttaatgga ttctataacg agattagcac gcgcttataa cttagttatt
     4801 ccaccaagtg gtcgtacatt atcaggtggt ttagatcctg catctttaca caaaccaaaa
     4861 gcattcttcg gtgcagcgag aaatattgaa gcgggtggaa gtttaacaat acttgcaact
     4921 gcattagttg atacgggttc acgtatggac gatatgattt acgaagaatt taaaggaaca
     4981 ggtaacatgg agttacattt agatcgtaaa ttgtctgaac gtcgtatctt ccctgcaatt
     5041 gatattggca gaagttcaac gcgtaaagaa gaattgttaa taagtaaatc tgaattagac
     5101 acattatggc aattaagaaa tctattcact gactcaactg actttactga aagatttatt
     5161 cgcaaactta aaaggtctaa gaataatgaa gatttcttca agcagctaca aaagtctgca
     5221 gaagaaagta ctaaaacggg tcgacctata atttaataaa cattatatag gggcttgcgt
     5281 tttgaattaa ttacctttat aattacacag tattgggtaa aaactcacaa ataactctgt
     5341 tccagatggt tcagggcaaa ggagctgaaa ataatgaaac aaggaattca tcctgagtat
     5401 caccaagtaa tctttttaga tactactaca aacttcaaat tcttaagcgg ttctacaaaa
     5461 acatcttcag aaatgatgga gtgggaagat ggaaaagaat acccagttat tcgtttagat
     5521 atttcatctg attcacatcc attctacact ggacgtcaaa aattcgctgc tgcggatgga
     5581 cgtgtggaac gtttcaacaa aaagtttggt cttaaatcaa acaactaata tagtaatcgt
     5641 taagctgtct gcatcctata atggaattgt agacagcttt tttatttata agcgtagtaa
     5701 gggtatatac ttacgactgt gttcaaagaa gtgactcaat accatgttgt taatacataa
     5761 tgataaattt ccccctttat taaagtttgt attgagtaga aacaccgcac gaataagaag
     5821 caacaaatta gaatagctgt atataaatat gcaagacaaa tttataaaat aattgttcat
     5881 gtacaatatc gatataatta tgaaaaagac atttatgaat atttgaaata tgatataata
     5941 aagcgattat gttttgaaaa aggtggaact ctcaatgtat gaaacttacc attccggttg
     6001 gatagaatgt attacaggta gtatgtttag cggtaaatcc gaagaactaa taagacgtct
     6061 aagaaggggt atatacgcta aacaaaaagt cgttgtattt aaaccggcga ttgatgatcg
     6121 atatcataaa gaaaaagtgg tctctcataa tggtaatgcc attgaagcga ttaatatttc
     6181 taaagcaagt gaaattatga cgcacaactt aactaatgtt gatgttatag gaatagacga
     6241 agtgcaattt tttgacgatg aaattgtaag tatagtagaa aaactatcag cagatggaca
     6301 ccgtgttatt gttgctggat tagatatgga ttttaggggc gaaccgttcg aaccaatgcc
     6361 taaattgatg gctgtcagtg aacaagtaac gaaacttcaa gcagtttgtg ctgtttgtgg
     6421 ttcttcatct agtagaacgc aacgattaat aaatggtaaa ccagctaaaa tcgatgatcc
     6481 gattatctta gtaggtgcca atgaaagtta tgagccacgt tgtcgcgcgc accatatcgt
     6541 agcacctagt gataataata aggaggaatt ataagtgttt gatcaattag atattgtaga
     6601 agaaagatac gaacagttaa atgaactgtt aagtgaccca gatgttgtaa atgattcaga
     6661 taaattacgt aaatattcta aagagcaagc tgatttacaa aaaactgtag atgtttatcg
     6721 taactataaa gctaaaaaag aagaattagc tgatattgaa gaaatgttaa gtgagactga
     6781 tgataaagaa gaagtagaaa tgttaaaaga ggagagtaat ggtattaaag ctgaacttcc
     6841 aaatcttgaa gaagagctta aaatattatt gattcctaaa gatcctaatg atgacaaaga
     6901 cgttattgta gaaataagag cagcagcagg tggtgatgag gctgcgattt ttgctggtga
     6961 tttaatgcgt atgtattcaa agtatgctga atcacaagga ttcaaaactg aaatagtaga
     7021 agcgtctgaa agtgaccatg gtggttacaa agaaattagt ttctcagttt ctggtaatgg
     7081 cgcgtatagt aaattgaaat ttgaaaatgg tgcgcaccgc gttcaacgtg tgcctgaaac
     7141 agaatcaggt ggacgtattc atacttcaac agctacagtg gcagttttac cagaagttga
     7201 agatgtagaa attgaaatta gaaatgaaga tttaaaaatc gacacgtatc gttcaagtgg
     7261 tgcaggtggt cagcacgtaa acacaactga ctctgcagta cgtattaccc atttaccaac
     7321 tggtgtcatt gcaacatctt ctgagaagtc tcaaattcaa aaccgtgaaa aagcaatgaa
     7381 agtgttaaaa gcacgtttat acgatatgaa agttcaagaa gaacaacaaa agtatgcgtc
     7441 acaacgtaaa tcagcagtcg gtactggtga tcgttcagaa cgtattcgaa cttataatta
     7501 tccacaaagc cgtgtaacag accatcgtat aggtctaacg cttcaaaaat tagggcaaat
     7561 tatggaaggc catttagaag aaattataga tgcactgact ttatcagagc agacagataa
     7621 attgaaagaa cttaataatg gtgaattata aagaaaagtt agatgaagca attcatttaa
     7681 cacaacaaaa agggtttgaa caaacacgag ctgaatggtt aatgttagat gtatttcaat
     7741 ggacgcgtac ggactttgta gttcacatgc atgatgatat gccgaaagcg atgattatga
     7801 agttcgactt agcattacaa cgtatgttat taggagagcc tatacagtat atagttggct
     7861 ttgcctcatt ttatggtaga acgtttgatg taaactcaaa ttgtttgata ccaagacctg
     7921 aaactgaaga agtaatgttg catttcttac aacagttaga agatgatgca acaatcgtag
     7981 atatcggaac gggtagtggt gtacttgcaa ttactttgaa atgtgaaaag ccggatttaa
     8041 atgttattgc tactgatatt tcacttgaag caatgaatat ggctcgtaat aatgctgaga
     8101 agcatcaatc acaaatacaa tttttaacag gggatgcatt aaagccctta attaaagaag
     8161 gtatcaagtt gaacggcttg atatctaatc caccatatat agatgaaaaa gatatggtta
     8221 cgatgtctcc aacggttacg agattcgaac cacatcaggc attgtttgca gataaccatg
     8281 gatatgctat ttatgaatca atcattggag atttacctca cgttatggaa aaaggcagcc
     8341 cagttgtttt tgaaattggt tacaatcaag gtgaggcact taaatcaata attttaaata
     8401 aatttcctga caaaaaaatc gacattatta aagatataaa tggccacgat cgaatcgtct
     8461 catttaaatg gtaattagaa gttatgcctt tgctatgatt agttaagtgc atagcttttt
     8521 gctttatatt atgataaata agaaaggcgt gattaagttg gatactaaaa tttgggatgt
     8581 tagagaatat aatgaagatt tacagcaata tcctaaaatt aatgaaataa aagacattgt
     8641 tttaaacggt ggtttaatag gtttaccaac tgaaacagtt tatggacttg cagcaaatgc
     8701 gacagatgaa gaagctgtag ctaaaatata tgaagctaaa ggccgtccat ctgacaatcc
     8761 gcttattgtt catatacaca gtaaagttca attaaaagat tttacatata ctttggatcc
     8821 acgcgtagaa aagttaatgc aggcattctg gccgggccct atttcgttta tattgccgct
     8881 aaagccaggc tatctatgtc gaaaagtttc tggaggttta tcatcagttg ctgttagaat
     8941 gccaagccat tctgtaggta gacaattatt acaaatcata aatgaacctc tagctgctcc
     9001 aagtgctaat ttaagtggta gaccttcacc aacaactttc aatcatgtat atcaagattt
     9061 gaatgcccgt atagatggta ttgttcaagc tgaacaaagt gaagaaggat tagaaagtac
     9121 ggttttagat tgcacatctt ttccttataa aattgcaaga cctggttcta taacagcagc
     9181 aatgattaca gaaatacttc cgaatagtat cgcccatgct gattataatg atactgaaca
     9241 gccaattgca ccaggtatga agtataagca ttactcaccc aatacaccac ttacaattat
     9301 tacagatatt gagagcaaaa ttggaaatga cggtaaagat tggtcttcta tagcttttat
     9361 tgtgccgagt aataaggtgg cgtttatacc aagtgaagcg caattcattc aattatgtca
     9421 ggatgacaat gatgttaaac aagcaagtca taatctttat gatgtgttac attcacttga
     9481 tgaaaatgaa aatatttcag cggcgtatat atacggcttt gagctgaatg ataatacaga
     9541 agcaattatg aatcgcatgt taaaagctgc aggtaatcac attattaaag gatgtgaact
     9601 atgaagattt tattcgtttg tacaggtaac acatgtcgta gcccattagc ggaaagtatt
     9661 gcaaaagagg ttatgccaaa tcatcaattt gaatcaagag gtatattcgc tgtgaacaat
     9721 caaggtgttt cgaattatgt tgaagactta gttgaagaac atcatttagc tgaaacgacc
     9781 ttatcgcaac aatttactga agcagatttg aaagcagata ttattttgac gatgtcgtat
     9841 tcgcacaaag aattaataga ggcacacttt ggtttgcaaa atcatgtttt cacattgcat
     9901 gaatatgtaa aagaagcagg agaagttata gatccatacg gtggaacaaa agaaatgtat
     9961 gtacatacct atgaagaact tgtaagttta attttaaaat taaaagatat tatttgctag
    10021 taggaattct gtataattag tatatgttaa cagttaaata agttataata gaggagtgta
    10081 gcactttgct atattccttt tttattttta ggaagggtgg gcttttatga aagatttgac
    10141 aatgttatta gacgaattaa aagatatgtc cttttttaat aaaggagata tatgtttaat
    10201 tggatgttca acatcggaag tcattggcga aaaaataggg actgtcggtt cgatggaagt
    10261 tgcagaaact atttttaatg cattagatgt tgtgagtaaa gagacaggcg ttacttttgc
    10321 ttttcaagga tgtgaacata tcaacagagc gataacaata gaaaaatctc agtataatcc
    10381 attaacaatg gaagaagttt ccgtcgttcc agatgtacat gctggaggga gtttggcaac
    10441 atacgctttt cagcatatga aggatccgat cgtggtcgaa catattactg ttccttgtgg
    10501 aattgatatt ggtcaaacat taataggcat gcatattaaa catgtatgtg tacctgtaag
    10561 gacctcagta aagcaagtag gacaggcaat tgtaactatt gccacatcta ggccgaaaaa
    10621 gattggtggc gaacgtgcta aatacaaata agtttttttg aaagaagggg agattttatg
    10681 tcttatatca ccaagcaaga taaagttatc gcagaagcaa tcgagagaga atttcagaga
    10741 caaaatagca acatagagtt aatcgcatcg gaaaattttg tatcggaagc ggttatggaa
    10801 gcacaaggtt cagtgttgac taataagtat gctgaaggct atccaggacg ccgatattat
    10861 ggtggatgtg agtttgtaga tgttactgaa agcatcgcaa ttgatcgtgc taaagcattg
    10921 tttggagctg aacatgtcaa tgttcaacca cattcaggtt cacaagcgaa catggctgtt
    10981 tacttagttg cattagaaat gggcgacaca gttttaggta tgaatttgag tcatggtggt
    11041 cacttgacac atggagcgcc tgttaatttt agtggtaaat tctacaattt cgttgaatat
    11101 ggagtagata aagacacaga acgaatcaat tatgatgaag ttcgtaaatt agcgttagag
    11161 cataagccta agcttattgt ggcaggagca tcagcatatt caagaacaat tgacttcaaa
    11221 aagtttaaag aaatcgcaga tgaagtaaac gctaagttaa tggtagacat ggcacatatt
    11281 gcaggattag tagcggcagg tttacatcca aatccagtag aatatgctga ttttgtaaca
    11341 actacaacac acaaaacatt acgcggacca cgtggtggta tgattttatg taaggaagaa
    11401 tataaaaaag acatagataa aacaattttc cctggtattc aaggtggacc tcttgagcat
    11461 gttattgcag caaaagcagt tgcttttgga gaagcgttag aaaataattt caaaacgtat
    11521 caacaacaag tggttaaaaa cgcaaaagtt cttgcagaag cattaattaa tgaaggattt
    11581 agaattgttt ctggcggtac agataatcac ttagtagctg ttgatgtaaa agggtctata
    11641 ggacttactg gtaaagaagc tgaagagact ttagattcag ttggtatcac atgtaacaaa
    11701 aataccattc cgttcgatca agaaaaacct tttgtaacga gtggtatacg tttaggtaca
    11761 cctgctgcaa caacgcgtgg atttgatgaa aaagcttttg aggaagttgc aaaaatcatc
    11821 agtttagcat tgaaaaatag taaagatgaa gaaaaattac aacaagctaa agaacgcgtt
    11881 gcgaaattaa cagctgaata tcctctatat caataaacat acacaaagat aggagtaatt
    11941 tttatgagta aagtacacgt tttcgatcat ccattaattc aacacaaact aagctatatt
    12001 agagatgtaa atacaggtac taaagaattt agagaacttg tagacgaagt tggtatgttg
    12061 atggcatatg aagtaactag agacttagag cttcaggatg ttgatattga aacgcctgta
    12121 actaaaatga cggctaaacg attagctggt aaaaaattag ctatcgttcc aattttaaga
    12181 gctggtttag gtatgactga tggtatttta agtttagtac ctgcagccag agttggacat
    12241 atcggtttat atcgagatcc cgaaacatta aaggctgtag agtattttgc taagttacct
    12301 caagatatta ctgaaagaca aatcattgtt gttgatccta tgttagcaac aggcgcatca
    12361 gcaattgaag ctattacttc attgaagaaa cgtggcgcta aaaatattcg cttcatgtgt
    12421 ttaattgcag caccagaagg cgtagaaaag atgcatgaag cacatccgga tgtagatatt
    12481 tatattgctg cacttgatga aaagctaaat gataaagcat atatcacacc agggttaggc
    12541 gacgctggag atagattgtt tggtactaaa tagaagaatc ggagataagt agtttgatga
    12601 aaaagattat gaccatattt ggaacaagac ccgaagccat aaagatggct cctttagtta
    12661 aagctttgga acaagaaaaa atgctcgagc caattgttgt tgttacagca cagcatagag
    12721 aaatgcttga ttcagtatta agtacctttg aaatcaaacc aaaatacgat ttgaatatta
    12781 tgaaatcagg acagacactt tctgagataa cttctaaatc aatcactcaa ttagaacaag
    12841 tgattcaatt agaaaaacca gacatggtac ttgtacatgg tgatacgatg acgacatttg
    12901 caggtggttt agcggcattt tataaccaag tgccgattgg acacgttgaa gcagggctta
    12961 gaagttatga taaatattca ccttttccag aagaggtgaa tagacaatta gttggtgtat
    13021 tagctgactt acattttgca cctactaaaa atgctgcatc gcatttgtta agtgaaggga
    13081 aatattcaga aagtgttgtt gtcactggta atactgcgat tgatgctatg aaatatacag
    13141 ttgatgacaa ttataaatct aacatcatgg ataaatacca tgacaaaaaa ttcattctta
    13201 tgacagcaca ccgacgagaa aatatcggga aacccatgga aaatatattt aaagcggtta
    13261 gacgcttaat agatgagtat actgatttag cactggtata tccaatgcat aaaaacccga
    13321 aagttcgaga ggtggctcaa aagattttag gtagtcatga cagaattgaa ttgattgaac
    13381 ctttggacgt agtagatttc cataattttg ctaaaaaatc ttattttatt ttgacagatt
    13441 caggtggaat tcaggaagaa gcgccatcat ttaataagcc agttttagtt ttgcgaagcg
    13501 ttacagaacg tcctgaaggt gttgaagctg gcacattaaa agtcattgga acaaataagc
    13561 aaaatgtgta tcaagctgca aaagaattaa tcgatgatga gagattatat catcaaatgt
    13621 ctgaagcatc taatccttac ggtgatggat ttgcttcaga aagaatagtt aatcatatta
    13681 agtattattt gaatttaatc actgaaaaac caagcgattt ctgaggaaaa tagacttttt
    13741 gtctaattgt gtctaaattg tgtctaacta tgcttatttt tttacaacaa tttgaataga
    13801 attgacacca caaatcgcct atattatcat ttagtttgta tgagtggaac ggttttcatg
    13861 attgatatca gtcacacgga ggcatataaa atgagtcgtt tctacaacat ttttaaacag
    13921 ttcattcaat attattttta tctaataata atattgggag gattatacct ttatacacac
    13981 catgcattta tcttaggatt aatcattggt gttactggtt ctgcaatcaa cacatgtatt
    14041 tttgaatgct atttagctaa agctaaaaga ccagacacta tgcatatttc aactggaaat
    14101 atgtggcgat acttagttgc aattattgcc tgtatgattt ggtaccttaa taaagcgcat
    14161 gtaagtatca tcggtataat tattggttta atgatttcat atgttgtagt tatcatacgt
    14221 cctttactaa aggtgagcaa ataaattaag aaagaggtga gattatggat cacaaatccc
    14281 cgctcgtgag ttggaattta ttcggttttg atatcgtttt caatttatca agtatattga
    14341 tgatacttgt tacggcgttt cttgtttttc tacttgctat catttgtacg cgtaatttga
    14401 aaaaaagacc aactggcaaa caaaatttcg ttgaatggat ttttgatttc gtgaggggaa
    14461 tcattgaagg taacatggct tggaaaaaag gtggtcaatt ccacttctta gcagtaacgc
    14521 tgattctgta catttttata gctaatatgt taggtcttcc gttttctata gtaacgaaag
    14581 atcacacatt gtggtggaaa tcaccgacag ctgatgcaac agtgacttta acgttgtcta
    14641 caacgataat actgttaact cacttttatg gaattaaaat gcgtggtacg aaacaatatc
    14701 ttaaaggtta tgtacagccg ttttggccat tggcaattat taatgttttt gaagagttca
    14761 cttcaacatt aacgcttggt ctgcgtttgt acggtaacat atttgcaggt gagatactat
    14821 taacattact tgctggctta ttctttaacg aaccagcatg gggttggatt attagtatcc
    14881 caggattaat tgtttggcaa gcattttcaa tatttgtagg aacaatccaa gcatatatct
    14941 ttattatgct ttcgatggtt tatatgtcac ataaagtggc agatgaacac taaaaatttc
    15001 aataattata tacaatcaca ggaggaaatt aaattatgaa tttaatcgca gcagcaatcg
    15061 caattggttt atcagcatta ggagcaggta tcggtaacgg tttaatcgtt tcaagaacag
    15121 ttgaaggtgt agcacgtcaa ccagaagcac gtggtcaatt aatgggtatc atgttcattg
    15181 gtgtaggttt agttgaggca ttacctatca tcggtgtagt aattgcattc atgacatttg
    15241 ctggataatt aacagataaa agaggtcggg acaaagcgca taggacataa ttcatgatgc
    15301 atatatagta atatctttga actttattaa atagttgaga tatgaatgca ccatgcctat
    15361 cgcataaatt cagtaggtcc taacctcgtc gtttttttct atataacact agcgattatt
    15421 ttaatgaaag gagtgtcatg aacccgtgac tgaaacagct aacttattcg ttcttggtgc
    15481 agctggaggc gttgagtggg gtactgtgat tgtacaggtc ctaactttca tcgtgttact
    15541 tgcgttactt aaaaagttcg catggggtcc attgaaagat gtaatggata aacgtgaaag
    15601 agatattaac agagatatcg atgacgcaga acaagctaag ttaaatgcac agaaacttga
    15661 agaagaaaat aaacaaaaac ttaaagaaac acaagaagaa gttcaaaaga ttttagaaga
    15721 tgctaaggtt caagcacgtc aacagcaaga acaaattatt catgaagcaa acgtacgtgc
    15781 aaacggtatg attgaaacag cacaaagtga aatcaatagc caaaaagaac gtgccattgc
    15841 agatattaat aatcaagtat ctgaactatc agtgttaatt gcttctaaag ttcttagaaa
    15901 agaaatttct gaacaagacc caaaagcatt ggttgacaag tatctaaaag aggcaggcga
    15961 taaataatgg taaaagtagc taacaagtat gctaaagcat tatttgacgt gtcattagat
    16021 acaaataatt tagagactat taatgaagaa ttaacagtta taaatgaagc agtaaaagat
    16081 aaaattgagc aattgaaaat ggttgacagt aatccaactc aaacagcaga acaacgtcgt
    16141 gagttgatta atggtgtatt tactgacatt aatccataca ttaaaaatat gatgtacgtg
    16201 ttagcagata atagacatat ctcattaata gctgacgtat tcaaggcgtt ccaaagctta
    16261 tataacggac actacaatca agattttgca acaattgagt caacatatga attgagtcaa
    16321 gaagagttag ataagattgt caaactagta actcaacaaa cgaagttatc taaagttatt
    16381 gtagatacaa aaattaatcc agatttaatt ggtggattta gagttaaagt cggcacaact
    16441 gtattagatg gtagtgttag aaatgatctt gtccaattac aaagaaaatt tagaagagtt
    16501 aattaattat aaagaggagt gacatagatg gccataaaag ctgaagaaat cagtgcatta
    16561 cttcgctcac aaattgaaaa ttatgagtca gaaatgtccg taactgatgt aggtactgta
    16621 ttacaaattg gtgatggtat tgcattaatt cacggattaa atgacgttat ggctggtgag
    16681 ctagtagaat tccataacgg cgtacttggt ttagcccaaa accttgaaga gtcaaacgtg
    16741 ggtgtggtta ttttaggacc atatacaggt attactgaag gtgacgaagt taaacgtact
    16801 ggtcgtatca tggaagtacc agtaggtgaa gaactaatcg gaagagttgt taatccatta
    16861 ggacaaccta ttgatggaca aggaccgatt aacacaacta aaacacgtcc agtagagaaa
    16921 aaagctactg gtgtaatgga tcgtaaatca gtagatgagc cattacaaac aggtatcaaa
    16981 gcaattgatg ctttagtacc aattggtaga ggtcaacgtg agttaatcat tggtgaccgt
    17041 caaacaggta aaacaacaat tgcaattgac acaattttga accaaaaaga tcaaggtacg
    17101 atttgtatct atgttgctat tggtcaaaaa gattcaacag taagagcaaa tgttgaaaag
    17161 ttaagacaag caggcgcttt agactacact attgttgtag cagcatcagc ttctgaacct
    17221 tctccattat tatatattgc accatattca ggtgtaacaa tgggtgaaga attcatgttt
    17281 aacggtaaac atgttttaat cgtttatgat gatttaacta aacaagcagc agcttatcgt
    17341 gagttatcat tattattacg tagacctcca ggtcgtgaag catacccagg tgacgtattc
    17401 tacttacata gtagattatt agaaagagca gcaaaattaa acgatgactt aggtggcggt
    17461 tcaattactg cattaccaat tatcgaaaca caagctggtg atatttcagc ttatgtacca
    17521 acaaacgtta tttcaattac agatggacaa atcttcttac aatctgattt attcttctca
    17581 ggtgtaagac cagcgattaa tgccggacaa tctgtatctc gtgttggtgg atctgcacaa
    17641 attaaagcaa tgaagaaagt tgctggtacg ttacgtcttg acttagcgtc atacagagaa
    17701 cttgaatcat ttgcacaatt cggttcagac cttgatgaat ttactgcaag taaattagaa
    17761 cgtggtaaac gtaccgttga agtcttaaaa caagatcaaa acaaaccatt accagtcgaa
    17821 caccaagtgt tgattattta tgcattaaca aaaggatatt tagatgatat tccagttgta
    17881 gatatcacac gttttgaaga cgagttaaac cactgggcag aatcaaatgc tactgaactg
    17941 ttaaatgaaa tcagagaaac tggtggctta ccagatgctg agaagtttga cacagcaatt
    18001 aacgaattca aaaaaagctt tagcaaatct gaataataaa caagtttagt ataaggtggt
    18061 gagatagtgg cttctcttaa agaaatagat actcgaataa aatcaaccaa aaaaatgaag
    18121 cagattacga aagcgatgaa catggtatca agttcaaaac ttcgtagagc tgaaaaaaat
    18181 acaaaacaat tcacaccata tatggataaa atgcaagatg caattactgc agttgcaggg
    18241 gcaagtagca atacaaacca tccaatgcta agacctcgta aaattactag aagtggatat
    18301 ttagttatca cgagtgataa aggtttagca ggtgcatata gtgcaaacgt gcttaaaaaa
    18361 ttgattactg atattgaagc gaaacatcaa gatagtagcg aatacagtat tgtagtttta
    18421 gggcaacaag gtgttgattt cctaaaaaat agaggttatg acattgagta ttctcaagta
    18481 gacgtacctg atcaaccttc tttcaaatct gttcaagcac tagctaacca tgctatagac
    18541 ttatacagtg aagaagaaat tgatgaatta aatatatact atagtcatta tgtcagcgtt
    18601 cttgaaaaca agcctacatc tagacaagta ttaccattat ctcaagagga ttctagtaag
    18661 gggcatggtc atttgtcttc ttatgaattt gagccagata aagaatctat cttaagtgta
    18721 atcttgcctc aatatgttga gagtttgatt tacggaacaa tattagacgc aaaagcaagt
    18781 gagcatgcaa cacgtatgac tgcgatgaaa aatgccactg ataatgcaac tgaacttatt
    18841 gatgacttat cattagaata taacagagcg agacaagcag aaattacgca acaaattact
    18901 gaaattgttg gtggttccgc agcgcttgaa taatatttaa aggaggaaaa tagcatggga
    18961 attggccgtg taactcaagt tatgggtcct gtaattgatg ttcgatttga acataacgaa
    19021 gttcctaaaa ttaataacgc cttggttatt gatgtgccta aagaagaagg tacaatacaa
    19081 ctaacattag aagttgcgct gcaattaggt gacgacgttg ttcgtacaat tgcgatggat
    19141 tcaactgatg gtgtccaaag aggcatggat gtaaaagata caggcaaaga aattagtgta
    19201 cctgttggtg acgaaacatt aggtcgtgta tttaatgtac taggtgaaac aattgacctt
    19261 aaagaagaaa ttagtgattc tgttcgccgc gatcctatcc atcgtcaagc accagcattc
    19321 gatgaacttt caacagaagt tcaaatttta gaaacaggta ttaaagtagt agatttacta
    19381 gcaccttata ttaaaggtgg taaaatcgga ttgttcggtg gtgccggtgt aggtaaaaca
    19441 gtattaatcc aagaattaat taacaacatc gctcaagagc acggtggtat ttctgtattc
    19501 gccggtgtag gtgaacgtac tcgtgaaggt aacgatttat acttcgaaat gagtgacagt
    19561 ggtgtaatta agaaaacagc catggtattc gggcaaatga atgagccacc tggtgcacgt
    19621 atgcgtgttg cattatctgg tttaacaatg gctgaatatt tccgtgacga acaaggtcaa
    19681 gacgtattat tattcatcga taacattttc agatttacac aagctggttc tgaggtatct
    19741 gcattattag gtcgtatgcc ttctgcagta ggttaccaac caacacttgc tactgaaatg
    19801 ggacaattac aagaacgtat tacgtctaca acaaaaggat cagttacttc tattcaagcg
    19861 gtattcgtac ctgccgatga ctatactgac ccagcgcctg cgacagcgtt tgcccattta
    19921 gatgcaacta ctaacttaga acgtaaatta actgaaatgg gtatttatcc agccgtggat
    19981 ccattagcgt ctacatcaag agcattggaa ccatcaattg taggtcaaga acattatgaa
    20041 gtagctcgtg atgtacaatc aacacttcaa aaataccgtg aattgcaaga tatcattgct
    20101 atcttaggta tggacgaatt atctgatgaa gataaacaaa cagttgaacg cgcacgtaga
    20161 attcaattct tcttatctca aaacttccac gtagcggaac aatttactgg tcaaaaaggt
    20221 tcttatgtac ctgttaagac aacagttgca aactttaaag atatcttaga tggtaaatat
    20281 gaccatattc cagaagatgc attccgttta gttggtagca tggatgatgt tattgcaaaa
    20341 gctaaagata tgggtgttga agtataacaa ttaggaggaa tggataatga atacattaaa
    20401 cctagatatt gtcactccta atggttctgt ttacaatcgt gataatgttg aactcgttgt
    20461 tatgcaaaca acagctggtg agataggtgt catgagtgga catattccaa ctgtagctgc
    20521 tttaaaaaca ggctttgtaa aagtgaaatt tcacgatgga actgaatata ttgctgtaag
    20581 cgatggcttt gttgaagtta gaaaagataa agtttcaatc attgttcaga ctgcagaaac
    20641 tgcaagagaa attgatgttg aaagagctaa attagccaaa gcaagagcag agtctcactt
    20701 ggaaaatgat gacgacaata ctgatattca tagagccgaa agagctttag agagagcaaa
    20761 taaccgtttg cgtgtggctg aattaaaata gtaaataaag ggtcgaagat gtgatttcat
    20821 atcttcgacc cttttttgaa ttatattgat ttaaagatac aaaacatgag aggggggaag
    20881 gaattgataa agaaccatta aagatttatg atgtagtggt tctttatcat taaacacagc
    20941 taatgtgtat ttaaaaatag gaatacatga gtaaaactca tgtataagaa atactaattt
    21001 ttaaagaaaa agtatttctt tatgttgggg ccccgtcaac tactgccaaa tacaacacta
    21061 tagagtctag acattgattt atgtccgact cccaagaata gttttacttt tttacaatca
    21121 ctaatagatt gctaaaatca aaatttcctt caccactatc tacagtcgac atttcatttt
    21181 ttgaaattat ctacattttt tcataccaag atattttata gttatgatat ttatgtaaaa
    21241 agaattatat agtaagttag cttaaacttt actaaaaacg ggtattaaac tttgtatcat
    21301 tatttaaatt tttcatgtac aatgtaatac agtaatctta tgaggtgata aaatggatta
    21361 tatcggacaa tatgcagtta tccatttagt gttacatgtt gtatgtattt gtattgccta
    21421 ttgggcttta caatcaatta gattagatca attttttaaa aaaggatacg ccactcaatt
    21481 acaagtgtgt atgatatttg ttgctatttt attaggcact gcagtaagca attttattgt
    21541 agatttgtta caatactcga cgcaggtaaa atatttaata aaataagtct aactctatga
    21601 tttgtaatca aaactagata taattaaata atgacttaaa ataattttaa aatagggaaa
    21661 tgtaaagtaa taggagttct aagtggagga tttacgatgg ataaaatagt aatcaaaggt
    21721 ggaaataaat taacgggtga agttaaagta gaaggtgcta aaaatgcagt attaccaata
    21781 ttgacagcat ctttattagc ttctgataaa ccgagtaaat tagttaatgt tccagcttta
    21841 agtgatgtag aaacaataaa taatgtatta acaactttaa atgctgacgt tacatacaaa
    21901 aaggacgaaa atgctgttgt cgttgatgca acaaagactc taaatgaaga ggcaccatat
    21961 gaatatgtta gtaaaatgcg tgcaagtatt ttagttatgg gacctctttt agcaagacta
    22021 ggacatgcta ttgttgcatt gcctggtggt tgtgcaattg gaagtagacc gattgagcaa
    22081 cacattaaag gttttgaagc tttaggcgca gaaattcatc ttgaaaatgg taatatttat
    22141 gctaatgcta aagatggatt aaaaggtaca tcaattcatt tagattttcc aagtgtagga
    22201 gcaacacaaa atattattat ggcagcatca ttagctaagg gtaagacttt aattgaaaat
    22261 gcagctaaag aacctgaaat tgtcgattta gcaaactaca ttaatgaaat gggtggtaga
    22321 attactggtg ctggtacaga cacaattaca atcaatggtg tagaatcatt acatggtgta
    22381 gaacatgcta tcattccaga tagaattgaa gcaggcacat tactaatcgc tggtgctata
    22441 acgcgtggtg atatttttgt acgtggtgca atcaaagaac atatggcgag tttagtctat
    22501 aaactagaag aaatgggcgt tgaattggac tatcaagaag atggtattcg tgtacgtgct
    22561 gaaggggaat tacaacctgt agacatcaaa acactaccac atcctggatt cccgactgat
    22621 atgcaatcac aaatgatggc attgttatta acggcaaatg gtcataaagt cgtaaccgaa
    22681 actgtttttg aaaaccgttt tatgcatgtt gcagagttca aacgtatgaa tgctaatatc
    22741 aatgtagaag gtcgtagtgc taaacttgaa ggtaaaagtc aattgcaagg tgcacaagtt
    22801 aaagcgactg atttaagagc agcagcagcc ttaattttag ctggattagt tgctgatggt
    22861 aaaacaagcg ttactgaatt aacgcaccta gatagaggct atgttgactt acacggtaaa
    22921 ttgaagcaat taggtgcaga cattgaacgt attaacgatt aattcagtaa attaatataa
    22981 tggaggattt caaccatgga aacaattttt gattataacc aaattaaaca aattatacct
    23041 cacagacagc catttttatt aattgataaa gtagttgaat atgaagaagg tcaacgttgt
    23101 gtggctatta aacaagtatc aggaaacgaa ccattctttc aagggcattt tcctgagtat
    23161 gcggtaatgc caggcgtatt aattactgaa gcgttagctc aaacaggtgc ggtagctatt
    23221 ttaaatagtg aagaaaataa aggtaaaatc gctttatttg ctggtattga taaatgtcgt
    23281 tttaaacgtc aagtagtacc tggtgatact ttaacgttgg aagtagaaat cactaaaatt
    23341 aaaggaccaa tcggtaaagg taatgctaaa gctactgtcg atggtcaact tgcttgtagt
    23401 tgtgaactta catttgcaat tcaagatgta aaataaaaca aaaaaaacat tcaaagattt
    23461 aatgtgttgg cataatcttt gaatgttttt tattttactc ttctaatttt tcatccttta
    23521 actttggttt agactgcatc attcgattaa atgatttttt taattcttca ccagataatc
    23581 catcatcaat aagttgttct aataaacttt cagcatactg ttgacgtaaa gtgtaaatat
    23641 gacattcaaa tacgatagac attgttgttt taaaattttt aatacttttc acagtagatt
    23701 caaataaagc tggatcattg gctggacgcg tgatgacgac acgaatatcc aataactgtt
    23761 cattgtcttg atattcctta aatgtttcat aatgctcatt agaaatgaca atttcaagca
    23821 accaacccgt gccactttcc tctttattaa ttattacgcc atcttctaat tcatattcta
    23881 taattctgcc gtgttcattc acaatttgaa accttactgc tttaaaagtt ttcatattcc
    23941 cacatcccat tagtttaata tttatgattt ttgaattatt tagaagattt aaggtcattt
    24001 catgactaat ttctttaaat ataacaaata acgagatgct atagctatta attatataaa
    24061 atttgccatt ttgaccattt ttaagctagt aattaagata taggcatagg aggtgaatca
    24121 cacactacat gctaaataaa atcgtaattg tcgggagact gacgaaagac gcacaaatat
    24181 ttgaaaagga ggatagaaaa attgcaacgt tttgtgttgc aacgcaccga aattataaag
    24241 atgaaaatgg agaaatcgtc tgtgattact tattctgtaa agcatttggc aagttagctt
    24301 ctaatataga aaaatatact aatcaaggta cattggttgg tataactggt caaatgagat
    24361 caagaaagta tgataaagac ggacaaacac actttgtcac tgaattatat gttgaaacaa
    24421 taaaatttat gtcccctaaa tcccaaaata atgaaattct ctcagatagt attttagata
    24481 ttgactctca aaatatagat aatcatgact tattagaaat ttaaatccaa ttatcctaaa
    24541 catccttaat atacatttaa tccatcctaa attttttata gatctaatta acttgaaata
    24601 tactcaatta aaaacgactt acatgactag tactgttgtg taggtcgtct ttttgtgtag
    24661 tttttctaat agaaaataga catagattgc aaaacgatta cataaaaaat tgcaaataaa
    24721 aaacattttt actgtcatga caattctcga tagaatcata tctgaaatta tgggggattt
    24781 cattgtaatc actgtgtaaa aaacaaggct atttttttat tttgttgtaa cctgcacgga
    24841 ttttatagat gttacagtag tctctgtaaa acttttagag aaacaaatta cttgtaggag
    24901 gattttaaga ttatgaagaa aacattactc gcatcatcat tagcagtagg tttaggaatc
    24961 gtagcaggaa atgcaggtca cgaagcccat gcaagtgaag cggacttaaa taaagcatct
    25021 ttagcgcaaa tggcgcaatc aaatgatcaa acattaaatc aaaaaccaat tgaagctggg
    25081 gcttataatt atacatttga ctatgaaggg tttacttatc actttgaatc agatggtaca
    25141 cactttgctt ggaattacca tgcaacaggt gctaatggag caaacatgag tgcacaagca
    25201 cctgcaacta ataatgttga accatcagct gttcaagcta atcaagtaca atcacaagaa
    25261 gttgaagcac cacaaaatgc tcaaactcaa caaccacaag catcaacatc aaacaattca
    25321 caagttactg caacaccaac tgaatcaaaa gcatcagaag gttcatcagt aaatgtgaat
    25381 gctcatctaa aacaaattgc tcaacgtgaa tcaggtggca atattcatgc tgtaaatcca
    25441 acatcaggtg cagctggtaa gtatcaattc ttacaatcaa cttgggattc agtagcacct
    25501 gctaaatata aaggtgtatc accagcaaat gctcctgaaa gtgttcaaga tgccgcagca
    25561 gtaaaattat ataacactgg tggcgctgga cattgggtta ctgcataagc catttatgca
    25621 tagctaatca atagttatat aagtaacttt tagatcggaa tatatcgggg aattctcgat
    25681 atgttccggt cttttttatt tcgcaaatat acgtcattat agaattatta tttaaagata
    25741 ataatacaac tgagcaacaa taattgaaaa tgttgaaacc gtttgctaag attaatttgc
    25801 aattgaatac taaggaacta ctaggggagc ctaatgatat ggctgagatg aattgttcag
    25861 acccttatga cctgatttgg ttagtaccaa cgtaggaaag tagttattga agtgatgggg
    25921 ctagtatagc aacattgcca tcaaactact ttcatgttgg aagtagtttt tttatttagg
    25981 gaggattttt tacaatggaa ttttcacaaa aattgtacca agctgcaaag ccaatcatta
    26041 atgatattta tgaggatgac tttattcaga agatgttatc cggagatata ggagctgacg
    26101 cattacgcca ttacttaaaa gctgatgctg cgtatttaaa agaatttaca aacttatatg
    26161 ctttgttaat tccaaaaatg aatagcatga atgacgtaaa atttttagta gagcaaatag
    26221 aatttatggt tgaaggtgaa gtacttgcgc atgatatttt agcgcaaatt gttggtgaat
    26281 catacgaaga aatcattaaa acaaaagttt ggcctccaag tggcgaccat tacataaaac
    26341 atatgtattt ccaagcgcat agtcgtgaaa atgctattta tacaattgct gcaatggcac
    26401 cttgtccata tatttatgca gaattagcta agcgctctca aagtgatcat aagcttaata
    26461 gagaaaaaga tacggcaaaa tggtttgatt tttatagtac tgaaatggat gacataatta
    26521 acgtatttga ggcattaatg aataaattag ccgaatcaat gtctgacaag gaattggaac
    26581 aagtaaagca agtattttta gaaagttgca tacatgagcg tcgatttttc aatatggcta
    26641 tgacactaga acaatgggaa tttggaggaa aagtaaatga ttaaacctaa aatagcatta
    26701 accattgcag gtactgatcc aacaggtggt gccggcgtaa tggctgattt aaaatcattt
    26761 cattcatgtg gtgtatatgg tatgggcgtc gttacaagta ttgttgctca aaatacattg
    26821 ggcgtacaac atattcataa tttaaatcat caatgggtag atgaacaact tgatagtgtc
    26881 ttcaatgata ccttacctca tgctattaaa acggggatga ttgctacagc agatactatg
    26941 gaaacgattc gtcattattt aatgcaacat gaatctattc catatgtaat tgatcctgtt
    27001 atgttggcga aaagcggtga ttcattaatg gataatgaca caaagcaaaa cttgcagcat
    27061 acgttattgc cattagctga cgtagtaaca ccgaatttac cagaagctga agaaataacg
    27121 ggactaacca ttgatagtga agaaaaaatt atgcaggctg gccgcatctt tattaatgag
    27181 attggtagta aaggtatcat cattaaaggc ggtcattcaa atgatactga tatagcaaaa
    27241 gattatttat ttactaacga aggtgttcaa acatttgaaa atgaacgatt taaaacaaaa
    27301 catacgcatg gaacagggtg tacattttca gcagttataa cggcagaact tgcaaaaggt
    27361 agaccattat ttgaggctgt acacaaggct aaaaagttta tttcaatgag tatacaatat
    27421 acacctgaaa tcggccgtgg tagaggacca gtgaatcatt ttgcatattt aaagaaagag
    27481 ggattagacg atgaattatc taaataaaat acgtattgaa aacccattaa caatttgtta
    27541 tacaaacgat gtagttaaaa attttacagc gaatggttta ttaagtattg gtgcgagccc
    27601 tgccatgagt gaagctcccg aagaagctga agaattttac aaagttgcac aagcgctatt
    27661 aatcaatatc ggtactttaa cagcacaaaa tgaacaagat attattgcga ttgctcaaac
    27721 ggcaaatgag gcaggcttac ctattgtatt tgaccctgta gctgttggtg cttctacata
    27781 tcgaaagcaa ttttgtaaat tattattgaa atcagcgaaa gtatcagtaa ttaaaggcaa
    27841 tgcatctgaa atattagcgt tgattgatga tacagcaact atgaaaggta cagatagtga
    27901 tgctaatctt gatgcggttg caatagcgaa aaaggcttac gcaatatata aaactgcaat
    27961 agtaatcaca ggtaaagagg acgttattgt tcaagataat aaagccatcg tattagctaa
    28021 tggatctcca ttattagcac gagtaactgg agctggttgt ttattaggag gcgttattgc
    28081 tggattttta tttagagaaa cagaaccaga catagaagcg ttaattgaag cggtaagcgt
    28141 atttaatatt gctgctgagg tagctgctga aaatgaaaat tgtggtggtc ctggtacgtt
    28201 ttcaccattg ttgcttgata cgttatatca tttaaatgaa acaacctatc aacaacggat
    28261 tcgtattcaa gaggtggaat aatatgttta accaatcgta tctaaatgtg tattttattt
    28321 gtggtacctc cgatgttccg agtcatcgaa ctattcatga agtattagaa gcagcattaa
    28381 aggcagggat tacacttttc caatttcgag aaaagggtga atcagcttta aaaggaaacg
    28441 ataaacttgt attagctaaa gaattacagc atctctgtca tcaatatgat gtgcctttta
    28501 tcgttaatga tgatgtatcc ttggcaaaag aaattaacgc ggatggtata catgtcggtc
    28561 aagatgatgc gaaagtcaaa gagatagcac aatatttcac tgataaaatt attggactta
    28621 gtattagtga tttagatgag tatgcaaaat ctgatttaac tcatgttgat tatataggtg
    28681 ttggaccaat ctatcctacg ccgtcgaagc atgacgcgca tataccagta ggtccagaaa
    28741 tgattgcaac gtttaaagaa atgaatccgc aactaccgat tgtagctatt ggcggtatca
    28801 atactaataa tgtggcgccg attgtagaag caggtgccaa tggaatttca gtcatttcag
    28861 ccatttctaa aagtgaaaat attgaaaaga ctgttaatcg attcaaagat ttttttaata
    28921 attagttaaa tttttccact tattgtttca tacgtactca ttaatgtgat agaataaatc
    28981 ctatgtgaat ataatgatag aggtggaaaa atgaagaaaa aagcgttact accattattt
    29041 ttaggtatta tggtcttttt ggctggttgt gactattcta aacctgaaaa acgtagtggg
    29101 tttttctaca atacattcgt agatccaatg aaaaatgtat tggattggtt gggaaataac
    29161 ttattaaacg acaattatgg tttagctatt attatccttg tattggtaat tcgtattatt
    29221 ttattaccat tcatgttgtc aaactataaa aatagtcata tgatgcgtca aaaaatgaaa
    29281 gttgcaaagc cagaagttga aaaaattcaa gaaaaagtga aacgtgcgcg tacacaagaa
    29341 gaaaaaatgg ctgcaaacca agaattaatg caagtatata aaaagtatga catgaacccg
    29401 attaagagta tgttgggttg tttaccaatg ctaatccaat tgccaatcat catgggatta
    29461 tactttgtac ttaaagacca acttgtagat ggtttgttta aatatccaca cttcttatgg
    29521 ttcgatttag gacgtcctga tatttggatt acaattattg ccggtgtttt atactttatc
    29581 caagcatatg tatcaagtaa aacgatgcca gacgaacaac ggcaaatggg ttacatgatg
    29641 atggtcattt caccaattat gattatctgg atttcattaa gctcagcatc agcacttggt
    29701 ttgtactggt ctgtcagtgc ggcgttcctt gtagttcaaa cacactttgc gaacatttat
    29761 tatgaaaaag tcgctaaaaa agaagtacaa cctttcattg aagcgtatga aagagagcac
    29821 aacggcggca gcaataaaaa aggcaaaaac acacaagtag tgtctaaaaa gaaaaagaaa
    29881 taaccttagc aagtccaaat aatgtgcacc ctaaaagttg aagataattt taactttata
    29941 gggcgcacat tatttttcgc ttcaatagcg aaacttagtg tcacattata caatatgtga
    30001 taaatagaaa cagcagtaag atgatttcaa atgtgaaagt catcctactg ctgtttttat
    30061 ttgaaataat attaacgaga tttcagtatg taaaaagtgg ttaataacag agttgtcatt
    30121 cttcatcgca atgtcatctt gacacaatga ttcatcagtg tagcagtata tgtcgcaaat
    30181 ttctacagta attcactaat acatgtaaca gtgtgaaata ctaacatcac aacggcataa
    30241 tacacattgc aacgtcccaa caacatatca tagaatagta acggcatggc atcaacacca
    30301 aaccgggcta taataccata acaccgcaac gccacatcat agcactacat caagtcgcat
    30361 cgtagcacca gcacatcaaa gtaacatggc atcaacatca aaccggtcta taacaccata
    30421 acagcgcaac gccacatcat agcactacat caagtcgcat cgtggtacca gcacatcaaa
    30481 gtaacatggc atcaacgcca aaccggtcta taacaccata acaccgcaat gccacatcat
    30541 agcactacat caagacgcat cgtggtacca gcacatcaaa gtagcatggc atcgacgcca
    30601 aaccggtcta taacaccata acaccgcaat gccacatcat agcactacat caagacgcat
    30661 cgtggtacca gcacatcaaa gtaacatgac atgacaccaa gctcccacaa caacacatca
    30721 aagtaacatg gcatgacacc aagcttccac aacaacacgc cattacatcg catcatcata
    30781 ctacaacttc aatgtctagt cgtgataatt ccattccgta aaaaactgtt tcaagtacat
    30841 catcataaag tcgtgacgtt ctttagcaat catcttcgcc gtatctgtat gcattaaaga
    30901 ttctaactta agtaattttt caaagaaatg tttaattgca gatggtggca actgttcaac
    30961 taaatcatca ttaatcttat ctagtgacat atgttctgtc cacattggtt caccaaagtg
    31021 tcctgcaaat tgaaatgttc gtgcaacacc tatagcgcct atagcatcaa gacgatctgc
    31081 atccctgaca atttgacctt ctaaagataa agtgacatga tcgtttttgc cattgcgata
    31141 gctcatatta ttaataataa ataaaatatg ctcttgatct tcggttgata gtgataaaga
    31201 agataaaaat gacttcaatt caacatattg tttgttagca tctacaactt tttcgtcaac
    31261 ggtatcatga agcaaacatg cgagttcaat gactaaagta tcattaacac cttcattttc
    31321 agcgattgat ttagctaaag cagtgacacg atatacatgc gcaatatcat ggcctgtata
    31381 atcattttga tgaatagatt ccatatattt tttggcagca ctaatgacgt ctgattgttg
    31441 cattaaagtt acactcctca tatttctatt ttataagata ggtgacaata attgtgaaat
    31501 accttcttta aatttaatcc aaagacttcg cttagcataa cgtgcttttg ttaattcaga
    31561 agatactgct aaatcatcta taaaagcttg ttttaatttt ttggcaattt gttggtcata
    31621 aataaaagcg ttgacttcga aattcaatgt gaaactgcga tggtccatat tagctgttcc
    31681 cacacttgca atttcatcat ctataacaag tgtttttgag tgtaaaaagc cattgtcgta
    31741 gtgaaatact ttaacaccgg catctaataa ggatgctgca ttttttaaag tagcccaaaa
    31801 aacaaacgga tggtcaggtt tattaggaat catgatattg acatcaacac cacctaatgc
    31861 cgcaatttta atagaatcta aaaaggcttg atcaggtatg aaatagggag attgaatata
    31921 aatcgatttt ttagcagatg aaatcatttt caaatagccg tatttaatct gttcccattc
    31981 ttcgtcagga ccactagaag ctatttgaac gccaattgtt ccaccagaat ttacatctgg
    32041 gaaataacga tcatcatagg agatgtggtc acgtgtggct tgtgaattcc aatctagaat
    32101 aaatcgtaat tgcaatgcat tcactgcatc cccgacaatt cgtaaatgcg tatctcgcca
    32161 atagccgaat ttttttgatt tacctaagta ctcatcacca acattaaaac caccaacata
    32221 tccaatttgc ccatctatta caacaatttt tcgatggttt cgattgttca tacgcaagtt
    32281 aattaaaggt aattttgatg ggaaaaatgc ttcagcatgt ccacctttat tgcgaaacgg
    32341 gcgtaagcct tttttacgca gtccacgaga acccatgtca tcataaagaa ttttaacttc
    32401 tacaccttgt tccgcttttt taccaagttc atttaaaatg gtacgaccta attcatcatt
    32461 ttgaataata tagtactgaa aatgaatata atcagtagca ttacggatgt cttgtattag
    32521 gtcatcaaat ttttcttggc cgtctgtgta tatttttaaa tcgttgtctg ttgttaaaaa
    32581 tgctgcgtta ttatataaca acatttgaat catttcttta aattttacaa tttgataatt
    32641 ggaatttgaa aagttttcat tttttaaagc agctaattgc tcatcaacga ttaactctaa
    32701 tccttttt
//
LOCUS       DAOBBH010000009        31231 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.009, whole genome shotgun sequence.
ACCESSION   DAOBBH010000009 DAOBBH010000000
VERSION     DAOBBH010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 31231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31231
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.009"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            393..1730
                     /locus_tag="P6L01_000266"
     CDS             393..1730
                     /locus_tag="P6L01_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDP3029367.1"
                     /translation="MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTE
                     IPGLDDLHHPEEVILESMKQVEKHSDYDAYFLVNGTTSGILSVIQSFSQKKGDILMAR
                     NVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLAVLTYPNYYGET
                     FNVEEVIKSLHQLNIPVLIDEAHGAHFGLQGFPDSTLNYQADYVVQSFHKTLPALTMG
                     SVLYIHKNAPYRETIIEYLSYFQTSSPSYLIMASLESAAEFYKTYDSTVFFDKRAQLI
                     ECLEKKGFEMLQVDDPLKLLIKYEGFTGHDIQNWFMNAHIYLELADDYQALAILPLWH
                     HDDTYLFDLLLRKIEDMILPKKSVSKVKQTQLLTTEGNYKPKRFEYVTWCDLKKAKGK
                     VLARHIVPYPPGIPIIFKGETITENMIELVNEYLETGMIVEGIKNNKILVEDE"
     gene            1732..2349
                     /locus_tag="P6L01_000267"
     CDS             1732..2349
                     /locus_tag="P6L01_000267"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDP3029368.1"
                     /translation="MSAFITFEGPEGSGKTTVINEVYHRLVKDYDVIMTREPGGVPTG
                     EEIRKIVLEGNDMDIRTEAMLFAASRREHLVLKVIPALKEGKVVLCDRYIDSSLAYQG
                     YARGIGVEEVRALNEFAINGLYPDLTIYLNVSAEVGRERIIKNSRDQNRLDQEDLKFH
                     EKVIEGYQEIIHNESQRFKSVNADQPLENVVEDTYQTIIKYLEKI"
     gene            2377..2706
                     /locus_tag="P6L01_000268"
     CDS             2377..2706
                     /locus_tag="P6L01_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDP3029369.1"
                     /translation="MKMIIAIVQDQDSQELADQLVKNNFRATKLATTGGFLRAGNTTF
                     LCGVNDDRVDEILSVINQTCGNREQLVSPITPMGGSADSYIPYPVEVEVGGATVFVMP
                     VDAFHQF"
     gene            2920..3846
                     /locus_tag="P6L01_000269"
     CDS             2920..3846
                     /locus_tag="P6L01_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDP3029370.1"
                     /translation="MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQ
                     TDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEK
                     LTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
                     EQNMSKPVAEMISTYTTQIDNAIALNEEFDLLALRKSVIRWCELLLTNKPMALIGIID
                     LLKQAKNKKLQSLTIAAVNGFFEDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLI
                     LMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS"
     gene            3847..4650
                     /locus_tag="P6L01_000270"
     CDS             3847..4650
                     /locus_tag="P6L01_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDP3029371.1"
                     /translation="MPNVIGVQFQKAGKLEYYTPNDIQVDIEDWVVVESKRGIEIGIV
                     KNPLMDIAEEDVVLPLKNIIRIADDKDIDKFNCNERDAENALILCKDIVREQGLDMRL
                     VNCEYTLDKSKVIFNFTADDRIDFRKLVKILAQHLKTRIELRQIGVRDEAKLLGGIGP
                     CGRSLCCSTFLGDFEPVSIKMAKDQNLSLNPTKISGACGRLMCCLKYENDYYEEVRAQ
                     LPDIGEAIETPDGNGKVVALNILDISMQVKLEGHEQPLEYKLEEIETMH"
     gene            4667..5014
                     /gene="yabA"
                     /locus_tag="P6L01_000271"
     CDS             4667..5014
                     /gene="yabA"
                     /locus_tag="P6L01_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDP3029372.1"
                     /translation="MDRNEIFEKIMRLEMNVNQLSKETSELKAHAVELVEENVALQLE
                     NDNLKKVLGNDEPTTIDTANSKPAKAVKKPLPSKDNLAILYGEGFHICKGELFGKHRH
                     GEDCLFCLEVLSD"
     gene            5288..6013
                     /locus_tag="P6L01_000272"
     CDS             5288..6013
                     /locus_tag="P6L01_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDP3029373.1"
                     /translation="MLKENERFDQLIKEDFSIIQNDDVFSFSTDALLLGHFTKPRTKD
                     IVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYLTMHHMDLK
                     NVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKE
                     GGRLNMVHRADRLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEI
                     MPPFYIYNEDGNYSEEMKDVYYG"
     gene            6006..6254
                     /locus_tag="P6L01_000273"
     CDS             6006..6254
                     /locus_tag="P6L01_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDP3029374.1"
                     /translation="MDSHFVYIVKCSDGSLYTGYAKDVNARVEKHNRGQGAKYTKVRR
                     PVHLVYQEMYETKSEALKREYEIKTYTRQKKLRLIKER"
     gene            6256..7095
                     /gene="rsmI"
                     /locus_tag="P6L01_000274"
     CDS             6256..7095
                     /gene="rsmI"
                     /locus_tag="P6L01_000274"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDP3029375.1"
                     /translation="MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCN
                     HYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREA
                     NIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYE
                     SPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFV
                     ILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYH
                     QIS"
     gene            7380..9353
                     /gene="metG"
                     /locus_tag="P6L01_000275"
     CDS             7380..9353
                     /gene="metG"
                     /locus_tag="P6L01_000275"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDP3029376.1"
                     /translation="MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDV
                     RYLTGTDEHGQKIQEKAQKAGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHK
                     HVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGH
                     EVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRT
                     SFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRF
                     HSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYY
                     LMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMVNKYFDGELPAYQGPLHE
                     LDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQK
                     DMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLTES
                     IMVTGQPKPIFPRLDSEAEIAYIKESMQPPATEEEKEEIPSKPQIDIKDFDKVEIKAA
                     TIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLM
                     GQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK"
     gene            9384..10157
                     /locus_tag="P6L01_000276"
     CDS             9384..10157
                     /locus_tag="P6L01_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDP3029377.1"
                     /translation="MLIDTHVHLNDEQYDDDLSEVITRAREAGVDRMFVVGFNKSTIE
                     RAMKLIDEYDFLYGIIGWHPVDAIDFTEEHLEWIESLAQHPKVIGIGEMGLDYHWDKS
                     PADVQKEVFRKQIALAKRLKLPIIIHNREATQDCIDILLEEHAEEVGGIMHSFSGSPE
                     IADIVTNKLNFYISLGGPVTFKNAKQPKEVAKHVSMERLLVETDAPYLSPHPYRGKRN
                     EPARVTLVAEQIAELKGLSYEEVCEQTTKNAEKLFNLNS"
     gene            10324..10860
                     /gene="rnmV"
                     /locus_tag="P6L01_000277"
     CDS             10324..10860
                     /gene="rnmV"
                     /locus_tag="P6L01_000277"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDP3029378.1"
                     /translation="MKINEFIVVEGRDDTERVKRAVECDTIETNGSAINEQTLEVIRN
                     AQQSRGVIVLTDPDFPGDKIRSTITEHVKGVKHAYIDREKAKNKKGKIGVEHADLIDI
                     KEALMHVSSPFDEAYESIDKSVLIELGLIVGKDARRRREILSRKLRIGHSNGKQLLKK
                     LNAFGYTEADVRQALEDE"
     gene            10871..11764
                     /gene="rsmA"
                     /locus_tag="P6L01_000278"
     CDS             10871..11764
                     /gene="rsmA"
                     /locus_tag="P6L01_000278"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDP3029379.1"
                     /translation="MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIID
                     ASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVIN
                     EDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
                     GERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLV
                     TVDNEEAFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGET
                     LSIQDFAKLYEEKKKFPQLEN"
     gene            11864..12127
                     /locus_tag="P6L01_000279"
     CDS             11864..12127
                     /locus_tag="P6L01_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDP3029380.1"
                     /translation="MPKSILDIKNSIDCHVGNRIVLKANGGRKKTIKRSGILKETYPS
                     VFIVELDQDKHNFERVSYTYTDVLTENVQVSFEEDNHHESIAH"
     gene            12437..13285
                     /locus_tag="P6L01_000280"
     CDS             12437..13285
                     /locus_tag="P6L01_000280"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDP3029381.1"
                     /translation="MIYETAPAKINFTLDTLFKRNDGYHEIEMIMTTVDLNDRLTFHK
                     RKDRKIVVEIEHNYVPSNHKNLAYRAAQLFIEQYQLKQGVTISIDKEIPVSAGLAGGS
                     ADAAATLRGLNRLFDIGASLEELALLGSKIGTDIPFCIYNKTALCTGRGEKIEFLNKP
                     PSAWVILAKPNLGISSPDIFKLINLDKRYDVHTKMCYEALENRDYQQLCQSLSNRLEP
                     ISVSKHPQIDKLKNNMLKSGADGALMSGSGPTVYGLARKESQAKNIYNAVNGCCNEVY
                     LVRLLG"
     gene            13299..14123
                     /gene="purR"
                     /locus_tag="P6L01_000281"
     CDS             13299..14123
                     /gene="purR"
                     /locus_tag="P6L01_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDP3029382.1"
                     /translation="MRYKRSERIVFMTQYLMNHPNKLIPLTFFVKKFKQAKSSISEDV
                     QIIKNTFQKEKLGTVITTAGASGGVTYKPMMSKEEATEVVNEVITLLEEKERLLPGGY
                     LFLSDLVGNPSLLNKVGKLIASIYMEEKLDAVVTIATKGISLANAVANILNLPVVVIR
                     KDNKVTEGSTVSINYVSGSSRKIETMVLSKRTLAENSNVLVVDDFMRAGGSINGVMNL
                     MNEFKAHVKGVSVLVESKEVKQRLIEDYTSLVKLSDVDEYNQEFNVEPGNSLSKFS"
     gene            14140..14520
                     /locus_tag="P6L01_000282"
     CDS             14140..14520
                     /locus_tag="P6L01_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDP3029383.1"
                     /translation="MKIINTTRLPEALGPYSHATVVNGMVYTSGQIPLNVDGKIVSAD
                     VQAQTKQVLENLKVVLEEAGSDLNSVAKATIFIKDMNDFQKINEVYGQYFNEHKPARS
                     CVEVARLPKDVKVEIELVSKIKEL"
     gene            14592..14894
                     /gene="spoVG"
                     /locus_tag="P6L01_000283"
     CDS             14592..14894
                     /gene="spoVG"
                     /locus_tag="P6L01_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HDP3029384.1"
                     /translation="MKVTDVRLRKIQTDGRMKALVSITLDEAFVIHDLRVIEGNSGLF
                     VAMPSKRTPDGEFRDIAHPINSDMRQEIQDAVMKVYDETDEVVPDKNATSEDSEEA"
     gene            15105..15236
                     /locus_tag="P6L01_000284"
     CDS             15105..15236
                     /locus_tag="P6L01_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029385.1"
                     /translation="MRLNDARIFEVRKKLFLKLQRIKNNAFYMLKEYCRLNYNNDEV"
     gene            15259..16611
                     /gene="glmU"
                     /locus_tag="P6L01_000285"
     CDS             15259..16611
                     /gene="glmU"
                     /locus_tag="P6L01_000285"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDP3029386.1"
                     /translation="MRRHAIILAAGKGTRMKSKKYKVLHEVAGKPMVEHVLESVKGSG
                     VDQVVTIVGHGAESVKGHLGERSLYSFQEKQLGTAHAVQMAKSHLEDKEGTTIVVCGD
                     TPLITKETLETLIAHHEDANAQATVLSASIQQPYGYGRIVRNASGRLERIVEEKDATQ
                     AEKDINEISSGIFAFNNKTLFEKLTQVKNDNAQGEYYLPDVLSLILNDGGIVEVYRTN
                     DVEEIMGVNDRVMLSQAEKAMQRRTNHYHMLNGVTIIDPDSTFIGPDVTIGSDTVIEP
                     GVRINGRTEIGEDVVIGQYSEINNSTIENGACIQQSVVNDASVGANTKVGPFAQLRPG
                     AQLGADVKVGNFVEIKKADLKDGAKVSHLSYIGDAVIGERTNIGCGTITVNYDGENKF
                     KTIVGKDSFVGCNVNLVAPVTIGDDVLVAAGSTITDDVPNDSLAVARARQTTKEGYRK
                     "
     gene            16758..17723
                     /locus_tag="P6L01_000286"
     CDS             16758..17723
                     /locus_tag="P6L01_000286"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDP3029387.1"
                     /translation="MLNNEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSD
                     GEIQINIEESIRGCDVFIIQPTSYPVNLHLMELLIMIDACKRASAATINIVVPYYGYA
                     RQDRKARSREPITAKLVANLIETAGATRMIALDLHAPQIQGFFDIPIDHLMGVPILAK
                     HFKDDPNINPEECVVVSPDHGGVTRARKLADILKTPIAIIDKRRPRPNVAEVMNIVGE
                     IEGRTAIIIDDIIDTAGTITLAAQALKDKGAKEVYACCTHPVLSGPAKERIENSAIKE
                     LIVTNSIHLDEDRKPSNTKELSVAGLIAQAIIRVYERESVSVLFD"
     gene            17873..18526
                     /locus_tag="P6L01_000287"
     CDS             17873..18526
                     /locus_tag="P6L01_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDP3029388.1"
                     /translation="MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVD
                     EVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTV
                     EVPVQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVK
                     VTGDFKIENDSAESVVTVVAPTEEPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE"
     gene            18837..19409
                     /locus_tag="P6L01_000288"
     CDS             18837..19409
                     /locus_tag="P6L01_000288"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDP3029389.1"
                     /translation="MKCIVGLGNIGKRFELTRHNIGFEVVDYILEKNNFSLDKQKFKG
                     AYTIERMNGDKVLFIEPMTMMNLSGEAVAPIMDYYNVNPEDLIVLYDDLDLEQGQVRL
                     RQKGSAGGHNGMKSIIKMLGTDQFKRIRIGVGRPTNGMTVPDYVLQRFSNDEMVTMEK
                     VIEHAARAIEKFVETSRFDHVMNEFNGEVK"
     gene            19409..22915
                     /gene="mfd"
                     /locus_tag="P6L01_000289"
     CDS             19409..22915
                     /gene="mfd"
                     /locus_tag="P6L01_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDP3029390.1"
                     /translation="MTILTTLIKEDNHFQDLNQVFGQANTLVTGLSPSAKVTMIAEKY
                     AQSNQQLLLITNNLYQADKLETDLLQFIDAEELYKYPVQDIMTEEFSTQSPQLMSERI
                     RTLTALAQGKKGLFIVPLNGLKKWLTPVEMWQNHQMTLRVGEDIDVDQFLNKLVNMGY
                     KRESVVSHIGEFSLRGGIIDIFPLIGEPIRIELFDTEIDSIRDFDVETQRSKDNIEEV
                     DITTASDYIITEEVIRHLKEELKTAYENTRPKIDKSVRNDLKETYESFKLFESTYFDH
                     QILRRLVAFMYETPSTIIEYFQKDAIIAVDEFNRIKETEESLTVESDSFISNIIESGN
                     GFIGQSFIKYDDFETLIEGYPVTYFSLFATTMPIKLNHIIKFSCKPVQQFYGQYDIMR
                     SEFQRYVNQNYHIVVLVETETKVERMQAMLSEMHIPSITKLHRSMSSGQAVIIEGSLS
                     EGFELPDMGLVVITERELFKSKQKKQRKRTKAISNAEKIKSYQDLNVGDYIVHVHHGV
                     GRYLGVETLEVGQTHRDYIKLQYKGTDQLFVPVDQMDQVQKYVASEDKTPKLNKLGGS
                     EWKKTKAKVQQSVEDIAEELIDLYKEREMAEGYQYGEDTAEQTTFELDFPYELTPDQA
                     KSIDEIKDDMQKSRPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQ
                     HYETLIERMQDFPVEIQLMSRFRTPKEIKQTKEGLKTGFVDIVVGTHKLLSKDIQYKD
                     LGLLIVDEEQRFGVRHKERIKTLKHNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPP
                     ENRFPVQTYVLEQNMSFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQMLMPDANI
                     AVAHGQMTERDLEETMLSFINNEYDILVTTTIIETGVDVPNANTLIIEDADRFGLSQL
                     YQLRGRVGRSSRIGYAYFLHPANKVLTETAEDRLQAIKEFTELGSGFKIAMRDLNIRG
                     AGNLLGKQQHGFIDTVGFDLYSQMLEEAVNEKRGIKEPESEVPEVEVDLNLDAYLPTE
                     YIANEQAKIEIYKKLRKTETFDQIIDIKDELIDRFNDYPVEVARLLDIVEIKVHALHS
                     GITLIKDKGKIIDIHLSVKATENIDGEVLFKATQPLGRTMKVGVQNNAMTITLTKQNQ
                     WLDSLKFLVKCIEESMRISDEA"
     gene            22905..24431
                     /locus_tag="P6L01_000290"
     CDS             22905..24431
                     /locus_tag="P6L01_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDP3029391.1"
                     /translation="MKHKEAFNGVVVLTAALIVIKILSAVYRIPYQNILGDTGLYAYQ
                     QVYPIVALGMILSMNAIPSAITQNIGKYHSDEAYAKAVAYIQLVGILLFIAIFVFANN
                     IAHMMGDSHLTPMIQAASLSFIFIGMLGVLRGYYQSANNMTVPAISQVIEQVIRVGII
                     IVTIVIFVDRGWTIYEAGTIAILASTIGFLGSSIYLVAHRPFKFKMVNNTAKIVWKQF
                     ALSVLIFAISQLIVILWQVIDSVTIIKSLQAIRVPFDVAITEKGVYDRGASFIQMGLI
                     VTTTFSFALIPLLSDAIKMNNQVLMNRYANASLKITILISTAAGIGLINLLPLMNGVF
                     FKTNDLTLTLSVYMITVICVSLIMMDMALLQAQHAVRPIFVGMTAGLVIKFILNIILI
                     RLSGIIGASISTVVSLIIFGTIIHIAVTRKYHLHAMRRFFINVVLGMVFMSIVVQCVL
                     NIVTTHGRFTGLIELLCAAVLGIIALFFYIFRFNVLTYKELTYLPFGSKLYQIKKGRR
                     "
     gene            24431..25624
                     /locus_tag="P6L01_000291"
     CDS             24431..25624
                     /locus_tag="P6L01_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDP3029392.1"
                     /translation="MAHTITIVGLGNYGIDDLPLGIYKFLKTQDKVYARTLDHPVIES
                     LQDELTFQSFDHVYEAHDQFEDVYNDIVAQLVEAANEKDIVYAVPGHPRVAETTTVKL
                     LALAKDNTDIDVKVLGGKSFIDDVFEAVNVDPNDGFTLLDATSLQEVILNVRTHTLIT
                     QVYSAMVAANLKITLMERYPDDYPVQIVTGARSDGADNVVTCPLYELDHDENAFNNLT
                     SVFVPKIITSTYLYHDFDFATEVIDTLVDEDKGCPWDKVQTHETLKRYLLEETFELFE
                     AIDNEDDWHMIEELGDILLQVLLHTSIGKKEGYIDIKEVITSLNAKMIRRHPHIFGDA
                     NAETIDDLKEIWSKAKDAEGKQPRVKFEKVFAEHFLNLYEKTKDKSFDEASLKQWLEK
                     GESNT"
     gene            25621..25884
                     /locus_tag="P6L01_000292"
     CDS             25621..25884
                     /locus_tag="P6L01_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDP3029393.1"
                     /translation="MRLDKYLKVSRLIKRRTLAKEVSDQGRITINGNVAKAGSDVKVE
                     DVLTIRFGQKLVTVKVTALNEHASKDNAKGMYEIIEERRLEEA"
     gene            25902..26294
                     /locus_tag="P6L01_000293"
     CDS             25902..26294
                     /locus_tag="P6L01_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDP3029394.1"
                     /translation="MKNKVEHIENQYTSQENKKKQRQKMKMRVVRRRITVFAGVLLAI
                     IVVLSILLVVQKHRNDIDAQERKAKEAQFQKQQNEEIALKEKLNNLNDKDYIEKIARD
                     DYYLSNKGEVIFRLPEDKDSSSSKSSKK"
     gene            26399..26800
                     /locus_tag="P6L01_000294"
     CDS             26399..26800
                     /locus_tag="P6L01_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDP3029395.1"
                     /translation="MSIEVGNKLKGKVTGIKKFGAFVELPEGKSGLVHISEVADNYVE
                     NVEEHLSVGDEVDVKVLSIADDGKISLSIKKAKDRPRRQHTSKPSHQKPVQKAEDFEK
                     KLSNFLKDSEDKLTSIKRQTESRRGGKGSRR"
     gene            26980..28275
                     /gene="tilS"
                     /locus_tag="P6L01_000295"
     CDS             26980..28275
                     /gene="tilS"
                     /locus_tag="P6L01_000295"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDP3029396.1"
                     /translation="MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTC
                     LHVNHGVRSASIEEARFLEAYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWF
                     DEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
                     VSKKEIKQFQERYHIPYFEDESNKDNKYIRNDIRNRIIPAIDENNQLKVSHLLKLKQW
                     HDEQYDILQYSAKQFIQEFVKFDEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYEL
                     FNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIKK
                     PGNYFFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQW
                     VRDQMPIVLDKQQRIIAVGDLYQQQTIKKWIIISKNGDE"
     gene            28280..28819
                     /gene="hpt"
                     /locus_tag="P6L01_000296"
     CDS             28280..28819
                     /gene="hpt"
                     /locus_tag="P6L01_000296"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDP3029397.1"
                     /translation="MHNDLKEVLLTEEDIQNICKELGAQLTKDYQGKPLVCVGILKGS
                     AMFMSDLIKRIDTHLSIDFMDVSSYHGGTESTGEVQIIKDLGSSIENKDVLIIEDILE
                     TGTTLKSITELLQSRKVNSLEIVTLLDKPNRRKADIEAKYVGKKIPDEFVVGYGLDYR
                     ELYRNLPYIGTLKPEVYSN"
     gene            29076..31169
                     /gene="ftsH"
                     /locus_tag="P6L01_000297"
     CDS             29076..31169
                     /gene="ftsH"
                     /locus_tag="P6L01_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDP3029398.1"
                     /translation="MQKAFRNVLVIVIIGVIIFGLFSYLNGNGNMPKQLTYNQFTEKL
                     EKGDLKTLEIQPQQNVYMVSGKTKNDEDYSSTILYNNEKELQKITDAAKKQNGVKLTI
                     KEEEKQSVFVSILSTLIPVVVIALLFIFFLSQAQGGGSGGRMMNFGKSKAKMYDNNKR
                     RVRFSDVAGADEEKQELIEIVDFLKDNKKFKEMGSRIPKGVLLVGPPGTGKTLLARAV
                     AGEAGAPFFSISGSDFVEMFVGVGASRVRDLFDNAKKNAPCIIFIDEIDAVGRQRGAG
                     VGGGHDEREQTLNQLLVEMDGFGENEGIIMIAATNRPDILDPALLRPGRFDRQIQVGR
                     PDVKGREAILHVHAKNKPLDETVDLKAISQRTPGFSGADLENLLNEASLIAVREGKKK
                     IDMRDIEEATDRVIAGPAKKSRVISKKERNIVAHHEAGHTIIGMVLDEAEVVHKVTIV
                     PRGQAGGYAMMLPKQDRFLMTEQELLDKICGLLGGRVSEDINFNEVSTGASNDFERAT
                     QIARSMVTQYGMSKKLGPLQFGHSNGQVFLGKDMQGEPNYSSQIAYEIDKEVQRIVKE
                     QYERCKQILLEHKEQLILIAETLLTEETLVAEQIQSLFYEGKLPEIDYDAAKVVKDED
                     SEFNDGKFGKSYEEIRKEQLEDGQRDESEDRKEEKDIAEDKKEADKSDEKDEPAHRQA
                     PNIEKPYDPNHPDNK"
BASE COUNT        11293 a         4203 c         6227 g         9508 t
ORIGIN      
        1 ggatggtagc caacttacgt tccgctagag tagaacgttg ctagacaaga aatgaatgcg
       61 atgagccgca ttgagtgtga aattgatatt ttaataatgt gcacttttga ttatttaaat
      121 ctgtgactag aagtataagt gaagacattc agaagtatta taaaaagtga acagcagtaa
      181 gatagttttt aatcataaat catcttactg ctgtttttag attttatgtc taatatcttt
      241 taaatcgaag tacaaaaagg aaattaatta ttatacaata gacaagctat tgcataagta
      301 acactaactt ttgtcaaaga agtgttacta tataattaat acttttgaaa gtaactaatt
      361 ccaaaacagt gtaaataaag gaagcgtata tcatgaagca acctatttta aataaattag
      421 aaagtttaaa tcaagaagaa gcgatttctt tgcatgttcc gggtcataaa aatatgacta
      481 tcggtcattt atctcaatta tcaatgacaa tggataaaac tgaaatacct ggattagatg
      541 atttacatca tcctgaagaa gtcattttgg aaagtatgaa gcaggtggag aaacattcag
      601 attatgatgc ttatttctta gtgaatggca ccacttcagg aatattatct gtcatccagt
      661 ctttttcaca gaaaaaaggc gatatcttaa tggcaagaaa tgtacataaa tctgtgttac
      721 atgcgctcga tattagccaa caagaagggc attttattga aacgcatcaa agtccgttaa
      781 cgaatcatta taataaagtt aatttaagcc gtttgaataa tgacggtcac aaacttgctg
      841 tgttgactta tcctaactat tacggtgaaa catttaatgt agaagaggtt atcaaatctt
      901 tgcaccaatt aaatattcct gtactcattg acgaagcaca cggcgcgcac tttggattgc
      961 aaggatttcc agattctaca ttaaattatc aagctgacta tgttgttcaa tcttttcata
     1021 aaacgttacc agctttaacg atgggctcgg tactttatat tcataaaaat gcaccttata
     1081 gagaaactat tatagaatat ctaagctact tccaaacatc tagtccttcg tatttgatta
     1141 tggctagttt agagtcagct gccgagttct ataaaacata tgatagtacc gtgttttttg
     1201 ataagagagc gcaattaatc gaatgtttgg agaagaaggg ttttgaaatg cttcaagttg
     1261 atgatccgtt gaagttgctg ataaaatatg aaggttttac aggtcatgat attcaaaatt
     1321 ggtttatgaa tgcacatatc tatttagaat tagcggacga ctatcaagca ttagcgatat
     1381 tgccgttatg gcatcatgat gatacgtatt tatttgattt gcttttacgt aaaattgaag
     1441 atatgatttt accgaaaaaa tcagtttcta aagttaaaca aacacaactt ttaacaactg
     1501 aaggtaacta taaaccaaaa cgctttgaat atgttacttg gtgtgatttg aaaaaggcaa
     1561 aaggtaaagt tctggcgcga catattgtcc cgtatccgcc agggattcct attattttca
     1621 aaggagaaac aataactgaa aatatgatag aattggtaaa tgaatatctg gaaactggaa
     1681 tgatagttga aggaattaaa aataataaaa ttttagttga ggatgaataa aatgtcagct
     1741 tttataactt ttgagggccc agaaggctct ggaaaaacaa ctgtaattaa tgaagtttac
     1801 catagattag taaaagatta tgatgtcatt atgactagag aaccaggtgg tgttcctact
     1861 ggtgaagaaa tacgtaaaat tgtattagaa ggcaatgata tggacattag aactgaagca
     1921 atgttatttg ctgcatctag aagagaacat cttgtattaa aggtcatacc agctttaaaa
     1981 gaaggtaagg ttgtgttgtg tgatcgctat atcgatagtt cattagctta tcaaggttat
     2041 gctagaggga ttggcgttga agaagtaaga gcattaaacg aatttgcaat aaatggatta
     2101 tatccagact tgacgattta tttaaatgtt agtgctgaag taggtcgcga acgtattatt
     2161 aaaaattcaa gagatcaaaa tagattagat caagaagatt taaagtttca cgaaaaagta
     2221 attgaaggtt accaagaaat cattcataat gaatcacaac ggttcaaaag cgttaatgca
     2281 gatcaacctc ttgaaaatgt tgttgaagac acgtatcaaa ctatcatcaa atatttagaa
     2341 aagatatgat ataattgtta gaagaggtgt tataaaatga aaatgattat agcgatcgta
     2401 caagatcaag atagtcagga acttgcagat caacttgtta aaaataactt tagagcaaca
     2461 aaattggcaa caacaggtgg gtttttaaga gcgggtaata caacattctt atgtggtgtc
     2521 aatgatgacc gtgtagatga aatattgtct gtgattaatc aaacgtgtgg taatagagaa
     2581 cagttggttt cacctattac acctatggga ggcagtgcgg attcgtacat tccatatcca
     2641 gttgaagttg aagttggcgg tgctactgta tttgttatgc cagttgatgc attccatcaa
     2701 ttttaattct ataatacaat catcaattag agatacttaa aatagtgtat taataagtta
     2761 ttaacaattt tgggttgctt gactgcgact agttcagatg ccaatagatt tgatttttgt
     2821 ggttctaaaa ataatcacaa atcatggtcg ctattgttgc agtaattagt tgctctttgg
     2881 caaccttttt atataaaagc aaaagggagt ttgtaatgaa tggatgaaca gcaacaattg
     2941 acgaatgcat atcattcaaa taaattatcg catgcctatt tatttgaagg tgatgatgca
     3001 caaacgatga aacaagttgc gattaatttt gcaaagctta ttttatgtca aacagatagt
     3061 caatgtgaaa caaaggttag tacatataat catccagact ttatgtatat atcaacaact
     3121 gagaatgcaa ttaagaaaga acaagttgaa caacttgtgc gtcatatgaa tcaacttcct
     3181 atagaaagca caaataaagt gtacatcatt gaagactttg aaaagttaac tgttcaaggg
     3241 gaaaacagta tcttgaaatt tcttgaagaa ccaccggaca atacgattgc tattttattg
     3301 tctacaaaac ctgagcaaat tttagacaca atccattcaa ggtgtcagca tgtgtatttc
     3361 aagcctattg ataaagaaaa gtttataaat agattagttg aacaaaacat gtctaagcca
     3421 gtagctgaaa tgattagtac ttatactacg caaatagata atgcaatagc tttaaatgaa
     3481 gaatttgatt tattagcatt aaggaaatca gttatacgtt ggtgtgaatt gttgcttact
     3541 aacaagccaa tggcacttat aggtattatt gatttattga aacaggctaa aaataaaaaa
     3601 ctgcaatctt taactattgc agctgtgaat ggtttcttcg aagatatcat acatacaaag
     3661 gtaaatgtag aggataaaca aatatatagt gatttaaaaa atgatattga tcaatatgcg
     3721 caaaagttgt cgtttaatca attaattttg atgtttgatc aactgacgga agcacataag
     3781 aaattgaatc aaaatgtaaa tccaacgctt gtatttgaac aaatcgtaat taagggtgtg
     3841 agttagatgc caaatgtaat aggtgttcag tttcaaaaag cgggaaaatt agaatattat
     3901 acacctaatg atatacaagt agatatagaa gactgggtag ttgtcgaatc taaaagaggc
     3961 atagagatag gtattgttaa aaatccatta atggatattg ctgaagagga tgttgtgtta
     4021 cctcttaaaa atattattcg cattgctgat gacaaagata ttgataaatt taattgtaat
     4081 gaacgagatg ctgaaaatgc attaatacta tgtaaagaca ttgtaagaga acaaggtttg
     4141 gacatgcgtt tagtcaattg cgaatataca ttagataaat cgaaagttat ttttaatttt
     4201 acggcggatg atcgtattga ttttagaaaa ttagtaaaaa tattagcgca acatttaaaa
     4261 acacgtatcg agttgagaca aattggtgta agggatgaag ccaaattgct tggcggtatc
     4321 ggaccttgtg gtaggtcgtt atgttgttct acatttttag gggattttga accagtatcg
     4381 attaagatgg ctaaggatca aaatttatca ttaaatccaa ctaaaatttc tggtgcatgt
     4441 ggtcgtttga tgtgttgttt aaaatatgaa aatgactatt atgaggaagt acgtgcacaa
     4501 ttacctgata ttggtgaagc aattgaaacg cctgatggta acgggaaagt agttgcttta
     4561 aatatattag acatttctat gcaggtgaag cttgagggac atgaacagcc acttgaatat
     4621 aaattagaag aaatagaaac tatgcattaa ggaggcatta ttacatttgg atcgcaatga
     4681 aatatttgaa aaaataatgc gtttagaaat gaatgtcaat caactttcaa aggaaacttc
     4741 agaattaaag gcacatgcag ttgaattagt agaagaaaat gtagcgcttc aacttgaaaa
     4801 tgataatttg aaaaaggtgt tgggcaatga tgaaccaact actattgata ctgcgaattc
     4861 aaaaccagca aaagctgtga aaaagccatt accaagtaaa gataatttgg ctatattgta
     4921 tggagaagga tttcatattt gtaaaggcga attatttgga aaacatcgac atggtgaaga
     4981 ttgtctgttc tgtttagaag ttttaagtga ttaatcaagc acactcaaat agtgttataa
     5041 ttataaatga atatggtttg gataagtctg agacaatgca tgtttcaggc tttaattgtg
     5101 tataaagttt tggtgattgc ataagagatg gcggtactaa atgttattat taagtgtgca
     5161 cgcagtatca ttagttataa aatgtagctg ttaaaagtca aaaatacatc gaatgtagtt
     5221 aggcatataa tataaaaaga gatttcaatt actcaataga aaaaggttgt cttcatagga
     5281 gttaaaaatg ttaaaagaga atgaacgatt tgatcaacta atcaaagaag attttagtat
     5341 tattcaaaat gatgatgttt tttcgttttc aacggatgct ttgttgttag ggcattttac
     5401 aaaacctaga acaaaagata ttgtgttgga cttatgttca ggcaatgggg tgataccctt
     5461 gttattgttt gcgaaacatc cacgacatat agaaggtgtt gagattcaaa aaacacttgt
     5521 cgatatggcg cgacgcacat ttcaattcaa tgatgttgat gaatatttaa caatgcatca
     5581 catggatttg aaaaacgtta ctaaagtatt taaaccttca caatatactt tagtaacgtg
     5641 taatccgcct tattttaaag agaatcagca acaccaacat caaaaagaag cacataagat
     5701 agcgagacat gagattatgt gtacacttga agattgcatg attgcagccc gtcatttatt
     5761 aaaagaaggt ggcaggctaa atatggtaca tcgtgcagat agactaatgg atgtcttgtt
     5821 tgaaatgaga aaagtgaata ttgaacctaa gaaagtcgtt tttatatata gtaaggtagg
     5881 gaaatcagca caaacgatag tagtagaagg tcgaaaaggt ggaaatcaag gtttagaaat
     5941 catgccccca ttttatattt ataatgaaga tggtaattat agcgaagaaa tgaaggacgt
     6001 atattatgga tagtcatttt gtatatattg taaaatgtag tgatggaagt ttatatacag
     6061 gatacgctaa agacgttaat gcacgtgttg aaaaacataa ccgaggtcaa ggagccaaat
     6121 atacgaaagt aagacgtccg gtgcatttag tttatcaaga aatgtatgag acaaagtctg
     6181 aagcattgaa gcgtgaatat gaaattaaaa cttataccag acaaaagaaa ttgcgattaa
     6241 ttaaggagcg atagtatggc tgtattatat ttagtgggca caccaattgg taatttagca
     6301 gatattactt atagagcagt tgatgtattg aaacgtgttg atatgattgc ttgtgaagac
     6361 actagagtaa ctagtaagct gtgtaatcat tatgatattc caactccatt aaagtcatat
     6421 cacgaacata acaaggataa gcagactgct tttatcattg aacagttaga attaggtctt
     6481 gacgttgcgc tcgtatctga tgctggattg cccttaatta gtgatcctgg atacgaatta
     6541 gtagtggcag ccagagaagc taatattaaa gtagagactg tgcctggacc taatgctggg
     6601 ctgacggctt tgatggctag tggattacct tcatatgtat atacattttt aggatttttg
     6661 ccacgaaaag agaaagaaaa aagtgctgta ttagagcaac gtatgcatga aaatagcaca
     6721 ttaattatat acgaatcacc gcatcgtgtg acagatacat taaaaacaat tgcaaagata
     6781 gatgcaacac gacaagtatc actagggcgt gaattaacta agaagttcga acaaattgta
     6841 actgatgatg taacacaatt acaagcattg attcagcaag gcgatgtacc attgaaaggc
     6901 gaattcgtta tcttaattga aggtgctaaa gcgaacaatg agatatcgtg gtttgatgat
     6961 ttatctatca atgagcatgt tgatcattat attcaaactt cacagatgaa accaaaacaa
     7021 gctattaaaa aagttgctga agaacgacaa cttaaaacga atgaagtata taatatttat
     7081 catcaaataa gttaatcact ttatcgatta tatgaaattt taaacgattt tataaacgca
     7141 agctgtaatt ttaaatggta agttatcatt ttgcattgat actgataaaa tgatgttgac
     7201 tatgataaaa aaatgatgac atcgacgttt tttaatgtaa aataaataca ttgaaagtaa
     7261 taaatacctt aacattgaat aagatgaaaa tgagatgacg agataaatgt tcgcgtccgt
     7321 tgaaatgcat agaaatctta gatattattt gaagtgagac attacgagga ggaacagtta
     7381 tggctaaaga aacattttat ataacaaccc caatatacta tcctagtggg aatttacata
     7441 taggacatgc atattctaca gtggctggag atgttattgc aagatataag agaatgcaag
     7501 gatatgatgt tcgttatttg actggaacgg atgaacacgg tcaaaaaatt caagaaaaag
     7561 ctcaaaaagc tggtaagaca gaaattgaat atttggatga gatgattgct ggaattaaac
     7621 aattgtgggc taagcttgaa atttcaaatg atgattttat cagaacaact gaagaacgtc
     7681 ataaacatgt cgttgagcaa gtgtttgaac gtttattaaa gcaaggtgat atctatttag
     7741 gtgaatatga aggttggtat tctgttccgg atgaaacata ctatacagag tcacaattag
     7801 tagacccaca atacgaaaac ggtaaaatta ttggtggcaa aagtccagat tctggacacg
     7861 aagttgaact agttaaagaa gaaagttatt tctttaatat tagtaaatat acagaccgtt
     7921 tattagagtt ctatgaccaa aatccagatt ttatacaacc accatcaaga aaaaatgaaa
     7981 tgattaacaa cttcattaaa ccaggacttg ctgatttagc tgtttctcgt acatcattta
     8041 actggggtgt ccatgttccg tctaatccaa aacatgttgt ttatgtttgg attgatgcgt
     8101 tagttaacta tatttcagca ttaggctatt tatcagatga tgagtcacta tttaacaaat
     8161 actggccagc agatattcat ttaatggcta aggaaattgt gcgattccac tcaattattt
     8221 ggcctatttt attgatggca ttagacttac cgttacctaa aaaagtcttt gcacatggtt
     8281 ggattttgat gaaagatgga aaaatgagta aatctaaagg taatgttgta gaccctaata
     8341 ttttaattga tcgctatggt ttagatgcta cacgttatta tctaatgcgt gaattaccat
     8401 ttggttcaga tggcgtattt acacctgaag catttgttga gcgtacaaat ttcgatctag
     8461 caaatgactt aggtaactta gtaaaccgta cgatttctat ggttaataag tactttgatg
     8521 gcgaattacc agcgtatcaa ggtccacttc atgaattaga tgaagaaatg gaagctatgg
     8581 ctttagaaac agtgaaaagc tacactgaaa gcatggaaag tttgcaattt tctgtggcat
     8641 tatctacggt atggaagttt ataagtagaa cgaataagta tattgacgaa acaacgcctt
     8701 gggtattagc taaggacgat agccaaaaag atatgttagg caatgtaatg gctcacttag
     8761 ttgaaaatat tcgttatgca gctgtattat tacgtccatt cttaacacat gcgccgaaag
     8821 agatttttga acaattgaac ataaacaatc ctcaatttat ggaatttagt agtttagagc
     8881 aatatggtgt gcttactgag tcaattatgg ttactgggca acctaaacct attttcccaa
     8941 gattggatag cgaagcggaa attgcatata tcaaagaatc aatgcaaccg cctgctactg
     9001 aagaggaaaa agaagagatt cctagcaaac ctcaaattga tattaaagac tttgataaag
     9061 ttgaaattaa ggcagcaacg attattgatg ctgaacatgt taagaagtca gataagcttt
     9121 taaaaattca agtagactta gattctgaac aaagacaaat tgtatcagga attgccaaat
     9181 tctatacacc agatgatatt attggtaaaa aagtagcagt tgttactaac ctgaaaccag
     9241 ctaaattaat gggacaaaaa tctgaaggta tgatattatc tgctgaaaaa gatggtgtat
     9301 taaccttagt aagtttacca agtgcaattc caaatggtgc agtgattaaa taactgtatt
     9361 tttaaaaatt aggagagata attatgttaa tcgatacaca tgtccattta aatgatgagc
     9421 aatacgatga tgatttgagt gaagtgatta cacgtgctag agaagcaggt gttgatcgta
     9481 tgtttgtagt tggttttaac aaatcgacaa ttgaacgcgc gatgaaatta atcgatgagt
     9541 atgatttttt atatggcatt atcggttggc atccagttga cgcaattgat tttacagaag
     9601 aacacctgga atggattgaa tctttagctc agcatccaaa agtgattggt attggtgaaa
     9661 tgggattaga ttatcactgg gataaatctc ctgcagatgt tcaaaaggaa gtttttagaa
     9721 agcaaattgc tttagctaag cgtttgaagt taccaattat cattcataac cgtgaagcaa
     9781 ctcaagactg tatcgatatc ttattggagg agcatgctga agaggtaggc gggattatgc
     9841 atagctttag tggttctcca gaaattgcag atattgtaac taataagctg aatttttata
     9901 tttcattagg tggacctgtg acatttaaaa atgctaaaca gcctaaagaa gttgctaagc
     9961 atgtgtcaat ggagcgtttg ctagttgaaa ccgatgcacc gtatctctcg ccacatccgt
    10021 atagagggaa gcgaaatgaa ccggcgagag taactttagt agctgaacaa attgctgaat
    10081 taaaaggctt atcttatgaa gaagtgtgcg aacaaacaac taaaaatgca gagaaattgt
    10141 ttaatttaaa ttcataaagt taaaagtgag aaagatcacc gccataaatg taaacgatgc
    10201 tatattcgtt taatatgcta tggttctttc tcactttttt aaattaaaat atcgtacatg
    10261 tgcaatacgt acgatagaga tggttagagc tttgaaatta agaattgtag gaaggcgttt
    10321 taaatgaaaa tcaatgagtt tatagttgta gaaggacgag atgatactga gcgtgttaaa
    10381 cgagctgttg aatgtgatac gattgaaacg aatggtagtg ccatcaacga acaaacttta
    10441 gaagtaatta gaaatgctca acaaagtcga ggcgttattg tattaacaga tccagatttc
    10501 ccaggagata aaattagaag tacaattact gaacatgtca aaggtgttaa acatgcgtat
    10561 attgatagag aaaaagctaa aaataaaaaa gggaaaattg gtgttgaaca tgccgactta
    10621 attgatatta aagaagcgtt aatgcatgtt agttcaccct ttgatgaagc ttatgaatca
    10681 attgataaat ctgtgctaat agagttgggg ttaatcgttg ggaaagatgc aaggcgccgt
    10741 agagaaattt taagtagaaa attgcgaatc ggccattcca atggtaagca gttattgaaa
    10801 aagttaaatg catttggtta taccgaagcg gatgtaaggc aagctttaga agatgaatga
    10861 ggaagtgaaa atgttggata ataaagatat tgcaacacca tcaagaacgc gagcgttgtt
    10921 agataaatat ggctttaatt ttaaaaaaag tttaggacag aactttttga tagatgtgaa
    10981 tatcattaat aatatcattg atgcaagtga tattgatgca caaactgggg tgattgaaat
    11041 tggtccaggt atggggtcgt tgacagaaca attggccaga catgctaaaa gagtattggc
    11101 atttgaaatt gatcaacgtt taatacctgt attaaatgat actctatcac cttatgataa
    11161 tgtgacggtg attaatgaag atattttaaa agcgaatatt aaagaagctg ttgaaaatca
    11221 tttacaagat tgcgaaaaaa taatggttgt tgcaaacctg ccgtactata ttacgacgcc
    11281 aattttatta aatttaatgc aacaagatat accaattgat ggctacgtgg tgatgatgca
    11341 aaaagaagtg ggcgaacgct taaatgctga agtaggttca aaagcatatg gttcattatc
    11401 aattgtcgta caatactata cagagactag taaagtatta acggtaccta aatctgtatt
    11461 tatgccacca cctaatgttg attcaatagt tgtaaaactg atgcagagaa ctgaaccgtt
    11521 agtaacagta gataacgagg aagcattctt taagttagca aaagcagcat ttgcacaaag
    11581 aagaaagaca attaacaata actatcaaaa ttattttaaa gatggtaaac aacacaaaga
    11641 agtgatttta caatggttgg aacaagcagg tattgatcca agacgtcgcg gtgaaacgct
    11701 atctattcaa gattttgcta aattgtatga agaaaagaaa aaattccctc aattagaaaa
    11761 ttaaatgatt gacaaagcaa agcactattg ttaaaattta aattttgttt gacgaaaacg
    11821 ctgcaaatat ggtattatgt aacttgtagc gaggtggagc aatatgccaa aatcaatttt
    11881 ggacatcaaa aattctattg attgtcatgt aggaaatcgt attgtactga aagccaatgg
    11941 aggccgtaag aaaacaataa aacgttctgg aattttaaaa gaaacatatc cgtcagtttt
    12001 cattgttgag ttagatcaag acaaacacaa ctttgagaga gtatcttata catacactga
    12061 tgtgttaact gaaaatgttc aagtttcatt tgaagaggat aatcatcacg aatcaattgc
    12121 acactaaata agacatatag agatgttagg cgtttcttag tataagaagt aaatattatg
    12181 ataattattt gagtgttggg cattatgttc aatactcttt ttatttacaa aatgtttaac
    12241 actgatgttt cgcttataga tttttcagta aatggataat tgtatttata aacacaaata
    12301 caagtaaata ctaagtaatt agatggagaa aattactttt ttattaaaaa aacactaaaa
    12361 aacaaattaa aatgtcaaat attaattctc tttatgttaa aatcatcata ttaagataac
    12421 gaaaagaggg cggaaaatga tatatgaaac ggcaccagcc aaaattaatt ttacgctcga
    12481 tacacttttt aaaagaaatg atggctatca tgagattgaa atgataatga caacagttga
    12541 tttaaatgat cgtttaactt ttcataaaag aaaagatcga aagatagttg ttgagattga
    12601 acataattat gtgccttcta atcataaaaa tctcgcatat cgtgcagcgc aactatttat
    12661 tgagcaatat caactaaagc aaggtgtaac aatttctatc gataaagaaa tacctgtttc
    12721 tgctggctta gctggaggtt cggctgatgc agcagcaacg ttaagaggat tgaatcgact
    12781 ttttgatata ggggcgagtt tggaagaatt ggctctacta ggcagtaaaa ttgggacaga
    12841 tattccgttt tgtatttata ataaaactgc actatgtact ggaagaggag agaaaatcga
    12901 gtttttaaat aaaccacctt cagcttgggt gattcttgct aaaccaaact taggcatatc
    12961 atcaccagat atatttaagt tgatcaattt agataagcgt tacgacgtac atacgaaaat
    13021 gtgttatgag gccttagaaa atcgagatta tcaacaatta tgtcaaagtt tgtctaatcg
    13081 attagagcca atttctgttt caaaacaccc acaaatcgat aaattaaaaa ataatatgtt
    13141 gaaaagtggt gcagatggtg cgttaatgag tggaagcgga ccgactgtgt atgggctagc
    13201 acgaaaagaa agccaagcaa aaaatattta taatgcagtt aacggttgtt gtaatgaagt
    13261 gtacttagtt agactattag gatagaaggg ttgaaaagat gagatataaa cgaagcgaga
    13321 gaattgtttt tatgacgcaa tatttgatga accatccgaa taaattgatt ccattaactt
    13381 tttttgtgaa aaaatttaaa caggcgaaat cttcaataag tgaagatgtc caaattataa
    13441 aaaatacatt ccaaaaagaa aagttaggta cagtaattac tactgctggc gcaagtggtg
    13501 gtgttacgta taaaccaatg atgagtaaag aagaggcgac tgaagttgtt aatgaggtca
    13561 ttactctatt agaagagaaa gaacgtttgt tacctggcgg gtatttattt ttatcagatt
    13621 tggtaggtaa tccatcgcta ctaaacaaag ttggtaagtt aattgccagt atttacatgg
    13681 aagaaaaatt agatgctgtt gttaccattg cgacaaaagg tatttcattg gcaaatgcgg
    13741 ttgctaatat tttaaattta ccagtagtag tgattagaaa agacaacaag gtgactgaag
    13801 gttctacagt ttcaattaat tacgtttcag gatcttcaag aaaaatagag acaatggtac
    13861 tttcgaagag aactttagca gaaaattcaa atgttttagt tgtcgatgat tttatgaggg
    13921 ctggtggctc tattaatggt gttatgaatt taatgaatga gtttaaagcc catgtaaaag
    13981 gggtatcagt acttgtagaa tcaaaagaag ttaaacaaag attgattgaa gattatactt
    14041 ccttagtgaa attatctgat gtagatgaat ataatcaaga gtttaacgta gaacctggca
    14101 acagtttatc taagttttca taaaaggagt tttagtatta tgaaaatcat taacacaaca
    14161 agattaccgg aagcacttgg accatattcg catgcaacag ttgtgaatgg tatggtttat
    14221 acttctggtc agattccatt gaatgttgat gggaaaatcg taagcgctga tgttcaagca
    14281 cagacaaaac aagttttaga aaatttaaag gttgttttgg aagaagcagg atctgatttg
    14341 aattctgttg cgaaagcgac cattttcatt aaagatatga atgatttcca aaaaataaat
    14401 gaagtgtatg gtcaatattt taatgaacac aagccagcgc gtagttgtgt agaggttgcg
    14461 cgtttgccaa aagatgtgaa agtagaaatt gaattagtaa gtaaaattaa ggaattataa
    14521 ttttcgatta atatgtttaa tcaagcttct aaataaaaca gagagatata tactataggg
    14581 gggctcacta catgaaagtg acagatgtaa gacttagaaa aatacaaaca gatggacgaa
    14641 tgaaagcact cgtttccatt acattagatg aagctttcgt aattcatgat ttacgtgtaa
    14701 ttgaaggaaa ctctggcttg ttcgttgcaa tgccaagtaa acgtacacca gatggtgaat
    14761 tccgcgacat cgcgcatcct attaattcag atatgagaca agaaattcaa gatgcagtga
    14821 tgaaagtata tgatgaaaca gatgaagtag taccagataa aaacgctaca tcagaagatt
    14881 cagaagaagc ttaatcaatt ttatatttag cgatgtaata catttgcaat aagttgattt
    14941 gatactgtcg ataaagcata aagctttgtc ggcagttttt ttagtttgta ttaatgtttt
    15001 tttagtttgt attaatgttt ttttattttt aatgaaaggc taataaatat atacgttaac
    15061 agattatgat gatatgaaaa ttattgattc attgctgata aaaattgcgt ttgaatgatg
    15121 ctcgtatttt tgaagtaaga aaaaagttgt ttttaaaatt acaacgaatt aaaaacaatg
    15181 ccttttatat gttgaaagag tattgcagat taaattataa taatgacgaa gtgtaaaatt
    15241 taatgggggt taatgttcat gcgaagacac gcgataattt tggcagcagg taaaggcaca
    15301 agaatgaaat ctaaaaagta taaagtgcta cacgaggttg ctgggaaacc tatggtcgaa
    15361 catgtattgg aaagtgtgaa aggctctggt gtcgatcaag ttgtaaccat cgtaggacat
    15421 ggtgctgaaa gtgtaaaagg acatttaggc gagcgttctt tatacagttt tcaagagaaa
    15481 caactcggta ctgcgcatgc agtgcaaatg gcgaaatcac acttagaaga caaggaaggt
    15541 acgacaatcg ttgtatgtgg tgacacaccg ctcatcacaa aggaaacatt agaaacattg
    15601 attgcgcatc atgaggatgc taatgctcaa gcaactgtat tatctgcatc gattcaacaa
    15661 ccatatggat acggaagaat cgttcgaaat gcgtcaggtc gtttagaacg catagttgaa
    15721 gagaaagatg caacgcaagc tgaaaaggat attaatgaaa ttagttcagg tatttttgcg
    15781 tttaataata aaacgttgtt tgaaaaatta acacaagtga aaaatgataa tgcgcaaggt
    15841 gaatattacc tccctgatgt attgtcgtta attttaaatg atggcggcat cgtagaagtg
    15901 tatcgtacca atgatgttga agaaatcatg ggtgtaaatg atcgtgtaat gcttagtcag
    15961 gctgagaagg cgatgcaacg tcgtacgaat cattatcaca tgctaaatgg tgtgacaatc
    16021 atcgatcctg acagcacttt tattggtcca gacgttacaa ttggtagtga tacagtcatt
    16081 gaaccaggcg tacgaattaa tggtcgtaca gaaattggcg aagatgttgt tattggtcag
    16141 tactctgaaa ttaacaatag tacgattgaa aatggtgcat gtattcaaca gtctgttgtt
    16201 aatgatgcta gcgtaggagc taatactaag gtcggaccgt ttgcgcaatt gagaccaggc
    16261 gcgcaattag gtgcagatgt taaggttgga aattttgtag aaattaaaaa agcagatctt
    16321 aaagatggtg ccaaggtttc acatttaagt tatattggcg atgctgtaat tggcgaacgt
    16381 actaatattg gttgcggaac gattacagtt aactatgatg gtgaaaataa atttaaaact
    16441 atcgtcggca aagattcatt tgtaggttgc aatgttaatt tagtagcacc tgtaacaatt
    16501 ggtgatgatg tattggtggc agctggttcc acaatcacag atgacgtacc aaatgacagt
    16561 ttagctgtgg caagagcaag acaaacaaca aaagaaggat ataggaaata atcatttacg
    16621 tatttaaaat ggctaggata aaaggataat cctatgtaat attaatgtaa tctttatgat
    16681 ttaatgattc gcatagtaat ggagttacat tttatatata atagtaattg cgtaagtaaa
    16741 taattggagg actataaatg ttaaataatg aatataagaa ttcgtcatta aagatttttt
    16801 cattgaaagg aaacgaagca ttagcgcaag aagttgctga ccaagtagga attgaactag
    16861 gtaaatgttc agttaaacgt tttagtgatg gagaaattca aattaatatc gaagagagta
    16921 ttcgtggttg tgacgtattt attattcaac caacatcata tcctgtgaat ctacatttaa
    16981 tggaattatt aattatgatt gatgcttgta aacgtgcttc tgcagcaaca atcaatattg
    17041 tagtgccata ttatggatat gcaagacaag atagaaaagc ccgtagccgt gagccaatca
    17101 ctgctaaatt agttgcaaac ttaatcgaaa cagctggcgc aactcgtatg attgcgttag
    17161 acttacatgc accacaaatt caaggattct ttgatattcc aattgaccac ttaatgggtg
    17221 tgccaattct tgctaaacat ttcaaagatg atccgaatat taacccagaa gaatgtgtcg
    17281 ttgtttcacc agaccatggc ggtgttacac gtgcacgtaa attagctgac attttaaaaa
    17341 ctccaattgc aattatagat aaacgtcgtc ctagaccaaa tgttgctgaa gtgatgaaca
    17401 ttgttggtga gattgaagga cgtacggcaa ttattattga cgatattatt gatacagcag
    17461 gtacaatcac tttagctgca caagcattaa aagataaagg tgctaaagaa gtatatgctt
    17521 gttgtacaca ccctgtttta tcaggaccgg ctaaagaacg tatcgaaaat tctgctataa
    17581 aagaattaat cgtaacaaac tcaattcatt tagatgaaga tcgcaaacca tctaacacta
    17641 aagaattatc tgttgctggt ttaatcgcac aagctatcat tcgtgtatac gaaagagaat
    17701 cagttagcgt attatttgac taatatttaa aaggcgtttg acgaacatat tccaaacgtg
    17761 tataatagtt tcgttcgtga ttatacgaat aaataaacac ttgcaagcaa cgatgatgtt
    17821 gatgggtaag tgaggtgctc gttttgagca aaaatgaaag gtggaaatga gaatggcttc
    17881 attaaagtca atcatccgtc aaggtaaaca aacacgttca gatcttaaac aattaagaaa
    17941 atctggtaaa gtaccagcag tagtatacgg ttacggtact aaaaacgtgt cagttaaagt
    18001 tgatgaagta gaattcatca aagttatccg tgaagtaggt cgtaacggtg ttatcgaatt
    18061 aggcgttggt tctaaaacta tcaaagttat ggttgcagac taccaattcg atccacttaa
    18121 aaaccaaatt actcacattg acttcttagc aatcaatatg agtgaagaac gtactgttga
    18181 agtaccagtt caattagttg gtgaagcagt aggcgctaaa gaaggcggcg tagttgaaca
    18241 accattattc aacttagaag taactgctac tccagacaat attccagaag caatcgaagt
    18301 agacattact gaattaaaca ttaacgacag tttaactgtt gctgatgtta aagtaactgg
    18361 cgacttcaaa atcgaaaacg attcagctga atcagttgta acagtagttg ctccaactga
    18421 agaaccaact gaagaagaaa tcgaagctat ggaaggcgaa caacaaactg aagaaccaga
    18481 agttgttggc gaaagcaaag aagacgaaga aaaaactgaa gagtaatttt aatctgttac
    18541 attaaagttt ttatactttg tttaacaagc actgtgctta ttttaatata agcatggtgc
    18601 tttttgtgtt attataaagc ttaattaaac tttattactt tgtactaaag tttaattaat
    18661 tttagtgagt aaaagatatt aaactcaaca atgatacatc ataaaaattt taatgtactc
    18721 gattttaaaa tacatactta ctaagctaaa gaataatgat aattgatggc aatggcggaa
    18781 aatagatgtt gtcattataa taataaatga aacaattatg ttggaggtaa acacgcatga
    18841 aatgtattgt aggtctaggt aatataggta aacgttttga acttacaaga cataatatcg
    18901 gctttgaagt cgttgattat attttagaga aaaataattt ttcattagat aaacaaaagt
    18961 ttaaaggtgc atatacaatt gaacgaatga acggagataa agtgttattt atcgaaccaa
    19021 tgacaatgat gaatttgtca ggagaagcag ttgcaccgat tatggattat tacaatgtta
    19081 atccagaaga tttaattgtc ttatatgatg atttagattt agaacaagga caagttcgct
    19141 taagacaaaa aggaagtgcg ggcggtcaca atggtatgaa atcaattatt aaaatgcttg
    19201 gtacagacca atttaaacgt attcgtattg gtgtgggaag accaacgaat ggtatgacgg
    19261 tacctgatta tgttttacaa cgcttttcaa atgatgaaat ggtaacgatg gaaaaagtta
    19321 tcgaacacgc agcacgcgca attgaaaagt ttgttgaaac atcacgattt gaccatgtta
    19381 tgaatgaatt taatggtgaa gtgaaataat gacaatattg acaacgctta taaaagaaga
    19441 taatcatttt caagacctta atcaggtatt tggacaagca aacacactag taactggtct
    19501 ttccccgtca gctaaagtga cgatgattgc tgaaaaatat gcacaaagta atcaacagtt
    19561 attattaatt accaataatt tataccaagc agataaatta gaaacagatt tacttcaatt
    19621 tatagatgct gaagaattgt ataagtatcc tgtgcaagat attatgaccg aagagttttc
    19681 aacacaaagc cctcaactga tgagtgaacg tattagaact ttaactgcgt tagctcaagg
    19741 taagaaaggg ttatttatcg ttcctttaaa tggtttgaaa aagtggttaa ctcctgttga
    19801 aatgtggcaa aatcaccaaa tgacattgcg tgttggtgag gatatcgatg tggaccaatt
    19861 tcttaacaaa ttagttaata tggggtacaa acgggaatcc gtggtatcgc atattggtga
    19921 attctcattg cgaggaggta ttatcgatat ctttccgcta attggggaac caatcagaat
    19981 tgagctattt gataccgaaa ttgattctat tcgcgatttt gatgttgaaa cgcagcgttc
    20041 caaagataat attgaagaag tcgatatcac aactgcaagt gattatatca ttactgaaga
    20101 agtgattaga catcttaaag aagagttgaa aactgcatat gaaaatacaa gacccaaaat
    20161 agataaatca gtgcgcaatg atctgaaaga aacgtatgaa agctttaaat tattcgaaag
    20221 tacatacttt gatcatcaaa tactacgtcg tttagtagcg tttatgtatg aaacaccttc
    20281 gacaattatt gagtatttcc aaaaagatgc aatcattgca gttgatgaat ttaatcgtat
    20341 taaagaaact gaagaaagtt taacagtaga gtctgattcg tttattagca atattattga
    20401 aagtggtaat ggatttatag gacaaagttt tataaaatat gatgattttg aaacattgat
    20461 tgaaggctat cctgtcactt atttttcatt attcgctaca acaatgccga taaaactaaa
    20521 tcatattatt aaattttcat gtaaacctgt ccaacaattt tatgggcaat atgacattat
    20581 gcgttctgaa tttcaacgat atgtcaatca aaattatcat atcgtggttt tggtcgaaac
    20641 tgaaactaaa gttgaacgta tgcaagcgat gttaagtgaa atgcatattc catcaataac
    20701 aaaattgcat cgctcaatgt catcggggca agcagtgatt attgaaggca gtttatctga
    20761 aggatttgaa ctacctgata tgggattagt tgtcattact gagcgtgagc tttttaaatc
    20821 aaaacagaaa aagcaacgaa aacgtacgaa agctatctca aatgctgaaa aaattaagtc
    20881 ttaccaagat ttaaatgtgg gagattatat tgttcatgtg catcatggtg ttggtagata
    20941 tttaggtgtt gagacgctcg aagtggggca aacgcatcgt gattatatta aattgcaata
    21001 taaaggtacg gatcaactat ttgttccagt agatcaaatg gatcaagttc aaaaatatgt
    21061 agcttcggaa gataagacgc caaaattaaa taaactcggt ggcagtgaat ggaaaaaaac
    21121 aaaagcaaaa gttcaacaaa gtgttgaaga tattgctgaa gagttgattg atttatataa
    21181 agaaagagaa atggcagaag gttatcaata tggggaagac acagctgagc aaacaacatt
    21241 tgaattagat tttccatatg aacttacgcc tgaccaagct aaatctatcg atgaaattaa
    21301 agatgacatg caaaaatcgc gtccaatgga tcgcttgcta tgtggtgatg ttggttatgg
    21361 taaaactgaa gttgcagtga gagcagcatt caaagctgta atggaaggaa agcaagttgc
    21421 atttttagtt cctacaacta ttttagctca gcaacattat gagacgttaa ttgagcgtat
    21481 gcaagatttt cctgttgaaa ttcaattaat gagtcgtttt agaacgccga aagagataaa
    21541 acaaactaag gaaggactta aaactgggtt tgttgacata gttgttggta cacacaaatt
    21601 acttagtaaa gatatacagt ataaagattt agggctgttg attgtagatg aagaacaacg
    21661 atttggtgta cgccataaag agcgtattaa aacattaaaa cataatgtag atgtactaac
    21721 attgactgca accccaatac ctagaacatt gcatatgagt atgctaggtg tgcgcgattt
    21781 gtcagtgatt gaaacgccgc cagaaaatcg tttcccagtt caaacatatg tattagaaca
    21841 gaacatgagt tttatcaaag aagctttaga aagagaacta tcccgtgatg gccaagtgtt
    21901 ttatctttat aataaagtgc aatccattta tgaaaaacga gaacaactcc agatgttaat
    21961 gccagatgct aacattgcag ttgctcatgg acaaatgaca gagcgcgatt tagaagaaac
    22021 gatgttaagt tttatcaata atgaatatga tattttagta acgacgacga ttattgaaac
    22081 aggtgtcgat gtcccaaatg caaatacttt gatcattgaa gatgcagatc gctttggatt
    22141 gagtcagttg tatcaattaa gaggtcgtgt tggtcgttca agtcgtattg gttatgcata
    22201 cttcttacat ccagcaaata aggtactaac tgagactgca gaagatcgat tacaagcgat
    22261 taaagaattt acggagttag gctcaggatt taagattgcg atgcgtgatt tgaacattcg
    22321 tggtgctggt aatttgttag gtaaacaaca gcacggcttt attgatacag ttggatttga
    22381 tttgtacagt caaatgttag aagaagctgt aaatgaaaaa cgtggtatta aggaaccaga
    22441 atctgaggtg ccagaagtcg aagttgattt aaacttggat gcatatttgc caacagaata
    22501 tattgcaaat gaacaagcta aaattgaaat ttataaaaag ctacgaaaaa ctgaaacatt
    22561 tgatcaaatt atcgacatta aagatgaatt aattgatcgt tttaatgatt atcctgttga
    22621 agtagcacgt ttgcttgata tagtggaaat aaaagtacac gcattacatt caggtatcac
    22681 gttgattaaa gataaaggga aaataattga tattcattta tctgtaaaag ccactgaaaa
    22741 tattgatggc gaagtgctgt tcaaagcaac acaaccttta ggtagaacaa tgaaggttgg
    22801 tgttcaaaat aatgcaatga caattacttt aacgaaacaa aatcaatggc ttgatagttt
    22861 gaagttttta gttaagtgca ttgaagaaag catgagaatc agtgatgaag cataaagaag
    22921 catttaatgg cgttgtcgtg ttaactgctg cattaattgt cattaaaatt ctgagtgctg
    22981 tatatcgaat tccatatcaa aatatattag gtgatacagg tttgtatgca tatcaacaag
    23041 tgtatccaat tgtagcatta ggaatgatat tatcgatgaa tgccattcct agtgcaatta
    23101 cacaaaatat agggaagtat catagtgacg aagcatatgc aaaagcggtc gcttatatac
    23161 aattagttgg tatattatta tttattgcta tttttgtgtt tgcgaacaat attgcacata
    23221 tgatgggtga tagccattta acaccaatga ttcaagcagc aagtttaagc tttatattta
    23281 taggtatgct tggcgtgtta agaggttatt atcaatctgc aaataatatg acagttccgg
    23341 ctatttccca ggttatagaa caagttatac gagtaggtat tatcattgtt actattgtta
    23401 tttttgtaga cagaggttgg acgatatatg aagcgggaac aattgctatt ttagcatcaa
    23461 cgataggttt tttaggttct tcaatttatt tagtagcgca ccgacctttt aagtttaaaa
    23521 tggtaaataa cactgcaaag attgtttgga aacagttcgc actttcggtt ttgattttcg
    23581 ctatcagtca attaatcgta attttatggc aagtgattga tagtgttact attattaagt
    23641 cacttcaagc gatacgcgtg ccattcgatg ttgccataac tgaaaaagga gtctatgacc
    23701 gtggtgcatc atttattcag atgggattga ttgtaactac aacatttagt tttgcgctca
    23761 ttcctctgtt aagtgacgca atcaaaatga ataatcaggt acttatgaat cgttatgcaa
    23821 atgcgtcatt aaagattacg attttaataa gtacagcagc gggaatagga ttaattaatt
    23881 tattgccttt aatgaacggt gtgtttttta agacgaatga tttaacctta acgttaagtg
    23941 tttatatgat tacggtcatt tgtgtatcgt taattatgat ggatatggct ttattacaag
    24001 cgcaacatgc tgtgagacct atttttgttg gtatgacggc aggattggtt attaaattta
    24061 tacttaatat cattttgatt cgtttaagtg gcattattgg tgcgagcatt agtactgttg
    24121 tatcattaat tatattcggt acgattatcc atattgctgt cacgagaaaa taccacttac
    24181 atgcgatgag acgatttttt atcaatgttg ttttaggtat ggtatttatg tcgattgttg
    24241 ttcaatgcgt gttaaacata gtgacaacac acggtagatt cactggactc attgaattat
    24301 tatgtgcagc agtattaggt atcattgcat tgtttttcta tatttttaga tttaatgttt
    24361 tgacatataa agagttaact tatttaccat ttggttcaaa gttgtatcaa attaagaaag
    24421 gaagacgttg atggcacata ccattacgat tgttggctta ggaaactatg gcattgatga
    24481 tttgccgcta gggatatata aatttttaaa gacacaagat aaagtttatg caagaacgtt
    24541 agatcatcca gttatagaat cattgcaaga tgaattaaca tttcagagtt ttgaccatgt
    24601 ttatgaagca catgaccaat ttgaagatgt ctataacgat attgtggcgc aattggttga
    24661 agctgctaat gaaaaagata ttgtctatgc ggttccgggt catcctagag ttgctgagac
    24721 aactacagtg aaattactgg ctttagcaaa ggacaatact gatatagatg tgaaagtttt
    24781 aggtggtaaa agctttattg atgatgtgtt tgaagcagtt aatgtagatc caaatgatgg
    24841 cttcacactg ttagatgcga catcattaca agaagtaata cttaatgtta gaacgcatac
    24901 attgattacg caagtttata gtgcaatggt tgctgctaat ttgaaaatca ctttaatgga
    24961 acgatatcct gatgattacc ctgttcaaat tgtcactggt gcacgaagcg atggtgcgga
    25021 taacgttgtg acatgcccat tatatgaatt ggatcatgat gaaaatgcat tcaataattt
    25081 gacgagtgta ttcgtaccaa aaatcataac atcgacatat ttgtatcatg actttgattt
    25141 tgcaacggaa gtgattgata ctttagttga tgaagataaa ggttgtccat gggataaagt
    25201 gcaaacgcat gaaacgctta agcgttattt acttgaagaa acatttgaat tgttcgaagc
    25261 tattgacaat gaagatgatt ggcatatgat tgaagaacta ggagatattt tattacaagt
    25321 gttattgcat actagtattg gtaaaaaaga agggtatatc gacattaaag aagtgattac
    25381 aagtcttaat gctaaaatga ttcgtagaca cccacacata tttggtgatg ccaatgctga
    25441 aactatcgat gacttaaaag aaatttggtc taaggcgaaa gatgctgaag gtaaacagcc
    25501 aagagttaaa tttgaaaaag tatttgcaga gcatttttta aatttatatg agaagacgaa
    25561 ggataagtca tttgatgagg cctcgttaaa gcagtggcta gaaaaagggg agagtaatac
    25621 atgagattag ataaatattt aaaagtatca cggttaataa agcgacgtac gctagcaaaa
    25681 gaagtaagtg atcaaggtag aattacaata aatggtaatg ttgctaaagc tggatcggat
    25741 gttaaagttg aagatgtgct gacgattcgc tttggtcaaa aattagtaac agttaaagta
    25801 actgcattaa atgaacatgc atctaaagat aacgcgaagg gcatgtatga aatcattgaa
    25861 gagcgtcgac ttgaagaagc gtaaattgga ggtgacaagc aatgaaaaat aaagtagaac
    25921 atatagaaaa tcagtacacg tcgcaagaga acaagaaaaa acaacgtcaa aaaatgaaaa
    25981 tgcgtgttgt tcgtaggcgt attacagtat ttgcgggcgt attacttgcg ataattgttg
    26041 ttttatcaat cttgcttgtt gtccaaaaac atcgcaatga tattgatgca caggagcgaa
    26101 aagcgaaaga agcacagttt caaaagcaac aaaatgaaga aattgcgtta aaagaaaagt
    26161 tgaataatct gaatgacaaa gattacattg aaaaaattgc gcgtgatgat tattacttaa
    26221 gcaacaaagg tgaagtgatt tttaggttgc cagaagacaa agattcgtct agctcaaaat
    26281 cttcgaaaaa ataaatccaa attgattcaa aattatccga gtatagacat tgtgaaaaaa
    26341 tccaaacaag gatataataa gggaaaatcg aatcaaatcg ggaggattta tttaacatat
    26401 gtcaatcgaa gttggaaata agcttaaagg taaagtcact ggtattaaaa agtttggtgc
    26461 attcgtagaa ttacctgaag gaaaaagtgg tttagttcac attagtgaag tcgcagataa
    26521 ttatgttgaa aacgtagaag agcacctttc tgttggtgat gaagtagacg tgaaagtatt
    26581 atctattgct gatgatggaa aaattagtct ttcaattaag aaagctaaag accgtccacg
    26641 tagacaacat acgagtaaac caagtcatca aaaaccagtg caaaaagccg aagattttga
    26701 aaagaaatta agcaatttct taaaagatag tgaagataaa ttaacttcaa tcaaacgtca
    26761 aacagaatct agacgcggtg gcaaaggttc tagacgttaa ttaaaataaa taaagactgt
    26821 ttcgataagg aatatattta gaatgatgcg tatcgaataa tcgattgcag cgttagacaa
    26881 tctaagactg tttcttaaat aaggagcagt ctcttttatt tgtaatgata taactaagac
    26941 ttataccatt tttgaaaatt gtaaaagtga ggtgatgtta tgcagttaaa tagtaatggt
    27001 tggcatgttg atgaccatat tgttgtcgct gtttctacag gtattgatag tatgtgttta
    27061 ttgtatcaac tactaaatga ttataaagat agttatagaa aactaacatg cttacatgtc
    27121 aatcatggcg ttaggtcagc ttcaattgag gaagccagat ttttagaagc atactgcgaa
    27181 cgtcatcaca ttgatttaca tatcaaaaag ttagatttgt cgcatagtct cgaccgaaat
    27241 aacagcattc agaatgaagc tcgaattaaa cgttacgaat ggtttgatga aatgatgaat
    27301 gtattagaag cggatgtatt gctaacggcg catcatttgg acgatcaatt agaaactatt
    27361 atgtatcgta tttttaatgg gaaatcaacg cgtaataaac taggatttga tgagttatcg
    27421 aagcgaaaag gttatcagat ttatcgacca cttttagctg tctctaaaaa agaaataaaa
    27481 caattccaag agagatatca tattccatat tttgaagatg aatctaataa agataacaaa
    27541 tatattagaa atgatattcg taatagaatt attccagcta ttgatgaaaa taatcaactt
    27601 aaagtatcgc atttattaaa attaaaacaa tggcatgatg aacaatatga tattttgcaa
    27661 tattcagcta aacaatttat tcaagaattt gtgaagtttg atgaacagtc aaaatattta
    27721 gaggtttcta gacaagcttt taataactta ccaaactcat taaagatggt tgtgttggac
    27781 tgcctattat caaagtatta tgagttgttt aatattagtg ctaaaacata cgaagagtgg
    27841 tttaaacaat ttagtagtaa gaaagcacaa ttcagtatta atctcacgga taaatggata
    27901 attcaaatcg catatggtaa attaataata atggctaaaa ataatggcga tacatatttt
    27961 agagttcaaa caattaaaaa gccaggtaat tattttttta acaaatatcg attagagata
    28021 cattctaatt taccaaaatg tttatttccg cttacagtga gaacacgaca aagtggcgat
    28081 acatttaaac tgaatgggcg cgatggttat aagaaagtga atcgcctgtt tatagattgt
    28141 aaagtgccac agtgggttcg ggatcaaatg ccaatcgtat tggataaaca acagcgcatt
    28201 attgcggtag gagatttata tcaacaacaa acaataaaaa aatggattat aattagtaaa
    28261 aatggagatg aatagcgtta tgcataatga tttgaaagaa gtattgttaa ctgaagaaga
    28321 tattcaaaat atctgtaagg aattgggagc acaattaaca aaggattatc aaggtaaacc
    28381 attagtatgc gtgggtatct taaaaggctc agcaatgttt atgtcagatt taattaaacg
    28441 aattgatacc catttatcaa ttgatttcat ggatgtttct agttatcacg gaggcactga
    28501 gtcaactggt gaagttcaaa tcattaaaga tttaggttct tctattgaaa ataaagacgt
    28561 attaattatt gaagatatct tagagactgg tactacactt aagtcaatta ctgaattatt
    28621 acaatctaga aaagttaatt cattagaaat agttacttta ttagataaac caaaccgtcg
    28681 taaagcggac attgaagcta agtatgtagg taaaaaaata ccagatgaat ttgttgttgg
    28741 ttacggttta gattatcgtg aattataccg aaacttacca tatatcggta cgttaaaacc
    28801 tgaagtgtat tcaaattaat tttttaatca atttcagtta ttattactat gcgtttgaga
    28861 aataatagtg tagactcaaa aatatgaaaa atgtatttca tatatattta attttagaca
    28921 agacatatgt cttgaaaagt tgaaaaatat agagattgat aaaactaata cgggtgtgaa
    28981 tgacattgat gttaagctca attactagct tataaaacat gtcatatgtt acaatttttg
    29041 ttagttttat tatgggaagt aggaggaaat gacgcatgca gaaagctttt cgcaatgtgc
    29101 tagttatcgt aataataggc gttattattt ttggtctatt ttcatattta aacggtaatg
    29161 gaaatatgcc gaaacagctt acatataatc aatttactga gaagttggaa aaaggtgacc
    29221 ttaaaacttt agaaatccaa ccacaacaaa atgtctatat ggtaagtggt aaaacgaaaa
    29281 atgatgaaga ctattcatca actattttat ataacaacga aaaagaatta caaaaaatta
    29341 ctgatgctgc taaaaagcaa aacggtgtaa aattaacgat taaagaagaa gaaaaacaaa
    29401 gtgtctttgt gagtatactt tcaacattaa ttccagttgt agtcatagcg ttattattta
    29461 ttttcttcct aagccaagca caaggtggcg gtagtggcgg tcgtatgatg aactttggta
    29521 aatctaaagc aaaaatgtac gataataata aacgtcgtgt tcgtttctct gatgtagcag
    29581 gggcagatga agaaaaacaa gaattaattg aaattgttga tttcttgaaa gataataaaa
    29641 aattcaaaga aatgggatct aggattccta aaggtgtctt acttgttgga cctccaggta
    29701 ctggtaaaac attacttgct agagcggttg caggtgaagc tggcgcacca ttcttctcta
    29761 ttagtggttc agactttgta gagatgtttg ttggtgttgg tgcgagccgt gttcgtgact
    29821 tattcgataa tgctaagaaa aacgcgcctt gtatcatctt tatcgatgag attgatgctg
    29881 ttggtcgtca acgtggtgca ggtgttggtg gcggtcatga tgaacgtgaa caaaccctaa
    29941 accaattatt agttgaaatg gatggtttcg gtgaaaatga aggtatcatt atgatagctg
    30001 ctacaaaccg tcctgatatc cttgacccag ccttattacg tccaggtcgt tttgatagac
    30061 aaattcaagt tggtcgtcca gatgtgaaag gccgtgaagc aattcttcat gttcatgcta
    30121 aaaacaaacc acttgatgaa acggttgatt taaaagcaat ttcacaacgt acacctggtt
    30181 tctcaggtgc tgatttagag aacttattaa atgaagcatc tttaattgct gtacgtgaag
    30241 gtaaaaagaa aattgacatg agagatatcg aagaggcaac ggatagagtt atagccggac
    30301 ctgctaagaa atctcgagtt atttctaaga aagaacgtaa tattgttgct catcacgaag
    30361 ctggtcatac aattatcggt atggtacttg atgaggcaga agtagtgcat aaagttacta
    30421 ttgttccacg tggacaagca ggtggttatg caatgatgct acctaaacaa gatcgtttct
    30481 taatgactga acaagagtta ttagataaaa tctgtggttt acttggtgga cgtgtatcag
    30541 aagatattaa ctttaacgaa gtatcaacag gtgcttcaaa tgacttcgaa cgtgcaacac
    30601 aaatcgcacg ctcaatggtt acgcaatatg gtatgagtaa aaaattagga ccattacagt
    30661 tcggtcatag caatggtcaa gtattcttag gtaaagatat gcaaggtgag cctaattatt
    30721 caagccaaat cgcatatgaa attgataaag aagttcaacg aatcgttaaa gaacaatacg
    30781 aacgttgtaa acaaatttta ttagagcaca aagaacaatt aattttaatt gctgaaacat
    30841 tattaacaga agaaacatta gttgctgaac aaattcaatc attattctac gaaggtaaat
    30901 tacctgaaat tgattatgat gcagctaaag ttgttaaaga tgaagattct gaatttaatg
    30961 atggtaaatt cggtaaatct tatgaagaga ttcgtaaaga gcaattagaa gatggacaac
    31021 gtgacgaaag tgaagatcgt aaagaagaaa aagatattgc tgaggataaa aaagaagctg
    31081 ataaatctga tgaaaaagat gaaccagcac atcgacaagc cccaaatatc gaaaaacctt
    31141 acgatccaaa tcacccagac aataaataat cgattatatt cagtacctct ttctatgata
    31201 aagttataga aagaggtact tttatcgttt t
//
LOCUS       DAOBBH010000010        30961 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.010, whole genome shotgun sequence.
ACCESSION   DAOBBH010000010 DAOBBH010000000
VERSION     DAOBBH010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 30961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30961
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.010"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..222)
                     /locus_tag="P6L01_000298"
     CDS             complement(<1..222)
                     /locus_tag="P6L01_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HDP3029399.1"
                     /translation="MLLSFVVTVIVVYVSSFWWMTPFITYITRAHVSLHAFSFTEMIQ
                     IYVMIIFFIAFCFISPVMFYQLWAFIAPGL"
     gene            complement(278..502)
                     /locus_tag="P6L01_000299"
     CDS             complement(278..502)
                     /locus_tag="P6L01_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HDP3029400.1"
                     /translation="MIIMITNTFILGITGPTSLVVISIIALIIFGPKKLPQFGRAIGS
                     TLKEFKSATEDLDKESHDTPSKESKQQREQ"
     gene            complement(602..991)
                     /locus_tag="P6L01_000300"
     CDS             complement(602..991)
                     /locus_tag="P6L01_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HDP3029401.1"
                     /translation="MAFTLSAIQQAHQQFTGVDFPKLFKAFKDMGMTYNIVNIQDGTA
                     TYVHQSEDDIVTSSVKSNHPVAQKSNKTIVQDVLTRHQQGQTDFETFCDEMAEAGIYK
                     WHIDIQAGTCTYIDLKEQAIISELIPQ"
     gene            1231..1434
                     /locus_tag="P6L01_000301"
     CDS             1231..1434
                     /locus_tag="P6L01_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDP3029402.1"
                     /translation="MRNRLKELRARDGLNQTQLAKLAGVSRQTISLIERNNFMPSVLT
                     AIKIARIFNETVETVFIIEEDEA"
     gene            1431..2144
                     /locus_tag="P6L01_000302"
     CDS             1431..2144
                     /locus_tag="P6L01_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HDP3029403.1"
                     /translation="MKILRYIGYLLLGGIVGGIIGGILGNFDGLGIENLTFATYNNVV
                     VISIVATMIIILVEAIVLMNQRRALKYKRLVDEEVDIDATDQYELLANRYVLNGSILS
                     VIQTIIAFVVLLIFVVGQAEANAMLFFLIPFFASAIFNTQFTLFNRKFDDRMPKIADK
                     NYTEKRLEILDEGERHIELIALFKTYAINLSILILAIIFIGSYSIATGINQSFSLLLI
                     IAILIYNAFSYLLKRRRFY"
     gene            2169..3011
                     /locus_tag="P6L01_000303"
     CDS             2169..3011
                     /locus_tag="P6L01_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3029404.1"
                     /translation="MTTLLNVDRVNKQYKDSDFKLQDASLTISTNETVGLIGKNGSGK
                     STLINILVGNRHKDNGSIAFFGEEHAADDVEYKEHIGVVFDDLRVPNKLTIKDIDKVF
                     QSIYTTWNSQKFFDLIKYFELPLQTKIKTFSRGMRMKIALTIALSHDVKLLILDEATA
                     GMDVSGREEVMELLEDFVAQGGGILISSHISEDIEQLADKLVFMKDGRMILTEQKDIL
                     LAQYGIVTTGDKDVEIPKHLIIASRLSKGKYQILVKDYAEIENAEPLKHIDDATKIIM
                     RGEV"
     gene            3011..3640
                     /locus_tag="P6L01_000304"
     CDS             3011..3640
                     /locus_tag="P6L01_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDP3029405.1"
                     /translation="MKGMFLSSFYATRKQTYIYFIVAIIAAGYFAVFNPLMSSAMAGV
                     MLITPITDNIKHEKDSRWMYYVSTLPVKRSDYIKSYFAFYLILFGASLMIGLVVTTIV
                     TQSVMIGIMSGLMSFGIIGAYSIIFPLTFKFGAENSNVIMIVASILLLFSFVVFFFIY
                     GMVSGASALEFEKISTEGWLVVIVYAVIGIVITSVSYILSIKIFNKQEL"
     gene            complement(4027..5160)
                     /locus_tag="P6L01_000305"
     CDS             complement(4027..5160)
                     /locus_tag="P6L01_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HDP3029406.1"
                     /translation="MVNALTPKHLAEKRAGFHELFFDLIFVYAIQKIAHVILTTQNGS
                     ISADLFFKYIVMSLFLWLMWSHQTFFTNRFGQVTFKDVSFMMFNMFIMVFLSNSLYPD
                     FEKTFFPFFLCVAIMYLSIGLQYLLHIRTGLDYGDKRTCQAFASVAFVISFLSFLSLV
                     LPQSIHYIPGFLGVFIAATGLIPFQKYLVLSPVNMMHLVERFSLLTIIIFGEVLVGLA
                     SSSFSIDHFSYIYIFQFMILISLFGVYWIITENYINHKLSSIGFRLSYTHLLINIALG
                     VINAAIVFSNNNKLNDLFEINMMYISVLIFYIGLWLITPYFHNELTNAKYISSSLGIL
                     VVSYIISLIFKGHDQVMIISVSVATFCIMLIYFKNQRLRNKHE"
     gene            5699..6880
                     /locus_tag="P6L01_000306"
     CDS             5699..6880
                     /locus_tag="P6L01_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDP3029407.1"
                     /translation="MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLN
                     PSEIDEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSI
                     VTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGIT
                     AENLVEQYGISREEQDTFAVNSQHKAVRAQQNGEFDSEIVPVSIPQRKGEPILVTKDE
                     GVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVL
                     DGFGSHGVDPSIMGIAPVGAVEKALKRSKKELSDIDVFELNEAFAAQLLAVDRELKLP
                     PEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKY
                     K"
     gene            complement(6963..7715)
                     /locus_tag="P6L01_000307"
     CDS             complement(6963..7715)
                     /locus_tag="P6L01_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDP3029408.1"
                     /translation="MTQYPLWQQLNTLKQATWVDLTHTFDPEIPRFSEFEKGEVSTLF
                     TVKDHGFYVQRWNIVTQYGTHIDAPIHFVENKRYLEDIDLKELVLPLIVLDFSTEVAN
                     NNDFIVTRAHIEAWEKEHGTIEPGTFVALRTDWSKRWPNIEKFENKDANGQQHAPGWG
                     LDALKYLIEERRVEAVGHETFDTDASVDVVKNGDLVGERYILGQDKYQVELLTNLDQL
                     PTRGAIIYAISPKPKDAPGFPVRAFAIKPSND"
     gene            complement(7758..9986)
                     /gene="metE"
                     /locus_tag="P6L01_000308"
     CDS             complement(7758..9986)
                     /gene="metE"
                     /locus_tag="P6L01_000308"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDP3029409.1"
                     /translation="MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDL
                     HKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGN
                     KDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
                     FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITR
                     EAYDYFEKAGVAKKLVIQTYFERAHLKFLSSLPVGGLGLDFVHDNGYNLKQIEAGDFD
                     KSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLD
                     TSVGEGLSFATEKLDELDALRRLLNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVR
                     TSRQSPFAQRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFL
                     KNEIARWIKIQEDIGLDVLVHGEFERNDMVEFFGEKLQGFLVTKFGWVQSYGSRAVKP
                     PIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKVVQDQI
                     ALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDE
                     TQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDI
                     HSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKR
                     QE"
     gene            complement(9983..11824)
                     /locus_tag="P6L01_000309"
     CDS             complement(9983..11824)
                     /locus_tag="P6L01_000309"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HDP3029410.1"
                     /translation="MSQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVE
                     RIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAANKDTYILG
                     TVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKK
                     YDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIEL
                     PEHAFLSCYPNASLLDIENSEFKYSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIK
                     FIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLD
                     TDRFFENIAKLDKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRN
                     LIGLQSHLLGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGIN
                     TDGDALKKRTHFNIAGAFNPNVRKLDGAVKRLEKKIESGMSYFITQPVYSKEKIIEIY
                     HATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDKAKTRELSL
                     KLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL"
     gene            complement(11793..12953)
                     /locus_tag="P6L01_000310"
     CDS             complement(11793..12953)
                     /locus_tag="P6L01_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HDP3029411.1"
                     /translation="MTLSKETEVIFDWRRGVEYHSANPPLYDSSTFHQTSLGGDVKYD
                     YARSGNPNRELLEEKLARLEQGKFAFAFASGIAAISAVLLTFKSGDHVILPDDVYGGT
                     FRLTEQILNRFNIEFTTVDTTKLEQIEGAIQSNTKLIYIETPSNPCFKITDIKAVSKI
                     AEKHELLVAVDNTFMTPLGQSPLLLGADIVIHSATKFLSGHSDLIAGAVITNNEAISE
                     ALYLIQNGTGNMLSAQDSWTLAKHLKTFPIRFKQSVENAQKIVSFLIKQDEISEVYYP
                     GLTTAHLEQAKNGGAVIGFRLADESKAQQFVDALTLPLVSVSLGGVETILSHPATMSH
                     AALPEEVRQERGITFGLFRLSVGLEDPDELIADIKYALKEAFNESIPHTIER"
     gene            complement(12950..14053)
                     /locus_tag="P6L01_000311"
     CDS             complement(12950..14053)
                     /locus_tag="P6L01_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDP3029412.1"
                     /translation="MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTR
                     TKNPTRSTFETCFAKLEHGIASFATSSGMSAIQLICNLFKPHDEILVSFDLYGGTFRL
                     FEFYEQQYDIKFKYVDFTDYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKA
                     KGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQQLF
                     DFHNMTGATLSPIDSYLLLRGLKTLHLRIERAQSNARKLAKKCQSLQAIDEVLYSGQT
                     GMLSLRLNKAYSVAKLLENLDICIFAESLGGTETLVTFPYTQTHVDMPDAEKDKRGID
                     EYLIRLSLGVENYEDIERDIIQALDKAQIGEIV"
     misc_binding    complement(14117..14486)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            14715..15560
                     /locus_tag="P6L01_000312"
     CDS             14715..15560
                     /locus_tag="P6L01_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDP3029413.1"
                     /translation="MSELSKSEDQRITKTKDEQIKQIDISDIKPNPYQPRKTFDENHL
                     NDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAIIKDLTDEDMM
                     ELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPK
                     KIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSK
                     SETDKVDITKPKFIKQQERQLREQYGTKVDISIKKSVGKISFEFDSQDDFVRIIEQLN
                     RRYGK"
     gene            15714..16595
                     /locus_tag="P6L01_000313"
     CDS             15714..16595
                     /locus_tag="P6L01_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDP3029414.1"
                     /translation="MDQVMSIISSLFEPLTKIETYENIATKIAMIVIYIIVALIVIKI
                     LNKMIEQGFKIQNKSKKSNKKRSKTLISLVQNVVKYIVWFIVITTILSKFGISVEGVI
                     ASAGVVGLAVGFGAQTIVKDVITGFFIIFESQFDVGDYVKINNGGTTVAEGTVKSIGL
                     RSTRINTISGELTILPNSSMGEITNYSITNGTAIVKIPVSVDENIDNVEKKLNKLFTS
                     LRSKYYLFVSDPVVIGIDAFEDTRVILRVSAETIPGEGFSGARIIRKEVQKMFLQEGI
                     KTPQPIMTPFNHSENGV"
     gene            16625..16828
                     /locus_tag="P6L01_000314"
     CDS             16625..16828
                     /locus_tag="P6L01_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDP3029415.1"
                     /translation="MASKYGINDIVEMKKQHACGTNRFKIIRMGADIRIKCENCQRSI
                     MIPRQTFDKKLKKIIVSQDDTQR"
     gene            16840..17937
                     /gene="ychF"
                     /locus_tag="P6L01_000315"
     CDS             16840..17937
                     /gene="ychF"
                     /locus_tag="P6L01_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDP3029416.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIV
                     EVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGASKGEGLGNKFLSHIREVDAICQ
                     VVRAFDDDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEM
                     EVRILTTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDN
                     DKVKAIREYAAQEDSEVIVISAKIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRTTY
                     ELLGLSTYFTAGVQEVRAWTFKQGMTAPQCAGIIHTDFERGFIRAEVTSYDDYVQYGG
                     ESGAKEAGRQRLEGKEYIMQDGDIVHFRFNV"
     gene            complement(18023..18214)
                     /locus_tag="P6L01_000316"
     CDS             complement(18023..18214)
                     /locus_tag="P6L01_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029417.1"
                     /translation="MNLNIDWSKDFQEFQEILNSGIHPEWLYCAKANLVLEPAYTGEG
                     KQFFSTQDIIYASKIIPFF"
     gene            18458..18754
                     /locus_tag="P6L01_000317"
     CDS             18458..18754
                     /locus_tag="P6L01_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDP3029418.1"
                     /translation="MRTYEVMYIVRPNIEEDAKKALVERFNGILATEGAEVLEAKDWG
                     KRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDEDK"
     gene            18775..19278
                     /gene="ssb"
                     /locus_tag="P6L01_000318"
     CDS             18775..19278
                     /gene="ssb"
                     /locus_tag="P6L01_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDP3029419.1"
                     /translation="MLNRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGER
                     EADFINCVVFRRQADNVNNYLSKGSLAGVDGRLQSRNYENQEGRRVFVTEVVCDSVQF
                     LEPKNAQQNGGQRQQNEFQDYGQGFGGQQSGQNNSYNNSSNTKQSDNPFANANGPIDI
                     SDDDLPF"
     gene            19330..19572
                     /gene="rpsR"
                     /locus_tag="P6L01_000319"
     CDS             19330..19572
                     /gene="rpsR"
                     /locus_tag="P6L01_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDP3029420.1"
                     /translation="MAGGPRRGGRRRKKVCYFTANGITHIDYKDTELLKRFISERGKI
                     LPRRVTGTSAKYQRMLTTAIKRSRHMALLPYVKEEQ"
     gene            complement(19849..20787)
                     /locus_tag="P6L01_000320"
     CDS             complement(19849..20787)
                     /locus_tag="P6L01_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDP3029421.1"
                     /translation="MLNFDEQIAKLKQMNIFFNIIDTEKANEILRKNNYFFKLAYFRK
                     NFEKKNGGYFIEFAYLSDLATIDMKLRYTMLHLTLDIEHSLKCLVLKLITENNQEDGY
                     KIIDEFLCIDKSYSNSNFDTNSRTPEEVMETKIKNKNEIFKHMNKRGQLPEKLNKYYQ
                     NPPAWVCIEFMQLGQFVSFLNFYYKKYNDEELRVANILMPLVKNIRNKSAHNQPIIAN
                     LNYDSRSPQYLFEKGNNIGISRNMFGIKNFIDTFATLELHNQVCSNAIIQARYHDLDQ
                     LQKRYKRNESYYNNALAIKRFFIALDKIIDFNRPKV"
     gene            complement(20826..21539)
                     /locus_tag="P6L01_000321"
                     /pseudo
     CDS             complement(20826..21539)
                     /locus_tag="P6L01_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270135.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            21745..22356
                     /locus_tag="P6L01_000322"
     CDS             21745..22356
                     /locus_tag="P6L01_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDP3029422.1"
                     /translation="MFKKYDSKNSIVLKSILSLGIIYGGTFGIYPKADASTQNSSSVQ
                     DKQLQKVEEVPNNSEKALVKKLYDRYSKDTINGKSNKSRNWVYSERPLNENQVRIHLE
                     GTYTVADRVYTPKRNITLNKEVVTLKELDHIIRFAHISYGLYMGEHLPKGNIVINTKD
                     GGKYTLESHKELQKDRENVKINTADIKNVTFKLVKSVNDIEQV"
     gene            22725..23093
                     /locus_tag="P6L01_000323"
     CDS             22725..23093
                     /locus_tag="P6L01_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDP3029423.1"
                     /translation="MKTILKTITYLALTIIGAYAALFILKTIDSHGITDQFNPLVKED
                     DSYVKTTEVSTRMDDQLRSYTQSAFNKEGKETQLMYTATFDVKPHRYLKITHKGHHVE
                     TFEEVEKEEVPKKALDKLSR"
     gene            23274..23846
                     /locus_tag="P6L01_000324"
     CDS             23274..23846
                     /locus_tag="P6L01_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDP3029424.1"
                     /translation="MKLKSLAVLSMSAVVLTACGNDTPKDETKSTESNTNQDTNTTKD
                     VIALKDVKTSPEDAVKKAEETYKGQKLKGISFENSNGEWAYKVTQQKSGEESEVLVAD
                     KNKKVINKKTEKEDTVNENDNFKYSDAIDYKKAIKEGQKEFDGDIKEWSLEKDDGKLV
                     YNIDLKKGNKKQEVTVDAKNGKVLKSEQDH"
     gene            complement(23983..24246)
                     /locus_tag="P6L01_000325"
     CDS             complement(23983..24246)
                     /locus_tag="P6L01_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDP3029425.1"
                     /translation="MNMHILYNLRTKHNLEIDELAQQLNEKYGTKYEAHQIWEWENHH
                     HEPKFKDAMHLADFFDAPYEMFLESKVKEYQKHLEEVDIRMDK"
     gene            24546..24797
                     /locus_tag="P6L01_000326"
     CDS             24546..24797
                     /locus_tag="P6L01_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDP3029426.1"
                     /translation="MFGFIGMLIVGGLIGWAAGAIMGKDIPGGILGNIIAGIIGSWVG
                     GKLFGQWGPELGSIYILPALIGSIILIAIVTLILRAMRK"
     gene            complement(24836..25045)
                     /locus_tag="P6L01_000327"
     CDS             complement(24836..25045)
                     /locus_tag="P6L01_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029427.1"
                     /translation="MTTQSFIFMNIILLDGATQRHPFFICQILAFLFPKFPHINLLLF
                     YPILSTFSNIIIHIDHFHANKKDDN"
     gene            25223..25804
                     /locus_tag="P6L01_000328"
     CDS             25223..25804
                     /locus_tag="P6L01_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDP3029428.1"
                     /translation="MTIYLVRHGESKSNYDNKHFRSYFCGQLDVPLTDTGTKSADDLC
                     DYFKEKQIKHVYVSDLLRTQQTFEHIFPYDIASTTTPLLRERSLGVFEGEYKDEISAN
                     PKYEKYFNDPNFKDFRHSFSQKAPEGESYEDVYQRVEHFMNHVVNEDTQKDDIVIVAH
                     QVVIRCLMVYFNKVSREEAVDLKVENCKPYIIE"
     gene            complement(25869..26252)
                     /locus_tag="P6L01_000329"
     CDS             complement(25869..26252)
                     /locus_tag="P6L01_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029429.1"
                     /translation="MKVRITFIILAILSTIVCLFLAAMHPTGPNTVTFEQPYLFTLNI
                     ILMVLVALPSLVLAIYDYMSFRISSAILQFLGAISWFFLSLILSLTQFTPFTLASFIT
                     SIILFISAIITLAIGGKSVDKNDSH"
     gene            complement(26522..27148)
                     /locus_tag="P6L01_000330"
     CDS             complement(26522..27148)
                     /locus_tag="P6L01_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDxxF motif lipoprotein"
                     /protein_id="HDP3029430.1"
                     /translation="MKKRLLLSTFLASALILTGCASDQSDNEDHHTSTGIHAPKSAKK
                     LETKDIFNSDKKNSDISDAEMKQAIEKYLSVNSDILDNKYIMQHKLDKQIDSQTKVTE
                     KQAETLSHLSNLAVKNDLHFKKFVTENNIPKEYKKPVELMMNYFKALNSTIANVDEDI
                     EKLSYQPQNKINVVDVPTKYAGDVNKKQQDKIKDFLKSKGIKSDVIDK"
     gene            complement(27221..27439)
                     /locus_tag="P6L01_000331"
                     /pseudo
     CDS             complement(27221..27439)
                     /locus_tag="P6L01_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27592..29115)
                     /gene="ahpF"
                     /locus_tag="P6L01_000332"
     CDS             complement(27592..29115)
                     /gene="ahpF"
                     /locus_tag="P6L01_000332"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDP3029431.1"
                     /translation="MLNADLKQQLKQLLELMEGNVEFVASLGSDEKSKELKELLTEIS
                     DMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKE
                     KQSIIDQIKNLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
                     SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASG
                     SAAIYTARKGLRTGIVADRIGGQVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDID
                     AMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLNIPGEEQLINKGVAF
                     CPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRS
                     LSNVDIKTNAKTTEVVGEDHVTGIRYEDMSTGEEHLLNLDGIFVQIGLLPNTSWLKDA
                     VELNERGEIVIDRNNNTNVPGIFAAGDVTDQKNKQIIISMGAGANAALNAFDYIIRN"
     gene            complement(29131..29700)
                     /gene="ahpC"
                     /locus_tag="P6L01_000333"
     CDS             complement(29131..29700)
                     /gene="ahpC"
                     /locus_tag="P6L01_000333"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDP3029432.1"
                     /translation="MSLINKEILPFTAQAFDPKKDQFKEVTQEDLKGSWSVVCFYPAD
                     FSFVCPTELEDLQNQYEELQKLGVNVFSVSTDTHFVHKAWHDHSDAISKITYTMIGDP
                     SQTITRNFDVLDEATGLAQRGTFIIDPDGVVQASEINADGIGRDASTLAHKIKAAQYV
                     RKNPGEVCPAKWEEGAKTLQPGLDLVGKI"
     gene            30192..30947
                     /locus_tag="P6L01_000334"
     CDS             30192..30947
                     /locus_tag="P6L01_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HDP3029433.1"
                     /translation="MSDYVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFL
                     QAYSIIGIDDEKIKENLREVSGQPYVVENGYLFVFVIDYYRHHLVDQHAETDMENAYG
                     STEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFG
                     MAVGEPADDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGN
                     RKETWSQQIEMFLGNKARLDMLEQLQKSGLIQR"
BASE COUNT        10387 a         4650 c         5670 g        10254 t
ORIGIN      
        1 taatccaggt gcaatgaatg cccacaattg ataaaacatg actggtgaaa tgaaacaaaa
       61 cgcgatgaaa aatataatca tcacgtatat ttggatcatt tctgtgaatg aaaatgcatg
      121 taaggacaca tgtgcacggg taatatacgt tatgaatggt gtcatccacc aaaatgatga
      181 aacatatacg acgatgactg tgacgacgaa cgacaatagt attttaacta aacgatgacg
      241 tagctcgcta aaatgaacta gtaaggtgtg gtcagtgcta ttgctctcgc tgttgtttcg
      301 attccttact gggtgtgtcg tgagactctt tgtctaaatc ttctgttgca gatttaaatt
      361 cttttaaagt agaaccgata gcacgaccaa attgtggtaa ttttttcgga ccaaaaataa
      421 ttaaagcgat aatgctaatg acgacaagac ttgttgggcc tgtgatgcct aaaataaaag
      481 tgttagttat catgataatc aacctcactc ataagtagta tatagtattt actttatacc
      541 gaacgataat gattatcaat aaaattttat aaaataatta aaatgttgct gtaaatacaa
      601 attactgagg tattaattct gaaataatag cttgttcttt caagtcgata taagtacaag
      661 taccagcttg aatatcgata tgccatttat aaatgccagc ttcagccatt tcatcacaaa
      721 atgtttcgaa atctgtttgt ccttgttgat gtctagttaa tacatcttga actattgttt
      781 tgtttgattt ttgagcaaca ggatgattac ttttcacaga tgacgtaacg atatcatctt
      841 ctgattgatg tacgtatgtt gcagtgccat cttgaatgtt gacgatattg taagtcatcc
      901 ccatatcttt aaaagctttg aatagttttg gaaagtcaac accagtaaat tgttgatgtg
      961 cttgttgaat tgcagataat gtaaatgcca taatagtctc ctttgaatgg tttgatttag
     1021 tgaaaatgcg tgcatcatac taaaattaga atgctgttaa ttatgcagga aggtgtaacg
     1081 aaagtaaaca attatgactt gtaggcgttg aggtgttgtt tcaaaatgtc atacataggt
     1141 ggacaatcat atttttttgt gtagatttga caaatatagt tgtcaaaaag acaaatatca
     1201 tttacattag gactaaggag ttgatgcgat gtgcgtaatc gattgaaaga gttacgagca
     1261 cgagatggct taaatcaaac gcagcttgcc aaactagctg gtgtttcaag acaaaccatt
     1321 tcgctaattg agcgaaacaa ttttatgcca tcagtattaa cggcaataaa gattgctcgc
     1381 attttcaatg aaacggtgga aacggttttt attattgagg aggatgaagc atgaaaatac
     1441 taagatatat cggatatctt ttactgggtg gaattgtagg gggtatcatt ggtggaattt
     1501 taggtaactt tgatggattg ggtattgaga acttgacgtt tgcgacttat aacaatgtcg
     1561 ttgtaatatc gattgttgcg actatgatca tcatattggt agaagccatt gttttgatga
     1621 atcaaagacg tgcattgaag tataagcgac ttgtagatga agaggtagat atcgatgcaa
     1681 cagatcaata tgaattgctt gcgaatcgtt atgttttaaa tggaagtata ttaagcgtta
     1741 tacagacaat tattgccttt gtagtgttac taatttttgt ggtagggcaa gctgaagcaa
     1801 atgcaatgct attcttttta ataccatttt ttgctagtgc tattttcaat acacaattta
     1861 cactgtttaa tagaaaattt gatgacagaa tgccaaaaat tgcagataag aattacactg
     1921 aaaagcgatt ggaaatattg gatgaaggtg aacgccatat agaattaatt gcattattta
     1981 aaacatatgc gatcaactta tcaatattga tactagccat tatttttata gggtcttatt
     2041 caattgctac aggaattaat caaagcttta gtttgctact tatcattgct attttaatat
     2101 ataacgcctt tagctattta ttgaagagaa gacgttttta ttaaaattaa acaaagagga
     2161 gcatggatat gacaacattg ttaaacgtag atcgtgtgaa caaacaatac aaagattcgg
     2221 attttaaatt gcaagatgca tctttaacga tttctactaa tgagacagtt ggattaattg
     2281 ggaaaaatgg ctcaggtaaa tcgacattaa ttaatattct agtaggcaat cgacataaag
     2341 ataacggtag tattgcattt tttggagaag aacatgctgc ggatgatgtc gaatataaag
     2401 aacacatagg tgttgtgttt gatgatctga gagtacctaa taaattgact attaaagata
     2461 ttgataaagt atttcaatct atttatacga cttggaatag tcaaaaattc tttgatttaa
     2521 tcaaatattt cgagttacca ctacaaacta aaattaaaac tttttcaaga gggatgcgaa
     2581 tgaagatagc tttaacgatt gcgctttctc atgatgtgaa gttattaatc ttagatgaag
     2641 caactgcagg tatggatgtt tctggaagag aagaagtaat ggaactatta gaagattttg
     2701 tcgctcaagg tggaggcatc ttaatatcat cgcatatttc tgaagatata gaacaattag
     2761 cggataaatt agtgtttatg aaagatggac gaatgatttt aactgaacag aaagatatac
     2821 ttttagcaca atatggaatt gttacgacag gagataaaga tgttgaaatt cctaagcatt
     2881 taatcattgc ttctagattg tcaaagggga aatatcaaat tttagttaaa gattatgcag
     2941 aaattgaaaa tgcagagcct ttaaaacaca ttgatgacgc tacgaaaatc ataatgcgag
     3001 gtgaagtata atgaaaggta tgttcctaag tagtttttat gcaacgagaa agcaaacata
     3061 tatttatttt atagtcgcta tcatagctgc gggatacttt gcagtattta atccgttgat
     3121 gagttcggca atggctgggg ttatgttaat cacacccatt actgataata ttaaacatga
     3181 aaaagactca agatggatgt attatgtatc tactttaccg gttaaacgta gtgattatat
     3241 taagtcatac tttgcctttt atttaatctt attcggtgca agtttaatga ttggattagt
     3301 tgtgactacg atcgtgaccc aaagtgtgat gattggtatt atgtcaggtt taatgagttt
     3361 tggtatcata ggggcatact ctatcatttt cccattgaca tttaaatttg gcgctgaaaa
     3421 ctctaatgtc attatgatag ttgcatctat cctactactt ttttctttcg tggtcttttt
     3481 ctttatatat ggtatggtta gtggtgcatc ggcattagaa tttgaaaaaa ttagcactga
     3541 aggatggtta gttgtcatag tatatgcggt cattggtatc gttataacaa gtgtttctta
     3601 tatattgtct attaaaattt ttaacaaaca agaactataa tcaatattct tggtgtgaag
     3661 ctagtcattg aaataatatt tagagcaggt gataaatcaa tacgattatc atctgctctt
     3721 tttggtgttg tggaatgaaa tgcgggatga taggttgagg tgacatctct atatcatatc
     3781 atttgttttt agaagattag gttatgtggg aattgcatcc ttttatagtg aggattaacg
     3841 tgtatatatc attgagtatg ttgctgaaat ttatttgcgt gtatcatttg atgattgtat
     3901 aggctaacga tttccttgga aatatttaaa aacctcgatc atgtagcata actgaatcca
     3961 aaagtataat gtgaagttcg acacttttgg attcagttca aatactttga ccgaggtgaa
     4021 tactatttat tcatgtttat tacgtagacg ttgattttta aaataaatca gcatgatgca
     4081 gaaagtcgca acggataccg agataatcat tacttggtca tgccccttaa agataaggct
     4141 gatgatatat gatactacaa gtatgcctag tgaagaagaa atatattttg cgtttgtcag
     4201 ttcattatgg aaataaggcg tgattaacca taatccaata tagaatatta aaacactgat
     4261 atacatcata ttaatttcaa acaagtcatt tagtttattg ttattactaa aaacaattgc
     4321 agcattaatc acacctaaag cgatattgat taatagatgc gtatacgata aacggaaacc
     4381 gatagatgac aatttatgat taatgtaatt ttcagtaatg atccaatata caccgaaaag
     4441 actaattaaa atcataaatt ggaatatata aatgtaacta aaatgatcaa tgctaaatga
     4501 tgacgaagct aaaccaacca gtacctcgcc aaagataata attgttagta acgaaaaacg
     4561 ttctactaaa tgcatcatat taacaggtga taatacaaga tatttctgaa atggaataag
     4621 tcctgtcgct gcaatgaata cgcctaaaaa tccagggatg taatggatac tttgtggtag
     4681 tactaatgat agaaatgata aaaatgaaat cacaaaggct acgctcgcaa aagcttgaca
     4741 tgtacgctta tcgccataat ctaaccctgt acgtatatgt aataaatact gtaatccgat
     4801 acttaaatac ataattgcca cgcataagaa gaatgggaag aatgtctttt caaagtccgg
     4861 atataggctg ttagatagga agaccatgat gaacatatta aacatcataa acgagacgtc
     4921 tttgaatgta acttgaccaa atcgatttgt aaaaaatgtt tgatgagacc acattaacca
     4981 taagaacaaa ctcatgacga tgtatttgaa aaataaatca gctgaaatgg aaccgttttg
     5041 tgttgttaaa atcacatgtg caattttttg aatggcatag acgaaaatta aatcaaagaa
     5101 caactcatgg aatcctgcac gcttttcagc taaatgtttt ggtgttaatg cattaaccat
     5161 aaaattttaa ctcctttaag atgtgtaatt aatttactaa gtatactatt tattttttct
     5221 agtgaatagg ggcagatttg gcgatgaagt ggaaggagag gtgactgcaa ggtaattgcg
     5281 gaattaacaa tcatcagcga tttaatattt gactggagac gtcatggtaa taaaaaattg
     5341 atgagaaatt gatggtgaaa ccagctgtga atagcgatgc aatgatagat agaatttaat
     5401 tagagtcatt acgcgaaatg attaatgata atttgtggta aatcaaagca taattttgta
     5461 ctatagatga ggatgataga gcatatttaa gagtgtgaaa tgttaaagtg aaaccgttta
     5521 cgtttccgat tgcccaaaca aattacatca ttgtataata tgatttgtta aatgcataac
     5581 aagaatgaaa atgtaacata cgtagcaatt ggtttcataa attggatgtt agtggcgtat
     5641 tggttcatta gacgtattag taataaaatt gtatatatca taaggagatg aatatgacat
     5701 gacgagagtc gtattagcag cagcatacag gacacctatt ggcgtttttg gaggtgcgtt
     5761 taaagacgtg ccagcctatg atttaggtgc gactttaata gaacatatta ttaaagagac
     5821 gggtttgaat ccaagtgaga ttgatgaagt tatcatcggt aacgtactac aagcaggaca
     5881 aggacaaaat ccagcacgaa ttgctgctat gaaaggtggc ttgccagaaa cagtacctgc
     5941 atttacagtg aataaagtat gtggttctgg gttaaagtcg attcaattag catatcaatc
     6001 tattgtgact ggtgaaaatg acatcgtgct agctggcggt atggagaata tgtctcagtc
     6061 accaatgctt gtcaacaaca gtcgcttcgg ttttaaaatg ggacatcaat caatggttga
     6121 tagcatggta tatgatggtt taacagatgt atttaatcaa tatcatatgg gtattactgc
     6181 tgaaaattta gtagagcaat atggtatttc aagagaagaa caagatacat ttgctgtaaa
     6241 ctcacaacat aaagcagtac gtgcacagca aaatggtgaa tttgatagtg aaatagttcc
     6301 agtatcgatt cctcaacgta aaggtgaacc aatcttagtc actaaggatg aaggtgtacg
     6361 tgaaaatgta tcagtcgaaa aattaagtcg cttaagacca gctttcaaaa aagacgggac
     6421 agttacagca ggtaatgcat caggaatcaa tgatggtgct gcgatgatgt tagtcatgtc
     6481 agaagacaaa gctaaagaat taaatatcga accattggca gtgcttgatg gctttggaag
     6541 tcatggcgta gatccttcta ttatgggtat tgcaccagtt ggcgctgtag aaaaggcttt
     6601 gaaacgtagt aaaaaagaat taagcgatat tgatgtattt gaattaaatg aagcatttgc
     6661 agcacaatta ttagctgttg atcgtgaatt aaaattacct cctgaaaagg tgaatgttaa
     6721 aggtggcgct attgcattag gacaccctat tggtgcatct ggtgctagag ttttagtgac
     6781 attattgcat caactgaatg atgaagttga aactggttta acatcattgt gtattggtgg
     6841 cggtcaagct atcgctgcag ttgtatcaaa gtataaataa taagaaaaca ggttatcaca
     6901 acagtattaa ttacatgttg gcataacctg tttttatttg tttatggatt tattgggtaa
     6961 tattagtcat ttgatggttt aattgcaaat gctctaacag ggaacccagg tgcatctttt
     7021 ggtttagggc tgatagcgta aatgatggcg ccacgagttg gtaattgatc taaattagtt
     7081 aataactcga cttggtattt atcctgacca agaatataac gttcgccaac taaatcacca
     7141 ttttttacaa cgtccacaga tgcatcggta tcgaatgttt catgaccaac agcttcaaca
     7201 cggcgttctt caattaagta cttcaaagca tctaatcccc aacccggtgc atgttgttgt
     7261 ccgttcgcat ctttgttttc aaacttttca atattaggcc aacgttttga ccaatcggta
     7321 cgaagtgcaa caaaagtgcc aggttcaata gtaccatgct ctttttccca tgcttctata
     7381 tgcgcacgtg ttacgatgaa atcattgttg ttcgctactt ctgttgaaaa gtctaataca
     7441 attaacggca ataccaattc ttttaaatca atgtcttcta aataacgttt gttctcgaca
     7501 aagtggattg gtgcatcaat gtgagtacca tattgcgtta caatattcca acgttgcaca
     7561 tagaaaccat gatctttaac cgtgaataaa gttgaaactt cacctttttc aaactcacta
     7621 aaacgtggta tttctggatc aaatgtatgc gttaaatcaa cccaagttgc ttgttttaaa
     7681 gtatttaatt gttgccataa aggatattgt gtcataaaat cacccgtttt tagtttatta
     7741 tatgataaat gctgcgatta ttcttggcgt ttagctttaa cagcattcac aagtacagtc
     7801 aatgcatctt taacttcttc ttcttttcgc gttttcaaac cacagtcagg gtttacccag
     7861 aataatgagc ggtcgatttg ttgtagtgaa cgattgattg ctgtagtaat ttcttctttt
     7921 gttggaatac gtggactatg aatatcatat acacctagac cgatacctaa atcataatta
     7981 atatcttcaa agtctttaat taaatcacca tggctacgag atgtttcaat tgaaataaca
     8041 tcagcatcta agtcatgaat agcatgaatg atttgaccga attgagaata acacatatgt
     8101 gtatggattt gagtttcatc acgaactgaa gacgttgcaa gtttaaatga taaaacagca
     8161 tctttaagat attgttcgtg atattcagag cgtaatggta agccttcacg taatgcaggt
     8221 tcgtcaactt ggataacttt gattcctgca gcttcaagtg ctaatacttc ttcgttgatt
     8281 gctaaagcaa tttgatcttg aacgacttta cgtggtaaat caacacgttc aaatgaccag
     8341 tttagaattg ttacaggtcc agttaacata cctttaactg gtttatctgt taagctttgt
     8401 gcataaactg tttcatcaac agttaaaggc gctgtccatt ttacatcacc ataaatgatt
     8461 ggtggtttta cggcgcgtga accatatgat tgcacccaac cgaatttagt tactaagaaa
     8521 ccttgtaatt tttctccgaa gaattcaacc atgtcattac gttcaaattc accgtgaact
     8581 aatacatcta agccgatgtc ttcttgaatt ttaatccatc gagcaatttc attttttaag
     8641 aatgtttcat atgcttcgtc tgtaatgcgt ttgttcttcc aatctgcacg gtattttcga
     8701 acttctcggc tttgtgggaa tgatccaata gttgttgttg gtaaatccgg taagttcaaa
     8761 cgtttttgtt gttgttcaat acgttgcgcg aatggtgatt gtcttgaagt acgcacgctt
     8821 tcgaaatcat aatctaagtt tttgaatgat tgattttgga aacgctcata acgtgctttt
     8881 aatttatcat atttaacact atcgttttga ttcaataggc gacgcaatgc atctaattcg
     8941 tctaattttt cagttgcaaa gcttaagcct tcgccaacac ttgtatctaa tgtttcatca
     9001 tctaaagata ctggaacatg taataatgaa gatgatggtt gaatgacaag ctcattagtg
     9061 tgtgctaaca atttgtcgat taagactttt ttagcttcaa tatcacttgc ccatacatta
     9121 cgaccatcaa taattccagc gtataatgtt tttgatttat caaaatctcc tgcttcaatt
     9181 tgtttaaggt tatagccatt atcatggaca aagtctaaac ctaaaccacc aacaggtaaa
     9241 gaacttaaga atttaagatg tgcacgttca aagtatgttt gaatgactaa ttttttagca
     9301 acaccagctt tttcgaaata gtcataagct tcacgtgtaa tattttcata gctttcgctg
     9361 tcgtctgtaa ctaagattgg ctcatcaact tgaatgtact cagcacctgc atcaattaat
     9421 gattcaaaca cttctttata aagtggtaat aacgttttaa ctttttcttc aaaagtttgg
     9481 tgaccgcctt ttgataattt aacaaaagta atcggaccaa caatgacagg gtgagcgtta
     9541 acgtttaaag attgggcata tttaaagcga tctaataata cattgcgact cactttaggc
     9601 tcaacattgt cccattcagg tacgatgtaa tgatagttag tgttaaacca ttttataagt
     9661 gcacttgcaa catggtcttt attaccgcga gcaatatcaa ataataaatc atcatcaata
     9721 gttcttcctt ggaaacgttc agggatgatg ttgaataata atgacgtatc taatatatgg
     9781 tcatataaag agaaatcacc aactgggatg ctatctaagt gatagtactt ttgtaataat
     9841 aaattttctt tatgtagatc agttaatgtt tgatctaatt cttctttaga aatcttcttt
     9901 gcccaataac tttcgatggc ttttttccat tctctttttc tacctaatct tgggaatcct
     9961 aagtttgatg ttttaattgt tgtcataata ttgcctcctt gtgagcagta atagattttg
    10021 agtatgctgc aagttctaat gaatcttcga cattttgaaa cggtgtgata atgtataaac
    10081 cattaaaata ttcatgaaca gtatcgatta aatcctttga aagcttaaga cttagttctc
    10141 gtgttttggc tttatcatct ttaactgctt caaattgttg taaaatttca tctgacatct
    10201 tgattcctgg cacttcatta tgcaaaaaga gtgcgttttt gtaacttgcg ataggcataa
    10261 tgcctatgaa aaatggtttg ttcaagtgct tagtggcatg gtaaatttca atgattttct
    10321 ctttgctgta cacgggttgt gttataaaat aagacattcc gctttctatc tttttctcta
    10381 atcttttgac ggcaccatct aatttacgaa cattagggtt aaaggcgcca gcgatgttga
    10441 agtgtgtacg tttcttcagc gcatcaccgt cagtgttaat accttgatta aatcttagag
    10501 cgagttcagt taatccttta gaattaacat catagacatt ggttgcacct ggtaagtgac
    10561 caacttttga aggatcacca gttatggcta atatttcgtt aacgccaatg agcgataatc
    10621 caagtaaatg ggactgcaag ccgattaagt ttcggtctcg acatgtaata tgtacgagtg
    10681 gttcaatatt gtaatattgc ttaattaagc tagcagcagc aatattgcta attctgacag
    10741 ttgccaatga attatctgcg agtgttaccg catctacatt agctttatca agtttagcga
    10801 tattttcaaa aaatctatcc gtgtctaaat gtttcggtgt atccaattcg ataataacgg
    10861 ttggacgttc ttgaacctta gatgttaatg attgtctaac tttattttga gatggattga
    10921 aaagtgcttt cgttggtatc ggaatcactt ttttgtcatt aacaggttta agtgtctgaa
    10981 tagattcttt aataaatttg atgtgctctg gcgttgtacc acagcaacca ccaattaaac
    11041 gaacaccttc gcgaattaga ttttgagcaa cttgaccgaa atattgtgca ttgtcactat
    11101 acttaaattc actattttca atatctaata agctggcatt tggataacaa gataagaatg
    11161 cgtgctctgg taattcaata tgtgtgaaag actcttgcat atggtgcgga ccatgatgac
    11221 aattgagtcc cacgatgttt gcaccacatt gaacgagttg ttttaatcct tcattgattg
    11281 cctgaccatt aactaagtaa tttgtgtttg aagcggttaa ttgagcaatg attggaatgt
    11341 cgtatttctt tctcgttcgt gaaatgacat ttgttaactc ttctaggtcg taatacgttt
    11401 cgaaaagtag cgcgtcaacg ccttcttcaa ttaaggtgtc tatttgaatt tcagtatgat
    11461 aaagaatagt ttgtaagctg atatcctctt gtttgatacc tctaaaccca ccaactgtgc
    11521 ctaatatata cgtatcttta tttgctgctt tttttgcgat gcgaacggcg gcttgatgta
    11581 ttgctttaac tttatcttca agaccgaatc gttttaactt ttcaaaattt gcaccataag
    11641 tattggtttg aatgacatca gcaccggctt caatatatga acgatggatg cgttcaactt
    11701 tatctggatg gctaagatta tatgcttctg gacaggtgtc taatccttca gagtataaaa
    11761 tggttccaat agcgccatca gctactaaaa cattatcttt caattgtgtg aggaattgac
    11821 tcattgaatg cctcctttaa tgcgtatttg atgtctgcaa tgagttcatc aggatcttcg
    11881 agaccaacac ttaatcggaa tagaccgaaa gtgataccac gttcttgtct cacttcttca
    11941 ggtagtgcag cgtgagacat tgttgctgga tgtgaaagga tcgtttcaac accacccaga
    12001 ctcactgaaa cgagtggtaa tgtcagtgca tcgacaaatt gttgtgcttt agactcatca
    12061 gctaaacgaa agccaataac ggcaccgcca tttttagctt gttctaaatg agcagtagtg
    12121 agtcccggat aataaacttc tgaaatttca tcttgcttta ttaaaaatga cacgattttt
    12181 tgcgcgtttt cgacagattg tttaaatctg attggaaatg tttttaaatg tttagcaagt
    12241 gtccagctat cttgagcaga taacatattg cctgtaccat tttgtattaa ataaagagct
    12301 tcactaattg cctcattatt agtaatgaca gcaccagcaa ttaaatcgct atgtccactt
    12361 aaaaattttg tagcactatg aatgacaata tcagcgccaa gtaataaagg tgattggcct
    12421 aacggtgtca taaatgtatt gtccacagct accagtagtt catgcttttc ggctatttta
    12481 gaaacagctt tgatatcagt aattttaaaa cagggattcg atggtgtttc gatataaatt
    12541 aattttgtgt ttgattgaat ggcaccctcg atttgttcga gcttggtagt atctacggtt
    12601 gtaaattcaa tattaaatcg attcaaaatt tgctcagtga ggcgaaaagt accgccatat
    12661 acatcatcgg gtaagatgac atgatcacca gatttgaaag tcaaaagtac tgctgaaata
    12721 gcagcaatac ctgatgcaaa agcaaaagcg aattttccct gttctaatcg tgctaacttc
    12781 tcttctaaaa gttcacggtt agggttgcca cttcgtgcat aatcatattt aacatcgcca
    12841 ccaagacttg tttgatggaa tgttgaagaa tcatagagtg gtgggttagc tgaatgatat
    12901 tccacacctc tacgccaatc gaatatcact tctgtctctt ttgaaagtgt catacaatct
    12961 ctccaatctg agctttatct aatgcttgga tgatatcgcg ttcgatgtct tcataatttt
    13021 caacacctag tgataagcgg attaaatact catcaatgcc acgtttatct ttttcagcat
    13081 ctggcatatc aacatgtgtt tgggtgtaag ggaaggtcac taatgtttca gtacctccta
    13141 aactttctgc aaaaatacaa atgtctaaat tttctaataa tttagcgacg ctataggcct
    13201 tgttaagtct taaactaagc atgccagttt gcccgctata tagtacttcg tcaattgctt
    13261 gaagtgactg acatttttta gcaagttttc tagcgtttga ttgcgcacgc tcaatgcgta
    13321 aatgcaaagt tttaagtcca cgtaacaaca aataactatc tattggtgaa agtgttgcgc
    13381 cagtcatgtt gtgaaaatca aacaactgtt gcgcgagtga ttcatctttg acggttacga
    13441 cacctgctag tacatcgtta tgtccaccaa tatatttcgt agctgaatgt aagactatat
    13501 cagcaccttc tgctaatggt gttgaaagat aaggtgttaa aaaagtattg tcgataattg
    13561 acaataagcc tttagcttta caaagttgat agtacggctt tacatcaata gcaatcattt
    13621 gtgggttaga tattggttca atgaataatg caactgtttt atcagtgatt tctttttcaa
    13681 cttgttcata atctgtaaaa tcaacgtact taaatttgat atcgtattgt tgctcgtaaa
    13741 attcaaataa tctaaatgtg ccaccatata aatcgaatga aactaaaatt tcatcatgag
    13801 gtttaaatag attacatatt aattgaatgg ctgacattcc acttgatgta gcgaatgatg
    13861 caataccatg ctcaagtttg gcaaaacagg tttcaaatgt tgagcgtgta ggatttttag
    13921 tacgtgtata atcaaatcct gtcgattgtc ctagttttgg atgtttgtag gcagtagata
    13981 aatggattgg attcgctata gcaccggttg aatcatcagt taatgtgatt tgggctaact
    14041 gtgtatcctt catattaaga ccctcctata agaaaaaata aaaagcttcc gtccttcgta
    14101 cccgaatgaa tcggataaaa aggacgaaag cttatgtttc gcggtaccac ctttatttgt
    14161 tattccatcg ctgaaataac cttattcagt acgcattaaa agtaaatatg cttactgaac
    14221 aattatcaca attaaagtca gtaagtaagg atatagtaat gtgctatccc atacttatta
    14281 acaaaaaatc gtgcgtaaag aatccagtac gccatttaac atcaatgtta atactgtatc
    14341 gctataacgg gcgaacccgt agacacctca tattggcatc aacactccaa ggccattttc
    14401 aaacacgctt tcaaaatctt ctctcagcta ctaaagactc tctgtataag cagggtgtgt
    14461 tttactttcc tctttattgt gtttacgttt cattaaacta ttataagata ttaattagct
    14521 tacagagtaa aaaaagattt gtcaacaatt attcagaaaa ttttgattta aaagttaatt
    14581 tgtttgtgaa attgtaattg gtatcttgaa gttgaaaaat gaattatttt ttaaataaag
    14641 tgtggttaat ggttgtctga ctcatttaga atacataaaa tatatttaac tgttgttatc
    14701 aaataaaaag tgatgtgagt gaattgtcaa aaagtgaaga tcaacgtatt actaaaacaa
    14761 aagatgaaca aattaagcaa atagatatat cggatatcaa accgaatccg tatcagcccc
    14821 gaaaaacttt cgatgaaaat catttaaatg atttggcaga ttcaattaag caatatggaa
    14881 ttttgcaacc aattgtgctt agaaaaacag ttcaaggtta ttacattgta gttggtgaaa
    14941 gaaggtttag agcttcgaaa attgctggtc taaaatacgt atcagcgatt atcaaagatt
    15001 taacagatga agatatgatg gaactggcgg tcatcgaaaa tttacaacga gaagacttaa
    15061 atgcgattga agaagctgaa agttatcaac gtttgatgac agatttgaaa attacacaac
    15121 aagaagtagc gaagcgattg agtaagtcgc gcccgtatat agcgaatatg ttgaggttat
    15181 tacatttgcc gaaaaagatt gctgacatgg taaaagatgg gcgactgaca agtgcacatg
    15241 gacgaacgtt attggcaatt aaagatgaac aacaaatgct taggttagcg aaacgggttg
    15301 ttaaagaaaa gtggagtgtt agatatttag aaaaccatgt taatgaatta aaaaatgttt
    15361 cgtcaaagtc ggaaacagac aaagtagata taactaagcc taaatttata aagcaacaag
    15421 aacgacagtt gcgagaacag tatggtacca aagtagatat atcaataaaa aaatcggttg
    15481 gtaaaatctc atttgagttt gattcacaag atgactttgt gagaataatt gaacaattaa
    15541 atcgtaggta tggtaaatag ttacacaatt ttatataata actctttgtg aatctataga
    15601 tatattgata gttatctata ttctttgaag atgatgcgac ttcagtaggt tggaagaata
    15661 caacaatatt aataaaatta tttgagacta tatagaaggg agtaacgcta attatggatc
    15721 aagttatgag tattatttca tcgctattcg aaccattaac taaaattgaa acatatgaaa
    15781 atattgcaac taaaatcgct atgattgtta tttatattat cgtagccctc atagttatta
    15841 aaatactgaa taaaatgatt gaacagggat ttaagattca aaataaaagt aaaaagagta
    15901 acaaaaagcg ctctaaaact ttaatatctc ttgttcaaaa tgtagtgaag tatatcgttt
    15961 ggtttatagt tattacgacg attttaagta aatttggcat tagtgttgaa ggtgttattg
    16021 caagtgctgg tgtcgtaggt ttagcagtag gttttggtgc gcagaccatt gttaaagacg
    16081 ttattactgg atttttcatt atatttgaaa gccaatttga tgtaggtgat tatgttaaga
    16141 taaacaacgg tggtacaact gtagcagagg gaacagttaa atcaattgga ctccgttcaa
    16201 cacgtattaa tacaatatct ggagaactta caatcttgcc aaatagtagc atgggtgaaa
    16261 taacgaatta ctcaattacg aatggaacag caattgttaa aattccagta tctgtagatg
    16321 aaaatattga taatgttgag aagaaattaa acaaattatt tacatctctg cgtagtaaat
    16381 attatttatt tgtaagtgat cctgttgtga ttggtattga tgcctttgaa gatactagag
    16441 taatattaag ggtttcagca gaaacaatac caggtgaagg tttctctggt gcgcgtatta
    16501 ttcgtaaaga agtacaaaaa atgtttttac aagaaggtat caagacacca caaccaataa
    16561 tgacaccttt taatcatagt gaaaacggtg tttagtagtt tataatacat ggaggtcata
    16621 tttaatggcg tcaaaatatg gaataaatga tatagtagaa atgaaaaaac aacatgcgtg
    16681 tggaaccaat cgttttaaaa ttattagaat gggtgctgac attagaatta agtgtgaaaa
    16741 ttgtcaacga agcattatga ttccacgtca aacgtttgat aaaaaactaa aaaaaatcat
    16801 cgtatctcag gatgatacac aaagatagga gaatgattca tggctttaac agcaggtatc
    16861 gttggcttac caaacgttgg taaatcaaca ttatttaatg caattacaaa ggcgggtgcc
    16921 ttggcagcaa actatccgtt cgcaactata gacccaaatg tgggtattgt agaagtgcca
    16981 gatgcaagac tacttaaatt agaagaaatg gttcaaccta aaaaaacatt acctactaca
    17041 tttgaattta cagatattgc cggaattgtt aaaggtgctt ctaaaggtga aggtttaggt
    17101 aataaattct tatcacatat tagagaagta gatgcaattt gtcaggttgt tcgtgctttt
    17161 gacgatgata atgtaacaca tgtagctggt cgagttgatc caattgatga catcgaagtt
    17221 atcaatatgg aattagtact tgcagactta gaatcagttg aaaaacgctt acctagaatt
    17281 gaaaaattgg cacgtcaaaa agataagact gctgaaatgg aagtgcgtat tttaacaact
    17341 attaaagaag ctttagaaaa tggtaaaccc gctcgtagta ttgactttaa tgaagaagac
    17401 caaaaatggg tgaatcaagc gcaattactg acttctaaaa aaatgcttta tatcgctaat
    17461 gttggtgaag atgaaattgg tgatgatgat aatgataaag taaaagcgat tcgtgaatat
    17521 gcagcgcaag aagactctga agtgattgtt attagtgcaa aaattgaaga agaaattgct
    17581 acattagatg atgaagataa agaaatgttc ttagaagatt taggtatcga agaaccagga
    17641 ttagatcgat taattagaac aacttatgaa ttattaggat tatcaacata ttttactgct
    17701 ggtgtgcaag aagtacgtgc ttggacattt aaacaaggta tgactgcacc tcaatgtgct
    17761 ggtatcattc atactgattt tgaacgtgga tttatccgtg ccgaagtaac aagttatgat
    17821 gactatgtac aatatggcgg cgaaagtggc gctaaagaag cgggcagaca acgattagaa
    17881 ggtaaagaat atattatgca agatggcgat atcgttcatt tcagatttaa tgtataaacg
    17941 atagagtgaa gttaattaaa tagtatatat gtagaagagg cggaatcaat tgttcgcctc
    18001 ttttaattat gcgtataatt tattaaaaga atggaatgat tttactcgcg taaataatat
    18061 cttgagtgct gaaaaattgt ttgccttcgc cagtataagc aggctctaaa acaagattag
    18121 cctttgcaca ataaagccat tcaggatgaa tgccactatt aagtatctct tggaattctt
    18181 gaaaatcttt agaccaatca atatttaaat tcattcctta ccacctcaca accattatag
    18241 aacatgcgtt cgcttttgtg aagtgtattt ttaaaaatat catagaaaat ttcaaatgaa
    18301 ttaatgtcaa aaatgatatg aagagtagtt tgaattatta ttgtaaaaag taatggcgca
    18361 tgatataatt ctttattgtg agtaatgaaa attattcctt gcttatctgt tttaagattg
    18421 ataagccgta tagaccacaa ggaggtgcaa atataaaatg agaacatatg aagttatgta
    18481 catcgtacgc ccaaacattg aggaagatgc taaaaaagcg ttagttgaac gtttcaacgg
    18541 tatcttagct actgaaggtg cagaagtttt agaagcaaaa gactggggta aacgtcgcct
    18601 agcttatgaa atcaatgatt tcaaagatgg cttctacaac atcgtacgtg ttaaatctga
    18661 taacaacaaa gctactgacg aattccaacg tctagctaaa atcagtgacg atatcattcg
    18721 ttacatggtt attcgtgaag acgaagacaa gtaataatta gagggggcgt ttaaatgcta
    18781 aatagagttg tattagtagg tcgtttaacg aaagatccgg aatacagaac cactccctca
    18841 ggtgtgagtg tagcgacatt cactcttgca gtaaatcgta cgttcacgaa tgctcaaggg
    18901 gagcgcgaag cagattttat taactgtgtt gtttttagaa gacaagcaga taatgtaaat
    18961 aactatttat ctaaaggtag tttagctggt gtagatggtc gcttacaatc ccgtaattat
    19021 gaaaatcaag aaggtcgtcg tgtgtttgtt actgaagttg tgtgtgatag cgttcaattc
    19081 cttgaaccta aaaatgcgca acaaaatggt ggccaacgtc aacaaaatga attccaagat
    19141 tacggtcaag gattcggtgg tcaacaatca ggacaaaaca attcgtacaa taattcatca
    19201 aacacgaaac aatctgataa tccatttgca aatgcaaacg gaccgattga tataagtgat
    19261 gatgacttac cattctaata aaaattaacg aaattaaagc gaaaaaatta tcaaaggagg
    19321 cacacaatca tggcaggtgg accaagaaga ggcggacgtc gtcgtaaaaa agtatgctat
    19381 ttcacagcaa atggtattac acatatcgac tacaaagaca ctgaattatt aaaacgtttt
    19441 atctcagaac gcggtaaaat tttaccacgt cgtgtaactg gtacttcagc taaatatcaa
    19501 cgtatgttga ctacagctat caaacgttct cgtcatatgg cattattacc atatgttaaa
    19561 gaagaacaat aatatttaat atattataac cctcgtaggc ataggcttat gggggatttt
    19621 tttatgtttg ggatatagaa aagggacaga aaagggcaaa aaaggatgat gtgaatgttt
    19681 tgtgttcgga atttgcacaa agatatgttt atattgcaaa aataatatga atttagatgc
    19741 ataaaaaaag aactacgcat tttaaataaa atgcatagct cttctttttc ttgcatacga
    19801 attaaaataa ctcgcgagac ctataagtct ctttcctcac tagatagttt atacttttgg
    19861 tctgttgaag tcaataattt tatctaaagc tataaaaaat cttttgatag ctaatgcatt
    19921 attataatag ctttcgtttc ttttatatcg cttttgaagt tggtccaaat cgtgatatct
    19981 tgcttggata attgcattac tacaaacttg attatgtaat tctagcgtag cgaaagtatc
    20041 tatgaaattt tttattccga acatgtttct agatatgcct atattattcc ctttttcaaa
    20101 taaatattga ggagatctac tgtcataatt tagatttgct atgatgggtt ggttatgagc
    20161 tgatttgttt cttatatttt taactaaagg cattaaaata ttagcaactc tcaattcttc
    20221 gtcattgtac ttcttgtaat agaagttgag aaacgaaacg aattgaccta gttgcatgaa
    20281 ttcaatgcaa acccatgcgg gtggattttg atagtattta ttcaacttct cgggtagttg
    20341 tcctcgttta ttcatatgct tgaatatttc gtttttattt ttgattttgg tttccataac
    20401 ttcttctggt gttcttgaat ttgtgtcaaa atttgaattg ctatatgatt tatcaataca
    20461 taagaactca tctattattt tataaccatc ttcttggtta ttttctgtta ttagttttaa
    20521 gactagacac tttaaactat gttcaatatc taaagttaaa tgcaacattg tgtatcttaa
    20581 tttcatatct atagttgcta aatctgataa ataagcaaat tctatgaaat agccgccatt
    20641 ctttttttcg aaattttttc ggaaataagc tagtttgaag aagtaattat tttttctaag
    20701 aatttcattt gctttttcgg tgtcaataat attaaaaaat atattcatct gttttaattt
    20761 cgctatttgc tcatcaaaat tgagcatagg cttaatttct gttagtatat catctttttc
    20821 caattttaac tccccaatcg ttcaaattta ttcatcatat ctttcgccat ctgattagta
    20881 acatgtgtgt atatctctag attttttata atctgaatga cctacatgct cttgcattgc
    20941 ttttaagtta attcctaatt gagcaagtgt agatatatgc gaatgatgta atgtatgcgt
    21001 cgttacaggt ttcttaatag aactaatctc agttgtctct ttgatagtgc cccctttaat
    21061 aatgttgcta attttgttcg agtcgatagg gccaccagct gcatttgtga atatgtaccc
    21121 tatattaata aacttatcat tacaagcata agtgttctta gtaagtcgat gctatggata
    21181 gtgaggccta tagccctata gctgttactt gttctagttg tctcttttac tccggatgct
    21241 cccgtcattt ttcagttatc caattaactt taccgtcgat atctagcgtt ttatcttcat
    21301 agtttatatt tactctcttt attgcaagta gctcaccgat acgcatgtca ttagcaattt
    21361 gaaactgtac catagctttt accattttat aattacgttt tgtcgttgga tatttttata
    21421 cttaattaca tagtcgaaac aatccagtaa ctccttaact tcattatctt ctaatgtgtt
    21481 actatgttta gctagtaacg catcactata gggatatcta ttttatctat tacacatata
    21541 actttgaatt gcttgctatt ttaaattaac aaattttatt ctcttagatt ttgtccaatt
    21601 atgtgtagac gatttatagt tattaaattc agagtggtag caaattaaag ttaatcaaga
    21661 gttaagatga atttaattca tgaacacgtc tattattttt ataattgtag caaataaagc
    21721 tttacatcaa ggaggtaatt aaatatgttc aaaaaatatg actcaaaaaa ttcaatcgta
    21781 ttaaaatcta ttctatcgct aggtatcatc tatgggggaa catttggaat atatccaaaa
    21841 gcagacgcgt caacacaaaa ttcctcaagt gtacaagata aacaattaca aaaagttgaa
    21901 gaagtaccaa ataattcaga aaaagctttg gttaaaaaac tttacgatag atacagcaag
    21961 gatacaataa atggaaaatc taataaatct aggaattggg tttattcaga gagaccttta
    22021 aatgaaaacc aagttcgtat acatttagaa ggaacataca cagttgctga tagagtatat
    22081 acacctaaga gaaatattac tcttaataaa gaagttgtca ctttaaagga attggatcat
    22141 atcataagat ttgctcatat ttcttatggc ttatatatgg gagaacattt gcctaaaggt
    22201 aacatcgtca taaatacaaa agatggcggt aaatatacat tagagtcgca taaagagcta
    22261 caaaaagata gggaaaatgt aaaaattaat acagccgata taaaaaatgt aactttcaaa
    22321 cttgtgaaaa gtgttaatga cattgaacaa gtttgaaatt aagttgaatt agtatataca
    22381 gtgttttatt gctaatactt tgaaagttag gtatctaaag gtgcctagct ttctttgtta
    22441 tgattagcac tatcatatag aaaattcttt aatgacgttt atgccgaaat ctacaaatat
    22501 aatttctctt ttatggttaa tcaatatgtt tttacattcg acaaccttaa aattaacttc
    22561 tttcaattcc ataataaagt ctctataaaa taacttagtt taaaaacgat tcgtatcttt
    22621 caggttcaaa taccatcatt ttctcctaat acttacactt taattacaat tatgtaagtt
    22681 gttttcagag gcatcctatt ttatattttg tcagaggtga tcttttgaaa acgattttaa
    22741 aaacaataac atatcttgca cttactatca ttggcgctta tgctgcttta ttcattttaa
    22801 aaacaataga ctctcatggt ataacagatc aatttaaccc attagtaaag gaagatgatt
    22861 cttatgttaa aacgacagag gtgtctacta gaatggatga tcaactccga agttatactc
    22921 aaagtgcttt taataaagaa gggaaagaga cgcaattaat gtatactgct acatttgatg
    22981 ttaaaccgca tagatacttg aaaattacac ataaaggtca tcatgtagaa acttttgaag
    23041 aagttgaaaa ggaagaagta ccaaagaaag cattagacaa actgagtcga taatagcatg
    23101 cttatattac atggttcatt tataaaagga gtacgaacga aagtaacgca tgacgattaa
    23161 tttaaaaata tttgtaataa ttatgaataa aattaaaaac aaggggtaat acaatctata
    23221 tagcatataa gcttttgtta tgagtttcaa aaataggaag agagagtgat attatgaaat
    23281 taaaatcatt agcagtgtta tcaatgtcag cggtggtgct tactgcatgt ggcaatgata
    23341 ctccaaaaga tgaaacaaaa tcaacagagt caaatactaa tcaagacact aatacaacaa
    23401 aagatgttat tgctttaaaa gatgttaaaa caagcccaga agatgctgtg aaaaaagctg
    23461 aagaaacata caaaggccaa aagttgaaag gaatttcatt tgaaaattct aatggtgaat
    23521 gggcttataa agtgacgcaa caaaaatctg gtgaagagtc agaagtactt gttgctgata
    23581 aaaataaaaa agtgattaac aaaaagactg aaaaagaaga tacagtgaat gaaaatgata
    23641 actttaaata tagcgatgct atagattaca aaaaagccat taaagaagga caaaaagaat
    23701 ttgatggtga tattaaagaa tggtcacttg aaaaagatga tggcaaactt gtttacaata
    23761 tcgatttgaa aaaaggtaat aaaaaacaag aagttactgt tgatgctaag aacggtaaag
    23821 tattaaagag tgagcaagat cactaaaaag attcgttgca ttaacgcttg taaaatgtct
    23881 taattcatat taattaataa aataccctca caatacttat gcataagtcc tgtttgacgt
    23941 gaaaggatat atgccaaacg tgttgtgagg gtttaattgc atctatttat ccatgcgaat
    24001 atcgacttct tctaaatgtt tctgatattc tttaacctta ctttctaaaa acatttcata
    24061 tggtgcatca aagaagtcag ctaaatgcat ggcatcttta aatttaggtt catgatgatg
    24121 attctcccat tcccaaattt gatgtgcttc atatttagta ccatatttct catttaattg
    24181 ctgtgctaat tcgtcaattt ctaaattatg tttagttcgt aagttatata aaatatgcat
    24241 attcattgtt cattaacctc gcttcttgat ttaaaattca aagatgctct aataatatgt
    24301 gtatattcta gtcctataac ataaaataat caagctatga attcggcaga aaaaataatg
    24361 taaatgtata tgatattgcc agtacagaga ctttaaataa cgatagctac attgaataaa
    24421 attgatattc aattactact tttaaaaata tttggataaa aataatttga atttgtttta
    24481 gaattgtaaa taaggggtac tacttagaat aacgcaaaat aaatagaaaa aggagactga
    24541 aaattatgtt tggatttatt ggaatgttaa ttgtcggtgg cttaattgga tgggctgctg
    24601 gtgctattat gggtaaagat atcccaggtg gtattttagg caatattatc gcaggtatta
    24661 ttggatcatg ggtaggtggc aaactattcg gacaatgggg tcctgaatta ggaagtattt
    24721 acatcttgcc agcattaatt ggttcaatta tcttaattgc aatcgtaacg ttaattttaa
    24781 gagctatgcg taaataataa aaaaattcaa ttggaaattg aaataaagat ggcattcagt
    24841 tgtcatcttt tttatttgcg tgaaaatggt caatatgtat tataatattt gaaaatgtag
    24901 ataagatagg ataaaaaagt aataaattaa tatgaggaaa ttttggaaat aaaaaagcga
    24961 gtatttgaca aataaaaaaa ggatgacgtt gggttgcgcc atccaggagt attatgttca
    25021 tgaatataaa agactgtgta gtcatttaag aactacaccc atatagtaac acatcacttg
    25081 attaaagaca atactatttt tagagtcatt aatttaaaat atgaattaat tcttttttta
    25141 gaatagaata ttaagattga tatcgaatta gtagtcaaag tgttatggta gatatgaaat
    25201 acataaggta aggagtaata ttatgacgat ttatttagtt agacatggcg aatcaaaatc
    25261 gaattatgat aataaacatt ttcgatctta tttttgtgga caattagatg tgccgttaac
    25321 ggatactggc acaaaaagtg cagacgattt atgtgattat tttaaagaga aacagattaa
    25381 acatgtatat gtttcagact tattaagaac acagcaaacg tttgaacata tttttccata
    25441 tgacattgca tcaacgacta cccctctatt aagagaacgt tcacttggcg tatttgaggg
    25501 tgaatataaa gatgaaatca gtgcgaatcc gaaatatgaa aaatatttca atgatccaaa
    25561 ctttaaagac tttcgtcata gtttttcaca aaaagcgcct gaaggagaaa gttatgaaga
    25621 tgtatatcaa cgcgtagaac attttatgaa tcatgttgtc aatgaagata cacaaaaaga
    25681 tgatattgtc attgttgcac atcaagttgt cattcgttgt ttgatggttt attttaacaa
    25741 agtttcaagg gaagaagctg tggatttaaa ggtagaaaat tgcaaaccat atatcattga
    25801 atagaagtat agaggttctg aaaagctgtg ttttatagtg gccttcagaa ccttttggca
    25861 tttggaattt aatgggaatc attcttatca acagacttac caccaatagc taatgtgata
    25921 attgcgctta tgaacaaaat aattgaagtt ataaatgacg ctaatgtaaa aggtgtaaat
    25981 tgtgtgagcg ataatatcaa tgataaaaag aaccaagaga tagcccctag aaattgtaaa
    26041 atagcagaag aaattctaaa actcatataa tcatatatag ctaaaacaag tgaaggtaat
    26101 gcgactaata ccattaaaat gatgttgagg gtgaataaat atggctgttc aaaagttact
    26161 gtgtttggtc ctgttggatg cattgctgct aagaacaagc aaacaatcgt cgataaaatt
    26221 gctaaaataa taaaagtaat tcgaactttc atcatgacca tcctttgttt atagagtcaa
    26281 tataagtatg aaatatgtta gggatatagt caaatgcgtc aactaatggg tgattttggc
    26341 atagatagag aatttaaggc aattaaaaaa gcatcaaaca gtaatatgct gcttgatgca
    26401 aaatggtaag gtattggtta aattgtattg tctcaaagat tagtcacttt taaagataaa
    26461 gaattgtcat gaattaaagc tcatgtaatg atgtgttaca tgtcgcaatg atggctttca
    26521 gttatttatc gataacatca ctcttgatac ctttagattt taagaaatct ttaattttat
    26581 cttgttgctt tttattaaca tcaccggcat attttgttgg cacgtcgact acattgattt
    26641 tattttgcgg ttgatagcta agcttttcaa tatcttcatc aacattggcg attgtactat
    26701 ttaaagcttt gaagtaattc atcattaatt caacgggttt cttatattct ttaggaatat
    26761 tgttttcagt gacaaatttc ttgaaatgca aatcgttttt aacagctaag ttagataagt
    26821 ggctaagtgt ttctgcttgt ttttcagtca cttttgtttg actgtcaatt tgtttatcta
    26881 gtttatgttg cataatatat ttgttatcaa gtatatcgct atttacagac aaatactttt
    26941 ctatagcttg cttcatctct gcatcactaa tatcactatt tttcttatct gagttaaaga
    27001 tatcttttgt ttctaatttt ttagcgcttt taggtgcatg gatgccagta cttgtgtgat
    27061 gatcttcgtt atcagattga tcggacgcgc aacctgtaag aattaatgca gatgctaaaa
    27121 atgtacttag tagtaatctc tttttcataa tgtaatataa ctccttagtt tatctttaat
    27181 tgaaaaaata tgtattcatg tttaatagag taacattgaa ttagtttgga atgtcacgat
    27241 gaccttgcaa tgaccataga cgcaaatgat tacgtgcatg agttgctttt tctatcaata
    27301 atgcgtcgtt ttgaacaatg ttaaggatag cattatctat aaataactgc ataattggtt
    27361 gtatcaattt agatgtaggt atcgtacgta aaagcataat gatttcgttc acatactttt
    27421 ccttctcaat atcattttca tattgatttg tttgcgagag gtacatactt taagcattat
    27481 cgcacatctc gttgtatata ttaagtttat cataacatga ttttatgtcg ggataaaaaa
    27541 ataacagcat cttaacaaat gtaagatact gtcagtgaaa ctgtgaaaac tttagtttct
    27601 gataatatag tcaaaggcat ttaatgctgc atttgcacca gcgcccattg aaatgataat
    27661 ttgtttgttc ttctgatctg tgacatcgcc agcagcaaat attccaggta cgttagtatt
    27721 attgttacga tcaataacaa tttcaccacg ttcgtttaat tcaacagcat cttttaacca
    27781 tgatgtgttt ggaagtaaac caatttgaac aaagatacca tctaagttaa gtagatgttc
    27841 ttcgccggtg ctcatgtctt cgtaacgtat acctgtaaca tggtcttctc cgacaacttc
    27901 agtagttttg gcatttgttt tgatatcaac atttgataaa gaacgtaaac gatcttgtaa
    27961 cacgttgtct gcttttaatt cgctagcgaa ttcgaataat gtaacatgat taacgatacc
    28021 agcaaggtca attgctgctt caaccccaga gttaccgcca ccgataactg ctacgtcttt
    28081 attttcgaat agaggtccgt cacagtgagg gcagaatgca acacctttat taatcaattg
    28141 ctcttcacct ggaatgttta gcttacgcca acctgcacca gtagcaataa tgactgtttt
    28201 actttctaag acagcaccgt tttctaacgt aactttaatt gcttcgtcag tcttttcgat
    28261 atctgtagca cgtatacctg tcattgcatc aatgtcatat tgatcaatgt gcgctgctaa
    28321 gttagaagaa aattcagaac cagttgtttc tttaacagta atgaagttct caataccagc
    28381 agtatcatta acttggccac cgatacgatc agcaactata ccagtacgta aacctttacg
    28441 tgctgtgtaa atcgctgcac taccactagc aggaccacca ccaacgatta agacatcata
    28501 aggttcttta ttttcaaact cagatgcatc tgccgtactg cctagtttcg aaagaatatc
    28561 ttggattgtc atacgaccat tgccaaattc ttcgccgttt aaaaagacag cagggactgc
    28621 catgatgttt tcagattctt cacggaacac tgcaccatca atcatagaat gcgtgatgtt
    28681 agggttgatc acactcatta agttaagtgc ttgaacgaca tcaggacatt tttgacacgt
    28741 taaactaatg aatgtttcaa aatggaatga accttctaag tttttaattt ggtcaataat
    28801 tgactgtttt tctttaggtg cacgaccact aacctgtaaa attgctaaaa caagtgagtt
    28861 aaactcgtga cctaatggaa ttcctgcaaa tgttacacct gtttcttcgc caggacggtt
    28921 gactgagaaa cttggtgtac gttttaaaga cttttcagaa agtgaaagtc taggtgacat
    28981 atcagaaatt tctgttaata attctttgag ttccttagat ttttcatctg aaccaaggct
    29041 ggcaacgaat tcaacgttgc cctccattag ttctaataat tgtttaagtt gttgttttaa
    29101 atcagcatta agcatggttg taatgcctcc ttagatttta cctactaaat ctaaaccagg
    29161 ttgcaatgtt ttagcgcctt cttcccattt agctgggcat acttcgccag ggtttttacg
    29221 aacatattga gctgctttga ttttgtgagc taatgtacta gcgtcacggc caattccgtc
    29281 agcgttaatt tcagatgctt gtacaacacc gtctgggtcg ataatgaatg taccacgttg
    29341 agctaaacca gtagcttcat ctaatacatc aaaattacga gtgattgttt gtgatgggtc
    29401 accaatcata gtgtaagtga ttttgctaat tgcatctgaa tggtcatgcc atgctttgtg
    29461 tacgaagtga gtatcagttg atactgagaa tacatttacg cctaattttt gtaattcttc
    29521 atattggttt tgtaagtctt ctaattcagt tggacaaacg aatgagaagt cagcaggata
    29581 gaagcatact acgctccaag aaccttttaa atcttcttgt gtaacttctt taaattgatc
    29641 tttttttgga tcgaaagctt gcgctgtaaa tggtaagatt tctttgttaa ttaatgacat
    29701 aaatatcttc ctcctaagaa tttaagtatg aattagaact atcaattgat tgtgcttaat
    29761 tataataatt ctaatctctt agttagcatt attacatttt gatccagaat agtcaactgg
    29821 ataactttgt aaagtgaacg attactttta aaataaagaa agataatata aagtgctttg
    29881 ataatggatt ttgtagttga tgatttaaaa ggttgtgtct atatttaata tcttgatttt
    29941 aatgtaaaaa atgtaaaaaa agaagatttg tattctcaac taagtcaacc ttattgataa
    30001 tggtgtgaga atatttgttc gagatggatg aaggtgatta gtgagaaact ggaattttca
    30061 agtacgagac aatattttta aaagttcaat tattaactta taagcaaata attgctataa
    30121 aaaagtttgg acgtgtacaa ttgcaatatg aagattttta attaactgta aagtatcgag
    30181 gagtgggtaa cgtgtcagat tatgtatata atcttgtgaa aaagcatcat tctgttagaa
    30241 aatttaagaa taaaccttta agtgaagacg ttgttaagaa attggtagaa gctggacaaa
    30301 gcgcttcgac gtcaagtttc ctacaagcat actcaattat tggtatcgac gatgagaaga
    30361 ttaaagaaaa tttacgagaa gtttctggac aaccttatgt tgtagaaaat ggctatttat
    30421 tcgtctttgt tattgattat tatcgtcatc atttagttga tcaacatgct gaaactgata
    30481 tggaaaatgc atatggttca acggaaggtt tgctagtagg tgcaatcgat gcagcattag
    30541 ttgctgaaaa tattgcggta actgctgaag atatggggta tggcattgtc tttttaggat
    30601 cattaagaaa tgatgttgaa cgagttcgag aaattttaga cttacctgac tatgtcttcc
    30661 cggtatttgg tatggcagta ggggaacccg cagatgatga aaatggtgca gccaagccac
    30721 gcttaccatt tgaccatgtc ttccatcata ataagtatca tgctgataag gaaacacagt
    30781 atgcacaaat ggcagattac gaccagacaa tcagcgagta ctatgatcaa cgtacaaacg
    30841 ggaatcgcaa agaaacatgg tcgcagcaaa ttgagatgtt cctaggaaac aaagcaagat
    30901 tagatatgtt agaacaattg caaaaatcag gcttaataca gcgatagcaa gataccaaaa
    30961 t
//
LOCUS       DAOBBH010000011        30720 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.011, whole genome shotgun sequence.
ACCESSION   DAOBBH010000011 DAOBBH010000000
VERSION     DAOBBH010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 30720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30720
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.011"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(123..962)
                     /gene="noc"
                     /locus_tag="P6L01_000335"
     CDS             complement(123..962)
                     /gene="noc"
                     /locus_tag="P6L01_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HDP3029434.1"
                     /translation="MKKPFSKLFGLKNKDDIIGHIEEDRNSNVESIQIERIVPNRYQP
                     RQVFEPNKIKELAESIHEHGLLQPIVVRPIEEDMFEIIAGERRFRAIQSLNLPQADVI
                     IRDMDDEETAVVALIENIQRENLSVVEEAEAYKKLLEIGDTTQSELAKSLGKSQSFIA
                     NKLRLLKLAPKVLLRLREGKITERHARAVLSLSDSEQEALIEQVIAQKLNVKQTEDRV
                     RQKTGPEKVKAQNLRFAQDVTQARDEVGKSIQAIQQTGLHVEHKDKDHEDYYEIKIRI
                     YKR"
     gene            complement(1005..1724)
                     /gene="rsmG"
                     /locus_tag="P6L01_000336"
     CDS             complement(1005..1724)
                     /gene="rsmG"
                     /locus_tag="P6L01_000336"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDP3029435.1"
                     /translation="MTVEWLAEQLKEHNIELTETQKQQFQTYYRLLVEWNEKMNLTSI
                     TDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSL
                     NKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
                     LIKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFKLPEDAGERQMFIIDKKR
                     QTPKKYPRKPGTPNKTPLLEK"
     gene            complement(1724..3601)
                     /gene="mnmG"
                     /locus_tag="P6L01_000337"
     CDS             complement(1724..3601)
                     /gene="mnmG"
                     /locus_tag="P6L01_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDP3029436.1"
                     /translation="MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMP
                     CNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLY
                     QQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
                     IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPG
                     DDVGRAFSFETTEYILDQLPCWLTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPS
                     IEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMM
                     RAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKV
                     LNTGEKILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGY
                     ELGMISEERYARFNEKRQQIDAEIKRLSDIRIKPNEHTQAIIEQHGGSRLKDGILAID
                     LLRRPEMTYDIILEILEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKRMEEKKI
                     PEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVS
                     D"
     gene            complement(3668..5047)
                     /gene="mnmE"
                     /locus_tag="P6L01_000338"
     CDS             complement(3668..5047)
                     /gene="mnmE"
                     /locus_tag="P6L01_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDP3029437.1"
                     /translation="MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLND
                     VPSHTINYGHIIDPESKEVVEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELT
                     MTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
                     KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIM
                     REGLSTVIVGKPNVGKSSMLNNLIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDT
                     AGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIV
                     NKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYV
                     SNSRHISLLKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQ
                     LFSQFCLGK"
     gene            complement(5204..5557)
                     /gene="rnpA"
                     /locus_tag="P6L01_000339"
     CDS             complement(5204..5557)
                     /gene="rnpA"
                     /locus_tag="P6L01_000339"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDP3029438.1"
                     /translation="MLLEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFR
                     LGISVSKKLGNAVLRNKIKRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSL
                     EHVLKIAKVFNKKIK"
     gene            complement(5678..5815)
                     /gene="rpmH"
                     /locus_tag="P6L01_000340"
     CDS             complement(5678..5815)
                     /gene="rpmH"
                     /locus_tag="P6L01_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDP3029439.1"
                     /translation="MVKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS
                     A"
     gene            6546..7907
                     /gene="dnaA"
                     /locus_tag="P6L01_000341"
     CDS             6546..7907
                     /gene="dnaA"
                     /locus_tag="P6L01_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDP3029440.1"
                     /translation="MSEKEIWEKVLEIAQEKLSAVSYSTFLKDTELYTIKDGEAIVLS
                     SIPFNANWLNQQYAEIIQAILFDVVGYEVKPHFITTEELANYSNNETATPKETTKPST
                     ETTEDNHVLGREQFNAHNTFDTFVIGPGNRFPHAASLAVAEAPAKAYNPLFIYGGVGL
                     GKTHLMHAIGHHVLDNNPDAKVIYTSSEKFTNEFIKSIRDNEGEAFRERYRNIDVLLI
                     DDIQFIQNKVQTQEEFFYTFNELHQNNKQIVISSDRPPKEIAQLEDRLRSRFEWGLIV
                     DITPPDYETRMAILQKKIEEEKLDIPPEALNYIANQIQSNIRELEGALTRLLAYSQLL
                     GKPITTELTAEALKDIIQAPKSKKITIQDIQKIVGQYYNVRIEDFSAKKRTKSIAYPR
                     QIAMYLSRELTDFSLPKIGEEFGGRDHTTVIHAHEKISKDLKEDPIFKQEVENLEKEI
                     RNV"
     gene            8185..9318
                     /gene="dnaN"
                     /locus_tag="P6L01_000342"
     CDS             8185..9318
                     /gene="dnaN"
                     /locus_tag="P6L01_000342"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDP3029441.1"
                     /translation="MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVI
                     LTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQ
                     FQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETR
                     PVLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDN
                     EEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAID
                     RASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYM
                     MDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY"
     gene            9699..9944
                     /gene="yaaA"
                     /locus_tag="P6L01_000343"
     CDS             9699..9944
                     /gene="yaaA"
                     /locus_tag="P6L01_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDP3029442.1"
                     /translation="MIILVQEVVVEGDINLGQFLKTEGIIESGGQAKWFLQDVEVLIN
                     GVRETRRGKKLEHQDRIDIPELPEDAGSFLIIHQGEQ"
     gene            9941..11053
                     /gene="recF"
                     /locus_tag="P6L01_000344"
     CDS             9941..11053
                     /gene="recF"
                     /locus_tag="P6L01_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDP3029443.1"
                     /translation="MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIY
                     TLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGTMPLTMFITKKGKQVKVNHLEQS
                     RLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
                     NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDK
                     EALSLNYLPSLKFDYAQNEAARLEEIMSILSDNMQREKERGISLFGPHRDDISFDVNG
                     MDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLSELDDSRQTHLLSTI
                     QHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK"
     gene            11063..12997
                     /gene="gyrB"
                     /locus_tag="P6L01_000345"
     CDS             11063..12997
                     /gene="gyrB"
                     /locus_tag="P6L01_000345"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDP3029444.1"
                     /translation="MVTALSDVNNTDNYGAGQIQVLEGLEAVRKRPGMYIGSTSERGL
                     HHLVWEIVDNSIDEALAGYANKIEVVIEKDNWIKVTDNGRGIPVDIQEKMGRPAVEVI
                     LTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSQDLEVYVHRNETIYHQAYKKGVPQF
                     DLKEVGTTDKTGTVIRFKADGEIFTETTVYNYETLQQRIRELAFLNKGIQITLRDERD
                     EENVREDSYHYEGGIKSYVELLNENKESIHDEPIYIHQSKDDIEVEIAIQYNSGYATN
                     LLTYANNIHTYEGGTHEDGFKRALTRVLNSYGLSSKIMKEEKDRLSGEDTREGMTAII
                     SIKHGDPQFEGQTKTKLGNSEVRQVVDKLFSEHFERFLYENPQVARTVVEKGIMAARA
                     RVAAKKAREVTRRKSALDVASLPGKLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSR
                     TQAILPLRGKILNVEKARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTD
                     ADVDGAHIRTLLLTFFYRFMRPLIEAGYVYIAQPPLYKLTQGKQKYYVYNDRELDKLK
                     SELNPTPKWSIARYKGLGEMNADQLWETTMNPEHRALLQVKLEDAIEADQTFEMLMGD
                     VVENRRQFIEDNAVYANLDF"
     gene            13034..15703
                     /gene="gyrA"
                     /locus_tag="P6L01_000346"
     CDS             13034..15703
                     /gene="gyrA"
                     /locus_tag="P6L01_000346"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDP3029445.1"
                     /translation="MAELPQSRINERNITSEMRESFLDYAMSVIVARALPDVRDGLKP
                     VHRRILYGLNEQGMTPDKSYKKSARIVGDVMGKYHPHGDSSIYEAMVRMAQDFSYRYP
                     LVDGQGNFGSMDGDGAAAMRYTEARMTKITLELLRDINKDTIDFIDNYDGNEREPSVL
                     PARFPNLLANGASGIAVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDF
                     PTAGLILGKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARMIEK
                     IAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQTPLQTSFGVN
                     MIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRKAKDRAHILEGLRIALDHID
                     EIISTIRESDTDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLN
                     YISELETILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFEDLEDEDLIPEEQIV
                     ITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFFTNKGR
                     VYKLKGYEVPELSRQSKGIPVVNAIELENDEVISTMIAVKDLESEDNFLVFATKRGVV
                     KRSALSNFSRINRNGKIAISFREDDELIAVRLTSGQEDILIGTSHASLIRFPESTLRP
                     LGRTATGVKGITLREGDEVVGLDVAHANSVDEVLVVTENGYGKRTPVNDYRLSNRGGK
                     GIKTATITERNGNVVCITTVTGEEDLMIVTNAGVIIRLDVADISQNGRAAQGVRLIRL
                     GDDQFVSTVAKVKEDAEDETNEDEQSTSTVSEDGTEQQREAVVNDETPGNAIHTEVID
                     SEENDEDGRIEVRQDFMDRVEEDIQQSSDEDEE"
     gene            complement(15791..16603)
                     /locus_tag="P6L01_000347"
     CDS             complement(15791..16603)
                     /locus_tag="P6L01_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDP3029446.1"
                     /translation="METLNSINIPKRKEDSHKGDYGKILLIGGSANLGGAIMLAARAC
                     VFSGSGLITVATHPTNHSALHSRCPEAMVIDINDTKMLTKMIEMTDSILIGPGLGVDF
                     KGNNAITFLLQNIQPHQNLIVDGDAITIFSKLKPQLPTCRVIFTPHLKEWERLSGIPI
                     EEQTYERNREAVDRLGATVVLKKHGTEIFFKDEDFKLTIGSPAMATGGMGDTLAGMIT
                     SFVGQFDNLKEAVMSATYTHSFIGENLAKDMYVVPPSRLINEIPYAMKQLES"
     gene            16929..18443
                     /gene="hutH"
                     /locus_tag="P6L01_000348"
     CDS             16929..18443
                     /gene="hutH"
                     /locus_tag="P6L01_000348"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HDP3029447.1"
                     /translation="MTLYLDGETLTIEDIKSFLQQQSKIEIIDDALERVKKSRAVVER
                     IIENEETVYGITTGFGLFSDVRIDPTQYNELQVNLIRSHACGLGEPFSKEVALVMMIL
                     RLNTLLKGHSGATLELVRQLQFFINERIIPIIPQQGSLGASGDLAPLSHLALALIGEG
                     KVLYRGEEKDSDDVLRELNRQPLNLQAKEGLALINGTQAMTAQGVISYIEAEDLGYQS
                     EWIAALTHQSLNGIIDAYRHDVHSVRNFQEQINVAARMRDWLEGSTLTTRQAEIRVQD
                     AYTLRCIPQIHGASFQVFNYVKQQLEFEMNAANDNPLIFEEANETFVISGGNFHGQPI
                     AFALDHLKLGVSELANVSERRLERLVNPQLNGDLPAFLSPEPGLQSGAMIMQYAAASL
                     VSENKTLAHPASVDSITSSANQEDHVSMGTTAARHGYQIIENARRVLAIECVIALQAA
                     ELKGVEGLSPKTRRKYEEFRSIVPSITHDRQFHKDIEAVAQYLKQSIYQTTACH"
     misc_binding    18516..18732
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            18822..20108
                     /gene="serS"
                     /locus_tag="P6L01_000349"
     CDS             18822..20108
                     /gene="serS"
                     /locus_tag="P6L01_000349"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDP3029448.1"
                     /translation="MLDIRLFRNEPDTVKSKIELRGDDPKVVDEILELDEQRRKLISA
                     TEEMKARRNKVSEEIALKKRNKENADDVIAEMRTLGDDIKEKDSQLNEIDNKMTGILC
                     RIPNLISDDVPQGESDEDNVEVKKWGTPREFSFEPKAHWDIVEELKMADFDRAAKVSG
                     ARFVYLTNEGAQLERALMNYMITKHTTQHGYTEMMVPQLVNADTMYGTGQLPKFEEDL
                     FKVEKEGLYTIPTAEVPLTNFYRNEIIQPGVLPEKFTGQSACFRSEAGSAGRDTRGLI
                     RLHQFDKVEMVRFEQPEDSWNALEEMTTNAEAILEELGLPYRRVILCTGDIGFSASKT
                     YDIEVWLPSYNDYKEISSCSNCTDFQARRANIRFKRDKAAKPELAHTLNGSGLAVGRT
                     FAAIVENYQNEDGTVTIPEALVPFMGGKTQISKPVK"
     gene            20759..21454
                     /locus_tag="P6L01_000350"
     CDS             20759..21454
                     /locus_tag="P6L01_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDP3029449.1"
                     /translation="MATHLSFRQGVQECIPTLLGYAGVGISFGIVASSQNFSILEIIL
                     LCLVIYAGAAQFIMCALFIAGTPISAIVLTVFIVNSRMFLLSMSLAPNFKTYGFWNRV
                     GLGSLVTDETFGVAITPYLKGEAINDRWMHGLNITAYLFWAISCVAGALFGEYISNPQ
                     TLGLDFAITAMFIFLAIAQFESITKSRLRIYIVLIIAVIVMMLSLSMFMPSYLAILIA
                     ATISAALGVMMER"
     gene            21451..21780
                     /locus_tag="P6L01_000351"
     CDS             21451..21780
                     /locus_tag="P6L01_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDP3029450.1"
                     /translation="MITHMNMLILILLCGIVTLLIRIIPFIMISKVQLPDVVVRWLSF
                     IPITLFTALVIDSIIQQTPHGEGYTLNIPYIIALIPTVILSIITRSLTITIISGIVIM
                     ATLRFFF"
     regulatory      21985..22083
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            22143..23111
                     /locus_tag="P6L01_000352"
     CDS             22143..23111
                     /locus_tag="P6L01_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDP3029451.1"
                     /translation="MTNYTVDTLNLGKFITESGEVIDNLRLRYEHVGYHGQPLVVVCH
                     ALTGNHLTYGTDDYPGWWREIIDGGYIPIHDYQFLTFDVIGSPFGSSSPLNDPHFPKK
                     LTLRDIVRANERGIQALGYDKINILIGGSLGGMQAMELLYNQQFEVDKAIILAATSRT
                     SSYSRAFNEIARQAIHLGGKEGLSIARQLGFLTYRSSKSYDERFTPDEVVAYQQHQGN
                     KFKEHFDLNCYLTLLDVLDSHNIDRGRTDVTHIFKNLETKVLTMGFIDDLLYPDDQVR
                     ALGERFKYHRHFFVPDNVGHDGFLLNFSTWAPNLYHFLNLKHFKRK"
     gene            23404..24342
                     /locus_tag="P6L01_000353"
     CDS             23404..24342
                     /locus_tag="P6L01_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HDP3029452.1"
                     /translation="MGVAYVFSKIQPKATIIATITLVFVALALYLVPGLGLIFALFAT
                     IPGIVLWNKSIQSFGVSALITVIITTVLGNTFVLSAIILVLIASLIIGQLLKERTSKE
                     RMLYVTTVAMSLISLIAFMLLQTFGRIPPSASIVKPFKQTLHEAITMSGADANMTQIL
                     EEGFRQATVQLPGFIIIITFLIVLINLIVTFPVLRKFKIATPVFKPLFAWQMSGILLW
                     IYIIVIICLLFTGQPSVFQSILLNFQLVLSLVMYIQGLSVIHFFGKAKGLPNAVTILL
                     LIIGTILTPTTHIVGLLGVIDLSLNLKRIMKNNSKK"
     gene            24357..26324
                     /gene="gdpP"
                     /locus_tag="P6L01_000354"
     CDS             24357..26324
                     /gene="gdpP"
                     /locus_tag="P6L01_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP phosphodiesterase GdpP"
                     /protein_id="HDP3029453.1"
                     /translation="MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLV
                     VMIIVSIILFRQALMKMDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQF
                     MTNHMEANVISESVNEVFPNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITE
                     QVQTNELYENSKPIIATLFLDNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFK
                     RYSSDQFVAYLNQKILADLEESKFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLG
                     ELSQSGLDLALGRGGDQVAIKSINGNVRFYGGKTDPMEKRTRVRARVISHALKDILAE
                     GDKVIIMGHKRPDLDAIGAAIGVSRFAMMNNLEAYIVLNETDIDPTLRRVMNEIDKKP
                     ELRERFITSDDAWDMMTSKTTVVIVDTHKPELVLDENVLNKANRKVVIDHHRRGESFI
                     SNPLLIYMEPYASSTAELVTELLEYQPTEQRLTRLESTVMYAGIIVDTRNFTLRTGSR
                     TFDAASYLRAHGADTILTQHFLKDDVDTYINRSELIRTVKVEDNGIAIAHGSDDKIYH
                     PVTVAQAADELLSLEGIEASYVVARREDNLIGISARSLGSVNVQLTMEALGGGGHLTN
                     AATQLKGVTVEEAIAQLQQAITEQLSRSEDA"
     gene            26321..26767
                     /gene="rplI"
                     /locus_tag="P6L01_000355"
     CDS             26321..26767
                     /gene="rplI"
                     /locus_tag="P6L01_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDP3029454.1"
                     /translation="MKVIFTQDVKGKGKKGEVKEVPVGYANNFLLKKNYAVEATPGNL
                     KQLELQKKRAKQERQQEIEDAKALKETLSNIEVEVSAKTGEGGKLFGSVSTKQIAEAL
                     KAQHDIKIDKRKMDLPNGIHSLGYTNVPVKLDKEVEGTIRVHTVEQ"
     gene            26799..28199
                     /gene="dnaB"
                     /locus_tag="P6L01_000356"
     CDS             26799..28199
                     /gene="dnaB"
                     /locus_tag="P6L01_000356"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDP3029455.1"
                     /translation="MDRMYEQNQMPHNNEAEQSVLGSIIIDPELINTTQEVLLPESFY
                     RGAHQHIFRAMMHLNEDNKEIDVVTLMDQLSTEGTLNEAGGPQYLAELSTNVPTTRNV
                     QYYTDIVSKHALKRRLIQTADSIANDGYNDELELDAILSDAERRILELSSSRESDGFK
                     DIRDVLGQVYETAEELDQNSGQTPGIPTGYRDLDQMTAGFNRNDLIILAARPSVGKTA
                     FALNIAQKVATHEDMYTVGIFSLEMGADQLATRMICSSGNVDSNRLRTGTMTEEDWSR
                     FTIAVGKLSRTKIFIDDTPGIRINDLRSKCRRLKQEHGLDMIVIDYLQLIQGSGSRAS
                     DNRQQEVSEISRTLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDA
                     DIVAFLYRDDYYNRGGDEDDDDDGGFEPQTNDENGEIEIIIAKQRNGPTGTVKLHFMK
                     QYNKFTDIDYAHADMM"
     gene            28477..29760
                     /locus_tag="P6L01_000357"
     CDS             28477..29760
                     /locus_tag="P6L01_000357"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDP3029456.1"
                     /translation="MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQ
                     FGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRA
                     QVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
                     KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVL
                     FEGAQGVMLDIDHGTYPFVTSSNPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGP
                     FPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVL
                     TGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTSVRTLEELPEN
                     ARKYLERISELCNVQISIFSVGPDREQTNLLKELW"
     gene            30189..30263
                     /locus_tag="P6L01_000358"
     tRNA            30189..30263
                     /locus_tag="P6L01_000358"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30222..30224,aa:Glu,seq:ttc)
     gene            30271..30343
                     /locus_tag="P6L01_000359"
     tRNA            30271..30343
                     /locus_tag="P6L01_000359"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30304..30306,aa:Asp,seq:gtc)
BASE COUNT        10391 a         4942 c         5608 g         9779 t
ORIGIN      
        1 ccagcctgat caacatataa atataaattt ttatgtttca cgtaaaacaa aaaaagacaa
       61 agctgttatg atcttagctt tgtcttttat gtgttagtca tcatgtatac gacatcctac
      121 tactaacgtt tatatattcg aatttttatt tcataataat cttcatgatc tttgtcttta
      181 tgctcaacat gtagtcctgt ttgttgaatc gcttggatac ttttgcctac ctcatctcgt
      241 gcttgagtga catcttgtgc aaagcgaagg ttttgtgctt tgactttttc gggccccgtt
      301 ttttggcgta ctctatcttc agtctgtttc acatttagtt tttgtgcaat gacttgctca
      361 atcaacgctt cttgttcact atcagacaat gataataccg ctctcgcatg gcgttcagta
      421 attttacctt ctcttaagcg aagtagtact ttcggcgcca acttcaataa acgcaactta
      481 tttgcaataa agctttgact tttacctaaa ctttttgcca attcactttg cgttgtatca
      541 ccaatttcca ataatttctt ataggcttcc gcttcttcaa caacagacaa attttctctt
      601 tgaatattct caattaatgc aacaacagcc gtctcttcat catccatatc acgaataata
      661 acgtctgctt gaggtaaatt tagtgattgt attgctctaa atcggcgctc tccagcaata
      721 atttcaaaca tatcttcttc aatcggtctt acaacaatag gttgtagtaa accatgttca
      781 tgtattgatt cagcaagttc tttaatttta tttggttcaa acacctgtct tggttgataa
      841 cggttgggaa cgatacgttc aatttgaatg gattcaacat tactattgcg atcttcctca
      901 atatgtccaa tgatgtcatc tttgtttttc aaaccaaata attttgaaaa aggttttttc
      961 attatccatt cgctcctttt atgttagttg taaaggatta tgcattattt ttcaagtaaa
     1021 ggagtcttat taggcgtccc tggttttctt gggtactttt tcggcgtctg tcttttttta
     1081 tcaataatga acatctggcg ctctccagca tcttctggca atttaaaggt atgtgtttct
     1141 gtaacgttac cacctaacac actaattgca aattttgctt cttctaattc ttcttcacct
     1201 tttgaagatt ttaatgcaac aaactgtcca ccttttttaa ttagcggtaa acacaattca
     1261 ctcaacacgg ataatctagc tactgctctt gcagtaacaa catcataaga ctccctgtag
     1321 acacccttac caaatgtttc tgctctatcg tgtataaagc tgacatcctg taattgtaat
     1381 tctgacgcta aatggtttaa aaattgaata cgcttattta atgaatcaac aatcgtcact
     1441 tttaactgcg gaaacattat ttttaacgga atacttggaa aaccagctcc agcgcctaca
     1501 tcacatatac ttataggctg attaaaatca aaataaaaac taggtgcaat ggaatcataa
     1561 aaatgtttca aatatacatc gtgttcatct gtaatacttg tcaaattcat cttttcattc
     1621 cattcaacaa gtaaacgata atatgtttga aactgttgtt tttgagtctc agttaattca
     1681 atattatgtt cttttaattg ttctgctaac cattctacag tcattagtca ctcacccttt
     1741 ggagtttacc ttgttctaag taaatcaata atatagatat gtctgctgga tttacccctg
     1801 atattctaga agcttgtgca atatttaaag gttttacttc tgataatttt tctcgcgctt
     1861 cagtcgccaa actatcaatc ttactataat ctaagtcttc tggaattttc ttctcttcca
     1921 tacgcttaac tttctcaact tgttgtagtg atttattgat ataaccttca tattttgttt
     1981 gtatttctac ttgttcttca acatctgcat tcaattgatg ttcttcttct aaaatttcta
     2041 aaattatatc gtaagtcatt tcaggtctgc gtaataaatc gatagctaaa ataccatctt
     2101 ttaagcgaga accaccatgt tgttcgataa tcgcttgcgt atgttcgttt ggtttaatac
     2161 gaatatctga taaacgctta atttccgcat caatttgctg acgtttttca ttaaaacgtg
     2221 catatcgttc ttcagaaatc ataccaagtt catatcccat atccgtcaat ctcaaatcag
     2281 cattatcatg acgtagtaac aaacgatatt ctgcacgtga tgttagtaaa cgataaggtt
     2341 cattagtacc tttagttaca agatcatcga ttaagacacc aatatatgca tctgaacgac
     2401 ttaatatctt ttcgcctgtg tttaacactt tacctgcagc gttaatacct gccatcaatc
     2461 cttgtcctgc tgcttcttca taaccagatg taccattaat ttgacctgca gtatataagt
     2521 ttttaatcat tttcgtttca agtgtaggcc ataactgcgt tggcacaatc gcatcatatt
     2581 caattgcgta gccggcacgc atcatatctg ctttttcaag acctggtatc gtctctaaca
     2641 tttgacgttg cacatgttca ggaagacttg tagacaatcc ttgcacatat acttcatttg
     2701 tattacgacc ttcaggctct aagaaaagtt gatgtcgcgg cttatcatta aatcgaacaa
     2761 atttatcttc aattgaaggg caataacgtg gcccggttcc tttaatcatc cctgaataca
     2821 ttgcagatag atgtaaatta tcatcgataa ctttgtgtgt ttcagcatta gtatacgtta
     2881 gccaacatgg caattgatct aatatatatt ctgttgtttc aaagctgaat gcacgaccta
     2941 catcgtcacc tggttgtatt tcagtcttcg aatagtcaat tgtttttgaa tttacacgcg
     3001 gtggtgtacc tgttttaaaa cgaacaatat caaaaccaag ttctcttaaa ttgtctgata
     3061 atgtgattga tggtaattgg tgatttggtc cacttgaata cttcatatta cctaaaatga
     3121 tttcaccacg taaaaatgtt cccgttgtaa taattactgc tttagataaa tactctgtac
     3181 caatatttgt acgtacacct ttaacttcat tatcttctat aataagttcg tctaccatac
     3241 cttgcattat atgcaaattt tcttcatctt caatcacgcg tttcatttct tgttgataaa
     3301 gtactttatc tgcttgcgct cttagtgctc ttacagcagg tcctttacct gtatttaaca
     3361 ttctcatttg aatgtgtgtt ttatcgattg tttttgccat ttgtccacct aaagcatcaa
     3421 tttcacgaac aacgatgcct ttagctggtc cacctacaga tgggttacat ggcataaatg
     3481 caatattatc taaatttatt gttagcatta atgttttagc accacgtctt gcagatgcta
     3541 aacctgcttc tacacctgca tgtcccgcac ctataacgat tacatcatat tcttgaacca
     3601 caatataaac ctccttattt gatatcttac tagccttctt aagacgatat tccgtctatt
     3661 tcaattacta tttacctaag cagaattgac tgaataactg atcgatgagt tcatcacttg
     3721 cagtctcacc aataatttct cctaatattt cccaagttct agttaaatca atttgtacca
     3781 tatccatagg cacaccagat tctgctgcat caatcgcatc ttgtatcgtt tgtcttgctt
     3841 gttttaataa tgaaatatgt cttgaattag aaacataagt catatcttga ttttgtactt
     3901 ctccaccaaa gaacaaatct cgaatttgta tttctaattc atcaatacct tcttgtttta
     3961 acattgaagt ttgaattaat ggcgtatcac ctatcatatc tttaacttca ttaatatcta
     4021 tgttttgctc taaatccatt ttattaacaa ttacgattac atcttcattt ttaaccactt
     4081 catataatgt gtaatcttct tgagtcaatg cttcgttatt gtttaataca aataaaatta
     4141 agtctgcttg gctaagagcc tttctagagc gttcaacacc aatcttctct actatatctt
     4201 ctgtctcgcg tataccagca gtatcaacta atcttaatgg cacgccacga acattgacgt
     4261 actcttctaa gacatctctt gtagtacctg ctacctcagt tacaatcgct ttattatctt
     4321 gtattaaatt atttaacatc gatgatttac ctacgtttgg tttaccaaca ataactgtag
     4381 ataaaccttc acgcataatt ttgccctgcg caccggtatc taataaacga ttaatttcct
     4441 gtttgatttc tttagactgc tctaaaagaa attcagtagt cgcatcttca acatcatcgt
     4501 attcaggata atcaatattc acttccactt gagcgagtat ctctaatata gattgacgtt
     4561 gttttttgat taagtcactt agacgacctt caatttgatt catcgcaact ttagaagctc
     4621 tatctgtctt cgagcgaata aagtccataa ctgcttcagc ttgagataaa tcaatacgac
     4681 catttaaaaa ggcacgtttt gtaaattcac ctggctcagc cattctagcg ccatatgtca
     4741 tagtaagttc cagcactcta ttaatcgtta aaataccacc atgacaatta atttctataa
     4801 tatcttcgcg tgtaaatgtt tttggcgctc ttaacacaga caccataact tcttcaacca
     4861 cttctttaga ctctggatca ataatatgac cgtaattaat cgtatgtgat ggaacatcat
     4921 ttaaaagatg ttttccttta tataatttgt cagcaatttc aacggcttgc ggtccagaca
     4981 atcgaacaat tccaattgcc ccttcaccca ttggtgttga aatactcgta attgtatcta
     5041 aatccatatt gctactcgcc tccttcaacg atgtgaatac attttaaagt aagttattat
     5101 aaccctaagg tcagtcttaa ccctaaggtc agtcttacgt ttgtctgagg taagacttcg
     5161 ggatgcgttg agtggttaat gttttccttc ccctacccta tccttactta atctttttat
     5221 taaaaacttt ggcaatttta agtacgtgct caagactatt ctgtatttgt aaagtcgtca
     5281 tatctttagc tggctgtctt gctattacaa taatatcttt ggccaatata tgcgacttat
     5341 gtactttgaa attttcacgt attgctcttt taatcttgtt tcttaacact gcattaccta
     5401 gttttttaga aacactaata cctaagcgaa aatggtctat ttctttatta ttacaagtgt
     5461 atacaacaaa ttgtctgttg gctacagaat gacctttttt atatattctc tgaaaatctg
     5521 cattcttttt aattcggtaa gctttttcca ataacatcac tcgcttattt atcgttttta
     5581 tttgaagcta tatttaaact tctattgagc ttataacata aatttctatt tattcttaat
     5641 ttaaacgaaa aaaaagatca ctgatgagtc agtgatctta tgcagataaa actttacggc
     5701 ctttacgacg acggcgcgct aaaactttac ggccattttt tgtgctcatg cgttttctga
     5761 aaccatgaac tttactatgt ttacgtttat ttggttgata agtacgtttt accatgcaaa
     5821 acacctccat ctttatacag ttatctaata taccagatta tgcagactct gatcacttta
     5881 atagataaga ttttgcgttt cttagattaa aattctcgta ttagctcatt gattatctag
     5941 tcataattca agcaactact acaatataac aaaatccttt ttataacgca agttcatttt
     6001 atactactgc tcaatttttt tacttttatc gattaaagat agaaatacac gatgcgagca
     6061 atcaaatttc ataacatcac catgagtttg gtccgaagca tgagtgttta caatgtttga
     6121 ataccttata cagttcttat acatacttta taaattattt cccaagctgt tttgatacac
     6181 tcactaacag atattctata gaaggaaaag ttatccactt atgcacattt atagttttca
     6241 gaattgtgga taattagaaa ttacacacaa agttatacta ttttcagcaa catattcaca
     6301 ggtatttgac atatagagaa ctgaaaaagt ataattgtgt ggataagtcg tccaactcat
     6361 gattttataa ggatttattt attgatattt acataaaaat actgtgcgta actaataagc
     6421 aggataaagt tatccaccga ttgttattaa cttgtggata attattaaca tggtgtgttt
     6481 agaagttatc cacggctgtt atttttgtgt ataacttaaa aatttaagaa agatggagta
     6541 aatttatgtc ggaaaaagaa atttgggaaa aagtgcttga aattgctcaa gaaaaattat
     6601 cagctgtaag ttactcaact ttcctaaaag atactgagct ttacacgatt aaagatggtg
     6661 aagctatcgt attatcgagt attcctttta atgcaaattg gttaaatcaa caatatgctg
     6721 aaattatcca agcaatctta tttgatgttg taggctatga agttaaacct cactttatta
     6781 ctactgaaga attagcaaat tatagtaata atgaaactgc tactccaaaa gaaacaacaa
     6841 aaccttctac tgaaacaact gaggataatc atgtgcttgg tagagagcaa ttcaatgccc
     6901 ataacacatt tgacactttt gtaatcggac ctggtaaccg ctttccacat gcagcaagtt
     6961 tagctgtagc cgaagcacca gccaaagcgt acaatccatt atttatctat ggaggggttg
     7021 gtttaggaaa aacccattta atgcatgcca ttggtcatca tgttttagat aataatccag
     7081 atgccaaagt gatttacaca tcaagtgaaa aattcacaaa tgaatttatt aaatcaattc
     7141 gtgataacga aggtgaagct ttcagagaaa gatatcgtaa tatcgacgtc ttattaatcg
     7201 atgatattca gttcatacaa aataaagtac aaacacaaga agaatttttc tatactttta
     7261 atgaattgca tcagaataac aagcaaatag ttatttcgag tgatcgacca ccaaaggaaa
     7321 ttgcacaatt agaagaccga ttacgttcac gctttgaatg ggggctaatt gttgatatta
     7381 cgccaccaga ttatgaaact cgaatggcaa ttttgcagaa gaaaattgaa gaagaaaaat
     7441 tagatattcc accagaagct ttaaattata tagcaaatca aattcaatct aatattcgtg
     7501 aattagaagg tgcattaaca cgtttacttg catattcaca attattagga aaaccaatta
     7561 caactgaatt aactgctgaa gctttaaaag atatcattca agcaccaaaa tctaaaaaga
     7621 ttaccatcca agatattcaa aaaattgtag gccagtacta taatgttagg attgaagatt
     7681 tcagtgcaaa aaaacgtaca aagtcaattg catatccgcg tcaaatagct atgtacttgt
     7741 ctagagagct tacagatttc tcattaccta aaattggtga agaatttggt gggcgtgatc
     7801 atacgaccgt cattcatgct catgaaaaaa tatctaaaga tttaaaagaa gatcctattt
     7861 ttaaacaaga agtagagaat cttgaaaaag aaataagaaa tgtataagta ggaaactttg
     7921 ggaaatgtaa tctgttatat aacagcacta ataataactg tcatttttta catttctata
     7981 tactaatgtg acaagatgag caaaactcat tttgtggata atgtttaaaa gtcatacaca
     8041 ccatacacaa gttatcaaca tgtgtataac ttcgccaaat ctatgttttt acgacttatc
     8101 caccaatcca cagcacctac tactattact aagaacttaa aacctatata attatatata
     8161 aacgactgga aggagtttta attaatgatg gaattcacta ttaaaagaga ttattttatt
     8221 acacaattaa atgacacatt aaaagctatt tcaccaagaa caacattacc tatattaact
     8281 ggtatcaaaa tcgatgcgaa agaacatgaa gttatactaa ctggttcaga ctctgaaatt
     8341 tcaatagaaa tcactattcc taaaactgta gatggcgaag atattgtcaa tatttcagaa
     8401 acaggctcag tagtacttcc tggacgattc tttgttgata ttataaaaaa attacctggt
     8461 aaagatgtta aattatctac aaatgaacaa ttccagacat taattacatc aggtcattct
     8521 gaatttaatt taagtggctt agatccagat caatatcctt tattacctca agtttctaga
     8581 gatgacgcaa ttcaattgtc ggtaaaagtg cttaaaaacg tgattgcaca aacgaatttt
     8641 gcagtgtcca cctcagaaac acgcccagta ctaactggtg tgaactggct tatacaagaa
     8701 aatgaattaa tatgcacagc gactgactca caccgcttgg ctgtaagaaa gttgcagtta
     8761 gaagatgttt ctgaaaacaa aaatgtcatc attccaggta aggctttagc tgaattaaat
     8821 aaaattatgt ctgacaatga agaagacatt gatatcttct ttgcttcaaa ccaagtttta
     8881 tttaaagttg gaaatgtgaa ctttatttct cgattattag aaggacatta tcctgataca
     8941 acacgtttat tccctgaaaa ctatgaaatt aaattaagta tagacaatgg ggagttttat
     9001 catgcgattg atcgtgcctc tttattagca cgtgaaggtg gtaataacgt tattaaatta
     9061 agtacaggtg atgacgttgt tgaattatct tctacatcac cagaaattgg tactgtaaaa
     9121 gaagaagttg atgcaaacga tgttgaaggt ggtagcctga aaatttcatt caactctaaa
     9181 tatatgatgg atgctttaaa agcaatcgat aatgatgagg ttgaagttga attcttcggt
     9241 acaatgaaac catttattct aaaaccaaaa ggtgacgact cggtaacgca attaatttta
     9301 ccaatcagaa cttactaaaa ataaatataa ataaaggatg acgtgattaa ttaaaacgtc
     9361 atcctttatt ttttggcaaa aataattcta gatgcgtatg taaaataaat ttgacagcat
     9421 tttaaacagc aaataaaaga cgccaattaa atttatgaca aatgtatcca aaatttaata
     9481 agtgtgctta tatgcccttt aaatttaaaa ttttaatagt caataacaag ttgaatatta
     9541 aagttaaacg ccgttaaata gcgttaaaaa attgaaaatg acagtattgc caaaaaataa
     9601 gaattaatta tttatatgta aacggtttct acctctattt taaatgaaat ttgtgacaaa
     9661 aaaaggtata atatattaat gacacacaaa gaaatggagt gattattttg gttcaagaag
     9721 ttgtagtaga aggagacatt aatttaggtc aatttctaaa aacagaaggg attattgaat
     9781 ctggtggtca agcaaaatgg ttcttgcaag acgttgaagt attaattaat ggagtgcgtg
     9841 aaacacgtcg cggtaaaaag ttagaacatc aagatcgtat agatatccca gaattacctg
     9901 aagatgctgg ttctttctta atcattcatc aaggtgaaca atgaagttaa atacactcca
     9961 attagaaaat tatcgtaact atgatgaggt tacgttgaaa tgtcatcctg acgtgaatat
    10021 cctcattgga gaaaatgcac aaggaaagac aaatttactt gaatcaattt ataccttagc
    10081 tttagcaaaa agtcatagaa cgagtaatga taaggaactc atacgtttta atgctgatta
    10141 tgctaaaata gaaggtgagc ttagttatag acacggcacg atgccattaa caatgtttat
    10201 aactaaaaaa ggtaaacaag tcaaagtgaa tcacttagag caaagtcgtc taactcaata
    10261 tattggacac ctcaatgtgg ttctatttgc gccagaagat ttgaatattg taaaaggctc
    10321 tcctcaaata agacgacgct ttatagatat ggagttgggc caaatttctg ctgtttactt
    10381 aaatgattta gctcaatacc aacgtatttt aaagcaaaag aataattact taaagcagtt
    10441 acaattaggc caaaaaaagg acttaacaat gttggaagta ttaaatcagc agtttgctga
    10501 atatgcaatg aaagtaactg ataaacgtgc acattttatt caagagctag agtcgttagc
    10561 taaaccgatt catgctggta tcacaaatga taaagaagcg ttgtcgctaa attatttacc
    10621 tagtcttaaa tttgattatg ctcaaaatga agcggcacga cttgaagaaa ttatgtctat
    10681 tcttagcgat aatatgcaaa gagaaaaaga acgaggcatt agcttattcg gaccacatcg
    10741 agatgatata agttttgatg tgaatggcat ggatgctcaa acatatggtt ctcaaggaca
    10801 gcaacgtaca acggctttgt ccattaaatt agctgaaatt gagttaatga atatcgaagt
    10861 tggggaatat cccatcttat tattagacga tgtactcagt gaattagatg attcgcgtca
    10921 aacgcattta ttaagtacga ttcagcataa agtacaaaca tttgtcacta cgacatctgt
    10981 agatggtatt gatcatgaaa tcatgaataa cgctaaattg tatcgtatta atcaaggtga
    11041 aattataaag taacagaaag cgatggtgac tgcattgtca gatgtaaaca acacggataa
    11101 ttatggtgct gggcaaatac aagtattaga aggtttagaa gcagtacgta aaagaccagg
    11161 tatgtatata ggatcgactt cagagagagg tttgcaccat ttagtgtggg aaattgtcga
    11221 taatagtatc gatgaagcat tagctggtta tgcaaataaa attgaagttg ttattgaaaa
    11281 agataactgg attaaagtaa cggataacgg acgtggtatc ccagttgata ttcaagaaaa
    11341 aatgggacgt ccagctgtcg aagttatttt aactgtttta catgctggtg gtaaattcgg
    11401 cggtggcgga tacaaagtat ctggtggttt acatggtgtt ggttcatcag ttgtaaacgc
    11461 attgtcacaa gacttagaag tatatgtaca cagaaatgag actatatatc atcaagcata
    11521 taaaaaaggt gtacctcaat ttgacttaaa agaagttggc acaactgata agacaggtac
    11581 tgtcattcgt tttaaagcag atggagaaat cttcacagag acaactgtat acaactatga
    11641 aacattacag cagcgtatta gagagcttgc tttcttaaac aaaggaattc aaatcacatt
    11701 aagagatgaa cgtgatgaag aaaacgttag agaagactcc tatcactatg agggcggtat
    11761 taaatcgtac gttgagttat tgaacgaaaa taaagaatct attcatgatg agccaattta
    11821 tattcatcaa tctaaagatg atattgaagt agaaattgcg attcaatata actcaggata
    11881 tgccacaaat cttttaactt acgcaaataa cattcatacg tatgaaggtg gtacgcatga
    11941 agacggattc aaacgtgcat taacgcgtgt cttaaatagt tatggtttaa gtagcaagat
    12001 tatgaaagaa gaaaaagata gactttctgg tgaagataca cgtgaaggta tgacagcaat
    12061 tatatctatc aaacatggtg atcctcaatt cgaaggtcaa acgaagacaa aattaggtaa
    12121 ttctgaagtg cgtcaagttg tagataaatt attctcagag cactttgaac gatttttata
    12181 tgaaaatcca caagtcgcac gtacagtggt tgaaaaaggt attatggcgg cacgtgcacg
    12241 tgttgctgcg aaaaaagcgc gtgaagtaac acgtcgtaaa tcagcgttag atgtagcaag
    12301 tcttccaggt aaattagccg attgctctag taaaagtcct gaagaatgtg agattttctt
    12361 agtcgaaggg gactctgccg gggggtctac aaaatctggt cgtgactcta gaacgcaggc
    12421 gattttacca ttacgaggta agatattaaa tgttgaaaaa gcacgattag atagaatttt
    12481 gaataacaat gaaattcgtc aaatgatcac agcatttggt acaggaatcg gtggcgactt
    12541 tgatctagcg aaagcaagat atcacaaaat cgtcattatg actgatgccg atgtggatgg
    12601 agcgcatatt agaacattgt tattaacatt cttctatcga tttatgagac cgttaattga
    12661 agcaggctat gtgtatattg cacagccacc gttgtataaa ctgacacaag gtaaacaaaa
    12721 gtattatgta tacaatgata gggaacttga taaacttaaa tctgaattga atccaacacc
    12781 aaaatggtct attgcacgat acaaaggtct tggagaaatg aatgcagatc aattatggga
    12841 aacaacaatg aaccctgagc accgcgctct tttacaagta aaacttgaag atgcgattga
    12901 agcggaccaa acatttgaaa tgttaatggg tgacgttgta gaaaaccgta gacaatttat
    12961 agaagataat gcagtttatg caaacttaga cttctaagcg ctgtgaactg aacttttgaa
    13021 ggaggaactc ttgatggctg aattacctca atcaagaata aatgaacgaa atattaccag
    13081 tgaaatgcgt gaatcatttt tagattatgc gatgagtgtt atcgttgctc gtgcattgcc
    13141 agatgttcgt gacggtttaa aaccagtaca tcgtcgtata ctatatggat taaatgaaca
    13201 aggtatgaca ccggataaat catataaaaa atcagcacgt atcgttggtg acgtaatggg
    13261 taaatatcac cctcatggtg actcatctat ttatgaagca atggtacgta tggctcaaga
    13321 tttcagttat cgttatccgc ttgttgatgg ccaaggtaac tttggttcaa tggatggaga
    13381 tggcgcagca gcaatgcgtt atactgaagc gcgtatgact aaaatcacac ttgaactgtt
    13441 acgtgatatt aataaagata caatagattt tatcgataac tatgatggta atgaaagaga
    13501 gccgtcagtc ttacctgctc gattccctaa cttgttagcc aatggagcat caggtatagc
    13561 ggtaggtatg gcaacgaata ttccaccaca taacttaaca gaattaatca atggtgtact
    13621 tagcttaagt aagaaccctg atatttcaat tgctgagtta atggaggata ttgaaggtcc
    13681 tgatttccca actgctggac ttattttagg taagagtggt attagacgtg catatgaaac
    13741 aggtcgtggt tcaattcaaa tgcgttctcg tgcagttatt gaagaacgtg gaggcggacg
    13801 tcaacgtatt gttgtcactg aaattccttt ccaagtgaat aaggctcgta tgattgaaaa
    13861 aattgcagag ctcgttcgtg acaagaaaat tgacggtatc actgatttac gtgatgaaac
    13921 aagtttacgt actggtgtgc gtgtcgttat tgatgtgcgt aaggatgcaa atgctagtgt
    13981 cattttaaat aacttataca aacaaacacc tcttcaaaca tcatttggtg tgaatatgat
    14041 tgcacttgta aatggtagac cgaagcttat taatttaaaa gaagcgttgg tacattattt
    14101 agagcatcaa aagacagttg ttagaagacg tacgcaatac aacttacgta aagctaaaga
    14161 tcgtgcccac attttagaag gattacgtat cgcacttgac catatcgatg aaattatttc
    14221 aacgattcgt gagtcagata cagataaagt tgcaatggaa agcttgcaac aacgcttcaa
    14281 actttctgaa aaacaagctc aagctatttt agacatgcgt ttaagacgtc taacaggttt
    14341 agagagagac aaaattgaag ctgaatataa tgagttatta aattatatta gtgaattaga
    14401 aacaatctta gctgatgaag aagtattact acaattagtt agagatgaat taacagaaat
    14461 tcgagatcgt ttcggtgatg atcgtcgtac tgaaatccaa ttaggtggat ttgaagattt
    14521 agaagatgaa gatctcattc cagaagaaca aattgtaatt acactaagcc ataataacta
    14581 cattaaacgt ttgccggtat ctacatatcg tgctcaaaac cgtggtggtc gtggtgttca
    14641 aggtatgaat acattggaag aagattttgt cagtcaattg gtaactttaa gtacacatga
    14701 ccatgtattg ttctttacta acaaaggtcg tgtatacaaa cttaaaggtt atgaagtgcc
    14761 tgagttatca agacagtcta aaggtattcc tgtagtgaat gctattgaac ttgaaaatga
    14821 tgaagtcatt agtacaatga ttgctgttaa agaccttgaa agtgaagaca acttcttagt
    14881 gtttgcaact aaacgtggtg tcgttaaacg ttcagcatta agtaacttct caagaataaa
    14941 tagaaatggt aagattgcga tttcgttcag agaagatgat gagttaattg cagttcgctt
    15001 aacaagtggt caagaagata tcttgattgg tacatcacat gcatcattaa ttcgattccc
    15061 tgaatcaaca ttacgtcctt taggccgtac agcaacgggt gtgaaaggta ttacacttcg
    15121 tgaaggtgac gaagttgtag ggcttgatgt agctcatgca aacagtgttg atgaagtatt
    15181 agtagttact gaaaatggtt atggtaaacg tacgccagtt aatgactatc gtttatcaaa
    15241 tcgtggtggt aaaggtatta aaacagctac gattactgag cgtaatggta atgttgtatg
    15301 tatcactaca gtaactggtg aagaagattt aatgattgtt actaatgcag gtgtcattat
    15361 tcgactagat gttgcagata tttctcaaaa tggtcgtgca gcacaaggtg ttcgcttaat
    15421 tcgcttaggt gatgatcaat ttgtttcaac ggttgctaaa gtaaaagaag atgcagaaga
    15481 tgaaacgaat gaagatgagc aatctacttc aactgtatct gaagatggta ctgaacaaca
    15541 acgtgaagcg gttgtaaatg atgaaacacc aggaaatgca attcatactg aagtgattga
    15601 ttcagaagaa aatgatgaag atggacgtat tgaagtaaga caagatttca tggatcgtgt
    15661 tgaagaagat atacaacaat catcagatga agatgaagaa taataaaaaa ataagacttc
    15721 cctatatgta ggggagtctt atttttatgc tagaaagtaa tgctgtacta tattcaatga
    15781 ttagtaatga ttaactttct aattgtttca ttgcgtaagg tatttcattg ataagtcttg
    15841 atggtggtac cacatacata tcttttgcaa ggttttcgcc aataaaacta tgtgtatatg
    15901 tggcactcat aaccgcttct tttaagttat caaattgacc gacaaaactt gtaatcatac
    15961 cagcaagtgt atcgcccata ccaccagtcg ccattgctgg gctaccgatt gttaatttaa
    16021 agtcttcatc tttaaagaaa atttcagtac catgtttttt aagtacaaca gttgcaccta
    16081 aacgatcaac tgcttcacga ttacgctcat atgtctgttc ctcaatagga ataccactta
    16141 atcgttccca ttctttgagg tgtggagtaa agatcacacg acatgtaggt aattgcggtt
    16201 tcagtttact aaagattgta atcgcatcgc cgtctacgat taaattttga tgcggttgta
    16261 tattttgtag taggaatgta atggcattat ttcctttgaa atcaacgcca agacctggac
    16321 caattagtat actgtcagtc atttcaatca ttttcgtcaa cattttcgta tcattaatat
    16381 caataaccat cgcttctggg caacgagaat gtaatgctga atgatttgtt ggatgtgtag
    16441 ctacagtgat taaaccacta ccgctaaata cacatgcacg agccgctaac ataatggcac
    16501 cacctaagtt agcagatcca ccaattaata aaattttgcc ataatcacct ttatgtgaat
    16561 cttctttacg cttaggaatg ttaatagaat ttaacgtttc catagtgata taacctccca
    16621 tgtaaaagcc ttttccaaat ttattcaatt ttaaaaatat atagtaactt ttaacaaaat
    16681 gtattataaa tttctgaatt cattattatt tgtcgttaaa tacaatagaa aatactatac
    16741 ctgtatatgc aattcgtcaa tagataaatt attaaatatg cttacaacaa tcttaatatc
    16801 ctttaacgca ctacaatagt gctctgataa taggttataa atgtacgtaa aaccattgtt
    16861 tcaataaaaa tgaaaacgta tacttcaaga aggatgggtt acttaatata aacaaggggg
    16921 taacatatat gactttatat ttagatggtg aaacactaac aattgaggat attaaatcat
    16981 ttttacaaca acaatcaaag attgaaatta ttgatgatgc gttagaacgt gtcaaaaaaa
    17041 gtagagcggt agttgaacgt attattgaaa atgaggaaac ggtttacggt atcactacag
    17101 gttttgggtt atttagtgat gtacgtatag acccgacgca atataatgaa ttacaagtga
    17161 atctgatacg ctcacatgcc tgtggactag gtgagccatt ttcaaaagaa gtagcattag
    17221 tcatgatgat tttacgattg aatacattat taaaaggtca ttcaggtgcc actttagaat
    17281 tagtgagaca attacaattt tttataaatg aacgtattat accgataatc ccacaacaag
    17341 gctctctcgg tgcatcagga gatttagcgc cattatcaca tttagcatta gcattaattg
    17401 gtgaagggaa agtattgtac agaggggaag aaaaggatag tgacgatgta ttaagagaat
    17461 taaatagaca acctttgaac cttcaggcta aagagggttt agcattgatt aatggtacgc
    17521 aagctatgac agctcaaggt gtcattagtt atatagaagc agaagattta ggttaccaat
    17581 ctgaatggat tgctgcatta acgcatcagt ctcttaatgg cattatagat gcatatcgac
    17641 atgatgtgca ctctgttcgt aattttcaag aacagattaa tgtggcagcg cgtatgcgtg
    17701 attggttaga aggatcaaca ttaacgacgc gacaagcaga aatacgtgta caagatgcat
    17761 atacgttgcg ttgtatacca caaatccatg gcgcgagttt tcaagtattc aattatgtta
    17821 aacagcaatt agaatttgaa atgaatgcgg ctaatgataa tccacttata tttgaggaag
    17881 caaatgaaac gtttgttatt tcaggtggta acttccatgg acaacctatt gcttttgcat
    17941 tagatcatct taaattaggt gtaagtgaat tagcaaacgt atcggaacgt cgtctagagc
    18001 gactagtaaa tcctcaatta aatggtgatt taccagcatt tcttagtcca gagccaggat
    18061 tgcaaagtgg cgcgatgatt atgcaatatg ctgctgcaag tctcgtttct gaaaataaaa
    18121 ctttagcgca tccagcgagt gttgattcta tcacttcatc tgcgaaccaa gaagatcacg
    18181 tatctatggg aactacagct gctagacatg gttatcaaat tattgaaaat gcaagacgtg
    18241 tgttggcaat cgaatgtgtt attgcattac aagcagcaga gttgaaaggt gtcgaaggat
    18301 tatcaccaaa aacacgtcgt aaatatgagg agtttcgaag tatcgtgcca tccattacac
    18361 atgaccgtca atttcataaa gatattgaag cggttgcaca gtatttaaag caatcaattt
    18421 atcaaacgac tgcatgtcac taaatcgaca tgagtgatgt ttgaaatcgt tacttgaaaa
    18481 agcaaatata gtttgctata ttaaaattaa cttaataaga cattgttcgt aggacaagta
    18541 atatatagtg ttcgatatca gagagcttgt ggttagtgtg aacaagaatc aacatatata
    18601 tgaatctacc tacttaattt aaaagaacaa tcggtgataa ccgttatttt agtgaagtgc
    18661 aatttaggtt tagtgtatct ttataactta aattgttaaa tagggtggca acgcgtagac
    18721 cacgtccctt gtagggatgt ggtctttttt tattttctaa aaaataaaga cgtacgtcat
    18781 tccataataa atgataatta tttgggaaag gatgaaggtt aatgttagac attagattat
    18841 tcagaaatga gcctgacaca gttaagagca aaattgaatt acgtggagat gatccaaaag
    18901 ttgtagatga aattttagaa ttggatgagc aacgacgtaa attaattagt gcaacagaag
    18961 aaatgaaagc acgtcgtaat aaagtaagcg aagaaatcgc attaaaaaaa cgtaataaag
    19021 aaaatgctga tgatgtgatt gctgaaatgc gcacattagg tgacgatatt aaagaaaaag
    19081 atagtcaatt aaatgaaatt gataataaaa tgacaggtat cctttgtcgt attccaaatt
    19141 taataagtga tgatgtacct caaggtgaat ctgatgaaga taacgttgaa gttaaaaagt
    19201 ggggtacacc acgtgagttt tcatttgaac caaaagcaca ttgggatatt gtagaagaat
    19261 tgaaaatggc tgattttgat cgtgcagcaa aagtttcagg tgcgcgtttt gtatatttaa
    19321 caaatgaagg tgcgcaatta gagcgtgctt taatgaacta tatgattaca aaacatacaa
    19381 cacaacatgg ttatacagaa atgatggtac cacagcttgt gaacgcagat acaatgtatg
    19441 gtacaggtca attacctaaa tttgaagaag atttatttaa agtagaaaaa gaaggattat
    19501 atacaattcc aactgctgaa gtaccattaa cgaatttcta ccgtaatgaa attattcaac
    19561 caggtgtact tcctgaaaaa ttcactggtc aatctgcatg tttccgtagt gaagcaggat
    19621 cagcaggtag agatacaaga ggattaattc gtttacatca attcgataaa gtggaaatgg
    19681 tacgttttga acaacctgaa gattcatgga atgctttaga agaaatgaca acaaatgcag
    19741 aagcaattct agaagagtta ggtttaccat accgtcgtgt tattttatgt acaggtgata
    19801 ttggatttag tgcaagcaaa acatatgata tagaagtttg gttaccaagc tacaatgatt
    19861 ataaagaaat tagttcatgc tcaaactgta cggatttcca agcgcgtcgt gctaacatcc
    19921 gcttcaagcg tgacaaagca gctaaaccag aattagcaca tacattaaat ggtagtggtt
    19981 tagcagttgg acgtacattt gctgctattg ttgaaaatta ccaaaatgaa gatggaacag
    20041 taacaattcc agaagcatta gtaccattta tgggtggtaa aacacaaatt tcaaaaccag
    20101 ttaaataaag gctttagcta caagctttaa aaagtatata ttctacgtat acttaaagca
    20161 agggcaagat actttaaata atattttaaa aagtggtgac gaagctgtcg ccactttttt
    20221 tgtgctgtaa aagtataata gtgagccaat gcacataaca acaataaatt aagtttgtgg
    20281 tttaatgggg tgaacgcatt tcattatagc aacaatacgg gataattatg atgaactaaa
    20341 acaatctaaa acgtaacaat tttgagcatc actattatag gaaattaagt gataaaaaac
    20401 tgatttcgtt gatgtagtat gagtaatatc gatggagtag ggtaggggga gaggatgatg
    20461 attataaggg agtgttacat gaatcaatat cccagactca tcattagata taaaaaatta
    20521 taaaattagt acttaaaaac aactacaaat ccgtagaaaa tatggaggta gtcttaaata
    20581 aaaattgaaa attatcaaaa ataaaaagtt gatatgaaga tgattgaata caccagtatg
    20641 tctaacctca gacaaactgg tgtctaatgt tattgcttag ggtatagaac tgtattagac
    20701 taggtatatt attttttcgt aattatataa atataaagtg gcaaaggagg taattgagat
    20761 ggcaacacat ttaagtttta gacaaggcgt acaagagtgt atcccaacat tattgggtta
    20821 tgccggtgtt ggtatttcat ttggtattgt ggcttcgtct caaaacttta gtattttaga
    20881 aattatcttg ttatgtcttg ttatatatgc cggtgctgcg caatttatta tgtgcgcgtt
    20941 gtttatagca ggtacaccga tatcagcgat tgtactaact gtatttatcg taaattcaag
    21001 aatgttcctt ttaagtatgt cgcttgcacc aaacttcaag acatatgggt tttggaaccg
    21061 tgttggatta ggttcattag taactgacga aacgtttggc gtcgccatta caccttattt
    21121 aaaaggagaa gctatcaatg atcgttggat gcatggtctt aatatcacag catatttatt
    21181 ttgggcaatt tcatgtgtag ctggggcttt atttggcgaa tatatctcaa atccgcaaac
    21241 gctagggtta gattttgcta tcacggctat gtttatcttt ttggccattg cgcaatttga
    21301 atcaattact aaatcgcgat taagaattta catagtactc attattgccg tcatagtaat
    21361 gatgttatcg ctaagtatgt ttatgccttc atatctagca atattaattg cagccacaat
    21421 ttcagcagcg ttaggagtga tgatggaacg atgataactc atatgaacat gttaatactt
    21481 attttattgt gtggtatcgt aacgctatta attcgaatta taccttttat catgatttca
    21541 aaagtgcaat tgcctgatgt tgtggttcga tggctatcat ttatcccaat cacactattt
    21601 acggcacttg tcattgacag cattattcaa cagacgcctc atggtgaggg gtatacatta
    21661 aacatccctt acattatcgc gctcattccg acggttattt tatctataat cacgcgtagt
    21721 ttaactatta caattattag tgggattgtt atcatggcaa cattacgatt tttcttttaa
    21781 aaataactga aaatcattga acagatattt aaacttaggt aaactgttag taatcagaaa
    21841 attctgatat acaaatcgtc tatggtatga ctgggaatcg tatacacgaa agcgatttgg
    21901 ctaacgtatg acaatgataa caatatttta aatgataaaa gtaattcatc actgaatctc
    21961 aactaacaca taacaatttc atatttctta ttgtgagaag ttgagggact tggccctgtg
    22021 atacttcagc aaccgacttt atagcacggt gctaaaacca acgagttact cgaatgataa
    22081 gtataaagac ttcttacttt tcaatagggt gagaagtttt tttgtttaag gaggaaagaa
    22141 caatgacaaa ttacacagta gatactttaa atctagggaa atttattaca gaatctgggg
    22201 aagtcataga taacttgcgt ttgagatatg agcatgttgg ttatcatgga caaccattag
    22261 ttgtagtttg tcatgcatta actggcaatc atttaacata tggaacagat gattatccgg
    22321 gttggtggcg agaaattatt gatgggggat atatacccat tcacgattat caatttttaa
    22381 catttgatgt tattggtagt cctttcggtt caagttcacc tttaaacgac cctcattttc
    22441 ctaaaaaatt aacattaaga gatatagtca gagcgaatga acgaggtata caagcccttg
    22501 gttatgataa gattaatatt ttaatagggg gaagtcttgg aggtatgcaa gcaatggaac
    22561 tactttacaa tcaacagttt gaagtagata aagccattat ccttgctgca acaagtcgaa
    22621 catcatctta tagtagagct ttcaatgaaa ttgcaaggca agccattcat cttggtggta
    22681 aggaaggtct aagtattgca cgccaattag gttttttgac atatcgatca tcaaaaagtt
    22741 atgatgaacg tttcacgccg gatgaagtag tcgcatacca acaacatcaa ggtaataaat
    22801 ttaaagaaca ttttgatttg aattgttatc tgacactgct agatgtattg gatagtcaca
    22861 acattgaccg aggtcgcaca gacgtaacgc atatttttaa aaatttagaa acgaaagtct
    22921 taacgatggg gttcatagat gatttgctat atccggacga tcaagttcgt gcattaggtg
    22981 aacgttttaa atatcatcgt catttcttcg tgcctgataa tgttggacat gatggattcc
    23041 tactaaactt tagtacctgg gcacctaact tatatcattt cttaaattta aagcatttta
    23101 agcgtaagta ataatgtata tatttttaaa gaatgaagct actaaatcaa agttatcata
    23161 ttcaaagaca tctagagtac atgagagtgg aaatgaattg taaatctaaa tcaagcttga
    23221 ttgctatgta gtgggattaa ataatcatgt tgaaaatgaa agcacacaaa agcgattcaa
    23281 atgagtatgt taattactta taaaacatga aagttattat tcaattaaat gaaatagaag
    23341 catacaatct ataaattgtt aatttccatt aaagaggtta aaataatagc tatagttaaa
    23401 aatatgggag tggcttatgt gttttcaaaa atacaaccta aagcaacaat aattgcaacg
    23461 attacgttgg tatttgtcgc tttagcttta tatctagtgc ctggtttggg actaatattt
    23521 gcattatttg caaccatacc aggtatcgtt ttatggaata aatcaataca atctttcggg
    23581 gttagtgcac ttattacagt aattataaca actgttttag gtaatacttt cgttttaagt
    23641 gccatcatat tagtcttaat tgcaagttta attattggtc aattgctcaa agaaagaacg
    23701 tctaaagaaa gaatgttata cgtaacaaca gtagcgatga gcttaatttc attaatcgct
    23761 tttatgttac tacaaacatt cggaaggatt ccaccatcag cgagcatagt aaaacctttc
    23821 aagcaaacat tacatgaagc gattacgatg agcggtgccg atgcgaatat gacccaaata
    23881 ttagaagaag ggtttagaca agcgaccgtt caattaccag gtttcatcat tatcattaca
    23941 tttttaatcg tcttaattaa cttaatcgtt acatttccgg ttttacgaaa atttaaaatc
    24001 gctacacctg tatttaagcc acttttcgcg tggcaaatga gcggtatttt attatggata
    24061 tacattattg ttatcatatg tttattattt acaggtcaac cgagtgtgtt ccagagcatt
    24121 cttttaaact tccaacttgt gttatcatta gtaatgtata ttcaaggttt aagtgttatt
    24181 catttctttg gtaaagcgaa aggtttgccg aatgcagtaa cgattttact attgattatc
    24241 ggtacaatac tgacacctac gacacatatt gtaggactac ttggtgttat cgatttaagt
    24301 ttgaatttga agcgaatcat gaaaaataat tctaaaaagt gaatagaggt ggaataatga
    24361 atcggcagtc cactaagaaa gctttactaa taccatttgt catcatgatc atcacagcaa
    24421 ttgttttaat gggtgtatgg tttatcttta atagtcttat agcattaatt gcatctatcg
    24481 ttcttgtcgt gatgattatt gttagcatca ttttattcag acaagcttta atgaaaatgg
    24541 atagttatgt agatggtttg agtgctcaaa tttcaacaac aaataataaa gcaatcaaac
    24601 atttaccaat tggtatcatt gttttagatg aaaatgatca catcgaatgg gttaaccaat
    24661 ttatgacaaa tcatatggaa gcaaatgtca tttctgaatc tgtaaatgaa gtatttccaa
    24721 acattttaaa gcaattagat agagtgaaat ccgttgaaat agaatataat cagtatcatt
    24781 tccaagtacg ttattctgag aatgatcatt gcctctattt ctttgatata actgaacaag
    24841 tacaaacaaa tgaactatat gaaaattcta aaccaatcat tgcgacatta tttttagata
    24901 actacgatga gattactcaa aatatgaatg atacgcagcg ttcggaaatc aattcaatgg
    24961 taacgcgtgt tattagtcga tgggcaactg agtataatat attctttaaa aggtatagtt
    25021 ccgatcaatt cgtagcctat ttaaatcaaa aaatattagc tgacttagaa gaatctaaat
    25081 ttgatatctt gagtcaatta cgtgaaaaaa gtgttggtta tcgtgcccaa ttaacattaa
    25141 gtattggtgt tggtgaaggt actgaaaatt taatcgattt aggtgaatta tcacaatcag
    25201 gcctagactt agcattaggg cgcggtggcg accaagttgc aattaaaagt attaatggta
    25261 atgtgcgttt ctatggcggt aagactgacc cgatggagaa acgtactcgt gtaagagcac
    25321 gagtgatctc acatgcgtta aaagatatcc ttgcagaggg tgacaaagtc attatcatgg
    25381 gacataaacg tcctgactta gatgcaattg gtgcagcaat cggtgtgtct agatttgcaa
    25441 tgatgaataa tttagaagca tacatcgtat taaatgagac tgacattgat ccaacattac
    25501 gacgcgtgat gaacgaaatt gataaaaagc cagagttaag agagcgattt ataacatcag
    25561 atgatgcttg ggatatgatg acatctaaga caaccgtagt gattgttgat acgcataaac
    25621 cggaactggt tttagatgaa aatgtcttaa ataaagcaaa ccgtaaagtt gttatcgatc
    25681 atcatagacg tggtgaaagc ttcatctcta atccattgtt gatatatatg gaaccatacg
    25741 caagttcaac agctgaattg gtaacagagt tactggaata tcaaccaaca gaacaacgtt
    25801 taacacgtct tgaatcaaca gtgatgtatg caggtattat tgtagataca agaaacttta
    25861 cattacgaac aggatcaaga acattcgatg cagcgagtta tttacgtgca catggtgcag
    25921 atacgatttt aacgcaacat ttcttaaaag atgatgtgga tacttacatt aatcgatctg
    25981 aattaattcg aactgtaaaa gttgaagata atggcatagc cattgcgcat ggttcagacg
    26041 ataaaattta tcatccagta acagttgcac aagcagcaga tgaactgtta agtttagaag
    26101 gtattgaagc atcatatgtt gttgcgagac gtgaagataa tctgattggt atatctgcgc
    26161 gttcactcgg ttcagtaaat gttcagttaa caatggaagc acttggtggc ggtggacatt
    26221 taaccaatgc tgcaacacaa cttaaaggtg tgacagtcga agaggcgata gcacaattac
    26281 aacaagcaat tacagaacaa ttaagtagga gtgaagatgc atgaaagtaa tttttacaca
    26341 agatgttaaa ggtaaaggta aaaaaggtga agttaaagaa gtaccagtag gttatgcaaa
    26401 taacttctta ttgaaaaaga attatgctgt agaagcaaca ccaggtaacc ttaaacaatt
    26461 agagttacag aaaaaacgtg caaaacaaga acgccaacaa gaaattgaag atgctaaagc
    26521 attaaaagaa acgttatcaa acattgaagt tgaagtatca gcaaaaactg gtgaaggtgg
    26581 taaattgttt ggatcagtaa gtacaaaaca aattgccgaa gcactaaaag cacaacatga
    26641 tattaaaatt gataaacgta aaatggattt accaaatgga attcattccc taggatatac
    26701 gaatgtacct gttaaattag ataaagaagt tgaaggtaca attcgcgtac acacagttga
    26761 acaataaagt tggattgaaa taagaggtgt aaccattcat ggatagaatg tatgagcaaa
    26821 atcaaatgcc acataacaat gaagctgaac agtctgtctt aggttcaatt attatagatc
    26881 cagaattgat taatactact caggaagttt tgcttcctga gtcgttttat aggggtgccc
    26941 atcaacatat tttccgtgca atgatgcact taaatgaaga taataaagaa attgatgttg
    27001 taacattgat ggatcaatta tcgacggaag gtacgttgaa tgaagcgggt ggcccgcaat
    27061 atcttgcaga gttatctaca aatgtaccaa cgacgcgaaa tgttcagtat tatactgata
    27121 tcgtttctaa gcatgcatta aaacgtagat tgattcaaac tgcagatagt attgccaatg
    27181 atggatataa tgatgaactt gaactagatg cgattttaag tgatgcagaa cgtcgaattt
    27241 tagagctatc atcttctcgt gaaagcgatg gctttaaaga cattcgagac gtcttaggac
    27301 aagtgtatga aacagctgaa gagcttgatc aaaatagtgg tcaaacacca ggtataccta
    27361 caggatatcg agatttagac caaatgacag cagggttcaa ccgaaatgat ttaattatcc
    27421 ttgcagcgcg tccatctgta ggtaagactg cgttcgcact taatattgca caaaaagttg
    27481 caacgcatga agatatgtat acagttggta ttttctcact agagatgggt gctgatcagt
    27541 tagccacacg tatgatttgt agttctggta atgttgactc aaaccgctta agaacgggta
    27601 ctatgactga ggaagattgg agtcgtttta ctatagcggt aggtaaatta tcacgtacga
    27661 agatttttat tgatgataca ccgggtattc gaattaatga tttacgttct aaatgtcgtc
    27721 gattaaagca agaacatggc ttagacatga ttgtgattga ctacttacag ttgattcaag
    27781 gtagtggttc acgtgcgtcc gataacagac aacaggaagt ttctgaaatc tctcgtacat
    27841 taaaagcatt agcccgtgaa ttagaatgtc cagttatcgc attaagtcag ttatctcgtg
    27901 gtgttgaaca acgacaagat aaacgtccaa tgatgagtga tattcgtgaa tctggttcga
    27961 ttgagcaaga tgccgatatc gttgcattct tataccgtga tgattactat aaccgtggcg
    28021 gcgatgaaga tgatgacgat gatggtggtt tcgagccaca aacgaatgat gaaaacggtg
    28081 aaattgaaat tatcattgct aagcaacgta acggtccaac aggcacagtt aagttacact
    28141 ttatgaaaca atataataaa tttaccgata tcgattatgc acatgcagat atgatgtaaa
    28201 aaagtttttc cgtacaataa tcattaagat gataaaattg tacggttttt attttgttct
    28261 gaacgggttg atatatgtta agtttgtgta ttgaaagtga taaattagta ctgtcaacgc
    28321 ctctgttaaa gggtttttag gacgttgaag acgatttgtt aaaatgattt ttcttttaaa
    28381 aaggccgaaa atcaatgttc gatttttatt tgcattatgg tctcgatatt ggtagaatat
    28441 caaatggtta aatgagaaaa acttggaggt gctcacatgt catcaatcgt agtagttggg
    28501 acacaatggg gagacgaagg aaaaggaaaa ataacggatt tcttggcaga acagtcagat
    28561 gttatcgcgc gtttttcagg tggtaataat gcaggccata ccattcaatt tggcggagaa
    28621 acatataaat tacatttagt accatctggt atcttttaca aagacaaatt agcggtaatc
    28681 ggtaacggtg tcgttgttga tccagttgca ctattgaaag aattagacgg attaaatgaa
    28741 cgtggcattc ctacaagtaa tttacgtata tctaatcgtg cgcaagtgat tttaccatat
    28801 cacttagcac aagatgaata tgaagaacgt ttacgcggtg acaataagat tggtacaact
    28861 aaaaaaggta tcggtccagc atatgtagac aaagttcaac gtatcggtat tcgtatggca
    28921 gatttacttg aaaaagaaac attcgaaaga ttattaaaat caaacattga atataaacaa
    28981 gcatatttca aaggtatgtt taacgaaaca tgtccatcat ttgatgatat ctttgaagaa
    29041 tactatgctg caggtcaacg tttaaaagaa tttgtaacag acacatcaaa aatcttagac
    29101 gatgcatttg tagcagatga aaaggtactt ttcgaaggtg cgcaaggtgt aatgttagat
    29161 atcgaccatg gtacatatcc attcgttaca tcaagtaatc caattgcagg taacgttact
    29221 gttggtacag gtgtaggtcc tacattcgtt tcgaaggtaa ttggtgtatg taaagcttat
    29281 acatcacgtg ttggtgatgg tccattccct actgaattat tcgatgaaga tggacatcat
    29341 attagagaag ttggtcgtga atacggtaca acaacaggac gtccacgtcg tgtaggttgg
    29401 tttgactcag ttgtattacg tcactctcgt cgtgtaagtg gtattacaga tttatctatt
    29461 aactcaatcg atgttttaac aggcctagac acagtgaaaa tctgtacagc ttatgaatta
    29521 gacggtaaag aaattactga gtacccagca aacttagatc aattaaaacg ttgtaaacca
    29581 atctttgaag agttaccagg ttggacagaa gacgtaacaa gtgtgcgtac tttagaagaa
    29641 ttacctgaaa atgcacgtaa atatttagag cgtatttcag aattatgtaa tgtacaaatt
    29701 tctatcttct cagttggtcc agatagagaa caaacaaacc tattaaaaga attgtggtag
    29761 aactttatat aagtcatacg caatgattat aaatacatga gccttctatc tttattggta
    29821 ggaggctttt gctttgtttg cttctgtatc gattcgatta tttagataaa aattactaac
    29881 gtaaaggtga tatttgctag tcataattta aaagattaga tgatatttaa caaaattaag
    29941 aagaaatact tgaatttaat aagtctgatg tcgaaaatag ctattaaaat agagtagacg
    30001 taagtgtaaa tgaaagtacc taaaatagaa aaatttcaaa aatagcgtaa ttattataat
    30061 aaatagactg ccaataaaat gcaatttttc acttataaca ttcttcaaaa aataatagca
    30121 aaattatgta aaaaatatct tgtcatggca agattggctg tgctataatc tatcttgtgc
    30181 ttaagaacgg ctccttggtc aagcggttaa gacaccgccc tttcacggcg gtaacacggg
    30241 ttcgagtccc gtaggagtca ccatttttta ggtctcgtag tgtagcggtt aacacgcctg
    30301 cctgtcacgc aggagatcgc gggttcgatt cccgtcgaga ccgtacaaat gcctatccaa
    30361 gaggataggc atttttttgc gtttaatatt atattaataa aagatatatg gacgaatgat
    30421 aatcatattg atttatctgt tcgtccattt tctttataat gtatgaacct caagtaactt
    30481 agtggttgga tatgaaagat aaacgtagac aataaaatct ttattagacg tacaaacata
    30541 tgctactgtc aacatatttc ttcgttgtga tatgccacca atcctccata acatcaattg
    30601 ttaaagtaac gaataacgaa taatgatatt tattttctga gcaatgacgt gcaactagaa
    30661 gttgccatta ccctaatttt attattggaa tagagacctc atcattgtgt taaatatcat
//
LOCUS       DAOBBH010000012        28743 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.012, whole genome shotgun sequence.
ACCESSION   DAOBBH010000012 DAOBBH010000000
VERSION     DAOBBH010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 28743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28743
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.012"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..528)
                     /locus_tag="P6L01_000360"
     CDS             complement(<1..528)
                     /locus_tag="P6L01_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HDP3029457.1"
                     /translation="MKLKNIAKASLALGILTTGMITTTAQPVKASTLEVRSQATQDLS
                     EYYKGRGFELTNVTGYKYGNKVTFIDNSQQIDVTLTGNEKLTVKDDDEVSNVDVFVVR
                     EGSDKSAITTSIGGITKTNGTQHKDTVQNVNLSVSKSTGQHTTSVTSEYYSIYKEEIS
                     LKELDFKLRKHLIDKH"
     gene            complement(911..2122)
                     /locus_tag="P6L01_000361"
     CDS             complement(911..2122)
                     /locus_tag="P6L01_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDP3029458.1"
                     /translation="MSNTQKKNVPELRFPGFEGEWEEKQLGDLTDRVIRKNKNLESKK
                     PLTISGQLGLIDQTEYFSKSVSSKNLENYTLIKNGEFAYNKSYSNGYPLGAIKRLTRY
                     DSGVLSSLYICFSIKSEMSKDFMEAYFDSTHWYREVSGIAVEGARNHGLLNVSVNDFF
                     TILIKYPSLEEQQKIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDEN
                     GEDYPDWENSKIEKYLKERNERSDKGQMLSVTINSGIIKFSELDRKDNSSKDKSNYKV
                     VRKNDIAYNSMRMWQGASGKSNYNGIVSPAYTVLYPTQNTSSLFIGYKFKTHRMIHKF
                     KINSQGLTSDTWNLKYKQLKNINIDIPVLEEQEKIGDFFKKMDILISKQKMKIEILEK
                     EKQSFLQKMFL"
     gene            complement(2115..3671)
                     /locus_tag="P6L01_000362"
     CDS             complement(2115..3671)
                     /locus_tag="P6L01_000362"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDP3029459.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVT
                     DGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYEN
                     FDIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADF
                     AFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYG
                     TSIPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKETIDK
                     YSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAY
                     LKELGVLKDE"
     gene            3749..3850
                     /locus_tag="P6L01_000363"
     CDS             3749..3850
                     /locus_tag="P6L01_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029460.1"
                     /translation="MENFDVGGGIFIFMEIGIGFMTHKQKRLNDDID"
     gene            complement(3935..4618)
                     /locus_tag="P6L01_000364"
     CDS             complement(3935..4618)
                     /locus_tag="P6L01_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL10"
                     /protein_id="HDP3029461.1"
                     /translation="MKFTALAKATLALGILTTGTLTTEVHSGHAKQNQKSVNKHDKEA
                     LYRYYTGKTMEMKNISALKHGKNNLRFKFRGIKIQVLLPGNDKSKFQQRSYEGLDVFF
                     VQEKRDKHDIFYTVGGVIQNNKTSGVVSAPILNISKEKGEDAFVKGYPYYIKKEKITL
                     KELDYKLRKHLIEKYGLYKTISKDGRVKISLKDGSFYNLDLRSKLKFKYMGEVIESKQ
                     IKDIEVNLK"
     gene            complement(4984..5682)
                     /locus_tag="P6L01_000365"
     CDS             complement(4984..5682)
                     /locus_tag="P6L01_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL9"
                     /protein_id="HDP3029462.1"
                     /translation="MKLTALAKATLALGILTTGVFTAESKAVHAKVELDETQRKYYIN
                     MLHQYYSEESFESTNISVKSEDYYGSNVLNFNQRNKTFKVFLLGDDKNKYKEKTHGLD
                     VFAVPELIDIKGGIYSVGGITKKNVRSVFGFVSNPSLQVKKVDAKHGFSINELFFIQK
                     EEVSLKELDFKIRKMLVEKYRLYKGASDKGRIVINMKDEKKYVIDLSEKLSFDRMFDV
                     MDSKQIKNIEVNLN"
     gene            complement(6059..6757)
                     /locus_tag="P6L01_000366"
     CDS             complement(6059..6757)
                     /locus_tag="P6L01_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL8"
                     /protein_id="HDP3029463.1"
                     /translation="MKFTAIAKAIFVLGILTTSVMITENQSVNAKGKYEKMNRLYDTN
                     KLHQYYSGPSYELTNVSGRSQGYYDSNVLLFNQQNQKFQVFLLGKDENKYKEKTHGLD
                     VFAVPELVDLDGRIFSVSGVTKKNVKSIFESLRTPNLLVKKIDDKDGFSIDEFFFIQK
                     EEVSLKELDFKIRKLLIKKYKLYEGSADKGRIVINMKDENKYEIDLSDKLDFERMADV
                     INSEQIKNIEVNLK"
     gene            complement(7096..7791)
                     /locus_tag="P6L01_000367"
     CDS             complement(7096..7791)
                     /locus_tag="P6L01_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL7"
                     /protein_id="HDP3029464.1"
                     /translation="MKLKTLAKATLALGLLTTGVITSEGQAVHAKEKQERVQHLYDIK
                     DLYRYYSSESFEFSNISGKVENYNGSNVVRFNQEKQNHQLFLLGKDKDKYKKGLEGQN
                     VFVVKELIDPNGRLSTVGGVTKKNNKSSETNTHLFVNKVYGGNLDASIDSFLINKEEV
                     SLKELDFKIRKQLVEKYGLYKGTTKYGKITINLKDEKKEVIDLGDKLQFERMGDVLNS
                     KDIQNIAVTINQI"
     gene            complement(8237..8941)
                     /locus_tag="P6L01_000368"
     CDS             complement(8237..8941)
                     /locus_tag="P6L01_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL5"
                     /protein_id="HDP3029465.1"
                     /translation="MKMAAIAKASLALGILATGTITSLHQTVNASEHEAKYENVTKDI
                     FDLRDYYSGASKELKNVTGYRYSKGGKHYLIFDKNRKFTRVQIFGKDIERFKARKNPG
                     LDIFVVKEAENRNGTVFSYGGVTKKNQDAYYDYINAPRFQIKRDEGDGIATYGRVHYI
                     YKEEISLKELDFKLRQYLIQNFDLYKKFPKDSKIKVIMKDGGYYTFELNKKLQTNRMS
                     DVIDGRNIEKIEANIR"
     gene            complement(9305..10182)
                     /locus_tag="P6L01_000369"
                     /pseudo
     CDS             complement(9305..10182)
                     /locus_tag="P6L01_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498975.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL4"
     gene            complement(10291..10428)
                     /locus_tag="P6L01_000370"
     CDS             complement(10291..10428)
                     /locus_tag="P6L01_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029466.1"
                     /translation="MFVKVAFLCLKSDESSNVPSVESHQNQFYLTNIMDFLIYLTMIQ
                     I"
     gene            complement(10546..11616)
                     /locus_tag="P6L01_000371"
     CDS             complement(10546..11616)
                     /locus_tag="P6L01_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL3"
                     /protein_id="HDP3029467.1"
                     /translation="MNMKTIAKTSLALGLLTTGAITVTTQSVKAEKIQSTKVDKVPTL
                     KAERLAMINITAGANSATTQAANTRQERTPKLEKAPNTNEEKTSASKIEKISQPKQEE
                     QKSLNISATPAPKQEQSQTTTESTTPKTKVTTPPSTNTPQPMQSTKSDTPQSPTIKQA
                     QTDMTPKYEDLRAYYTKPSFEFEKQFGFLLKPWTTVRFMNVIPNRFIYKIALVGKDEK
                     KYKDGPYDNIDVFIVLEDNKYQLKKYSVGGITKTNSKKVDHKAELSVTKKDNQGMISR
                     DVSEYMITKEEISLKELDFKLRKQLIEKHNLYGNMGSGTIVIKMKNGGKYTFELHKKL
                     QEHRMADVIEGTNIDKIEVNIK"
     gene            complement(11907..12602)
                     /locus_tag="P6L01_000372"
     CDS             complement(11907..12602)
                     /locus_tag="P6L01_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL2"
                     /protein_id="HDP3029468.1"
                     /translation="MKMKNIAKISLLLGILATGVNTTTEKPVHAEKKPIVISENSKKL
                     KAYYTQPSIEYKNVTGYISFIQPSIKFMNIIDGNSVNNLALIGKDKQHYHTGVHRNLN
                     IFYVNEDKRFEGAKYSIGGITSANDKAVDLIAEARVIKADHIGEYDYDFFPFKIDKEA
                     MSLKEIDFKLRKYLIDNYGLYGEMSTGKITVKKKYYGKYTFELDKKLQEDRMSDVINV
                     TDIDRIEIKVRKA"
     gene            complement(12888..13568)
                     /locus_tag="P6L01_000373"
     CDS             complement(12888..13568)
                     /locus_tag="P6L01_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL1"
                     /protein_id="HDP3029469.1"
                     /translation="MKFKAIAKASLALGMLATGVITSNVQSVQAKTEVKQQSESELKH
                     YYNKPVLERKNVTGYKYTEKGKDYIDVIVDNQYSQISLVGSDKDKFKDGDNSNIDVFI
                     LREGDSRQATNYSIGGVTKTNSQPFIDYIHTPILEIKKGKEEPQSSLYQIYKEDISLK
                     ELDYRLRERAIKQHGLYSNGLKQGQITITMKDGKSHTIDLSQKLEKERMGDSIDGRQI
                     QKILVEMK"
     gene            14040..14885
                     /locus_tag="P6L01_000374"
     CDS             14040..14885
                     /locus_tag="P6L01_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDP3029470.1"
                     /translation="MNIMLTGATGHLGTHITNQAIANHIDHFHIGVRNVEKVPDDWRG
                     KVSVRQLDYFNQESMVEAFKGMDTVVFIPSIIHPSFKRIPEVENLVYAAKQSGVAHII
                     FIGYYADQHNNPFHMSPYFGYASRLLSTSGIDYTYVRMAMYMDPLKPYLPELMNMHKL
                     IYPAGDGRINYITRNDIARGVIAIIKNPDTWGKRYLLSGYSYDMKELAAILSEASGTE
                     IKYEPVSLETFAEMYDEPKGFGALLASMYHAGARGLLDQESNDFKQLVNDQPQTLQSF
                     LQENI"
     gene            14904..15263
                     /locus_tag="P6L01_000375"
     CDS             14904..15263
                     /locus_tag="P6L01_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HDP3029471.1"
                     /translation="MNIISTILIIFMALEFFYIMYLETIATTSKKTSETFNISVDKLK
                     DKNINLLLKNQGVYNGLIGVLLIYGLFISSNPKEICTAILVYIIGVAIYGGLSSNISI
                     FFKQGTLPVLALISMLW"
     gene            15795..15947
                     /locus_tag="P6L01_000376"
     CDS             15795..15947
                     /locus_tag="P6L01_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029472.1"
                     /translation="MVPEEKGSITLSKEAAIIFATAKFKPFKNRIKNNPLKTNPFLKL
                     HENKKS"
     gene            16208..16480
                     /locus_tag="P6L01_000377"
     CDS             16208..16480
                     /locus_tag="P6L01_000377"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDP3029473.1"
                     /translation="MTKERRTFSSEFKLQMVRLYKNGKLKNEIIRKYDLKPSIISNSI
                     KQHQNTGPFNHQDNLKSDEKELIKLRKEVQHLKMEHDVLKQILKLI"
     gene            16879..17583
                     /locus_tag="P6L01_000378"
     CDS             16879..17583
                     /locus_tag="P6L01_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDP3029474.1"
                     /translation="MSKNINQANSKLNTSNKKLKQAYSKSDSKNMKVIYMPHWLEFYS
                     IAIHSDVTSNKKRYYKSFSRGTVVYVKLGSNIGSEFSGNHFCVILDNKDNKGKETVTI
                     VPLSSKGNKNYLKLNESVLNLTATDLKKQIIDISSKVQALTQKYRDIDIKLSTEDKKL
                     LSNLNQKANELYRVIGVYSKHKGKDTYVNISAITTISKRRISKINDSDPTGTIIISED
                     DMNEIEKQLKIKFFSN"
     gene            17975..18511
                     /locus_tag="P6L01_000379"
     CDS             17975..18511
                     /locus_tag="P6L01_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029475.1"
                     /translation="MHNKNEFNKKLKDFIGSDKRMALVKGYINEYKLTTVLRALNNSD
                     YTKGTIHTRSIGNLKQIVDNRIIPKNITQNKIFKLSNLTLEVNLYERNTIYHGDFAIY
                     YPVESALIIETDTPRLINHINNNPVQKIFIITTNDWSFNTLELEKIVDETIIYDLKQE
                     DPKQYEILYKNKHGKLPY"
     gene            complement(18624..20165)
                     /gene="guaA"
                     /locus_tag="P6L01_000380"
     CDS             complement(18624..20165)
                     /gene="guaA"
                     /locus_tag="P6L01_000380"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDP3029476.1"
                     /translation="MEMAKEQELILVLDFGSQYNQLITRRIREMGVYSELHDHEISIE
                     EIKKMNPKGIILSGGPNSVYEEGSFTIDPEIYNLGIPVLGICYGMQLTTKLLGGKVER
                     ANEREYGKAIINAKSDELFAGLPAEQTVWMSHSDKVIEIPEGFEVIADSPSTDYAAIE
                     DKKRRIYGVQFHPEVRHTEYGNDLLNNFVRRVCDCKGQWTMENFIEIEIEKIRQRVGD
                     RRVLCAMSGGVDSSVVAVLLHKAIGDQLTCIFVDHGLLRKGEGDMVMEQFGEGFNMNI
                     IRVNAKDRFMNKLKGVSDPEQKRKIIGNEFVYVFDDEASKLKGVDFLAQGTLYTDVIE
                     SGTKTAQTIKSHHNVGGLPEDMEFELIEPINTLFKDEVRKLGIELGIPEHLVWRQPFP
                     GPGLGIRVLGEITEDKLEIVRESDAILRQVIREEGLEREIWQYFTVLPNIQSVGVMGD
                     YRTYDHTVGIRAVTSIDGMTSDFARIDWEVLQKISSRIVNEVDHVNRVVYDITSKPPS
                     TIEWE"
     gene            complement(20190..21656)
                     /gene="guaB"
                     /locus_tag="P6L01_000381"
     CDS             complement(20190..21656)
                     /gene="guaB"
                     /locus_tag="P6L01_000381"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDP3029477.1"
                     /translation="MWESKFAKESLTFDDVLLIPAQSDILPKDVDLSVQLSDKVKLNI
                     PVISAGMDTVTESKMAIAMARQGGLGVIHKNMGVEEQADEVQKVKRSENGVISNPFFL
                     TPEESVYEAEALMGKYRISGVPIVDNKEDRNLVGILTNRDLRFIEDFSIKIVDVMTQE
                     NLITAPVNTTLEEAEKILQKHKIEKLPLVKDGRLEGLITIKDIEKVIEFPNAAKDEHG
                     RLLVAAAIGISKDTDIRAQKLVEAGVDVLVIDTAHGHSKGVIDQVKHIKKTYPEITLV
                     AGNVATAEATKDLFEAGADIVKVGIGPGSICTTRVVAGVGVPQITAIYDCATEARKHG
                     KAIIADGGIKFSGDIIKALAAGGHAVMLGSLLAGTEESPGATEIFQGRQYKVYRGMGS
                     LGAMEKGSNDRYFQEDKAPKKFVPEGIEGRTAYKGALQDTIYQLMGGVRAGMGYTGSH
                     DLRELREEAQFTRMGPAGLAESHPHNIQITKESPNYSF"
     gene            complement(21694..22962)
                     /locus_tag="P6L01_000382"
     CDS             complement(21694..22962)
                     /locus_tag="P6L01_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDP3029478.1"
                     /translation="MKNLILSVQHLLAMYAGAILVPIIVGTSLKFTPEQIAYLVTVDI
                     FMCGVATFLQANKVTGTGLPIVLGCTFTAVAPMILIGQTKGIDVLYGSLFLSGILVII
                     IAPFFSHLVKFFPPVVTGSVVTIIGINLMPVAMNYLAGGQGAKDYGDVKNILLGLMTL
                     IIILVLQRFTTGFIKSIAILIGLVLGTIGAGLLGMVDINQVNHAGWLGIPVPFRFSGF
                     SFDVTSTLVFFIVAIVSLIESTGVYHALSEITGKKLERKDFRKGYTAEGLAIVLGSIF
                     NSFPYTAYSQNVGLVSLSGAKKNNVIYGMVVLLLICGCIPKLGALANIIPLPVLGGAM
                     IAMFGMVMAYGVSILGHIDFKNQNNLLIIAVSVGLGTGISAVPQAFKGLGEQFAWLTQ
                     NGIVLGAISAIILNFFFNGIKYKQTEENVK"
     gene            complement(22962..23540)
                     /gene="xpt"
                     /locus_tag="P6L01_000383"
     CDS             complement(22962..23540)
                     /gene="xpt"
                     /locus_tag="P6L01_000383"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDP3029479.1"
                     /translation="MELLGQKVKEDGVVIDEKILKVDGFLNHQIDAKLMNEVGRTFYE
                     QFKDKGITKILTIEASGIAPAIMAALHFDVPCLFAKKAKPSTLTDGYYETSIHSFTKN
                     KTSTVIVSKEFLSEEDTVLIIDDFLANGDASLGLYDIAQQANAKTAGIGIVVEKSFQN
                     GHQRLEEAGLTVSSLCKVASLEGNKVTLVGEE"
     regulatory      complement(23771..23873)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            24053..24460
                     /locus_tag="P6L01_000384"
     CDS             24053..24460
                     /locus_tag="P6L01_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDP3029480.1"
                     /translation="MADITVVNDTGELYNVINQKKSEGYLESELTIISKSKLHLNDLP
                     DSEISLISTSGTFSDRMTKLLTGEDGEHAVLSRYNLAPDELEKYKQLILDDKMLVVAV
                     RDKSSHKEVQEHNSAYEEIDITHFAEASKGPKA"
     gene            24603..25265
                     /locus_tag="P6L01_000385"
     CDS             24603..25265
                     /locus_tag="P6L01_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029481.1"
                     /translation="MEFKTIQSVKDPHYNEALKLYDTKLNLSLTEDKRIIKRSLENNK
                     TENDYVFIVGLENDVVVSLATAHYEATTNSAFLIYLFAKDLPNHDELMTQTLHQIEHE
                     LNLLSNLVHSRSINFLMMEVPKEPADLTQDNGLIIEHRRQFLYEHQFERQTEIDYIHP
                     TNDGHTKKVDLFLKKNIKLTKDIYGTSVKSNYILKYVFANGIAREIIYALLEEMDLRK
                     PF"
     gene            25383..26339
                     /locus_tag="P6L01_000386"
     CDS             25383..26339
                     /locus_tag="P6L01_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029482.1"
                     /translation="MLTKEFAQRVELSEKQVRKIVQHLEERGYQLSKTEYRGREATDF
                     KEEDIELFKDIADKVKQTNSYDLAFDELEKEKDFLQVIVKNDDKNLPTNQNVAQLVED
                     LRLEIQKMREERHLLGQMMNQVHQQQQELKELQNQLTSKIDSNSESLKAIQTSQEAIQ
                     EAQASQAKVLAESTNKVEKNAVTEDKADSKDSKVAGVNTSTDAKTDTKAENAGDGTAT
                     KVDKEDQISATEAIEKASVEQSKNENAAETSNKEATVDADAQHDAEQQVAEAHAEASK
                     QATSNDSLEAKAENDSTASQSEMSEPKPQEEKKGFFARLFNL"
     gene            complement(27134..27232)
                     /locus_tag="P6L01_000387"
     CDS             complement(27134..27232)
                     /locus_tag="P6L01_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029483.1"
                     /translation="MQLNVNMMKFDRWHKGICKQIVLNLVEKGEKG"
     gene            27317..28705
                     /locus_tag="P6L01_000388"
     CDS             27317..28705
                     /locus_tag="P6L01_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDP3029484.1"
                     /translation="MNAFLTLINIIVLVIFIVILHMMARKHISFAKRVFTALGIGIVF
                     GVLLHLVYGTHSNVITSTSDWFNIVGQGYVALLQMIVMPLIFISIVAAFTKIQIGEKF
                     AKIGSLIFIFLIGTVTIAAIVGVVYALVFGLDASTINLGNAEQARGSEIAKQAKDLTA
                     HTLPQQILELLPKNPFLDFTGQRATSTIAVVIFASFIGFAYLRVARKQPDHGELLKRA
                     IDAIYSLVMAIVTFVLRLTPYGVLAIMANTLSTSDFGAIWTLGKFLIASYAALITMYI
                     IHLIILSLLGISPIRYVKKTLEVLIFAFTSRSSAGALPLNVQTQTRRLGVPEGIANFA
                     ATFGLSIGQNGCAGIYPAMLAIMVAPVANVEIDLQFIVTLIAVVIISSFGVAGVGGGA
                     TFASILVLSTLNLPVALAGVLISVEPLIDMGRTALNVNDSMLAGTGTAKLTKHWDKDT
                     FESNDNAALTSH"
BASE COUNT         9129 a         5130 c         4172 g        10312 t
ORIGIN      
        1 atgtttatca attaaatgct ttcttaattt aaaatcaagt tcttttaatg agatttcttc
       61 tttatagatg ctatagtatt ctgaagtcac agaagtagtg tgttgacctg tactcttaga
      121 aactgacaaa ttaacgtttt gaacagtatc tttatgttga gtcccatttg tctttgtaat
      181 tccaccaatc gatgttgtga tagctgattt gtcactacct tctcttacta caaacacgtc
      241 aacattagaa acttcgtcat catctttaac agttaatttt tcatttcctg tcaatgtaac
      301 atcaatttgt tgagaattat ctataaatgt aactttattt ccgtatttat atcctgttac
      361 atttgttaat tcaaatcctc tccctttata atattcactc aagtcttgag tagcttgtga
      421 tctaacctct aatgtacttg cttttactgg ctgagcagta gttgtaatca tacctgttgt
      481 taaaatccct agtgctaaac ttgctttagc aatatttttt aatttcataa ttctatgctc
      541 ccaattttta gtctatttga tttattctat tacgcaatac gaacaatcct cattcattat
      601 aatgagcaat ggtcgtgctt cacattaaac ttactttaac taaaaattaa tcattattaa
      661 gtaatttaat aaatagttaa ctaatatcat tcaattccta tcgatctact ctcttttatt
      721 tacgaataac actttatctc aacttaatct ttatttaact acttattctg tacacaattt
      781 cgacacaaaa aagacactgc gctttcacag tgcctaaatt taatgttcgt ttttaattca
      841 ctttactttt acaactttta tgttaaatta tacaaatttt attcttatgc aatctatgta
      901 tttatcaaag ttataagaac attttttgta aaaaggattg tttctctttt tctaatattt
      961 caattttcat tttctgttta cttatcaata tatccatttt tttaaagaaa tcacctatct
     1021 tttcttgttc ctccaataca ggtatatcta tatttatatt ttttaattgt ttatatttta
     1081 agttccatgt atctgatgtt aatccttgtg aattaatttt aaatttatga atcattctat
     1141 gtgttttaaa cttatatcca ataaataatg agctagtatt ttgtgttgga taaagcacag
     1201 tatatgcagg gctaacaatc ccattataat ttgatttacc actagcccct tgccacattc
     1261 tcatagaatt atatgcaata tcatttttcc taactacttt ataattactt ttatctttac
     1321 ttgaattatc ttttctatcc aattcactaa attttataat gccactattt atagttactg
     1381 aaagcatttg ccctttgtca gaacgttcgt ttctctcttt taaatatttt tctattttgc
     1441 tattttccca atctggataa tcttcaccat tctcatcttt gaatcgcagt tcctgtgaga
     1501 aaattttctg catatagcct tttttctgtt gttgaagtaa ttcaagcttt tgttcttcta
     1561 attcaatttg tcggtcgagt ttgctgaaga acttgcctat tttttgctgt tcttctaaac
     1621 ttggatattt aattagaata gtaaaaaaat cattcacaga aacatttaat aatccgtgat
     1681 ttcttgcacc ctcaactgca attccagaaa cttctctata ccagtgtgtc gaatcaaaat
     1741 atgcttccat gaagtcttta gacatttcac ttttaataga aaaacaaata tacaaagagg
     1801 acaatacacc actatcatat ctagttaatc ttttaatagc ccctaatggg tatccattag
     1861 aataactttt gttatacgcg aattctccat tctttattag tgtataattt tctagatttt
     1921 tcgacgaaac tgatttacta aaatattctg tttgatcaat taaacctaac tgtccggata
     1981 ttgttaaagg ctttttcgat tctaagtttt tatttttcct aattactcta tctgtaagat
     2041 cccccaactg cttctcttcc cattcgcctt caaaccctgg gaatctcaac tctggcacat
     2101 ttttcttttg tgtattactc atctttcaac accccaagtt ctttcaggta tgcattgatt
     2161 tcttgttcaa tttctgcgat ttctttgtcg atatttttca aatcttgttg gacttgatct
     2221 aaatcaattg gcgcttcttc ttcgaatgta tcgacatacc tcggtatgtt taggttgtaa
     2281 tcgttatcgg caatctcttg taatgtcgca ctgtaactgt atttatcaat tgtttcttta
     2341 cgcttgtatg tgtcaataat acgttcgact tgggcatcgc ttaaatggtt ttgatttttt
     2401 cctttttcaa aatcattgga tgcatcgata aataatacgt tgtcgtcttg ttggcgacat
     2461 tttttaaata ctaagataca tgttggaata cttgtcccat agaaaatatt cgctggtaag
     2521 ccaatcacgg cttctaagta gttcttttct tcaattaaat aacgacgaat gacgccttct
     2581 gcggcaccac ggaataagac accatgtggg agtacaactg ccatggtacc ttcatcgtct
     2641 aggtaatgta ccatatgttg aataaaggca aagtctgctt tggattttgg cgcaagcttg
     2701 ccgtaaccac tgaagcgttc atcattttca aattttgaat ctgcagtcca tttcgcactg
     2761 tatggtgggt tcgctataac cgcatcaaat gtattgccta agaaagctgg attttccaac
     2821 gtatcatcat tacggatatc aaagttttca tagcgcacat catgtaataa catgttcatg
     2881 cgcgctaagt tgtaagtggt attgttacgt tcttgaccga aataacgata cacttgcgtt
     2941 tctttaccaa cacgtaacaa taatgaacct gaaccacatg ttgggtcata cacgtgacgt
     3001 aatttatctt taccgtctgt gacaatcttc gccagtatct tagatacttg ttgtggtgta
     3061 tagaactcgc ctgctttttt acctgctgtc gccgcaaagc gcccgataag aaattcgtat
     3121 gcatcaccta acatatcaat ttccatatca ctgtgaacga atggtaagtc gtcaaggtta
     3181 accataactt tggaaattag tgcagtacgt tctttgacat tgttacctag tcgcgttgaa
     3241 cttaagtcca tatcgctgaa cagtccgata aagtcatttt cactttcttc acctagtgtt
     3301 gatgtttcaa ctttacgaat cgccgttgcg agatgttcga tatcgaaatc ttgcgtttca
     3361 atttcacgaa tcatcgcact gaataaatct tgtggttcaa tgaagtaacc gacttgatca
     3421 attaattcag cttttaagtc ttcacgatat tcttcatctg cccatgcttc ttgatacgta
     3481 atatcttcac ctgacaaggc atctgcatat tcttgttccg ctttttcaga taagaagcga
     3541 tagaaaatca agcctaaaat gtaattacgg aattcactcg catccatatt ccctcttaaa
     3601 tcattcgcaa tcgaccataa ttttttatgt aattcagctt gttgctgacg ttgtttttca
     3661 gtaatagaca tgtgattcct ccgcctttga ataagtaatt tatctctttg tgtaatagat
     3721 ttattataac atttggttat gttgcgatgt ggagaatttt gatgttggtg gtggaatttt
     3781 tatattcatg gagattggga taggattcat gacacacaaa caaaaacggc ttaacgacga
     3841 tatcgattga tgcatccttt ttgtataagc tatgcgcacc aatttacatt tcaccgttaa
     3901 gccgcttcaa ttacttttta ttattcgtaa ttatttactt taagttaact tcaatatctt
     3961 taatttgttt gctttctatg acttccccca tatatttaaa ttttaattta gatcttaaat
     4021 caaggttata aaaactgcca tctttcaagc taattttgac cctaccatct tttgagattg
     4081 ttttataaag tccgtatttt tcaattagat gctttctcaa cttataatcc agctctttta
     4141 gtgttatttt ttctttttta atgtaataag ggtaaccttt cacaaaagca tcttcaccct
     4201 tttcttttga aatatttaat attggtgcac tgacaactcc agatgtttta ttattctgta
     4261 ttacaccacc aacagtataa aatatatcgt gcttatctct tttttcttga acaaaaaaca
     4321 catctaaccc ctcataacta cgctgttgaa atttactttt atcatttcca ggcagtaaaa
     4381 cttgaatctt aatacctcta aacttaaaac gcaagttatt tttaccatgt ttcaaagcac
     4441 taatattttt catttccata gtctttccag tgtagtatcg gtatagtgcc tccttgtcat
     4501 gtttatttac tgacttttga ttttgttttg catgacctga atgaacttct gttgttaaag
     4561 ttcctgttgt taaaattcct aaagctaatg ttgcttttgc taatgctgta aatttcatat
     4621 ttatttgctc caatcttaat atattggatt gttttcatta cgtaatttga atcatacatt
     4681 tttattataa cgtgcatcgt ttcatacgat aattaacctc gatttaaata tacttgaatt
     4741 attcttaatg aaaaacaaat atattttaat gttcaggtta aatgactttc attatttcat
     4801 ttagttgttt tactggatta tcgtttacta caactgatga tgttactctg ttctgctctt
     4861 tgaattacaa aaacaactta acaacagtaa tgtatttaac acagttcctt atataaggac
     4921 acagatgtca attcacattt tgtcgttaag tcgcttctta agctattatg ctattaactc
     4981 aaactaattc aaattcactt caatattttt aatttgctta ctatccatta catcaaacat
     5041 acgatcaaaa cttaattttt cacttaaatc aattacatat ttcttttcgt ctttcatatt
     5101 aataacgatt ctacctttat ctgacgcgcc tttatacaat ctatattttt cgactaacat
     5161 ttttcttatt ttaaaatcca gttccttcaa cgatacttct tccttttgaa taaagaacaa
     5221 ctcatttatc gaaaagccat gtttagcatc aactttttta acttgtagac ttggattact
     5281 tacaaatcca aacactgatc tcacattttt ctttgttata ccgccaacgc tatatatgcc
     5341 accttttata tctattaatt caggtactgc aaagacatca aggccatgtg ttttttcttt
     5401 atatttattt ttatcgtcac caagcagaaa cactttgaaa gttttatttc gttggttaaa
     5461 gtttaaaacg ttagaaccat agtaatcttc acttttaacg ctaatgtttg ttgattcaaa
     5521 actttcttca gaatagtatt gatgtagcat attgatataa tatttgcgtt gtgtctcatc
     5581 aagttctact ttcgcgtgaa cagctttact ttctgctgta aacacacctg tagttaatat
     5641 tcctaatgct aatgttgctt ttgctaatgc agtcaatttc atatttattt gctccaatct
     5701 tgatatattg gattgttatg attacgtaat ttgaactatc tgtctttatt ataaggtgca
     5761 tcaaaacaga ctgacattaa cgctcattta aataaacttg aatcattatt aatgaaaatc
     5821 aaacgtattt taacattcgt attaactgat ttttaatagc aacataaatt tttttggggg
     5881 aatcattgtt ctttccgatt agttttagat cttaagttat tcgatacaaa gtgtacaaaa
     5941 aagcagctta ataacgtagt caatgttcga tatgttttta ttattaaaac acgtatcaaa
     6001 atcattaaac atgggattat taaaccgctt cttaagcttt tattctatat atcattgatt
     6061 atttcaaatt cacttcgatg ttcttaattt gttcactatt aatgacatct gccatacgct
     6121 cgaaatctaa tttatcactt aaatcaattt catacttatt ttcatctttc atattaataa
     6181 caattctacc tttatcagct gacccttcat acagtttgta ttttttaatc aacagttttc
     6241 ttattttaaa atcaagttcc ttcaatgaca cttcttcctt ttgaataaag aaaaattcat
     6301 caatagaaaa accgtcttta tcgtctattt ttttaactag taagttcggc gttcttagag
     6361 actcaaatat tgattttacg tttttcttcg ttacaccact aacactaaat attcttccat
     6421 ctaaatctac taattctggt accgcaaaga catctaaacc atgtgttttt tctttgtatt
     6481 tattttcatc ttttcccaat aaaaatactt ggaacttttg attttgttgg ttaaaaagca
     6541 aaacgttaga gtcataataa ccttgacttc ggccactaac atttgttaac tcataactag
     6601 gtcctgaata gtattgatgt aacttgtttg tatcatataa acggttcatt ttttcatact
     6661 ttccttttgc attaaccgat tgattttctg ttatcattac acttgttgtt aatattccta
     6721 atacaaatat cgctttagct atcgctgtaa atttcatatt catatgctcc aatcttaata
     6781 tattggattg cttttattac gtaatttgaa taatacacat ttattataaa gggcattgag
     6841 acagactgac attaacactc gtttaattaa aattaaatca tggttaatga agaactaatt
     6901 aatattcaat ggacatcact ttcagaagta atgtgaattt ttagtacgct cataccctgc
     6961 gttataaaac ataaaaacag cctaacgaca ctgttgattt agatacgccc ctttttggag
     7021 gatgtacgta tttattcaac atttcatcgt taagctgctt caaatttatt actttaaagt
     7081 cattgattgt ttaatttaaa tttgattaat agtcactgct atattttgaa tatccttact
     7141 attcaacaca tcacccatgc gctcgaattg cagtttatca cctaaatcaa ttacttcctt
     7201 tttctcgtct ttcaaattga tagtgatctt accgtattta gtcgtacctt tatataaacc
     7261 atatttttca actaattgct ttctaatttt gaaatcaagt tctttcagtg aaacttcttc
     7321 tttattaatt aaaaatgagt caattgatgc atctaaattt ccgccataca ctttattaac
     7381 aaataaatgt gtattagttt cagaagattt gttatttttc ttagtcacac caccaacagt
     7441 agatagtcta ccgtttggat caattaattc ttttaccaca aagacattct ggccttcaag
     7501 gcctttttta tatttatctt tatcttttcc taataagaat aattggtgat tttgtttttc
     7561 ttggttaaag cgtacaacgt tagaaccgtt atagttttca accttaccac taatattact
     7621 gaattcaaaa ctttctgatg agtagtatcg atataagtct ttaatatcat ataaatgttg
     7681 tactctctct tgcttttctt ttgcgtggac tgcttggcct tctgatgtaa tcacaccagt
     7741 agttaataag cctaatgcca atgttgcttt agctaacgtt tttaatttca tagtactatt
     7801 ctcccaatct atttataaat tttgtcttaa tatattttta tatgattaat tcaattacgt
     7861 aatacgaaca atctactatc attatataag cgagatactg cgctatgaat taacctcttt
     7921 ttaactattt ttgaaccaac gttaagttag ttaatgaaat cctaacagta ttaattattt
     7981 tttataacac aatgctaatt ttttaactat tcacactttc cctcaattta attatgtttt
     8041 ccacattgtt tcatgtcact aaagcgacat cacgcatcta taagtatcaa ctatttccac
     8101 aagttttatt ggtgttttta ttattcatcg atacgcttca ttttcatctc gccaacacaa
     8161 aaaagaagct aagcaactta tgttgcctaa ctcctctata ctatccatat tttactatta
     8221 tccatatttc attgaattat ctaatgttgg cttctatttt ttcaatattt ctaccgtcaa
     8281 tgacatcact catgcgattt gtttgtaatt ttttattaag ttcaaacgta taatagccgc
     8341 catctttcat tatcactttt atcttactat ctttaggaaa ctttttatac agatcaaaat
     8401 tttgaattaa atactgtctc aatttaaagt cgagttcttt aagtgaaatc tcttctttat
     8461 aaatgtagtg tactctaccg tacgtagcaa taccgtcacc ttcatctctc ttgatttgaa
     8521 atcttggtgc gtttatataa tcataataag cgtcttgatt tttcttagtg acaccaccat
     8581 atgaaaacac tgtgccgttg cgattttctg cttctttaac aacaaatatg tctaatcccg
     8641 gatttttgcg tgctttaaat ctttcaatat ctttaccaaa tatctgtact cttgtgaatt
     8701 ttctattttt atcaaagata aggtaatgct tgccaccttt gctataacga taaccagtaa
     8761 catttttaag ttccttactt gcgccactat agtaatctct taagtcaaag atatcttttg
     8821 tcacattttc atattttgct tcatgttcac tcgcatttac agtttgatgc aatgacgtta
     8881 ttgttcctgt tgctaaaata cctaatgcta aacttgcttt cgcaattgct gccattttca
     8941 tagttgtatg ctccattcgt aattattaga tttgttcgct tacgtctatt gaatcataca
     9001 gctttattat aattggcgta tttgaccttt cacattaaac catctttaat aatcattgaa
     9061 tcattattaa gtaaattaag gaatctataa tgttcgttaa ataaaactga ttccgttgtg
     9121 cttcacaccc gatagatagg gatttacaga taaattcagg actcttccac gtcatatttg
     9181 gacccatcga aaattcgggt tctcaaatca tcgaacataa caaaagaagc taagcaacat
     9241 gtaggccgtt gtcactgaac ttcttgtttt tccgatgaca gcttctattt agagaatgtc
     9301 atgattattt tatattcact tcaatgttat caatattagt gccatctatg acgtctgcca
     9361 tgcgattttc ttgtaatttt ttgtgtaatt caaacgtgta ctttccaccg tttttcatgt
     9421 taataacaat tttacctgaa ccaacgttac cgtacagatt atgattttca ataagttgtt
     9481 ttctcaattt aaaatcaagt tctttcaagg aaatctgctc tttagtaatc ttgaattctg
     9541 aaacatcatg agagattgta cctttattat cttctttagt aattcttact cctgctttgt
     9601 gatcaacttt tttactatta ctctttgtga taccaccgac cgagtatctt tccactccat
     9661 atttattttt tcttctaaaa cgacaaatac atcgacattc ctatgtactc cttcatcata
     9721 ttttttatcg tcttttccaa ctaaagctat tttatatatg aaataatctg gaacaatatt
     9781 cataaatctt attgtcgtcc atttttttaa aataatacca atctcatttt taaattctaa
     9841 acttggtttc gtataatacg ctcttaaatc tttaaattta ggatttattt ctgttggtac
     9901 ttgttttgtg gttggcgatt gtggtgtttc cactttagtt gaagatggtg ttgtcgcgtt
     9961 cggtgtttgt tgcggtgttt ccactttagt tgaaggcggt gttgtcgcgt ttggttttga
    10021 ttgcggtgct tctactttag ttgagggcgg tgttgattgt ggtgcttcca ctttagtgga
    10081 agatggtgtt gtcgcgtttg cttcttgcgt tgtcgttgtg attacacccg ttgttaaaag
    10141 gcctagtgct aaacttgttt tagcaattgt tgttattttc atagttgtat gctccattcg
    10201 taattattag atttgttcga ttacattcat tgaatcatac agctttatta tagatggcgt
    10261 attgctccat tcacattaaa ccttgtttaa ctatatttga atcatcgtta agtaaattaa
    10321 gaaatccata atgttcgtta aataaaactg attttgatgt gattcaacac ttggcacatt
    10381 tgaggattcg tcacttttaa gacatagaaa tgccactttt acaaacaaat gaatattcgt
    10441 cttttacatc attacgcata ataaaagaag ctaagcaaca tgtaaaccgt tgtcacttaa
    10501 cttcttgttt taccgatgtc agcttctatt tagagaacgt catgattatt ttatattcac
    10561 ttcaatttta tcaatgtttg taccttctat gacatctgcc atacgatgct cttgcagttt
    10621 tttgtgtaat tcaaacgtat acttcccacc gtttttcatt ttaataacga ttgttcctga
    10681 acccatgtta ccgtaaagat tatgtttttc aataagttgt tttctcaatt taaaatcaag
    10741 ctctttcaag gaaatctctt ccttagtaat catgtattct gaaacatcgc gtgaaatcat
    10801 accttgatta tcttttttag taacgcttaa ttctgctttg tgatcaactt ttttactatt
    10861 agtcttcgtg atgccaccga cagaatattt ttttaattga tatttattgt cttctaaaac
    10921 gataaataca tcgatattat cgtaaggtcc atctttatat tttttctcat cttttccaac
    10981 taaagctatt ttatagatga acctatttgg aataacattc ataaacctaa ccgtcgtcca
    11041 tggtttgagc aaaaatccaa actgcttttc aaattcaaaa ctcggtttcg tgtaatacgc
    11101 tcttaaatct tcatatttag gagtcatatc tgtttgtgct tgttttatgg ttggagattg
    11161 tggtgtgtct gatttagtag attgcattgg ttgtggcgtg tttgttgatg gaggtgttgt
    11221 cactttagtt ttcggcgttg tggattcggt tgtcgtttgt gattgttctt gtttaggcgc
    11281 tggcgttgct gatatattaa gcgatttctg ctcttcttgt ttaggttgtg atattttttc
    11341 tattttggaa gctgaggttt tttcctcatt agtatttggt gccttttcga gtttaggcgt
    11401 gcgttcttgt cttgtgttag ctgcttgtgt tgtcgctgaa tttgcacctg ctgttatgtt
    11461 tatcattgct aatcgctctg ctttaagcgt tggtactttg tcaactttag ttgattgtat
    11521 tttttctgct ttgaccgatt gcgtcgttac tgtaattgcg cctgttgtta aaagccctag
    11581 tgctaaactg gttttagcaa ttgttttcat attcataatt gtatactcca atctatatta
    11641 tattcgattg tctttttacg taatttgaat catacaacat cattatagat ggcgttctaa
    11701 gataatcaca ttaaaccctt tttaacaatt attgaagtat tattaagtaa tttaagcaaa
    11761 aaataatgag tgattatgag gttaatatag cgtttctatg tgcctttgaa atgattttta
    11821 agcattaaaa agaagttaag caacgtttga tcgtcactta acctctctat ttcaatttca
    11881 aattatttcg tcgtcaagta tgtgtattaa gcttttctaa ctttgatttc aattctatca
    11941 atatctgtga cattgataac atcggacata cggtcttctt gtaacttttt atccaattca
    12001 aatgtatact ttccatagta tttctttttg acggtaattt tccctgtact catttcaccg
    12061 taaagaccat aattatcaat aaggtatttt cttaatttaa aatcaatctc tttcaatgac
    12121 atcgcttctt tatctatttt aaatgggaaa aagtcataat catattcacc aatatgatct
    12181 gctttaataa ctcttgcttc tgctattagg tcgacagctt tatcgtttgc actagtgata
    12241 cccccaatag agtactttgc accttcaaat ctcttatcct cattaacgta aaatatatta
    12301 agattacgat gtacacccgt atgataatgt tgcttatctt tgccaattaa agcaaggtta
    12361 ttaacagaat taccatctat gatattcata aatttaatac ttggttgaat gaaactgata
    12421 taacctgtca catttttata ttcaatacta ggttgagtat aataagcttt taattttttg
    12481 ctattttcac ttattacaat aggtttcttt tcggcatgaa ctggtttttc cgttgtagtg
    12541 tttacacctg ttgctaatat tcctaataac aaacttattt ttgcaatatt tttcattttc
    12601 atagttgtat gctccaatct attataatta gattgtttta ttacgtaatt tgaaccatac
    12661 acccatatta taggagctgt attcggatat tcacattaac ctgtttttaa ctattcataa
    12721 aatatgatta agctatttaa gcaaaagata atgcgtggtg cgactgtttg tgtgagtttt
    12781 gtaacttttc ccctcaaaaa aagaagctaa gcaacagtac gtcgcttaac ttccatatat
    12841 ttccataaca agcactattc aacatagcgc tttgttgtta gaaagtatta tttcatttct
    12901 actagaattt tttgtatttg tctgccgtcg atagaatcac ccatacgttc tttttcaagt
    12961 ttttgactta aatcgatagt atgtgatttg ccatctttca ttgtaattgt aatttgacct
    13021 tgtttaagac catttgaata caagccgtgt tgtttgattg cacgttctct taatctataa
    13081 tcaagttctt tcaatgagat gtcttcttta taaatttggt ataaactact ttgtggttct
    13141 tctttacctt tcttgatttc aaggattggt gtgtgtatat agtcaataaa aggttgactg
    13201 tttgtttttg ttacgccacc aattgagtaa tttgttgctt gtctactgtc accttctcta
    13261 aggataaaca catctatatt cgagttgtct ccatctttaa atttgtcttt atcagatcca
    13321 actaaagaaa tttgagaata ttgattgtct actataacat ctatataatc tttacctttt
    13381 tcagtatatt tatatccagt aacattttta cgctctaaaa ccggtttatt ataatagtgt
    13441 ttcaactctg attcactttg ttgtttaact tctgttttcg cttgtactga ttgtacattc
    13501 gatgtaatta cacctgttgc taacattccc aatgctaaac ttgcttttgc tatcgcttta
    13561 aatttcataa tttttagccc caatctattt aaatttgatt tttcaattac gtaatatgaa
    13621 caatctacct acattatatt gaggcgctat cccctttcaa attaaacctc atttaactaa
    13681 acttgaatca ttgttaaggt aattaataaa atgctaactg ttattaatca attcttatcc
    13741 gtcgagactc ttttttatac aaatcacact ttataccatt tttattttaa aaactgtaaa
    13801 actttcagac acaaaacaga catcatattg tcgtagtacc tgattaaaat tttcaaagat
    13861 attagtgata cacatcaact aaaacgcgtc attacggtat tttcaacatt aataacatat
    13921 acgtatttac tatacacagc gataataaat acagattaca attttttata aaatcaaatg
    13981 ttttgaaaac aatcgaataa gtattatgtt atgtatataa agattaggag atgaagtgaa
    14041 tgaatattat gttaacaggt gctacaggtc atttaggcac acatattaca aatcaagcca
    14101 ttgcaaatca catagatcat tttcacattg gtgttagaaa tgttgagaaa gttccagatg
    14161 attggcgtgg aaaagtttct gttcgacaat tagattattt taatcaagaa agcatggtag
    14221 aagcatttaa aggaatggat accgttgtgt ttattccaag tattatccat ccatcattca
    14281 aacgtattcc tgaagtggaa aatttagtat atgcggcaaa gcagagcggc gttgctcata
    14341 tcattttcat aggttattac gcagatcagc ataataatcc attccatatg agcccttatt
    14401 tcggatatgc atcacgttta ttgtcaacaa gtggcattga ttatacgtat gtaagaatgg
    14461 caatgtacat ggatccactt aaaccctatt taccagaatt gatgaatatg cataaactga
    14521 tttatccagc tggtgatggt cgtattaatt atattactag aaatgatatt gctagaggtg
    14581 tcattgctat tattaaaaat ccagatactt ggggcaaacg ctatttatta tcaggctata
    14641 gttatgatat gaaagaactt gctgctattt tatctgaggc atcaggcaca gaaattaaat
    14701 atgaacccgt ttcattagag acatttgcag aaatgtacga tgaacctaaa ggctttggtg
    14761 cattattggc atcaatgtac cacgcaggag caagaggact attagaccaa gaatccaatg
    14821 atttcaaaca attagtcaat gatcaaccac agacactgca atcattttta caagaaaata
    14881 tttaataata aaggagcgtt acagtgaata tcatctcaac aattttaatc atatttatgg
    14941 cattagagtt tttctatatt atgtaccttg aaacgattgc tacaacttcc aaaaagacta
    15001 gcgagacatt taatataagc gtcgataaat tgaaagacaa aaatattaac ctacttttga
    15061 agaaccaagg cgtatataac ggtttaatcg gagttttgct aatatacggt ttgtttatca
    15121 gcagtaatcc aaaagaaata tgcacagcta ttttagtgta tatcattggc gttgctattt
    15181 atggtggcct ttcaagcaat attagtatct ttttcaaaca aggcacattg ccagtattgg
    15241 cactcatatc aatgctttgg taagtattgg tgtttggggg tttggagatg tagtctgggg
    15301 ttgagagttt ggagggtttg agtgttttgg gtgtggacgt tcataaactc agagtatttc
    15361 atcctaatta tttcaagcag aggtgacagt agcgtttcct ctgtttcctt atagaaaaat
    15421 gatttaatga agaaaaccca tatctgtttt aatttttagc tgataaatac tccatatatt
    15481 agaatggcta ctttatctat tgcatcaatc ctttaaaaca aaaaacccat gatttcgaaa
    15541 ttcccgtatg gtggggttcc tactctcatg gatctattaa ataaattatc actataagtt
    15601 tattatttca atattattaa caatatatgt agtcgtaaaa ggaaagagga catgagaact
    15661 tcggtgttga ttggcattac ataacgcttc caaacatatt atttggtaac aataagaaac
    15721 tatttacaca acctattttg tatagtaaaa ttattttata agatttaaat ccaattgtac
    15781 aaggagtgaa tatcatggta ccagaagaaa aaggttctat tactttgtca aaagaagctg
    15841 ctatcatatt tgcaaccgca aaattcaaac catttaagaa cagaattaaa aataacccac
    15901 taaaaacaaa tccatttctt aaattacatg aaaacaaaaa atcttaataa ctttatttat
    15961 agcatttcta atctcagaaa tgctataaag gacaacgccc tctttcataa ctatataagt
    16021 caagaaacca agacatttca aacatcttta aacccttcaa tagcacattc cctaaaacat
    16081 aaagcaataa caatgaacga tcaaaattcc ttacgtactt atgatgtgtc agtgaatcgt
    16141 ctacacttaa ttgaacaaat tctatgagaa tagatattgt taaattaaga aagtagtcaa
    16201 tcttattatg acaaaagaaa gaagaacatt tagttcagag tttaagttac aaatggttag
    16261 attatataaa aatggtaagc ttaagaatga aattatacga aagtatgatt taaaaccctc
    16321 aattatctca aattcgataa aacaacacca aaatactgga cccttcaatc atcaagataa
    16381 tttaaaaagt gatgaaaaag agttaataaa attacgcaaa gaagttcaac atttaaaaat
    16441 ggaacatgat gttttaaagc aaattttaaa gttgatttag aaactgaact ttttaaagat
    16501 acattacttg aagattctaa tttatctgat tatataaaga accaacttcc aacgatatgg
    16561 agagactaag aaatagttta aatttattta atttcaataa aaactcataa taatatttaa
    16621 gtaagttaga ttgcttttag cattaggaat tcgcttataa ttagatgttc aatatagact
    16681 tttttacaca tacatgaact ataaaaagcg aataaataga aggtcattgc gaaatttgct
    16741 aaatacaaaa ttgatgagtt ccacctaata aaaattgttg gttttatagt aacatctatt
    16801 ttcgtaccat tcatgttaat ttcagtgcta ataatgctga tataaagaag aataaattga
    16861 ttaggagaac actatttaat gtctaaaaat atcaatcaag caaatagtaa attaaatacg
    16921 tcaaacaaaa aactaaaaca agcttattca aaatccgata gtaaaaacat gaaagttata
    16981 tacatgcctc attggctaga gttttattca attgctatac atagcgatgt tacaagcaat
    17041 aaaaaaagat attataaatc atttagtaga ggaacagtgg tatatgtaaa actagggagt
    17101 aatattggaa gtgagttttc tggtaatcat ttttgtgtaa tattagataa caaagacaat
    17161 aaaggaaaag aaacagttac aatagttcca ctttcttcca aaggtaataa aaattatttg
    17221 aagttaaacg aatcggtgct aaatctaaca gcaacagatt taaaaaaaca gattatcgat
    17281 atcagtagta aagtacaagc attaacccaa aaatatagag atattgacat taaacttagc
    17341 acagaagata aaaagctttt gtctaactta aatcaaaaag ctaatgaact gtatagagtg
    17401 ataggtgttt attccaagca caaagggaaa gatacatacg ttaatatatc agcaattaca
    17461 acaataagca aaagacgtat aagcaaaata aatgattctg atcctactgg aacgataata
    17521 atttcagaag atgacatgaa tgaaatcgaa aagcaattaa agataaagtt cttttctaat
    17581 tgaattgtaa tattacatct gatataattg atttacaaat tcccggtaac cattccggct
    17641 tcattctctt gaagaatgac atattctcag cgttttagct gaaggtcaga tgatacgtca
    17701 tctggcctct ttttttatat aaatgccctt taagtgaaat atacaacaat ctgcaaccaa
    17761 tatagacatc acaaaacaca caataaatta cacaatgtgt tataagccac atgtcattat
    17821 tatcgtagct agctgcgtca ctcaaaacga atacggaacg cgttggttat tccaatcata
    17881 atattactct gcaaatacac agccctgtat ccgagtaaat gaatagagat ttgaacaaat
    17941 gaaaacaatc aactaatgga aaggataaaa tattatgcac aacaaaaatg aattcaacaa
    18001 aaaactgaaa gattttatag gtagcgataa acggatggct ttagtaaagg gttatattaa
    18061 cgagtataaa ttaacaactg ttttaagagc tttaaataat tccgattaca caaaagggac
    18121 gatacacact cgttcaatag gcaacctaaa acaaatagta gataatcgaa ttattcctaa
    18181 aaatataact caaaacaaaa tcttcaaatt gagtaattta accttagagg ttaacctcta
    18241 tgaaagaaac acaatttatc acggagactt tgctatttac tatcctgtag aatctgctct
    18301 aataatagaa acagatactc ctaggctaat taatcatata aataacaacc cagtccaaaa
    18361 aatatttata ataacaacaa atgactggag ctttaataca cttgaattag aaaaaatagt
    18421 agacgaaact ataatttatg atttaaaaca agaagatcct aaacaatatg aaattttata
    18481 taaaaataaa cacggaaaat tgccttattg atgaattaaa acgcaaactt agaacacctt
    18541 accccttttc cgtccttatt gtaattttcc aactactcaa attaaaaacc acccgtgcaa
    18601 acaggtggtt ttattatata taattattcc cactcaattg tgcttggtgg ttttgatgta
    18661 atgtcataga ctacgcgatt gacgtggtct acttcgttta cgatacgact tgaaatcttt
    18721 tgtaagactt cccaatcgat gcgtgcgaag tcacttgtca tgccgtcgat agatgttact
    18781 gcacggatac ctactgtgtg atcatacgta cggtagtctc ccataacacc tactgattga
    18841 atgtttggta acactgtgaa gtattgccaa atttctcttt caagaccttc ttctctaatc
    18901 acttggcgta aaatcgcgtc tgattctcta acgatttcta gtttatcttc agtaatttct
    18961 ccaagtacac gaataccaag accaggtcct gggaatggtt gtctccacac taaatgttct
    19021 ggaataccta attcaatacc taatttacgt acttcatctt taaacaatgt attgattggt
    19081 tcgattaatt cgaattccat gtcttctggt aatccaccaa cattgtggtg tgatttgatt
    19141 gtttgtgctg tcttagtacc tgattcgatg acgtctgtat atagtgttcc ttgcgcaagg
    19201 aagtctacac ctttaagttt tgatgcttca tcatcaaata catatacaaa ttcattacca
    19261 atgattttac gtttttgttc aggatctgaa acacctttta atttattcat aaaacgatct
    19321 ttcgcattaa cacgaataat attcatgttg aacccttcac cgaattgttc cataaccatg
    19381 tcgccttcac ctttacgaag taagccatgg tctacaaaga tacatgttag ttgatcccca
    19441 attgctttat gcaataatac agcaacaaca gatgaatcta cgccaccact catcgcacat
    19501 aatacacgac ggtcgcctac gcgttgacga atcttttcaa tttcaatttc gataaagttt
    19561 tccatcgtcc attgtccttt acaatcacaa acacgacgta cgaagttgtt taataaatca
    19621 ttaccatatt ctgtatgacg tacttctgga tggaattgca caccataaat gcgacgtttc
    19681 ttatcttcaa ttgctgcata gtctgtgctt gggctatcag cgataacttc aaagccttct
    19741 ggaatttcaa taactttatc agaatgactc atccaaacag tttgttctgc tggtaagcca
    19801 gcgaataact catctgactt cgcattaatg attgctttac cgtattcacg ttcattggca
    19861 cgttcaactt taccacctaa taatttagta gttaattgca tgccgtaaca aataccaagt
    19921 actggaattc ctaaattata tatttccgga tcaattgtaa atgaaccttc ttcataaact
    19981 gaatttggac cacctgataa gatgatacct tttggattca ttttcttaat ttcttcaatt
    20041 gaaatttcat gatcgtgtaa ttcactataa acgcccattt cacgaattcg gcgtgtaatt
    20101 aattggttgt attggctacc aaagtctaag acaaggatta actcttgttc ttttgccatt
    20161 tccatatttg tcgttctcct ttatcttaat tagaatgagt agttcggtga ttctttcgta
    20221 atttgaatat tatgtggatg gctttctgct aaaccagcag gacccatacg tgtaaattgt
    20281 gcttcttcgc gtaattctct taaatcgtgt gaaccagtat aacccatacc agcacgcaca
    20341 ccgcccatta attggtaaat tgtatcttgt aacgcacctt tataagccgt acgtccttcg
    20401 ataccttcag gaacaaattt cttaggcgct ttgtcctctt ggaagtaacg gtcgtttgaa
    20461 cctttttcca tcgcacctaa agagcccata ccacggtata ctttatattg tctaccttgg
    20521 aaaatttctg tagcacctgg actttcttca gtacctgcta ataagctacc taacataacc
    20581 gcatgtccac cagcagctaa tgctttaatg atatctcctg agaatttaat accaccatca
    20641 gcaatgatag ctttaccatg tttgcgtgct tcagttgcac aatcataaat tgctgtaatt
    20701 tgtggtacac caacacctgc tacaacacgc gtcgtacaaa ttgaacctgg gccaatacca
    20761 actttaacaa tatctgcacc cgcttcaaat aaatcttttg ttgcttctgc agttgctacg
    20821 ttaccagcta ctaatgtgat ttctgggtaa gtcttcttaa tatgtttcac ttgatcgata
    20881 acgcctttag agtgaccatg tgctgtatcg ataactaaga catccacacc tgcttcgact
    20941 aatttttgag cacgaatatc agtatctttt gaaataccaa ttgctgcggc tacaagtaga
    21001 cgaccatgtt catcttttgc tgcattaggg aattcgataa ctttttcaat atctttaata
    21061 gtaataagac cttctagacg tccgtcttta actaatggta acttttcaat cttatgtttt
    21121 tggagaattt tttctgcttc ttcaagtgtt gtattcactg gagctgtaat taaattttct
    21181 tgcgtcatta catctacaat tttaattgag aagtcttcaa taaaacgtaa gtcgcggttt
    21241 gttaaaatac ctactaagtt gcgatcttct ttattatcaa caattggtac acctgaaata
    21301 cggtatttac ccattaatgc ttccgcttca taaacacttt cttctggtgt taagaaaaat
    21361 gggtttgaaa tgacaccatt ttctgagcgt tttacttttt gaacttcgtc cgcttgttct
    21421 tcaacgccca tatttttatg aataacacct aaaccacctt gacgagccat agcaatcgcc
    21481 attttagatt cagttactgt atccatacca gcagaaataa ctggaatatt taatttaact
    21541 ttgtctgata attgtacgct taaatcaacg tctttcggta aaatatcaga ttgtgctgga
    21601 attaataaca catcatcaaa cgttaatgat tcttttgcaa atttactttc ccacattaaa
    21661 aacagcctcc atttttcaaa ttaattagtt atattatttc acattttctt ccgtttgttt
    21721 atactttatt ccattaaaaa agaaattaag aataattgca gagattgcgc ctaaaacaat
    21781 tccgttttga gtcaaccatg caaattgttc acctaaacct ttaaatgctt gtggtacagc
    21841 gcttatacca gtacctaatc ctactgatac agcaataatt aataaattgt tttgattttt
    21901 aaaatcgata tgtcctaata tactaacacc atatgccatt accatgccaa acatagctat
    21961 catcgcacca cctaacacag gtagcggtat gatatttgct aatgcaccaa gcttaggtat
    22021 acaaccacat ataagtaata acacgaccat gccgtatata acattatttt tcttagcgcc
    22081 ggataaagaa acaagtccta cattttgcga ataggctgta tacggaaatg aattgaatat
    22141 agaacctaac actatcgcta gaccttccgc agtataacct ttacgaaaat cttttctttc
    22201 taacttctta ccggtaattt cacttaacgc atgatagaca cctgtcgact caattaaact
    22261 aacgatagct acaataaaga acactaacgt cgatgtcaca tcaaagctaa atcctgagaa
    22321 tctaaacggc actgggatgc ctaaccaacc ggcatgattg acttgattaa tatcgaccat
    22381 cccaagtaag ccagcaccta tcgttcctaa aacgagtcca attaatatgg caatactctt
    22441 aataaatcca gttgtgaatc tttgtaaaac aagaataatg attaatgtca ttaaacctaa
    22501 caaaatgttc ttaacatctc catagtcctt tgcaccttga cctccagcta agtaattcat
    22561 tgctactggc attaaattga taccaatgat agtaacaaca ctacccgtta ctactggtgg
    22621 gaagaatttt acaagatgtg aaaagaaagg cgcgatgata ataactaata tccctgataa
    22681 aaatagcgaa ccgtaaagta catctattcc tttcgtttga ccaattaaaa tcatgggcgc
    22741 aacagccgtg aatgtacatc caagaacgat tggtaatcct gttcctgtta ctttattggc
    22801 ttgtaaaaat gtggcaaccc cacacataaa tatatctact gtaactaagt aagcgatttg
    22861 ttcaggtgta aacttcaaac ttgtaccaac aatgattgga actaagatgg cacctgcgta
    22921 catagctaaa agatgttgaa cacttaggat taaatttttc attattcttc tcccaccaat
    22981 gtcactttgt ttccttctag tgaagcaacc ttgcagagag aagaaactgt taaacctgct
    23041 tcttctaaac gttgatgccc attttggaaa ctcttttcaa caacaatacc aataccagct
    23101 gtcttagcat tcgcttgctg tgcgatatcg tataatccta atgaagcatc accatttgct
    23161 aaaaagtcat cgatgatgag tacagtatct tcttctgata aaaattcttt tgaaacaatg
    23221 accgtacttg ttttattttt agtaaatgaa tgaatagatg tttcataata accatccgtc
    23281 aaagtgctag gttttgcttt tttcgcaaat aaacatggca catcaaaatg cagtgcagcc
    23341 atgattgcag gtgcgatacc ggaagcttca atggttaaga ttttagtaat ccctttatct
    23401 ttaaattgct cgtaaaaagt gcgaccaact tcattcatta actttgcatc aatttgatga
    23461 tttaaaaatc catcgacttt taaaatcttc tcatcaatga caacgccgtc ttcctttact
    23521 ttttgtccta gtaactccac tcttaaaacc tcctcagatt tgtgtgaaac attttgctct
    23581 taaattggtg ctagatacaa aaaaatcccc aaactaaata atagtttcag ggtttatgag
    23641 tgaacgaaca tgcataacga atttgtcatg caatcaatgt aaaagaagtt tcatcaaata
    23701 aactgtgacc atcatataaa atgatataaa tcacccacca tggttacaat ttaatggctg
    23761 aagctactcc tagtattgtg ttgttactca tagtcatgtc gttcaaggca acatggtaga
    23821 aacttctaaa gccatattct ttagattata tgagtttatg taaattattt aacgataata
    23881 gcaaattttc ggcatttttt caataactgc ttaggtaatc ttttaatagt tttataaatg
    23941 tacagagacg tttaatgcga atgaagactt ataacttaat aatgtaaggg ttttacttta
    24001 taataagaag ggtaaaagat taaataaaca aatttttaaa ggggcgacaa ctatggcaga
    24061 tattactgta gtaaatgaca ctggcgaatt atacaacgta atcaatcaaa agaaatctga
    24121 aggttattta gaatcagagc taacaattat tagtaaaagt aaattacacc taaatgactt
    24181 acccgactct gaaatctccc ttatctcaac aagtggaaca tttagtgata gaatgactaa
    24241 actgttaaca ggagaagatg gtgaacatgc agttttatca cgctacaact tagcgcctga
    24301 tgaattagaa aaatataaac aacttatttt agacgataaa atgttagttg tagcggttcg
    24361 tgacaaatca tcacataaag aagttcaaga acataactca gcatatgaag aaattgatat
    24421 tactcatttt gctgaagcat ctaaaggtcc caaagcttag ttatcacact gaattcaaaa
    24481 tgagattact gtactgtcta atttaagata gtacagtttt ttataccatt taaaagttta
    24541 tgaacaatct tttacagcag gcgattatgc tataatatta tttaaattgg ggaggtgttc
    24601 ttatggaatt taaaaccatt caatctgtca aagatccaca ttacaacgaa gcacttaaac
    24661 tttacgatac taagttaaat ttatctttaa ctgaagataa acgtatcatc aaacgctcat
    24721 tagaaaacaa caaaaccgaa aacgattatg tctttatcgt aggcttagaa aacgatgttg
    24781 ttgtcagtct agcaactgca cattatgaag ccacaaccaa ttcagcattt ttaatttatt
    24841 tattcgcaaa agacctaccc aaccatgatg agttaatgac acaaacatta catcaaattg
    24901 aacatgaact aaacttatta tcaaaccttg ttcattctag aagtattaac tttttaatga
    24961 tggaagtccc taaagaacct gcagatttaa cacaagataa cggacttatt attgaacacc
    25021 gtagacagtt tttatacgaa catcaatttg aaagacaaac tgaaattgat tacattcatc
    25081 caactaatga tggtcacact aaaaaagtgg acttattttt aaagaaaaat attaaactga
    25141 caaaggatat ctacggtaca agtgttaaat caaattatat tttgaagtat gtatttgcca
    25201 atggtatcgc acgtgaaatt atttatgctt tattagaaga aatggattta cgtaaaccat
    25261 tttaaacaaa cttgaaatca ctaacaataa cgtttaaact atgtatgaag caagactgac
    25321 gtttcactgc ccgtagacaa acgtcagtat acataaagta tatgcagaaa ggactttaaa
    25381 gtatgttaac taaagaattt gcacaacgcg tagaactaag tgagaagcaa gtccgtaaaa
    25441 ttgttcaaca tttagaagaa cgtggttacc aactaagcaa gactgaatat cgtggtcgtg
    25501 aagcaactga tttcaaagaa gaagatatcg agcttttcaa agacattgcc gacaaagtaa
    25561 aacaaacaaa tagttatgat ctagcgtttg atgaattaga aaaagaaaaa gacttcctgc
    25621 aagtcattgt caaaaacgat gacaaaaact tacctactaa tcaaaatgtc gctcaactag
    25681 tagaagattt acgcctagaa atccagaaaa tgcgcgaaga acgtcaccta cttggtcaaa
    25741 tgatgaatca agtacatcag caacaacaag aattaaaaga acttcaaaat caacttacat
    25801 ctaaaatcga ttcaaatagc gaatccttaa aagccatcca aacatcacaa gaggctatcc
    25861 aagaagcaca agcatctcaa gctaaagtat tggctgaatc aacaaataaa gttgaaaaga
    25921 atgctgttac tgaagataaa gctgacagta aagattcgaa ggttgctgga gtgaatacat
    25981 caacagacgc taagacggat actaaagcag aaaatgcagg cgatggcact gctactaaag
    26041 tagataaaga ggatcaaatt tctgcaactg aagcaattga aaaagccagc gttgagcagt
    26101 ctaaaaatga aaatgcagct gagacttcaa ataaagaagc tacagtagat gcagacgcgc
    26161 aacatgatgc tgaacaacaa gtagcagaag cccatgcaga agcttctaaa caagctactt
    26221 cgaacgattc attggaagcg aaagcagaaa atgacagcac agcttcacag tcagaaatgt
    26281 cagaaccaaa acctcaagaa gagaaaaaag gtttcttcgc acgtttattc aacctgtaaa
    26341 cttgtgattt gaacacaaat ttaatactca attcatatat agctttgatt taaaaactga
    26401 ttttttataa acctatagaa ctgtaattta ctttcaaatt taatactcaa ttcataaaac
    26461 ctgaaacaag ctgatatact ccattcaata tctactttga agagagcata tcaaactaat
    26521 ttgtttcagg ttttttaatt attcattatg attaatattg aattttgaat aaatttttat
    26581 agtatgttac ttttaaatgg gatggctcct gtgtattcct aaatagattc atcttaataa
    26641 gcccattcaa tttaaatgtt tggctgtgtt agtaaatgac gaatcacaca agggcacact
    26701 catattcaca tttactacaa acgccttttt tctcaaacaa aaatatttgt ttacggtagc
    26761 ttttgaacta ccacctgcac acacgaaccc tcaaaagatg ctaattttga ttcgacagaa
    26821 ttttttacaa cgtgtttgtt cgaagcgtct tttcaaggtt gatcatttat tatcttttta
    26881 ttcaataaat atgaggttag caatttttat caaaccgtca tagccacttc tcgattcaag
    26941 gatgcatttg ttttctcata tcttatctct tatgacagtg cacgatatat atcttttcca
    27001 aatattcatc acttttttcc tgttaaaatc atttctatta ttaacgctgt aatgacaact
    27061 tatttaattt attgcattta atgccctgct tcattttcaa atatcaactt taatcgacta
    27121 ggtcataaca ctattaaccc ttttcacctt tctctactaa attcaaaacg atttgtttac
    27181 aaattccctt atgccaacgg tcgaatttca tcatgtttac atttaattgc aaatgccctc
    27241 ttgaaatatt ttaaaattaa tattattctt agcatactag tcggaattat attcaaaaat
    27301 ttgaggagtg acgttcatga atgcttttct tacactgatt aatattattg tattggtgat
    27361 tttcatcgta atattgcaca tgatggctcg caaacatata tcttttgcga aacgtgtgtt
    27421 tacagcttta ggtatcggca ttgtatttgg cgtgttatta catcttgtct atggaacaca
    27481 ttcgaatgtt attacatcaa caagtgactg gttcaacatt gtcggacaag gttacgttgc
    27541 attgttgcaa atgattgtga tgcctttaat tttcatttct attgtcgctg cattcactaa
    27601 aattcaaatc ggagaaaagt ttgctaaaat tggtagtctc atttttatct ttttaattgg
    27661 cactgtcacc attgctgcaa ttgttggtgt tgtgtatgcc ttggtgtttg gtttagatgc
    27721 atcaacaatt aatcttggta acgctgaaca agcacgtgga agcgaaattg ctaaacaagc
    27781 gaaagattta acagcacata cgttaccaca acaaattttg gaattattgc ctaaaaatcc
    27841 attcttagat ttcactggac aacgtgcaac ttccacgatt gctgtcgtga tatttgcttc
    27901 gtttatagga tttgcttatt tacgcgtagc acgcaaacaa cctgatcatg gtgaattgct
    27961 taagcgtgcg attgacgcta tctattcact agtaatggcg attgtgacgt tcgtcttacg
    28021 tttaacgcca tacggtgtat tagcaattat ggccaacaca ttatcgacaa gtgactttgg
    28081 cgccatttgg actttaggaa aattcttaat cgcatcgtac gctgcattaa ttacgatgta
    28141 tatcattcac ttaattattt taagcctgtt aggtattagc ccaattcgct atgttaaaaa
    28201 gactttggaa gtattaatct ttgcattcac atcacgctca agcgcgggtg ccttaccatt
    28261 gaatgtacaa acacaaacaa gacgtctagg cgtgccagaa ggtattgcca atttcgctgc
    28321 gacatttgga ttgtcgattg gacaaaatgg ttgtgcaggc atttaccctg ccatgcttgc
    28381 aattatggtc gcacccgtcg ctaatgtaga aattgattta caatttattg taacgttaat
    28441 cgctgtcgtg attattagtt cattcggcgt tgctggcgtc ggtggcgggg ctacatttgc
    28501 atccatattg gtgttgtcaa cgttaaactt gccagtcgct ttagccggcg tgctcatctc
    28561 cgttgaacca ttgattgaca tgggtagaac agcattaaac gtgaacgatt caatgctagc
    28621 tggtacaggt accgccaagt taacaaaaca ttgggacaaa gatactttcg aatcaaatga
    28681 taatgctgca ttaacttcac actaattcat tgcaagaaat acccgccccc tctagctata
    28741 ctt
//
LOCUS       DAOBBH010000013        28259 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.013, whole genome shotgun sequence.
ACCESSION   DAOBBH010000013 DAOBBH010000000
VERSION     DAOBBH010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 28259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28259
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.013"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(300..1298)
                     /gene="sirC"
                     /locus_tag="P6L01_000389"
     CDS             complement(300..1298)
                     /gene="sirC"
                     /locus_tag="P6L01_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirC"
                     /protein_id="HDP3029485.1"
                     /translation="MTEKINKKDNYHLIFALIFLAIVSVVSMMIGSSFIPLQRVLMYF
                     INPNDSMDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGAS
                     LSAVVFIAFFSHLTIHLLPLFAVLGGAVAMMILLVFQTKGQIRPTILIIIGISMQTLF
                     IALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKI
                     SILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGS
                     YAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL"
     gene            complement(1295..2290)
                     /gene="sirB"
                     /locus_tag="P6L01_000390"
     CDS             complement(1295..2290)
                     /gene="sirB"
                     /locus_tag="P6L01_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirB"
                     /protein_id="HDP3029486.1"
                     /translation="MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFD
                     SENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFV
                     IFSITFIQIQSFKMIVVIAFLGAIVVTILVVALGMFRQTLFSPHRVILAGAAIAMLFT
                     AFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILM
                     TSDDIATGLGQNIKLIKWMIIMLISMLAGISVAVAGSIVFVGLIVPNISKRLLPPNYK
                     YLIPFTALAGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYIMKKGRQRL"
     gene            complement(2306..3298)
                     /gene="sirA"
                     /locus_tag="P6L01_000391"
     CDS             complement(2306..3298)
                     /gene="sirA"
                     /locus_tag="P6L01_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter
                     substrate-binding protein SirA"
                     /protein_id="HDP3029487.1"
                     /translation="MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAM
                     GTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQ
                     EPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
                     KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILN
                     DLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESE
                     WTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK"
     gene            3529..4509
                     /gene="sbnA"
                     /locus_tag="P6L01_000392"
     CDS             3529..4509
                     /gene="sbnA"
                     /locus_tag="P6L01_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HDP3029488.1"
                     /translation="MIEKSQACHDSLLDSVGQTPMVQLHQLFPKHEVFAKLEYMNPGG
                     SMKDRPAKYIIEHGIKHGLITENTHLIESTSGNLGIALAMIAKIKGLKLTCVVDPKIS
                     PTNLKIIKSYGANVEMVEEPDAHGGYLMTRIAKVQELLATIDDAYWINQYANELNWQS
                     HYHGAGTEIVETIKQPIDYFVAPVSTTGSIMGMSRKIKEVHPNAQIVAVDAKGSVIFG
                     DKPINRELPGIGASRVPEILNRSEINQVIHVDDYQSALGCRKLIDYEGIFAGGSTGSI
                     IAAIEQLITSIEEGATIVTILPDRGDRYLDLVYSDTWLEKMKSRQGVKSE"
     gene            4506..5516
                     /gene="sbnB"
                     /locus_tag="P6L01_000393"
     CDS             4506..5516
                     /gene="sbnB"
                     /locus_tag="P6L01_000393"
                     /EC_number="1.5.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate
                     dehydrogenase SbnB"
                     /protein_id="HDP3029489.1"
                     /translation="MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPL
                     KPYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRNMERASGVIILN
                     DPETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLE
                     QFDHIKRVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQ
                     PYIEYDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFS
                     KEALHAELGQLVTGDIPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLN
                     LY"
     gene            5537..7291
                     /gene="sbnC"
                     /locus_tag="P6L01_000394"
     CDS             5537..7291
                     /gene="sbnC"
                     /locus_tag="P6L01_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis protein SbnC"
                     /protein_id="HDP3029490.1"
                     /translation="MQNHTAVNTAQAIILRDLVDALLFEDIAGIVSNSEITKENGQTL
                     LIYERETQQIKIPVYFSALNMFRYESSQPITIEGRVSKQPLTAAEFWQTIANMNCDLS
                     HEWEVARVEEGLTTAATQLAKQLSELDLASHPFVMSEQFASLKDRPFHPLAKEKRGLR
                     EADYQVYQAELNQSFPLMVAAVKKTHMIHGDTANIDELENLTVPIKEQATDMLNDQGL
                     SIDDYVLFPVHPWQYQHILPNVFATEISEKLVVLLPLKFGDYLSSSSMRSLIDIGAPY
                     NHVKVPFAMQSLGALRLTPTRYMKNGEQAEQLLRQLIEKDEALAKYVMVCDETAWWSY
                     MGQDNDIFKDQLGHLTVQLRKYPEVLAKNDTQQLVSMAALAANDRTLYQMICGKDNIS
                     KNDVMTLFEDIAQVFLKVTLSFMQYGALPELHGQNILLSFEDGRVQKCVLRDHDTVRI
                     YKPWLTAHQLSLPKYVVREDTPNTLINEDLETFFAYFQTLAVSVNLYAIIDAIQDLFG
                     VSEHELMSLLKQILKNEVATISWVTTDQLAVRHILFDKQTWPFKQILLPLLYQRDSGG
                     GSMPSGLTTVPNPMVTYD"
     gene            7284..8540
                     /gene="sbnD"
                     /locus_tag="P6L01_000395"
     CDS             7284..8540
                     /gene="sbnD"
                     /locus_tag="P6L01_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B export MFS transporter"
                     /protein_id="HDP3029491.1"
                     /translation="MINQSIWRSNFRILWLSQFIAIAGLTVLVPLLPIYMASLQNLSV
                     VEIQLWSGIAIAAPAVTTMIASPIWGKLGDKISRKWMVLRALLGLAVCLFLMALCTTP
                     LQFVLVRLLQGLFGGVVDASSAFASAEAPAEDRGKVLGRLQSSVSAGSLVGPLIGGVT
                     ASILGFSALLMSIAVITFIVCIFGALKLIETTHMPKSQTPNINKGIRRSFQCLLCTQQ
                     TCRFIIVGVLANFAMYGMLTALSPLASSVNHTAIDDRSVIGFLQSAFWTASILSAPLW
                     GRFNDKSYVKSVYIFATIACGCSAILQGLATNIEFLMAARILQGLTYSALIQSVMFVV
                     VNACHQQLKGTFVGTTNSMLVVGQIIGSLSGAAITSYTTPATTFIVMGVVFAVSSLFL
                     ICSTITNQINDHTLMKLWELKQKSAK"
     gene            8530..10266
                     /gene="sbnE"
                     /locus_tag="P6L01_000396"
     CDS             8530..10266
                     /gene="sbnE"
                     /locus_tag="P6L01_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,3-diaminopropanoate--citrate ligase SbnE"
                     /protein_id="HDP3029492.1"
                     /translation="MQNKELIQHAAYAAIERILNEYFREENLYQVPPQNHQWSIQLSE
                     LETLTGEFRYWSAMGHHMYHPEVWLIDGKSKKITTYKEAIARILQHMAQSADNQTAVQ
                     QHMAQIMSDIDNSIHRTARYLQSNTIDYVEDRYIVSEQSLYLGHPFHPTPKSASGFSE
                     ADLEKYAPECHTSFQLHYLAVHQDVLLTRYVEGKEDQVEKVLYQLADIDISEIPKDFI
                     LLPTHPYQINVLRQHPQYMQYSEQGLIKDLGVSGDLVYPTSSVRTVFSKALNIYLKLP
                     IHVKITNFIRTNDLEQIERTIDAAQVIASVKDEVETPHFKLMFEEGYRALLPNPLGQT
                     VEPEMDLLTNSAMIVREGIPNYHADKDIHVLASLFETMPDSPISKLAQVIEQSGLAPE
                     AWLECYLDRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPDVCFVRDLEGICLSRTI
                     ATEKQLVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWQLV
                     AHRLMTWKKEYANNAVFVDCVEDLYQTPTIAAKANLMSKLNDCGANPIYTHIPNPICY
                     NKEVSYCESNNS"
     gene            10175..12025
                     /gene="sbnF"
                     /locus_tag="P6L01_000397"
     CDS             10175..12025
                     /gene="sbnF"
                     /locus_tag="P6L01_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(L-alanin-3-ylcarbamoyl)-2-[(2-
                     aminoethylcarbamoyl)methyl]-2-hydroxypropanoate synthase
                     SbnF"
                     /protein_id="HDP3029493.1"
                     /translation="MIVVQTLFIHIYQIQFVITRRYRIVNQTILNRVKTRVMHQLVSS
                     LIYENIVVYKASYQDGVGHFTIEGHDSEYRFTAEKTHSFDRIRITSPIERVVGDEADT
                     TTDYTQLLREAVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFNDYE
                     FYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKVETTVSRNVVVN
                     EMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHPWQFEHVIQVDLAEERLNGTVLW
                     LGESDELYHPQQSIRTMSPIDTTKYYLKVPISITNTSTKRVLAPHTIENAAQITDWLK
                     QIQQQDMYLKDELKTVFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLIDE
                     EDAIPFNALYASDKDGLPFIEKWIKQYGSEAWTKQFLAVAIRPMIHMLYYHGIAFESH
                     AQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIE
                     TDDERLVRDFLHDAFFFINIAEIILFIEKQYGIDEQRQWQWVKDIIEAYQEAFPELNN
                     YQHFDLFEPTIQVEKLTTRRLLSDSELRIHHVTNPLGVGGINDATTISET"
     gene            12000..12776
                     /gene="sbnG"
                     /locus_tag="P6L01_000398"
     CDS             12000..12776
                     /gene="sbnG"
                     /locus_tag="P6L01_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis citrate synthase
                     SbnG"
                     /protein_id="HDP3029494.1"
                     /translation="MQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDT
                     EHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDRETV
                     EHIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDD
                     IAQVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEVVNAHGKHFCALPREDE
                     DIAKWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDDG"
     gene            12776..13978
                     /gene="sbnH"
                     /locus_tag="P6L01_000399"
     CDS             12776..13978
                     /gene="sbnH"
                     /locus_tag="P6L01_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis decarboxylase
                     SbnH"
                     /protein_id="HDP3029495.1"
                     /translation="MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITSSLPSNCQ
                     MYYAMKANSERTILDTISQYVEGFEVASQGEIAKGLAFKPANHIIFGGPGKTDEELRY
                     AVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFG
                     ISEDEVDDVIEAALVMPNIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKSWSEKHRF
                     PLKHINLGGGIGVNYADLTSQFEWDNFVENFKTLIVEQEMEDVTLNFECGRFIVAHIG
                     YYVTEVLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFEIYRYKDNPYSFEKVSISR
                     QDTTLVGQLCTPKDVFAREVQIDAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIY
                     LTQTKEDE"
     gene            13982..14746
                     /gene="sbnI"
                     /locus_tag="P6L01_000400"
     CDS             13982..14746
                     /gene="sbnI"
                     /locus_tag="P6L01_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional
                     regulator/O-phospho-L-serine synthase SbnI"
                     /protein_id="HDP3029496.1"
                     /translation="MNHIHEHLKLVPVDKIDLHETFEPLRLEKTKSSIEADDFIRHPI
                     LVTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETL
                     QQEHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVER
                     IAQGTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLAL
                     LKQDDDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ"
     gene            14942..15568
                     /locus_tag="P6L01_000401"
     CDS             14942..15568
                     /locus_tag="P6L01_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDP3029497.1"
                     /translation="MEKNVEKSFIKIGLYFQIAYIVLMAITLCGFVICYGLIFGLFYL
                     LSGSRADYLIVTIVISAIISIFVIILSIVPVIVLASDLFKERISKGVILIVLAIIALV
                     LCNFVSAILWFVSAISILGRKKLVAAADTTTIQKSKGNANQASHKDTCKKELDSQDMM
                     EHPEVKNPTTKNLEGFNEEIHKDEATTKVVSDNTEPPIESKGHVSKKD"
     gene            15780..16556
                     /locus_tag="P6L01_000402"
     CDS             15780..16556
                     /locus_tag="P6L01_000402"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HDP3029498.1"
                     /translation="MTNNKVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAA
                     ALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITE
                     EQFKTVYGVNVAGVLWGIQAAHEQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFA
                     VRGLTQVAAQDLASEGITVNAFAPGIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQI
                     ALGRVSQPEDVSNVVSFLAGKDSDYITGQTIIVDGGMRFR"
     gene            16700..16822
                     /locus_tag="P6L01_000403"
     CDS             16700..16822
                     /locus_tag="P6L01_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029499.1"
                     /translation="MHQLKALLVLTHPRYYKTSQKHHYLIYLNKNSQSYLILFL"
     gene            16892..17872
                     /locus_tag="P6L01_000404"
     CDS             16892..17872
                     /locus_tag="P6L01_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDP3029500.1"
                     /translation="MEDLERVLITGGAGFIGSHLVDDLQQDYDVYVLDNYRTGKRENI
                     KSLADDHVFELDIREYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVAT
                     LRLLEIIKKYNSHIKRFIFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTL
                     NYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDF
                     VYVYDVVQSVRLIMEHKDAIGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHEFKEARK
                     GDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMS"
     gene            17835..18527
                     /locus_tag="P6L01_000405"
     CDS             17835..18527
                     /locus_tag="P6L01_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDP3029501.1"
                     /translation="MKKLQLKKWKCRENDIEAVHNNKGYAYQRKLDALEEVRKGYYPI
                     KRAIDLVLSIVLLFLTFPIMFIFAIAIVIDSPGNPIYSQVRVGKMGKLIKIYKLRSMC
                     KNAEKNGAQWADKDDDRITNVGKFIRKTRIDELPQLINVVKGEMSFIGPRPERPEFVE
                     LFSSEVIGFEQRCLVTPGLTGLAQIQGGYDLTPQQKLKYDMKYIHKGSLMMELYISIR
                     TLMVVITGEGSR"
     gene            18737..19903
                     /locus_tag="P6L01_000406"
     CDS             18737..19903
                     /locus_tag="P6L01_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDP3029502.1"
                     /translation="MKIIYCITKADNGGAQTHLIQLANHFCVHHDVYVIVGNHGPMIE
                     QLDARVNVIILEHLVGPIDFKQDILAVKVLAQLFSKIKPDVIHLHSSKAGTVGRIAKF
                     ISKSKDTRVVFTAHGWAFTEGVKPAKKFLYLVIEKLMSCITDSIICVSDFDKQLALKY
                     RFNRLKLTTIHNGIADVPAVKQTLKSQSHNNIGEVVGMLPNKQDLQINAPTKHQFVMI
                     ARFAYPKLPQNLIAAIEILKLHNSNHAHFTFIGDGPTLNDCQQQVVQAGLENDVTFLG
                     NVINASHLLSQYDTFILISKHEGLPISIIEAMATGLPVIASHVGGISELVADNGICMM
                     NNQPETIAKVLEKYLIDSDYIKMSNQSRKRYLECFTEEKMIKEVEDVYNGKSTQ"
     gene            19884..21122
                     /locus_tag="P6L01_000407"
     CDS             19884..21122
                     /locus_tag="P6L01_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDP3029503.1"
                     /translation="MENQHNSKLLTLLLIGLAVFIQQSSVIAGVNVSIADFITLLILV
                     YLLFFANHLLKANHFLQFFIILYTYRMIITLCLLFFDDLIFITVKEVLASTVKYAFVV
                     IYFYLGMIIFKLGNSKKVIVTSYIISSVTIGLFCIIAGLNKSPLLMKLLYFDEIRSKG
                     LMNDPNYFAMTQIITLVLAYKYIHNYIFKVLACGILLWSLTTTGSKTAFIILIVLAIY
                     FFIKKLFSRNAVSVVSMLVIMLILLCFTFYNINYYLFQLSDLDAVPSLDRMASIFEEG
                     FASLNDSGSERSVVWINAILVIKYTLGFGVGLVDYVHIGSQINGILLVAHNTYLQIFA
                     EWGILFGALFIIFMLYLLFELFRFNISGKNVTAIVVMLTMLIYFLTVSFNNSRYVAFI
                     LGIIVFIVQYEKMERDRNEE"
     gene            21112..22542
                     /locus_tag="P6L01_000408"
     CDS             21112..22542
                     /locus_tag="P6L01_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDP3029504.1"
                     /translation="MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVS
                     FSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLFLALTVFAMYM
                     VVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVI
                     FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPN
                     GQLNLYTSISCVVLGLVGTYQQVGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQQ
                     SHSLTKTLANNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFASTVPLMTILAILVLI
                     IPLNMLISRQYLLIVNKIRLYNASITIGAVMNLVLCLVLIYFYGIYGAAIARLITEFI
                     LLIWRFVDITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIV
                     VYLLLMMTMKNQYVWQILRHLRHKTI"
     gene            22810..23409
                     /locus_tag="P6L01_000409"
     CDS             22810..23409
                     /locus_tag="P6L01_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDP3029505.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
     gene            23776..24501
                     /gene="sasD"
                     /locus_tag="P6L01_000410"
     CDS             23776..24501
                     /gene="sasD"
                     /locus_tag="P6L01_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasD"
                     /protein_id="HDP3029506.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVPNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKTLPQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPLTKDGNFYQTNVDANGVNHGGSEMVQNKTGHMSQQDHMN
                     QNTHMNQQPQIQQGHMQSSNHQMMSPKANMHSSNHQMNQSNKKVLPAAGESMTSSILT
                     ASIAALLLVSGLFLAFRRRSTNK"
     gene            complement(24692..25447)
                     /locus_tag="P6L01_000411"
     CDS             complement(24692..25447)
                     /locus_tag="P6L01_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDP3029507.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            25683..26390
                     /gene="deoD"
                     /locus_tag="P6L01_000412"
     CDS             25683..26390
                     /gene="deoD"
                     /locus_tag="P6L01_000412"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDP3029508.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            26397..27749
                     /gene="tet(38)"
                     /locus_tag="P6L01_000413"
     CDS             26397..27749
                     /gene="tet(38)"
                     /locus_tag="P6L01_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HDP3029509.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVILNRWTQSEK
                     "
     gene            27830..>28259
                     /gene="deoC"
                     /locus_tag="P6L01_000414"
     CDS             27830..>28259
                     /gene="deoC"
                     /locus_tag="P6L01_000414"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDP3029510.1"
                     /translation="MKFEKYIDHTLLKPESTRTQIDQIIDEAKAYNFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVVKAAKGHTVKVIIETVLLDHDEIVKASELTK"
BASE COUNT         9486 a         4362 c         5393 g         9018 t
ORIGIN      
        1 ttacaattaa aaataatatg atatttttat taaaatttgt gtttttgaat aaatggcatt
       61 atcaaacact atatttaaat aagaaatagt tatcgatatt tgaaaaatcg aagaatattg
      121 attgatatta tagaatatga gtcggtgtta tttgtatata aagtattaat atgtaattaa
      181 gaaagtcgac gcttgttaac aataacaaca actatatgta taaaaaaacc tcgcaactgt
      241 tagttaactg aatcatttag ctaacttcgt tgtgaggtca ttttgtttta ataatatcgt
      301 tataactttt tcacggttaa taataagtat atgaagaatg gggcaccaaa agcagcaata
      361 aatacacctg ctggcacttc tttaggcaag aataaggtac gcccaattaa gtctgcaata
      421 acaattgata tggcaccaat cattgctgac attagtaact ttttagcata acttccgcga
      481 acgattgttt tcgcgatatg tggtgcgatt aaaccgacaa acccaatgtt acctactaaa
      541 ctgattgcca tagatacgag tatagtagaa gtgattaatt ggattagttt catacgttgt
      601 acatgtaagc ctaagccaat cgctacaggg tcatcaagta tagatatttt catttttggt
      661 ataacaagaa ataacaacgg cacaacagct aaaataacca tacccaaaat gattgtatct
      721 ttaaacgtag caccgtaaag acttccgact agccatgtat aagctttggc agcagataat
      781 tgcttcgttg taatgagtaa tccttggaca agcgcaataa acaacgtttg catcgaaata
      841 ccgatgatta tgagtattgt cgggcgtatt tgtcctttcg tttgaaacac taatagtatc
      901 atcattgcaa ctgcgccacc taatactgca aatagtggaa gtaaatgtat cgttaaatgg
      961 ctgaaaaatg caataaagac aacagcactt aagctagcac cacctgtgat accgataata
     1021 tcaggtgagg caattggatt ttttaataca ttttgcaaca ttaaaccact cattcctagt
     1081 gcggcacctg ctaaaatcgc aagtgtaatg cgaggtaagc gtaatacttc taaagtgaat
     1141 tgatccatac tgtcatttgg atttataaag tacatcagta cgcgttgtaa tggtataaag
     1201 cttgaaccaa tcatcatact taccactgaa acgatggcta aaaagattaa cgcgaagatg
     1261 agatggtaat tgtctttttt attaatcttt tcggtcataa gcgttgacgt cctttcttca
     1321 taatatagat taagacaata gcgccaatga cagcggtaac gacaccgata ggcaactcta
     1381 gtggcttaat tattatacga gcaacaatgt ctgaaatgat cattaggatt gctccagcta
     1441 atgcagtaaa aggaattaaa tacttatagt ttggtggtaa taatcgtttg ctaatattcg
     1501 gtacgataag acccacaaag acgattgatc cagctacggc taccgaaata ccggctaaca
     1561 tactgatgag cataataatc atccatttga ttaattttat gttttgaccg aggccggttg
     1621 caatgtcgtc acttgtcatc aagatgttga tgtgtgcagc catgctaaat gcaattaaaa
     1681 taagtatcaa tacaagcgga ataatccatg ggatatccca aatattacgt aatgaaacgg
     1741 agccacttaa ccaaaataat aggccttgta agtctgtttc gttcataata agtatgcctt
     1801 gagtaaaggc tgtaaatagc atcgcaatcg cagcacctgc caaaatgaca cggtgaggtg
     1861 agaatagtgt ttgtctaaac atacctagcg caacaactaa tatagtaaca acaatggcac
     1921 ctaaaaatgc aataactaca atcattttaa aagattgaat ttggataaat gtaatactaa
     1981 aaatgacaaa aaatactgcg cctgcattga caccgaaaag ccctggtgag gctattgggt
     2041 ttcgtgtaag tgcttgcatc aacaaacctg agacagcaag ggcagcacca gtcaataacg
     2101 caatgattgt tctcgacgcc cgtgcaccag tgacaacatc atgtaaatcg ttttcactat
     2161 caaagttgaa taacgcctgt atcaccgtac ctggtgacac aagcgtattt ccaatcatta
     2221 aacttaagat agctactatt gcaagacata aaccagcaat aacgatttgg tattttggtt
     2281 taagtagcat cgtaaaactc cttaattatt ttgattgttt ttcaatattt aacttttcat
     2341 ataaatcgtc aataagtttt aatgaagatt tatatccgcc agctaagttc caagtgattt
     2401 catctaaatc atcagatact tggttgtttt taactgcgtc taaatttttc cactctttac
     2461 ttgaagtcca ttcgctttca gtctttttaa ctaatgcagc atctttcgca tttggatctg
     2521 attttactac aaaaatatga tcagcgttca ttaatggaat gctttcttta gatgtaagtt
     2581 ggataatatc tttaccatta tcaacttgtt tttgtaagtc tttattacgt ttgaatccta
     2641 aatcatttaa gatttcacca gcatatccac cagcataaat tcttgtatga tcagcacgga
     2701 agttaacaac tgaagctttc aatggccatg catctttata ctttgctttt gcatcttttt
     2761 ggaatgcagc tactttatca tcgtactttt taagtaaatc ttcagcttct ttttctttcc
     2821 ctaaagcttt ccccattaac ttagttgtat ctttgaattt gaaaactgta tcagtagaaa
     2881 ctgttggtgc gattttagat aattgatcgt aaactttttc atttctaact tttgacgcga
     2941 caattaagtc cggttttaat ttagagattt cctctaagtt aggtgcaggt tcttgaccta
     3001 caatcttagt atcttttaaa tcatttttta tgtattcgaa tttcggtttt tgtgtccatg
     3061 attctacagc acctacaggt ttaacaccta aagatacagc gacgtcagtg gcaccttgat
     3121 atagcgtaac aacacgcttt ggtttccctt taatttcagt tgtacccatt gcatgtttaa
     3181 ttgaagttgt ttccttatct ttgttatcag atgattgttt atttgaattc ccactacatc
     3241 ctgctaaaac aagtaggaaa gcaagcgtaa caacaagcat tttaattact ttattcattg
     3301 actaattagc ctccttcgtg atgtatgaca atgagaatca ttatcacgat ttagtatgaa
     3361 ttaaattttt tcctaagtca ataaaatatt tatgatttac atgcaactta taattatttg
     3421 acatataaat gcataaaaaa tataatccta attacttgat agtgagaatc attatcaatt
     3481 aggtaacgca caatattata gaattttaaa tttgaggagg aagcgctttt gattgaaaaa
     3541 agtcaagcat gtcacgattc attgttagat tctgtagggc aaacacctat ggttcaactt
     3601 catcaactat ttccgaaaca tgaagtgttt gcaaagttag agtatatgaa tcctggaggc
     3661 agcatgaaag atcgacctgc caagtacatc attgaacatg gtattaaaca tggtttaatc
     3721 actgagaata cacatttaat tgaaagtact tctggtaatt taggcattgc gttggcaatg
     3781 atagctaaaa tcaagggatt aaaactcacg tgtgttgttg atcctaaaat atcaccaaca
     3841 aatttgaaaa ttattaaaag ttatggtgcc aatgtagaaa tggttgaaga acctgatgca
     3901 catgggggtt atttaatgac tcgtattgca aaggtgcaag aactgttagc cactattgac
     3961 gatgcatatt ggattaatca atatgcgaat gagttaaatt ggcaatccca ttatcatggt
     4021 gcaggcacag agattgttga aacaattaag caacctatag attattttgt cgcgccagtc
     4081 agcacgacag gtagcattat gggtatgagt agaaaaataa aagaagtgca tccaaacgca
     4141 caaattgttg ctgttgatgc gaaagggtca gtcatttttg gtgacaaacc tattaataga
     4201 gaattacctg gtatcggtgc tagtcgtgta cccgaaatat tgaatagatc agaaattaat
     4261 caagtgatcc atgtagatga ttatcaatct gctttgggct gtcgaaaact gattgattat
     4321 gaaggcatat ttgccggagg ttcaacaggc tcgattattg cagcgattga gcagttgata
     4381 acgtcaattg aagaaggtgc aacaattgtc acgattttac cagatcgagg cgatcgttac
     4441 ttagatttag tttattcaga tacatggtta gaaaaaatga aatcaagaca aggagttaaa
     4501 tcagaatgaa tagagagatg ttgtatttaa atagatcaga tattgaacaa gcgggaggta
     4561 atcattcaca agtttatgtg gacgcattaa cagaagcatt aacagcccat gcgcacaatg
     4621 attttgtaca accgcttaag ccgtatttaa gacaggatcc tgaaaatgga cacatcgcag
     4681 atcgaattat tgcaatgcca agtcatatcg gtggtgaaca cgcaatttca ggtattaagt
     4741 ggataggtag taagcacgac aatccatcga aacgtaatat ggagcgtgca agtggtgtca
     4801 ttattttgaa tgatccagaa acgaattatc caattgcagt tatggaagca agtttaatta
     4861 gtagtatgcg tactgcagca gtttcagtga ttgcagctaa gcatttggct aaaaaaggat
     4921 ttaaagactt aacaatcatt ggttgcgggc taatcggaga caagcaatta caaagtatgt
     4981 tagagcaatt cgatcatatt aaacgcgtgt ttgtttacga tcaattctct gaagcatgtg
     5041 cacgctttgt tgatagatgg caacaacagc gtccggaaat taattttatt gcgacagaaa
     5101 atgctaaaga agcagtatca aatggtgaag tagtcattac atgtaccgta acggatcaac
     5161 catacattga atatgattgg ttacaaaagg gtgcatttat tagcaacatt tctatcatgg
     5221 atgtgcataa agaagtcttt attaaagctg acaaagtcgt agtagatgac tggtcacaat
     5281 gtaatcgaga aaagaaaact attaaccaat tggtgttaga aggtaaattc agcaaagaag
     5341 cacttcatgc tgaactaggg caacttgtga caggtgacat accaggacgt gaagacgatg
     5401 atgaaatcat attacttaat ccgatgggta tggctatcga agatatttca agtgcttatt
     5461 ttatttatca acaggcacaa caacaaaata ttgggacaac attgaaccta tattaagaat
     5521 gcgaggtgtc tgaacattgc agaatcatac agcagtcaat acagcacaag cgataatatt
     5581 aagagattta gttgatgcat tattatttga agatatagcc ggaattgtat cgaatagtga
     5641 gattactaaa gaaaacggac aaacgctttt gatatacgaa cgtgaaacac aacaaataaa
     5701 gatacctgtt tattttagtg ctttaaatat gtttcgttac gaaagttcac aaccaattac
     5761 gatagaggga agggtgtcta agcaaccttt aacggcagct gaattttggc aaacaattgc
     5821 taatatgaat tgtgatctaa gtcatgaatg ggaagtggct cgcgttgaag aaggactgac
     5881 tactgctgcc acacagcttg ctaaacaatt atcagaatta gatttagcgt cacatccttt
     5941 tgtgatgtca gagcagtttg caagtttaaa agatcgtcca tttcatccat tagctaaaga
     6001 aaaaagagga ttaagagaag cggattatca agtgtatcaa gctgaattaa atcaatcatt
     6061 tcctttaatg gttgcagcag ttaaaaagac acatatgatt catggcgata ctgcaaatat
     6121 cgatgaatta gaaaatttga cagtacctat aaaagaacaa gcgacagaca tgttaaatga
     6181 tcaagggtta tcaatagatg actatgtact atttccggta catccttggc aatatcagca
     6241 tattctgccg aacgtctttg cgacagagat tagtgaaaag ttggttgtac tattaccgtt
     6301 aaaatttgga gattatctgt cgtcttcaag tatgcgttca ttaattgata ttggcgcacc
     6361 gtataaccat gtcaaagtac catttgcaat gcagtcatta ggcgcattaa ggctaacgcc
     6421 tacgcgttac atgaaaaacg gagaacaagc agaacaatta ttacgtcagc ttatagaaaa
     6481 agatgaagca ctagctaagt atgtcatggt ttgtgatgaa acagcttggt ggtcatatat
     6541 gggtcaagat aatgatattt tcaaagatca attaggtcat ctaactgttc agctaagaaa
     6601 gtatcccgaa gtgctagcca aaaatgatac gcaacagcta gtgtcaatgg cagcactcgc
     6661 ggcaaatgat cgcactttat atcaaatgat ttgtggaaaa gataatattt ctaaaaatga
     6721 tgtcatgacg ttatttgaag atatcgcgca agtcttttta aaggtaacac tatcatttat
     6781 gcaatacggc gcattaccag agttgcatgg tcaaaatata ttgttgtcat ttgaagatgg
     6841 acgtgtacaa aaatgcgtgt tacgtgatca tgatactgtc agaatttata aaccatggct
     6901 aacagcacat cagctttcat tgccgaagta tgtcgtcaga gaagatacac ctaatacgct
     6961 aattaatgag gatttggaaa cattctttgc ttattttcaa acattagctg tatcggtaaa
     7021 tctatatgcc attattgatg caattcaaga tttatttggt gtaagtgagc atgaacttat
     7081 gtcgttgtta aaacaaattt taaaaaatga agtggcaact atttcctggg ttacaactga
     7141 tcagctagct gtcagacaca ttttatttga taaacagacg tggccattca aacaaatttt
     7201 attaccattg ctatatcaac gtgatagtgg tggaggtagt atgccttcag gtttaactac
     7261 cgtaccaaat ccaatggtga catatgatta atcagtctat atggcgcagt aactttcgca
     7321 ttttatggct cagtcagttt atagcgattg ctggactgac agtacttgtg ccattattgc
     7381 caatttatat ggcatcacta caaaatctat cagtcgtaga aatacagttg tggagtggta
     7441 tagcgattgc tgctccagct gtaacgacga tgatagcttc gccgatatgg gggaagctag
     7501 gtgataagat cagccgaaaa tggatggtct taagagcgtt acttggtttg gcggtatgct
     7561 tatttttaat ggcattgtgt acgacaccat tacagtttgt acttgtgagg ttattgcagg
     7621 gactatttgg tggtgttgtt gatgcatcaa gtgcgtttgc gagtgcagag gcgccagctg
     7681 aagatcgtgg aaaggtatta ggaagactgc aaagttcagt cagcgcaggg tctcttgtgg
     7741 ggccattaat tggcggtgtt acagcttcga tattaggttt tagtgcgtta ctgatgagta
     7801 ttgccgttat tacttttatt gtctgtattt tcggtgcatt aaaattgatt gaaacgacac
     7861 atatgccaaa atcacaaaca ccaaatatta ataaaggtat tcgccgttca tttcaatgtc
     7921 tattatgcac acaacaaaca tgtcgattta ttatcgttgg cgttttagca aactttgcta
     7981 tgtatggcat gctaactgca ttatcaccac ttgcttcatc agtgaatcat acagcgatag
     8041 atgaccgtag tgtgattgga tttttacagt ccgcattttg gacggcttcg atattaagcg
     8101 cgcctttatg gggacgcttt aatgataaat catatgttaa atcagtatat atatttgcca
     8161 cgattgcatg tggttgtagt gcgatactgc aaggtttagc gacgaatata gagtttttaa
     8221 tggctgcaag aatacttcaa ggattaacat atagtgcatt gattcaaagt gtcatgtttg
     8281 ttgtcgtgaa tgcgtgtcat caacaactta aaggcacatt tgttggaacg acgaacagta
     8341 tgttagttgt tggtcaaatt attggcagtc ttagtggcgc tgccattaca agttatacta
     8401 caccagctac tacgtttatc gttatgggcg tagtatttgc agtaagtagt ttatttttaa
     8461 tttgttcaac catcactaat caaatcaacg atcacacatt aatgaaatta tgggagttga
     8521 aacaaaaaag tgcaaaataa agaattaata caacatgcag cgtatgcggc tatcgaacgc
     8581 attttaaatg aatattttag agaagaaaat ttatatcaag taccacctca aaatcatcaa
     8641 tggtctatac aattatcaga gctcgaaact ttaacgggtg aatttcgcta ttggtctgcg
     8701 atggggcatc atatgtatca tccagaggta tggcttatcg atggaaaaag taaaaaaata
     8761 acaacttata aagaagcaat tgcgcgtatt ttgcaacata tggctcaaag tgcagataat
     8821 caaacggcag tgcaacaaca tatggcgcaa attatgtctg acatcgataa tagcattcat
     8881 cgcacggcgc gttatttgca aagtaacaca atagactacg tagaggatcg ttatatcgtt
     8941 tcagaacaat ctttatactt aggtcatcca tttcatccga ctcctaagag tgcaagtggg
     9001 ttttcagaag cagatttaga gaaatatgca cccgaatgtc atacatcatt ccaattgcat
     9061 tatttagctg tgcatcaaga tgttctgctc acgcgctatg tagaaggtaa agaagatcag
     9121 gttgagaaag tgttgtatca attagcagac atagatatat cagagatacc caaagatttt
     9181 attttattac caacacatcc ttatcaaatc aatgtgttgc gacagcatcc acagtatatg
     9241 caatatagtg aacaaggttt aataaaagac cttggcgttt ccggtgattt agtgtacccg
     9301 acgtcttcgg ttagaactgt attttcaaaa gcattaaaca tttatttaaa attaccgata
     9361 cacgttaaaa ttactaattt tatacgtacg aatgatcttg aacaaattga acggacaatt
     9421 gatgccgcgc aagttatcgc atcagtcaaa gatgaggttg aaacacccca ttttaaattg
     9481 atgtttgaag aaggatatcg tgcattgtta ccgaatccat tagggcaaac agttgaacct
     9541 gaaatggatt tattaacaaa tagtgccatg attgttcgtg aagggatacc gaattaccat
     9601 gctgataaag atattcatgt attggcgtca ttatttgaaa cgatgcctga ttcaccgatc
     9661 tctaagttag cacaagtgat tgagcaaagt ggtttagcac cagaagcatg gcttgaatgt
     9721 tatttggatc gtacattatt gccgatatta aagttgttta gtaacacagg cattagtcta
     9781 gaagcacatg tacaaaatac attaattgaa ttaaaagatg gcatacccga cgtatgcttt
     9841 gtcagagatc ttgaaggcat ttgtctatct agaacgattg ctactgaaaa acagcttgtg
     9901 ccaaatgttg tggcagcatc aagccctgtt gtatatgcac atgatgaagc atggcatcgt
     9961 cttaaatatt atgttgtagt aaatcactta ggacatttag tatcaactat tggtaaagca
    10021 actagaaatg aagttgtgtt atggcaactt gtagcgcatc gtcttatgac ttggaaaaaa
    10081 gaatacgcga ataacgcagt atttgttgac tgtgtagaag atttatatca aacgccgacc
    10141 attgcggcta aagcgaattt gatgagtaaa ttgaatgatt gtggtgcaaa ccctatttat
    10201 acacatatac caaatccaat ttgttataac aaggaggtat cgtattgtga atcaaacaat
    10261 tcttaatcgt gtaaagacta gagtaatgca tcaactggta tcatcactta tttatgagaa
    10321 tattgttgtg tataaagcgt catatcaaga cggtgtcggt cattttacaa tagaaggaca
    10381 tgattcagag tatcgtttta ctgctgaaaa gacacatagc tttgatcgta tacgtatcac
    10441 atcaccaatt gagcgtgtcg taggagatga ggcagataca acaacagact atacacaatt
    10501 attgagagag gctgtattta catttcctaa aaatgatgaa aagctagaac aatttattgt
    10561 tgagttatta cagacagaat taaaagatac acaaagtatg cagtatcgag aatcaaaccc
    10621 accagcaaca cctgagacat ttaacgacta tgaattttat gcgatggaag ggcatcagta
    10681 tcatccaagt tacaaatcac gtttaggatt tacgttgagt gataatttga aatttggtcc
    10741 tgattttgta ccaaacgtta aactgcagtg gttagctatc gacaaagata aagtagaaac
    10801 gacggtatca agaaatgttg tagttaacga aatgttacgt caacaagttg gcgataagac
    10861 ttatgaacat tttgtacagc aaattgaagc gtctggcaaa catgtaaatg atgttgagat
    10921 gatacctgta cacccatggc agtttgaaca tgtcatccaa gttgatttgg ctgaagaaag
    10981 gcttaatggc acagtactat ggttagggga aagtgatgag ctatatcacc ctcaacaatc
    11041 gattcgtacg atgtcgccaa tagacacgac aaaatattat ttaaaggtac caataagtat
    11101 aacgaacact tcaacgaaac gagtgttggc gcctcataca attgaaaatg cagcgcaaat
    11161 tacggattgg ttaaagcaga tacagcaaca agatatgtat ttaaaagatg aattaaagac
    11221 agtttttcta ggggaagtct taggacagtc ttatttaaat acacaacttt cgccttataa
    11281 acaaactcaa gtttatggtg cgttaggtgt tatatggcgt gaaaatatat atcatatgtt
    11341 aatcgatgaa gaggatgcga taccatttaa tgcactttat gcaagtgata aggatggttt
    11401 accattcatt gaaaagtgga ttaaacaata tggttctgaa gcttggacaa agcaattttt
    11461 agctgtagcg attcgtccaa tgattcatat gctttattat cacggtattg cctttgaatc
    11521 gcatgcacaa aatatgatgc tcattcatga aaatggttgg cctacacgta ttgccttaaa
    11581 agatttccac gatggtgttc gttttaagcg tgagcattta agtgaagcag cttcacacct
    11641 gacattaaag ccaatgccag aagcacataa aaaagtgaat agtaattcat ttattgaaac
    11701 agatgacgaa cgtttagtac gcgacttttt acatgatgca tttttcttta ttaatatcgc
    11761 cgaaatcatc ttatttattg aaaagcaata tggtatcgat gagcagcgac aatggcaatg
    11821 ggttaaagac attatcgagg cgtatcaaga agcatttcca gagttgaata actatcaaca
    11881 tttcgatttg tttgaaccta cgattcaagt tgaaaagtta acgacacgtc gattattaag
    11941 tgactccgag ttaagaattc atcatgttac aaatccatta ggtgtaggag gtatcaatga
    12001 tgcaacaact atctctgaaa catagattaa acaatggtga ttcagtttat ggcattttta
    12061 attctatacc ggacccattg atgatcgagg ttatcgcagc aagcgggtat gactttgttg
    12121 tgattgatac agaacacgtg gcgattaatg atgagacact agcgcattta attcgtgcag
    12181 ctgaagcagc gcatattata ccaattgtac gtgtcactgc agtgatagat agagatatca
    12241 ttaaagtgtt agatatgggt gcgagaggta ttattgtgcc acacgttaaa gatcgtgaga
    12301 cagttgagca tattgtgaaa ttaagtcgtt attacccgca aggattaaga agtttgaatg
    12361 gtggtcgcat ggcaagattt ggacgtacac cattacttga tgcaatggag atggctaatg
    12421 agcatattat ggtgattgcc atgatagaag atgttgaagg ggttatggcc attgacgata
    12481 tagctcaagt cgaaggttta gacatgatag tcgaaggtgc cgcagattta tcgcagtcac
    12541 ttggcatacc atggcaaacg cgtgatgatc aagtaacatc acatgttcaa catatttttg
    12601 aggttgtgaa tgcacatggt aaacattttt gtgcattacc acgtgaagat gaagatattg
    12661 caaaatggca ggcacaaggt gtacaaacat ttattttagg tgatgatcgc ggaaaaatat
    12721 atcgccattt aagtgcatct ctagcgacgt ctaaacagaa aggggatgat ggctaatgcg
    12781 tatagttcaa cctgttattg aacaattaaa agcacaatct catccagttt gtcattatat
    12841 ctatgattta gtcggactgg aacatcattt gcaacatatt acatcgtcat tgccgagtaa
    12901 ttgtcaaatg tactatgcaa tgaaagcaaa tagtgaacga acaatcctag atacaattag
    12961 tcagtatgtt gaaggattcg aagttgcatc tcaaggtgaa atagcaaaag gtcttgcttt
    13021 taaaccagca aatcatatta tttttggtgg ccctggtaag acagacgagg aactaagata
    13081 tgcagtaagt gaaggtgttc agcgtattca tgttgaaagt atgcatgaat tacaacggct
    13141 aaatgccatc ttagaagatg aagataagac acaacacatt ttattgcgtg ttaatttagc
    13201 aggaccattt cccaatgcaa cgttgcatat ggcaggacgc ccaacacaat ttggtatttc
    13261 tgaagacgaa gttgatgatg tcattgaagc tgcgctagta atgccaaata ttcatctaga
    13321 tggctttcat tttcattcta tttctaacaa tttagactcg aatttacatg tcgatgtagt
    13381 gaaactttat tttaaaaaag caaaatcatg gtctgaaaaa catcgatttc cactcaaaca
    13441 tatcaatctt ggtggtggca taggcgttaa ctatgcagat ttaactagcc aatttgagtg
    13501 ggataatttt gtagaaaatt ttaaaacact tatcgttgag caagaaatgg aagatgtgac
    13561 attgaacttt gaatgtgggc gctttattgt ggcacatatt ggttactatg tgacagaagt
    13621 gctagatatt aagaaagtgc atggtgcttg gtatgccatt ttaagaggag gtacgcaaca
    13681 atttagactg ccggtatctt ggcagcataa ccatcctttt gaaatttatc gttataagga
    13741 caatccatat tcatttgaaa aagtttcaat ttcgagacag gacacaacgt tagtcggtca
    13801 attatgtaca ccgaaagatg tctttgctag agaagtacag atagacgcaa tcagtacagg
    13861 cgacgttatt gttttcaaat atgcaggtgc atacggatgg tctatttcac atcacgattt
    13921 cttaagccat ccacatcctg aatttattta tttaacgcaa acaaaggagg atgaataact
    13981 attgaatcat attcatgaac atttaaaatt ggtaccagta gataagattg atcttcacga
    14041 aacattcgaa cctttaagat tggaaaaaac gaaaagtagt attgaagcag atgattttat
    14101 acgtcatcct attttagtga cagcgatgca acatggtaga tatatggtta tagatggtgt
    14161 gcatcggtat acaagtttga aagcgttagg atgtaagaaa gttccagtgc aagaaatcca
    14221 tgaaacacaa tattcaatta gtacatggca acataaagtt ccatttggtg tgtggtggga
    14281 aacgttacaa caagaacatc gcttgccatg gactactgag acaagacaag aagcgccatt
    14341 tattacgatg tgtcatggtg atacagaaca atatttgtat acgaaagatt taggcgaagc
    14401 acattttcaa gtatgggaaa aggttgtcgc aagttatagt ggttgttgtt ctgtagagag
    14461 aattgcacaa ggtacatatc cttgtctttc tcaacaagat gtactcatga agtatcagcc
    14521 attgagttat aaggaaattg aagcggttgt tcataaaggg gaaactgtgc cagcaggtgt
    14581 gacacgcttt aatatttcag gacgatgtct taatcttcaa gtaccactgg cattacttaa
    14641 acaagatgat gatgttgaac aactgcgtaa ttggaagcag tttttagcag ataagtttgc
    14701 caatatgaga tgctatactg aaaaagtata cttggtggag caatagtttt actgtgatgt
    14761 tgagggaaat atgatgattt agcgtattga tagcgaaaat ataataaaac aatatagtgt
    14821 ggagaacttt tgatatttta taaatattga agttctccat ttttgtattt tgcatataaa
    14881 aattaaataa aataaggtat attaaggtaa agtataaata ttaaataaat ggggaataga
    14941 catggaaaaa aatgtagaaa aatcattcat aaagataggt ttatattttc aaatagctta
    15001 tatagtactc atggctataa ctttatgtgg gtttgtaatt tgctatggac taattttcgg
    15061 ccttttctat ttattatcag gtagcagagc tgattattta atagtaacaa tagttatatc
    15121 ggcaataatt tctatatttg taattatact ttcaatcgta cctgtcatcg tattggcatc
    15181 tgacttattt aaagaaagga tttcaaaagg tgtcatatta attgtattgg ctattattgc
    15241 tttagtatta tgcaactttg tatctgcaat actctggttt gtttcagcca tatctatttt
    15301 aggtagaaaa aaattagtag ctgcagcaga tactaccact attcaaaaaa gtaaagggaa
    15361 cgcaaatcaa gcatcacata aagacacgtg taaaaaggaa cttgatagtc aagacatgat
    15421 ggaacatcct gaggttaaaa atcccacgac taaaaacctt gaaggattta acgaagaaat
    15481 acataaagat gaagctacaa ctaaagttgt cagtgataac acggaaccgc cgattgaatc
    15541 aaaaggccat gtctcgaaaa aagattgatg acaaactaat cgagagactt aaaaaaataa
    15601 tattcaacat aagaactttt aaaacgacat ttaaacgcat tgccaatcac taatggtagt
    15661 gcgtttaact ataccttaaa tatctgaata ttttgttaaa tggagctacc tttgttgtac
    15721 tattcaaatg aagaggagta aaatgtaatt aaaggaaaga aatttgagga gtgatcttta
    15781 tgacaaacaa caaagtagca ttagtaactg gcggagcaca agggattggt tttaaaattg
    15841 cagaacgttt agtggaagat ggtttcaaag tagcagttgt tgatttcaat gaagaagggg
    15901 caaaagcagc tgcacttaaa ttatcaagtg atggtacaaa agctattgct atcaaagcag
    15961 atgtatcaaa ccgtgatgat gtatttaacg cagtaagaca aactgccgcg caatttggcg
    16021 atttccatgt catggttaac aatgccggcc ttggaccaac aacaccaatc gatacaatta
    16081 ctgaagaaca gtttaaaaca gtatatggcg tgaacgttgc aggtgtgcta tggggtattc
    16141 aagccgcaca tgaacaattt aaaaaattca atcatggcgg taaaattatc aatgcaacat
    16201 ctcaagcagg cgttgagggt aacccaggct tgtctttata ttgcagtaca aaattcgcag
    16261 tgcgaggttt aacacaagta gccgcacaag atttagcgtc tgaaggtatt actgtgaatg
    16321 cattcgcacc tggtatcgtt caaacaccaa tgatggaaag tatcgcagtg gcaacagccg
    16381 aagaagcagg taaacctgaa gcatggggtt gggaacaatt tacaagtcag attgctttgg
    16441 gcagagtttc tcaaccagaa gatgtttcaa atgtagtgag cttcttagct ggtaaagact
    16501 ctgattacat tactggacaa acaattattg tagatggtgg tatgagattc cgttaataat
    16561 catgcattag cgataaataa atccttattg ttaagtttaa tcacttagca gtaaggattt
    16621 tttagtgcac ttagaaggga ttgtattggt agaaaattaa taagcgaagt tcttaaatga
    16681 gttatgatgt cacagtctaa tgcatcagtt gaaagcatta ctagtattaa cacacccaag
    16741 atattataaa acatcacaaa aacaccacta tctaatttat ctcaataaaa attcacaaag
    16801 ttatctcatt ttatttttat aaataaaaaa tatcgataaa aagcttacaa tactttatgt
    16861 ttttatgata tatttttaat gtataaatga ggtggaagat ttggaaagag ttttgataac
    16921 tggtggggct ggttttattg ggtcgcattt agtagatgat ttacaacaag attatgatgt
    16981 ttatgttcta gataactata gaacaggtaa acgagaaaat attaaaagtt tggctgacga
    17041 tcatgtgttt gaattagata ttcgtgaata tgatgcagtt gaacaaatca tgaagacata
    17101 tcaatttgat tatgttattc atttagcagc attagttagt gttgctgagt cggttgagaa
    17161 acctatctta tctcaagaaa taaacgtcgt agcaacatta agattgttag aaatcattaa
    17221 aaaatataat agtcatataa aacgttttat ctttgcttcg tcagcagctg tttatggtga
    17281 tcttcctgat ttgcctaaaa gtgatcaatc attaatctta ccattatcac catatgcaat
    17341 agataaatat tatggcgaac ggacgacatt aaattattgc tcgttatata acataccaac
    17401 agcggttgtt aaatttttta atgtatttgg gccaagacag gatcctaagt cacaatattc
    17461 aggtgttatt tcaaagatgt tcgattcatt tgagcataac aagccattta cattttttgg
    17521 tgacggactg caaactagag attttgtata tgtatatgat gttgttcaat ctgtacgctt
    17581 aattatggaa cacaaagatg caattggaca cggttataac attggtacag gcacttttac
    17641 taatttatta gaggtttatc gtattattgg tgaattatat ggaaaatcag tcgagcatga
    17701 atttaaagaa gcacgaaaag gagatattaa gcattcttat gcagatattt ctaacttaaa
    17761 ggcattagga tttgttccta aatatacagt agaaacaggt ttaaaggatt actttaattt
    17821 tgaggtagat aatattgaag aagttacagc taaagaagtg gaaatgtcgt gaaaatgaca
    17881 ttgaagctgt ccataataat aagggttatg cctatcaaag aaaattagat gcactcgaag
    17941 aagtgagaaa aggctattac ccaattaaac gtgcgattga cttagtatta agtatcgttt
    18001 tattattttt aacatttccg attatgttca tattcgccat tgctatcgtc atagattcgc
    18061 caggaaaccc tatttatagt caggttagag ttgggaagat gggtaaatta attaaaatat
    18121 acaaattacg ttcgatgtgt aaaaacgcag agaaaaatgg tgcgcaatgg gctgataaag
    18181 atgatgatcg tataacaaat gtcgggaagt ttattcgtaa aacacgcatt gatgaattac
    18241 cacaactaat taatgttgtt aaaggggaaa tgagttttat tggaccacgc ccggaacgtc
    18301 cggaatttgt agaattattt agttcagaag tgataggttt cgagcaaaga tgtcttgtta
    18361 caccagggtt aacaggactt gcgcaaattc aaggtggata tgacttaaca ccgcaacaaa
    18421 aactgaaata tgacatgaaa tatatacata aaggtagttt aatgatggaa ctatatatat
    18481 caattagaac attgatggtt gttattacag gggaaggctc aaggtagtct taatttactt
    18541 aataagttca aataaaagtt atattttaaa gattgtgacc aattgttaca gtataacgag
    18601 gaatcccttg agacagtatc aaatggcatt aagaaatatg tgccatcatt gatttgcatg
    18661 gctataaata ctattcatct gatgagatag ccatgttaag aaattgaaag tatagcatta
    18721 aaggggtttg taacagttga aaattatata ttgtattact aaagcagaca atggtggtgc
    18781 acaaacacat ctcattcaac tcgccaacca tttttgcgta caccatgatg tttatgtcat
    18841 tgtaggcaat catggaccaa tgattgaaca actagatgca agagttaatg taattattct
    18901 cgaacattta gtaggtccaa ttgactttaa acaagatatt ttagctgtca aagtgttagc
    18961 acagttattc tcgaaaatta agcctgatgt tatccattta cattcttcca aagctggaac
    19021 ggtcggacga attgcgaagt tcatttcgaa atcgaaagac acacgtgtag tttttactgc
    19081 gcatggatgg gcttttacag agggtgttaa accagctaaa aaatttctat atctagttat
    19141 cgaaaaatta atgtcatgta ttacagatag cattatttgt gtttcagatt tcgataaaca
    19201 gttagcgtta aaatatcgat ttaatcgatt gaaattaacc acaatacata atggtattgc
    19261 agatgttccc gctgttaagc aaacgctaaa aagccaatca cataacaata ttggcgaagt
    19321 agttggaatg ttgcctaata aacaagattt acagattaat gccccgacaa agcatcaatt
    19381 tgttatgatt gcaagatttg cttatccaaa attgccacaa aatctaatcg cggcaataga
    19441 gatattgaaa ttacataaca gtaatcatgc gcattttaca tttataggcg atggacctac
    19501 attaaatgat tgtcagcaac aagttgtaca agctgggtta gaaaatgatg tcacattttt
    19561 gggcaatgtc attaatgcga gtcatttatt atcacaatac gatacgttta ttttaataag
    19621 taagcatgaa ggtttgccaa ttagcattat agaagctatg gctacaggtt tgcctgttat
    19681 agccagtcat gttggcggta tttcagaatt agtagctgat aatggtatat gtatgatgaa
    19741 caaccaaccc gaaactattg ctaaagtcct ggaaaaatat ttaatagaca gtgattacat
    19801 caaaatgagt aatcaatcta gaaaacgtta tttagaatgt tttactgagg agaaaatgat
    19861 taaagaagtg gaagacgttt ataatggaaa atcaacacaa tagtaaatta ctaacattgt
    19921 tacttatcgg tttagcggtt tttattcagc aatcttcggt tattgccggt gtgaatgttt
    19981 ctatagctga ctttatcaca ttactaatat tagtttattt actgtttttc gctaaccatt
    20041 tattaaaggc aaatcatttt ttacagtttt tcattatttt gtatacatat cgtatgatta
    20101 ttacgctttg tttgctattt tttgatgatt tgatatttat tacggttaag gaagttcttg
    20161 catctacagt taaatatgca tttgtagtca tttatttcta tttagggatg atcatcttta
    20221 agttaggtaa tagcaaaaaa gtgatcgtta cctcttatat tataagcagt gtgactatag
    20281 gtctattttg tattatagct ggtttgaaca agtccccttt actaatgaaa ttgttatatt
    20341 ttgatgaaat acgttcaaaa ggattaatga atgaccctaa ctatttcgcg atgacacaga
    20401 ttattacatt ggtacttgct tacaagtata ttcataatta catattcaag gtccttgcat
    20461 gtggtatttt gctatggtct ttaactacaa cggggtctaa gactgcgttt atcatattaa
    20521 tcgtcttagc catttatttc tttattaaaa agttatttag tagaaatgcg gtaagtgttg
    20581 tgagtatgtt agtgattatg ctgatattac tttgttttac cttttataat atcaactact
    20641 atttattcca attaagcgac cttgatgccg taccgtcatt agatcgaatg gcgtctattt
    20701 ttgaagaggg ctttgcatca ttaaatgata gtgggtctga gcgaagtgtt gtatggataa
    20761 atgccatttt agtaattaaa tatacactag gttttggtgt cggattagtg gattatgtac
    20821 atattggctc gcaaattaat ggtattttac ttgttgccca taatacatat ttgcagatct
    20881 ttgcggaatg gggcatttta ttcggtgcat tatttatcat atttatgctt tatttactgt
    20941 ttgaattatt tagatttaac atttctggga aaaatgtaac agcaattgtt gtaatgttga
    21001 cgatgctgat ttacttttta acagtatcat ttaataactc aagatatgtc gcttttattt
    21061 taggaattat cgtctttatt gttcaatatg aaaagatgga aagggatcgt aatgaagagt
    21121 gattcactaa aagaaaatat tatttatcaa gggctatacc aattgattag aacgatgaca
    21181 ccactgatta caatacccat tatttcacgt gcatttggtc ccagtggtgt gggtattgtt
    21241 tcattttctt tcaatatcgt gcaatacttt ttgatgattg caagtgttgg cgttcagtta
    21301 tattttaata gagttatcgc gaagtctgtt aacgacaaac ggcaattgtc tcagcagttt
    21361 tgggatatct ttgtcagtaa attattttta gcgttaacag tttttgcgat gtatatggtc
    21421 gtaattacta tatttattga tgattactat cttattttcc tactacaagg aatctatatt
    21481 ataggtgcag cactcgatat ttcatggttt tatgctggaa ctgaaaagtt taaaattcct
    21541 agcctcagta atattgttgc gtctggtatt gtattaagtg tagttgttat ttttgtcaaa
    21601 gatcaatcag atttatcatt gtatgtattt actattgcta ttgtgacggt attaaaccaa
    21661 ttacctttgt ttatctattt aaaacgatac attagctttg tttcggttaa ttggatacac
    21721 gtctggcaat tgtttcgttc gtcattagca tacttattac caaatggaca gctcaactta
    21781 tatactagta tttcttgcgt tgttcttggt ttagtaggca cataccaaca agttggtatc
    21841 ttttctaacg catttaatat tttaacggtc gcaatcataa tgattaatac atttgatctt
    21901 gtaatgattc cgcgtattac caaaatgtct atccagcaat cacatagttt aactaaaacg
    21961 ttagctaata atatgaatat tcaattgata ttaacaatac ctatggtctt tggtttaatt
    22021 gcaattatgc catcatttta tttatggttc tttggtgagg aattcgcatc aactgtccca
    22081 ttgatgacca ttttagcgat acttgtatta atcattcctt taaatatgtt gataagcagg
    22141 caatatttat taatagtgaa taaaataaga ttatataatg cgtcaattac tattggtgcg
    22201 gtaatgaatt tagtattatg tcttgttttg atatattttt atggaattta cggtgcggct
    22261 attgcacgtt taattacaga gtttatctta cttatttggc gatttgttga tattactaaa
    22321 atcaatgtga agttgaatat tgtaagtacg attcaatgtg tcattgctgc cgttatgatg
    22381 tttattgtgc ttggtgtggt caatcattat ttgcccccta caatgtacgc tacgctgcta
    22441 ttaattgcga ttggtatagt agtttatctt ttattaatga tgactatgaa aaatcaatac
    22501 gtatggcaaa tattgaggca tcttcgacat aaaacaattt aagtatcggt aacgctatac
    22561 tttagaaatt taagattaag aagaaaaggc aatttcttat tgaaaaatgg aagttgtctt
    22621 ttttaattct ctttaaaagc gggaaacaaa agcagttaaa tgcctttttg cattcaatat
    22681 taaatattat atcaatttcg aatatttaaa ttttatataa ttggatataa caaataaata
    22741 ataattattg caaaacacac ccgaaattaa ttattataaa agtatattca taaaaggagg
    22801 aatatactta tggcatttaa attaccaaat ttaccatatg catatgatgc attggaacca
    22861 tatatagatc aaagaacaat ggagtttcat cacgacaaac atcacaatac gtacgtgacg
    22921 aaattaaacg caacagttga aggaacagag ttagagcatc aatcactagc ggatatgatt
    22981 gctaacttag acaaggtacc ggaagcgatg aggatgtcag tccgtaataa tggcggtggt
    23041 cattttaacc attcattatt ctgggaaata ctatcaccta attctgaaga aaaaggtggc
    23101 gtaatagatg acatcaaagc gcagtggggc actttagatg aatttaaaaa tgaatttgca
    23161 aataaagcaa caacattatt tggatcaggt tggacttggt tagttgttaa tgatggaaaa
    23221 ttagaaattg tgacaacgcc aaaccaagat aatccattaa cagaaggcaa aacaccaatc
    23281 ttactatttg atgtttggga gcatgcctac tatctgaaat atcaaaataa acgtccagac
    23341 tatatgactg cattttggaa tattgttaac tggaaaaaag ttgatgaatt ataccaagca
    23401 gcaaaataat ataacttaat ataattgagg tggagcattt acaaggtgtt ccactttttt
    23461 gtacttaata tctttactta tttgtttttg tgtaatggga tagcacgtaa aagtggcaaa
    23521 ataataagat aaatttcaac tcgaaatacc ttattattgt tgactataat gatgacctaa
    23581 atgttatata catcagacaa cactattaat taatcaacat attgacatta aataaattga
    23641 caaaataagt aattattgtg aatctaaagt gaaattttta taaaaaattg taacgattca
    23701 aaaattttgt tgcatttctt ttgtaatcat atgataatgt aaatgtaatc aaattgtaat
    23761 ataagggaaa agacaatgaa aaaattagca acagtaggtt ctttaattgt aacaagcact
    23821 ttagtattct caagtatgcc ttttcaaaat gcgcatgccg acacgacttc aatgaatgtg
    23881 ccgaataaac aaagccaaaa tgtacaaaat catcgccctt atggcggagt agtaccacaa
    23941 ggaatgacgc aagcacaata tactgaatta gagaaaactt taccccaatt aagcgctggc
    24001 agtaatatgc aagactataa tatgaaattg tatgatgcga cgcaaaatat tgctgataaa
    24061 tacaatgtga taattacaac taatgtaggg gtatttaaac cacatgctgt tagagatatg
    24121 aatggccatg cgttaccttt aacaaaagat ggcaattttt atcaaacgaa tgtagatgca
    24181 aatggtgtta atcatggtgg tagtgaaatg gtgcaaaata aaacaggtca tatgagtcaa
    24241 caagaccata tgaatcagaa cacacacatg aaccaacagc cacaaataca acaaggtcat
    24301 atgcaatcat caaaccatca aatgatgagt ccaaaagcaa atatgcattc atcaaatcat
    24361 caaatgaacc aaagtaacaa aaaagtttta ccagctgctg gtgaaagtat gacatcaagt
    24421 attcttactg caagtattgc cgcactacta ttagtatctg ggttattctt agcatttaga
    24481 cgacgttcaa caaataaata aacataatac gattaataat agaaaaatcg tgtgattatc
    24541 tgagcgagcc taggacataa atcaatgtcc taggctcgct aatgttatat tggcagtagt
    24601 tgactgaatg aaattgcgct tgtaacaagc ttttccattt cttaccaact ccctaaatag
    24661 tcatcaaaaa atttcttata ttttaataat tttaataatc cgattgtctt atacgtgtca
    24721 gtgttaattc agatatttcc tgtggaatat accacttatt aatcataatt ggataaggtg
    24781 tttgtgcgta cagtgtttca ataatcagcc aacaatgtgt atcaccatca aacacgtgac
    24841 tatgattttt gaagtggggc gctttggtaa tagacatttt taaatctgat tgatatgcat
    24901 tgctataaat cgtttgctca acgaatgtct tcatgtcgtc ttcgttttgt gtattcactt
    24961 taaatgtgtc aatgacattt aacggtataa aggtaaagca aaatgcatca gcttgcttag
    25021 aatgattgtc ctttttttga taatagcgtt ccattgcaat gacggcagaa ggatggtttg
    25081 caaacaaatg atttgtatat tcactttcta aatcaacacg ataattaatt gatgacatag
    25141 atacgcgagc tagcaatatt tgatcaagtg gatgcttaaa ttgatccata cttgaagcgt
    25201 gttgggcatt tgtttgtgga ataacaaagt gtcccttccc tcttgtactc tctacgatgc
    25261 catcttcggc taacaatttt atagcttggc gcaaagtcat acgactgaca tcaaagcgcg
    25321 cacaaagttc cttttcagta ggtaatgcat ggccactcgg atattttcct atttgaattt
    25381 ctttatataa cgtattataa atcgttaaaa attttggttg tgtttgcgtc acgtagacaa
    25441 cctccataaa gttacttaat cactctcatc atacaataat ttttactcga attggaaaaa
    25501 ttataaaaat taaatataga taggctttga aaattagttt tatacaaggt tagtagctgt
    25561 aactgtaaaa tgttcttaat attgtcaaaa tgtaatgctt gaaagcgctt ttaaaaaata
    25621 ttattatata catgattaga caaatagaca aatcactata caaatattgg gaggaatatt
    25681 ttatgaaatc aacaccacac attaaaccaa tgaatgacgt cgaaattgca gaaacggttc
    25741 tattgccagg agatccgtta agagctaagt tcattgcaga aacttatttg gatgatgtgg
    25801 aacagttcaa tacagtgcga aacatgtttg gttttaccgg aacatataaa ggtaaaaaag
    25861 tttctgtcat gggttcaggt atgggtatgc catctattgg catttactct tatgaattaa
    25921 ttcatacatt tggttgtaaa aaattaattc gcgttggctc ttgtggcgcg atgcaagaaa
    25981 acattgattt atatgatgtg attattgcac aaggtgcctc tactgattca aattacgttc
    26041 aacaatatca attaccaggt cattttgcgc caattgcttc ttatcaatta ttagaaaaag
    26101 cagttgaaac agcacgtgac aaaggtgtac gtcatcatgt aggtaatgtg ttatcaagtg
    26161 atattttcta taacgcggat acaacagcga gtgaacgttg gatgcgtatg ggtattttag
    26221 gtgtagaaat ggaatcagct gcattataca tgaatgcaat ttacgctggt gtcgaagcat
    26281 taggtgtgtt cacagtgagc gatcatttaa ttcatgaaac gtcaacaaca cctgaggaaa
    26341 gggaacgtgc atttacagat atgattgaaa ttgcactgtc attggtgtag atgattatga
    26401 atgttgaata ttctaaaata aagaaagcag tacctatttt attattctta tttgtattca
    26461 gtttggttat agacaactca tttaaattga tttctgtagc cattgctgat gacttaaaca
    26521 tatctgtaac gacagtaagt tggcaagcga cattagccgg tttagtaatt ggtattggcg
    26581 ctgtagtata cgcttcatta tctgatgcca ttagtatacg cacactattt atttatggcg
    26641 tgatattaat cattatcgga tcaattattg gttacatttt ccaacatcaa ttcccattac
    26701 ttttagttgg acgtattatt caaactgccg gtttagctgc tgcagagaca ttatatgtga
    26761 tatatgttgc aaagtatctt tctaaagagg accagaagac ttaccttggc ttaagtacga
    26821 gcagttattc cttgtcatta gttatcggta cattatcagg tggatttatt tctacgtatt
    26881 tacactggac aaatatgttt ttaattgcat taatcgtagt atttacgttg ccattcctat
    26941 ttaaattatt accaaaagaa aataatacga ataaagctca tttagatttt gttggcttaa
    27001 ttctagtggc aactattgct acaacagtca tgctgtttat tacgaacttt aattggttat
    27061 atatgattgg tgccttaatt gcgattatcg tttttgcgct atatattaaa aatgcgcaac
    27121 gtccattagt aaataaatca tttttccaaa ataaacgtta tgcttcattt ttatttatag
    27181 tatttgtaat gtatgctatc caattgggtt atatttttac gttcccattc ataatggagc
    27241 aaatttatca tctgcaacta gacacaacat cactgttatt agtaccgggt tatatagtag
    27301 cagtcattgt tggtgcatta agtggtaaaa tcggcgaata tctgaattca aaacaagcga
    27361 ttatcacagc aattatttta atagcactga gcttgatttt acctgcattt gcagtaggta
    27421 atcacatttc aatcttcgtc atttctatga tattctttgc aggtagcttt gctttaatgt
    27481 atgcaccttt acttaacgaa gccattaaaa caatagatct taatatgaca ggtgtggcta
    27541 ttggttttta taatttaatt attaatgtgg cggtatctgt aggtattgcg attgctgcgg
    27601 ctctaatcga ttttaaagca ttaaatttcc caggcaatga tgcattaagt tcacatttcg
    27661 gtattatttt aattatttta ggtttaatga gtattgtcgg attagtttta ttcgtcatct
    27721 taaatcgttg gacacaatct gaaaaataaa tagatataaa ttcgcgagat atattcgtat
    27781 ttatagtaaa attaaataaa gagattatat aacacgagga gtagtaagta tgaaatttga
    27841 gaaatatata gatcacactt tattgaagcc tgagtcaaca cgtacgcaaa tcgatcaaat
    27901 catcgatgaa gcgaaagcat acaattttaa atctgtatgt gtgaatccaa cgcatgttaa
    27961 atatgcagca gagcgactag ctgattcaga ggtgctcgtt tgtacggtaa taggattccc
    28021 attaggtgcg tcgacaactg caacgaaagc atttgaaaca gaagatgcaa ttcaaaatgg
    28081 tgcagatgaa attgacatgg tcatcaacat cggcgcatta aaagatggac gttttgatga
    28141 tgtacaacaa gacattgaag cagtggttaa agctgcgaaa ggtcacacag taaaagtgat
    28201 tattgagacg gtattgttgg accatgacga aattgtaaaa gcgagtgaat taacaaaag
//
LOCUS       DAOBBH010000014        28098 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.014, whole genome shotgun sequence.
ACCESSION   DAOBBH010000014 DAOBBH010000000
VERSION     DAOBBH010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 28098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28098
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.014"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            168..2243
                     /gene="lip2"
                     /locus_tag="P6L01_000415"
     CDS             168..2243
                     /gene="lip2"
                     /locus_tag="P6L01_000415"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip2/Geh"
                     /protein_id="HDP3029511.1"
                     /translation="MLRGQEERKYSIRKYSIGVVSVLAATMFVVSSHEAQASEKTPTS
                     NAAAQKETLNQPGEQGNAITSHQMQSGKQLDDMHKENGKSGTVTEGKDTLQSSKHQST
                     QNSKTIRTQNDNQVKQDSERQGSKQSHQNNATNNTERQNDQVQNTHHAERNGSQSTTS
                     QSNDVDKSQPSIPAQKVLPNHDKAAPTSTTPPSNDKTAPKSTKAQDATTDKHPNQQDT
                     HQPAHQIIDAKQDDTVRQSEQKPQVGDLSKHIDGQNSPEKPTDKNTDNKQLIKDALQA
                     PKTRSTTNAAADAKKVRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNK
                     FKVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGK
                     TYKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHKAHGGEISPLFTG
                     GHNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGF
                     KQLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLDGSAKLNNMTSMNPNITYTTYTGVS
                     SHTGPLGYENPDLGTFFLMDTTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFINVT
                     NDEPATRRGIWQVKPIIQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATE
                     SKGTQLKAS"
     gene            complement(2485..3312)
                     /locus_tag="P6L01_000416"
     CDS             complement(2485..3312)
                     /locus_tag="P6L01_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDP3029512.1"
                     /translation="MTEIKYKVITKDAFALPYTIIKAKNQPTKGVIVYIHGGGLMFGK
                     ANDLSPQYIDILTEHYDLIQLSYRLLPEVSLDCIIEDVYASFDAIQSQYSNCPIFTFG
                     RSSGAYLSLLIARDRDIDGVIDFYGYSRINTEPFKTTNSYYAKIAQSINETMIAQLTS
                     PTPVVQDQIAQRFLIYVYARGTGKWINMINIADYTDSKYNIAPDELKTLPPVFIAHCN
                     GDYDVPVEESEHIMNHVPHSTFERVNKNEHDFDRRPNDEAITIYRKVVDFLNAITMV"
     gene            complement(3385..4584)
                     /locus_tag="P6L01_000417"
     CDS             complement(3385..4584)
                     /locus_tag="P6L01_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDP3029513.1"
                     /translation="MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNASTKEGYITKADL
                     AYAARRSNSAGMQVTGAAYIEPYGKLFEYGFNIDHDACIPGLTNMASTMKQHGSLAII
                     QLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHVVIAMSHEKINSIIQQYRDATLRAI
                     KAGFDGVEISIAQRLLIQTFFSTFSNRRTDHYGADSLKNRARLCLEVMRAVQEVIDKE
                     APDNFILGFRATPEETRGSDLGYTIDEFNQLIDWVMDVSNIQYLAIASWGRHIYQNTS
                     RTPGDHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPD
                     FVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQ
                     NYHDSSS"
     gene            4680..4772
                     /locus_tag="P6L01_000418"
     CDS             4680..4772
                     /locus_tag="P6L01_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029514.1"
                     /translation="MENIFKIELMNGICRSENMNMKKKNKGEKI"
     gene            4886..5887
                     /locus_tag="P6L01_000419"
     CDS             4886..5887
                     /locus_tag="P6L01_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDP3029515.1"
                     /translation="MVKLSVLDYALIDEGKDAQKALQDSVTLAKLADRLGFKRIWFTE
                     HHNVPAFACSSPELLMMHTLAQTNHIRVGSGGVMLPHYRPYKIAEHFRMMAALYPNRI
                     DLGIGNNPGTTMVKQALDGINPTYDSYDESISLLRDYLIIKDKPSAHTLGVQPHIDHF
                     PEMWLLSSSATSAKIAAELGIGLSVGTFLLPDINAIHAAKDNIDIYKKHFQASTIKMD
                     AKVMASVFVIVADNEAEVAALQHALDVWLLGKLQFAEFEHFPSVDTAQKYKLNDRDKE
                     MIQVHQARIIAGTQEQVKAQLDDFIATFEVDEVLVAPLIPGIEQRCKTLKLLAEIYL"
     gene            5921..6253
                     /locus_tag="P6L01_000420"
     CDS             5921..6253
                     /locus_tag="P6L01_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDP3029516.1"
                     /translation="MKKLANYLWVEKVGDLYVFSMTPELQDDIGTVGYVEFVSPDEVK
                     VDDEIVSIEASKTVIDVQTPLSGTIIERNTKAEEEPTILNSEKPEENWLFKLDDVDKE
                     AFLALPEA"
     gene            6254..7054
                     /locus_tag="P6L01_000421"
     CDS             6254..7054
                     /locus_tag="P6L01_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HDP3029517.1"
                     /translation="METLKSNKARLEYLINDMRRERNDNDVLVMPSSFEDLWELYRGL
                     ANVRPALPVSDEYLAVQDAMLSDLNHQHVTDLKDLKPIKGDNIFVWQGDITTLKIDAI
                     VNAANSRFLGCMQANHDCIDNIIHTKAGVQVRLDCAEIIRQQGRNEGVGKAKKTRGYN
                     LPAKYIIHTVGPQIRRLPVSKMNQDLLAKCYLSCLKLADQHSLNHVAFCCISTGVFAF
                     PQDEAAEIAVRTVESYLKETNSTLKVVFNVFTDKDLQLYKEALNRDAE"
     gene            7044..7988
                     /locus_tag="P6L01_000422"
     CDS             7044..7988
                     /locus_tag="P6L01_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HDP3029518.1"
                     /translation="MQSSKWNAMSLLMDEKTKQAEVLRTAIDEADAIVIGIGAGMSAS
                     DGFTYVGERFTENFPDFIEKYRFFDMLQASLHPYGSWQEYWAFESRFITLNYLDQPVG
                     QSYLALKSLVEGKQYHIITTNADNAFDAAEYDMTHVFHIQGEYILQQCSQHCHAQTYR
                     NDDLIRKMVVAQQDMLIPWEMIPRCPKCDAPMEVNKRKAEVGMVEDAEFHAQLQRYNA
                     FLEQHQDDKVLYLEIGIGYTTPQFVKHPFQRMTRKNENAIYMTMNKKAYRIPNSIQER
                     TIHLTEDISTLITTALRNDSTTQNNNIGETEDVLNRTD"
     gene            7966..8988
                     /locus_tag="P6L01_000423"
     CDS             7966..8988
                     /locus_tag="P6L01_000423"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDP3029519.1"
                     /translation="MYLIEPIRNGEYITDGAIALAMQVYVNQHIFLDEDILFPYYCDP
                     KVEIGRFQNTAIEVNQDYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGD
                     FQRFYQPAIKALHTLGATDVIQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVN
                     YEAMDKVLKPNRKKIASKGIKSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGID
                     DIKEAKRYELTDADWEAIDELADKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISV
                     EQNRIADCRIYGDFFGQGDIKDVEEALQGTKMTREDLMHQLKQLDIVYHFGNVTVESL
                     VEMILS"
     gene            9304..10329
                     /locus_tag="P6L01_000424"
     CDS             9304..10329
                     /locus_tag="P6L01_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HDP3029520.1"
                     /translation="MNNKVLVTGGTGFVGMRIISRLLEQGYEVQTTIRDLSKADKVIK
                     TMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAI
                     EGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
                     LLAEKAAWDFVENENTIVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQI
                     PLNVVDVRDVAELHILAMTNEQANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVST
                     KKLPDFILSLGAKFNHQAKEGKLLLDMNRNVSNERAKTLLGWEPIATQEEAILAAIDS
                     MAKFHLI"
     gene            complement(10423..11769)
                     /locus_tag="P6L01_000425"
     CDS             complement(10423..11769)
                     /locus_tag="P6L01_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDP3029521.1"
                     /translation="MQAILNFIVDILSQPAILVALIAFIGLIVQKKPAATITSGTIKT
                     ILGFLILSAGADVVVRSLEPFGKIFQHAFGVQGIVPNNEAIVSLALKDFGTTAALIMV
                     CGMIVNILIARFTNLKYIFLTGHHTFYMAAFLAIILTVSHIKGWLTIVIGALVLGLIM
                     AVLPALLQPTMRKITGNDQVALGHFGSISYFAAGAVGQLFKGKSKSTEEIKFPKGLSF
                     LRESTISISITMALLYFIACLFAGVSYVHESISDGQNFIVFSLIQGVTFAAGVFIILT
                     GVRLILAEIVPAFKGISEKLVPNSKPALDCPIVFPYAQNAVLIGFFVSFITGVIGMFI
                     LFLFGGVVILPGVVAHFFLGATAAVFGNARGGIKGAVAGAALNGILITFLPLLFLPFL
                     GELGGAATTFSDTDFLTVGIVFGNAVKYMGLFGAILFIIIVGATAILLKGRQKPQQ"
     gene            complement(11784..12068)
                     /locus_tag="P6L01_000426"
     CDS             complement(11784..12068)
                     /locus_tag="P6L01_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDP3029522.1"
                     /translation="MKILVVCGHGLGSSFMVEMNAQEALRQLNAPSDIEVEHSDIMTA
                     SPEMADLFICGRDLAENAERLGDVLVLDNILDKAELQQKLSEKLQQLNMI"
     gene            complement(12070..12513)
                     /locus_tag="P6L01_000427"
     CDS             complement(12070..12513)
                     /locus_tag="P6L01_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDP3029523.1"
                     /translation="MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQA
                     MIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSLLKLNQHVAFCDEDHYASLIFVL
                     SAIDNHSHLSVLQNLATVLGDNQTVQQLLTAKNAQDIKNILKEHD"
     gene            complement(12518..14473)
                     /locus_tag="P6L01_000428"
     CDS             complement(12518..14473)
                     /locus_tag="P6L01_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDP3029524.1"
                     /translation="MLSYRQIEILYLLIHEQTFIPINTIANQLGVSPRTIQYDIAYIE
                     QYAETYHYQVSRNKAAGIKVTTAHATLLNELEHNLTNQIHFSKDERLTHIALKLFETT
                     DPVSTKQLAQDVNVSRRTIADDIKMIQAQLDQYHLKLNYVHNKGFNVIGEEDHYRKAY
                     AHFIHQYMKQAAPFIEADIFNSESIALVRRAIIKTLNSENYHLVQSAIDGLIYHILIA
                     IQRLNENFSFDIPINEIDKWRHTNQYAIASKMIENLERSCNVTFPESEIIFITLHLLG
                     SKMTEHTASSITFEYHDLSQNIHELITCVSQELGIDMSKDNKLHTSLITHIKPAIHRI
                     KYDMLQPNPLRQEVMRRYPQIIEAVSKHISPIEQDAAIRFNEDELTYITIHFASSIER
                     VATHKQSMIKVVLLCGSGIGTSQLLKSKLNHLYPEFHIWDAYSIYQLEESRLLQDNID
                     YVISTVPCEISAVPVIHVDPFINQQSRQKLNQIINDSREQRVMKMATDGKSLADLLPE
                     HRIIINEQPLSIESAIAVAVQPLINDGIVYSNYTAAILKQFEQFGSYMVISPHIALIH
                     AGTDYVQNGVGFALTYFTEGIIFGSKANDPVHLVITLATDHPNAHLKALGQLSECLSN
                     DLYRQDFLDGNIFKIKQHIALTMTKEV"
     gene            14685..15104
                     /gene="mepR"
                     /locus_tag="P6L01_000429"
     CDS             14685..15104
                     /gene="mepR"
                     /locus_tag="P6L01_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor MepR"
                     /protein_id="HDP3029525.1"
                     /translation="MEFTYSYLFRMISHEMKQKADQKLEQFDITNEQGHTLGYLYAHQ
                     QDGLTQNDIAKALQRTGPTVSNLLRNLERKKLIYRYVDAQDTRRKNIGLTTSGIKLVE
                     AFTSIFDEMEQTLVSQLSEEENEQMKANLTKMLSSLQ"
     gene            15211..16566
                     /gene="mepA"
                     /locus_tag="P6L01_000430"
     CDS             15211..16566
                     /gene="mepA"
                     /locus_tag="P6L01_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498905.1"
                     /note="MepA (multidrug export protein A), as described
                     originally in Staphylococcus aureus, is a MATE transporter
                     shown to be over-expressed after culturing in the presence
                     of tigecycline, owing to mutation of its repressor MepR,
                     and then to be one of several factors contributing to
                     elevated tigecycline resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MepA"
                     /protein_id="HDP3029526.1"
                     /translation="MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIG
                     FLEDSHMISAISLTLPVFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYG
                     GIALGLIVILVTLPFSDQIAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFAR
                     AIGAPMISMIGMLASVGLNIILDPILIFGFDLNVVGAALGTAISNVAAALFFIIYFMK
                     NSDVVSVNIKLAKPNKEMLSEIFKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASY
                     GISFRLVQFPELIIMGLCEGVVPLIAYNFMANKGRMKDVIKAVIMSIGVIFVVCMIAV
                     FTIGHHMVGLFTTDQAIVEMATFILKVTMASLLLNGIGFLFTGMLQATGQGRGATIMA
                     ILQGAIIIPVLFIMNALFGLTGVIWSLLIAESLCALAAMLIVYLLRDRLTVDTSELIE
                     G"
     gene            16670..17110
                     /locus_tag="P6L01_000431"
     CDS             16670..17110
                     /locus_tag="P6L01_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HDP3029527.1"
                     /translation="MYKSKILLKYIFSEESEVKDLTEEKYNQDYEALTFSFKEETYQS
                     RLAKKTPTKAGYFVTCWTKDENNCNQPYSKEAFADYLMIIVIDEELSGYFLFPRELLV
                     EKGILTTFEHKGKMAFRVYPKWCNQLNKTAGQTQKWQCKYFFEY"
     gene            complement(17193..18551)
                     /gene="glpT"
                     /locus_tag="P6L01_000432"
     CDS             complement(17193..18551)
                     /gene="glpT"
                     /locus_tag="P6L01_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDP3029528.1"
                     /translation="MNFLKPAKHIKSLPENQIDDTYKRLRLQVFLGIFIGYAGYYLLR
                     KNFSLAMPALQEQGFTKAELGFALSAVSIAYGFSKFFMGTVSDRSNARIFLVLGLALT
                     AIVNLLMGFVPFFTSGIGIMFVLLFLNGWFQGMGWPPSGRVLVHWFSVSERGSKTALW
                     NVAHNVGGGIMAPIAAWGITTTAFINFGYLKGFEGVFIYPALLALIIAAISYVLIRDT
                     PQSQGLPPIEIYKNDFATSDKKTLETELTTKEILFKYVLNNKWVWAIAFANIFVYFVR
                     YGVLDWAPVYLSEEKHFDLKASGWAYFLYEWAGIPGTLLCGYISDKLFKGRRGPAGFF
                     FMLGVTVFVLIYWLNPPGNAWLDHVSLIAIGFLIYGPVMLIGLQALDYVPKKAAGTAA
                     GLTGLFGYLFGAVMANIVLGAVVDKFGWDVGFILLTAISVFAMLSFILTWNKVGQETV
                     HH"
     gene            18863..19789
                     /locus_tag="P6L01_000433"
     CDS             18863..19789
                     /locus_tag="P6L01_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDP3029529.1"
                     /translation="MNIVGHHHISMYTKDAKRNKDFYTNVLGLRLVEKSVNQDNPSMY
                     HLFYGDEVGTAGTILSFFEIPHAGHKQPGTETIYRFSLLVPNQAALHYFEKRLDNNGI
                     TSERLYYLGQEGVVFKDEDDLEIILLVNDSFEVPHQWQHNAYSEIPQAYQILGIGPVE
                     LRVRNAARTVEFLENVLGYRKRDNKSFDVLTLAPQGLYSDFVVIEQQGQRERPGRGYI
                     HHIAINTPQMSDLDAIYKKLQQQTQSNSGIIDRYFFKSLYYRHNSIMYEFATEAPGFT
                     IDTPLEQLGSQLNLPDFLEAEREQIESKLHEI"
     gene            19803..20864
                     /locus_tag="P6L01_000434"
     CDS             19803..20864
                     /locus_tag="P6L01_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDP3029530.1"
                     /translation="MAKLEMNKNTPLEFGLYSLGDHLLNPFKGEKVSYEQRINEIIEA
                     SKLADETGIDVFAVGESHQEHFTTQAHTVVLGAIAQATKHIKVSSSSTIISATDPVRV
                     FEDFATLDLISHGRAEIVAGRASRTGIFDLFGYDLKDYDELFEEKLGLLLELNKTERI
                     TWSGKYRPELRNMKIFPRPIDNILPIWRAVGGPPASAIKAGKQGVPMMITTLGGPAMN
                     FKGSIDAYRQAATEAGFDASHKSLPVSTASLFYTAETTQDAMREFYPHLNTGMSFIRG
                     VGYPKQQFANSSDYREALMVGSPQQIIEKILYQHELYGHQRFMAQLDFGGVPFENVMK
                     NIELIGNEIIPAIKKHLSK"
     gene            20878..21444
                     /locus_tag="P6L01_000435"
     CDS             20878..21444
                     /locus_tag="P6L01_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDP3029531.1"
                     /translation="MNIVLLSGSTVGSKTRIAMDDLKNELEVINEGHQIELMDLRELE
                     LEFSVGKNYLDTTGDVYKLTTSLMQADVIFIGFPIFQASIPGALKNVFDLLPVNAFRD
                     KVIGLVATAGSSKHYLIPEMHLKPILSYMKAHTMQTYVFIEEKDFSNQQIVNDDVVFR
                     LKALAQFTMRTAKVQQQVFEEENNQYDF"
     gene            complement(21503..22498)
                     /locus_tag="P6L01_000436"
     CDS             complement(21503..22498)
                     /locus_tag="P6L01_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HDP3029532.1"
                     /translation="MASLKNKHFMIGLSLTFIVALFSFLAAKLPILDKVGALTIAILI
                     AILYRHFRGYPEQYSSGITFSSKYLLRFAIILYGLKLNIFDIIGQGSKLLAIDVGVVI
                     FSIVMMLFVNKLLHGDKNIALLLGVGTGVCGAAAIAAVAPIFKSREKDTAISIGIIAL
                     IGTIFSLIYTAIYAIFSMTTNVYGAWSGVSLHEIAHVVLAGGFGGSDALKIALLGKLG
                     RVFLLIPLTIVLILIMRFRSSESSSKGRISIPYFLIGFVIMALVNTYVTIPSVLLNIL
                     NTISTICLLMAMVALGLNVAFKDLKNRALKPLMTIIITSICLSSLAFIVVHWLYS"
     gene            22863..23408
                     /locus_tag="P6L01_000437"
     CDS             22863..23408
                     /locus_tag="P6L01_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDP3029533.1"
                     /translation="MFGMKVNEQITLKILEAHDTEALFNLVNRSRNSLREWLPWVDAT
                     EQPSDTRAFIKRGLLQFADGNGFQCGIWYEGTLVGVIGLHEINHMHRKTSLGYYLDKQ
                     FEGHGIMTQAVEALIKYCFEELDLNRIEISAAVNNEKSRAIPERLGFTREGMLRDNEL
                     LNGIYSSSYIYSLLKSEYDQK"
     gene            23675..24529
                     /locus_tag="P6L01_000438"
     CDS             23675..24529
                     /locus_tag="P6L01_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029534.1"
                     /translation="MKKLTTLLLASTLLIAACGNDDSKKDDSKTSKKDDGVKAELKQA
                     TKAYDKYTDEQLNEFLKGTEKFVKAIENNDMAQAKALYPKVRMYYERSEPVAEAFGDL
                     DPKIDARLADMKEEKKEKEWSGYHKIEKALYEDKKIDDVTKKDAQQLLKDAKELHAKA
                     DTLDITPKLMLQGSVDLLNEVATSKITGEEEIYSHTDLYDFKANVEGAQKIYDLFKPI
                     LEKKDKKLSDDIQMNFDKVNQLLDKYKDNNGGYESFEKVSKKDRKAFADAVNALGEPL
                     SKMAVITE"
     gene            24526..25755
                     /gene="efeB"
                     /locus_tag="P6L01_000439"
     CDS             24526..25755
                     /gene="efeB"
                     /locus_tag="P6L01_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDP3029535.1"
                     /translation="MTNYEQVNDSTQFSRRTFLKMLGIGGAGVAIGASGVGSMWSFKS
                     MFNTPEDPEKDAYEFYGKVQPGITTPTQKTCNFVALDLKSKDRDAIKAMFKKWTVMAD
                     RMMDGDTVGKTSNNPLMPPVDTGESIGLGASKLTITFGISKSLMKKIGLSSKIPDAFK
                     DLPHFPNDQLIDDYSDGDIMIQACSNDSQVSFHAVHNLVRPFRDIVKVRWAQSGFISA
                     KGKETPRNLMAFKDGTINPRKSNQLKDYVFIDDGWAKHGTYCVVRRIQIHIETWDRTA
                     LEEQEATFGRKRHSGAPLTGGKEFDEIDLKAKDSHGEYIIDKDAHTRLAKEANTSILR
                     RAFNYVDGTDDRTGNFETGLLFIAFQKATKQFIDIQNNLGSNDKLNEYITHRGSASFL
                     VLPGVSKGGYLGETLFD"
     gene            25736..27447
                     /locus_tag="P6L01_000440"
                     /pseudo
     CDS             25736..27447
                     /locus_tag="P6L01_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
     gene            complement(27688..>28098)
                     /locus_tag="P6L01_000441"
     CDS             complement(27688..>28098)
                     /locus_tag="P6L01_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HDP3029536.1"
                     /translation="YSFFSVLLFCAGVAFAFYVGFPIIIQFALKLSLTLNISPVIGFK
                     AYLVELIRWLFTFGILFQLPILFIGLAKFGLIDITSLKHYRKYIYFACFVLASIIAPP
                     DLTLNILLTLPLILLFEFSMFIVKFTCRGKPPTH"
BASE COUNT         9656 a         4273 c         5549 g         8620 t
ORIGIN      
        1 gaaatgcaca tatttgtaaa tgattagtat cgatttaata tcgtattatt aaatttttat
       61 taattttgta gtcttaatca aaaaataata tatgtcatgt tatattgaag gtgcagttgt
      121 ttttcattct caagaggggg tcaaaaaaat acttttgagg tgattatatg ttaagaggac
      181 aagaagaaag aaagtatagt attagaaagt attcaatagg cgtggtgtca gtgttagcgg
      241 ctacaatgtt tgttgtgtca tcacatgaag cacaagcctc ggaaaaaaca ccaacgtcca
      301 atgcagcggc acaaaaagaa acactaaatc aaccgggaga acaagggaac gcgataacgt
      361 cacatcaaat gcagtcagga aagcaattag acgatatgca taaagagaat ggtaaaagtg
      421 gaacagtgac agaaggtaaa gacacgcttc aatcatcgaa gcatcaatca acacaaaata
      481 gtaaaacaat cagaacgcaa aatgataatc aagtaaagca agattctgaa cgacaaggtt
      541 ctaaacagtc acaccaaaat aatgcgacta ataatactga acgtcaaaat gatcaggttc
      601 aaaataccca tcatgctgaa cgtaatggat cacaatcgac aacgtcacaa tcgaatgatg
      661 ttgataaatc acaaccatcc attccggcac aaaaggtatt acccaatcat gataaagcag
      721 caccaacttc aactacaccc ccgtctaatg ataaaactgc acctaaatca acaaaagcac
      781 aagatgcaac cacggacaaa catccaaatc aacaagatac acatcaaccc gcgcatcaaa
      841 tcatagatgc aaagcaagat gatactgttc gccaaagtga acagaaacca caagttggcg
      901 atttaagtaa acatatcgat ggtcaaaatt ccccagagaa accgacagat aaaaatactg
      961 ataataaaca actaatcaaa gatgcgcttc aagcgcctaa aacacgttcg actacaaatg
     1021 cagcagcaga tgctaaaaag gttcgaccac ttaaagcgaa tcaagtacaa ccacttaaca
     1081 aatatccagt tgtttttgta catggatttt taggattagt aggcgataat gcacctgctt
     1141 tatatccaaa ttattggggt ggaaataaat ttaaagttat cgaggaattg agaaagcaag
     1201 gctataatgt acatcaagca agtgtaagtg catttggtag taactatgat cgcgctgtag
     1261 aactttatta ttacattaaa ggtggtcgcg tagattatgg cgcagcacat gcagctaaat
     1321 acggacatga gcgctatggt aagacttata aaggaatcat gcctaattgg gaacctggta
     1381 aaaaggtaca tcttgtaggg catagtatgg gtggtcaaac aattcgttta atggaagagt
     1441 ttttaagaaa tggtaacaaa gaagaaattg cctatcataa agcgcatggt ggagaaatat
     1501 caccattatt cactggtggt cataacaata tggttgcatc aatcacaaca ttagcaacac
     1561 cacataatgg ttcacaagca gctgataagt ttggaaatac agaagctgtt agaaaaatca
     1621 tgttcgcttt aaatcgattt atgggtaaca agtattcgaa tatcgattta ggattaacgc
     1681 aatggggctt taaacaatta ccaaatgaga gttacattga ctatataaaa cgcgttagta
     1741 aaagcaaaat ttggacatca gacgataatg ctgcctatga tttaacgtta gatggctctg
     1801 caaaattgaa caacatgaca agtatgaatc ctaatattac gtatacgact tatacaggtg
     1861 tgtcttcaca tactggtcca ttaggttatg aaaatcctga tttaggtaca tttttcttaa
     1921 tggatacaac gagtagaatt attggtcatg atgcaagaga agaatggcgt aaaaatgatg
     1981 gtgtcgtacc agtgatttcg tcgttacatc catccaatca accatttatt aatgttacga
     2041 atgatgaacc tgccacacgc agaggtatct ggcaagttaa accaatcata caaggatggg
     2101 atcatgtcga ttttatcggt gtggacttcc tggatttcaa acgtaaaggt gcagaacttg
     2161 ccaacttcta tacaggtatt ataaatgact tgttgcgtgt ggaagcgact gaaagtaaag
     2221 gaacacaatt gaaagcaagt taaattcatc ttctgaattt aataggctat gtaaatcgtg
     2281 ctgttatcat ggcacatcag atataagtag catcacagtg ttgaatttca aaatagtaaa
     2341 gtgaaataaa gcgcctgtct cattagcgaa aactaaaggg acaggcgtat ctgtttatga
     2401 gcttaataaa ttgtatgaat aatatggttg atcgaataac tgtttatcat gatgataaat
     2461 tgagtttttt aaaataatga tatattacac cattgttata gcgtttaaga aatcaacaac
     2521 tttacgataa atagtgattg cttcgtcatt aggtctacga tcaaaatcat gctcgttttt
     2581 attcacgcgt tcaaatgttg aatgtggaac atgattcatg atatgttcgc tttcctcaac
     2641 gggaacatca taatcgccat tacaatgcgc aatgaaaaca ggtggaagtg ttttaagttc
     2701 atctggtgca atattatatt ttgaatcagt ataatcagca atgttaatca tatttatcca
     2761 tttacctgtg ccacgtgcat aaacgtagat taaaaaacgt tgtgcgattt gatcttgaac
     2821 aaccggtgtt ggtgaagtga gttgtgcaat cattgtttcg tttatgcttt gagctatttt
     2881 tgcgtaataa ctattagttg ttttaaaagg ttcagtgttg atgcgactat aaccataaaa
     2941 atcaataaca ccatcaatat ctctgtctcg tgcaattaat agacttaaat atgcacctga
     3001 tgatctgcca aaggtaaaaa tagggcaatt agaatattgt gattgaatcg catcgaatga
     3061 tgcgtagaca tcctcaataa tgcaatcgag acttacttct ggtaataaac gataacttag
     3121 ttgaattaaa tcgtaatgtt ccgtaaggat atcgatatac tgtggggata aatcgttagc
     3181 tttaccgaac attaatccac caccgtggat gtagacaata acgccttttg ttggttgatt
     3241 ttttgcttta ataattgtgt aaggtaatgc aaatgcatct ttagtaatta ctttatattt
     3301 aatttcagtc acgatttaat aggctcctta tttttgatat tgatgtcatt ataacactgt
     3361 cttaaatttc catgaaaaat agtcttaaga cgatgagtca tgataattct gttccaattg
     3421 acgtaaagcg tcacgggtat gcttctttag accttcccca taatccatca ttttaacaat
     3481 atctttaaaa gcagcatgtg gaatggctaa atcttctaaa tctgccatag aaaattcaag
     3541 attgatatca tgtggtcgct gttcagcaag tttatgcaca aagtcaggtt ctgtgacaaa
     3601 aggcgaagac atgccgacca tatctgcatg ttgtaaagca tctaaagcag actctggaga
     3661 attaatcccg ccacttgcaa ttaaagggat acgacctgct aaatgttcat agacaatttg
     3721 gttaactggt cgaccgaaat gatcacctgg tgtacgagac gtattttgat aaatatgtcg
     3781 accccagcta gcgattgcta agtattggat gtttgaaacg tccatgaccc aatcgattaa
     3841 ttggttgaac tcgtcaatgg tatatcctaa atcactgcct ctggtttctt ctggcgttgc
     3901 tcgaaatcct aaaataaaat tgtcaggtgc ttctttatca atcacttctt gtaccgcacg
     3961 cataacttct aaacataatc ttgcacgatt ttttaatgag tcggcaccgt aatggtctgt
     4021 acgtctattt gaaaaagttg agaaaaatgt ttgaatcagc aaacgttgtg caatcgaaat
     4081 ttccacacca tcaaaacctg ctttaatcgc gcgtaatgta gcatcgcgat actgctgaat
     4141 gatgctattg attttctcat gagacatggc gataacaacg tgttcaatcg gtgaatgcaa
     4201 agtcatagga cttggtccat acacctttcc aaaatttaaa atggcttgat ttgaaaaacg
     4261 accagcatgc gctagctgga taatagcgag gctaccatgt tgtttcatcg tagatgccat
     4321 gttagttaat ccagggatac aagcatcatg atcaatatta aagccatatt caaacaattt
     4381 accataaggt tcaatgtaag cagcgccggt gacttgcatt ccagctgaat tagagcgacg
     4441 tgcagcataa gccaagtctg cttttgtaat atagccttct tttgttgatg cgtttacggt
     4501 cattggtgat aatacaaagc gattcgaaat tttgatgcca ttaggtaagt ggattgattg
     4561 taaaagtggt ttgtatcggt acatactatg attccttttc tattcaatat tgttttcaaa
     4621 gtaccatgga aagaatgaat aatcaatgat gaacagtctt gatagaatag aattggtaca
     4681 tggaaaacat ttttaaaatt gaactaatga atggcatttg taggtctgaa aatatgaata
     4741 tgaaaaagaa aaataaaggc gaaaagatat aaaagttaat tgaaaaacgt tatcatatac
     4801 gtgggtatat gaagagggaa tggtattaag aacgctaaaa tgttatgtcg gtttgacatg
     4861 acaggataag tttggagatg acggattggt taaattaagc gtattagact atgccttaat
     4921 agatgaaggt aaggatgcac aaaaggcatt gcaagattca gtgacacttg caaaattagc
     4981 agatcgactt ggctttaagc gaatttggtt tacggaacat cataatgtac cagcgtttgc
     5041 gtgtagtagt ccagaacttt tgatgatgca tacattggcg cagacaaatc acatacgagt
     5101 tggctctggt ggtgtgatgc tgccgcacta tcgaccttat aaaattgctg agcattttag
     5161 aatgatggca gcgttatatc caaatcgtat tgatttaggt attggtaata atccaggtac
     5221 tactatggta aagcaagctt tagatggaat aaatcctaca tatgatagtt acgatgaatc
     5281 gatttcgtta ttacgtgatt atcttataat aaaggataaa ccaagtgcgc atacgttagg
     5341 tgtccaacca cacattgatc attttccaga aatgtggtta ttaagtagta gcgcaacatc
     5401 tgccaaaata gctgccgaac taggtatagg gctttctgtt ggaacatttt tgctaccaga
     5461 tataaatgcg atacatgcag cgaaggataa cattgatatt tacaaaaaac atttccaagc
     5521 atcaacgatt aaaatggacg caaaggtgat ggcatctgta tttgtcattg tagcagataa
     5581 cgaagcggaa gtagcagcat tgcaacatgc cttagatgtt tggttattag gtaaattaca
     5641 atttgcagaa tttgaacatt ttccttcagt agacacagca caaaagtata agcttaatga
     5701 tcgagacaaa gagatgattc aagtacatca agcacgcatc attgcaggca cacaagaaca
     5761 ggttaaagca caattagatg atttcattgc tacgtttgaa gttgatgagg tgttagtagc
     5821 accgcttatt ccaggtattg aacagcgttg taaaacatta aaattactcg cggaaattta
     5881 tttgtagcat tttaaataga agagaaagga tgaagataag atgaaaaagt tagccaatta
     5941 tttatgggta gaaaaagtag gagatttgta tgtgtttagt atgacacctg aattgcaaga
     6001 tgatattggg acagtaggtt atgttgaatt cgtaagtcca gatgaagtta aagtggatga
     6061 tgaaattgtg agtatcgaag catcgaaaac ggtcattgat gtgcaaacgc cattgtcagg
     6121 aacgattatt gagcgaaata caaaagcgga agaagaaccg acaattttaa actctgaaaa
     6181 accagaagaa aattggttgt tcaaattgga tgatgtcgat aaagaagcat tcctagcatt
     6241 accggaggct taaatggaaa cgttaaaatc aaataaagcg agacttgaat atttaatcaa
     6301 tgatatgcgt cgagagagaa atgacaatga cgtattggta atgccatctt catttgaaga
     6361 tttgtgggaa ttatatcgag gcttggcaaa tgtcagaccg gcattacctg taagtgatga
     6421 atatttagct gtacaagatg ctatgttaag tgatttgaat catcaacatg ttacggattt
     6481 gaaggatttg aagccgataa aaggtgacaa tatctttgtt tggcaaggtg atatcacgac
     6541 gttaaaaatc gatgctattg ttaatgctgc aaatagtcgt tttctaggat gtatgcaagc
     6601 taatcatgac tgcattgata atattattca tacaaaagcg ggtgttcaag ttcgacttga
     6661 ttgtgcagag atcattcgac aacaagggcg caatgaaggt gtaggtaaag ccaaaaaaac
     6721 acgtggatat aatttgccag caaagtatat aattcatacg gttggtccgc aaatacgtcg
     6781 attgcctgtt tcaaagatga atcaggactt gttagctaaa tgttatctta gctgtcttaa
     6841 attggctgat caacatagtt taaatcatgt cgctttttgc tgtatatcta caggtgtatt
     6901 tgcttttcct caagatgaag cagcagaaat tgctgttcga acagtagaaa gctatctcaa
     6961 agaaacaaat tcaacattga aagtcgtgtt caatgtattt acagataagg atttacaact
     7021 gtataaggag gcattgaacc gtgatgcaga gtagtaaatg gaatgcaatg tctcttttaa
     7081 tggatgaaaa gacaaagcag gctgaagtat tgcgtactgc gattgatgaa gcagatgcga
     7141 tagtgattgg aattggtgca ggcatgtctg catctgacgg atttacatat gtaggagagc
     7201 gttttacgga aaatttccca gattttattg aaaaatatcg cttctttgat atgttgcaag
     7261 cgagtttaca tccttatggc agttggcaag agtattgggc atttgagagt cgttttatta
     7321 cattaaatta tttagatcaa cctgtaggtc agtcttatct cgcattaaaa tccttagtgg
     7381 aaggtaaaca gtaccatatc ataactacga atgcagacaa tgctttcgat gctgctgaat
     7441 atgatatgac tcatgtattt catatacaag gggagtatat actgcaacag tgtagtcagc
     7501 attgtcatgc tcaaacgtat cgcaatgatg atttaattcg taaaatggtt gttgcgcaac
     7561 aagatatgct tataccttgg gagatgattc caagatgtcc aaaatgtgat gctccaatgg
     7621 aagtgaataa acgtaaagcg gaagttggca tggttgaaga tgctgaattt catgcgcaac
     7681 tacaacgtta taatgctttt ctagagcaac atcaagatga taaagtgttg tatttggaaa
     7741 ttggaattgg ttatactaca ccacaatttg tgaagcatcc ttttcagcgt atgacacgta
     7801 aaaatgaaaa tgcaatttat atgacgatga ataaaaaggc atatcgcatt ccgaattcaa
     7861 ttcaagaacg taccatacat ttaactgagg atatctcaac attgattaca acagcactcc
     7921 ggaacgacag tacaacgcaa aataacaaca ttggagagac agaagatgta cttaatagaa
     7981 ccgattagaa atggagaata tattactgat ggtgcgattg cactggctat gcaagtttat
     8041 gttaaccagc atatcttttt agatgaagat attttattcc cttattattg tgatccaaaa
     8101 gtggaaattg gacgttttca aaatactgct atagaagtga atcaagatta tatagataaa
     8161 cacagtattc aagtagttcg ccgagatact ggtggtggcg ctgtgtatgt tgataaaggt
     8221 gccgttaata tgtgttgtat tttagaacaa gacacttcaa tttatggtga ttttcaacga
     8281 ttttatcaac cagctataaa ggcattgcat acattaggtg caacagatgt gatacaaagc
     8341 ggtagaaatg atttaacatt gaacggcaaa aaagtgtcag gcgccgcaat gacattaatg
     8401 aacaatcgta tttatggcgg ttattcgcta ttacttgatg ttaattatga agcaatggat
     8461 aaagtgttaa agcctaatcg caaaaagatt gcatcgaaag ggattaaatc tgtgcgcgca
     8521 cgtgttggtc atcttagaga agcactggat gaaaagtatc gtgatataac cattgaagaa
     8581 tttaaaaatt taatggtgac gcagattttg ggaatcgatg acattaaaga ggcgaaacga
     8641 tatgaattaa cggatgcaga ttgggaagcg attgatgaat tagctgataa aaagtataaa
     8701 aattgggatt ggaattatgg gaagtcacct aaatatgaat ataatcgaag tgaaagatta
     8761 tcatcaggta ctgtagatat taccatttct gttgaacaaa atcgtatcgc agattgtcgt
     8821 atttatgggg atttctttgg acaaggtgat ataaaagatg tggaagaagc attacaagga
     8881 acaaaaatga caagagaaga tttaatgcat cagttaaagc aattagacat cgtttatcat
     8941 tttggtaatg ttacggtaga atcattggtt gagatgattt taagttaata ttgtttaggc
     9001 gttaatgtca caccctcaat gaatatgtgt atttgctttt aaaatggtga ctatgtagtt
     9061 atgtaatttg attatacgat tgaaaatgaa tttagttaat tagcaaagac atcatattta
     9121 tagcgtttgt gtttattagt gattgggtat ttgaaaatgg ttaatgatag tttttaaagg
     9181 aataaattca ttttcattta tataagtaag attaaatact agctactaaa gtctatttat
     9241 atacgtcaaa atataatgac tgctataatg agtaatcaat agacacaaag aggagattat
     9301 gtgatgaata ataaagtatt agtaaccggt ggtacagggt ttgttggcat gcgaattatt
     9361 tcacgattat tagaacaagg ttatgaggta caaacaacga tacgtgattt aagtaaagct
     9421 gataaagtaa ttaaaacaat gcaagacaat ggcatttcca cagagcgatt aatgtttgtc
     9481 gaagcggatt tatcacaaga tgaacattgg gatgaagcaa tgaaagattg taaatatgtc
     9541 ttgagtgtag catctccggt gtttttcggt aaaacagacg atgcagaagt gatggcgaag
     9601 cctgccattg aaggcataca acgtatttta agagctgcag aacatgctgg cgtgaagcgt
     9661 gtggtgatga ctgcaaactt tggtgcagtt ggttttagca ataaagataa aaattcaatc
     9721 acaaatgaaa gtcattggac aaatgaagat gaaccaggct tatcagtata tgaaaaatca
     9781 aaattgttag ctgaaaaggc agcgtgggat tttgttgaga atgaaaatac aatagtagaa
     9841 tttgccacaa tcaatccagt tgcaattttt ggaccatcat tagatgcaca cgtttcagga
     9901 agtttccact tgttagaaaa cttattaaat ggttcaatga aacgtgtacc gcaaattcca
     9961 ttgaatgttg ttgatgtgag agacgtagct gaactacaca ttttggcaat gacaaatgaa
    10021 caggctaatg gcaagcgatt tattgctaca gctgatggac aaattaattt gttggaaatt
    10081 gcaaaattaa ttaaagaaaa gagacctgaa atagctcaaa aagtttctac taaaaaatta
    10141 ccagacttta ttttgagtct aggtgctaaa tttaatcatc aagctaaaga aggtaaactt
    10201 ttattagata tgaaccgcaa tgtaagtaac gaacgtgcaa aaacattact tggttgggaa
    10261 ccgattgcga cacaagaaga agcaatttta gcagcaattg atagtatggc taaatttcat
    10321 ttaatataat acaaaaacac cgtccataag taagtggctt tcctcttgat aaaagaggca
    10381 ttcactcaat atgtgacggt gttttatata tttacgttaa cactattgct gtggcttttg
    10441 acggcctttt aataaaattg ctgtcgcacc tacgataata ataaatagaa tcgcaccaaa
    10501 taatcccata tattttactg cgttaccgaa cacgataccg acagttaaaa agtctgtatc
    10561 tgagaatgtt gttgcagcac cacctaattc gcctaaaaat ggcaagaata ataatggtaa
    10621 aaacgtgatt aggataccat ttagagcggc gccagcaaca gcacctttaa taccgcctct
    10681 tgcattaccg aatacagcag ccgttgcacc taagaagaag tgtgcaacta cgccaggtaa
    10741 aatgacgacg ccaccaaata agaataagat aaacataccg atgacacctg taataaagct
    10801 gacaaagaat ccaattaata ctgcattttg tgcataaggg aacacaatag ggcagtctaa
    10861 tgcaggttta gaatttggta caagcttttc agaaattcct ttaaatgctg ggacgatttc
    10921 agctaagatt aaacgaacgc ccgttaaaat aataaataca ccagcagcaa atgtcacacc
    10981 ttgaattaaa gaaaagacaa taaagttttg accatcacta atagattcgt gtacataact
    11041 aacgcccgca aataagcatg cgatgaagta aagtaatgcc attgtaatcg agatactaat
    11101 tgtactttct cgtaagaaac ttaagccttt tggaaattta atctcttccg ttgatttaga
    11161 cttacctttg aataattgac ctacagcacc tgcggcaaag taactgattg agccaaaatg
    11221 acctaacgct acttggtcat tccctgtaat ttttcgcatc gtaggttgga gtaatgcagg
    11281 taatactgcc atgattaatc ctaatacgag tgcgccgata acaattgtta gccagccttt
    11341 aatatgactg actgttaaaa tgattgctaa aaacgcagcc atgtaaaatg tatgatgacc
    11401 tgttaaaaag atatatttta aattagtgaa gcgagcaatt aaaatattaa caatcatgcc
    11461 acagaccatg atgagtgcag ctgttgttcc aaaatctttt aaggctagtg agacgatagc
    11521 ttcgttgtta ggtacgatac cttgcacacc aaatgcgtgt tggaatattt tgccgaatgg
    11581 ttcaagagat cgaacgacga catcagcacc tgcacttaaa attaagaagc ctaatatcgt
    11641 tttaatggtt cctgaagtga tcgttgcggc aggttttttc tgaacgatta aacctataaa
    11701 ggcaatcagt gcaacaagaa tggctggttg acttaaaata tcgactataa aattaaggat
    11761 tgcttgcata ggtcgtacct cctttaaatc atgttaagtt gttgtaattt ttctgagagc
    11821 ttttgttgta attcagcttt atctaaaata ttatcaagaa ctaagacatc ccctaggcgt
    11881 tcggcatttt cagctaaatc tctaccacaa ataaacaagt cagccatctc tggacttgct
    11941 gtcataatgt cactatgttc aacttcgata tcagatggtg cattaagttg cctaagtgct
    12001 tcttgtgcgt tcatttctac cataaaacta cttcctaaac cgtggccaca tactactaaa
    12061 attttcatat taatcatgct cctttaaaat gtttttaatg tcttgtgcgt tttttgcagt
    12121 taatagttgc tggactgttt ggttatcgcc cagtacggtt gctaaatttt gtaatacaga
    12181 taagtgtgaa tgattgtcga tggcactcaa tacaaaaatg agagatgcgt agtgatcttc
    12241 atcacaaaat gccacatgtt gattcaactt taatagactt aaaccaactt gatgtacgtc
    12301 attgttcggt cttgcatgtg caattgcaat ttcaggtgcg ataacgatat aaggtccaag
    12361 ttcattaacg ctatcaatca ttgcttgaac atagccttgt tcaataattt gttcttgtag
    12421 taatggctga gaagctatag ttatagcttc agtccaatca tttacttgtt cttttacaat
    12481 gatgcgtgtt gttgacaaaa tgtctaatga cacgttatta aacctccttt gtcatagtta
    12541 aagcaatgtg ttgtttaatt ttaaaaatat tcccatctaa gaaatcttgt cgatataagt
    12601 cgttgcttaa gcattcgctt aactgtccca atgcctttaa atgtgcattg gggtggtccg
    12661 ttgctaatgt aattacaagg tgaacgggat cgttagcttt actaccaaag ataatccctt
    12721 cagtgaaata tgttagtgcg aaacctacac cattctgtac ataatcagta ccagcgtgaa
    12781 taagtgcaat atgtggacta atgaccatat atgacccgaa ttgttcaaat tgttttaaaa
    12841 ttgcagctgt ataatttgaa tagacaatgc catcattgat taaaggttgc acagccactg
    12901 caattgcgga ttcaattgat aatggttgtt catttataat gatgcgatgt tcaggcaata
    12961 aatctgcgag tgacttgcca tcagttgcca ttttcatgac tcgttgttct cttgagtcat
    13021 tgataatttg attcaatttt tgacgagatt gttgattgat aaatggatcg acatgaataa
    13081 ctggtacagc tgatatttca caaggtactg ttgaaatgac ataatcaatg ttatcttgca
    13141 ataatcgact ttcttccaat tgataaatgg aataggcatc ccaaatgtga aactcaggat
    13201 acagatgatt tagttttgat tttaaaagtt gcgacgtgcc tataccagaa ccacatagta
    13261 agacaacctt aatcattgat tgtttatgtg ttgcaacacg ctctatactt gatgcgaagt
    13321 gaattgtaat gtatgttaat tcatcttcgt tgaagcgaat agcagcatct tgttcaattg
    13381 ggctaatatg cttgctaacg gcttcaatga tttgaggata gcgacgcata acttcttgcc
    13441 tcaaaggatt aggttgtagc atatcgtatt taatacgatg tatagctggt ttgatatgtg
    13501 tgatcagact ggtatgcaac ttgttgtctt ttgacatatc aatgcctaat tcttggctaa
    13561 cacaagtgat caattcatgt atattttgcg ataaatcatg gtattcaaag gtaattgaag
    13621 atgctgtatg ttcggtcatt ttagagccta gtaaatgtaa cgtgataaag ataatttcag
    13681 actctggaaa tgtgacatta caactgcgtt ctaagttttc tatcattttt gaagcaatag
    13741 catactgatt agtatgtcgc catttatcaa tttcattgat aggtatatcg aacgaaaaat
    13801 tttcatttaa acgctgaatg gcaatgagta tatgatagat taagccatcg atagccgact
    13861 gaactaaatg ataattttca ctatttaatg tcttaataat ggcacggcga accaatgcga
    13921 ttgattctga attaaagata tccgcctcta taaaaggtgc agcttgtttc atatattgat
    13981 gtataaagtg tgcatacgct ttacgataat gatcttcctc accaataaca ttgaatcctt
    14041 tattgtggac ataatttaac tttaaatggt attgatctag ttgggcttga atcattttaa
    14101 tatcatctgc aattgtccga cgcgaaacat taacatcttg cgcaagttgc tttgttgaaa
    14161 caggatcggt tgtttcgaat aactttaaag cgatatgtgt gagtcgttca tcttttgaaa
    14221 aatgaatttg atttgttaga ttgtgctcta attcatttaa tagtgtcgcg tgagctgttg
    14281 ttactttgat gcctgcagct ttattacggc tgacttggta atgataagtt tcagcatatt
    14341 gctcaatata tgctatatca tattgaatgg tacgaggtga tacaccaagt tgattagcaa
    14401 tggtattgat tggaataaac gtttgctcat gaattaaaag atacaaaatt tcgatttgtc
    14461 tataacttaa caacgtaata tcctcctatt tgtaattgta agcgatttct taaaaacgta
    14521 gatatgcaat ctctttcata ttttaatccg aaaaattgca tatcaaaatg tttatggcgc
    14581 aagattttat aggaactttt aaaataaatt agatattcat gttgacaatt taaaaatgtc
    14641 gcagtatatt tagttagaca tctaacgaaa tggtggtgca ataaatggaa ttcacttatt
    14701 cgtatttatt tagaatgatt agtcatgaga tgaaacaaaa ggctgatcaa aagttagagc
    14761 aatttgatat tacaaatgag caaggtcata cgttaggtta tctttatgca catcaacaag
    14821 atggactgac acaaaatgat attgctaaag cattacaacg aacaggtcca actgtcagta
    14881 atttattaag gaaccttgaa cgtaaaaagc tgatctatcg ctatgtcgat gcacaagata
    14941 cgagaagaaa gaatatagga ctgactacct ctgggattaa acttgtagaa gcattcactt
    15001 cgatatttga tgaaatggag caaacactcg tatcgcagtt atctgaagaa gaaaatgaac
    15061 aaatgaaagc aaacttaact aaaatgttat ctagtttaca ataaatggta agtgtgactg
    15121 gtagaaatca gtcactttat ctttaatatt atagttagat atctaattgt tagtaagcta
    15181 attattggaa aagacaagga gtattgaaca atgaaagacg aacaattata ttattttgag
    15241 aaatcgccag tatttaaagc gatgatgcat ttctcattgc caatgatgat agggacttta
    15301 ttaagcgtta tttatggcat attaaatatt tactttatag gatttttaga agatagccac
    15361 atgatttctg ctatctctct aacactgcca gtatttgcta tcttaatggg gttgggtaat
    15421 ttatttggcg ttggtgcagg aacttatatt tcacgtttat taggtgcgaa agactatagt
    15481 aagagtaaat ttgtaagtag tttctctatt tatggtggta ttgcactagg acttatcgtg
    15541 attttagtta ctttaccatt cagtgatcaa atcgcagcaa ttttaggagc gagaggtgaa
    15601 acgttagctt taacaagtaa ttatttgaaa gtaatgtttt taagtgcacc ttttgtaatt
    15661 ttgttcttca tattagaaca atttgcacgt gcaattgggg caccaatgat ttctatgatt
    15721 ggtatgttag ctagtgtagg cttaaatatt attttagatc caattttaat ttttggtttt
    15781 gatttaaacg ttgttggtgc agctttgggt actgcaatca gtaatgttgc tgctgctctg
    15841 ttctttatca tttattttat gaaaaatagt gacgttgtgt cagttaatat taaacttgcg
    15901 aaacctaata aagaaatgct ttctgaaatc tttaaaatag gtattcctgc atttttaatg
    15961 agtatcttaa tgggattcac aggattagtt ttaaatttat ttttagcaca ttatggaaac
    16021 ttcgcgattg caagttatgg tatctcattt agacttgtgc aatttccaga acttattatc
    16081 atgggattat gtgaaggtgt tgtaccacta attgcatata actttatggc aaataaaggc
    16141 cgtatgaaag acgttatcaa agcagttatc atgtctatcg gcgttatctt tgttgtatgt
    16201 atgattgctg tatttacaat tggacatcat atggtcggac tatttactac tgatcaagcc
    16261 attgttgaga tggcgacatt tattttgaaa gtaacaatgg catcattatt attaaatggt
    16321 ataggtttct tgtttactgg tatgcttcaa gcgactgggc aaggtcgtgg tgctacaatt
    16381 atggccattt tacaaggtgc gattatcatt ccagtattat ttattatgaa tgctttgttt
    16441 ggactaacag gtgtcatttg gtcattatta attgctgagt cactttgtgc tttagcagca
    16501 atgttaatcg tttatttatt acgtgatcgt ttgacagttg atacatctga attaatagaa
    16561 ggttaaatat ttcgtacact tctggctgag tatatttcgg tcggaagggt atttttcgaa
    16621 aaaaataaat atatgatacg attatgaaaa aataaagtga gattggcata tgtacaaatc
    16681 taaaatactg ttgaaatata tttttagtga agaatcagaa gttaaagatt taactgaaga
    16741 aaaatataat caagattacg aagcattaac atttagcttt aaagaggaaa catatcaaag
    16801 taggttagct aagaaaacac cgactaaagc agggtatttt gttacatgtt ggacgaaaga
    16861 cgagaacaat tgtaatcaac catactcgaa agaggcattt gcagattact taatgattat
    16921 agttattgat gaagaattaa gcggttattt tttatttcct agggaattat tggtagaaaa
    16981 aggtatctta actacatttg aacataaagg taagatggct tttagagttt atcctaagtg
    17041 gtgtaatcaa ttgaataaaa cagcagggca aacacaaaag tggcaatgta aatatttttt
    17101 tgaatactaa taaaaagtca tatggttgaa cttataagag atgaatatat catctttcga
    17161 taagataacc atatgacttt attttttatc atttaatgat gaacggtttc ttgtcctact
    17221 ttattccaag tgaggataaa gctcaacatt gcaaacacac tgattgctgt taataaaata
    17281 aaaccgacat cccatccgaa tttatcaact acagcaccta agacgatgtt ggccattaca
    17341 gcaccaaaca gataaccaaa taatcctgtt aatccagctg ctgtgccagc tgctttttta
    17401 ggtacataat ctaatgcttg taaaccaatt aacataactg gtccatatat taagaaacca
    17461 atggcaatta atgagacatg gtctaaccaa gcattgcctg gaggatttaa ccaataaatt
    17521 aatacaaata ctgtgacacc taacataaag aagaaacctg caggtccacg acgacctttg
    17581 aataatttat cagaaatgta accacataat aatgtaccag gaattccagc ccattcgtat
    17641 aagaagtatg cccaacctga tgcttttaag tcgaaatgtt tttcttcact taagtagact
    17701 ggcgcccaat caagtacacc ataacgcacg aaataaacaa atatatttgc aaaggcaatt
    17761 gcccataccc atttattgtt cagtacatat ttaaataaaa tttcttttgt agttaattct
    17821 gtttctaatg ttttcttatc gcttgtagca aagtcgtttt tataaatttc gattggaggt
    17881 aaaccttgag attgaggtgt gtctctaatc aatacgtatg aaattgcggc aatgataagt
    17941 gctaagagtg cagggtaaat gaatacacct tcgaaacctt ttaaataacc aaagttgata
    18001 aatgctgttg ttgtaatacc ccaagcagca ataggtgcca taatacctcc accaacatta
    18061 tgcgcaacgt tccaaagggc agtcttactt ccgcgttcac ttacactaaa ccagtgaacg
    18121 agaacacggc ctgaaggtgg ccagcccata ccttgaaacc atccatttaa gaataatagg
    18181 acaaacataa taccgatacc tgatgtaaag aacggtacaa atcccattaa caaattgacg
    18241 atagcagtga gtgctaatcc aagaactaag aatatccgag cattgctccg atcacttaca
    18301 gtacccataa agaacttact aaatccatat gcgatggaaa cagcagaaag tgcaaaacct
    18361 agttccgctt ttgtaaaacc ttgctcttgc aatgctggca tcgctaatga aaagttttta
    18421 cgtaataaat agtacccagc gtaaccgatg aaaataccaa gaaatacttg gagacgtaat
    18481 cgtttatagg tatcatctat ctgattttct ggcaaagact taatatgctt tgcaggttta
    18541 agaaaattca taaaatcctc cttaatatgt atttatatgc attttgtgcg ataaacttat
    18601 attagacatg tatgaaagcg ttgtcaatat acttttgtga aatcttgcat atttagttat
    18661 tgaagtattt tataaacatt tagaaaaaat aaattgctac acataaattt ataattatgc
    18721 aattattatg tcgttataca tactagctgt gtatctacga atgagtacaa acatattttt
    18781 atttgcagaa aaggggtaaa tggcatataa ctatcttttt tatgtaagtt ggtataaaat
    18841 ttatactaat aggagggata gtatgaatat agtagggcat catcacatat ccatgtatac
    18901 aaaagatgca aaacgtaata aggattttta cacaaatgtc cttggattac gattagttga
    18961 aaagtctgtt aatcaagaca atccttcaat gtatcatttg ttttatgggg acgaagtagg
    19021 tacagccgga acaattttaa gcttttttga aattccccat gcgggtcata agcagccagg
    19081 tactgaaacg atttatcgat tttctttatt agtaccaaat caagcggcac ttcattattt
    19141 tgaaaaacgc cttgataata atggtattac gtctgaacgt ttgtactatc ttggacaaga
    19201 aggtgttgtc tttaaagatg aagacgactt agaaatcata ttgcttgtta atgatagttt
    19261 tgaagtacca catcaatggc aacataacgc ttatagtgaa atacctcaag catatcaaat
    19321 tttaggaata gggccagtcg aattaagagt tagaaatgca gcgcgtacgg tagaattttt
    19381 ggaaaatgtc ttaggttatc gcaaaagaga taataaatca ttcgatgtgc tgacattagc
    19441 accacaaggt ttatattcgg attttgtagt tattgagcaa cagggacaac gtgaaagacc
    19501 tggacgaggt tatatccatc atattgcaat taatacacca caaatgagtg acttagatgc
    19561 aatttacaag aaattacaac aacaaacaca aagtaattca ggtataattg atcgctattt
    19621 ctttaaatca ttatactatc gccataattc aattatgtat gaatttgcga ctgaagcgcc
    19681 tggatttact attgatacac ctcttgaaca attaggaagt caattgaact tgcctgactt
    19741 tttagaagca gaacgtgaac aaattgaaag taagttacac gaaatataaa ggagaatgtt
    19801 taatggccaa attagaaatg aataaaaata cgcctcttga gtttggtttg tattccttag
    19861 gtgatcattt attgaatcca tttaaaggtg aaaaagttag ttatgagcaa cgtattaatg
    19921 aaattattga agcaagtaaa ttagcagatg aaacaggtat tgatgttttt gcagttggtg
    19981 aaagtcacca ggagcatttt acaacacagg cacatacggt tgtgttaggt gcaattgccc
    20041 aagcgacaaa gcatattaaa gtttcaagtt cttcaacgat tattagtgca acagatcctg
    20101 taagagtatt tgaagacttc gcgacattag atttgatttc tcatggtaga gccgaaattg
    20161 tagctggcag agcatcaaga acaggtattt ttgacttgtt tggctatgat ttaaaagact
    20221 atgatgaatt gtttgaagaa aaattaggtt tacttttaga gttaaataaa actgagcgta
    20281 ttacttggtc tggaaaatat cgtccagaac ttagaaatat gaaaatattc ccaagaccaa
    20341 tcgataatat attgccaata tggcgtgctg ttggtggtcc acctgcaagt gctattaaag
    20401 cgggaaaaca aggtgtgcca atgatgatta caacccttgg tggcccagca atgaacttta
    20461 aaggttctat agatgcttat cgtcaagcag caactgaagc aggtttcgat gcttcgcata
    20521 agtctttacc agtaagtaca gcgagtctgt tttatacagc tgaaacaact caggatgcta
    20581 tgagagaatt ttatccacat ttgaatacag ggatgtcatt tattcgtggt gttggttatc
    20641 cgaaacagca atttgctaat tcgtcagatt atcgagaagc gctaatggtt ggaagcccgc
    20701 aacaaattat tgaaaagata ttgtatcaac acgagttgta tggtcatcaa cgttttatgg
    20761 cacagcttga ttttggcggt gtgccatttg aaaatgttat gaagaatatt gagttaattg
    20821 gcaacgaaat tataccggcg attaaaaagc atttatcaaa ataggagggg cgtcatcatg
    20881 aatattgtat tattgtcagg ttccacagta ggttctaaaa cgagaattgc tatggatgat
    20941 ttaaaaaatg aactagaagt catcaatgag ggacatcaaa tagagttgat ggatttacga
    21001 gaacttgaat tagaatttag cgttggaaag aattatctag atactacagg agatgtatat
    21061 aaattaacga cgtcgttaat gcaggctgat gtgattttta ttggttttcc aatttttcaa
    21121 gcttccatcc ctggtgcttt gaaaaatgtg tttgatctac ttccagtcaa tgcttttcgt
    21181 gacaaggtaa taggacttgt agcgacagca ggttctagta aacattattt aattcctgaa
    21241 atgcatttaa aaccaatatt gagttacatg aaagcacata cgatgcaaac gtatgtattt
    21301 attgaagaga aagatttttc aaatcaacaa attgtcaatg atgatgttgt atttcggtta
    21361 aaagcgttgg cacaattcac aatgcgaact gccaaagtac aacaacaagt gtttgaagaa
    21421 gaaaacaacc aatacgactt ttaaagtata aaaataagac gctcggcaca ctaaatttgt
    21481 atgtgtttga gcgtctttta tattaactat atagccagtg aacgacgata aaggcaagtg
    21541 atgacaagca tattgaggta ataatgattg tcataagcgg tttaagtgca cgatttttaa
    21601 gatctttaaa tgcaacattt aaccctaaag caaccatggc cattaataag caaattgttg
    21661 atatagtatt taaaatattt aacaatactg atggaatagt tacatatgta ttcactaagg
    21721 ccataatgac aaatccaatt aaaaagtatg gaatgcttat tcgacccttg ctagatgatt
    21781 ctgatgaacg gaaacgcata attaaaataa gtacgatggt taatggaatc agtaagaata
    21841 ctctaccaag tttaccaaga agtgcaattt taagtgcatc actaccacca aagccaccag
    21901 ctaagacaac gtgcgcaatt tcatgaagac taacaccaga ccaagcgcca taaacatttg
    21961 tcgtcattga aaagatagcg tagatagctg tatatataag tgagaatatc gtaccaatca
    22021 atgcgatgat accgatacta atagctgtat ccttttcacg tgatttgaat attggagcga
    22081 ctgcggcaat agcggcagca ccacaaacgc ctgtgccgac acctagtaat aatgcgatgt
    22141 ttttgtcacc atgcaacagt ttgttgacaa agagcatcat tacaatactg aaaataacga
    22201 cacctacatc gatggctaat agtttactac cttgaccgat aatatcgaat atattgagtt
    22261 taagtccata taggatgatt gcaaatctta ataaatattt agatgaaaac gtaatacctg
    22321 agctatattg ttcaggatat cctctaaagt gacgatatag aatagcgatt aatatcgcga
    22381 tagttaatgc gccaacctta tctaagattg gcaatttagc tgctaaaaag ctaaataatg
    22441 cgactataaa tgttaatgat agtccaatca taaaatgctt atttttcaat gatgccatga
    22501 gcagtgcctc ctttaatagc attttagcac tgttttgtcg tatttttaaa tataaatttg
    22561 gaatggttaa taaagtagtg attaaattaa gttgtgtgat aggtatcaag gacatcaacc
    22621 aaagtaatag gcactacaac gcttattggc ggggccccaa caaagaagct gacgaaaagt
    22681 cagcttacaa taatgtgcaa gttggggatg ggccccaaca tagagaaatt gggtccgtaa
    22741 tttctacaga caatgcaagt tggcggggcc ccaacaaatt taagatacaa tgcgtaatat
    22801 taatatgtta ttattatgat aatttacaga attatatgaa aaatgaatga ggatgtgatg
    22861 gtatgtttgg aatgaaagtg aatgaacaaa taacattaaa aattttagaa gctcatgaca
    22921 cagaagcgct tttcaattta gtcaatcgtt caagaaattc acttagggaa tggttacctt
    22981 gggtagatgc aactgagcaa ccatcagata cgcgtgcatt tattaaaaga ggacttttgc
    23041 aatttgctga tggtaatgga tttcagtgtg gcatttggta tgaaggaacg ctagttggtg
    23101 tcatcggttt acatgaaatt aatcacatgc acagaaaaac ttcattaggg tactatttag
    23161 ataaacaatt tgagggtcat gggattatga cacaagcagt tgaggcattg ataaagtatt
    23221 gtttcgaaga gcttgactta aaccgaattg agattagtgc cgcagttaat aatgaaaaaa
    23281 gccgagctat tcctgaaagg ctgggattta ctagagaagg tatgttacgt gacaatgaat
    23341 tactaaatgg tatttattca tcgagttaca tctatagttt attaaaatca gaatacgacc
    23401 aaaaatgaca aattagactt acaaaagagt gatgacattt aaaatggcat cgctctttta
    23461 tttaattttt gaaaataaaa ggttgttgac agtattattt tataacaata taatgatttt
    23521 gataattatt atcaactaga taatgttcat gggaggatgc cttaaaatca tcattttaag
    23581 agccacgtat tatcttggca tttagggagt gtgagatagt acgaatgagg gcatgtctat
    23641 ggggataata gaatttctat aatgaggtgt caaaatgaaa aagttaacaa cgctattatt
    23701 agcatcaacg ttattaattg ctgcatgtgg gaacgacgat agtaagaagg atgattcaaa
    23761 gacatcgaaa aaagatgatg gtgttaaagc agaattaaaa caagcaacaa aagcatatga
    23821 taaatatact gatgaacagt taaatgaatt tttaaaaggt acagaaaaat ttgttaaagc
    23881 gattgaaaat aatgatatgg cccaagcaaa agcgttatat ccaaaagttc gtatgtatta
    23941 tgaacgctct gaaccagttg cagaagcatt tggagattta gatcctaaaa ttgatgcacg
    24001 tcttgcagat atgaaagaag agaaaaagga aaaagaatgg tcaggatatc ataagattga
    24061 aaaagcatta tacgaagata agaaaattga tgatgtgact aaaaaagatg cacaacaatt
    24121 attgaaagat gcaaaagaat tgcatgccaa agctgataca ttagatatca caccaaaatt
    24181 aatgttacaa ggttctgttg acctattaaa tgaagttgca acttctaaaa tcacaggtga
    24241 agaagaaatt tattcacata cagatttata tgattttaaa gcgaacgttg aaggcgcaca
    24301 aaaaatttat gacttattta aacctatttt agagaaaaaa gataaaaaat taagtgatga
    24361 tattcaaatg aacttcgata aagtgaatca attattggat aaatataaag ataacaacgg
    24421 cggttatgag tcatttgaaa aagtatcgaa gaaagaccgt aaagcatttg cggatgctgt
    24481 taatgcatta ggagagccac taagtaaaat ggctgtgatt actgaatgac aaattatgaa
    24541 caagttaacg atagtacgca attttcaaga cgtacatttt tgaaaatgtt aggtattggc
    24601 ggtgccggtg ttgcaattgg cgcaagtggt gttggtagca tgtggtcttt caaatcaatg
    24661 ttcaatacac cagaagatcc ggaaaaagat gcgtatgaat tttatggtaa agtgcaacca
    24721 ggcattacca cacccacgca aaaaacatgc aatttcgttg cgttagattt gaagtcaaaa
    24781 gatagagatg caattaaggc aatgtttaaa aagtggacgg ttatggctga tcgtatgatg
    24841 gatggtgata cagttggcaa gacgagtaac aatcctttaa tgccaccagt agataccggt
    24901 gaatcgatag gattaggtgc aagcaagtta acgattacct ttgggattag taagtctttg
    24961 atgaagaaaa ttgggttatc tagtaaaatt cccgatgcct ttaaagattt accgcatttt
    25021 ccgaatgatc agttaataga cgattacagc gatggtgata ttatgattca agcatgctca
    25081 aatgattcgc aagtatcctt tcatgcggtt cataatttag ttcgtccatt tcgagatatt
    25141 gttaaggtac gttgggcgca atctggtttt atctctgcta aaggtaagga aacacctaga
    25201 aatttaatgg catttaaaga tggaacaatt aatcctagaa agagtaatca acttaaagat
    25261 tatgtgttta ttgatgacgg atgggcgaaa catggaactt attgtgttgt cagacgtatt
    25321 caaatacaca ttgaaacgtg ggatcgtact gcactggaag aacaagaggc tacatttggt
    25381 cggaaacgac atagtggtgc gccgttaaca ggtgggaaag agtttgatga aattgactta
    25441 aaagcgaaag atagtcatgg cgagtatatt attgataaag atgcccatac gagactagcg
    25501 aaagaagcaa atacgtcaat tttacgtaga gcctttaatt atgttgatgg tacggatgac
    25561 cgcacaggta acttcgaaac aggcttgttg ttcattgctt ttcaaaaagc gacaaaacaa
    25621 tttatcgata tacaaaataa tttaggtagt aatgataaat taaatgaata tattacacat
    25681 agaggttctg cttcattttt agtattacca ggtgttagta agggaggata ccttggtgaa
    25741 acattatttg actaaatttg tagcaatgct aataactgct gctatggtgt gtagctttgg
    25801 gttactgaaa agtcaggcag cagaacaaca aagtattagt gatgtatata gtgtgataac
    25861 ggatgcgaaa tctgcacttt ctaataattc gatatcgaat gacaataagc agaaagcaat
    25921 tgagcaagtg gtaagtgcag ttaagaaatt atcgcttgaa gataatagtg aaggtaatgc
    25981 tgtcaaatca gatgtgagaa agcttgaaga tgcaaaagcg aatgataatc aaaaagatac
    26041 actttcgcaa ttaacgaagt cattaattgc ttatgaagag aaattggcta gtaaagatgc
    26101 gggttctaaa attaaactat tgcaacagca agtcgatgcc aaagatgctg cgatgacaaa
    26161 agcgattaaa gataaaaata aagcggaatt agaatctttg aacaatagtt tgaatcagat
    26221 ttggacaagt aatgaaacag tgattcgcaa ttatgacgca aatcaatatg gacaaattga
    26281 agtcgcatta ttacaactta gaattgcaat tcataagtca ccattagata cggcaaaagt
    26341 gtcacatgct tggacaactt ttaaatcaaa tattgatcat gtcgataaaa aagtgatacg
    26401 tctgcaaatg atcaatacca tgtatcacaa ttaaatgatg cgttagagaa ggcgattaaa
    26461 gctatcgacg acaatcaatt gtcggatgct gatgctgcgc ttacacattt tatagaaatt
    26521 tggccgtatg ttgaaggtca aattcaaact aaagacgggg ctttgtatac gaaaattgaa
    26581 gataaaatac catattatca aagtgtatta gacgaacata ataaatcgca tgtcaaagat
    26641 ggtttagtag atataaacaa tcaaattaaa gaggttgttg gccatagtta tagctttgtc
    26701 gatgtgatga ttatcttttt acgtgaagga ctagaagtgt tattaattgt aatgacattg
    26761 actaccatga cgcgtaaagt caaagataat aaggggactg caagtgtgat tggtggtgca
    26821 attgccggac ttgtactgag tattatctta gcaattacgt ttgtagaaac tttagggaat
    26881 agtggcattc ttcgtgaaag tatggaagcg ggattaggta tcgttgcggt catattaatg
    26941 tttatcgttg gtgtttggat gcacaaacgt tcaaatgcga aacgctggaa tgatatgatt
    27001 aaaaatatgt atgccaatgc gattagtaat ggtaatttgg tattgttagc gacgattggt
    27061 ttaatatctg tgttgcgtga aggtgttgag gttatcattt tctatatggg gatgataggt
    27121 gagctagcga caaaagattt tattattggt attgccttgg ccatagtgat attaattgtc
    27181 tttgcggtat tatttagatt tatcgtcaga ttgataccta tattctatat atttagagta
    27241 ttgtcgatct ttatttttat tatgggattc aaaatgcttg gcgtaagtat tcaaaagtta
    27301 caattattag gtgcgatgcc aagacatgtt attgaaggat tcccaacgat taactggttg
    27361 ggcttctatc caagttatga accattgata gcacaagctg cttatattat ggtagttgct
    27421 atcttaatct ttaaatttaa aaaataaaaa acaggccgag tgcctgtttt tttgttgctg
    27481 tattgcggat tatcgtagca ttgttgagat gtggatatcg ctgcatggtt ggttctcgtt
    27541 gtattgcaga tatcacggtt taatccgatt ctgtattcta attaacgcaa gtgcagtatt
    27601 gatataatct gaggtgctac attgttcttt tattggctat tgtgatgttg ctgcattgtg
    27661 agtatcacag cattaattcg tataaggtta atgtgttggc ggtttgcctc ggcatgtgaa
    27721 cttaacaatg aacatactga actcaaagag caatatgagt ggcaatgtaa gtaatatatt
    27781 taatgttaaa tcgggtggtg caatgatact tgccagcaca aagcaagcga aataaatata
    27841 ttttcgataa tgcttcaatg atgtgatatc tataagaccg aattttgcaa gtcctatgaa
    27901 taatattggc aattggaata aaatgccaaa agtgaaaagc caacggataa gttcaactaa
    27961 atacgcttta aagccaatga caggcgaaat gtttaaagtt aatgataatt ttaacgcgaa
    28021 ttgaatgatt attggaaagc caacataaaa tgcaaaagcg acgccagcac agaataataa
    28081 cacgctgaaa aaactata
//
LOCUS       DAOBBH010000015        28067 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.015, whole genome shotgun sequence.
ACCESSION   DAOBBH010000015 DAOBBH010000000
VERSION     DAOBBH010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 28067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28067
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.015"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            96..1085
                     /gene="ccpA"
                     /locus_tag="P6L01_000442"
     CDS             96..1085
                     /gene="ccpA"
                     /locus_tag="P6L01_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDP3029537.1"
                     /translation="MTVTIYDVAREARVSMATVSRVVNGNQNVKAETKNKVNEVIKRL
                     NYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDP
                     EKEKEIFNNLLSKQVDGIIFLGGTITEEMKELINQSSVPVVVSGTNGKDAHIASVNID
                     FTEAAKEITGELIEKGAKSFALVGGEHSKKAQEDVLEGLTEVLNKNGLQLGDTLNCSG
                     AESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRL
                     VEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK"
     gene            complement(1183..2352)
                     /locus_tag="P6L01_000443"
     CDS             complement(1183..2352)
                     /locus_tag="P6L01_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HDP3029538.1"
                     /translation="MQQHSSKTAYVYSDKLLQYRFHDQHPFNQMRLKLTTELLLNANL
                     LSPEQIVQPRIATGDELMLIHKYDYVEAIKHASHGIISEDEAKKYGLNDEENGQFKHM
                     HRHSATIVGGALTLADLIMSGKVLNGCHLGGGLHHAQPGRASGFCIYNDIAITAQYLA
                     KEYNQRVLIIDTDAHHGDGTQWSFYADNHVTTYSIHETGKFLFPGSGHYTERGEDIGY
                     GHTVNVPLEPYTEDASFLECFKLTVEPVVKSFKPDIILSVNGVDIHYRDPLTHLNCTL
                     HSLYEIPYFVKYLADSYTNGKVIMFGGGGYNIWRVVPRAWSHVFLSLIDQPIQSGYLP
                     LEWINKWKHYSSELLPKRWEDRLNDYTYVPRTKEISEKNKKLALHIASWYESTRQ"
     gene            complement(2377..3009)
                     /locus_tag="P6L01_000444"
     CDS             complement(2377..3009)
                     /locus_tag="P6L01_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDP3029539.1"
                     /translation="MNHLKTYQSEDYYIHDKQFVIEGPLTYEDLKALTFDAHLTAFRD
                     AEDQYEALLEITTLPEGRIYVARQDELIVGYVTFHYPDEIERWSTGNLPYLIELGAIE
                     VSINFRQLHLAEKLIQLSLSTPEFEDYIVITTEYYWHWDLKNSKLDVFDYKKLMQRLM
                     ATGGLEIFATDDPEITSHPANCLMARIGKNITLEQQQAFDDIRYMNRFFF"
     gene            3178..4884
                     /gene="acsA"
                     /locus_tag="P6L01_000445"
     CDS             3178..4884
                     /gene="acsA"
                     /locus_tag="P6L01_000445"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HDP3029540.1"
                     /translation="MKVEVYKGAQGKHNLKDYEETYNTFDWKDVEQAFSWSETGKMNM
                     AYECIDRHVDQGLGDKIALNYKDEYRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRV
                     FIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRV
                     PVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQ
                     PKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCI
                     AGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPL
                     NPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDA
                     GNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQ
                     GRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEP
                     TDELKEEIRLFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLST
                     ME"
     gene            5324..6991
                     /locus_tag="P6L01_000446"
     CDS             5324..6991
                     /locus_tag="P6L01_000446"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDP3029541.1"
                     /translation="MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDI
                     NKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTF
                     GIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRR
                     IEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDK
                     ISRITIGYTRDRKPVTVADLKVQGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIA
                     HGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKAREAGFDLAAVVVVATIRAL
                     KMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYV
                     KSWAKENNVRIALTEVWEKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKI
                     VTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEIT
                     IRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF"
     gene            complement(7236..7325)
                     /locus_tag="P6L01_000447"
     CDS             complement(7236..7325)
                     /locus_tag="P6L01_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029542.1"
                     /translation="MLLTTPIRLYLIDNNYQYQLLVLIVAHPN"
     gene            7324..9999
                     /gene="harA"
                     /locus_tag="P6L01_000448"
     CDS             7324..9999
                     /gene="harA"
                     /locus_tag="P6L01_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haptoglobin-binding heme uptake protein HarA"
                     /protein_id="HDP3029543.1"
                     /translation="MNKHHPKLRSFYSIRKSTLGVASVIVSTLFLITSQHQAQAAENT
                     NTSDKISENQNNNATTTQQPKDTNQTQPATQPVITAKNYPAADESLKDAIKDPALENK
                     EHDIGPREQVNFQLLDKNNETQYYHFFSIKDPADVYYTKKKAEVELDINTASTWKKFE
                     VYENNQKLPVRLVSYSPVPEDHAYIRFPVSDGTQELKIVSSTQIDDGEETNYDYTKLV
                     FAKPIYNDPSLVKSDTNDAVVTNDQSSSDASNQTNTNTSNQNTSTTNNANNQPQATTN
                     MSQPAQPKSSANADQASSQPAHETNSNGNTNDKTNESSNQSDVNQQYPPADESLQDAI
                     KNPAIIDKEHTADNWRPIDFQMKNDKGERQFYHYASTVEPATVIFTKTGPVIELGLKT
                     ASTWKKFEVYEGDKKLPVELVSYDSDKDYAYIRFPVSNGTRDVKIVSSIEYGENIHED
                     YDYTLMAFAQPITNNPDDYVDEETYNLQKLLAPYHKAKTLERQVYELEKLQEKLPEKY
                     KAEYKKKLDQTRVELADQVKSAVTEFENVTPTNDQLTDLQEAHFVVFESEENSESVMD
                     GFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYIP
                     DKAVYNAIVKVVVANIGYEGQYHVRIINQDINTKDDDTSQNNTSEPLNVQTGQEGKVA
                     DTDVAENSSTATNPKDASDKADVIEPESDVVKDADNNIDKDVQHDVDHLSDMSDNNHF
                     DKYDLKEMDTQIAKDTDRNVDNSVGMSSNVDTDKDSNKNKDKVIQLAHIADKNNHTGK
                     AAKLDVVKQNYNNTDKVTDKKTTEHLPSDIHKTVDKTVKTKEKAGTPSKENKLSQSKM
                     LPKTGETTSSQSWWGLYALLGMLALFIPKFRKESK"
     gene            10130..10222
                     /locus_tag="P6L01_000449"
     CDS             10130..10222
                     /locus_tag="P6L01_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029544.1"
                     /translation="MKIILLLFLIFGCIVVVTLKSEHQLTLFSI"
     gene            complement(10202..11107)
                     /locus_tag="P6L01_000450"
     CDS             complement(10202..11107)
                     /locus_tag="P6L01_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDP3029545.1"
                     /translation="MTNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVI
                     AMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKT
                     SSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTDQSSGGSTITQQLVKNQVL
                     TNEKTYSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGRDYNGANISGIASASYSLF
                     GIPPKDLSIAQSAYLIGLLQSPYGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIED
                     QITEKEYNDALKYDIKSHLLNRKKR"
     gene            11489..12751
                     /locus_tag="P6L01_000451"
     CDS             11489..12751
                     /locus_tag="P6L01_000451"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDP3029546.1"
                     /translation="MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTAD
                     SLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEG
                     ISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLE
                     HGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYG
                     LTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGK
                     EEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAK
                     ELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNY
                     ALAPEDKIDGEFTIIRRGKKKYFMVNYQ"
     gene            complement(12845..14119)
                     /locus_tag="P6L01_000452"
     CDS             complement(12845..14119)
                     /locus_tag="P6L01_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDP3029547.1"
                     /translation="MSDFNHTDHSTTNHSQTPRYRRPKFPWFKTVIVALIAGIIGALL
                     VLGIGKVLNSTILNKDGSTVQTTNNKGGNQLDGQSKKFGTVHEMIKSVSPTIVGVINM
                     QKASSVDDLLKGKSSKPSEAGVGSGVIYQINNNSAYIVTNNHVIDGANEIRVQLHNKK
                     QVKAKLVGKDAVTDIAVLKIENTKGIKAIQFANSSKVQTGDSVFAMGNPLGLQFANSV
                     TSGIISASERTIDAETTGGNTKVSVLQTDAAINPGNSGGALVDINGNLVGINSMKIAA
                     TQVEGIGFAIPSNEVKVTIEQLVKHGKIDRPSIGIGLINLKDIPEEEREQLHTDREDG
                     IYVAKADSDIDLKKGDIITEIDGKKIKDDVDLRSYLYENKKPGESVTVTVIRDGKTKE
                     VKVKLKQQKEQPKRQSRSERQSPGQGDRDFFR"
     gene            14291..14908
                     /locus_tag="P6L01_000453"
     CDS             14291..14908
                     /locus_tag="P6L01_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDP3029548.1"
                     /translation="MYSVISKILNFILVKMSKSLYVIGKDNIPKDSKYVVTCTHESYN
                     EVIMLGMALYPNQIHYMAKKELFKNKWIGKFLTSLNAFPVDRENPGPSTLKRPINLLK
                     DNKTVGIFPTGSRTSQEGAPLKRGASTIAMLSKSPILPVAYVGPTKIHGLLTGQAYIN
                     IGKPIDINDLPKDLKRNERIDYITKEIETRTAKLQQELHEIVKSL"
     gene            15036..16502
                     /locus_tag="P6L01_000454"
     CDS             15036..16502
                     /locus_tag="P6L01_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HDP3029549.1"
                     /translation="MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMF
                     FTTVGTTILEQLGILFAIGVAIGMAKKNDGAVALAATLGYFLVTVVLSPMKLAPLLGM
                     KASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAF
                     YCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVF
                     WFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAIYHTAKTTQKKQV
                     YGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLFIAATFHWTAGFSFS
                     AGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDP
                     TVVKDNIAPGENDIKQSKYHQHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDQ
                     QKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL"
     gene            16609..17739
                     /locus_tag="P6L01_000455"
     CDS             16609..17739
                     /locus_tag="P6L01_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDP3029550.1"
                     /translation="MKKILFDVDGIFLSEERCFDVSALTVYELLMDKCYLGLHSHIDW
                     ETLTDNDIQDIRNRIFQKDKILNKLKSLGLNSNWDMLFIVFSIHLIDILKKLSHDEIE
                     AFMYQDEPVELKLQNISTNLADCFNLNEQLPLQFLDNVKVGKNNIYAALEEFATTELH
                     VSDATLFSLKGALWTLAQEVYQEWYLGSKLYEDVEKKIARTTFKTGYIYQEIILRPVD
                     EVKVLLNDLKGAGFELGIATGRPYTETVVPFENLGLLPYFEADFIATASDVLEAENMY
                     PQARPLGKPNPFSYIAALYGNNRDKYESYINKQDNIVNKDDVFIVGDSLADLLSAQKI
                     GATFIGTLTGLKGKDAAGELEAHHADYVINHLGELRGVLDNL"
     gene            complement(17700..17795)
                     /locus_tag="P6L01_000456"
     CDS             complement(17700..17795)
                     /locus_tag="P6L01_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029551.1"
                     /translation="MKPLVQSFTSSYAVKQQSNYKLSSTPLSSPK"
     gene            complement(17889..19493)
                     /locus_tag="P6L01_000457"
     CDS             complement(17889..19493)
                     /locus_tag="P6L01_000457"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDP3029552.1"
                     /translation="MKQFNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIP
                     SYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNININAATLKGILVINAPDGNTI
                     SATEHSLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAK
                     RAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAD
                     FFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKI
                     IVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINL
                     SQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINAL
                     MLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGPRIVRINNFSV
                     DLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPV
                     SNNIIDELKQVGEYNQIFTTELTVQS"
     gene            complement(19480..20637)
                     /locus_tag="P6L01_000458"
     CDS             complement(19480..20637)
                     /locus_tag="P6L01_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDP3029553.1"
                     /translation="MYYHQPLLLTPGPTPLPDAIMREIQAPMVGHRSKDFEDIAQQAF
                     QGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYY
                     KNVHIYDVTWGEAVDVKDFINYLSTLDVEVKAVFSQYCETSTTVLHPIHELGNAINQF
                     NSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKERFKE
                     VTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRN
                     ALRSALKALDLTLLVNDKDASPTVTAFKPNTNEVKIIKDELKNRFKITIAGGQGHLKG
                     QILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI"
     gene            complement(20751..21197)
                     /locus_tag="P6L01_000459"
     CDS             complement(20751..21197)
                     /locus_tag="P6L01_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDP3029554.1"
                     /translation="MHQHDFKVQTSWQGGRNNVGNVQGDILSENISIPASLGGVGIGT
                     NPDEMLVSAASSCYIISLAATLERAKFTDISIEQQSIGTACLNNGKFSMSKIVHHPHI
                     EIPSDQIAQLEKRLPKLITIADNNCMISNAVRNNVDIKIYPIIQAK"
     gene            21277..22020
                     /locus_tag="P6L01_000460"
     CDS             21277..22020
                     /locus_tag="P6L01_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDP3029555.1"
                     /translation="MTLNKLKDELQIVSHRGLPSDFPENTMVGYREVMGLNVAMLEID
                     VHLTKDQHFVVIHDETIDRTSDGKGRIADYTLSQLKSFDFGSYKDVAFKGERIPTLDE
                     VLSLCLKYDKKLLIELKSPNLYPEIECKLLAFLEEKKVDATQVVIQSFDIECIEKLNT
                     LGSIYELGVLCSKRNYWYKKPNFSRIAQIASYVNPNYALVARKFVDKAHHHQLQVMPY
                     TVNKLKTGEKLRQMGVDGLITDNPKLFIK"
     gene            complement(22236..22838)
                     /gene="rpsD"
                     /locus_tag="P6L01_000461"
     CDS             complement(22236..22838)
                     /gene="rpsD"
                     /locus_tag="P6L01_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDP3029556.1"
                     /translation="MARFRGSNWKKSRRLGISLSGTGKELEKRPYAPGQHGPNQRKKL
                     SEYGLQLREKQKLRYLYGMTERQFRNTFDIAGKKFGVHGENFMILLASRLDAVVYSLG
                     LARTRRQARQLVNHGHILVDGKRVDIPSYSVKPGQTISVREKSQKLNIIVESVEINNF
                     VPEYLNFDADSLTGTFVRLPERSELPAEINEQLIVEYYSR"
     gene            complement(23082..23546)
                     /locus_tag="P6L01_000462"
     CDS             complement(23082..23546)
                     /locus_tag="P6L01_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDP3029557.1"
                     /translation="MTTINPTNYTLLKKQAASLIEDEHHMIAILSNMSALLNDNLNQI
                     NWVGFYLLEQNELILGPFQGHPACVHIPIGKGVCGTAVSERRTQVVADVHQFEGHIAC
                     DANSKSEIVVPIFKDDKIIGVLDIDAPITDRFDDNDKEHLEAIVKIIEKQLA"
     gene            23683..25377
                     /gene="ezrA"
                     /locus_tag="P6L01_000463"
     CDS             23683..25377
                     /gene="ezrA"
                     /locus_tag="P6L01_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDP3029558.1"
                     /translation="MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETL
                     PFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNA
                     SQSEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANR
                     HQFGEAASLLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIP
                     ELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLE
                     LEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIE
                     YVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHV
                     TVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMK
                     NEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQY
                     GNRYRKDYSNVDKSLNEAERLFKNNRYKRAIEIAEQALESVEPGVTKHIEEEVIKQ"
     gene            complement(25451..25592)
                     /locus_tag="P6L01_000464"
                     /pseudo
     CDS             complement(25451..25592)
                     /locus_tag="P6L01_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500331.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25741..26889
                     /locus_tag="P6L01_000465"
     CDS             25741..26889
                     /locus_tag="P6L01_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDP3029559.1"
                     /translation="MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQA
                     NQLLQQAKAQINAMINSKTNYDVVFTSGATESNNLALKGIAYRKFDTAKEIITSVLEH
                     PSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELVSDKVGLVTCMYVNNVTGQIQ
                     PIPQMAKVIKNYPKAHFHVDAVQAFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNH
                     IQNVEPTVHGGGQEYGVRSGTVNLPNDIAMVKAMKIANENFEALNAFVTELNNDVRQF
                     LNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNE
                     VLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK"
     gene            26889..>28067
                     /gene="thiI"
                     /locus_tag="P6L01_000466"
     CDS             26889..>28067
                     /gene="thiI"
                     /locus_tag="P6L01_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDP3029560.1"
                     /translation="MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVK
                     GKRDRMYIELEDHADINEITYRLSKIFGIKSISPVLKVEKTIEAMSAAAIKFAQQFEE
                     NSTFKIDVKRADKNFPMDTYELQRELGGTVLKQIENVSVNVKRPDHEIRVEVRLDAIY
                     MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQ
                     AKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLV
                     HQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTF
                     ETSIQPFEDCCTIFTPKNPVTEPNFEKVVQYESVFDFEEMINRAVENIETLEITSDYK
                     T"
BASE COUNT         9792 a         4099 c         5121 g         9055 t
ORIGIN      
        1 aaaaatatgt tactgtaaat taaaatatgg taaactatga aaatgaaatg aaaacatgtt
       61 attataatga ataaaacgtt tacaaggagg aaattatgac agttactata tatgatgtag
      121 caagagaagc gcgtgtctct atggccacag tgtcgcgtgt tgttaatggg aaccaaaatg
      181 ttaaagcaga aactaaaaat aaagttaacg aagtcattaa gcgtttgaat tatcgtccaa
      241 atgctgttgc tagaggttta gctagtaaaa agacaacaac agtaggtgtg atcattccag
      301 atatatctaa tatctattat tcacaacttg ctcgtggact tgaagatatt gcaacaatgt
      361 ataaatatca ctcaattatt tcaaattcag ataacgatcc tgaaaaggaa aaagaaattt
      421 ttaataactt attaagtaaa caggttgatg gtattatttt ccttggtggt acaattactg
      481 aagaaatgaa agaattgata aatcaatcat ctgtacctgt agtagtatca ggaacaaatg
      541 gtaaggatgc acatatagca tcagttaata ttgattttac tgaagctgcg aaagaaatta
      601 cgggagaatt aattgaaaaa ggcgctaaat catttgcttt agtaggtgga gaacattcta
      661 aaaaagctca agaagatgtt ttagaaggtt taactgaagt gttaaataaa aatggccttc
      721 aattaggtga tacattgaat tgttctggtg ctgaaagtta taaagaaggc gtaaaagctt
      781 ttgctaaaat gaaaggcaat ttgccagatg ccattttatg tatcagcgac gaagaagcaa
      841 ttggtattat gcatagtgca atggatgctg gtattaaagt tccagaggaa ttacaaatta
      901 ttagtttcaa taatacacga ttagttgaga tggttagacc acaactttct agtgttattc
      961 aaccattata tgatatcggt gcagtaggga tgcgcttatt aacaaaatat atgaacgatg
     1021 aaaagataga agaaccaaat gtagttttac ctcacagaat tgaataccga ggaactacaa
     1081 aataaattca caaaattagg cattcatcta acgacccaaa ttatatgggt gttggaagaa
     1141 tgccttttat ttatctttta aaatcgttgc agattaggtt acttattgac gagtagattc
     1201 gtaccaactc gctatatgta aagctaattt tttatttttt tcactaattt cttttgtgcg
     1261 ggggacatag gtataatcat ttaaacgatc ttcccatctt ttaggtaata attcagatga
     1321 ataatgtttc catttattaa tccattctaa cggtaaataa ccactttgaa ttggttgatc
     1381 aattaaactt aagaatacat gactccatgc acgtggtacg actctccaaa tattgtagcc
     1441 tccgccacca aacataatta cctttccatt cgtataagaa tcagctaaat attttacaaa
     1501 atatggaatt tcatataatg aatgtaacgt acaatttaga tgagttagtg gatcacgata
     1561 atgtatatcg acaccattta cgcttagaat aatatcaggt ttaaaactct ttacgacagg
     1621 ctcaactgtt aatttaaaac actccaaaaa tgatgcatct tctgtatacg gttcaagtgg
     1681 gacatttaca gtgtgtccat agccgatatc ttcaccgcgt tcagtatagt gaccagagcc
     1741 tgggaaaaga aactttccgg tttcatggat agaataagta gtaacatggt tatcggcata
     1801 gaaactccat tgtgtaccat ctccatgatg tgcatcggta tctatgatta aaacgcgttg
     1861 attgtattct ttagctaagt attgtgcggt aattgcaata tcattgtata tacaaaaacc
     1921 acttgctcga ccaggttgag cgtgatgcaa accaccacct aagtgacaac catttaatac
     1981 tttgcctgac ataataagat ctgctaaagt taaagcgcct ccaacaattg tggcactatg
     2041 gcggtgcata tgcttaaatt gaccattctc ttcatcattt aatccatatt tcttagcctc
     2101 atcttcactg ataatgccat gtgaagcatg cttaatagct tcgacgtaat catatttatg
     2161 aattaacatt aattcgtcac ctgttgcaat tctaggttgt actatttgtt ctggagacaa
     2221 taaatttgca ttcaaaagta gctctgttgt taattttaaa cgcatttgat tgaagggatg
     2281 ttggtcatga aatcgatatt gtaataactt atctgaataa acatatgcag tttttgatga
     2341 atgttgttgc atataatccc tccgatattc caaaaattaa aagaaaaacc gattcatata
     2401 acgaatatca tcaaacgctt gttgctgttc taatgtaata tttttgccaa ttcttgccat
     2461 taaacaatta gctggatgac ttgttatttc tggatcatct gtagcgaata tttcaagtcc
     2521 accagttgcc attaaccgct gcattaattt tttatagtca aatacatcta actttgaatt
     2581 ttttaaatcc caatgccagt aatattcagt agttataacg atataatcct cgaattctgg
     2641 tgtagaaagg ctaagttgta ttagcttttc tgcaagatgt aattgcctaa aattgatgct
     2701 gacttcaatt gcccccaatt cgattaaata tggaaggtta cctgtagacc agcgctcaat
     2761 ttcatcagga tagtggaaag tgacataacc cacaatgagt tcatcttggc gagcaacata
     2821 aattctacct tctggtaatg ttgtaatttc taacaaagct tcatactgat cttcagcatc
     2881 tctaaatgcg gttaaatgcg catcgaaagt aagcgctttc aaatcttcgt atgttaaagg
     2941 accttcaata acaaattgct tgtcatgaat gtaataatct tcggattgat acgtctttaa
     3001 atgattcata ttttcaactc ctcaatcgac gtcgtgattg tattaaattc attatataga
     3061 aaatttacaa taattaatac tagaaaaagg ataaaagtaa aaattttgaa taattagaaa
     3121 tgttatgtat aatattgaga aagaaagcgt tttcacataa caaaggggga gtttcaaatg
     3181 aaagtcgaag tttataaagg agcgcaaggt aaacataacc ttaaagatta tgaagaaaca
     3241 tataatactt ttgattggaa agacgtagaa caagcatttt cttggagtga aactggaaaa
     3301 atgaacatgg catatgaatg tatagatcgc catgtagatc aaggattagg ggataaaata
     3361 gcgttaaatt acaaagatga gtacagaaaa gaatcgtata cttataaaga tatgcaacgg
     3421 ttatctaata aagcagcgaa tgttttgtct gaacatgcag aagttgacaa aggtgacaga
     3481 gtatttatat ttatgtcgcg tacacctgaa ctatattttg cgttgttagg tgttttaaaa
     3541 attggtgcaa ttgttgggcc gttatttgaa gcatttatgg aaaaggcagt tgcggataga
     3601 ttagagaaca gtgaagctaa agtgttaatt actaataagg cattgttacc tcgagtacct
     3661 gtagataaat taccgaactt gaaaaaaatt gttgtcgtag atgaggatgt agaagacaat
     3721 tacatagact tcattagttt gatggaaact gctagcgatg aatttgacat tgaatggtta
     3781 aagtcggatg atggtttgat tttacattat acatcaggtt ctactgggca acctaaaggt
     3841 gtattgcatg ttcaacaagc aatgttagtg cactatattt ctggaaaata tgtattagat
     3901 ttacaagaag atgatgttta ttggtgtaca gcagatccag gttgggttac aggaacatct
     3961 tatggtattt ttgcaccatg gttaaatggc gctacaaatt gtatagctgg tggtcgcttt
     4021 tcaccagaac agtggtatag tatgattgaa gattttaaag tgacgatttg gtatacggca
     4081 ccaacagctt taagaatgtt aatgagtgct ggtgacgata ttgttgagaa atatgacttg
     4141 tcatcgttac gttcgattct atcagtaggt gagcctttaa atcctgaagt tataaaatgg
     4201 gcgaaaaaag tatacggttt aacggtgtta gatacttggt ggatgacaga aacaggtgga
     4261 catatgattg ttaactatcc aacgatggac gtcaagcttg gctcaatggg caaaccatta
     4321 cctggtattc aagctgcaat tatcgatgat gcaggtaatg aattaccacc aaatcgaatg
     4381 ggcaaccttg ctataaaaaa aggctggcca tcaatgatgt atcgtatctg gaagaatcca
     4441 gaaaaatata aatcatattt tattggagac tggtatgtat ctggtgattc ggcatataaa
     4501 gatgaagatg gttacttctg gttccaagga cgtgttgatg atgtaattat gacagctggt
     4561 gaacgagttg gaccatttga ggttgagtct aaattggttg aacacgaagc agttgccgaa
     4621 gcaggaatta ttggtaaacc tgatccggtt cgcggtgaaa taattaaggc gtttgttgca
     4681 ctgagaaaag gatatgaacc aacagacgaa ttaaaagaag aaattcgttt atttgttaaa
     4741 gaaggtttgt cggcacatgc agcaccacgt gaaatcgaat ttaaagataa attacctaaa
     4801 acacggtcag gtaaaattat gagacgtgta ttaaaagctt gggaattaaa tttagatgct
     4861 ggggatttaa gtacaatgga ataatgacat gaatgttatt gaagattttt ttcgaagaat
     4921 aaagggtgac aacatatttc atgtcaatgt ttaaataatc gtttacttta cgataagcaa
     4981 tataaagagc tgttaacttg tgtcatatca tttcgtagaa agcatttgaa aatgatgaca
     5041 taacaattat ggcatatctt tatattgctt tttattttta atatgatctt tggaagatga
     5101 ttattttaaa taatagaaaa atatagttat caatagtatc aagcgctaaa agttttataa
     5161 tacaaaactt taataagtga atttattgca aaaatgaaag cgctaacccg atttagtcga
     5221 caagttttta acagttcgtt attatatgaa tgtaagtaaa aatttcttag ctacaactta
     5281 catattataa atgcataaat taaacaaaaa ggggcgaaaa aagttgactc atttatcaga
     5341 tttagatatt gcgaatcaat caacactaca accaattaag gatattgctg catcagtagg
     5401 tatttcagag gatgcattag aaccttatgg tcattacaaa gctaaaatcg acattaataa
     5461 aattacgcca agagaaaaca aagggaaagt tgttttagta actgcgatga gcccaacacc
     5521 agctggtgaa ggtaaatcaa cggttacagt tggtttagct gatgcattcc atgagttaaa
     5581 taaaaacgtt atggttgcat taagagagcc tgctttagga ccaacatttg gtatcaaagg
     5641 tggtgcgact ggtggtggtt atgcgcaagt cttacctatg gaagatatca acttacattt
     5701 caacggagat ttccatgcga ttacaactgc aaataatgca ttgtctgcgt ttatagataa
     5761 tcatattcac caaggtaatg aattaggaat cgatcaaaga cgtattgaat ggaaacgtgt
     5821 attagatatg aatgatcgtg cacttagaca tgtaaacgtt gggttaggtg gacctacaaa
     5881 tggtgtacca cgtgaagatg gctttaatat tacagtagcg tctgaaatta tggcgatttt
     5941 atgtttaagt agaagtatta aagacttaaa agataaaatt agtcgtatta ctattggtta
     6001 cactagagat cgcaagccag ttacagttgc agatttaaaa gtgcaaggtg cacttgcaat
     6061 gattttaaaa gatgcaatta aaccaaactt agtacaatca attgaaggga cacctgcatt
     6121 agttcatggt ggaccatttg cgaatatcgc acacggttgt aactcaattt tagcaactga
     6181 aacagcacgt gatttagctg atatcgttgt aacggaagct ggatttggtt cagacttagg
     6241 cgctgaaaaa ttcatggaca ttaaagcgcg tgaagcagga tttgatctgg cagctgtcgt
     6301 tgttgttgcg acaattcgtg cgttaaaaat gcatggtggt gtagcgaaag ataatttaaa
     6361 agaagaaaat gtagaagcag taaaagcagg aattgttaat ttagagcgtc atgttaataa
     6421 tattaaaaaa ttcggtgtag aaccggttgt tgcaattaat gcatttatac atgataccga
     6481 tgcagaagta gaatatgtaa aatcttgggc taaagaaaat aacgtacgaa ttgccttaac
     6541 tgaagtttgg gaaaaaggtg gtaaaggtgg cgttgactta gcaaatgaag tattagaagt
     6601 cattgatcaa cctaattcat ttaaaccttt atatgaatta gaattaccat tagagcaaaa
     6661 gattgaaaag attgtgactg aaatctatgg cggttcaaaa gtaacgttta gcagtaaagc
     6721 gcaaaaacaa ttaaaacaat ttaaagaaaa tggttgggat aattacccag tatgtatggc
     6781 gaaaacacaa tattcattct cagatgatca aacgttgtta ggtgcaccat caggatttga
     6841 aattacaatt cgtgaattag aagcgaaaac aggtgcagga tttatcgtag cgttgacagg
     6901 tgcaatcatg actatgcctg gtttacctaa aaaaccagca gcattaaaca tggatgttac
     6961 tgatgatggt catgcaattg ggttattcta ataaatcatg tcaattgttt aataaagata
     7021 agtaaatagt ttaatagacc ggactgttgg agatgcatta tttcagcagt tcggtttttt
     7081 gctgtgctaa aaatagattc catttggcga atctaacgac aatgtttgaa ggtggttaat
     7141 taatgtatat gaagataaaa agtgggtttg aagaatagga aagcgatgca atgaatattc
     7201 atattaaaaa attttaataa aataggttgc aatatttaat tgggatgcgc tacaattaac
     7261 actaataatt gatattgata attattatca attaaatata atcttatagg agttgttaac
     7321 aacatgaaca aacatcaccc aaaattaagg tctttctatt ctattagaaa atcaactcta
     7381 ggcgttgcat cggtcattgt cagtacacta tttttaatta cttctcaaca tcaagcgcaa
     7441 gcagcagaaa atacaaatac ttcagataaa atctcggaaa atcaaaataa taatgcaact
     7501 acaactcagc aacctaagga tacaaatcaa acacaacctg ctacgcaacc agtaatcact
     7561 gcgaaaaact atcctgcagc ggatgaatca cttaaagatg caattaaaga tcctgcatta
     7621 gaaaataaag aacatgatat aggtccaaga gaacaagtca atttccagtt attagataaa
     7681 aacaatgaaa cgcagtacta tcactttttc agcatcaaag atccagcaga tgtgtattac
     7741 actaaaaaga aagcagaagt tgaattagac atcaatactg cttcaacatg gaaaaagttt
     7801 gaagtctatg aaaacaatca aaaattacca gtgagacttg tatcatatag tcctgtacca
     7861 gaagaccatg cctatattcg attcccagtt tcagatggca cacaagaatt gaaaattgtt
     7921 tcttcgactc aaattgatga tggagaagaa acaaattatg attatactaa attagtattt
     7981 gctaaaccta tttataacga tccttcactt gtgaaatcag atacaaatga tgcagtagta
     8041 acgaatgatc aatcaagttc agacgcaagt aatcaaacaa acacgaatac atctaatcaa
     8101 aatacatcaa cgaccaacaa tgctaataat caaccgcagg caacgacaaa tatgagtcaa
     8161 cctgcacaac caaaatcgtc agcgaatgca gatcaagcgt caagccaacc agctcatgaa
     8221 acaaattcta atggtaatac taacgataaa acgaatgagt caagtaatca gtcggatgtt
     8281 aatcaacagt atccaccagc agatgaatca ctacaagatg caattaaaaa cccggctatc
     8341 atcgataaag aacatacagc tgataattgg cgaccaattg attttcaaat gaaaaatgat
     8401 aaaggtgaaa gacagttcta tcattatgct agtactgttg aaccagcaac tgtcattttt
     8461 acaaaaacag gtccagtaat tgagttaggt ttaaagacag cttcaacatg gaagaaattt
     8521 gaagtttatg aaggtgacaa aaagttacca gtcgaattag tatcatatga ttctgataaa
     8581 gattatgcct atattcgttt cccagtatct aatggtacga gagacgttaa aattgtgtca
     8641 tctattgaat atggtgagaa catccatgaa gactatgatt atacgctaat ggcctttgca
     8701 cagcctatta ctaataaccc agacgactat gtggatgagg aaacatacaa tttacaaaaa
     8761 ttattagctc cgtatcacaa agctaaaacg ttagaaagac aagtttatga attagaaaaa
     8821 ttacaagaga aattgccaga aaaatataag gcggaatata aaaagaaatt agatcaaact
     8881 agagtagagt tagctgatca ggttaaatca gcagtgacgg aatttgaaaa tgttacacct
     8941 acaaatgatc aattaacaga tttacaagaa gcgcattttg ttgtttttga aagtgaagaa
     9001 aatagtgagt cagttatgga cggctttgtt gaacatccat tctatacagc aactttaaat
     9061 ggtcaaaaat atgtagtgat gaaaacaaag gatgacagtt actggaaaga tttaattgta
     9121 gaaggtaaac gtgtcactac tgtttctaaa gatcctaaaa ataattctag aacgttgatt
     9181 ttcccatata tacctgacaa agcagtttac aatgcgattg ttaaagtcgt tgtggcaaac
     9241 attggttatg aaggtcaata tcatgtcaga attataaatc aggatatcaa tacaaaagat
     9301 gatgatacat cacaaaataa cacgagtgaa ccgctaaatg tacaaacagg acaagaaggt
     9361 aaggttgctg atacagatgt agctgaaaat agcagcactg caacaaatcc taaagatgcg
     9421 tctgataaag cagatgtgat agaaccagag tctgacgtgg ttaaagatgc tgataataat
     9481 attgataaag atgtgcaaca tgatgttgat catttatccg atatgtcgga taataatcac
     9541 ttcgataagt atgatttaaa agaaatggat actcaaattg ccaaagatac tgatagaaat
     9601 gtggataata gcgttggtat gtcatcgaat gtcgatactg ataaagactc taataaaaat
     9661 aaagacaaag tcatacagct tgctcatatt gccgataaaa ataatcatac tggaaaagca
     9721 gcaaagcttg acgtagtgaa acaaaattat aataatacag acaaagttac tgacaaaaaa
     9781 acaactgaac atctgccgag tgatattcat aaaactgtag ataaaacagt gaaaacaaaa
     9841 gaaaaagccg gcacaccatc gaaagaaaac aaacttagtc aatctaaaat gctaccaaaa
     9901 actggagaaa caacttcaag ccaatcatgg tggggcttat atgcgttatt aggtatgtta
     9961 gctttattca ttcctaaatt cagaaaagaa tctaaataat taactaaata tagcatatgt
    10021 atggttaact ttgtaaacaa tgtgaaagca attaatttat aaactattga ttggtttaat
    10081 ggctttcctt tagagtaaat aaaaagaaca gcagtgagaa attttctaat tgaaaataat
    10141 cttactgctg tttttaatat ttggatgcat tgttgtggtt actttaaaaa gtgagcatca
    10201 attaacgctt ttttcgattt aacaaatgtg atttaatatc atattttaat gcgtcgttgt
    10261 attctttttc agtgatttga tcttcgatta acatacgctt taatacataa tgttgtcttt
    10321 gaatactata tttcaaatct ttatccgatt ttaacgttcc atctttttcg tagggtgtat
    10381 agccataagg gctttgcaac aaaccgataa ggtatgcaga ttgtgcaatt gataaatctt
    10441 ttggtggaat accaaacaaa ctatatgaag cggatgcaat tccggaaata ttagcgccat
    10501 tataatctct accgaaggga actatattta aatatgtata tataatttca tcttttgaga
    10561 gtaggtgttc taatctaatt gctaggcgaa gttcatttgc ttttctacta tatgtttttt
    10621 cgttggtaag aacttgattt ttaacaagtt gttgtgtaat tgtgctacca cctgaacttt
    10681 gatcagtatt aaaaatatct tgtatcattg ctcttaaaat cgcctttggt aagatgccat
    10741 catgtttata aaataaagtg tcttcagatg atgttaatgc tttaatgaca tttggacttg
    10801 atgttttagg gcctataatg agtgagtttt gagaatggtt atactcatat aataaatttt
    10861 tgttattatg atctaataat tcatcgccag gtatttgtcg aacttttttt attaaagcat
    10921 catctgataa tgagtcggac gttttagtta aatgatgaaa atataaagac atcgcaatca
    10981 cagcgatagc aacgatggca atgaaaatca taaatataaa aacaatgaca tgtttgatag
    11041 ctttatatat cttttcaaaa tgatatatac gatgattcaa ttgatgattg ttgtcttgat
    11101 tcgtcatctt taacctccta aattacgtat agtataccat atttaagcaa tttgactttt
    11161 attttaacgt taattataat tagggaaaat gcataaagaa taggataaaa ggacaagtaa
    11221 tattatttga aatcatcaga gagctagtgg tcgctgtgaa ctagtgtttt taataatatg
    11281 aatacacctt tataactaac atgtcctacg tttattaatg aacgtatcat taatagagtg
    11341 aacattatct tatgattatg tttaagtttg gtggtaacgt gcaatcgcgc ccttacatat
    11401 ttgttgtaag ggtttttttt acgttcaaaa aactttaaag tattttaaca agtattcaat
    11461 aaataattat aaataggagg aaataataat gacgaatgta ttaattgaag atttaaaatg
    11521 gagaggtctt atttatcaac aaactgatga acaaggtatt gaagatttat taaataaaga
    11581 acaagtgacg ttatactgcg gtgccgatcc aacggcagat agtttacata ttggtcactt
    11641 actaccattc ttaacattaa gacgttttca agaacatgga catcgtccta tcgttttaat
    11701 tggcggtggt acaggtatga ttggtgatcc atcaggtaaa tcagaagaac gtgtgctaca
    11761 aacagaagaa caagtagata aaaatatcga aggtattagt aagcaaatgc acaatatttt
    11821 tgaatttgga acagaccatg gtgcagtgct tgttaataat agagactggt taggacaaat
    11881 ctcattaatt agctttttac gtgactatgg taaacacgtc ggcgttaatt acatgttagg
    11941 taaagattca atccaaagtc gtttagaaca tggtatttca tatacagaat tcacatacac
    12001 gattttacaa gctattgatt tcggtcattt gaatagagaa ttgaattgta agattcaagt
    12061 aggtggatca gatcaatggg gtaatatcac aagtggtatt gaattaatgc gtcgtatgta
    12121 tggtcaaaca gacgcatacg gtttaactat tccgcttgta actaaatcag atggtaagaa
    12181 atttggtaag tctgagtcag gtgctgtttg gttagatgct gaaaaaacaa gtccttatga
    12241 attttatcaa ttctggatta atcaatcaga cgaagatgta attaaattct taaaatactt
    12301 tactttctta ggaaaagaag aaattgatcg cttagaacaa tctaaaaatg aagcaccgca
    12361 tttacgtgaa gctcaaaaaa cattagctga agaagtaact aaatttattc atggtgaaga
    12421 tgcattaaat gatgcaatcc gtatttcaca agcattattt agtggtgatt taaaatcatt
    12481 atcagcgaaa gaattaaaag atggatttaa agatgtgcct caagtgacat tatcaaatga
    12541 cacaacaaat atcgttgaag tccttattga aacaggcatt tctccttcta aacgacaagc
    12601 acgtgaagat gttaacaatg gtgcgattta tattaatggt gagagacaac aagatgttaa
    12661 ttatgcttta gcaccagaag ataaaattga tggcgaattt acgattattc gtcgcggtaa
    12721 gaaaaaatac ttcatggtta actatcaata aatataattg catagctaaa taaattagag
    12781 cctactcata ttcattccta agaatgtaat gagtaggctc ttaatgtact tttctgtctg
    12841 taaattatct aaagaaatct ctatcgcctt ggccaggtga ttgacgttct gatcggcttt
    12901 gacgttttgg ttgttctttt tgttgtttta atttcacttt aacttctttt gttttaccat
    12961 cacggataac ggtaacagtg actgattcac caggtttttt attttcatat aaatagcttc
    13021 ttaaatcaac atcatcttta attttcttgc catcaatttc tgtaataata tcaccttttt
    13081 taagatcaat atcactatca gctttggcga cataaatacc gtcttctcta tcagtatgaa
    13141 gttgctcgcg ctcttcttca ggaatatctt tcaaattaat taaaccaata ccaatcgaag
    13201 ggcggtcaat tttaccatgt tttacaagtt gttcaattgt tactttaact tcattacttg
    13261 gaatagcaaa cccgatacct tcaacttgtg tcgcagcaat tttcattgag ttaataccaa
    13321 ctaaattacc attaatatct actaatgcgc cacctgagtt acctgggtta atagcagcat
    13381 ctgtttgaag aacgctaact tttgtattgc caccagttgt ctcagcgtca atcgtacgtt
    13441 cgcttgctga aatgatacca gatgttacag agttagcaaa ttgtaatcct aatgggttac
    13501 ccattgcgaa tacgctatcg ccagtttgta cttttgaaga gttggcaaat tgaatcgctt
    13561 taataccttt tgtattttca attttaagta cagcaatatc agttactgca tctttaccaa
    13621 ctaatttcgc tttaacttgt tttttattat gtaattggac tctaatttca tttgcgccat
    13681 caataacatg attgtttgta acgatataag ctgaattgtt gtttatttga tagataacac
    13741 ctgaacctac tccagcttca gatggtttag atgatttgcc ttttaataag tcgtctacac
    13801 ttgatgcttt ttgcatgtta ataactccaa caattgtagg ggagacagat tttatcattt
    13861 catgaacggt accgaatttc ttgctttgac cgtctaattg attgccacct ttattatttg
    13921 ttgtctgaac agttgaacca tctttattta aaattgtact atttaatact ttgcctatac
    13981 caagtactag aagtgcacca ataattccag caatcaatgc aacgatgact gttttaaacc
    14041 atggaaattt aggtcttctg tatctaggtg tttggctatg gtttgttgta gaatgatctg
    14101 tatgattaaa atctgacata cttaacctcc attatatgat ttatatatgc tttaattatg
    14161 tctttttttt ataattattg ctagtaatac actatacaac tttagatgta ttttcagact
    14221 atcttgttct agtaaaaata tagtgataaa atctagttgt tataaacaaa taagttaagt
    14281 aggtggcaca atgtattcag tgattagtaa gattttgaat ttcattttag ttaaaatgtc
    14341 aaaatcatta tacgtaatcg gtaaagataa tattcctaag gatagtaaat atgtcgtaac
    14401 ttgtacgcat gaaagttata acgaagttat tatgctaggt atggcattat atcctaatca
    14461 aattcattat atggctaaaa aagaattatt caaaaataaa tggattggta aatttttaac
    14521 gtctttaaat gcatttcctg tagatcgtga aaatccagga cctagtactt taaaacgccc
    14581 aatcaatttg ttgaaagata ataagacagt tggtatcttc ccaacaggtt ctcgcacatc
    14641 gcaagaaggt gcaccattga aacgtggtgc ttcaacaatt gcaatgttaa gtaagtcacc
    14701 aatattacct gttgcttatg ttggtcctac taagattcat ggtcttttaa caggacaagc
    14761 atacataaat attggtaaac caatcgatat taacgattta cctaaagatt taaaacgaaa
    14821 cgagcgtatc gattacatta caaaagagat tgaaacacga acagcaaagt tacaacaaga
    14881 attgcatgaa attgtaaaaa gtttataaaa taacttgagg tagaaatata gctggttagt
    14941 atgcctaggt gactataacg gagtatcgaa gtagttattc tagttacatt agtaatcttt
    15001 atatttctac cttattttaa gaaggatgac attcaatgta taaatttttc caaaatttag
    15061 ggcgttcgtt aatgttacca gttgccattt taccggctgc agcaattatt gccggaattg
    15121 gtaatacgtt aaatgcactt catgcgacgc cgaaaatcgc aatgttcttt actacggtag
    15181 gtactacgat tttagagcaa ttaggtattt tatttgcaat tggtgtagct ataggaatgg
    15241 caaagaaaaa tgatggtgca gtcgcgcttg cagctacgtt aggatatttt ttagttacag
    15301 ttgtattatc accaatgaaa ttagcgcctt tattaggaat gaaagcttcg gaaataaatt
    15361 cagcttttga aaagatgaat aatggcaatg tctttgttgg tattgtaata ggtttaatag
    15421 cagcctatgc atacaataag tttagtgaaa cagaattacc attagcatta tcatttttta
    15481 gtggtaaacg tctagttcca attatgactg cattttactg tacattttta gttgtcatat
    15541 tgttattctt atggccacta ctttattcat ggattgtaaa atttggtgaa tctatagtcg
    15601 gtttaggatc gtttggagct ttcatatatg gtgttgccaa tagattatta attcctacag
    15661 gattacatca tgcactgaat agtgtgttct ggtttgatac gattggtatt aatgatattg
    15721 gtaaattcca aagtggcaaa gatgctataa aaggtattac aggacgctat caagcgggat
    15781 tcttcccaat catgatgttc ggtattccag cggctgcgtt agcaatatat catacagcta
    15841 aaacgactca gaaaaaacaa gtttacggtt ggttcctagc gagttcagtt gcagcatttt
    15901 tcgttggtgt tacagagccg attgagtttg catttatgtt tgttgcgcca atcttatatg
    15961 ttgttcatgc gttattaaca ggattatctt tatttatcgc agctacattc cattggacag
    16021 ctggattttc atttagtgca ggtttgatag attatgtgtt atcactaatt aatccagtat
    16081 caaatcatcc attgatgttg cttgttcaag gtgttgtatt tttcatctta tactatgtga
    16141 ttttccgtgt agtaatccaa gtatttaact tgaatacgat tggtagaggt gaaaatgaat
    16201 tagttgatcc aacagttgta aaagataata ttgctcctgg tgaaaatgat attaaacaaa
    16261 gtaaatatca tcaacatgct atacaaatat tagaaggttt aggcggtcaa gagaatattg
    16321 ttaatttaac caattgtgca acaaggttgc gtctagagtt aaaagacaca tctattattg
    16381 atcaacagaa gattaaaaac gccggtgcag ttggtgttac tgttaatggc aaacattcaa
    16441 ctcaagttat tgtcggtaca cacgtacagc aggttgctga tgaaattgaa aaacatttat
    16501 aaaatttaaa cagctaaagc gcaaatctaa cttcaatagg gtttgcgctt tttttagtca
    16561 aatagtagaa tgatttatat ctgaattttc agaaatggag tggggattgt gaagaaaatt
    16621 ttatttgatg ttgatggcat ttttttaagt gaagaacgtt gtttcgatgt ctctgcatta
    16681 acggtatatg aattattaat ggataagtgt tatttaggtc ttcattcaca tatagattgg
    16741 gaaactttga ctgataacga tatacaagac attaggaatc gaatttttca aaaagataag
    16801 attttaaaca aattaaaatc gctagggctg aattcaaatt gggatatgtt atttatcgtt
    16861 tttagtattc atttaattga tattttgaaa aagttatcac atgatgaaat tgaggcattc
    16921 atgtatcaag atgagcctgt cgaattgaag ttgcaaaata taagcacaaa cttagcagac
    16981 tgttttaatt taaatgaaca actaccttta caatttttgg ataatgtaaa agttggtaaa
    17041 aataatattt atgctgcttt ggaagagttt gcaacaacag agttacatgt ttcggatgct
    17101 actttattta gtttgaaggg tgcattatgg acgttagcgc aagaagttta tcaagaatgg
    17161 tatttaggat cgaagttgta tgaagatgtt gaaaagaaaa tagcacgaac tacttttaag
    17221 acaggttata tttatcaaga aattattttg agaccagtag atgaagttaa ggtacttctg
    17281 aatgatttaa aaggtgctgg tttcgaatta ggtattgcaa caggtcgtcc ttatactgag
    17341 actgttgtgc catttgaaaa tttaggattg ttaccatatt ttgaagctga ttttattgca
    17401 acagcaagtg atgttttaga agcagagaat atgtatccgc aagcacgacc attaggaaag
    17461 ccgaatcctt ttagttatat cgcagcttta tatggtaata atcgcgataa atatgaatct
    17521 tatatcaata agcaagataa cattgtaaat aaagatgacg tatttatagt aggcgattcg
    17581 ttagctgact tattaagtgc tcaaaaaata ggtgcaacgt ttattggaac attaacaggt
    17641 ttaaaaggta aggatgctgc aggtgagtta gaagcgcatc atgccgacta tgttattaat
    17701 catttaggtg aacttagagg tgtactagat aatttgtaat ttgattgttg tttgacagca
    17761 taacttgaag tgaatgattg aaccaaaggt ttcatattga gttacaatga aattaataat
    17821 gaaaaaatgc caagaagcaa tggaagtaat ccaatgtctt cttggcattt tgaatttaca
    17881 taaattgttt atgactgtac cgtcaattca gttgtgaaaa tttgattgta ttcaccaact
    17941 tgtttaagtt catcaattat attgtttgaa acaggttgat caacggataa aatcattagc
    18001 gcatctccgc ccgcttcagt tctacctaaa gtcatagatg caatgttgat attgtattta
    18061 cctaacaatg cgccagtttt tcctaccata cctggagtat cattatgata tgacacaatt
    18121 tgatattgat ttggctttaa gtctacagaa aaattattaa ttctaacaat tcttggaccg
    18181 aaacctgtaa agacagaagc gccaacttta acggaatcgc tatcgtttga tagttctacc
    18241 tctaagtagt tactaaaacc tgtctctgct ttattatttt caatatttaa tgtcacttgt
    18301 tgttgattta acaacattag agcattgata atattgactt caggaccaag atcatctttt
    18361 aaaatatgag taataattgt acgtgttaat aaactactat caatagaggc taagtcgcca
    18421 ccgtacgtaa ttttaataga gcttggtgca ttgtacatta attgaatagc taattcacca
    18481 actgtttggc ttaaattgat gaatgatttt acagtatcat ctatattgct taagtccatt
    18541 ttaggtgcat tcactgcatg cgttacagta ccatcaatta aaatttcgat gatttcattt
    18601 gaaacagaaa ttgccacttt ttcttgagct tcgactgttg atgcacccaa atgaggtgta
    18661 acaataattt tatcatgtgc aacaagaggc gaatcagttg caggttcatg ttcaaacaca
    18721 tcgatagctg cccgactaat ttgtccttcg tctaatgctt ttattagcgc cttttcatca
    18781 ataataccac cacgtgccac attgattatt tgcaaactag gttttgcttt ggcaaaaaag
    18841 tcagcattaa ttaagccttt tgttttaggt gttagtggtg tatgtaatgt aacgaaatca
    18901 gaatgttggg caatctcatc aactgttgcc ttcgtaatac ttaaagattt tgctttttca
    18961 tccgttaagt aagggtcaaa agctagtatt ttcattccga aactttgcgc acgtttagca
    19021 acacctaaac caattctacc agcaccaatg acacctaatg ttttatgata aagctcagta
    19081 cctttaaatg catttcgatt ccattcttta tttgtaagtg attggtgtgc ttgcggaata
    19141 tttcgagcca ttgataataa cattgccagt gaatgttcag tagctgaaat cgtattacca
    19201 tctggggcat taataactaa aatacctttt aatgttgcag cattaatatt aatattatct
    19261 acaccaacac cggcgcgtgc gattactttt aaagaatcag cagcatttat gatattttca
    19321 gtaaccgtag tttgactacg aacgattaaa gcatggtatg aaggtataat tttgattaat
    19381 gcttcttcgg acaagccagt ttgaatatct acattgaatt gttcgtgatc taataatgct
    19441 ttgataccat cttttgatat gggatctgca acgagtacat taaattgctt catgaataac
    19501 ctccatatat tttgatatac ctttaccgat atagttaact ttacggtgtt cagttaaaat
    19561 aatttctaaa gcagatacta ccgataaaat atcgaaagga ctaattttcc ccatatgacc
    19621 aattcttaaa atttgacctt taagatggcc ttgaccacca gcaattgtta ttttaaagcg
    19681 atttttaagt tcatctttga ttattttcac ttcatttgta ttaggtttga atgctgtaac
    19741 cgttggagat gcatctttat cattgactaa taaagttaaa tctaatgctt ttaaggcgct
    19801 tcttaatgca tttctaattg catagtgtcg tgctattacg tgattgaaac cttctgcttt
    19861 tacggtttca acgtatgcat ttacacctct aaataaagac acatttggtg tgaacggtgt
    19921 agaattgtca gcttgtgacg aaatgtattt atttaagtct agataaaatt ttggcgtagt
    19981 tacttctttg aaacgttctt ttgcacggtg gctataagct acaaaagcta atcctggagg
    20041 taacataatt gctttttgac taccagaaac aagtacatca attttatctt tgttaatgtc
    20101 aacatcaaca gcaccaatgc aacttacgcc gtcaactaca aaataaatat tactattaaa
    20161 ttgattgatg gcatttccta actcgtgaat agggtgtagc actgtcgtag atgtttcgca
    20221 atattgacta aatactgctt taacttcaac atctaaagtt gaaaggtaat tgatgaaatc
    20281 tttaacatct acagcttctc cccatgttac gtcataaatg tgcacatttt tgtaataagt
    20341 ttgtgcaatt tgtttaaatc ggttaccaaa ggcacctgaa acaatgacaa cgaagtgatc
    20401 ttcagggttt acaatgttca acatactagc ctccaagacg cttgtaccgc tagatgttaa
    20461 aataagtaca tcattttgac tcccaaatat tggctttaga ccttgaaatg cttgttgtgc
    20521 gatgtcttca aaatctttag aacgatgacc aaccataggt gcttgaattt ctctcataat
    20581 ggcatcaggt aaaggggttg ggccaggtgt taataacaac ggttgatgat aatacattta
    20641 tatgcctcct ataaataaga tttcactatt ttagcaaatt ttctgaaaat ttaaaagcct
    20701 aaaaaagtga taacgctata ttgtcgaata aacgttatca cgtaacgatt ttatttggcc
    20761 tgaatgatgg gatagatttt tatgtctaca ttatttctta cagcatttga aatcatgcaa
    20821 ttattatctg caattgttat caattttggt aatcgctttt ctaattgtgc tatttgatca
    20881 cttggaattt caatgtgagg atggtgcaca atttttgaca tactgaattt tccattattt
    20941 aaacaagctg ttccaatcga ttgttgttca attgaaatat ctgtgaactt tgcacgttca
    21001 agagtagctg ctaatgagat gatataacat gatgaagcgg ctgatactaa catttcatcg
    21061 ggatttgttc ctataccaac accacctaaa gaagcaggta ttgaaatatt ctctgaaagt
    21121 atgtcgcctt gaacgtttcc gacattgtta cgaccacctt gccaagaagt ttggacttta
    21181 aagtcatgtt gatgcaattg ctttaacctc caatataatt gtgatagttt aattttagaa
    21241 tagtttatca aagtttaaca gaaaggtgac ttatcaatga ctctgaataa actgaaagat
    21301 gaattacaaa ttgtttcgca ccgtggatta ccgagtgatt ttcctgaaaa tacaatggtc
    21361 ggttatcgag aggtaatggg gctcaatgtt gctatgttag aaatagatgt tcatttgacc
    21421 aaagaccaac attttgttgt gatacatgat gaaacaattg atagaacatc ggatggtaag
    21481 gggcgtattg ctgattacac attatcgcaa ttaaaatcat ttgattttgg tagttataaa
    21541 gatgttgctt ttaagggaga acgaataccc acgttagatg aagtactttc attatgctta
    21601 aagtatgata aaaagctatt gattgaattg aaaagtccga atttatatcc agaaatagaa
    21661 tgtaaattgc tggcattttt ggaagaaaag aaagtcgatg cgacacaagt agttatacaa
    21721 tcttttgata tcgaatgcat cgaaaagtta aacacattgg gcagtatata tgaattaggt
    21781 gtgctttgta gtaaacgtaa ttattggtat aaaaagccaa acttttcaag aattgctcaa
    21841 attgctagtt atgttaatcc taattatgca ttagtggcca gaaaatttgt tgataaagca
    21901 catcatcatc aattacaggt tatgccatac acagttaata aattgaaaac tggagaaaaa
    21961 ttaagacaaa tgggtgtaga tggattaatt acagataatc cgaaattatt tattaagtga
    22021 gacgatgaat ggtaatttga tagagggatg aggattcgta tatcgcaatt gataatttta
    22081 aaaactaatg atgaaatact aaagataatt aaacaaataa gacttgtaaa ttctaagttg
    22141 attgtaaaat agggtagctc catataattt tgaatctatc atttttaaat taataaaaaa
    22201 aacacccaca attgtgggtg tttgtattga acgtattatc ttgagtagta ctcaacgatt
    22261 aattgttcgt taatttcagc aggtaattcg ctacgttctg gtaaacgtac gaaagtacca
    22321 gttaagctgt cagcatcaaa gtttaagtac tcaggtacga aattgttgat ttcaactgat
    22381 tcaacgatga tgtttaattt ttgagatttt tcacgaactg aaattgtttg accaggttta
    22441 acagaataag atggaatatc aacacgttta ccatctacta agatatgacc gtggttaact
    22501 aattgacgtg cttgacgacg agtacgagct aaacctaatg aataaacaac agcgtctaaa
    22561 cgacttgcta ataaaatcat gaagttttca ccgtgtacac cgaatttttt accagcgatg
    22621 tcaaatgtgt tacggaattg tctttcagtc attccatata agtaacgtaa tttttgtttt
    22681 tcacgtaatt gtaaaccata ttctgataat tttttacgtt ggtttggacc atgttgtcct
    22741 ggtgcgtaag gacgtttttc taattcttta ccagtaccgc ttaaagagat acctaaacga
    22801 cgagattttt tccagtttga acctctgaat cgagccataa taagactcct cctttaattc
    22861 ttttttgttg ttatgaataa acaaaaaaga gtgttgcatg cttactaaga tatattgttt
    22921 tatgtgtctt caccttatag cgacagttac gtgacacgtc cgcattggga acaacatagc
    22981 gctcaataat atacaactgc tactttcgtt aattcacaca aagattattg taacattttc
    23041 ctgctaactg tcaacacttt attgaaaatg ctgatgtcct tttatgcgag ttgcttttca
    23101 ataattttaa caattgcttc aagatgttct ttgtcattgt catcaaatcg atccgttata
    23161 ggggcatcga tatctaagac gccgataatt ttatcatctt tgaaaattgg aacgacaatc
    23221 tcagacttac tattagcatc acaagcgata tgtccttcga attgatggac atcagctaca
    23281 acttgtgtac gacgttctga aacggctgta ccgcatacac cttttccaat tggaatgtgg
    23341 acacaagcgg ggtgtccttg gaaaggtcca agtataagtt cattttgttc caataagtaa
    23401 aagccaaccc aattaatttg atttagatta tcatttaata aggcagacat gttacttaaa
    23461 atcgcaatca tatgatgttc atcttcaata aggcttgctg cttgtttctt taataatgtg
    23521 tagtttgttg ggttaattgt tgtcatattt aaaacctctt attttcatag attttataat
    23581 agtatagaat ttcgaaaata ttatggcaat aaattcattt tataatttat ccttaagcaa
    23641 aattacgtta taatagtaat gataaattag gaggagaagc atatggtgtt atatatcatt
    23701 ttggcaataa ttgtgattat attgattgct gtaggtgtat tattctattt acgttcaaat
    23761 aaacgacaaa ttattgaaaa agcaatcgaa cgtaaaaatg aaattgagac gttacctttt
    23821 gatcaaaacc ttgcacaatt atctaagttg aatttaaaag gtgaaacaaa aacgaaatac
    23881 gatgcaatga aaaaggacaa cgtagaaagt acaaataagt atctagctcc tgtggaagaa
    23941 aaaatccata atgctgaggc tttattagat aaatttagtt tcaacgcatc tcaaagtgaa
    24001 attgatgatg caaatgagtt gatggatagt tacgaacaaa gctatcagca acaattagaa
    24061 gatgtaaatg aaattattgc gttatacaaa gataatgatg aattatatga caaatgtaag
    24121 gttgattatc gtgaaatgaa acgtgatgtt ttagcaaatc gtcatcaatt tggtgaggca
    24181 gcaagtctac ttgaaactga aattgaaaag tttgagccaa ggttagagca atatgaagta
    24241 ctaaaagctg atggtaatta tgtacaagcg cacaaccata tagctgcctt gaatgaacaa
    24301 atgaaacagc taagatctta tatggaagaa ataccagaat taattagaga aactcaaaaa
    24361 gaattacctg gtcaattcca agatttaaaa tatggttgcc gtgatcttaa agttgaaggg
    24421 tatgatctgg atcacgtaaa agtagacagt acattacaaa gcttaaaaac agagcttagt
    24481 ttcgttgaac cattaattag ccgcttagaa ttagaagaag ctaatgataa actagctaat
    24541 atcaatgata agttagatga catgtatgat ttaattgaac atgaagttaa agctaaaaat
    24601 gatgtcgaag aaacaaaaga tatcattacg gataacttat tcaaagctaa agacatgaat
    24661 tatacattgc aaacagaaat tgaatatgta cgtgaaaact actatataaa tgaatctgat
    24721 gctcaaagtg ttcgtcaatt tgaaaatgaa attcaaagtt taatttctgt atatgatgat
    24781 attttaaaag aaatgtctaa atctgctgta cgatatagcg aggttcagga taatttacaa
    24841 tatttagaag atcatgtcac agttattaat gacaaacaag aaaagctaca aaatcatctg
    24901 attcaattgc gtgaagatga agcagaagca gaagacaatc tgctacgagt acaatcgaag
    24961 aaagaagaag tgtatcgtcg attacttgct tctaacttaa caagcgttcc tgaaaggttt
    25021 atcatcatga aaaatgaaat tgatcatgaa gttcgtgatg ttaacgaaca atttagtgaa
    25081 cgtccaatac acgttaaaca gttaaaagat aaagtgtcta aaattgtgat tcaaatgaat
    25141 acatttgaag atgaagcaaa tgatgttctt gttaatgctg tttatgcaga gaaattaatt
    25201 caatatggaa atagatatcg taaggactat agcaatgttg ataagagctt aaatgaagct
    25261 gaacgattat ttaaaaataa tcgctataag cgtgcgattg aaattgcaga gcaagctctt
    25321 gaaagtgttg agccaggtgt cactaaacat attgaagaag aagttattaa gcaatagaaa
    25381 ctagtatgta gttatactta aataatatga gcactctgtc aaattggact gatgagttta
    25441 ataattgaag ttagccaacg atacgttgtc tagcttcttt ttttatatgg ataaatgaaa
    25501 gggacaataa atataaatag caattgttta aagataaacg taatcaaatg tgttgtttta
    25561 attaatataa gtagtgaaaa aagcataatc acacagctgt ttaaatagag tgaaatagtc
    25621 taattcttat ttaataagta gaaataagat tattttgcgc tataaccttt attttttgtc
    25681 ggataatagt ataatgcttg agtaaaacaa ttttaacgat tgatgattaa tgaaagagaa
    25741 gtgtttattt tgatatatct agataatgcg gcaacgacga aagcatttga agaagtgtta
    25801 gatacttatt taaaagtaaa tcaatcaatg tattataatc cgaatagtcc gcataaagct
    25861 ggtttgcagg caaatcaatt actacaacaa gcaaaagccc aaattaatgc aatgattaat
    25921 tcaaaaacaa attatgatgt tgtattcact agtggtgcaa ctgaatccaa taatcttgct
    25981 ttaaaaggta ttgcctatcg taaatttgat acagcgaagg aaataattac atccgtgtta
    26041 gagcatccgt ccgtattaga ggttgtaaga tatttggaag cacacgaagg atttaaagtt
    26101 aaatatgttg atgtaaagaa agatggcagt attaacttag aacacttcaa agaattagtg
    26161 tcagacaaag tcggtttagt aacatgtatg tatgtaaata atgtaactgg acaaatacag
    26221 cctattccac aaatggctaa agttataaaa aattatccta aggcacattt tcatgtagat
    26281 gcggttcaag cattcggcaa aatttcaatg gatctcaata acatagatag tattagttta
    26341 agtggacaca agtttaatgg tttaaaagga caaggcgtct tacttgtaaa tcacattcaa
    26401 aatgttgaac caactgtcca tggtggtggt caagaatatg gcgttagaag tggaacagtt
    26461 aatttgccaa atgatattgc aatggttaaa gcgatgaaga tagctaatga aaactttgaa
    26521 gcattgaatg catttgttac tgagttaaat aatgacgtcc gtcaattttt aaataaatat
    26581 catggagttt atattaattc ttcaacttca ggttcaccat tcgttttaaa tattagtttt
    26641 cctggcgtaa aaggtgaagt attagttaat gctttttcaa aatatgacat tatgatatct
    26701 acgacgagtg cttgttcatc taaacgtaat aaattaaatg aagtattggc tgcaatggga
    26761 ttatcagaca aatctattga aggtagtata agattatcat ttggggctac tacaactaaa
    26821 gaagatatag caaggtttaa agaaatattt atcatcattt atgaggaaat taaggagttg
    26881 ctaaaataat gaagtatgat cacttgcttg ttagatacgg ggagttaaca ttaaagggtt
    26941 caaatagaaa aaaatttgta aatcaattaa gaaataatgt aaataagtca ttaaaaggac
    27001 ttgatgggtt tgtcgttaaa ggcaaacgag atcgtatgta tattgaactt gaagaccatg
    27061 cagatataaa tgaaataaca tatcgattat caaaaatttt cggtattaaa tctattagtc
    27121 cagtattaaa agtagaaaaa acaatagagg caatgagtgc agcggcaatt aaatttgcgc
    27181 agcaatttga agaaaacagc acatttaaaa ttgatgtgaa gcgtgccgat aaaaatttcc
    27241 caatggatac gtatgaatta caacgtgaat tgggtggtac cgtattgaaa caaattgaaa
    27301 atgtttctgt taatgtcaaa cgtccagatc atgaaattcg agtggaagtt agattagatg
    27361 caatttatat gtatgaagaa gttgttccgg gttcaggcgg attaccagtt ggtactggtg
    27421 gtaagacgtt actaatgctt tcaggcggta tagactcacc agttgctgga atggaagtga
    27481 tgagacgtgg cgtaacaatt gaagcgattc atttccatag tccaccattt acaagtgatc
    27541 aagcaaaaga aaaagttatt gaattgacac gtattttagc tgaacgtgtt ggaccaatta
    27601 aattgcatat tgtaccattt acagaattgc aaaaacaggt aaataaagtt gtacatccaa
    27661 gatatacaat gacttcaacg agacgtatga tgatgcgtgt tgctgataaa ttagtacatc
    27721 aaataggggc tttagctatt gtaaatggtg aaaacctagg gcaggtagcc agtcaaacac
    27781 ttcatagcat gtatgcaatt aataatgtaa cttctactcc tgtattacgt cctttattaa
    27841 cttacgataa agaagaaatt attattaaat cgaaagaaat tggtacattt gaaacatcta
    27901 ttcaaccatt tgaagattgt tgtacaattt tcacccctaa aaatccagta accgaaccaa
    27961 actttgaaaa ggtagtccaa tatgaaagtg tctttgattt tgaagagatg attaatcgtg
    28021 ctgttgaaaa tattgaaaca cttgaaataa ctagtgatta taaaact
//
LOCUS       DAOBBH010000016        26070 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.016, whole genome shotgun sequence.
ACCESSION   DAOBBH010000016 DAOBBH010000000
VERSION     DAOBBH010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 26070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26070
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.016"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..190)
                     /locus_tag="P6L01_000467"
     CDS             complement(<1..190)
                     /locus_tag="P6L01_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDP3029561.1"
                     /translation="MNNYKIGQHIKVRVTGIQPYGAFVETPNHTEGLIHISEIMDDYV
                     HNLKKFLSEGQIVKAKILS"
     gene            complement(605..1198)
                     /locus_tag="P6L01_000468"
     CDS             complement(605..1198)
                     /locus_tag="P6L01_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDP3029562.1"
                     /translation="MANYPQLNKEVQQGEIKVVMHTNKGDMTFKLFPNIAPKTVENFV
                     THAKNGYYDGITFHRVINDFMIQGGDPTATGMGGESIYGGAFEDEFSLNAFNLYGALS
                     MANSGPNTNGSQFFIVQMKEVPQNMLSQLADGGWPQPIVDAYGEKGGTPWLDQKHTVF
                     GQIIDGETTLEDIANTKVGPQDKPLHDVVIESIDVEE"
     gene            1262..1645
                     /locus_tag="P6L01_000469"
     CDS             1262..1645
                     /locus_tag="P6L01_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase-associated lipoprotein B"
                     /protein_id="HDP3029563.1"
                     /translation="MKLYRFSHKTGSYGVTIVEENGDDVLVKVEQVIKHPKQGDLHNP
                     TETEGVFFHERKALSHFEKRYTKRSQLRDFNVDVLRYEDSLQQAITDLETKLKAQQTK
                     HAEMSLASLARLKEDYSLQYKENFK"
     gene            1776..4181
                     /gene="mnhA1"
                     /locus_tag="P6L01_000470"
     CDS             1776..4181
                     /gene="mnhA1"
                     /locus_tag="P6L01_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit A"
                     /protein_id="HDP3029564.1"
                     /translation="MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIY
                     MLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYL
                     SKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIY
                     GAQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIG
                     AFTKSAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTV
                     TLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYA
                     AAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITAL
                     SMAGVPPFNGFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFI
                     MHIFFGQYKPEQLPKKAHEVSILMLLSPAILATLVIVLGLFPGILTNSIIEPATSSIN
                     HTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFNYWYNR
                     SANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTIFSVPFNINFKDVSPIRIF
                     EVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESIS
                     TALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSVIIIGLIAYGNRHFESISKFY
                     QEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVK
                     NHE"
     gene            4174..4602
                     /gene="mnhB1"
                     /locus_tag="P6L01_000471"
     CDS             4174..4602
                     /gene="mnhB1"
                     /locus_tag="P6L01_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit B"
                     /protein_id="HDP3029565.1"
                     /translation="MNRQQNDLILQFAAVIIFFMVMVFGFSLFLAGHYTPGGGFVGGL
                     LFASSLVIITIAFDIETMRKIFPLDFKILIGIGLVFCIATPIASWFLGKNFFTHVTFD
                     IPLFILEPVHMTTAVFFDFGVLCAVVGTVMTIIISIGENE"
     gene            4602..4943
                     /gene="mnhC1"
                     /locus_tag="P6L01_000472"
     CDS             4602..4943
                     /gene="mnhC1"
                     /locus_tag="P6L01_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit C"
                     /protein_id="HDP3029566.1"
                     /translation="MEIIMIFVSGILTAISVYLVLSKSLIRIVMGTTLLTHAANLFLI
                     TMGGLKHGTVPIYEANVKSYVDPIPQALILTAIVIAFATTAFFLVLAFRTYKELGTDN
                     VESMKGVPEDD"
     gene            4936..6432
                     /gene="mnhD1"
                     /locus_tag="P6L01_000473"
     CDS             4936..6432
                     /gene="mnhD1"
                     /locus_tag="P6L01_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit D"
                     /protein_id="HDP3029567.1"
                     /translation="MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLV
                     AAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFSSLLIITSIIITAIVILYSYQTI
                     GIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVFFEVFLMSSYFLLVIGSTKIQLQE
                     TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIF
                     VFATKAGVFPMFVWLPSAYYAPPIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSH
                     YVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVAMMTESGMIGAIYYT
                     LHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSG
                     FYGKFFIVQSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVK
                     YLTTIAIVAVVITVLYGLSADYLYPMVKAGAETFYNPSTYVKAVLGGK"
     gene            6434..6913
                     /gene="mnhE1"
                     /locus_tag="P6L01_000474"
     CDS             6434..6913
                     /gene="mnhE1"
                     /locus_tag="P6L01_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit E"
                     /protein_id="HDP3029568.1"
                     /translation="MAVQLVLNFIIAVFWLFVTNSYTTNNFVLGFIFGLVLVYLLHRV
                     LPGRFYVITLYRIIKLVIIFLIELIKANFDVLKIIIKPSIKNEPGFFVYHTDLKKDWQ
                     IVLLSNLITLTPGTVVLGVSDDRTKIYIHAIDFSTKEQEVESIKTSLEKIVREVGEI"
     gene            6913..7206
                     /gene="mnhF1"
                     /locus_tag="P6L01_000475"
     CDS             6913..7206
                     /gene="mnhF1"
                     /locus_tag="P6L01_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit F"
                     /protein_id="HDP3029569.1"
                     /translation="MNHNVIIVIALIIVVISMLAMLIRVVLGPSLADRVVALDAIGLQ
                     LMAVIALFSILLNIKYMIVVIMMIGILAFLGTAVFSKFMDKGKVIEHDQNHTD"
     gene            7184..7540
                     /gene="mnhG1"
                     /locus_tag="P6L01_000476"
     CDS             7184..7540
                     /gene="mnhG1"
                     /locus_tag="P6L01_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit G"
                     /protein_id="HDP3029570.1"
                     /translation="MIKIILISLALIFVIIGALISALAAIGLLRLEDVYSRAHAAGKA
                     STLGAMSLLFGTFLYFIATQGFVNMQLIVAIIFVLITGPLSSHMIMKAAYNIKTPYTK
                     KTKVDEISEDLKDTKL"
     gene            7789..8943
                     /locus_tag="P6L01_000477"
     CDS             7789..8943
                     /locus_tag="P6L01_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDP3029571.1"
                     /translation="MHWTIIGGGIQGTAIAQKLLSSGLTTDLLTIIDPHETFCQRFNS
                     YTNRIEMPYLRSPIVHHVHPQPFHLKQFAKQHQYTNAFYGPYQRPELTMFMNHIAHAS
                     KQYQLEDCLVQGLVQTLDKQEDKWHIKLEDGQIITTDCVVIAIGSTNIPFMPDILKDK
                     QNVNHIFEKEHDQVVYDKTDHIVGSGITAAHLALKLLNHDNDKKIHLWLNKDIEIHDF
                     DADPGWLGPKNMSSFLSTKSMPERNAIVQRERHKGSMPHELYLRLKKHIKNGRINVHK
                     TPITQISGGVINTENDSVPYQQIMVATGFEQDFMSQPLIKQLIQNYDAPINECNYPVI
                     SEKLEWIPNLFVAGCFADLELGPFGRNVMGGRKAAERIEQAFLKLQQYSA"
     gene            complement(8999..9373)
                     /locus_tag="P6L01_000478"
     CDS             complement(8999..9373)
                     /locus_tag="P6L01_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HDP3029572.1"
                     /translation="MTHLLETFEMSIDHQEDGLVVISMPVTDKVKQPFGYLHGGASIA
                     LGETACSLGSANLIDTTKFIPLGLEMNANHIHSAKDGRVTATAEIIHRGKSTHVWDIK
                     IKNDKEQLITVMRGTVAIKPLK"
     gene            complement(9392..10708)
                     /locus_tag="P6L01_000479"
     CDS             complement(9392..10708)
                     /locus_tag="P6L01_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HDP3029573.1"
                     /translation="MINAVVIAVILMIVLCLCRLNVVISLFISALVGGLISGMSIEKV
                     INVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVK
                     VTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPY
                     VLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPRE
                     YETRKISDSDNVTELKPYILIVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYN
                     WYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSII
                     MMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPA
                     SDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL"
     gene            complement(11119..12594)
                     /locus_tag="P6L01_000480"
     CDS             complement(11119..12594)
                     /locus_tag="P6L01_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HDP3029574.1"
                     /translation="MNFKLNNTLSNEINTLIIGIPEHLNQLERISFNHIDITESLERL
                     KHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKARSYQDMLKIWGHLFQYIKSEH
                     IEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESL
                     IELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVDVKDYDTL
                     VSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDVAPIALVGKGITYDSGGYSIKTKNGM
                     ATMKFDMCGAANVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGET
                     VEVMNTDAEGRLVLADAVYYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVI
                     LNDILQISSKVDEMVFELPITATERASINHSDIADLVNHTNGQGKALFAASFVTHFSG
                     QTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ"
     gene            complement(12725..13933)
                     /locus_tag="P6L01_000481"
     CDS             complement(12725..13933)
                     /locus_tag="P6L01_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDP3029575.1"
                     /translation="MAQDRKKVLVLGAGYAGLQTVTKLQKAISTEEAEITLINKNEYH
                     YEATWLHEASAGTLNYEDVLYPVESVLKKDKVNFVQAEVTKIDRDAKKVETNQGIYDF
                     DILVVALGFVSETFGIEGMKDHAFQIENVITARELSRHIEDKFANYAASKEKDDNDLS
                     ILVGGAGFTGVEFLGELTDRIPELCSKYGVDQNKVKITCVEAAPKMLPMFSEELVNHA
                     VSYLEDRGVEFKIATPIVACNEKGFVVEVDGEKQQLNAGTSVWAAGVRGSKLMEESFE
                     GVKRGRIVTKQDLTINGYDNIFVIGDCSAFIPAGEERPLPTTAQIAMQQGESVAKNIK
                     RILNGESTEEFEYVDRGTVCSLGSHDGVGMVFGKPIAGKKAAFMKKVIDTRAVFKIGG
                     IGLAFKKGKF"
     gene            complement(14387..14746)
                     /locus_tag="P6L01_000482"
     CDS             complement(14387..14746)
                     /locus_tag="P6L01_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HDP3029576.1"
                     /translation="MPTVILTEAAAYEVKDMLKANEMPDGYLKIKVNGGGCTGLTYGM
                     GAEEAPGENDEVLEYFGLKVLVDKEDAPVLNGTTIDFKQSLMGGGFQIDNPNAIASCG
                     CGSSFRTAKVAGNPENC"
     gene            complement(14759..14995)
                     /locus_tag="P6L01_000483"
     CDS             complement(14759..14995)
                     /locus_tag="P6L01_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzB family protein"
                     /protein_id="HDP3029577.1"
                     /translation="MNPIVEFCLSNMAKGGDYVFNQLENDPDVDVLEYGCLTHCGICS
                     AGLYALVNGDIVEGDSPEELLQNIYAHIKETWIF"
     gene            15312..16376
                     /locus_tag="P6L01_000484"
     CDS             15312..16376
                     /locus_tag="P6L01_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDP3029578.1"
                     /translation="MKNLVLLGGGYGNMRIMSRILTTSLPQDYTVTLVDRMPFHGLKP
                     EFYALAAGTKSDKDVRMKFPNHPQVNTVYGEINDIDLDAQIVSVGNSKIDYDELIIGL
                     GCEDKYHNVPGAEEYTHSIQTLSKARDTFHSISELPEGAKVGIVGAGLSGIELASELR
                     ESRSDLEIYLYDRGPRILRNFPEKLSKYVAKWFAKNNVTVVPNSNINKVEPGKIYNCD
                     EPKDIDLVVWTAGIQPVEVVRNLPIDINSNGRVIVNQYHQVPTYRNVYVVGDCADLPH
                     APSAQLAEVQGDQIADVLKKQWLNEPLPDKMPELKVQGIVGSLGDKQGFAYIMDRTVT
                     GRLASILKSGVLWLYKYHNG"
     gene            complement(16435..16758)
                     /locus_tag="P6L01_000485"
     CDS             complement(16435..16758)
                     /locus_tag="P6L01_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzD family protein"
                     /protein_id="HDP3029579.1"
                     /translation="MEHVSVVVYGADVICASCVNAPTSKDIYDWLQPLLKRKYPNISF
                     KYTYIDITKDNDNLTDHDLQFIERIEQDELFYPLITMNDEYVADGYIQTKQITRFIDQ
                     KLVNE"
     gene            16859..17101
                     /locus_tag="P6L01_000486"
     CDS             16859..17101
                     /locus_tag="P6L01_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HDP3029580.1"
                     /translation="MPTEDTTMFDQVAEVIERLRPFLLRDGGDCSLIDVEDGIVKLQL
                     HGACGTCPSSTITLKAGIERALHEEVPGVIEVEQVF"
     gene            complement(17364..18539)
                     /gene="dltD"
                     /locus_tag="P6L01_000487"
     CDS             complement(17364..18539)
                     /gene="dltD"
                     /locus_tag="P6L01_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDP3029581.1"
                     /translation="MKLKPFLPILISGAVFIVFLLLPASWFTGLVNEKTVEDNRTSLT
                     DQVLKGTLIQDKLYESNKYYPIYGSSELGKDDPFNPAIALNKHNANKKAFLLGAGGST
                     DLINAVELASQYDKLKGKKLTFIISPQWFTNHGLTNQNFDARMSQTQINQMFQQKNMS
                     TELKRRYAQRLLQFPHVHNKEYLKSYAKNPKETKDSYISGFKENQLIKIEAIKSLFAM
                     DKSPLEHVKPATKPDASWDEMKQKAVEIGKADTTSNKFGIRDQYWKLIQESKRKVRRD
                     YEFNVNSPEFQDLELLVKTMRAAGADVQYVSIPSNGVWYDHIGIDKERRQAVYKKIHS
                     TVVDNGGKIYDMTDKDYEKYVISDAVHIGWKGWVYMDEQIAKHMKGEPQPEVDKPKN"
     gene            complement(18536..18772)
                     /gene="dltC"
                     /locus_tag="P6L01_000488"
     CDS             complement(18536..18772)
                     /gene="dltC"
                     /locus_tag="P6L01_000488"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     2"
                     /protein_id="HDP3029582.1"
                     /translation="MEFREQVLNLLAEVAENDIVKENPDVEIFEEGIIDSFQTVGLLL
                     EIQNKLDIEVSIMDFDRDEWATPNKIVEALEELR"
     gene            complement(18790..20004)
                     /gene="dltB"
                     /locus_tag="P6L01_000489"
     CDS             complement(18790..20004)
                     /gene="dltB"
                     /locus_tag="P6L01_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG:teichoic acid D-alanyltransferase DltB"
                     /protein_id="HDP3029583.1"
                     /translation="MIPYGDFTFFLIALIALLPVIILGFLGKRSYIYNGVVTAFMIVL
                     IFSSDKHNLFDQKYLSVQLISFIIYVVWQVLLIMFYYHSKPKNNSFSKFVTVMVLSIL
                     PLALVKVLQSTWLGGHQIHFHESKLIEFVGFLGISYVTFKSVQLIMEIRDGSIKEIKV
                     WKLIQFISFFPTISSGPIDRYKRFVKDDKKVPTGNEYRELVLKAIHMIMLGFLYKYIV
                     AYFINTYAIMPLQLDLHGFVNLWLYMYAYSLYLFFDFAGYSLFAIAFSYLFGIKTPPN
                     FDKPFKAKNIKDFWNRWHMTLSFWFRDCIYMRSLFYMSRKKLLKSQFAMSNVAFLINF
                     FIMGIWHGIEVYYIVYGLYHAALFIGYGYYERWRKKHPPRWQNGFTTALSIVITFHFV
                     TFGFLIFSGKLI"
     gene            complement(20001..21458)
                     /gene="dltA"
                     /locus_tag="P6L01_000490"
     CDS             complement(20001..21458)
                     /gene="dltA"
                     /locus_tag="P6L01_000490"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDP3029584.1"
                     /translation="MTDIINKLQAFADANPQSIAVRHTTDELTYQQLMDESSKLAHRL
                     QGSKKPMILFGHMSPYMIVGMIGAIKAGCGYVPVDTSIPEDRIKMIINKVQPEFVFNT
                     TDESFESLEGEVFTIEDIKTSQDPVIFDSQIKDNDTVYTIFTSGSTGEPKGVQIEYAS
                     LVQFTEWMLELNKSGNKQQWLNQAPFSFDLSVMAIYPCLASGGTLNLVDKNMINKPKL
                     LNEMLTATPINIWVSTPSFMEMCLLLPTLNEEQYGSLNEFFFCGEILPHRAAKALVSR
                     FPSATIYNTYGPTEATVAVTSIQITQEILDQYPTLPVGVERLGARLSTTDDGELVIEG
                     QSVSLGYLKNDQKTAEVFNFDDGIRTYHTGDKAKFENGQWFIQGRIDFQIKLNGYRME
                     LEEIETQLRQSEFVKEAIVVPVYKNDKVIHLIGAIVPTTEVTDNAEMTKNIKNDLKSR
                     LPEYMIPRKFEWMEQLPLTSNGKIDRKKIAEVING"
     gene            complement(21474..21626)
                     /locus_tag="P6L01_000491"
     CDS             complement(21474..21626)
                     /locus_tag="P6L01_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDP3029585.1"
                     /translation="MKSKSKQPPNKYVEAFKPYLLTLLYLAIFITLYLIYGSGDTHNN
                     FIYNEF"
     gene            complement(21674..21784)
                     /locus_tag="P6L01_000492"
     CDS             complement(21674..21784)
                     /locus_tag="P6L01_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029586.1"
                     /translation="MMTTNYYVESIKLKLNFIMNIDIMNCKKQILKRILY"
     gene            complement(22038..22997)
                     /locus_tag="P6L01_000493"
     CDS             complement(22038..22997)
                     /locus_tag="P6L01_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HDP3029587.1"
                     /translation="MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALR
                     DADACFITLSEQIDAEILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTE
                     TTAELGFTLMLAIARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKA
                     FARRLQGFNTNILYHNRSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKF
                     NAEAFEQMKNDAIFINIGRGQIVDETALIDALDNKEILACGLDVLANEPIDHTHPLMG
                     RDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHTPVN"
     gene            complement(23030..23809)
                     /locus_tag="P6L01_000494"
     CDS             complement(23030..23809)
                     /locus_tag="P6L01_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDP3029588.1"
                     /translation="MKQYKAYLIDLDGTMYMGTDEIDGAKQFIDYLNVKGIPHLYVTN
                     NSTKTPEQVTEKLREMHIDAKPEEVVTSALATADYISEQSPGASVYMLGGSGLNTALT
                     EAGLVIKNDEHVDYVVIGLDEQVTYEKLAIATLGVRNGATFISTNPDVSIPKERGLLP
                     GNGAITSVVSVSTGVSPQFIGKPEPIIMVKALEILGLDKSEVAMVGDLYDTDIMSGIN
                     VGMDTIHVQTGVSTLEDVQNKNVPPTYSFKDLNEAIAELEK"
     gene            complement(23809..24243)
                     /locus_tag="P6L01_000495"
     CDS             complement(23809..24243)
                     /locus_tag="P6L01_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HDP3029589.1"
                     /translation="MYFVDKDKLTQKLAYLQALTDDYHESKHNHYAFERIAQMLIESS
                     VDIGNMIIDAFILRDPGNYKDVIDILELENVITKETQQEINKTVDIRKQFTYDYTALD
                     IKVIMPMFDDALPYYKQFITEVTIFLHQENVPVTAFGKGENQ"
     gene            24341..24607
                     /locus_tag="P6L01_000496"
     CDS             24341..24607
                     /locus_tag="P6L01_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3055 domain-containing protein"
                     /protein_id="HDP3029590.1"
                     /translation="MIDMYLYDDNEESQVQFVGFVGEHSRYDLMLVHTNRHYGKTLVL
                     NMQTNKFGIIGTDDLKEEGYIAHILGVNAEEGDEITEYLNEVIH"
     gene            complement(24681..26000)
                     /locus_tag="P6L01_000497"
     CDS             complement(24681..26000)
                     /locus_tag="P6L01_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1181 family transposase"
                     /protein_id="HDP3029591.1"
                     /translation="MCNDTLELLRIKDENIKYINQEIDVIIKGKKATVVNAVLTYKPS
                     ACYCCGVKNEGQIHKHGKRVSRITLLKTQGYNTYLNLAKQRFKCLECNGTFTAKTSIV
                     DESCFISRCVTQKVIEEATKVKTEIDTAEDNCISPSTVSRIRTKAANSLRIKPFNCLP
                     EHIAMDEFKSVKNVTGSMSFIFIDNDTHDVIDILENRTTRFLRAYFERFDLKNRQQVK
                     TVTIDMYEPYVRLFRDLFPNAAIIFDRFHIVQHLNRELNKYRVQVMNEYRNKKGPNYT
                     IFKNNWKVLLMDTSKTIFSKYRWNKSFKAYKRSSDIVEFMLSKDDILRRSYELVQGLR
                     KDLRLCNWPKFINRLNSVSKKSVSKGVWKVVKYYRKHQRMLRNTIYYPAFNNGAIEGI
                     NNKIKLIKRISFGYRNFNNFKARIMMIFSLYKGEKKKTTKPNNGLAA"
BASE COUNT         8324 a         4530 c         3936 g         9280 t
ORIGIN      
        1 tagacaaaat tttagcttta acaatttggc cttctgatag aaatttcttc aaattatgaa
       61 cgtagtcatc cataatttct gatatatgaa tcagtccttc agtatgatta ggggtctcaa
      121 caaacgcacc gtatggttga ataccagtta cacgcacctt gatatgttgg ccaattttgt
      181 agttattcaa cttaataacc ccttatttcg tttacttaac actattatca tagcacaaat
      241 cgaaatggtg gactaattaa aacatcaaat catacgaata ttattttata aatgaataag
      301 aattctacaa acttggattt acaaataaaa ttagcaacaa actcctatct aaatcaatat
      361 gatatccagc tactttgttt tcaatttatt aactatttga atagcaattc tctttaaaaa
      421 cataaaaagc aacctcaact attatcttta gaatacacat ctatttaaaa actgtgcttt
      481 cttgataaat atattgagat tgctcatcat gttagtttat ttgtttcgtt ggaatatata
      541 ctaatccatt cataaattag ctttatccga gtttaaaatg ttggtagtta attatgttta
      601 gctattattc ttcaacatca atagattcaa ttacaacatc atgaagtggt ttgtcttgtg
      661 gtcccacttt tgtatttgca atatcttcta aagtagtttc accatcaatg atttgaccga
      721 atactgtatg tttttgatct aaccatggtg taccaccctt ttcgccatat gcatcaacga
      781 ttggttgagg ccagccacca tctgcaagtt gacttaacat attttgaggt acttctttca
      841 tttgaacaat gaaaaattgt gaaccattag tattaggtcc tgagttagcc attgataatg
      901 cgccatataa gttaaatgca tttaatgaaa attcatcttc aaaagcaccg ccataaatac
      961 tttcgccacc cataccagta gctgttggat cgccaccttg aatcatgaag tcattaatga
     1021 cacggtggaa tgttattcca tcataataac cattttttgc atgtgtcaca aaattttcaa
     1081 ctgtttttgg tgcaatattt ggaaataatt tgaatgtcat gtcaccttta tttgtgtgca
     1141 taaccacttt aatttcacct tgttgtactt ctttgtttaa ctgtggatag ttagccattt
     1201 atattctcca ttcatgttat gatacatata tattaacata cttttaaggg aggaaaatag
     1261 catgaagcta tatcgatttt cacacaaaac aggtagctat ggtgtcacga ttgttgaaga
     1321 aaatggagac gatgtccttg ttaaagttga acaagttatc aaacacccga aacaaggtga
     1381 tttacataac cctactgaaa ctgaaggtgt atttttccat gaacgcaaag cattaagcca
     1441 ttttgaaaaa cgctatacga aacgttcaca attacgcgac ttcaatgttg atgtattgcg
     1501 ctatgaagat tcacttcaac aagcaattac agatttagaa actaaactta aagctcagca
     1561 aacaaaacat gctgaaatgt ctttagctag tttagcacgc ttaaaagaag attattcact
     1621 tcaatacaaa gaaaatttta aataaaaaat gacgtgtttc ttcttattta aatacttctg
     1681 aaaataaagc acaacaagga gtgtgtacgt ttttcagaac tatatcattc atgtataatg
     1741 aataacataa atatttttta aaggaggaca acatagtgag tttgttacat attgcggtga
     1801 tattaccgtt aatattcgca ctcattattc ctatcctgta tagatttttt aaacgtatac
     1861 atttaggatg gttcgtatta cctgtcccta tcgtcatatt tatttatatg cttacattaa
     1921 ttaaaacgac gatgtcaggt aatactgtaa tgaaaacctt aaattggatg cctcattttg
     1981 gtatgaactt tgatttatat cttgatggct taggtttact ctttagtttg cttatttcag
     2041 gaatcggcag tttagtagta ttatattcaa tcggctactt aagtaagtca gaacaactag
     2101 ggaattttta ttgttattta ttactattta tgggtgcaat gttaggtgtc gtactttcag
     2161 ataatgttat tattctatat ttattctggg agctaacgtc attttctagt tttttactta
     2221 tttcattctg gcgcgaacga caagcatcta tatatggtgc tcaaaaatcg ctcataatca
     2281 cagtatttgg tggtctatca ttattaggtg gtatcatctt actcgctata ccaacacaat
     2341 catttagcat tcagtacatg attcaacatg cttctgaaat tcaaaatagc ccattcttca
     2401 tttttgcgat gattttaatc atgattggtg cttttacaaa atctgctcag tttccatttt
     2461 atatttggct accagatgca atggaagcac ctacaccagt cagtgcttat cttcattcag
     2521 caacaatggt taaagcaggg ttatatttaa tcgctcgaat gacaccaatc ttcgcagcat
     2581 cgcaaggttg ggtatggact gtcacactcg ttggtttaat tacgttattc tgggcttcgc
     2641 taaacgcaac aaaacaacaa gacttgaaag gtatactggc attttcaaca gtatcccaat
     2701 taggtatgat tatggcgatg cttggaatcg gtgccattag ttatcactat caaggcgatg
     2761 atagcaaaat atatgctgct gcatttacgg ctgcaatatt ccatttaatc aaccatgcaa
     2821 catttaaagg tgccctattt atgattacag gtgcagtgga tcattctact ggtacacgtg
     2881 atgttaagaa attaggtggc ttactaacaa ttatgcctat ttcatttaca atcactgtta
     2941 ttacagcatt aagtatggct ggtgtaccac cgttcaatgg tttcttatca aaagaatcat
     3001 ttttagaaac aacatttaca gcaagccaag caaatttatt cagtgttgat acattaggct
     3061 acctattccc aatcattggt atagtgggca gtgtcttcac atttgtatat tcaattaaat
     3121 ttataatgca tatatttttc ggacaatata aacctgaaca attacctaaa aaagcacacg
     3181 aagtatcaat attaatgctt ttatcaccag ctattttagc tacattagta attgtattgg
     3241 gtttattccc tggcatttta actaattcga ttattgaacc agctacatct tcgattaatc
     3301 atacggtaat tgatgatgtt gaattccata tgttccatgg attaacgcct gcctttttat
     3361 caacattagt gatttatatt ttaggtatct tactaattgt gacatttagt tattgggtga
     3421 aattgttgca acgacaacct ggtaaactaa cattcaatta ttggtacaac agaagtgcaa
     3481 atgttattcc aaactactct gaaaagatga cgaatagcta tgttaccgat tattcacgta
     3541 ataatttagt tatcattttt ggtgcattaa ttttattaac atttgtcact atattctcag
     3601 tgcctttcaa cattaatttt aaagacgtta gtccgattag aattttcgaa gtttgtatcg
     3661 taattttatt attaagtgct gcctttttaa ttcttttcgc taaatcaaga ttatttagca
     3721 ttattatgtt aagtgcagtt ggatatgctg tgtctgtatt gtttatattc tttaaagcac
     3781 ccgacttagc attgacacag tttgtcgttg aatctatttc aacagcattg ttcttattat
     3841 gcttctacca cttaccaaat ttaaatcgtt ataatgaaaa gcgctcattc cagcttacta
     3901 atgctttaat tgctggtggt gttggtttgt ccgtcattat tatcggttta attgcatacg
     3961 gaaatagaca ttttgaatcg atatctaagt tttatcaaga gcacgtttat gatttagctc
     4021 acggtaaaaa catggtaaac gtcatacttg ttgatttccg tggtatggat actttattcg
     4081 agtcatctgt gcttggtatc gcaggattag ctgtatatac aatgattaaa ttacgtaaaa
     4141 agcgtcaaac gcaaggtaac gaggtgaaaa atcatgaata gacaacaaaa tgatctaatt
     4201 ttgcaatttg ctgcagttat tatctttttc atggtaatgg tctttggttt ttcacttttc
     4261 ttagcaggac attatacacc tggtggcggt ttcgtcggcg gattattatt cgcaagttca
     4321 cttgttatca ttacgattgc atttgatata gaaacaatgc gtaaaatttt cccattagat
     4381 tttaaaatct tgataggtat cggattagtt ttttgtattg cgacgcctat cgcaagttgg
     4441 tttttaggca aaaatttctt tacgcatgtc acatttgata taccgttatt tatattagaa
     4501 cctgttcata tgacaacagc agtattcttt gatttcggtg ttttatgtgc agtagttggt
     4561 actgtcatga caattattat ttcgattgga gagaacgaat agtggaaatt attatgattt
     4621 ttgttagtgg tattctcaca gcaattagtg tctatctcgt tttgtctaaa agtctgatac
     4681 gaattgttat gggaactaca ctattaacac atgcagcaaa cttattttta ataactatgg
     4741 gcggacttaa acatggtact gttccaattt atgaagcgaa cgtaaaaagc tatgttgatc
     4801 ctatcccgca agcacttatt ttaacagcaa tcgttatcgc ctttgcgaca acagcctttt
     4861 tcttagtatt agcatttaga acatataaag aattaggcac agataacgtt gagagtatga
     4921 aaggagttcc agaagatgat tgaaagtaat atgcttgtgt taacgctagt tattccagta
     4981 attacagcca tattacttgt ctttattgga aagcgtccta ttataaagcg ttatgtcgca
     5041 ctaggcggta cattattaac cttggtcgcg gcaattatca atttagcaaa tgttgttaaa
     5101 catggaccaa tacgcgtaga gctaggttca tggaaagcac catacagtat tgtctttgta
     5161 ttagacatat tcagttccct actaataatt actagtatta ttattacagc tattgtgatt
     5221 ttatattcat accaaacaat tggtattgaa cgtgaaagat attattatta tttttcagta
     5281 ctatttatgt taataggtat cattggtgca ttcacaactg gtgacatttt caacctattc
     5341 gtatttttcg aagtattttt aatgtcatct tatttcttat tagtaattgg ttctacaaaa
     5401 attcaactac aagaaacaat caaatatgtt cttgttaacg ttgtatcgtc atccttcttc
     5461 gtaatgggtg tagcgatttt atattcagta gtaggaacat tgaatctagc agatataagc
     5521 aataaattag ctaacctttc agcgcatgac agtggcttag tcaatatcgt tttcatacta
     5581 tttatctttg tttttgctac aaaagctggc gtattcccaa tgtttgtttg gttacctagt
     5641 gcatactatg caccaccaat tccaatcatt gcattctttg gtgctttgtt aacaaaagtt
     5701 ggtgtttatg caatcgctag aacattaagc ctattcttta gtgataatgt aagcttttca
     5761 cattatgtca ttttattctt agcactatta acgatcatct ttggttgtgt cggtgctgtt
     5821 gcatatgcca acattaaaaa aatcatccta tacaatgtca tgattgctgt aggtgtgatc
     5881 ttagtcggcg ttgcaatgat gacagagtca ggtatgattg gggctattta ctatacattg
     5941 cacgacatgt tagtaaaact tgcattattc ttgcttatcg gaattatgat taaaattaca
     6001 ggcactgctg atttaagaca atttggtggc ttaattaaac gatatcctgt acttggttgg
     6061 tcattcttta tcgcagcatt aagtttagct ggcattcccc cactcagtgg cttttatgga
     6121 aaatttttca ttgtccaatc aacatttgaa agaggatttt atttaagtgg tgtcatcgtc
     6181 ttattatcaa gtttagtcgt attatattct gtcattcgca tattcctaca aggctttttc
     6241 ggtcaaccaa aaggatatga tcttaataac aaagtcgacg tcaaatattt aactacgatt
     6301 gcaatcgttg cagttgtgat aactgtttta tatggattgt cagcagatta cttatatcca
     6361 atggtaaaag ctggtgcaga aacattctac aatccatcaa cctacgttaa agctgtatta
     6421 ggaggtaaat aacatggctg tacaattagt attaaacttt attatcgcag tgttttggtt
     6481 gtttgtaaca aatagttaca caacaaataa ctttgtgtta ggtttcatct ttggcctagt
     6541 tcttgtttac ctcttacaca gagtgttacc aggtagattt tatgtcatca cgctatatcg
     6601 tattattaaa ttagttatta ttttcttaat cgaactgatt aaagctaatt ttgatgtttt
     6661 gaaaattatt attaaacctt cgattaaaaa tgaaccagga ttctttgttt atcacacaga
     6721 tttaaaaaaa gattggcaaa ttgtcttgct gtctaattta attacattaa cacctggtac
     6781 tgtagtgctt ggtgtaagtg atgatcgtac aaaaatttat attcatgcca tagatttttc
     6841 aactaaagag caagaagtag aaagtattaa aacatcatta gagaaaattg taagagaggt
     6901 gggcgaaata taatgaatca taatgttatt atcgttattg cattaatcat agttgtcatt
     6961 tctatgttag ctatgctcat tcgcgttgtg ctaggcccat cacttgccga tcgtgttgtc
     7021 gcattagatg cgattggtct tcaattaatg gcagttatag cattattcag tattttatta
     7081 aatattaaat acatgattgt cgttattatg atgattggta tattagcctt tttaggtact
     7141 gcagtattct ctaaatttat ggacaaaggt aaggtgattg aacatgatca aaatcatact
     7201 gattagtctt gcacttatct ttgttatcat cggtgcttta attagcgccc tagcagctat
     7261 aggattattg agacttgaag atgtatattc acgtgcacat gctgccggaa aagcatcaac
     7321 attaggtgca atgtcattac tatttggtac ttttttatat tttattgcta ctcaaggttt
     7381 tgtaaatatg caattaatcg ttgcgattat ctttgtatta attacaggtc ctctttcaag
     7441 tcatatgatt atgaaagcag catataatat taaaacgcct tatactaaaa agactaaagt
     7501 cgatgaaatt tcggaagact taaaagacac aaaattgtag attatataac aaaatcatga
     7561 atataaatca acaacaaaca gcagtaagat gattccaaat taggaatgat tttactgctg
     7621 ttttcttttg acattgttac ctctttttca atgatttttt ctttgactac atcttcgccc
     7681 tatctacgta tatctcttta atttaattgc ctttcatgtc gttatgtatt atgataataa
     7741 taatcataaa tcgtaacgat tacgctttaa aaaaagagag gttttattat gcattggaca
     7801 attatcggcg gtggcataca gggaactgca atcgcacaaa aactattatc aagcggatta
     7861 acaacagacc tattaacaat cattgaccca cacgaaactt tttgccaaag gtttaactca
     7921 tatacaaatc gaatagaaat gccttattta agatcaccga ttgtacatca cgtacatcca
     7981 caaccgttcc atctaaaaca attcgctaaa cagcaccaat atacaaatgc tttttatggt
     8041 ccttatcaac gacctgaatt gacaatgttt atgaatcata ttgcacatgc ttctaaacaa
     8101 tatcaattag aggattgctt ggttcaaggt ttagttcaaa ctttagataa acaagaagac
     8161 aaatggcata tcaagttaga agatggacaa attatcacta cagattgcgt cgttattgca
     8221 ataggtagta caaatattcc gtttatgcct gacattttaa aagacaaaca gaatgtaaat
     8281 catatcttcg agaaagaaca tgatcaagta gtatacgata agaccgatca tatcgttggt
     8341 agtggcatta ctgctgcaca tcttgcactt aaattgttaa atcatgataa cgataaaaag
     8401 attcatttat ggctaaataa agatattgaa atacatgact ttgatgctga tcctggttgg
     8461 ttaggtccga aaaatatgtc ttcattttta agtactaaaa gcatgcctga aagaaatgcc
     8521 attgtacaac gtgaacgtca taaaggatca atgcctcacg aactgtactt acgccttaaa
     8581 aaacatatta aaaatggtcg tataaatgtg cataaaacac ctatcactca aattagtggt
     8641 ggtgtaatta acactgaaaa tgattctgtt ccatatcaac agattatggt tgcaactggt
     8701 tttgaacaag attttatgtc acaaccactt attaagcaat taatacaaaa ttatgatgca
     8761 cctatcaacg aatgtaatta ccctgttatt tccgaaaaat tagaatggat accaaatcta
     8821 tttgtcgcag gatgctttgc agacttagaa ttaggaccat ttggtagaaa tgttatgggt
     8881 ggccgtaaag ctgccgaacg cattgaacaa gcatttctaa aattacaaca atatagcgca
     8941 taaaaaaact gctagccgta gttatgaata tctcataaca tttcagctag cagttctttt
     9001 attttaaagg tttaatagca actgtaccac gcataactgt aattaattgt tctttgtcat
     9061 tcttaatttt tatatcccat acatgtgtcg acttacctcg atgaataatt tcagctgtcg
     9121 cagtaacacg accatcttta gcagaatgaa tatggttagc attcatctct aaacctaatg
     9181 gaataaattt ggttgtatca attaaattag cagatcctaa tgaacatgct gtttcaccta
     9241 aagcaatcga agccccacca tgtaaatatc caaatggttg ttttacttta tcagtgacag
     9301 gcatagaaat aacaactaaa ccatcttcct ggtgatctat tgacatctca aatgtctcta
     9361 ataaatgagt catattttca cctctttatg cttatagtac catagcagca atagtaccga
     9421 aaatcattaa aggaatatta taaaacaaga agtttggtac acatgtatcg cgtatatgat
     9481 catgttggcc atcaacattt aatcccgcag ttggtcctaa tgttgaatca cttgcaggtg
     9541 aacctgagtc acctaatgca ctcgctgttc caatcaatgc gattaatgcc attgtatcta
     9601 gtccaattga cgctccaaaa ggaatgaata atgatgcgat aataggaatt gttgcaaatg
     9661 atgatccaat acctaaagtg acaattaaac ctatcacata catcatgata atgctaaata
     9721 atttattatc accagtaata cttgttaaag ttttaactaa ttcatctata tcaccagtag
     9781 cattcattac accagcaaac ccatttgctg ttaaaataac tacaccaata taagccataa
     9841 ttttaatacc ttcaacaaac ttagcatcta attcatacca attatatgca cgtgaaataa
     9901 agaatacgag tacccctgcc agtgcaccaa aaatcattga atctgtaaat gtttgtacta
     9961 aaaatgtagc tagtattgcc acaattgtta ctattaagat atatggtttt aactctgtaa
    10021 cattgtcact atctgaaatt ttacgtgttt catattcacg tggtttacga tatacatata
    10081 aaccgataag taagccaaca atatacccca ttgaaggaat aagcattgct ttccaaatca
    10141 tattaaactc aattgggtga ttcgcctttg caaagccact ttgaataatt tgctggaaaa
    10201 tttgaccgaa tccatatggt aataacacat acgggaaaca taaaccaaaa ccgataatca
    10261 aaccgattaa acgtctatct atttttaagt cattaaacag acttaacaat ggtgggatga
    10321 caattggaat gaatgcaata tgtacaggaa ttaagttttg actcatgata ctcatagcta
    10381 ataatgcaat gattattgtc acttttactt taactcttga ccatcgacta ttttcagcgt
    10441 gaattgcatt tataattttt cctactaaat agtctgtgat accactgtat gaaattaatg
    10501 ctgcaaatcc acctaataat gcatagctta atgctacctc agcaccatcg actatatttt
    10561 tcccaaatac atttataact ttttcaatgc tcatgcctga aattaagcca ccaactagcg
    10621 cactgataaa taagcttata actacgttta atcgacataa acatagcaca atcattaata
    10681 ttactgctat tactactgca tttatcatat gataaccatc ctttatcgct ctatcgaact
    10741 aaagcgttta tagactacat agtatcagta gctttaacac ctgtcaatag caaactatac
    10801 ccataatctt tattttttag ttaattgtct ttcttaaaat aattttcact ttcattaaat
    10861 taaacaattt tacaagcctg gaacaccaat caaaatccta agttctataa tgttatatta
    10921 gtagtcgttg actgaatgag catatgatta taataagttt ttgcaatgct aatcaagttg
    10981 atttatgctc acaaggatat gcgatttata ttttcttaca acaatgaaaa tgcctgatac
    11041 aatgcgatcc tttatacttt caagagttaa attagaaatg tataacaaga cacaagttgt
    11101 atcaggcaaa taattaaatt attgttgttt taaccattgt actatcgtcg gaatcataaa
    11161 tcctgttggc ccttttggac cattatatga agctttatta gtcgttgctg gacctgcaat
    11221 atcgaaatga atgtgaggtg tttgaccact aaaatgtgtt acaaaacttg ccgcaaatag
    11281 cgctttacct tgtccattcg tatggttaac taaatcagcg atatcactgt gattaatact
    11341 tgcacgttcg gttgcagtaa tcggtaattc aaataccatt tcatcgactt tagaacttat
    11401 ttgtaatata tcgtttaata tcactttact attcgattca aatgcagcag ctttatcatc
    11461 gcctagtgca acgatggctg cacccgttaa tgtagcaaag tccataatca cactaggctg
    11521 atattgattt gcataataca cagcatctgc aaggactaat ctaccttcag cgtctgtatt
    11581 cattacttct acagtttcac cacttaatgc tgtaaataca tcatctggct tcattgatgc
    11641 ttcatttatc atattttcag cacaagcaag cactccgaca atatttacag gcagttgtaa
    11701 acgactagcc gcttcaatga taccaacgac attcgcagcg ccacacatgt caaacttcat
    11761 tgtagccatg ccattcttcg ttttaatact ataaccacca gaatcatacg ttataccttt
    11821 accaactaag gcaattggtg ctacatcttt gtctttgcca ttatatgtga tggttactaa
    11881 tctcggttta tgcttactac ctttacctac tgcttgtaaa agtccgaatc cttcagaaac
    11941 taaagtgtca taatctttaa catctacttt gacctttgta tttttaaaat gattaacaat
    12001 atcttctgca aatgtttgtg gtgttaatac attcggtggc atattactaa agtctcttgc
    12061 caaattaatg gattggccaa tactgatacc ctcatgaatg aaatctaatt caattaatga
    12121 ttcactaata aggtttaaat tcgtcttaaa cggtgccttc ttacttgatt tataatgatc
    12181 gaattcatat gttgcacgct cactttgaat accgcatgcc attaatacat ctgataactg
    12241 atcatatttt gaaataaatg aatccataag taaataagta tcttcaatat gttctgactt
    12301 tatgtattgg aaaagatgtc cccatatttt caacatatct tgataactac gtgcttttaa
    12361 gtttcctaaa ccaactgtaa ttaaacgata tgtttgatct tgtacatcaa atgcagttgt
    12421 ataaatcttc ccaactttac taccaataat atgttgatgt tttagtcttt caagtgattc
    12481 tgtaatatcg atatgattaa aactaatgcg ctctaactga tttaaatgtt ctggtatacc
    12541 aataatcaat gtatttattt cgttgcttag tgtgttattt aatttaaaat tcataatgta
    12601 cctccttaga tttgatatgt tactcacttc aaattgtaca acaataaagc ccttcagtga
    12661 cactgaagga ctttataaat gaataattta attttatgtg tttagctgaa actttattta
    12721 aagcctagaa tttacctttt ttgaatgcta aaccgatacc accgattttg aataccgcac
    12781 gtgtatcaat cacttttttc atgaatgctg cttttttacc agcgataggt ttaccaaata
    12841 ccatacctac accgtcatgt gaacctaaag aacaaacagt tccacgatca acatattcga
    12901 attcttcagt tgattcaccg tttaagatgc gtttaatgtt tttagcaaca ctttcacctt
    12961 gttgcattgc aatttgtgct gtagttggta atggacgttc ttctccagct gggataaacg
    13021 ctgaacagtc accaataaca aaaatgttgt cgtaaccatt gattgttaaa tcttgctttg
    13081 taacgatacg tccacgttta acgccttcaa atgattcttc cattaattta ctaccacgta
    13141 caccagctgc ccatactgaa gtacctgcat ttaattgttg tttttcacca tctacttcaa
    13201 ctacaaaacc tttttcgtta caagcaacga ttggtgtagc aattttaaat tcaacaccgc
    13261 ggtcttctaa gtagctaact gcgtggttaa ctaattcttc tgagaacatt ggtaacattt
    13321 taggtgctgc ttcaacacaa gtgattttaa ctttattttg atccacacca tatttgctac
    13381 ataattcagg aattctgtct gttaattcac ctaagaattc aacaccagtg aatccagcac
    13441 caccaactaa gatagataaa tcgttatcat ctttttcttt tgatgctgca tagttagcaa
    13501 atttgtcttc gatatgacgt gataattcac gtgctgtgat aacattttca atttggaaag
    13561 catgatcttt cataccttcg atgccgaatg tttcactaac gaaacctaat gctactacta
    13621 aaatatcaaa gtcataaata ccttgatttg tttctacctt tttagcatca cggtcaattt
    13681 ttgttacttc tgcttgaaca aagttcactt tgtctttctt caagacactt tccacaggat
    13741 ataatacatc ttcatagttt agtgtacctg ctgatgcttc atgtaaccat gttgcttcat
    13801 agtgatattc atttttatta ataagcgtaa tttctgcttc ttctgttgat atcgcttttt
    13861 gcaatttagt tacagtttgt aaacctgcat aaccagcacc aagtacaagt acttttttac
    13921 gatcttgagc catttaattt cacctaagct ttcatatttt tttaaccaaa tgctgataat
    13981 tactagtaac aactggtcat tactactttt accacaatgt atactagttt atcatcattt
    14041 tgttatttaa gtcttttaaa ttatgtaaaa acctgtgatg ttcacaaaaa agacattttt
    14101 aatccaatac taattctata gcttttatgt tatattttca agcaagattc agtcagattg
    14161 tgaaaaaatt aattaatttt ttcgtagcct gatttcgatt aaaaatgtat ccgttatcac
    14221 gatcatttaa attactattt atagtcagat tcttaatttt caataagctt tttaataaaa
    14281 caaaatatct ttacgtaaaa ataggacata atcaaatatg tccctaatca taattgatat
    14341 caatttgtat cccaaaaatt gggttaacca ttaaaaagtt attttattag caattttcag
    14401 gattacctgc aacttttgca gttctaaatg aactaccaca gccacatgaa gcaattgcat
    14461 taggattgtc gatttggaaa ccgccaccca ttaatgattg cttaaaatca atagtcgtac
    14521 catttaatac gggtgcatct tctttgtcta ctaatacttt taatccaaag tattctaaga
    14581 cttcatcatt ttcaccaggc gcttcttctg cacccatacc gtatgttaaa ccagtgcacc
    14641 cgccaccatt cactttaatt tttaaatagc catctggcat ttcatttgct ttaagcatat
    14701 cttttacttc gtaggcagct gcttctgtta atataactgt tggcatgata actcctcctt
    14761 aaaaaatcca tgtttctttt atatgtgcat atatattttg taataattct tccggcgaat
    14821 caccttcaac aatatcacca tttactaaag catacaaccc ggctgaacat ataccacaat
    14881 gtgtcaggca accatactct aacacatcga catctgggtc attttccagt tgattaaaaa
    14941 cataatctcc accttttgcc atgttagaga gacaaaattc tacgatcgga ttcatacttc
    15001 accttcttat ttcatttgtt acaatattat agcattttaa aactggtatt ttaacatgat
    15061 gtgctcaatt accaacaact gatgtttctt atcccagtta tgtaatagtg ccttagttag
    15121 tacaaatatt tatttgtagt cattttcaaa aaaatatata ataagcatat aaatacatat
    15181 ttactttttt aggggttatt gatgaatcaa agacacttgc attttataac tgttcattaa
    15241 caaatatatc tatgcacttt gatcttttca aaattaaagt aaatattaac atataaaagg
    15301 ggtacttcat tatgaaaaac ttagttttgt taggcggcgg atatggtaat atgcgtatca
    15361 tgtcacgcat tttaactact tctttaccac aagattatac agtcacatta gttgatcgta
    15421 tgccatttca tggattgaaa ccagaattct atgctttagc tgcgggcacg aaatcagata
    15481 aagatgttcg catgaaattc cctaatcatc cacaagtgaa tacagtttat ggtgaaatta
    15541 acgacataga tttagatgct caaattgtct cagtcggtaa ttctaaaatt gattatgatg
    15601 agctaatcat tggtttagga tgtgaagata agtatcataa cgttccagga gccgaagaat
    15661 atacacatag tattcaaaca ctctcaaagg ctcgggatac tttccatagt attagtgaac
    15721 taccagaagg tgctaaagtt ggtatcgttg gtgctggatt aagtggcata gaacttgcca
    15781 gcgaattaag agaaagtaga tcagacttgg aaatatatct ttatgaccgt gggccgcgaa
    15841 ttttaagaaa ttttccagaa aaattaagta agtatgttgc gaaatggttc gccaaaaata
    15901 atgttaccgt tgttccaaat tcaaatatta ataaagttga acctggtaaa atatataact
    15961 gtgatgaacc taaagatatt gatttagttg tatggacagc aggaattcaa cctgttgaag
    16021 ttgttcgtaa cttgccgatt gatataaata gtaatggacg cgtgatagtt aaccagtatc
    16081 atcaagtacc aacatatcgt aacgtctatg tagttggtga ttgtgctgat ttaccacatg
    16141 cgccaagtgc tcagttagcc gaagttcaag gtgatcaaat tgccgatgtg cttaaaaagc
    16201 aatggctaaa tgaaccatta cctgacaaaa tgccggaact aaaggtacaa ggtatcgttg
    16261 ggtcattagg agataaacaa ggttttgcat atatcatgga ccgcactgta acaggacgac
    16321 tcgcttctat tttaaaatcc ggcgtacttt ggttatataa atatcataat ggttaaaatt
    16381 aaaaagcaat gtcagttcac actattactg acattgcttt ttgttatgtg tatattattc
    16441 attcacaagc ttttggtcta taaatcgagt gatttgcttt gtttgtatat aaccatctgc
    16501 tacatattca tcgttcattg taattaatgg ataaaatagt tcatcttgtt ctattctttc
    16561 aataaattgt aaatcatgat ctgttaagtt gtcattatct tttgtaatat ctatatacgt
    16621 atatttaaat gatatatttg ggtattttct ttttaatagc ggctgtagcc agtcataaat
    16681 atctttcgat gttggcgcat taacgcaact tgcacatata acatctgccc catatactac
    16741 cacactcacg tgctccatat atttatcccc cattggtttg atagattttt attacactat
    16801 ctattataat atactaataa agattatcaa attcatctct cgaaaggaga cttgcctgat
    16861 gcctactgaa gatacaacga tgtttgatca agtagcagaa gttattgaac gtcttcgtcc
    16921 atttttatta cgtgatggtg gcgactgctc attgattgac gttgaagacg gtattgttaa
    16981 attacaatta catggtgcat gtggtacatg cccaagttct acaatcactc ttaaagctgg
    17041 tattgagcgt gcattacacg aagaagtgcc tggtgtaata gaagtagaac aagtattcta
    17101 atataaagat attaagttaa cagaacatat tatgaataac aacaaagaca ggaatactga
    17161 attatttata atcagttatt acctgtcttt tttcgctgtc aaattggact gatgggaaag
    17221 ttattttata ctatttatct gattaattat tttattattg ccacatatcc aagcacatat
    17281 tactattcta ttctatgaaa tttaaaatat aaaaatagca catacgctca atcaaaatcg
    17341 ttgagttatg tgctatttgt attttaattt ttaggtttat ctacttcagg ttgtggttca
    17401 cctttcatat gtttcgcaat ttgctcatcc atataaaccc aacctttcca accgatgtgt
    17461 acggcatcac tgataacata tttttcataa tctttatcag tcatatcgta aattttacca
    17521 ccattatcta caacagtaga atggattttt ttataaactg cttgacgacg ttctttatcg
    17581 ataccaatgt ggtcatacca tacaccgttt gatggaatac ttacatattg aacatctgca
    17641 ccagcagcac gcattgtttt tacaagtaat tctaaatctt ggaattctgg agaattaaca
    17701 ttgaattcgt agtcacgtct aactttacgc ttactttctt gaattaattt ccagtattga
    17761 tctctaatac caaatttatt cgatgtagta tcagctttac caatttcaac tgctttttgt
    17821 ttcatctcat cccaagaagc gtctggtttt gtagcaggtt taacatgttc taatggagat
    17881 ttatccattg caaacaatga tttaatcgct tctattttaa tcaattgatt ctctttaaaa
    17941 ccagaaatat aactatcttt agtttcttta gggtttttag cataagattt caagtattct
    18001 ttattgtgta catgtggaaa ctgtaataaa cgttgtgcat aacgacgttt taattcagta
    18061 gacatgtttt tctgctggaa catttgatta atttgagttt gagacatacg agcatcaaag
    18121 ttttgattcg ttaaaccatg gtttgtaaac cattgtggtg aaataataaa tgttaatttc
    18181 ttacctttta atttatcata ctgtgatgca agttcaactg cgttaattaa gtctgtagaa
    18241 ccaccagcac ctaataagaa tgcttttttg ttggcgttat gcttatttaa tgcaattgca
    18301 ggattaaatg ggtcatcttt acctaattca ctagagccgt atataggata atacttgttt
    18361 gattcgtata acttatcttg aatgagtgtg ccttttagta cttgatctgt caatgaagtt
    18421 ctattatctt ctacagtctt ttcatttact aatcctgtaa accaactagc aggtaataat
    18481 agaaagacaa tgaataccgc tccactaatt aaaatgggta aaaaaggttt taatttcatc
    18541 gtaactcttc taatgcttca acgattttat ttggtgttgc ccactcatct ctatcaaagt
    18601 ccataataga tacttcgata tcaagtttat tttgaatctc taataataat ccaactgttt
    18661 ggaaagaatc aataatacct tcttcaaaaa tttctacgtc tggattttct tttacaatat
    18721 cattttctgc tacttctgct aataaattta atacttgttc tctaaattcc ataattaaat
    18781 tctcctttat tatataagtt tacctgagaa gattaaaaag ccaaatgtta caaagtggaa
    18841 tgtaatcaca atgctaagtg ctgttgtgaa accattttgc caacgtggcg gatgtttctt
    18901 acgccaacgt tcataatagc cataacctat aaacaatgct gcatggtata aaccataaac
    18961 aatgtaatac acttcgatac catgccaaat tcccattatg aagaagttga ttaagaatgc
    19021 cacgttagac attgcaaatt gactcttcaa taatttttta cgagacatgt agaataaaga
    19081 tctcatgtaa atacaatctc tgaaccagaa tgataatgtc atatgccatc tattccagaa
    19141 atctttaata tttttcgctt tgaaaggttt atcgaagttt ggtggtgttt taataccgaa
    19201 taaataacta aatgctatcg caaataaact ataacctgca aagtcaaaga ataaatataa
    19261 gctgtatgcg tacatatata accacaaatt gacaaagcca tgtaagtcta attgtaacgg
    19321 catgattgca tatgtgttaa taaagtaagc aacaatatat ttatacaaga aaccaagcat
    19381 aatcatgtga attgctttta atactaattc acgatattca ttgcctgttg gtactttttt
    19441 atcgtcttta acgaaacgtt tgtaacgatc gattggtcca gatgaaatcg ttgggaagaa
    19501 tgaaataaat tgaattaatt tccatacttt aatttctttg atagaaccat cacgaatttc
    19561 cataattaac tgcacacttt tgaatgtaac ataagaaatt cctaagaaac caacaaattc
    19621 aattaattta ctttcatgga agtgaatctg atgtccacct aaccatgtac tttgtaacac
    19681 tttcacaagt gctaatggca atattgataa aaccattaca gttacaaatt ttgaaaatga
    19741 attatttttt ggttttgaat gataataaaa cattatcaat aaaacttgcc atacgacgta
    19801 aataataaaa ctaattaatt gaacacttaa atacttttgg tcaaacagat tatgtttatc
    19861 agaagaaaag attaacacaa tcataaatgc tgtaactacg ccattataaa tgtaacttcg
    19921 cttacctaaa aatccaagta taatgactgg taataatgca attaaagcaa ttaagaagaa
    19981 tgtaaaatca ccatatggaa tcatccgtta attacctctg caattttctt tctgtcaatt
    20041 ttaccatttg aagtcaatgg caattgttcc atccattcaa actttctagg aatcatatac
    20101 tctggtaagc gtgatttcaa gtcattttta atatttttag tcatttctgc attgtccgta
    20161 acttcagtcg ttggcacaat tgcaccaatt aaatgaataa ctttatcatt tttatataca
    20221 ggtacaacaa tcgcttcttt tacgaactca gactggcgta attgtgtttc aatttcttct
    20281 aattccattc tgtagccatt caatttgatt tggaaatcaa tacgaccttg aatgaaccat
    20341 tgaccatttt caaacttcgc tttatcacca gtgtgatatg tacgaatacc gtcatcgaaa
    20401 ttaaatactt cagctgtttt ttggtcattt tttaagtatc ctaaacttac actttgacct
    20461 tcaataacaa gttcaccgtc atctgtagta gataatcttg cgcctagtct ttcaacgcca
    20521 acaggtaatg tcggatattg atctaagatt tcttgtgtaa tttgaatact tgtaactgct
    20581 accgtagctt cagttggacc atatgtgttg taaatcgtcg cacttgggaa acggctcact
    20641 aacgcttttg ctgctctgtg aggtagaatt tcaccacaga agaagaattc gttaagacta
    20701 ccatattgtt cttcattaag cgttggtaat aataaacaca tttccataaa tgatggtgtt
    20761 gatacccaaa tgttaatcgg tgttgctgtt agcatttcat ttaataattt aggtttatta
    20821 atcatgtttt tatctacaag atttaatgta ccgcctgatg ctaaacatgg ataaatagcc
    20881 attacagata aatcaaatga aaatggcgct tggttaagcc attgttgttt atttcctgat
    20941 ttattaagtt ctaacatcca ctcagtaaat tgaactaaac ttgcatattc aatttgaaca
    21001 cctttaggtt ccccagtaga accagatgta aagattgtgt atactgtgtc gttatcttta
    21061 atctgactat caaaaattac tgggtcttga gatgttttaa tatcttcaat tgtaaatact
    21121 tcgccttcta aactttcaaa tgattcatca gtcgtattaa atacaaactc tggttgaacc
    21181 ttgttaataa tcattttaat acggtcttca ggaattgaag tgtctacagg tacatatcca
    21241 catcctgctt taatggcacc aatcatccca acaatcatat atggtgacat gtgaccgaat
    21301 aaaatcatcg gtttcttact accttgtaat cgatgtgcta atttactaga ctcatccatt
    21361 aactgttgat aagttaattc atcagttgtg tgtctaacag caatgctttg tggatttgca
    21421 tccgcaaacg cttgcagctt gttaataata tctgtcatat taagtctccc tcattagaac
    21481 tcattataaa tgaagttatt gtgtgtgtcg ccactgccat aaattaaata taaagtaata
    21541 aatattgcca aatacaatag tgttaataaa tatggtttga atgcttcaac atatttatta
    21601 ggtggctgtt tacttttaga tttcatattg cacctcttaa agttcttagt aaaaacgcct
    21661 ttataaagac cgttcaatat aaaatacgtt ttaaaatttg ttttttacaa ttcattatat
    21721 cgatattcat aatgaaattc aattttaatt ttatagattc aacatagtaa ttggttgtca
    21781 tcactcaatt attgtaaatg atacactttg ttgagaacat cattcatttt aaaggttatt
    21841 taaacaataa acaattacag tctatataac aattttgtta tatacgtcaa aatcaaataa
    21901 actcatcaca ttaatatgac gagtttataa tgttattgaa ttatcatcag cgcaaatata
    21961 tacattcgca agtcaagcat aacatattta acaattgctt tgcttgtttt accaatgatt
    22021 aaaaaccata cttattttca atttactgga gtatgtggta cctgatttgt cataaccgct
    22081 tctatattat taatacataa ttgaatcata ttgtcccgtg ttgttactga tgcgctacca
    22141 atgtgtggtg taatcagaac attatcacgt cccattaatg gatgtgtatg atcaatcggt
    22201 tcatttgcta atacatctaa accacatgct aaaatttctt tattgtctag tgcatcgatt
    22261 aatgctgttt catctacaat ttgccctcta ccgatattaa taaaaattgc atcatttttc
    22321 atttgttcaa atgcttcagc attaaattta tgatgtgttt cttttgtaag tggcgctgta
    22381 cagatgataa aatcactttc tgctaacaac gtttcaaaag aaacatatgt tgcattaaag
    22441 tccgcctctg catctttatg tcttgatcga ttatgataaa gaatattagt attaaaccct
    22501 tgcaaccttc ttgcaaaagc tttaccaata tctcccatac catatattcc aatagttgaa
    22561 ttgaagacat ctttacctga caataaataa ggaccccagc tttgccatgc atctgcttct
    22621 acatattttt cagcttctac aatacggcgt gctatagcaa gcattaatgt aaatcctaat
    22681 tctgcagttg tttcagtaag tacatttggt gtattcgtga caaccacgtt attcgctgtt
    22741 gcactttcaa catcgatgtt gtcatatcct acagccatat tcgcaattac ttttaaattt
    22801 ggtgattgtg ctaaaatttc tgcatcgatc tgttcactta atgtaataaa acaagcatct
    22861 gcgtcacgaa gtgctgtcac aaattgatct ttaggcatag gcactaatga tttttgccac
    22921 ataacaacgt caccaagttt acttaattgt tgataaaatt tatctggaat tttcctcgaa
    22981 acaactattt ttaccaaatc aattcaccta ctttataaaa tgactatatc tatttttcta
    23041 attcagctat tgcttcattt aaatctttaa aagaatacgt tggtggcaca tttttatttt
    23101 gcacatcttc taacgtagat acacctgttt gtacatgaat cgtatccata cctacgttaa
    23161 taccagacat aatatcggta tcgtacaaat cgcctaccat agcaacttcg gatttatcta
    23221 atcctaaaat ttctaatgct ttaaccataa taatcggttc tggtttacca ataaattgtg
    23281 gcgatacacc tgtcgataca cttacaacac ttgtaatagc accattacca ggtaataaac
    23341 cacgctcttt aggaattgat acatcaggat ttgtagaaat aaatgttgca ccatttctta
    23401 cacctaacgt tgcaatcgca agcttttcat atgtaacttg ttcgtcaagt ccaataacta
    23461 cataatcaac atgctcgtca tttttaatga caagtcccgc ttcggttaac gcagtattta
    23521 aaccactccc acctaacata tatactgatg ctcctggtga ttgttctgaa atataatcag
    23581 cagtggctaa cgctgacgtt acaacctctt ctggtttagc atcaatgtgc atttcacgta
    23641 atttttcagt tacttgctca ggtgtttttg ttgaattatt agttacgtat aaatgaggaa
    23701 tgccttttac atttaaataa tcgatgaatt gttttgctcc atcaatctca tctgttccca
    23761 tatacattgt gccatctaaa tcgattaaat acgctttata ctgtttcatt attgattttc
    23821 tcctttacca aaagctgtta ctggtacatt ttcttgatgt aaaaatatcg ttacttctgt
    23881 aataaattgt ttgtaataag gtaatgcgtc atcaaacatt ggcatgataa ccttaatatc
    23941 taaggctgtg taatcatatg taaattgttt acgaatatcg acagttttat taatctcctg
    24001 ctgtgtttct ttagtaataa cattttctag ttctaatata tcaatcacat ctttataatt
    24061 accaggatcc cttaaaataa atgcatcgat aatcatattc cctatatcta ccgatgattc
    24121 tatcaacatt tgagcaatgc gttcaaatgc ataatgattg tgcttgctct catgataatc
    24181 atcagttaat gcttgtaaat aggctaattt ctgagttagt ttatctttgt ctacaaaata
    24241 cattccttgt cacctctcgc ttatatatta tcataatttt gcagtatact tcatttgtcg
    24301 ttatactaac tttactactt ttaaaaggag tcgctacaaa atgattgata tgtatttata
    24361 tgatgacaac gaagagagtc aagttcagtt tgttggtttt gttggcgagc atagtcgata
    24421 tgatttgatg ttagtccata caaatagaca ttatggtaag acactcgtac ttaatatgca
    24481 aacaaataaa ttcggtatta tcggtactga tgatttaaaa gaagaaggct atatcgctca
    24541 tattttaggt gtaaatgctg aagaaggcga tgaaattact gagtatttaa atgaagtcat
    24601 tcattaaggt tctccaccaa atgtggtggg tatataattt aaagaactat tttaaattac
    24661 aacttttaga gtttttatta ttaggcggcc agtccattat tgggcttggt tgtcttcttt
    24721 ttttctcctt tgtataagct gaaaatcatc attatacgtg ctttaaagtt gttgaaattt
    24781 ctgtaaccaa aagaaattcg cttgattaat tttatcttat tattaattcc ttctatagca
    24841 ccattattaa atgctgggta ataaattgta tttcttaaca tcctttgatg ttttctataa
    24901 tatttaacca ctttccatac acctttactc acagactttt tactaactga atttaaacga
    24961 ttaataaatt taggccaatt acataacctt agatcttttc ttaatccttg gacaagttcg
    25021 taggagcgtc gtagtatatc gtcttttgaa agcatgaatt ctacaatgtc agatgagcgt
    25081 ttataagcct taaaagattt attccatctg tatttactaa atatggtttt actagtatcc
    25141 atcaatagaa ctttccagtt attcttaaaa attgtataat taggtccttt tttattacgg
    25201 tattcattca taacttgtac acgatactta ttaagttccc tatttaaatg ttgaacgata
    25261 tggaatctgt caaaaataat agctgcatta ggaaataggt cgcgaaataa tcggacatag
    25321 ggttcataca tgtcaatagt aaccgtctta acttgttgtc gattttttaa atcgaatcgc
    25381 tcgaaatagg cacgcaagaa tcttgtagtt ctattttcta aaatatctat aacatcatga
    25441 gtatcattat ctataaaaat gaaactcatt gatccagtta catttttaac gcttttaaat
    25501 tcatccatag cgatgtgttc tggcaaacaa ttaaacggtt taattcgtaa tgaattagcc
    25561 gctttagttc taatacgact tacagtagat ggagagatac agttatcttc tgcagtatca
    25621 atctctgttt taactttagt agcttcttct ataacttttt gagtaacaca tcttgagata
    25681 aaacacgact catcaacaat tgacgtttta gcagtaaaag tgccattgca ttctaagcat
    25741 ttaaaacgtt gtttagctaa gttgaggtat gtgttatacc cttgagtttt aagtaaagta
    25801 atacgagaaa cacgcttacc atgtttatga atttgtcctt catttttaac tccacaacaa
    25861 taacaggccg aaggcttata cgttagtaca gcattaacca ctgttgcttt ttttcctttg
    25921 ataatgacgt caatttcttg gtttatatat tttatatttt catcttttat tcttagtaat
    25981 tctaaggtat cattacacat aggcgcactt gtctccttta gtttgggttt ggtcacttta
    26041 aattatagag gcaattgcgc tttttgtgta
//
LOCUS       DAOBBH010000017        25595 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.017, whole genome shotgun sequence.
ACCESSION   DAOBBH010000017 DAOBBH010000000
VERSION     DAOBBH010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25595
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.017"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(306..872)
                     /locus_tag="P6L01_000498"
     CDS             complement(306..872)
                     /locus_tag="P6L01_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDP3029592.1"
                     /translation="MKGLIIIGSAQVNSHTSALARYLTEHFKTHDIEAEIFDLAEKPL
                     NQLDFSGTTPSIDEIKQNMKDLKEKAMAADFLILGTPNYHGSYSGILKNALDHLNMDY
                     FKMKPVGLIGNSGGIVSSEPLSHLRVIVRSLLGIAVPTQIATHDSDFAKNEDGSYYLN
                     DSEFQLRARLFVDQIVSFVNNSPYEHLK"
     gene            complement(894..1763)
                     /locus_tag="P6L01_000499"
     CDS             complement(894..1763)
                     /locus_tag="P6L01_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDP3029593.1"
                     /translation="MIKLIATDMDGTLLNAAHEISQPNIDAIKYAQEQGITVVIATGR
                     AFYEAQAPVADTDLTVPYICLNGAEVRDETFNVMSTSHLNKSLVHKITNVLKDAGIYY
                     QVYTSRAIYTEDPQRDLDIYIDIAERAGQHANVERIKNGIQRRIDNGTLKVVDNYDAI
                     ENIPGELIMKILAFDGNLEKIDKASKILAESPNLAISSSSRGNIEITHSDAQKGIALE
                     TIAERLGIEMKDVMAIGDNLNDLSMLEKVGYPVAMENGAEEVKKIAKYVTDTNENSGV
                     GKAIMKLLREQQV"
     gene            complement(1910..2380)
                     /locus_tag="P6L01_000500"
     CDS             complement(1910..2380)
                     /locus_tag="P6L01_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HDP3029594.1"
                     /translation="MTNDIYFMTLAIEEAKKAAQLGEVPIGAIITKDDEVIARAHNLR
                     ETLQQPTAHAEHIAIERAAKVLGSWRLEGCTLYVTLEPCVMCAGIIVMSRIPRVVYGA
                     DDPKGGCSGSLMNLLQQSNFNHRAIVDKGVLKEACSTLLTTFFKNLRANKKSTN"
     gene            2447..3064
                     /locus_tag="P6L01_000501"
     CDS             2447..3064
                     /locus_tag="P6L01_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDP3029595.1"
                     /translation="MNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSD
                     FYEDISKWSFQTEMFFLCNRYKQFQDVTQLNQGVVSDYHIHKNKIFAKNTLSPVEFQK
                     FSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRSFEHQIEDEYLLKLKKDYREY
                     YESLQSNGSNVVLIDTTSIDFLKNEQDYEDILHIILPMIGDITNE"
     gene            3057..3719
                     /locus_tag="P6L01_000502"
     CDS             3057..3719
                     /locus_tag="P6L01_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDP3029596.1"
                     /translation="MNNYGIPQNAIITIAGTVGVGKSTLTQALADKLNFKTSFENVEH
                     NPYLDKFYSDFERWSFHLQIYFLAERFKEQKRMFEYGGGFVQDRSIYEDVDIFAKMHE
                     EEGTMSKEDFKTYSDLFNAMVMTPYFPKPDVMIYLECNYDEVIDRIIERGREMEINTD
                     PEYWKKLFKRYDDWINSFNACPVVRININEYDIHKDPESLNPMIDKIARIIQTYRQVD
                     TR"
     gene            complement(3835..4518)
                     /locus_tag="P6L01_000503"
     CDS             complement(3835..4518)
                     /locus_tag="P6L01_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDP3029597.1"
                     /translation="MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALR
                     QLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVE
                     GVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNAISLFDLIISTEADAYEKPNPKV
                     LSPLFEQYNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNS
                     AADILEALN"
     gene            complement(4768..5844)
                     /locus_tag="P6L01_000504"
     CDS             complement(4768..5844)
                     /locus_tag="P6L01_000504"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDP3029598.1"
                     /translation="MSQAVKVERRETLKQKPNTSQLGFGKYFTDYMLSYDYDADKGWH
                     DLKIVPYGPIEISPAAQGVHYGQSVFEGLKAYKRDGEVALFRPEENFKRLNNSLARLE
                     MPQVDEAELLEGLKQLVDIERDWIPEGEGQSLYIRPFVFATEGALGVGASHQYKLLII
                     LSPSGAYYGGETLKPTKIYVEDEYVRAVRGGVGFAKVAGNYAASLLAQTNANKLGYDQ
                     VLWLDGVEQKYIEEVGSMNIFFVENGKVITPELNGSILPGITRKSIIELAKNLGYEVE
                     ERRVSIDELFESYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNETGEITQKLY
                     DVYTGIQNGTLEDKNGWRVVVPKY"
     gene            complement(6180..7145)
                     /locus_tag="P6L01_000505"
     CDS             complement(6180..7145)
                     /locus_tag="P6L01_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDP3029599.1"
                     /translation="MKKIMITGALGQIGTELVVKCREIYGTDNVLATDIREPEADSPV
                     QNGPFEILDVTDRDRMFELVRDFEADSLMHMAALLSATAEKNPILAWDLNMGGLMNAL
                     EAARTYNLHFFTPSSIGAFGDSTPKVNTPQVTIQQPTTMYGVNKVAGELLCQYYFKRF
                     GVDTRSVRFPGLISHVKEPGGGTTDYAVEIYFKAVREGHYTSFIDKGTYMDMMYMDDA
                     IEAIIKLMEADDAKLETRNGYNLSAMSFDPEMVKEAIQEYYPNFTLDYDVDPIRQGIA
                     NSWPDSIDTSCSRGEWGFDPKYDLASMTKLMLEAIEQKDTVKNNN"
     gene            complement(7359..8996)
                     /locus_tag="P6L01_000506"
     CDS             complement(7359..8996)
                     /locus_tag="P6L01_000506"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HDP3029600.1"
                     /translation="MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIESELN
                     GLKIPHTYALQNSNDYLEIMEEGISYIVRESKIDPVNIVGIGIDFTSSTIIFTDENLN
                     PVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPK
                     IMEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNVRSNCGLGFKAFWEEETGFHYDLFD
                     KIDPKLSKVIQDKVSAPVVNIGEVVGKLDDKMAQKLGLSKETMVSPFIIDAHASLLGI
                     GSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFE
                     YVAKQAPKSYVDEAANRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLT
                     GCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMM
                     DIYANVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAE
                     KVQRYETLFKAYKALHDIHGYKKANIMKDIQSLRVEG"
     gene            complement(9155..10033)
                     /gene="hchA"
                     /locus_tag="P6L01_000507"
     CDS             complement(9155..10033)
                     /gene="hchA"
                     /locus_tag="P6L01_000507"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HDP3029601.1"
                     /translation="MSQDVNELSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEH
                     KGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLS
                     GYPVKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGH
                     AAVVGISESEDVQQTLDWALDNDRFIVTLCHGPAALLSAGLNREKSPLEGYSVCVFPD
                     SLDEGANIEIGYLPGRLKWLVADLLTKQGLKVVNDDMTGRTLKDRKLLTGDSPLASNE
                     LGKLAVNEMLNAIQNK"
     gene            complement(10303..11490)
                     /locus_tag="P6L01_000508"
     CDS             complement(10303..11490)
                     /locus_tag="P6L01_000508"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDP3029602.1"
                     /translation="MVQSLHEFLEENINYLKENGLYNEIDTIEGANGPEIKINGKSYI
                     NLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTE
                     AAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDL
                     RAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVM
                     GKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLA
                     PGDTKAITEAVKKLMDSTELHDKLWDNAQYLKNGLSKLGYDTGESETPITPVIIGDEK
                     TTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIAAYEKVGKEM
                     KLI"
     gene            11661..12836
                     /locus_tag="P6L01_000509"
     CDS             11661..12836
                     /locus_tag="P6L01_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDP3029603.1"
                     /translation="MLDWFQLANNKENKTIQLRRYLHQYPELSFEEFQTHDYIVNQLS
                     QLSCDIETPIGRNGIKATFKGLGTGPTIALRADFDALPVEELNDVPYKSKNPGCMHAC
                     GHDGHTAILLTVAEILDEHKHLLEGNVVLIFQYGEEIMPGGSQEMIDAGCLENVDRIY
                     GTHLWSGYPTGTIHSRAGAIMASPDEFSVTIKGRGGHGAKPHETIDPIVIMAEFILSA
                     QKIISRTIDPVKQAVLSFGMIQAGTTDSVIPDQAFCKGTVRTFDSDIQNHVMDKMDKL
                     LQGLAIANDINYDLNYIKGYLPVHNNEKAYQVIKEATNDLHVRFNESDLMMIGEDFSH
                     YLKVRPGAFFLTGCGNESKGITAPHHNPKFDIDEKSLKYAVAVFLKIIELEQVFKTN"
     gene            complement(13118..14302)
                     /gene="tuf"
                     /locus_tag="P6L01_000510"
     CDS             complement(13118..14302)
                     /gene="tuf"
                     /locus_tag="P6L01_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDP3029604.1"
                     /translation="MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVA
                     QSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGG
                     ILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSE
                     YDFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDV
                     FSITGRGTVATGRVERGQIKVGEEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIG
                     ALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFR
                     TTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEI
                     IK"
     gene            complement(14519..16600)
                     /gene="fusA"
                     /locus_tag="P6L01_000511"
     CDS             complement(14519..16600)
                     /gene="fusA"
                     /locus_tag="P6L01_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDP3029605.1"
                     /translation="MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETH
                     EGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAA
                     PIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAV
                     AETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVID
                     YLPSPLDVKPIIGHRASNPEEEVIAKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGT
                     MTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGE
                     KNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVII
                     GGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGD
                     VHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKL
                     YDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVT
                     SRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSI
                     AEDIIKKNKGE"
     gene            complement(16723..17193)
                     /gene="rpsG"
                     /locus_tag="P6L01_000512"
     CDS             complement(16723..17193)
                     /gene="rpsG"
                     /locus_tag="P6L01_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDP3029606.1"
                     /translation="MPRKGSVPKRDVLPDPIHNSKLVTKLINKIMLDGKRGTAQRILY
                     SAFDLVEQRSGRDALEVFEEAINNIMPVLEVKARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNYARLRGEKTMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFAHYRW"
     gene            complement(17259..17672)
                     /gene="rpsL"
                     /locus_tag="P6L01_000513"
     CDS             complement(17259..17672)
                     /gene="rpsL"
                     /locus_tag="P6L01_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDP3029607.1"
                     /translation="MPTINQLVRKPRQSKIKKSDSPALNKGFNSKKKKFTDLNSPQKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNNIEINAYIPGIGHNLQEHSVVLVRGGRVKD
                     LPGVRYHIVRGALDTSGVDGRRQGRSLYGTKKPKN"
     gene            complement(17770..18024)
                     /locus_tag="P6L01_000514"
     CDS             complement(17770..18024)
                     /locus_tag="P6L01_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HDP3029608.1"
                     /translation="MSKEKVARFNKQHFVVGLKETLKALKKDQVTSLIIAEDVEVYLM
                     TRVLSQINQKNIPVSFFKSKHALGKHVGINVNATIVALIK"
     gene            complement(18161..21757)
                     /gene="rpoC"
                     /locus_tag="P6L01_000515"
     CDS             complement(18161..21757)
                     /gene="rpoC"
                     /locus_tag="P6L01_000515"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDP3029609.1"
                     /translation="MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFG
                     PTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIP
                     SRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAK
                     MGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMI
                     LDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEK
                     RMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSV
                     IAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVL
                     EEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHV
                     PLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFN
                     NTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSF
                     AYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFN
                     RFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQF
                     NRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLA
                     GMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRR
                     LVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIRHPETDEII
                     IRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAV
                     GTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGV
                     VEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYL
                     SVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSL
                     VDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAI
                     KGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE"
     gene            complement(21921..25472)
                     /gene="rpoB"
                     /locus_tag="P6L01_000516"
     CDS             complement(21921..25472)
                     /gene="rpoB"
                     /locus_tag="P6L01_000516"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDP3029610.1"
                     /translation="MAGQVVQYGRHRKRRNYARISEVLELPNLIEIQTKSYEWFLREG
                     LIEMFRDISPIEDFTGNLSLEFVDYRLGEPKYDLEESKNRDATYAAPLRVKVRLIIKE
                     TGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDA
                     TIIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLR
                     NTLEKDGTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTN
                     KKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMDVLESNANSEVFELH
                     GSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLS
                     GIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINI
                     RPVIASIKEFFGSSQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYS
                     HYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITDQIDYLTAD
                     EEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATAC
                     IPFLENDDSNRALMGANMQRQAVPLMNPEAPFVGTGMEHVAARDSGAAITAKHRGRVE
                     HVESNEILVRRLVEENGVEHEGELDRYPLAKFKRSNSGTCYNQRPIVAVGDVVEYNEI
                     LADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEA
                     RDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAE
                     ERLLHAIFGEKAREVRDTSLRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIV
                     QKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDIMLNPLGVPSRMNIGQVL
                     ELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRI
                     SVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMD
                     EQDNEIEMTDVDDDDVVERKVDLQQNDAPETQKEVTD"
BASE COUNT         7791 a         4917 c         3903 g         8984 t
ORIGIN      
        1 ttacaatgtt atttgctaca aatattaata tgaatcacag tttaagaatt gcaattttta
       61 atactttttt tctttaattg ctttaaatta tttatctata tttttaaaac tctcttttaa
      121 tctaagcttg ttttcaattc gctattacat cttattttaa ttaataacat tgctcacaac
      181 agtaatctca aaggcttact attggtgcat tagaacaaaa aaaggcatgt gacttaaaag
      241 cattctcaat gaaatacctt aagctacata cctattttta atgtatattt acatattttt
      301 taatattatt ttaaatgttc atatggacta ttattcacaa aagatacaat ttgatcgaca
      361 aataatcttg ctcgtaattg gaattcacta tcatttaagt aatatgaacc atcttcattt
      421 ttagcaaaat cagaatcatg tgttgctatt tgagttggta cagcaatgcc tagtaaactt
      481 ctgacgatta ctcttaaatg tgacaatggc tctgaactaa caataccacc actatttcct
      541 attaagccta caggtttcat tttaaaataa tccatattta gatgatctaa tgcatttttc
      601 aatattccag aatatgaacc atgatagttt ggcgttccta atattaaaaa gtccgccgcc
      661 attgctttct cttttaaatc tttcatattt tgtttgattt catcaataga cggtgttgtt
      721 cctgaaaaat ctaattgatt taacggtttt tctgctaaat cgaatatttc cgcttcaata
      781 tcatgtgttt taaaatgctc agttaagtat cttgctagtg cacttgtatg tgaattcact
      841 tgtgcactgc caataataat taatcctttc atatttgacc cctctttttt ttattaaact
      901 tgttgttcac gtaataattt cataatagct tttccaacac cactattttc attcgtatct
      961 gtgacatatt tcgctatttt tttaacttct tctgcaccat tttccatcgc aactggatag
     1021 ccaactttct ctaacattga taagtcattt aaattgtcac ctattgccat gacatctttc
     1081 atttcaatcc ctaatctttc ggcaattgtt tctagcgcaa tacctttttg tgcatctgaa
     1141 tgcgttattt ctatatttcc tctcgaagat gatgatatag ctaaattcgg agattcagct
     1201 aaaattttac tagctttgtc aattttttct aaatttccat caaatgctaa tattttcata
     1261 attaattcac caggtatgtt ttcaatagca tcataattat caacaacttt caacgtacca
     1321 ttatctatgc gtctttgaat accattttta atacgctcaa cgtttgcatg ttgacctgca
     1381 cgctcagcaa tatctatgta aatgtctaaa tctctttgtg gatcttcagt ataaatcgca
     1441 cgactcgtgt atacttgata ataaatacct gcatctttta aaacatttgt aattttgtgt
     1501 actaacgatt tattaaggtg tgaagtgctc attacattga aagtttcatc acgtacttca
     1561 gcaccattca aacaaatata tggtactgtt aaatctgtgt cagcaactgg tgcttgtgct
     1621 tcataaaatg ctcgacctgt cgcgataaca accgttatcc cttgttcttg agcgtattta
     1681 atcgcatcaa tattaggttg agaaatttca tgtgctgcat taagtagcgt gccatccata
     1741 tcagtggcta ttagttttat cattatgtta cctcgtttcg taaatttaaa atcttgttct
     1801 taaataagaa tatatactca gcgcacatac ttttctatta acatttatat tgtcattaat
     1861 ttatcatata atgtaattct aacaaatttt aattagtatg tactatcgtc taattggtgg
     1921 atttcttatt ggctcttaag tttttaaaaa atgttgttaa taatgtgcta catgcttctt
     1981 taagtacacc tttatcaaca attgcacgat gattaaaatt agattgttgc aataaattca
     2041 ttaaactgcc actacaacca cctttaggat catctgcgcc atagacgact cttggaatgc
     2101 gactcattac aattattcct gcgcacatga cacatggttc taaggttaca tataatgtgc
     2161 aaccttctaa acgccaacta cctaacactt tggctgcacg ttcaattgca atatgttcag
     2221 catgcgccgt tggttgttgt agtgtttctc ttaaattatg tgctctagcg ataacttcat
     2281 catctttagt gatgatagca cctataggta cttcgcctag ttgagctgct tttttagctt
     2341 cttcaatcgc taatgtcata aaatatatat catttgtcat ttatgtccag atacctcact
     2401 tatggtacaa tactcaatgt ctattttaac atttggagat gtgaatatga acaaaccttt
     2461 tatagcaatt gaaggtccta ttggcgtagg taaatcttca cttgcacaca aattaagtca
     2521 aactttagat ttttatgaag aaaaagaaat catcacagaa aatccatttt tatcagactt
     2581 ttatgaagat atctctaaat ggagttttca aactgaaatg ttctttttat gcaatagata
     2641 taagcaattt caagatgtaa cacaactaaa tcaaggtgta gttagtgatt atcatataca
     2701 taaaaataag atatttgcta aaaatacttt gagtcctgtt gaatttcaga aattcagtaa
     2761 aatttatgat attttaactg aagatatgat tatgccgaat atgattatct ttttagatgc
     2821 agaccttgat gtgttaaaat ctagaattgc taaacgtaac cgtagttttg agcatcaaat
     2881 agaagatgaa tatctgttaa agttaaaaaa agattatcgt gagtattatg agtccttaca
     2941 aagtaatggt tcaaatgtag ttttaatcga tacaacttct attgattttc ttaaaaatga
     3001 acaagattac gaagatatat tacatattat attacctatg ataggagata ttaccaatga
     3061 ataattacgg tattccacaa aatgccatta taaccattgc aggtacagtt ggtgttggaa
     3121 aatcaacact aacgcaagca cttgcagata aattaaactt taaaacgtct tttgaaaatg
     3181 tcgaacataa tccatattta gataaatttt atagcgattt tgaacgatgg agtttccatt
     3241 tgcaaattta cttcttagct gaacgtttta aagaacaaaa gcgtatgttt gaatatggtg
     3301 gtggctttgt ccaagatcga tcaatttatg aagatgttga tatttttgca aaaatgcatg
     3361 aagaagaagg cacaatgagt aaagaagatt tcaaaacata ttcagactta tttaatgcca
     3421 tggtcatgac accttatttt cctaaaccag atgtaatgat ttatttagaa tgtaactatg
     3481 atgaggtcat tgatcgtatt attgaacgtg gtcgcgaaat ggaaattaat acagaccctg
     3541 aatactggaa aaagctattt aaacgctatg acgattggat taatagcttt aatgcatgtc
     3601 cagttgtacg tatcaatatt aatgaatatg atatccataa ggaccccgaa tctttaaatc
     3661 ctatgataga caaaattgct cgaattattc aaacatatcg acaagtagat acacgataaa
     3721 agactaaaga catagcgtat atgtttatat tcaatgtata ttccatagat atcatcgatt
     3781 acattatcaa ttctatcgaa tacattaatt cacatataca ctatgtcttt ctttttaatt
     3841 taaagcttct aaaatatctg ccgcactatt taaaataata tcagcttcat gtaattcttc
     3901 ttttgttgca atacctgtta atacacctat tgccatacct aaatttgcat tacttgctgt
     3961 cttcatatca ttagcagtgt ctcctactat agctactttc tgaggatcta cattatattg
     4021 ctcaaataaa ggcgataata ctttaggatt tggcttctca taggcatccg cttcggtaga
     4081 aatgatcaaa tcgaacaacg agatagcatt ggtatgtgct aaaaattgtt ctacaccttt
     4141 tttagtatca ctcgtaacaa taccaagttg atagcctttt gctttcaaat cgataagtgc
     4201 ttctttaaca ccttctaccc aattaatttc aggaatacgt tcatctacca gcttttgact
     4261 tgttgacttg gaccagtcgg ttgtatcttg tcccgtcaca tcattaaatg cctggataat
     4321 ttgttgtaaa gatcctgaac ccatcactga ttttggatca atagattctt taatgacacc
     4381 gagttgtctt aaagcagctt ctttattatg tactgggaaa gtctcaagca atgattgtac
     4441 aaatcgtacc cctatttttt cccaacttct atcaaattca attaacgtac catctttatc
     4501 aaataatatc cattccattg atatcaatac tcctatttat ttatttcgta ttatgctgat
     4561 tctatgatat tcgttatccc ctgaaaatga actcgtagta ttgttctatt taaatattga
     4621 attaaatata ataataagtg aaatcccctt caatacttaa caataaacat tgtaaactta
     4681 atttattacc atgcttcgct tcattgaaag ggattttagt catgattaac ttttacatat
     4741 tgttttcatg attatattca atttttatta atattttggt acaacgactc tccaaccatt
     4801 tttatcttct aaagtaccat tttgaatacc agtatagacg tcgtataatt tttgagtaat
     4861 ttcaccagtc tcattattat taataacgat ttcacgatct tcgtatctca atgtacccac
     4921 aggtgaaata actgctgcag taccactacc aaatacttct gttaactcac ctttatcata
     4981 tgattcgaat aattcatcga ttgaaacgcg gcgctcttcg acttcatatc ctaagttttt
     5041 agctaattcg ataatagatt tacgtgtaat accaggtaaa atactgccat tcaactctgg
     5101 tgtaattact tttccatttt caacgaagaa aatattcatg ctaccaactt cttcgatata
     5161 tttctgttca acaccatcaa gccataatac ttggtcataa cctaatttat ttgcatttgt
     5221 ttgtgctaat aaacttgccg catagttacc tgcaactttt gcaaagccta caccgccacg
     5281 aacagcacgc acatattcat cttctacata gattttagtt ggttttaaag tctcaccacc
     5341 ataatatgca cctgaaggag ataaaataat taataattta tactgatgtg atgcaccaac
     5401 gccaagtgcc ccttctgttg caaaaacaaa tggacgaata tataatgatt gaccttcccc
     5461 ttcaggaatc caatctcttt ctatatcaac taattgtttt agcccctcta acaattctgc
     5521 ttcgtctact tgaggcattt ctaatcgtgc taacgagtta ttaagacgct taaaattttc
     5581 ttcaggacgg aaaagtgcaa cttccccatc tcttttatat gcttttaatc cttcgaatac
     5641 cgattgacca taatgaacac cttgtgcagc aggtgaaatt tcaataggac cataaggtac
     5701 tatcttcaaa tcatgccatc ctttatctgc atcataatca taactcaaca tataatcagt
     5761 aaaatattta ccaaaaccta gttgagatgt atttggtttt tgttttaatg tttctcgtcg
     5821 ttcaacttta actgcttgtg acatggtgat tgcctcctaa taatattgta taagaatttg
     5881 tttaacttaa attataacaa tccatatttt gctgttcaat aaattttcta aaaattcaaa
     5941 attaattaac agattttcta gaaagactat atcttttagt ataaacgtat taatttcaca
     6001 gagacaagta atctgtgttt tactaatata ctttacatac aaaaaactct ttactttaaa
     6061 atgaactaag ctcgcgaatt caatatgtat aatgaataat attagaattc atgcactagt
     6121 ttattaaaat aaagagtaat ttaaaatatc attccgtgta ttaaagtgaa tggaaatgat
     6181 tagttattat ttttaacagt atctttttgt tcaatagctt ctaacattaa tttagtcatg
     6241 ctcgctaaat catatttagg atcaaatccc cattcgccac gtgaacagct tgtatcaata
     6301 gaatccggcc aactattagc gataccttgt ctaataggat caacatcata atctaatgta
     6361 aaatttggat agtattcttg aattgcttct tttaccatct ctggatcaaa actcattgcg
     6421 ctcaaattat aaccgtttct agtttctaat ttagcgtcgt ctgcttccat aagtttaata
     6481 attgcttcaa ttgcatcatc catatacatc atatccatat acgtgccttt atctatgaag
     6541 cttgtataat gaccctctct tactgctttg aagtatattt caacagcata gtctgtagta
     6601 ccgccacctg gctctttaac atgcgagatt aaacctggga atctaacact tcttgtatct
     6661 acaccaaaac gtttgaaata gtattgacac aataattctc cagctacttt atttacacca
     6721 tacattgtcg taggttgctg aatcgttact tgtggcgtat taactttagg agttgagtct
     6781 ccaaatgcac caattgaact tggtgtgaaa aagtgcaaat tataagttct tgcagcttct
     6841 aatgcattca ttaatccacc catatttaaa tcccaagcta gaattggatt tttctctgca
     6901 gttgctgata ataatgctgc catatgcatt agactatccg cttcaaagtc cctaactaac
     6961 tcaaacatgc ggtcacgatc tgttacgtct aaaatttcaa atggtccatt ttgtacaggt
     7021 gagtctgctt caggttccct aatatctgta gcaagaacat tatctgtccc ataaatttct
     7081 ctgcacttaa caactaattc tgtaccaatt tgtcctaatg caccagtaat cataattttt
     7141 ttcatagaaa tatctccttt gtctcttcta tatagctata gtccgtcaca agcggacata
     7201 atattcattt tcataataat tataatataa aagcgctttc ttgtatatat gacatgtaca
     7261 tgttgctgat gtttctgtaa atggaaattc tagttgtatt aattgatttt agtaatttat
     7321 agcgtttatt attgctaatt actgtcaaat taaatttttt atccctcaac tcttaaactc
     7381 tggatatctt tcattatatt agctttttta taaccatgga tatcatgtaa agccttataa
     7441 gctttaaata atgtttcata cctttgtact ttttccgctt ctggattaat ttcgtataaa
     7501 attggctctt tcatagcatc aactgcatca ttaattgaat tatgtgcgcc accactgact
     7561 gcacctaata ttgctgcgcc tattgctggt gcatactcac tatccataac aattagtttt
     7621 ttattcagta cattcgcata gatatccatc ataacagcat tctttttagg tataccgcca
     7681 catgcaaata ccttttctac ttccatatgc caatcttgat actgttgcat aatcatctta
     7741 gtaccaaatg ctgtagcttc taaatatgct ctataaatat cctcatgctt agtttgtaaa
     7801 gttaatccaa agatacaacc tgttaaattg ctatcactta atacacttcg atttccatta
     7861 tgccaatcaa gagcaatgag cccactttca cctggcattt gatgttttat cttttcattc
     7921 attaattcaa atacagtcat atttctattt gctgcttcat ctacatatga ctttggtgct
     7981 tgcttagcga catactcaaa caaatcacct actgctgatt gccccgcttc ataagcaaat
     8041 aattctggaa taattgctcc ttttacagaa cctgatatac ctggcacttg atgttgcttt
     8101 tcatttaaca taagatggca tgtgcttgtt cccatcacca tagtcatttc tttatctttt
     8161 tcagacccaa tacctaataa actagcatgg gcatcaataa taaaaggact taccatagtc
     8221 tcttttgata atcctaattt ctgtgccatt ttatcgtcca gtttccctac tacttcacca
     8281 atattaacaa ccggtgcaga tactttatct tgaattactt ttgataattt ggggtctatt
     8341 ttatcaaata aatcataatg aaaccctgtt tcttcttccc aaaatgcttt gaaacctaat
     8401 ccacaattcg agcgtacatt tttattagtt aatttattta caatccaatc gcccgcttcc
     8461 ataatataag ccgttttttc cataatttct ggtgctcgat tcatgacttc cattattttg
     8521 ggaatcatcc attcactact aacattatat ccataatggc ctaaccactt attattattt
     8581 tcaatagcag tttgatataa tttctctgct tctttatatg caccatgatg tttccaaagt
     8641 ttcacatacg catgtggatt gtttttaaat tgttttaaat tatgtaccgg gttaaggttt
     8701 tcgtcagtaa aaataatagt agatgaagta aagtctatac caatacctac tatattgact
     8761 ggatctattt ttgattcacg tactatatat gatattcctt cttccataat ttctaaataa
     8821 tcattactat tttgaagtgc atatgtatgt ggtattttca aaccatttaa ttcactttca
     8881 atgacaccat gtgtatatgg tttcacaaat tttgatacta cttgaccgtt agttgtatta
     8941 attaaaaaca cacggcctga agcagttcca taatcaattc caatgctata agacatcatg
     9001 tttcctccat atcgctagtt gcggttatta gttttgcaag cgttttctta aatcatactc
     9061 ataataggtt ctattttaca agtatcactt aaattttgcc aaaaaaaaga gtcctcatac
     9121 atttacatat gaggctcctc taattaatat ttaattattt attttgtatt gcatttaaca
     9181 tttcattaac tgctaatttt cctaactcat ttgaagctaa aggactgtca cctgttaata
     9241 atttacgatc ttttaacgtt cttcctgtca tatcgtcgtt aactactttt aatccttgtt
     9301 tagttaataa atcagcaact aaccatttca agcgtccagg taaataacct atttcaatat
     9361 ttgcaccttc atctaatgag tcagggaaga cacaaacaga gtatccttct aatggagatt
     9421 tttctctgtt aagccctgct gaaagtagtg ctgctggtcc atgacataat gtaactataa
     9481 agcggtcatt gtctaatgcc caatctaatg tttgttgaac gtcctcactt tcagaaatac
     9541 caacaactgc agcatgtccg cctgggataa agacagataa atagtctgaa tcaggtccta
     9601 attcattttt aatcacatct gctaattttt ttggctgttt taatttttct ttcaatttat
     9661 tataagtact tataactgcc tcgtcttcag ttggcatagc ccataattct aatttaactg
     9721 gataaccaga taatgtcgca acatcaacgt caaaacctgc ttccattaaa tgatgtaaag
     9781 gtaataacat ttcaacagga tgattacccg tagagaacat ttttccattt tccaataata
     9841 catatctctc ttcagcagca atcatcaata ctttccattt accatcttta taggcacctt
     9901 tgtgttcaac accatcaaaa tctgttttag gtgctgtata ttgactaagg gaatatggtg
     9961 atgggaaaaa tgcgttatct tctgctttat ctggcgttgg ttgcttactt aattcattta
    10021 catcttgtga catagtttat tcctactttc tctatatagt tactactagt atagacatat
    10081 actacacact ataaacatgt attactatta aaatactcat tatcaaaaat aatcaaaatg
    10141 aatttctttg taaaaaatac aactaaattc tacaagatgc ttttaaaatt caaacttcaa
    10201 ttttggggag cactaaacat tgttataaga acttactgtt aataaactaa acattaaaaa
    10261 aagcccacga caatatttgc cgtgggaata aataaataaa tattaaatca acttcatttc
    10321 ttttcctact ttttcataag ccgcaattgc ttcatctaac atgtcttttg tatgtgcagc
    10381 tgtaggcata tttcttacac gtcctgtacc tcttggtact gttgggaaaa cgatagattt
    10441 cacatagaca ccttcgtctt ttaaacgctt actaaattct tgagttgttt tttcatcacc
    10501 aataattact ggtgtaattg gagtttctga ctcacctgta tcatatccta attttgacaa
    10561 tccatttttt aaatattgtg cattgtccca taatttatca tgtaattcag ttgaatccat
    10621 taacttttta actgcttcag ttattgcttt ggtatcccca ggtgctaatg atgtagagaa
    10681 taagaatggt cgtgattgtg cttttaacca atctattaac tcttttgtac ctgctacata
    10741 gccgccaacg acaccaattg ctttagaaag cgtacctatt tggaaatcga ttttatcttg
    10801 taaaccaaaa tgtttaaccg taccagcgcc tttacccata acacctgaac catgagcgtc
    10861 gtcaacataa gttaataaac caaattcttc tgcaatttct acaatttcag gtaatttagc
    10921 cacatcacca tccatactaa aaacgccatc agtgatatac atcactttat tgtattgacc
    10981 tgattcaact gcttcttttg ctttcgcacg taaatcatcc atgtctgaat ggttaactcg
    11041 aataatttta gctttagata agcgacatcc atcaataatt gatgcatgat taagctcatc
    11101 tgataaaata gcatcatttt tattcatgac agctgaaata gcagccatat tacaattaaa
    11161 tcctgactga taagctattg cagcttctgt tcctttaaat tttgctagtg tttcttctaa
    11221 ttcgtcgtgt aaatctaatg taccattgat tgtacgaaca gcgcctgcac ctacaccatg
    11281 tgtatcaata gctgcttttg cagctgattt caaatcttca tttgttgcta gtcctaaata
    11341 attatttgaa gataagttaa tgtatgattt cccattgatt ttgatttctg gtccgtttgc
    11401 accttcaatt gtatctattt cattatacaa accattttct tttagataat ttatattttc
    11461 ctctaaaaac tcatgtaatg attgaaccac agtatataat cccccttaag tattgtttgg
    11521 taataccttt atcatacagt aattctacat aacttgaaag ccttatcatt catagataaa
    11581 atatctcaaa gcaatcattt agtacgataa tttcataaaa tgttatacta taaacattat
    11641 tttagaaagt agtgataagg gtgttagatt ggttccaact agcaaataat aaagaaaata
    11701 aaacaattca actcagacgt tatttacacc aatatcctga gttatctttt gaagaatttc
    11761 aaacacatga ttatattgtt aaccaattaa gccagttatc ttgtgatatt gaaactccta
    11821 tcggccgcaa tggtatcaaa gccacattta aaggcttggg cacaggacct accattgcat
    11881 taagagctga ctttgatgcg ttacctgtcg aggaattaaa cgatgtgcct tataagtcta
    11941 aaaatccagg atgtatgcat gcgtgtgggc atgatggtca tacagcaatt ttacttactg
    12001 tagcagagat tttagatgaa cataaacatt tattagaagg taatgttgta ttaatattcc
    12061 agtacggtga agaaatcatg ccaggtggtt ctcaagaaat gatagatgct ggatgtctgg
    12121 aaaatgtaga tagaatttac ggtacacatt tatggagtgg ttatcctact ggaactattc
    12181 attcacgtgc gggggcaatc atggcctctc cagatgaatt tagcgtcaca attaaaggtc
    12241 gtggtggtca tggtgccaaa cctcatgaaa ccattgaccc aatagtaatc atggcagagt
    12301 tcattttaag tgcacaaaaa ataatttcac gtacgatcga tccagttaaa caagctgtac
    12361 tttcatttgg aatgattcaa gcaggtacca cagatagcgt tattcctgat caagcatttt
    12421 gtaaaggtac agttagaact ttcgattcag atatacagaa tcatgtaatg gacaaaatgg
    12481 ataagcttct acaaggttta gcaatagcaa atgatattaa ctatgatttg aattatatta
    12541 aagggtattt gcctgtccat aataacgaga aagcatatca agttattaaa gaagcaacaa
    12601 atgacttaca tgtacgcttt aatgaatcag atttaatgat gattggagaa gatttttcac
    12661 attatttgaa agtacgacca ggtgcattct ttctgactgg ttgtggtaat gaaagtaaag
    12721 gtattacagc gccacaccat aatcccaaat ttgatattga tgaaaaatca ttaaaatacg
    12781 ctgtagctgt atttttaaaa atcattgaat tagagcaagt atttaaaaca aattaagcat
    12841 ttaaattaat tgaaagatat gaatctggga cataaactaa tgaaatgctc ccttcaaagt
    12901 agacattgaa aaaatgaaaa ctttgaaggg agcattttac aatgtccaag actcaacaat
    12961 tttatttaaa cagtattatc agttataagt tttattagtc agtctttcat caattattgt
    13021 tcttctaaat cgaatagcca ttacaaaatt tacacattaa aaaaagaccc ctcgattgag
    13081 ggatcttctt tttatataaa atctaagaaa ttagaaatta tttaatgatt tcagtaacaa
    13141 cgcctgatcc tacagtacgt ccaccttcgc ggattgagaa acgagtaccg tcttcaatcg
    13201 cgattggagc gattaattct actgtcattt caacgttatc accaggcatt accatttcag
    13261 taccttctgg taagtgaaca acaccagtta cgtcagtagt acggaaatag aattgtggac
    13321 gatagtttga gaagaatgga gtgtgacgtc caccttcgtc ttttgataat acgtatactt
    13381 ctgctttaaa ttcagtatgt ggtgtaattg aaccaggagc agctaatact tgaccacgtt
    13441 gtacgtcttc acgagcaaca ccacgtaata atgcaccaat gttgtcacca gcttcagcgt
    13501 agtctaataa tttacggaac atttcaacac ctgtaacagt tgttttagat gtgtcatgta
    13561 aaccgatgat ttcaacttct tcaccaactt tgatttgacc acgttcaaca cggcctgtag
    13621 caacagtacc acgaccagtg attgagaata cgtcctcaac tggcatcatg aatggtttgt
    13681 cagaatcacg ttctggagtt ggaatgtaag tatctacagc ttccattaat tctaagattt
    13741 tttcttcgta ttgagcatcg ccttctaaag cttttaatgc tgaaccagcg attacaggta
    13801 catcgtcacc tgggaagtca tattcgctta ataagtcacg aacttccatt tctactaatt
    13861 ctaataattc ttcatcgtca accatgtcaa ctttgtttaa gaatactact aatgctggta
    13921 caccaacgtt acgtgataaa agaatgtgtt cacgagtttg tggcattgga ccgtcagcag
    13981 cagatactac taagataccg ccgtccattt gagcagcacc agtgatcatg tttttaacgt
    14041 agtcagcgtg tcctgggcag tcaacgtgag cgtagtgacg tttgtcagtt tggtactcaa
    14101 tgtgagaagt attgattgtg ataccacgtt ctttttcttc tggagcgttg tcaatcatgt
    14161 catatgattg tgcaactgag tcaccatttt ttgctaatac agtagcgatt gctgctgtta
    14221 atgttgtttt accatggtca acgtgaccga tagtaccgat attggcatgt tctttagaac
    14281 gatcgaattt ttcttttgcc attataaaat ctctcctatt ctaattaaaa ataatattta
    14341 aatttatctc tcatgatagt ttctcaccat catgagaaat tatttatagc gatgcaaaaa
    14401 gaattttaca attcttttat aatgcattca ccccaaaaaa tcaaactata aagatgatag
    14461 cttacattta agctcacctt gtattttaac ctaggctagc tagtcaaaac aagttatatt
    14521 attcaccttt atttttcttg ataatatctt cagcgattga ttttggaact tcagcatagt
    14581 gatcgaagta catagtgtaa gtaccgcgac cttgagtgtt tgaacgtaat gatgttgcat
    14641 aaccgaacat ttctgaaagt ggtacataag cattaacaac ttgtgcatta ccacgaggtt
    14701 ccataccatc aacacgtcca cgacgagatg ttacgtcacc catgatatca cccatgtact
    14761 cttcaggcat ttcaatagtt actttcatca ttggttctaa gattacagga tcacattttt
    14821 tagcagcttc tttaagtgct aatgatgcag caattttgaa ggccatttca gatgaatcga
    14881 catcatggta tgaaccatca tataatttag ctttaacatc aattaaagga taacctgcta
    14941 agacaccatt ttccatagca tctttaagac cagcttctac tgatggaatg tattcacgag
    15001 gaactacacc accaacgata gcgttttcga attcgaaacc tgcgcctgtt tcgtttggtg
    15061 tgaattcaat gtgaacatca ccgtattgac cacgaccacc agattgacga gagaatttac
    15121 cttgaacttg tgcagatgat ttgaatgttt cacgatatga aaccattgga gcacctacgt
    15181 tacattcaac gttgaattct ttcttcatac ggtctactaa gatgtctaag tgaagctcac
    15241 ccataccacc gatgataact tgtccagttt cttcgtcagt gtgtgcatgg aatgttgggt
    15301 cttcttcttg taatttaact aaagcttgag tcattttatc ttggtcagct ttagattttg
    15361 gctctactga taagtgaata actggctctg ggaattccat tgattccaag ataatgtcat
    15421 ttttctcacc acataaagta tcaccagtac ctgtatcttt aagacctacc gcagccgcga
    15481 tatctccaga gtatacagta tcgatttctt gacgtgagtt agcgtgcatt tgtaataaac
    15541 gacctacacg ttcacgttta cctttagtag agttcttaac gtatgaacca gatgtcattg
    15601 tacctgaata cacacggaag aatgttaatt taccaacata agggtcagtc ataactttga
    15661 acgctaatgc agcgaattca gctgaatcgt ctgctttcgc gattacttct tcttcagggt
    15721 tgctagcacg gtgaccaata attggtttaa cgtctagtgg tgaaggtaag taatcaatta
    15781 cagcgtcaag cattaattga acacctttgt ttttgaaagc tgtaccacaa agtactgggt
    15841 agaattctac gttagtagta gcttggcgga tagcttcttt taattcagaa actgaaattt
    15901 cttcgtcacc aagatatttt tccattaatt cgtcgctagt ttctgcaact gcttcgatta
    15961 agctagcacg agcttcttca gctctatcta agtggtcttc aggaatttca atttcttcaa
    16021 tttcagtacc taaatcattt gtatatttga aacatttcat ttcaactaag tcaatgattg
    16081 cttcgaattc gtcttccgca ccaattggta attggattgg agcagcgtta gcttgtaaac
    16141 gatcatgtaa tgtacttaca gagtattcga agttagcacc taatttgtcc attttgttta
    16201 caaatacgat acgtggaaca ccataagttg tagcctgacg ccaaactgtt tcagtttgtg
    16261 gttcaacacc tgattgtgca tcaagtactg taactgctcc gtcaagtaca cgtaatgaac
    16321 gttcaacttc tacagtgaag tctacgtgtc caggtgtatc gataatgttt acacggtgac
    16381 cttcccaagc tgctgttgtt gcagcagatg tgatagtaat accacggtct tgttcttgct
    16441 ccatccagtc catttgtgaa gcaccttcgt gtgtttcacc aattttgtgg atacggccag
    16501 tgtaataaag aatacgttca gtcgtagtcg ttttaccagc atcaatgtga gccatgatac
    16561 cgatattacg agttttttct aatgaaaatt ctctagccat gtattttttc tccttccagt
    16621 ttatattact gaataaatac gatagatatg gcgatgccct aagcatgaac gcttaatgaa
    16681 aattcattaa tatagaacac actcagggta aaagctttta tcttaccaac ggtagtgagc
    16741 aaatgctttg tttgcttcag ccattttgtg agtgtcctca cgtttcttaa cggcaccacc
    16801 tgtattattt gctgcatcta aaatttcgtt agctaaacga tcttccatcg ttttttcacc
    16861 acgaagacgt gcatagttaa ctaaccaacg taaacctaaa gtagtacgac gctctggacg
    16921 aacttctact ggtacttgat agttagaacc acctacacga cgagctttaa cttctaatac
    16981 tggcataatg ttgttgattg cttcttcgaa tacttctaat gcatcacgac cactgcgttg
    17041 ttcaactagg tcgaatgctg aataaagaat tctttgtgct gttccacgtt taccatctaa
    17101 cataattttg ttaattaatt tagttactaa cttagagtta tgaattggat ctggtaatac
    17161 gtctctttta ggtactgatc ctttacgagg cataatgtaa atcctccctt ccttatatta
    17221 aatattttaa gtttaagatt taattaaaaa ctaaattctt agtttttagg tttcttagtt
    17281 ccgtataatg aacgaccttg tctacgtccg tcaacacctg aagtatcaag tgctccacgt
    17341 acaatatggt aacgcacacc tggtaagtct tttacacgtc caccacgtac aagtacaaca
    17401 ctgtgttctt gtaagttatg tccgatacca gggatgtatg cgttaatttc gatgttgttt
    17461 gataaacgca cacgtgcata tttacgtaac gctgagttag gttttttagg tgtcattgta
    17521 cctacacgag tacatacacc acgtttttgt ggtgagttta agtcagtaaa ttttttcttt
    17581 ttactgttga aacctttatt taaagctgga gaatctgatt ttttgatttt gctttgtctt
    17641 ggtttacgta ctaattggtt aatagttggc atgtgatatg tcctcctctc ttcacttttt
    17701 aatcccacac atccaggtgg ttcattttta ggttaaataa aatttagtaa gtaaaacact
    17761 tactaattct catttaatca atgctactat tgtcgcattg acgttaatac ctacatgttt
    17821 acccaaagca tgtttgcttt tgaaaaaaga tacaggtata tttttctgat tgatttggct
    17881 taacacgcga gtcattaaat atacttcaac gtcttcagca ataatcaaag atgtaacttg
    17941 atctttcttt aacgctttaa gcgtttcttt aagaccaact acaaaatgtt gtttgttaaa
    18001 gcgtgcaact ttttccttag acaataattt atcctccaaa gttctgcttg catcaacctt
    18061 taatatttta acattgaaca agagaattcg tcaacattaa atcaaataat ttacataaaa
    18121 ataacaagag gctaaagcat tagcctctgt tatatacttg ttattccgtt acttcagttt
    18181 gagattcaac ttctgcaact ggtttagctg ttttttcgta ttttacgtcg ctataacgtc
    18241 tcataccagt accagctgga attaacttac caataattac gttttcttta agacctaata
    18301 agtcatcacg cttaccttta attgctgcat ctgtaagaac tcttgttgtt tcttggaatg
    18361 atgctgcaga taagaaactt tctgtttcaa gtgatgcttt agtaatacca agtaatactg
    18421 gtttagctgt tgcaggacgc ttacggtgtt taaatgcttc tctatttgca tctgtaaagt
    18481 tatgaatatc aactaatgaa cctggtaata acttcgtatc acctgcttcg ataattctaa
    18541 ctttacgtaa catttgtcga accataacct caacgtgttt atcgtcgatt tctacacctt
    18601 gcatacggta aactttttgt acttctttta ataagtagct ttcagtcgcg tttaatccag
    18661 caacagataa gtaattctta ggttcaatag aaccttcagt taatacttca ccacgttgaa
    18721 ctggttgacc gatttctaca ataattcttg aagtacctga agcaaggtat gatcttgttt
    18781 cattagcacc tttaacaaca atttcttgtt gtctatcttt tgctaattta atatcttcta
    18841 cgacaccttc gatttccgta attaccgctt gacctttagg gttacgtgct tcgaaaatct
    18901 cttgaatacg aggaagacct tgtgtgatat cgctacctgc taccccacct gtatggaatg
    18961 tacgcattgt aagctgtgta cctggttcac cgatagattg ggctgcaatt gtaccaactg
    19021 cttcaccaac ttcaactttt tcaccagtag caaggttttt accgtaacat ttttcacaaa
    19081 caccatgtcg tgcgttacaa gtaaatgctg agcgaatata catttgttca ataccagcat
    19141 ctgtaatttt cttagcaatt tcaggtgtaa ttaattcatc aggacgaatg attatttcat
    19201 cagtttcagg atgacgaatt gtttctttag aataacgacc ttcaatacgt tcgataaatg
    19261 gttcaatcat ttctgtacct tctttaatat cagaaactaa taaacctcta tcagtaccac
    19321 agtcttcttc acgaacaata acatcttgtg ccacgtcaac aagacgacga gtaagatatc
    19381 ctgagtcagc tgttttaagt gctgtatcgg caagaccttt acgtgcaccg tgagttgaga
    19441 tgaagtattc aagtactgtt aaaccttcac ggaatgaaga tgtgattggt aattcgataa
    19501 tcttaccaga tggtgcggcc atcaatccac gcatacctgc taactgtgta aagttagatg
    19561 cgttaccacg ggcacctgaa tcactcatca tgaagattgg gttagtttta tcaagtgatt
    19621 gcatcaattc accttgaatt tgatcttttg catctgtcca aatttcaaca actgcattat
    19681 atctttcttc ttcagtgatt aaaccacggt tgaattgttt tgtaattctg tcgactaatt
    19741 tttcatgctc atcaagtatt tgttgcttat caggtaatac tacgatatca gcaacaccta
    19801 ctgtaatacc agctttagat gagaatttga atcctaagtc tttcatacgg tctaacatca
    19861 ttgatgtatc agtgatgcta aatctgttga atacttctgc aataatatta cctaagaatt
    19921 ttttgttgaa aggttcaatt aattcttcat tttcaaagta ttcttttaat ccaccttcac
    19981 ctaaagttgt aggatcgatg aaatatctgt ttggtgtctt tctttctaag ttttcttgcg
    20041 taggttcatt aatataagca aatgaatctg gaatgatttc attgaatata attttaccta
    20101 ctgacgtagc aagaatcttt ttgttttgtt cttcagtaaa tgttggatta ttgaacgaac
    20161 ttgcatgtac accaattcta gtgtgtaaat gtacaaagcc atttgcatat gcttttaata
    20221 cttcatttgt attattaaag attgcgcctg tatttactgc atcttttctt tctaaagtaa
    20281 ggtaatagtt accaagtacc atatcttgtg atggtgtaac tacaggttta ccatctttag
    20341 ggttcaagat gttttgtgct gctaacatca acattcttgc ttcagcttgt gcctcttttg
    20401 ataaaggaac gtgaaccgcc atttggtcac cgtcaaagtc agcattataa gctgttgtta
    20461 caagtggatg tagacgaatc gcacgacctt caactaaagt tggttcaaat gcttgaatac
    20521 caagtctatg aagtgttggt gcacggttaa gtaatacagg atgttctcta attacttctt
    20581 ccaatacgtc ccaaacttca tcatccatac gttcgatttt actcttcgca tttttaatgt
    20641 tagttgcaat ttcacgttga actaattctt tcattacgaa tggtttaaat agttcaagtg
    20701 ccatttcttt tggtaaacca cattggtaca ttttcaagct tggacctact gcaataactg
    20761 aacgtcctga atagtcgaca cgtttaccaa gtaagttttg acggaaacga ccttgtttac
    20821 cttttaacat atgagataaa gattttaatg gacggttacc tgggccagta actggacgac
    20881 cacgacgacc attatcaatt aaagcgtcaa cggcttcttg taacatacgt ttttcgtttt
    20941 gaacgatgat accaggtgca cctaaatcta ataaacgttt caaacgatta tttcgattaa
    21001 ttacacgacg gtataagtcg tttaagtcac ttgttgcaaa tcgtccacca tctaattgaa
    21061 ccattggacg aatttctggt gggatgattg gaagtacatc taaaatcatc catgaaggtt
    21121 tgttacctga attacggaat gattcaacaa cttctaaacg tttaattgca cgagtaagtc
    21181 tttgaccagt agctgattcc aactcatcgc gtaacaattt aagttcttcg tcaagatcaa
    21241 tctcttcaag taaatcttta ataccttctg cacccatttt tgcaacgaat tgacctgggt
    21301 atttatcata ataatctctg aattcagctt cagataataa agttttcttt tctaaaccag
    21361 ttggacctgg atctacaaca acataagaag caaagtaaat aacctcttct aatgctcttg
    21421 gtgacatgtc aagtaataat cccatacgac ttggtatacc tttgaaatac caaatgtgag
    21481 aaactggagc agcaagttca atgtgaccca ttctttcacg acgtacttta gatttagtta
    21541 cttcaactcc acatctgtca cagaccatgc ctttgtagcg aacacgtttg tatttaccac
    21601 aactacattc ccagtctttt gtaggtccga aaattctttc acagaataga ccatcttttt
    21661 caggttttaa tgtacggtag ttgattgttt caggtttttt aacttcacca aaagaccaag
    21721 aacggatttt ttcaggtgaa gccaatccta ttttcatata atggaaatta tttacatcaa
    21781 tcaaggagcc tacctccttc aatttagatt agctgtgcta tttgattgat ttgcatttat
    21841 ttaaaatggc ttaaagtact aaactgaatc atcaataaaa ttcagcttag tatctttttg
    21901 cctgttttgt aaattgcgta ttaatcagta acttcttttt gtgtttcagg agcatcattt
    21961 tgttgtaaat ctactttgcg ttctacaaca tcatcgtcat caacgtctgt catttcgatt
    22021 tcattatctt gctcatccat aacttttaca tctaaaccta aactttgtaa ttctttcatc
    22081 aatactcgga atgattctgg aacacttggt ctagagatgt tttcaccttt aacaatagcc
    22141 tcgtatgttt tcacacgtcc tactgtatca tcggatttgt aagttaagat ttcttgtaat
    22201 gtgtatgcag caccatatgc ttcaagtgcc catacctcca tctcaccgaa acgttgtcca
    22261 ccgaattgcg ctttaccgcc aagtggttgt tgtgtaacaa gtgaatatgg tcctgttgaa
    22321 cgcgcatgta atttatcatc aaccatgtgc gcaagtttca acatgtacat tacacctact
    22381 gaaatacggt tatcgaatgg ttcacctgta cgtccatcat aaagtacagt tttaccatca
    22441 cgagccatac cagcttcttc aattgttgac catacatcgt catcgtttgc accgtcaaat
    22501 actggtgatg caacgtgaat accaagattt ttagcagcca tacctaagtg tagctctaat
    22561 acttgtccga tgttcatacg agatggtaca ccaagaggat ttaacatgat atcgatcgga
    22621 cgtccatctg gtaagtaagg catatcttct tcaggaacaa tcttagaaat gacacctttg
    22681 ttaccatgtc gaccacacat cttatcacca acatgaattt tacgtttttg aacgatatat
    22741 acacgtacta attggtttac accaggtgat aatgtatcgt cgccttcttc acgattgaat
    22801 acttttacat caagaacgat accgccagcg ccgtgaggta cacgtaatga agtatctcta
    22861 acttcacgtg ctttttcacc aaagattgca tgtaacaatc tttcttcggc agttaactca
    22921 gttacacctt taggcgttac tttaccaact aaaatatctc catcttttac ttctgcacca
    22981 atataaacga taccacgatc gtctaagttc ttaagtgcac tttcagaaac attaggaata
    23041 tctcttgtga tttcttcagg tcctaactta gtatcacgtg cttctgattc atactcttca
    23101 atatgaatag aagtatacac gtcatctttc acaagtcttt cactcatgat aacggcatcc
    23161 tcatagttgt aaccgtccca agtcatgaaa ccaactacta cgtttctacc taatgccatt
    23221 tctcctaatt ccatagatgg tccatctgct aaaatctcgt tatactcaac aacatctcca
    23281 actgcaacga ttggacgttg gttgtaacat gtacctgagt ttgaacgttt aaatttagct
    23341 aatggatagc gatctaattc accttcatgc tcaacgccgt tctcttcaac tagacgacgt
    23401 acaagaattt cattagattc aacatgttca acacgacctc tgtgcttagc tgtaatagct
    23461 gcaccagaat cacgtgctgc aacgtgttcc atacctgttc caacaaatgg tgcttctgga
    23521 ttcatcaaag gcactgcttg acgttgcatg ttcgcaccca tcaatgcacg gtttgagtca
    23581 tcgttttcta agaatggaat acatgctgtc gctgctgaaa caacttgctt cggcgataca
    23641 tccatataat ccattttttc tttagccata actgtattgt taccacggaa acgacataca
    23701 acttcatcat ccatgaaacg accattttca tctaatttag agtttgcttg tgctacaaca
    23761 tagctatctt cttcgtcagc tgttaaatag tcaatttgat cagtgatagc atgtgtatct
    23821 aaatcaactt tacgatatgg tgtttcaata aagccgaatt catttacacg tgcataactt
    23881 gataatgagt taatcaatcc aatgtttggt ccctcaggtg tttcaattgg acacatacgg
    23941 ccatagtgag agtagtgaac gtcacgtact tccatttgag cacgttcacg tgttaaacca
    24001 ccaggtccta atgctgatag acgacgttta tgcgttaact cagctaatgg gtttgcttgg
    24061 tccatgaatt gtgataattg agagctacca aagaattctt taatagatgc aataacaggt
    24121 cgaatattaa ttaattgttg aggtgtgata gactcagtat cttgaattga cattctttca
    24181 cgtacaactc tttccattct tgataaaccg atacggaatt ggttttgtag taattcacct
    24241 acagaacgta aacgacggtt acctaaatgg tcaatatcat ctgtatatcc aataccgctt
    24301 aataagttaa agaagtaact cattgaagca atgatatctg ctggtgtaat gcatttaact
    24361 tctgagtcag ggaaagcatt accaattaca gttgtcgtac gaccttcatc atcgttagga
    24421 acatatactt taattgattg aatttctact ggctcgtcta taacgctacc atgcaattca
    24481 aacacttcgc tgtttgcatt tgattcaagt acatccatga tttcgtcgat tttacgacga
    24541 tcaagcactg taccttcttc aactacaatt tcaccagttt cagtatttac aattggctca
    24601 gctaattttt gattaaataa acgatgtttt aaatgtaatt ttttgtttgt tttataacga
    24661 cccacgcttg ctaagtcata gcgttttgga tcaaagaaac gtgaatacaa tagactttta
    24721 gcattttcaa cagttggtgg ttcacctgga cgtaaacgtt catagatttc taataacgct
    24781 tgttcagtgt tttcagtgcc gtctttctct aaagtattac gtaaatattc attgtcacct
    24841 aaaaggtcaa caatttcttg gtcacttgag aaacctaatg cacgtaacaa tactgttaat
    24901 ggtagtttac gtgttctatc aatacgtacg tatacaacat ctttagcatc tgtttcatat
    24961 tctaaccatg caccacggtt tggaataatt gttgcatcat agttttcacg accatttttg
    25021 tcgatttttt cattgaaata aacggatggt gaacgaacta attgagatac gattacacgt
    25081 tctgcaccat tgataacgaa cgtacctgta tcagtcatta atgggaaatc acccataaag
    25141 acttcttgtt ctttaacttc tcctgtttct ttaatgatta gacgcacttt tacacgaaga
    25201 ggtgcagcat aagtagcgtc acggttttta gattcttcta aatcatattt tggttctcct
    25261 aaacggtaat ccacaaactc taatgacaaa ttaccagtaa aatcttcaat tggagaaatg
    25321 tctctaaaca tttcgattaa accttctctt aggaaccact cgtaagattt agtttgaatt
    25381 tctattaagt ttggtaattc taatacttct gaaattctcg cgtagtttct acgtttacga
    25441 tgtcttccat attggacaac ttgacctgcc aaacagattc acccctcaaa aattatgtga
    25501 tccgctttta gctaaatacc ataagcatat taataaaaag acaaaaagaa aacggcaaca
    25561 ctaatgatga caccattttc gattctatat ttatt
//
LOCUS       DAOBBH010000018        25410 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.018, whole genome shotgun sequence.
ACCESSION   DAOBBH010000018 DAOBBH010000000
VERSION     DAOBBH010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25410
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.018"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            1..1714
                     /locus_tag="P6L01_000517"
                     /pseudo
     CDS             1..1714
                     /locus_tag="P6L01_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498636.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
     gene            complement(1812..2167)
                     /locus_tag="P6L01_000518"
                     /pseudo
     CDS             complement(1812..2167)
                     /locus_tag="P6L01_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
     gene            complement(2472..2560)
                     /locus_tag="P6L01_000519"
                     /pseudo
     CDS             complement(2472..2560)
                     /locus_tag="P6L01_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2658..3413)
                     /locus_tag="P6L01_000520"
     CDS             complement(2658..3413)
                     /locus_tag="P6L01_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDP3029611.1"
                     /translation="MDIVNIVIMLLIGVFGGFISGLVGVGGAIIIYPAILLLPPLFGA
                     PAYSAYIASGLTSSQVFFSTLSGSLKARKKTEFSPQLVLYMGGGMIIGSMLGAFLANL
                     FDATFVNTVYIIIALLALTLMFIKVKPSSEKSSFNKYLLVIIGLFIGIISGIVGAGGA
                     FIIIPILLVLFKLPMNTVVANSIVIAFISSIGAFVIKLIQGYIPLYDALFLIIGSIIF
                     APIGLKLSKKVPNIIQKWIISILIVFAIVQLIL"
     gene            complement(3413..3673)
                     /gene="cstR"
                     /locus_tag="P6L01_000521"
     CDS             complement(3413..3673)
                     /gene="cstR"
                     /locus_tag="P6L01_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide-sensing transcriptional repressor
                     CstR"
                     /protein_id="HDP3029612.1"
                     /translation="MNYDKKMINRINRIQGQLNGIIKMMEEGKDCKDVITQISASKSS
                     LQRLMGIIISENLIECVKVAADDEESSQELINEAVNLLVKSK"
     gene            3810..4874
                     /gene="cstA"
                     /locus_tag="P6L01_000522"
     CDS             3810..4874
                     /gene="cstA"
                     /locus_tag="P6L01_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide response sulfurtransferase CstA"
                     /protein_id="HDP3029613.1"
                     /translation="MKQYGEKFIDEFSKAELEKLAKQGQLIDVRTEEEYALGHINGSI
                     LHPVDEIESFNKEKNKTYYVICRSGNRSANASKYLAKQGYNVINLDGGYKAYEENDSY
                     DTQEEYKSIEIKADRKQFNYRGFQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSDI
                     KSWVKQTRHTLVKLDENNNGINAIIQKEKAKDLDINYSAKGTTIVLFSGELDKAVAAL
                     IIANGARAAGKDVTIFFTFWGLNALKKVQTVNVKKQGIAKMFDLMLPQKNIRMPLSKM
                     NMFGLGNMMMRYVMKKKNVDSLPTLINQAIEQNIKLIACTMSMDVMGIQKEELRDEVE
                     YGGVGTYIGATENANHNLFI"
     gene            4905..6239
                     /gene="cstB"
                     /locus_tag="P6L01_000523"
     CDS             4905..6239
                     /gene="cstB"
                     /locus_tag="P6L01_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
                     /protein_id="HDP3029614.1"
                     /translation="MFFKQFYDNHLSQASYLVGCQRTGEAIIIDPVRDLSKYIEVADS
                     EGLTITQATETHIHADFASGIRDVAKRLNANIYVSGEGEDALGYKNMPSKTQFVKHGD
                     IIQVGNVKLEVLHTPGHTPESISFLLTDLGGGSSVPMGLFSGDFIFVGDIGRPDLLEK
                     SVQIKGSTEISAKQMYESVQNIKNLPDYVQIWPGHGAGSPCGKALGAIPISTIGYEKI
                     NNWAFNEIDETKFIESLTSNQPAPPHHFAQMKQVNQFGMNLYQSYDVYPSLDNKRVAF
                     DLRSKEAFHGGHTKGTINIPYNKNFINQIGWYLDFEKDIDLIGDKSTVEKAKHTLQLI
                     GFDKVAGYRLPKSGISTQSVHSADMTGKEEHVLDVRNDEEWNNGHLDQAVNIPHGKLL
                     NENIPFNKEDKIYVHCQSGVRSSIAVGILESKGFENVVNIREGYQDFPESLK"
     gene            6258..7451
                     /gene="sqr"
                     /locus_tag="P6L01_000524"
     CDS             6258..7451
                     /gene="sqr"
                     /locus_tag="P6L01_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II sulfide:quinone oxidoreductase Sqr"
                     /protein_id="HDP3029615.1"
                     /translation="MNKHYQIVIIGGGTAGVTVASRLLRKNQNLKEKIAIIDPADHHY
                     YQPLWTLVGAGVSSLKSSRKDMESVIPEGANWIKQAVSSFQPENNSVILGDNTVVYYD
                     FLVVAPGLQINWSSIKGLKENIGKNGVCSNYSPDYVNETWNQISNFKQGNAIFTHPNT
                     PIKCGGAPMKIMYLAEDYFRKHKIRSNANVIYATPKDALFDVGKYNKELERIVEERNI
                     TVNYNYNLVEIDGDKKVATFEHIKAYDRKTISYDMLHVTPPMGPLDVVKESTLSDSEG
                     WVDVNPTTLQHKSYSNVFALGDASNVPTSKTGAAIRKQAPIVANNLLQVMNNQMLTHH
                     YDGYTSCPIVTGYNRLILAEFDYNKNTKETMPFNQAKERRSMYIFKKDLLPKMYWYGM
                     LKGLI"
     gene            complement(8117..9103)
                     /locus_tag="P6L01_000525"
     CDS             complement(8117..9103)
                     /locus_tag="P6L01_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDP3029616.1"
                     /translation="MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEF
                     TNTESFCHPEGIHSVRGRLTFSEDEHPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLN
                     MGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
                     VFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGL
                     ELAEKYGIDGVMIGRGIFHNPFAFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQF
                     KSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDVKIEL"
     gene            9412..9615
                     /locus_tag="P6L01_000526"
     CDS             9412..9615
                     /locus_tag="P6L01_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029617.1"
                     /translation="MEDLKESLKSLGWWDLFFAIPMFLLFAYLPNYNFITIFLNIVII
                     IFFSIGLILTTHIIIDKIKSNTK"
     gene            9664..9960
                     /locus_tag="P6L01_000527"
     CDS             9664..9960
                     /locus_tag="P6L01_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HDP3029618.1"
                     /translation="MATKKDVHDLFLNHVNSNAVKTRKMMGEYIIYYDSVVIGGLYDN
                     RLLVKATKSVRHQLQDNTLVSPYPGAKEMILIPDITEAINLTNLFELIKNDLKN"
     gene            10168..10770
                     /locus_tag="P6L01_000528"
     CDS             10168..10770
                     /locus_tag="P6L01_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029619.1"
                     /translation="MLISLVIPVFALLVIGGIIWVIIEGIVHISKKNKAIDNFLNQVN
                     KVSETYKFATAFLFLILATAGISQFYLYYIVSAFLFWLVILTFGIAGIIFLMPYGLCF
                     LPLYKQKKKKQTFKKYMVYTTIGLSICLGLSLVLVHTTKIYMDEGGVRYYYGSFVMKQ
                     AGGYAYLALAVLSTLLIVAKKATNKNKEIETVDNTNITER"
     gene            11037..14189
                     /locus_tag="P6L01_000529"
     CDS             11037..14189
                     /locus_tag="P6L01_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA2/NAM7 family helicase"
                     /protein_id="HDP3029620.1"
                     /translation="MSQLLNDTLSAWLLIESLSPGEVNFTAEDILSAEHFKNGAKQAQ
                     LQSFDEYFEIWNSERFIISEEKSETGELIFKFYRHCFRYNEINLKIQDIFDDYSDIHN
                     PNGTHCYGYTFNTDKHGKVIVDSIHIPMIMSALKEIEKNKNANIEEKFNDSVEKFFQK
                     VKEILADEPINEFKLKKMDKAYDEYFSVLNSKKDGLFGHYVAIEYVKDSDLPQPEFNS
                     FFISDIEKARKSPNQTLIDYIEGVEESQRIEVDENKEMFDKFLHPSRLPDGRWPSQTE
                     FRLSLMQQLAVNQITSGNERISSVNGPPGTGKTTLLKDIFAHLVVERGKELAKLNNPK
                     DAFVKTKIHETDDKYVYLLKESIAKYKMVVASSNNGAVENISKDLPKIEEIIRNPEKC
                     KFPKYEQNYANLAHELKDFAEIAEDLIGESAWGLFSGVFGKSTNINQVLSHMLKQDAN
                     DIGFAKLLQNENNRMSYNELMSEWQSHQRAFLEELRHVEMLKEESIRAYDVYKNCESF
                     SKIEQVINSEKTSIEEQVYHLDNETLRDNKEIEDLDNRINYIVKQIETLNELIKSIKE
                     SNKGFINKLKAMFNSEEDESYKDHNKEKQQLLTQQLELEKCKKNKHEDLVSKLKEKEK
                     LIKQLTKVQLQLDELNSQLQELEAYRIESKITIPEKDFWSDNNYDERQVTNLWTSDEL
                     QYRRAMLFLRAMILHKLLLIANNTTIYYAINDFKDRRKLIDANPDKVHNAWNVMHLIF
                     PVVSTTFASFKSMYGGIPKDFIDYLFIDEAGQAIPQAAVGALYRSKKVVAVGDPIQIE
                     PVVTLESHLIDNIRKNYHVPEYLVSKEASVQSVADNANQYGFWKSDATDSNQKTWIGI
                     PLWVHRRCLKPMFTIANQIAYNNKMVLPSNITKVGKTGWYDVKGNAVQKQFVKEHGEK
                     VVGLLADDWIEAIKEGKNEPSSFVISPFSAVQQQIKRMLKQQLPTRIDIERTKINQWV
                     DKSIGTVHTFQGKEAQKVYFVIGTDNTQDGAVNWSCEKPNLLNVAVTRAKKEFYVIGD
                     MQRIQMKPFYETIFKERNVK"
     gene            14246..14728
                     /locus_tag="P6L01_000530"
     CDS             14246..14728
                     /locus_tag="P6L01_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029621.1"
                     /translation="MEKFNNWILNAISGSQTDKNETTEELKGAKFIILYAYSMLVLLA
                     LVISNIFIHILEPKLSITTQIIIVLILIEALIGLRFLKAYDVKRGKDKENKKNSKDFV
                     KLKSILVAILFTSLALTAGTVADIYGFTDLGNTRSDLIVWSIGGIIFGLVCYTMEDKK
                     "
     gene            14934..15920
                     /locus_tag="P6L01_000531"
     CDS             14934..15920
                     /locus_tag="P6L01_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C"
                     /protein_id="HDP3029622.1"
                     /translation="MKKCIKTLFLSIILVVMGGWYHSAHASDSLSKSPENWMSKLDES
                     KHLTEINMPGSHDSGSFTLTDPVKSVWAKTQGKDYLTQMKSGVRFFDIRGRASADNMI
                     SVHHGMVYLHHELGKFLDDAKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYY
                     YNNPQYQNLFYTGSNANPTLKETKGKIVLFNRMGGTYIKSGYGADTSGIQWADNATFE
                     TKINNGSLNLKVQDEYKDYYDKKVEAVKNLLAKAKTDSNKDNVYVNFLSVASGGSAFN
                     STYNYASHINPEIAKTIKENGKARTGWLIVDYAGYPWPGYDDIVSEIIDSNK"
     gene            16141..16911
                     /locus_tag="P6L01_000532"
     CDS             16141..16911
                     /locus_tag="P6L01_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDP3029623.1"
                     /translation="MMKRLNKLVLGIIFLFLVISITAGCGIGKEAEVKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIINSEMVIQPNNEDMVAKGMVLYMNRNTKTT
                     NGYYYVDVTKDEDEGKPHDNEKRYPVKMVDNKIIPTKEIKDKNIKKEIENFKFFVQYG
                     NFKDLSKYKDGDISYNPEVPSYSAKYQVTNDDYNVKQLRKRYDIPTNKAPKLLLKGTG
                     NLKGSSVGYKDIEFTFVEKKGENIYFSDSLHLEPSEDK"
     gene            16963..17733
                     /locus_tag="P6L01_000533"
     CDS             16963..17733
                     /locus_tag="P6L01_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDP3029624.1"
                     /translation="MIKRVNKLVLGISFLFLIISIFAGCGTGKEAEIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIVRSSMSIQSNGKDMNIKGMVLYMNRNTRTT
                     NGYYYVDVIEREDKGIHRDNEKRYPVKMVDNKIIPTKEIKDEKIKKEIENFKFFVQYG
                     DFKDLSKYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYNIPTNKAPKLLLKGTG
                     NLKGSSIGYKKIEFTFVEKKGENIYFSDGLHFNPSEDK"
     gene            17798..18568
                     /locus_tag="P6L01_000534"
     CDS             17798..18568
                     /locus_tag="P6L01_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDP3029625.1"
                     /translation="MMKRLNTLVLYISFLILIISIVAGCGTGKEAEIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDEFDKNDKGTWTISSEMAIQKKGEALNIKGMVLKLNRNTRSA
                     KGFYYVNAIKKDENGRPQDKQIEYPVKMIDNKIIPTKDIKDEKIKKEIENFKFFAQYG
                     NFKDLTKYKGGDISYNPEAPIYSAKYQLTNDDYNVKQLRKRYDIPTNKAPKLLLKGSG
                     NLDGSSIGYKKIEFTFVEKKGENTYFTANLHFKPSNDE"
     gene            18633..19403
                     /locus_tag="P6L01_000535"
     CDS             18633..19403
                     /locus_tag="P6L01_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDP3029626.1"
                     /translation="MMKRLNKLVLGISFLFLVISITAGCGMGKEAEIKKSFEKTMSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIVNSQMAIQNKGEPMKSKGMVLYMNRNTRTT
                     NGYYYVNVIKGEDKGKHQDNEKRYPVKMVDNKIILTKEIKDENIKIEIENFKFFVQYG
                     NFKDLENYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYDIPTNKAPKLLLKGTG
                     NLKGSSVGYKDIEFTFVEKKEENIYFSDGLIFKPSEDK"
     gene            19470..20237
                     /locus_tag="P6L01_000536"
     CDS             19470..20237
                     /locus_tag="P6L01_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDP3029627.1"
                     /translation="MKRLNKLVLYISFLILVISFTAGCGMGKEAEIKKSFEKTLSMYP
                     IKNLEDLYDKEGYRDDQFDKNDKGTWIINSEMVVQPKGERMKSKGMVLYMNRNTKTTT
                     GKYIVSETLHDEDGRPKSKDKEYPVKMVDNKIIPTKGIKDENIKKEIENFKFFAQYGS
                     FKDLSKYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYKIPTNKAPKLLLKGSGD
                     LKGSSVGYKDIEFTFVEKKGENTFFTDSLHLEPSEDK"
     gene            20334..22571
                     /locus_tag="P6L01_000537"
     CDS             20334..22571
                     /locus_tag="P6L01_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDP3029628.1"
                     /translation="MQRDYLIRVETESMPDFKRLNGLMIGFVIKGEAHIYDENNMTQC
                     NSGDIFIINHRDLYRFQLQQDGIICYIQFQMKYLADKFDDAHCLYFHLTDATTTKNIH
                     QLRNIMARLVSTHIRHNELSKLTEQQLVIQLLMHMIHYVPRTYHSNQSILNDDKVNQV
                     CDYIELHFHEDLSLSELSEYVGWSESHLSKKFTESLGVGFQHFLNTTRIEHAKLDLTY
                     TDETITDIALQNGFSSAASFARTFKHFTHQTPKQYRGDRPAITENQQSAQHNYHDREL
                     ILLLNDYIEEMNHFIEDIEKMNYKEIAFKPTNQQLNQFNHIIQVGYLRNLLNTQYQSQ
                     LLTCHHDFQVNEVLAYDVMPYIMKKLNAPFTYDAEISNIFYDIDLCLDFLLDHNFSLT
                     MHLNQYDSRDYIDAFKVFIHHVALHVSHRKDLKFNLYVTTLHTSLIEMIDYFKALFPN
                     GGLYIHLDQATERHLPLLKRLEPHINHFVFDANSNDAVDFNKMNDDEFKTASQMIINK
                     TNYLIDLMHRHNLKRPLILLNWNTLTGDTFITNGEYFRGGIIIEQLLKLSTKVEGIGY
                     WLNYDLHVSHCRNERDYMNSIELFHQYNGKRPVYFTALLFNKLTSNILYSDDTCIVTG
                     TDSNFQILLYDAKHFNPYLALDNQMNMRATEMIHLNINALEDGMYKIKHFTLDKENGA
                     LFNLWRKHHTIHGMDKDSIDYVNRMSFPKLEVYDIDITDTLALNIKMITNGIHLIEVK
                     RYPSS"
     gene            22722..23900
                     /locus_tag="P6L01_000538"
     CDS             22722..23900
                     /locus_tag="P6L01_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDP3029629.1"
                     /translation="MNQQLIEALKSKEDKMIEIRRYLHQHPELSFHEDETAKYIAEFY
                     KGKDVEVETNVGPRGIKVTIDSGKPGKTLAIRADFDALPITEDTGLSFASQNKGVMHA
                     CGHDAHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKAMIENGVLDGVDHV
                     LGVHVMSTMKTGNVYYRPGYVQTGRAFFKLKVQGKGGHGSSPHMANDAIVAGSYFVTA
                     LQTVVSRRLSPFETGVVTIGSFDGKGQFNVIKDVVEIEGDVRGLTDATKATIEKEIKR
                     LSKGLEDMYGVTCTLEYNDDYPALYNDPEFTEYVAKTLKEANLDFGVEMCEPQPPSED
                     FAYYAKERPSAFIYTGAAVEDGEIYPHHHPKFNISEKSLLISAEAVGTVVLDYLKGDN
                     "
     gene            23902..25290
                     /locus_tag="P6L01_000539"
     CDS             23902..25290
                     /locus_tag="P6L01_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDP3029630.1"
                     /translation="MNETYRGGNKLILGIVLGVITFWLFAQSLVNVVPNLQQSFGADM
                     GTISIAVSLTALFSGMFVVGAGGLADKIGRVKMTNIGLLLSIIGSALIIITNLPALLI
                     LGRVIQGVSAACIMPSTLAIMKTYYQGAERQRALSYWSIGSWGGSGICSLFGGAVATT
                     MGWRWIFIFSIIVAVLSMLLIKGTPETKSEITNTHKFDVAGLIVLVVMLLSLNVVITK
                     GAALGYTSLWFFGLIAIVIVAFFIFLNVEKKVDNPLIDFKLFENKPYTGATISNFLLN
                     GVAGTLIVANTFVQQGLGYTALQAGYLSITYLIMVLLMIRVGEKLLQKMGSKRPMLLG
                     TFIVVIGIALISLVFLPGIFYVISCVVGYLCFGLGLGIYATPSTDTAISNAPLDKVGV
                     ASGIYKMASSLGGAFGVAISGAVYVGAVAATSIHTGAMIALWVNVLMGIMAFIAILFA
                     IPNDDKRVKDAK"
BASE COUNT         9435 a         3461 c         4557 g         7957 t
ORIGIN      
        1 aattttcagg aaacaaatac aaatttttta gagaatctag aatctttaaa tgatgacaat
       61 tatgaactgc ttaatgataa agaacttgtt agtgattcaa atgaattaaa attaattagt
      121 aaagtttata tacgtaaaaa agacaaaaaa ctattagatt ggcaattatt aataaagaat
      181 gtatacctag atactgaaga agatgacaat ttattttcag aatccggtca tcattttgat
      241 gcaatattat ttctcaaaga agatactaca ttacaaaata atgtatatat tatacctttt
      301 ggacaagcat atcatgatat aaataatttg attgattatg acttcggaat tgattttgca
      361 gaaagagcaa tcaaaaatga agacatagtt aataaaaatg ttaatttttt tcaacaaaac
      421 aggcttaaag agattgttaa ttatagaagg aatagtgtag attacgttag accttcagaa
      481 tcttatatat cagtccaagg acatccacag aatcctcaaa tttttggaaa aacgatgact
      541 tgtggtacaa gtatttcatt gcgtgtaccg aatagaaagc agcaattcat agataaaatt
      601 agtgtgataa tcaaagaaat aaacgctatt attaatcttc ctcaaaaaat tagtgaattt
      661 cctagaatag taactttaaa agacttgaat aaaatagaag tattagatac tttattgcta
      721 aaaaaactat cgaattcttc aactacagaa aatatatcta tagatatatc aagattttta
      781 gaactaagta atatgatact cttgttagat gatatgctcg acgtcaatat atataaattc
      841 ttttaaaaat aatacattag agacatttga tgcgactgat cctgaagttg attacatcac
      901 tgaaatagga gattacttat taaaatatga cgttaattct ataaatgatg tcagaattga
      961 agtgatagat aatttggggc attcaaataa tatgctacta aaaacgatac tacatgcaga
     1021 agttgaaatg gaagatggaa agaaatattt attacaaaat gggaaatggg gttattttaa
     1081 tagagaattt tttgaccttt tgaatgatca tttgaatgag atagaaatta ggtataacac
     1141 actaactccc acaggtttag tatttaaaga aggagaagaa ggatatataa aagaaatagt
     1201 aggaagattg ccagaagaat atttaatgtt acataaaaaa ttcataaaac caataaataa
     1261 gaattttata gtaaaaggaa atggaataga gttggcagac ttatacaata ttaaaaacaa
     1321 agagcttttc acgattaaaa gaggaattaa tacatcttta tctctttata gtctagaaca
     1381 gaatataata gcaattaacg ccttaaaata tccagaatca tataattttg aagaattaat
     1441 agaagctatt cctgataact cggaaaatat atttaatgat atacaacgga gtacaaattt
     1501 tagtatagta tggattttac cgatatcatc tattgataat atgcccataa aagatatggt
     1561 tcatactagt aacgtaataa ataaaaactt tcaattaact aatttaggtt cagtattact
     1621 taaaaataaa ttagtagagt ggtctctcta tttaaaagat caaagaatta atccaattat
     1681 ttatatggaa acgccaactg aagatagaaa ttaaactttt attttaaatc accctagttc
     1741 atacctaaaa gactttcaca caaacaaaca aaggaggaaa cttaaattcc tcctttccct
     1801 tattactcat actataattc aattttaacg tcttcgtcca tttgggcttc aaattcatct
     1861 agtaatgctc gtgcttctgc aattgattgt gtgttcatca attgatgtcg aagttcgcta
     1921 gcgctcttat gccacgcaca tagattttaa agaatctacg caaactcttg aattgtcgta
     1981 tttcatcttt ttcatatttg ttaaacaatg ataaatgcaa tctcaacaga tctaatagtt
     2041 ccttgcttgt gtgttcgcgt ggttcttttt caaaagcgaa tggattatgg aaaatgccgc
     2101 taccaatcat gacgccatca atgccatatt tttctgcaag ttcaagtcct gtttttctat
     2161 cgggaatatg ggagtgggac agaaatgata ttttcccaaa atttatttcg tcgtcccact
     2221 ccaacttgca cattattgta agctgacttt ccgccagcta ctatgttggg gcccctgact
     2281 agaattgaaa aaacttgtta caagtgcatt ttcgttcagt caactactgc caatataact
     2341 ttgtagagca tagaacattg atttatgtcc caacctgcaa tttcgtcacg taaattttta
     2401 atagcttcga ttaattccca ctgtgcatct actttactaa tgtgtttgat aaaaacttaa
     2461 ataattttaa ttcagtcatc agtggcatta aatcttctat catttttagt tatagttgat
     2521 aaatttatat ttataagcat atatatggat atttcatcaa gaatttttaa tcatataaat
     2581 ccgaactgca tacatatttg ttgaaataag aggtattatt tttcgggaaa ttgctgtctg
     2641 agttataagt attagtttta taaaatgagt tgaactatag caaaaacgat taaaatactg
     2701 ataatccatt tttgtattat gttagggact tttttactta attttaaccc tattggagca
     2761 aatataatac tccctattat aaggaataag gcgtcatata aagggatata accttgaata
     2821 agtttgatga caaaagcacc aattgaagat ataaaagcaa ttactatact attagcgact
     2881 acagtattca ttggtaattt gaataaaacc aataatatag gaataataat gaaggcacca
     2941 cctgcaccta ctatacctga aataatacca atgaaaaggc caatgataac taataaatat
     3001 ttattaaatg aagacttttc ggaactaggt ttcactttaa taaacattaa tgttaatgca
     3061 agtaaagcaa taatgatata taccgtattt acaaatgtag catcaaataa atttgctaga
     3121 aatgcaccta acatactccc tataatcatg ccgccaccca tataaagaac taattgtggt
     3181 gagaactctg ttttttttcg agcttttaat gagccactta atgtactgaa aaagacttgg
     3241 ctagaagtaa gacctgatgc gatatatgcg ctatatgcag gggctccgaa taatggtggt
     3301 aataataaaa tagctggata aataatgata gcaccaccta cgcctactag accagatatg
     3361 aacccaccga ataccccaat gagtaacatg ataactatat taacaatatc cattacttac
     3421 ttttcaccaa taagtttaca gcttcattaa ttaactcttg ggagctttct tcatcatccg
     3481 cagctacttt tacacattct attaaattct cactaataat gatacccatc aagcgttgga
     3541 gtgaactctt tgatgcactt atttgtgtaa tgacatcttt acagtctttt ccttcctcca
     3601 tcattttaat aattccattt agttgccctt gtattctatt aatacgatta atcatttttt
     3661 tatcataatt catagtcata cctccacttt taattgaata aaaatatatt aatagataaa
     3721 cacaaatgtg tcaaataccc ctagaggtat ttgacaagtt ccattcaacc gtttagaata
     3781 cccctacagg tatttttagg gaggttatta tgaaacaata cggagaaaag tttatcgatg
     3841 aatttagtaa agcagaattg gaaaaactag ccaagcaagg gcaattaatt gatgttagaa
     3901 cagaagagga gtatgcatta ggacatatca atggttccat acttcatcct gttgatgaga
     3961 ttgagtcatt caataaagaa aaaaataaaa cctattatgt aatctgtaga agtggtaaca
     4021 gaagtgctaa tgctagtaaa tatttagcta aacaaggtta taacgttata aatcttgatg
     4081 gtggttataa agcttatgaa gaaaacgata gttatgatac acaagaagaa tataaaagta
     4141 tagaaattaa agcagatcgt aaacaattta actatcgtgg ttttcaatgt ccagggccaa
     4201 ttgtaaaaat tagtcaagaa atgaagaata ttgaagtagg tgaccaaatt gaagtcaaag
     4261 tcacagaccc tggattccct agtgatatta aaagttgggt gaaacaaaca aggcatactt
     4321 tagttaagct tgatgaaaat aacaatggaa ttaatgcgat tattcaaaaa gaaaaagcaa
     4381 aagatttaga tataaattat tctgctaaag gtactacaat tgtattattt agtggagaat
     4441 tagacaaagc tgtagcagca ttgattattg caaatggcgc tagagctgct ggaaaagatg
     4501 taactatctt ctttactttt tgggggctta atgcattaaa aaaagtgcaa acagttaatg
     4561 ttaaaaagca aggtattgca aaaatgtttg atttaatgtt gccccaaaag aatatacgaa
     4621 tgcctctttc caaaatgaat atgtttggtt taggaaatat gatgatgcgc tacgtaatga
     4681 aaaagaaaaa tgttgattca ttaccaacac ttatcaatca agctattgag caaaatatca
     4741 aattaatcgc ttgtacgatg agtatggatg tcatgggtat tcagaaagaa gaacttagag
     4801 atgaagttga gtacggtggt gtaggcactt atattggtgc tactgaaaat gcgaatcata
     4861 atttatttat ctaattaaat ctattaataa aaggagttgt tatcatgttt tttaaacagt
     4921 tttacgataa tcatttatct caagcatcat atttagtggg ttgtcaacgt acaggagagg
     4981 caataataat agaccctgtt cgtgatttat cgaaatatat agaagttgca gattctgaag
     5041 gtttaacaat tacacaagct acagaaacac atattcatgc tgattttgct tcaggaattc
     5101 gtgatgtggc taaacgctta aatgcaaata tatatgtgtc tggcgaaggt gaagatgcat
     5161 tagggtataa aaatatgcca tcaaaaacac aatttgttaa acatggagat atcattcaag
     5221 taggcaatgt taaattagaa gttctgcata ctccaggaca cacgcctgaa agtattagct
     5281 ttttactcac tgatttaggt ggtggttcaa gtgttccgat gggattattt agtggtgact
     5341 ttatttttgt tggtgatata ggtagacctg atttattaga aaaatctgtt caaataaagg
     5401 gttctacaga aattagcgcg aaacaaatgt atgagtccgt tcaaaatatt aaaaatttac
     5461 cagactatgt tcaaatctgg ccgggtcatg gtgctggaag cccttgtggt aaagcattag
     5521 gtgccatacc tatatctaca ataggttatg agaaaattaa taactgggca tttaatgaaa
     5581 ttgatgagac taaatttatt gaatcattaa catcaaatca accagcacca ccgcatcatt
     5641 ttgcacaaat gaaacaagtt aatcagtttg gtatgaattt atatcaatca tatgatgttt
     5701 atcctagttt agataataag agagtagcat ttgatcttcg tagcaaagag gcctttcacg
     5761 gtggccacac aaaaggaaca atcaatatac catacaacaa aaactttatt aatcaaattg
     5821 gttggtactt agattttgaa aaagatatag atttaattgg agataaatct actgttgaga
     5881 aagcgaaaca cactttacaa ttaattgggt ttgataaggt agcaggctat cgtttgccaa
     5941 aatcaggcat ttcaacccag tccgttcata gcgctgatat gacaggtaaa gaagaacatg
     6001 tattagacgt acgtaatgat gaagagtgga ataatggaca cttagatcaa gcagttaata
     6061 ttccacatgg taaattatta aatgaaaata ttccttttaa taaagaggat aaaatatatg
     6121 tacattgtca gtcaggtgtt agaagttcaa ttgcagtggg tatattggaa agcaaaggtt
     6181 ttgaaaatgt ggtgaatatt agagaaggct atcaagattt tccagaatca ttaaaataat
     6241 ttaaggatgt ggaaaaaatg aataagcatt atcaaattgt tattattggt ggcggtacag
     6301 caggtgttac cgtagcatca agactattaa gaaaaaatca aaacttaaaa gagaaaatag
     6361 caattataga cccagcagac catcattact atcaaccatt atggacgttg gttggtgcag
     6421 gggtatctag tttgaaaagt tctcgtaaag atatggaaag tgttatacct gaaggtgcta
     6481 actggataaa acaggctgtt tcaagttttc aacctgaaaa taatagcgtt attttaggag
     6541 ataatacagt cgtttattat gattttttag tagtagctcc aggattacag attaattggt
     6601 cttcaattaa aggactaaaa gaaaatatag gtaaaaatgg tgtttgctct aactattcac
     6661 ctgactatgt taacgaaact tggaaccaaa tttctaattt taaacaagga aatgccattt
     6721 ttacgcatcc aaacactcct ataaagtgtg gaggtgcgcc tatgaaaatt atgtatttag
     6781 ctgaagatta ttttaggaaa cataaaatcc gttctaacgc taatgtgata tatgcaacgc
     6841 caaaagatgc tttatttgac gtaggaaaat ataataaaga attagaaagg attgttgaag
     6901 aaagaaatat aacagtcaat tataattata accttgttga aatcgacggt gacaaaaaag
     6961 tggctacatt cgaacatatc aaagcatacg atagaaaaac aataagttat gatatgttac
     7021 atgtaacacc acctatgggt cccttagatg tagtaaaaga aagtacactt tcagatagtg
     7081 agggttgggt agatgttaac ccaaccacat tacagcataa aagctactct aatgtatttg
     7141 cacttggtga tgcttcaaat gtacctactt caaaaacagg cgcagctatt cgtaagcaag
     7201 cacctatcgt cgctaataat ttattgcaag tgatgaataa tcaaatgtta acgcatcatt
     7261 atgatggtta tacttcatgc cctattgtta ctggatataa taggttaata cttgcagagt
     7321 ttgattataa taaaaatact aaagaaacaa tgccgtttaa tcaggccaaa gaacgtagaa
     7381 gtatgtatat atttaagaaa gatttattac ctaaaatgta ttggtacggc atgctaaaag
     7441 gattaatata ataaagtaca gaaaacaata aatttttaat gaaaaatctt ttactataaa
     7501 agattaagta tttaaatgac gtgtcagtgt tgtgtttata tgtcgtgaat ttttagctct
     7561 aaatagtata agattgaaaa agttgttact gttttaaatg atcacgatga agtcattcaa
     7621 taagaatgat tatgaaaata gaaacagcag taagatattt tctaattgaa aatcatctca
     7681 ctgctgtttt ttaaaggttt atacctcatc ctctaaatta tttaaaaata attaatggta
     7741 tttgagcacg tttagcgact ttatgactga cattaccaat ttccatttct tgccagatat
     7801 tcaaaccacg tgtactcaaa atgatagctt ggtatgtacc tccaatagta atttcaataa
     7861 ctttgtctgt tgaacactaa gagcaatttt aatttcataa tgtgttgtaa acattttttt
     7921 gattggagtt tttttctgag ttaaacgata tcctgatgta tttttaattt tgcaccattt
     7981 ccaaaaggat aagtgacata agtaaaaagg catcatcggg agttatccta tcaggaaaac
     8041 caagataata cctaagtaga aagtgttcaa tccgtgttaa attgggaaat atcatccata
     8101 aactttatta ctcatactat aattctattt taacgtcttc gtccatttgg gcttcaaatt
     8161 catctagaag tgctcgtgct tctgcaattg attgtgtgtt catcaattga tggcgaagtt
     8221 cgctagcgcc tcttatgcca cgcacataga ttttaaagaa tctacgcaag ctcttgaatt
     8281 gtcgtatttc atctttttca tatttgttaa acaatgataa atgcaatctc aatagatcta
     8341 atagttcctt gcttgtgtgt tcgcgtggtt ctttttcaaa agcgaatgga ttatggaaaa
     8401 tgcctctacc aatcatgaca ccatcgatgc catatttttc tgccagttca agtcctgttt
     8461 ttctatcggg aatatcaccg ttaattgtta acaatgtatt tggtgcaatt tcgtcacgta
     8521 aatttttaat agcttcgatt aattcccaat gtgcatctac tttactcatt tctttacgtg
     8581 tacgaagatg aatagataaa ttggcaatat cttgttcgaa gacgtgcttc aaccaatctt
     8641 tccattcatc gatttcatag tagccaaggc gtgttttaac acttaccgga agcccacctg
     8701 ctttagttgc ttgaataatt tcggcagcga catccggtct taagattaag ccggaaccct
     8761 tacccttttt agcaacattt gctacaggac atcccatatt taagtcaatg cctttaaagc
     8821 ccattttagc tagttgaata ctcgtttcac ggaactgttc tggcttatct ccccatatat
     8881 gagcgaccat cgggtgttca tcttcgctaa aagttaagcg tccgcgcaca ctatgtatgc
     8941 cttcagggtg gcaaaagctt tcagtatttg taaattcagt gaaaaacaca tccggtctag
     9001 ctgcttcact tacaacgtgt cgaaagacga tatctgtaac gtcttccatt ggcgccaaaa
     9061 taaaaaatgg acgtggtaat tcactccaaa aattttcttt cataatatat ttataccctc
     9121 tttataatta gtatctcgat tttttatgca tgatgatatt accacaaaag cctaacttat
     9181 acaaaaggaa tttcaatagt tgcaaccatt tgaaagggaa gtctaagagt agtctaaaat
     9241 aaatgttgtg gtaagttgat caatacaaag atcaaggatt atagtattaa attgttcatt
     9301 attaatgata cactacttat gaatatgatt cagaattttc tttggctact tttacagtaa
     9361 agcgattttt tagttatctt ataacaaaga caaatttata aaggtgatat tatggaagat
     9421 ttaaaggaat ctttaaaaag tttaggttgg tgggatttat tttttgcgat acctatgttt
     9481 ctgctattcg cataccttcc aaactataat tttataacga tatttcttaa cattgttatc
     9541 attattttct tttccatagg tttgatttta actacgcata taattataga taaaattaag
     9601 agcaacacga aatgaatcat taatacgaat gtgattaaac ataaaactga aggagcgatt
     9661 acaatggcga ctaagaaaga tgtacatgat ttatttttaa atcatgtgaa ttcaaacgcg
     9721 gttaagacaa gaaagatgat gggagaatat attatttatt atgatagtgt ggttataggt
     9781 ggtttgtatg ataatagatt attggttaag gcgactaaaa gtgtacgtca tcaattgcaa
     9841 gataatacat tagtttcgcc atatccaggt gccaaagaaa tgatattaat tccagacatt
     9901 accgaagcaa taaatctcac taatttattt gagctcataa aaaatgattt gaaaaactaa
     9961 tcataaattg ccatactcaa ctattttgaa aagtatcaat ttcatattat caaattattg
    10021 agtatctcga tttattgtta tacacaaaca gtatttttgc tatgatagag ttaataaata
    10081 taataaattg aataagtaac tttacaaaac tagaatgatt caattcaatc acaattattt
    10141 gaaaatagag gtgagcaggt gaacgatatg ttaattagtc tcgtaatccc agtttttgct
    10201 ttgttagtta ttggtggtat tatttgggtg attatagaag gtatagtaca tatttcaaaa
    10261 aagaataaag caatcgataa ctttttaaac caagttaata aagtaagtga gacatataaa
    10321 ttcgctactg cttttttatt tctaatcttg gctacggctg gtatttctca attttatcta
    10381 tattatatag tatcagcgtt tcttttttgg cttgtcatac ttacctttgg tattgcaggt
    10441 atcatttttt taatgccata tggattatgt ttcctaccac tttataagca aaaaaagaaa
    10501 aaacagacat ttaaaaaata catggtttac actacgattg gtttgtcaat ttgtctaggc
    10561 ttatctctag ttttggttca cactacgaaa atttatatgg acgaaggtgg cgtaagatac
    10621 tattacggta gttttgtaat gaaacaagcg ggcggttatg cttatttagc tttagcggta
    10681 ctttcaacgt tgttaattgt tgcgaaaaaa gctacaaata aaaataaaga aatcgaaacc
    10741 gtcgacaata caaatataac ggaaagataa ttaagggagt gctcattcag gagtgctctt
    10801 ttttgatgtc caaatttagt tgcaaatgaa ggcatagaag aaatttttat agtcatttat
    10861 tggataataa attatggtca tatcgattaa ttttattagt gaggatttta caaacatttg
    10921 ttgaataaat atggtaatga taatatatga atatttttga aaaaacacta atatgattaa
    10981 attatttgtt aaaatagcat taagtaaaaa acaaataggg aacaaggtgg gatttcatga
    11041 gtcaattgct aaatgatacg ttatcggctt ggttgttaat tgaatcttta agtccaggag
    11101 aagtaaattt tacagcggaa gatatactct cagctgaaca ttttaaaaat ggtgcaaagc
    11161 aagcacaact tcaaagtttt gatgaatatt ttgaaatatg gaattctgaa cgctttatta
    11221 tatcagaaga aaaatcagag actggggaac ttatatttaa attttataga cattgcttcc
    11281 gctataatga aattaatttg aaaattcagg atatttttga tgattattct gatattcata
    11341 atccaaatgg gacacactgt tatggttaca catttaacac agataaacac ggcaaagtga
    11401 tagttgattc tatacatatt ccgatgatta tgagtgcatt aaaagaaatt gaaaagaaca
    11461 aaaatgccaa tatagaagaa aaatttaatg attctgttga aaaatttttt caaaaagtaa
    11521 aagaaatttt agcagatgaa ccaattaatg aatttaaatt gaagaagatg gacaaagctt
    11581 atgatgagta cttttctgta ttaaattcaa agaaagatgg attatttgga cattatgtag
    11641 caatagaata tgtgaaagat agtgatttac cacagccgga atttaacagt ttcttcataa
    11701 gtgatattga gaaagcaaga aaatctccca accaaacttt aattgattac attgaaggtg
    11761 tagaagaaag tcagcgcata gaagtagatg aaaataaaga aatgtttgac aaatttttac
    11821 atccttcacg tttgcctgat ggacgatggc catcacagac tgagtttaga ttgtctttaa
    11881 tgcaacaact tgctgtaaac caaattacga gtggtaatga aagaataagt tcagttaatg
    11941 ggccaccagg gacaggtaag actactttat taaaagatat atttgctcat ctagtagttg
    12001 aaagaggtaa agagttagct aaactaaata atcctaaaga tgcatttgtc aaaacaaaaa
    12061 ttcatgaaac ggatgataaa tacgtatact tactaaagga atctattgcc aaatataaga
    12121 tggtagtcgc atctagtaat aatggagctg ttgaaaatat atctaaagat ttaccgaaaa
    12181 ttgaagaaat tataagaaat cccgaaaaat gtaaattccc taaatatgaa cagaattatg
    12241 caaatttagc acatgaatta aaagattttg ctgaaatagc tgaagatttg attggtgaaa
    12301 gtgcctgggg cttattttct ggagtttttg gtaaaagtac taatattaac caagtattga
    12361 gtcatatgtt aaaacaagat gcgaatgata ttggctttgc taaattacta caaaatgaga
    12421 ataatcgtat gagttataac gagttaatga gtgaatggca atcacatcaa cgtgcatttt
    12481 tagaagagtt gaggcatgtt gaaatgttaa aagaagaatc tattagagca tatgatgttt
    12541 ataaaaattg tgagtctttc tctaagattg aacaggttat taatagtgaa aaaacaagta
    12601 ttgaagaaca ggtatatcat ttagataatg aaacgttacg agacaataaa gaaatagaag
    12661 atttggataa tcgaattaat tatattgtta agcaaataga aactttaaat gagttaatta
    12721 aatccatcaa agaaagcaac aaaggtttta ttaacaaact gaaagcgatg tttaattcag
    12781 aagaagatga aagctataaa gatcataata aagagaagca acaattatta acacaacagt
    12841 tagagttaga gaaatgtaaa aaaaacaaac atgaagacct tgttagcaaa ctaaaagaaa
    12901 aagagaaatt aattaaacaa ttaactaaag tacagttgca attagacgag ttaaattcac
    12961 agttacaaga gttagaagca tatcgtattg agtcaaaaat tacaattcca gaaaaagatt
    13021 tttggagtga caacaattat gatgagcgcc aagttactaa tctgtggacg agtgacgaac
    13081 ttcaatacag acgtgccatg ctctttttaa gagcaatgat attgcataaa ttattattga
    13141 ttgctaataa tacaactatt tattatgcga ttaatgattt taaagataga aggaaattaa
    13201 ttgatgcaaa tccagataaa gtacacaacg catggaatgt gatgcattta atatttccag
    13261 tagttagtac gacgtttgca agctttaaat ctatgtatgg gggcatacca aaagatttca
    13321 tagactactt atttattgat gaagcaggac aagcaatacc tcaagcagct gtgggagcat
    13381 tatatcgttc aaaaaaagtt gtagctgtag gtgatccgat tcaaatagaa ccggttgtga
    13441 ctttagaaag tcatttaatt gataacattc gtaaaaatta tcatgttccg gaatatctag
    13501 tttctaaaga agcttctgtg cagtctgttg cagacaacgc caatcaatat ggtttttgga
    13561 aatctgatgc tactgatagt aatcaaaaaa cctggatagg cataccttta tgggtgcaca
    13621 gacgatgttt aaaacctatg ttcacgatag ctaaccaaat cgcttataat aataaaatgg
    13681 tgttgccaag taatattaca aaagtaggta aaacaggttg gtatgacgtt aaaggaaacg
    13741 cagttcaaaa acaatttgtg aaagagcatg gtgaaaaagt agtgggatta ttagctgatg
    13801 attggattga agcaattaag gaaggtaaaa atgaaccgag ctcatttgta atatcgcctt
    13861 tttcagcagt acagcaacag attaaacgta tgttaaagca acaactaccg actagaattg
    13921 atattgaacg tacaaaaatt aatcaatggg tcgataaatc cattggtact gttcatactt
    13981 ttcaaggtaa agaggctcag aaggtgtatt ttgtaatagg tactgataat acccaagatg
    14041 gtgctgtgaa ctggtcatgc gaaaaaccaa acttgttaaa cgttgcagtg acaagagcta
    14101 agaaagagtt ttatgtaatt ggcgacatgc aaagaataca gatgaaacca ttttatgaga
    14161 cgatttttaa agaaagaaat gtaaaataac atacaaaaaa gtatatagag gaagttatac
    14221 attttaaaag gagcaaaatt gaataatgga gaaatttaac aactggatat taaatgcaat
    14281 aagtggatct caaacagaca agaatgaaac aactgaagaa ttaaaagggg caaaatttat
    14341 cattttatat gcatattcaa tgctcgtttt gcttgcgtta gtaatttcta acatattcat
    14401 tcacattttg gagcctaaac tatcaatcac cactcaaatc atcatcgttt tgattttaat
    14461 tgaagcacta attggactgc gtttcttgaa agcgtacgat gttaagcgtg gcaaagataa
    14521 agaaaataag aaaaatagta aggatttcgt taaactaaaa tcaattttag tagcaatttt
    14581 atttacatca ttggcgctga cagcaggtac tgtagctgat atatacggtt tcactgactt
    14641 aggaaatact agaagtgatt taatcgtttg gagcataggt ggtattatat ttggcctcgt
    14701 atgttacaca atggaagata aaaaataacg ataaggagct ggcgattata aagctagctc
    14761 cttttttaac ttatatatgt aaagcactat cctaagggtc tttaatcata tgtcaataat
    14821 tcctataatt aatactctta aatattgttt aaataagttt taaaatttta cacatatttt
    14881 tatttaaaaa agatgtataa ttaatgtatt aaatatagaa agaagttgat attatgaaaa
    14941 agtgtattaa gactttgttt ttaagtatca ttttagtagt gatgggtggt tggtatcatt
    15001 cagcacatgc gtcagattcg ttgagtaaaa gtccagaaaa ttggatgagt aaacttgatg
    15061 aaagcaaaca tttaactgag attaatatgc caggctcaca tgatagtggc tcattcactt
    15121 taactgatcc ggtaaaatcg gtttgggcaa agactcaagg caaagattac cttactcaaa
    15181 tgaagtcggg agtcaggttt tttgatatta gaggtagagc aagtgctgat aatatgattt
    15241 cagttcatca cggcatggtt tatttgcatc atgaactagg aaaattttta gatgatgcta
    15301 aatattactt gagtgcttat ccaaacgaaa caattgtgat gtctatgaaa aaggactacg
    15361 atagcgattc taaagttacg aagacatttg aagagatttt tagagaatat tattataata
    15421 acccgcaata tcagaatctt ttttacacag gaagtaatgc gaatcctact ttaaaagaaa
    15481 cgaaaggtaa aattgtccta ttcaatagaa tggggggtac atacataaag agtggttatg
    15541 gtgctgacac gtcaggtatt caatgggcag acaatgcgac atttgaaacg aaaattaata
    15601 atggtagctt aaatttaaaa gtacaagatg agtataaaga ttactatgat aaaaaagttg
    15661 aagctgttaa aaatttattg gctaaagcta aaacggatag taacaaagac aatgtatatg
    15721 tgaatttctt gagtgtagcg tctggaggta gcgcatttaa tagtacttat aactatgcat
    15781 cacatataaa tcctgaaatt gcaaaaacga ttaaagaaaa cgggaaagct agaactggtt
    15841 ggctgattgt tgactatgca ggatatccgt ggcctggata tgatgatatc gtaagtgaaa
    15901 ttatagatag taataaataa ggattcaata atgatattaa gacgagtatg aaaatagtta
    15961 gattctaatt attttcacta ctcgttttta ttttgaaaat aagtaataat tcaacaatat
    16021 tataaattga acagattgtt tgtgaaattt ttgataatat taaagtgaaa aagtgttata
    16081 aattgataaa tatatgtaat taacaaaaac aaatcatttt aaaaagaaga gagttgtaag
    16141 atgatgaaac gattaaacaa attagtgtta ggcattattt ttctgttttt agtcattagt
    16201 atcactgctg gttgtggcat aggtaaagaa gcggaagtta agaaaagctt tgaaaaaaca
    16261 ttgagtatgt accctattaa aaatctagag gatttatacg ataaggaagg ctatcgtgat
    16321 gatcagtttg ataaaaatga taaaggtaca tggattataa attctgaaat ggttattcaa
    16381 cctaataatg aagatatggt agctaaaggc atggttctat atatgaatag aaataccaaa
    16441 acaacaaatg gttactacta tgtcgatgtg actaaggacg aggatgaagg aaaaccgcac
    16501 gacaatgaaa aaagatatcc ggttaaaatg gtcgataata aaatcattcc aacaaaagaa
    16561 attaaagata aaaacataaa aaaagaaatc gaaaacttta agttctttgt tcaatatgga
    16621 aactttaaag atttgtcgaa gtacaaagat ggagatattt catacaatcc agaggtgcca
    16681 agttattcag caaaatatca agtaactaat gatgattata atgtaaaaca attacgtaaa
    16741 agatatgata taccgacgaa taaagcacca aagttattgt tgaaaggtac aggtaattta
    16801 aaaggctcat cagttggata taaagatatt gaatttactt tcgtggagaa aaaaggtgaa
    16861 aatatatact ttagtgatag tctacatctt gaaccaagtg aggataaata aacgtgacca
    16921 ataaagagta tgaaatcgaa cccggaaaaa gagagtgatg aaatgataaa acgtgtaaat
    16981 aaattagtgc taggtattag ttttctgttt ttaatcatca gtatctttgc tggttgtggt
    17041 acagggaaag aagcggaaat aaagaaaagt tttgaaaaaa cgttgagtat gtatccgatt
    17101 aaaaatctag aggatttata cgataaagaa ggatatcgtg atgatcaatt tgataaaaat
    17161 gataagggaa catggattgt tcgttcgtca atgtcgattc aatctaatgg aaaagatatg
    17221 aatataaaag gcatggtttt atatatgaat agaaatacaa gaacaacaaa tggctattac
    17281 tatgttgatg ttatagaaag agaagataaa ggtatacatc gtgataacga aaaaagatat
    17341 ccggttaaaa tggtcgataa taaaatcatt ccaactaaag aaattaaaga tgaaaaaata
    17401 aaaaaagaaa tcgaaaactt taagttcttt gttcaatatg gcgactttaa agatttgtcg
    17461 aagtacaaag acggcgatat atcatacaat ccagaggtgc cgagttattc agcaaaatat
    17521 caattaacta atgatgacta taatgtaaaa caattacgaa aaagatataa tataccaacg
    17581 aataaagcac caaagctatt gttgaaaggt acagggaatt taaaaggttc atcaattggc
    17641 tacaaaaaaa ttgaatttac tttcgtagag aaaaagggag aaaatatata ctttagtgat
    17701 gggctacatt ttaatccaag tgaggataaa taatcatgat tcgatcaaat tatgaaatcg
    17761 atccaggaaa aacaataaaa agaaatagga ttgtgaaatt atgaaacgat taaatacatt
    17821 ggtattgtac atcagttttt tgatattaat catcagtatc gttgctggtt gtggtacagg
    17881 gaaagaagcg gaaataaaga aaagttttga aaaaacattg agtatgtatc caattaaaaa
    17941 tctagaggat ttatacgata aagaaggcta tcgtgatgac gaatttgata aaaatgataa
    18001 aggaacgtgg actattagtt ctgaaatggc aattcagaaa aagggagaag ccttaaatat
    18061 aaaaggtatg gttttgaagt taaatagaaa tacaaggagt gcaaaaggat tttactatgt
    18121 taatgcgata aagaaggatg aaaatggcag acctcaggat aagcaaatag aatatcccgt
    18181 taaaatgatt gacaataaaa tcattccaac taaagatatc aaagatgaaa aaataaaaaa
    18241 agaaatcgaa aactttaagt ttttcgcgca gtatggcaat tttaaagatt tgacgaagta
    18301 taaaggtgga gatatttcat ataatccaga agccccgatt tattcagcaa aatatcaact
    18361 aaccaatgat gattataatg taaagcaatt acgtaaaaga tatgatatac cgacgaataa
    18421 agctccaaag ttattgttaa aaggttcagg aaatttagat ggttcatcaa ttggatacaa
    18481 aaaaattgaa tttactttcg tagagaaaaa aggagaaaac acttacttta cagctaacct
    18541 acattttaaa ccgagtaatg atgaataatc gtgactaata aagagtatga aatcgattca
    18601 aggaaacaaa ataaaaagaa gagagttgta aggtgatgaa acgtttaaat aaattagtgt
    18661 taggtattag tttcttattt ttagtcatta gtattactgc tggttgtggc atgggtaaag
    18721 aagcggaaat taagaaaagt tttgaaaaga caatgagtat gtatccgatt aaaaacctag
    18781 aggatttata tgacaaagag ggctatcgag atgatcaatt tgataaaaat gataagggaa
    18841 catggattgt aaattctcaa atggcaattc aaaataaagg agaacctatg aaatcaaaag
    18901 gcatggttct gtatatgaat agaaatacaa gaacaacaaa tggctattat tatgttaatg
    18961 tgattaaggg cgaggataaa ggtaaacatc aagacaatga aaaaagatac cctgttaaaa
    19021 tggtcgacaa taaaataatc cttactaaag aaattaaaga tgaaaacata aaaatagaaa
    19081 tcgaaaactt taagtttttt gttcaatatg gaaactttaa agatttggaa aattataaag
    19141 atggagatat atcatataat ccagaggtgc caagttattc agctaaatat caattaacta
    19201 atgatgatta caatgtaaag caattacgta aaagatatga cataccaaca aataaagcac
    19261 cgaagttgtt gttgaagggt acagggaatt taaaaggttc atcagttgga tataaagaca
    19321 ttgaatttac gtttgtagag aaaaaagaag agaacattta ctttagtgat gggttaattt
    19381 ttaaaccaag tgaggataaa taatcatgac tcgattaaaa tatgaaatca atccaggaaa
    19441 gataataata agaataggat tgtgaaatta tgaaacgatt aaacaaattg gtattgtata
    19501 ttagtttttt gatattagtc attagtttca ctgctggttg tggcatgggt aaagaagcgg
    19561 aaataaagaa aagttttgaa aaaacattga gtatgtatcc gattaaaaat ctagaggatt
    19621 tatacgataa agaaggctat cgtgatgatc aatttgataa aaacgataaa ggcacatgga
    19681 ttattaattc tgaaatggtt gttcaaccta aaggggaacg tatgaaatca aaaggtatgg
    19741 ttctatatat gaatagaaac actaagacta caaccgggaa gtatatagtg agtgaaacac
    19801 ttcatgatga agatggtaga cctaagagta aagataaaga atatccggtt aaaatggtag
    19861 acaataaaat cattccaact aaaggtatca aagacgaaaa tataaaaaaa gaaatcgaga
    19921 attttaagtt cttcgcgcaa tatggcagct ttaaagattt gtcgaagtac aaagacggcg
    19981 atatatcata taatccagag gtaccgagtt attcagcaaa atatcaatta actaatgatg
    20041 actataatgt aaaacaatta cgaaaaagat ataaaatacc aacgaataaa gcaccaaagt
    20101 tattgttgaa aggttcggga gacttaaaag gttcatcagt tggatataaa gacattgaat
    20161 ttacttttgt ggagaaaaaa ggtgaaaata ctttttttac tgatagtcta catcttgaac
    20221 caagtgagga taaataatct cctgcctccc acacgattca acacgaattt caaatatgtt
    20281 gctgtaaaat aatgtaaaat aaacgtattc atattactga attgagggat agtatgcaac
    20341 gtgattattt aattcgagta gaaactgaaa gcatgccaga tttcaaaagg ctcaatggtt
    20401 taatgattgg ttttgttatt aaaggtgagg cacatattta tgatgaaaat aacatgacgc
    20461 aatgcaacag tggcgacatt ttcatcatta accaccgtga cttgtatcga tttcaacttc
    20521 aacaagatgg catcatatgt tatatccaat tccaaatgaa atatttggca gacaagtttg
    20581 atgatgcgca ttgtctatat tttcacttaa cagatgcgac cacaaccaag aatatacatc
    20641 aactgagaaa tataatggca agactggttt caacacacat tcgacataat gagttgtcta
    20701 aattgactga gcaacaactt gtgattcagt tgcttatgca tatgattcat tatgtcccgc
    20761 gtacatatca ttcgaaccaa agtatcttaa atgatgataa agtgaatcaa gtatgcgact
    20821 atatcgagtt acattttcat gaagatttaa gcctttcaga attaagcgaa tacgttgggt
    20881 ggtcagagag ccatctgtct aaaaagttta cagaatcgct aggtgtagga ttccaacatt
    20941 tcttaaatac gacgcgaatt gagcatgcga aactcgattt aacatacaca gatgaaacga
    21001 ttactgatat tgcattgcaa aatggctttt caagtgcagc gagctttgcg agaacattta
    21061 aacactttac gcatcaaacg cctaaacaat atcgaggtga tcgtccagca atcactgaaa
    21121 atcaacaatc ggcacaacat aattatcacg accgtgaatt gatattactt ttaaatgact
    21181 acattgaaga aatgaatcat ttcattgaag atattgaaaa gatgaactat aaagagattg
    21241 cctttaaacc aactaatcaa caactaaatc aatttaatca tattattcaa gtgggctatt
    21301 tgaggaattt gctcaataca cagtatcaat cacagttgct tacatgtcat catgattttc
    21361 aagtcaatga agtattagca tatgatgtga tgccatatat tatgaaaaag ctcaatgcgc
    21421 cattcacgta tgatgcagag atttcgaata tattttatga tatcgatttg tgtttagact
    21481 ttttattaga tcataacttt agtctaacca tgcatttgaa tcagtatgac tcacgagatt
    21541 atatcgatgc attcaaagta tttatccatc acgttgccct gcatgtcagt catagaaaag
    21601 atttgaagtt caacttgtat gtgacgacat tgcacacgtc tttaattgaa atgattgatt
    21661 attttaaagc attattccct aatggtggct tgtacattca cttagatcaa gctacggaaa
    21721 gacatctacc attgttgaaa cgacttgagc cacacatcaa ccattttgta tttgatgcca
    21781 attcaaatga tgctgttgat tttaataaaa tgaatgatga tgaatttaaa accgcgagtc
    21841 aaatgattat taataaaacg aattacctta tcgacttaat gcatcgccat aacctaaagc
    21901 gtccactcat tttactcaat tggaatacat tgacgggtga tacatttatt acaaacggcg
    21961 aatattttag aggtggtatc atcattgagc aattattaaa attaagtact aaagtagagg
    22021 gtatcgggta ttggttgaat tatgatttgc acgtcagtca ttgtagaaat gaacgggatt
    22081 atatgaattc tattgaactg tttcatcaat ataatggaaa acgtccggtc tatttcacgg
    22141 cattgctatt taataaatta acaagcaata ttttgtattc tgatgataca tgtattgtca
    22201 cgggaactga ttcaaatttt caaatattgt tatatgatgc aaagcatttt aatccgtact
    22261 tagcgttgga caatcaaatg aatatgcgtg caacggaaat gatccatttg aacattaatg
    22321 ccctagaaga cggtatgtat aagattaaac attttacctt agataaagaa aatggtgcgt
    22381 tatttaatct ttggcgcaaa catcatacga tacatggcat ggacaaggac tctatagatt
    22441 acgttaatcg aatgagtttt ccgaaattag aagtatatga tatagatatc acggacacac
    22501 tggcattaaa cattaaaatg attacgaatg ggattcactt aattgaagta aaacgttacc
    22561 caagttcata aaatgatcac aaatcacaaa ttttgatata cataatttgt gattttttat
    22621 attcaaagct aaaattgcaa aaaattaatg gttaacatct ctgttgttgg caatataaat
    22681 aatgattaat catttatgat gtaactaagg agatgaagga tatgaatcaa caattaattg
    22741 aagctttaaa atctaaagaa gacaaaatga ttgagatcag acgttattta catcagcatc
    22801 cagaattatc ttttcatgaa gatgaaacgg cgaaatacat cgctgaattt tacaaaggta
    22861 aagatgtgga agtagaaacg aatgtcggac cacgtggaat taaagtaacg attgattcag
    22921 ggaaacctgg taaaacatta gcaatccgtg cagactttga cgcattaccc attactgaag
    22981 atacaggatt atcttttgca tcacaaaata aaggtgttat gcacgcatgt ggtcacgatg
    23041 cacatacagc ttacatgctt gtattagcag agacgcttgc tgaaatgaaa gatagtttta
    23101 caggaaaagt cgttgtgata catcaaccag ctgaagaagt accaccaggt ggtgctaaag
    23161 caatgattga aaatggtgta ttagacggtg ttgatcatgt attaggtgta cacgtcatga
    23221 gcacaatgaa aacaggtaat gtgtattaca gacctggtta tgttcaaaca ggacgcgcat
    23281 tcttcaaatt gaaagttcaa ggtaaaggtg gtcatggttc atcaccacat atggccaatg
    23341 atgccattgt tgcaggtagc tacttcgtca cagcgttaca aacagttgta tctagacgac
    23401 taagtccatt tgaaaccggt gttgtcacaa tcggttcatt tgacggtaaa ggtcaattca
    23461 atgtcattaa agatgttgtt gaaattgaag gtgatgtacg tggattaaca gatgctacaa
    23521 aagcaacaat tgaaaaagaa attaaacgtt tatcaaaagg attagaggat atgtatggtg
    23581 taacttgcac cttagaatat aacgatgatt atccagcatt atataatgat ccagagttta
    23641 ctgagtacgt ggctaagacg ttgaaagaag caaaccttga ttttggtgtt gaaatgtgtg
    23701 aaccacaacc accttcagaa gactttgcat attatgctaa agaacgtcca agtgccttta
    23761 tttatacagg tgcagctgtg gaagatggtg aaatttaccc acatcatcat cctaaattta
    23821 acatttcaga aaaatcatta cttatttcgg cagaagctgt agggacagtt gttttagatt
    23881 accttaaagg agataactaa catgaatgaa acgtatcgcg ggggcaacaa gttaatctta
    23941 ggtattgtat taggtgttat tacattttgg ttgtttgcac aatcacttgt aaatgttgta
    24001 ccaaatttac aacaaagttt tggtgcagac atggggacaa ttagtatagc ggtcagtcta
    24061 actgcactat tttcaggtat gtttgtcgtt ggagcaggtg gtctagcaga taaaattggg
    24121 cgcgtgaaaa tgacgaatat cggtttattg ttaagtatta ttggttcagc attaattatt
    24181 attacgaatt taccggcatt attaatttta ggtcgtgtta tacaaggtgt atcagcagcg
    24241 tgtattatgc cttccacatt ggccattatg aaaacttatt atcagggtgc tgaacgtcag
    24301 cgtgccttaa gttattggtc tatcggttct tggggtggca gtggtatctg ttcactcttc
    24361 ggtggtgcag ttgcgacaac tatgggttgg agatggattt tcatcttctc aattatcgtt
    24421 gccgtacttt caatgttact catcaaaggg acgcctgaaa cgaaatcaga aattaccaat
    24481 acacataaat ttgacgttgc agggctaatt gttctagtag ttatgttgct aagtttaaac
    24541 gttgtcatta ctaaaggtgc agcacttggt tacacatcat tatggttctt tggtttgatt
    24601 gcaatcgtaa ttgtagcatt ctttattttc ttaaatgttg agaaaaaagt agataatcca
    24661 cttattgatt ttaaattatt tgaaaataaa ccatatacag gtgcaacgat ttcgaacttc
    24721 ttattaaacg gtgttgcagg tacattaatt gtagcgaata cattcgtgca acaaggttta
    24781 ggttatacag cattgcaggc aggatactta tcaattactt atttaatcat ggtgttattg
    24841 atgattcgag ttggtgaaaa attattacaa aaaatgggtt ctaagcgacc aatgttatta
    24901 ggtacattca ttgtggtcat tggtattgca cttatttcat tagtattctt accaggcata
    24961 ttttatgtta tcagttgtgt cgtaggatat ttatgtttcg gactaggctt aggtatttat
    25021 gcaacacctt ctacagatac agctatttcg aatgcaccgt tagataaagt tggcgttgct
    25081 tcaggtattt ataaaatggc ttcatcactt ggtggcgcat tcggtgtcgc aattagtggt
    25141 gctgtatatg ttggtgcagt tgctgcaacg agcattcata caggtgcgat gattgcactt
    25201 tgggttaacg tattaatggg aatcatggca tttatcgcaa ttttattcgc gattcctaat
    25261 gatgataaac gtgtcaaaga tgcgaaataa taacgatgac acgccaagtg ttttgataat
    25321 gatataaatg aaagtaaagc aaattaaatg aggaaatgta tagagcagtg catatagata
    25381 aatctatgat tggattaaca ctaatataaa
//
LOCUS       DAOBBH010000019        25262 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.019, whole genome shotgun sequence.
ACCESSION   DAOBBH010000019 DAOBBH010000000
VERSION     DAOBBH010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25262
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.019"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            192..449
                     /locus_tag="P6L01_000540"
     CDS             192..449
                     /locus_tag="P6L01_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDP3029631.1"
                     /translation="MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLE
                     DWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDIDWDNL"
     gene            449..715
                     /locus_tag="P6L01_000541"
     CDS             449..715
                     /locus_tag="P6L01_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDP3029632.1"
                     /translation="MSNYTVKIKNSAKSDLRKIKHSYLKKSFLEIVETLKNDPYKITQ
                     SFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLILSAWSHYD"
     gene            1070..2146
                     /locus_tag="P6L01_000542"
     CDS             1070..2146
                     /locus_tag="P6L01_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDP3029633.1"
                     /translation="MNQSVKLLKHLTDVNGIAGYEMQVKEAMRNYIEPVSDQIIEDNL
                     GGIFGKKNAENGQYSIMISGHMDEVGFMVTKIDKHGFISFTPVGGWWNQVMLSQKVTI
                     TTDSGKEIRGIIGSKPPHVLTPEERKKPMEIKNMFIDIGVSSKEEAEEAGVEVGNMVT
                     PYSEFEVLANDKYLTAKAFDNRYGCALAVEVLKRLKDENIGINLYSGATVQEEVGLRG
                     AKVAANTIKPDLAIAVDVGIAYDTPGMSGQTSDSKLGGGPVVIMMDATSIAHQGLRKH
                     IKDVAKEHNIEVQWDTTPGGGTDAGSIHVANEGIPTMTIGVTLRYMHSNVSVLNVDDY
                     ENSIRLVTEIVRSLNDESYKNIMW"
     gene            2394..3056
                     /locus_tag="P6L01_000543"
     CDS             2394..3056
                     /locus_tag="P6L01_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDP3029634.1"
                     /translation="MLLEIKDLVYKASDRIILDHISLKVDKGESIAIIGPSGSGKSTF
                     QKQICNLISPTSGELYFKGKPYNDYDPEELRQRISYLMQQSDLFGETIEDNMIFPSLA
                     RNDKFDRKRAKQLIKDVGLGHYQLSSEVENMSGGERQRIAIARQLMYTPDILLLDEST
                     SALDVNNKEKIENIIFKLVEQDVAIMWITHSDDQSMRHFQKRITIVDGKISKVEELNQ
                     HE"
     gene            3049..3825
                     /gene="fetB"
                     /locus_tag="P6L01_000544"
     CDS             3049..3825
                     /gene="fetB"
                     /locus_tag="P6L01_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDP3029635.1"
                     /translation="MSNTALGLTALLLVIPIIISYKEGLHIIKDLIVATLRAVVQLII
                     LGFLLHYIFKINDKWLLILCVLVIIINASWNTISRASPVMHHVFWISFLAIFIGTALP
                     LAGTVATGAIHFTANEVIPIGGMLANNGLIAINLAYQNLDRAFVQDGTNIESKLSLAA
                     TPKLASKGAIRESIRLAIVPTIDSVKTYGLVSIPGMMTGLIIGGVPPLQAIKFQLLVV
                     FIHTTATIMSALIATYLSYGQFFNARHELVARNTDVKSES"
     gene            4221..5408
                     /locus_tag="P6L01_000545"
     CDS             4221..5408
                     /locus_tag="P6L01_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDP3029636.1"
                     /translation="MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQL
                     VTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIIS
                     SAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYT
                     FLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHEAPSHENHVTSKILRPAEVAKYLIIT
                     FLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRW
                     LMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQV
                     MSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTF
                     KSS"
     gene            complement(5470..6822)
                     /locus_tag="P6L01_000546"
     CDS             complement(5470..6822)
                     /locus_tag="P6L01_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HDP3029637.1"
                     /translation="MKINTTGGQIHGITQDGLDIFLGIPYAEPPVHDNRFKHSTLKTQ
                     WSEPIDATEIQPIPPQPDNKLEDFFSSQSTTFTEHEDCLYLNIWKQHNDQTKKPVIIY
                     FYGGSFENGHGTAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKDFHSNNGLS
                     DQINVIKWVHQFIESFGGDANNITLMGQSAGSMSILTLLKIPDIEPYFHKVVLLSGAL
                     RLDTLKSARNKAQYFQKMMRDYLDTDDVTSLSTDDILMLMAKLKQSRGPSKGLDLIYA
                     PIKTDYIQNDYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDV
                     QTAKQQSLAITHCYFKQPMKQFLQHLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILD
                     MVFWFGNLQILAAHQYPTTAHLKFLSRQMQNDLANFAKSGKMPWPMYHNERRYYRTYQ
                     "
     gene            complement(7131..8768)
                     /locus_tag="P6L01_000547"
     CDS             complement(7131..8768)
                     /locus_tag="P6L01_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDP3029638.1"
                     /translation="MNKDKQLHNDKINLSQLVLLGLGSLIGSGWLFGAWEASSIAGPA
                     AIISWVLGFLVIGTIAYNYIEIGTMFPQSGGMSNYAQYTHGSLLGFIAAWANWVSLVT
                     IIPIEAVSAVQYMSSWPWHWAKPMRYLMENGSISTYGLLAVYLIIVIFSLLNYWSVKL
                     LTSFTSLISVFKLGVPMLTIIMLMLSGFDTSNYGHSASTFMPYGSAPIFAATTASGII
                     FSFNSFQTIINMGSEIKNPEKNIARGIAISLSISAVLYIILQSTFITSMPQSMLQHSG
                     WNGINFNSPFADLAILLGINWLAILLYIEAFVSPFGTGVSFVAVTGRVLRAMEKNGHI
                     PKFLGKMNEKYHIPRVAIIFNAIISMIMVTLFRDWGTLAAVISTATLVAYLTGPTTVI
                     ALRKMGPTMTRPFRAKILKVMAPLSFVLASLAIYWAMWPTTAEVILIIILGLPIYFFY
                     EYRMNWRNTKKQIGGSLWIIVYLIVLSILSFIGSKEFKGLNMIHYPFDFIVIIVVALI
                     FYYIGTTSSFESVYFRRATRINTKMRESLNNESKSSH"
     gene            8948..9547
                     /locus_tag="P6L01_000548"
     CDS             8948..9547
                     /locus_tag="P6L01_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family protein"
                     /protein_id="HDP3029639.1"
                     /translation="MSEKQSNEQVEAFLNKESQWQEEYKYLRALIFNETELEEAYKWM
                     HPCYTLNNKNVVLIHGFKNYVALLFHKGAILEDKYHTLIQQTEKVQAARQLRFENLTE
                     IQARTEEIKYYLAEAIKAEKAGKKVEMKKTEEYVIPKELEAKFEEMPQLESSFYKLTP
                     GRQHQYIYHIGQAKRSETRQKRVEKYINQILEGKGMHDK"
     gene            9551..9691
                     /locus_tag="P6L01_000549"
     CDS             9551..9691
                     /locus_tag="P6L01_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029640.1"
                     /translation="MSKAYRLYNNIQDDTKQPMAHAVGCFFRCVCHGQHFDVGIPLQA
                     WE"
     gene            9771..9890
                     /locus_tag="P6L01_000550"
     CDS             9771..9890
                     /locus_tag="P6L01_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029641.1"
                     /translation="MGGCPSVTMDACALLQKFDFCNNISHFRHFFAIKQPIER"
     gene            10217..11443
                     /locus_tag="P6L01_000551"
     CDS             10217..11443
                     /locus_tag="P6L01_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3029642.1"
                     /translation="MLSIKHLTKIYSGNKKAVDDISLDIQSGEFIAFIGTSGSGKTTV
                     LRMINRMIEATEGQIEIDGKDVRSMNPVELRRNIGYVIQQIGLMPHMTIKENIVLVPK
                     LLKWTKEEKDKRAKELIKLVDLPESFLERYPAELSGGQQQRIGVVRALAAEQDIILMD
                     EPFGALDPITRDTLQDLVKTLQRKLGKTFIFVTHDMDEAIKLADKICIMSEGKVVQFD
                     TPDNILRHPANDFVRDFIGQNRLIQDRPNDKTVEGVMIKPITIQADATLNDAVHIMRQ
                     KRVDTIFVVDSNNHLLGFLDIEDINQGIRGHKSLRDTMQQHIYTVQIDSKLQDSVRTI
                     LKRNVRNVPVVDDKQRLVGLITRANVVDIVYDTIWGDSEDAVQTEHVGEDTVSSKMHE
                     QHTTNVKVRDIGDDKS"
     gene            11440..12075
                     /locus_tag="P6L01_000552"
     CDS             11440..12075
                     /locus_tag="P6L01_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3029643.1"
                     /translation="MIEFLHEHGGQLMSKTLEHFYISIVALLLAIIVAVPIGILLSKT
                     KRTANIVLTVAGVLQTIPTLAVLAIMIPIFGVGKTPAIVALFIYVLLPILNNTVLGVQ
                     NIDSNIKEAGKSMGMTQFQLMKDVELPLALPLIIGGIRLSSVYVISWATLASYVGAGG
                     LGDFIFNGLNLYDPLMIVTATVLVTALALGVDALLALVEKWVVPKGLKVSG"
     gene            12092..13033
                     /locus_tag="P6L01_000553"
     CDS             12092..13033
                     /locus_tag="P6L01_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDP3029644.1"
                     /translation="MKKIKYILVVFVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSE
                     SQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGA
                     LKEAPIKDPKKAMIATQQGFKKKFDQTFFDSYGFANTYAFMVTKETAKKYHLETVSDL
                     AKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNTEKLDVAL
                     GYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSE
                     MQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK"
     gene            13033..13728
                     /locus_tag="P6L01_000554"
     CDS             13033..13728
                     /locus_tag="P6L01_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3029645.1"
                     /translation="MEGNLLQQLFNYYVTNFGYLWDLFFKHLLMSVYGVLFAALIGIP
                     LGILLARYTKLSGFVITIANIIQTVPVIAMLAILMLVMGLGSETVVLTVFLYALLPII
                     KNTYTGIASVDANIKDAGKGMGMTRNQVLRMIELPLSVSVIIGGIRIALVVAIGVVAV
                     GSFIGAPTLGDIVIRGTNATDGTTFILAGAIPIAIIAIVIDVLLRFLEKRLDPTTRHR
                     KNQSNHRPQSINM"
     gene            14022..15422
                     /locus_tag="P6L01_000555"
     CDS             14022..15422
                     /locus_tag="P6L01_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDP3029646.1"
                     /translation="MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSS
                     DVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVVGSLLIIITPLPAF
                     LIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLMA
                     TYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLS
                     LNVIITQTSHFGLVSPLILGLIVVFICSLVGFVYYENKIKHPLVDFSIFKNRGYSGAT
                     ISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLIAVLSMIRVGEKILSQHG
                     PKRPLLLGSGFTVIGLILLSLTFLPEVWYIISSIVGYLLFGTGLGLYATPSTDTAVAS
                     APDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNALLAIV
                     AFLVILLLVPKNQTNL"
     gene            15648..16583
                     /locus_tag="P6L01_000556"
     CDS             15648..16583
                     /locus_tag="P6L01_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDP3029647.1"
                     /translation="MKIAIAGSGALGSGFGAKLFQAGYDVTLIDGYTSHVEAVKQHGL
                     NITINGEAFELNIPMYHFNDQRDESIYDVVFLFPKSMQLKEVMEDMKPHIDNETIVVC
                     TMNGLKHEEVIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENV
                     IKVADLLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLI
                     YKLTQEIVHVATIDNVHLNVDEVFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYIN
                     GAVATLGKQRHIEAPVNRFITDLIHTKESQRHAQD"
     gene            16905..17756
                     /locus_tag="P6L01_000557"
     CDS             16905..17756
                     /locus_tag="P6L01_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDP3029648.1"
                     /translation="MSKIFVTGATGLIGIKLVQRLKEEGHEVAGFTTSENGQQKLAAI
                     NVKAYIGDILKADTIDQALADFKPEIIINQITDVKNVDMAANTKVRIEGSKNLIDAAK
                     KHDVKKVIAQSIAFMYEPGEGLANEETSLDFNSTGDRKVTVDGVVGLEEETARMDEYV
                     VLRFGWLYGPGTWYGKDGMIYNQFMDGQVTLSDGVTSFVHLDDAVETSIQAIHFENGI
                     YNVADDAPVKGSEFAEWYKEQLGVEPNIDIQPAQPFERGVSNEKFKAQGGTLIYKTWK
                     DGMNPIK"
     gene            complement(17848..19344)
                     /locus_tag="P6L01_000558"
     CDS             complement(17848..19344)
                     /locus_tag="P6L01_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDP3029649.1"
                     /translation="MTTKKLYFLSISIIILVAISIAIYITLNSNTKTRLTNDSQQQID
                     KIIEHDLQKGHIPGASILIVKNGKVFLNKGYGYQDVDKKVKASPTTKYEIASNTKAFT
                     GLAILKLAQEGRLNLNDDVSKHVPHFKMNYNGQNETITIKQLLAQTSGIPSDITSEDA
                     VTNKNNRLNDVTRAIMGDELHHKPGEEFEYSNMNYDLLGLIIQNVTKQSYTKYITNSW
                     LKPLHMTHTSFKQTNNKSKHDAIGYELQGSTPVVSKPEFNLWDTPSAYMMTSTEDLEH
                     WIKFQLNPPDKYKSLVQQSHKNLSSTIGEPNANAYASGWFTNNDEHLVFHSGTLDNFS
                     SFILLNPKQNYGIVVLANLNSEYVPKLVEHLNTQIVNHKRYSTVASILNQYKDQFNIV
                     TVLMTTLILLAFIFSAYRAWQMRHGQILLRRSKRIAVLSWLTLCLCIAIALILYALPY
                     LILGSNNWSFVLTWLPIEIKLALITTLIALFSTLIVILLFLHTKITKT"
     gene            19576..21405
                     /locus_tag="P6L01_000559"
     CDS             19576..21405
                     /locus_tag="P6L01_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDP3029650.1"
                     /translation="MFNQFKRLIIGQPKKNRELKDEKISKFKGLAILSSDALSSVAYG
                     PEQILITLSVVGAVATWYTLPIAGAVLILLAALIMSYRQIIYAYPKGGGAYMVSKTNL
                     GEKWGLLAGGSLLVDYILTVAVSISSGADAFVAAFPSLYGHKVLIACLLVLFILILNL
                     RGLTESATVLSYPVYLFIIGLVILIFIGTFRVATGDIQPHMHASVGTAVPGVTLFLLL
                     KAFSSGASSLTGVEAISNAVTNFREPSANNAVKTLIAMGSILAFLLVGIVGLAYVYGI
                     MPQTETTVLSQLAMQIFGDNAAFYFVQATTVMILVLAANTGFTAFPMLAASMSKDKYM
                     PRMFTVRGDRLGYSNSIIILGVLAIILIIVFDGMTEDLIPLYAVGVFIPFTLAQFGMV
                     IKWIHERPKNWLSKLSVNLLGGIVTFIVFMILLITKFSQVWPILIFLPFVVIFFLKIN
                     KHYRDIAEQLRSDIDVLNVDVVDRNLAIVPITSITTAVDKSIYYAQMLANNDVIAVHV
                     SFGDEDEKAFQEKWKRHFPDVRLVILHSEYRSIIRPISRFIDKINRKANDQNYMITVV
                     IPEFITKKRWHNLLHNQTSLRMKLYLIYQKNVNVCTIPFKLKK"
     gene            complement(21546..23624)
                     /locus_tag="P6L01_000560"
     CDS             complement(21546..23624)
                     /locus_tag="P6L01_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDP3029651.1"
                     /translation="MALLEAFLIFIFAVIISSVINNRFPQIPTAFIQIALGVVIFIIP
                     IQVDFQFNSEVFMFAVIAPLLFVEGTHVSRTKLLEYRKPILLMSMALVFATVVGVGYF
                     IHWIWPALPMPAAFAIAAILCPTDAVAVSAITRGKLLPKGSMTILEGESLLNDAAGII
                     SFKIAVTALVTGTFSLFQAVEQFIISTILGVLIGAIIGFVVVRIRIDLTANKGLKDNN
                     TLTFIQLLTPFVVYFLAEEVHASGIIAVVIAGLIHGLERDRLIRAQTELQMNYHQIWN
                     TFSYALNGFVFVVLGFMIPTVVIDIFQTEPDNFSFLIVITILIAIAIYACRFVWVYFW
                     YKDFYFPKNIQSYLDEEHDSHETPPSRVRYAFIMTMCGIHGTISLSMALTLPFIITKG
                     QAFEYRNDLLFIASFMVLISLILAQIVLPLITPSAEDTTFKGMTYQSAKIFIVQKVIQ
                     HFKNESKKDKNDTNYRPVLNQYYGELLFLLNSEPNNQNTKELKRLEDIAKVIETSTLE
                     RLIDKGKATYQDINNYRNIVELTETHRTASFVEKFANFFKMLWLRLKAMKHYKALNKE
                     SKKQEFENSFKDVQKIMRIVNHNIILRLKEEQNSTNVLEVSLVINHYYDMSRSLKWRA
                     QRRKERQENSNQIIPQAMFHNHKLEALYLQRHLLDELIRKNKINNIVAAQIRENINYN
                     EIVLSLQSKH"
     gene            complement(23762..25171)
                     /locus_tag="P6L01_000561"
     CDS             complement(23762..25171)
                     /locus_tag="P6L01_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDP3029652.1"
                     /translation="MARKLHRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLF
                     TYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSM
                     SDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIA
                     MVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGV
                     TAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIG
                     VPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNKTNSHGVPYLSML
                     VSSIALLIAALLNYIFPNAIQLFIYVTTLSTVLFLVVWAMIIVAYLMYLKKHPEAHKN
                     SKFKLIGGKPIAYIILAFFFFVFILLFFSDETRAAIYISPFWFIFLFFFYKKYKTNAE
                     KLAYEQRQNDSGHFRYDNQ"
BASE COUNT         8468 a         3681 c         4808 g         8305 t
ORIGIN      
        1 cggtagggaa cgtagtgtgt tccactgctg atttcttttt gagcattata gacggtatgt
       61 tggaagaaaa gaacaaaagc ttgtgtacga tagcatatag gtattaagaa tacgatgaac
      121 tcgttggcaa tattgacgta ctcaagtgcg tacgctataa ttagcgtaac agaaaacaaa
      181 ggagggttta tatgattatc actagcccta cagaagcgag aaaagatttt tatcaattac
      241 taaaaaatgt taataataat cacgaaccaa tttatattag tggcaataat gccgaaaata
      301 atgctgtgat tataggttta gaagattgga aaagtataca agagacaata tatcttgaat
      361 ctactggtac aatggacaaa gtaagagaaa gagaaaaaga taatagtggt acaacaaata
      421 tagatgatat tgattgggat aatctttaat gagcaattac acggttaaga ttaaaaattc
      481 agcgaaatca gatttaagga aaataaaaca ttcttattta aagaagtcat ttttagaaat
      541 tgttgagact ttaaaaaatg atccgtataa aataacacaa tcttttgaaa aattagagcc
      601 taaatattta gagcgatatt caagaagaat taaccatcag cacagggtcg tctataccgt
      661 agatgatcga aataaagaag tattaatact atcggcatgg tcacattatg attaatgaat
      721 attcaatatc tgaataactt taatggtaag ttaattaaag aaactagtat ttaagtgtag
      781 ggaaaatagc gacgttaatg cgttgttatc tctacacttt ttaattttat aatagcgcaa
      841 gactaaacaa attgaaatta gtaacaataa aagaataaca tattataata aggaatttta
      901 aattgtgact ttttcggaat attaaatttt aaaaatatga ggtttttgaa gcggatttct
      961 cacaaaattt taaaaaattt aagcctgaaa atgataaagc ggtagggaac gtttttctga
     1021 aagttagtga tacaatagtt ttaagttgaa atacaggagg atgaataaca tgaatcagtc
     1081 agtcaaatta cttaaacatt taacagatgt aaacggcatt gctggttatg aaatgcaagt
     1141 taaagaagca atgcgtaact atatagagcc tgtcagtgat caaattattg aagataactt
     1201 gggtggcatt tttggaaaga aaaatgctga gaatggtcaa tactcaatta tgatttctgg
     1261 tcatatggat gaagttggtt ttatggtaac aaagattgat aaacatggtt ttatttcatt
     1321 tacgccagtt ggtggatggt ggaatcaagt catgctatct caaaaagtaa cgattacaac
     1381 agattcgggc aaagaaatta gaggtatcat cggttctaaa ccgccacatg tcttaacgcc
     1441 tgaagaacgt aaaaagccaa tggaaatcaa aaatatgttt atagatattg gtgttagtag
     1501 caaggaagaa gctgaagaag ctggcgttga agtaggcaat atggtaacgc catatagtga
     1561 atttgaagtg cttgcaaatg ataaatattt aactgcgaaa gcatttgata atcgctatgg
     1621 ctgtgcatta gctgttgagg tattaaaacg tttaaaagat gaaaatattg gaattaactt
     1681 atacagtggt gccacagtgc aagaagaagt tggtttgcgt ggtgcgaaag tggcagcgaa
     1741 tacaattaaa ccagatttgg cgatagctgt cgatgtaggt attgcttatg ataccccagg
     1801 tatgtcaggt caaacgagcg atagtaaact aggcggtggt ccagttgtca ttatgatgga
     1861 tgctacaagt attgctcacc aaggtttgcg taagcatatt aaagatgtag ctaaggaaca
     1921 taacatcgaa gtacaatggg atacgacacc aggtggaggt acagatgcgg gaagtattca
     1981 tgtcgcaaat gaaggtattc caacgatgac aatcggtgtt acgctgcgat acatgcattc
     2041 taatgtttca gtgctcaatg tagatgatta tgaaaattct atccgtcttg ttactgaaat
     2101 tgtccgttca ttgaatgatg aaagttataa aaatatcatg tggtaatcaa atccataaat
     2161 aataaagaat ccttttaata tggtaggttg ttaaacaatt gtctaacttt aattcttagt
     2221 cattagacag tatccatgtt aataggattt tttgttttta atttaaatgc tgaaaatcaa
     2281 ttatgcctaa attttgatat tacaagaaaa tgattttttc ttaaatgtaa ttgcactaaa
     2341 aaccaaaaaa acgggaataa tatacctgat atattacatg aggagcggtg caaatgttgt
     2401 tagaaattaa agatttagtg tataaagcga gcgatagaat catactagat catatcagtc
     2461 taaaagtaga taaaggcgag agtattgcca ttataggtcc atcaggtagt ggtaaaagta
     2521 catttcaaaa gcaaatatgt aatttgatta gtccaactag tggagaactt tattttaaag
     2581 gtaaacccta taatgattat gacccggaag aattgcgtca acgaatcagt tatttgatgc
     2641 agcaaagtga cttgtttggt gaaacgattg aagataacat gatattccca tcacttgcac
     2701 gtaatgataa atttgataga aaacgtgcaa agcaattaat taaagatgtc ggtttgggac
     2761 attatcaatt aagttcggaa gtggaaaata tgtcgggtgg tgagcggcaa agaattgcta
     2821 tagcgcgcca actgatgtat acaccggata ttcttttatt agatgaatcg accagtgcat
     2881 tagacgttaa taataaagaa aagatagaaa atatcatttt taagttagtg gaacaggatg
     2941 ttgctatcat gtggattacc catagtgatg atcaaagtat gagacatttt caaaagcgca
     3001 taacgattgt tgatggcaaa atttctaagg ttgaggagtt gaatcaacat gagtaatacg
     3061 gcacttggat tgacggcatt gcttttagtc attccgatta tcatttcata taaagaaggt
     3121 ttacatatta ttaaagattt aattgttgcg acactacgcg cagtggtgca attaatcatt
     3181 ttgggatttt tgctgcatta tatttttaaa ataaacgata aatggctgct tattttatgt
     3241 gtattggtca ttattattaa tgcatcatgg aatacaatta gtcgagcatc accagtgatg
     3301 catcatgtgt tttggatatc atttctagct atcttcattg gaacagcatt accacttgca
     3361 ggtactgtag ctacaggggc aattcatttt acagccaatg aagttatacc tatcggcggc
     3421 atgcttgcaa ataatggctt gattgcaatt aatttagctt accagaattt agatcgtgca
     3481 ttcgtacaag atggtactaa tattgaatct aaattatcac ttgcagctac acctaaattg
     3541 gcttctaaag gtgcaatacg tgaaagtatt cgtttagcta tagtgccaac aattgattcg
     3601 gttaaaacat atgggcttgt gtcgattcct ggtatgatga caggcttaat tattggtggc
     3661 gtaccacctt tacaagcgat taaatttcaa ttgttagtcg tgtttattca tacaactgcg
     3721 accattatgt ctgctttgat tgcgacatat ttaagctatg gtcaattttt caatgcaaga
     3781 catgaattag tagcacgaaa tacggatgtt aagagtgaat catgatagat tttactgcat
     3841 cagatttagg cattagtttt aattggaaat gaagtgacgc gcacatatag tatcgctatt
     3901 cattaacaca gcgaaaatat tcataaaggc acgcatactt tgtagtcagt tatctgttct
     3961 gacatataaa gcgtgcgtgc ttttttgaag acattgttga aactgaagta attatacata
     4021 attattaaat gacatacttg tgttaatttt tcaaatactg aaaaacaatt tcaataattt
     4081 tccatttaag cacagaaaat taaagcaaaa tattatataa tagaacggtt atatataaaa
     4141 ttgtgttgca cacatttttt aataaatcgt tattctaagg gaaatgaata tcggaaattt
     4201 tgtttgaaag gagttttaaa atgtcaatca tgcgactatt tacattcatt ttaagtattt
     4261 ttatcgtagg aatggttgaa atgatggttg caggaattat gaacttgatg agtcaggact
     4321 tacatgtatc agaagctgtc gttggtcaat tagtgacaat gtacgcttta acatttgcga
     4381 tatgtggacc tattctggtt aaattaacga accgtttttc atcaaggcct gtattattat
     4441 ggacattact tatatttatc attggtaatg gcattattgc tgtagcgcca aatttttcaa
     4501 tattagtagt tggtagaatt atctcatctg cagcagcagc actaattatc gtaaaagtat
     4561 tagctattac agcgatgtta tcagcaccta aaaatcgtgg caaaatgatt ggacttgtct
     4621 atacagggtt tagtggggcc aatgtttttg gtgtaccaat tggaacggtt atcggcgatt
     4681 tagtaggttg gcgctataca tttctattct taattattgt gagtattatt gttggcttct
     4741 tgatgatgat ctatttaccg aaggatcagg aaatacaacg aggccctgtg aatcatgagg
     4801 caccatctca tgaaaatcat gttacttcga aaatattaag acctgctgaa gtagcaaaat
     4861 atttaattat tacgttttta gtattgattg caaactcagt gacattcgtc tttataaatc
     4921 cacttatttt atctaatggt catgatatgt cattcgtttc attagcactt ctagtaaatg
     4981 gaatcgctgg cgttattgga acatcattgg gtggtatatt ctccgataaa attacaagta
     5041 agcgttggtt aatgatttct gtttctattt ttatcgttat gatgttactt atgaatttaa
     5101 tcttacctgg ttcaggtcta ttgttagcag gactatttat ttggaatatc atgcaatgga
     5161 gtactaatcc agcagtgcaa agcggtgtga ttcaacatgt tgaaggcgac acaagccaag
     5221 taatgagttg gaacatgtct agtttaaacg ctggtattgg tgttggcggc attattggag
     5281 gcttggtcat gacacatgtt tctgttcaag ctatcacata tacgagtgcc atcattggcg
     5341 cattaggatt aatcgttgtt ttcacattga aaaataatca ttatgctaaa acatttaaat
     5401 catcataatt ctcatatgaa aagcacgcct gctatcaaat tcaggtgtgc ttttttagat
     5461 gcgataacgt tattgatatg tgcgataata gcgacgttca ttatgataca tcggccaagg
     5521 cattttaccg cttttagcaa aattagctaa atcattttgc atttgtcgac ttaaaaattt
     5581 aaggtgagca gttgttggat attgatgtgc tgctaatatt tgtaagtttc cgaaccaaaa
     5641 taccatatct agaatatggt aagcactgcg atagtgtgcg ctagaagtat catgccatgc
     5701 aaattcagca agccatagtt gtgcgtttga atcttgtata ttaagatgtt gtaaaaattg
     5761 cttcatcggc tgtttgaaat aacaatgtgt aatcgctaaa gattgttgct tcgcagtctg
     5821 aacatcttcg tattttaaag gaatatcatt taattccata atgtcgataa agcgttgcgg
     5881 cgataatttt ttctgttcac tagtaatata aatatcgcct tcatcttttg tataacatgc
     5941 aaaaattggt ttcgttgttg gataatcatt ttgtatataa tctgttttaa taggcgcata
     6001 tattaaatca agccctttag aaggtcctcg agattgtttt agcttcgcca tcagcataag
     6061 aatatcatct gtcgataatg atgtaacatc atctgtatct aaataatcgc gcatcatttt
     6121 ttggaaatat tgtgctttat tgcgtgcact cttaagggtg tctaatcgta gtgcgccact
     6181 tagtagaacg actttatgga agtatggctc aatgtcaggt attttaagta aagtcaaaat
     6241 gctcatactg cctgcagact gacccattaa agtaatgtta ttagcgtcgc caccaaagga
     6301 ttcaataaat tgatgcaccc attttatgac attgatttga tctgaaagtc cattattgga
     6361 atgaaaatct ttattaaaat atgaccagtc taaatatcct aatgcgccta aacgataatt
     6421 gcatgtaata acgataatgt cgttattttg tactaaatgt gccggttgat agagttcggc
     6481 tgtaccatga ccattttcaa aactaccacc ataaaaataa atgatgacag gtttcttcgt
     6541 ctgatcatta tgttgtttcc aaatatttag atataaacag tcttcatgtt cagtaaaagt
     6601 tgtagattgt gaggagaaaa aatcttctaa tttgttgtct ggttgcggtg ggatgggttg
     6661 tatttcagtt gcatcaattg gctctgacca ttgtgttttt aacgtagaat gtttaaagcg
     6721 attatcatga actggtggtt ctgcataagg aatgcctaag aagatatcta aaccatcttg
     6781 tgtaatacca tgaatttgac cacctgtagt attaatcttc atatttatta acctccttta
     6841 tcatccccaa tttaatcata ccacggtcta tagatgatag gtgacaagta taacaagaca
     6901 attttgtgac tgaatcagac tagcttgata tctgttttaa ctcaaaccat tttcagcatt
     6961 atatgatgtt cttctacagt gacatttcat tacaatatca ctctcgccac attgttatgt
     7021 tagttattta taaccgattt tcaaaataaa aaaccaacca agatgttgaa atcaatggtt
     7081 ggttaatgtg tgtgtgttga aatcatatat ggcattgcat tttaaaatat ttaatgagat
     7141 gactttgatt cattatttag tgactcgcgc atctttgtat tgattcgtgt tgcgcgacgg
     7201 aaatagacgc tttcaaatga actcgttgta ccgatgtaat agaatataag cgccacaaca
     7261 ataataacga taaagtcaaa tggatagtga atcatattta agcctttaaa ttctttgctt
     7321 cctataaatg acagtattga tagcacgatt aaatatacaa taatccataa gctaccacca
     7381 atttgtttct ttgtattacg ccaattcata cggtattcat agaagaagta gattggtaat
     7441 ccaagtataa tgattaaaat aacttcagcc gttgttggcc acattgccca atatatagct
     7501 aatgaagcta atacaaatga taatggtgcc attactttta aaatttttgc tctaaatgga
     7561 cgagtcattg ttggtcccat ttttcttaat gcaatcactg tcgttgggcc agttaaatag
     7621 gctactaaag ttgcagtaga aataactgct gctagcgtac cccaatctct aaataatgta
     7681 accataatca tactaatgat ggcattaaag atgattgcta cacgtgggat atgatatttt
     7741 tcattcatct tcccaagaaa tttagggata tgtccatttt tctccattgc tcgtaaaact
     7801 cgacctgtaa cggcgacaaa tgacacgcca gtaccgaatg gtgatacaaa agcttcaata
     7861 tatagtaaaa ttgcgagcca attaattcct aataagatag ctaaatctgc aaatggtgaa
     7921 ttgaagttga tgccattcca tccactatgt tgtaacattg attgaggcat agaagtgata
     7981 aacgtacttt gtaaaatgat gtacaacact gcactgattg acagtgaaat agcgatgcct
     8041 cttgcgatat ttttttcagg atttttaatt tctgaaccca tattaataat tgtctggaat
     8101 gaattgaatg aaaaaataat ccctgatgct gttgttgcag caaaaatcgg tgcacttccg
     8161 taaggcataa atgtgcttgc cgaatggccg taatttgaag tgtcgaatcc tgataacatc
     8221 aacatgatga tggttaacat gggtacgcct aatttaaata cagaaattaa actcgtaaat
     8281 gatgttaaaa gttttacgga ccaatagttt aataatgaaa aaataacaat gatgagatat
     8341 acagctagca atccgtatgt gctaatagag ccattttcca ttaaatatct cattggtttc
     8401 gcccaatgcc acggccaaga actcatatat tgaacagctg acacagcttc gataggtatt
     8461 attgtcacca aagacaccca attcgcccaa gcagcaataa agcctaataa tgagccatgt
     8521 gtatactggg catagttact catgccacct gattgaggaa acattgtgcc gatttcaatg
     8581 tagttatagg caatggttcc aatgactagg aatccaagaa cccatgatat gattgctgct
     8641 ggtccagcta ttgatgatgc ttcccacgca ccaaatagcc aaccagatcc tattaaagag
     8701 cctaacccta ataagactaa ttgggataga ttgattttgt cgttgtgcaa ttgcttgtct
     8761 ttattcataa atactccttc gttattgcca aaaatacaaa aaataatatg cgacaattat
     8821 acgcttatta taataatagt caaatgcatt ttttgtaaga acgcaaaagc atttgtaaaa
     8881 ttagtttttt ataaaattag gatatataat acaatgtgta ttgaaaaaca aaatggaagt
     8941 gatgactatg agtgagaaac aatcaaatga acaagttgaa gcatttttaa ataaagagag
     9001 tcaatggcaa gaagaatata aatatttgag agcgttaatc tttaatgaaa cagaattaga
     9061 ggaagcgtat aaatggatgc atccttgtta cacgttgaat aataaaaatg tagtacttat
     9121 ccatggcttc aaaaattatg ttgcactatt atttcataaa ggtgccattt tggaggataa
     9181 atatcataca ctcattcaac agactgaaaa ggtgcaagca gctcgtcagt tacgatttga
     9241 aaatttaaca gagattcaag cacgtaccga agaaattaaa tattatctag ccgaagcaat
     9301 taaagctgaa aaagctggta aaaaagttga aatgaagaaa acagaggaat atgttattcc
     9361 taaagagtta gaagctaagt tcgaagaaat gccacagtta gagtcttcat tttataaatt
     9421 aacgccaggc agacaacatc aatatatata tcatattgga caagctaaac gcagtgaaac
     9481 aagacaaaag cgtgttgaaa agtatattaa ccaaatacta gaaggtaaag ggatgcatga
     9541 taagtaatta atgagtaaag cataccggtt atacaacaac atacaagatg acacgaaaca
     9601 accaatggct catgctgttg gttgtttttt taggtgtgtc tgtcatgggc aacactttga
     9661 cgttggaatt ccgttacagg cttgggagta gaaaatgtta gcaaaaggca agggtgtcta
     9721 caatgaatga tgaagatatt aaaatataag gatgactttg tgagtggcgg atgggcggtt
     9781 gtccgtctgt aacaatggat gcgtgtgcat tattacaaaa attcgacttt tgtaataata
     9841 tttcacattt tcgacacttt tttgctataa aacaaccaat tgagcgataa taaattcgct
     9901 tttaaaaaat atgagttatc tatttagttg ccaaagataa aataataatg tttaataaca
     9961 tcatatagag tatgttagtt ttaaatgtcg aatatacgaa tgtgcaaaca aagtaatcgg
    10021 tagaaattca acatacatag cgccgtttac tgttaagtat tcacattaca gatgaaaaat
    10081 ataaaattct acataatcaa gaccatgatg tgtacttgtt taacttatga ctctatttgt
    10141 ttaacaattg cgataatggt ctttttattt tatgcgtatc attcgtcata ttttttatga
    10201 ggaaggagaa atgattatgt taagtattaa gcatttaacg aaaatttatt ctggtaataa
    10261 aaaggcagta gatgacatct ctttagatat tcaatctggg gaatttatcg catttattgg
    10321 aaccagtgga agtggtaaaa caactgtttt aagaatgata aaccgtatga ttgaagcgac
    10381 agaaggacaa attgaaattg atggtaaaga tgttcggagt atgaatcctg tcgaattacg
    10441 tagaaatatt ggttatgtta ttcaacaaat tgggttaatg cctcatatga cgattaaaga
    10501 gaatattgta ttggtaccca aattgttgaa atggactaaa gaagaaaagg ataaacgtgc
    10561 aaaggaatta attaaacttg tggatttacc ggagtcattt ttagagcgtt atccagcaga
    10621 actatcaggt gggcaacaac aacgtatcgg tgtcgtaaga gcacttgcgg ccgaacaaga
    10681 tattatttta atggatgaac cttttggtgc attggatcct attacgagag atacgttaca
    10741 agatttagtt aaaacgttac aacgaaaatt aggcaagacg tttatctttg taacacatga
    10801 tatggatgaa gcgattaaat tagcagacaa aatttgtatt atgtcagaag gtaaggtggt
    10861 gcaatttgat acaccagaca atattttaag acatcccgca aatgattttg tacgtgattt
    10921 tataggacaa aatagactga ttcaagaccg tcccaatgac aagactgtag aaggtgtaat
    10981 gattaaacca atcacgatac aggcagatgc aacgcttaat gatgctgttc atattatgag
    11041 acaaaagcgt gttgatacta tttttgtagt agatagtaat aaccatttac taggtttctt
    11101 agacattgaa gatataaatc agggtatacg tggacacaaa agtttacgag acaccatgca
    11161 acaacatatt tataccgttc aaattgattc taaattacaa gattctgtac gtacgatttt
    11221 aaaaagaaac gttaggaatg tacctgtcgt agatgataag caacgtttag taggactgat
    11281 tacgcgtgcc aatgttgttg acattgtata tgacacgatt tggggcgata gtgaggatgc
    11341 agtgcaaaca gaacatgtgg gggaagacac tgtgtcctca aaaatgcatg agcaacacac
    11401 tactaatgtc aaagtacgtg acataggaga tgataaatca tgattgagtt cctacatgaa
    11461 catggtggac agttgatgtc gaaaacactg gaacattttt atatttctat agtggcatta
    11521 ttacttgcca tcattgttgc agtacctata ggcattttat tatcaaaaac aaagcgaact
    11581 gccaatattg tattaactgt ggcaggtgtc ttacaaacta ttccaacact agctgtactt
    11641 gctattatga taccgatttt tggtgttggt aaaacgcctg caattgtagc gctatttatt
    11701 tatgtattat tacctatttt aaataacacg gtactcggtg ttcaaaatat tgatagcaac
    11761 attaaagaag ctggaaaaag tatgggaatg acacaatttc aattgatgaa ggatgttgaa
    11821 ttgccgttag cattgccgct tatcattggt ggcattcgtt tgtcatctgt gtatgtaatt
    11881 agttgggcta cacttgcaag ttatgtaggt gcgggtggat taggtgattt cattttcaat
    11941 ggtttaaatt tatatgatcc actgatgatt gtaactgcaa cggtactcgt tactgcacta
    12001 gcattaggtg ttgatgcctt attagcttta gttgaaaaat gggtagttcc caaaggctta
    12061 aaagtatctg gataattagg aggctaagat aatgaagaaa attaaatata tacttgtcgt
    12121 gtttgtctta tcgcttaccg tattatctgg atgtagtttg cccggactag gtagtaagag
    12181 cacgaaaaat gatgtcaaaa ttacagcatt atcaacaagc gaatcgcaaa ttatttcaca
    12241 tatgttacgg ttgttaatag agcatgatac acatggtaag ataaagccaa cattagtaaa
    12301 taatttaggg tcaagtacga ttcaacataa tgccttaatt aatggggatg ctaatatatc
    12361 aggtgttaga tataatggca cagatttaac gggagctttg aaggaagcac caattaaaga
    12421 tcctaagaaa gcaatgatag ccacacaaca aggatttaaa aagaaatttg atcaaacgtt
    12481 ttttgattcg tatggttttg cgaatacgta tgcattcatg gtaacgaagg aaactgctaa
    12541 aaaatatcat ttagagacag tttcagattt agcaaagcat agtaaagatt tacgtttagg
    12601 tatggatagt tcatggatga atcgtaaagg cgatggctat gaaggattta aaaaagagta
    12661 tggttttgac tttggtacag tgagaccaat gcaaataggt ctagtctacg acgcattaaa
    12721 cacagagaag ttagacgttg cattaggtta ttctacagat ggtcgaattg ccgcgtatga
    12781 tttgaaagta cttaaagatg ataaacaatt tttcccacct tatgctgcga gtgctgttgc
    12841 aacaaatgaa ttattacggc aacacccaga acttaaaacg acgattaata agttgacagg
    12901 aaagatttcg acttcagaga tgcaacgctt gaattatgaa gcggatggta aaggtaaaga
    12961 acctgctgtc gtcgcagaag aatttttgaa aaaacaccac tattttgata aacagaaagg
    13021 tggtcataag taatggaagg taatttatta cagcaattat tcaattatta tgttacgaac
    13081 tttggttatc tatgggattt atttttcaaa cacttattaa tgtctgtcta tggtgtgctg
    13141 tttgcagctt taattggtat tccattggga atcttgcttg caagatacac aaaactttct
    13201 ggatttgtaa ttacaattgc aaatataatt caaacagttc cagtcattgc aatgttagct
    13261 attttaatgt tagtcatggg cttaggttca gaaacagtag ttttaacagt gtttttatat
    13321 gcgttacttc caattataaa aaacacttat actggtatag ctagtgttga tgcgaatatt
    13381 aaggatgctg gcaaaggtat gggaatgaca cgcaatcaag tgctacgaat gattgaatta
    13441 ccgttatctg tttcggttat tatcggtggc attcgtattg ccttggttgt tgcgataggt
    13501 gttgttgccg ttggatcatt tataggagca cctacgcttg gtgacattgt gattcgtggt
    13561 acaaatgcga cggatggcac aacgtttatt ttagcaggtg cgattccgat tgctatcatt
    13621 gcaatcgtca ttgatgtact attaagattt ttagaaaaac gattagaccc aacaacacga
    13681 catcgtaaaa atcaatctaa tcatcggccg caaagtatta atatgtaata gtagaagatg
    13741 tttataattt agcgatttcg tttcatgatt tataaaaaat gaggctactc aaggagctca
    13801 aataatcttt gagtagcctt tttataggtt gtgtttgtat gcgtttacac taaaatagca
    13861 attattatca tgaaagtttt tggataaaaa gcgttaatta ttgtaaaaat gctaaaaaat
    13921 gagatgtttt atttataatt ttctgcaaat ttatgatatt gtttcttaat atatcatatt
    13981 aaaaatttgt ttttcttaaa cataggaggc ttatctaatt catggacaca tcaaaacaat
    14041 ttagaggtga caaccgattg cttttgggta tcgttttagg ggttattacc ttttggctat
    14101 tcgcgcagtc acttgttaat cttgttgtcc cattacaatc aacatatagt agtgacgttg
    14161 gaacgataaa tatcgctgtt agcttatctg ccttatttgc tggtttgttt atcgtaggtg
    14221 ctggtgatgt tgctgataaa tttggtcgcg tcaaaattac ttatgtagga ttgatattaa
    14281 atgttgtagg ttcattactc atcatcatta cacctttgcc agcattttta attataggta
    14341 gaataattca aggtttgtct gcagcatgta ttatgccatc cacacttgct attattaacg
    14401 aatattatat tggtacaaga agacaacgtg ccttaagcta ttggtctatt ggttcttggg
    14461 gtggtagtgg tatttgtacg ttgtttggtg gcttaatggc tacatatata ggttggcgtt
    14521 caatatttgt tgtttcaatt ctattaacat tattagcaat gtacttaatc aaacatgcac
    14581 ctgagactaa agcagaacca atcaaaggta tgaaagcaga agctaaaaag tttgacgtta
    14641 ttggtttagt cattttagta gtgacgatgt taagtttaaa tgtaatcatc acacagacgt
    14701 ctcattttgg tttagtttca ccgttaattc taggtttaat tgttgtgttt atctgttcat
    14761 tagttggttt tgtatattac gaaaataaaa taaagcatcc acttgttgat ttttcaattt
    14821 ttaaaaatag aggatacagc ggtgcaacta tttcaaactt cttattaaat ggtgtagcag
    14881 gtggtgcact tatcgttatt aacacgtatt atcaacaaca attaggattt aattcttcgc
    14941 aaacgggtta tatttcatta acgtatttaa tagcagtgtt gtcaatgatt cgtgtaggtg
    15001 aaaagatttt atctcaacat ggtccgaagc gcccactatt actaggaagt ggctttacag
    15061 tgattgggtt aatcttattg tcgttaacat ttttaccaga agtgtggtat atcatatcta
    15121 gtatagttgg atatttattg tttggtactg gtttaggatt atatgctaca ccatcaactg
    15181 atacagcagt tgctagtgcg ccagatgata agtcgggtgt tgcttcaggc gtatataaaa
    15241 tggcgtcatc attaggaaat gcatttggag tagcagtatc tggtacagtt tatactgtgt
    15301 tagcagctaa tttaaatttg aacttaggtg gtttcacagg tatgatgttt aatgccttgc
    15361 tagcaattgt tgcattttta gtcattttac tattagttcc taaaaatcaa acgaatttgt
    15421 aaaaccgaaa tgaaagcaag ttattatgta gggattttaa aggaaatttt gtgaaagtaa
    15481 gtttatcata cacacttaat gttacgtatt gacgtttaat gttaggtgtg ttcttttata
    15541 gacgataaaa gctgtgtgca tattaagcga atgattttca aattgacgct aatatgcgaa
    15601 agtagtattt ttaaaatgaa caacaacgat gaagaggggt ttataggatg aaaattgcaa
    15661 ttgctggatc gggtgcatta ggtagtggct ttggtgccaa actatttcaa gcaggatatg
    15721 atgtcacact tattgacgga tatacatctc atgttgaagc ggttaagcaa catggattaa
    15781 atataacgat taatggagag gcattcgagt taaacattcc gatgtatcat tttaatgatc
    15841 aacgggacga aagcatttac gatgttgtct ttctatttcc aaagtctatg caattaaaag
    15901 aagtgatgga agatatgaag ccacatattg ataatgaaac gatcgtcgta tgtacgatga
    15961 atggtctgaa gcatgaagaa gtcattgcgc agtatgttgc tcaatcacaa attgtcagag
    16021 gtgttacgac ttggacggca ggtcttgaaa gccctggaca cagtcattta cttggtagtg
    16081 gaccagttga aataggtgaa ctagtggatg aaggtaaaga aaatgttata aaagttgctg
    16141 atttacttaa cgaagcggaa ttgaatggtg tcattagtaa agatttatac caatcgattt
    16201 ggaaaaagat ttgtgttaat ggtacggcaa atgcattaag cacagtgttg gagtgtaata
    16261 tggcatcact gaatgaaagt agttatgcga agtgtttgat ttataaatta acgcaagaaa
    16321 tagtgcatgt agcgacgatt gataatgttc atttaaatgt tgatgaagta tttgaatatt
    16381 tagttgattt aaatgaaaaa gttggtgcgc attatccatc catgtatcaa gatttaattg
    16441 ttaataatag aaaaactgaa attgattata ttaatggcgc agttgcaaca ttgggtaaac
    16501 aacgtcatat tgaagcgcca gtcaatcgct ttattactga tttaattcat actaaagaaa
    16561 gtcagcgaca cgcacaggat taattggtgt tttaggtatg attacaagga aatgacaatc
    16621 acgtgatatt acggtcatta ttaagattga aatgtaataa ataaagaaca gcagtaaggt
    16681 actttcaaat tgaaatgatc ttggtgctgt ttttcttgat tgatcttcgt cataattcag
    16741 atttgtcata ggtctacgac atactattag tatttactag acagttttta cgacgacact
    16801 ttgaaaaatt ttgaggcaaa tcatttggaa gtctcacgtg aattttgtaa actcatcaag
    16861 caagtaatta tattaaaaag acaaatagag aaaaggtgtt tataatgagt aaaatttttg
    16921 taactggtgc aacgggcctt attggcatta aattagttca aagactaaaa gaagaggggc
    16981 atgaggttgc tggttttact acatctgaga atggtcaaca aaagctagct gctattaatg
    17041 taaaagcata tattggtgat atattaaaag ctgatactat tgatcaagcg ttagcagatt
    17101 ttaaaccaga aatcattatc aaccaaatta cggatgtaaa aaatgttgat atggcagcaa
    17161 atacgaaagt acgtattgaa ggttctaaaa acttaattga tgcggcgaaa aagcatgacg
    17221 ttaagaaagt aattgcccaa agtattgcct ttatgtatga acctggcgaa ggattagcaa
    17281 atgaggaaac ttcacttgat tttaactcaa ctggtgatag aaaagtaacg gttgatggtg
    17341 tggttggtct agaagaagaa acggctcgta tggatgaata cgttgtttta cgttttggtt
    17401 ggttatatgg cccaggtact tggtacggaa aagatggcat gatttataat caatttatgg
    17461 atggtcaagt gacactttca gatggcgtaa catcatttgt gcatcttgat gatgcagttg
    17521 aaacatctat tcaagctatt cattttgaaa atggtatcta taatgtagca gatgatgcac
    17581 ctgttaaagg ttctgaattt gcagaatggt ataaagaaca acttggtgtt gaaccaaata
    17641 ttgatattca acctgcgcaa ccatttgaac gtggcgtaag caatgagaag tttaaagcgc
    17701 aaggtggtac tctgatttat aaaacttgga aagacggcat gaatccaatt aaataataat
    17761 ttatccgttt aatatacaaa gaataaagac ttggtcgaat cgtggatgat atattatcaa
    17821 acgcacggct cgaacaagtc ttttttatta tgtcttcgtt atctttgtat gaaggaataa
    17881 cagaattaca attaatgtac tgaataatgc aattaatgtt gtgattagtg ctaatttaat
    17941 ttctattggt agccaagtca gtacaaaaga ccaattattg ctaccgagaa tgagatatgg
    18001 taatgcatat aatatgagcg ctatagcgat acataaacat aaagttagcc aactcaatac
    18061 agcaatccgt tttgatctac gcaaaagaat ttgaccatgg cgcatttgcc aagcacgata
    18121 agctgagaat ataaatgcta ataaaataag tgttgtcatc aaaacggtaa caatattaaa
    18181 ttggtcttta tattgattga gtatagacgc aaccgtcgaa tatcgcttgt gatttacaat
    18241 ttgtgtatta agatgctcaa ctaatttggg tacatattcc gaatttagat ttgcaagcac
    18301 aacaattcca taattttgtt ttggatttag taaaataaat gatgaaaagt tatcgagcgt
    18361 tcctgaatga aacactaaat gttcatcatt attggtaaac cagcccgaag catatgcatt
    18421 ggcattaggt tcaccaattg ttgatgataa atttttatgt gattgttgaa ctaatgattt
    18481 gtatttatca ggtggattaa gttggaattt tatccaatgt tccaaatctt cagttgatgt
    18541 catcatatat gctgatggtg tatcccaaag gttaaattca ggtttagaga cgacaggtgt
    18601 cgaaccttgt aattcgtagc caatagcatc atgttttgat ttgttattgg tttgtttgaa
    18661 tgatgtatgt gtcatatgca aaggcttgag ccatgaattt gtaatatatt ttgtatagga
    18721 ttgcttcgta acgttttgga taattaaacc taataaatca tagttcatat ttgagtattc
    18781 aaattcttct ccgggcttat gatgtaattc atcacccata attgcacggg ttacatcatt
    18841 taaacgatta tttttatttg ttacggcgtc ttcgcttgta atatcactag gtataccact
    18901 tgtttgagcc aaaagttgct taatcgtaat agtttcattt tgaccattat agttcatttt
    18961 aaaatgaggc acatgtttgg atacgtcatc atttaagttt aatcgacctt cttgagctaa
    19021 ttttaaaatt gcaagacctg tgaaagcttt agtattagaa gcaatttcat actttgttgt
    19081 gggagaagct ttgacttttt tatcaacatc ttgataacca taacctttat ttaaaaaaac
    19141 tttgccattt tttactatta aaattgatgc tccaggaatg tgtccctttt gtaaatcatg
    19201 ctcgataatt ttatctattt gttgttgcga atcattggtt aaccgtgtct tcgtattgct
    19261 atttaatgtt atatatatag caattgaaat ggcgactaaa atgataatag aaatggatag
    19321 aaaatacagt tttttagtag tcataaacgt tcctttttat atgttaaatt tacttaagtg
    19381 cctaatgaga tttattttat tataccagtt ctattgaata aattaattaa agaggtatat
    19441 ttaccattat ttttgacacc ttatatgtta ataggtgtat aattacataa aaaatattaa
    19501 ttaactctct atactttttg gaaagatagg cgtatagtga tatccatcaa aaactataaa
    19561 aaggaatagg agattatgtt caatcaattt aaaagactta ttatagggca acctaaaaaa
    19621 aacagagaac taaaagacga aaagatatcg aagtttaaag ggttagcgat tctttcgtcg
    19681 gatgcattat cttcagtggc ttacggtccc gaacaaatac tgattacatt atcagtagtg
    19741 ggtgcagttg caacttggta tactttaccg attgcaggtg ctgttctgat cttattagct
    19801 gctttaatta tgtcttatag gcaaattatt tatgcatatc ctaaaggtgg cggcgcgtat
    19861 atggtgtcca agaccaattt aggagaaaaa tggggattgc ttgcaggagg ctcattatta
    19921 gtcgattaca tactgactgt cgctgttagt atatcatctg gtgctgatgc atttgttgct
    19981 gcattcccaa gtttatatgg gcataaagta ctgattgcat gtttacttgt actttttatt
    20041 ttaatattaa atttacgtgg tttaactgaa tctgccaccg tattatccta tccagtatat
    20101 cttttcatta tagggttagt gatattaata tttataggta ctttccgtgt ggcgacagga
    20161 gatattcaac cacatatgca tgcatcagta ggaactgcgg ttcctggagt aacattattc
    20221 ttgctattaa aagctttttc atcaggggct tcatcattaa caggtgttga agctatttca
    20281 aatgcggtaa ccaattttag agaaccaagt gctaataatg ctgtaaaaac gttaattgct
    20341 atgggttcaa ttttagcgtt tttattagtt ggtatcgttg gtttagcata tgtttatgga
    20401 attatgccac aaacggaaac gacagtttta tcacaattag ctatgcaaat atttggtgat
    20461 aatgctgcct tctattttgt acaggcaacg accgtgatga ttttggtatt agcagccaac
    20521 actggattta cagcttttcc gatgttagcg gcttcaatgt ctaaagataa atatatgcct
    20581 agaatgttta ctgttagagg tgatcgctta ggctactcta actcaattat tattttaggt
    20641 gtattagcca ttattttaat tattgtattt gatggtatga ctgaagattt aattccattg
    20701 tatgctgttg gtgtatttat tccatttaca ctagcacaat tcggtatggt tattaaatgg
    20761 attcatgaac gtcctaagaa ttggttaagt aagttgtctg tgaacttact aggtggtatc
    20821 gtgacattta ttgtttttat gattttactc ataactaaat ttagccaagt atggccgata
    20881 cttattttct tgccgttcgt ggtgattttc ttcttgaaaa ttaacaaaca ttatcgagat
    20941 atcgcagaac aattacgttc tgacattgat gttcttaatg tggacgtggt agatcgtaat
    21001 ttagcaattg tgccgataac aagtattaca acagcagttg ataagtctat ttattacgca
    21061 caaatgcttg cgaataatga tgtcatagca gtacatgtat catttggaga tgaagatgaa
    21121 aaagcattcc aagagaaatg gaaacgtcat ttcccagatg taagattagt tattttacat
    21181 tcggaatatc gcagtattat acgaccaatt tcacgtttta tcgataagat taatcgtaaa
    21241 gcaaacgatc aaaactatat gattacagta gtgataccag aatttattac taagaaacgt
    21301 tggcataatt tattacacaa tcaaactagt ttgcgtatga aattgtattt aatttatcaa
    21361 aagaatgtta acgtatgtac aattccattt aaacttaaaa aatagattct ataatcaaat
    21421 cggattgatg aaaggttatt tacttttcat caatccgatt ttttgatgtt gaatataaaa
    21481 accccgtaca tatgagatga taatgacttt gtctcatatg tatggggggt attgttatgt
    21541 cttgcttaat gtttggactg taaagacaag acaatttcgt tgtaattgat attttctcga
    21601 atttgagctg caacgatatt gttgatttta tttttgcgaa ttaattcatc taaaaggtga
    21661 cgttgtaagt acaatgcttc caatttgtgg ttatggaaca tagcttgcgg tatgatttga
    21721 ttgctgtttt cttgacgttc ttttcgacgt tgtgcacgcc actttaatga gcgactcata
    21781 tcatagtaat gattaatgac taagctaacc tcaagtacat ttgtactatt ttgttcttct
    21841 tttaagcgta aaataatatt gtgattcaca atacgcataa ttttttgaac atctttgaat
    21901 gaattttcaa attcttgctt cttagattct ttgtttaagg ctttgtagtg cttcatcgct
    21961 ttaagtctaa gccataacat cttgaagaag tttgcaaatt tttcaacaaa tgatgcagta
    22021 cggtgtgtct ctgttaattc gacaatattg cggtaattat taatatcctg atacgttgcc
    22081 ttacctttat caattaaacg ctcaagtgta gatgtttcga ttacttttgc aatatcttct
    22141 aaacgtttga gttcttttgt attttgatta ttaggttctg aatttaataa aaataacaat
    22201 tctccatagt attggtttaa tactgggcga taatttgtat cgtttttgtc ttttttactt
    22261 tcgtttttaa aatgctgtat cactttttga acaatgaaaa ttttggcaga ttgataagtc
    22321 atacctttaa aagtagtatc ttcggcagat ggtgtaatta aaggtaaaac aatttgcgct
    22381 aagattaaac taattaatac catgaaagat gcaataaaca ataaatcatt acggtattcg
    22441 aatgcttgtc cttttgtaat gataaatggt aatgtaagtg ccattgaaag tgaaattgta
    22501 ccgtgaatac cacacatcgt cataataaat gcgtaacgca cacgagaagg tggtgtttca
    22561 tgtgaatcat gttcttcgtc tagataagat tgtatatttt tcgggaaata aaaatctttg
    22621 taccagaaat aaacccaaac aaatcgacat gcataaattg caatagcgat taatatagtg
    22681 atcactatta aaaatgaaaa gttgtcaggc tctgtttgga aaatatctat gactacagta
    22741 ggaatcataa aacctaatac aacaaataca aaaccattta aggcataact aaatgtgttc
    22801 caaatttgat ggtaattcat ttgtagttcc gtttgtgctc taattaaacg gtcgcgttct
    22861 aaaccatgga ttagacctgc gattacaact gcaatgatac ctgaagcatg aacttcttct
    22921 gctaaaaagt atacgacaaa aggagttaat aattgaataa aagttaaggt attgttatct
    22981 tttaaacctt tattagcggt taagtctata cgtattctaa cgacaacgaa tccaataatt
    23041 gcaccaataa gtacacctag tattgttgaa atgataaatt gttcaacagc ttggaataat
    23101 gaaaaggtac cagttactaa tgcagtaaca gcaattttaa atgaaatgat acctgctgca
    23161 tcattgagta aagattcacc ttctaaaatc gtcatagaac ctttaggtaa taattttccg
    23221 cgtgtaatag cagatactgc tactgcatca gtaggacata aaattgctgc tattgcaaaa
    23281 gcggctggca ttggtaaggc aggccaaatc caatgtataa aatagccaac accgactaca
    23341 gttgcaaaca ctagtgccat tgacattaat agtataggtt tacgatattc taataatttt
    23401 gttcgagaga cgtgggtacc ttctacaaaa agtagtggcg cgataacggc aaacataaac
    23461 acttcagaat tgaattggaa atcaacttgt attggaataa tgaaaataac gacacctaat
    23521 gcaatttgaa taaaggcagt aggaatttgt gggaatcgat tattgataac cgaactaata
    23581 atcacagcaa aaataaaaat taaaaatgct tctaatagtg ccatacaata ctcctcaaaa
    23641 ttttaatagt taatatttta tcacttttaa ggcataatga catagatata ttgataaaat
    23701 gaagttattt tcaaaaaaac tctagtatcg gttgaactga tactagagcg agatgtttaa
    23761 attattgatt gtcatatctg aaatgaccgc tgtcattttg tcgttgttca tacgcgagct
    23821 tttcagcatt cgttttgtat tttttataaa agaaaaataa aaatataaac cagaatggtg
    23881 aaatataaat agctgctctt gtttcgtcac taaagaataa taaaatgaat acaaagaaga
    23941 agaacgctag aataatgtaa gcaataggct taccaccaat caacttaaat ttactgtttt
    24001 tatgtgcctc aggatgcttt ttcaaataca ttagataagc gacaataatc attgcccaaa
    24061 caactaaaaa caacacagtt gataacgttg taacgtatat gaatagttga attgcattag
    24121 ggaaaatgta gtttaacaag gctgctataa gtaatgcaat tgatgaaact aacattgata
    24181 aataaggcac gccatgacta ttcgttttat ttaaaacttt aggacctaac ccttgttgtg
    24241 acagtccgaa taagatacga ctattcgaat agataccact atttgtagca gaggccgcgg
    24301 cagttagcac gacaaagtta acgacgcctg ctgcaaatgg cacgccgatt aatgtaaaga
    24361 gtttaacgaa agggctacta tttggatcga tatcattcca aggtatgact gacataatta
    24421 ctaatagacc accgatatag aataataaaa tacggatagg tacattatta attgctttcg
    24481 gtaaggtttt ttcaggatct ttcgtttcac cagcagttac acctataagt tcaataccaa
    24541 tgaatgaata tacagcaatt tggaatgaca ttaggaaacc aaatgttcct ccagggaaca
    24601 tgccgccgtg actgattaag tttgtgaatg atgcatgtcc ataatgtgtt ttaaatgaga
    24661 aaaagattaa tacaagacca acgataacca tcgcaataat tgtgacaact ttaataatag
    24721 agaaccaaaa ctccagttca ccaaataatc tggcacctaa taagttgaag ctaatcaaga
    24781 ttaaaacaat aaatagcacg gtaatccaat ttgggacttg tggataccaa aatgcaaagt
    24841 attgtcccat cgcagttagg tctgacatac ttgatacaat ccaacataac cagtaagtcc
    24901 agccaatgac aaagccacca aaagggccta aatattcatt tgcaatatca acaaatgaat
    24961 taaatcttgt attgctcaac aacaattcgc ctaatgcgcg cataaaagcg aatagtacaa
    25021 ccccaataat catgtatgtg aataacagtg atggaccagt taaagatatt gtttgacctg
    25081 atcctaggaa taacccagtt ccaattgcgc ccccaattgc tatcaattgg atgtgtctgt
    25141 tattcaactc tctatgcaat tttcttgcca taattttcct cctcaggata aagcaaataa
    25201 aaaagttgtg tgtaagcgtt ttcttaatta taaatactaa agaaatccat ttaataaagc
    25261 aa
//
LOCUS       DAOBBH010000020        24957 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.020, whole genome shotgun sequence.
ACCESSION   DAOBBH010000020 DAOBBH010000000
VERSION     DAOBBH010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 24957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24957
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.020"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(79..1938)
                     /locus_tag="P6L01_000562"
     CDS             complement(79..1938)
                     /locus_tag="P6L01_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HDP3029653.1"
                     /translation="MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSK
                     KSNDDETSKDTTSKDTDKADNNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQT
                     NINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKND
                     TDTQSSKQDKADNQKAPKSNNTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSS
                     KDNQSMSDSALDSILDQYSEDAKKTQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKS
                     KPAQSFNNDVNQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRI
                     GQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNKL
                     YSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYA
                     TDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDSMPY
                     PHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQF
                     GADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK"
     gene            2149..5130
                     /locus_tag="P6L01_000563"
     CDS             2149..5130
                     /locus_tag="P6L01_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDP3029654.1"
                     /translation="MKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMW
                     DPYGNTGHIKVAVVNEDKGDTIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIK
                     MGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKA
                     NEQFNKTVTRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLN
                     NHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIE
                     KALNFAADDVPAQFPKINQGLNIASQGIDQANGQLNDAKGFVTQVRSRVGDYQDAIRR
                     AQDLNRRNQQQIPQNSAANNETSNSSPAAGNGVASTPPSAPSGDTAPNNNVTQNTAPN
                     SNNAPVSTTPQSTSGKKDGQSFADITTTQVSTANENTQNITDKDVKSMEAALTGSLLS
                     LSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEYTTQYNQQFID
                     TLKEIEKNENVDLSKEIDKVKAANNRINESLRLVNQLSNALKNGSSGTAEATKLLDQL
                     SKLDSSLSSFRDYVKKDLNSSLVSISQRIMDELNKGQTALSNVQSKLNTIDQVINSGQ
                     AILKNGKTRIDRLQTVLPSIEQQYISAVKNAQANFPKVKSDVAKAANFVRNDLPQLEQ
                     RLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFSQNKLPDVEKDLKK
                     ANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFPVKDYGSGMTPFYT
                     ALSVWVGALLMVSLLTVDNKHKSLESVLTTRQVFLGKAGFFIMLGMLQALIVSVGDLL
                     ILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAGGGGTFP
                     IQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGIGFFVVGLILK
                     PVTDPLMKRVSEKVDQSNVTE"
     gene            complement(5242..6180)
                     /gene="manA"
                     /locus_tag="P6L01_000564"
     CDS             complement(5242..6180)
                     /gene="manA"
                     /locus_tag="P6L01_000564"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDP3029655.1"
                     /translation="MALFLQPVFKDRIWGGHALKAFNYDIPNETTGECWAISAHPNGP
                     NTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDDYALK
                     HEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLIDMIDNHEFDSLFKRIPVKPGDFY
                     YVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNH
                     EPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLTV
                     NDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG"
     gene            complement(6194..8146)
                     /locus_tag="P6L01_000565"
     CDS             complement(6194..8146)
                     /locus_tag="P6L01_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HDP3029656.1"
                     /translation="MKIVAITSCPNGIAHTYMAQEKLEQVAKEMGVDIKVETQGGVGA
                     ENVLTTQDIEEADGVIIAADKQVDLSRFVGKRLINENVREGIHNPRGLIQRIINQDAP
                     IYQSETNYHSKDRGKSKSGIQMVYQHLMNGVSFMVPFIVVGGLLIAIALTLGGETTSK
                     GLVIPDDSFWKSIENIGSLAFKFMVPILAGYIAVSIADKPGLVPGMIGGAIAADGSFY
                     GSDAGAGFLGGIVAGFLAGYIAKWIKDIKVPKAMAPIMPIIIIPIISSVVVGLIFIFL
                     IGAPISNIFEALTTWLKSMQGANIIILAMIIGAMIAFDMGGPVNKVAFLFGSALIAEG
                     NYAVMGMVAVAVCTPPIGLGLATFVQKYKFNHSEREMGKASFTMGLFGITEGAIPFAA
                     QDPLRIIPANIIGAMIASVIAAIGGVGDRVAHGGPIVAVLGGIDHVLWFIFAVIVGSL
                     VTMTTVLLLKRNTPVIAVDAPAQHTQLHDTDITQHDTEVDNVDKTAKHSHLNKPSHVF
                     DQQTMIITDHDMSRNEAIDMLIHKLKICRYVEHTSHLKNAILEREMESTTAIGMNVAI
                     PHAKSDVVKQPIVAVMKNNHGVKWDSLDGSLPQLIFLIAVPNNSQDTHLKILQRLSKA
                     LMNDETRQSLINANSTTEIYNLLMKI"
     gene            complement(8222..10096)
                     /locus_tag="P6L01_000566"
     CDS             complement(8222..10096)
                     /locus_tag="P6L01_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDP3029657.1"
                     /translation="MLDRHLKLLQFFIKNPSKHISSNEIAEHVNVSNRTVRNDIHVIN
                     SNFMDDIIVSIKSKGYQLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQT
                     LQQLEQDYLLSKTVLNDYFVRIQQWCQKFNIVLTIKKKQGIVVDGTDNDITNAIIHLN
                     QLSSGHVHVEDLILNELPDSHQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKR
                     KQSLSELDTLNHDAKLISQSCIKQINERLGYDLNEKVINMFSFFIAYHFNKLDIGIER
                     IFIQSYIDRLIELMQQRIHVPFNEDSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIK
                     KLYPFVFNTLFEAIDKLAIDTDIEMSEDEIAFLTIHFQAAIERRTKTQLNVVIACYYG
                     LGVSNFLETKINNLSEELSVINTIKLESITHYHFDNVDLLITTHDIPKQTLNILPKHL
                     TTIKVAPLFSEDDRHKIRHVVKQKQNPVQAHHHMDTVNFLVVNTEQKSRHTVQIFEEA
                     QKILQAHHAIVEGYIESALEREKSSSTYIGNFMAIPHGDPEKVLQSHVLIFRTKDVFP
                     WRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDDDSVNHLCSLDDHSLKQQLFEYLQE
                     "
     gene            complement(10336..10794)
                     /locus_tag="P6L01_000567"
     CDS             complement(10336..10794)
                     /locus_tag="P6L01_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029658.1"
                     /translation="MKKLLITIVIIVLIGALGFAIYAAINHSTSSSNNQEQENKSTHK
                     KTETEENDQQDDSADKTDKNQNNGNNDTNDNQPSSPSNVPNNHSTVPSNTQPAQPKDS
                     NSNKNNTHGGGSQSSTNNQNNQQQNTPPANKSNNTNTPSKQTPQAQSPKN"
     gene            11052..11579
                     /gene="isaB"
                     /locus_tag="P6L01_000568"
     CDS             11052..11579
                     /gene="isaB"
                     /locus_tag="P6L01_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunodominant staphylococcal antigen IsaB"
                     /protein_id="HDP3029659.1"
                     /translation="MNKTSKVCVAATLALGTLIGVTVVENSAPTSKQAQAAITPYYTY
                     NGYIGNNANFILDKNFINAIKYDNVKFNGIKLAKTNTIKKVEKYDQTFKGVSAKGNEA
                     SQLQFVVKNNISLKDIQKAYGKDLKKENGKTKEADSGIFYYQNAKKTLGIWFVVDHNR
                     VVEVTVGHTPYKTSK"
     gene            12003..13532
                     /gene="aur"
                     /locus_tag="P6L01_000569"
     CDS             12003..13532
                     /gene="aur"
                     /locus_tag="P6L01_000569"
                     /EC_number="3.4.24.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloproteinase aureolysin"
                     /protein_id="HDP3029660.1"
                     /translation="MRKFSRYAFTSMAAVTLLSTLSPAALAIDSKNKPANSDIKFEVT
                     QKSDAVKALKELPKSENVKNIYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKV
                     HADKSGKVVLINGDTDAKKVKPTNKVTLSKDDAADKAFKAVKIDKHKAKNLKDKVIKE
                     NKVEIDGDSNKYVYNVELITVTPEISHWKVKIDAQTGEILEKMNLVKEAAETGKGKGV
                     LGDTKDININSIDGGFSLEDLTHQGKLSAFSFNDQTGQATLITNEDENFVKDEQRAGV
                     DANYYAKQTYDYYKDTFGRESYDNQGSPIVSLTHVNNYGGQDNRNNAAWIGDKMIYGD
                     GDGRTFTSLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFL
                     MGEDVYTPGKEGDALRSMSNPEQFGQPAHMKDYVFTEKDNGGVHTNSGIPNKAAYNVI
                     QAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGV
                     E"
     gene            13899..14348
                     /locus_tag="P6L01_000570"
     CDS             13899..14348
                     /locus_tag="P6L01_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDP3029661.1"
                     /translation="MKKSKRLEIVSTIVKKHKIYKKEQIISYIEEYFGVRYSATTIAK
                     DLKELNIYRVPIDCETWIYKAINNQTEQEMREKFRHYCEHEVLSSIINGSYIIVKTSP
                     GFAQGINYFIDQLNIEEILGTVSGNDTTLILTASNDMAEYVYAKLFK"
     gene            14351..14518
                     /locus_tag="P6L01_000571"
                     /pseudo
     CDS             14351..14518
                     /locus_tag="P6L01_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501420.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14695..15930
                     /gene="arcA"
                     /locus_tag="P6L01_000572"
     CDS             14695..15930
                     /gene="arcA"
                     /locus_tag="P6L01_000572"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDP3029662.1"
                     /translation="MTDGPIKVNSEIGALKTVLLKRPGKELENLVPDYLDGLLFDDIP
                     YLEVAQKEHDHFAQVLREEGVEVLYLEKLAAESIENPQVRSEFIDDVLAESKKTILGH
                     EEEIKTLFATLSNQELVDKIMSGVRKEEINPKCTHLVEYMDDKYPFYLDPMPNLYFTR
                     DPQASIGHGITINRMFWRARRRESIFIQYIVKHHPRFKDANIPIWLDRDCPFNIEGGD
                     ELVLSKDVLAIGVSERTSAQAIEKLARRIFENPQATFKKVVAIEIPTSRTFMHLDTVF
                     TMIDYDKFTMHSAILKAEGNMNIFIIEYDDVNKDIAIKQSSHLKDTLEDVLGIDDIQF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNDLLRQKGIKVIEISGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            15963..16973
                     /gene="argF"
                     /locus_tag="P6L01_000573"
     CDS             15963..16973
                     /gene="argF"
                     /locus_tag="P6L01_000573"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDP3029663.1"
                     /translation="MTEIQKPYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGI
                     KHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKV
                     LGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEG
                     INLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKEAYVDIAKEKASQYGGSIM
                     ITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQMFDLTGKDSTIFLH
                     CLPAFHDTNTLYGQEIYEKYGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATL
                     GS"
     gene            17064..18494
                     /gene="arcD"
                     /locus_tag="P6L01_000574"
     CDS             17064..18494
                     /gene="arcD"
                     /locus_tag="P6L01_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDP3029664.1"
                     /translation="MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAII
                     IGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLG
                     NVAYATLLMSAVGNFFPIFKGGNTLPSIIVASLLLWGVHFLILKGVETAAFINSIVTV
                     AKLIPILLVIICMIIAFNFDTFKTGFFGMTSEGVLPFSWASTMSQVKSTMLVTVWVFI
                     GIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMA
                     QVLATIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNG
                     APVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQ
                     QATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYSIVQKNNQTRLIKSD
                     YILFMIIIVLAVIGLIKLLMGTINVF"
     gene            18511..19452
                     /gene="arcC"
                     /locus_tag="P6L01_000575"
     CDS             18511..19452
                     /gene="arcC"
                     /locus_tag="P6L01_000575"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDP3029665.1"
                     /translation="MKEKIVIALGGNAIQTKEATAEAQQTAIRRAMQNLKPLFDSPAR
                     IVISHGNGPQIGSLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMN
                     SDRTVGTIVTRVEVDKDDPRFNNPTKPIGPFYTKEEVEELQKEQPDSVFKEDAGRGYR
                     KVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASE
                     KLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFAEGSMLPKI
                     EAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM"
     gene            19551..20255
                     /locus_tag="P6L01_000576"
     CDS             19551..20255
                     /locus_tag="P6L01_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDP3029666.1"
                     /translation="MTENFILGRNNKLEHELKALADYINIPYSILQPYQSECFVRHYT
                     KGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNKEQVLFPISNLFHPKEVNE
                     LCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNEQQHMNYNMALTSKFAKDR
                     IIKLLCHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRETAGHIIHELKDEKLVVKD
                     HKNWLVSKHLFNDVCV"
     gene            20605..23148
                     /gene="clfB"
                     /locus_tag="P6L01_000577"
     CDS             20605..23148
                     /gene="clfB"
                     /locus_tag="P6L01_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfB"
                     /protein_id="HDP3029667.1"
                     /translation="MKKRIDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQA
                     SEQSNDTTQSSKNNASADSEKNNMIETPQLNTTANDTSDISANTNSANVDSTTKPMST
                     QTSNTTTTEPASTNETPQPTAIKNQATAAKMQDQTVPQEANSQVDNKTTNDANSIATN
                     SELKNSQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNDK
                     VTASNFKLEKTTFDPNQSGNTFMAANFTVTDKVKSGDYFTAKLPDSLTGNGDVDYSNS
                     NNTMPIADIKSTNGDVVAKATYDILTKTYTFVFTDYVNNKENINGQFSLPLFTDRAKA
                     PKSGTYDANINIADEMFNNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQ
                     TVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDTSKLSDSYYADP
                     NDSNLKEVTDQFKNRIYYEHPNVASIKFGDITKTYVVLVEGHYDNTGKNLKTQVIQEN
                     VDPVTNRDYSIFGWNNENVVRYGGGSADGDSAVNPKDPTPGPPVDPEPSPDPEPEPTP
                     DPEPSPDPEPEPSPDPDPDSDSDSDSGSDSDSGSDSDSESDSDSDSDSDSDSDSDSES
                     DSDSESDSDSDSDSDSDSDSDSESDSDSDSDSDSDSDSDSESDSDSESDSESDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSRVTPPNNEQKAPSNPKGEVNHSNK
                     VSKQHKTDALPETGDKSENTNATLFGAMMALLGSLLLFRKRKQDHKEKA"
     gene            23264..24025
                     /locus_tag="P6L01_000578"
     CDS             23264..24025
                     /locus_tag="P6L01_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDP3029668.1"
                     /translation="MAYISLNYHSPTIGMHQNLTVILPEDQSFFNNDITAKPLKTLML
                     LHGLSSDETTYMRYTSIERYANEHKLAVIMPNVDHSAYANMAYGHSYYDYILEVYDYV
                     HQIFPLSKKRDDNFIAGHSMGGYGTIKFALTQGDKFAKAVPLSAVFEAQNLMDLEWND
                     FSKEAIIGNLSSVKGTEHDPYYLLDKAVAEDKQIPKLLIMCGKQDFLYQDNLDFIDYL
                     SRKNVPYQFEDGPGDHDYAYWDQAIKRAITWMVND"
     gene            24270..24725
                     /locus_tag="P6L01_000579"
     CDS             24270..24725
                     /locus_tag="P6L01_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDP3029669.1"
                     /translation="MSTNKNDYEHMLFYFAYKTFITTADEIIEKYGMSRQHHRFLFFI
                     NKLPGITIKSLLEILEISKQGSHATLQKLKEQGLIIEKVLETDRRVKKLYSTDKGDQL
                     IAELNKAQDELLQNIYQQVGSDWYDVMEALAKGRPGFDFIKHLKDEKES"
     gene            complement(24828..24938)
                     /locus_tag="P6L01_000580"
                     /pseudo
     CDS             complement(24828..24938)
                     /locus_tag="P6L01_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501411.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         8738 a         3757 c         4601 g         7861 t
ORIGIN      
        1 aatcggactt atgaacagaa atatgtttaa gtccgaactc cttgtttata cttataaatt
       61 ttgcgggttt aataatactt atttacctgt aatatatgat aattcttcag cggcagctgc
      121 attgatagtt ctatgagaaa tgatacctaa tcctttaaca ttggattctg aaataacgat
      181 agaaccatca ctgttaactt tttcaacaaa tgctacatga ccgtaatgtt gatctgcacc
      241 aaattgtcca gcctcaaata caacagcagc atgacgtttt ggtgtatgac ttacttgata
      301 atcacggtat tgagctcgat tattccaatt atgtgcatca cctaaatcac ctgagataga
      361 tgtaccaaat tgtttcatac ggttatatac gtaccaagta cattggccat gtggatatgg
      421 catactatca gatacctcgc ggaaaggttt gaattcatct gatgaatcat cataatcctt
      481 gatagaacgt tcatatttat ctaaatctgg catgcgttca tcgtcaaact gagttaattg
      541 atagtgttta ataatactgt ttaatttctt agcatagttt ggatctgtag catatgtttt
      601 agataagtgt gatgttgcat ctttataaga atcggcttcc gatttccatg ttggtttata
      661 aattgttcga ttgccatcaa taccattttt aataaggtca gagtaatctt ttagtgattc
      721 tttcgtgctt ggatattttc ggaatccagc attaatacta tacaatttat taccatcagc
      781 ttctaatgtg ttaaaaggaa cagaattccc ttcaaaagca cctttgatac cgaataaatt
      841 atggtttggt gacttagcta aagcactacg acctgagtca gattctaaga ttgcttgggc
      901 aatcatgaca gacgcataaa tatcgttatc ttgaccaatg cgatgtgcat ctttagcaat
      961 tgatttgaca aattgacgtg tatcttttga gtcaacaacg ttaaattgtc cgctatcatc
     1021 attgttagat atactaggat ctgtttcgaa taatgatgtt gcacgtgtat ccttttgatt
     1081 aacatcgtta ttgaatgatt gagcaggttt agatttatgt ttcaattcat cttgtgttgg
     1141 taactgtgga ttctttgtat tagatttttc atttttgtct tttttagatt gagatgcata
     1201 atctttttgt gttttctttg catcttcact gtattgatcc aaaatagagt ctaaagccga
     1261 atctgacatt gattgattat ctttcgatga agatttttga tttgctttat cgtcacttgc
     1321 tggttgacta tttgattgat taggttgtgt tggctttggc gaatttggtt gcttattaga
     1381 tgtacttggt tttgtattgt ttgatttagg tgctttttga ttgtctgctt tatcttgttt
     1441 agatgattgc gtatcagtgt catttttgat gctattgtca ctgtttttat tcgaatcatt
     1501 tgttgacttt tcgccattac gaggttgttc gtaatcagaa atatccgaat ttaaattgaa
     1561 taagttttgg attaaagttg ttaatgagta attatcatcg tatttatttt tggttagcaa
     1621 ttggtttata ttggtttgtg gtaaattctt ataaataaaa tcaatgatat tgttagagtc
     1681 tgaagtgctg tcgtctatag ttttaaattt tttgtcgtta ttgtcttggt tacttgtatt
     1741 attgttgtct gctttatcag tatctttact tgtagtatcc ttagaagttt catcgtcatt
     1801 agattttttt gaatcatgag atgttgtctt agctgtagta tctttttgag gtgtatcagc
     1861 ataagcggta ggtgaaacta aagtaggtaa tacgagcgta gttgatagca aataaattaa
     1921 aattttattt ttaggcatat ttcgtattct cccttgaaaa atataataat taagtgtgat
     1981 aataaactat gatttgttat aatttatcgt atgctgaaaa tagttgatag gtatcaatcg
     2041 actaaatatc ttccagtaaa ttgattatac taattcacaa cgcaaaaata aattaattta
     2101 caaaaaatat ataaaaaata tgaataattc ctacatagga gtgtgacaat gaagaacgca
     2161 tttaaattat ttaaaatgga tctgaagaaa gtagctaaga cgccagctgt gtggattatc
     2221 ttagcaggct tagctatttt gccatcgttc tacgcttggt ttaacttatg ggcaatgtgg
     2281 gatccatatg gcaacacggg acacatcaag gtcgcagtcg ttaatgaaga taaaggcgac
     2341 acaatcagag ggaaaaaagt taatgtcggt aatacgatgg ttaatacact caagaaaaat
     2401 aaaagttttg attggcagtt tgtaagtaga gagaaagctg atcatgagat aaaaatgggt
     2461 aaatattttg caggtattta catcccatct aagtttacac atgaaattac cgggacacta
     2521 cgtaagcagc ctcaaaaagc agatgtagaa tttaaggtga atcagaagat taacgctgtt
     2581 gcgtctaagc taacagatac tggttcgtca gttgtcgttg aaaaagcgaa tgaacaattt
     2641 aataaaacag taactcgagc attattagaa gaagctaaca aagcaggttt aactattgaa
     2701 gaaaatgtgc cgacaattaa caagataaaa aatgcggtat attcagcaga taaagcttta
     2761 cctaagatta atgactttgc gaataaaatt gtgtatttga ataaccacca agcggattta
     2821 gataaatatg ccaatgattt tagaaaacta ggaaattata aaggtgatat tttagatgct
     2881 cagaaaaaat taaacgaagt caatggtgct attccgcaac ttaatgaaaa ggctaagttg
     2941 atattagctt taaataatta tatgccgaaa attgaaaaag cgttaaattt tgcagctgat
     3001 gacgtgccag cgcagttccc taaaattaat caaggactta acattgcgag tcaagggatt
     3061 gatcaagcta atggacagtt aaatgatgcc aaaggcttcg tcacacaagt tagaagtaga
     3121 gtcggtgatt atcaagatgc aattcgacgc gcgcaagatt taaatcgaag aaaccagcaa
     3181 cagattcctc aaaatagcgc ggcgaacaac gaaacatcaa atagttcacc tgcagctggt
     3241 aatggtgtag catcaacgcc accaagtgca ccaagtggcg atactgcacc aaataataat
     3301 gttacgcaaa ataccgcacc aaatagtaat aatgcgcctg tatcgactac accacaaagt
     3361 acaagcggga aaaaagatgg tcaaagtttt gcagatataa caacaacaca agtcagcaca
     3421 gctaacgaga acacacaaaa cattacagat aaagatgtta aatcaatgga agcggcatta
     3481 acgggctctt tattatcatt atcaaataat ttagataccc aagcgaaagc cgcacaaaaa
     3541 gatagtcagg cattacgtaa tatttcgtat ggcattttag catcggacaa gccttctgat
     3601 tttagagagt ctttagataa tgttaagtcc ggtttagaat acacaacgca atataatcaa
     3661 caatttatcg atacattaaa agagattgag aagaatgaaa atgttgattt atcaaaagaa
     3721 attgataagg taaaagcagc taataatcga attaatgaat cattaaggtt agttaatcaa
     3781 ttaagcaatg cattaaagaa tggtagttca ggaactgctg aagctactaa attactagat
     3841 caactttcaa aactagattc atcattatca tcatttagag attatgttaa aaaagatctt
     3901 aacagctctt tagtatcaat atcacaacgt attatggatg aattgaacaa agggcaaact
     3961 gcattatcca atgttcagtc taaattaaat acaattgatc aagtcattaa cagtggacaa
     4021 gctattttaa aaaatggtaa aacacgtatt gatcgcttac aaacagtatt accaagtatt
     4081 gaacaacaat acattagtgc tgttaaaaat gctcaagcaa acttcccgaa agtgaaaagt
     4141 gatgtagcta aagctgctaa ctttgtgcgc aatgacttac cacagttgga acagcgatta
     4201 actaatgcga cagcaagtgt gaataaaaat ttaccaacgt tattaaatgg ttatgatcaa
     4261 gcggtaggat tactaaataa aaatcagcca caagcgaaaa aggctttatc agatttagct
     4321 gatttttctc aaaataaatt gcctgatgtt gaaaaagatt tgaaaaaagc gaataaaatt
     4381 ttcaagaaat tagacaaaga tgatgcagtc gacaaattaa tcgacacact taagaatgat
     4441 ttgaaaaagc aagcgggtat tattgcaaat cctattaata agaagactgt tgatgttttc
     4501 ccagttaagg attatggttc aggtatgaca ccattctata ctgcactgtc agtatgggta
     4561 ggtgcactct tgatggtaag tttattaacg gttgataata aacataagag tctagagtca
     4621 gtcttaacga caagacaagt gttcttaggt aaggcaggat tctttataat gcttggtatg
     4681 ttgcaagcac tcattgtatc ggttggagat ttgttaatcc taaaagcagg agttgagtca
     4741 cctgtattat ttgtacttat aacgattttc tgttcgatta ttttcaactc aatcgtatat
     4801 acgtgcgtat cattacttgg taacccaggt aaagcaattg caatcgtatt gcttgtatta
     4861 caaattgcag gtggtggagg aacattccca attcaaacta cgccacaatt tttccaaaac
     4921 atttcgccat acttaccatt tacgtatgca attgattcat tacgtgaaac agtaggcggt
     4981 attgttccgg aaatcctaat tacaaaatta attatattaa cgttatttgg cataggattc
     5041 ttcgttgtag gtttaatttt aaaacctgta acagatccat tgatgaagcg cgtatctgaa
     5101 aaagttgacc aaagtaacgt tacagaataa aaattaaatc cacacattag ggttatagct
     5161 ccttaatgtg tggattttta tgtttttaga cagaagagat agtaatttct gtcttttatg
     5221 ggacggttgt tatcattgct attatccagg atgacttact atagcactaa tattaccgac
     5281 aaagtgaata tcctcgtctt ccgtagttaa aataaagcta gaaccttttt ggatgtcata
     5341 gtgcttatcg tttactgtta aagtaccagt accatcgata atagtaacta agcaataagc
     5401 atgtggttta ttgaatttta aatctccatg aatatcccat ttatatactg caaaatattg
     5461 attatctaca aattgagtta cagtgtgtgt gtcgatgtga gttgttatag gagtagtatt
     5521 tggttcatga ttgcctaatt caatcacatc tttactttgc tctaagtgca aatcacgcaa
     5581 ttgaccattt tgatctcgtc tatcatagtc ataaatacgg tatgtcgtat cggatgattg
     5641 ttgtgtctct aaaattaaaa tacccgaacc aatggcatgg acagtgccag caggaacata
     5701 ataaaagtca ccgggcttaa caggtatacg tttgaaaaga ctgtcaaatt catgattatc
     5761 aatcatgtcg attaacgtct gtttattatg tgcatgtacg ccatatataa tttcagcacc
     5821 tggggctgca tctaaaatat accaacattc tgttttacct agttcgcctt cgtgttttaa
     5881 agcgtagtca tcatctggat gaacttgaac agataattta tcattggcat ctaatacttt
     5941 agttagcaga gggaaactat ctcgtgaatc attatcgaat aattcacgat gttgtgacca
     6001 aagttgatct agggtcatat ccttgtatgg accattgata attgtattag gaccatttgg
     6061 atgtgcagaa attgcccagc attcaccagt tgtttcatta gggatatcat agttaaatgc
     6121 ttttaatgca tgaccgcccc aaattctgtc tttaaaaacg ggttgtaaaa ataatgccat
     6181 agttaaaact cctctatatt ttcattaata agttataaat ttctgtagta ctgtttgcat
     6241 taattagtga ttggcgtgtc tcatcattca ttaacgcttt agataagcgc tgaagtattt
     6301 ttaaatgtgt atcctgactg ttgtttggta cggcaattaa gaatatcaat tgaggtagac
     6361 taccatctag actgtcccat ttaacaccat gattattttt cataacagct acaatcggtt
     6421 gttttacaac atcagacttt gcatgtggaa tggccacgtt catgccaata gctgtcgtag
     6481 actccatttc acgttctagt attgcatttt ttaaatgcga tgtgtgctct acataacggc
     6541 aaattttaag tttatgaatc aacatatcaa ttgcttcgtt tcgagacatg tcgtgatcag
     6601 taattatcat agtttgttga tcaaaaacat gagaaggttt attgagatgt gaatgtttcg
     6661 cggttttatc tacattgtca acctctgtat catgttgtgt aatatctgta tcatgaagtt
     6721 gcgtgtgttg cgctggtgca tctactgcta taactggtgt attgcgtttt aataatagta
     6781 cagtagtcat tgtgacaaga ctacctacta tcactgcaaa gataaaccat aatacatgat
     6841 caataccacc taatacagcc acgattggac ctccatgtgc gactctatcg ccgacaccac
     6901 caatggctgc aatgactgat gcaatcattg caccaatgat gtttgcaggt ataatgcgca
     6961 atggatcttg ggctgcgaaa ggaatagcac cttcagtaat accaaatagt cccatagtga
     7021 aggaagcctt acccatttct ctttcggaat gattgaattt atacttttga acaaacgttg
     7081 ctaaacctaa accgattggt ggtgtacata cagcaactgc gaccataccc ataacggcgt
     7141 aattaccttc agcaataagt gctgagccaa ataaaaatgc taccttgttt actggaccgc
     7201 ccatatcgaa ggcgatcatc gcacctataa tcatcgcaag tataataata ttagcacctt
     7261 gcatactttt taaccaggtt gttaatgcct caaaaatatt agaaattggt gcaccgatta
     7321 aaaatataaa tatcaatcct acaacgaccg atgaaataat gggaataata atgataggca
     7381 taattggtgc cattgctttt ggaactttaa tatctttaat ccactttgcg atataacctg
     7441 ctaagaaacc agcaacaata ccacctaaaa atcctgcgcc tgcatcactg ccataaaaac
     7501 taccgtcagc agcgatagcg ccgccaatca taccaggaac aagaccgggc ttgtcagcga
     7561 tactaacagc gatatatcca gctagtattg gaaccataaa tttaaaggct aaactaccaa
     7621 tgttttcaat ggatttccaa aatgaatcat ctgggatgac taatcctttt gatgtcgttt
     7681 caccgcctag agtcagcgcg atggcgataa ggagtccacc aactacgata aaaggaacca
     7741 taaacgatac accgttcatt aaatgttgat acaccatttg aataccactt ttagacttac
     7801 cgcgatcttt cgaatgataa tttgtttcag attgataaat aggcgcatct tgattaatga
     7861 tacgttgaat tagacctctc ggattatgaa tcccttcgcg aacattttca ttaatcaacc
     7921 gtttaccaac aaatcgggat agatcaactt gtttatcagc tgcaattatg acaccgtcag
     7981 cttcttcgat gtcttgcgta gttaaaacat tttcagcacc aacaccgccc tgtgtctcta
     8041 ctttaatatc cacacccatt tcttttgcta cctgctcaag cttttcttga gccatatatg
     8101 tatgtgcaat gccatttggg catgaggtaa tagctacaat tttcataaaa tcatctcctt
     8161 ttctatattg taagcgtatt ctcgatacta aaaaaagaat aattaccgtt actagtggca
     8221 attattcttg taagtattca aataactgtt gctttaaact atgatcatct aaactacata
     8281 aatggttcac tgaatcatca tccaagttag caattaattg catcatttgt tttgtaaaag
     8341 ctttgtcttt atgcgaaatc gctaagaaaa agacaagttt gacatcgtgt tgtcgccaag
     8401 gaaaaacatc ttttgtgcga aaaataagca catgtgattg taaaactttt tcaggatctc
     8461 catgaggaat cgccataaaa ttacctatgt atgtagaaga tgatttctca cgctctaaag
     8521 ctgattcgat atatccttct acaatcgcat gatgtgcttg taatattttt tgagcttctt
     8581 caaaaatttg cacagtatgc cgtgattttt gttcagtatt tacgacaagg aaattgacag
     8641 tgtccatatg atgatgtgct tgaaccggat tttgcttttg cttcacaacg tgtctgattt
     8701 tgtgacgatc atcttcagaa aataatggtg caaccttgat agtcgtcagg tgcttaggaa
     8761 gtatgtttag cgtttgtttt ggaatatcat gggtcgttat taataaatct acattgtcaa
     8821 agtgatagtg tgttatactt tctagtttaa tcgtatttat tactgacaac tcttcggata
     8881 agttatttat tttagtttct aaaaaattcg acacacctag accataataa caagcaatga
     8941 ctacatttaa ttgtgttttg gtacgacgct cgatggcagc ttgaaaatga attgttaaaa
     9001 atgcaatttc atcttcgctc atctctatat cagtatcaat tgctaattta tcaatcgctt
     9061 caaaaagtgt gttaaacaca aagggataga gttttttaat ctctataact aaaggattgt
     9121 ttaaataaat gttttgagtg atacgtaaat atgctttact aaaatgatta tataaatttt
     9181 gttgtaaaat cgaatcttca ttgaaaggta catgaatacg ttgctgcatc aattcgatta
     9241 agcgatcaat ataactttgt ataaatatac gttctatacc aatatcgagt ttattaaaat
     9301 gataagcaat aaagaatgaa aacatattga ttactttttc gttcaagtca taacctaatc
     9361 tttcgttgat ttgcttaatg caagattgag atatcaattt tgcatcatgg tttaacgtgt
     9421 ctagctcact taaagattgc ttacgtttaa tgattaaaat gagatgaatt aaaagctgtt
     9481 gtatttgaat atcagttgtt tcaatactat gttgttgaag tgtctcttgt ataatatgcg
     9541 aaatcatcct ttggtgtgaa tcaggtaatt catttaaaat taggtcttca acatgtacat
     9601 gccctgatga taattgattt aaatggatga tggcattagt gatatcatta tctgttccat
     9661 cgaccacaat accttgtttt ttcttgattg ttaaaacgat attaaatttt tgacaccatt
     9721 gttgaattcg aacgaaatag tcgttgagca cagttttaga taatagataa tcttgctcta
     9781 gttgttgcaa tgtctgcgat ttattgtgca ttagcagttg atacgccata gataataata
     9841 gtttttcttt atatgattga atatgcgtgt aacgttcagt tatcgtttct aaggtatact
     9901 gcgatgtatt taattgatat cctttagatt taatacttac gatgatatca tccataaaat
     9961 tgctattgat aacatgaata tcattgcgaa ctgtgcggtt tgaaacattg acatgttcag
    10021 caatttcatt agaactaatg tgctttgatg gatttttaat aaaaaactgg agtagtttta
    10081 agtgtctatc aagcatttaa catgtacctc ctttctaaat ttttcgtgta agcgttttta
    10141 aaggtgtatt aatattatta aacatgagag cttatacata cgtcaatgac attaaagcga
    10201 acttttatat gattttaaca gagtgcgaat tatgcaaata aagaacagca gtaagatatt
    10261 tcaaatagaa aaatatctca ctgctgtttt tctgaattta tgcatctggt acttgtggac
    10321 gtatcaggca aatgattaat ttttaggtga ttgtgcttga ggtgtttgtt tagaaggcgt
    10381 attggtatta tttgatttat tagcaggtgg tgtgttctgt tgttgattat tttggttgtt
    10441 tgttgacgat tgactgccac caccgtgagt attattttta tttgaatttg agtcttttgg
    10501 ctgagctggt tgcgtattcg atggtactgt tgaatggttg ttaggaacat ttgacggtga
    10561 agatggttga ttgtcattag tatcgttatt gccattattt tgatttttat ctgttttgtc
    10621 tgcactatca tcttgttgat cattttcttc ggtttctgtt tttttatgcg tagatttatt
    10681 ttcttgttct tggttattac ttgatgatgt agaatgattt attgcagcat aaattgcgaa
    10741 cccaagtgct ccgattaata cgataattac gatggtaata agaagttttt tcaaatataa
    10801 cgcctccatg ttaatgaaaa gtagttgata cgtgaagcta aataatttat ttcaatattg
    10861 ccataatcat tttaaaaaat aaatattaga aaagtaggcg atattaatat tttcattgtt
    10921 ttttgtgtac ttcaattagt ataacaaaac tgttatgtat ttataaatat aatcattagt
    10981 atttttatgg ctgaaaaagt tataataaaa gtgtaaggaa taaaatattt gtatggaaaa
    11041 gagagataat tatgaataaa accagtaaag tttgtgtagc agcaacatta gcattgggca
    11101 cactgattgg agtaacagtt gttgaaaatt cggcgccaac aagtaaacag gcacaggcag
    11161 caataacccc atattatact tataatggtt atattggtaa taatgctaat tttattctgg
    11221 ataagaattt tattaacgcg attaagtatg ataatgtgaa attcaatggt attaaattag
    11281 ctaaaacgaa tacgataaaa aaagtagaga aatatgatca aacttttaaa ggtgtttctg
    11341 caaaaggaaa cgaagcaagt caattgcaat ttgtagttaa aaataatatt tcattaaaag
    11401 atatccaaaa agcttatggc aaggacttga aaaaagaaaa tggtaaaaca aaggaagctg
    11461 atagcggtat tttttactat caaaatgcta aaaagacatt aggcatctgg tttgtcgttg
    11521 atcataatag agttgtcgaa gtaacagttg gacatacacc atacaaaaca agtaaataaa
    11581 ataatggcat attaaggcta gagtgtgagg agagataccg cattctagtc ttttttatta
    11641 aataataacg attattgcgt cttacatagt tgtttgaaat tgataaaaat gtttatttga
    11701 tcatgattat ttaaatagtt atctcacata tttcaagcat tgattaagta tatagagcac
    11761 tattttgtat ttgttaatat tttcacaaaa ataaagcctt gataaatttt aaaatataat
    11821 taagctcaat ttttaaaatt ttatttagct acagataaca tttttaaaaa agaaaagaat
    11881 caataaataa aatcaacgaa caaaaagtat cgaaataaat agaaataatc atttactttt
    11941 ctgaaaaatt aaattaatat tttatttata agctgttttt aagatttcag gaggaatgaa
    12001 atgtgaggaa attttcaaga tatgcattta caagtatggc agcagtaaca ttgttgagca
    12061 ctttatcacc agcagcatta gcgattgatt caaaaaataa accagctaat tctgatatta
    12121 aatttgaggt gactcaaaag agtgatgcgg tcaaagcatt aaaagaattg cctaaatccg
    12181 aaaatgtaaa aaatatttat caagattacg ctgttactga tgtaaaaact gataaaaaag
    12241 gatttacgca ttatacattg caaccgagtg ttgatggtgt tcatgcacct gacaaagaag
    12301 tgaaagtaca cgcagacaaa tcaggaaaag tcgttttaat caatggggat actgatgcga
    12361 agaaagtaaa gccaacgaat aaagtgacat taagtaaaga tgacgcagcc gacaaagcat
    12421 ttaaagcagt taagattgat aagcataaag cgaaaaatct taaagataaa gtcattaaag
    12481 aaaacaaagt tgaaatcgat ggtgacagta ataaatacgt ttataatgtt gagttaatta
    12541 cagtgacacc agaaatttca cattggaaag ttaaaattga tgctcaaact ggcgaaattt
    12601 tagaaaaaat gaacttagtt aaagaagctg cagaaactgg taaaggaaaa ggtgtacttg
    12661 gcgatacaaa agatatcaat atcaatagta ttgacggtgg atttagccta gaagatttaa
    12721 cgcatcaagg taaattatca gcatttagct ttaatgatca aacaggtcaa gcaacattga
    12781 ttactaatga agatgaaaac ttcgtaaaag atgagcaacg tgctggcgta gatgcaaatt
    12841 attacgctaa acaaacatat gattattaca aagacacatt tggtcgtgaa tcatatgaca
    12901 accaaggtag tccaattgtt tcattaacgc atgttaataa ctacggtggt caagataaca
    12961 gaaataatgc cgcatggatt ggtgacaaaa tgatctatgg tgatggtgat ggtcgcacat
    13021 tcacaagttt atcgggtgca aatgacgtag tggcacacga attaacacac ggtgtgacac
    13081 aagagactgc gaacttagaa tataaggacc agtcaggcgc tctaaatgaa agcttttcag
    13141 atgtttttgg atactttgta gatgacgagg atttcttaat gggtgaagat gtctacacac
    13201 ctggaaaaga gggggacgct ttacgcagca tgtcaaaccc agaacaattt ggtcaaccag
    13261 ctcatatgaa agactatgta ttcactgaaa aagataatgg tggcgtacat acgaattctg
    13321 gaattccaaa taaagcagct tataacgtga ttcaagcaat agggaaatct aaatcagaac
    13381 aaatttacta ccgagcatta acggaatact taacaagtaa ttcaaacttc aaagattgta
    13441 aagatgcatt ataccaagcg gctaaagatt tatatgacga gcaaacagct gaacaggtgt
    13501 atgaagcatg gaatgaagta ggcgtggagt aaaaatatat aaacaagaag aagtaatgtt
    13561 aaacacttat aaataattaa attttaaata cagcttatat atggggtata cctacattga
    13621 taattgactt tgtaggtata ccctctcttt gtaattagat gagaggagtg tgagggttgt
    13681 ctgccgaaag actactcgac agtctaaaat cattacaagt agtagatatg tgataattaa
    13741 atgctgactt agaatacaaa attcatttta aaagttgtca caaaaaattt acatgtattt
    13801 ttattatctt ttgcaaaaca aagtgttaaa ttataaatga aacatgcatg aatttatttt
    13861 ttaatacaag aaacgtaact accaaaggag tttacaatat gaagaaaagt aaacgattag
    13921 aaattgtttc tacaatagtt aaaaagcata agatttataa aaaagaacaa atcatttcat
    13981 atattgaaga atattttggt gtaagatata gtgcaacaac aattgctaaa gacttgaaag
    14041 aacttaatat atatcgtgtc cctatcgatt gtgaaacatg gatttataaa gctattaata
    14101 atcaaacaga acaagagatg agagaaaagt ttagacacta ttgtgaacat gaagttctaa
    14161 gttcaatcat caatggttca tacattatcg ttaaaacctc acctggtttc gcccaaggca
    14221 taaactattt tatcgatcag ctaaatatag aagagatatt aggtacggtg agtggaaatg
    14281 acactacatt aatcttaact gcctcaaatg atatggcaga atacgtatat gcaaaattat
    14341 ttaaatagac atgtatcaaa tgaataataa aaattagttt cgtatcacgt gtacacaagt
    14401 tagttaccaa atattaactt gtgtacgcgt ttttttatgg aaagaaagaa ttcatagtca
    14461 ttcatttgac tgtataaaaa actttataca acgtgttttt gtgggtcttt ttgaataaaa
    14521 aatgtatatt ttgacccaaa acacctttat ttatgtataa aaatccatta ttatgtattg
    14581 tataacaaaa agatatgaaa ttttcgactt tctttatgtg aatataatca catgtaagcg
    14641 tttgaagatt gtctatactc taaatgaatt caaagataaa aggaggaaat agacatgaca
    14701 gatggtccaa ttaaagtaaa tagcgaaatt ggagctttaa aaactgtgtt acttaagcgt
    14761 cctggaaaag aattagaaaa tttagtacct gattatttag atggattact atttgatgat
    14821 attccatatt tagaagtagc tcaaaaagag catgaccatt ttgcgcaggt gctaagagaa
    14881 gagggtgttg aagtacttta ccttgagaag ttagcagctg aaagtattga aaatcctcaa
    14941 gtaagaagtg aatttattga tgatgtatta gcagagtcta aaaaaacaat attaggtcat
    15001 gaagaagaaa ttaagacatt atttgcgaca ctttctaatc aagaacttgt agataaaata
    15061 atgtcagggg tacgtaagga agaaattaat ccgaaatgta cacatctagt agagtacatg
    15121 gatgataagt atccattcta tttagatcca atgccaaacc tttattttac tagagatcca
    15181 caagcctcaa taggacacgg tataacaatc aatcggatgt tctggagagc acgacgacga
    15241 gaatcaatat ttattcaata tattgtaaag catcatccta gatttaaaga tgcgaatatt
    15301 ccaatctggt tagatcgaga ttgcccattc aatattgaag gcggcgatga acttgtttta
    15361 tctaaagatg tcttggctat aggcgtttca gaacgtacat ctgcacaagc tattgaaaag
    15421 ttagcgcgac gtatttttga aaatccgcag gcgacgttta aaaaagtagt agcaattgaa
    15481 attccaacta gtcgaacttt tatgcactta gatacagtat ttacaatgat agattatgac
    15541 aaatttacaa tgcattcagc cattttaaag gcagaaggca atatgaatat atttattatt
    15601 gaatatgatg acgtaaataa agatattgcc atcaaacaat ctagtcattt aaaagatact
    15661 ttagaagacg tactaggtat agatgatatc caattcattc caacaggaaa tggtgatgtc
    15721 attgatggtg ctagagagca atggaatgat ggctcaaata cattatgtat aagaccaggc
    15781 gttgtagtga cttacgatag aaactatgta tcgaatgatt tattgagaca aaaaggcata
    15841 aaagtcattg aaatatctgg tagcgagctg gtacgtggac gtgggggccc tagatgtatg
    15901 agtcaaccac tattcagaga agacatttaa cgtaataaat tatagaagcg agggtgaaaa
    15961 taatgacaga aattcaaaaa ccgtatgatt taaaaggcag atcattatta aaagaaagtg
    16021 attttaccaa agcagaattc gaaggactta ttgattttgc aattacatta aaagagtata
    16081 agaaaaacgg tattaagcat cactacttat ctggaaaaaa tattgcacta ctattcgaaa
    16141 agaattcgac gagaacgcgt gcagcgttta cagttgcgtc tattgattta ggtgcgcatc
    16201 cagaattttt agggaaaaat gatattcaat taggcaaaaa agaatctgta gaggatactg
    16261 cgaaagtatt aggtagaatg ttcgatggta ttgaattccg tggtttttca caacaagctg
    16321 ttgaagattt agcgaagttc tctggtgtac cggtgtggaa tggattaaca gacgattggc
    16381 atcctacaca aatgttagct gattttatga caataaaaga gaattttgga tatctagaag
    16441 gaataaactt aacttacgtt ggagatggac gtaataatat tgcgcattca ttaatggtag
    16501 caggtgctat gttaggtgtt aatgtaagaa tttgtacacc taaatcatta aatccaaaag
    16561 aggcatatgt tgatattgca aaagaaaaag cgagtcaata tggtggttca atcatgatta
    16621 cggataatat tgcagaagca gttgaaaata cagatgctat atatacagat gtttgggtat
    16681 cgatgggtga agaaagtgaa tttgaacaac gtattaattt attaaaagac tatcaagtga
    16741 atcaacagat gtttgattta acaggtaaag attcaacgat attcttacat tgtttaccag
    16801 cattccatga tacaaataca ctttatggac aagaaattta tgaaaaatat ggattagctg
    16861 aaatggaagt tacagaccaa atctttagaa gtgaacattc aaaagtgttt gatcaagctg
    16921 aaaatagaat gcatacaatt aaggcagtaa tggcagcaac attggggagt taatcactaa
    16981 atggaacgat atgaatatga tgtgtctgat gatataagtg tcatgtacag acacctcata
    17041 ttggtattaa aggagaaatg aatatgaacg aatcaggaga taacaaactc agtaaatctt
    17101 ctttaattgg actagttata ggatccatga ttggtggcgg tgcgttcaat ataatgtctg
    17161 atatgggcgg taaagccggt ggattagcca ttattattgg ttggattatt acagctatag
    17221 gaatgatttc attagcgttc gtatttcaaa atttaaccaa tgaacggccg gagctagacg
    17281 gtggtattta tagttatgct caagcaggat ttggcgattt tgtaggattt atcagtgctt
    17341 ggggatattg gttctcagcg tttttaggca atgttgccta tgcaacacta ttgatgtcag
    17401 cagtaggtaa ctttttcccg atttttaaag gaggcaacac attaccaagt attattgtcg
    17461 cctcgttact actctggggt gtccatttct tgattttaaa aggcgttgaa acagcagcat
    17521 ttatcaatag tattgttact gttgcaaagt taatcccgat tttacttgta atcatatgca
    17581 tgataattgc attcaatttt gacactttta aaacaggctt tttcggtatg acgtcagagg
    17641 gtgtattgcc atttagttgg gcgagcacaa tgagccaagt taaaagtacg atgctagtga
    17701 cagtttgggt gtttatcggt atcgaaggtg cagtaatttt ttctagtaga gctaaaaata
    17761 aaaaagatgt aggtagtgcc acggttatag gacttatatc agttttaatt atctatttct
    17821 tattaactgt attagctcaa ggcgtgattt tgcaaaatca tatttctcaa ttagattcgc
    17881 caagtatggc acaggtgctt gcaactattg taggtggttg gggatctaca cttgtaaata
    17941 ttggtttaat tatttcggta ctaggtgcat ggttaggatg gacattgctt gctggtgaat
    18001 tacctttcat tgttgcaaaa gatggattat ttccaaagtg gtttgctaaa gaaaataaaa
    18061 atggagcacc tgtaaatgca ctgcttatta ccaatatatt agtacaatta tttttaataa
    18121 gtatgctatt tacacagagt gcgtatcaat ttgcattttc actagcatca agtgctattt
    18181 tataccctta catgttcagt gcattttacc aagttaaata cactttagag catcgacagc
    18241 aagcaactac taaacaatgg acgattggca tcatagcctc aatttatgct atatggctta
    18301 tatatgcagc aggtatcaat tacttattat tgacgatgtt actttatatt ccagctcttc
    18361 ttgtttattc aatcgttcaa aagaataatc agacacgttt gattaaatca gactatattc
    18421 tttttatgat tattatcgta cttgcagtta tcggattaat taagttattg atgggaacga
    18481 taaatgtttt ttaaaaggag cgacaaaaat atgaaagaga aaattgtcat tgcattaggc
    18541 ggtaatgcga tacagacaaa agaagcaaca gctgaagcac aacaaacagc tattagacgt
    18601 gcgatgcaaa accttaaacc tttatttgat tcaccagcgc gtattgtcat ttcacatggt
    18661 aatggcccac aaattggaag tttattaatc caacaagcta aatcgaacag tgacacaacg
    18721 ccggcaatgc cattggatac ttgtggtgca atgtcacagg gtatgatagg ctattggttg
    18781 gaaactgaaa tcaatcgcat tttaactgaa atgaatagtg atagaactgt aggcacaatc
    18841 gttacacgtg tggaagtaga taaagatgat ccacgattca ataacccaac caaaccaatt
    18901 ggtccttttt atacgaaaga agaagttgaa gaattacaaa aagaacagcc agactcagtc
    18961 tttaaagaag atgcaggacg tggttataga aaagtagttg cgtcaccact acctcaatct
    19021 atactagaac accagttaat tcgaacttta gcagacggta aaaatattgt cattgcatgc
    19081 ggtggtggcg gtattccagt tataaaaaaa gaaaatacct atgaaggtgt tgaagcggtt
    19141 atagataaag attttgctag tgagaaatta gcaacgctga ttgaagcaga taccttaatg
    19201 attcttacga atgtagaaaa tgtatttatt aactttaatg aacctaatca acaacaaatc
    19261 gatgatattg atgtagcaac actgaaaaaa tacgcggcac aaggtaagtt tgcggaagga
    19321 tcgatgttgc caaaaataga agctgcgata cgatttgttg aaagtgggga aaacaaaaaa
    19381 gttatcatta ccaatttaga gcaggcatac gaagctttga ttggtaataa aggtacacac
    19441 attcacatgt agcactttat cacgcgacaa aacattaaat atgtttctcc gttgattcaa
    19501 atgaaaaagt tgtctgctga cactttgcaa ggtttgaagg agtttaactt atgacagaaa
    19561 actttatttt gggtagaaat aataaattag aacatgaact aaaggcatta gcagattaca
    19621 ttaatatccc atatagtata ttacaaccat atcaaagtga atgttttgtc agacattata
    19681 cgaaaggcca agttatttat ttttcgccac aagaaagtag caatatttac tttttaattg
    19741 aaggtaatat tattagagaa cattacaatc aaaatggaga tgtatatcgt tattttaata
    19801 aagagcaagt attatttcca atcagtaact tatttcatcc gaaagaggtt aacgaattgt
    19861 gtacagcatt aaccgattgt acagttcttg gattgcctag agaattgatg gcttttttgt
    19921 gcaaagctaa tgatgatata tttttgacac tttttgcatt aataaatgat aatgagcagc
    19981 aacacatgaa ctataacatg gcattaacaa gtaaatttgc taaagatcga attattaaat
    20041 tgttatgtca tctatgtcag acagtagggt acgatcaaga tgaattttat gaaatcaaac
    20101 agtttttaac tattcaactc atgagtgata tggctgggat ttcccgggaa acagctggtc
    20161 atattattca tgaacttaaa gatgaaaaac ttgttgttaa agatcataaa aattggttag
    20221 taagcaaaca tttattcaat gatgtatgtg tttaatatac aatgtaaaat gaataagttg
    20281 aacatgaggt ctaacgtaca tttatacgtt aggccttttt tgctagcatg atgaataatt
    20341 taaaatgtta gttaaatttg attgttgaaa ttacagtaaa atttaaggtg atgaaaaatt
    20401 tagaacttct aagtttttga aaagtaaaaa atttgtaata gtgtaaaaat agtatattga
    20461 tttttgctag ttaacagaaa attttaagtt atataaatag gaagaaaaca aattttacgt
    20521 aatttttttc gaaaagcaat tgatataatt cttatttcat tatacaattt agactaatct
    20581 agaaattgaa atggagtaat atttttgaaa aaaagaattg attatttgtc gaataagcag
    20641 aataagtatt cgattagacg ttttacagta ggtaccacat cagtaatagt aggggcaact
    20701 atactatttg ggataggcaa tcatcaagca caagcttcag aacaatcgaa cgatacaacg
    20761 caatcttcga aaaataatgc aagtgcagat tccgaaaaaa acaatatgat agaaacacct
    20821 caattaaata caacggctaa tgatacatct gatattagtg caaacacaaa cagtgcgaat
    20881 gtagatagca caacaaaacc aatgtctaca caaacgagca ataccactac aacagagcca
    20941 gcttcaacaa atgaaacacc tcaaccgacg gcaattaaaa atcaagcaac tgctgcaaaa
    21001 atgcaagatc aaactgttcc tcaagaagca aattctcaag tagataataa aacaacgaat
    21061 gatgctaata gcatagcaac aaacagtgag cttaaaaatt ctcaaacatt agatttacca
    21121 caatcatcac cacaaacgat ttccaatgcg caaggaacta gtaaaccaag tgttagaacg
    21181 agagctgtac gtagtttagc tgttgctgaa ccggtagtaa atgctgctga tgctaaaggt
    21241 acaaatgtaa atgataaagt tacggcaagt aatttcaagt tagaaaagac tacatttgac
    21301 cctaatcaaa gtggtaacac atttatggcg gcaaatttta cagtgacaga taaagtgaaa
    21361 tcaggggatt attttacagc gaagttacca gatagtttaa ctggtaatgg agacgtggat
    21421 tattctaatt caaataatac gatgccaatt gcagacatta aaagtacgaa tggcgatgtt
    21481 gtagctaaag caacatatga tatcttgact aagacgtata catttgtctt tacagattat
    21541 gtaaataata aagaaaatat taacggacaa ttttcattac ctttatttac agaccgagca
    21601 aaggcaccta aatcaggaac atatgatgcg aatattaata ttgcggatga aatgtttaat
    21661 aataaaatta cttataacta tagttcgcca attgcaggaa ttgataaacc aaatggcgcg
    21721 aacatttctt ctcaaattat tggtgtagat acagcttcag gtcaaaacac atacaagcaa
    21781 acagtatttg ttaaccctaa gcaacgagtt ttaggtaata cgtgggtgta tattaaaggc
    21841 taccaagata aaatcgaaga aagtagcggt aaagtaagtg ctacagatac aaaactgaga
    21901 atttttgaag tgaatgatac atctaaatta tcagatagct actatgcaga tccaaatgac
    21961 tctaacctta aagaagtaac agaccaattt aaaaatagaa tctattatga gcatccaaat
    22021 gtagctagta ttaaatttgg tgatattact aaaacatatg tagtattagt agaagggcat
    22081 tacgacaata caggtaagaa cttaaaaact caggttattc aagaaaatgt tgatcctgta
    22141 acaaatagag actacagtat tttcggttgg aataatgaga atgttgtacg ttatggtggt
    22201 ggaagtgctg atggtgattc agcagtaaat ccgaaagacc caactccagg gccgccggtt
    22261 gacccagaac caagtccaga cccagaacca gaaccaacgc cagatccaga accaagtcca
    22321 gacccagaac cggaaccaag cccagacccg gatccggatt cggattcaga cagtgactca
    22381 ggctcagaca gcgactcagg ttcagatagc gactcagaat cagatagcga ttcggattca
    22441 gacagtgatt cagattcaga cagcgactcg gaatcagata gcgattcaga atcagatagc
    22501 gactcagatt cagatagcga ttcagattca gatagcgatt cagaatcaga tagcgattcg
    22561 gattcagaca gtgattcaga ttcagacagc gactcagaat cagatagcga ctcagaatca
    22621 gatagtgagt cagattcaga cagtgactcg gactcagaca gtgattcaga ctcagatagc
    22681 gattcagact cagatagcga ttcagactca gacagcgatt cagattcaga cagcgactca
    22741 gaatcagaca gcgactcaga ctcagatagc gactcagact cagacagcga ctcagattca
    22801 gatagcgatt cggactcaga cagcgattca gattcagaca gcgactcaga ctcggatagc
    22861 gattcagatt cagacagcga ctcagactcg gatagcgact cggattcaga tagtgactcc
    22921 gattcaagag ttacaccacc aaataatgaa cagaaagcac catcaaatcc taaaggtgaa
    22981 gtaaaccatt ctaataaggt atcaaaacaa cacaaaactg atgctttacc agaaacagga
    23041 gataagagcg aaaacacaaa tgcaacttta tttggtgcaa tgatggcatt attaggatca
    23101 ttactattgt ttagaaaacg caagcaagat cataaagaaa aagcgtaaat acttttttag
    23161 gccgaataca tttgtattcg gtttttttgt tgaaaatgat tttaaagtga attgattaag
    23221 cgtaaaatgt tgataaagta gaattagaaa ggggtcatga cgtatggctt atatttcatt
    23281 aaactatcat tcaccaacaa ttggtatgca tcaaaatttg acagtcattt taccggaaga
    23341 tcaaagcttc tttaataacg atataactgc taaaccatta aaaactttaa tgttgttaca
    23401 tggattatca agtgatgaaa cgacatatat gagatataca agcatagaaa ggtatgcgaa
    23461 tgaacacaaa ttagctgtga ttatgcccaa tgtggatcat agcgcatatg ctaacatggc
    23521 atatggtcat agctattatg attatatttt ggaagtatat gattatgttc atcaaatatt
    23581 tccactttcc aaaaagcgtg atgacaattt tatagcaggt cactctatgg gaggatatgg
    23641 cacaattaaa tttgcattaa cacaagggga taaatttgcc aaagctgtac cattatctgc
    23701 tgtgtttgaa gcgcaaaatt taatggatct agagtggaat gatttttcaa aagaggccat
    23761 aattggcaat ctttcaagtg ttaaaggaac tgaacatgat ccgtattatt tgctagacaa
    23821 agctgtagct gaagataaac aaattccaaa attgctcatt atgtgtggta aacaagactt
    23881 tttatatcaa gacaacttag attttatcga ttatttatca cgtaaaaatg ttccttatca
    23941 atttgaagat ggaccaggag atcatgatta tgcatattgg gaccaagcga ttaagcgtgc
    24001 tataacatgg atggtgaatg attaattatt tcctggaaaa tatgtggttg cattaaatac
    24061 acagagtgag agatacaaac tatttacgca cgactaacat ttctgagtgt ttaaattaaa
    24121 tttgtattaa tatgattggc gcaatttgct gatacgcaaa aatgtttctc gtgaaactta
    24181 gatttagctt atagttttat catcatttgt atgacttaca ttataaattt tattataatg
    24241 aggttaacgc tttgaaagga gtcatcatca tgtcgaccaa taaaaacgat tatgagcata
    24301 tgttgtttta ttttgcatat aaaacgttta ttactaccgc tgatgaaatt atagagaagt
    24361 atggtatgag ccgtcagcat catcgttttt tgttttttat caataaatta cctggtatta
    24421 ctattaaatc attactagaa atattagaaa tttctaaaca aggatcacat gcaacacttc
    24481 aaaaattaaa agagcaaggt ctcattattg aaaaagtttt agagactgat cgacgtgtca
    24541 aaaaattata ttcgacggat aaaggcgatc aactcattgc tgaattgaac aaggcgcaag
    24601 atgaattatt gcaaaatata tatcaacaag tcggttcgga ttggtatgat gtgatggaag
    24661 cattggctaa agggcgacct ggctttgatt ttattaagca tttgaaagat gaaaaagaaa
    24721 gctagcatca gaaatgttaa aaatcttcgc attcttaaat ttaaaaaata tgtcaaaaag
    24781 tgtataataa aaacatataa tttaattgaa ctcagtttca acacatctta gaaaggagtt
    24841 tgaatgatga aaaaattagc agttatttta acattagttg gcggtttata ctacgcattt
    24901 aaaaaatacc aagaacgtgt taaccaagca cctaacattg agtactaaat taaacca
//
LOCUS       DAOBBH010000021        24037 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.021, whole genome shotgun sequence.
ACCESSION   DAOBBH010000021 DAOBBH010000000
VERSION     DAOBBH010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 24037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24037
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.021"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..217)
                     /locus_tag="P6L01_000581"
     CDS             complement(<1..217)
                     /locus_tag="P6L01_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDP3029670.1"
                     /translation="MAINIIEYNRSYKEELIEFILSIQKNEFNIKIDRDDQPDLENIE
                     HNYLNSGGQFWLAINNHQNIVGTIGLIR"
     gene            complement(493..1194)
                     /gene="isaA"
                     /locus_tag="P6L01_000582"
     CDS             complement(493..1194)
                     /gene="isaA"
                     /locus_tag="P6L01_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase IsaA"
                     /protein_id="HDP3029671.1"
                     /translation="MKKTIMASSLAVALGVTGYAAGTGHQAHAAEVNVDQAHLVDLAH
                     NHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADY
                     TTSYNQGSDVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSS
                     AAQIMAQRTGVSASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINA
                     AVKAYKAQGLGAWGF"
     gene            complement(1802..2857)
                     /locus_tag="P6L01_000583"
     CDS             complement(1802..2857)
                     /locus_tag="P6L01_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDP3029672.1"
                     /translation="MTSIKTITPKDFIFRVLSGVAIGIVAGLVPNAILGEIFKYFMDY
                     HPIFKTLLGVVVAIQFTVPALIGALVAMKFDLSPLAIAVVASAAYVGSGAAQFKNGAW
                     MITGIGDLINTMITAAIAVLFILLIQHRVGSMALIVFPTVVGDISGAIGVLILPYTKM
                     ITTAIGNMVNGFTELQPIVMSILISMVFSLIIISPLSTVAIAFAIGITGLAAGSASIG
                     ISATEAVLIIGTSKVNRLGVPLSVFFGGVKMMIPNMVKYPILMLPILTTAIVSGLVSA
                     LVGIHGTKESAGFGFIGMVGPINAFKFMEVDSAWLSVLLIVVAFFVVPFVTAWLADII
                     YRKVFRLYTNDIFKFMG"
     gene            complement(2984..3112)
                     /locus_tag="P6L01_000584"
     CDS             complement(2984..3112)
                     /locus_tag="P6L01_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029673.1"
                     /translation="MDFKNEDKIKVYCNNMFEKLPISLSKNSEFCDILFFIVLSYR"
     gene            3267..3836
                     /locus_tag="P6L01_000585"
     CDS             3267..3836
                     /locus_tag="P6L01_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDP3029674.1"
                     /translation="MMESKSIDPRIVRTKQLLVDAFLKISREKKLSQITVKDITDIAT
                     LNRATFYAHFTDKEDLLDYTLSVTILKDLNDNLSISNVINEKVLRNIFISIASYIKDA
                     AKSCELNSEAFCNKAHQRINNELEDIFAIMLENSYPEHQRDIIVNSASFLAAGISGLA
                     LHWFNTSQETADVFIDRNLPFLIHHIAHF"
     gene            4034..4534
                     /locus_tag="P6L01_000586"
     CDS             4034..4534
                     /locus_tag="P6L01_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029675.1"
                     /translation="MTSIFVFAYLNNTSGNMLFRQQMICACLLANVRHSSIVCLNNIC
                     CIVDMRCLNTKIGGIDVSETDEPQGFERTHNILNINQSSLGVVTYITNKLKSTLKQHI
                     IIARGKKRIDYRLSYNFYTTYYDNVEIKKIDCEYTYAMPIAILIRHTMSFDNAHFFAQ
                     LRHPVL"
     gene            4754..4984
                     /locus_tag="P6L01_000587"
     CDS             4754..4984
                     /locus_tag="P6L01_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDP3029676.1"
                     /translation="MKILDRINELANKEKVQPLTVAEKQEQHALRQDYLSMIRGQVLT
                     TFSTIKVVDPIGQDVTPDKVYDLRQQYGYIQN"
     gene            complement(5286..5663)
                     /locus_tag="P6L01_000588"
     CDS             complement(5286..5663)
                     /locus_tag="P6L01_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDP3029677.1"
                     /translation="MIQSMWFNLHVQDLEKSAQFYKALGFKINRNPQMLDKMVGIQIG
                     QTTVILIENKHFQNVSQQSLNTEPNEVMISLGVNTNEEVDQLVNKVKEAGGAVVQEPT
                     VSQGFYGAMFKDLDGHHFNFLVC"
     gene            complement(5680..6501)
                     /locus_tag="P6L01_000589"
     CDS             complement(5680..6501)
                     /locus_tag="P6L01_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HDP3029678.1"
                     /translation="MKDILVIGATGKQGNAVVKQLLEDGWYVSALTRNKNNRKLSDIG
                     HPHLSIVEGDLSDNVSLQSAMKGKYGLYSIQPIVKDDVSEELRQGMKIIEIAEQENIQ
                     HIVYSTAGGVNRNRTGPHFEVLAKIENRLMESNINATVIKPSFFMDNFLRIAKVEDER
                     ITLPEFINPNIKFTMISSIDIAKIASYIFAHPQSFTHQSIEIGSDEVTLSEAATIFSE
                     VTGKSTVIEGEFVSGVAEKQWLEEKGYEVDFELMAEINPTRLSLSDWLKVQNYNK"
     gene            complement(6521..6820)
                     /locus_tag="P6L01_000590"
     CDS             complement(6521..6820)
                     /locus_tag="P6L01_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HDP3029679.1"
                     /translation="MSLNKEQRRITAEELQAHFEESTLSVQMIAEKLNVTTEDVEKVL
                     AMTAPLGIFSHQLQRFIHLVWDVRDVINDNIKGNGQTPEPYTYLKGEKEDYWFLR"
     gene            6951..7508
                     /locus_tag="P6L01_000591"
     CDS             6951..7508
                     /locus_tag="P6L01_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDP3029680.1"
                     /translation="MRKDAKENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTL
                     YRHFKDKSDLCYYVIQRDLDIFITHFKQIKDDYHSNYEVMQVSLDYLLQFKIDNKALL
                     QCIEAGNNKLRFYQSAFYQELFDFYYDLFKSDDDTYTKFKTDMLLQSLSTSVFAFQIE
                     HRHISIEAYRNYLLNIYLDEVERND"
     gene            7501..8205
                     /locus_tag="P6L01_000592"
     CDS             7501..8205
                     /locus_tag="P6L01_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDP3029681.1"
                     /translation="MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQ
                     KLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSAPSKLTPRDVEEV
                     YQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSS
                     KSAVTMLTLQYAKGLPNMQINAADPGATNTDLVGDFSNNSKHVSEGIKPIIQLATIGA
                     DGPTGTFINGDGEMPW"
     gene            8303..9313
                     /locus_tag="P6L01_000593"
     CDS             8303..9313
                     /locus_tag="P6L01_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDP3029682.1"
                     /translation="MKSITFEEHYVIEDIQKETMNAISADPKGVPMKVMLEGLEKKTG
                     FTNADELSHHDERIQFMNNQDVQIQVLSYGNGSPSNLVGQKAIELCQKANDQLANYIA
                     QYPNRFVGFATLPINEPEAAAREFERCINDLGFKGALIMGRAQDGFLDQDKYDIIFKT
                     AENLDVPIYLHPAPVNSDIYQSYYKGNYPEVTAATFACFGYGWHIDVGIHAIHLVLSG
                     IFDRYPKLNMIIGHWGEFIPFFLERMDEALFAEHLNHSVSYYFKNSFYITPSGMLTKP
                     QFDLVKKEVGIDRILYAADYPYIEPEKLGVFLDELGLTDEEKEKISYTNGAKLLGLSS
                     NN"
     gene            9333..10163
                     /locus_tag="P6L01_000594"
     CDS             9333..10163
                     /locus_tag="P6L01_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDP3029683.1"
                     /translation="METLELQGAKLRYHQVGQGPVLIFIPGANGTGNIFLPLAEQLKD
                     HFTVVAVDRRNYGESELTEPLPDSASNPDSDYRVKRDAQDIAELAKSLSDEPVYILGS
                     SSGSIVAMHVLKDYPEVVKKIAFHEPPINTFLPDSTYWKDKNDDIVHQILTEGLEKGM
                     KTFGETLNIAPIDAKMMSQPADTEEGRIEQYKRTMFWSEFEIRQYTHSDITLDDFTKY
                     SDKITLLNGTDSRGSFPQDVNFYINKETGIPIVDIPGGHLGYIQKPEGFADVLLNMWG
                     "
     gene            complement(10310..11200)
                     /locus_tag="P6L01_000595"
     CDS             complement(10310..11200)
                     /locus_tag="P6L01_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDP3029684.1"
                     /translation="MKIVIIGGFLGGGKTTVLNHLLAESLKESLKPAVIMNEFGKMSV
                     DGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLD
                     ACLTPILAPFTTITHMIGVIDASMYKHIKSFPKDIQGLFYEQLAYCSVLFVNKIDSAD
                     VETTSKLLKDLEVINPEADIQVGMHGSVTLPISVRQMTATSDNKHESLHKTINHQFVQ
                     SPVTCTKAEFIKRLACLPSHIYRLKGFMTFEDTTHTYLIQFTQGQYELTPVAFSKKVP
                     EYLVLIGKGISKEDYQCLEQ"
     gene            complement(11297..12637)
                     /locus_tag="P6L01_000596"
     CDS             complement(11297..12637)
                     /locus_tag="P6L01_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HDP3029685.1"
                     /translation="MLEVKYDMRTGHYTPIPHEPHYLVISHADKLTATEKAKLRLLII
                     KQKLDISLAESVVSSPIASEHVIEQLTLFQHERRHLRPKISATFLAWLLIFLMFALPI
                     GIAYQFSDWFQNQYVSAWIEYLTQTTLLNHDILQHILFGDYGVLSLGTYSLVWALPVV
                     ILISLSTAIIDQTGLKSWMIWAIEPSMLWIGLQGNDIVPLLEGFGCNAAAISQAAHQC
                     HTCTKTQCMSLISFGSSCSYQIGATLSIFSVAGKSWLFMPYLILVLLGGILHNRIWLK
                     KNDQQLSVPLPYDRQLHMPNIRQMLLQMWQNIQMFIVQALPIFITICLIVSILSLTPI
                     LNVLSQIFTPILSLLGISSELSPGILFSMIRKDGMLLFNLHQGALLQGMTATQLLLLV
                     FFSSTFTACSVTMTMLLKHLGGQSALKLIGKQMVTSLSLVIGVGIIVKIVMLII"
     gene            complement(12631..13737)
                     /locus_tag="P6L01_000597"
     CDS             complement(12631..13737)
                     /locus_tag="P6L01_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HDP3029686.1"
                     /translation="MQHHKVAIIGAGAAGIGMAITLKDFGITDVIILEKGTVGHSFKH
                     WPKSTRTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYEL
                     NIFENTVVTNISADDAYYTIATTTETYHADYIFVATGDYNFPKKPFKYGIHYSEIEDF
                     DNFNKGQYVVIGGNESGFDAAYQLAKNGSDIALYTSTTGLNDPDADPSVRLSPYTRQR
                     LGNVIKQGARIEMNVHYTVKDIDFNNGQYHISFDSGQSVHTPHEPILATGFDATKNPI
                     VQQLFVTTNQDIKLTTHDESTRYPNIFMIGATVENDNAKLCYIYKFRARFAVLAHLLT
                     QREGLPAKQEVIENYQKNQMYLDDYSCCEVSCTC"
     gene            complement(14070..14366)
                     /locus_tag="P6L01_000598"
     CDS             complement(14070..14366)
                     /locus_tag="P6L01_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HDP3029687.1"
                     /translation="METIGSIIYLKEGSQKLMIINRGPIVEIENQKYMFDYSACKYPI
                     GVVEDEIYYFNEENIDSVIFKGYSDQDEVRFQELFENMKQNLDSEIQRGEVTPQ"
     gene            complement(14354..14722)
                     /locus_tag="P6L01_000599"
     CDS             complement(14354..14722)
                     /locus_tag="P6L01_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029688.1"
                     /translation="MSNKLDGINKMITAKHKQMDDLYDEKREVKALIDESDELNHSIE
                     QLYQHLGDRYHSSNMASRMEQFRDEFHFAKRRSTEALYEQQQQIQHGIRKAEEEMIDL
                     EMRRNVEIETVTKEENKWKQ"
     gene            complement(14737..15015)
                     /locus_tag="P6L01_000600"
     CDS             complement(14737..15015)
                     /locus_tag="P6L01_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HDP3029689.1"
                     /translation="MMSTVQSDIFKTNSASSSIKSAVETCNNVSKPDKDESTTVSGNN
                     NAHSVIDDLISKNQSVAEAIRNASDSIQKVGEEFNQTDVMIGNEIGKN"
     gene            complement(15356..16222)
                     /locus_tag="P6L01_000601"
     CDS             complement(15356..16222)
                     /locus_tag="P6L01_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDP3029690.1"
                     /translation="MNEQWLEHLPLKDIKEISPVSGGDVNEAYRVETDTDTFFLLVQR
                     GRKESFYAAEIAGLNEFERAGITAPRVIASGEVNGDAYLVMTYLEEGASGSQRQLGQL
                     VAQLHSQQQEEGKFGFSLPYEGGDISFDNHWQDDWRTIFVDKRLDHLKEELLNRGLWD
                     ANDIKVYDKVRSQIVEELEKHQSKPSLLHGDLWGGNYMFLKDGRPALFDPAPLYGDRE
                     FDIGITTVFGGFTSEFYDAYNKHYPLAKGASYRLEFYRLYLLMVHLLKFGEMYRDSVA
                     HSMDRILQGTTS"
     gene            16440..17504
                     /locus_tag="P6L01_000602"
     CDS             16440..17504
                     /locus_tag="P6L01_000602"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDP3029691.1"
                     /translation="MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTY
                     KNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGALELGGITPKPQPGNPQP
                     RMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYI
                     KVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTTFKKQHDVTVPIYLKL
                     TSDMDFDGLKALLPAITETFDGIILANTTRQRDGLTSANKVEEGGLSGRPLFERNLKL
                     IKYAYQQTNGEFLIIGTGGVFSTEDAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGI
                     ARYLKDHHFDNVSDIIGLDA"
     gene            17659..17934
                     /locus_tag="P6L01_000603"
     CDS             17659..17934
                     /locus_tag="P6L01_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029692.1"
                     /translation="MTKETDNKKTVATSDNKDTQQQNQTEEDERQGLNGYRKTDLDLE
                     IEQELREMMKTGENETKNDYKKFKVFSLISTLVIVILAIIRFVHKMI"
     gene            complement(18120..18842)
                     /locus_tag="P6L01_000604"
     CDS             complement(18120..18842)
                     /locus_tag="P6L01_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDP3029693.1"
                     /translation="MINAEPIISKMKNQKINYDKVLKKLIGQWEREAIRPKILLHSCC
                     APCSTYTLEFLTQYADIAIYFANSNIHPKNEYLRRAKVQEQFVEDFNRKTGANVKYIE
                     APYEPHKFVKMVKDKELADEKEGGLRCTACFEMRLDIVAKAAVEHGYDYFGSAITLSP
                     KKNAQLINELGMDVQKIYDVNYLPSDFKKSKGYERSIEMCNDYNIFRQCYCGCVFAAM
                     QQGIDFKTVNKEAKAFLEQYPD"
     gene            19196..19645
                     /locus_tag="P6L01_000605"
     CDS             19196..19645
                     /locus_tag="P6L01_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HDP3029694.1"
                     /translation="MTALFPYIAFENSKEALAYYEEVFGATDVKRLEVGEEQASHFGM
                     TKEEAQEATMHAEFEVLGVKVLCSDSFGRADKINNGISLLIDYDVNNKEDADKVEAFY
                     EKIKDHSSIEIELPFADQFWGGKMGVFTDKYGVRWMLHGQDYTAIQQ"
     gene            complement(19726..20313)
                     /locus_tag="P6L01_000606"
     CDS             complement(19726..20313)
                     /locus_tag="P6L01_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDP3029695.1"
                     /translation="MTIDRRIRKSQVAMRNAFIELLHQHQLEEITVQQIADLADVNRS
                     TFYTHYYDKYDLLEKLEDQQLEEIRDFIHDEKLNGAVKLSTDNIHQIMTVLIDMIEKD
                     IAFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSLI
                     RHWVEDPNRIDKVDLIMHFNTIVNQGPATIIKNIQ"
     gene            complement(20291..20399)
                     /locus_tag="P6L01_000607"
                     /pseudo
     CDS             complement(20291..20399)
                     /locus_tag="P6L01_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501367.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20456..22138
                     /locus_tag="P6L01_000608"
     CDS             20456..22138
                     /locus_tag="P6L01_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HDP3029696.1"
                     /translation="MNQHLLGNPKLTVTHVNEVKTGINHIVVDSVQYGNQEMIMEKDV
                     TVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGAIPTSSF
                     TPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAAKQPWSN
                     GNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWI
                     QGIFARWTDNPNIEDMIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNR
                     GSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVI
                     YEVRDQFYKGEFKSASAFPLPNTEYTPLYLNAENHTLNHAKISSAYVAQYDSEDKRQD
                     VSFKYTFDKDTELVGNMNLKLWVSTTDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENG
                     QVATGWLRVSHRELDQEKSTIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGET
                     LEVVVKGSEIVIGNSTPGMKTRYEHEETINNGMHMIYTGGKYDSQLIIPIVN"
     gene            complement(22236..22871)
                     /locus_tag="P6L01_000609"
     CDS             complement(22236..22871)
                     /locus_tag="P6L01_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDP3029697.1"
                     /translation="MKKVISLFVAILSVLIFTDNANAENNKPEGVNENSNLIPVRQPD
                     ANYPGPVSDIARVKKLDNLIDGTKDPDVIKQLKDDNEVPNNAVKAKDSKIVSDCRHLL
                     KHPDNLIDGTKDPNVINQLKNANGTSNNSAVNPSIKANDIHTKSNKQMAKQQSIHLDS
                     KSKSVHKQQLKELPKTGVNNESNTAIMALFIGLILLVVSLVTRFIKLNSHQ"
     gene            complement(22887..23963)
                     /locus_tag="P6L01_000610"
     CDS             complement(22887..23963)
                     /locus_tag="P6L01_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HDP3029698.1"
                     /translation="MTKIFKISFLILLVVIVSSYYGESFVLADNNPIVNSENGSDNNF
                     ESGTIERNQNELYEVGDYSTPLTERVFGKDQRTVVNNILQRPYKQTVLLNMTFSNNRV
                     YKGTGTMIGKDIVLTAAHNVYSKDDKGWAKKIDVYAGVNGQTYTIGKAFSHKFFVSKT
                     WINNAPTKEDIAIIKLNSNLGNKTGYLTLNTHISKGENIEISGFPGDKSDNRQYKGKG
                     KLESFDENEMYYTVDTFSGQSGSAIRDSKNNIIGVHAYGRYNHNSGVRINDLKLDYIN
                     YWIGKYRSHPYNKKVKVIKSKYIYWKNIEITKKGDNKLIKKDKAYTAKLYYNIPNGYK
                     YYSLYDEKNRWMGYFNSNDIKVVK"
BASE COUNT         7874 a         4343 c         3708 g         8112 t
ORIGIN      
        1 gtctaatcaa acctatagta ccaacaatat tttgatgatt gtttattgca agccaaaatt
       61 gccctccact attcaaatag ttatgttcga tgttctccaa atcaggttga tcatctctat
      121 caatttttat attaaattca tttttttgaa tcgataaaat aaactcgatt agctcttcct
      181 tataagacct attatattca attatgttta tagccatttt tatctccttt ttcatttaat
      241 ttaatcataa aatgcgcgtt tagtttgtat ctagtgtact cagtacagcc tcaaatgaag
      301 ttttattcca cttggcactt aataaagaca agtattttag cagtaataca ataaagtcca
      361 ataaatttcc ctaacttcaa tatccacttt ttaagaaatg tatttttaat taataaaaaa
      421 actctcccca atttctatgg gaagagctat atatttaatg tctaaacatt acttttattt
      481 attatgaagg aattagaatc cccaagcacc taaaccttgt gctttgtatg ctttaacagc
      541 tgcgttgatt tgttggtcaa cagtgtttgt cggaccccaa cctggcatag tttggaataa
      601 acctgaagca cctgatgggt tgtaagcatt tacttgacca tttgattcac gagcgatgat
      661 tgcagcccat gtagaagctg aaacaccagt acgttgagcc atgatttgag ctgctgatga
      721 accagtagca cctgcagtat taccattgct taatctcact gaacttgaag tagttgaagt
      781 gctgtagtta tggtaagttg gagctgaaac agcttcaacg tttgagttac ttgattgtgc
      841 attgtagctt actgattgta catctgaacc ttggttgtat gaagtagtgt agtctgcacc
      901 tgcaacgttt gagaaaccag cagtttgacc attagctgct tcatagctcc atgaccatgt
      961 agtaccattt gaagtaaagt tatattggaa accatctttt acaaagtgga tgtcatatgc
     1021 accatctttg attggagctg catttaattg atcttggtga ttatgcgcta agtcaactaa
     1081 gtgtgcttga tcaacgttta cttcagcagc gtgtgcttga tgtcctgtac ctgctgcgta
     1141 acctgttaca cctaatgcca ctgctaatga tgatgccata attgtctttt tcatagtaaa
     1201 aaatcctcca gtaataattg taagtttatg tttttagtaa ttatattttg aatttgaatg
     1261 tcgtagtgca agtttaaatt gtcttttatt tctttcaacg gtactcacta tatcacaaaa
     1321 aaccagccag taaattacac tttctttaca aaacattaca atatcaagtg ttatttgtaa
     1381 tgttgaaata tggctgtttt atactgtaat gtgaaatatg tgccctttag aatccaatca
     1441 acccttgaaa tagtctttaa cacataagat ttttactata tttagctcaa ctattacagc
     1501 tttcgtaata ttacagattg tatttttgtt acatagctgt aatatatctg acatgtaact
     1561 tctcttattt tcgtacatta aacgcaatta aaaagcaatc aacacatatg tttctgcaca
     1621 tgtattgatt gctattattg ttgtatattc aaagttttaa aacacacatc ttttgtgaat
     1681 tatcttatct tttataagca caaataaact gcaactcaat tatattgcta aactttattc
     1741 tcgcaattca caataacatt aaataatttt tggtctaata ttttcaaaaa acatactgtc
     1801 cttatcccat gaatttaaaa atatcattag tatataaacg aaacacttta cgataaatga
     1861 tatctgcaag ccaagctgtt acaaatggca caacaaagaa cgctactaca attagtaaga
     1921 cactcaacca agcagaatca acctccataa atttaaatgc attaatcggt cctaccattc
     1981 ctataaaacc aaatccagct gattctttcg ttccatgaat acctactaat gctgatacca
     2041 aacctgatac aatggctgtt gtcaatattg gtaacataag aattggatat ttcaccatat
     2101 taggtatcat cattttaacg cctccaaaga agacggataa cggcacccct aaacgattca
     2161 ctttacttgt accaattatc aatactgctt cagtcgcgga gataccaatt gacgctgatc
     2221 cagctgctaa acctgtaata cctatcgcaa aggcaatggc cacagttgat agtggcgaaa
     2281 taataataag actaaatacc attgaaatca aaatactcat gacaatcggt tgtaattctg
     2341 taaaaccatt aaccatatta ccgatggctg ttgtaatcat tttcgtatac ggcaatatta
     2401 aaacaccaat tgcacctgaa atatcgccaa caactgttgg gaatacaatc aatgccatac
     2461 tacctacacg atgttgaata agtaaaatga acaacactgc aatcgctgct gtaatcattg
     2521 tattaattaa atcaccaata cccgtaatca tccaagcacc atttttaaac tgcgctgcac
     2581 cgcttcctac atatgctgca cttgccacaa cagcaattgc taatggcgat aggtcaaatt
     2641 tcatggcaac caatgcacca atcaaagcag gtactgtaaa ttgaattgca acgacaacgc
     2701 ctaataacgt tttaaaaatc ggatgataat ccataaagta tttaaaaatt tctccaagta
     2761 tcgcattagg aactaaaccc gcaacaatac ctatggcgac acctgataaa actctaaata
     2821 taaaatcttt gggtgtaatt gttttaattg atgtcataat atcatccttc catttatgta
     2881 tatacatctg tatgcaaata ataaagagcc ttaagttata agctgccact agcttaaatt
     2941 ctaagatgtg catgccgatg ttgttatatt taggctagca gtatcatcta taactcaaga
     3001 ctatgaaaaa tagtatatca caaaattctg aatttttaga taaagaaatt ggcaattttt
     3061 caaacatatt gttacaatac acttttattt tatcttcatt tttaaaatcc attaatacaa
     3121 tagaagaaag acattcaaat gcttaccaaa aaggtacatt atttgttagg agcgtatcaa
     3181 cacttacata tcatcaacac aattgacaat ataatagaag atactgataa taagtgttaa
     3241 aacaacagat gttaggtagt gaacaaatga tggaaagtaa atccatagat ccaagaatcg
     3301 ttagaaccaa acaattgctt gtcgatgctt ttcttaaaat ttctagagaa aagaaattat
     3361 ctcaaattac agttaaagat atcactgata ttgctacgct aaatcgtgca acattttacg
     3421 ctcatttcac tgataaagaa gacctcctag actacacatt atctgtaacc attttaaaag
     3481 acttgaatga taacttgagc atttctaatg tcattaatga aaaggtgctg cgtaatattt
     3541 tcatttcaat tgcgagttat atcaaagatg ctgcaaagtc ttgcgaatta aatagtgaag
     3601 cattttgcaa caaagcacat caacgtatta ataatgaatt agaagatatt tttgcgatta
     3661 tgttagaaaa cagctatccg gagcatcaac gagatatcat tgtaaatagt gcgagttttt
     3721 tagcagctgg tatctcaggc ttagcattac attggtttaa cacgagtcaa gagacagccg
     3781 atgtgtttat cgatcgcaac cttccatttt taattcatca tatagcacat ttttaataaa
     3841 acttggtatt tagtcatgca tcttgaaatc actatgtgac ttaggttcat acttgtacac
     3901 acaataaaat ttaacatatt acgattgatt agccgtgtct aggacataaa tcaacgtcct
     3961 atactctaca atgtcatatt agcagtcgtt aactgaatga aaataagctt gtcattaaaa
     4021 catatagatt ttagtgacaa gcatttttgt ttttgcgtac ttaaacaaca cttcaggcaa
     4081 tatgttgttt aggcaacaaa tgatatgtgc gtgtttattg gcaaacgtac gacatagtag
     4141 tatagtatgt ctaaacaaca tatgttgcat agttgatatg cgttgtttaa atactaagat
     4201 aggagggatt gacgtgagcg agacagatga acctcagggg tttgaacgca cgcataatat
     4261 attaaatatt aatcagagta gtctgggtgt agtgacatac attacaaata aattaaagtc
     4321 gacgttgaag caacacataa taattgctcg tggtaaaaag cgaatcgact atcgactgtc
     4381 gtataacttt tacactacgt attatgataa tgtagaaatc aagaaaatcg actgtgaata
     4441 tacctatgct atgcccattg caattttaat aagacacacg atgtcattcg acaatgctca
     4501 tttctttgct cagttacgtc atcctgtctt ataaaacaac attgcagaca tgtatatcaa
     4561 acgacacttc aataacatca ctttgccatc gtactactat taaaatcgtg tctcaaaccc
     4621 cttattttaa ttccaaaaat ctgctggtca aaagaccgag aaactaaaaa cattactgga
     4681 tgtgttgata aattatctta tagaaataaa ctcaaaatat atcaacactt gattctaagg
     4741 aggatatgac aatatgaaaa ttttagatag aattaatgaa cttgcaaata aagaaaaagt
     4801 ccaaccactt actgtagctg aaaaacaaga acaacatgca ttgcgtcaag actacttaag
     4861 tatgatccga ggacaagtat taacaacatt ttccacaata aaagtggttg atccaatcgg
     4921 tcaggatgtc acaccagata aagtttatga tcttcgccaa caatacggtt atattcaaaa
     4981 ttaatatttg ctcacgaggt attgcactta aggtgccaac tgacctcata aacaaagccc
     5041 atactgattg aagacactaa tgtgtccagg ctgggacata aatcaatgtt ctatgctcta
     5101 cgaagttata ttggcagtag ttgactgaat gaaaatgcgc ttgtaacaag cttttttcaa
     5161 ttctagtcaa ccttgccggg gtgggacgac gaaataaatt ttgcgaaaat atcatttctg
     5221 tcccactccc acattaaact tcatctctgt atgggctttt tatttattct tttgagaatt
     5281 tcattttagc agactaaaaa attaaaatgg tgtccatcaa gatctttgaa cattgcacca
     5341 taaaaccctt ggcttactgt tggttcttga acgactgcgc cacccgcttc tttcacttta
     5401 ttcaccaact ggtcaacttc ttcatttgtg ttcacaccta gagaaatcat cacttcattt
     5461 ggttcagtat taaggctttg ctgacttaca ttttgaaaat gcttgttttc tattaaaatt
     5521 acggttgttt gacctatttg aataccgacc attttatcta acatttgtgg gtttctattt
     5581 attttaaatc ctaacgcttt ataaaactgt gcgctctttt ctaaatcttg cacatgcaaa
     5641 ttaaaccaca ttgattgaat catgattgca ccccattcat tacttattat agttttggac
     5701 tttaagccaa tcacttaatg ataatcttgt tggatttatt tcagccatta attcaaagtc
     5761 tacttcataa cctttttctt ccaaccattg cttttctgca acaccactaa caaattctcc
     5821 ttctataaca gtagatttac ctgtcacttc actaaaaatt gttgctgctt cacttaatgt
     5881 aacttcatcg gaaccaatct ctattgattg atgcgtaaag ctttgtggat gtgcaaaaat
     5941 atacgatgca attttagcta tatcaataga agaaatcatt gtgaatttta tattcggatt
     6001 aataaattct ggtaatgtaa tacgttcatc ttcgacttta gcaatgcgta aaaaattatc
     6061 cataaagaat gatggtttga taactgttgc atttatatta gattccatta atctattttc
     6121 tatttttgct agtacttcaa agtgtgggcc agttcgattt cgattaaccc ctcccgcagt
     6181 actatacaca atatgttgaa tattttcttg ctcagctatt tcaattatct tcataccttg
     6241 tcttaattct tcgctaacat catctttaac gattggctga atactgtata agccatactt
     6301 ccctttcatc gctgattgca aactaacatt atcactcaga tcaccttcaa cgattgataa
     6361 atgcggatgt cctatgtctg aaagtttacg attattctta tttctagtta atgcacttac
     6421 ataccatcca tcctctaaca actgttttac aactgcatta ccttgcttcc ctgttgcgcc
     6481 tattactaaa atatctttca ttttagatcc actcctcttt ttatcttaaa aaccaatagt
     6541 cctctttttc accttttaaa tacgtatatg gttctggtgt ttgtccattt cctttaatat
     6601 tgtcgtttat tacatctctg acatcccata ctaaatgaat aaatcgttgt aattgatgac
     6661 taaaaatgcc tagtggcgct gtcatagcta atactttttc cacatcttct gtagtgacat
     6721 tcagtttttc agcaatcatt tgaaccgata aggtagattc ttcaaaatgt gcttgcaact
     6781 cttcagctgt gatgcgtctt tgctctttat ttaaagacat tatcaacact cttttctttt
     6841 atgatattta atagttattg caaattcatt gctaactttc ttaacacgtt ttataataaa
     6901 cggatagtta tccgtttgtc aattttagag aattttaagt aggtgtaatc atgcgaaaag
     6961 atgcaaaaga gaatagacaa cgaattgaag aaatagcgca caaacttttt gatgaagaag
     7021 gcgttgaaaa cattagtatg aatcgtattg ctaaagaact aggtatcggt atggggacat
     7081 tgtatcgaca tttcaaagat aaaagcgatt tatgctacta cgtcatacaa agagaccttg
     7141 atatttttat cactcatttt aagcaaatta aagatgacta tcattctaat tatgaggtta
     7201 tgcaagtgtc gttagattat cttttacaat ttaaaatcga taataaggca ctactgcaat
     7261 gtattgaagc aggcaacaat aaattacgtt tttaccaaag cgcattttat caagaattat
     7321 ttgattttta ttatgactta tttaaaagcg atgacgacac ttacacgaag ttcaaaacag
     7381 atatgctgtt gcaatcatta tcaactagtg tatttgcctt ccaaatagag catcgccata
     7441 tttcaattga agcgtacaga aattatttat taaacattta tttagatgag gtggaacgta
     7501 atgactaaaa ttgtgttaat cacaggaggc aataaagggt taggctatgc aagtgcagag
     7561 gcacttaaag cattgggtta caaagtttat ataggttctc ggaatgatgt aagaggacaa
     7621 caagcatcac aaaaattagg tgttcattat gtacagttag atgttacaag tgactactca
     7681 gtcaaaaatg cttataacat gattgctgaa aaagaaggcc gtcttgatat cctcattaac
     7741 aatgcaggta tatctggtca attttcagca ccttccaaat tgacacctcg tgacgttgaa
     7801 gaagtatatc aaacgaatgt attcggtatc gtacgaatga tgaatacatt cgtccctctc
     7861 ttagaaaaat ctgaacaacc tgttgtcgtc aacgtatcaa gtggtttagg ttcatttgga
     7921 atggttacaa accccgaaac agctgaatct aaagtgaatt cattagctta ttgttcgtct
     7981 aaatcggcag taacaatgct aacgctacaa tacgctaaag gattacctaa catgcaaatc
     8041 aatgctgctg acccaggcgc tacaaatact gatttagttg gtgattttag taataattcc
     8101 aaacacgttt ctgaaggaat caagccaatc attcaattag caacgattgg tgcagatggc
     8161 ccaacaggta catttattaa tggcgatgga gaaatgcctt ggtagatcaa ttttatgtta
     8221 caaacattga aattagaggt atataaaaaa tgcttctgca atagatgcaa tatacatgca
     8281 aaacaatatg gaggcgaatt aaatgaaaag tattacgttt gaagaacatt atgtcattga
     8341 agatattcaa aaagaaacga tgaatgcgat atcagcagat cctaaaggtg taccgatgaa
     8401 agtaatgtta gaaggccttg aaaaaaagac aggttttaca aatgccgacg aattatcaca
     8461 tcatgatgaa cgtatccaat ttatgaataa tcaagacgtt caaattcaag tcttatctta
     8521 tggaaatgga tctccttcaa atttagttgg tcagaaagcc attgaattat gtcaaaaagc
     8581 aaatgatcaa ttggcaaact atattgccca atatcccaat cgctttgtag gctttgcaac
     8641 tttacctatc aatgagcctg aagccgcagc gcgagaattc gaacgttgca tcaatgattt
     8701 aggattcaaa ggtgcgctca ttatgggacg tgcacaagat ggttttcttg atcaagacaa
     8761 atatgacatt attttcaaaa cagctgaaaa tttagacgta ccgatttatc tacatcccgc
     8821 gccagttaac agtgacattt atcaatcata ctataaagga aattatcctg aagtaactgc
     8881 ggcaacattt gcttgttttg gttatggttg gcacattgat gtcggaattc atgcaataca
     8941 tctagtatta tctggcattt ttgatcgtta tccaaagtta aatatgatta ttggacattg
     9001 gggtgagttt atcccattct tcttagaacg tatggatgaa gctttattcg ctgaacattt
     9061 gaaccactct gtaagctatt actttaaaaa tagtttttat atcacaccaa gtggcatgtt
     9121 aacgaagcca cagtttgatt tagtcaagaa agaagtaggt attgatagaa ttctttatgc
     9181 tgctgattat ccatatattg agcctgaaaa attaggtgta tttttagatg aactgggttt
     9241 aacagatgaa gaaaaagaga aaataagtta tacaaatggt gctaaattat taggtttatc
     9301 atctaacaat taacaaatgg aggtcattga atatggaaac tttagaatta caaggcgcta
     9361 aattacgata ccaccaagtc ggacaaggac ccgtgctcat ctttattcct ggtgcaaacg
     9421 gtaccggaaa catttttctg cctcttgcag aacagttaaa agaccatttt actgttgtag
     9481 ccgttgatcg tcgtaattat ggagaaagcg agttaactga accactccct gattccgctt
     9541 caaaccctga cagtgattat cgtgtcaaac gcgacgcaca ggacattgcc gaacttgcaa
     9601 agtcattaag tgatgaacct gtctatatat taggttcaag ttcaggttca atcgttgcga
     9661 tgcatgtgtt aaaagattac cctgaagtcg ttaaaaagat tgcatttcac gaaccaccaa
     9721 ttaatacatt tttaccagac agtacttatt ggaaagataa aaatgatgac attgtgcatc
     9781 aaattttaac agaaggctta gaaaaaggta tgaaaacatt tggtgaaaca ttaaatatcg
     9841 caccaattga tgcaaaaatg atgtctcaac ctgctgatac tgaagaagga cgtatagaac
     9901 agtacaaacg tacgatgttc tggtcagaat ttgaaattag acaatatacg cattcagata
     9961 ttactttgga tgatttcaca aaatacagcg ataaaatcac cctactaaat gggacagatt
    10021 ctagaggctc cttcccacaa gatgttaact tttatataaa caaagaaact ggcataccta
    10081 ttgtagatat tccaggcggt cacttaggtt atattcaaaa acctgaaggc tttgctgatg
    10141 tattattaaa tatgtggggt taattgatcg ttctctaata aaacagcttg gacagttttc
    10201 aaattatatt tgatggctga ccaagcttat ttttgtttac gtttaaacgt tcaaatcaac
    10261 tcgttattat acatgaaggc atgttaacca ttctcttcca ctgaaaacac tactgttcca
    10321 aacattgata gtcttctttt gaaataccct ttccgataag caccaaatat tctggcactt
    10381 tttttgaaaa tgctacaggt gttaattcat attggccttg tgtaaattga atgagatacg
    10441 tatgtgtggt gtcttcaaat gtcataaacc ctttcaacct ataaatatga gacggaaggc
    10501 atgctaaacg ttttataaac tctgctttag tacatgtgac tggtgattgc acaaattgat
    10561 gatttattgt tttgtgtaat gattcatgtt tattgtcaga agttgctgtc atttgtctaa
    10621 ctgatattgg caaagtgacg ctgccatgca taccgacttg tatatcggcc tctgggttaa
    10681 taacttctaa atctttcaat agtttgctcg ttgtttcaac atctgctgaa tctattttat
    10741 taacaaataa gacagaacaa tatgctaatt gctcataaaa taagccttgg atgtctttag
    10801 ggaatgattt aatgtgttta tacatgcttg cgtctattac accaatcata tgtgtaattg
    10861 ttgtaaacgg agctaaaata ggcgttaaac aagcatctaa gacagagact ggttctgcaa
    10921 tcccactaca ttcaataaat acaatgtctg gttgctcttt taaatataat tgatgtaact
    10981 gttctgatac atctgctttc attgcgcaac agatacaccc ctctgtcagt tcacttaaag
    11041 gtatgtcttc agatactaag gcaccatcaa cactcatttt cccaaattca ttcatgatga
    11101 ctgctggttt cagcgattcc tttaatgatt cagcgagcaa atgatttaag acagtcgttt
    11161 taccgccacc taaaaaccca cctataataa ctattttcat ctgcgactct cctcttatac
    11221 ttcatatgaa tagcatattc caagatagat ctcctattga ctgacatgac tctatattca
    11281 gttatagttc atttttttaa ataatcagca ttactatttt aacaatgatg cctacaccaa
    11341 taactaaaga caatgatgtc accatttgct ttccaattaa ttttagtgct gactgaccac
    11401 ctaaatgttt caaaagcatc gtcattgtga ccgagcacgc tgtaaatgtt gaactaaaaa
    11461 acacaagtag tagtaactgt gttgctgtca ttccttgtaa taaggcgcct tgatgcaaat
    11521 taaacaagag catgccgtct tttcgaatca ttgaaaataa aatccctggt gacaattctg
    11581 aagagatgcc taataacgat aatataggtg taaatatttg tgataaaaca ttcaaaattg
    11641 gcgttagtga taaaatacta acaataagac agattgtgat aaaaataggt agcgcttgaa
    11701 cgataaacat ttgtatattt tgccacattt gtagcaacat ttgacgtata tttggcatat
    11761 gtaattgcct atcataaggt agcggaacac taagttgttg atcattcttt ttcaaccata
    11821 tcctattatg taagatgcca cctaaaagta ctaatattaa gtacggcata aatagccatg
    11881 actttccagc tacactaaaa atagataatg tcgcacctat ttgataacta caagaactac
    11941 caaagcttat taaactcata cactgtgtct tcgtgcaggt atggcattgg tgtgctgctt
    12001 gtgaaatagc tgctgcatta catccaaatc cttctaatag tggcacgata tcattacctt
    12061 gtaatccgat ccataacatt gacggttcaa ttgcccatat catccatgat ttgagtcctg
    12121 tttgatcaat tattgcagtt gataaactaa ttaatattac aaccggcaat gcccatacga
    12181 gcgaatatgt tccaagtgat agcacaccat aatcaccaaa taatatatgc tgtaatatat
    12241 cgtgattgag caatgttgtt tgagttaaat attctatcca tgctgataca tactgatttt
    12301 gaaaccaatc tgaaaattga taagcgatac caattggcaa tgcaaacatt aaaaatatca
    12361 acaaccaggc taaaaatgtc gcacttattt taggtcttaa atgtcgtcgc tcatgttgaa
    12421 atagtgtcaa ttgttctatc acatgttcac tcgctatagg cgaagaaact acactttctg
    12481 ccaatgaaat atctaatttc tgttttatga ttaataatct taatttcgct ttttctgttg
    12541 cggtaagttt atccgcatgg ctaataacca aataatgagg ttcatgaggt atgggcgtat
    12601 aatgcccagt tctcatatca tatttcactt ctaacatgtg catgacactt cacaacatga
    12661 ataatcatct aaatacattt gatttttttg gtaattttca atgacctctt gtttagctgg
    12721 taagccttcc cgctgtgtta aaagatgggc aagtactgca aatcgcgctc tgaatttata
    12781 gatatagcat aatttggcat tatcattttc aactgttgca ccaatcataa aaatattcgg
    12841 ataacgtgtc gattcatcat gtgttgttaa tttaatatct tgatttgttg tcacaaataa
    12901 ttgttgaacg attggatttt ttgttgcatc aaagccagtt gctagtattg gttcatgagg
    12961 cgtatgcaca ctttgtccgc tatcaaaact gatatgatac tgtccattgt taaaatcaat
    13021 atctttaact gtataatgta cattcatttc gatgcgagca ccttgcttaa tgacattacc
    13081 tagtcgctga cgtgtataag gtgacaatct aacactagga tcagcatccg gatcatttaa
    13141 accggttgtg ctagtataaa gtgcgatgtc agagccattt tttgcaagtt gatatgcagc
    13201 atcaaagcca ctttcattac ctccgataac cacatattgc cccttattaa agttatcaaa
    13261 gtcttcaatt tcactataat gaataccata tttaaatggc tttttaggga aattataatc
    13321 acctgttgcg acaaagatat aatccgcgtg atatgtctct gttgtcgttg caatcgtata
    13381 atatgcatca tctgcagata tatttgtgac aactgtattt tcaaagatat tcagctcgta
    13441 atggttggca accacttgta aatattcagc atatgtttct ccggaaatat gttcttcatt
    13501 aaatgtaaat gctggtgaag tatccatgga aattgcattc atatcaggca tgccaaatcc
    13561 attagacgta aatgatggcg tgatcgtacg ggtcgatttc ggccaatgtt taaatgaatg
    13621 tcctactgtt cctttttcta aaataatgac atctgttata ccgaaatctt ttaaggtaat
    13681 ggccatacct atacctgcag caccggcacc gataatagcc actttatgat gttgcatatt
    13741 aacactccct caatgatcta tttcaagtta ttgtttttga ctataaatcg taataattac
    13801 gatttataaa gtatagacct atccttaaga aatttcaata ttaaaagaaa aacttccgtt
    13861 attgtagaga attcatctac atttcggaag ttggtatgtg tcttttttat taaatttaag
    13921 gatgctacgg ttagttcaaa tgattatgaa tgacatttaa tggtatcatc cagcctcagt
    13981 acttggagat aacattttgg cggtgagaca taaatcatat ttttacaaaa cttatttcgt
    14041 cgtctcccca ataaagataa agtatttctt tattgtggtg taacttctcc acgttgtatt
    14101 tcactatcca aattttgttt catattttca aacaactctt gaaatctaac ctcatcttga
    14161 tcagaataac ctttaaaaat aactgaatct atattttctt cgttaaaata ataaatttca
    14221 tcttccacaa caccaatcgg atatttacat gcagaatagt caaacatata cttttgattt
    14281 tcaatttcta caattggtcc tctattaata atcattaact tttgcgaacc ttcttttaaa
    14341 taaataatgc ttcctattgt ttccatttat tttcctcctt tgtcaccgtc tcaatttcaa
    14401 cattccttcg catttccaag tcaatcatct cttcttccgc tttacgaatg ccatgttgaa
    14461 tttgctgttg ctgctcgtat aacgcttccg ttgaacgtcg tttcgcaaaa tgaaattcat
    14521 cgcggaactg ttccatacga ctagccatat tgctactatg ataacggtca cctaaatgtt
    14581 gatataattg ttctatcgaa tgattaagct catcactttc gtctatcaaa gctttaacct
    14641 ctcgcttttc atcatataag tcatccattt gcttatgttt cgctgtgatc attttattga
    14701 ttccatcaag tttattcgac atcatttcac cacggtttaa tttttaccaa tttcattacc
    14761 aatcattacg tctgtttgat taaattcttc gccaactttt tgtatactat cgctcgcatt
    14821 tcttattgct tcagcaacag attgattctt actaatcaaa tcgtctatca cactatgagc
    14881 attattattt ccacttactg ttgtactttc atctttatcc ggtttcgaca cattattaca
    14941 tgtttcaacg gcacttttaa tagatgatga tgcactatta gtcttgaaaa tatcactttg
    15001 aactgtactc attatttaca cctcttccca tttaaattat tttaaaatta ttagaaactg
    15061 tatcaatata tgatattcaa acattcaata atacttaaat attgttttgt gattatattc
    15121 aaaattattg tatcaataaa tatgtatcat tcctagaatt ttattgaaca aaaagttata
    15181 aaatcacttt tattgcaact caaccttgtt ttgcattaca gaaattaaca atagcgatta
    15241 aaattttatt ttccagcaat aaaaaagcac aaatattttt attgaggcaa ctgaaggcct
    15301 tctcttcata aaatatctgt gcatattatc tatttatctc aatctaacgt cttaactaac
    15361 ttgttgtacc ttgtaaaatc ctatccatag aatgcgcaac actatcacgg tacatctcac
    15421 caaatttcaa taaatgcacc atcaataaat ataaacgata aaattcaagt ctataggatg
    15481 ctccttttgc gagcggataa tgtttattat acgcatcgta aaattcgctc gtaaaaccac
    15541 caaataccgt tgtaataccg atatcaaatt ctctgtcacc atataatggt gctggatcaa
    15601 ataatgcagg acgtccatct tttaaaaaca tataattacc gccccataaa tcaccatgta
    15661 ataaagacgg tttactttga tgcttttcta attcctccac aatttgactt cgcactttgt
    15721 catatacttt gatatcgttg gcatcccata gccctctatt tagcaactct tctttcaaat
    15781 gatccaaacg tttatcgaca aaaatggtac gccaatcatc ttgccaatga ttatcaaaag
    15841 aaatatcgcc accttcataa ggtaatgaga agccaaattt gccttcttct tgttgctgac
    15901 tgtgtaattg agctacgagt tgacctaatt gacgttgact ccctgaagct ccttcttcta
    15961 aatacgtcat cactaaatac gcatcaccgt taacttcgcc acttgcaatt actctaggtg
    16021 ccgtgatacc tgcacgttca aattcattta aacctgcaat ttctgcagca taaaatgatt
    16081 ctttacgtcc acgttggaca agtaagaaaa atgtatccgt atctgtttcg actcgatatg
    16141 cttcgtttac atcaccacca ctcactggtg aaatctcttt aatatctttt aaaggtaaat
    16201 gctctaacca ttgttcattc atacaaacac cttccaatct acttacttta tactataata
    16261 ttgcctaatt aggatgattt aatcttaaaa acgttattca aacttgcgtc aattttcaaa
    16321 actttttagt tttaactagg aaattatgta gggaaatgct agtctattaa aagaatatat
    16381 ttcacatata ttaagttgag gatagtaagc ctcaaatata atgaaatagg agcatactca
    16441 tgtacaaatt aattaaacct ttcttattca aaatcgaacc cgaaaaagca cacggactaa
    16501 ctatcgatgc attaaaaacg ttacaaaagt ttccggtttt attcccagtc gtcgataaac
    16561 tatttactta taagaatcca acgttatcac aaacgataca aggtaatacg tacgacaatc
    16621 caattggctt agcagctggt ttcgacaaat cttgcgaagt accaaaagca ttggaacacc
    16681 ttggattcgg tgctttagaa ttaggtggta tcacacctaa acctcaaccg ggtaaccctc
    16741 aaccacgcat gtttagatta ttagaagatg acgccttgat aaatcgaatg ggcttcaata
    16801 atattggtat gaacaaagca ctcagtcatt tgcgtaaaaa tgcttatcaa gtacctgttg
    16861 gtatcaatgt tggtgtgaat aaaatgacac cttatgaagc gcgttatcaa gattatataa
    16921 aagtcattga tacgtttaaa cacgacgttt catttttcac agtcaatatc agttctccaa
    16981 atactgaaaa ccttcaaaat ttccatgata aagatgaatt ttcaatgtta tgtcaagctt
    17041 tgacaacatt taaaaaacag cacgatgtaa cagtgccaat ttacttaaaa ctaacgtctg
    17101 atatggattt cgatggctta aaagcactat taccagcgat tactgagaca tttgacggta
    17161 tcatcttagc aaacacaacg cgacaacgag atggtttaac ttctgctaat aaagtcgaag
    17221 aaggcggttt gagtggtcgt ccattatttg aacgtaattt aaaattgatt aagtatgctt
    17281 atcagcaaac aaatggtgaa tttttaatta taggtacagg cggcgtattc agtactgaag
    17341 atgcaatcaa aatgatgcgt cacggtgcgt cacttattca aatttattca tcacttgtta
    17401 ttgaaggccc aggtttaact aagaaaatga acaaaggcat cgcacgttac ttaaaagatc
    17461 atcattttga caatgtcagt gatattatag gactagatgc ctaatccaac gtagtcaaat
    17521 aacactttaa aacttgctga atcgtcttat taaaattatg atataactca agaagatata
    17581 tttatcacca tttttcaaaa taatggctta aatatcacat atcattaaat atagcgtttt
    17641 ataacggagg cgtttgttat gactaaagaa acagataata aaaaaacagt agcgacttct
    17701 gacaacaaag acacacagca acaaaatcaa actgaagaag atgaacgtca aggtttgaat
    17761 ggttatcgta aaacagatct tgatttagaa attgagcaag agctacgtga aatgatgaaa
    17821 acaggagaaa atgaaacgaa aaacgactac aaaaagttta aagtgttttc acttatttca
    17881 acacttgtca ttgtcatttt agcaattata agatttgttc ataaaatgat ttaattagag
    17941 tgagacattg ttttatgtct caggatccag ttattcagta tagctacaat atttacgatt
    18001 atataaataa cccgagattt tagtatgatt catttcacta aaatctcggg tttctatttg
    18061 ataattttta atgggatatg gcatgtatac gagcctgcct tttatctcat ttccaatgat
    18121 taatctggat attgttctaa aaatgctttc gcttctttat taactgtttt aaaatcaata
    18181 ccttgttgca tcgctgcaaa gacacatcca caataacact gcctaaagat attatagtca
    18241 ttacacattt ctatggaacg ctcataacct ttacttttct taaaatcact tggcaaatag
    18301 ttcacatcgt atattttttg gacatccata ccaagttcat tgattaattg tgcgttcttt
    18361 ttaggtgata acgtgattgc actgccaaaa taatcatagc catgttctac agctgctttc
    18421 gctacaatat ccaaacgcat ttcaaaacaa gcggtacaac gtaagccgcc ttctttttca
    18481 tcagctaatt ctttatcctt caccattttc acaaacttat gcggttcata aggtgcttca
    18541 atatacttca cattcgcacc agtcttgcga ttaaaatctt ccacaaattg ttcttgcact
    18601 ttagcacgtc gtaaatattc atttttcgga tgaatatttg aattcgcgaa ataaattgca
    18661 atgtctgcat attgtgttaa aaactctaat gtatatgtac tacaaggtgc acaacaacta
    18721 tgcaataaga ttttaggtct gattgcttct ctttcccact gcccgattaa tttcttcaac
    18781 accttgtcat aattaatttt ttgatttttc attttactga taataggttc agcattaatc
    18841 atgatttaac ctcccacatt taatcattaa cttctattat atatgattca tattaaatgt
    18901 cagtcaaaaa agttagaaat tcattttaat gcatttatat ttcgaaagtc ccttatgtat
    18961 ctaaaagcat tttttaaact tgaattttaa aacactaaac aacacatacg tctctgtgtc
    19021 attttcattt tttgtatgtc atatatatgt ttacttcatt taaatcaatt tcatcttaca
    19081 atttatcgtg tattttacaa aagattgact tcaattcatc gtaaaagtta tacttttgcc
    19141 attttttaat gtaacatggt gttagtaata aaaatactac attgaggtgt tttacatgac
    19201 agcattattc ccttatatcg cttttgaaaa ttcaaaagaa gcccttgcat attacgaaga
    19261 agtatttggt gcaactgacg ttaaacgttt agaagttggc gaagaacaag cgtcacattt
    19321 tggtatgact aaggaagaag cgcaagaagc aactatgcat gctgaatttg aagtgcttgg
    19381 cgtaaaagtg ctatgttctg attcttttgg tcgcgctgac aaaattaata atggcatatc
    19441 attattaatt gattatgatg ttaacaataa ggaagatgct gataaagttg aagcattcta
    19501 tgagaaaatt aaagatcatt cttcaattga aatagaatta ccgtttgctg accaattctg
    19561 gggtggcaaa atgggcgtct ttaccgataa atacggtgtt cgttggatgt tacatggtca
    19621 agactatact gcaatccaac aataatgtta ttgataaaga tatcatttaa taagcttatg
    19681 ttatttagca aggcaagcca ctctcgattc atggcttgcc ttattttatt gtatattttt
    19741 aattatcgtt gctggccctt gattgacaat cgtattaaaa tgcattatta agtcaacttt
    19801 gtctatacgg tttggatctt ctacccaatg tcttatcaaa gacaatcccg cacctgaaac
    19861 ataactcatg aaataagaaa atggtatacc attaatttga tcattttcat ttttataacg
    19921 ttgtaaatga cacataatta agtcatatag tttttcgtgt atatttgaat ctttacccat
    19981 acgaaacatc aattgataaa atgcgatgtc tttttctatc atatctatta aaacagtcat
    20041 aatttgatgt atgttatccg tggataactt aactgctcca tttaacttct catcatgaat
    20101 gaagtctctt atttcctcca actgctggtc ctctaatttt tcaagcaaat catatttatc
    20161 ataataatgc gtataaaatg tactacggtt aacatcagct aaatctgcaa tttgttgcac
    20221 agtaatctct tctaattggt gttgatgtaa aagttcaata aatgcatttc tcattgcaac
    20281 ttgtgatttt ctaatacgtc gatctatagt catttatatc aagtcctccc caatgattat
    20341 aaacaattat aaacgttatg ttcattatcc cacaaatctc caacattgat gattggcaca
    20401 caatgtttac ctgtttaata taggtgatac aaacaaacag aaaaaggtga taacaatgaa
    20461 ccaacattta ctaggaaatc caaaattaac tgtaactcat gtcaatgaag tgaaaaccgg
    20521 tattaaccac atcgttgtcg acagtgttca atatggaaat caagaaatga ttatggaaaa
    20581 agatgtcact gtggaaatgc gcgatggcga aaaattatat attaatattt tcagaccaaa
    20641 taaagatggc aaattccctg tagttatgtc tgcagatact tacggtaaag ataataagcc
    20701 taaaatcacc aatatgggtg ccctttggcc aacattaggc gccattccga catctagttt
    20761 tacacctgaa gaatcaccag acccaggatt ttgggtacca aacgattatg ttgtagttaa
    20821 agttgcatta cgcggtagtg acaaatccaa aggcgtctta tctccatggt caaaaagaga
    20881 agcggaagat tattacgaag tgattgaatg ggcagcaaaa cagccatgga gtaatggaaa
    20941 tatcggaaca aatggtgttt cttatcttgc ggtgactcaa tggtgggtcg catcattaaa
    21001 tccaccacat ttaaaagcaa tgattccttg ggaaggatta aatgatatgt atagagaagt
    21061 agcctttcac gggggtattc ctgatactgg cttttatcgt ttctggatac aaggtatttt
    21121 tgcgagatgg acagataatc caaatatcga agacatgatt caagcacaac aagaacaccc
    21181 tttgttcgat gatttttgga aacagcgtca agtgccatta tcacaaatta aaacacctct
    21241 actaacatgt gctagttggt ctacacaagg tttgcacaac cgtggctctt ttgaaggatt
    21301 taaacaagct gcatcagaag aaaaatggct atatgtgcat ggacgtaaag agtgggaaag
    21361 ttactacgct agagaaaatc tcgaacgcca aaaatcattc tttgattttt accttaaaga
    21421 agaaaataac gattggaaag atacgcctca tgtcatatat gaagttagag atcaatttta
    21481 taaaggcgaa ttcaaatcag cgtcagcgtt ccctttacct aacacagaat atacaccatt
    21541 gtatttaaat gctgaaaatc acacattgaa tcatgcaaag attagtagcg cgtatgtcgc
    21601 acaatatgac tctgaagata aacgacaaga tgtaagtttt aaatatacgt ttgacaaaga
    21661 tactgagtta gttggaaaca tgaacttaaa actatgggta agcactacag actcagacga
    21721 tatggattta tttgcaggta ttaaaaagtt agatcgtcgt ggtaatgaag ttaacttccc
    21781 tgattttaat catattgaaa atggtcaagt agctactggt tggttacgcg tatcacatcg
    21841 tgaattagat caagaaaaat ccacaatcgc gcaaccttgg cataaacatg aaacagaatt
    21901 aaagttgtca caagatgaga ttgtacctgt tgaaatcgaa ttgttacctt caggcacgct
    21961 atttaaacaa ggcgaaacat tggaagttgt tgtaaaaggt agtgaaattg taattggtaa
    22021 tagtacccct ggcatgaaaa cacgttatga acatgaagaa accataaata acggcatgca
    22081 catgatttat actggtggta aatatgattc acaattaatc attcctatcg ttaattgaca
    22141 tgcaggaatt acggtcgatt ttgattaaaa gtgacatagt gatagaagag aaggccacac
    22201 gcttggaatc ttaaaccaag gtgtggccct ttttattatt gatggctatt taattttata
    22261 aatcttgtca ctaaacttac tacaagcaat attaaaccaa taaataaagc cattattgct
    22321 gtattacttt cgttgtttac tcctgttttt ggcaattctt tcaattgttg cttatgtact
    22381 gacttgctct tgctatccaa atgaatactt tgttgtttag ccatttgttt attacttttt
    22441 gtgtgaatat catttgcttt aatacttgga tttacagcac tattattcga tgttccattt
    22501 gcgtttttca actgattaat aacatttgga tctttcgtgc catctattaa attatctgga
    22561 tgttttagta aatgtctgca gtcggatact attttagaat cttttgcctt taccgcatta
    22621 ttcggcacct cattatcatc tttcaactgc ttgataacat ctggatcttt cgtgccatct
    22681 attaaattat caagtttttt cacacgtgct atgtcactaa ctggccctgg ataatttgca
    22741 tccggttgtc taactggtat taaattgctg ttttcattca caccttcagg tttgttattt
    22801 tcggcgttcg cattatccgt aaaaatcaac actgataaaa ttgcaacaaa taaacttata
    22861 acttttttca tcttattcat cctttcctat tttactacct taatatcatt gctattaaag
    22921 taacccatcc atctattttt ttcgtcgtat aaagagtaat atttataccc atttggaata
    22981 ttgtaataga gtttagctgt gtacgcttta tcttttttaa ttaatttatt atcacccttt
    23041 tttgtaattt caatgttttt ccaatatata tacttacttt taattacttt cactttctta
    23101 ttatatggat gacttctata cttacctatc caataattta tgtaatctaa ttttaaatca
    23161 tttattctca cgcctgaatt atgattatat ctgccgtatg catgtactcc gatgatattg
    23221 ttttttgaat ctcttatcgc actaccactt tgtcctgaaa atgtgtctac cgtataatac
    23281 atttcattct catcaaaact ttctaatttc cctttacctt tatattgtct gttgtctgac
    23341 ttgtctcctg gaaatccaga aatttcaata ttttcacctt tgctaatgtg tgtgttcaaa
    23401 gttaagtaac ctgttttatt tcctaaatta ctatttaact taataattgc tatatcttct
    23461 tttgtaggag cattattaat ccaagtttta gatacaaaaa acttatgcga aaaggccttt
    23521 cctattgtat atgtttgccc atttactcca gcatagacat caatcttttt agcccaaccc
    23581 ttatcgtctt tagaatagac attatgagcc gctgtcaata caatgtcctt accaatcata
    23641 gtaccggtgc ctttatatac acggttattt gaaaacgtca tatttaataa tacagtctgc
    23701 ttataaggtc tttgtaaaat attattaaca accgttcttt gatctttacc gaaaactcgc
    23761 tcagttaagg gcgtactata atcacctact tcataaagct cattttgatt acgttcaatt
    23821 gttcctgatt caaaattatt atcactacca ttttctgaat tgacgatagg attattatca
    23881 gctaagacaa aactttcacc ataataacta gaaacaatta ctactaacaa aatcaaaaat
    23941 gatattttaa aaatttttgt cataggcatt cctcctttaa tctatattat ttttatctat
    24001 ttcatataat tttattatat aattgctatc aattcaa
//
LOCUS       DAOBBH010000022        23224 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.022, whole genome shotgun sequence.
ACCESSION   DAOBBH010000022 DAOBBH010000000
VERSION     DAOBBH010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 23224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23224
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.022"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            159..290
                     /locus_tag="P6L01_000611"
     CDS             159..290
                     /locus_tag="P6L01_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029699.1"
                     /translation="MYGAYLINEYHQDFKSIANFKVLKRMINVMMCYCVLFLEPRIS"
     gene            complement(407..1270)
                     /locus_tag="P6L01_000612"
     CDS             complement(407..1270)
                     /locus_tag="P6L01_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter"
                     /protein_id="HDP3029700.1"
                     /translation="MQFLDFLIALLPALFWGSVVLINVFVDGGPYNQIRGTTLGALIV
                     GLGLLITGFAKFNNPTVIIVGLISGALWAFGQANQLKSISLIGVSNTMPVSTGMQLVG
                     TTLFSVIFLGEWSSMTQIIFGLIAMILLVTGVALTSLKAKNERQSDNPEFKKAMGILI
                     VSTVGYVGFVVLGDIFGVGGTDALFFQSVGMAIGGFILSMNHKTSLKSTALNLLPGVI
                     WGIGNLFMFYSQPKVGVATSFSLSQLLVIVSTLGGIFILGERKDRRQMTGIWAGIIII
                     VIAAIILGNLK"
     gene            complement(1451..2338)
                     /locus_tag="P6L01_000613"
     CDS             complement(1451..2338)
                     /locus_tag="P6L01_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDP3029701.1"
                     /translation="MSIVQLYDITQIKSFIEHSNYESASYLYKLPQQYNEIDVLITDA
                     IESPGVFSIKENDSIKAIILSFAYDKNKFKVIGPFVADNYVLSVDTFETLFKAMTSNQ
                     PDDAVFNFSFEEGIQQYKPLMKVIQASYNFTDYYIEARTRLEEDMHQPNIIPYHKGFY
                     RAFSKLHTTTFKYQAQSPQDIIDSLDDHHHLFLFVSEGLLKGYLYLEIDSQQSIAEIK
                     YFSSHVDYRLKGIAFELLAYALQYAFDNFDIRKVYFKIRNKNNKLIERFNGLGFHINY
                     EYIKFKFESRNVKDQTIPE"
     gene            2404..2487
                     /locus_tag="P6L01_000614"
     CDS             2404..2487
                     /locus_tag="P6L01_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029702.1"
                     /translation="MILTCYNTFNTIEFKSKISGIDEARSK"
     gene            2513..4648
                     /locus_tag="P6L01_000615"
     CDS             2513..4648
                     /locus_tag="P6L01_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDP3029703.1"
                     /translation="MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHL
                     VTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIA
                     TDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
                     ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSL
                     TVKGFDFQLESNQRYTNKETLEQMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQ
                     DMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQATMAATYK
                     DQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLE
                     ALMPPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKI
                     SKTNIREHETTPPEYFNEGSLLKAMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADI
                     IDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAK
                     TFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPS
                     CKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKV
                     SRKEMKKYMNKNEGLDNNPFKDALKNLNL"
     gene            4762..6096
                     /locus_tag="P6L01_000616"
     CDS             4762..6096
                     /locus_tag="P6L01_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDP3029704.1"
                     /translation="MKNYFQFDKYGTNFKREILGGITTFLSMAYILAVNPQVLSLAGV
                     KGVSEDMKMDQGAIFVATALAAFVGSLFMGLIAKYPIALAPGMGLNAFFAFTVVLTMG
                     IPWQVGLTGVLFSGIFFAILTVTGFREVIINAIPYQMKMAVSAGIGLFITFVGLQSSG
                     IIVKNESTLVTLGHLTDPPVLLAIFGIVITVILYAIKLPGSIFIGMIITAIVGMFTGL
                     IQMPSGIVGKIPSIEPTFGAAFEAFKDPSQLLTIQFLIVILTFLFIDFFDTAGTLVAV
                     ATQAGIMKDNKLPRAGRALFSDSLATIVGSVFGTTTTTSYIESTSGVAVGARTGFASI
                     VTGCCFLLALFFSPLIAVVTSAVTTPALVVVGVLMAANFAEINWKSFEVAVPAFITII
                     MMPLSYSIATGIACGFIFYPITMLISKKHKEVHPIMYVLMVLFILYFIFVHG"
     gene            complement(6287..6676)
                     /locus_tag="P6L01_000617"
     CDS             complement(6287..6676)
                     /locus_tag="P6L01_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029705.1"
                     /translation="MKNGPKLSLALIGIFLILCEFFYGIPFLGATFILSFGWQPLLFN
                     ALLYLILTIILLVNRQNAIRPIAIIPIFGIVGSFLAIIPFLGILIHWILFFLMILFVL
                     VVLSAPTYIPNKNARVIYTQYKDGHRE"
     gene            7033..7341
                     /gene="rpsJ"
                     /locus_tag="P6L01_000618"
     CDS             7033..7341
                     /gene="rpsJ"
                     /locus_tag="P6L01_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDP3029706.1"
                     /translation="MAKQKIRIRLKAYDHRVIDQSAEKIVETAKRSGADVSGPIPLPT
                     EKSVYTIIRAVHKYKDSREQFEQRTHKRLIDIVNPTPKTVDALMGLNLPSGVDIEIKL
                     "
     gene            7369..8031
                     /gene="rplC"
                     /locus_tag="P6L01_000619"
     CDS             7369..8031
                     /gene="rplC"
                     /locus_tag="P6L01_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDP3029707.1"
                     /translation="MTKGILGRKIGMTQVFGENGELIPVTVVEAKENVVLQKKTVEVD
                     GYNAIQVGFEDKKAYKKDAKSNKYANKPAEGHAKKADAAPKRFIREFRNVDVDAYEVG
                     QEVSVDTFVAGDVIDVTGVSKGKGFQGAIKRHGQSRGPMSHGSHFHRAPGSVGMASDA
                     SRVFKGQKMPGRMGGNTVTVQNLEVVQVDTENKVILVKGNVPGPKKGLVEIRTSIKKG
                     NK"
     gene            8058..8681
                     /gene="rplD"
                     /locus_tag="P6L01_000620"
     CDS             8058..8681
                     /gene="rplD"
                     /locus_tag="P6L01_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDP3029708.1"
                     /translation="MANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASL
                     RQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMP
                     KKMRRLALRSALSFKAQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENED
                     VNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLG"
     gene            8681..8956
                     /gene="rplW"
                     /locus_tag="P6L01_000621"
     CDS             8681..8956
                     /gene="rplW"
                     /locus_tag="P6L01_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDP3029709.1"
                     /translation="MEARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEE
                     IFNVKVASVNIMNYKPKKKRMGRYQGYTNKRRKAIVTLKEGSIDLFN"
     gene            8989..9822
                     /gene="rplB"
                     /locus_tag="P6L01_000622"
     CDS             8989..9822
                     /gene="rplB"
                     /locus_tag="P6L01_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDP3029710.1"
                     /translation="MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRN
                     NQGKLTVRHHGSGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKR
                     YIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGA
                     SAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKK
                     K"
     gene            9889..10167
                     /gene="rpsS"
                     /locus_tag="P6L01_000623"
     CDS             9889..10167
                     /gene="rpsS"
                     /locus_tag="P6L01_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDP3029711.1"
                     /translation="MARSIKKGPFVDEHLMKKVEAQEGSEKKQVIKTWSRRSTIFPNF
                     IGHTFAVYDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHVADDKKTRR"
     gene            10196..10549
                     /gene="rplV"
                     /locus_tag="P6L01_000624"
     CDS             10196..10549
                     /gene="rplV"
                     /locus_tag="P6L01_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDP3029712.1"
                     /translation="MEAKAVARTIRIAPRKVRLVLDLIRGKNAAEAIAILKLTNKASS
                     PVIEKVLMSALANAEHNYDMNTDELVVKEAYANEGPTLKRFRPRAQGRASAINKRTSH
                     ITIVVSDGKEEAKEA"
     gene            10573..11226
                     /gene="rpsC"
                     /locus_tag="P6L01_000625"
     CDS             10573..11226
                     /gene="rpsC"
                     /locus_tag="P6L01_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDP3029713.1"
                     /translation="MGQKINPIGLRVGIIRDWEAKWYAEKDFASLLHEDLKIRKFIDN
                     ELKEASVSHVEIERAANRINIAIHTGKPGMVIGKGGSEIEKLRNKLNALTDKKVHINV
                     IEIKKVDLDARLVAENIARQLENRASFRRVQKQAITRAMKLGAKGIKTQVSGRLGGAD
                     IARAEQYSEGTVPLHTLRADIDYAHAEADTTYGKLGVKVWIYRGEVLPTKNTSGGGK"
     gene            11229..11663
                     /gene="rplP"
                     /locus_tag="P6L01_000626"
     CDS             11229..11663
                     /gene="rplP"
                     /locus_tag="P6L01_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDP3029714.1"
                     /translation="MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITS
                     RQIESARIAMTRYMKRGGKVWIKIFPHTPYTKKPLEVRMGAGKGAVEGWIAVVKPGRI
                     LFEVAGVSEEVAREALRLASHKLPVKTKFVKREELGGETNES"
     gene            11653..11862
                     /gene="rpmC"
                     /locus_tag="P6L01_000627"
     CDS             11653..11862
                     /gene="rpmC"
                     /locus_tag="P6L01_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDP3029715.1"
                     /translation="MKAKEIRDLTTSEIEEQIKSSKEELFNLRFQLATGQLEETARIR
                     TVRKTIARLKTVAREREIEQSKANQ"
     gene            11886..12149
                     /gene="rpsQ"
                     /locus_tag="P6L01_000628"
     CDS             11886..12149
                     /gene="rpsQ"
                     /locus_tag="P6L01_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDP3029716.1"
                     /translation="MSERNDRKVYVGKVVSDKMDKTITVLVETYKTHKLYGKRVKYSK
                     KYKTHDENNSAKLGDIVKIQETRPLSATKRFRLVEIVEESVII"
     gene            12181..12549
                     /gene="rplN"
                     /locus_tag="P6L01_000629"
     CDS             12181..12549
                     /gene="rplN"
                     /locus_tag="P6L01_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDP3029717.1"
                     /translation="MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVK
                     NATPGGVVKKGDVVKAVIVRTKSGVRRNDGSYIKFDENACVIIRDDKGPRGTRIFGPV
                     ARELREGNFMKIVSLAPEVL"
     gene            12585..12902
                     /gene="rplX"
                     /locus_tag="P6L01_000630"
     CDS             12585..12902
                     /gene="rplX"
                     /locus_tag="P6L01_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDP3029718.1"
                     /translation="MHIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKH
                     QKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEIK
                     SNN"
     gene            12929..13468
                     /gene="rplE"
                     /locus_tag="P6L01_000631"
     CDS             12929..13468
                     /gene="rplE"
                     /locus_tag="P6L01_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDP3029719.1"
                     /translation="MNRLKEKFNTEVTENLMKKFNYSSVMEVPKIDKIVVNMGVGDAV
                     QNSKVLDNAVEELELITGQKPLVTKAKKSIATFRLREGMPIGAKVTLRGERMYEFLDK
                     LISVSLPRVRDFQGVSKKAFDGRGNYTLGVKEQLIFPEIDYDKVSKVRGMDIVIVTTA
                     NTDEEARELLANFGMPFRK"
     gene            13491..13676
                     /locus_tag="P6L01_000632"
     CDS             13491..13676
                     /locus_tag="P6L01_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDP3029720.1"
                     /translation="MAKTSMVAKQQKKQKYAVREYTRCERCGRPHSVYRKFKLCRICF
                     RELAYKGQIPGVRKASW"
     gene            13708..14106
                     /gene="rpsH"
                     /locus_tag="P6L01_000633"
     CDS             13708..14106
                     /gene="rpsH"
                     /locus_tag="P6L01_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDP3029721.1"
                     /translation="MTMTDPIADMLTRVRNANMVRHEKLELPASNIKKEIAEILKSEG
                     FIKNVEYVEDDKQGVLRLFLKYGQNDERVITGLKRISKPGLRVYAKASEMPKVLNGLG
                     IALVSTSEGVITDKEARKRNVGGEIIAYVW"
     gene            14131..14667
                     /gene="rplF"
                     /locus_tag="P6L01_000634"
     CDS             14131..14667
                     /gene="rplF"
                     /locus_tag="P6L01_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDP3029722.1"
                     /translation="MSRVGKKIIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFK
                     QEENTIEVVRPSDSKEDRTNHGTTRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKD
                     LILNVGYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGK
                     GIRYQGEYVRRKEGKTGK"
     gene            14698..15057
                     /gene="rplR"
                     /locus_tag="P6L01_000635"
     CDS             14698..15057
                     /gene="rplR"
                     /locus_tag="P6L01_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDP3029723.1"
                     /translation="MISKIDKNKVRLKRHARVRTNLSGTAEKPRLNVYRSNKHIYAQI
                     IDDNKGVTLAQASSKDSDIATTATKVELATKVGEAIAKKAADKGIKEIVFDRGGYLYH
                     GRVKALAEAARESGLEF"
     gene            15078..15578
                     /gene="rpsE"
                     /locus_tag="P6L01_000636"
     CDS             15078..15578
                     /gene="rpsE"
                     /locus_tag="P6L01_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDP3029724.1"
                     /translation="MARREEETKEFEERVVTINRVAKVVKGGRRFRFTALVVVGDKNG
                     RVGFGTGKAQEVPEAIKKAVEAAKKDLVVVPRVEGTTPHTITGRYGSGSVFMKPAAPG
                     TGVIAGGPVRAVLELAGITDILSKSLGSNTPINMVRATIDGLQNLKNAEDVAKLRGKT
                     VEELYN"
     gene            15595..15774
                     /gene="rpmD"
                     /locus_tag="P6L01_000637"
     CDS             15595..15774
                     /gene="rpmD"
                     /locus_tag="P6L01_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDP3029725.1"
                     /translation="MAKLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIR
                     GQINKVKHLVTVEEK"
     gene            15791..16231
                     /gene="rplO"
                     /locus_tag="P6L01_000638"
     CDS             15791..16231
                     /gene="rplO"
                     /locus_tag="P6L01_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDP3029726.1"
                     /translation="MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGG
                     GVRPGFEGGQLPLFRRLPKRGFTNINRKEYAIVNLDQLNKFEDGTEVTPALLVESGVV
                     KNEKSGIKILGNGSLDKKLTVKAHKFSASAAEAIDAKGGAHEVI"
     gene            16231..17523
                     /gene="secY"
                     /locus_tag="P6L01_000639"
     CDS             16231..17523
                     /gene="secY"
                     /locus_tag="P6L01_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDP3029727.1"
                     /translation="MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAF
                     DNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQ
                     GEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAG
                     TAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVL
                     GLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFA
                     MAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEK
                     MADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQ
                     SIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR"
     gene            17540..18187
                     /locus_tag="P6L01_000640"
     CDS             17540..18187
                     /locus_tag="P6L01_000640"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDP3029728.1"
                     /translation="MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETE
                     LGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSE
                     LDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQR
                     EDDNPETVANRLSVNIKQSKPILDFYVQKGVLKNIDGSKDISDVTKDVIDILDHL"
     gene            18380..18598
                     /gene="infA"
                     /locus_tag="P6L01_000641"
     CDS             18380..18598
                     /gene="infA"
                     /locus_tag="P6L01_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDP3029729.1"
                     /translation="MAKQDVIELEGTVLDTLPNAMFKVELENGHEILAHVSGKIRMNY
                     IRILPGDKVTVEMSPYDLTRGRITYRYK"
     gene            18630..18743
                     /gene="rpmJ"
                     /locus_tag="P6L01_000642"
     CDS             18630..18743
                     /gene="rpmJ"
                     /locus_tag="P6L01_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDP3029730.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQRQG"
     gene            18766..19131
                     /gene="rpsM"
                     /locus_tag="P6L01_000643"
     CDS             18766..19131
                     /gene="rpsM"
                     /locus_tag="P6L01_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDP3029731.1"
                     /translation="MARIAGVDIPREKRVVISLTYIYGIGTSTAQKILEEANVSADTR
                     VKDLTDDELGRIREVVDGYKVEGDLRRETNLNIKRLMEISSYRGIRHRRGLPVRGQKT
                     KNNARTRKGPVKTVANKKK"
     gene            19155..19544
                     /gene="rpsK"
                     /locus_tag="P6L01_000644"
     CDS             19155..19544
                     /gene="rpsK"
                     /locus_tag="P6L01_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDP3029732.1"
                     /translation="MARKQVSRKRRVKKNIENGVAHIRSTFNNTIVTITDEFGNALSW
                     SSAGALGFKGSKKSTPFAAQMASETASKSAMEHGLKTVEVTVKGPGPGRESAIRALQS
                     AGLEVTAIRDVTPVPHNGCRPPKRRRV"
     gene            19619..20563
                     /locus_tag="P6L01_000645"
     CDS             19619..20563
                     /locus_tag="P6L01_000645"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDP3029733.1"
                     /translation="MIEIEKPRIETIEISEDAKFGKFVVEPLERGYGTTLGNSLRRIL
                     LSSLPGAAVKYIEIEGVLHEFSAVDNVVEDVSTIIMNIKQLALKIYSEEDKTLEIDVR
                     DEGEVTASDITHDSDVEILNPELKIATVSKGGHLKIRLVANKGRGYALAEQNNTSDLP
                     IGVIPVDSLYSPVERVNYTVENTRVGQSSDFDKLTLDVWTNGSITPQESVSLAAKIMT
                     EHLNIFVGLTDEAQNAEIMIEKEEDQKEKVLEMSIEELDLSVRSYNCLKRAGINSVQE
                     LADKSEADMMKVRNLGRKSLEEVKYKLEDLGLGLRKED"
     gene            20580..20948
                     /gene="rplQ"
                     /locus_tag="P6L01_000646"
     CDS             20580..20948
                     /gene="rplQ"
                     /locus_tag="P6L01_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDP3029734.1"
                     /translation="MGYRKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVV
                     EKLITLGKKGDLASRRNAAKTLRNVEILNEDETTQTALQKLFGEIAERYTERQGGYTR
                     ILKQGPRRGDGAESVIIELV"
     gene            21486..22295
                     /locus_tag="P6L01_000647"
     CDS             21486..22295
                     /locus_tag="P6L01_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDP3029735.1"
                     /translation="MEDKNSVIVFKNVSFQYQSDASFTLKDVSFNIPKGQWTSIVGHN
                     GSGKSTIAKLMIGIEKVKSGEIFYNNQAITDDNFEKLRKDIGIVFQNPDNQFVGSIVK
                     YDVAFGLENHAVPHDEMHRRVSEALKQVDMLERADYEPNALSGGQKQRVAIASVLALN
                     PSVIILDEATSMLDPDARQNLLDLVRKVKSEHNITIISITHDLSEAMEADHVIVMNKG
                     TVYKEGTATEIFDHAEELTRIGLDLPFPIKINQMLGHQTSFLTYEGLVDQL"
     gene            22292..23152
                     /locus_tag="P6L01_000648"
     CDS             22292..23152
                     /locus_tag="P6L01_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDP3029736.1"
                     /translation="MTIRFDNVSYTYQKGTPYQHQAIHDVNTEFEQGKYYAIVGQTGS
                     GKSTLIQNINALLKPTTGTVTVDDITITHKTKDKYIRPVRKRIGMVFQFPESQLFEDT
                     VEREMIFGPKNFKMNLDEAKNYAHRLLMDLGFSRDVMSQSPFQMSGGQMRKIAIVSIL
                     AMNPDIIVVDEPTAGLDPQSKRQVMRLLKSLQTDENKAIILISHDMNEVARYADEVIV
                     MKEGSIVSQTSPKELFKDKKKLADWHIGLPEIVQLQYDFEQKYQTKLKDIALTEEAFV
                     SLYKEWQHEK"
     gene            23142..>23224
                     /locus_tag="P6L01_000649"
     CDS             23142..>23224
                     /locus_tag="P6L01_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDP3029737.1"
                     /translation="MKNKLIIGRYLPINSFVHHLDPRAKLM"
BASE COUNT         8383 a         3683 c         4485 g         6673 t
ORIGIN      
        1 aagtcagctt acaataatgt gcaagttggg gaggggcccc aacaaagaga aattggattc
       61 ccaatttcta cagacaatgc aagttggggt gggacgacga aatatatttt gcgaaaatat
      121 catttctgtc ccactcccta tgcagctatc cacttcatat gtatggcgca tacttgataa
      181 atgaatatca tcaagatttt aaaagtatcg ctaattttaa agtcttaaaa cgcatgatta
      241 atgtcatgat gtgttattgc gttttgtttt tagagccacg tatttcctag gacatataga
      301 ggaacattca acgtaaaatt cccgaaaatt aattttaatc attctataag tgaatggtta
      361 ttttcagaag tgattacatt tttacgttac atgagtattt aactttctat ttcaaattac
      421 ctagaattat agcagctatc acgataataa taatacctgc ccaaataccc gtcatctgac
      481 gacgatcttt tctttctcct aaaatgaaaa taccacctaa tgttgaaacg ataacaagta
      541 actgtgataa tgagaaactt gtagctacac caacttttgg ttgagaatag aacatgaaca
      601 agttaccaat tccccaaatc acacctggca atagattaag tgctgttgat ttaagtgatg
      661 ttttatgatt catggatagg ataaagccac caattgccat accgacagat tggaagaaca
      721 atgcatcagt tccaccaaca ccaaagatgt caccaagtac aacgaaacct acatatccaa
      781 ctgtagatac aattaaaata cccattgctt ttttaaattc aggattatct gattgacgtt
      841 catttttggc tttaagtgaa gtaagtgcta caccagtaac taataatatc atggcgatta
      901 aaccaaagat aatttgagtc attgaagacc attcacctaa aaagataacg ctgaataatg
      961 ttgtaccaac taattgcata cctgtagaaa ctggcatagt atttgataca ccgattaaac
     1021 taatagattt aagctgattc gcttgtccaa acgcccataa tgcaccagaa ataagaccga
     1081 caataattac agtaggatta ttgaattttg cgaaaccagt aataagtaaa cctaatccaa
     1141 caatgagtgc gcctagcgtc gtaccacgaa tttggttgta aggtccaccg tcgacgaaca
     1201 cattaataag aacgacactt ccccagaata aagcaggtaa aagtgcgatt aagaaatcaa
     1261 gaaattgcaa gactactcac tctcctatac tatttttgca agtgctcccc ttaattgaca
     1321 cttgcatgtt attgtaaagc attctgccct aatagtaaaa aacaactact tataattttc
     1381 aatatttaca aaaagtagaa aaacccacaa tacatgctaa tgaagtactg tggggtattt
     1441 gcttggtatt ttattcaggg attgtttgat cttttacgtt acgtgattcg aatttgaatt
     1501 taatgtactc atagttgata tggaaaccta gaccattaaa tcgttcgatg agtttattat
     1561 ttttattacg aattttaaaa taaacttttc taatatcaaa attatcaaaa gcatattgca
     1621 atgcatacgc aagcaactcg aaagcgatac ctttcaaacg gtaatctaca tgagaactga
     1681 agtatttaat ctcggcgatt gactgttgtg aatcaatttc aaggtataaa taacctttaa
     1741 gtaaaccttc gctaacaaat aaaaacaaat gatgatggtc gtctaaacta tcaatgatat
     1801 cttgcggtga ctgtgcctga tatttaaatg tagttgtgtg taatttgctg aaagcacgat
     1861 aaaacccttt gtgataagga atgatatttg gttggtgcat atcttcttct aatcttgtac
     1921 gggcttctat gtaatagtca gtgaagttat aacttgcttg aataactttc attaatggtt
     1981 tgtattgttg aatgccttct tcaaaagaaa agttaaagac ggcatcgtca ggttggttcg
     2041 aagtcattgc tttaaatagc gtttcaaacg tatcgacaga taatacataa ttgtcagcca
     2101 cgaatgggcc tatgactttg aatttatttt tatcgtatgc aaaagacaat atgattgctt
     2161 tgattgaatc gttttcttta atcgaaaata caccaggtga ttcaatcgca tcggttatta
     2221 atacatctat ttcattgtac tgttgaggaa gtttatataa gtatgatgct gattcataat
     2281 tcgaatgttc aatgaacgat tttatttgtg taatatcata taactgaact atgctcatgt
     2341 cattacctcc gtactttttg ttacttttat tatatagtat ttcaactgaa atgaaagtta
     2401 atagtgatat taacatgtta caatacattt aacaccattg aatttaaatc aaagattagt
     2461 ggaatagatg aagcacgttc gaaataaaag aacgtatgag aaaggataat ttatgaaatc
     2521 tttaatatta gctgaaaaac catcagttgc aagagatatt gctgatgctt tacaaataaa
     2581 tcagaagcgt aatggttact ttgaaaataa ccaatatatt gtcacgtggg cgttaggtca
     2641 tctagtgaca aatgcgacac ctgaacaata cgataaaaat ttaaaggaat ggcgattaga
     2701 agaccttcca attataccta aatatatgaa aactgttgtt attggtaaaa caagcaaaca
     2761 atttaaaaca gtaaaagcgt taattttaga taataaagtg aaagatatta ttattgcaac
     2821 agatgctgga cgagaaggtg aactagttgc aagattgatt ttggataaag ttggtaacaa
     2881 aaagccaatc cgtcgattat ggattagctc agttactaaa aaagctattc aacaaggttt
     2941 taaaaattta aaagacggtc gtcaatataa cgatttgtat tatgcagcgt tagcgagaag
     3001 cgaggcagat tggattgttg ggattaatgc aacgcgtgca ctaacaacaa agtatgatgc
     3061 acagctatcc ctgggacgtg ttcagacacc aacgattcaa ttagtaaata cacgacaaca
     3121 agagattaat cagttcaaac cacaacaata ctttacatta tcattaacgg taaaagggtt
     3181 tgattttcag ctagaatcaa atcagcgata taccaataaa gaaactttag aacagatggt
     3241 taataatttg aaaaatgtcg atggtaagat taaatctgtt gctactaaac ataagaagtc
     3301 gtatccgcaa tcactgtaca atttaacaga tttacaacaa gatatgtata gacgttataa
     3361 aattggacct aaagaaacat tgaatacact tcaaagctta tatgagagac ataaagtcgt
     3421 aacctatcca agaacagatt caaactattt aacaactgat atggtagata ctatgaaaga
     3481 acgtattcag gcgacgatgg cagcaacata taaagaccaa gcacgcccat taatgtctaa
     3541 aacattttca tcaaaaatgt cgatatttaa taatcaaaaa gtatctgatc accatgcaat
     3601 tattcctaca gaagtgagac ctgtcatgtc agacttaagt aatagagaat taaagttata
     3661 cgatatgatt gtcgagcgtt ttttagaagc tttaatgcct ccgcacgagt atgacgcgat
     3721 aactgtaact ttagaggttg cagggcacac atttgttttg aaagagaatg taacaactgt
     3781 tttaggtttt aaatctatta gacaaggtga atctattaca gagatgcaac agcctttttc
     3841 agaaggcgat gaagtgaaga tttcaaaaac aaacattaga gaacatgaaa caacacctcc
     3901 agaatatttt aatgaaggtt cgttattaaa agcgatggag aaccctcaga actttattca
     3961 attgaaggat aaaaaatatg cgcaaacttt aaaacaaaca ggtggtatcg gcacagttgc
     4021 aacaagggcc gacattatcg ataaattatt taatatgaat gccattgaat caagagacgg
     4081 taaaattaaa gtaacgtcaa aaggtaaaca aatattagaa ttagcaccag aagaattaac
     4141 gtcgccactt ttaactgcac aatgggaaga aaaattactt ttaattgaac gtggtaaata
     4201 tcaggcgaaa acatttatta atgaaatgaa agattttacg aaagatgttg taaatgggat
     4261 taaaaatagt gatcgtaaat ataaacacga taatttaaca accacagaat gcccaacgtg
     4321 tggtaaattc atgattaaag ttaaaactaa aaatggtcag atgcttgtgt gccaagatcc
     4381 atcttgtaag acgaaaaaga atgtacagcg caaaacaaat gcaagatgtc caaactgtaa
     4441 aaagaaatta acgttgtttg gtaaagggaa agaagcggta tatcgttgtg tttgtggaca
     4501 ttctgaaacg caagcacata tggatcagcg tatgaagtct aaatcctctg gtaaagtatc
     4561 tcgtaaagaa atgaaaaagt atatgaataa aaatgaaggt ttagacaata atccgtttaa
     4621 agatgcatta aagaacttga atttatagat aaaatcgaac aaagttgaat cagaaaaaac
     4681 gaaaagttcg cttttggtat tgttttttat taagaatgat attaaactat taaggtattt
     4741 taaaaaaagg agcatccatt cgtgaaaaac tatttccagt tcgataaata tggaacaaac
     4801 tttaaaagag aaatcttagg cggtatcaca actttcttat ctatggccta tattttagca
     4861 gttaacccgc aagttttaag tttagcaggt gttaaaggcg tatcagaaga tatgaaaatg
     4921 gaccaaggtg ccatttttgt agcgactgca ttagcagcat ttgtaggctc gctattcatg
     4981 ggactaatag ctaaatatcc aatcgcatta gcaccaggta tgggattaaa tgcattcttt
     5041 gcatttactg ttgtgttaac catgggtatt ccttggcaag ttggtttaac aggtgtttta
     5101 ttctcaggta tattctttgc gatattaacc gtcacagggt ttagggaagt tattattaat
     5161 gcaattcctt atcaaatgaa gatggctgtt tcagcaggta taggattatt cattacattt
     5221 gtaggtttac aaagttcggg tattatagtt aaaaatgaat ctacattagt aacattggga
     5281 catttaactg atccaccagt acttttggcg atattcggta tcgttattac agttatttta
     5341 tatgcaatta aattgccggg ttctatcttt atcggtatga tcatcacagc aatcgttggt
     5401 atgttcactg gattgattca aatgccatct ggtattgtag gtaagattcc aagtattgaa
     5461 ccaacgttcg gtgctgcatt tgaagcgttt aaagacccaa gtcaattatt aacgatacag
     5521 ttcttaattg ttattttaac gttcttattt attgatttct ttgatacagc tggtacatta
     5581 gtagctgttg ctactcaagc aggtattatg aaagataata aattaccaag agctggtaga
     5641 gcattgttct cagattcttt agctacgatt gtaggatcag tttttggaac aactacaaca
     5701 acttcttata tcgaatcaac ttcaggtgtt gctgtaggtg ctagaacagg atttgctagt
     5761 atcgtaacag gttgttgttt cctattagcg ctgttcttta gtccgttaat cgctgttgta
     5821 acaagtgcag ttacgacacc agctttagtg gtggtaggtg tattaatggc tgcaaacttt
     5881 gctgagataa actggaaaag ttttgaagtt gcagtgccag cattcattac aatcattatg
     5941 atgcctttat catactcaat tgcaactggt attgcgtgtg gatttatttt ctatccaatt
     6001 acaatgctta tctctaaaaa acataaagaa gtacatccga taatgtatgt attaatggta
     6061 ctattcattc tgtacttcat atttgttcat ggttaaatcc agacacaaaa aacgactgcc
     6121 aatgattggt agtcgttttt tctttgaagt aaggataaaa taagaagcga aataacataa
     6181 agcaatatta tgaatgtctt taaatatccg atataaatat acttctttta aaattgttgg
     6241 acgtgtttct tctatattat atacctaatt aaaaaggttt taagaattat tctctatgtc
     6301 catctttgta ttgtgtgtaa atgacacgag catttttatt aggtatatag gttggcgctg
     6361 ataatacaac taggacaaat agaatcatta ggaaaaataa aatccaatga atcaaaattc
     6421 ctaagaaggg gatgatagct aaaaaactac ctactatacc aaatattgga ataatcgcta
     6481 taggtctaat cgcattttgt ctattcacta ataatataat agtaagaatt agataaagta
     6541 atgcattaaa gagtaaaggc tgccatccaa aacttaaaat aaacgttgca cctaaaaaag
     6601 gaatgccata gaaaaactca caaagtatta aaaagatacc tataagtgct aaagataatt
     6661 ttggtccatt tttcatattc attcaccacc gttcttatga ctaattatat agaataattt
     6721 tcaatgataa taataaaatg attcaaggta cgaatttaca ataaagaagg aaataaaaaa
     6781 tatctcgaaa atagttgaac tgactaagta tataaagtaa aatataaaag tatgtgttag
     6841 acagatagaa aaattatgtg aaaagccttg atttatcgat gtttttcgtg taatataact
     6901 aacgttggac tttaagaaaa gcgatgaagc gaaaggttac tgacacaccc ggccgctttg
     6961 ccatggcgct gtgtaagata gttttcgtgg agaagtctat cactaaatgt agacgaataa
     7021 ggagggaaaa ttatggcaaa acaaaaaatc agaatcagat taaaagctta tgatcaccgc
     7081 gtgattgatc aatcagcaga gaagattgta gaaacagcga aacgttctgg tgcagatgtt
     7141 tctggaccaa ttccgttacc aactgagaaa tcagtttaca caatcatccg tgccgtgcat
     7201 aagtataaag attcacgtga acaattcgaa caacgtacac acaaacgttt aatcgatatt
     7261 gtaaacccaa caccaaaaac agttgacgct ttaatgggct taaacttacc atctggtgta
     7321 gacatcgaaa tcaaattata atagacaatt ttaggaggtg gactttcgat gaccaaagga
     7381 atcttaggaa gaaaaattgg gatgacacaa gtattcggag aaaacggtga attaatccct
     7441 gtaacagtag tagaagctaa agaaaatgtt gtattacaaa agaaaactgt agaagttgat
     7501 ggatacaacg caatccaagt tggatttgaa gacaaaaaag catacaaaaa agatgcaaaa
     7561 tctaataaat atgctaataa accagctgaa ggtcacgcta aaaaagctga cgcagcacct
     7621 aagcgcttca ttcgtgaatt ccgcaatgta gacgtggatg cttacgaagt aggtcaagaa
     7681 gtctcagtag atacttttgt agctggcgac gttattgacg taacaggcgt atcaaaaggt
     7741 aaaggtttcc aaggtgcaat taaacgccac ggacaatctc gtggacctat gtcacacggt
     7801 tctcatttcc acagagcacc aggttctgta ggtatggctt cagatgcttc tagagtattt
     7861 aaaggccaaa aaatgccagg acgtatgggt ggaaacactg taactgttca aaacttagaa
     7921 gtagttcaag ttgacacaga aaacaaagtt atcttagtaa aaggtaacgt acctggacct
     7981 aaaaaaggtt tagtagaaat cagaacttca attaaaaaag gtaataaata ataagaagtg
     8041 aaaggaggaa atgcataatg gctaattatg atgttttaaa attagacgga actaaatcag
     8101 gttcaatcga attaagcgat gcagtattcg gtattgagcc aaataatagc gttttattcg
     8161 aagctattaa tttacaacgt gcttcattac gtcaaggtac gcatgctgtt aagaatcgtt
     8221 cagcagtaag cggtggcgga cgtaaaccat ggaagcaaaa aggaacaggt cgtgctcgtc
     8281 aaggtacaat ccgtgctcca caatggcgtg gcggtggtat cgtattcgga ccaactccaa
     8341 gaagttatgc atacaaaatg cctaagaaaa tgcgtcgttt agctttacgc tcagcattat
     8401 ctttcaaagc tcaagagaat ggcttaactg tagttgacgc attcaacttc gaagctccaa
     8461 aaactaaaga attcaaaaat gtattatcta cattagaaca acctaaaaaa gtattagtag
     8521 ttactgaaaa cgaagatgta aatgttgaat tatcagcacg caacatccct ggcgttcaag
     8581 tgacaactgc tcaaggttta aatgttttag atatcactaa tgctgacagc ttagtaatta
     8641 ctgaagctgc tgctaaaaaa gttgaggagg tgctcggata atggaagcaa gagatattct
     8701 taagcgcccc gtaatcactg agaaatcttc tgaagcaatg gctgaagaca aatacacttt
     8761 cgacgttgat actcgtgtta acaaaacaca agtaaaaatg gcagttgaag aaatcttcaa
     8821 cgtaaaagtt gcaagtgtta atatcatgaa ttacaaacct aagaaaaaac gtatgggccg
     8881 ttaccaaggc tatacaaaca aaagaagaaa agcgattgta actcttaaag aaggatcaat
     8941 cgacttattt aactaataaa acaattacca ttaaggaggt aagcgacaat ggctattaaa
     9001 aagtataagc caataacaaa tggtcgtcgt aatatgactt cgttagattt cgcagaaatc
     9061 acgaaaacta cacctgaaaa gtcattatta aaaccgctac cgaaaaaagc gggacgtaac
     9121 aaccaaggta aattgactgt aagacaccat ggtagtggac acaaacgtca ataccgtgtt
     9181 atcgatttta aacgtaacaa agatggtatc aatgcaaaag ttgattctat tcaatatgat
     9241 ccaaaccgct cagcaaacat cgctttagtt gtatatgcag acggtgaaaa acgatatatc
     9301 attgctccta aaggattaga agtaggtcaa atcgttgaaa gtggtgctga agctgacatc
     9361 aaagttggta acgcattacc attacaaaac attccagttg gtacagtagt acacaacatc
     9421 gagcttaaac ctggtaaagg tggacaaatc gctcgttcag ctggtgcaag tgctcaagta
     9481 cttggtaaag aaggtaaata cgtattaatc agattaagat ctggtgaagt tcgtatgatc
     9541 ttatctactt gccgtgctac aatcggtcaa gttggtaacc tacaacacga attagttaac
     9601 gttggtaaag ccggacgttc aagatggaaa ggtatccgtc caacagttcg tggttctgta
     9661 atgaacccta acgatcaccc acacggtggt ggtgaaggtc gtgctcctat cggtagacca
     9721 tctccaatgt caccatgggg taaacctacg cttggtaaga aaactcgtcg tggtaaaaaa
     9781 tcatcagaca aacttatcgt tcgtggacgt aagaaaaaat aatataatca acttatttgg
     9841 gtgtgcggct taaagctgca cgcacataat aagaagggag gcgcccaaat ggctcgtagt
     9901 attaaaaaag gacctttcgt cgatgagcat ttaatgaaaa aagttgaagc tcaagaagga
     9961 agcgaaaaga aacaagtaat caaaacatgg tcacgtcgtt ctacaatttt ccctaatttc
    10021 atcggacata cttttgcagt atacgacgga cgtaaacacg tacctgtata tgtaactgaa
    10081 gatatggtag gtcataaatt aggtgagttt gctcctactc gtacattcaa aggacacgtt
    10141 gcagacgaca agaaaacaag aagataatat ctattaagta gaggaggaca tcctaatgga
    10201 agcaaaagcg gttgctagaa caataagaat cgcacctcgt aaagtaagac tagttcttga
    10261 cttaatcaga ggtaaaaatg ctgctgaagc tattgcaatt ttaaaattaa caaacaaagc
    10321 ttcatcacca gtaattgaaa aagtattaat gtccgcttta gctaatgctg aacataacta
    10381 tgacatgaac acagatgaat tagtagttaa agaagcatat gctaacgaag gaccaacatt
    10441 aaaacgtttc cgtccacgtg cgcaaggtcg tgcaagtgcg attaacaaac gtacaagcca
    10501 cattacaatc gtcgtaagtg acggtaaaga agaagctaaa gaagcttaat taacttttaa
    10561 ggagggaata ctgtgggtca aaaaattaat ccaatcggac ttcgtgttgg tattatccgt
    10621 gattgggaag ctaaatggta tgctgaaaaa gacttcgctt cacttttaca cgaagattta
    10681 aaaatccgta aatttattga taatgaatta aaagaagcat cagtttctca cgtagagatt
    10741 gaacgtgctg caaaccgtat caacattgca attcatactg gtaaacctgg tatggtaatt
    10801 ggtaaaggcg gttcagaaat cgaaaaatta cgcaacaaat taaatgcgtt aactgataaa
    10861 aaagtacaca tcaacgtaat tgaaatcaaa aaagttgatc ttgacgctcg tttagtagct
    10921 gaaaacatcg cacgtcaatt agaaaaccgt gcttcattcc gtcgtgtaca aaaacaagca
    10981 atcactagag ctatgaaact tggtgctaaa ggtatcaaaa ctcaagtatc tggtcgttta
    11041 ggcggagctg acatcgctcg tgctgaacaa tattcagaag gaactgttcc acttcatacg
    11101 ttacgtgctg acatcgatta tgcacacgct gaagctgaca ctacttacgg taaattaggc
    11161 gttaaagtat ggatctatcg tggagaagtt cttcctacta agaacactag tggaggagga
    11221 aaataataat gttactacca aaacgtgtaa aatatcgtcg tcaacatcgt cctaaaacaa
    11281 ctggtcgttc taaaggcggt aactacgtaa catttggtga gtttggttta caagctacaa
    11341 caacgtcttg gatcacatct cgtcaaatcg aatctgctcg tatagcaatg acacgttaca
    11401 tgaaacgtgg cgggaaagtt tggattaaaa tcttcccaca tacaccatat actaaaaaac
    11461 ctttagaagt acgtatgggt gctggtaaag gtgcggttga aggctggatc gcagttgtta
    11521 aaccaggtag aattttattc gaagttgctg gcgtttctga agaagttgcg cgtgaagcac
    11581 tacgtttagc aagtcacaaa cttccagtaa aaactaagtt tgtaaaacgt gaggaattgg
    11641 gtggtgaaac aaatgaaagc taaggaaatt agagacttaa ccacttcaga aatcgaagaa
    11701 caaatcaaat cttcaaaaga agagcttttt aacctacgct ttcagttagc tacaggtcaa
    11761 ttagaagaaa ctgcacgtat tcgtacagta agaaaaacga ttgcacgtct aaaaactgtt
    11821 gctcgtgaaa gagaaattga acaaagtaag gctaatcaat aattaagtaa gaggaggtta
    11881 caaaagtgag cgaaagaaac gatcgtaaag tttatgtagg taaagttgtt tcagacaaaa
    11941 tggacaagac tattacagta cttgttgaaa cttacaaaac acacaaatta tacggtaaac
    12001 gagtaaaata ctctaaaaaa tacaaaactc atgatgaaaa caattcagct aaattaggag
    12061 acattgttaa aattcaagaa actcgtcctt tatcagcaac aaaacgtttt cgtttagtag
    12121 agattgttga agagtcagta attatttaat acaagtttag agataaggga ggtttaacta
    12181 atgatccaac aagaaacacg cttgaaagta gcagacaact ctggtgctcg tgaagttctt
    12241 acaatcaaag tattaggtgg atctggtcgt aaaacagcaa acatcggcga tgttatcgta
    12301 tgtactgtta aaaatgcaac accaggtggc gttgttaaaa aaggtgacgt tgtcaaagct
    12361 gtaatcgtac gtactaagtc aggtgttcgt cgtaatgacg gttcatacat caaatttgat
    12421 gaaaatgcat gtgttatcat ccgtgatgac aaaggcccac gtggtactcg tatcttcgga
    12481 cctgttgctc gtgaattacg tgaaggtaac ttcatgaaaa tcgtatcatt agcaccagaa
    12541 gtactttaat taataacaac caatacaaat aaggaggtgc ccacatgcat atcaaaaaag
    12601 gtgacaacgt taaagttatc gcaggtaaag acaaaggtaa agaaggtaaa gtaattgcta
    12661 ctctacctaa aaaagaccgt gtcgttgtgg aaggtgttaa cattatgaaa aaacaccaaa
    12721 aaccaactca attaaatcct gaaggtggaa tcttagaaac agaggcagca atccatgttt
    12781 ctaatgtaca attattggac cctaaaacaa acgaaccaac tcgtgtaggt tacaaatttg
    12841 ttgatggtaa aaaagttcgt atcgctaaaa aatctggcga agaaattaaa tctaataatt
    12901 aataacaagt tgaaaggagg atcccacttt gaaccgttta aaagaaaagt ttaacactga
    12961 agttactgaa aacttaatga aaaaattcaa ttatagttca gtaatggaag taccaaaaat
    13021 agataaaatc gttgtgaaca tgggtgtagg tgacgcagta caaaattcta aagtattaga
    13081 caatgctgtt gaagaattag aattgatcac tggtcaaaaa ccattagtaa ctaaagctaa
    13141 aaaatcaatc gcgactttcc gtttacgtga aggtatgcca atcggtgcga aagtaacact
    13201 tcgcggtgaa agaatgtatg aattcttaga caaattaatt tcagtatcat taccacgtgt
    13261 acgtgacttc caaggtgttt ctaaaaaagc atttgacgga cgcggtaact acactttagg
    13321 tgttaaagaa caattaattt tcccagaaat cgactatgat aaagtaagta aagttagagg
    13381 aatggatatt gttatcgtaa cgactgctaa cactgatgaa gaagctcgtg aattgttagc
    13441 taacttcggt atgccattcc gtaaataatt atttaaagga ggctaattaa gtggctaaaa
    13501 cttcaatggt tgctaagcaa caaaaaaaac aaaaatatgc agttcgtgaa tacactcgtt
    13561 gtgaacgttg tggccgtcca cattctgtat atcgtaaatt taaattatgc cgtatttgtt
    13621 tccgtgaatt agcttacaaa ggccaaatcc ctggcgttcg taaagctagc tggtaataaa
    13681 aaagagtctg aaaggaggca acaatcaatg acaatgacag atccaatcgc agatatgctt
    13741 actcgtgtaa gaaacgcaaa catggtgcgt cacgagaagt tagaattacc tgcatcaaat
    13801 attaaaaaag aaattgctga aatcttaaag agtgaaggtt tcattaaaaa tgttgaatac
    13861 gtagaagatg ataaacaagg tgtacttcgt ttattcttaa aatatggtca aaacgatgag
    13921 cgtgttatca caggattaaa acgtatttca aaaccaggtt tacgtgttta tgcaaaagct
    13981 agcgaaatgc ctaaagtatt aaatggttta ggtattgcat tagtatcaac ttctgaaggt
    14041 gtaatcactg acaaagaagc aagaaaacgt aatgttggtg gagaaattat cgcatacgtt
    14101 tggtaataaa aaataaggag gtgccataac atgagtcgtg ttggtaagaa aattattgac
    14161 atccctagtg acgtaacagt aacttttgat ggaaatcatg taactgttaa aggtcctaaa
    14221 ggtgaattat caagaacttt aaatgaaaga atgacattca aacaagaaga aaacacaatt
    14281 gaagttgtaa gaccatctga ttctaaagaa gatagaacaa accatggtac aactcgtgct
    14341 ttattaaaca atatggtaca aggtgtttct caaggatacg taaaagtact tgaacttgtt
    14401 ggtgtaggtt accgtgctca aatgcaaggt aaagacttaa tccttaacgt tggttattct
    14461 cacccagtag aaattaaagc tgaagaaaac attactttct cagttgagaa aaacacagtc
    14521 gttaaagttg aaggtatttc aaaagaacaa gttggagcat tagcatctaa catccgttca
    14581 gtaagacctc cagagcctta caaaggtaaa ggtattcgtt accaaggtga atacgttcgc
    14641 cgtaaagaag gtaaaactgg taaataatag ataaactcta aagaaaggag tattgaaatg
    14701 atcagtaaaa ttgataaaaa taaagtgcgt ttaaaaagac atgctcgtgt tcgtactaac
    14761 ttatcaggta cagctgaaaa gccacgttta aacgtatatc gttcaaacaa gcatatctac
    14821 gctcaaatta ttgatgataa taaaggcgta acattagctc aagcttcttc aaaagacagc
    14881 gacattgcta ctacagcaac taaagttgaa ttagcaacta aagtcggtga agcaattgct
    14941 aaaaaagctg ctgacaaagg cattaaagaa atcgtatttg accgtggagg atatttatat
    15001 cacggacgtg ttaaagcatt agctgaagca gcaagagaaa gcggattaga attttaattt
    15061 aaaggaggga caaatacatg gctcgtagag aagaagagac gaaagaattt gaagaacgcg
    15121 ttgttacaat caaccgtgta gcaaaagttg taaaaggtgg tcgtcgtttc cgtttcactg
    15181 cattagttgt agttggagac aaaaatggtc gtgtaggttt cggtactggt aaagctcaag
    15241 aggtaccaga agcaatcaaa aaagctgttg aagcagctaa aaaagattta gtagttgttc
    15301 cacgtgttga aggtacaact ccacacacaa ttactggccg ttacggttca ggaagcgtat
    15361 ttatgaaacc ggctgcacct ggtacaggag ttatcgctgg tggtcctgtt cgtgccgtac
    15421 ttgaattagc aggtatcact gatatcttaa gtaaatcatt aggatcaaac acaccaatca
    15481 acatggttcg tgctacaatc gatggtttac aaaaccttaa aaatgctgaa gatgttgcga
    15541 aattacgtgg caaaacagta gaagaattat acaattaagg agggaaaact agttatggct
    15601 aaattacaaa ttaccctcac tcgtagtgtt attggtcgtc ctgaaacaca acgtaaaact
    15661 gttgaagctt taggtcttaa aaagactaac agttcagtag ttgttgaaga taaccctgct
    15721 attcgtgggc aaatcaacaa agttaagcac ttagtaacag tagaagaaaa ataatcaagg
    15781 aggtgccgaa atgaaattac atgagttaaa accggcagaa ggttcacgta aagaacgcaa
    15841 tcgtgttgga cgtggtgttg cgacaggtaa tggtaaaaca agtggtcgcg gacacaaagg
    15901 tcaaaaagct cgttcaggcg gtggtgtaag accaggattt gaaggtggtc aattaccatt
    15961 attccgtcgt ttaccaaaac gtggttttac taacataaat cgtaaagaat atgctattgt
    16021 taacttagac caacttaata aatttgaaga tggtactgaa gtaactccag ctttattagt
    16081 agaatctggt gttgttaaga atgaaaaatc tggtatcaaa atactaggta atggttcact
    16141 tgataagaaa ttgacagtga aagctcataa attctcagct tcagcagcag aagctattga
    16201 tgctaaaggt ggagcacacg aggtgatcta atgattcaaa cccttgtgaa cttctttaga
    16261 acaaaagagg ttcgtaacaa gattttcttc acactagcaa tgttagtaat ttttaaaata
    16321 gggacttata taccagctcc aggagtaaat cctgcagctt ttgataatcc ccaaggttct
    16381 caaggtgcca ctgagttatt aaatactttt ggtggcggag ccttgaaacg attttctatt
    16441 tttgcaatgg gtattgtacc ctacatcact gcatcaatcg taatgcaatt attacaaatg
    16501 gatattgtcc ctaaattctc agaatgggca aaacaaggtg aagtaggtag aagaaagtta
    16561 aataacgtta ctcgttattt agcaatttct ttagcattta tccaatctat aggtatggca
    16621 ttccaattta ataattatct caaaggtgcg ctgattatca atcagtcaat tatgagttat
    16681 ttattaatag cactagtttt gacagcagga actgctttct taatatggct tggtgatcaa
    16741 atcactcagt tcggtgttgg taatggtatt tctattatca tatttgcggg tattttatca
    16801 acgttgccag ccagtctaat tcaatttgga caaactgcgt tcgttgggca agaagataca
    16861 tcattagcat ggttgaaagt attaggactt ttagtgtcac tgatactatt aactgtaggt
    16921 gctatttatg tacttgaagc cgttcgtaaa attccgattc aatatgctaa gaaacaaact
    16981 gctcaaagat taggttcaca ggcaacttat ctacctttaa aagtgaactc agccggtgtt
    17041 attcctgtaa tctttgcaat ggcgttcttc ttactgccta gaacattaac gttgttctat
    17101 ccagataaag aatgggcaca aaacattgcg aatgctgcaa atccttcaag taatgttggt
    17161 atggttgtat atattgtact aattatacta ttcacatact tctatgcatt tgtacaagtt
    17221 aatccggaaa aaatggctga taaccttaag aaacaaggta gctatgttcc aggcattaga
    17281 cctggtgaac aaaccaaaaa atatattact aaagttttat atcgtttaac ttttgtaggt
    17341 tctatcttct tagccgttat ttcaattcta ccaatattag caactaagtt tatgggatta
    17401 ccacaatcaa ttcagattgg tggtacaagt ttacttatcg ttattggtgt agcgattgaa
    17461 acaatgaaat ctttagaagc tcaagtatct caaaaagaat ataaaggctt tggtggtaga
    17521 taattttagg agggcactta tgaatatcat tttgatgggt ttacctggcg caggtaaagg
    17581 aactcaagca agtgaaattg tcaagaaatt cccaataccc cacatttcaa ctggtgacat
    17641 gttcagaaaa gctataaaag aagaaactga attaggtaaa gaagctaagt cttatatgga
    17701 ccgtggcgaa ttagttcctg atgaagtgac tgtaggtatc gttaaggaaa gaatttctga
    17761 agacgatgca aaaaaaggct ttttattaga tggcttccca agaacaatcg agcaagctga
    17821 ggcattaaat aatattatgt ctgagcttga cagaaacatt gatgctgtca tcaatatcga
    17881 agttccggaa gaagaattaa tgaaccgtct tacaggtcgt cgaatctgtg agtcatgtgg
    17941 tacaacgtat catcttgtat ttaatcctcc gaaggtcgaa ggtatttgtg atatcgatgg
    18001 tggtaaattg tatcaacgag aagatgataa tcctgaaacg gtagctaatc gtttgagtgt
    18061 taatattaaa caatctaagc ctattttaga tttctatgtt caaaaaggtg tattgaaaaa
    18121 tattgatggt tcaaaagata ttagcgacgt taccaaagat gtcattgata ttttagatca
    18181 tttgtaatag atcaactttt agcggtctat tttaagtgag gcatgattta atcatattag
    18241 atagatgaga gtggcaatta acgataatta attcccgtaa tttttgttta acttataaaa
    18301 ttaatcactg atagattcgt aacaataaag ttgtctattg acgattacct tactattgca
    18361 aaaaggggga agttaatcaa tggctaaaca agatgtaatt gaattagaag gtactgtatt
    18421 agatacttta ccgaacgcaa tgtttaaagt agaattagaa aatggtcatg agattttagc
    18481 tcacgtaagt ggtaaaatca gaatgaatta cattcgtatt ctacctggcg acaaagtaac
    18541 tgttgagatg tctccgtacg atttaacacg cggaagaatt acttatcgtt ataaataatc
    18601 gtcactccat aatataggga ggtataaaaa tgaaagtaag accatcagta aaacctattt
    18661 gcgaaaaatg taaagtcatt aaacgtaaag gtaaagtaat ggtaatttgt gaaaatccaa
    18721 aacacaaaca aagacaaggt taataaaaga gaggtgtaaa ttaatatggc acgtattgca
    18781 ggagtagata ttccacgtga aaaacgcgta gttatctcat taacttatat atacggtatc
    18841 ggtacgtcaa ctgctcaaaa aattcttgaa gaagctaacg tatcagctga tactcgtgtg
    18901 aaagatttaa ctgatgacga attaggtcgc atccgtgaag ttgtagacgg ttataaagtc
    18961 gaaggtgact tacgtcgtga aactaactta aatatcaaac gtttaatgga aatttcatca
    19021 taccgtggta tccgtcaccg tcgtggttta ccagttcgtg gtcaaaaaac gaaaaacaac
    19081 gcgcgtactc gtaaaggacc agttaaaacg gtagctaaca agaaaaaata ataggtaaag
    19141 gaggcaaatt ttaaatggca cgtaaacaag tatctcgtaa acgtagagtg aaaaagaata
    19201 ttgaaaatgg tgtagcacac atccgttcaa cattcaacaa cactattgta actatcactg
    19261 atgagttcgg taatgcttta tcatggtcat cagctggtgc attaggattc aaaggatcta
    19321 aaaaatcaac accatttgca gcacaaatgg cttctgaaac tgcatctaaa tcagctatgg
    19381 agcatggttt aaaaacagtt gaagtaacag ttaaaggacc tggtccaggt cgtgaatcag
    19441 ctattcgtgc attacaatct gcaggtttag aagtaactgc gatcagagac gttactccag
    19501 tacctcataa cggttgtcgt ccaccaaaac gtcgtcgtgt ataatttatg atggtattgt
    19561 tacaggtcac tgagcaaaca ttttaaatta agtcgacgta tataaggagg atatttaaat
    19621 gatagaaatc gaaaaaccta gaattgagac aattgaaatt agtgaagatg ctaaattcgg
    19681 taagttcgtt gttgaaccac tagaacgtgg ctacggtact acactaggaa actccttacg
    19741 tcgtatccta ctatcttcat taccaggtgc agccgttaag tatattgaaa ttgagggagt
    19801 tttacatgaa ttctcagcag tagacaatgt agttgaagat gtttctacaa ttattatgaa
    19861 cattaaacaa ttagcattga aaatttactc tgaagaagat aaaactttag aaattgatgt
    19921 acgtgatgaa ggcgaagtaa cagcaagcga cattacacat gatagtgatg ttgaaatttt
    19981 aaacccagag cttaaaattg caacagtatc taaaggtggt cacttaaaaa ttcgtctagt
    20041 tgctaacaag ggtagaggtt acgcattagc agaacaaaat aatactagtg atttaccaat
    20101 tggtgtaatc cctgttgatt cattgtattc acctgttgaa cgtgtgaact atactgttga
    20161 aaatacacgt gtaggtcaaa gcagtgattt tgataaatta acattggatg tttggactaa
    20221 tggttcaatc acaccacaag aatcagtttc attagcagca aaaataatga ctgaacactt
    20281 gaatatcttc gttggtctta ctgatgaagc gcaaaacgct gaaatcatga ttgaaaaaga
    20341 agaagatcaa aaagaaaaag tattagaaat gtctattgaa gaattagact tatctgtacg
    20401 ttcatataac tgcttaaaac gcgcaggaat caattctgtt caagagttag ctgacaaatc
    20461 tgaagctgac atgatgaaag tgcgtaattt aggtcgtaaa tctttagaag aagttaaata
    20521 caaattagaa gatttaggat taggattaag aaaagaagat tgataaagga ggttaactca
    20581 tgggttacag aaaattaggt cgtacttctg atcaacgtaa agctatgtta cgtgacttag
    20641 ctacatcact tattattagt gaacgtattg aaactacaga agctcgtgca aaagaagttc
    20701 gcagtgttgt tgagaaatta atcactttag gtaaaaaagg agatttagct tctcgtcgta
    20761 atgcagctaa aactttacgt aatgttgaaa tcttaaacga agatgaaact acacaaactg
    20821 cacttcaaaa attatttggt gaaatcgcag agcgttacac agaacgtcaa ggtggttaca
    20881 ctcgtatcct taaacaaggc cctcgtcgcg gtgacggtgc tgaatcagta attatcgaat
    20941 tagtataaga atttatataa atacactaac tacttttata taaagcaaat gagtgcaacg
    21001 ataatgtttg ccacatacaa atattgtcta gctcagagta ccccatcaac taaataaaga
    21061 tttaaagcgt gaactcaaca aacttatgat ggggtgcgcg cttttttatt gaataagata
    21121 tcggacttat gaacagaaat gtttttaagt ccgatttctt tatatctact tttttgagtg
    21181 cgaatctaaa aatatgatca agctagtttg atcaatttca ctagaactga atgaagtgaa
    21241 agtattatcg ttgaaccaaa cgcgaccacc cctatattag taaatcattt attttattaa
    21301 cgtttataca taattgttag agaggaataa atgtccttat cattagttgt taaacaacac
    21361 tttaaagact agaaatgagt tagaaaatac tagtaatata tagcaaaccc agtcaatgtc
    21421 atatacagcg atttataaat attgtaaaat atgatagata cacgaatcgt aaaggagaga
    21481 gacaagtgga ggataagaat tcagttattg tatttaaaaa tgtttcattt caatatcaaa
    21541 gtgatgcatc cttcacattg aaagatgttt cttttaatat acctaaaggt cagtggacat
    21601 ctattgttgg tcataacggt tctggaaaat ctacaattgc caagttaatg attggcatag
    21661 agaaagttaa atctggagaa attttttata ataatcaagc tataactgat gataattttg
    21721 aaaagttaag aaaagacata ggaattgtat ttcagaatcc ggataatcaa tttgttggtt
    21781 caattgtaaa atacgatgtg gcatttggac tcgaaaatca tgcggttcca catgacgaaa
    21841 tgcatagaag agtcagcgaa gcacttaaac aagttgatat gttagaacgt gcagattatg
    21901 aacctaatgc attatcgggg ggacagaagc agcgtgtggc tatagcaagt gtattagcac
    21961 ttaacccctc tgtcattata ttagatgagg cgacttctat gttagatcct gatgcacgtc
    22021 aaaatttatt ggatttagtg agaaaagtta aatcagaaca taatattaca atcatttcta
    22081 ttacgcatga tttatctgag gcgatggaag cagatcatgt tatcgttatg aataaaggga
    22141 ctgtctataa agaaggcaca gcgactgaaa ttttcgacca tgcagaagag ttaacaagaa
    22201 taggtctaga tttgccattc ccaatcaaaa taaatcaaat gctgggacac caaacatcat
    22261 tcttaactta tgaagggctg gtggatcaac tatgactata cggtttgaca atgtaagtta
    22321 tacctatcaa aaagggacac catatcagca tcaagctatt catgatgtta atacagaatt
    22381 tgaacaaggt aaatattacg ccatcgttgg acaaacgggt agtggtaaat caacgttgat
    22441 acaaaatatt aatgcgctgt taaagccgac tactgggaca gttacagttg atgacattac
    22501 tattacacat aagaccaaag ataaatatat tagacctgta agaaaaagaa ttggaatggt
    22561 atttcaattt cccgaatctc aattatttga ggacacagta gagcgtgaaa tgatatttgg
    22621 acctaaaaac tttaaaatga atttagatga agccaaaaac tatgcccatc gtctgttgat
    22681 ggatttaggc ttttcaagag atgtaatgtc acaatcacca tttcaaatgt caggtggaca
    22741 aatgcgtaaa atagcgattg tatcgatatt ggcaatgaat cctgatatta tcgtggttga
    22801 tgaacctaca gcaggacttg atccacaaag taaacgacaa gtaatgagat tactaaagtc
    22861 actacaaaca gatgaaaata aggcaattat cctaatttca catgatatga atgaagtcgc
    22921 gcgttatgca gatgaagtca tcgttatgaa agaaggtagt atagtgtcgc aaacatcacc
    22981 aaaagagctc ttcaaagata aaaaaaaatt ggcggattgg catattggtt tgccagaaat
    23041 tgttcagtta caatatgact ttgaacaaaa atatcaaaca aaattaaaag atattgcctt
    23101 aactgaagaa gcatttgtaa gcttgtataa ggagtggcaa catgaaaaat aaattgatta
    23161 tagggcgata tttaccaatt aattcttttg ttcatcatct tgatccaaga gcaaagttaa
    23221 tgtt
//
LOCUS       DAOBBH010000023        23125 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.023, whole genome shotgun sequence.
ACCESSION   DAOBBH010000023 DAOBBH010000000
VERSION     DAOBBH010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 23125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23125
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.023"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            390..854
                     /gene="accB"
                     /locus_tag="P6L01_000650"
     CDS             390..854
                     /gene="accB"
                     /locus_tag="P6L01_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDP3029738.1"
                     /translation="MNFKEIKELIEILDKSTLTEINIEDTKGKVTLKKEKETEIITPQ
                     ISQMPVEAAAMPMPQAQSTDSNKTEAPKPTSDNHKTINAPMVGTFYKSPSPDEEAYVQ
                     VGDTVSNETTVCILEAMKLFNEIQAEISGEIVEILVEDGQMVEYGQPLFKVK"
     gene            854..2209
                     /gene="accC"
                     /locus_tag="P6L01_000651"
     CDS             854..2209
                     /gene="accC"
                     /locus_tag="P6L01_000651"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDP3029739.1"
                     /translation="MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIA
                     DEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFI
                     GPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATA
                     GGGGKGIRVARDEKELETGFRMTEQEAQTAFGNGGLYMEKFIENFRHIEIQIVGDSYG
                     NVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFI
                     YDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYKQEDIKLTGHA
                     IEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESACYTNYTIPPYYDSMVAKLIIH
                     EPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQNSIMNDE
                     G"
     gene            2224..2586
                     /locus_tag="P6L01_000652"
     CDS             2224..2586
                     /locus_tag="P6L01_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDP3029740.1"
                     /translation="MVKVTDYSNSKLGKVEIAPEVLSVIASIATSEVEGITGHFAELK
                     ETNLEKVSRKNLSRDLKIESKEDGIYIDVYCALKHGVNISKTANKIQTSIFNSISNMT
                     AIEPKQINIHITQIVIEK"
     gene            2646..3035
                     /gene="nusB"
                     /locus_tag="P6L01_000653"
     CDS             2646..3035
                     /gene="nusB"
                     /locus_tag="P6L01_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDP3029741.1"
                     /translation="MSRKESRVQAFQTLFQLEMKDSDLTINEAISFIKDDNPDLDFEF
                     IHWLVSGVKDHEPVLDETISPYLKDWTIARLLKTDRIILRMATYEILHSDTPAKVVMN
                     EAVELTKQFSDDDHYKFINGVLSNIKK"
     gene            3052..4389
                     /locus_tag="P6L01_000654"
     CDS             3052..4389
                     /locus_tag="P6L01_000654"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDP3029742.1"
                     /translation="MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHL
                     YFNVKDKESVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLD
                     GIGNLYQKLEALKKKLTEEGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSR
                     FPLAEQIQISTLVQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVV
                     RAIYNCKTPIISAVGHETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFT
                     LTRFIKKHLEQQRKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQR
                     HRLMLLQQRYNLKALLSSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNL
                     SPTNTMLRGYAIVNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND"
     gene            4382..4612
                     /locus_tag="P6L01_000655"
     CDS             4382..4612
                     /locus_tag="P6L01_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDP3029743.1"
                     /translation="MTKETQSFEEMMQELEQIVQKLDNETVSLEESLDLYQRGMKLSA
                     ACDTTLKNAEKKVNDLIKEEAEDVKNDESTDE"
     gene            4590..5471
                     /locus_tag="P6L01_000656"
     CDS             4590..5471
                     /locus_tag="P6L01_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDP3029744.1"
                     /translation="MTNLPMNKLIDEVNNELSVAINKSVMDTQLEESMLYSLNAGGKR
                     IRPVLLLLTLDSLNTEYELGVKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKV
                     YGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQS
                     EGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDATKEHLESYSYHLGMMFQIKD
                     DLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELTQIDEQF
                     NTKHLLEIVDLFYSRDH"
     gene            5903..6355
                     /gene="argR"
                     /locus_tag="P6L01_000657"
     CDS             5903..6355
                     /gene="argR"
                     /locus_tag="P6L01_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDP3029745.1"
                     /translation="MPKKSVRHIKIREIISNEQIETQDELVKRLNDYDLNVTQATVSR
                     DIKELQLIKVPIPSGQYVYSLPNDRKFHPLEKLGRYLMDSFVNIDGTDNLLVLKTLPG
                     NAQSIGAILDQINWEEVLGTICGDDTCLIICRSKEASDEIKSRIFNLL"
     gene            6371..8050
                     /gene="recN"
                     /locus_tag="P6L01_000658"
     CDS             6371..8050
                     /gene="recN"
                     /locus_tag="P6L01_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDP3029746.1"
                     /translation="MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQ
                     LIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHILKNMDIDVDEDFLLVKREIFSSG
                     KSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLD
                     KYHQTFQNYKAKKQELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIK
                     RIQNSEKLSLALNNAHMTLTDENAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFY
                     YILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKDISELIAYQEKLNNE
                     INKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIVARELRDHIVSEIQNLQMKD
                     ANLEISFKKLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVK
                     SRGQTAILFDEVDSGVSGQAAQKMAEKMRDIAEYIQVICISHLPQVASMSDHHLLISK
                     SSKDDRTTTQVQELIGDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR"
     gene            8201..9622
                     /gene="lpdA"
                     /locus_tag="P6L01_000659"
     CDS             8201..9622
                     /gene="lpdA"
                     /locus_tag="P6L01_000659"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDP3029747.1"
                     /translation="MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCL
                     HKGCIPTKSLLKSAEVFQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHL
                     MQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPF
                     LSFDHDKILSSDDILSLKTLPSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILP
                     TESKQASQLLKKSLSARGVKFYEGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIG
                     RKPNTSDIGLNNTKIKLSTSGHILTNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVV
                     AVDHMFEGNPIPVNYNMMPKCIYSQPEIASIGLNIEQAKAEGMKVKSFKVPFKAIGKA
                     VIDSHDANEGYSEMVIDQSTEEIVGINMIGPHVTELINEASLLQFMNGSALELGLTTH
                     AHPSISEVLMELGLKAESRAIHV"
     gene            9638..10630
                     /locus_tag="P6L01_000660"
     CDS             9638..10630
                     /locus_tag="P6L01_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HDP3029748.1"
                     /translation="MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVV
                     SGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGK
                     QMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFH
                     EGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGIHGEQVDGNDPLA
                     MYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEEREALKKADCNEKFK
                     KELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN"
     gene            10630..11613
                     /locus_tag="P6L01_000661"
     CDS             10630..11613
                     /locus_tag="P6L01_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDP3029749.1"
                     /translation="MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGKKGGVFGTTQGL
                     QQQYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMR
                     YRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLS
                     SIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCL
                     QAADILAADGINVEVVDLRTVYPLDKETIIDRAKNTGKVLLVTEDNLEGSIMSEVSAI
                     IAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF"
     gene            11626..12900
                     /locus_tag="P6L01_000662"
     CDS             11626..12900
                     /locus_tag="P6L01_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDP3029750.1"
                     /translation="MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTA
                     EVPSTISGTITEILVEAGQTVAIDTIICKIETADEKTNETTEEIQAKVDEHTQKSTKK
                     ASATVEQTFTAKQNQPRNNGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDIMSVI
                     ENGGTTAQSDKQVQTKSTSVDTSSNQSSEDNSENSTIPVNGVRKAIAQNMVNSVTEIP
                     HAWMMIEVDATNLVNTRNHYKNSFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQG
                     NEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTLATKARNKQLTTEDM
                     QGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIVKKPVVINDMIAIRSMVNLCISI
                     DHRILDGLQTGKFMNHIKQRIEQYTLENTNIY"
     gene            13251..13688
                     /locus_tag="P6L01_000663"
     CDS             13251..13688
                     /locus_tag="P6L01_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HDP3029751.1"
                     /translation="MDMNFDLYMNGVVEQARNEIESAGYEQLTTAEDVDKVLKQDGTT
                     LVMINSVCGCAGGIARPAASHALHYDVLPDRLVTVFAGQDKEATQRAREYFEGYAPSS
                     PSFALVKDGKITEMIERHQIEGHDVMNVINQLQTLFNKYCEER"
     gene            13702..14682
                     /locus_tag="P6L01_000664"
     CDS             13702..14682
                     /locus_tag="P6L01_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDP3029752.1"
                     /translation="MLKLNPYKIGFRTIKTAVGMTLGVIISKLLGLDNYASSAILVVL
                     CIKHTKVHSLQAIISRLVSCFLVLFLGSAIFSLLGQSPIVLGIIVLLFIPLTVVLKVQ
                     EGVITSCVILLHVFNAKSIDAHLIVNETLLLLIGLSIAFTMNLMMPSLDKQLDEYKCK
                     IEQQIADIFSKYSYICEKYEDTIAIEFEVLLLNIKKAKSIAFRDVKNHFVRNENSYYH
                     YFDMREEQVELLMRMKPLIESICHKDPLLNDVSSLLKEIGLNVNSNDYTAMRLHNLYE
                     TRLKLDELPLPTTHKALNSRASIIQILNELEEYLQIKSQFGSLKMHSEVA"
     gene            complement(14809..14988)
                     /gene="prli42"
                     /locus_tag="P6L01_000665"
     CDS             complement(14809..14988)
                     /gene="prli42"
                     /locus_tag="P6L01_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stressosome-associated protein Prli42"
                     /protein_id="HDP3029753.1"
                     /translation="MLVISSQVKSFISYVVIINTKVIHLEAHKMQNKVLRIIIIVMLV
                     SVVLALLLTSIIPIL"
     gene            15251..16384
                     /locus_tag="P6L01_000666"
     CDS             15251..16384
                     /locus_tag="P6L01_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDP3029754.1"
                     /translation="MINEQRLLNTFLELVQIDSETGNESTIQPILKEKFIALGLDVKE
                     DEAAKHPKLGANNLVCTMNSTIEEGEVPKLYLTSHMDTVVPAINVKPIVKDDGYIYSD
                     GTTILGADDKAGLAAMLEVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDA
                     DFGYAIDASADVGTTVVGAPTQMLISAKIIGKTAHASTPKEGVSAINIAAKAISRMKL
                     GQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASE
                     LGGKAEVTVEQSYPGFKINDNEAVVKIAQESARNLGLSANTIISGGGSDGSIINTFGI
                     PSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARQSK"
     gene            16452..17858
                     /gene="gndA"
                     /locus_tag="P6L01_000667"
     CDS             16452..17858
                     /gene="gndA"
                     /locus_tag="P6L01_000667"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDP3029755.1"
                     /translation="MTQQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEE
                     SKGKNIHPTYSLEEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNT
                     NYQDTIRRNKALAQSAINFIGMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAA
                     KAKDGASCVTYIGPNGAGHYVKMVHNGIEYADMQLIAESYAMMKELLGMSHEDIAQTF
                     KDWNAGELESYLIEITGDIFMKLDENKEALVEKILDTAGQKGTGKWTSINALELGIPL
                     TIITESVFARFISSIKEERVNASKELNGPKASFDGDKKDFLEKIRKALYMSKICSYAQ
                     GFAQMRKASEDNEWNLKLGDLAMIWREGCIIRAQFLQKIKDAYDNNPGLQNLLLDPYF
                     KNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAH
                     TYERKDKEGVFHTQWIEE"
     gene            18174..19391
                     /locus_tag="P6L01_000668"
     CDS             18174..19391
                     /locus_tag="P6L01_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDP3029756.1"
                     /translation="MSYTSVLFNKNFTFYLIGIAFSNMGSAFTTFVFPLMILKLTGSA
                     FQVGIVSALSFIPYAILGLPAGALIDRLNKKTIMKCADIIRFVSYLSIPVLSFFNMLS
                     IFQIYVVAIISGIGLVFHSISEVSIIPSIVKEEDLASANSYIYATQNVSEFLGPIIGG
                     LLYTYMGYSILIFIDSMTFLLSFFSLILIKIETKSIFNQEKLSGKNFLSDVKVGFDYL
                     LSNSTIRVMAVVVSLSNLIISPYYIYIVMFVKEDMNQSSQALGLVLGISSLGALIGSL
                     SASFLLKLFNFGKLIVIILFLDTIFRLMLPFSTYIFILIPLLGLTYMTQSILNIAIIT
                     LRQKKCSENMLGRVNSVFKTMVFAFRPIGLFLGGILLENKGGFYALTISAVLFIPLVL
                     YILKNRFYQVEEY"
     gene            19858..20229
                     /locus_tag="P6L01_000669"
     CDS             19858..20229
                     /locus_tag="P6L01_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDP3029757.1"
                     /translation="MEKLNQVMLYVDDVEKAKAFWTETLEFVVVSETPLAEDYVAVEV
                     SPTKDAETSLTIMAKEFIEKHSPEVNLGTPSLMFKEKNFDALYSKLNDLGLTGHDIVE
                     MDGQRVFNFQDGQGNYFAVSD"
     gene            20168..20422
                     /locus_tag="P6L01_000670"
     CDS             20168..20422
                     /locus_tag="P6L01_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029758.1"
                     /translation="MVNVYLISKMDKVIILQLVTKQSILIFIQTFILIFGLNFSMKVL
                     FLSFYAIVCTNLLTFLERVTKLLLYRGWKRFPMQKLTGGD"
     gene            20423..21442
                     /locus_tag="P6L01_000671"
     CDS             20423..21442
                     /locus_tag="P6L01_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDP3029759.1"
                     /translation="MVTIKDVALKAGVSPSTVSRVIKGNKRISEATISKVKKVMEELN
                     YFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVS
                     NNMNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDIDHQF
                     THIDNDNILASENLTRHVIEQGVDELIFITEKGNFEVSKDRIQGFETVASQFNLDYQI
                     IETSNEREVILNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDVMT
                     ATFNDSYLTEIASPPQTCIDIKPRMLGQQAGSAILNILKNKAQDVIELVIIDTELKIR
                     KSTQR"
     gene            21458..23107
                     /locus_tag="P6L01_000672"
     CDS             21458..23107
                     /locus_tag="P6L01_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDP3029760.1"
                     /translation="MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDLG
                     IDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDCLLKGIHDRGMKLILDLVVN
                     HTSDEHPWFIESKSSKDNPKRDWYIWQDPKPDGSEPNNWESIFNGSTWEYDANTEQYY
                     FHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPE
                     GKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGK
                     FNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTW
                     GDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIV
                     KAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNLNYKTINVAQQL
                     EDEHSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTN
                     HEAELTVPFDLSHGEVKLFNYDAKVNLKQLRPYEACVIELN"
BASE COUNT         8476 a         3115 c         4362 g         7172 t
ORIGIN      
        1 taaaatgatt ataggttata tgctgatatg aggcttttta tttttaaaat aatttttaaa
       61 atataaaacg aatcgtctaa ttggtagtga aaaaacatag acatgagtaa aggtatatat
      121 ctacatatca tattgttgag gataaaacat aatagctaat aaaaataaca ttgatatgtt
      181 taactgaatg cataaaattt ttaatcaaga gttaagatga gtcaatgtta agtaataaat
      241 acacaagtgt ttttaattta aaatgtggtt ttattataac tttgggatag acttataatg
      301 tcgccagcaa tatgaacatg cttgaattga agagttgtct caagtaaaat agacgggtag
      361 atgaaaacaa actgaaggag tcagtaataa tgaactttaa agaaatcaaa gaattaattg
      421 aaattctgga taaatcaact ttaacggaaa tcaatattga agatactaaa ggcaaagtga
      481 cgcttaagaa agaaaaagaa actgagatta tcacgccaca aatctcacaa atgccagttg
      541 aagctgcggc aatgcctatg cctcaagcac aatcaactga tagcaataaa actgaagctc
      601 caaagccaac ttcagataat cacaaaacaa ttaatgcacc tatggtaggt acattttaca
      661 aatcgccatc tccagacgaa gaagcatatg tgcaagttgg ggacactgtt tcaaatgaaa
      721 caacagtgtg tattttagag gcaatgaaac tatttaatga aattcaagca gaaatttcag
      781 gtgaaattgt tgaaatctta gtagaagacg gacaaatggt agagtatggc caaccgttat
      841 ttaaggtgaa ataatgaaaa aggttttaat tgcaaaccgc ggtgaaatcg cagttaggat
      901 tattcgcgct tgtcgtgatt taggcatcca aactgttgca atctattctg aaggggataa
      961 agatgcgcta catactcaaa ttgctgatga agcatattgc gtaggtccca ctttgtctaa
     1021 agattcatat ttaaatattc cgaacatctt atctattgca acttctacag gttgtgatgg
     1081 cgttcatccg ggttatggct ttttagctga aaatgctgac tttgcagaat tatgcgaagc
     1141 atgccaattg aagttcattg gaccaagtta tcaatctatc caaaaaatgg gtatcaaaga
     1201 tgttgctaag gcagaaatga tcaaagccaa tgttccagtt gttcctggta gtgacggttt
     1261 aatgaaagac gtctcagaag ctaagaaaat cgctaagaaa attggctatc cggtcatcat
     1321 taaagctact gctggtggtg gcggaaaagg tatccgtgtt gctcgtgatg aaaaagaact
     1381 tgaaactggc ttccgaatga cagaacaaga agctcaaact gcatttggta atggtggact
     1441 ttatatggag aaattcatcg aaaacttccg ccatattgaa atccaaattg ttggggacag
     1501 ctatggtaat gtaattcatt taggagaacg tgattgtaca attcaaagac gtatgcagaa
     1561 attagtggaa gaagcacctt ccccaatttt agatgatgaa acacgtcgtg aaatgggaaa
     1621 tgccgcagtt cgtgcagcga aagctgtaaa ttatgaaaat gcgggaacaa ttgagtttat
     1681 atatgattta aatgataata aattttattt tatggaaatg aatacacgta ttcaagtaga
     1741 acatcctgta actgaaatgg taacaggaat tgatttagtt aaattacaat tacaagttgc
     1801 tatgggtgac gtgttaccgt ataaacaaga agatattaaa ttaacaggac acgcaattga
     1861 atttagaatt aatgctgaaa atccttacaa gaactttatg ccatcaccag gtaaaattga
     1921 gcaatatctt gcaccaggtg gatatggtgt tcgaatagag tcagcatgtt atactaatta
     1981 tacgataccg ccatattatg attcgatggt agcgaaatta atcatacatg aaccgacacg
     2041 agatgaagcg attatggctg gcattcgtgc attaagtgaa tttgtggttc ttggtattga
     2101 tacaactatt ccattccata ttaaattatt gaataacgat atatttagaa gcggtaaatt
     2161 taatacaaac tttttagagc aaaatagcat tatgaatgat gaaggttaat aggaggtcaa
     2221 tccatggtca aagtaactga ttattcaaat tcaaaattag gtaaagtaga aatagcgcca
     2281 gaagtgctat ctgttattgc aagtatagct acttcggaag tcgaaggcat cactggccat
     2341 tttgctgaat taaaagaaac aaatttagaa aaagttagtc gtaaaaattt aagccgtgat
     2401 ttaaaaatcg agagtaaaga agatggcata tatatagatg tatattgtgc attaaaacat
     2461 ggtgttaata tttcaaaaac tgcaaacaaa attcaaacgt caatttttaa ttcaatttct
     2521 aatatgacag cgatagaacc taagcaaatt aatattcaca ttacacaaat cgttattgaa
     2581 aagtaatgtc atacctaatt cagtaattaa ataaagaaaa atacaaacgt ttgaaggagt
     2641 taaaaatgag tcgtaaagaa tcccgagtgc aagcttttca aactttattt caattagaaa
     2701 tgaaggacag tgatttaacg ataaatgaag cgataagttt tattaaagac gataatccag
     2761 atttagactt tgaatttatt cattggctag tttctggcgt taaagatcac gaacctgtat
     2821 tagacgagac aattagtcct tatttaaaag attggactat tgcacgttta ttaaaaacgg
     2881 atcgtattat tttaagaatg gcaacatatg aaatattaca cagtgataca cctgctaaag
     2941 tcgtaatgaa tgaagcagtt gaattaacaa aacaattcag tgatgatgat cattataaat
     3001 ttataaatgg tgtattgagt aatataaaaa aataaaattg agtgatgtta tatgtcagat
     3061 tatttaagtg tttcagcttt aacgaaatat attaaatata aatttgatca agatccacat
     3121 ctccaatctg tattgattaa aggtgagctt tcaaatttca aaaagcattc aagtggtcat
     3181 ttatacttta atgtgaaaga taaagagagc gttataagtg ccatgatgtt caaaggtagt
     3241 gcttctaaat taaacttcga acctaaagaa ggagatgaag tcttattaga agcacgtgtt
     3301 tctgtttttg aacgtcgtgg aaactatcaa atttatgtaa ataaaatgca attagatggt
     3361 atagggaact tatatcaaaa attagaagca ttaaagaaaa aattaactga agaaggttgt
     3421 tttgataaag cgaataagaa atcaatacct aagtttccta aaaaaattgc agttttaaca
     3481 gcgagtacag gtgccgcaat tcgagatatc cattcaacga taaatagtcg ttttccatta
     3541 gctgaacaaa tacaaattag tactttggtt caaggtgaaa aagcaaaaga cgacattatt
     3601 gaaaaaattg aatatgcaga tagtttaggt gtagatacca ttattgtagg tcgaggtggt
     3661 ggatccatag aagatttatg gaatttcaac gaagaagctg tcgtccgtgc aatatataat
     3721 tgtaagacac cgattatatc agcagtaggt catgaaacag actttacatt aagtgatttt
     3781 gctgcagaca tcagagctgc gactccaact caagctgctg ttattgcaac accagaccaa
     3841 tatgaattgc tgcaacaaat acagcaatat cagtttacat tgactcgttt catcaagaaa
     3901 catttagaac aacaacgtaa acatgttgaa catttgtcat cgtattacaa gtttaaacaa
     3961 ccaactttat tgtatgatca acagatacaa cgtcgagatg atttagaaaa gagactgaaa
     4021 caacaaattc aggcaacttt tgaacaacag agacatcgat taatgctgtt gcaacaacgt
     4081 tataatttaa aagcattact aagtagtgtt aatcaagaac aacaaaataa tcttcaattg
     4141 acaaaccaac ttgttaaatt attgaattct aagatattaa gttataaaaa tgatttgaaa
     4201 aataaagtag aaaacttaaa caatttaagt ccaactaata caatgttgcg tggatatgca
     4261 attgttaata aaaaagacga agtcattacg agtactaaag atttaacaga aaatgatcaa
     4321 ttgacgttaa caatgaaaga tggcttagta gatgcaaaag ttacgaaagt aaggtgtaat
     4381 aatgactaaa gaaacgcaaa gttttgaaga aatgatgcaa gaattagagc aaattgttca
     4441 aaaattagat aatgaaacag tatctttaga ggaatcatta gatttatatc aacgtggtat
     4501 gaaactatca gcagcttgtg acacaacttt aaaaaatgcc gaaaaaaagg tgaatgactt
     4561 aataaaagaa gaagctgagg atgtaaaaaa tgacgaatct accgatgaat aaattaatag
     4621 atgaagtcaa taatgaatta tcggttgcga taaataaatc agtaatggat actcagctag
     4681 aagaaagcat gttgtattca ttaaatgctg gaggtaaacg catccgacca gttctgttat
     4741 tactcacttt agattcacta aataccgagt atgagttagg tgtgaagagc gcaattgcac
     4801 tagaaatgat tcatacatat tcacttattc atgatgacct accagcgatg gataatgatg
     4861 attatcgacg agggaaatta acaaatcata aagtatatgg tgagtggact gcgatattag
     4921 caggtgatgc tttattaact aaagcatttg aacttatttc aagtgatgat agattaactg
     4981 atgaagtaaa aataaaagtt ctacaacggc tgtcaatagc aagtggtcat gttggaatgg
     5041 tcggcggtca aatgttagat atgcaaagcg aaggccaacc aattgatctt gaaactttgg
     5101 aaatgataca caaaacaaaa acaggagcat tattaacttt tgcggttatg agtgcagcag
     5161 atatcgctaa tgtcgatgat gcaactaaag aacatttaga aagttatagt tatcatttag
     5221 gtatgatgtt ccagattaaa gatgatttat tagactgcta tggtgatgaa gcaaagttag
     5281 gtaaaaaagt gggcagcgat cttgaaaata ataaaagtac gtacgtgagt ttattaggga
     5341 aagatggcgc agaagataaa ttgacttatc atagagacgc agcagtggat gaactaacgc
     5401 aaattgatga acaattcaat acaaaacact tattagaaat cgttgattta ttttatagca
     5461 gggatcacta aaagcaaata tatcgattag caacaattgt aaagtttgaa atgtctcaaa
     5521 aggcatattc aactgacgtt gtttgttaat cgatttttct catgttgaaa cttaaaatta
     5581 tgttgaagac tttcatttaa aaaatagaat tttcaatttc tatacgacaa tattcgtatc
     5641 tgtgacatca atgtgatttt aattaaatat gttcaaacaa ttgacgctta ttaaatctaa
     5701 taattagcaa gtttattaca catatattca tttttataca tattttaagc aattgttatg
     5761 cgtaatatga aaaatattta taaatagata accaagatag ataaaccatg tttacccttt
     5821 ttacgtaaag gtgtttagta gtcgtaaatc agtgcatgtg ttaaaataaa tgtataaata
     5881 ttcgtaatca gaggtgttta ctgtgcccaa aaaatcggtt aggcatataa aaattagaga
     5941 aattatttca aatgaacaga tagagacaca agatgaatta gttaaacgat taaacgatta
     6001 tgatttaaat gtcactcaag caactgtttc tcgtgatatt aaagaactac aacttattaa
     6061 agtacctata ccttcaggtc aatatgttta tagtttacca aatgatagaa aattccatcc
     6121 tttagaaaaa ttgggacgtt atttaatgga ttcctttgtt aatatagatg gtactgataa
     6181 tttacttgtt ctaaaaacat tacctggtaa tgcacaatct attggagcta tattagacca
     6241 aatcaattgg gaagaagtac taggcacaat ttgtggtgat gatacttgtt taattatttg
     6301 tcgaagcaaa gaggcaagtg atgaaatcaa gtcaagaatt ttcaatttgt tataaggatg
     6361 cgatgagttt atgttacaaa ccttatcaat caagcaattt gctattattg aagaattaga
     6421 aattcaattt tctgatggtt taactgtttt aagtggtgaa acgggttcag gtaagtcaat
     6481 tattatagat gcaattggac aattaattgg aatgagagct tcatctgatt ttgtaagaca
     6541 tggtgaaaaa aaggctgtca tcgaaggtat atttgatatt gacgagagta aagatgctat
     6601 acatatattg aaaaatatgg atattgatgt agatgaagat tttttattag ttaaacggga
     6661 aatttttagc tctggtaaaa gtctttgtaa aattaataat caaacagtta ctttacaaga
     6721 tttacgaaaa gttatgcaag aattattaga tattcatggc caacatgaaa cgcagtcttt
     6781 gttgaaacaa aaataccatt taacattatt ggataattac gcagaatcac gttatcaaga
     6841 tttgttggat aaatatcacc aaacttttca aaattataaa gccaaaaagc aagagttaga
     6901 agatttagaa tcagcagacc aagcattgct gcagcgttta gatttaatga aattccaatt
     6961 agaagaattg tctgaagcac atctaaaaga aggcgaaata gagcaacttg aaatagatat
     7021 taaacgcatt caaaactctg aaaaattaag cttagcgctt aataacgcac atatgacatt
     7081 aacggatgaa aatgctatca ctgatcgttt gtatgaacta agcaaccatt tgttaacaat
     7141 aaacgatata gttccaaata aatatgacaa attaaaagaa gatattgatc aattttatta
     7201 cattttagaa gatgcaaaac atgagttata tgatgaaatg gctaatacag aattcgacga
     7261 acaagtgtta aacgagtatg aatctcgtat gaatttgctg aataatttaa aacgtaaata
     7321 tggaaaagat atttcagaat taatcgcata tcaagaaaag cttaataatg aaatcaataa
     7381 aattgaaaac tatgaacaaa gtacatcgca gttacgagaa gaaataaatg cattgtataa
     7441 tcaagttata gaggttggac aagcgttatc aaagcagcgt cgtatagtcg ctagggagtt
     7501 aagagaccac attgtatcgg aaattcaaaa cttacaaatg aaagacgcaa atcttgagat
     7561 ttcatttaaa aaattagaag aaccgaatat tgatggaatc gaatttgtag aatttttaat
     7621 cagtccaaat aaaggggaac cattaaaaag tttaaataaa attgcatcag gtggagaact
     7681 ttctagaatt atgttagcgt taaaaagtat ttttgttaaa tcgagaggtc aaactgcaat
     7741 tttattcgat gaggttgact caggtgtatc tggacaagcc gcacaaaaaa tggcagagaa
     7801 aatgcgagat atagcagaat atatacaagt tatttgtatt tctcacttgc cacaagttgc
     7861 atcgatgagc gaccatcatt tattaatttc gaagtcatca aaagatgatc gtacaacaac
     7921 acaagttcaa gagttaattg gcgatgataa agtagatgaa attgcacgaa tgatttcagg
     7981 tgcaagtgtt actgatttaa ctcgagaaaa tgcaagagaa atgatacaac acaatcaaag
     8041 acgtagataa tttccaaagt gtgttgaata ctcatttatg gatttattta tgtgcattca
     8101 aactaaatag acaatcatgt aaaataggta attaatggac atgaaaacta tgtattaata
     8161 aattttcaat ggatatatat cattagttag gagtaagaac atgtcagaga aacaatatga
     8221 tttagtcgtt ctcggtggag gtactgcagg atatgttgct gcgatacgtg cgtctcaatt
     8281 aggtaagaaa gtggcaatag tagaacgaca actattagga ggtacttgtc tccataaagg
     8341 ttgtattcct actaaatcgc tgcttaaatc tgctgaagta tttcaaacag taaaacaggc
     8401 agcaatgttt ggcgttgatg tcaaagatgc taatgttaat tttgaaaata tgttggcacg
     8461 aaaagaagac attattaatc aaatgtatca aggtgtaaag catttaatgc aacacaatca
     8521 cattgacatt tataatggca cgggacgtat tctaggtaca tctatatttt cacctcaaag
     8581 tggcacaatt tctgtcgaat atgaagatgg tgaatcagac ttattaccca accaatttgt
     8641 attaattgca actggttctt cgcctgcaga gttgccattc ttatcatttg atcatgataa
     8701 aattttatct agcgatgata tattatcatt aaagacgtta ccatcaagta ttggtattat
     8761 tggtggtggt gttatcggaa tggaatttgc atcattgatg atagatttag gtgttgacgt
     8821 aacagttata gaagcaggtg aaagaatttt accaactgaa agtaaacaag cttcacaact
     8881 attaaaaaaa tcattgtcag cacgtggtgt taaattctat gagggaataa aactttctga
     8941 aaatgatata aatgttaacg aagatggtgt tacgtttgaa atttcttcag acataattaa
     9001 agtagataaa gtgttgctat cgattggtag aaaacctaac acatcagata ttggtttaaa
     9061 caacactaaa ataaaacttt ctacatcagg acatatttta acgaacgaat ttcaacaaac
     9121 tgaagataaa catatttatg cagctggtga ttgtatagga aaattacaat tggcacatgt
     9181 tggatcaaaa gaaggtgttg tggctgttga tcatatgttt gaggggaacc caatcccagt
     9241 aaactataac atgatgccta agtgtattta ttcacaacct gaaattgctt ctatcggttt
     9301 aaacattgaa caagcaaagg cagagggaat gaaagttaaa agttttaaag taccatttaa
     9361 agcaattggt aaagcagtga ttgatagcca tgacgcaaac gaagggtata gcgaaatggt
     9421 gattgatcaa tcaactgaag aaattgtggg tattaatatg attggtccac atgtaacaga
     9481 attgattaat gaggcatcac tgttacagtt catgaatggc tcggcattag aattaggact
     9541 aacaacacac gcacatcctt ccatctctga agtgttgatg gaattaggat tgaaagcaga
     9601 aagtagagct attcacgtat aaataggagg atttaacatg attgattata aatcattagg
     9661 ccttagcgaa gaagacctaa aagtaatata taaatggatg gatttaggta gaaaaataga
     9721 tgaaagactg tggttattga accgtgcagg taaaattcca tttgttgtaa gtggtcaagg
     9781 acaagaagca actcagattg ggatggcata cgcattagaa gaaggtgata ttacagcgcc
     9841 ttattataga gatctagctt tcgttactta tatgggtatc tcagcgtatg atacgttttt
     9901 atcagcgttt ggaaagaaag acgatgttaa ctctggtgga aaacaaatgc catcacactt
     9961 tagtagtaga gctaagaata ttttgtctca aagttcaccg gtagcaactc aaatacccca
    10021 tgctgttggt gctgctttag ctttaaaaat ggatggtaag aagaaaattg caacagcaac
    10081 tgttggtgaa ggtagctcaa accaaggtga cttccatgaa ggtttaaact tcgcgggtgt
    10141 acacaaactt ccttttgtat gtgtcattat aaataataaa tatgcaattt cagtacctga
    10201 ttcattacaa tacgcagctg aaaaattatc ggatagagca cttggttacg gtatacatgg
    10261 tgaacaagta gatggtaatg atccattagc aatgtataaa gcgatgaaag aagcaagaga
    10321 tcgagctatt tctggtcaag gttcaacttt gattgaagca gtaacaagtc gtatgacagc
    10381 tcattcatca gatgatgacg accaatatcg tacgaaagaa gagcgtgaag cgcttaaaaa
    10441 agctgactgc aatgaaaagt tcaaaaaaga gttgctttca gctggcatta tcgatgatgc
    10501 ttggttggca gaaatagaag cggagcataa agacatcatc aataaagcga caaaggctgc
    10561 tgaagacgcg ccatatccaa gtgtcgaaga agcctatgca tttgtgtacg aagaagggag
    10621 tcttaactaa tggctaaatt atcttattta gaggcgatac gccaggcaca agacttagca
    10681 ttacaacaaa ataaagatgt gtttattctt ggtgaagatg ttggtaaaaa aggtggcgta
    10741 tttggtacta ctcaaggact acaacaacaa tatggtgaag acagagttat cgatacgcca
    10801 ttagctgagt caaatattgt tggaactgcc attggtgcag ctatggtagg taaaagacca
    10861 attgcagaaa ttcaatttgc agattttatt ttacctgcga caaatcaaat tattagtgaa
    10921 gctgcgaaaa tgcgttatcg ttcaaataat gattggcaat gcccattaac gattcgtgca
    10981 ccatttggtg gcggtgttca cggaggacta tatcattcac aaagtattga aagtatattt
    11041 gcttcatcac caggtttaac aattgttatt ccttccacac cgtatgatgc taaagggctt
    11101 ttattatcat ctatcgaatc aaatgatcca gtattgtact ttgagcataa aaaagcttat
    11161 cgtttcttaa aggaagaagt gccagaagaa tattacactg tacctttagg aaaagcagat
    11221 gttaaacgtg aaggtgaaga tttaacagta ttttgttatg gattaatggt caattattgt
    11281 ttacaagcag cggatatttt agctgcagac ggtatcaacg ttgaagtcgt tgatttacga
    11341 actgtttatc cattagataa agaaacaatt attgatcgtg ctaaaaatac aggtaaagtt
    11401 ttacttgtaa ctgaagataa tttagaaggt agtattatgt cagaagtgtc agcgattatt
    11461 gcagagcatt gcttgttcga tttagatgca ccaatcatgc gtttagctgc tccagatgta
    11521 ccatctatgc cattttctcc tgtattagaa aatgaaatta tgatgaatcc agaaaaaatc
    11581 ttaaataaaa tgcgtgaatt agcagaattc tagggaggga aagtcatgga aataacaatg
    11641 cctaagttag gtgagagtgt tcatgaaggt accattgaac aatggttagt ttctgttggt
    11701 gatcatattg atgaatatga accattatgt gaagttatta cagataaagt gacagctgaa
    11761 gtcccttcca cgatatcagg aacaattaca gaaattttag ttgaagcggg gcagacagta
    11821 gctattgata caattatctg taaaattgaa actgctgatg aaaagacaaa tgaaacaact
    11881 gaagagatac aagcaaaagt ggatgagcat actcagaaat ctactaaaaa agctagtgca
    11941 acagtggaac agacatttac tgctaaacaa aatcaacctc gtaataatgg tcgcttttca
    12001 cctgttgtat ttaaactcgc ttcagagcat gacattgatt tatcacaagt tgtaggtagt
    12061 ggatttgaag gtcgtgtaac taagaaggat ataatgtcag ttattgaaaa tggtggtacc
    12121 acagctcaat ctgacaaaca agttcaaaca aaatcaacat cagtagatac atcaagtaac
    12181 caatcatctg aagacaatag tgaaaacagc acaataccag taaatggtgt gcgtaaagca
    12241 attgcgcaaa atatggttaa tagtgtaaca gagattccac atgcatggat gatgattgaa
    12301 gtagatgcta caaatcttgt gaatacgaga aatcattata aaaacagctt taaaaataaa
    12361 gaaggatata atctaacgtt ctttgctttc tttgtaaaag ctgtagcaga tgctttaaaa
    12421 gcatatcctt tattaaatag tagctggcaa ggaaatgaaa ttgtcttaca taaagacatt
    12481 aatatttcaa ttgctgttgc tgatgaaaat aaattatacg tacctgtgat taagcatgca
    12541 gacgaaaagt caatcaaagg tatagctaga gaaattaata ctttagcaac aaaagcgcgt
    12601 aataagcaat tgacaactga agatatgcaa ggtggaacat ttacagttaa taatactggt
    12661 acatttggtt cagtatcatc aatggggatt ataaatcacc ctcaagcagc gatattacaa
    12721 gtagaatcaa tcgttaaaaa gccagtagta attaatgata tgattgcaat tcgaagtatg
    12781 gtaaatttat gtatttcaat agatcatcgc attttagatg gtttacaaac tggtaaattt
    12841 atgaatcata ttaaacagcg tatcgaacag tatactttag aaaatacaaa tatatattag
    12901 tgataacatg gatacatcta tcgacaactt gttttatctt gttcttgtcg atggatgtat
    12961 ttttttgggc aataaaatat gtgcaataaa ttcaaaaaaa gataaagaac aataatcaac
    13021 atggttgaat gcatttttgc agtaaagtca aaataataca tcatactgga aacatattaa
    13081 tgaaaacagg tgaacaaatt agtttccatg atattaagca caataatgtt tggtatattg
    13141 ttaaaattgt gtctaaatat tggtgtgatt cagattagtt tattgaacaa tatgttatta
    13201 attagtagaa tgaggatagt ttaaatataa agggataggt gattgaactt atggacatga
    13261 atttcgattt atacatgaac ggtgttgtag aacaagcaag gaatgaaatt gaatctgcgg
    13321 gatatgagca attaactact gcagaagatg ttgacaaagt tcttaaacaa gatggtacaa
    13381 cactagttat gatcaattct gtatgtggtt gtgcaggtgg tatcgcaaga ccagcagcat
    13441 cacatgcttt acattatgac gtattacctg atcgtctagt gacagtattt gctggacaag
    13501 ataaagaagc gacacaaaga gcgcgtgaat acttcgaagg ttatgcgcct tcaagtccgt
    13561 catttgcatt agtaaaagat ggaaagatta cagaaatgat tgaaagacat caaatcgaag
    13621 gtcatgatgt gatgaacgta attaatcaat tacaaacatt attcaataaa tattgtgaag
    13681 aaagataaga ggcgctaacc catgttaaag ttaaatcctt acaagattgg atttagaaca
    13741 ataaaaacag cagtgggtat gactttaggt gtaattatta gtaagctgtt aggtttagat
    13801 aattatgctt caagcgccat attagtcgta ttatgtatta aacatacaaa agtacattcg
    13861 ctacaagcga ttatttcaag attagtatca tgttttttag tattgttttt aggttcagca
    13921 atatttagtt tattaggtca gagtccaatt gtactcggta ttatcgtatt gttatttata
    13981 ccattaactg tcgtattaaa agtacaagaa ggtgtcatta cgagttgcgt tatattactt
    14041 catgttttta atgcaaaatc aattgatgca catttaattg ttaatgaaac attattactg
    14101 ttaattggac tgagcattgc atttacaatg aatttaatga tgccaagttt agacaaacaa
    14161 ctagacgaat acaaatgtaa aattgagcaa caaattgctg atatttttag taaatatagt
    14221 tatatttgtg aaaaatatga agataccatt gcgattgaat ttgaagtgtt acttttaaat
    14281 attaaaaagg cgaagtctat cgcgttccga gatgttaaaa atcattttgt tagaaacgaa
    14341 aattcatact atcattattt tgatatgcga gaagagcaag tggaattgtt aatgagaatg
    14401 aaaccgctca tcgaaagtat ctgtcataaa gatccacttt taaatgatgt ttcgtcactg
    14461 ctgaaagaaa ttggtctaaa tgtgaatagt aatgactata cagcaatgcg tttacacaat
    14521 ttgtatgaga cgcgtttgaa attggatgaa ctgccattgc caacaacaca caaagcgctt
    14581 aactcgagag caagtattat tcaaatatta aatgaattag aagaatattt acaaatcaaa
    14641 tcgcaatttg gttcattgaa aatgcatagt gaagtagctt aaaaaataat ccatccgtat
    14701 gacccatgtt tgaaagtaat cgttgcaata cgataactta ttaatacatg cgtttataac
    14761 ggatggattt ttaattgtat gattttgtat aggtagttga gatatagttt ataaaattgg
    14821 aatgatactc gttaataaca atgctaatac aactgataca agcataacga taatgataat
    14881 tcttaaaact ttattttgca ttttgtgtgc ctccaaatga ataacctttg tattaataat
    14941 tacgacataa gatataaaac ttttaacttg agacgatata actagcaatc atcattaagg
    15001 aatataataa aaaacatgag taagtttgct tatgtcattt tcctcattat acgatatctt
    15061 tgaaaaatca atcaatatat ttttgacaaa gcttagtttg agttacaata aatatatata
    15121 ataaattgaa aataatatga cacaatttac aagcctgata aatctgtgta acgtcaatta
    15181 ctgtaacatt ttatttcatc aatttttacc gtttggcgta cttgaaaatt ttgataaaag
    15241 gggttccatt atgattaatg agcaaagatt acttaatacg tttttagaac ttgtacaaat
    15301 cgattctgag acagggaatg aatcaacaat ccaacctatt ttaaaagaaa aattcatagc
    15361 attagggtta gatgttaaag aagatgaagc ggctaaacat cctaaattag gtgccaataa
    15421 tctagtgtgt acaatgaata gtactatcga agaaggcgaa gtacctaaat tatatttgac
    15481 gagccatatg gatactgttg tacccgcaat taatgtaaag ccaattgtaa aagatgacgg
    15541 ctacatatac tctgatggta cgactattct aggtgcagat gataaagcag gattagcagc
    15601 gatgcttgaa gttttgcaag tgataaagga acaacaaatc ccacatggac aaattcaatt
    15661 tgtgattact gttggggaag aatcaggttt aattggtgct aaagaattaa attcagagtt
    15721 gttggacgcg gactttggtt atgctattga tgcaagtgct gatgtcggta ctacggttgt
    15781 aggtgcaccg acgcaaatgt taatttcagc taaaattatt ggcaaaacag ctcatgcaag
    15841 tacgccaaaa gagggtgtta gtgctattaa tattgcggct aaagcaatta gtcgaatgaa
    15901 attaggacaa gttgatgaaa taactactgc caatattggt aagtttcatg gcggttcagc
    15961 tacgaatatt gttgcagatg aagtcatttt agaagcagag gcacgttctc acgatccaga
    16021 aagaataaaa acacaagtca aacatatgac tgatgtgttt gaaactactg catcagaatt
    16081 aggtgggaaa gcagaagtta cagtggagca aagttatcca ggatttaaaa ttaatgataa
    16141 tgaagcggtt gttaaaattg cacaagaaag tgctcgtaat ttaggattat ctgcaaatac
    16201 aattatttct ggtggcggtt cagacggcag tattattaat acatttggta ttccttcagt
    16261 cattttagga gtgggttatg aaaaaatcca tacaacaaat gagagaatgc cgattaaatc
    16321 attgaacttg cttgcaagtc aagtgttaga aataatcaaa atagttgcgc gacaatcgaa
    16381 atgatttagt gtagtgtatg tctattttgt gatacaatac taatgaaaaa tcaacaagtg
    16441 aggtaagtaa tatgacacaa caaattggag ttattggttt agccgttatg ggtaaaaacc
    16501 tagcttggaa tattgaatca cgcggatata gtgtatctgt atttaaccgc tcaagtgaaa
    16561 aaactgattt aatggttgaa gaatcaaaag ggaaaaatat tcatccaacg tattcattgg
    16621 aagaatttgt taattcttta gaaaaaccac gtaaaatttt attaatggtt caagcaggta
    16681 aagctacgga tgcaacaatt gatagtttgt tacctttatt agatgatgga gatattttaa
    16741 ttgatggtgg taatacaaac tatcaagata ctatcagacg taataaagca ttagcacaaa
    16801 gtgcaatcaa ctttattggt atgggcgttt ctggtggtga aattggagcg ttaactggtc
    16861 cgtcattaat gccgggtgga caagaagaag cttataacaa agttgcagat attcttgatg
    16921 caattgctgc taaggcaaaa gacggtgctt catgtgttac ttatattgga ccaaatggtg
    16981 ctggacacta tgtgaaaatg gtacataatg gtattgaata tgctgacatg caattaattg
    17041 cagaaagtta tgctatgatg aaagaattat taggtatgtc tcatgaagac attgctcaaa
    17101 cgttcaaaga ctggaatgct ggtgaattag aaagttattt aattgaaatc actggtgata
    17161 ttttcatgaa attagatgaa aataaagaag cattagttga aaaaattctt gatactgcag
    17221 gtcaaaaagg tacaggaaaa tggacttcaa ttaatgcatt agaattaggt attccattaa
    17281 caatcattac tgaatctgta tttgcacgtt ttatctcttc aatcaaagaa gagcgcgtaa
    17341 atgcttctaa agaattaaat ggtccaaaag catcatttga tggtgacaag aaagactttt
    17401 tagaaaaaat tcgtaaagca ttatatatga gtaaaatttg ttcttatgca caaggtttcg
    17461 cgcaaatgcg taaagcaagt gaagataatg aatggaattt aaaacttggt gatttagcta
    17521 tgatttggag agaaggttgc attatacgtg cacaattctt acaaaagatc aaagatgctt
    17581 acgataataa tccaggatta caaaacttat tgctagatcc ttactttaaa aacatcgtta
    17641 ctgaatatca agatgcgtta cgtgatgttg tcgcaactgg tgttcaaaat ggtgtaccaa
    17701 cacctggttt ctcatcaagt attaattact atgatagtta ccgtgcagct gatttacctg
    17761 caaacttaat tcaagcacaa cgtgactact tcggtgctca cacgtacgaa agaaaagata
    17821 aagaaggcgt attccataca caatggattg aagaataata ttgaccattg agcctgagac
    17881 cttatgaatt taggtaatgt taaagagaca atttctatta tttatttgat agaaattgtc
    17941 tctttcctta tgtatttatc gaaattttct acgcatgata tattcaatga ttttctttct
    18001 cttgtcaaat taaaattttc aacttttata aattaacgat atctatcctc atagaatttg
    18061 tccgtttaag tgtagacgtt ttacttacat actattaaga tagcattagt aaataatttt
    18121 aatatttcga taaatagatt acgttttatt atttgatgaa taagagggat agaatgtcat
    18181 atacttctgt attgtttaat aagaacttta ctttttattt aatagggata gctttttcta
    18241 acatgggcag tgcttttact acgtttgttt ttccattaat gattttaaag cttactggat
    18301 ctgcatttca agttggcata gtttctgcac taagttttat accttatgca attttaggat
    18361 tgccagctgg tgctttaatt gacagattga ataagaagac cataatgaaa tgtgctgata
    18421 taattaggtt cgtttcatat ttaagtatac cagtattaag ttttttcaat atgttaagca
    18481 tctttcaaat ttatgtagtt gcaatcattt caggtattgg attagtattt cacagtattt
    18541 ctgaggtttc gataatccca agtattgtaa aagaagaaga tttggcgagc gcaaacagtt
    18601 atatatacgc tactcaaaat gtttcggaat ttttgggccc tataattgga ggtttacttt
    18661 atacatatat gggatattca atattgattt ttatagattc tatgactttt ttactctcat
    18721 ttttcagttt gatattaatt aaaattgaaa caaagagtat attcaatcaa gaaaagttga
    18781 gtggaaaaaa ttttttgagc gatgttaaag ttggatttga ttatctatta tctaattcaa
    18841 ctattagagt aatggctgtt gttgtaagtt tatccaatct aattatctca ccttattata
    18901 tttatatagt aatgtttgta aaggaagaca tgaatcaatc atctcaagca ttggggttag
    18961 ttttaggtat ctcatcatta ggtgcgctaa tcggttcatt atctgcaagt tttctcttga
    19021 aattatttaa tttcggaaaa ctaattgtaa ttatattatt tttagataca atttttagat
    19081 taatgttacc atttagtact tatattttta tactcatccc acttttaggg ttgacatata
    19141 tgacacaatc cattttaaat atagcgataa tcacactgag gcaaaagaaa tgttcagaaa
    19201 atatgttagg acgtgttaat agtgttttta aaacaatggt ttttgcattc agacctatag
    19261 gccttttttt aggcggtata ttacttgaaa ataaaggtgg attttatgca cttacgatca
    19321 gtgctgtgtt atttatacca ttagtgttat atatactgaa aaatagattt tatcaagttg
    19381 aagaatatta agaagaaaat taataaaaat ggctgctatg tcaaattgga ctgatgaatt
    19441 caatatagga agttaggcaa ctaaacattg tataactttc tttttagtta ttggagctta
    19501 aagttttgaa cataataata ttcgattgcg caaatgattg taacttccat aatcaaatga
    19561 tgtacgttta attaatttta ttatgttagt tatacattct aatggacaat gtgagctaca
    19621 cccagttaag ttgacataga aaatacgaaa actttgaacg aagtatttca ttgatatatg
    19681 tcccagacga catgtttgat taatgaagtt ctatgtggct acaaaatttt aaaaattaca
    19741 caagcaatat ctattacctt aaaagcatcc cgccatattt ttaagcattt tgtgaagtaa
    19801 taaaatattt actgtgttat gatagcatgt actatagaga aatagaggta ctattttatg
    19861 gaaaagttaa atcaagttat gttatatgtt gatgatgttg aaaaagcgaa agcattttgg
    19921 acagaaactt tagaatttgt tgttgtaagt gaaacaccgt tagctgagga ctatgttgca
    19981 gttgaagtat cacctaccaa agatgctgaa acgtcattga cgattatggc aaaagaattt
    20041 attgaaaagc atagtccaga ggtgaaccta ggaacaccat ctttaatgtt taaagagaaa
    20101 aactttgatg cattatactc aaaattaaat gatttaggtt taactgggca tgatatagta
    20161 gaaatggatg gtcaacgtgt atttaatttc caagatggac aaggtaatta ttttgcagtt
    20221 agtgactaaa caatcgattt taatatttat acaaaccttt atattgattt tcggattgaa
    20281 tttcagtatg aaggttttat ttttatcgtt ttatgcaatc gtttgcacaa atcttttgac
    20341 ttttttagaa agagtaacta aactgttatt atatagaggt tggaagcgtt ttccaatgca
    20401 gaaattaaca ggaggtgatt aaatggttac gattaaagat gttgcactaa aagccggtgt
    20461 ttctccttcg acagtttcaa gagttataaa aggaaataaa cgtattagcg aagcgacaat
    20521 ttcaaaagtg aagaaagtta tggaagaatt gaattatttt cctaataccg ctgctagaac
    20581 tttaattaca aaccaaacat ataaaattgg tttagtgtta aaagggtctg aggagcctat
    20641 tcgactgaat ccattctaca tcaatgtatt gctaggaatt tctgaaacgt gtaaccagca
    20701 tggctatggt acacaaacga cagtctcaaa taatatgaat gatttaatgg atgaagttta
    20761 taaaatgatt aaacaacgaa tggttgatgc gtttatactg ctttattcaa aagaaaatga
    20821 tccaattaaa caaatgttaa ttgatgaaag catgccattt attgtgattg gtaagcctac
    20881 atcggatata gatcatcaat ttacacacat agataatgat aatatattag cttctgaaaa
    20941 tttgacacga catgttattg aacaaggtgt agatgaatta atatttatta cagaaaaagg
    21001 aaattttgaa gtttcaaaag atagaattca aggatttgaa acggttgcat cacaatttaa
    21061 tctggattat caaattattg agactagtaa tgaaagagaa gttattttaa attacatgca
    21121 aaatctacat acgcgtttga aggacccaaa tattaaacag gcaatcattt cgttagatgc
    21181 tatgttacat ttagcgattt taagtgtcct atatgaactt aatattgaaa ttccgaaaga
    21241 tgtaatgaca gcaacgttca atgattctta tttaaccgaa attgcgtcac cacctcaaac
    21301 ctgtattgac atcaagcctc gaatgttagg tcaacaggct ggttcagcaa ttttaaatat
    21361 attgaaaaac aaagcacagg atgttattga actcgtcatt atagatacag aattaaaaat
    21421 aagaaaatca acacagcgat agcaaaggag ttttaaaatg aataagcaat ggtggaaaga
    21481 agcagtagca tatcaagtat atccaagaag ttttaatgat agtaatcacg atggtattgg
    21541 ggatttacct ggaatgattg ataaattgga ctacttaaaa gatttaggta tcgatgtcat
    21601 ttggctcagt ccaatgttta aatcacctaa tgatgacaat ggttatgata ttagtgacta
    21661 tcaagaaatt atggatgaat ttggaacgat ggaagacttt gattgtttat taaaaggtat
    21721 tcatgataga ggcatgaagc ttattttaga tttagttgta aatcatacat ctgatgaaca
    21781 tccttggttt atagaatcta aatctagtaa agacaatccc aaacgtgatt ggtatatttg
    21841 gcaagatcca aagccagatg gctctgaacc taacaactgg gaaagtatct ttaatggatc
    21901 tacatgggaa tatgatgcta atactgagca atattatttc catttattca gtaaaaaaca
    21961 acctgatttg aattggggta atccagaagt tagagatgct gtatttgaaa tgatgaactg
    22021 gtggtttgat aaaggcattg atggatttag agtagatgca attacgcata ttaagaagac
    22081 gtttgaagcg ggtgacttac ctgtacctga gggtaaaaca tatgccccag catttgatgt
    22141 agatatgaat cagccaggta tacaaacttg gttacaagag atgaaagatc gctcattaag
    22201 taagtatgac attatgactg tcggtgaagc gaatggtgta agccctgatg atgctgatga
    22261 ctgggtcggg gaagaaaatg gtaaatttaa tatgatattc caatttgaac atttgggact
    22321 gtggaatagt ggtgattctc actttgatgt aaattcgtat aaatctgtat taaatagatg
    22381 gcaaaaacaa cttgaaaata aaggttggaa tgcgttgttt attgaaaatc acgaccaacc
    22441 acgacgtgta tcgacgtggg gtgacgatga caagtattgg tatgaatcag caacaagtca
    22501 tgcagctgtt tatttcttgc aacaaggtac gccattcatt tatcaaggtc aagaaattgg
    22561 tatgacgaat tatccatttg aaagtattga aacgtttaac gatgttgctg taaaaaatga
    22621 ctatcaaata gtgaaagctc aaggtggaga tgtagacgct ttacttgcga aatataaaga
    22681 tgagaaccga gataattctc gcacaccaat gcaatgggat gatacgttaa atggaggatt
    22741 tacaaatggt gaaccgtggt tcccagtgaa tctgaattat aaaactatca atgttgcaca
    22801 acaattagaa gatgagcatt cagtattaca attttataaa gatttaattc aattaagaaa
    22861 gtctaatgat gtatacgtat atggtcaatt tgatttagta gatgctgaaa attcacaagt
    22921 ttttgcgtac acgagaacat taaatgaaaa gcaagttctt attgtaggta atcttactaa
    22981 ccacgaagca gaattaactg taccatttga tttaagccat ggagaagtaa agctatttaa
    23041 ttatgatgcc aaagttaatt taaaacagtt acgtccatat gaagcatgtg ttatcgaact
    23101 aaattaacat attaatatgc cccct
//
LOCUS       DAOBBH010000024        22094 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.024, whole genome shotgun sequence.
ACCESSION   DAOBBH010000024 DAOBBH010000000
VERSION     DAOBBH010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22094
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.024"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..326
                     /gene="lpl2"
                     /locus_tag="P6L01_000673"
     CDS             <1..326
                     /gene="lpl2"
                     /locus_tag="P6L01_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl2"
                     /protein_id="HDP3029761.1"
                     /translation="FKFFGQYADFKSLKSYNHGDVSINSNVPSYDAKFKMSNKDENVK
                     QLRSRYNIPTDKAPILKMHIDGDLKGSSVGYKKLEIDFSKEENSELSIVDSLNFQPAK
                     NKDDE"
     gene            345..1127
                     /locus_tag="P6L01_000674"
     CDS             345..1127
                     /locus_tag="P6L01_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDP3029762.1"
                     /translation="MGYSKRFALYISILILIVMVAGCGKSDETKEDSKEEQIKKSFAK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWVLYSAIVSQPKGESLKSRGMILKLDR
                     NKRTAKGSYIIRELKEDKNHDVQKNEKKYPVKLVNNRIVLVKDVKDKKLKNEIESFEL
                     FSQYGNFNHFDRNEITNISYNPNAPNYSAEYKMKKNDRNIQQLKKRFNLKTSKTPKLL
                     FKGSGDIKGSSVGYKEIEIIFSRSKEEAFIMLTALSSFQVTK"
     gene            1187..1978
                     /locus_tag="P6L01_000675"
     CDS             1187..1978
                     /locus_tag="P6L01_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001802015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDP3029763.1"
                     /translation="MGYLKRLVLYIVIMVMSVFIIGCDKSSDTAENPKEGSKEAQIKK
                     SFSKTLDMYPIKNLENLYDKEGYRDGEFKKGDKGTWTISTDFAKSNKQGEMNSEGMVL
                     HFNRNTRTATGYYTVRTTYDEVDKLAREKKYRVEFKNNKIVLLDKVEDENLKQKIENF
                     KFFGQYADFKDLKNYKNGRISSNENVPYYEAEYKRNNSDGNVKKLREKYPITTKQSPI
                     LKLHIDGDIKGSSVGYKQIEYTFSKEKDDETFMSDFLNFGPSHSK"
     gene            2030..2830
                     /locus_tag="P6L01_000676"
     CDS             2030..2830
                     /locus_tag="P6L01_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDP3029764.1"
                     /translation="MGYFKRVVLYIIVMVVSVFIIGCNKSSDTSEKPKEDSKETQIKK
                     SFAKTLDMYPIKNLEDLYDKEGYRDGEFEKGDKGMWTIYTDFAKSNKPGELSNEGMVL
                     YLDRNTRTAKGYYFVRTFYRKDKLPDRKNYKVEMKNNKIILLDKVEDPNLKKRIENFK
                     FFGQYANLKELKNYSNGDVSINENVPSYDVKYKMSNKDENVKQLRSRYNIPTDKSPVL
                     KMHIDGNLKGSSVGDRKLEIDFSKRENSHLSVIDSLDYQPAKVDEDER"
     gene            2820..4145
                     /locus_tag="P6L01_000677"
     CDS             2820..4145
                     /locus_tag="P6L01_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001581953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029765.1"
                     /translation="MKDKYKIDPGIIKNNTEETTAISKISYEVENANLYGADSEDITR
                     QIEYLKAKKKFPSNLEYVDSYTDSLNGVTTSAFLNKDTGKVTLGMTGTNIQGEAFKKL
                     KEGEFSRQNVTNALETVKDGYADLKILYSPASDQNYRYANTQEFINKIKSKYDIDFIT
                     GHSLGGRDAVVLGMSNGIPNIVVYNPAPISINSLNPNSPDGKRLLELYKNYKGNITRF
                     VAENDALTENLKKYKHYVFFGNDKVFKNGKGHEMEGFLTEEEQKAIKKELKKLQGYAE
                     ENNKSFVKNSNNAISKLASIELLRANMMTTNGGGLSSSQQKVLESLTALTIAQSFSQL
                     IDDEINQIKKMYNEKKKEFGKNWEDAQKAGKAVGEDLSVNGVLNALDEGQVNESSMVR
                     EPEQMISAKERQLSTIGSSVSNYIMRVRLSINEIVDKDQVLASQIGGLL"
     gene            4142..4465
                     /locus_tag="P6L01_000678"
     CDS             4142..4465
                     /locus_tag="P6L01_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029766.1"
                     /translation="MMFNQINNKNELEESYESEKKRIENELHNLNELRHRTRKENERS
                     YDVFQYLKHEMNYSEDAQRKMTRNIEAYEQEINEIIRKQEWKLEEYKEDLKKSYEKQL
                     DKLSD"
     gene            4484..4798
                     /locus_tag="P6L01_000679"
     CDS             4484..4798
                     /locus_tag="P6L01_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029767.1"
                     /translation="MTLIEPDMNLRMPDISTTVETLNLISKMEAQKENIRSVIAPEHK
                     HKYKDIENGLKGEEKVLIEQMAQHCEAFKANFKGAAQGDWVKSAMSEIDSIKDDLKKI
                     NS"
     gene            complement(5087..5287)
                     /locus_tag="P6L01_000680"
     CDS             complement(5087..5287)
                     /locus_tag="P6L01_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029768.1"
                     /translation="MNMLFVILVLYVIGIAFILLSVFGSKTEGLSTKHTLYTIGSAII
                     TIAIFISIGYAIQYLTAALYGL"
     gene            5466..6668
                     /locus_tag="P6L01_000681"
     CDS             5466..6668
                     /locus_tag="P6L01_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDP3029769.1"
                     /translation="MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVN
                     IDKDLVADGGGLSRTDEKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGI
                     SEPVPVAQTFSYIDDELGIDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDE
                     TDERSIADLLIDQVEFCDVLIINKIDLISEEELAKLEKVLSALQPIAKIIKTTNSEVD
                     LKEVLNTQRFDFEKASESAGWIKELESGGHASHTPETEEYGISSFVYKRRLPFHAKRF
                     NDWLESMSNNVVRSKGIVWLAQYNHVACLLSQAGSSCNIHPVTYWVASMSEAQQTQIL
                     AERQDVAAEWDPEYGDRHTQFVIIGTELDEEKLTKELDACLVNAQEIDADWQQFEDPY
                     QWQIRPAR"
     gene            complement(7327..7389)
                     /locus_tag="P6L01_000682"
     CDS             complement(7327..7389)
                     /locus_tag="P6L01_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-4"
                     /protein_id="HDP3029770.1"
                     /translation="MAIVGTIIKIIKAIIDIFAK"
     gene            complement(7453..7521)
                     /locus_tag="P6L01_000683"
     CDS             complement(7453..7521)
                     /locus_tag="P6L01_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-3"
                     /protein_id="HDP3029771.1"
                     /translation="MEFVAKLFKFFKDLLGKFLGNN"
     gene            complement(7573..7638)
                     /locus_tag="P6L01_000684"
     CDS             complement(7573..7638)
                     /locus_tag="P6L01_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-2"
                     /protein_id="HDP3029772.1"
                     /translation="MGIIAGIIKFIKGLIEKFTGK"
     gene            complement(7670..7735)
                     /locus_tag="P6L01_000685"
     CDS             complement(7670..7735)
                     /locus_tag="P6L01_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-1"
                     /protein_id="HDP3029773.1"
                     /translation="MGIIAGIIKVIKSLIEQFTGK"
     gene            8256..9740
                     /locus_tag="P6L01_000686"
     CDS             8256..9740
                     /locus_tag="P6L01_000686"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit 5"
                     /protein_id="HDP3029774.1"
                     /translation="MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVM
                     PVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHY
                     FPLFTAITSFASLAWMSEDLRLMALCWGMTLLCLTLLMNVNRFWKVPRESAKLSSMTF
                     LCGWLAFVGAIVTIYIATGEWRVPQHIVHPTWSLLTNVLLVLAVMIPAAQFPFHRWLI
                     ESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLV
                     QVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNI
                     PKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILAWSLLVSWNQMVAF
                     SKGRMARLVGMILIAIVTFIYVITHNYFYDVLQNITTHATTPPTVSVIISVVILIFGS
                     LLSIWVARHRYSKGFAVLYVWLVNLGEARSKAIESHPNYLKKYL"
     gene            9753..12458
                     /locus_tag="P6L01_000687"
     CDS             9753..12458
                     /locus_tag="P6L01_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcC family protein"
                     /protein_id="HDP3029775.1"
                     /translation="MTTQLNINSVIENAKRVITPLSPISIFAARNPWEGLEADTFEDV
                     AKWLRDVRDVDIFPNKALIESAVARGELDESVFNQLVTDMLLEHHYNIPQHYINLYID
                     NIKTLKDVPASYMNHSNVDVVADLLLEKSKRDMAESYHHYDVRPMSDAIIDEQGEPLS
                     EQVNRQMIKWTKLYIDQFLSSWTMPKREQSFYHAWLHLAQHDHSFTKAQRQVIKGLPN
                     DPEMTIESVLTYFSIDQEDYQAYVEGHLLALPGWAGMLYYRSQQHHFEQHLLTDYLAI
                     RLVVEQLLVGDEFKSVTKDCESRSENWFKQTVASLCYYSDMPSDVLLQHDVNEIQTFI
                     HFAATMNKNVFKNLWLIAWEMTYESQLKQKIKAGHESVAGALDVNQVNVSENDNANQP
                     HSVLLNDTQAVDENNSELNQVGTSTKAQIAFCIDVRSEPFRRHIEAAGPFETIGIAGF
                     FGLPIQKDAVDEQFKHDSLPVMVPPAYRIKEFADRYDMNVYRQQQQTMSSMFYTFKLM
                     KNNVMPSLLLPELSGPFLSLSTIVNSIMPRKSRASLQKIKQKWLKKPETKLTIDREFD
                     RTSDLPVGFTEQEQIDFALQALKLMDLTEAFAPFVVLAGHASHSHNNPHHASLECGAC
                     GGASSGFNAKLLAMICNRPNVRQGLKQSGVYIPETTVFAAAEHHTSTDTLAWVYVPDT
                     LSALALDAYESLNDAMPMISEQANRERLDKLPTIGRVNHPVEEAQRFASDWSEVRPEW
                     GLAKNASFIIGRRQLTKGIDLEGRTFLHNYDWRKDKDGKLLNTIISGPALVAQWINLQ
                     YYASTVAPHFYGSGNKATQTVTSGVGVMQGNASDLMYGLSWQSVMAADRTMYHSPIRL
                     LVVIQAPDYVVARLFANNEHFARKVSNHWLRLMSVNEEGRFKSWI"
     gene            12620..12982
                     /locus_tag="P6L01_000688"
     CDS             12620..12982
                     /locus_tag="P6L01_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HDP3029776.1"
                     /translation="MKRTKGEIEAEISKAITQWEKDFLGRGSLSVKSDILRDMVIISL
                     QGILTPAEYRVCSTNEGLLNIKRTRSELVESGEQDLNDIILKITGIKVMSFHSDLSTV
                     TGERIIVFKLEDNLEKHI"
     gene            complement(13211..13627)
                     /locus_tag="P6L01_000689"
     CDS             complement(13211..13627)
                     /locus_tag="P6L01_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029777.1"
                     /translation="MLSIISMCHKFHFTDNIKIIKFILLCILQIKHCIIEITIFCYFQ
                     FSTIYIYYTEGVRRINRVIFVYIALIITLVRFFTPIHPSFSNLIYWIFVLYFIPIILC
                     VIGFKAEKLIATMVIIPNFLGILYRLYAYVTHILFM"
     gene            13665..14339
                     /locus_tag="P6L01_000690"
     CDS             13665..14339
                     /locus_tag="P6L01_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDP3029778.1"
                     /translation="MIDKKLTSPKMTVPLFLIALIVFIGMFYGVVTNQEWLKNIDMGS
                     LTWFTDYFGEPQRQYVNNLFNYYMTFSAEIGDVKGVVLISIIVTIILFIKQRHLAVWF
                     VTYLVSGVIMNKLIKDTVLRPRPYNHLAVDTGFSFPSGHSNASTLLYFALMIIIISVA
                     AKTITKVLSALVMGILWLSILFCRLYFHAHYFSDVIGGTSLAIIWVALFLMVYPYFIN
                     HRRQRV"
     gene            14376..15110
                     /locus_tag="P6L01_000691"
     CDS             14376..15110
                     /locus_tag="P6L01_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HDP3029779.1"
                     /translation="MRIKTPSPSYLKGTNGHAILLLHSFTGTNRDVKHLAAELNDQGF
                     SCYAPNYPGHGLLLKDFMTYNVDDWWEEVEKAYQFLVNEGYESISATGVSLGGLMTLK
                     LAQHYPLKRIAVMSAPKEKSDDGLIEHLVYYSQRMSNILNLDQQASSAQLAAIDDYEG
                     EITKFQHFIDDIMTNLNVIKIPANILFGGKDEPSYETSAHFIYEHLGSVDKELNGLKD
                     SHHLMTHGEGRDILEENVIRFFNALT"
     gene            15482..16819
                     /locus_tag="P6L01_000692"
     CDS             15482..16819
                     /locus_tag="P6L01_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDP3029780.1"
                     /translation="MKRQQSQWKSSTGFILASAGSAIGLGAMWKFPYMAGIYGGGAFL
                     AMFLIFTIFVGLPLLIMEFTVGKMGRTYTTQIYSKLTGKKWLNIIGWNGNLAVFILFG
                     FYSVIGGWIVIYIGQVLWQLVIFQRINHLQEMNFEAVISNPWLTVLGQGIFIFATMII
                     VMLGVEKGLEKASKVMMPLLFVFLIIIVIKSLTLDGALEGVKFILQPRVSEITADGIL
                     FALGQSFFTLSLGTTGMITYASYASKDMTIKSSAISIVVMNIFVSVLAGLAIFPALHS
                     FGYEPQEGPGLLFKVLPMVFSQMHLGTLFYLGFLVLFLFAALTSSISLLELNVSNFTK
                     NDNTKRKKVAVIGSILVFIISIPATLSFGILKDVRFGAGTIFDNMDFIVSNVLMPLGA
                     LGTTLVIGQLLDKKLLQQYFGKDRFRLFNGWYYLIKYAMPVVIILVFIVQLFS"
     gene            17036..17941
                     /locus_tag="P6L01_000693"
     CDS             17036..17941
                     /locus_tag="P6L01_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDP3029781.1"
                     /translation="MITYDLIGNTPLVLLEHYSDDKVKIYAKLEQWNPGGSVKDRLGK
                     YLVEKAIQEGRVRAGQTIVEATAGNTGIGLAIAANRHHLKCKIFAPYGFSEEKINIMI
                     ALGADVSRTSQSEGMHGAQLAARSYAEKYGVVYMNQFESEHNPDTYFHTLGPELTSAL
                     QQIDYFVAGIGSGGTFTGTARYLKQHHVQCYAVEPEGSVLNGGPAHAHDTEGIGSEKW
                     PIFLERRLVDGIFTIKDQDAFRNVKSLAINEGLLVGSSSGAALQGALNLKAQLSEGTI
                     VVVFPDGSDRYMSKQIFNYEENDYE"
     gene            17934..19076
                     /locus_tag="P6L01_000694"
     CDS             17934..19076
                     /locus_tag="P6L01_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional cystathionine
                     gamma-lyase/homocysteine desulfhydrase"
                     /protein_id="HDP3029782.1"
                     /translation="MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYE
                     YSRTANPTRSSVESVIAALENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGT
                     YRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
                     AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAE
                     RLAFISNSTGGILGPQDSYLLVRGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHP
                     SIESHLNHDVHMAQADGHTGVIAFEVKNTESAKQLIKATSYYTLAESLGAVESLISVP
                     ALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL"
     gene            19371..20396
                     /locus_tag="P6L01_000695"
     CDS             19371..20396
                     /locus_tag="P6L01_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDP3029783.1"
                     /translation="MIEFRQVSKSFHKKKQTIDALKDVSFTVNRNDIFGVIGYSGAGK
                     STLVRLVNHLEAASNGQVIVDGHDITNYSEKGMREIKKDIGMIFQHFNLLNSATVFKN
                     VAMPLILSKKSKTEIKQRVTEMLEFVGLSDKKDQFPDELSGGQKQRVAIARALVTNPK
                     ILLCDEATSALDPATTASILTLLKNVNQTFGITIMMITHEMRVIKDICNRVAVMEKGQ
                     VVETGTVKEVFSHPKTTIAQNFVSTVIQTEPSTSLIRRLNDEQVGGFKDYKIFVEETQ
                     VTQPIINDLIQICGREVKILFSSMSEIQGNTVCYMWLRFNIDQQFDDTAINQYFKEKN
                     IQFEEVH"
     gene            20400..21059
                     /locus_tag="P6L01_000696"
     CDS             20400..21059
                     /locus_tag="P6L01_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3029784.1"
                     /translation="MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITR
                     KQGIWPNIVIHQVLNPLINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYV
                     APYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLI
                     GSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVIIVQVIQTLGNVLARFIRR
                     H"
     gene            21096..21938
                     /gene="gmpC"
                     /locus_tag="P6L01_000697"
     CDS             21096..21938
                     /gene="gmpC"
                     /locus_tag="P6L01_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter
                     glycylmethionine-binding lipoprotein"
                     /protein_id="HDP3029785.1"
                     /translation="MKRLIGLVIVALVLLAACGSNNDKKVTIGVASNDTKAWEKVKEL
                     AKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTV
                     LAPLGIYSDKIKDVKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVK
                     DITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSNSDAV
                     KPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIET
                     SLAK"
BASE COUNT         7695 a         3137 c         4396 g         6866 t
ORIGIN      
        1 actttaaatt tttcggacaa tatgccgatt tcaagagttt gaaaagttac aaccatggcg
       61 acgtttcaat taatagtaat gttccaagtt atgacgcgaa atttaaaatg agtaataaag
      121 atgaaaatgt taagcaatta agaagccgtt ataacattcc tactgataaa gctccaatat
      181 taaaaatgca tattgatggg gacttaaaag gcagttccgt tggatataaa aagttagaaa
      241 tagacttttc aaaagaagaa aatagcgaat tatcaatagt cgattcatta aattttcaac
      301 ctgccaaaaa taaagatgat gaatgatagg gaggatgtga agcgatggga tattcaaaaa
      361 ggtttgcatt gtacataagt atcttgattt taatagttat ggtagcaggt tgtggtaaaa
      421 gtgatgaaac aaaagaagat tccaaagaag aacaaattaa aaagagcttt gcgaaaacgt
      481 tagatatgta ccctattaag aatctcgagg atttatacga taaagaagga taccgtgatg
      541 gcgaatttaa aaagggtgat aaggggacgt gggttctgta ttcagctata gtatcacaac
      601 caaaaggcga gagtttaaaa tcacgaggaa tgatattaaa gttagataga aataagagaa
      661 ctgctaaagg aagttatatt attagagaat tgaaagaaga taaaaatcat gatgttcaaa
      721 aaaatgaaaa gaaatatcca gtgaaattgg tgaataatag gatagttttg gtaaaagatg
      781 ttaaagacaa aaagttaaaa aatgaaatag agtcgtttga attattttca caatatggaa
      841 actttaatca ttttgatcgg aatgagatta ctaatatttc atataatcct aatgctccca
      901 attactctgc agaatataaa atgaagaaaa atgacagaaa cattcaacag ttgaaaaaga
      961 gatttaatct aaaaactagc aagacaccaa aattattgtt taagggatct ggagatataa
     1021 aggggtcttc tgtaggatat aaggaaatag aaatcatatt tagtagaagt aaagaagaag
     1081 catttattat gttgacagcg ttgagttcgt tccaagtaac aaaatgacaa aaagttgata
     1141 ttagtcaaca aaagcaaata ttgagaataa agagagttat gaaatgatgg gttatttaaa
     1201 aagacttgta ttgtacatag ttattatggt tatgagtgtt tttataatag gttgtgataa
     1261 atcaagcgat actgcagaaa atccaaaaga aggttcaaaa gaagcacaaa ttaaaaagag
     1321 tttttcgaaa acgttagata tgtatccaat taagaatctc gagaatctat atgacaaaga
     1381 aggatatcgt gatggcgaat ttaaaaaagg tgataaaggg acttggacaa tatcaacaga
     1441 ttttgctaaa agcaacaagc aaggtgaaat gaatagcgaa ggtatggtac tacattttaa
     1501 tagaaatacg aggacagcaa caggatatta tactgtaaga acaacttatg atgaagtgga
     1561 taagttggca cgcgaaaaaa aatatcgtgt tgaatttaaa aacaataaga tagttctatt
     1621 ggacaaagta gaagatgaaa atcttaaaca aaaaatagaa aattttaaat ttttcgggca
     1681 atatgccgat tttaaagact tgaaaaatta taaaaatgga agaatatcta gcaatgaaaa
     1741 tgttccttat tatgaagcag agtacaaaag gaataatagt gatggaaatg taaaaaaact
     1801 tagagaaaag tacccaatta caaccaagca gtctccaata ttaaaactgc atatagacgg
     1861 cgatattaaa ggtagctcag ttggatataa acagatagaa tacacatttt ctaaggagaa
     1921 agatgatgag acttttatga gtgatttttt gaacttcggt ccatcacaca gtaaatagta
     1981 aataaaacca agggcataaa ctaatttaaa acaagagaga agtgaaaaga tgggatattt
     2041 taaaagagtt gtactttaca taattgttat ggttgtgagt gtttttataa taggttgcaa
     2101 taaatcaagc gatacttcag aaaagccaaa agaagattca aaagaaacac aaatcaaaaa
     2161 gagctttgcg aaaacgttag atatgtatcc aattaagaat ctcgaggact tatatgataa
     2221 agaaggatac cgtgatggcg aatttgaaaa aggtgacaaa gggatgtgga cgatatatac
     2281 agatttcgcc aaaagtaata aaccgggtga actaagtaat gaaggcatgg tcttgtactt
     2341 agatagaaac acacgcacag ctaaggggta ctactttgtc aggacgtttt atcgcaagga
     2401 taaattacct gatagaaaaa attataaagt tgaaatgaaa aataataaaa ttatcttatt
     2461 agacaaggta gaagatccaa atctaaaaaa gagaatagaa aactttaaat ttttcggaca
     2521 atatgcaaat cttaaagaat tgaaaaacta cagcaatggt gatgtctcaa ttaatgagaa
     2581 tgttccaagt tatgacgtga aatataaaat gagtaataaa gatgaaaatg ttaagcaatt
     2641 aagaagtcgt tataatattc ctactgataa atcaccggta ttaaaaatgc atattgatgg
     2701 taatttgaaa ggaagttccg ttggagatag gaagttagaa attgattttt cgaaacgtga
     2761 aaatagccat ttatcagtaa tagattcttt ggattaccag ccagcgaagg tggatgaaga
     2821 tgaaagataa atacaaaata gatccaggga ttattaaaaa taatacagag gaaactacag
     2881 ctatttctaa aatcagctac gaagttgaaa atgcgaattt atatggagct gatagtgaag
     2941 atataacaag acaaatagaa tatttgaaag ccaaaaaaaa attcccttct aatcttgaat
     3001 atgttgatag ttacactgat tctcttaatg gagtaacaac ttctgctttt ttaaataaag
     3061 atacaggcaa agtaactctt gggatgactg ggactaatat acaaggcgaa gcctttaaaa
     3121 agttaaaaga aggtgaattt tcaagacaaa atgttaccaa tgctttggaa acagttaaag
     3181 atggatatgc agatcttaaa atattatatt ctcctgcatc tgatcaaaac tatagatatg
     3241 cgaatacaca agaatttata aataaaataa aaagtaagta tgacattgat tttattactg
     3301 gtcattcact aggtggaaga gatgcggtag ttcttggaat gagtaatggt attccgaaca
     3361 ttgtggttta taatccagct cctatttcta taaatagttt gaatcctaat tccccagatg
     3421 gaaaacgatt gttagagtta tataaaaatt ataaaggtaa tattactagg tttgttgcag
     3481 aaaatgatgc attgacagaa aatctgaaga aatataagca ttatgttttt ttcggtaatg
     3541 ataaagtctt taaaaatggt aaaggtcatg aaatggaagg ctttctgacc gaagaagaac
     3601 aaaaagctat aaaaaaagaa cttaaaaaac tacaaggtta tgcagaagaa aataataagt
     3661 catttgtaaa gaattcaaat aatgctatct ctaaattagc tagtatagaa ttacttagag
     3721 caaatatgat gactacaaat ggaggaggat tatcttcttc gcagcaaaaa gttttagaaa
     3781 gtttaacagc tttaacaatt gcgcagtcat tcagtcaact gatagacgat gaaattaatc
     3841 aaatcaaaaa aatgtataat gaaaagaaaa aggaatttgg aaaaaattgg gaagacgctc
     3901 aaaaagctgg aaaagctgta ggtgaagatt tgagtgtaaa tggagttctc aatgctttag
     3961 atgaaggtca agtgaatgaa agtagtatgg taagagaacc tgaacaaatg atatctgcaa
     4021 aagaaagaca actttcaacg atagggtctt ctgtatcaaa ttatattatg agagttagac
     4081 tcagtattaa tgaaatcgtt gataaagatc aagtgcttgc atcacaaata ggtggattac
     4141 tatgatgttt aatcaaatta ataataaaaa tgaattagaa gaatcatatg aatctgagaa
     4201 aaaacgtata gagaatgaac tgcataattt aaatgaactt aggcatagaa ctcgaaaaga
     4261 aaatgaacgt agttatgatg tttttcaata tttgaagcac gaaatgaatt atagtgaaga
     4321 tgcccaaagg aaaatgacga gaaatataga agcgtatgag caagaaatca atgagataat
     4381 tagaaagcaa gaatggaaat tagaagaata taaagaagac ttaaaaaagt cttatgaaaa
     4441 gcagttagat aaactaagtg actgataatt tggaggagat taaatgactc taatagaacc
     4501 agatatgaac ttaagaatgc cagatataag tactacagta gaaacactta atctcatatc
     4561 taaaatggaa gcgcaaaaag aaaacattcg ttcagttatt gcacctgaac ataagcataa
     4621 atataaagat attgaaaacg gattaaaagg tgaagaaaaa gtattgattg aacaaatggc
     4681 gcagcattgc gaagctttta aagctaattt taaaggtgca gctcaaggag actgggttaa
     4741 aagtgcaatg tctgagatag acagtattaa agatgatttg aaaaaaatta atagctaaac
     4801 tacatatcat actactaaat aaagcgacca atgttcagta tattcacaac tgacactagg
     4861 ccgcttcttt ttaatttatt ttatttccat gtaaagtaac gctctcttaa tcgaaatacc
     4921 acactcacaa ttgtaaaaac acaacttaaa ttactgtccc gcattaaaca tatctacatt
     4981 ccaaacagca acaacaaaat cctcaagtac caaaagctat gaccacttta tcaaaatcaa
     5041 cgcaagttaa cctctcccga actacccgct atagatcatc atcaccttac aaaccataaa
     5101 gcgctgcagt taagtattga atagcatagc caattgaaat gaaaatagca atcgttataa
     5161 tggcactacc aattgtatat aaagtatgtt tcgtagataa accttccgtc ttagaaccaa
     5221 aaacacttag tagaataaat gcaatgccaa tgacatataa aactaaaatg acaaataaca
     5281 tgtttataca cctcgattct ctttagtgag atatatcttt gtctccatta taaagtaaag
     5341 tgctcaataa tttaattaag tatactattt ttttattatc gaagattgcg aaagaggaaa
     5401 cgctcctata taatgtaaaa cggaattatt cctatttaca aataaattga agtgagggta
     5461 tatatatggc taaaattcca gttacggtat taagtggtta tttaggctcg gggaaaacaa
     5521 cattgttaaa tcatatttta caaaatcgtg aaggtcgacg tatcgcggta attgtaaatg
     5581 atatgagtga agttaatatc gataaagatc ttgtcgcaga tggtggggga ctatcgcgta
     5641 cagatgaaaa attagtcgaa ctctctaatg gttgtatctg ttgtacactt agagatgatt
     5701 tattaaaaga agttgagcgt ttagtgaaaa aaggtggcat cgatcaaatt gttattgagt
     5761 caacagggat ttcagagcca gtacctgttg cacaaacttt ctcatatatt gatgatgaac
     5821 ttggcattga tcttacagcg atttgccgtt tagatacaat ggttacagtt gttgatgcta
     5881 accgcttcgt acatgacatc aactcagaag atttattgat ggatcgtgat caaagcgttg
     5941 atgaaacaga tgaacgttcg attgctgatt tattaattga ccaagttgaa ttttgtgatg
     6001 tattgattat taataaaatt gatttaatta gtgaagaaga acttgcaaag ttagaaaaag
     6061 tgttaagcgc attgcaacct attgctaaaa ttattaagac aacaaattct gaagtagatt
     6121 taaaagaagt cttaaatacg cagcgttttg attttgaaaa agcgagcgag tcagcaggat
     6181 ggatcaaaga acttgagtcg ggtggtcatg catcgcatac gcctgaaaca gaagaatatg
     6241 gcatatcatc atttgtatat aaacgtcgtc tacctttcca tgctaaaagg ttcaatgatt
     6301 ggttagaaag catgtcaaat aatgtcgttc gatcaaaagg tatcgtatgg ttagcacaat
     6361 acaatcatgt agcatgttta ttatctcaag cagggtcatc ttgcaatatt cacccagtta
     6421 catattgggt ggctagtatg tctgaagcgc aacaaacaca aatattagca gaacgtcaag
     6481 atgtcgcagc tgaatgggat ccagaatatg gcgatcgtca tacacaattt gtcattattg
     6541 gtacagaatt agatgaagaa aaattaacaa aagaactcga tgcatgctta gtcaatgcgc
     6601 aagaaattga tgcagattgg caacaatttg aagatccata tcaatggcaa attagaccag
     6661 cacgataagt tgaatgaagt ataatcattt ttgaattgtg gcttaattgt ttgcaattta
     6721 tgacaattaa taaaaagtta agatgttctt aggaactttt tgttttcaat ctatctaaag
     6781 tgtgatagtt ttgataaagc agaaatttgc attttatcca tagggctagg acatgtatgt
     6841 gtcttagtcc tttttatatt tgcgttgatg aaaaatgata aaaccatcgt accaactgtt
     6901 aattgaaagt acaattattg tttaacggtc ataaaaatga gtgaagtaat tgcagctatc
     6961 aatgtactat tactgcttac agtcacacga gtgtttcaga ctaaacgtgc aacgaaattc
     7021 tgcacgcggc caatatatct tgcgccagat caagaagatc attaagattt cgcacgccaa
     7081 atcatgaatg tcttagactt cgagtatcaa acataaaaca cgccacaaaa atataacgcg
     7141 tcagactaca aagatcacga atattcctta cgtaccacca agaacatcct atacaaaaaa
     7201 taccaagacc atttatttgc aacaaacaaa caagactcac ctcacatcaa taaatcaaca
     7261 caaagcaaag ccaccatccc tattggtata gtggcctgag aattttaagt attcaattcg
     7321 cttaaattat tttgcgaaaa tgtcgataat tgctttgatg attttaatga tagtacctac
     7381 aatagccatc gttttgtcct cctgtatgtt gatttaacat agtgatgagt tgttgattgt
     7441 taatgtttga gattagttgt tacctaaaaa tttaccaagt aaatctttaa agaatttgaa
     7501 taattttgct acgaattcca tgtgaatggc ccccttcaaa taagatgttc atatctatga
     7561 gatttttata acttacttac cagtgaattt ctcaattaat cctttaatga atttaatgat
     7621 tcctgcaatg atacccatgt gaaagacctc ctttgtttgt tatgaaatct tatttaccag
     7681 tgaattgttc gattaagctt ttgataactt taatgatgcc agcgatgata cccattaaga
     7741 ttacctcctt tgcttatgag ttaacttcat tgtacatagt tatcttgtgc gtaattgatt
     7801 tttttcctaa cttgaattgt tatgatcata actgcgaatt ttttatttta aaaggacggg
     7861 aaatactgcc taactgtaca ggtgcgttta caaaatcttt acaatcattg gtttaataga
     7921 ggttaagctc atgaatttga catgaagcag aaatttaatt atattttatg ttataagttt
     7981 aaaacgaata acacgttagg tctcgtctag gcaaagcata tttatgaaga tgaattgaac
     8041 agacagaagt aacattttat atacatatgt gcatgggagc acgcttaaag agaacactgc
     8101 aacaaggtat atcaacgtac cgtgatgcag tgtctagtta aactgaaacc tgcaatagta
     8161 aattttctgc cattatgtac agaatctact attgtaggtt tttattttta tacataagca
     8221 attatgcgag agattagaaa taaggaggtt atgcagtgtt aagttttcaa ttgctatttt
     8281 cactgtttgt tattgcgctt atcattgcat tgataagtgg cttgttgttt ttagcaccag
     8341 ttatgccaat gagatatatt aaattacatt tatacatact agtcatgcca gtattatttg
     8401 cagtcattgg ctttttcggt attcatggtc aacatgtctt aggtccattt aaaatagatc
     8461 gtttatcttg gttattagct ggctttgtaa tggcgcttgg ttttattatt caaaagtttt
     8521 caatgcgata tttactaggt gatcatcatt atagacatta ctttccattg ttcactgcga
     8581 ttacgtcgtt tgcatcttta gcatggatgt ctgaagactt aagactgatg gcactctgct
     8641 ggggtatgac attattatgt ttaacactgc tgatgaacgt taatcgtttt tggaaagtgc
     8701 cacgtgagtc tgcgaaatta tcaagcatga catttttatg tggttggctt gcattcgttg
     8761 gagcaattgt aactatttat attgcgactg gtgagtggcg ggtaccacaa catatagtac
     8821 atccgacatg gtcattgttg acgaatgtac tacttgtatt agctgtcatg ataccggcag
     8881 cacaatttcc ttttcatcga tggttgattg aatctgtaac ggcaccaacg ccagtatcgg
     8941 caattatgca tgcaggaatt gtgaatgcag gtggtgttat tctaactcgt tttgcgccga
     9001 tatttgataa tggatttgcg ttatcattat tacttatcct ttctagtatt tctgtattgt
     9061 taggatcggg tattagcttg gttcaagttg actacaaaag acagttagtg ggctctacga
     9121 tgagtcaaat gggctttatg ttagttcaat gtgcattggg tgtatattca gcagcgatta
     9181 ttcatttaat attgcacggt atttttaaag caacattatt tttacaatca ggttctatcg
     9241 tgaagcgatt caatattcca aaacaagcat ctgctaaaga cgcttatggc tggattgtca
     9301 tgggacgtgt attagctatt atcgtggcat ttctattttg gatgagtagt gacagaagtg
     9361 catatgaagt gttaagtgca ctcattctag catggtcatt acttgtatct tggaatcaga
     9421 tggtagcctt tagtaaaggg cgcatggcac gtctggttgg catgattttg attgcaattg
     9481 tgacatttat ctacgtcatc acacacaatt atttttacga tgtattacaa aatataacaa
     9541 cacatgcgac aacaccgcct acagtgagtg tcatcattag tgttgtcatt ttaatctttg
     9601 gcagtttatt aagtatttgg gtggcgcgtc atcgatactc taagggtttt gcggtattgt
     9661 acgtgtggtt agttaatcta ggtgaagcac gctcgaaagc gatagaaagt catccgaatt
     9721 atttgaagaa gtatttatag gaggtgaaag gtatgacaac acagttaaat atcaattcag
     9781 tcattgaaaa tgcgaaacgt gttattacac cattatcacc gatttcgatt tttgcagcac
     9841 gtaacccctg ggaaggatta gaagcggata cgtttgaaga tgtcgcaaaa tggttacgtg
     9901 acgttcgcga tgtggatatt ttcccaaata aagcattaat agaaagcgct gtggcacgtg
     9961 gtgaattaga tgaaagtgtc tttaatcaac ttgttactga tatgttactt gaacatcact
    10021 acaatatccc gcaacactac atcaatcttt atattgataa cattaaaaca ttaaaagacg
    10081 tacctgcatc atatatgaat cattcaaatg ttgatgttgt tgctgatcta ctattagaaa
    10141 aatcaaaacg tgatatggct gaatcatatc atcactatga tgtacgtccg atgagtgatg
    10201 caataataga tgaacaaggt gagccactta gcgaacaagt gaatcgtcaa atgattaaat
    10261 ggacgaaact ttatatcgat caatttctat cgagttggac aatgccgaag cgtgagcaaa
    10321 gtttttacca tgcatggttg catttagcgc aacatgacca tagttttact aaagcacagc
    10381 gccaagtgat taaaggctta cccaatgatc ctgaaatgac gatagagtca gtattaactt
    10441 atttttcaat agatcaggaa gactaccaag cttatgttga aggacatctt ttggcgttac
    10501 cgggttgggc aggtatgttg tattaccgtt cacaacagca tcactttgaa caacatttgt
    10561 taacggatta tttggcaatt cggttagttg tcgaacaatt gctagttggt gatgagttta
    10621 agtcagtcac taaagattgt gaaagtagat cggaaaattg gtttaagcaa actgttgcat
    10681 cattgtgtta ctacagtgat atgcctagcg atgtattact acaacatgac gtcaatgaaa
    10741 ttcaaacgtt tattcatttt gcagcaacta tgaataaaaa tgtatttaaa aatttatggc
    10801 taattgcctg ggaaatgaca tacgaatctc agttaaaaca aaaaattaaa gcaggtcatg
    10861 aaagtgtggc gggcgcatta gatgtaaacc aagtaaatgt ctcagaaaat gataacgcta
    10921 atcagccaca ttcagtattg ttaaatgaca cacaagcagt tgatgaaaat aatagcgagc
    10981 taaatcaggt gggcacatca acgaaagcgc aaattgcatt ttgtatagat gttcgttcag
    11041 aaccatttcg tagacatatc gaagcagcag ggccctttga aacgattggt attgcaggtt
    11101 tctttggatt acctattcaa aaagatgccg tagacgaaca attcaaacat gattcattac
    11161 ctgtcatggt accgccggca tatcgcatta aagaatttgc agaccgctac gatatgaatg
    11221 tttatcgaca acagcaacag acaatgtcat cgatgtttta cacatttaaa ttgatgaaaa
    11281 ataatgttat gcctagtctg ttattgcctg aattaagtgg gccattttta agcttgagta
    11341 ccattgtcaa ttcgattatg cctagaaaaa gtcgcgcgtc tttacaaaaa ataaaacaaa
    11401 aatggttgaa aaagcctgaa acaaagttaa cgattgatcg tgagtttgac cgaacatcag
    11461 acttacctgt tggatttact gagcaagagc aaattgattt cgcgttacaa gcgttgaaat
    11521 tgatggattt aaccgaagca tttgcgccgt tcgttgtgtt agcaggtcat gctagtcatt
    11581 ctcacaataa tccacatcat gcatcacttg aatgtggggc ttgtggtggc gcatcaagcg
    11641 gttttaatgc taagttatta gcgatgatat gtaatcgtcc aaatgtcaga caaggattaa
    11701 aacaatcagg tgtgtatatt ccagagacaa ctgtttttgc ggcagcagaa catcatacgt
    11761 ctactgatac gttggcatgg gtatatgtgc cagacacatt atcagcttta gctctagatg
    11821 catatgaatc attgaatgac gcgatgccga tgatttctga acaagcgaat cgcgaacgtt
    11881 tggacaaact gccaacgatt ggtcgtgtga atcatccagt ggaagaagca cagcggtttg
    11941 caagtgattg gagtgaggta cgtccagaat ggggcttggc taaaaatgca tcatttataa
    12001 ttggacgacg ccaattaaca aaaggcattg atttagaagg gcggacattt ttacacaatt
    12061 atgattggcg taaagataaa gatggcaaat tgttaaatac catcatttct ggtccggcgc
    12121 ttgtggcaca atggattaat ttacaatatt atgcgtcgac agttgcgccg catttttacg
    12181 gaagtgggaa taaagcgaca caaaccgtca cgtcaggtgt tggtgtcatg caaggtaatg
    12241 cgagtgatct gatgtatggc ttatcatggc aatctgttat ggctgctgat cggacgatgt
    12301 atcattcgcc aattcgttta cttgtcgtta ttcaggcacc cgactatgtt gtagctagac
    12361 tattcgcgaa taatgagcat ttcgctagga aggtgtctaa tcattggctg cgtttaatga
    12421 gcgttaatga ggaagggcgt tttaaaagtt ggatttaacg atgatatgtt gcgatgctaa
    12481 gtaattaata tatcatcata tctacaatgt tctagcttct taaaatatac taaaaatgat
    12541 aaacttaatg taatagttga tgtcgtgata acgatacaaa gatgaagtaa tcaattttaa
    12601 tattattgga gttagtgata tgaaaagaac gaaaggtgaa atcgaagctg aaatcagtaa
    12661 agccattacg caatgggaaa aagatttcct tggcagaggt tccttgtcag ttaaatcaga
    12721 cattttaaga gatatggtga ttattagttt acaaggtatc ttaacgccag cagaatatcg
    12781 tgtgtgtagt acgaatgaag gattactaaa tattaaacga acacgttctg aattagttga
    12841 atccggtgag caagatttga atgatatcat tcttaaaatt acaggtatca aagtgatgag
    12901 cttccacagt gatttaagta cagttacagg tgaacgtatt atcgtattca aacttgagga
    12961 taatttggaa aagcatattt aagaaggtta ggtatgcttc tgctggctta ttaaagctat
    13021 agaagtatgc ttggttaaac agcgcaacgc catatatttg ggtgtgctgt ttttctatta
    13081 catgatgtgt ttattacgtg gatgaatgcg tgggtctgaa tatagcccgt gaaaggcgta
    13141 tgtcataaaa tttgcgaatg gtgacaagtc aacgcaagca tatttgttgc gagacatgcc
    13201 cactatcctt ttacataaag agtatatgtg tgacgtaggc atataatcga taaagtatac
    13261 ctaaaaaatt aggtataatg accattgttg caataagttt ttcggccttg aaaccgataa
    13321 cgcatagtat aataggaata aaatataata caaatatcca gtaaatcaaa tttgaaaacg
    13381 atggatgaat aggggtaaag aatctaacta atgtaatgat taacgctata tagacaaaaa
    13441 taactctatt gatacgcctt accccctctg tataataaat atagatcgta ctaaattgaa
    13501 aatagcaaaa tattgttatt tcaattatac aatgttttat ttgcaatata cataacaata
    13561 tgaactttat aattttgata ttgtcggtaa agtgaaattt gtggcacatt gatattatgg
    13621 ataacatttg taaaatgaaa atgatgatac gaggagattg taagatgata gataaaaaat
    13681 taacatcacc gaaaatgaca gtgcctttat ttttaatcgc gctgattgta tttataggta
    13741 tgttttacgg tgtagtgaca aatcaagaat ggcttaaaaa tatagatatg ggatcattaa
    13801 catggtttac agattatttc ggtgagccac aacgtcagta tgttaacaat ttgtttaatt
    13861 attatatgac gtttagtgcg gaaattggag atgtcaaagg tgtcgtgttg atttcaatta
    13921 tcgtcacaat catactgttt attaaacaga ggcatttagc ggtttggttt gtgacatatt
    13981 tggtttcagg tgtcatcatg aacaaattaa ttaaagatac tgttttacgt ccaagaccat
    14041 ataatcattt agccgttgat acaggctttt catttccaag tggacattcc aacgccagca
    14101 cattattata tttcgcctta atgatcatta ttatttcagt tgctgctaag acaataacaa
    14161 aagtgttgag tgcgctagtt atgggaatat tatggcttag catattattt tgtcgccttt
    14221 attttcatgc acattacttt tcagatgtca ttggcggcac gtcactagca atcatttggg
    14281 tagcgttatt cttaatggta tacccatact ttattaatca tcgacgacaa cgcgtttagc
    14341 aaacatcttt agacatagtg gaggtatata gaaaaatgag aattaaaaca ccgagtccat
    14401 cttatttaaa aggcacaaat ggacatgcga tattattatt acattcattt acaggtacaa
    14461 atcgggatgt gaagcatctt gcagctgagt taaatgacca aggatttagt tgttatgcac
    14521 cgaattatcc aggtcatggt ttactgttga aagatttcat gacatataat gtagatgatt
    14581 ggtgggaaga agttgagaaa gcttaccaat ttttagtcaa tgaaggttat gaatctatca
    14641 gtgcaacggg tgtgtcttta ggtggattaa tgacattaaa attggcgcag cactacccgt
    14701 tgaaacgtat cgctgtcatg tcagcaccaa aggaaaaaag tgatgatggc ttaatagaac
    14761 atttagttta ttatagtcaa cgcatgtcga atattttaaa tttagatcag caagcatcga
    14821 gtgcgcaatt agcagcaatt gatgattatg aaggtgaaat tacgaagttt cagcatttta
    14881 ttgatgatat catgacaaat ttaaatgtta ttaaaattcc agctaatata ttatttggtg
    14941 gtaaagatga gccatcctat gaaacaagtg cacattttat ttatgaacat ttaggatcag
    15001 tagacaaaga attaaatggt ctgaaggatt cgcatcattt aatgacacat ggagaaggca
    15061 gagatatttt agaagaaaat gttattcgct ttttcaatgc tttaacataa ttgaagattt
    15121 taaatattaa cacttgagtg acgatgttat tatatttgtc atttgagtgt tttttgtggc
    15181 gtgatagtat agggaactta gacaaagtta atttgtagtg tgatgtcgtg gaaatattat
    15241 gggcattgta cctgaagttg ttatgtggcg agatattggc gcacgtatac agcgttgaac
    15301 cagaaatttt aatgtagcgt aatgacaaca cattacaaga gttgactata ttatgataaa
    15361 tacaggcgct accgtattaa tgaaacaatt ttgatgcagg tgtattgcat gttatttaca
    15421 tacatgttag agtgttactt aaacacgtag acgctttaac gctttaaagg agatgtgaat
    15481 gatgaaaaga caacaatcac aatggaagtc atcaactgga tttattttag ctagtgcggg
    15541 ttctgcaatc ggtcttggtg ccatgtggaa attcccatat atggcaggga tttatggcgg
    15601 cggtgccttt ctagctatgt tcttaatatt caccattttt gttgggttgc cattactcat
    15661 tatggaattc actgttggga aaatgggacg gacatataca acacaaatat atagtaaatt
    15721 aactggtaaa aaatggctca atatcattgg ctggaacggt aatttggccg tgtttatttt
    15781 atttggcttc tatagtgtta tcggtggttg gattgtcatt tacatcggac aagtattatg
    15841 gcaattagtt atatttcaac gcatcaatca tctccaagaa atgaattttg aagcggtaat
    15901 atcaaatcct tggttaactg ttctagggca aggtatattc atattcgcta cgatgattat
    15961 tgtcatgtta ggtgttgaaa aaggattaga aaaggcatcg aaagttatga tgccattgct
    16021 gtttgtcttt ttaatcatca ttgtgattaa gtctttaaca ttagatggtg ccttagaagg
    16081 tgtgaaattt attttacaac caagagtatc agagattact gctgatggca tcttgtttgc
    16141 gctaggtcaa tcattcttta cgttatcatt aggaactaca ggtatgatta cttatgcgag
    16201 ttatgcctct aaagacatga cgattaagtc atcagctatt tctatcgttg ttatgaatat
    16261 ctttgtatct gtattggcag gcctagctat atttccggcg ttacatagtt ttggctatga
    16321 accacaagaa gggcctggat tattatttaa agtactgcca atggtcttta gtcaaatgca
    16381 tctaggcaca ttattctatt tgggattctt agtgctgttc ttatttgcgg ctttaacgtc
    16441 atctatttct ttattagaat taaatgtttc taacttcacg aagaatgaca atacaaaacg
    16501 taaaaaagtc gcagtgatcg gtagtatttt agtatttatc attagtattc cagcaacctt
    16561 atcttttggt atcttaaaag atgtaagatt cggtgcggga acgatttttg ataatatgga
    16621 tttcatcgtt tcgaatgtat tgatgccatt aggcgcatta ggtactacgc ttgtcatagg
    16681 acaattatta gataaaaaat tattacaaca atattttggt aaagatcgat ttagattatt
    16741 caatggttgg tattacttaa ttaagtatgc gatgcctgtc gttattattt tagtctttat
    16801 cgtgcaatta tttagttaat atatagaagc atctgacaca caagtatttg tgttgctcag
    16861 gtgttttttt gtttggaatt gaaaagttag tactggttct gaattaatcg aaacttagat
    16921 aattgcaaaa taacaaaatg tcgtttataa ttcttatctg aaaagtaaga attaaaattg
    16981 agacatgaag aaaggtttca ttcatttgat cggcatgtca gatgagggtg catctatgat
    17041 tacttatgat ttaattggca atacaccatt agtactgtta gaacattata gtgatgataa
    17101 agttaaaatt tatgccaagc ttgaacaatg gaatcctgga ggcagtgtta aagacagact
    17161 cgggaaatat ttagtagaga aggcaattca agaagggcgt gtgcgtgcag gtcaaactat
    17221 tgttgaagcg actgctggta atacaggcat agggttagct attgcagcga atagacatca
    17281 tttgaaatgt aagatctttg cgccgtatgg tttttcagaa gaaaagatta atattatgat
    17341 agcgcttggt gcagatgttt caaggacgag tcagtctgaa ggtatgcatg gggcacaatt
    17401 agctgcacgt tcctatgctg aaaaatatgg tgtcgtttat atgaatcaat ttgaatccga
    17461 acataatccg gatacatatt ttcatacatt gggacccgaa ttgacttcag cattacagca
    17521 aattgattat tttgtggctg gtattggctc tggcggtaca tttacaggta ccgcacgtta
    17581 tttaaagcaa catcacgtgc aatgttatgc cgttgagcca gaagggtccg tgttaaatgg
    17641 agggccagct catgcacatg acactgaagg tatcggttct gagaaatggc cgatattttt
    17701 agagagacgt cttgtagatg ggatatttac gattaaagat caagatgcct ttcgaaatgt
    17761 caaaagtttg gctataaatg aagggttgtt agtaggcagt tcttcaggtg cagcattaca
    17821 aggtgcattg aatttaaaag cgcaattatc tgaaggtacg attgttgtcg tatttccaga
    17881 tggtagcgat cgatatatgt ctaagcaaat atttaattat gaggagaatg attatgaata
    17941 agaaaactaa attaattcac ggtggacaca caacggacga ctatacaggt gcagttacta
    18001 caccaattta tcaaacaagt acatatttac aagatgatat tggtgattta cgtcaaggat
    18061 atgaatattc tcgtactgcg aatccaacaa gaagttctgt agaaagcgtt attgcggcat
    18121 tagaaaatgg caaacatggc tttgcgttta gttcaggtgt tgcagctatc agtgcagttg
    18181 ttatgctgtt ggacaaaggt gatcatatta ttttaaattc agatgtatac ggcggtactt
    18241 atcgcgcatt gacaaaagta tttacacgat ttggcattga agtggatttt gtagatacaa
    18301 cgcatacaga ttcaattgta caagcgatac gcccaacaac aaagatgttg tttattgaaa
    18361 caccttctaa tccattatta cgtgttactg acattaaaaa gtctgctgaa attgcgaaag
    18421 aacacggttt gatttcagtc gttgataaca catttatgac accttattat cagaatccat
    18481 tagatttagg catcgatatt gtcttgcatt ctgcaacgaa atatttaggt ggacatagtg
    18541 atgtcgttgc tggtttagtt gcaacatcgg atgacaagct tgcagaacgt ttagcattta
    18601 tttcaaattc aacaggtggc attttaggac ctcaagatag ctatttactt gtgaggggta
    18661 ttaaaacatt aggtttacgt atggaacaaa ttaatcgcag tgttattgaa attattaaaa
    18721 tgttacaagc acatccagct gtgcaacaag tgttccatcc aagtattgaa agtcatttaa
    18781 atcatgatgt ccatatggct caagcggatg gccatacagg tgtgattgca tttgaagtga
    18841 aaaatacaga aagtgccaaa caattgatta aagcaacatc gtattacaca ttagctgaaa
    18901 gtttaggtgc agtggaaagt ttaatttcag tacctgcatt gatgacacat gcatccattc
    18961 cagcagatat tcgagctaaa gaaggtatta cagacggact tgtaagaatt tctgtaggta
    19021 ttgaagatac tgaagattta gtcgatgatt taaaacaagc actagatacg ctataaataa
    19081 tagcagcact agcatatatt ttaagatcaa cttaaattga ttttgaaaat gctagtgctt
    19141 ttttgtgcga tgttaatcat tcgtgattga gttatggagg tttggtggac atttgtatgg
    19201 atttaatttg ataaatgtca tagtagtctc acaattcatc attgtcacat gaattaatta
    19261 tttttttaat tttttagaaa attcggcaaa ttgtattgac gatatatgca attttacata
    19321 taatgctctt attcctagtg gattaataag aatttgtagg aggggcgatg atgattgagt
    19381 ttcgacaagt tagtaaatca tttcataaga aaaagcaaac aatagatgct ttgaaggacg
    19441 tatcatttac ggtcaatcgc aatgatattt ttggtgtgat tggatatagt ggtgcaggaa
    19501 aaagtacgtt ggtaagactc gtgaatcatc ttgaagctgc ctcgaatgga caagtgattg
    19561 tagatggaca tgatattacg aattatagcg aaaaaggcat gcgagaaatt aagaaagata
    19621 tcggtatgat atttcagcat ttcaatttat taaattcagc tacggtattt aaaaatgtag
    19681 caatgccact cattttaagt aagaaaagca aaacagaaat taagcaacga gtaacggaaa
    19741 tgcttgaatt tgtaggattg agtgataaaa aagaccaatt tcctgatgaa ttatctggtg
    19801 ggcagaagca aagggtggct attgcaagag cgcttgttac taatccgaaa atactcctat
    19861 gcgatgaagc aacaagcgca ttggatccag caacgactgc ttcgatattg acgttattaa
    19921 agaatgtcaa tcaaaccttt ggcattacaa ttatgatgat tacacatgaa atgcgcgtta
    19981 ttaaagacat ttgtaatcgt gttgctgtaa tggaaaaggg gcaagtggtt gaaacaggaa
    20041 ctgttaaaga ggtgtttagt catcctaaaa cgacgattgc tcaaaatttt gtgtctacag
    20101 ttatacagac tgagccaagt acatcattga ttcgtcgatt gaatgacgaa caagttggcg
    20161 gttttaaaga ttataaaatc ttcgtcgagg aaactcaggt gacacaaccg attataaatg
    20221 acttgattca aatttgtggc agagaggtta aaattttatt ttcatctatg tcagaaatac
    20281 aaggtaacac cgtatgttat atgtggcttc gatttaatat agatcaacaa tttgatgaca
    20341 cggcaataaa tcaatatttc aaagagaaaa atattcaatt tgaggaggtg cattaacata
    20401 tgtttggttc tgatttagac agtgcacagt tattacaagc attgtacgaa acgttatata
    20461 tggtatctat tgctttattt ttaggagcag tgattggtat tccattaggt gtcttattgg
    20521 taattactcg aaaacaaggc atatggccca atatagtgat acatcaagtt ttaaatcctt
    20581 taatcaatat tttaaggtca ctaccattta ttattttgtt aattgcgatt gtgccattca
    20641 caaaattagt agtaggtact tcaattggta cgactgctgc catcgtgcct ttaactgtat
    20701 atgtggcacc ttacattgca agacttgttg aaaactcatt attggaagta gacgagggga
    20761 ttattgaagc ggcgaaagcg atgggcgctt caccactaca aatcattaga tattttttaa
    20821 ttcctgaagc gttaggttca ttagtattag caattaccac tgcgattatt ggacttattg
    20881 gaagtacggc gatggcagga gctgttggcg gtggtggtat aggagactta gctttagtgt
    20941 atggttatca aagatttgat acgacggtca ttattattac cgttattgta ttagtcatta
    21001 ttgtccaagt gattcaaacg ctagggaatg tcctagctag attcatacgt agacattaat
    21061 gatatatagt gaagattttg aaaggaattg atagaatgaa aagattgatt gggttagtta
    21121 tcgtagcact tgtattatta gcagcgtgtg gtagtaacaa tgataaaaaa gtaacaattg
    21181 gtgtcgcatc aaatgacact aaggcttggg agaaggttaa agaattagct aaaaaagatg
    21241 atattgatgt ggagattaag cacttctccg attacaattt accgaataaa gcattaaacg
    21301 atggtgatat tgatatgaat gcattccaac attttgcatt tttagatcag tataagaagg
    21361 cacataaagg aacaaagatt tcagcattga gtacaacagt tttagcaccg ttgggcattt
    21421 actcagataa aattaaagat gtcaaaaagg ttaaagatgg tgctaaagtt gtcattccaa
    21481 atgatgtatc aaaccaagca cgtgcactta aactattaga agcagctggt ttaataaaac
    21541 tgaaaaaaga tttcggatta gcaggtacag tgaaagatat aacgtcaaat ccaaaacatt
    21601 taaaaattac tgcagtagat gcacaacaaa ctgcgcgtgc tttatctgat gtcgatattg
    21661 cagttattaa taatggtgta gcaactaaag cgggtaaaga tcctaaaaat gatccgatat
    21721 ttttagaaaa atcaaattca gatgctgtaa agccatatat taatattgtt gcagttaatg
    21781 acaaagactt ggataacaaa acatatgcta aaatcgtaga attgtatcat tcaaaagaag
    21841 ctcaaaaagc gttgcaggaa gatgtcaaag atggagagaa acctgttaat ttatctaaag
    21901 atgagattaa ggcaatagaa acgtcattag caaaataaat tatattgcgt cctacaagca
    21961 aagttcatgc ttacgtttgt agggcgttat tgtttgacaa taaaattatt tccaatagag
    22021 aaagggaatg taatcatttt atagtgaaat attatgaaat tgtaataatt tagatattgt
    22081 aaaatctaat aagt
//
LOCUS       DAOBBH010000025        22062 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.025, whole genome shotgun sequence.
ACCESSION   DAOBBH010000025 DAOBBH010000000
VERSION     DAOBBH010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22062
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.025"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(521..1903)
                     /locus_tag="P6L01_000698"
     CDS             complement(521..1903)
                     /locus_tag="P6L01_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDP3029786.1"
                     /translation="MKNTLYHQLYEKLKKQIIEGQFKEGDKFYSKRQLSKHLSISQTT
                     VEHAYQLLLDEGYIYSRPRSGYFVSEIESLTILNNQPIPSLFDDDSYKPKASDEAYDY
                     AFNLDEIDTKHFPIELFRKYSKDLYDTNHLNQLRRGHFQGELHLRFQLAFYLFTNRGV
                     ICDPNQIIIGSSTEQLVNQLVDLLYTSTFIIEKPSYPPIKNILDKKQVEYEQIEVEDN
                     GINVDEVIKSQKNIVYITPSHQFPTGYVMDLKKRTQLIQWAQEKEERFIIEDDYDSEF
                     RYFGKPIPAIQGLYSRGEKVIYISTFSKSIFPSCRVAYMVLPYSIMKKYHSQNHIEGN
                     TVPVHMQNLIATFISSGGFERHLNKMRRIYRRKLTYILKRLKPYKEQLDIQGAETGMH
                     FTITVKNGLTLQECLDRANKVKLKLQVYNFDDDQDYKKTPKFILGFGGIDDDALKPHV
                     DALIKSLIIK"
     gene            2007..2894
                     /gene="pdxS"
                     /locus_tag="P6L01_000699"
     CDS             2007..2894
                     /gene="pdxS"
                     /locus_tag="P6L01_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HDP3029787.1"
                     /translation="MSKIIGSDRVKRGMAEMQKGGVIMDVVNAEQARIAEEAGAVAVM
                     ALERVPSDIRAAGGVARMANPKIVEEVMNAVSIPVMAKARIGHITEARVLEAMGVDYI
                     DESEVLTPADEEYHLRKDQFTVPFVCGCRNLGEAARRIGEGAAMLRTKGEPGTGNIVE
                     AVRHMRQVNSEVSRLTVMNDDEIMTFAKDIGAPYEILKQIKDNGRLPVVNFAAGGVAT
                     PQDAALMMELGADGVFVGSGIFKSEDPEKFAKAIVQATTHYQDYELIGRLASELGTAM
                     KGLDINQLSLEERMQERGW"
     gene            2898..3458
                     /gene="pdxT"
                     /locus_tag="P6L01_000700"
     CDS             2898..3458
                     /gene="pdxT"
                     /locus_tag="P6L01_000700"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HDP3029788.1"
                     /translation="MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILP
                     GGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIVLAQDIVGEEGYLNKLNITVQRN
                     SFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYL
                     GVSFHPELTDDYRVTDYFINHIVKKA"
     gene            complement(3666..4880)
                     /locus_tag="P6L01_000701"
     CDS             complement(3666..4880)
                     /locus_tag="P6L01_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDP3029789.1"
                     /translation="MHILIGIIGIIFFLALAFLFSSDRKNIRWKYVGILLAIQLIFAF
                     ILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFI
                     SAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLP
                     YIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKV
                     NEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSNI
                     FSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALG
                     KLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLV
                     SFISAAIAGFFI"
     gene            5038..5499
                     /locus_tag="P6L01_000702"
     CDS             5038..5499
                     /locus_tag="P6L01_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDP3029790.1"
                     /translation="MHNMSDIIEQYIKRLFEESNEDVVEIQRANIAQRFDCVPSQLNY
                     VIKTRFTNEHGYEIESKRGGGGYIRITKIENKDATGYINHLLQLIGPSISQQQAYYII
                     DGLLDKMLINEREAKMIQAVIDRETLSMDMVSRDIIRANILKRLLPVINYY"
     gene            5518..6084
                     /locus_tag="P6L01_000703"
     CDS             5518..6084
                     /locus_tag="P6L01_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HDP3029791.1"
                     /translation="MLCENCQLNEAELKVKVTSKNKTEEKMVCQTCAEGHHPWNQANE
                     QPEYQEHQDNFEEAFVVKQILQHLATKHGINFQEVAFKEEKRCPSCHMTLKDIAHVGK
                     FGCANCYATFKDDIIDIVRRVQGGQFEHVGKTPHSSHKKIALKRKIEEKNEYLKKLIE
                     IQDFEEAAIVRDEIKALKAESEVQHDDA"
     gene            6074..7081
                     /locus_tag="P6L01_000704"
     CDS             6074..7081
                     /locus_tag="P6L01_000704"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HDP3029792.1"
                     /translation="MTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYAT
                     ENDGFRVINEVQDALPNFELMRLDQMDQQSKMKMVAKHLISPELIKQPAAAVLVNDDE
                     SLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDRSLDISYDEQLGYLTTCPTN
                     IGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLT
                     LGKSELEIIETLTEVVNQIIHDEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITME
                     EASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHI
                     K"
     gene            7095..9551
                     /locus_tag="P6L01_000705"
     CDS             7095..9551
                     /locus_tag="P6L01_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDP3029793.1"
                     /translation="MLFGRLTERAQRVLAHAQEEAIRLNHSNIGTEHLLLGLMKEPEG
                     IAAKVLESFNITEDKVIEEVEKLIGHGQDHVGTLHYTPRAKKVIELSMDEARKLHHNF
                     VGTEHILLGLIRENEGVAARVFANLDLNITKARAQVVKALGNPEMSNKNAQASKSNNT
                     PTLDSLARDLTVIAKDGTLDPVIGRDKEITRVIEVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQAIVNNEVPETLKDKRVMSLDMGTVVAGTKYRGEFEERLKKVMEEIQQAGNVIL
                     FIDELHTLVGAGGAEGAIDASNILKPALARGELQCIGATTLDEYRKNIEKDAALERRF
                     QPVQVDEPSVVDTVAILKGLRDRYEAHHRINISDEAIEAAVKLSNRYVSDRFLPDKAI
                     DLIDEASSKVRLKSHTTPNNLKEIEQEIEKVKNEKDAAVHAQEFENAANLRDKQTKLE
                     KQYEEAKNEWKNAQNGMSTSLSEEDIAEVIAGWTGIPLTKINETESEKLLSLEDTLHE
                     RVIGQKDAVNSISKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESMFGDD
                     DAMIRVDMSEFMEKHAVSRLVGAPPGYVGHDDGGQLTEKVRRKPYSVILFDEIEKAHP
                     DVFNILLQVLDDGHLTDTKGRTVDFRNTIIIMTSNVGAQELQDQRFAGFGGSSDGQDY
                     ETIRKTMLKELKNSFRPEFLNRVDDIIVFHKLTKEELKEIVTMMVNKLTNRLSEQNIN
                     IIVTDKAKDKIAEEGYDPEYGARPLIRAIQKTIEDNLSELILDGNQIEGKKVTVDHDG
                     KEFKYDIAEQTSETKTPSQA"
     gene            10036..11400
                     /gene="radA"
                     /locus_tag="P6L01_000706"
     CDS             10036..11400
                     /gene="radA"
                     /locus_tag="P6L01_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDP3029794.1"
                     /translation="MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPK
                     HGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRVLGGGIVSGSLVLIGGDPGIGKS
                     TLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
                     EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNITTFIVGHVTKEG
                     QIAGPRLLEHMVDTVLYFEGDEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNP
                     SEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPRRMATGIDHNRLSLL
                     MAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGE
                     VGLTGEVRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEALSF
                     ALHS"
     gene            11425..12498
                     /locus_tag="P6L01_000707"
     CDS             11425..12498
                     /locus_tag="P6L01_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDP3029795.1"
                     /translation="MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYV
                     DGIIGSIFMFLIFGVFIRRVTNAIKGLEHFIMRRSAVEILFATIGLIIGLLISVMVSF
                     ILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATP
                     KIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDIL
                     NELYDLDYPTKVIHPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNV
                     NDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEV
                     VSLLQTSSGRIVFAKKIEDTVSL"
     gene            13055..14509
                     /locus_tag="P6L01_000708"
     CDS             13055..14509
                     /locus_tag="P6L01_000708"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDP3029796.1"
                     /translation="MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIE
                     DTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAE
                     DKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
                     QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDH
                     ISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEA
                     LFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDT
                     ETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMP
                     ALGEEEQQVINGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMP
                     IRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK"
     gene            14932..15579
                     /gene="cysE"
                     /locus_tag="P6L01_000709"
     CDS             14932..15579
                     /gene="cysE"
                     /locus_tag="P6L01_000709"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDP3029797.1"
                     /translation="MILLKRMRDDIKMVFEQDPAARSTLEVITTYAGLHAVWSHLIAH
                     KLYNQKKYVAARAISQISRFFTGIEIHPGAKIGKRLFIDHGMGVVIGETCTIGDNVTI
                     YQGVTLGGTGKERGKRHPDIGDNVLIAAGAKVLGNIKINSNVNIGANSVVLQSVPSYS
                     TVVGIPGHIVKQDGVRVGKTFDHRHLPDPIYEQIKHLERQLEKTRNGEIQDDYII"
     gene            15563..16963
                     /locus_tag="P6L01_000710"
     CDS             15563..16963
                     /locus_tag="P6L01_000710"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDP3029798.1"
                     /translation="MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPA
                     INYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQSVPEIAEKYIAAFHEDVGA
                     LNVRKATSNPRVMDHMDDIIQFIKDLVDQGYAYESGGDVYFRTRKFEGYGKLSHQSID
                     DLKVGARIDAGEHKEDALDFTLWKKAKPGEISWNSPFGEGRPGWHIECSVMAFHELGP
                     TIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILV
                     HDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATN
                     IENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDK
                     FKAVYQIFSDVLGVPLKSKNADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQ
                     NIILEDTPQGVRFKRG"
     gene            16956..17360
                     /locus_tag="P6L01_000711"
     CDS             16956..17360
                     /locus_tag="P6L01_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDP3029799.1"
                     /translation="MDNQQDNHIKLLNPLTLAYMGDAVLDQYVRTYIVLKLKSKPNKL
                     HQMSKKYVSAKSQAQTLEYLMEQEWFTDEEMDILKRGRNAKSHTKAKNTDVQTYRKSS
                     AIEAVIGFLYLEKREERLEALLNKIITIVNER"
     gene            17368..18114
                     /gene="rlmB"
                     /locus_tag="P6L01_000712"
     CDS             17368..18114
                     /gene="rlmB"
                     /locus_tag="P6L01_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDP3029800.1"
                     /translation="MEDTVIVGRHAVREAIITGHPINKILIQEGIKKQQINEILKNAK
                     DQKIIVQTVPKSKLDFLANAPHQGVAALIAPYEYADFDQFLKQQKEKEGLSTVLILDG
                     LEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVIRVTNLAKT
                     IDELKDNGFWVAGTEANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPM
                     VGHVNSLNASVAASLMMYEVFRKRHDVGEI"
     gene            18114..18638
                     /locus_tag="P6L01_000713"
     CDS             18114..18638
                     /locus_tag="P6L01_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDP3029801.1"
                     /translation="MKERYLIIDGYNMIGQSPTLSAIAKENLEEARMQLIDAIANYNA
                     VISDEIICVFDAYDQSGVEREYMYHGVKTIFTKEKETADSFIERYVYELYDKHTKHIA
                     VVTSDMSEQHAIFGSGAYRISSREMWRDLKENEIDVSKSLDDISENKPRTRIPLSSEI
                     LAEFEKIRRGHHKK"
     gene            18719..19288
                     /locus_tag="P6L01_000714"
     CDS             18719..19288
                     /locus_tag="P6L01_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDP3029802.1"
                     /translation="MKYDLTTQDSTIKRNNSINDKDFEKLVMDLQPLIIRRIKTFGFN
                     HYDLEDLYQEILIRMYRSVQTFDFSGEQPFTNYVQCLITSVKYDYLRKYLATNKRMDN
                     LINEYRVTYPCAIKRFDVENNYLNKLAIKELICQFKYLSAFEKDVMYLMCEQYKPREI
                     AQLMHVKEKVIYNAIQRCKNKIKRYFKMI"
     gene            19403..19546
                     /gene="rpmG"
                     /locus_tag="P6L01_000715"
     CDS             19403..19546
                     /gene="rpmG"
                     /locus_tag="P6L01_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDP3029803.1"
                     /translation="MRKIPLNCEACGNRNYNVPKQEGSATRLTLKKYCPKCNAHTIHK
                     ESK"
     gene            19602..19784
                     /gene="secE"
                     /locus_tag="P6L01_000716"
     CDS             19602..19784
                     /gene="secE"
                     /locus_tag="P6L01_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDP3029804.1"
                     /translation="MAKKESFFKGVKSEMEKTSWPTKEELFKYTVIVVSTVIFFLVFF
                     YALDLGITALKNLLFG"
     gene            19797..20345
                     /gene="nusG"
                     /locus_tag="P6L01_000717"
     CDS             19797..20345
                     /gene="nusG"
                     /locus_tag="P6L01_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDP3029805.1"
                     /translation="MSEEVGAKRWYAVHTYSGYENKVKKNLEKRVESMNMTEQIFRVV
                     IPEEEETQVKDGKAKTTVKKTFPGYVLVELIMTDESWYVVRNTPGVTGFVGSAGAGSK
                     PNPLLPEEVRFILKQMGLKEKTIDVELEVGEQVRIKSGPFANQVGEVQEIETDKFKLT
                     VLVDMFGRETPVEVEFDQIEKL"
     gene            20526..20948
                     /gene="rplK"
                     /locus_tag="P6L01_000718"
     CDS             20526..20948
                     /gene="rplK"
                     /locus_tag="P6L01_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDP3029806.1"
                     /translation="MAKKVDKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNA
                     RTQDQAGLIIPVEISVYEDRSFTFITKTPPAPVLLKKAAGIEKGSGEPNKTKVATVTK
                     DQVREIANSKMQDLNAADEEAAMRIIEGTARSMGIVVE"
     gene            21156..21848
                     /gene="rplA"
                     /locus_tag="P6L01_000719"
     CDS             21156..21848
                     /gene="rplA"
                     /locus_tag="P6L01_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDP3029807.1"
                     /translation="MAKKGKKYQEAASKVDRTQHYSVEEAIKLAKETSIANFDASVEV
                     AFRLGIDTRKNDQQIRGAVVLPNGTGKSQSVLVFAKGDKIAEAEAAGADYVGEAEYVQ
                     KIQQGWFDFDVVVATPDMMGEVGKLGRVLGPKGLMPNPKTGTVTMDVKKAVEEIKAGK
                     VEYRAEKAGIVHASIGKVSFTDEQLIENFNTLQDVLAKAKPSSAKGTYFKSVAVTTTM
                     GPGVKIDTASFK"
     misc_feature    21936..>22062
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
BASE COUNT         8145 a         3024 c         4221 g         6672 t
ORIGIN      
        1 aaaatggatg cgatgagccg cattgagacc gcaaggtctc ttttttttat gtctaaaacg
       61 tcaaaataaa agttaacaca aagaaaatgg cttgacgaag tgaaaacgtt tgaatctgac
      121 gaaacgagaa aagagcgcaa cgagtttagt agagctaaat gagtaagcga gagccgaaga
      181 agaggaaaga agcaagcgat tgtcacaagt caagaaaggt tcttagcgag gatggtagcc
      241 aacttacgtt ccgctagagt agaactggaa atgataattt aataatgtac actttcgatt
      301 gtctaagtat gtacaacttt aattttgtgt ttatataaat ttaaaatgat atcatcgaaa
      361 acaaaatatt gtataaatag agaagagcag taagacggta tctaattgaa aatgatctta
      421 ctgctctttt atatacttta ttgaaataca aaaaggaaat taattattat acaatagaca
      481 agctattgca taagtaacac taacttttat caaagaagtg ttactttata attaatgatt
      541 ttattagagc gtctacatgc ggttttaaag catcatcgtc tataccgcca aagcctaata
      601 taaatttagg ggttttctta tagtcttgat catcatcaaa attataaact tgtaatttta
      661 actttacttt gtttgctcta tcaagacact cttgtaatgt taatccattt tttactgtaa
      721 ttgtaaaatg catacccgtt tcagcacctt gaatatcaag ctgctctttg taaggtttca
      781 atctttttaa aatataggtt agttttctac gataaattcg tctcatttta tttaaatgcc
      841 tttcaaaacc accggaagat ataaacgttg caataaggtt ttgcatatga acaggtacag
      901 tgttgccttc aatgtgattt tgagaatgat attttttcat tatagaatag ggtaacacca
      961 tatatgcaac tcgacagcta ggaaaaatag actttgaaaa tgtactgata taaatcactt
     1021 tttctcctct tgaatataga ccttgaattg ctggaatggg tttgccgaaa tatctaaact
     1081 cggaatcata atcatcttct ataataaatc gttcttcttt ttcttgagcc cattgtatta
     1141 attgagttcg tttttttaag tccatcacat atccagttgg aaattgatgg gaaggcgtta
     1201 tatatactat atttttttgt gatttaataa cttcatctac gtttattcca ttatcttcaa
     1261 cttcaatttg ttcatattca acttgttttt tatctaaaat atttttgatt ggtggataac
     1321 taggtttttc gataataaac gttgaagtat aaagtaaatc gactaattga tttactaatt
     1381 gttcggtaga tgagccaatt ataatttgat taggatcaca aattacacca cgattagtaa
     1441 ataaataaaa tgccagttga aatcttaaat gtaattctcc ttgaaaatgt cctctacgta
     1501 attgatttaa atgatttgta tcataaagat ctttggaata ctttctgaaa agttctatag
     1561 ggaaatgttt cgtatctatt tcatccaaat taaaagcata atcataagct tcatcactcg
     1621 cttttggttt atatgaatca tcatcaaaaa gagaggggat aggttgattg tttaaaattg
     1681 ttaaagattc aatttcggac acaaaatatc cagagcgagg tcttgaataa atgtaacctt
     1741 cgtctaatag aagttgatat gcatgctcta cggttgtttg gctgatagat aaatgtttgc
     1801 ttaattgtct tttagaataa aatttatcgc cttctttaaa ttgaccttca attatttgtt
     1861 tttttaattt ttcataaagt tgatggtata aagtgttttt caattttata actgacctcc
     1921 taaatttatc ttattttgta cctttttaaa tatcagttta tacattacaa tgtatttaat
     1981 caacttgaaa aggggtttta tgtataatga gtaaaattat tggatcagac agagtcaaaa
     2041 gaggtatggc tgaaatgcaa aaaggcggcg ttattatgga tgtcgttaat gctgagcaag
     2101 caagaattgc agaagaagca ggcgcggtag cagttatggc attagaacga gtaccttctg
     2161 atattagagc tgctggtggc gttgcacgta tggcaaaccc taaaattgta gaagaagtaa
     2221 tgaatgctgt ttctattcca gtcatggcta aagcacgtat tggtcatatc actgaagcaa
     2281 gagtattaga ggcgatgggt gttgactata ttgatgaatc agaagtgtta acaccagcag
     2341 atgaggaata tcacttaaga aaagatcaat ttacagtacc atttgtatgt ggatgtcgta
     2401 atttaggtga agctgcgcgt agaattggtg aaggtgctgc tatgttacgt actaaaggtg
     2461 aaccaggtac aggtaatatt gttgaagctg taagacatat gagacaagtt aattcagaag
     2521 ttagtcgatt gactgtaatg aatgatgatg agattatgac ttttgcgaaa gatatcggtg
     2581 cgccttatga aattttaaaa caaattaaag acaatggtcg tttaccggta gttaactttg
     2641 cagctggtgg cgttgcgact cctcaagatg ctgctttaat gatggaatta ggtgctgacg
     2701 gtgtattcgt tggatcaggt atttttaaat cagaagatcc agaaaaattt gctaaagcaa
     2761 ttgttcaagc aacaacacat taccaagact atgaactaat tggaagatta gcaagtgaac
     2821 ttggcactgc tatgaaaggt ttagatatca atcaattatc attagaagaa cgtatgcaag
     2881 agcgtggttg gtaagatatg aaaataggtg tattagcatt acaaggtgca gtacgtgaac
     2941 atattagaca tattgaatta agtggtcatg aaggtattgc agttaaaaaa gttgaacaat
     3001 tagaagaaat cgagggctta atattacctg gtggcgagtc tacaacgtta cgtcgattaa
     3061 tgaatttata tggatttaaa gaggctttac aaaattcaac tttacctatg tttggtacat
     3121 gcgcaggatt aatagttcta gcgcaagata tagttggtga agaaggatac cttaacaagt
     3181 tgaatattac tgtacaacga aactcattcg gtagacaagt tgacagcttt gaaacagaat
     3241 tagatattaa agggatcgct acagatattg aaggtgtctt tataagagcg ccacatattg
     3301 aaaaagtagg tcaaggcgta gatatcctat gtaaggttaa tgagaaaatt gtagctgtcc
     3361 agcaaggtaa atatttaggc gtatcattcc atcctgaatt aacagatgac tatagagtaa
     3421 ctgattactt tattaatcat attgtaaaaa aagcatagct taatgtatgc taaatcaacg
     3481 aattattgat atttatagat ttgttgagaa gaaaatatct ccttcaaact tagctttgga
     3541 ggagttattt tttatgtcaa aattaaaaat gataaaaaat aaagatatac ataagaaaaa
     3601 accccttcaa agagactgag aatagtcaaa attttgaagg gattaattcg atgttgatgt
     3661 atttgttaaa taaagaatcc agcgattgca gctgaaatga aagatactag tgttgcaccg
     3721 aataataatt tcaaaccaaa gcgggcaact gtatctcctt ttttgtcatt aagtgattta
     3781 atagcacctg aaataatacc aatagagcta aagttagcaa atgatactaa gaatacagat
     3841 gtaacacctt ttgcgtgttc agataaatca ctaagtttac caagtgcttg cattgctaca
     3901 aattcgttag ataatagttt tgtcgccata actgaaccgg cttgaactgc atcttgccat
     3961 ggcacaccga ctaagaatgc aaatggtgca aagacaaaac caattaatgt ttggaaatcc
     4021 caagaaatag cgccacctga aactgtacta aagatattgc ttacaattcc atttaataga
     4081 gcgataatgg caatgtatcc gattaacatt gcacctacaa tgacagctac tttaaatcca
     4141 tctaaaatat attctcctag catttcgaag aatgattgtt gtctttcttc agtttcttca
     4201 actaataatt tgtcatcttc ttcattaact ttataagggt taataattga agcgatgatg
     4261 aaaccaccaa ataagtttaa gacaacagcc gttacaacat atttaggttc aattaaggta
     4321 aagtatgcac cgataattga agcagaaaca gtcgacattg ctgaagctgt taatgtgtat
     4381 aaacgttgct taggtatgta tggtaattgt tttttaattg aaataaatac ttcagattgt
     4441 cctaaaattg ctgcagcaac tgcattgtat gattctaaac gtcccatacc attaatttta
     4501 gaaattaaga atcctaaaac attaatgatt aaaggtaaaa tctttgtgta ttgaaggata
     4561 ccgataatcg ctgaaataaa tacgataggt aataatacac tgaagaagaa tggtggttgc
     4621 ttaggatcga tatattgaat accaccgaat acaaagttaa caccatctgc tgcttttaat
     4681 aataagtagt taaaaccgtt tgaaatacca ccaataacct tgattcccat tgtagtttta
     4741 agcaagataa atgcaaagat aagctgaatt gcaagtaaaa ttcctacata tttccagcga
     4801 atatttttcc tgtctgagct aaatagaaac gcaagtgcta aaaagaagat aattccgata
     4861 atcccaatta gaatatgcat atatttctca ttcctttagt tttttctaca atctatcata
     4921 caataaaatg gaagggctaa catcataaat ttttgaaaat ataaaaacaa attaattgaa
     4981 aaaggtcaaa ataggtcata taatatagtc aaagaaggtc aaaaaggggt gatatacatg
     5041 cacaatatgt ctgacatcat agaacaatac atcaaacgtt tatttgaaga gtcgaatgaa
     5101 gatgtcgttg aaattcagag agcgaatatc gcacagcgtt ttgattgtgt accatcacaa
     5161 ttaaattatg taatcaaaac acgattcact aatgaacatg gttatgaaat cgaaagtaaa
     5221 cgtggtggtg gtggttacat ccgaatcact aaaattgaaa ataaagatgc aacaggttat
     5281 attaatcatt tgcttcagct gattggacct tctatttctc aacaacaagc ttattatatt
     5341 attgatgggc ttttagataa aatgttaata aatgaacgtg aagctaaaat gattcaagca
     5401 gttattgata gagaaacgct atcaatggat atggtttcta gagatattat tagagcaaat
     5461 attttaaaac gtttgttacc agttataaat tattactaaa tgaaatgagg tgttgaagtg
     5521 ctttgtgaaa attgtcaact taatgaagcg gaattaaaag ttaaagttac aagtaaaaat
     5581 aaaacagaag aaaaaatggt gtgtcaaact tgtgctgagg ggcaccatcc gtggaatcaa
     5641 gctaatgaac agcctgaata tcaagaacat caagataatt tcgaagaagc atttgttgtt
     5701 aagcaaattt tacaacattt agctacgaaa catggcatta attttcaaga agtagcgttt
     5761 aaagaagaaa aacgttgccc atcatgtcat atgactttga aagatattgc acatgttggt
     5821 aaatttgggt gtgctaattg ttatgcaaca tttaaagatg acatcattga tatcgtccgc
     5881 agagttcaag gtggacaatt tgagcacgtt ggaaagacac cacattcttc acataaaaag
     5941 atagctttaa agcgaaaaat cgaagaaaag aatgaatatt tgaaaaaact tattgaaatc
     6001 caagattttg aggaagcagc cattgttaga gatgaaatta aagcactaaa agctgagagt
     6061 gaggtgcaac atgatgacgc ataatattca tgataatatc agccaatgga tgaaaagtaa
     6121 tgaagaaaca ccaattgtta tgtcttctag aattcggtta gcgcgtaatt tagaaaatca
     6181 tgtgcatcca ctaatgtatg ctactgaaaa tgatggattt agagttataa atgaggtaca
     6241 agatgccttg ccaaattttg aattaatgcg tcttgatcaa atggatcaac aaagtaaaat
     6301 gaaaatggtt gcaaagcatt tgattagtcc tgaactaata aaacaaccag cagccgcagt
     6361 attagtgaat gatgatgaat ctttaagtgt catgataaat gaagaggacc atattcgtat
     6421 tcaagctatg ggaactgaca cgacattaca ggctttatat aatcaagctt catcaattga
     6481 tgatgaatta gatcgaagcc ttgatataag ttatgatgaa caacttggtt atttaactac
     6541 atgtcctacc aatataggta ctggtatgag agcaagcgtg atgctacatt taccaggtct
     6601 atctattatg aaaagaatga cacggattgc tcaaaccatt aatcgttttg gatatacaat
     6661 cagaggtatt tacggtgaag gttcgcaagt ttatggacat acttatcaag tatccaacca
     6721 acttacactt ggtaaatctg agttagaaat catagaaaca ttaacagaag ttgttaatca
     6781 aatcattcat gatgaaaaac aaatacgaca aaagttagac acttataatc aattagaaac
     6841 acaagaccgt gtttttcgct cgctaggtat tttacaaaac tgtagaatga taactatgga
     6901 agaggcttct tatagattaa gcgaagttaa acttggtata gatttaaatt acattgaatt
     6961 acaaaacttt aaatttaatg aattgatggt agctatacag tcaccatttt tattagatga
     7021 agaagatgac aaatctgtaa aagaaaaacg agcagatata ctaagagaac atataaagta
     7081 ggaggtcatt atttatgtta tttggtagat taactgaacg tgcacagcgc gtattagcac
     7141 atgcacaaga agaagcaatt cgtttaaatc attcaaatat aggaacagaa cacctattat
     7201 tggggttaat gaaagaacct gaaggaattg ctgcaaaagt attagaaagt tttaatatca
     7261 ctgaagataa agtaattgaa gaagttgaaa aattaatcgg acatggtcaa gatcatgttg
     7321 gtacattgca ttatacacct agagctaaaa aagtcattga attatcgatg gatgaagcta
     7381 gaaaattaca tcacaatttt gttggaacgg aacatatttt attaggcttg attcgtgaaa
     7441 atgaaggtgt tgcagcaaga gtttttgcaa atctagattt aaatattact aaagcacgtg
     7501 cacaagttgt gaaagcttta ggaaaccctg aaatgagtaa taaaaatgca caagctagta
     7561 agtcaaataa tactccaact ttagatagtt tagctcgtga cttaacagtc attgccaaag
     7621 acggtacatt agatcctgtt ataggacgtg ataaagaaat tacacgtgta attgaagtat
     7681 taagtagacg tacgaaaaac aatcctgtac ttattggaga gccaggtgtt ggtaaaactg
     7741 ctattgctga aggtttagcg caagccatag tgaataatga ggtaccagag acattaaaag
     7801 ataagcgtgt tatgtcttta gatatgggaa cagtagttgc aggtactaaa tatcgtggtg
     7861 aatttgaaga gcgtctgaaa aaggttatgg aagaaatcca acaagcaggt aatgtcatcc
     7921 tatttattga tgagttgcat actttagttg gtgctggtgg tgctgaaggt gctatcgatg
     7981 cttcgaatat tttgaaaccg gcattagcac gtggtgaatt acaatgtatt ggtgctacta
     8041 cattagatga atatcgcaaa aatattgaaa aagacgcggc tttagaacgt cgtttccaac
     8101 ctgtacaagt tgatgaacct tcagtagtag atacagttgc tattttaaaa ggattaagag
     8161 atcgttacga agcacaccat cgtattaata tttcagacga agctattgaa gcagctgtta
     8221 aattaagtaa cagatacgtt tcagatcgtt tcttaccaga taaagcaatt gatttaattg
     8281 atgaagcaag ttctaaagta agacttaaga gtcatacgac acctaataat ttaaaagaaa
     8341 ttgaacaaga aattgaaaaa gttaaaaatg aaaaagatgc cgcagtacat gctcaagagt
     8401 ttgaaaatgc tgctaacctg cgtgataaac aaacaaaact tgaaaagcaa tatgaagaag
     8461 ctaaaaatga atggaagaat gcacaaaatg gcatgtcaac ttcattgtca gaagaagata
     8521 ttgctgaagt tattgcagga tggacaggta tcccattaac taaaatcaat gaaacagaat
     8581 ctgaaaaact tcttagtcta gaagatacat tacatgagag agttattggg caaaaagatg
     8641 ctgttaattc aatcagtaaa gcggttagac gtgcccgtgc agggttaaaa gatcctaaac
     8701 gaccaattgg tagctttatc ttccttggac caactggtgt tggtaaaact gaattagcta
     8761 gagctttagc tgaatcaatg tttggcgatg atgatgcgat gatccgtgta gacatgagtg
     8821 aatttatgga aaaacacgca gtgagccgat tagttggtgc tcctccagga tatgttggtc
     8881 atgatgatgg tggacaatta actgaaaaag ttagacgtaa accatattct gtaattttat
     8941 ttgatgaaat tgaaaaagct catccagatg tatttaatat tctattacaa gttttagatg
     9001 atggacattt gacagataca aaaggacgta cagttgattt cagaaataca attatcataa
     9061 tgacatcaaa cgttggggca caagaattac aagatcaacg atttgctgga ttcggtggtt
     9121 caagtgatgg acaagattat gaaacaattc gaaaaacgat gttaaaagaa ttaaaaaatt
     9181 cattccgtcc agaattttta aaccgtgtag atgatatcat tgtattccat aaactaacaa
     9241 aagaagaatt aaaagaaatt gtaacaatga tggttaataa attaacaaat cgattatctg
     9301 aacaaaacat aaatattatt gttactgata aagcgaaaga caaaatcgca gaagaaggat
     9361 atgatccaga atatggtgca agaccattaa ttagagcgat acaaaaaact atcgaagata
     9421 atttaagtga attaatatta gatggtaatc aaattgaagg taagaaagtt acagtagatc
     9481 atgatggtaa agagtttaaa tatgacattg ctgaacaaac ttcagaaact aaaacaccat
     9541 cgcaagcata attataaaac agtccaaaac aaattaaagt tttgggctgt ttttttagta
     9601 gcattgaact atagaaattc gtgaaagtat ccatcaacga aacaatctaa taaaacaatc
     9661 atcaaaggat agttaagaat tatatgtaac aagttaatga gcctacagcg atagcataaa
     9721 ggtatgaatt tttataaagg ttttttgttt gaagatacta gcaacttacg tcaaaataaa
     9781 ataggtctat ataatattgt gataaatcta gagaaaagag tttactaaga aattattaag
     9841 attttatctt tgaaagatac cgactatcaa catgatgtaa gtttatttta taatattcat
     9901 aaaaaataaa tctggtaaaa cagtttgcgt tagtagtcat gttaaaatac aaacaaatgt
     9961 aactcacatt taatttgtca taatgggaat gtgcgtttaa aatagatttg ttcaaaggaa
    10021 agtggaggtg caattttggc caagaaaaaa gtgatttttg aatgtatggc ttgtggttat
    10081 caatctccta aatggatggg gaaatgtcct aattgtggcg cttggaatca aatggaggaa
    10141 attgttgaaa aagcagccaa tcctaaacat ggagttaaaa ccaaggaatt agcaggtaaa
    10201 gtacaaaaat taaatagtat taaacatgaa acaacgccga gagtgttaac agattcagca
    10261 gaattcaacc gtgtattagg tggaggtatt gtgagcggat cgttagtact tattggtggg
    10321 gatccaggta ttggtaagtc aacgttactt ttacaaattt gtgcatcgtt atctcaaaag
    10381 aaaaaagtac tatatattac tggagaagaa tcgcttagtc agactaaatt acgtgcagag
    10441 cgattagatg aagattcaag tgaattgcaa gtattagctg aaacagatct tgaagttatt
    10501 tatcaaacag taaaagaaga acaacctgat ttattagtag tggattcgat tcaaacaata
    10561 tatcatcctg aaatcagctc tgcgccaggt tctgtttcac aagttcgtga aagtacacaa
    10621 agtttaatga atattgctaa acaaatgaac attacaactt ttatagtggg tcatgtaacg
    10681 aaagaaggtc aaattgctgg cccaagattg ctagaacaca tggttgatac tgtgctttat
    10741 tttgaaggcg atgaacacca cgcatatcga attttgcgag ctgttaaaaa ccgttttggt
    10801 tcaacgaatg aaatgggaat cttcgaaatg aagcaaagtg gattaaaagg tgtaaataat
    10861 ccatctgaaa tgtttttaga agaacgttca acaaatgttc caggttcaac aattgttgca
    10921 accatggagg gaaccagacc acttttaata gaagttcaag cgctggtaac tccaacgact
    10981 tttaacaatc cgagacgaat ggcaacaggg attgatcata atcgattaag tttgttgatg
    11041 gctgttttgg aaaagaaaga aaattatcta ttacaacaac aagatgctta tatcaaagta
    11101 gctggcggtg taaagttaac ggagccagca gttgatttaa gtgtaattgt agcaactgca
    11161 tctagcttta aagataaagc tgtcgacgga ttagattgct atattggaga agttggttta
    11221 acgggtgagg tacgtcgtgt atctcggata gaacaacgcg tgcaagaggc tgcaaaacta
    11281 ggtttcaaac gtgtaattat tcctaaaaat aatataggcg gatggacata tcctgaaggt
    11341 atacaagtaa taggtgtaac tactgtacat gaagcattgt catttgctct tcattcataa
    11401 aacatcaaga aaggaggaca ttgtgtgaat atcgttaaac taatggttat tattatttac
    11461 ttaattattg ggagcgcatt aggaataatt attattcctg aaattgcaaa tgatcttgga
    11521 ttacaaaact ccagcttttt aaaaaatcac tatgtagatg gcattatcgg tagtattttt
    11581 atgttcttaa tttttggtgt atttattaga cgagttacta acgctataaa aggtttagaa
    11641 cattttatta tgcgtagaag tgctgttgaa atactattcg caacaatagg tttaataatc
    11701 ggattactta tttctgttat ggtgtcgttt atattagaat caattggtaa ctctattttt
    11761 aatcatttca ttcctgtcat aattacgata ttactatgtt atttcggttt ccaatttggc
    11821 cttaaaaaac gagatgaaat gttaatgttt ttacctgaga atatagcgcg ttccatgtca
    11881 caacatacta aaagtgctac gccaaaaatt atcgacacaa gcgcaattat tgatggtcgt
    11941 attttagaag tcattcgttg cggttttatc gatggcaata ttttaattcc gcaaggtgtt
    12001 attaatgaat tacaaattgt tgcagattca aatgacagtg ttaaacgtga aaagggtaaa
    12061 agaggcttag atattttaaa tgaattgtat gatttagatt atcctacaaa ggttatacat
    12121 ccaactaaaa cacatagtga tattgataca atgctattaa agctagctaa acaatatcat
    12181 gcaagtatta ttacgacaga tttcaaccta aataaagttt gtcatgtaca tggtatcaaa
    12241 gcattaaatg ttaatgattt atcagaagca atcaaaccta atgtacatca aggtgatcaa
    12301 ctgcatattt tactgacaaa aatgggtaaa gagcctggtc aggcagtagg atatctagat
    12361 gatggtacga tggtagttgt tgataatgct aaaaatctta ttggcagtca tgtcaattta
    12421 gaagtagtca gcttattgca aacatcttca ggaagaattg tttttgctaa aaaaatcgaa
    12481 gatacagtat cattataaaa actgtagttg acgaaaacaa aatagatatt aaaaggtata
    12541 tgaaatggcg aagtttgtaa tttgaagtat aactataaat aaatgcaaaa catatcagtt
    12601 atcaaaaaat acaaatagtg ctaatatatc attgaaacaa gttcattaag tgttaaattt
    12661 tagatattaa taaagttatt cttatagcaa tcaagacata caaaattaga atcaatttta
    12721 acgtatttga atagttaatt tgaagtttaa ttttacagta cataatattc atttttaagt
    12781 cgttgaaatg cttatgtagt gcagtcttga ttcttaggga tagctttttt aaagtgttga
    12841 aaaaacaact gctttctatt taaaatttaa gcatattacc cagtgaatta tgttatgatt
    12901 aaacaatgaa attattttaa ggtcatgtta aaaagcattg tttcgtttta acaataagac
    12961 gagttagaca tttgttatga cggtcgaaga tagcaaatga ataatttata tgaactaact
    13021 tgaaaatctg atatttaatt taggagtgaa aaaaatgagc gatcgtataa gagtaagata
    13081 tgcaccaagt ccaactggtt atcttcatat tggtaatgca agaacagcat tattcaatta
    13141 cttgtatgct aaacattaca acggagattt tgtgattcga attgaagata ctgataaaaa
    13201 acgtaattta gaagatggag aaacatcaca atttgataat cttaaatggt taggattaga
    13261 ttgggatgag tctgtagata aagacaatgg ctacggacca tatcgtcaat ctgaacgtca
    13321 acatatctac caaccattaa tagatcagtt actagcagaa gataaagcat ataaatgcta
    13381 tatgacagaa gaagaattag aagctgaacg tgaagcgcaa atcgctcgtg gtgaaatgcc
    13441 tcgctatggt ggtcaacatg cgcatttgac tgaagaacaa cgtcaacaat ttgaagcaga
    13501 aggacgccaa ccatcaattc gtttccgagt acctcaaaac caaacgtatt catttgatga
    13561 tatggtaaaa ggaaatattt catttgattc aaatggtatt ggtgactggg ttatcgtaaa
    13621 aaaagatggc attccaacgt acaattttgc agtagctata gatgatcatt acatgcaaat
    13681 ttcagatgta attcgtggtg atgatcatat ttcaaacacg cctaaacaaa ttatgattta
    13741 tgaagcattt ggctgggagc cacctcgttt tggtcatatg tcattaattg ttaatgaaga
    13801 acgtaaaaag ttaagtaaac gtgatgggca aattttacaa tttattgagc aatatcgtga
    13861 cttaggttat ttacctgaag cgttatttaa ttttattgcg ttattaggtt ggtctcctga
    13921 aggtgaagaa gaaatctttt ctaaagaaga atttatcaaa atctttgatg aaaagcgttt
    13981 gtcaaaatca ccagcatttt tcgataagca aaaattagca tgggttaata accaatatat
    14041 gaaacaaaaa gatactgaaa cagtattcca attagcatta cctcatttaa ttaaagcaaa
    14101 tttgattcct gaggtgccgt cagaagagga tttatcttgg ggacgcaaat taattgcgct
    14161 ttatcaaaaa gaaatgagtt atgccggtga aattgtacct ttatcagaaa tgttctttaa
    14221 agaaatgcca gctcttggtg aagaagaaca acaagtgatt aatggagagc aagtaccaga
    14281 gttaatgacg cacttattca gtaaattaga agcacttgaa ccatttgaag cggctgaaat
    14341 taaaaagaca attaaagaag ttcaaaaaga aacaggaata aaaggcaagc aattatttat
    14401 gcctattcgt gttgctgtaa caggccaaat gcatggtcct gaattaccaa atacaattga
    14461 agtacttggt aaagaaaaag tgctaaaccg tttaaaacaa tataagtaat gaaatagaca
    14521 agatcaaggt agtatatact tgaattttat aacgtaacca catatgataa agttgtatta
    14581 acaaattaca acaatatgaa taaagtattt tatataaaac gaaggattag taattaaatt
    14641 tatacgatgc agagagtgta cggttgctgt gagtacaacg tagaaattaa tgaatgcacc
    14701 ttcgtaaaat gaattaaata tataatgaga gtgatgagca ttaagttgac ttagtttcct
    14761 tgataatttg gaagcgcccg caatattatt aatgttattc gctaaattca gagtggaacc
    14821 gtgcggaagc gcctctaaca atacaatttg tatgttagtg gtgctttttt gatatttaat
    14881 ttcgcaggta cttcaattat gtatttacgt agttcaatca ttgaggagga aatgatcttg
    14941 ttaaaaagaa tgagagacga tataaaaatg gtatttgagc aggatccagc ggcacgttca
    15001 acattagaag tcattacaac gtatgcaggt ttacatgcag tttggagtca tttgattgca
    15061 cataagttat acaaccaaaa aaaatatgtt gcagcacgcg cgatatctca aatttcaaga
    15121 tttttcacag gtatagaaat ccatccaggt gctaaaattg gaaagcgtct atttatagat
    15181 catggtatgg gcgttgtaat aggagaaaca tgtacaattg gtgataatgt gacaatctat
    15241 caaggcgtga cacttggtgg gacagggaaa gaaagaggga aaagacaccc agatatagga
    15301 gacaatgttt taatagcagc cggtgcgaaa gttttaggaa atattaaaat aaattcaaat
    15361 gtaaatattg gtgcaaattc agttgtttta caatcagttc caagttattc aacggttgtt
    15421 ggtataccag gacatattgt taagcaagat ggtgttcgag ttggaaaaac atttgatcat
    15481 cgccatctac ctgatccaat ttatgaacaa attaagcatt tagaacgaca acttgaaaag
    15541 actaggaatg gagagattca agatgattac attatataat acgcttacac gtcaaaaaga
    15601 agtgttcaag cctatagaac cagggaaagt aaaaatgtat gtatgtggtc ctactgtata
    15661 taactacatt catattggta acgcaagacc agcaattaat tatgacgtag tgagacgtta
    15721 ctttgaatac caaggatata atgtagaata tgtatcaaat tttacagacg tagatgataa
    15781 attaattaaa cgttctcaag aattaaatca gtctgttccc gaaattgcag aaaaatatat
    15841 cgctgctttt catgaagatg ttggtgcgtt aaatgttaga aaagcgactt caaatccaag
    15901 ggtaatggac catatggatg acattattca atttattaaa gatttggtgg atcaaggtta
    15961 tgcatatgaa agtggtggcg atgtttactt tagaacacgt aaatttgaag gttatggtaa
    16021 attaagtcat caatccatag atgacttaaa agtgggtgct cgtatagatg caggagagca
    16081 taaagaagat gcacttgatt ttacattgtg gaaaaaagcg aagcctggcg agattagttg
    16141 gaatagccca tttggtgaag gtagaccagg atggcatata gaatgttctg taatggcatt
    16201 tcatgagcta ggacctacaa ttgatataca tgcgggtggt tcagatttac aatttccaca
    16261 tcatgaaaat gaaatagcac aatcagaagc acataatcat gcgccatttg ctaattattg
    16321 gatgcataat ggtttcatta atattgataa tgaaaaaatg agtaaatcac taggcaactt
    16381 tattttagtt cacgatatta ttaaagaagt tgatccagat gtactaagat tctttatgat
    16441 tagcgtacat tatagaagcc caattaacta taatctagaa ttggtagaat cagcacgtag
    16501 tggactagag cgtattcgca atagttatca attaattgaa gagcgcgcac aaattgctac
    16561 taatattgaa aatcaacaga catatattga tcaaattgat gcgattttaa atcgttttga
    16621 aacagttatg aatgatgatt ttaatacagc taatgcaatt acagcttggt atgatttagc
    16681 aaaacttgcg aataaatatg tactagagaa cacaacatca acagaagtaa ttgataaatt
    16741 taaagcagtt tatcaaattt tcagcgatgt tttaggtgta ccgttaaaat ctaaaaatgc
    16801 agatgaatta ttggatgaag atgttgaaaa attaatcgaa gagcgtaatg aagcaaggaa
    16861 aaacaaagat tttgcacgag cagatgagat tcgagacatg ctgaaatcac aaaacattat
    16921 attagaagac acacctcaag gggttagatt taaacgtgga taatcaacaa gataatcaca
    16981 ttaaattatt gaatccattg accttagcat atatgggaga cgcagtctta gatcaatatg
    17041 tacgtaccta tatcgtttta aagcttaaaa gtaagcctaa taaactacat caaatgtcta
    17101 aaaaatatgt atctgccaaa agtcaggcgc aaacgttaga atatttaatg gagcaagaat
    17161 ggtttacaga cgaagaaatg gatattttga agcgagggcg taacgcgaaa agtcatacta
    17221 aagctaaaaa cactgatgtt caaacatatc gtaaaagttc agcgatagaa gcagtgatag
    17281 gttttcttta tttagaaaaa agagaagaac gattagaggc attattaaat aaaataataa
    17341 caatagtaaa cgaaaggtag tgacgatgtg gaagatacgg ttattgttgg taggcatgct
    17401 gttagagaag cgattattac tgggcatccg ataaataaga tattgattca agaaggtatt
    17461 aaaaagcaac aaattaatga aattttaaaa aatgcaaaag atcaaaaaat tattgttcaa
    17521 actgtaccaa aatctaaatt agatttttta gcaaatgcac cacatcaggg tgttgcagcg
    17581 cttattgcac catatgaata tgctgacttc gatcaatttt taaaacagca aaaagaaaaa
    17641 gaaggtttat cgacagtact tatattagac ggcttagaag acccgcataa cttgggatca
    17701 attttaagaa cagccgatgc aacgggagtt gatggtgtta ttattcctaa acgtcgttca
    17761 gttacactaa cgcaaacagt tgcaaaagcc tcaacaggtg caattgaaca tgtaccagtt
    17821 attcgagtga caaatttagc taaaactatc gatgaactaa aagataatgg cttttgggta
    17881 gctggcactg aagctaataa tgcaacagat tatagaaatc tagaagcgga catgtcattg
    17941 gctattgtaa ttggtagcga aggacagggt atgagtcgcc tagtaagtga taaatgcgat
    18001 ttttatatta agattccaat ggttggacat gtaaacagtt tgaatgcttc ggttgcagca
    18061 agtttaatga tgtacgaagt atttcgaaaa agacatgatg ttggagaaat ataatgaaag
    18121 aacgttactt aatcattgat ggatacaata tgataggaca atcaccaacg ctaagcgcca
    18181 ttgcaaaaga gaatttagaa gaagctagaa tgcaattaat agatgcaatt gcaaattata
    18241 atgcagttat ttcagatgaa attatttgtg ttttcgatgc ttatgaccaa tcgggtgttg
    18301 aaagagaata catgtatcat ggcgttaaaa cgatttttac caaggaaaaa gaaacagctg
    18361 atagtttcat agaacgttat gtttatgaac tttatgacaa gcatactaag catattgcag
    18421 ttgtaacaag tgatatgagt gagcaacatg ctatctttgg atcaggtgca tatagaatat
    18481 catctcgcga aatgtggaga gatttaaaag aaaatgaaat tgatgtgagt aaatcattag
    18541 atgatataag tgaaaacaag ccaagaactc gaattccgtt atcttctgaa atccttgcag
    18601 aatttgaaaa aatacgaaga ggacatcata agaaatgaca tttccgtcat cttgaaattt
    18661 tgaatgtaac aatattaaac taaccttaaa ttttagatag aaggggttag tttaatactt
    18721 gaaatatgat ttgacaactc aagacagtac aatcaaacgt aacaattcaa taaatgataa
    18781 agacttcgaa aagttagtaa tggatctaca accattaatt attcgacgca tcaaaacatt
    18841 tggatttaat cattatgatt tagaagactt atatcaagaa atacttatac ggatgtatag
    18901 gtcggtccaa acatttgatt ttagtggaga gcagcctttc acaaattatg ttcaatgttt
    18961 aattacgtct gtaaagtatg attatttgag aaaatattta gctacaaata aaagaatgga
    19021 taatttgatt aatgaatata gagttacgta tccatgtgca ataaagcgtt ttgatgttga
    19081 aaacaattat ttgaataaat tagcaattaa agagttgatt tgtcagttta agtatttgag
    19141 tgcatttgaa aaagatgtca tgtatttaat gtgtgaacaa tataagccga gagaaattgc
    19201 tcaattgatg catgtaaaag agaaagtgat ttataatgcc atacaacgat gtaaaaataa
    19261 aataaaacgt tatttcaaaa tgatttgaaa agcgccttag gacgtgaatt gaattataac
    19321 gtgttactta ctgatggttt gacatttgtt ataaatttta tgtatagtat actggtatta
    19381 taatgaataa aggtgaatta ttgtgagaaa aataccttta aattgtgaag cttgtggcaa
    19441 tagaaattat aatgttccta agcaagaagg ctcggcaaca agattaacct taaagaaata
    19501 ttgtccaaaa tgtaacgcgc acacaattca taaagaatcg aaataaatac attcgaaata
    19561 atactttgat aatatgttca aaggatttgg aggttgagca gatggctaaa aaagaaagtt
    19621 tctttaaagg cgttaagtct gaaatggaaa aaacaagttg gccgacgaaa gaagagctat
    19681 ttaaatatac tgtaattgta gtttctactg ttatattctt cttagtcttt ttctatgcct
    19741 tagatttagg aattacagca ttgaaaaatt tattatttgg ttagaggagt gaagacatgt
    19801 ctgaagaagt tggcgcaaag cgttggtatg cagtgcatac atattctgga tatgaaaata
    19861 aagttaaaaa gaatttagaa aaaagagtag aatctatgaa tatgactgaa caaatcttta
    19921 gagtagtcat accggaagaa gaagaaactc aagtaaaaga tggcaaagct aaaacgactg
    19981 ttaaaaaaac attccctgga tatgttttag tggaattaat catgacagat gaatcatggt
    20041 atgtggtaag aaatacacca ggcgttactg gttttgtagg ttctgcaggt gcagggtcta
    20101 agccaaatcc attgttacca gaagaagttc gcttcatctt aaaacaaatg ggtcttaaag
    20161 aaaagactat cgatgttgaa ctcgaagttg gcgagcaagt tcgtattaaa tcaggtccat
    20221 ttgcgaatca agttggtgaa gttcaagaaa ttgaaacaga taagtttaag ctaacagtat
    20281 tagtagatat gtttggccga gaaacaccag tagaagttga attcgatcaa atagaaaagc
    20341 tttaattaac aattaaagtt attaaactaa ccaaaagata aaaaagagta ttgatttttt
    20401 aattagaaaa gtgttaaaat tatgtggtcg cgcttttaga gcgcccattt cgtcacgaaa
    20461 tgttaagagt gggagggcaa aactgagccc tgtgaccaca tcacgatatc aaggaggtgc
    20521 acatcgtggc taaaaaagta gataaagttg ttaaattaca aattcctgca ggtaaagcga
    20581 atccagcacc accagttggt ccagcattag gtcaagcagg tgtgaacatc atgggattct
    20641 gtaaagagtt caatgcacgt actcaagatc aagcaggttt aattattccg gtagaaatca
    20701 gtgtttatga agatcgttca tttacattta ttacaaaaac tccaccggct ccagtattac
    20761 ttaaaaaagc agctggtatt gaaaaaggtt caggcgaacc aaacaaaact aaagttgcta
    20821 cagtaactaa agatcaagta cgcgaaattg ctaacagcaa aatgcaagac ttaaacgctg
    20881 ctgacgaaga agcagctatg cgtattatcg aaggtactgc acgtagtatg ggtatcgttg
    20941 tagaataatt ttacgaatat taaatttgat tacatgattt aaacgatgaa gcagataaca
    21001 gagataataa tgatgaatta taaatataat ctgaatgact agattaatga ttgatttatt
    21061 cataagatta attcttctgt tgtctgctct taacttgcat atagcaagta atgtgggagg
    21121 aaattccgct aaaaccacta aaggaggaac tataaatggc taaaaaaggt aaaaagtatc
    21181 aagaagcagc tagtaaagtt gaccgtactc agcactacag tgttgaagaa gcaattaaat
    21241 tagctaaaga aacaagcatt gctaactttg acgcttctgt tgaagttgca ttccgtttag
    21301 gaattgatac acgtaaaaat gaccaacaaa tccgtggtgc agttgtatta ccaaacggaa
    21361 ctggtaaatc acaaagtgta ttagtattcg ctaaaggtga caaaattgct gaagctgaag
    21421 cagcaggtgc tgactatgta ggtgaagcag aatacgttca aaaaatccaa caaggttggt
    21481 tcgacttcga tgtagtagtt gctacaccag acatgatggg tgaagttggt aaattaggtc
    21541 gtgtattagg accaaaaggt ttaatgccaa accctaaaac tggaactgta acaatggatg
    21601 ttaaaaaagc tgttgaagaa atcaaagctg gtaaagtaga atatcgtgct gaaaaagctg
    21661 gtatcgtaca tgcatcaatt ggtaaagttt catttactga tgaacaatta attgaaaact
    21721 tcaatacttt acaagatgta ttagctaaag ctaaaccatc atctgctaaa ggtacatact
    21781 tcaaatctgt tgctgtaact acaacaatgg gtcctggagt taaaattgat actgcaagtt
    21841 tcaaataata aatgatataa acaattacag gctgaaagaa atatctttca gtctgtaaaa
    21901 atatattgac aataagtaat ttccaagtta tattacttat tgtgattatt ttacctaaga
    21961 cagtaggagt tatttataac ttaaaattta tcctgccgag gctaaaattg acttgaacgt
    22021 gatgatctat gatctttcaa gcactttttg ccgtgggtag aa
//
LOCUS       DAOBBH010000026        21272 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.026, whole genome shotgun sequence.
ACCESSION   DAOBBH010000026 DAOBBH010000000
VERSION     DAOBBH010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21272
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.026"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            164..1168
                     /gene="aaa"
                     /locus_tag="P6L01_000720"
     CDS             164..1168
                     /gene="aaa"
                     /locus_tag="P6L01_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin/adhesin Aaa"
                     /protein_id="HDP3029808.1"
                     /translation="MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKY
                     GISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLS
                     LIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNAASNSSRPSTNSGGGSYY
                     TVQAGDSLSLIASKYGTTYQKIMSLNGLNNFFIYPGQKLKVTGNASTNSGSATTTNRG
                     YNTPVFSHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRPT
                     VGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYIH
                     "
     gene            complement(1353..1622)
                     /locus_tag="P6L01_000721"
     CDS             complement(1353..1622)
                     /locus_tag="P6L01_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029809.1"
                     /translation="MAKRSKSQRLSSLLNVAGFIVDGYNGYKYHAKNKKLVYLSLGLS
                     TVGTVLDFYISIKSPRKFKKAVAVVTLITNGARLFTSIRKVKHEY"
     gene            1771..2166
                     /locus_tag="P6L01_000722"
     CDS             1771..2166
                     /locus_tag="P6L01_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDP3029810.1"
                     /translation="MIKCVCLVEETADKILLVQVRNREKYYFPGGKIEEGESQVHALL
                     REVKEELNLTLTMDEIEYIGTIVGPAYPQQDMLTELNGFRALTKIDWENVTINNEITD
                     IRWIDKDNDALIAPAVKVWIETYGGKHDK"
     gene            2156..2641
                     /locus_tag="P6L01_000723"
     CDS             2156..2641
                     /locus_tag="P6L01_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDP3029811.1"
                     /translation="MTNNDTIMLRHYVPQDYSMLEAFQLSESDLKFVKTPEENITAAM
                     SDNERYPIVVMDGRQCVAFFTLHRGKGVAPFSDNQDAVFFRSFSVDQRYRNRGIGKVV
                     MEKLASFITSTFQDINEIVLTVNTDNPHAMALYRQQGYQYMGDSMFIGRPVHIMALTI
                     K"
     gene            complement(2810..3592)
                     /locus_tag="P6L01_000724"
     CDS             complement(2810..3592)
                     /locus_tag="P6L01_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDP3029812.1"
                     /translation="MNAVFILALLLLFLMIIFGGKKGFISYLTLFLNFAILIISIVLI
                     IFGVPIYVVTFFFCIIIGACNLFVLNSYNVKTQAAFISTIVTTILLITLIYLSVYFGH
                     LQGFSTEQQDETYVYSMNIGIDMVQFMVFTIILAVIAAVIDLTITISSPIYELHQVNP
                     TLTQYELFQSGMRVGREILATSANTIYLAFFGGQLALFIWFFKLKYSFGHIINSKIFA
                     QEFISIILGGIAVAISIPITAWITAFLIKRPSRQRKNDESIN"
     gene            complement(3589..4707)
                     /locus_tag="P6L01_000725"
     CDS             complement(3589..4707)
                     /locus_tag="P6L01_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDP3029813.1"
                     /translation="MFLKYRNLFTKPFNIILGLFCIAFIGAILFTFNNENFYNKPIGQ
                     IIDVKHVSSTPTKDAQNNRDIKYKNQLKVKILNGQFAGETTTINHQYVKSQADSEAFR
                     THEKVLLHISNKPSDAYIIEKKRDTLTVIITGLFLLTVLLVGRKVGLQSILSLILNSI
                     AILIAIYIHIQHSNINLFLLMTIAMICSTILTLLLVTGWHMRTLITIASTIIGTFLSI
                     GLTELIIYMTDGKGIKYETMNFLSLPPKDIFLASVLIGSLGAIMDVAITIASGMHEIL
                     QRTPHISMRRWALAGRNIGQDIMGTMTNILLFSYLSGALPMFLIFLKNANTVTYTISM
                     NWSLEIARALTGGIGIVLTIPITILFMEIFETLRRAKQ"
     gene            complement(4822..5706)
                     /locus_tag="P6L01_000726"
     CDS             complement(4822..5706)
                     /locus_tag="P6L01_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDP3029814.1"
                     /translation="MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELN
                     VSLFKKQGRNITLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYDESDV
                     SHMLLPLIQTFHLQNDTHVIPSLLDHDTIINSVLNGNIDIGFTELTPEIRKHKQLHML
                     PLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLAPFAETVKKQLRKITKSDVYTI
                     SSQPLAQYLLRQKEGYIISSQNIHLPESKSWIDIKLDHTELKRTICAITKEPYTKSDI
                     GILLTLIQQLMTKTSTFH"
     gene            5887..10386
                     /gene="gltB"
                     /locus_tag="P6L01_000727"
     CDS             5887..10386
                     /gene="gltB"
                     /locus_tag="P6L01_000727"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HDP3029815.1"
                     /translation="MHNEKLIKGLYDYREEHDACGIGFYANMDNKRSHDIIDKSLEML
                     RRLDHRGGVGADGITGDGAGIMTEIPFAFFKQHVTDFDIPGEGEYAVGLFFSKERILG
                     SEHEAVFKKYFEGEGLSILGYRNVPVNKDAIAKHVADTMPVIQQVFIDIRGIEDVEKR
                     LFLARKQLEFYSTQCDLELYFTSLSRKTIVYKGWLRSDQIKKLYTDLSDDLYQSKLGL
                     VHSRFSTNTFPSWKRAHPNRMLMHNGEINTIKGNVNWMRARQHKLIETLFGEDQHKVF
                     QIVDEDGSDSAIVDNALEFLSLAMEPEKAAMLLIPEPWLYNEANDANVRAFYEFYSYL
                     MEPWDGPTMISFCNGDKLGALTDRNGLRPGRYTITKDNFIVFSSEVGVVDVPESNVAF
                     KGQLNPGKLLLVDFKQNKVIENNDLKGAIAGELPYKAWIDNHKVDFDFENIQYQDSQW
                     KDETLFKLQRQFAYTKEEIHKYIQELVEGKKDPIGAMGYDAPIAVLNERPESLFNYFK
                     QLFAQVTNPPIDAYREKIVTSELSYLGGEGNLLAPDETVLDRIQLKRPVLNESHLAAI
                     DQEHFKLTYLSTVYEGDLEDALEALGREAVNAVKQGAQILVLDDSGLVDSNGFAMPML
                     LAISHVHQLLIKADLRMSTSLVAKSGETREVHHVACLLAYGANAIVPYLAQRTVEQLT
                     LTEGLQGTVVDNVKTYTDVLSEGVIKVMAKMGISTVQSYQGAQIFEAIGLSHDVIDRY
                     FTGTQSKLSGISIDQIDAENKARQQSDDNYLASGSTFQWRQQGQHHAFNPESIFLLQH
                     ACKENDYAQFKAYSEAVNKNRTDHIRHLLEFKACTPIDIDQVEPVSDIVKRFNTGAMS
                     YGSISAEAHETLAQAMNQLGGKSNSGEGGEDAKRYEVQVDGSNKVSAIKQVASGRFGV
                     TSDYLQHAKEIQIKVAQGAKPGEGGQLPGTKVYPWIAKTRGSTPGIGLISPPPHHDIY
                     SIEDLAQLIHDLKNANKDADIAVKLVSKTGVGTIASGVAKAFADKIVISGYDGGTGAS
                     PKTSIQHAGVPWEIGLAETHQTLKLNDLRSRVKLETDGKLLTGKDVAYACALGAEEFG
                     FATAPLVVLGCIMMRVCHKDTCPVGVATQNKDLRALYRGKAHHVVNFMHFIAQELREI
                     LASLGLKRVEDLVGRTDLLQRSSTLKANSKAASIDVEKLLCPFDGPNTKEIQQNHNLE
                     HGFDLTNLYEVTKPYIAEGRRYTGSFTVNNEQRDVGVITGSEISKQYGEAGLPENTIN
                     VYTNGHAGQSLAAYAPKGLMIHHTGDANDYVGKGLSGGTVIVKAPFEERQNEIIAGNV
                     SFYGATGGKAFINGSAGERFCIRNSGVDVVVEGIGDHGLEYMTGGHVINLGDVGKNFG
                     QGMSGGIAYVIPSDVEAFVENNQLDTLSFTKIKHQEEKAFIKQMLEEHVSHTNSTRAI
                     HVLKHFDRIEDVVVKVIPKDYQLMMQKIHLHKSLHDNEDEAMLAAFYDDSKTIDAKHK
                     PAVVY"
     gene            10404..11867
                     /locus_tag="P6L01_000728"
     CDS             10404..11867
                     /locus_tag="P6L01_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HDP3029816.1"
                     /translation="MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGA
                     RCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDLVYHQDFKTAYERLSETNNFPDFT
                     GRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGS
                     GPAGLTAAEELNLLGYQVTIYERARESGGLLMYGIPNMKLDKDVVRRRIKLMEEAGIT
                     FINGVEVGVDIDKATLESEYDAIILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQL
                     LNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQFNKYTKLPEAITFTE
                     NASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGE
                     NGMVMKEGPERFWPADLVLLSIGFEGTEPTVPNAFNIKTDRNRIVADDTNYQTNNEKV
                     FAAGDARRGQSLVVWAIKEGRGVAKAVDQYLASKVCV"
     gene            12136..12228
                     /locus_tag="P6L01_000729"
     tRNA            12136..12228
                     /locus_tag="P6L01_000729"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12172..12174,aa:Ser,seq:tga)
     gene            12697..14124
                     /gene="treP"
                     /locus_tag="P6L01_000730"
     CDS             12697..14124
                     /gene="treP"
                     /locus_tag="P6L01_000730"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDP3029817.1"
                     /translation="MAVKREDVKAIVSAIGGKENLEAATHCVTRLRLVLKDESKVDKD
                     ALSNNALVKGQFKADHQYQIVIGPGTVDEVYKQFIDETGAQEASKDEAKQAAAKKGNP
                     VQRLIKLLGDIFIPILPAIVTAGLLMGINNLLTMKGLFGPKALIEMYPQIADISNIIN
                     VIASTAFIFLPALIGWSSMRVFGGSPILGIVLGLILMHPQLVSQYDLAKGNIPTWNLF
                     GLEIKQLNYQGQVLPVLIAAYVLAKIEKGLNKVVHDSIKMLVVGPVALLVTGFLAFII
                     IGPVALLIGTGITSGVTFIFQHAGWLGGAIYGLLYAPLVITGLHHMFLAVDFQLMGSS
                     LGGTYLWPIVAISNICQGSAAFGAWFVYKRRKMVKEEGLALTSCISGMLGVTEPAMFG
                     VNLPLKYPFIAAISTSCVLGAIVGMNNVLGKVGVGGVPAFISIQKEFWPVYLIVTAIA
                     IVVPCILTIVMSHFSKQKAKEIVED"
     gene            14188..15828
                     /gene="treC"
                     /locus_tag="P6L01_000731"
     CDS             14188..15828
                     /gene="treC"
                     /locus_tag="P6L01_000731"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDP3029818.1"
                     /translation="MSKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLL
                     GVDYIWLTPVYESPMNDNGYDISNYLEINEAFGTMDDFEKLIKVAHQKDLKVMLDIVI
                     NHTSTEHEWFKEARKSKDNPYRDYYFFRSSEDGPPTNWHSKFGGNAWKYDSETDEYYL
                     HLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKE
                     FYTDGPRVHEFLHELNRQTFGNTDMMTVGEMSSTTIENCIKYTQPERQELNSVFNFHH
                     LKVDYVDGEKWTNAKLDFHKLKEILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDD
                     TSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLL
                     SEGHAEADVLAILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDISENYHQVNVRQALQ
                     NKESIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSAS
                     AVDLPEGLAREGCVVIQTGTVENNTISGFGAIVIETNA"
     gene            15853..16581
                     /gene="treR"
                     /locus_tag="P6L01_000732"
     CDS             15853..16581
                     /gene="treR"
                     /locus_tag="P6L01_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDP3029819.1"
                     /translation="MAKQKKFMKIYEALKEDILNGQIQYGEQIPSEHDLVQLYQSSRE
                     TVRKALDLLALDGMIQKIHGKGSLVIYQEVTEFPFSELVSFKEMQEEMGVAYLTEVVV
                     NEVVEAHEVPEVQHALNINSSESLIHIVRTRRLNQHVKIVDEDYFLKSIVSDIGNDVA
                     SDSIYDYLEKVLNLNISYSSKSITFEPFDEQAYQLFGDVSVAYSATVRSIVYLENTMP
                     FQYNISKHLANEFKFNDFSRRRIK"
     gene            16643..16912
                     /gene="ffs"
                     /locus_tag="P6L01_000733"
     ncRNA           16643..16912
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="P6L01_000733"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            16985..17086
                     /locus_tag="P6L01_000734"
     CDS             16985..17086
                     /locus_tag="P6L01_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029820.1"
                     /translation="MKTIQRIIRGTCLWEVAFLYVKFDSSELDVQFE"
     gene            17224..17748
                     /locus_tag="P6L01_000735"
     CDS             17224..17748
                     /locus_tag="P6L01_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDP3029821.1"
                     /translation="MQIYLSTLTELDYDKSLNSIEESFDDNPETSWQARAKVKHLRKS
                     PCYNFELEVIAKNENNDVVGHVLLIEVEINSDDKTYYGLAIASLSVHPELRGQKLGRG
                     LVQAVEERAKAQEYSTVVVDHCFDYFEKLGYQNAAEHDIKLESGDAPLLVKYLWDNLT
                     DAPHGIVKFPEHFY"
     gene            17817..19514
                     /gene="dnaX"
                     /locus_tag="P6L01_000736"
     CDS             17817..19514
                     /gene="dnaX"
                     /locus_tag="P6L01_000736"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDP3029822.1"
                     /translation="MNYQALYRMYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFS
                     GPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVD
                     EIRNIRDKVKYAPSESKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPH
                     KIPPTIISRAQRFDFKAISLDQIVERLKFVADAQQIECEDEALAFIAKASEGGMRDAL
                     SIMDQAIAFGDGTLTLQDALNVTGSVHDEALDHLFDDIVQGDVQASFKKYHQFITEGK
                     EVNRLINDMIYFVRDTIMNKTSEKDTEYRALMNLELDMLYQMIDLINDTLVSIRFSVN
                     QNVHFEVLLVKLAEQIKGQPQVIANVAEPAQIDSSPNTDVLLQRMEQLEQELKTLKAQ
                     GVSVAPAQKSSKKPARGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKN
                     NDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNV
                     KVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEE"
     gene            19604..19921
                     /locus_tag="P6L01_000737"
     CDS             19604..19921
                     /locus_tag="P6L01_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDP3029823.1"
                     /translation="MRGGGNMQQMMKQMQKMQKKMAQEQEKLKEERIVGTAGGGMVAV
                     TVTGHKEVVDVEIKEEAVDPDDIEMLQDLVLAATNEAMNKADELTQERLGKHTQGLNI
                     PGM"
     gene            19928..20524
                     /gene="recR"
                     /locus_tag="P6L01_000738"
     CDS             19928..20524
                     /gene="recR"
                     /locus_tag="P6L01_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDP3029824.1"
                     /translation="MHYPEPISKLIDSFMKLPGIGPKTAQRLAFHTLDMKEDDVVQFA
                     KALVDVKRELTYCSVCGHITENDPCYICEDKQRDRSVICVVEDDKDVIAMEKMREYKG
                     LYHVLHGSISPMDGIGPEDINIPSLIERLKSDEVSELILAMNPNLEGESTAMYISRLV
                     KPIGIKVTRLAQGLSVGGDLEYADEVTLSKAIAGRTEM"
     gene            20729..20834
                     /locus_tag="P6L01_000739"
                     /pseudo
     CDS             20729..20834
                     /locus_tag="P6L01_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7117 a         3058 c         4487 g         6610 t
ORIGIN      
        1 aaggggtaat tataaaattt gatgatacag tatatgattt ttttgtaatc ataatgtcat
       61 caaacatcaa cctattatac ataataaaat cgtataatga tgtagtattc ataaattcgg
      121 ataaaagaat gttaggaaag ttaagcaaga ggaggatttt aaagtgcaaa aaaaagtaat
      181 tgcagctatt attgggacaa gcgcgattag cgctgttgcg gcaactcaag caaatgcggc
      241 tacaactcac acagtaaaac cgggtgaatc agtgtgggca atttcaaata agtatgggat
      301 ttcgattgct aaattaaagt cattaaacaa tttaacatct aatctaattt tcccaaacca
      361 agtactaaaa gtatctggct caagtaattc tacgagtaat agtagccgtc catcaacgaa
      421 ctcaggtggc ggatcatact acacagtaca agcaggcgac tcattatcat taatcgcatc
      481 aaaatatggt acaacttacc aaaacattat gcgacttaat ggtttaaata atttctttat
      541 ttatccaggt caaaaattaa aagtatcagg tactgctagc tcaagtaacg ctgcgagcaa
      601 tagtagccgt ccatcaacga actcaggcgg tggatcatac tacacagtac aagcaggtga
      661 ctcattgtca ttaatcgcat caaaatatgg tacaacttat caaaaaatta tgagcttaaa
      721 tggcttaaat aatttcttta tttatccggg tcaaaaattg aaagtaactg gtaatgcatc
      781 tacgaactca ggatctgcaa caactacgaa tagaggttac aatacaccag tattcagtca
      841 ccaaaactta tatacatggg gtcaatgtac atatcatgta tttaatcgtc gtgctgaaat
      901 tggtaaaggt attagtactt attggtggaa tgctaataac tgggataacg cagcggcagc
      961 agatggttac actatcgaca atagacctac tgtaggttct atcgctcaaa cagatgtagg
     1021 ttattatggt catgttatgt ttgtagaacg tgtaaataac gatggtagta ttttagtttc
     1081 agaaatgaac tattcagctg caccaggtat tttaacttac agaacggtac cagcttacca
     1141 agtaaataat tatagatata ttcactaaag tcttacgtat ataaatatat aatgaattcc
     1201 tattacattt cacaagctga aaagttggat tgaatcctaa actttatggc ggggctatca
     1261 aagtcgtgat tgttgtaata ggaatttttg tataagaaaa aggattggtc gaatgaactt
     1321 catgttctat gttcgaccaa tccttttctg aattagtatt catgctttac tttgcgaatg
     1381 cttgtaaata atctagcacc gtttgttatt aaagtaacaa ctgccactgc ttttttgaac
     1441 ttacgtggtg acttaattga aatgtaaaag tctaacacgg ttcctacagt gcttaaacct
     1501 aatgaaagat atactaattt tttattttta gcatgatatt tatagccatt gtagccgtcg
     1561 actatgaaac ctgcgacatt tagtaaactt gataaacgtt gtgatttgga acgttttgcc
     1621 ataataataa ttccccctag tatcttctgt ttatatgaaa agttgggtgc gctgaattgc
     1681 taacgttttg cgctataact actcatatat gataacataa ttgtacagta taatttgaaa
     1741 aattgatttc acaaagttgg ggtgtcaaag atgattaaat gtgtctgttt agttgaagaa
     1801 acagctgata aaatattgct tgttcaagta aggaatcgcg aaaagtatta tttcccaggt
     1861 ggtaaaatag aagaaggaga atcacaagta cacgcgctgt taagagaagt aaaagaagaa
     1921 ttaaatttaa cattaacaat ggatgaaatt gaatatatcg ggacaattgt aggtcctgca
     1981 tatccacaac aggatatgtt aactgagtta aatggatttc gcgcattaac caaaatcgat
     2041 tgggaaaacg taactatcaa taatgaaatt acggatatac gctggattga taaagataat
     2101 gatgcgttga ttgcgcctgc tgtcaaagtt tggattgaaa cgtatggtgg taaacatgac
     2161 aaataatgac accatcatgt tacgacatta tgtcccacaa gattattcga tgttagaagc
     2221 ttttcaatta agtgaaagtg atttgaagtt tgttaaaacg ccagaggaaa atattacagc
     2281 tgcaatgtct gataatgaaa ggtatcccat cgttgtaatg gatggcaggc aatgtgtggc
     2341 cttttttaca ttacatcgtg gaaaaggggt cgcaccattt agcgataacc aagatgcagt
     2401 atttttcagg tcatttagtg ttgatcaacg ttatcgtaat agaggaatag gtaaagtggt
     2461 aatggaaaaa ttggcgtcat ttatcacttc aacatttcag gatattaatg agattgtgtt
     2521 aacggttaat actgacaatc cacatgccat ggcactttat cgccaacaag gatatcaata
     2581 tatgggagat agtatgttca tcggaagacc tgttcatatt atggcgttaa ctataaaata
     2641 aattaaattt aaaagcatct ttactcatcg tcgaccacaa caattaatga tgaataaagg
     2701 tgctttttgt tatagatcac cggacaattt aatatagtaa aaagcgacct agtgaacaat
     2761 tgacatatat ccacaggtcg cttaacttaa gttatattgc tagttgcgat taattgatag
     2821 actcatcatt tttgcgctgt cgagatggtc tttttattaa aaatgccgta atccaagccg
     2881 taatcggaat actgattgca acggcaatac cgcctaaaat aatagaaata aattcttggg
     2941 caaatatttt cgagtttata atatgaccaa atgaatattt aagtttgaaa aaccaaataa
     3001 ataaagcaag ttggccacca aaaaaggcaa ggtaaatcgt gttcgcagat gtcgctaaaa
     3061 tttctctacc aacacgcatg ccagattgga ataattcgta ttgcgtaagt gttggattta
     3121 cttgatgcaa ttcataaatg ggtgaactaa tggtaatcgt taaatctatc acagctgcaa
     3181 taacagcaag aataatagtg aacaccataa attgaaccat atcaatgcca atattcattg
     3241 aatacacata tgtttcatct tgttgttcgg ttgaaaagcc ttgtagatga ccgaagtaga
     3301 cagataaata aatgagtgta atcaacaata ttgttgtaac gatagtgctg ataaatgcag
     3361 cttgtgtttt aacattgtaa ctattgagta cgaataaatt acaagcgcca ataataatgc
     3421 agaaaaagaa tgtgacgaca taaatcggta cgccaaaaat aatcaataca atactaataa
     3481 ttaaaatagc gaaatttaaa aatagggtta aataagagat gaatcccttt ttacctccga
     3541 aaattatcat cagaaagagg agcaataacg ccaatataaa tacagcattc attgtttcgc
     3601 cctccttaat gtttcaaata tttccataaa caatattgtg ataggaattg taagtacgat
     3661 acctataccg cctgttagtg cgcgcgcaat ttctaacgac caattcatcg aaatagtata
     3721 agtcacagta tttgcatttt ttaaaaagat taaaaacata ggtagtgcac cggataaata
     3781 tgagaataat aagatgttag tcattgttcc cataatatct tggccgatgt ttcgcccagc
     3841 aagcgcccat ctcctcattg aaatgtgtgg cgtacgctgt aaaatttcat gcataccact
     3901 agcaattgta attgcaacat ccataatagc gccaagtgaa cctattaaca ctgaggctag
     3961 gaagatatct ttcggtggta atgataaaaa gttcatcgtt tcatatttaa tgcctttacc
     4021 atctgtcata tatatgatta attctgttaa acctatactc aaaaaagttc cgataattgt
     4081 actggctatg gtaatgagtg tacgcatatg ccagcctgta acgagcaata aagtgagtat
     4141 tgttgaacag atcatggcaa tggtcatgag taagaataaa ttaatattgc tatgttgaat
     4201 atgaatgtaa attgcgatta atatggcaat agaattcaag attaacgata aaatcgattg
     4261 cagtccgact ttgcgaccaa ccaataatac agttaataag aacaaaccag tgatgataac
     4321 cgttaaggta tcacgcttct tttctataat ataagcatca ctcggcttgt tagaaatatg
     4381 taataatact ttttcgtgtg tgcgaaatgc ctcagaatct gcttgcgatt tgacgtactg
     4441 atgattaatc gtcgtcgttt ctccagcaaa ttgaccattt aatattttga cttttaattg
     4501 atttttatat ttaatatcac gattattttg tgcatctttt gtaggtgtcg aagaaacatg
     4561 tttgacatct ataatttgac caattggttt gttgtaaaag ttctcattat tgaatgtaaa
     4621 taaaatagca ccaatgaatg cgatgcagaa caaacctaaa attatattaa atggctttgt
     4681 aaataaattt ctatatttca aaaacaaaac cccaattcta tgaatgaatt aatatggtga
     4741 ttatacgccc ttaatttttt attttcaaag atattactgc taagtgtaaa acgaaaatca
     4801 tcattgatag catcgaatta cttaatggaa tgtagacgtt ttagtcatta attgctgaat
     4861 aagtgttaat aatatgccaa tatcactctt tgtataaggc tcctttgtaa tagcacatat
     4921 cgttcttttt aattcagtat gatctaattt tatatctatc catgatttag attctggtaa
     4981 atgtatattt tgtgatgaaa tgatgtaacc ttctttttga cgaaggagat actgcgcaag
     5041 tggttggcta ctgattgtgt atacatctga tttagtaatc ttgcgcaatt gtttttttac
     5101 agtttcggca aatggtgcca agcaataaat atgactatgc tcaaactgaa ttaatggtgg
     5161 gtgtgtcgcc atcgtaattg gatcgtctga aggcgcatat aaatgatagt gctcttcgaa
     5221 taaaggtagc atatgtaatt gtttgtgttt acgtatttct ggtgtaagtt ccgtgaaacc
     5281 aatgtctata ttcccattta atacgctatt tataatcgtg tcatgatcta ataagctcgg
     5341 tatgacatgt gtatcatttt gtaaatgaaa cgtttggata agtggtagta acatgtggga
     5401 tacgtcactc tcatcatagc caatgtagat acttttattt ttagttaatc catggctttg
     5461 aaattgttca atcgtgctat ctaaatgttc aataatacgc agagcttcat taaataataa
     5521 tttcccttca gatgtgagcg taatattgcg tccttgcttt ttaaataaag acacattaag
     5581 ttcttgttct aataatgtaa tttgacggct tatcgctgat tgagcaatgt ttagttcaag
     5641 tgctgtttcg gagatatgtt ctcttttagc gacctcgata aaatatctta attgtttaat
     5701 ttccatagcg atataggcac ctccgaaaat gagtgttttg taactattat agcaatatta
     5761 ttgataaatg ttctattttt tagatgaata tcttctattt tatatattga acagataaat
     5821 tttttagatt atagtaatta tcattaataa ctaatatcag aatattctaa aaaaggggtg
     5881 tgcatcatgc acaatgagaa attaattaaa ggcttatatg actatcgtga ggaacatgat
     5941 gcgtgtggta ttggttttta tgcgaatatg gataataaaa ggtctcacga catcattgat
     6001 aaatcgcttg aaatgttgcg acgcttagat cacaggggcg gggtcggcgc agatggcatc
     6061 actggtgatg gcgcaggtat tatgactgaa ataccttttg catttttcaa acaacatgta
     6121 acggactttg atatcccagg tgaaggtgaa tatgccgtgg ggttattttt ttccaaagaa
     6181 cgcattttag gttctgaaca tgaagcagtt tttaaaaaat attttgaagg cgaagggtta
     6241 tcaattcttg gttatcgtaa tgtaccagtt aataaagatg ccattgctaa acatgtagca
     6301 gatacgatgc cagtcattca acaagtgttt attgatatta ggggcattga agatgttgaa
     6361 aagcgtttgt ttttagcgag aaaacaatta gagttctatt cgactcagtg cgatttagaa
     6421 ttgtatttta cgagcttatc acgcaaaaca attgtatata aaggttggtt acgatcagac
     6481 caaattaaaa aactatatac agatttatcg gatgatttat atcaatcaaa gctagggtta
     6541 gtgcattcga gatttagtac gaatacattc ccgagttgga aaagggcaca tcctaaccgt
     6601 atgttaatgc ataatggtga gattaacacg attaaaggta atgtaaactg gatgcgagca
     6661 cgccaacata aattaatcga aacattattt ggcgaggatc aacataaagt gtttcaaatt
     6721 gtcgatgagg atggtagtga ctctgctatt gtagataatg cgctagagtt cttatcgtta
     6781 gccatggagc cagaaaaggc agcgatgcta ctcatacctg aaccttggtt atacaatgaa
     6841 gcgaatgatg caaatgtacg tgcgttttat gaattttata gttatttaat ggaaccgtgg
     6901 gatggtccta caatgatttc gttctgtaac ggtgacaaac ttggcgcgct tacagataga
     6961 aatggattac gtccaggtcg ttatacgatt actaaagata actttattgt cttttcatct
     7021 gaagtgggtg ttgtggacgt acctgaaagt aatgttgctt ttaaaggtca attgaatcct
     7081 ggaaagttat tgcttgttga ttttaaacag aataaagtca ttgaaaataa tgatttaaaa
     7141 ggtgcgattg ctggagaatt accatataaa gcgtggattg ataaccataa agttgacttt
     7201 gattttgaaa atatacaata tcaagattcg caatggaaag atgagacgtt atttaaatta
     7261 caacgtcagt ttgcatacac gaaagaagag attcataagt atattcagga acttgtagaa
     7321 ggtaagaagg atcctatcgg tgcaatggga tatgatgcgc caattgcagt gttgaacgag
     7381 cgaccagaat cactatttaa ttactttaaa cagctgtttg cacaagttac gaatccacca
     7441 attgatgcgt atcgtgaaaa aatcgtaacg agtgaacttt cttatttagg tggcgaaggt
     7501 aacttactag cacctgacga aacggtttta gatcgtattc aattgaaaag gccggtattg
     7561 aatgaatcac acttagcagc gattgatcag gaacatttta aattaactta tttatcaacg
     7621 gtatatgaag gggatttgga agatgcgtta gaagcattag gccgagaagc agtgaatgct
     7681 gtaaagcaag gcgctcaaat tctagtgtta gatgacagtg gattagttga tagcaatggc
     7741 tttgcaatgc cgatgttact cgcaataagt catgtgcatc aattacttat taaagcagat
     7801 ttacgtatgt ctacaagttt agtcgctaaa tctggtgaga cacgagaagt gcatcatgtt
     7861 gcttgtttac tcgcatatgg cgcgaatgca attgtgccat acctagcgca acgtacagtt
     7921 gaacaactga cattgacaga agggttacaa ggcaccgttg tcgataatgt taagacatat
     7981 acggatgtat tgtcagaagg tgtcattaaa gtaatggcta agatgggaat ttcgacagtg
     8041 caaagttatc aaggtgcaca aatatttgaa gcgattggct tgtctcatga tgtgattgat
     8101 cgttatttta ctgggacaca gtctaagtta tcgggtattt cgattgatca aattgatgct
     8161 gaaaataaag cacgtcaaca aagtgatgat aattatcttg catcaggtag tacatttcaa
     8221 tggagacaac aaggtcaaca tcatgctttt aatccggaat ctattttctt attgcagcac
     8281 gcatgtaaag aaaatgacta tgcgcaattt aaagcatact ctgaagcggt gaacaaaaat
     8341 agaacagatc acattagaca tttacttgaa tttaaagcat gtacaccgat tgacatcgac
     8401 caagttgaac cggtaagtga cattgtcaaa cgctttaata caggggcgat gagttatgga
     8461 tcgatttcag cggaagcaca tgaaacgtta gcacaagcca tgaaccaatt aggtggaaag
     8521 agtaatagtg gtgaaggtgg cgaagatgca aaacgttatg aagtacaagt tgatggaagc
     8581 aacaaagtaa gtgcgattaa acaagttgct tctgggcgtt ttggtgtaac tagtgattat
     8641 ttacaacatg ccaaagaaat tcaaattaaa gttgcgcaag gtgcaaagcc tggtgaaggt
     8701 ggtcaattac ctggtactaa ggtatatccg tggattgcaa agacaagagg gtcaacgcca
     8761 ggtatcggtc tgatttcacc accgccacat catgatattt attcaataga agatttagcg
     8821 caactgatac atgatttgaa aaatgcgaat aaagatgcag atatcgcggt aaaattagtt
     8881 tcgaaaacag gtgttggtac cattgcatct ggggtggcaa aagcatttgc agataaaatt
     8941 gtcatcagtg gttacgatgg tggtacaggg gcttcaccta aaacgagtat tcagcatgcc
     9001 ggtgttcctt gggagattgg tttagcagaa acacatcaaa cattaaaact aaatgactta
     9061 agaagtcgtg ttaagttaga aacagacggt aagttattaa ctggtaaaga tgtagcgtac
     9121 gcatgtgcgc ttggagcgga agaatttgga tttgcaactg caccattagt ggtgttgggc
     9181 tgtattatga tgcgtgtatg ccataaagat acatgtccag taggagttgc aactcaaaac
     9241 aaagatttac gtgctttata tagaggtaaa gcacatcatg ttgttaattt tatgcatttt
     9301 attgcacaag aattaagaga aattttagca tctttaggtt tgaaacgtgt agaagactta
     9361 gttggaagaa ctgatttatt acaacgatca tcaacattaa aagcgaatag caaagcggct
     9421 agtattgatg ttgaaaaact gttatgtcct ttcgatgggc caaacacaaa agaaattcaa
     9481 caaaatcata atcttgagca tggatttgat ttaacaaatt tatatgaagt aacgaagcca
     9541 tatattgctg aaggacgtcg ctatacaggt agctttacag taaataatga acaacgtgat
     9601 gtaggagtta ttacaggtag tgagatttcg aaacaatatg gagaagcagg acttcctgaa
     9661 aatacaatta atgtttatac gaatggtcat gctggtcaaa gtcttgcagc atatgcaccg
     9721 aaaggcttaa tgattcatca tactggagat gcgaatgact atgttggtaa aggattatct
     9781 ggtggtacgg tcattgtcaa agcacctttt gaagaacgac aaaatgaaat tattgctggt
     9841 aacgtctcat tctatggtgc gacaggtggt aaggcattta ttaacggtag tgcaggagaa
     9901 agattctgta ttagaaatag tggtgtagat gttgtcgttg aaggtatcgg tgaccatgga
     9961 ttagagtaca tgactggtgg acatgtcatt aatttaggtg atgtaggtaa gaacttcggt
    10021 caaggtatga gtggtggtat tgcttacgtt atcccgtctg atgtagaagc ttttgttgaa
    10081 aataatcaac tagatacgct ttcgtttaca aagattaaac accaagaaga aaaagcattc
    10141 attaagcaaa tgctggaaga acatgtgtca cacacgaata gtacgagagc gattcatgtg
    10201 ttaaaacatt ttgatcgcat tgaagatgtc gtcgttaaag ttattcctaa agattatcaa
    10261 ttaatgatgc aaaaaattca tttgcacaaa tcattacatg acaatgaaga tgaagcgatg
    10321 ttagctgcat tttacgatga cagtaaaaca atcgacgcta aacataaacc agccgttgtg
    10381 tattaaggaa agggggagat acgatgggtg aatttaaagg atttatgaag tatgacaaac
    10441 agtacttagg tgaattatca ctggtagacc gtttgaagca tcataaagca tatcaacaac
    10501 gatttactaa agaagatgcc tctatccaag gtgcgcgatg tatggattgt ggaacgccgt
    10561 tttgtcaaac cggacaacag tatggtaggg aaacaatagg ttgtccaatt ggaaactaca
    10621 ttcctgaatg gaacgactta gtgtatcatc aagattttaa aactgcttat gaacgcttaa
    10681 gcgaaacaaa taactttcct gactttacag ggcgtgtatg tcctgcacca tgcgaaagtg
    10741 cttgtgtgat gaagattaat agagaatcga ttgcgattaa aggtattgaa cgcacaatta
    10801 ttgatgaagc ttttgaaaat ggttgggtag cgccgaaagt tccgagtcgc cgtagagatg
    10861 aaaaagtggc aatcgttgga agcggtccag caggattaac tgctgctgaa gaacttaatc
    10921 tactaggata tcaagtgact atttatgaac gtgctagaga atcaggcggt ttattaatgt
    10981 atggtattcc aaatatgaag cttgataaag atgtggttcg acgtcgtatt aagttaatgg
    11041 aagaagcggg cattactttc attaatggtg ttgaagtagg cgttgatatt gataaagcaa
    11101 cgttagaatc tgagtatgat gccattatat tatgtactgg tgcacaaaaa ggtagagatt
    11161 tacctttaga aggacgcatg ggtgatggta tacatttcgc tatggattat ttaactgaac
    11221 aaacgcagct gttaaatgga gaaattgatg atattacaat aactgcaaaa gataagaatg
    11281 tcattatcat tggtgctggt gatacagggg cagactgtgt agcgacagca ttaagagaaa
    11341 attgtaaatc gattgttcaa tttaataaat atacgaaatt gccagaagca attacattta
    11401 cagaaaatgc atcatggcct ttagcaatgc cggtgtttaa aatggactat gcgcaccaag
    11461 agtacgaagc taagtttggt aaggaaccac gtgcatatgg tgttcaaaca atgcgttacg
    11521 atgttgacga taaaggacac atacgtggtt tgtatactca aattttagag caaggcgaaa
    11581 atggtatggt catgaaagaa ggacctgaaa gattttggcc tgctgacctt gtattattat
    11641 caatcggctt cgaaggtaca gaaccaacag taccgaatgc ttttaacatt aaaacggata
    11701 gaaatcgaat cgtggcggat gatacaaact atcaaactaa taatgaaaag gtatttgctg
    11761 ctggagatgc tagacgtggt caaagtttag ttgtatgggc aattaaagaa ggtagaggcg
    11821 tagcgaaagc agtagatcag tatttagcga gtaaagtttg tgtataatct ttgtatggaa
    11881 atggtggtta cgttgacgtt gtgtcatgct gaatcgagtt tgaaataatc tagtatctat
    11941 caacgtcaca tgccatcttt gtaacctaaa aacaaaggtt tgtaagacaa caaatcgatt
    12001 aattataagt agtgattttt tacattcgtt tataggtcaa ctgtagtgga agacaatgat
    12061 ttgtggtaat catgtaatgc ttaaaaacaa tattgacttt tacagaacgt tcatatatga
    12121 taaatattgt gtttaggagg aatacccaag tccggctgaa gggatcggtc ttgaaaaccg
    12181 acaggggctt aacggctcgc gggggttcga atccctcttc ctccgccatc aatatttata
    12241 ttaaatacta tatataatga aggtaagtgc tcaaattttg agtatttgcc ttttttattt
    12301 gtctttgaat ggctcgtttt tttagctgta gaaatgatag ggcattgaga ttggaagggc
    12361 atttggcttg tgcaatatac atagctaaat gtcttttttg ttttgtgaaa tatgatggat
    12421 ggcttgtgtg ggcaagtttg ctatttatag atatgcattt ttcaatttag gagttggcca
    12481 tgcatctaca ctttataatg gtgagagcgt ggtgaggtat tgttaatcac gcaattgtag
    12541 cgaggagtta ttgctacata tgtcgttatg gcctattgat tttctaaaat agctgtatca
    12601 gatcatgtga caaaataaaa ataatttgtt gaaagccttt acataacttg tctagacaag
    12661 ttatactcgt tttaagacat taagggagtg aaatatatgg ctgtaaaaag agaagatgta
    12721 aaagccatcg taagcgctat tgggggaaaa gaaaatcttg aagctgcaac gcattgtgta
    12781 acacgattac gtttagtgct taaagatgaa agcaaagttg ataaagacgc attaagtaat
    12841 aacgcgttgg tcaaggggca gttcaaagca gaccatcaat atcaaattgt cattggtcca
    12901 ggaacagtcg atgaagtgta taagcagttt attgatgaaa caggtgctca agaagcttcg
    12961 aaagatgaag cgaaacaagc agctgcgaaa aaagggaatc cagtacaacg tttgatcaaa
    13021 ttgttagggg atatttttat accaatatta cctgcgattg tgacagctgg tttgttaatg
    13081 gggatcaata atttacttac aatgaaaggt ttatttggtc caaaagcact tattgagatg
    13141 tatccgcaaa ttgctgatat ttcaaacatc attaatgtga ttgcgagtac ggcatttatt
    13201 ttcttaccag cattaattgg ttggagtagt atgcgtgtat ttggtggtag tccgattcta
    13261 ggcatagtct taggtttgat tttaatgcat ccgcaattag tatctcagta tgatttggca
    13321 aaagggaata ttccgacgtg gaacttattt ggcttagaga ttaagcagtt gaattaccaa
    13381 ggtcaagtgt tgcctgtttt aattgcagct tatgttctag ctaaaattga aaaaggatta
    13441 aataaagtcg ttcacgattc gataaaaatg ttggtcgttg gacccgtagc gcttttagtt
    13501 actggatttt tagcatttat tatcattgga ccagttgcat tattgattgg tacaggtatt
    13561 acatctggtg ttacatttat attccaacat gcaggatggc ttggcggagc aatatatgga
    13621 ttgttatatg caccacttgt aattacagga ctacaccata tgtttttagc agtagatttc
    13681 caattgatgg gtagcagctt aggtggtacg tatttatggc caattgttgc gatttccaat
    13741 atttgtcagg gctctgcagc atttggagca tggtttgtct ataaacgtcg taaaatggtt
    13801 aaagaagaag gcttggcatt aacatcttgt atttctggta tgttaggtgt tactgaacca
    13861 gctatgtttg gtgtgaactt acctttgaaa tatccattta tcgctgcgat atcaacgtct
    13921 tgtgtattgg gggcaatcgt tggtatgaat aacgtacttg gaaaagttgg tgttggtggc
    13981 gtgccagcat tcatttcaat tcaaaaagaa ttttggccag tatatcttat tgtgacagct
    14041 attgctattg ttgtaccatg tatactaaca attgtgatgt ctcattttag taaacaaaaa
    14101 gcgaaagaaa ttgttgaaga ttaataaaat aaaaaagggg cgttcgttat ttggacgtcc
    14161 tttattacgt tataaggtgg taattgtgtg tcgaaagaaa tagattggag aaaatccgtt
    14221 gtatatcaaa tttatcctaa gtcgtttaat gatacgacgg ggaatggtat aggagatatc
    14281 aacggaatta tagaaaaatt ggattatatc aagttattgg gtgttgatta tatttggtta
    14341 acaccagtgt atgaatcacc tatgaatgat aatggctatg atatcagcaa ttatttagaa
    14401 atcaatgaag cctttggaac gatggatgat tttgaaaagt taatcaaagt tgctcatcaa
    14461 aaagacttga aagtgatgtt agatattgtt attaatcata cgtcgacgga gcatgaatgg
    14521 tttaaagaag cccgtaaatc taaagataac ccttatagag attattactt tttcagatca
    14581 tctgaagacg ggccgccaac aaattggcat tctaaattcg gtggtaatgc atggaagtat
    14641 gattctgaga cagatgaata ttatttacat ttatttgatg tcagtcaagc tgatttaaat
    14701 tgggataatc cggaagtacg tcaatcgtta tatcgcatag tcaatcattg gatagacttc
    14761 ggcgttgatg gttttcgatt tgatgtcatt aacttaattt ctaaaggtga atttaaggac
    14821 tctgacaaaa taggtaaaga attttatacg gatggtccta gagtgcatga gtttctgcat
    14881 gaattaaatc gtcaaacgtt tggtaacact gacatgatga ctgtaggaga aatgtcttcg
    14941 acgacgattg aaaattgtat taagtataca caaccagaac gccaagaatt gaatagtgtt
    15001 tttaattttc atcatctaaa ggttgattat gttgatggtg aaaagtggac aaatgcgaag
    15061 cttgattttc ataagttaaa ggaaattctg atgcaatggc aacgaggtat ttatgacggt
    15121 ggcggatgga acgcgatttt ctggtgtaat catgatcagc cacgggtagt gtctagattt
    15181 ggtgatgata cgtcggaaga gatgaggata caaagtgcta aaatgttagc tatcgcactg
    15241 catatgttgc aagggacgcc atatatttac caaggtgaag aaattggtat gacggaccca
    15301 cattttacat caatagcaca atatcgtgat gttgaatcga ttaatgccta ccatcagttg
    15361 ctaagtgaag ggcatgctga agcggatgta ttagcgattt taggacagaa gtcacgagac
    15421 aattcgagaa cgcctatgca atggagtgat gatgttaatg ctggatttac agctggtaag
    15481 ccttggattg atatttcgga aaattatcat caggtcaacg ttagacaagc acttcagaat
    15541 aaagagtcta ttttctatac gtatcaaaaa ttaatacaat taagacatac gcatgatatt
    15601 attacgtatg gagacattgt gccacgtttt atggatcatg atcatttatt tgtttatgaa
    15661 cgtcattata agaatcaaca atggctagta attgcgaatt tctcagcatc ggctgttgat
    15721 ttgccagaag gattggctag agaaggttgt gttgtgattc aaacaggcac agtggaaaat
    15781 aatacgataa gcgggtttgg tgcaattgta atcgaaacaa acgcgtaaaa taaattgagt
    15841 ggatgcgttt atatggcgaa acaaaaaaag tttatgaaga tttatgaggc gttgaaagaa
    15901 gatatattaa acgggcagat tcaatatggt gaacaaattc cgtctgaaca tgatttggtg
    15961 caattgtacc agtcatctcg agagaccgtg cgtaaggcat tagatttgtt ggcattagac
    16021 ggcatgattc aaaagattca tggtaaaggg tcacttgtca tttatcagga ggttacagag
    16081 tttccatttt ctgaacttgt tagttttaaa gaaatgcaag aagaaatggg cgtcgcatat
    16141 ttaactgaag ttgttgtgaa tgaggttgtt gaagcgcatg aagttccaga agttcaacat
    16201 gctttaaaca tcaattctag tgaatcgctc attcatattg ttagaactcg tcggcttaac
    16261 caacatgtga agattgttga tgaagattat tttctaaagt cgattgtttc agatataggt
    16321 aatgatgttg cgagtgattc tatttatgat tatttggaaa aggtattaaa tcttaatatt
    16381 agttattcaa gtaagtctat tacttttgaa ccgtttgatg aacaagcata tcaattgttt
    16441 ggtgatgtat cggtggctta ttcagcaaca gttcgaagta ttgtgtattt agaaaataca
    16501 atgccgtttc aatataatat ttcaaaacat cttgcaaatg aatttaaatt taacgacttc
    16561 tcaagacgtc gtataaagta aacaatgata taaatgattt atacttgcaa ttaactatta
    16621 aaatatagta atatatatct tgccgtgcta ggtggggagg tagcggttcc ctgtactcga
    16681 aatccgcttt atgcgaggct taattccttt gttgaggccg tatttttgcg aagtctgccc
    16741 aaagcacgta gtgtttgaag atttcggtcc tatgcaatat gaacccatga accatgtcag
    16801 gtcctgacgg aagcagcatt aagtggatca tcatatgtgc cgtagggtag ccgagattta
    16861 gctaacgact ttggttacgt tcgtgaatta cgttcgatgc ttaggtgcac ggttttttat
    16921 tttttaaata ttaaaccgat tattaagagt tgaaaatata tatttattta tagaagctac
    16981 tttcttgaag acaattcagc gtattatacg tggaacatgt ttgtgggaag tagctttttt
    17041 atatgtgaag tttgattcaa gtgaactcga tgtgcagttt gaatgatttt tgtgtcaatg
    17101 aaaagtaaga agttataatt tgatgataaa gaaatgatgg tgaaatgagg gggagtatct
    17161 tacaatagaa ttattaatga gatacgttat gattattgac aatcaaatgc ctacggagga
    17221 catatgcaaa tatatttaag tactttaaca gagttagatt atgataaatc tttaaatagt
    17281 attgaagaaa gttttgatga taatcctgaa acgagttggc aagcacgtgc gaaagtaaaa
    17341 catttaagaa aatctccttg ctataatttt gaattagaag taatagcgaa aaatgaaaat
    17401 aacgatgtcg ttggacacgt tttattaatt gaagtagaaa ttaatagtga tgataagacg
    17461 tattatggtt tggcgattgc ctctttatca gttcatcctg aattacgtgg acaaaaatta
    17521 ggtcgtggct tggttcaagc agtagaagag cgtgccaaag cacaagagta tagtacggtt
    17581 gttgtagacc attgttttga ctactttgaa aagttgggtt atcaaaatgc tgctgagcat
    17641 gacattaaat tagaatctgg tgatgcaccg ttacttgtaa aatatttatg ggataatttg
    17701 acggatgcac cacacggaat cgtaaaattt ccagaacatt tttattaatt gttcaattaa
    17761 gaagtaaagg tattatcatg ctataatgag aggtaattgt ttatggaggt gctaacttga
    17821 attatcaagc cttatatcgt atgtacagac cccaaagttt cgaggatgtc gtcggacaag
    17881 aacatgtcac gaagacattg cgcaatgcga tttcgaaaga aaaacagtcg catgcttata
    17941 tttttagtgg tccgagaggt acggggaaaa cgagtattgc caaagtgttt gctaaagcaa
    18001 tcaactgtct aaatagcact gatggagaac cttgtaatga atgtcatatt tgtaaaggca
    18061 ttacgcaggg gactaattca gatgtgatag aaattgatgc tgctagtaat aatggcgttg
    18121 atgaaataag aaatattaga gacaaagtta aatatgcacc aagtgaatcg aaatataaag
    18181 tttatattat agatgaggtg cacatgctaa caacaggtgc ttttaatgcc cttttaaaga
    18241 cgttagaaga acctccagca cacgctattt ttatattggc aacgacagaa ccacataaaa
    18301 tccctccaac aatcatttct agggcacaac gttttgattt taaagcaatt agcctagatc
    18361 aaattgttga acgtttaaaa tttgtagcag atgcacaaca aattgaatgt gaagatgaag
    18421 ccttggcatt tatcgctaaa gcgtctgaag ggggtatgcg tgatgcatta agtattatgg
    18481 atcaggctat tgcatttggt gatggtacgt taacattgca agatgcgttg aatgtcacag
    18541 gtagcgtaca tgatgaagcg ttggatcact tgtttgatga tattgtacaa ggtgacgtac
    18601 aagcatcttt taaaaaatac catcagttta taacagaggg taaagaagtg aatcgcctaa
    18661 taaatgatat gatttatttt gtcagagata cgattatgaa taaaacatct gagaaagata
    18721 ctgagtatcg agcactgatg aacttagaat tagatatgtt atatcaaatg attgatctta
    18781 ttaatgatac attagtgtcg attcgtttta gtgtgaatca aaacgttcat tttgaagtgt
    18841 tgttagtaaa attagctgag cagattaagg gtcaaccaca agtgattgcg aatgtagctg
    18901 aaccagcaca aattgattca tcgccaaaca cagatgtatt gttgcaacgt atggaacagt
    18961 tagagcaaga attaaaaaca ctaaaagcac aaggagtgag tgtcgctcct gctcaaaaat
    19021 cttcgaaaaa gcctgcgaga ggcatacaaa aatctaaaaa tgcattttca atgcaacaaa
    19081 ttgcaaaagt gctagataaa gcgaataagg cagatatcaa attgttgaaa gatcattggc
    19141 aagaagtgat tgatcatgcc aaaaacaatg ataaaaaatc actcgttagt ttattgcaaa
    19201 attcggaacc tgtggcggca agtgaagatc acgtacttgt gaaatttgag gaagagatcc
    19261 attgtgaaat cgtcaataaa gacgacgaga aacgtagtag tatagaaagt gttgtatgta
    19321 atatcgttaa taaaaacgtt aaagttgttg gtgtaccatc agatcaatgg caaagagttc
    19381 gaacggagta tttacaaaat cgtaaaaacg aaggcgatga tatgccaaag caacaagcac
    19441 aacaaacaga tattgctcaa aaagcaaaag atcttttcgg tgaagaaact gtacatgtga
    19501 tagatgaaga gtgatacatg acaagcgata taatcgtatg tataatgaaa gaaacatcat
    19561 tttattgata aatatttatt gattttcaag gaggaaatgg aatatgcgcg gtggcggaaa
    19621 catgcaacaa atgatgaaac aaatgcaaaa aatgcaaaag aaaatggctc aagaacaaga
    19681 aaaacttaaa gaagagcgta ttgtaggaac agctggcggt ggcatggttg cagttactgt
    19741 aactggtcat aaagaagttg tcgacgttga aatcaaagaa gaagctgtag acccagacga
    19801 tattgaaatg ctacaagact tagtgttagc agctactaat gaagcgatga ataaagctga
    19861 tgagcttact caagaacgtt taggtaaaca tactcaaggc ttaaacatcc ctggaatgtg
    19921 atcatagatg cattatccag aacctatatc aaagcttatt gatagcttta tgaaattgcc
    19981 aggcattggt ccaaagacag cccaacgtct ggcttttcat accttagata tgaaagaaga
    20041 cgatgttgtt cagtttgcca aagcattagt agatgttaag agagaattaa catattgtag
    20101 cgtatgtggt cacattactg aaaatgatcc atgttatatt tgtgaagata aacaaagaga
    20161 tcgttcagtt atttgtgttg tggaagatga caaagatgtc atagctatgg aaaaaatgag
    20221 agaatacaaa ggtttatatc acgttttaca tgggtctatt tcacctatgg atggcattgg
    20281 accagaagat attaatattc cttcattgat tgaacgcttg aaaagcgatg aagttagcga
    20341 attaatctta gctatgaacc cgaacttaga gggggaatct acagccatgt atatttctag
    20401 attagttaag cctataggta tcaaagtgac gagattagca caaggattat cggtaggtgg
    20461 cgatttagag tatgctgacg aagtaacatt atctaaagca atcgcaggta gaacagaaat
    20521 gtaatgtctt ctattaaaca tttttgaatt taatactata gtaagaaaag tcacagtgta
    20581 atcattgtgg ctttttttat ggtgtggtgt gatgtactac tttatttgcg gtgtggcggt
    20641 ggtatggttt acctagtttt actgagggat gggtaatctt taggaagcaa gccgttggtt
    20701 gtgatttgtt acttctaata gtaatgatgt gaattggatt atcgaattag atttacggtt
    20761 atggtgtgtt ggtgctatta atttgataaa tgcggttgat ggttaatgac tatgcaaatg
    20821 aaattctttt gtaattgaaa tgatagatgc tggcttagta gttgtacttc tttggtctaa
    20881 agcttattaa atcagcctgt atagcggtgt tttgagagat tatttaaaac ttgtaaattt
    20941 atttttaatt tctggtaaaa aaataacgtt ctgttttgcg gtttttttga ttgatatggt
    21001 tagagaaaaa tctgtttctt gttctaaaaa acgtactatt tataagtggg gattttttaa
    21061 gttcgatttt taggataagg gcgttcagta cagatgacaa aggtgtaatt tttactgttg
    21121 ttaagcagtt tgaaagcctg tatagtattt atttgttgag gcaaacaaaa caactcaact
    21181 taagaaataa cttgaattac taacgaaaat taattttaaa aagttgttga cttaaatgtt
    21241 aataaaatgt ataattaatt cttgtcggta ag
//
LOCUS       DAOBBH010000027        21181 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.027, whole genome shotgun sequence.
ACCESSION   DAOBBH010000027 DAOBBH010000000
VERSION     DAOBBH010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21181
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.027"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(193..1452)
                     /gene="femB"
                     /locus_tag="P6L01_000740"
     CDS             complement(193..1452)
                     /gene="femB"
                     /locus_tag="P6L01_000740"
                     /EC_number="2.3.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemB"
                     /protein_id="HDP3029825.1"
                     /translation="MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLL
                     GIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQC
                     LYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
                     VLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGF
                     VSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDK
                     QMKKIAELDKQIDHDQHELLNASELSKTDGPILNLASGVYFANAYEVNYFSGGSSEKY
                     NQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELI
                     GDFYKPIHKVKYWLFTTLDKLRKKLKK"
     gene            complement(1471..2733)
                     /gene="femA"
                     /locus_tag="P6L01_000741"
     CDS             complement(1471..2733)
                     /gene="femA"
                     /locus_tag="P6L01_000741"
                     /EC_number="2.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemA"
                     /protein_id="HDP3029826.1"
                     /translation="MKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVG
                     IKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRC
                     LYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSV
                     LDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRYLSEEELPIFRSFMEDTSESKAFA
                     DRDDKFYYNRLKYYKDRVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPEN
                     KKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNELPISAGFFFINPFEVVYYAGGTSN
                     AFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIE
                     YVGDFIKPINKPVYAAYTALKKVKDRIF"
     gene            complement(3055..3783)
                     /locus_tag="P6L01_000742"
     CDS             complement(3055..3783)
                     /locus_tag="P6L01_000742"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDP3029827.1"
                     /translation="MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGP
                     VIMEAGQQAIKQGITIDYIFNQLEKHGDQIKCNYVLMTYYNIICHYGEQAFFEKCRDT
                     GVYGLIIPDLPYELSQRLKQQFSHYGVKIISLVAMTTDDKRIKDIVSHAEGFIYTVTM
                     NATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIV
                     KRFKSNTREEIIRYLQSIQQTLNN"
     gene            complement(3776..4990)
                     /gene="trpB"
                     /locus_tag="P6L01_000743"
     CDS             complement(3776..4990)
                     /gene="trpB"
                     /locus_tag="P6L01_000743"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDP3029828.1"
                     /translation="MNKQIQTEADELGFFGEYGGQYVPETLMPAIIELKKAYKEAKAD
                     PEFQRELEYYLSEYVGRATPLTYAASYTESLGGAKIYLKREDLNHTGAHKINNALGQA
                     LLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRMELLG
                     AKVVAVEEGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQSVIGKE
                     IKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGEDTDKHALA
                     IGKGSPGVLHGTKMYLIQDEGGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENAS
                     DTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETI
                     RQYMAERGLAND"
     gene            complement(4983..5615)
                     /locus_tag="P6L01_000744"
     CDS             complement(4983..5615)
                     /locus_tag="P6L01_000744"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HDP3029829.1"
                     /translation="MKLKFCGFTSIKDVTAASQLPIDAIGFIHYEKSKRHQTITQIKK
                     LASAVPNHIDKVCVMVNPDLTTIEHVLSNTSINTIQLHGTESIDFIQEIKKKYSSIKI
                     TKALAADENIIQNINKYKGFVDLFIIDTPSVSYGGTGQTYDWTILKHIKDIPYLIAGG
                     INSENIQTVNQLKLSHQGYDLASGIEVNGRKDIEKMTAIVNIVKGDRDNE"
     gene            complement(5615..6397)
                     /gene="trpC"
                     /locus_tag="P6L01_000745"
     CDS             complement(5615..6397)
                     /gene="trpC"
                     /locus_tag="P6L01_000745"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HDP3029830.1"
                     /translation="MTILAEIVKYKQSLLQNGYYQDKLNTLKSVKIQNKKSFINAIEK
                     EPKLAIIAEIKSKSPTVNDLPERDLSQQISDYEKYGANAVSILTDEKYFGGSFERLQA
                     LTTKTTLPVLCKDFIIDPLQIDVAKQAGASMILLIVNILSDKQLKDLYNYAISQNLEV
                     LIEVHDRHELERAYKVNAKLIGVNNRDLKRFVTNVEHTNTILENKKPNHHYISESGIH
                     DASDVRKILHSGIDGLLIGEALMRCDNLSEFLPQLKMQKVKS"
     gene            complement(6399..7397)
                     /gene="trpD"
                     /locus_tag="P6L01_000746"
     CDS             complement(6399..7397)
                     /gene="trpD"
                     /locus_tag="P6L01_000746"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HDP3029831.1"
                     /translation="MTLLTRIKTETILLESDIKELIDILISPSIGTDIKYELLSSYSE
                     REIQQQELTYIVRSLINTMYPHQPCYEGAMCVCGTGGDKSNSFNISTTVAFVVASAGV
                     KVIKHGNKSITSNSGSTDLLNQMNIQTTTVDDTPNQLNEKDLVFIGATESYPIMKYMQ
                     PVRKMIGKPTILNLVGPLINPYHLTYQMVGVFDPTKLKLVAKTIKDLGRKRAIVLHGA
                     NGMDEATLSGDNLIYELTEDGEIKNYTLNATDYGLKHAPNSDFKGGSPEENLAISLNI
                     LNGKDQSSRRDVVLLNAGLSLYVAEKVDTIAEGIELATTLIDNGEALKKYHQMRGE"
     gene            complement(7403..7969)
                     /locus_tag="P6L01_000747"
     CDS             complement(7403..7969)
                     /locus_tag="P6L01_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDP3029832.1"
                     /translation="MILVVDNYDSFTYNLVDIVAQHTDVIVQYPDDDNVLNQSVDAVI
                     ISPGPGHPLDDQQLMKIISTYQHKPILGICLGAQALTCYYGGEVIKGDKVMHGKVDTL
                     KVISHHQHLLYQDIPEQFSIMRYHSLISNPDNFPEELKITGRTEDCIQSFEHKERPHY
                     GIQYHPESFATDYGVKIITNFINLVKEG"
     gene            complement(7966..9372)
                     /locus_tag="P6L01_000748"
     CDS             complement(7966..9372)
                     /locus_tag="P6L01_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDP3029833.1"
                     /translation="MDIFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSVVIF
                     DIYGTLTLDNDVLSVSTLKESYQIIERPYHYLTTKINEDYHNIQDEQLKSLPFISGYV
                     GTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNST
                     KSDLENRVNKSIEDLTKIQPFMPTQDFDFKTKEIQSNISEERFIEMIQYFKEKITEGD
                     MFQVVPSRIYKYAHHASQHLNQLSFQLYQNLKRQNPSPYMYYLNIDQPYIVGSSPESF
                     VSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKECSEHRMLVDLGRNDIHRVS
                     KIGTSKITKLMVIEKYEHVMHIVSEITGKINQNLSPMTVIANLLPTGTVSGAPKLRAI
                     ERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMIDEQYINVEAGCGVVYDSIPE
                     KELNETKLKAKSLLEVRP"
     gene            complement(9864..10895)
                     /locus_tag="P6L01_000749"
     CDS             complement(9864..10895)
                     /locus_tag="P6L01_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDP3029834.1"
                     /translation="MEPILEMIKTLTGINSPSGDTEEAIQFVEKYAKDLGYQTTLTNK
                     GALLITVPGKNDEVQRCITAHVDTLGAMVKEIKEDGRLAIELIGGFTYNAIEGEYCQI
                     KTDAGQIYTGTICLHETSVHVYRNNHEIPRDQKHMEIRIDEVTTSEEDTKSLGISVGD
                     FVSFDPRTVITSSGFIKSRHLDDKASVAMILQLLKKLKEEQIILPHTTQFYISNNEEI
                     GYGANASIDSKIKEYIALDMGALGDGQASDEYTVSICAKDASGPYHKQLKSHLVNLCK
                     INNIPYKVDIYPYYGSDASAALHAGADIRHGLFGAGIESSHAMERTHIDSIKATEKLL
                     YAYCLSPIE"
     gene            11060..12151
                     /locus_tag="P6L01_000750"
     CDS             11060..12151
                     /locus_tag="P6L01_000750"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDP3029835.1"
                     /translation="MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADTSQLDKAKS
                     IGIINEKCLNYSEAIKKADVIIYATPVAITNKYLSELIDMPTKPGVIVSDTGSTKAMI
                     QQHESNLLKHNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEPRNEQAANTLK
                     ELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNGQEHHLVNKLAAGGF
                     RDITRIASSNAQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLSFFAQA
                     KSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYIMSLHNISISNLRILEVREDI
                     YGALKISFKNPTDRERGMQALSDFDCYIQ"
     gene            complement(12288..13550)
                     /locus_tag="P6L01_000751"
     CDS             complement(12288..13550)
                     /locus_tag="P6L01_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDP3029836.1"
                     /translation="MYNYHLLEDRDVLCIDQKSFFASVSCIEKGLDPLETKLAVVADT
                     KRQGSVILAATPKLKELGIKTGSRLFEIPHRNDIYIINPSMRKYLNVSVAISKIALRY
                     IPPEDLHQYSIDEFFMDVTDSYHRFSSTVHAFCERLKREIYEETGIYCTVGIGSNMLL
                     SKIAMDVEAKHSQNGIAEWRYQDVPTKLWPIQPLRDFWGINRRTEAKLNKRGIFTIGD
                     LAKYPYKFLKKEFGILGVDMHLHANGIDQSKVREKHKISNPSICKSQILMRDYHFDEA
                     KVVMQELIEDVASRVRARKKVARTIHFAFGYSDEGGVHKQYTLKDPTNLEKDIYKVVM
                     HFADKLCNKQALYRTLSISLSQFINEDERQLSLFEDEYQRKRDECLAKTIDQLHLKYG
                     KGIVSKAVSFTEAGTKHGRLGLMAGHKM"
     gene            13698..13883
                     /locus_tag="P6L01_000752"
     CDS             13698..13883
                     /locus_tag="P6L01_000752"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDP3029837.1"
                     /translation="MPIVNVKLLEGRSDEQLKNLVSEVTDAVEKTTGANRQAIHVVIE
                     EMKPNHYGVAGVRKSDQ"
     gene            complement(13971..14954)
                     /locus_tag="P6L01_000753"
     CDS             complement(13971..14954)
                     /locus_tag="P6L01_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDP3029838.1"
                     /translation="MDKETNDNEYRRQSEHRTSAPKRKKKKKIRKLPIILLIVVILLI
                     ALVVYIVHSYNSGVEYAKKHAKDVKVHQFNGPVKNDGKISILVLGADKAQGGQSRTDS
                     IMVVQYDFINKKMKMMSVMRDIYADIPGYGKHKINSAYALGGPELLRKTLDKNLGINP
                     EYYAVVDFTGFEKMIDELMPEGVPINVEKDMSKNIGVSLKKGNHRLNGKELLGYARFR
                     HDPEGDFGRVRRQQQVMQTLKKEMVNFRTVVKLPKVAGILRGYVNTNIPDSGIFQTGL
                     SFGIRGEKDVKSLTVPIKNSYEDVNTNTDGSALQINKNTNKQAIKDFLDED"
     gene            15091..15600
                     /gene="msrA"
                     /locus_tag="P6L01_000754"
     CDS             15091..15600
                     /gene="msrA"
                     /locus_tag="P6L01_000754"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDP3029839.1"
                     /translation="MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYE
                     QVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDRGPQYQTAIFYT
                     NDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQK
                     IRQEYKNKQ"
     gene            complement(15803..18325)
                     /gene="mprF"
                     /locus_tag="P6L01_000755"
     CDS             complement(15803..18325)
                     /gene="mprF"
                     /locus_tag="P6L01_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDP3029840.1"
                     /translation="MNQEVKNKIFSILKITFATALFIFVAITLYRELSGINFKDTLVE
                     FSKINRMSLVLLFIGGGASLVILSMYDVILSRALKMDISLGKVLRVSYIINALNAIVG
                     FGGFIGAGVRAMVYKNYTHDKKKLVHFISLILISMLTGLSLLSLLIVFHVFDASLILD
                     KITWVRWVLYVVSFFLPLFIIYSMVRPPDKNNRFVGLYCTLVSCVEWLAAAVVLYFCG
                     VIVDAHVSFMSFIAIFIIAALSGLVSFIPGGFGAFDLVVLLGFKTLGVPEEKVLLMLL
                     LYRFAYYFVPVIIALILSSFEFGTSAKKYIEGSKYFIPAKDVTSFLMSYQKDIIAKIP
                     SLSLAILVFFTSMIFFVNNLTIVYDALYDGNHLTYYILLAIHTSACLLLLLNVVGIYK
                     QSRRAIIFAMISILLITVATFFTYASYILITWLAIIFVLLIVAFRRARRLKRPVRMRN
                     IVAMLLFSLFILYVNHIFIAGTLYALDIYTIEMHTSVLRYYFWLTILIIAIIIGMIAW
                     LFDYQFSKVRISSKIEDCEEIINQYGGNYLSHLIYSGDKQFFTNENKTAFLMYRYKAS
                     SLVVLGDPLGDENAFDELLEAFYNYAEYLGYDVIFYQVTDQHMPLYHNFGNQFFKLGE
                     EAIIDLTQFSTSGKKRRGFRATLNKFDELNISFEIIEPPFSTEFINELQHVSDLWLDN
                     RQEMHFSVGQFNEEYLSKAPIGVMRNEENEVIAFCSLMPTYFNDAISVDLIRWLPELD
                     LPLMDGLYLHMLLWSKEQGYTKFNMGMATLSNVGQLHYSYLRERLAGRVFEHFNGLYR
                     FQGLRRYKSKYNPNWEPRFLVYRKDNSLWESLSKVMRVIRHK"
     gene            complement(18806..20014)
                     /locus_tag="P6L01_000756"
     CDS             complement(18806..20014)
                     /locus_tag="P6L01_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDP3029841.1"
                     /translation="MNENEKNIRKNFLKFTESRYMKFVGGNDLVFSLIALVLLGIVIF
                     IFEKVSYVFDPFIIVFKTIAAPIIVSLILFYLFNPIVNMMERYRIPRVAGISIIYLAV
                     VGVITLIVNLLIPIIGSQVDSLVKNSPQYLEKLINSIDKIANNTFFSSYYSQINDWLN
                     SLPKKIPSMLSEFTDGFGSKIATFAETIANIGVVIVTTPFVLFFMLKDGHHFKEFSTN
                     IMPPKFRKDFHDLLEKMSVQVGSYIQGQIIVSFCIGILLFIGYSVIGLKYSLVLASIA
                     AVTSVVPYLGPTIAISPAIVIAAITSPWMLLKLAVVWTLVQFVEGHFISPNIMGKTLK
                     IHPLTIIFILLCAGKLLGIVGVILGIPGYAILKVLVTHLFQLFKRRYNRFYGNDVGEY
                     DIKESNKIVE"
     gene            complement(20007..20174)
                     /locus_tag="P6L01_000757"
     CDS             complement(20007..20174)
                     /locus_tag="P6L01_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029842.1"
                     /translation="MKKGLINPKLSVLHGRFVTVKGICMVSLMLVILHKWTVFIQHLM
                     NILSGGESIIE"
     gene            complement(20149..21000)
                     /locus_tag="P6L01_000758"
     CDS             complement(20149..21000)
                     /locus_tag="P6L01_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDP3029843.1"
                     /translation="MGEYIVTKTLNNNVVVCTNNDQEVILIGKGIGFNKKEGMALNDQ
                     TITIEKIYKLESEQQKAHYKSLVEIADDNVLQVIIDSLNFISNTAMNVDSKQLVVSLT
                     DHIIFAYKRLKQNQVISNPFVMETMQLYSDAYHIAKQVIDQLNAALDVHFPEDEIGFI
                     ALHIASNTEDLSMHEMTLINNVIKKGIDIIESDLVTTVDKESLQYQRFIRHVQFLIRR
                     LRRKEYIHAQDDFVSMIKNHYPICYNTAYKILTMIQKQFDVNISESEIIYLTLHIHHF
                     EERINQS"
BASE COUNT         6924 a         3808 c         2963 g         7486 t
ORIGIN      
        1 gctatatatt ttatgttttg cataatcttt taatcataaa actaaatcaa taaaacatca
       61 ttataatgaa aaacattaga ttgacaaata catactagtt tttcttcgtg tgaaataatt
      121 aaaatttcgc taaaaaaagc ataaaaaaga gcctatagta tcaataacta taagccctaa
      181 catcatttac atctatttct ttaatttttt acgtaattta tccaatgttg tgaacaacca
      241 atatttcact ttatgaattg gtttatagaa atcccctatt aattcttcga tttgtacatt
      301 aaatccacgt ttaaagcggt atacgccata atcttcactg ttttccgtaa aatcacctga
      361 taaaccatag aaattataac gatcatagcc attatcgaag caatagttaa tcataaacca
      421 atgcatcatg tatggtccca taaattgatt atatttttct gatgaaccac cagagaaata
      481 attcacttca tatgcatttg caaaataaac gccagaagca aggtttagaa ttgggccgtc
      541 cgttttgctc aattcacttg cattcaataa ttcatgctga tcatgatcaa tttgcttatc
      601 taattctgca attttcttca tttgtttatc tgatttgttt tctttcgcca tcatttgatc
      661 acgacgattt tctttgtcat tcaattcctg ttgcaacttt aacacatatt catcaaggtc
      721 aatatatgct aatggtacta atactttatc tccatatgtg tcaataaagt tataaaaata
      781 atcatctgtt tttgacacaa atccagcacg ctcttcagtt tcacgatata aatctaagaa
      841 aagattgaac tcatcacgtt caaggaatct gactttaaca ccatagttta tcgctttatt
      901 aatattacgt ttacgttgac tatcaaatgt ctttttcaat gtttcgggtg ttttaccttc
      961 aaggtttaat acgcccatcc atcgtacttg gctcgatgta tcatactcag ttgtaaagcc
     1021 atgatgctcg taaccatgtg atttaaacaa gtttactaag gcatcatttt tctcgcgacc
     1081 ttcaaatggc acgatatctt tatcatatag atgatataac caatacggat ctaatttaac
     1141 atataaacat tgatgttgct gtaaatattt atctaactct tttaaataat aatcaactaa
     1201 tcctaaatct gaaaaatcca ttactggacc acgattcgaa tagtaaacat aacttcccat
     1261 agtaggaatt ttagagaaaa ggcttgctgc aattactttg ttattgtcgt ctttaatacc
     1321 taataaaact acttcaaagc catcattctc acgggtaact atattttctt ttacttggaa
     1381 ataatgactt tccaatgatg gattttgtac aaagttgtca aattcggtaa cagttaactc
     1441 tgtaaatttc atgttttgat aattcccttc ctaaaaaatt ctgtctttaa cttttttaag
     1501 tgcggtatat gctgcgtaaa caggtttatt aattggttta ataaagtcac caacatattc
     1561 aataatttca gcattgtaac cttttttgaa tttaactaca ccagcatctt cagcatcttc
     1621 tgtaaattta ccactaacac catagaaatt ataacggtca atgccatgat ttaatgcata
     1681 attaatcatt tcccattgca ctgcataact tccggcaaaa tgacggaatg catttgatgt
     1741 accaccagca taataaacaa cttcaaatgg gttgataaag aagaaaccag cagagatagg
     1801 taattcatta ccatgttctt cttgtagacg tttaccttct tcaatctttt gctcattagc
     1861 atcaagttgt tgttgtaagt tatctcgctt gttgtgtgct tttttatttt caggacgttt
     1921 ttcaatatcc tttaacgctt tatttaaatc tttatttaaa atatcacgct cttcgtttag
     1981 ttctttaata tattcatcaa agttgatata cgctaaaggt actaacacac ggtctttgta
     2041 atattttaag cgattgtagt aaaatttgtc atcacgatca gcaaaagctt ttgattctga
     2101 cgtatcttcc ataaatgatc taaaaattgg cagttcttct tcagataaat atcttacttt
     2161 aacaccattc tttttaactt ttttcgtgtt tctttttcta agtccatcca tatttttaat
     2221 gatgtcatct gctgttttat cttttaaatc taacactgag tgataacgaa tttgtagcac
     2281 aggatcaaat cctttatgga atccagtatg ttcaaatcct aagttactca ttttatcaaa
     2341 gaaccaatca ttaccagcat tacctgtaat ctcgccatca tgattcaagt attgatatgg
     2401 taaatatgga tcgatatgta ggtatagaca acgatgtttt ttaacatatt ttgataattc
     2461 attaaagaaa aagtgtacga gttcttgatt ttcataatcg atcactggac cgcgatttga
     2521 ataaaaatac ttgaacactt tcataacagg tacagcagta agtaagcaag ctgcaatgac
     2581 ctcgttatta ttgtttttta ttcccactaa atgtgtttca taaccttcag caagctttaa
     2641 ctcatagtgg ccaacagttt gcgtgaaatg actgtatggc atgctatctg taaaggcacc
     2701 aaactcttta gctgttaaat ttgtaaactt cattatcatt actcctattt gtctctcgtt
     2761 aattaatttc atttccgtat ttgcagtttt tctatttccc ctctgcaaat gtcaaaaata
     2821 ataaatctaa tctaaataag tatacaatag ttaatgttaa aactaaaaca taaacgcttt
     2881 aattgcgtat acttttatag taatatttag atttttgcat acaatttcaa aaaaagtaat
     2941 atgaacgttt gggtttgctc atattacttt tgtatcattt ctattcaatt ttataattca
     3001 ccgtttttca ctttttcaaa cagtattcgc ctaatttttt taaatcaagt aaacttaatt
     3061 attcaatgtt tgttggatag attgtaaata tctaatgatt tcctcacgcg tgttagattt
     3121 aaatcgctta acgatttcgc taccaatgac gatgccatct gcaacctctt ttatatctgc
     3181 aacatgttgt ggtgttctta taccaaatcc tgcgacaact ggcacattgg ctatcgcttt
     3241 aattgactca atttttcgtt ttaattctgg atgaaacgca ccgttttgtc ctgttgtcgc
     3301 attcatcgtc acagtataaa taaagccttc cgcatgagat acgatatctt ttatacgttt
     3361 gtcatcagta gtcatcgcaa ctaacgatat gattttgacg ccatagtgac taaattgttg
     3421 ttttaaacgc tgcgataatt catatggtaa atcaggaata attaagccgt agacaccagt
     3481 atctcgacat ttttcaaaaa acgcttgttc tccataatga caaataatat tataatacgt
     3541 cattaataca tagttacact taatttgatc accatgtttt tctaattgat tgaaaatata
     3601 atctatcgtg atgccttgtt taatcgcttg ttgacctgct tccatgataa ctggaccatc
     3661 agcaaccgga tcagagaaag gtacaccaat ttcaattata tctgcaccat tttcactcaa
     3721 caatgttgca ttttcaatca aatctttatt gcccataata taaggtataa ataatttagt
     3781 catttgcaag acctcgctct gccatatatt gtctaattgt ttccatatct ttatcgccac
     3841 gtccagaaat agttactaca ataatatctt ctttcgacat cgtaggcgct agtctttcaa
     3901 cataactcag tgcatgtgca ctttcaattg caggtataat accttcatgt tttgtaaagt
     3961 tgattaaagc attcattgct tgtgtatcac ttgcattttc aaaagttact ctaccaatgt
     4021 cgtggtaata agaatgttca ggcccaatac caggataatc aagtcctgct gaaatagaat
     4081 gtgctagttg cacttgccca ccttcatctt gaattaaata cattttagta ccatgtaata
     4141 cgccaggtga tcctttgcca attgcaagtg catgtttatc agtatcctcg ccttgacctg
     4201 cggcttcaac accgtataat gcaacatcat ctttaataaa tggataaaat gtaccgattg
     4261 catttgagcc accaccgata catgctacaa ttgcatccgg aagtcgacct tctttcttca
     4321 atatctgtga ttttatttct ttaccaatga cactctgaaa atctctaaca atcgttggga
     4381 atgggtctgg acctaatgca gaacctaata aataatgtgt atcatctaca tgacttaccc
     4441 aatattgcaa tgctttatta actgcatccg ataaagtccc ttgaccttct tcaactgcca
     4501 caacctttgc accaagtaat tccattctaa atacattaag ttgttgtctt ttaatatctt
     4561 cacttcccat aaagacaaca agttccatat caaataatgc agcaaccgta gcactagcta
     4621 caccatgttg acccgcacca gtttcagcaa caagcttctt cttgcccatt cttttagcaa
     4681 gcaacgcttg acctaacgca ttattaattt tatgggcgcc tgtatgattt agatcctctc
     4741 gtttcaaata tattttagcg ccacctaggc tttcagtata tgatgcagca tatgtaagtg
     4801 gtgtcgcgcg tcctacatac tctgataaat agtattccag ttctctttga aactctgggt
     4861 ctgcttttgc ctctttataa gcttttttca actcaataat tgctggcatt aatgtttctg
     4921 gaacatattg ccctccatat tcaccaaaga aacctaattc atctgcttct gtttgtattt
     4981 gtttattcat tgtctctatc tcctttcaca atatttacaa ttgctgtcat tttttctata
     5041 tcttttcgcc catttacttc tatacctgat gcaagatcat aaccttgatg tgataattta
     5101 agttgattaa ctgtttgaat attttcagag ttaatgcctc ctgctatcaa ataaggtatg
     5161 tcttttatgt gcttcaaaat agtccagtca tatgtttgac cggtaccacc atacgacact
     5221 gagggtgtgt cgataataaa taaatctacg aaccctttat atttatttat gttttggatt
     5281 atgttttcat ctgcagctaa agctttagtg attttaatgc ttgaatattt ctttttaatt
     5341 tcctgtataa aatcaataga ttctgtgccg tgtaactgta ttgtgttaat tgacgtattg
     5401 cttaatacgt gttcaattgt tgttaaatca ggatttacca tgacacatac tttatcgata
     5461 tgatttggaa cagcagacgc taactttttt atttgggtaa ttgtttgatg ccttttactt
     5521 ttttcataat ggatgaaacc tatcgcatca ataggtaatt gactggccgc tgtaacatcc
     5581 tttattgatg taaagccaca aaatttcaat ttcatcatga cttcaccttt tgcattttca
     5641 gttgtggtaa aaattcagat agattgtcac aacgcataag cgcctcacct attagtaagc
     5701 catcgatacc actatgcaag atttttctta catcagatgc atcgtgaata ccactttcag
     5761 aaatataatg atgattgggt tttttatttt ctaaaatagt atttgtatgt tccacatttg
     5821 tgacaaatcg ttttaagtcc ctgttattta caccaatcaa tttagcatta accttatagg
     5881 cacgttctaa ttcatggcga tcatgtactt caattaacac ttctagattt tgcgatatag
     5941 cgtagttata taaatctttc aattgtttat ccgataagat gttaacgatc aataaaatca
     6001 tagatgcacc agcttgttta gcaacatcaa tttgaagcgg gtctataata aagtctttgc
     6061 ataatacggg taatgttgtt tttgttgtca atgcttgtaa tctttcaaaa ctaccaccaa
     6121 agtacttttc atcagttaaa atggacacgg catttgcacc gtacttttca taatctgaga
     6181 tttgttgcga taaatctcgt tcaggtaagt cattaactgt aggactcttc gatttaattt
     6241 ctgcaataat tgctagcttt ggttctttct caattgcgtt tataaaagat tttttattct
     6301 gaatcttcac acttttcaag gtattaagtt tgtcttgata atagccattt tgtaaaagtg
     6361 actgtttata tttaacaatt tctgctaaaa tcgtcatatt attcacctct catttgatgg
     6421 tattttttca atgcttcacc attatcaatc aatgtagttg caagttctat gccttctgcg
     6481 atggtatcca ctttctctgc aacataaagg cttaaacccg catttagtaa gacaacatca
     6541 cgtcgacttg actgatcttt accattcaag atattaaggg agattgctaa attttcttca
     6601 ggtgaaccgc ctttaaaatc actattcggc gcatgtttca aaccataatc agtcgcattt
     6661 aatgtgtaat ttttgatttc tccatcttca gtcaattcat atatcaaatt atcaccagat
     6721 agtgttgctt catccatacc atttgcacca tgtaaaacga ttgcacgttt tctacctaaa
     6781 tctttaatcg ttttagcaac taactttaac tttgtaggat caaagacgcc taccatttga
     6841 tacgttaagt gatatggatt aattaatgga cccacaaggt ttaatattgt aggctttcca
     6901 atcatttttc taactggttg catatacttc atgattggat atgattcagt tgcaccaatg
     6961 aatacaaggt ctttttcatt taattggtta ggtgtatcat caacagttgt tgtttgtatg
     7021 ttcatttgat ttaacaaatc cgtactacct gaatttgagg taatactttt attaccatgt
     7081 tttataactt ttacgccagc acttgctaca acaaaagcaa cagtcgttga aatgttgaaa
     7141 ctatttgact tgtcaccacc tgtgccgcac acacacatag ccccttcata acatggttga
     7201 tgtggataca ttgtattaat taagctacgt acaatatatg ttaattcttg ttgttggatt
     7261 tctcgctccg aataggaact aagtaattca tatttaatat cagttccaat actaggagaa
     7321 ataagtatat cgattagctc tttaatgtcg ctttcaagta atatagtttc agtttttatt
     7381 cttgttagta atgtcatggt tttcatcctt ccttcactag attaatgaaa tttgtaatta
     7441 ttttgacacc atagtctgta gcaaatgatt caggatggta ctgaataccg taatgcggtc
     7501 tttctttatg ctcaaatgac tgtatacaat cttcggtacg tccagtaatt ttcaattctt
     7561 ctggaaaatt gtcagggtta cttattaatg aatgatatct cataattgaa aactgttctg
     7621 gtatatcttg atataacaga tgttgatgat gcgatataac ctttagtgta tcaacttttc
     7681 cgtgcataac cttgtcgcct ttaatgactt ctccaccgta ataacaagtc agtgcctgag
     7741 cacctaggca aatacctaaa atgggtttgt gttgataggt tgatatgatt ttcattaact
     7801 gttgatcgtc taatggatgc cctggaccag gagatataat aacagcgtcc accgattgat
     7861 tcagcacatt atcatcatca ggatattgaa caatgacgtc agtatgttga gcaacaatat
     7921 ccactaggtt atatgtaaag gaatcataat tatctacaac taagatcatg gcctcacctc
     7981 caataagctt ttagctttca atttcgtttc attcagttct ttttcaggaa tagaatcata
     8041 tacaacgcca caaccagctt ctacgttgat atactgctca tctatcatca tcgttcgaat
     8101 tgctaaagca aaatctaagt tatgattaca atttatgtat ccaacaccac cactataaac
     8161 gcctcgttta tgtggatatt gttcatatat tctttcaatt gcacgtaatt ttggtgcacc
     8221 tgaaacggta cctgttggta ataaattcgc aataactgtc attggtgata aattttgatt
     8281 tattttacct gtgatttcac ttacgatatg cataacatgt tcatattttt caataaccat
     8341 taatttagta atttttgagg taccgatttt acttactcta tgaatatcat tacgtcctaa
     8401 atcaactagc atacgatgtt cgctgcattc ttttggatca ttaagtagtt gtttcatatt
     8461 ctcattatct atttgtgtcg tctcaccacg ttgaatcgta cctgcaatag gattagttgt
     8521 tacaatttga tctttgacac ttacgaaact ttcgggagaa ctaccgacaa tatatggttg
     8581 atcgatatta agataataca tatatggact tgggttttgt cgttttaaat tttgatacag
     8641 ttgaaacgaa agttgattta aatgctgact agcatgatgc gcatatttgt aaattcttga
     8701 tggcacaact tggaacatat ccccttctgt tattttctct ttgaaatact gaatcatttc
     8761 gataaatctt tcttcagaaa tatttgattg aatttcttta gttttaaaat caaaatcttg
     8821 tgtaggcatg aatggttgga ttttagttaa gtcttcgata gacttattaa ctcgattctc
     8881 aagatctgat tttgttgaat ttgaaaattg attcgtcgcg atgatatata actcatcttt
     8941 gtaatggtca aatacataaa cttgttcaac catatataac cttacatcgt gctgcttgtg
     9001 atcttctaat tgtattgatt gcaatttagg aaattcatgt cttactaaat caaagctaca
     9061 cgtcccaaca tatccagata taaacggtaa tgacttaagt tgctcatctt gaatattatg
     9121 gtagtcttca tttattttag tcgttaaata atggtacggt ctttcaatga tttgatacga
     9181 ttcttttaaa gtgcttactg ataatacatc attatctaaa gttaaagtgc cataaatatc
     9241 aaaaataaca actgaatagc gacctttagt ttgttgttga tttgtacttt ccaaaatgat
     9301 cttcttggaa tgaagttgtg ctaaaacttc gggcgttaca tttgctttta tttttttata
     9361 aaatatatcc attctatacg ctcctttcaa aaaagcgcca gcatccttat caatataaag
     9421 gacgctgacg cgtggtacca cctaaattaa ccgaatattc aaaatattca tagttcactc
     9481 tttactatat ataatttttc tgttgcagac aaaacccaat ttcattaatc tcggtgttag
     9541 ttcacatcat ccactaactt tctgttgcta cctttaatta actactacat tttgtctaaa
     9601 taaaaagaca ccacataacg catatacctg taaggacgcg ttaccgtggt gccaccttag
     9661 ttaacataat tcatgttcac tttcaaaact cattcaattg ttttccaaag tccaattcac
     9721 aagatatggc gtgctaattc ccatcaccca ttagctttct atttgctacg tttcatatct
     9781 gctactattt ctttagaaaa ttctaattat ttttatagtt cattatatta cataggtcgt
     9841 tcacatttgt caacactaat tgtttactca attggtgata agcaatatgc atatagtaat
     9901 ttctctgtcg ctttaataga atcaatatgt gttcgttcca ttgcatgaga tgattcaatg
     9961 ccagcgccaa ataaaccatg tctgatatcc gcaccagcat gtaaagctgc tgaagcatct
    10021 gaaccataat atggatatat gtctacttta tatggaatgt tatttatttt gcaaagatta
    10081 actaggtgcg atttcaattg cttatgatat ggacctgaag catctttggc acaaatagaa
    10141 actgtatatt catccgatgc ttgaccgtct cccaacgcgc ccatatctaa tgcaatatat
    10201 tctttgattt tcgaatcaat tgatgcattt gcaccgtaac ctatttcttc gttattagaa
    10261 atataaaatt gcgttgtatg tggtaatatt atttgctctt cttttaattt ctttagtaat
    10321 tgtagtatca tcgctacgct agctttatca tctaaatgac gagatttaat aaaacctgat
    10381 gacgtgataa ctgtacgtgg atcaaagcta acaaaatcac ctactgaaat acctaaactc
    10441 tttgtatctt cttctgatgt agttacttca tcaattctta tttccatatg cttttgatct
    10501 ctaggtattt catgattatt tctatataca tgaacacttg tttcatgcag acaaattgtt
    10561 cctgtatata tttgaccagc atcagtttta atttggcaat attcaccctc aatcgcgtta
    10621 tacgtgaatc ctccaattaa ttctattgct aagcgaccat cttctttaat ttctttaacc
    10681 attgcaccta aagtatcaac atgagcagta atacagcgtt gtacttcatc atttttgcct
    10741 ggcactgtta ttaataacgc acctttattt gttagtgttg tttgataacc caagtctttt
    10801 gcatattttt cgacaaattg aattgcttct tctgtgtctc ctgaaggact attaatacct
    10861 gttaatgttt taatcatttc taatattggt tccattgatt tacccctccg ttgccattgt
    10921 gttactatac tctttattgt agatgattta tctccgttta tctattaatg ctattatcga
    10981 tttttattta ctattgcact gtgtcaacag ctcatttaca atgttattat tctgaaaatt
    11041 tcgaaataag gtgatttata tgacaacagt tttatttgtt ggacttggat taattggtgg
    11101 aagtcttgct agcaatataa aataccataa ccctaatact aatattattg catacgatgc
    11161 agatacttct cagttagata aagctaaatc aatcggcatt attaatgaaa aatgtttaaa
    11221 ttatagtgaa gctattaaaa aagccgatgt aattatttat gcaacacctg ttgctatcac
    11281 aaataaatat cttagcgagc ttatagatat gccaactaaa cctggtgtta ttgtttctga
    11341 tactggtagt actaaagcaa tgatacagca acacgaatcc aatttattaa agcataatat
    11401 tcatttagtc agtggtcatc caatggctgg tagtcataaa tctggtgtac taaatgctaa
    11461 aaagcactta tttgaaaacg cttattatat tttagtctat aatgagccaa gaaatgagca
    11521 agcagcaaac acgttaaaag aactgttatc acctactctt gctaaattta ttgtaactac
    11581 tgctgaagaa cacgactacg taacaagcgt cgtaagtcat ttacctcata tcgttgcatc
    11641 tagtttagtt catgttagtc aaaagaacgg tcaagaacat catttagtta ataaacttgc
    11701 agctggtggt tttcgtgata tcactcgtat agctagtagt aatgcacaaa tgtggaaaga
    11761 tatcaccttg agtaataaaa cgtatatttt agaaatgatt cgacagctaa aaagtcagtt
    11821 tcaagattta gaaagactaa ttgaaagcaa tgattctgaa aaattgttat cattttttgc
    11881 ccaagctaaa tcgtatcgcg acgcactacc cgctaaacaa ctaggtggac taaatactgc
    11941 gtatgatcta tatgtagata ttccggatga atcaggtatg ataagtaaag tgacttatat
    12001 catgagttta cataacatat ctataagcaa cttaagaatc ttagaagtac gcgaagatat
    12061 atacggtgct ttaaaaatta gtttcaaaaa tcctactgac cgagaacgcg gtatgcaagc
    12121 attgagtgat tttgattgtt atatccaata attattaagt ctcttttgat aaattcccat
    12181 gtattcttag atattattgt gaaatctatt gtgatatatg aaatatttat ctttagagac
    12241 ttattttata cgaatcacag ttatatattc cttaaaccgt atagtcatta cattttatgt
    12301 ccagccatta aacctaatct accatgtttt gtacctgctt ctgtaaacga tactgctttg
    12361 gacacaatac ctttgccgta tttcaaatgt aattggtcta tcgttttagc taggcattcg
    12421 tcacgtttgc gttggtattc atcttcaaac agacttaact gtcgctcatc ctcattaata
    12481 aattgactca aagatatact tagcgtacga tatagtgctt gtttattaca taatttatct
    12541 gcaaaatgca ttactacttt ataaatatct ttttctaagt ttgttggatc tttcaaagta
    12601 tattgcttat gtacaccgcc ttcatcacta tagccaaagg caaaatgtat cgttcttgcc
    12661 actttttttc ttgctcgaac tctgctagca acatcttcaa ttaactcttg cattactact
    12721 tttgcttcat caaaatgata atctctcatt aatatttgac ttttgcatat cgatggattg
    12781 ctgatcttgt gcttttcacg tactttactc tgatctatcc cattcgcatg tagatgcata
    12841 tcaacaccta aaataccgaa ctcttttttt aaaaatttat atggatattt cgctaaatct
    12901 cctatagtaa aaattcctct tttattcaat ttggcttctg ttcgacgatt aataccccaa
    12961 aaatctcgca agggctgaat tggccataat ttcgttggta catcttgata tcgccattca
    13021 gctataccat tttgactatg cttcgcttca acatccatag caattttact taataacata
    13081 ttagaaccaa tgcccacagt acaataaatg cctgtttctt cataaatttc acgtttaagt
    13141 ctttcgcaaa atgcatgtac tgtagaacta aatctatgat agctatcagt aacatccata
    13201 aaaaattcgt caatactata ttggtgtaaa tcttcaggtg gaatataacg caatgcaatc
    13261 ttagaaatag caactgaaac attaagatat ttacgcatac ttggattgat aatgtaaata
    13321 tcatttctat gtggtatttc aaacaatcgc gaccctgtct tgatgcctaa ttcttttaat
    13381 ttaggtgtcg cagccaatat tacagaaccc tgacgcttag tatctgcaac aacagctaac
    13441 tttgtttcta atggatctag ccccttttca atacaagaaa cactcgcaaa aaaacttttt
    13501 tggtcaatac atagaacatc cctatcttct aataaatgat aattatacac tgttattcct
    13561 cccttacttg aaccttatta tatacgaaca cgtgttctat ttcaactatt ttagaacaaa
    13621 tgttctgtta ttttgcaaat gaatttttaa gtattacatg atatactcaa attaataatg
    13681 attaggaggt ttggatgatg ccaatcgtca atgtaaaatt attagaaggt cgttcggatg
    13741 aacaattaaa aaatttagtt agcgaagtaa ctgacgccgt agaaaaaaca acgggggcaa
    13801 atagacaagc aattcacgtt gttatagaag aaatgaaacc aaaccattat ggtgtggctg
    13861 gcgtaagaaa gtcagatcaa taattcttca taagatgcat gccaattaat tctttgaaaa
    13921 cgaacaaggc gacttctatc tgagtatgat agaaatcgcc ttgtttattt ttaatcttca
    13981 tctaaaaagt ctttaatagc ttgtttattt gtgtttttat taatctgtaa tgcactacca
    14041 tcagtatttg tattgacatc ttcgtatgag ttcttgattg gcacagtcaa tgacttaaca
    14101 tctttttcac ctcggatgcc aaaactcaaa cctgtttgga aaatccctga atcaggaatg
    14161 tttgtattca catagcctct taaaatacct gcaacttttg gtaatttaac aactgttcta
    14221 aaattaacca tttctttttt caatgtttgc atcacttgtt gctgacgtcg cacgcgtccg
    14281 aagtcacctt cagggtcgtg acggaatctt gcataaccaa gtaattcttt accattcaac
    14341 ctatggttac cctttttcaa agatacacca atatttttcg acatatcctt ttcgacatta
    14401 attggtacac cttctggcat taattcatca atcattttct caaatccagt aaaatcaact
    14461 actgcataat attcaggatt aattcctaaa tttttatcaa gtgtttttct aagtagctct
    14521 ggaccaccta aagcgtatgc tgaattaatt ttgtgttttc catatcctgg aatatctgca
    14581 taaatatcac gcatgacaga catcattttc atttttttat tgataaagtc atattgaaca
    14641 accatgatag aatctgttct tgattgtcca ccttgtgctt tatctgcacc gagtacaaga
    14701 atagaaattt taccatcatt ttttactggt ccattaaatt gatgtacttt aacatctttc
    14761 gcatgtttct tggcatattc tacaccgcta ttgtaactat gtacaatata tacaactaat
    14821 gcgataagta aaattacaac aatcagaaga atgataggta atttcctaat tttcttcttc
    14881 ttttttcgct taggcgccga agtgcgatgt tcactttgac gtctatattc gttgtcatta
    14941 gtttctttat ccatatttac ctaccttata tcttcaaaaa tagatttttg tgtactataa
    15001 taatcacata ttaacgttat ataaaaacaa ttcaaaaagc aagtgatttc ttaataaatc
    15061 gtactaaggt ctaatgaaag gatgtcaaaa atgaatatta atacagctta ttttgccgga
    15121 ggttgctttt ggtgtatgac gaaaccattt gacacctttg acggcataga aaaagtaact
    15181 tctggatata tgggcggaca tattgaaaat cctacttacg aacaagtaaa atcaggtacg
    15241 agtggtcatt tagaaactgt tgaaattcaa tatgatgttg cattattctc atacaataag
    15301 ttattagaaa tatttttctc agtcattgac ccattagata caggtggtca atatcaagac
    15361 cgtggtcctc aatatcaaac agctattttc tacactaatg atcatcaaaa agaactcgct
    15421 gagacatata tcgagcagct taaaaatacg attaatgctg ataaggcaat tgcaacaaaa
    15481 atattaccag cgtcacaatt ttacaaagcc gaagactatc accaagattt ttataagaaa
    15541 aatccagagc gctatgcaga agaacaaaaa atacgccaag aatacaaaaa taagcaataa
    15601 catttatact tgatgaaact gattgggctg ataataaaag ttaggcgact taacttaagc
    15661 tcatttcaat attaaaatac ggtacagata ggaaacttaa atattcttat ctgtaccgtt
    15721 ttttataaat cattctttat agaaaccaaa aaatccaagc gcttcaggca taactgtata
    15781 cctcttagca cttggatttt aattatttgt gacgtattac acgcattact ttagaaagtg
    15841 attcccaaag cgaattatct ttacgataaa ctaaaaagcg tggttcccaa ttcggattat
    15901 atttagattt ataacgacgt aatccttgga aacgatatag accgttgaaa tgttcaaaga
    15961 cacggcctgc aagtcgttct cttaaatatg aataatgcaa ttgaccaacg ttcgataacg
    16021 ttgccatacc catattaaat tttgtataac cttgttcttt actccaaagt aacatgtgca
    16081 agtataatcc atccattaat ggtaaatcta actctggcaa ccatctaatt aaatcgactg
    16141 aaatggcatc attaaagtat gttggcatta aactacagaa tgcaattact tcattttctt
    16201 catttcgcat tacaccaatt ggcgctttag ataagtattc ttcattaaat tgacccacag
    16261 agaaatgcat ttcctgacga ttatctagcc ataaatcact tacatgttga agctcattta
    16321 taaattcagt tgaaaacggt ggttcaataa tttcgaatga aatattaagt tcatcgaatt
    16381 tgtttaaagt cgctctaaat ccacggcgtt ttttacctga agttgaaaat tgcgttaaat
    16441 caataattgc ttcttcacct aatttgaaaa attggttacc gaaattatga tataaaggca
    16501 tgtgttgatc tgtaacttga tagaatataa catcatagcc taaatactca gcgtaattat
    16561 agaatgcttc taacaattca tcaaaggcat tttcatcacc taacggatct ccaagaacca
    16621 ctaatgaact tgctttataa cgatacatta aaaatgctgt tttattttca ttagtgaaaa
    16681 actgcttgtc accactatat atcaagtgac tcaaataatt accgccgtac tgattaataa
    16741 tctcctcgca atcttcaatt ttagaagaaa tacgtacttt gctaaattga taatcaaaca
    16801 accatgcaat catacctatg atgatagcga tgattaaaat cgtaagccag aagtaatagc
    16861 gcaatacaga tgtatgcatt tcaatcgtat aaatatctaa tgcatataac gttccagcaa
    16921 taaatatatg gttaacatat aaaataaata aactgaataa aagcattgca actatatttc
    16981 tcattcttac tgggcgtttc aacctacgtg ctctacggaa agctacaata agcagaacaa
    17041 aaataatagc taaccatgtt attaaaatat atgaagcgta agtgaagaat gtcgccactg
    17101 tgattaataa aattgaaatc atagcaaaga taatggcacg tctactttgc ttataaatac
    17161 caactacatt cagtaaaagt aataaacaag cactagtatg aattgccaat agaatataat
    17221 acgttaagtg atttccatca tataaagcat cgtaaacaat cgttaagtta tttacaaaaa
    17281 agatcatact tgtaaagaat actaaaattg ctaatgataa tgatggaatt ttagcaataa
    17341 tatccttttg ataagacatt aaaaatgacg taacatcttt agcaggaata aagtatttag
    17401 atccctcaat gtacttctta gctgatgtac caaattcaaa tgatgataaa attaatgcaa
    17461 taattaccgg tacaaaatag tacgcaaaac gatatagaag tagcattaat aatacttttt
    17521 cctcagggac acctaaagtt ttaaatccta gtaatacaac taaatcgaaa gcgccgaaac
    17581 caccaggaat aaagctgact aaacctgata atgcagcaat gataaatatt gcaataaagg
    17641 acatgaatga tacatgagcg tcaacaatta caccacagaa atataataca actgcagctg
    17701 ctaaccattc aacacacgac actaaagtgc agtacaatcc tacaaaacga ttgtttttat
    17761 cgggtggtct aaccattgaa taaataatga ataatggtaa gaaaaatgac actacatata
    17821 atacccatct tacccatgta atcttatcta agattaaaga tgcatcgaaa acatggaata
    17881 caattagcaa tgataataag cttaaacctg tcaacattga aataagtatt aaggatataa
    17941 agtgaactaa tttcttttta tcatgcgtat agtttttata aaccattgct ctaacgcctg
    18001 caccaataaa gccaccgaaa cctacaatcg cattcaatgc attgatgata taacttactc
    18061 ttaaaacttt gcctaaggat atatccattt ttaaagctct agataaaatc acatcataca
    18121 ttgatagaat aacaagcgat gccccaccac caataaatag taacactaag gacatacggt
    18181 taatcttact aaattcaacc aacgtatctt taaagttaat accagataac tcccgataca
    18241 atgtgattgc tacaaaaata aataaagctg tagcaaacgt aatttttaag attgaaaata
    18301 ttttgttttt aacttcctga ttcatttttt cacatcaatt ctaattattt ctgttataaa
    18361 tcaaaatata tcatataaac cataaaaata gcatatcatt tacctttgat ttatataaca
    18421 cttttgtcat attgatatct gtattaagaa gtaaatagaa aatatttcta tctcaaacag
    18481 aacaatatgg ttatctatac tgattcatat ttaacataaa gaaaatgatg aatcaatact
    18541 tatgaaaaat attattaatt aacagccgat tatgagtgct tcttaatata ttaaaaatac
    18601 cacttatttt tattttcgac ataataatga agtactaatc aagtggcaaa tactcgtttc
    18661 attatgcatg tgctttataa acggctaaac tttcgtaaaa atttataagc taaacatgtg
    18721 ctgataatca taaatgtaac tgcccaacat tataagcacc ataaaaaata ccaacaaaca
    18781 tatagtcatt ggtatttttt attacttatt caactatttt attactttct ttaatatcat
    18841 attcacctac atcattacca tagaaacgat tgtatcgacg tttaaataat tggaataaat
    18901 gagtaactaa tacttttaaa atagcatatc ccggaatacc taaaataacg cctacaatac
    18961 caagcaattt gcctgcacac agtaaaatga aaatgattgt aagtggatga atcttaagtg
    19021 ttttacccat gatatttggt gaaatgaagt gcccttcaac aaattgtact aaagtccata
    19081 ctactgctaa ttttaagagc atccacggcg atgttatagc agctattaca atagctggag
    19141 aaatcgctat agtaggccct aaatatggta caacacttgt aactgccgca atactagcta
    19201 atactaagct atatttcaac ccgataaccg aataaccgat aaacaacagt ataccgatac
    19261 agaatgaaac gataatttgt ccttgaatgt atgaaccaac ttgaacactc attttttcaa
    19321 gtagatcatg aaaatctttt cggaatttcg gtggcataat attcgttgaa aattctttga
    19381 agtgatgtcc atctttaagc ataaagaata gtacaaatgg tgttgtgaca atcacaacgc
    19441 caatattagc aatcgtttct gcaaacgttg caattttaga cccaaagcca tctgtaaatt
    19501 cacttaacat agatggtatt ttcttaggta aagaatttaa ccaatcatta atttgactat
    19561 aatacgaaga gaaaaacgta ttatttgcta ttttatcaat agaattaatt aatttttcta
    19621 gatattgcgg tgaattttta actaaactat ctacttgcga accaataata ggtatcaata
    19681 aattaacaat taacgtaata acacctacta cagctagata aataatagaa atacctgcaa
    19741 ctcttggtat tctataacgt tccatcatat ttacgattgg gttaaataga tagaatagaa
    19801 ttaaagagac gatgataggt gctgctatcg tcttaaaaac gatgataaaa ggatcaaaaa
    19861 catatgatac tttttcgaaa ataaaaataa caatacccaa caatactagc gctattaatg
    19921 agaagactaa atcattccca ccaacaaact tcatataccg tgattcggta aattttaaaa
    19981 aattctttct tatattcttt tcattttcat tcaattatag attcaccacc acttaaaata
    20041 ttcataagat gttgtataaa aacagtccac ttatgtaaaa ttactaacat caatgatacc
    20101 atacaaatac cctttacagt aacgaaacgc ccgtgtaata cacttaattt aggattgatt
    20161 aatcctttct tcaaaatgat gaatgtgtaa tgttaaatat ataatttcag actcactgat
    20221 attaacatca aattgttttt gtatcatagt taaaatttta tatgctgtgt tatagcaaat
    20281 cggatagtga tttttaatca tagacacaaa atcatcttgt gcatgtatat attcttttct
    20341 tcttaatcgg cgaattaaaa attgtacgtg ccttataaaa cgttggtatt gtaatgattc
    20401 cttatcaaca gttgtcacaa ggtctgattc aatgatatct ataccttttt taataacatt
    20461 attgatcaag gtcatctcat gcatagataa atcttctgta ttagatgcaa tatgtaatgc
    20521 aataaatcct atctcatctt caggaaaatg tacatctaat gctgcattta actgatcaat
    20581 cacctgttta gcaatatgat atgcatcact atataactgc atagtttcca taacaaatgg
    20641 attgctaata acttgatttt gttttaagcg tttataagca aatataatat gatccgttaa
    20701 tgaaactaca agttgttttg aatcaacatt catcgcagta ttagaaataa aattcaacga
    20761 atcaataatt acttgtaata cattatcatc agcgatttca actaaacttt tataatgtgc
    20821 tttttgttgc tcactttcta atttataaat tttctctatt gtaatagttt ggtcgtttaa
    20881 cgccattccc tcttttttgt taaaaccaat acctttaccg attaaaataa cttcttgatc
    20941 attattagta catactacga cattgttgtt caatgtttta gtaacaatat attctcccat
    21001 tatcatcacc tatttttcta ttattaagat tatatatcgg aaatgtctaa cttgaaagta
    21061 taataattta aatacttgtg ttctattaca cgcctatcct atatgatata tcttaattta
    21121 atttcgatgt ctctcaaagt ggaataacta taataaaaaa atctgactcc catatttaca
    21181 a
//
LOCUS       DAOBBH010000028        20964 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.028, whole genome shotgun sequence.
ACCESSION   DAOBBH010000028 DAOBBH010000000
VERSION     DAOBBH010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20964
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.028"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            61..729
                     /gene="capA"
                     /locus_tag="P6L01_000759"
     CDS             61..729
                     /gene="capA"
                     /locus_tag="P6L01_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     protein CapA"
                     /protein_id="HDP3029844.1"
                     /translation="MESTLELTKIKEVLQKNLKILIILPLLFLIISAIVTFFVLSPKY
                     QANTQILVNQTKGDNPQFMAQEVQSNIQLVNTYKEIVKSPRILDEVSKDLNDKYSPSK
                     LSSMLTITNQENTQLINIQVKSGHKQDSEKIANSFAKVTSKQIPKIMSVDNVSILSKA
                     DGTAVKVAPKTVVNLIGAFFLGLVVALIYIFFKVIFDKRIKDEEDVEKELGLPVLGSI
                     QKFN"
     gene            745..1431
                     /gene="cap8B"
                     /locus_tag="P6L01_000760"
     CDS             745..1431
                     /gene="cap8B"
                     /locus_tag="P6L01_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8B"
                     /protein_id="HDP3029845.1"
                     /translation="MSKKENTTTTLFVYEKPKSTISEKFRGIRSNIMFSKANGEVKRL
                     LVTSEKPGAGKSTVVSNVAITYAQAGYKTLVIDGDMRKPTQNYIFNEQNNNGLSSLII
                     GRTTMSEAITSTEIENLDLLTAGPVPPNPSELIGSERFKELVDLFNKRYDIIIVDTPP
                     VNTVTDAQLYARAIKDSLLVIDSEKNDKNEVKKAKALMEKAGSNILGVILNKTKVDKS
                     SSYYHYYGDE"
     gene            1434..2198
                     /gene="cap8C"
                     /locus_tag="P6L01_000761"
     CDS             1434..2198
                     /gene="cap8C"
                     /locus_tag="P6L01_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8C"
                     /protein_id="HDP3029846.1"
                     /translation="MIDIHNHILPNIDDGPTNETEMMDLLKQATTQGVTEIIVTSHHL
                     HPRYTTPIEKVKSCLNHIESLEEVQALNLKFYYGQEIRITDQILNDIDRKVITGINDS
                     RYLLIEFPSNEVPHYTDQLFFELQSKGFVPIIAHPERNKAISQNLDILYDLINKGALS
                     QVTTASLAGISGKKIRKLAIQMIENNLTHFIGSDAHNTEIRPFLMKDLFNDKKLRDYY
                     EDMNGFISNAKLVVDDKKIPKRMPQQDYKQKRWFGL"
     gene            2218..4041
                     /gene="cap8D"
                     /locus_tag="P6L01_000762"
     CDS             2218..4041
                     /gene="cap8D"
                     /locus_tag="P6L01_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8D"
                     /protein_id="HDP3029847.1"
                     /translation="MAHLSVKLRLLILALIDSLIVTFSVFVSYYILEPYFKTYSVKLL
                     ILAAISLFISHHISAFIFNMYHRAWEYASVSELILIVKAVTTSIVITMVVVTIVTGNR
                     PFFRLYLITWMMHLILIGGSRLFWRIYRKYLGGKSFNKKPTLVVGAGQAGSMLIRQML
                     KSDEMKLEPVLAVDDDEHKRNITITEGVKVQGKIADIPELVRKYKIKKIIIAIPTIGQ
                     ERLKEINNICHMDGVELLKMPNIEDVMSGELEVNQLKKVEVEDLLGRDPVELDMDMIS
                     NELTNKTILVTGAGGSIGSEICRQVCNFYPERIILLGHGENSIYLINRELRNRFGKNV
                     DIVPIIADVQNRARMFEIMETYKPYAVYHAAAHKHVPLMEDNPEEAVHNNILGTKNTA
                     EAAKNAEVKKFVMISTDKAVNPPNVMGASKRIAEMIIQSLNDETHRTNFVAVRFGNVL
                     GSRGSVIPLFKSQIEEGGPVTVTHPEMTRYFMTIPEASRLVLQAGALAEGGEVFVLDM
                     GEPVKIVDLARNLIKLSGKKEDDIRITYTGIRPGEKMFEELMNKDEVHPEQVFEKIYR
                     GKVQHMKCNEVEAIIQDIVNDFSKEKIINYANGKKGDNYVR"
     gene            4031..5059
                     /gene="cap8E"
                     /locus_tag="P6L01_000763"
     CDS             4031..5059
                     /gene="cap8E"
                     /locus_tag="P6L01_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8E"
                     /protein_id="HDP3029848.1"
                     /translation="MFDDKILLITGGTGSFGNAVMKRFLDSNIKEIRIFSRDEKKQDD
                     IRKKYNNSKLKFYIGDVRDSQSVETAMRDVDYVFHAAALKQVPSCEFFPVEAVKTNII
                     GTENVLQSAIHQNVKKVICLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLIC
                     GTRYGNVMASRGSVIPLFIDKIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAET
                     GDIMVQKAPSSTVGDLATALLELFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMG
                     DYFRVPADSRDLNYSNYVETGNEKITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNEL
                     NDYKASMR"
     gene            5072..6181
                     /gene="cap8F"
                     /locus_tag="P6L01_000764"
     CDS             5072..6181
                     /gene="cap8F"
                     /locus_tag="P6L01_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8F"
                     /protein_id="HDP3029849.1"
                     /translation="MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESAL
                     LKADFIVHLAGVNRPEHYKEFSLGNVSYLDHVLDILTRNTKKPAILLSSSIQATQDNP
                     YGESKLQGEQLLREYAEEYGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQ
                     VNDRNVELTLNYVDDIVAEIKRAIEGTPTIENGVPTVPNVFKVTLGEIVDLLYKFKQS
                     RLDRTLPKLDNLFEKDLYSTYLSYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGKGVIRFRHVNDDEIIEYYVSGDKLEVVDIPVG
                     YTHNIENLGDTDMVTIMWVNEMFDPNQPDTYFLEV"
     gene            6185..7309
                     /gene="cap8G"
                     /locus_tag="P6L01_000765"
     CDS             6185..7309
                     /gene="cap8G"
                     /locus_tag="P6L01_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8G"
                     /protein_id="HDP3029850.1"
                     /translation="MEKLKLMTIVGTRPEIIRLSSTIKACDQYFNQILVHTGQNYDYT
                     LNQIFFDDLELRQPDHYLEAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCL
                     AAVSAKRLKIPVFHMEAGNRCFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGF
                     NKANIFVTGSPMTEVIEAHRDKINHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKS
                     LMNAINDIAKKYKMPVIYSTHPRSWKKIEESKFEFDPLVKQLKPFGFFDYNALQKDAF
                     VVLSDSGTLSEESSILKFPGVLIRTSTERPEVLDKGTVIVGGITYNNLIQSVELAREM
                     QNNNEPMIDAIDYKDTNVSTKVVKIIQSYKDIINRNTWRK"
     gene            7312..7938
                     /locus_tag="P6L01_000766"
     CDS             7312..7938
                     /locus_tag="P6L01_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HDP3029851.1"
                     /translation="MRIAIEKIIGLLKNQSSKESNVKIHRLAYITNSKFDGNNYIDRW
                     CKIRNSHIGEYSYIGFGSDFNNVEVGRYCSISSDVKIGLGKHPTHFFSSSPIFYSNNN
                     PFNIKQKFIDFNDQPSRTTIKNDVWIGANVIIMDGLTINTGAVIAAGSVVTKNVGAYE
                     VVGGVPAKVIKKRFDNKTIEKLLESKWWEKTPDKLKGFSVEYLNKKDT"
     gene            7943..9052
                     /locus_tag="P6L01_000767"
     CDS             7943..9052
                     /locus_tag="P6L01_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDP3029852.1"
                     /translation="MRILNIVSSNIVQDPRVLKQIETIKGVTNDYKIVGMNNSQATNR
                     RLENLDCNYRLLGSKVDPKNILSKLIKRIRFATGVIREIKAFKPDVIHANDFDVLLMV
                     YLSNYKKANIVYDAHEIYAKNAFINKVPLISKFVESIEKHIVKHRVNAFVTVSHAAKE
                     YYQSKGYKKEANVITNAPILNDSREFKEIENFKEIVYQGQIVMDRGYEEFIIASSAFK
                     QNAPSFIIRGFGPHEEVIKELISYNSENIRLDKPVEVKELVDKLAESNVGVILTKPVS
                     INFEYTVSNKIFECIHAGLPVILSPVKEHIYLNEKYKFGIVLKEVTPLEIEKAVRKLR
                     DNHDLFNHLRQNAIKASKILNWQIESERLVELYKF"
     gene            9066..10232
                     /locus_tag="P6L01_000768"
     CDS             9066..10232
                     /locus_tag="P6L01_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDP3029853.1"
                     /translation="MKFFVLCAIISMNIFIVISTFTKEVLGFPIEPVYYSTMVGIALI
                     TTVFAIYKIIVTQEIPRGLILLIAICLLYLAFYYFSPDKEEKLAKNNILFFLTWAVPA
                     AISGIYIKYINKATVERFFKLVFFIFSISFIFVILIPKLTGEIPSYINFGLMNYQNAS
                     YLSAFTAGLGIYFIMKGSVKHKWIYVLFTIIDIPIVFIPGGRGGAILLILYGLFAFIL
                     ITFKRGIPIAVKSIMYIFALSISSVLIYFLFTKGSNTRTFSYLQGGTLNLEGTSGRGP
                     IYEKGIYFIQQSPLLGYGPFNYYKLIGNIPHNIIIELILSFGLLGFFIIMICILLLVY
                     KMIRNYDPNTIDLLVMFIAIYPITLLMFSSNYLVVSEFWFVLFYFITKGRRHHG"
     gene            10225..11430
                     /locus_tag="P6L01_000769"
     CDS             10225..11430
                     /locus_tag="P6L01_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HDP3029854.1"
                     /translation="MVKKVFIMDSVKTIIGTLLIALGLQFLAYPIINQRVGNEAFGSI
                     LTIYTIITITSVVLGNTLNNIRLINMNLYKSNHYYWKFVSILLISILIESIALIIVFL
                     YFFNLNTIDIIFLILLNILMCLRIYLNVFFRMTLKYNQILYIALIQFLGLLIGLFLYY
                     LIQNWIVCFITSELFATIYTLVKLRGLTIGEYQSEDNNVVKDYVMLLSTNSLNNLNLY
                     LDRLILLPIIGGTAVTISFLSTFIGKMLATFLYPINNVVLSYISVNESDNIKKQYLKT
                     NLFAIAALCLVMIICYPITLIIVSLLYNIDSSLYSKFIILGNIGVLFNAVSIMIQTLN
                     TKHASITLQANYMTLHTITFIFITILMTIAFGLNGFFWTTLFSNIIKYVILNIIGLKS
                     KFINKKDVD"
     gene            11431..12636
                     /gene="cap8L"
                     /locus_tag="P6L01_000770"
     CDS             11431..12636
                     /gene="cap8L"
                     /locus_tag="P6L01_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8L"
                     /protein_id="HDP3029855.1"
                     /translation="MSEKKILILCQYFYPEYVSSATLPTQLAEDLIANHINVDVMCGW
                     PYEYSNHKQVSKTEMHRGIRIRRLKYSRFNNKSKVGRIINFFSLFSKFVINIPKMLKY
                     DQILVYSNPPILPLIPDVLHRLLKKKYSFVVYDIAPDNAIKTGATRPGSMIDKLMRYI
                     NRHVYKNAENVIVLGTEMKNYLLNHQISKNADNIHVIPNWYDMRQLQDNRIYNDTFKA
                     YREQYDKILLYSGNMGQLQDMETLISFLKLNKDQPQTLTILCGHGKKFADVKTAIEDH
                     RIENVKMFEFLTGTDYADVLKIADVCIASLIKEGVGLGVPSKNYGYLAAKKPLVLIMD
                     KQSDIVQHVEQYDAGIQIDNGDAHAIYNFINTHSSKELHEMGERAHQLFKDKYTREIN
                     TMKYYNLLK"
     gene            12647..13204
                     /gene="cap8M"
                     /locus_tag="P6L01_000771"
     CDS             12647..13204
                     /gene="cap8M"
                     /locus_tag="P6L01_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8M"
                     /protein_id="HDP3029856.1"
                     /translation="MKRLFDVVSSIYGLVVLSPILLITALLIKMESPGPAIFKQKRPT
                     INNELFNIYKFRSMKIDTPNVATDLMDSTSYITKTGKVIRKTSIDELPQLLNVLKGEM
                     SIVGPRPALYNQYELIEKRTKANVHTIRPGVTGLAQVMGRDDITDDQKVAYDHYYLTH
                     QSMMLDMYIIYKTIKNIVTSEGVHH"
     gene            13204..14091
                     /gene="capN"
                     /locus_tag="P6L01_000772"
     CDS             13204..14091
                     /gene="capN"
                     /locus_tag="P6L01_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     epimerase CapN"
                     /protein_id="HDP3029857.1"
                     /translation="MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSF
                     KDYDVLIHTAALVHNNSPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYG
                     KEGQVGKSDQIDTQTPMNPTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCP
                     GNFQRLMQLSKRLPIIPNINNQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTS
                     SVMYEIRRQSHRKTVLINMPSVLNKYFNKLSVFRKLFGNLTYSNTLYENNNALEVIPG
                     KMSLVIADIMDETTTKDKA"
     gene            14145..15407
                     /gene="cap8O"
                     /locus_tag="P6L01_000773"
     CDS             14145..15407
                     /gene="cap8O"
                     /locus_tag="P6L01_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8O"
                     /protein_id="HDP3029858.1"
                     /translation="MKLTVVGLGYIGLPTSIMFAKHGFDVLGVDINQQTIDKLQSGQI
                     SIEEPGLQEVYEEVLSSGKLKVSTTPEASDVFIIAVPTPNNDDQYRSCDISLVMRALD
                     SILPFLEKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDICLVHCPERVLPGKILE
                     ELVHNNRIIGGVTKACIEAGKYVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALA
                     NELTKICNNLNINVLDVIEMANKHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKL
                     IQTGREINNSMPAYVVDTTKQIIKALSGNKVTVFGLTYKGDVDDIRESPAFDIYELLN
                     QEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVI
                     FDTKNVVKSSFEDVSYYNYGNIFNFIDK"
     gene            15484..16629
                     /gene="cap8P"
                     /locus_tag="P6L01_000774"
     CDS             15484..16629
                     /gene="cap8P"
                     /locus_tag="P6L01_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8P"
                     /protein_id="HDP3029859.1"
                     /translation="MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDML
                     DSVLSIFDIQADHDLNIMQDQQTLADLTANALAKLDSIINEEQPDMILVHGDTTTTFV
                     GSLAAFYHQIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFE
                     NKDKERIFITGNTVIDALSTTVQNDFVSTIINKHKGKKVILLTAHRRENIGEPMHQIF
                     KAVRDLADEYKDVVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSY
                     LVLTDSGGIQEEAPTFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIED
                     DEAYQRMSQANNPYGDGQASRRICEAIEYYFGLRSDKPDEFVPLRHK"
     gene            complement(16694..17020)
                     /gene="isdI"
                     /locus_tag="P6L01_000775"
     CDS             complement(16694..17020)
                     /gene="isdI"
                     /locus_tag="P6L01_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdI"
                     /protein_id="HDP3029860.1"
                     /translation="MFMAENRLQLQKGSAEETIERFYNRQGIETIEGFQQMFVTKTLN
                     TEDTDEVKILTIWESEDSFNNWLNSDVFKEAHKNVRLKSDDDGQQSPILSNKVFKYDI
                     GYHYQK"
     gene            complement(17027..17410)
                     /locus_tag="P6L01_000776"
     CDS             complement(17027..17410)
                     /locus_tag="P6L01_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HDP3029861.1"
                     /translation="MRLILIVIGLIFTALGIAGAVLPLLPTTPFLLVAVFCFARSSDR
                     FYNWLINQKIYKEYVENFYLHRGYTLQQKIKILISLYIVIGFSIYMVDVLAVRVGLII
                     MVIIQTAVLFTFVKTLPKSNHKIEE"
     gene            17837..19324
                     /locus_tag="P6L01_000777"
     CDS             17837..19324
                     /locus_tag="P6L01_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDP3029862.1"
                     /translation="MAVNVRDYIAENYGLFINGEFVKGSSDETIEVTNPATGETLSHI
                     TRAKDKDVDHAVKVAQEAFESWSLTSKSERAQMLRDIGDKLMAQKDKIAMIETLNNGK
                     PIRETTAIDIPFAARHFHYFASVIETEEGTVNDIDKDTMSIVRHEPIGVVGAVVAWNF
                     PMLLAAWKIAPAIAAGNTIVIQPSSSTPLSLLEVAKIFQEVLPKGVVNILTGKGSESG
                     NAIFNHDGVDKLSFTGSTDVGYQVAEAAAKHLVPATLELGGKSANIILDDANLDLAVE
                     GIQLGILFNQGEVCSAGSRLLVHEKIYDQLVPRLQEAFSNIKVGDPQDEATQMGSQTG
                     KDQLDKIQSYIDAAKESDAQILAGGHRLTENGLDKGFFFEPTLIAVPDNHHKLAQEEI
                     FGPVLTVIKVKDDQEAIDIANDSEYGLAGGVFSQNITRALNIAKAVRTGRIWINTYNQ
                     VPEGAPFGGYKKSGIGRETYKGALSNYQQVKNIYIDTSNALKGLY"
     gene            19971..20930
                     /locus_tag="P6L01_000778"
     CDS             19971..20930
                     /locus_tag="P6L01_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDP3029863.1"
                     /translation="MNGKKANTINRYKYFHHVNHQKIQQSSKKTLWASLIITLLFTVI
                     EFVGGLVSNSLALLSDSFHMLSDVLALGLSMLAIYFASKKPTARYTFGYLRFEILVAF
                     LNGLALIVISIWILYEAIVRIIYPQPIESGIMFMIASIGLLVNIILTIILVRSLKQED
                     NINIQSALWHFMGDLLNSIGVIVAVVLIYFTGWRIIDPIISIVISLIILRGGYKITRN
                     AWLILMESVPQHLDTDQIMADIKNIDGILDVHEFHLWSITTEHYSLSAHVVLDKKYEG
                     DDYQAIDQVSSLLKEKYGIAHSTLQIENLQLNPLDEPYFDKLT"
BASE COUNT         7464 a         2918 c         3929 g         6653 t
ORIGIN      
        1 atatgtaaat actatatata taataccaat tttaatgaaa atttttaagg gaggtaaata
       61 atggaaagta cattagaatt aacaaaaatt aaagaagtat tacaaaaaaa cttgaagatt
      121 ttaattattt taccgctatt atttttaatt attagcgcta ttgttacatt tttcgtctta
      181 tcacctaaat atcaagctaa tactcaaatc ttagtgaatc aaactaaggg tgacaatcct
      241 cagtttatgg cacaagaggt tcaaagtaat attcaacttg taaatacgta taaagaaatt
      301 gttaaaagtc ctagaatttt agatgaggtg tcaaaggact taaatgataa gtattcacca
      361 tctaaattgt cgagtatgtt gacaattaca aaccaagaaa atacgcaact tatcaacatc
      421 caagttaaaa gtggtcataa acaagattcg gaaaaaattg cgaatagctt cgctaaagtt
      481 acaagtaaac aaattccgaa gattatgagt gtggataacg tatcaatttt atctaaagca
      541 gacggtacag cagttaaagt cgcaccaaaa actgtagtga atctaatcgg tgcattcttt
      601 ttaggattag ttgtcgcgct tatatatatc ttcttcaaag taattttcga taagcgaatt
      661 aaagatgaag aagatgtaga gaaagaatta ggattgcctg tattgggttc aattcaaaaa
      721 tttaattaag gatggttgct acttatgtca aaaaaggaaa atacgacaac aacactattt
      781 gtatatgaaa aaccaaaatc aacaattagt gaaaagtttc gaggtatacg ttcaaacatc
      841 atgttttcaa aagcaaatgg tgaagtaaag cgcttattgg ttacttctga aaagcctggt
      901 gcaggtaaaa gtacagttgt atcgaatgta gcgattactt atgcacaagc aggctataag
      961 acattagtta ttgatggcga tatgcgtaag ccaacacaaa actatatttt taatgagcaa
     1021 aataataatg gactatcaag cttaatcatt ggtcgaacga ctatgtcaga agcaattacg
     1081 tcgacagaaa ttgaaaattt agatttgcta acagctggcc ctgtacctcc aaatccatct
     1141 gagttaattg ggtctgaaag gttcaaagaa ttagttgatc tgtttaataa acgttacgac
     1201 attattattg tcgatacacc gccagttaat actgtgactg atgcacaact atatgcgcgt
     1261 gctattaaag atagtctgtt agtaattgat agtgaaaaaa atgataaaaa tgaagttaaa
     1321 aaagcaaaag cacttatgga aaaagcaggc agtaacattc taggtgtcat tttgaacaag
     1381 acaaaggtcg ataaatcttc tagttattat cactattatg gagatgaata agtatgattg
     1441 atattcataa ccatatattg cctaatatcg atgacggtcc gacaaatgaa acagagatga
     1501 tggatctttt aaaacaagcg acaacacaag gtgttacaga aatcattgta acatcacatc
     1561 acttacatcc tcgatatacc acacctatag aaaaagtgaa atcatgttta aaccatattg
     1621 aaagcttaga ggaagtacaa gcactaaatc taaagtttta ttatggtcag gaaataagaa
     1681 ttaccgatca aatccttaat gatattgatc gaaaagttat taccggtatt aatgattcac
     1741 gctatttact aatagaattt ccatcaaatg aagttccaca ctatactgat caattatttt
     1801 tcgaattaca gagtaaaggc tttgtaccga ttattgcaca tccagagcgg aataaagcaa
     1861 taagtcaaaa ccttgacata ctatacgatt taattaacaa aggtgcttta agtcaagtga
     1921 caacggcgtc attagcgggt atttccggta aaaaaattag aaaattagca attcaaatga
     1981 ttgaaaacaa tctgacacat ttcatcggtt cagatgcgca taacacagaa atcagaccgt
     2041 tcttaatgaa agacttattt aatgataaga aattacgtga ttattatgaa gatatgaacg
     2101 gatttattag taatgcgaag ttagttgttg atgataaaaa aattcctaaa cgaatgccac
     2161 aacaagatta taaacagaaa agatggtttg ggttataaac agcaaatgag gggttttatg
     2221 gcacatttat ctgtgaaatt gcggctttta atactagcat taatcgattc actgatagtg
     2281 acattttcag tattcgtaag ttattacatt ttagaaccgt atttcaaaac atattctgtc
     2341 aaattattaa tattggcagc tatatcacta ttcatatcgc atcatatttc agcatttatt
     2401 tttaatatgt atcatcgagc gtgggaatat gccagtgtga gtgaattgat tttaattgtt
     2461 aaagctgtga cgacatctat cgttattacg atggtggtcg tgacaattgt tacaggcaat
     2521 agaccgtttt ttagattgta tttaattact tggatgatgc acttgatttt aataggtggc
     2581 tcaaggttat tttggcgtat ttatcggaaa taccttggag gtaagtcatt taataagaag
     2641 ccaactttag ttgttggtgc tggtcaagca ggttcaatgc tgattagaca aatgttgaaa
     2701 agtgacgaaa tgaaacttga accggtatta gcagtcgatg atgacgaaca taaacgcaat
     2761 atcacaatta ctgagggtgt aaaagtccaa ggtaaaattg cggatattcc agaactagtg
     2821 aggaaatata agattaaaaa aatcatcatt gcaattccaa ctattggtca agagcgtttg
     2881 aaagaaatta ataatatttg ccatatggat ggcgttgagt tattgaaaat gccaaatata
     2941 gaagacgtca tgtctggtga gttagaagtg aatcaactga aaaaagttga agtagaagat
     3001 ttactaggca gagatcctgt tgaattagat atggatatga tatcaaatga attgacgaat
     3061 aaaactattt tagttacggg tgcaggtggt tcaataggat cagaaatttg tagacaagtt
     3121 tgtaatttct atccagaacg tattattcta cttggccatg gtgaaaacag tatttattta
     3181 atcaatcgtg aattgcgaaa tcgcttcgga aaaaatgttg atatcgttcc tattatagcg
     3241 gatgtgcaaa atagagcgcg tatgtttgaa attatggaaa cgtataaacc atacgcagtt
     3301 tatcatgcag cagcacacaa gcacgtgccg ttaatggaag acaaccctga agaagcagta
     3361 cataataata ttttaggtac gaaaaatact gctgaagctg ctaaaaatgc agaggtaaag
     3421 aaattcgtta tgatttctac ggataaagcc gttaatccgc ctaatgtcat gggagcttca
     3481 aagcgaattg cagaaatgat tattcaaagt ttaaatgatg aaacgcatcg aacaaatttt
     3541 gttgcagtga gatttggtaa tgtacttgga tcgagaggat ctgtgattcc acttttcaaa
     3601 agtcaaattg aagaaggtgg gccagttact gtgacacatc ctgaaatgac acgttacttt
     3661 atgacaattc ctgaagcttc tagactagtt ttgcaggcag gggcattagc agaaggtggc
     3721 gaagtatttg tgctagatat gggagaacca gtgaaaattg tagatttggc acgtaattta
     3781 attaagctaa gtggtaaaaa agaagacgac atacgcatta cttatacagg gattagaccc
     3841 ggcgaaaaaa tgtttgaaga gcttatgaat aaagatgagg ttcatcctga acaagtattt
     3901 gaaaaaattt atcgtggcaa agtacaacat atgaaatgta atgaagttga agcgattatt
     3961 caagacatcg tcaatgactt tagtaaagaa aaaattatta actatgccaa tggcaaaaag
     4021 ggagataatt atgttcgatg acaaaatttt attaattact gggggcacag gatcattcgg
     4081 taatgctgtt atgaaacggt ttttagattc taatattaaa gaaattcgta ttttttcacg
     4141 cgatgagaaa aaacaagatg acattcgaaa aaaatataat aattcaaaat taaagttcta
     4201 cattggtgat gtgcgtgata gtcaaagtgt agaaacagca atgcgagatg ttgattacgt
     4261 attccatgca gcagctttaa aacaagtgcc gtcatgtgaa ttctttccag ttgaggcagt
     4321 gaagacaaat attattggta cagaaaatgt cttacaaagt gctattcatc aaaatgttaa
     4381 aaaagtcata tgtttatcta cagataaggc agcgtatcct attaatgcta tgggtatttc
     4441 aaaagcaatg atggaaaaag tattcgtagc caaatcaaga aatattcgta gtgaacaaac
     4501 gcttatttgt ggtacaagat acggtaatgt gatggcttca agaggatcag taataccttt
     4561 gtttatcgac aaaatcaaag ctggagaacc tttaacgatt acagatcctg atatgacaag
     4621 atttttaatg agcttagaag atgcggtaga actagttgtt catgcattta agcatgcaga
     4681 gacaggagat attatggttc aaaaagcacc aagctcaacg gtaggggatc ttgcgaccgc
     4741 attattagaa ttgtttgaag ctgataatgc aattgaaatc attggtacgc gacatggaga
     4801 gaaaaaagca gaaacattgt tgacgagaga agaatacgca caatgtgaag atatgggtga
     4861 ttattttaga gtgccggcag actccagaga tttaaattat agtaattatg ttgaaaccgg
     4921 taacgaaaag attacgcaat cttatgaata taactccgat aatacacata ttttaacggt
     4981 ggaagagata aaagaaaaac ttttaacact agaatatgtt agaaacgaat tgaatgatta
     5041 taaagcttca atgagatagg agagattgac gttgaatatt gtaattacag gagcaaaagg
     5101 ttttgtagga aaaaacttga aagcagattt aacatcaacg acagatcatc atattttcga
     5161 agtacatcga caaactaaag aggaagaatt agagtcagca ttgttgaaag cagactttat
     5221 cgtgcattta gcgggtgtta atcgacctga acattacaaa gaattcagct taggaaacgt
     5281 gagttattta gatcatgtac ttgatatatt aactagaaat acgaaaaagc cagcgatatt
     5341 attatcgtct tcaatacaag caacacaaga taatccttat ggtgagagta agttgcaagg
     5401 ggaacagcta ttaagagagt atgccgaaga gtatggcaat acggtttata tttatcgctg
     5461 gccaaattta ttcggcaagt ggtgtaagcc gaattataac tcagtgatag caacattttg
     5521 ttacaaaatt gcacgtaacg aagagattca agttaatgat cggaatgttg aactaacgct
     5581 aaactacgtg gatgatatcg tcgctgaaat aaagcgtgct attgaaggaa ctccaacgat
     5641 tgaaaatggt gtacctacag taccaaacgt atttaaagtg acattgggag aaattgtaga
     5701 tttattatac aagttcaaac agtcacgtct cgatcgaaca ttgccgaaat tagataactt
     5761 gtttgaaaaa gatttgtata gtacgtattt aagctatcta cctagtacag actttagtta
     5821 tcccttactt atgaatgtgg atgatagggg ttcttttaca gaatttataa aaacaccgga
     5881 tcgtggtcaa gtttctgtaa atatttctaa accaggtatt actaaaggta atcactggca
     5941 ccatactaaa aacgaaaaat ttctagtcgt atcaggtaaa ggggtaattc gttttagaca
     6001 tgttaatgat gatgaaatca ttgaatatta cgtttctggc gataaattag aagttgtaga
     6061 cataccagta ggatacacac ataatattga aaatttaggc gacacagata tggtaactat
     6121 tatgtgggtg aatgaaatgt ttgatccaaa tcagccagat acgtatttct tggaggtata
     6181 gcgcatggaa aaactgaaat taatgacaat agttggtaca aggcctgaaa tcattcgttt
     6241 atcatcaacg attaaagcat gtgatcaata ttttaatcag atattagtac acactggtca
     6301 aaattatgat tatacattga atcaaatttt ctttgatgat ttggaattaa gacaaccgga
     6361 ccactactta gaggcagttg gaagtaacct tggagaaacg atggggaata ttattgcgaa
     6421 gacatatgac gttttattac gcgaacaacc agatgcactt ttaattcttg gtgatacaaa
     6481 tagttgttta gcagcagtat ctgctaaacg attaaagatt cctgtgttcc acatggaagc
     6541 gggtaataga tgctttgatc agaatgtacc tgaagaaatc aatcgtaaaa ttgttgacca
     6601 tgtcagtgat gtgaatctac cttatacaga acatagcaga cggtatttat tagatgaagg
     6661 cttcaataaa gcgaatatct ttgtgacagg atcaccgatg acagaagtga tagaagcgca
     6721 tcgagataaa attaatcaca gtgacgtttt aaataaacta ggattagaac cgcaacaata
     6781 cattttagta tctgcgcata gagaagagaa tatcgataat gaaaagaatt ttaaatcatt
     6841 aatgaatgcg ataaatgata ttgccaaaaa gtataaaatg cctgtgattt attcaacgca
     6901 tccaagaagt tggaagaaaa ttgaagaaag taaatttgaa tttgatccat tagttaaaca
     6961 gttaaagcca tttggtttct ttgattataa tgcattgcaa aaagatgcat ttgttgtgct
     7021 atcagatagt ggaacattgt cagaagagtc gtctattttg aagttccctg gtgtccttat
     7081 tcgaacttcc acagaaagac cggaagtact agataaaggt acggttattg taggtggtat
     7141 tacctataac aatctaatcc aatccgttga actagcaaga gagatgcaaa acaataacga
     7201 accgatgatt gatgctattg attataaaga cactaacgtt tcgacaaagg tagttaaaat
     7261 tattcaaagc tataaagata ttatcaatcg aaatacttgg aggaaatgac gatgaggata
     7321 gcgattgaaa agataattgg tttgctgaaa aaccagtcct ctaaagaatc gaatgttaag
     7381 attcatcgct tggcgtatat tacaaactca aaatttgatg gcaataacta tatagataga
     7441 tggtgtaaaa tcaggaattc tcacattggt gaatacagtt atattggatt tggtagtgat
     7501 tttaataatg tagaagtagg aagatattgt tcgatatctt cggatgtaaa aattgggtta
     7561 ggaaaacatc ctacacactt ttttagctca tcaccgattt tttattctaa taataatcca
     7621 tttaacataa agcaaaagtt tatagacttt aatgaccaac caagccgtac aacaattaaa
     7681 aatgatgtgt ggattggtgc aaatgtaatt attatggatg gattaacaat aaatactggt
     7741 gcagtcatag cagccggctc agttgttact aaaaatgtag gagcatatga ggttgttggt
     7801 ggggttcctg caaaagtgat taagaagcga tttgacaata aaacaattga aaaacttttg
     7861 gaaagcaagt ggtgggagaa aacgcctgac aaactaaaag gattttcggt tgaatattta
     7921 aataaaaagg atacttaatg atatgagaat tttaaatatt gtatcgagta atattgttca
     7981 agacccaagg gtacttaaac aaatagaaac aattaaaggc gttacgaatg attataaaat
     8041 tgttggaatg aataattcac aagctactaa taggcgattg gaaaatttag attgtaatta
     8101 tcgtttgtta ggtagcaagg tagatcccaa aaatattctt tctaaattaa ttaagcgtat
     8161 aagatttgca acaggtgtta tccgagaaat taaagctttt aaacctgacg tgattcatgc
     8221 aaatgatttc gacgtattat taatggtcta tttaagcaat tataaaaaag ctaatattgt
     8281 ttatgatgcg catgaaatat atgcgaaaaa tgcctttatt aataaagttc cacttatttc
     8341 aaagtttgta gaaagtatag aaaaacacat agtaaaacat cgtgttaatg ccttcgtaac
     8401 agtaagtcat gcagcaaaag aatattatca atctaaagga tataagaagg aagcgaatgt
     8461 tattacgaat gcacctattt taaatgatag cagagaattt aaagaaatcg aaaactttaa
     8521 agaaatcgta tatcaaggtc aaattgtaat ggacagagga tatgaagagt ttattattgc
     8581 ttcatcagct tttaaacaaa atgctccttc attcataatt cgagggtttg gtccgcatga
     8641 agaagtgata aaagaactga ttagttataa ctcggaaaat attaggttgg ataaaccagt
     8701 tgaagtaaaa gaattggttg ataagttagc agaaagtaat gttggtgtta tcttgacgaa
     8761 acctgtatct attaattttg aatatacagt atctaataaa atttttgaat gtatacatgc
     8821 tggtttacca gtaattttat ctcctgtcaa agagcatatt tatctcaatg aaaaatataa
     8881 atttggcatt gttttaaagg aagttacgcc gttagaaatt gaaaaggcgg ttagaaaatt
     8941 aagagataat cacgatttgt ttaatcattt acgtcaaaat gcaattaagg cgtctaaaat
     9001 tttgaattgg caaatagaaa gtgaacgatt agtagaatta tataaatttt aaagagaggt
     9061 aaactatgaa attttttgta ctttgtgcaa ttatcagcat gaacatattt atagtaatct
     9121 ctacatttac taaagaagta ttagggttcc ctatagagcc ggtgtattac tcaaccatgg
     9181 ttggtatagc attaattact acggtgtttg ctatttataa gataattgtc acgcaagaaa
     9241 ttccgcgagg gttaatatta ttaattgcta tatgtttgct ttatctagct ttttattatt
     9301 tttcaccaga taaggaagag aaactagcta aaaataatat tctattcttt ttaacatggg
     9361 cagttccagc ggcaattagt ggtatttata ttaaatatat aaacaaggct acggtagaaa
     9421 gattttttaa attagtattt ttcatatttt ctatttcatt tatttttgta attttaatac
     9481 caaaacttac aggtgagata cctagctata tcaattttgg acttatgaac tatcaaaacg
     9541 cttcgtacct ttcagcattt actgccggat taggcattta tttcattatg aaaggttcag
     9601 tgaaacataa gtggatatat gttctattta caataattga tatccctatt gtgtttatac
     9661 caggagggcg tggaggtgct attttattaa ttctttacgg cttatttgca tttatactta
     9721 ttacgtttaa aagaggaata cctattgcag taaaaagcat tatgtatatt tttgcattaa
     9781 gcatatctag tgtattgatt tactttcttt ttacaaaagg ttcgaatact agaacatttt
     9841 catatctaca aggtggaaca cttaatttag aaggtacttc tggaagagga ccgatttatg
     9901 aaaaaggtat ttactttatt caacaaagtc cgttattagg ctatgggcca tttaactatt
     9961 ataaactaat cggaaatata ccacataaca tcattattga gttgattcta tcatttggct
    10021 tattagggtt ttttatcata atgatttgca ttttgctact agtttataaa atgattagga
    10081 actatgatcc aaacactata gatttactcg ttatgtttat agcaatctat ccaatcacat
    10141 tattaatgtt tagttcaaat tatttagttg taagtgaatt ttggtttgtg ttgttctatt
    10201 ttattacaaa aggacggcgt catcatggtt aagaaagttt ttattatgga tagcgtaaag
    10261 acaataattg gtacgttgct tatagcttta ggattacaat ttttagctta tccaattatt
    10321 aatcaacgag taggtaatga agcgtttggt tctattttaa cgatttatac aataataaca
    10381 atcacgagtg ttgtattagg caatacgctt aacaatatac gattaattaa tatgaatcta
    10441 tacaaatcca atcattacta ctggaaattt gtgtcgatac ttttaatttc aattctgatt
    10501 gagagtatag ctttaattat tgtatttctt tactttttta atttgaacac catcgatatt
    10561 atctttttaa ttctacttaa tattttaatg tgtttaagga tttatctgaa tgtatttttt
    10621 aggatgactt taaaatataa tcagattttg tatattgctc ttattcaatt tttaggtttg
    10681 ctgataggac tatttctata ttatttaatc caaaactgga ttgtttgttt tattaccagt
    10741 gaattgtttg caacgatata tacattggtt aaattacggg gattaactat aggcgagtat
    10801 caaagtgaag ataataatgt ggtcaaagat tatgtgatgc tactgagtac aaatagcctt
    10861 aataatttga atctctactt agatagatta atcttattac caattatagg tggaacagct
    10921 gtaactatat catttctttc aacatttatt gggaaaatgt tagctacatt tctgtatccg
    10981 attaataatg tagtactttc atatatttct gtaaatgaaa gcgacaatat aaagaagcaa
    11041 tatttgaaaa ctaatctatt tgctatagct gcactatgtt tagtcatgat tatatgttat
    11101 ccaattacat taattattgt ctctttactg tataacattg attcaagttt atattcgaag
    11161 tttattattt taggtaatat aggtgtttta ttcaatgcag tgagtattat gatccaaact
    11221 ttaaatacaa aacacgcatc aataacatta caagcgaatt atatgacgct tcacacgatt
    11281 acatttatat tcataactat tttaatgaca attgcgtttg gtctaaatgg attcttttgg
    11341 acaacgctgt tcagcaacat tattaagtat gtgattttaa atattatagg tttaaagtct
    11401 aaattcatta ataaaaagga cgtcgattag atgagtgaaa aaaagatttt gattttatgt
    11461 cagtattttt atccggaata tgtatcttct gcgacgttac caactcaatt ggcggaagat
    11521 ttaattgcga atcacattaa tgtcgatgtc atgtgtggat ggccatatga atatagtaat
    11581 cataaacagg tttctaaaac cgagatgcat cgtggaattc gcattcgacg tctcaagtat
    11641 tcgaggttta ataacaaaag taaggttgga aggatcatca atttctttag tttattttca
    11701 aaattcgtga ttaatatacc taaaatgttg aaatatgatc agattcttgt ttactctaat
    11761 ccaccaatct tgccattaat accagacgtt ttacacagac tgcttaagaa aaaatattct
    11821 tttgtggtgt atgatatagc acctgataat gcgattaaga caggtgcaac tcgtccaggt
    11881 agcatgattg ataagctgat gcgttacatt aatagacatg tctacaagaa tgctgaaaat
    11941 gtcattgtcc ttggtacgga aatgaaaaac tacttactaa atcatcaaat ttctaaaaat
    12001 gctgacaata tccatgtgat tcctaactgg tatgacatgc gtcaattaca agacaatcgt
    12061 atctataatg acacatttaa agcttaccgt gagcaatacg acaaaatttt attgtatagc
    12121 ggtaatatgg ggcagttaca ggatatggag acacttatct catttttaaa attaaataag
    12181 gatcagcctc aaacgttaac aatactttgt ggtcatggta agaaatttgc agatgtcaaa
    12241 acggcaatag aagaccatcg tattgaaaat gttaaaatgt ttgagttttt aacaggtaca
    12301 gactatgctg acgtattaaa aattgcggat gtatgtattg catcgctgat taaagaaggc
    12361 gtcggtttag gcgttccgag caagaattat ggctaccttg cagctaagaa accgttggta
    12421 ctcatcatgg ataagcaatc tgatatcgtt caacatgttg aacaatatga tgcgggtatc
    12481 caaattgata atggcgatgc acatgccatt tataacttca tcaacactca ctcgagtaag
    12541 gaattgcacg agatgggtga gcgcgcacat caactgttta aagataaata tacgagagaa
    12601 attaatacta tgaagtatta caatctgttg aagtgaggag ataattatga agcgattatt
    12661 cgatgtagtg agttcaatat atggtttagt agttttaagt ccgattctgt taattacagc
    12721 attactaatt aaaatggaat cacctggacc agccattttc aaacaaaaaa gaccgacgat
    12781 taataatgaa ttgtttaata tttataagtt tagatcaatg aaaatagaca cacctaatgt
    12841 tgcaactgat ttaatggatt caacatcgta tataacaaag acagggaagg tcattcgtaa
    12901 gacctctatt gatgaattgc cacaattatt gaatgtttta aaaggagaaa tgtcaattgt
    12961 aggtcctaga ccagcgcttt ataatcaata cgaattaatc gaaaaacgta caaaagcgaa
    13021 cgtgcatacg attagaccag gtgtgacagg actagctcaa gtgatgggga gagatgatat
    13081 tactgatgat caaaaagtag cgtatgatca ttattactta acacatcaat ctatgatgct
    13141 tgatatgtat atcatatata aaacaattaa aaatatcgtt acttcagaag gtgtgcatca
    13201 ctaatgagaa aaaatatttt aattacaggc gtacatggat atatcggtaa tgctttaaaa
    13261 gataagctta ttgaacaagg acatcaagta gatcaaatta atgttaggaa tcaattatgg
    13321 aagtcgacct cgttcaaaga ttatgatgtt ttaattcata cagcagcttt ggttcacaac
    13381 aattcacctc aagcaaggct atctgattat atgcaagtga atatgttgct tacgaaacaa
    13441 ttggcacaaa aggctaaagc tgaagacgtt aaacaattta tttttatgag tactatggca
    13501 gtttatggaa aagaaggtca ggttggtaaa tcagatcaaa ttgatacaca aacaccaatg
    13561 aaccctacga ccaactatgg tatttccaaa aagttcgctg aacaagcatt acaagagttg
    13621 attagtgatt cgtttaaagt agcaattgtg agaccaccaa tgatttatgg tgcacattgc
    13681 ccaggaaatt tccaacggtt aatgcaattg tcaaagcgac tgccaatcat tcccaatatt
    13741 aacaatcagc gcagtgcatt atatattaaa catctgacag catttattga tcaattaata
    13801 tcattagaag tgacaggcgt gtatcatcct caagatagtt tttactttga tacatcgtca
    13861 gtaatgtatg aaatacgtcg ccaatcacat cgtaaaacgg tattgatcaa catgccttca
    13921 gtgttaaata agtattttaa taagttgtcg gtctttagaa aattattcgg caatttaaca
    13981 tacagcaata cgttatatga aaataataat gcacttgaag ttattcctgg aaaaatgtca
    14041 cttgttattg cggacatcat ggatgaaacg acaaccaaag ataaggcata agtcatctat
    14101 taaataaaat caacatacaa atcgttttat ttggaggtta tagtatgaag ttaacagtag
    14161 ttggcttagg ttatattggt ttaccaacat caattatgtt tgcaaagcat ggcttcgatg
    14221 tgcttggtgt tgatattaat cagcaaacga ttgataagtt acaaagtggt caaattagta
    14281 ttgaagaacc tgggttacaa gaggtttatg aagaggtact gtcatcggga aaattgaagg
    14341 tatctacaac gccagaagca tctgatgttt ttatcattgc cgttccgacg ccgaataatg
    14401 atgatcagta ccggtcatgt gacatttcgc tagttatgcg tgcattagat agtattttac
    14461 catttttaga aaaagggaat actattattg tagagtcgac aattgcgcct aaaacgatgg
    14521 atgattttgt aaaaccagtc attgaaaatt taggatttac aataggtgaa gatatttgtt
    14581 tagtgcattg tccagaacgt gtactgccag gaaaaatttt agaagaatta gttcataaca
    14641 atcgtatcat tggcggtgtg actaaagctt gtattgaagc gggtaaatat gtctatcgca
    14701 cattcgttca gggagaaatg attgaaacag atgcacgtac tgctgaaatg agtaagctaa
    14761 tggaaaacac atatagagac gtgaatattg ctttagctaa tgaattaaca aaaatttgca
    14821 ataacttaaa tattaatgta ttagatgtga ttgaaatggc aaacaaacat ccgcgtgtta
    14881 atatccatca acctggtcca ggtgtaggcg gtcattgttt agctgttgat ccgtacttta
    14941 ttattgctaa agaccctgaa aatgcaaagt taattcaaac tggacgtgaa attaataatt
    15001 caatgccggc ctatgttgtt gatacaacga agcaaatcat caaagcgttg agcgggaata
    15061 aagtcacagt atttggttta acttataaag gtgatgttga tgatataaga gaatcgccag
    15121 catttgatat ttatgagcta ttaaatcaag aaccagacat agaagtatgt gcttatgatc
    15181 cacatgttga attagatttt gtggaacatg atatgtcaca tgctgtcaaa gacgcatcgc
    15241 tagtattgat tttaagtgac cactcagaat ttaaaaattt atcggacagt cattttgata
    15301 aaatgaagca taaagtgatt tttgatacaa aaaatgttgt gaaatcatca tttgaagatg
    15361 tatcgtatta taattatggc aatatattta attttatcga caaataaaat gtgtcaaact
    15421 agggcataca tgattaagga aagataagct gtcatgtgtt tgaacttcag agaggataat
    15481 gttatgaaaa aaattatggt tattttcggt acgagacccg aagcaataaa aatggcacca
    15541 ttagtaaaag aaattgatca taatgggaac tttgaagcga acattgtgat tacagcacaa
    15601 catagagata tgttagatag tgtgttaagt atatttgata ttcaagctga tcatgattta
    15661 aatattatgc aagatcaaca aacgttagcg gaccttacgg cgaatgcgct tgctaaactt
    15721 gatagcatca ttaatgagga acagccggat atgattttag tacatggtga tactacaacg
    15781 acttttgtag gaagtttggc agcattttat catcaaattc cggttggaca tgtagaagct
    15841 ggacttcgaa cacatcagaa atactcacca tttcctgaag agttaaatcg agtcatggta
    15901 agtaatattg ctgaattgaa ttttgcgcca acagtaattg cagctaaaaa tttacttttt
    15961 gaaaacaaag acaaagagcg tatctttatt actggaaata cagttattga cgcattgtca
    16021 acaacagttc aaaatgattt tgtttcaacg attattaata aacataaagg caagaaagtt
    16081 attttactaa cagcgcatcg tcgtgaaaat attggggaac cgatgcatca gatttttaaa
    16141 gcagtaagag atttggcaga tgaatataaa gatgttgtct tcatttatcc aatgcatcgt
    16201 aatccaaagg taagagcgat tgccgaaaaa tatttatctg ggagaaatcg gattgaatta
    16261 attgagccat tagatgcgat tgagttccat aattttacaa atcaatcgta cctcgtgctg
    16321 acagattctg gtggtattca agaggaggct cctacatttg gaaaacctgt gttggtatta
    16381 aggaatcata cagagcgtcc cgaaggcgtt gaggcgggaa catcgagagt aattggcaca
    16441 gattatgaca atattgttcg aaatgtgaaa caattgattg aggatgatga agcgtatcaa
    16501 cgtatgagtc aagcgaataa tccatatggt gatggacaag catcacgacg tatttgtgaa
    16561 gcaatagaat attattttgg attgcgctca gacaagccgg atgaattcgt acctttacgt
    16621 cacaaataat aaaaaacccc taatcatgaa gttggtttag acaaccagcg gtgactaggg
    16681 gtttttaata tatttatttt tgatagtggt agccaatatc atatttgaat actttatttg
    16741 ataatattgg actttgctgt ccatcgtcat cactttttaa acgtacattt ttatgagctt
    16801 ctttaaatac atcggaattc aaccaattat taaagctatc ttcagattcc caaatagtta
    16861 agattttaac ttcgtctgta tcctcggtat ttaatgtttt agtgacaaac atttgttgga
    16921 agccttcaat agtttcaatg ccttgtctat tgtaaaaacg ttcaatcgtt tcttccgcac
    16981 tgcctttttg taattgtaat ctattttctg ccataaacat gggcaatcac tcctctattt
    17041 tatgatttga tttgggtaat gtttttacaa atgtaaagag tacagcggtt tgtatgataa
    17101 ccattatgat taatcctaca cggactgcaa gaacatccac catataaatt gaaaaaccta
    17161 ttacaatgta taagctaatt aaaattttaa ttttctgttg tagcgtgtag cctcgatgta
    17221 aataaaagtt ttctacatat tctttataaa ttttttgatt aataagccaa ttgtaaaacc
    17281 gatctgaact tcgagcaaaa caaaaaactg ctacgagtaa aaaaggggtc gttggcagta
    17341 aaggtaatac ggcacctgca ataccaagcg ctgtaaatat taagccaata acgattaaaa
    17401 taagtcgcat tgaaaaaact ccattctagt actaatgcgc atgtaatatt gttttagtaa
    17461 tataactcat gctaaatata atgtgtatga taagtgcaat gactcagtaa aatgaaacgt
    17521 tgttgaatta ttcttgtccc attaacgcat tttaagcgcg actttcataa caaccaaact
    17581 atttaatgag aattattctc aagtattata gttatattat gtgttttatt tttgaaaagt
    17641 gcaatatgtt ttcgaaaata agattatttt tatgtgcaaa aacgacgcaa aagttttaaa
    17701 aatgagactt ctgtgagctg attattttat aaaatgtaaa cgcttactat ataatgtgaa
    17761 tcatatcgtt taaaagcatt attaaatatg atgctaagag atttatatta tagccaataa
    17821 acaaaggaga gataatatgg cagtaaacgt tcgagattat attgcagaga attatggttt
    17881 atttatcaat ggggaatttg ttaaaggtag cagtgacgaa acaatcgaag tgactaatcc
    17941 agcaactgga gaaacactat cacatattac aagagcaaaa gataaagatg tcgatcatgc
    18001 agtcaaagtg gcgcaagagg catttgaatc atggtcatta acttctaaat cagaacgtgc
    18061 acaaatgttg cgtgatattg gtgataaatt aatggcacaa aaagataaaa ttgcaatgat
    18121 tgaaacatta aataatggta aaccgattcg tgagacaaca gcaattgata ttccatttgc
    18181 tgcaagacat ttccattatt tcgcaagtgt tattgaaaca gaagaaggta cagtaaatga
    18241 tatcgataaa gacacaatga gtatcgtacg acatgagccg attggcgtcg taggtgctgt
    18301 tgttgcttgg aacttcccaa tgctattagc tgcatggaag attgcgccag ccattgctgc
    18361 aggtaataca attgtgattc aaccttcgtc ttcaacacca ttaagtttat tggaagttgc
    18421 taaaattttc caagaggtat tacctaaagg tgttgtcaat atactaacgg gtaaaggttc
    18481 agaatcaggt aatgcaattt tcaatcatga tggtgtagat aaattatcat ttacgggctc
    18541 aactgatgta ggttatcaag ttgccgaagc tgcagcaaaa catctagtac ccgctacatt
    18601 agagcttggt ggtaaaagcg ccaatatcat attagatgat gctaatttag accttgcagt
    18661 tgaaggtatt cagttaggta ttttattcaa ccaaggtgaa gtatgtagtg caggttctcg
    18721 attattagtt catgaaaaaa tttatgatca attggtgcca cgtttacaag aggcattttc
    18781 aaatattaaa gttggagatc cacaagatga agctacacaa atgggtagtc aaactggtaa
    18841 ggatcaatta gataaaattc aatcatatat tgatgcagca aaagaatcag atgcacaaat
    18901 tttagcaggt ggtcatcgct taactgaaaa tggattagat aaagggttct tctttgagcc
    18961 gacattaatt gctgtgccag acaatcatca caaattagca caagaagaaa tatttggacc
    19021 agtgttaaca gtgattaaag tgaaggacga tcaagaagca attgatatag ctaatgattc
    19081 tgagtatggt ttagcaggcg gtgtattttc tcaaaatatc acacgtgcat taaatattgc
    19141 taaagctgta cgtacaggac gtatttggat taacacttac aaccaagtac cagaaggcgc
    19201 accatttggt ggttataaaa aatcaggtat cggtcgagaa acttataaag gtgcgttaag
    19261 taactatcaa caagttaaaa atatttatat tgatacaagc aatgctttaa aaggtttgta
    19321 ctagaataaa tatcgtttct gaagcgtgtt tgtaggtcag tctagcggta agtcttaaca
    19381 tttaacggcg ttgtttagat tttaagcaaa acaaaatata taggaacacg tatcatgata
    19441 ttaggatata atgactaaaa taatagcagt aggatggttt ttaattgcaa atcatcttac
    19501 tgctgttttt aattatgcta atttgcgatg cagctattat aaggacagag ttgtttatga
    19561 attgtggtga tttagaaata tgaagttcaa tatgcaaagt catcgtttgt tttaatatgt
    19621 ggaacaatca ttaaagttat tgtgattttt tgaacttaat gaaactaaac aataaatttg
    19681 agatactttt ttgtcatttt tatgtaacta acacaataat ctcgtacatt attaaaattt
    19741 tctatatgat agggataaag taaagagcga atgtgctgta aaagtttttc caaggtgata
    19801 ttacataaag gtataaaggg taaagattaa tgagttgtca tgtaaaggac gatgatgtat
    19861 aaatcatggt taattacgga agcattaata ttaatctgag aagctataaa gaattatttt
    19921 taaaagcgac aatattaaat acgacgcatt tatttaggag tggcaaacgt atgaatggga
    19981 aaaaggcaaa tacgataaac agatacaaat attttcatca tgtcaatcat caaaaaattc
    20041 aacaaagttc taaaaagacg ctgtgggcat cactaatcat cacattgtta tttacagtga
    20101 ttgaatttgt cggaggttta gtatctaatt cattggcatt actgtcagat tcatttcata
    20161 tgcttagtga tgtattagca cttggtttat ctatgttggc catttatttt gcaagtaaaa
    20221 agccgactgc acgatacaca tttggatatt taagatttga gatattagtt gcatttttaa
    20281 atggtttagc attaattgta atttcaatct ggattttata tgaagctatt gtacgtatta
    20341 tttatccgca accaattgaa agtggcatta tgtttatgat tgctagtatt ggtttactcg
    20401 ttaatattat tttgacaatt atccttgtaa ggtctttaaa acaagaagac aatatcaata
    20461 ttcaaagtgc attatggcat ttcatgggag acttattgaa ctctattggt gtcatcgttg
    20521 cagttgtatt gatttacttt acaggatggc gcatcatcga cccaatcatt agtattgtaa
    20581 tttcactcat cattttacgt ggtggttata aaattacgcg taatgcgtgg ttaattttaa
    20641 tggaaagtgt gcctcaacat ttggatactg atcaaattat ggcagatatt aaaaacatag
    20701 atggcatatt agatgtacat gaatttcatt tgtggagtat tacaacagag cattattcat
    20761 taagtgccca tgttgtgtta gataaaaaat atgagggtga tgattatcaa gcgattgatc
    20821 aagtatcatc attgttgaaa gaaaaatatg gcattgcaca ttcaacgttg caaattgaaa
    20881 acttgcaatt gaatccatta gatgagccat acttcgacaa attaacataa ataaaacatt
    20941 gtacaggctg ggacataaat caat
//
LOCUS       DAOBBH010000029        20300 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.029, whole genome shotgun sequence.
ACCESSION   DAOBBH010000029 DAOBBH010000000
VERSION     DAOBBH010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20300
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.029"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            364..855
                     /gene="lspA"
                     /locus_tag="P6L01_000779"
     CDS             364..855
                     /gene="lspA"
                     /locus_tag="P6L01_000779"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDP3029864.1"
                     /translation="MHKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHF
                     LNITSHRNNGAAWGILSGKMTFFFIITIIILIALVYFFIKDAQYNLFMQVAISLLFAG
                     ALGNFIDRILTGEVVDFIDTNIFGYDFPIFNIADSSLTIGVILIIIALLKDTSNKKEK
                     EVK"
     gene            855..1772
                     /locus_tag="P6L01_000780"
     CDS             855..1772
                     /locus_tag="P6L01_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDP3029865.1"
                     /translation="METYEFNITDKEQTGMRVDKLLPELNNDWSRNQIQDWIKAGLVV
                     ANDKVVKSNYKVKLNDHIVVTEKEVVEADILPENLNLDIYYEDDDVAVVYKPKGMVVH
                     PSPGHYTNTLVNGLMYQIKNLSGINGEIRPGIVHRIDMDTSGLLMVAKNDIAHRGLVE
                     QLMDKSVKRKYIALVHGNIPHDYGTIDAPIGRNKNDRQSMAVVDDGKEAVTHFNVLEH
                     FKDYTLVECQLETGRTHQIRVHMKYIGFPLVGDPKYGPKKTLDIGGQALHAGLIGFEH
                     PVTGEYIERHAELPQDFEDLLDTIRKRDA"
     gene            2172..2699
                     /gene="pyrR"
                     /locus_tag="P6L01_000781"
     CDS             2172..2699
                     /gene="pyrR"
                     /locus_tag="P6L01_000781"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDP3029866.1"
                     /translation="MSERIIMDDAAIQRTVTRIAHEILEYNKGTDNLILLGIKTRGEY
                     LANRIQDKIHQIEQQRIPTGTIDITYFRDDIEHMSSLTTKDAIDIDTDITDKVVIIID
                     DVLYTGRTVRASLDAILLNARPIKIGLAALVDRGHRELPIRADFVGKNIPTSKEETVS
                     VYLEEMDQRNAVIIK"
     gene            2917..4224
                     /locus_tag="P6L01_000782"
     CDS             2917..4224
                     /locus_tag="P6L01_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDP3029867.1"
                     /translation="MQNDEMFERTVKPVLDVNEKPQPAQWAFLSLQHLFAMFGATVLV
                     PFLTGLPISAALLASGIGTLLYILITKAQIPAYLGSSFAFITPIITGLSTHSLGDMLV
                     ALFMSGVMYVIIGILIKLSGTAWLMKLLPPVVVGPVIMVIGLSLAPTAVNMAMYENPG
                     DMKGYNISFLIVAMITLLVTIVVQGFFKGFLSLIPVLVGIIVGYVVAIFMGIVKFDAI
                     MSAKWIDFPHIYLPFKDYVPSFHLGLVLVMIPIVFVTVSEHIGHQMVLNKIVGRNFFE
                     KPGLDKSIIGDGVSTMFASIIGGPPSTTYGENIGVLAITRIYSIYVIGGAAVIAIVLA
                     FIGKFTALISSIPTPVMGGVSILLFGIIAASGLRMLVESKVDFANNRNLVIASVILVV
                     GIGNLVFNLKEIGINLQIEGMALAALSGIILNLILPKEKKQNN"
     gene            4252..5133
                     /locus_tag="P6L01_000783"
     CDS             4252..5133
                     /locus_tag="P6L01_000783"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDP3029868.1"
                     /translation="MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLF
                     FENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGESLYDTCKTLESIGCDLLVIRHP
                     FNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
                     VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGL
                     AEETRFAADDYHQKHGLNEVRYNKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQ
                     MENGVYLRMAVIDELLK"
     gene            5151..6425
                     /locus_tag="P6L01_000784"
     CDS             5151..6425
                     /locus_tag="P6L01_000784"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDP3029869.1"
                     /translation="MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGVDIID
                     AKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEH
                     FEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMM
                     YEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLL
                     AEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMN
                     PPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLY
                     THFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFL
                     SKADNTPFIGYKVYGNPILTMVEGEVKFEGDK"
     gene            6427..7527
                     /gene="carA"
                     /locus_tag="P6L01_000785"
     CDS             6427..7527
                     /gene="carA"
                     /locus_tag="P6L01_000785"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDP3029870.1"
                     /translation="MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETIS
                     DPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVL
                     ELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
                     TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVML
                     SNGPGNPEVVECAIPMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPV
                     KNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHP
                     EANPGPSDSNYLFDDFVAMMTNFKEKERHINA"
     gene            7520..10693
                     /gene="carB"
                     /locus_tag="P6L01_000786"
     CDS             7520..10693
                     /gene="carB"
                     /locus_tag="P6L01_000786"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDP3029871.1"
                     /translation="MPKRNDIKTILVIGSGPIIIGQAAEFDYAGTQACLALKEEGYRV
                     ILVNSNPATIMTDKEIADKVYIEPLTHDFIARIIRKEQPDALLPTLGGQTGLNMAIQL
                     HESGVLQDNNVQLLGTELTSIQQAEDREMFRTLMNDLNVPVPESDIVNTVEQAFKFKE
                     QVGYPLIVRPAFTMGGTGGGICHNDEELHEIVSNGLHYSPATQCLLEKSIAGFKEIEY
                     EVMRDKNDNAIVVCNMENIDPVGIHTGDSIVVAPSQTLSDVEYQMLRDVSLKVIRALG
                     IEGGCNVQLALDPHSFDYYIIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     LNPITGTSYAAFEPTLDYVISKIPRFPFDKFEKGERELGTQMKATGEVMAIGRTYEES
                     LLKAIRSLEYGVHHLGLPNGESFDLDYIKERISHQDDERLFFIGEAIRRGTTLEEIHN
                     MTQIDYFFLHKFQNIIDIEHQLKEHQGDLEYLKYAKDYGFSDKTIAHRFNMTEEEVYQ
                     LRMENDIKPVYKMVDTCAAEFESSTPYYYGTYETENESIVTDKEKILVLGSGPIRIGQ
                     GVEFDYATVHAVWAIQKAGYEAIIVNNNPETVSTDFSISDKLYFEPLTEEDVMNIINL
                     EKPKGVVVQFGGQTAINLADKLAKHGVKILGTSLENLNRAEDRKEFEALLRKINVPQP
                     QGKSATSPEEALANAAEIGYPVVVRPSYVLGGRAMEIVDNDKELENYMTQAVKASPEH
                     PVLVDRYLTGKEIEVDAICDGETVIIPGIMEHIERAGVHSGDSIAVYPPQTLTEDELA
                     TLEDYTIKLAKGLNIIGLINIQFVIAHDGVYVLEVNPRSSRTVPFLSKITDIPMAQLA
                     MRAIIGEKLTDMGYQEGVQPYAEGVFVKAPVFSFNKLKNVDITLGPEMKSTGEVMGKD
                     TTLEKALFKGLTGSGVEVKDHGTVLMTVSDKDKEEVVKLAQRLNEVGYKILATSGTAN
                     KLAEYDIPAEVVGKIGGENDLLTRIQNGDVQIVINTMTKGKEVERDGFQIRRTTVENG
                     IPCLTSLDTANALTNVIESMTFTMRQM"
     gene            10803..11495
                     /gene="pyrF"
                     /locus_tag="P6L01_000787"
     CDS             10803..11495
                     /gene="pyrF"
                     /locus_tag="P6L01_000787"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDP3029872.1"
                     /translation="MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQL
                     INEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLR
                     KHNQHTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLES
                     RMLTEKLGTSFLKVTPGIRPKGASQDDQHRITTPEEARQLGSTHIVVGRPITQSDNPV
                     ESYHKIKESWLV"
     gene            11495..12106
                     /locus_tag="P6L01_000788"
     CDS             11495..12106
                     /locus_tag="P6L01_000788"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDP3029873.1"
                     /translation="MAKEIAKSLLDIEAVTLSPNDLYTWSSGIKSPIYCDNRVTLGYP
                     LVRGAIRDGLINLIKEHFPEVEVISGTATAGIPHAAFIAEKLKLPMNYVRSSNKSHGK
                     QNQIEGAKSEGKKVVVIEDLISTGGSSVTAVEALKQAGAEVLGVVAIFTYGLKKADDT
                     FSNIQLPFYTLSDYNELIEVAENEGKISSEDIQTLVEWRDNLA"
     gene            12136..12348
                     /locus_tag="P6L01_000789"
     CDS             12136..12348
                     /locus_tag="P6L01_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029874.1"
                     /translation="MNDKTSNDLYGKIKHCNEFINHSNDSNLSSSHDVDESSTKQKHI
                     KNKTTIDHNDDLFKHVKDILRKQGQI"
     gene            12785..13186
                     /locus_tag="P6L01_000790"
     CDS             12785..13186
                     /locus_tag="P6L01_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDP3029875.1"
                     /translation="MDIPKITTFLMFNNKAEEAVKLYTSLFEDSEIITMAKYGENGPG
                     DHGTVQHSIFTLNGQVFMAIDANSGTELPMNPAISLFVTVKDTIEMERLFNGLKDEGA
                     ILMPKTNMPPYREFAWVQDKFGVSFQLALPE"
     gene            complement(13449..15146)
                     /locus_tag="P6L01_000791"
     CDS             complement(13449..15146)
                     /locus_tag="P6L01_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HDP3029876.1"
                     /translation="MAYDGLFTKKMVESLQFLTTGRVHKINQPDNDTILMVVRQNRQN
                     HQLLLSIHPNFSRLQLTTKKYDNPFNPPMFARVFRKHLEGGIIESIKQIGNDRRIEID
                     IKSKDEIGDTIYRTVILEIMGKHSNLILVDENRKIIEGFKHLTPNTNHYRTVMPGFNY
                     EAPPTQHKINPYDITGAEVLKYIDFNAGNIAKQLLNQFEGFSPLITNEIVSRRQFMTS
                     STLPEAFDEVMAETKLPPTPIFHKNHETGKEDFYFIKLNQFNDDTVTYDSLNDLLDRF
                     YDARGERERVKQRANDLVRFVQQQLHKYQNKLAKLIEEYEQSKNKDTEQLYGELITAN
                     IYRIKQGDKEVTALNYYTNEEVVIPLNPTKSPSANAQYYYKQYNRMKTRERELQHQIQ
                     LTKDNIDYFSTIEQQLHHISVHDIDEIRDELAEQGFMKQRKNQTKKKKAQIQLQHYVS
                     TDGDDIYVGKNNKQNDYLTNKKAKKTHTWLHTKDIPGSHVVIFNDAPSDTTIKEAAML
                     AGYFSKAGNSGQIPVDYTLIKNVHKPSGAKPGFVTYDNQKTLYATPDYELIQKMKQL"
     gene            15421..16044
                     /gene="gmk"
                     /locus_tag="P6L01_000792"
     CDS             15421..16044
                     /gene="gmk"
                     /locus_tag="P6L01_000792"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDP3029877.1"
                     /translation="MDNEKGLLIVLSGPSGVGKGTVRKRIFEDPSTSYKYSISMTTRQ
                     MREGEVDGVDYFFKTRDAFEALIKDDQFIEYAEYVGNYYGTPVQYVKDTMDEGHDVFL
                     EIEVEGAKQVRKKFPDALFIFLAPPSLDHLRERLVGRGTESNEKIQSRINEARKEVEM
                     MNLYDYVVVNDEVELAKNRIQCIVEAEHLKRERVEAKYRKMILEAKK"
     gene            16044..16262
                     /locus_tag="P6L01_000793"
     CDS             16044..16262
                     /locus_tag="P6L01_000793"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDP3029878.1"
                     /translation="MLNPPLNQLTSQIKSKYLIATTAAKRAREIDEQPETELLSEYHS
                     FKPVGRALEEIADGKISPVISSDYYGKE"
     gene            16478..17677
                     /gene="coaBC"
                     /locus_tag="P6L01_000794"
     CDS             16478..17677
                     /gene="coaBC"
                     /locus_tag="P6L01_000794"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDP3029879.1"
                     /translation="MKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVT
                     PLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDL
                     VTSTLLATETPKFIAPAMNVHMYENKRTQQNINILKEDGYHFIEPGSGFLACGYVAKG
                     RMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMG
                     YAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAA
                     AVSDYTPVDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENY
                     AQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILD
                     ELETRWQ"
     gene            17677..20085
                     /gene="priA"
                     /locus_tag="P6L01_000795"
     CDS             17677..20085
                     /gene="priA"
                     /locus_tag="P6L01_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDP3029880.1"
                     /translation="MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRT
                     IQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLE
                     VMLPSAIKAKYKKAFKMKDDIELPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
                     IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFL
                     TDIEDMGFSKSSLDGLIKKGYVEKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAI
                     KAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFK
                     RRFGDDVAVLHSGLSNGERYDEWQKIRDDRARVSVGARSSVFAPFKNLGLIIIDEEHE
                     STYKQEDYPRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKDVYHLLSLPNRVN
                     QQALPEIDIVDMREELSEGNRSMFSKDLREAIQLRLDRQEQVVLFLNRRGYASFMLCR
                     DCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGTGTQKVE
                     ELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITL
                     VGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQK
                     NDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKVMEASQHVHKILLQHLTEKAL
                     VLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDP
                     QMMM"
BASE COUNT         7180 a         2934 c         3831 g         6355 t
ORIGIN      
        1 ggacccatca tcaaaaattc tatttataga attttacagt aatgtgccag atgggcatag
       61 cgaagccatt caatacgaag tattgtataa atagagaaca gcagtaagat aatttttaat
      121 tagaaaatat cttactactg tctttttagg gatttatgtt ccagcctgct ttcctaattt
      181 ttaatgtcat cttaaatatg ataaatgaat aattaagttc atatttaatg tcaaaacata
      241 gtagtttatc aagtattgag tgagtaacat tagatttaat gtaatatgct tacttttttt
      301 attagcaggt gtaagctata atataaagag ttgtcttatg gacgattgat tggaggaacg
      361 aaaatgcaca aaaaatattt tattggcact tccattttaa tagcagtatt tgtcgttata
      421 tttgaccaag ttactaaata tattatagct actacaatga aaattggaga ttcatttgaa
      481 gtgataccgc actttttaaa cataacatca catcgaaata atggtgctgc atggggaata
      541 ttgagtggaa aaatgacatt tttctttatt attaccatta ttatattaat agccttagta
      601 tatttcttta ttaaagatgc tcaatataat ttgtttatgc aagttgctat tagtttactt
      661 tttgcaggtg cacttggaaa ctttattgat agaattttaa caggagaagt tgttgacttt
      721 attgatacaa atatttttgg ttatgatttt ccaatattta atatagcaga ttcaagttta
      781 acaattggtg taatattaat tattattgcc ttattaaagg atacttccaa taaaaaggag
      841 aaggaggtta agtaatggag acttatgaat ttaacattac agataaagaa caaacaggta
      901 tgcgtgtaga taagttgctg cctgaattaa ataatgattg gtctcgtaac cagatacaag
      961 attggattaa agcaggttta gtcgttgcaa acgataaagt tgttaaatct aattataaag
     1021 tgaaacttaa tgatcatata gttgtcactg aaaaagaagt ggttgaagct gatattctac
     1081 ctgaaaattt aaatttagat atttattatg aagatgacga tgttgcagtt gtatataaac
     1141 cgaaaggcat ggtagttcat ccatcaccag ggcattatac caatacatta gttaatggtt
     1201 taatgtatca aattaaaaat ttgtcaggta ttaatggaga aattcgtcca ggtattgttc
     1261 accgtataga tatggatact tctggtttat taatggttgc taaaaatgat attgctcatc
     1321 gtgggcttgt agaacaatta atggataaat ctgttaaaag aaaatatatc gctttagttc
     1381 acgggaatat tcctcatgat tacggtacaa tcgatgcgcc aattggtaga aacaaaaatg
     1441 atcgtcaatc tatggctgtt gttgatgatg gtaaggaagc agtgacacat tttaacgtac
     1501 tagaacattt taaagattat acgcttgttg aatgtcaact tgaaacagga cgtacgcatc
     1561 aaatccgtgt gcacatgaaa tatattggct tcccattagt tggtgatcca aagtatggac
     1621 cgaaaaagac attggatatt ggtggtcaag ctctacatgc tggacttatt ggattcgaac
     1681 atccagtaac aggtgaatat attgaaagac atgctgaatt accacaagac tttgaagatt
     1741 tattagatac aattcgaaaa agagatgcat aattgtgttg tgctataatt acgataacat
     1801 tattatgtaa actataagta tttatcgttt tcgttttttt aaaatgaaaa cacattataa
     1861 aatagattaa ggttaacatt tttactgaga tcggttaatt tttcaatata gattgacaaa
     1921 gcgcctgatt agtattatcc ttataacagt ttaagtacaa cataaagagt ctttaattga
     1981 gcgtccagag agacgtcaaa gacatgaaaa tatagatata tactaaaatg atcataacac
     2041 tttcacatat gcaagaataa gtagtaatac tacgattctt ctatgtaaat aggctgattg
     2101 tatatactaa acctcatgtc gatattaaag acatgaggtt ttttgtttct aaattttgaa
     2161 agggtgagaa tatgtctgaa cgtattataa tggatgatgc cgcaatacaa cgtacagtga
     2221 cgagaatcgc tcacgaaatt ttggagtata acaaaggtac tgataattta attcttttag
     2281 gtatcaaaac aagaggtgaa tatttagcga atcgtataca agataaaatt catcaaattg
     2341 agcaacaacg tatacctact ggaacaattg atattacata ctttagagat gatatagagc
     2401 acatgtcatc acttacgaca aaagacgcaa tagacatcga cacagatatt acagataaag
     2461 tagtcatcat tattgatgat gtgctgtata ctggtcgaac ggttcgtgct tcacttgatg
     2521 ctattttgct aaatgctaga cctattaaaa ttggtttagc tgctttggtt gatcgaggac
     2581 atcgtgagtt accaattcga gcagattttg ttggtaaaaa tatacctact tctaaagagg
     2641 aaacggtaag tgtctattta gaagaaatgg atcaaagaaa tgcagttata attaaataat
     2701 acccctttta attcagtacg agagactgaa aaaggtgggg gagttgaatg ataaataagt
     2761 tttcatatgc ttgtttatct aataataccc tcatgttcga caactataag tcaatttcta
     2821 tggacgaaaa tgacttgtag caacttgtca cactttttgt ctctttacat atactgatgt
     2881 aaagagattt ttttattatc ggaggaaatg aatattatgc aaaatgatga aatgtttgaa
     2941 cgaacagtaa aaccagtact agatgtaaat gaaaaaccac aaccagcgca atgggcattt
     3001 ttaagcttac aacatttatt tgcgatgttt ggcgcaacag tactagtacc attcttaaca
     3061 ggactaccaa tatccgcagc gttactagct tcgggaatcg gtacattact ttatatctta
     3121 ataacgaagg cgcaaatacc agcatacttg ggatctagct ttgcatttat cacaccaatt
     3181 atcacgggat taagtacgca tagcttagga gacatgcttg tagcattatt catgagtggt
     3241 gttatgtacg tcatcatcgg aattctaatc aaattaagtg ggacagcatg gttaatgaaa
     3301 ttattaccac cagttgttgt tggaccagtc atcatggtaa ttggtttaag cttagcgcct
     3361 actgcagtca atatggcaat gtatgaaaat cctggagata tgaaaggtta caatatcagt
     3421 ttcttaattg ttgcaatgat aaccttgctt gtaacaatag tcgttcaagg attttttaaa
     3481 ggattcctat ctttaattcc agtacttgta ggtattatcg taggttatgt ggtagcgatt
     3541 tttatgggga ttgtgaaatt tgatgcaatt atgtcagcaa aatggataga tttccctcat
     3601 atttatctgc catttaaaga ttatgtacct tcatttcact taggacttgt acttgtaatg
     3661 attccgattg tgtttgtaac agtaagtgaa catattgggc accaaatggt attgaataaa
     3721 atcgtaggta gaaacttctt tgaaaagcca ggacttgata aatcaatcat tggtgatggt
     3781 gtttctacaa tgtttgccag tattattggt ggaccaccaa gtacaacata cggtgaaaat
     3841 atcggtgtat tagcgattac aagaatatac agtatttacg tcattggtgg tgcagcagtt
     3901 atagcaattg ttttagcatt cattggtaag ttcactgcat taatttcttc tatacctaca
     3961 ccagttatgg gaggagtatc tatattactt ttcggtatta ttgcagcaag tggcttaaga
     4021 atgttagttg aaagcaaagt agattttgcg aacaatcgaa atttagttat agcttctgta
     4081 attttagttg taggtatcgg taatttagta tttaacttaa aagaaattgg tatcaacctt
     4141 caaattgagg ggatggcatt agctgcactt tcaggaatta ttttgaactt aatcttacct
     4201 aaagagaaaa aacaaaacaa ttaagattta caaattaagg agggcgcttt tatgaatcat
     4261 ttattatcaa tggaacattt atctacagat caaatataca aacttatcca aaaggcaagt
     4321 caatttaaat ctggtgaacg tcaactacca aactttgaag ggaaatatgt cgcaaattta
     4381 ttctttgaaa attctactcg aacaaaatgt agttttgaaa tggcagaact taagctaggg
     4441 ttaaaaacga ttagctttga aacatcaaca tcatctgttt caaaaggtga atctttatat
     4501 gacacatgta aaactttaga aagtattggc tgtgatttat tagtcattag acatccgttt
     4561 aataactact atgaaaaatt agcgaatatt aacatcccaa ttgcgaatgc tggtgatggt
     4621 agtggacaac atccaacaca aagtttactt gatttaatga cgatatatga agaatatgga
     4681 tattttgaag gcttgaatgt attgatttgt ggagacatta aaaattcacg tgtcgcacgt
     4741 agtaattacc atagtttaaa agcattaggt gcaaacgtaa tgtttaatag cccaaatgct
     4801 tggattgatg attctttaga agcaccttat gtaaatatag atgatgttat agaaacagta
     4861 gatatagtta tgttattaag aattcaacat gaaagacatg ggcttgcaga agaaactaga
     4921 tttgcagcag atgattatca tcaaaagcat ggcttaaatg aagtgcgcta taacaaatta
     4981 caagaacatg ctattgttat gcatccggca cctgtgaata gaggagtaga aatacaaagc
     5041 gatttagtag aagcttcaaa atcaagaatt tttaagcaaa tggaaaatgg cgtttactta
     5101 agaatggcag tcattgatga attattaaaa taggtaaggg gacgaaaatg atgaaattaa
     5161 ttaaaaacgg taaagtatta caaaatggag aattacaaca agcagatatt ttaattgatg
     5221 gtaaggtaat taaacaaatt gcacctgcaa ttgaaccaag caatggtgtt gacatcatag
     5281 atgcgaaagg tcactttgtg tcacctggat ttgtcgatgt tcatgttcat ttacgtgaac
     5341 ctggtggtga atataaagag acaattgaaa ctggtactaa agctgctgct agaggcggat
     5401 ttacaactgt atgtccaatg cctaacacaa gaccggtacc agattctgta gaacattttg
     5461 aagctttaca aaaattaatc gatgacaatg ctcaagtacg tgtattacct tatgcttcaa
     5521 ttacaacacg tcaattaggt aaagaattgg ttgatttccc agcactagta aaagaaggtg
     5581 cctttgcgtt tacagatgac ggtgtaggag tacaaactgc aagcatgatg tatgaaggca
     5641 tgattgaagc tgcaaaagta aacaaagcca tcgtagcaca ctgtgaagat aattcattaa
     5701 tctatggtgg tgcaatgcat gaagggaaac gcagtaaaga gttaggtata ccaggtattc
     5761 caaacatttg tgaatctgtt caaatcgcaa gagatgtact attagctgaa gcagcaggtt
     5821 gtcattatca tgtatgtcat gtttctacta aagaaagtgt tagagtcatt cgtgacgcta
     5881 aacgcgcagg cattcatgtt acagctgaag ttacaccaca ccatttattg ttaacagaag
     5941 atgatattcc tggtaataat gccatttata aaatgaatcc accattgaga agtactgaag
     6001 atagagaggc tttgttagaa gggttactag acggtacaat tgactgtatc gcaacagacc
     6061 atgcaccaca tgcacgtgat gaaaaagcac aaccaatgga aaaagcacca ttcggaattg
     6121 ttggtagtga aacagcattc ccattattat atacgcattt tgtaaaaaat ggtgattgga
     6181 cattacaaca attagtagat tacttaacaa ttaaaccatg tgagacattt aatttagaat
     6241 acggcacatt aaaagaaaat ggttatgcag atttaacaat cattgattta gatagtgaac
     6301 aagaaattaa aggagaagat ttcttatcaa aagcagataa tacaccattt atcggctata
     6361 aagtttatgg aaatccgatc ttaacaatgg ttgaaggcga agttaaattt gagggggata
     6421 aataatatgc aaagcaaacg ttatctagtg ttagaagacg gttcttttta cgaaggctac
     6481 cgtttaggat ctgataactt aactgtagga gaaattgtat ttaatacagc tatgacaggt
     6541 tatcaagaaa ctatttcaga tccatcatat acaggtcaga tcattacttt tacgtatcca
     6601 ttaatcggta attatggtat caatagagac gattttgaat cattagtacc tacattaaac
     6661 ggtattgtag tgaaagaagc gagtgcgcat ccaagtaatt ttagacaaca aaagacactt
     6721 catgacgttt tagaattgca tcaaattcca gggattgcag gtgttgatac aagaagtatt
     6781 acgcgtaaaa ttcgacaaca cggtgtgtta aaagctggtt ttactgatcg aaaagaagat
     6841 attgatcaac ttgtcaaaca tttacaacaa gtagaattac ctaaaaacga agtagaaatc
     6901 gtttcgacta aaacaccgta tgtttcgaca ggtaaggatc taagtgtcgt acttgtagac
     6961 tttggtaaga agcaaaatat tgttcgagaa ttaaacgtca gaggttgtaa cgtcacagtt
     7021 gtaccatata caactactgc cgaagaaatt ttagcaatgg ctccagatgg cgttatgcta
     7081 tcaaacggac caggtaatcc tgaagttgta gaatgtgcga ttccaatgat tcaaggaatt
     7141 ttagggaaaa ttccgttctt tggtatctgt ctaggacatc aactttttgc attatctcaa
     7201 ggagcaagct catttaaaat gaagtttggt catcgtggtg cgaaccatcc agttaaaaat
     7261 ttagagactg gaaaagttga tattacgagt caaaaccatg gatatgcaat agatatagat
     7321 tcgttaaaaa gtactgattt agaagttact catcttgcat taaatgatgg tactgtagaa
     7381 ggtttaaaac ataaaacatt accagcattt tctgttcaat accatcctga agcaaatcca
     7441 ggaccgtcag attcaaacta tctatttgat gattttgtag caatgatgac taattttaag
     7501 gaaaaggagc gtcatatcaa tgcctaaacg taatgatatc aaaacaattt tagtaatagg
     7561 gtctgggcca attatcatag gtcaagcagc tgaatttgat tatgctggaa cacaagcatg
     7621 tctagcttta aaagaagagg gatatcgagt tattcttgta aattcaaatc cagcgacaat
     7681 catgactgat aaggaaattg cggataaagt atatatcgaa ccgttaactc atgattttat
     7741 agcgcgaatt atacgtaaag agcaacctga cgctttactt ccaactttag gtggtcaaac
     7801 aggtttaaac atggcgattc aactacacga aagtggtgtg cttcaagata ataacgtcca
     7861 attattagga actgagctaa catcaattca acaagcagaa gaccgtgaaa tgtttagaac
     7921 attaatgaat gatttaaacg ttcctgtacc agagagtgac attgtaaata cagtagagca
     7981 agcctttaaa ttcaaagagc aagtgggata cccgctaatt gttagaccgg catttacgat
     8041 gggtggtacc ggaggcggta tttgtcataa tgatgaagaa ttacatgaaa tcgtctcaaa
     8101 tggtcttcat tatagtccag caacgcaatg tttattagaa aaatctatcg caggttttaa
     8161 agaaatcgaa tacgaagtaa tgcgtgataa aaacgataat gccatcgttg tatgtaacat
     8221 ggaaaatatt gatccagttg gtattcatac tggcgattca attgttgtgg ctcctagtca
     8281 aacattatca gatgttgagt atcaaatgtt acgtgatgtt tcattaaaag ttattcgagc
     8341 tttaggtatc gaaggtggtt gtaatgttca attagcatta gatccccatt cattcgatta
     8401 ttatattata gaagtaaatc cgcgtgtatc acgttcatca gcgttagctt caaaagcaac
     8461 aggatatcct attgcaaaat tagctgctaa aatcgcggtt ggtctaacat tagatgaaat
     8521 gttaaatcca attacaggaa catcttatgc agcgtttgaa ccaactttag actatgtgat
     8581 ttcaaaaata ccaagatttc cttttgataa atttgaaaaa ggagaacgag agcttggcac
     8641 acaaatgaaa gcaacaggtg aagttatggc cattggtcga acttacgaag aatcattgtt
     8701 aaaagcaatt cgatcacttg agtatggtgt gcatcactta ggattaccaa atggtgaaag
     8761 cttcgatctt gattatatta aagaacgtat ttcacaccaa gatgatgaac gattattttt
     8821 catcggcgaa gcaattagaa gagggacaac attagaagaa attcataata tgactcagat
     8881 tgattacttc ttcttacaca agttccaaaa cattattgat attgagcatc aattaaaaga
     8941 gcatcaaggt gatttagaat atcttaaata tgcaaaagat tatggattta gtgataaaac
     9001 aatagcgcat cgctttaata tgacggaaga agaagtatat caattgcgta tggaaaatga
     9061 tattaaacct gtttacaaga tggttgatac ttgcgcagct gaatttgaat cttcaacacc
     9121 atattattat ggtacatacg aaactgaaaa tgaatccata gttactgaca aagaaaaaat
     9181 cttagtatta ggctctggac caattcgaat cggccaaggt gtagaatttg actatgcgac
     9241 agttcacgcc gtttgggcaa ttcaaaaagc agggtacgaa gcgataattg tgaataacaa
     9301 tccagaaaca gtttcaacag acttctcaat ttctgacaaa ttatactttg aacctttaac
     9361 tgaagaagat gtgatgaata tcattaattt agaaaaacct aaaggtgtcg ttgtacaatt
     9421 tggaggacaa acagcgatta atttagcaga caaattggct aaacatggtg ttaaaatact
     9481 tggtacttca ctagaaaatc taaatcgtgc tgaagataga aaagaatttg aagcactatt
     9541 aagaaaaatt aacgtgccac agccacaagg gaaatcagct acatcacctg aggaagcatt
     9601 agcgaatgct gcagaaatcg gatatccggt tgtagtaaga ccttcttatg tattaggtgg
     9661 tcgcgcaatg gaaattgtag acaatgacaa agagttagaa aactatatga cccaggctgt
     9721 aaaagcgagt ccggaacatc cggtactagt cgatagatat ttaactggta aagaaattga
     9781 agttgatgcg atttgtgatg gagaaacggt cattattcca ggaatcatgg aacatattga
     9841 acgtgctggt gtacatagtg gtgactcaat cgctgtatat ccgccacaaa ctttgacaga
     9901 agacgagtta gcaacacttg aggactatac tataaaatta gctaaaggtt taaacatcat
     9961 tggcttaatc aacattcaat tcgttatagc tcacgatggt gtatatgttt tagaagtaaa
    10021 cccacgttct agtagaacgg taccattctt aagtaaaatt actgatattc caatggcaca
    10081 attagctatg cgagcaatca ttggggaaaa actaacagat atgggttatc aagaaggggt
    10141 tcaaccatat gctgagggtg tatttgtgaa agcaccggta tttagtttta ataaattgaa
    10201 aaatgttgat attactttag gacctgaaat gaagtctaca ggtgaagtaa tggggaaaga
    10261 tactacatta gaaaaggcgt tattcaaagg gttaacaggt agtggtgttg aagttaaaga
    10321 tcacggtaca gtattaatga ccgtcagtga caaagataaa gaggaagttg ttaaattggc
    10381 acaacgctta aatgaagttg gctataaaat tttagcaacg tctggaacag ctaataaatt
    10441 agctgagtat gacatacctg cagaagtagt aggcaaaatc ggtggcgaaa atgatttatt
    10501 aacacgtatt caaaatggtg atgttcaaat cgttataaat acaatgacta aaggtaaaga
    10561 agtagaaagg gatggcttcc aaattagacg tactacagtt gaaaatggta ttccatgttt
    10621 gacatcttta gatacagcta atgccttaac gaatgtaatt gaaagtatga catttacaat
    10681 gcgtcaaatg taaatcaatc aaactgtatc ggtggggctg taattaacca tttacttaaa
    10741 gaagtttata ttacagcctc attattttaa tgaatttctt aatataaagg gagacatata
    10801 tgatgaaaga tttaccaatt attgcattag attttgaatc aaaagaaaaa gtaaatcaat
    10861 ttttagattt atttgatgaa tcattattcg taaaagtagg tatggaactt ttttatcaag
    10921 aaggtcctca attaattaat gagataaaag aaagaggcca tgatgtattt ttagatttaa
    10981 aactgcatga tattcctaat acagttggta aggcgatgga aggactagct aaattgaatg
    11041 ttgatctggt aaatgttcat gctgctggtg gcgtaaaaat gatgtctgag gccattaaag
    11101 gattaagaaa acataatcaa catacaaaaa ttattgcagt aacacagctt acgtcaacaa
    11161 cagaagacat gttacgacac gaacaaaata tacaaacatc gattgaagag gccgttttaa
    11221 attatgccaa gttagcaaat gcagctggtt tagatggcgt tgtttgttca cctcttgaaa
    11281 gtcgtatgtt gactgaaaag ttaggtacat catttttaaa agtaacacca ggtattagac
    11341 ctaaaggtgc atctcaagat gaccaacacc gtattacgac accggaagaa gcaagacagc
    11401 ttggttcgac gcatattgta gtcggtagac cgattacaca aagtgacaat ccagtcgaaa
    11461 gttatcataa aattaaagaa agttggttag tataatggct aaagaaattg caaaatcatt
    11521 attagatatt gaagctgtaa cattatcacc aaatgattta tatacatgga gttcaggtat
    11581 taaatcaccg atttactgtg ataaccgtgt tacgttaggt tatcctttag ttcgaggcgc
    11641 aatccgcgat ggtttaatta acttaattaa agaacacttt cctgaagtag aagttatttc
    11701 tggtactgca acagctggta ttccacatgc agcttttatt gctgaaaaat taaaattacc
    11761 aatgaattat gttcgttcat caaataagag tcatggtaag caaaatcaaa tcgaaggtgc
    11821 taaaagtgaa ggtaaaaaag tagttgtgat agaagattta atttcgacag ggggatcttc
    11881 agtcacagca gttgaagcct taaaacaagc aggtgcagaa gtattaggtg ttgtagctat
    11941 ctttacttac ggtttgaaaa aagcagatga tacatttagc aatattcaac taccttttta
    12001 cactttaagt gattacaatg aattaattga agtagctgaa aatgaaggta aaatttctag
    12061 tgaagatatc caaacattag ttgaatggag agacaactta gcataatata gacactagaa
    12121 ggaggaattc aacaaatgaa tgacaaaaca tctaatgatt tatatgggaa gataaaacat
    12181 tgtaacgaat ttatcaatca ttcaaatgat tccaatctat ctagtagtca cgatgtcgac
    12241 gaaagttcaa cgaagcaaaa acatataaaa aataaaacaa ctatagatca taatgatgat
    12301 ttatttaaac atgtaaagga tatattacgt aaacaaggac aaatttaaaa acaaaaaagc
    12361 tatagtgttt aatatcaggg gctgcttaac cgcatccgaa tattacaaac actatagctt
    12421 ttattatata aaaaatttat tagcggataa ttaactttat taaaatccaa ccaattaaga
    12481 atacgagtaa taggactaat actggaatca cataatgtaa cataacgtcc ctcctttaac
    12541 ttaattttaa ttgtaatcaa atttgacaat aagtcaaaac attaatacct atgataagta
    12601 tcatttatta acatatgtat catattttta atcttgcgta atttttatcg ttaactatgt
    12661 gatttaatca acaggccaat aagacgtttg tcattcgtgc acataacggt atgacgcgct
    12721 tttgtcattt taattatgtt aagataatag tagttataga agttcgatct ataggaggca
    12781 tagcatggat attccaaaaa tcacgacatt tttaatgttt aataacaaag ctgaagaagc
    12841 tgttaaacta tacacaagct tatttgaaga tagtgagatt ataacaatgg ctaagtatgg
    12901 tgaaaatgga cctggtgatc acgggactgt acaacactca atatttacat taaatggaca
    12961 agtattcatg gcgattgatg ctaatagtgg cacagaatta ccaatgaatc ctgcgatttc
    13021 attatttgtt acagtaaaag atactattga aatggaacga ctatttaatg gattaaaaga
    13081 tgaaggtgcc attttaatgc caaaaacgaa tatgccacca tacagagagt ttgcttgggt
    13141 tcaagataag tttggagtaa gttttcaatt agcattacct gagtaaaagg attcgcacag
    13201 cgttgaatga taaagaaaca ctttttctta tgcatgcgtt ttacctatgt atttctattt
    13261 taagtacaca ttagcatttt agtttcactc atttttaaaa tcacaaagat aatttgtaac
    13321 ttaaatgatg atacgttatt taaaaacacg atacttcgtt tcaatgaacg cattaaataa
    13381 taaataaaca cctcgcaaaa gaaagtagtc ctttcttatg gtgaggtgtt tatttgtcga
    13441 caacttgatt ataattgttt cattttttga ataagttcat aatcaggtgt agcatacaaa
    13501 gttttttgat tgtcatatgt tacaaaccca ggctttgcac ctgatggttt gtgcacattt
    13561 ttaattaatg tgtaatcaac aggtatttgt ccagaattac cagcttttga aaagtatcct
    13621 gctaacatag ccgcttcctt gattgtcgta tcacttggtg catcattaaa tataacgaca
    13681 tgtgaaccag gaatatcttt tgtgtgtaac catgtgtgag tttttttagc ttttttattt
    13741 gttaaataat cattttgctt gttattcttg ccaacatata tatcgtcgcc atcagttgat
    13801 acataatgtt gtaattgaat ctgcgctttc tttttcttag tttgattttt acgctgtttc
    13861 ataaagcctt gttctgctaa ttcatctcta atttcatcaa tgtcatggac agaaatatga
    13921 tgtaattgtt gttcgattgt tgaaaaataa tctatattat ctttcgtcaa ttgaatttga
    13981 tgttgtaatt cacgttctct cgttttcata cgattatatt gtttataata atattgagca
    14041 tttgctgatg gggattttgt aggatttaaa ggaatgacaa cttcttcatt cgtataataa
    14101 ttcaatgctg tcacttcttt atcgccttgc ttaattcgat atatattagc agtgatcaat
    14161 tcaccatata actgttcggt atctttattt ttagactgct catattcttc aatcaacttc
    14221 gctaatttat tttgatattt gtgcaactgc tgttgaacaa atcgaactaa atcattcgca
    14281 cgttgtttaa cgcgttcacg ttcgccacgc gcgtcataaa aacgatcaag taaatcattt
    14341 aatgaatcgt acgtaactgt atcatcatta aattgattta actttataaa atagaaatcc
    14401 tctttacctg tttcatgatt tttatgaaaa ataggagtag gtggtaactt ggtttctgcc
    14461 attacttcgt cgaatgcttc tggtaatgtt gatgaagtca taaattgacg acgactaacg
    14521 atttcattcg taattaaagg gctaaatcct tcaaactgat tcaataattg tttagcaata
    14581 ttacctgcgt taaaatcgat atatttcaac acctctgcac ctgtaatatc atacggattt
    14641 attttgtgct gagtaggtgg tgcttcataa ttaaatcctg gcattactgt acgatagtga
    14701 ttcgtatttg gtgttaagtg tttaaatcct tcaattattt tgcgattttc atctactaaa
    14761 attaagttac tatgtttacc cataatctca aggatgacag tgcggtaaat agtatcgcca
    14821 atttcatctt tactctttat atcgatttca atgcgacgat cattaccaat ttgcttaatc
    14881 gattcgataa taccaccttc taagtgtttt ctaaaaacac gcgcaaacat gggtggatta
    14941 aatggattat catatttttt agtagtcaat tgtaatcttg aaaagtttgg atggattgac
    15001 aataacaatt gatggttttg tctattttga cgtacaacca ttagtatcgt gtcattatca
    15061 ggttgattga ttttgtgaac gcgtcctgtt gttaaaaatt gtagagactc aaccattttc
    15121 tttgtaaata atccatcata agccataatt tatcttccac cttcttcata tcattcgttt
    15181 cattttaaca tgattgtaac ttttcggtat agttattgaa ccatacatta tgccacacat
    15241 tttacattat ctaaaagttc gcaattcaat ttatttaatt aaaattaatc gtgcaatata
    15301 acgatattgt tagacttaat agaaattatg gcatgcaatt tcaaatatgc tatacaatat
    15361 aaagaacaat gtgatatcat atttaaataa tagaagatta gcttagagag gtcgtaaggc
    15421 atggataatg aaaaaggatt gttaatcgtt ttatcaggac catctggagt aggtaaaggt
    15481 actgttagaa aacgaatatt tgaagatcca agtacatcat ataagtattc tatttcaatg
    15541 acaacacgtc aaatgcgtga aggtgaagtt gatggcgtag attacttttt taaaactagg
    15601 gatgcgtttg aagctttaat taaagatgac caatttatag aatatgctga atatgtaggc
    15661 aactattatg gtacaccagt tcaatatgtt aaagatacaa tggacgaagg tcatgatgta
    15721 tttttagaaa ttgaagtaga aggtgcaaag caagttagaa agaaatttcc agatgcgtta
    15781 tttattttct tagcacctcc aagtttagat cacttgagag agcgattagt aggtagagga
    15841 acagaatcca atgagaaaat acaaagtcgt attaacgaag cgcgtaaaga agttgaaatg
    15901 atgaatttat acgattacgt tgtagttaat gatgaagtag aacttgcgaa gaatagaatt
    15961 caatgtattg tagaagctga gcacttaaaa agagagcgcg tagaagctaa gtatagaaaa
    16021 atgattttgg aggctaaaaa ataatgttaa atccaccttt aaaccaatta acgtcacaaa
    16081 ttaaatcaaa gtatttaatt gcaacaactg cagcgaaaag agcgcgtgaa attgatgaac
    16141 aacctgaaac tgaattatta agtgaatatc attcatttaa accagttggt agagcgttag
    16201 aagaaattgc tgacggtaaa attagccctg ttatttcaag tgattattat ggtaaagaat
    16261 agtttcgacc attaaaaata tgtgaagcta gacgtacatc aaatacgtca aggcatatcc
    16321 aaatcaaacg cacctatgta ggtgtgtttt tttagtttat ccaatgattg atgttataat
    16381 aatactaaat ttgtatctat aaaaaagtaa tgagcatttg tgcgcatatg atgatgtaaa
    16441 gcgtaaatgt catagtgata tttaagggag attgaatatg aagaaaatat tattagccgt
    16501 tacaggtggc attgcggcat ataaagcaat tgatttgaca agtaagttaa cacaatctgg
    16561 gtatgaagtt cgcgttatgt taacgaatca cgcacaaaaa tttgtgacac cattagcatt
    16621 tcaagcaata agtcgaaatg ctgtttatac agatactttt atagaagaaa atccttcaga
    16681 aatacagcat attgcattag gtgattgggc agatgcaatc attgttgcac ctgcaacggc
    16741 aaatacaatt gcaaaattga gtgtaggtat tgctgatgat ttggtgacat caacgttgct
    16801 agcaacagag acaccgaaat ttattgcgcc tgctatgaat gtgcatatgt atgaaaataa
    16861 acgtacgcag caaaatatta atattttaaa agaagatggg tatcatttta tcgaaccagg
    16921 aagcggattt ctagcatgtg gttatgttgc taaaggacgt atggaagaac cgcttcaaat
    16981 cgtttctgtt attgatgctc attttcaaaa tagtaatcgt ttagctaata gttcatttca
    17041 agataaacgc gcattggtta cagcaggacc aactattgaa gttatcgatc cagtcagatt
    17101 tgtatccaat cgttcttctg gaaaaatggg ctatgcaata gctgaagcat tgcgaaatcg
    17161 aggagctatc gtgacgttag ttgctggtcc tacaacacta gaggatccaa aagatattga
    17221 agttattcat gttcaaagtg ctgaagaaat gtttgaacaa gtgacaagcc gatttgacga
    17281 acaagatatt gttgtaaaag cagcagccgt atctgactat acaccggttg atgtattaga
    17341 acataagatg aaaaagcagg atggtgattt gtcagtatct tttaaacgta ctaaagacat
    17401 tcttaaatat ttaggtgaac ataaaacatc acagtattta ataggctttg cagcagagac
    17461 tgaagatatt gaaaattatg cacaacaaaa attacgcaag aaaaatgcag atgtgattat
    17521 ttcaaataat gttggggata tgtctatcgg atttagttct gatgataatg aattgacaat
    17581 gcattttaaa aataatgaaa aggtaaatat caagaaagga aaaaaagtag tattagctgc
    17641 acaaatttta gatgaactag aaactaggtg gcaataatga tagcgaaagt catagtcgat
    17701 gtcgcgtcga agagcgttga ctataaattt gattatataa ttcccgaaca actcgaatct
    17761 gtcatccaac ctggtgtgcg tgtaattgta ccttttggac caagaacgat tcaaggttat
    17821 gtaatggaag taacagcaga acctgatgca caacttgacg tttcgaagtt aaaaaaaatc
    17881 atagaagtga aagatataca accagaatta acatcagaat taatagcttt aagtgagtgg
    17941 atgggttcaa ctcatgtcat taaacgtatt tctatgctag aagtgatgct tccaagtgct
    18001 attaaagcga agtataaaaa agcatttaag atgaaagatg acatagagct accttcagct
    18061 ttattacaaa aatttgataa gcatggttac tattattata aagatgcgca aaaaaataat
    18121 gatattcaat tgcttatgaa gttgttaaaa gatgatatcg ttgaagaaaa aacgattctc
    18181 acacaaaata taactaaaaa aaccaagcgt gctgttcgtg tcattgaagg gtatcatcct
    18241 gatgaagtat tagctaagtt ggagaaagtt attaaacaat acgatttgta tgcttacttg
    18301 tctgaagaac aacataaaac aatattttta actgatattg aggatatggg cttttcaaaa
    18361 tccagtttag atggacttat caaaaaaggt tatgttgaaa aatatgacgc ggttgttgaa
    18421 agagacccat ttaaagatcg tgttttcgaa caagaatcaa aacagcaatt aacagaagac
    18481 caatataaag catatgaagc gattaaagct aaaattgtaa gccaagagca agaaacattt
    18541 ttacttcatg gtgtgacggg atcaggtaaa acagaagtat atttacaaac gatagaagat
    18601 gttttaagcc aaggaaaaca ggcgatgatg ttagttcctg aaatcgctct aacaccgcaa
    18661 atggttttac gcttcaaacg tcgatttggt gatgacgttg ctgtattaca ttctggctta
    18721 tctaatgggg aacgttatga tgagtggcaa aaaattaggg atgatcgtgc gagagtaagt
    18781 gttggtgcaa ggtcaagtgt gttcgcacct ttcaaaaatt tagggttaat catcattgat
    18841 gaagaacatg aatctacata taaacaagaa gattatccga gatatcacgc tagagaaatt
    18901 gcccaatggc gaagtgaata tcatcactgt ccagtcattt taggaagtgc aacaccatgt
    18961 cttgaaagtt atgcacgagc tgaaaaagac gtttatcatt tgctatcatt accaaacaga
    19021 gtgaaccaac aagctttacc tgaaattgat atagtagaca tgcgtgaaga attgagtgaa
    19081 ggtaatcggt caatgttttc aaaagattta cgtgaagcca tacaattaag attagatcga
    19141 caggaacaag ttgttttatt tttaaatcga cgtggttatg catcgtttat gttatgtcgg
    19201 gattgtggat atgtaccgca atgtccaaac tgtgatattt cattaacgta tcataaaacg
    19261 acagacttat taaaatgtca ctattgtggt taccaagaga cgccaccgaa tcaatgtcca
    19321 aattgtgaga gtgaacacat tcgacaagta ggtactggta ctcagaaagt tgaagaacta
    19381 ttgcaacaag aatttgaaga tgcgcgcata attaggatgg atgtagatac aacctcaaag
    19441 aaaggtgcac atgaaaagtt attgactgaa ttcgaaaaag gtaacggtga cattttacta
    19501 ggtactcaga tgattgcgaa aggattagat tatccaaata ttactttagt tggtgtgctg
    19561 aatgcagata caatgttaaa tttacctgat tttcgggcga gcgaacgtac ttatcaacta
    19621 ttaacgcaag tggctggtag agctggtcgt catgaaaagg caggtcaagt catcattcaa
    19681 acgtataatc cagatcatta ttcaatattg gatgttcaaa aaaatgatta tttaacattt
    19741 tatcgtcagg aaatggaata tcgtaaatta ggaaagtatc caccgtatta ttatttgatt
    19801 aatttcacaa tctcacataa agaaatgaag aaggttatgg aagcatcgca gcatgttcat
    19861 aaaattttat tacagcattt aacagaaaaa gcgcttgtac taggtccatc tccggcagca
    19921 cttgcgagaa tcaacaatga atttagattc caaattttag tgaaatataa aagtgaacct
    19981 ggattattac aagccattca gtttttagat gactattacc atgaaaaatt tataaaagaa
    20041 aaattagcat tgaagattga tattgatcca cagatgatga tgtaacatta ctaattatta
    20101 gaaacaagtt cagtatgtac gagtatttga accagtgttt gaatatttac tttaagtaca
    20161 gaaaaagggc agaatataca actgttaact atttaaatta gcagtttata ttctgtcttt
    20221 ttatatggct ttataactta cgtgattttg gtttgataag gaatttatta gtattttcat
    20281 ttacgaattc tgattttaat
//
LOCUS       DAOBBH010000030        20123 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.030, whole genome shotgun sequence.
ACCESSION   DAOBBH010000030 DAOBBH010000000
VERSION     DAOBBH010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20123
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.030"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(126..680)
                     /locus_tag="P6L01_000796"
     CDS             complement(126..680)
                     /locus_tag="P6L01_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDP3029881.1"
                     /translation="MKRQAKIEIQNALVDLMAEYPFQEISTKMICAYCNINRSTFYDY
                     YKDKFDLLDTINSKHKEKFQFLLSALHHNFENIKQDKLKLYKFFIIIAKYIKHNEQFF
                     KDILVTYPMKTLFIDYINLARDYYQQIMNDYSTTVTNKQLYVTYIIGGQAGVFINWLS
                     NGCNESPEEVADILLANTIKLQIQ"
     gene            801..1448
                     /locus_tag="P6L01_000797"
     CDS             801..1448
                     /locus_tag="P6L01_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDP3029882.1"
                     /translation="MKKMILINVITVVVLLAIGIAGFYFWNKTTSYVTTDNAKVNGDQ
                     IKIASPASGQIKSLNVKQGDKLDKGDKVATVTVQGQDGETKDMDLKMPQKGTIAKLDG
                     MEGSMVQAGNPIAYAYNLDDLYVTANIDEKDIKDVEVGKDVDVTIDGQKASIKGKVDS
                     IGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLESEPSKQVVPGMNAEVKIHKN"
     gene            1461..3392
                     /locus_tag="P6L01_000798"
     CDS             1461..3392
                     /locus_tag="P6L01_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HDP3029883.1"
                     /translation="MTTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQ
                     SNQSKFKASDLDKTTDQSTQRMTHEELRVDNQDDHSQVSLNGYTKGSEKDQEAFTNNN
                     GEEAVAAKNPESEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLN
                     VALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIG
                     SLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILA
                     PAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILVGFVWFKLYQYTTNPKADIPGIIFS
                     TIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKSPMLNLEVLKFP
                     TFTLTTIINMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGK
                     LLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMVTA
                     AINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNPVV
                     QDHMRELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQSN
                     KKAKATAQKLEASSNNNHE"
     gene            complement(3638..4276)
                     /locus_tag="P6L01_000799"
     CDS             complement(3638..4276)
                     /locus_tag="P6L01_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029884.1"
                     /translation="MTNPNQRLEPFDETFQQPNIHRGKRYGKKKRSLVSMIIQIIVVI
                     LTAIAGYSMWKQPIFNIVFTKESVDFDGIKNFNETIGKMHQLNINLGNINDLQQGIDR
                     LILVFNGFFILCIVSLVITILTIVFNRTVLKVVNILVLTIMLVITLYFSYIIKTLAQE
                     IADALKKYYLNVPPDQVITEADAIHNGIILLGCSIGLLVISLFFRNRMPRLK"
     gene            4484..5404
                     /locus_tag="P6L01_000800"
     CDS             4484..5404
                     /locus_tag="P6L01_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDP3029885.1"
                     /translation="MIRNRVMNSVVNKYLLHNRSIMFKNDQDVERFFYKREIENRKKH
                     KQPSTLNVKANLEKLSLDDMQVFRFNFRHQIDKKILYIHGGFNALQPSPFHWRLLDKI
                     TLSTLYEVVLPIYPKTPEFHIDDTFQAIQCVYDQLVSEVGHQNVVVMGDGSGGALALS
                     FVQSLLDNQQPLPNKLYLISPILDATLSNKDISDALIEQDAVLSQFGVNEIMKKWANG
                     LPLTDKRISPINGTIEGLPPVYMFGGGREMTHPDMKLFEQMMLQHHQYIEFYDYPKMV
                     HDFPIYPIRQSHKAIKQIAKSIDEDVTQNN"
     gene            complement(5512..5931)
                     /locus_tag="P6L01_000801"
     CDS             complement(5512..5931)
                     /locus_tag="P6L01_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029886.1"
                     /translation="MTDALQQKIHIELLDLLDDVKFELTELNAQKGLYINGPANQLLK
                     RGVHMAYVQGQKQAIDNIMTIVEQQLEDQHFLEDYDKFQNEVTHRNYDKTANFAELSD
                     IPRQFDNFLDQFYQIKGQYFIITHINTLIGDFHSEAH"
     gene            complement(5928..6419)
                     /locus_tag="P6L01_000802"
     CDS             complement(5928..6419)
                     /locus_tag="P6L01_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDP3029887.1"
                     /translation="MVIIKQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYS
                     HGLSLKERENLAYTLFTLEEHTTYLSELSLGDVFTVTLYIYDYDYKRLHLFLTLTKED
                     GTLASTNEVMMMGINQHTRRSDAFPESFSTQIAHYYKNQPTITWPEQLGHKIAIPHKG
                     ALK"
     gene            6516..7463
                     /gene="corA"
                     /locus_tag="P6L01_000803"
     CDS             6516..7463
                     /gene="corA"
                     /locus_tag="P6L01_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDP3029888.1"
                     /translation="MALKIRYQTTYEPFKVVDDIKEIPKDATIVWYDFDEPNEQENEW
                     FKAHFNFNDLEVDDAINGMPRAKYKSYKDYQYLVFHSIMGSNYSPIALNIFIQDNVLV
                     TYHHQTLESLNKVVYKYMNTLDAELDCADVVILILDMMVDKYFNFVYALEDSVYHFED
                     RHVDDRFNKMVMDSVFKLRSDLIKVKRVLFPMQELIDTMKQNGDLIIDNKHSLYIQHI
                     DDHLIKQRNIIRTAQEMTNEIRENFESYTSFRMNSIMQVLTLVSVIFSPLTFIAGIYG
                     MNFVNMPALHLHYGYYICLAVMFVIAVVLIIFFRRKKWF"
     gene            7494..8543
                     /locus_tag="P6L01_000804"
     CDS             7494..8543
                     /locus_tag="P6L01_000804"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDP3029889.1"
                     /translation="MSDFQREQRKNEHVEIAMAQSDAMHSDFDKMRFVHHSIPSINVN
                     DIDLTSQTPDLTMAYPIYINAMTGGSEWTKNINEKLAVVARETGLAMAVGSTHAALRN
                     PRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPE
                     GNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNF
                     VDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALG
                     AKATGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPE
                     IMSWIEQRSLNIHRG"
     gene            complement(8591..9799)
                     /gene="gltS"
                     /locus_tag="P6L01_000805"
     CDS             complement(8591..9799)
                     /gene="gltS"
                     /locus_tag="P6L01_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDP3029890.1"
                     /translation="MIELNAITTLCLACILYLLGTAIVNHVNFLKRICIPAPVIGGLI
                     FAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKVLLLYFMFC
                     AIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
                     ALAAATLGLVFGGLIGGPVVKYLIKRYNLKPQHSDDTFKDYSQVAYNEHLHSKFNATE
                     VFFIQFTIVVFCMAVGSYFSHLFTAQTGINVPIYVGSLFVAVIVRNISESFNFNIVDL
                     KITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLG
                     KDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVI
                     VIMGFIQWFS"
     gene            complement(10132..10740)
                     /locus_tag="P6L01_000806"
     CDS             complement(10132..10740)
                     /locus_tag="P6L01_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDP3029891.1"
                     /translation="MDFVNNDTRQIAKNLLGVKVIYQDTTQTYTGYIVETEAYLGLND
                     RAAHGYGGKITPKVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEEG
                     LSAMFRNRGKKGYEVTNGPGKWTKAFNIPRAIDGATLNDCRLSIDTKNRKYPKDIIAS
                     PRIGIPNKGDWTHKSLRYTVKGNPFVSRMRKSDCMFPEDTWK"
     gene            11139..11759
                     /locus_tag="P6L01_000807"
     CDS             11139..11759
                     /locus_tag="P6L01_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDP3029892.1"
                     /translation="MEQLPQVGFMKSFVLFWKNYVNFKGRSRRSEYWYMALWHLILMV
                     PAVCVLIINLFLVFISIAADNETLIVMSILMIILTSVYTFVYSLATFIPNLALCVRRF
                     HDISRTMLFPMIMTVFSIIFYIVLQIIDSYYDSDFTLMPMGLNILLVLLYFVYLGLTI
                     VMIVFLCFDSKPANKYGESPKYPSTIKNQPVTSETPKTPEETDNQL"
     gene            11990..12889
                     /locus_tag="P6L01_000808"
     CDS             11990..12889
                     /locus_tag="P6L01_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3029893.1"
                     /translation="MGLVIEHVTKRFGKMTAVNDISLELESGKMLGFLGRNGAGKTTT
                     FRMILGLSEPTEGHITYNGKKLDKTMYNRIGYLPEERGLHGKLTVEEELKYLATLKGM
                     SKTEIQQQISYWLERFDITENRKKRIDSLSKGNQQKIQLLASMLHKPELLILDEPFSG
                     LDPVNVELLKEAVKDLNDWGSTIVYSSHRMEHVEELCDDVCILDKGQLVVSGDINHVR
                     ASNGNKKVVIESETTLPDLTNIRGIIHSENMKQGLQLTIENEDVAKDIYQVVAHQGYV
                     KRFQVVEPSLQDIFIEKVGGKDA"
     gene            12882..14114
                     /locus_tag="P6L01_000809"
     CDS             12882..14114
                     /locus_tag="P6L01_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3029894.1"
                     /translation="MHKFWATFTLTYIKKIKAKSFVIFMIIIAALMIGLSNIDKIINM
                     FDDGPDKIGVAAPNEQIYKVFKQQANTFHSDAKFTKVSIEDAEKEVKKHKLDKAYIIK
                     VNQNRTLQGTIISEKRVSHEDSQKVQALLTAIQTNMVAGELNINKEDLQKLQAQSKVD
                     NKVISNDEVDKVSEGQKIFNYALAYGIIFLMFFIVLNYASQIAMEIASEKTSRVIEMI
                     ITSISPIQHIFAKILGVIAVAVTQIILIVLMAVICIFAFDLKDLLQGFNVEMNQLSWQ
                     IIIVGIISSIVGIMAYVLLAAILGSLTSRIEDLNQSLMPLTLLGMIAFYIAIFTINNP
                     DGQLALITSYIPFLAPFQLVVRAQTSGLQIYEVILSSLISIVMVAVLIWIAIKTYKDS
                     VLTFERGLFNSMKRVFKK"
     gene            14098..14283
                     /locus_tag="P6L01_000810"
     CDS             14098..14283
                     /locus_tag="P6L01_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029895.1"
                     /translation="MYLKNKIDESDTHLSYKMMMKACYSYFFIFSYRIMSKKVYNMNV
                     KTNFLKWECEVRRNVNK"
     gene            14332..14658
                     /locus_tag="P6L01_000811"
     CDS             14332..14658
                     /locus_tag="P6L01_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HDP3029896.1"
                     /translation="MLYPIFIFILAGLCEIGGGYLIWLWLREGQSSLVGLIGGAILML
                     YGVIATFQSFPSFGRVYAAYGGVFIIMSLIFAMVVDKQMPDKYDVIGAIICIVGVLVM
                     LLPSRA"
     gene            14688..15707
                     /locus_tag="P6L01_000812"
     CDS             14688..15707
                     /locus_tag="P6L01_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDP3029897.1"
                     /translation="MIVEVEHQRQGVDLIKIDNDETKIIFTNYGARVVSWKYHDNNIV
                     LGNVVEADEFYHSNPFKFGASIGRYSGRIDNAKFKLKGKEYQLEKNNGEHHLHGGCHG
                     LDNKLFDYEIRNEIAQIKVIFKTVLKSADDHFPGDIDVTITHIYDADHQWSIEYEAVA
                     SEDTLFSPTNHVYFNLNRDNNVVDNHRISSNQLDMYVLDERNIVTGDILDLHEVFEDN
                     KIKLSDIFTSQHAQLSQQMTRFGGLDHPFTVGEHKMYVENHEFMLEVDTDMPHVVFFT
                     FNQPDEWDSPFNIYKPHSGFTLETQFLPNDINLYGEKAPSILKAGEVFNSKTSFRIEE
                     KVEND"
     gene            complement(15943..16596)
                     /locus_tag="P6L01_000813"
     CDS             complement(15943..16596)
                     /locus_tag="P6L01_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDP3029898.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPIRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPTLPTHAMFGENLTFDYLDESDVYFGN
                     QYRLGEALIEVSEIREPCWKIQAKYNIPDLVKRMSTSGKTGFYFRVLKQGYVSPNDQL
                     YLIQEAPIEHRLSVQQLNDLYYNDRQNQDMLRYALNNPFLSPTRRDKLQKMYNRTLK"
     gene            16770..17456
                     /locus_tag="P6L01_000814"
     CDS             16770..17456
                     /locus_tag="P6L01_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HDP3029899.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKIVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            complement(17522..18781)
                     /gene="sdpC"
                     /locus_tag="P6L01_000815"
     CDS             complement(17522..18781)
                     /gene="sdpC"
                     /locus_tag="P6L01_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HDP3029900.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSIIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKVQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYGTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDAEKTGVATASTVGVAKNDTENTVADEPSIHEGTEKTEP
                     QHHIGNQTESNHDEDHDITSESVESAESVKQAPQSDDLTNDSNEDEIEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            19071..20006
                     /locus_tag="P6L01_000816"
     CDS             19071..20006
                     /locus_tag="P6L01_000816"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HDP3029901.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSMPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTDYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSH
                     VSPTIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKFVANLVHHFLK"
BASE COUNT         6989 a         2851 c         3626 g         6657 t
ORIGIN      
        1 attattatgt ggaaatctcg gcatttttat attgatagca ccgtaacata tgtactatta
       61 tctatccatt tggtttaact ttatctagtt gttttatagc atttctttat tagaatgaaa
      121 ccgtattatt ggatttgaag tttaatcgta ttagccaata aaatgtcagc aacttcttca
      181 ggtgattcat tacaaccatt cgaaagccaa ttaataaaca ccccagcttg accgcctatg
      241 atatatgtaa cataaagctg tttattggtt actgtagtac tatagtcatt cataatttgt
      301 tgatagtaat ctctcgctaa atttatatag tctatgaata atgttttcat tggataggta
      361 actaaaatgt ctttgaaaaa ctgctcattg tgcttgatat acttagcaat aataatgaag
      421 aatttataaa gtttaagctt atcctgttta atattttcga aattatgatg tagcgcgctt
      481 agaagaaatt ggaatttttc tttatgcttt gagttgatgg tatccaataa atcaaattta
      541 tctttgtaat aatcataaaa agtagaacga ttaatattac aatacgcgca aatcatttta
      601 gtggatattt cttgaaaagg atattcagcc atcaaatcaa caagtgcgtt ttgaatttct
      661 attttggctt gtcgtttcat attgttacac ctttgtgaat tttaaccaac actcttataa
      721 aaattgttgg tgttttttta gttatcatgt ctctataata aataaagtga attttaaaag
      781 caaataggag gaaatgtttc atgaaaaaga tgatattaat taatgtcatt acggttgttg
      841 tactgttagc aattgggatt gctggctttt atttctggaa taaaacaaca agctatgtaa
      901 caactgataa tgcaaaagta aatggcgatc aaattaaaat agcaagtcct gcatctggac
      961 aaattaaatc acttaatgtt aaacaaggtg acaaactcga taaaggtgac aaagtagcaa
     1021 ctgttactgt acaaggtcaa gatggcgaaa cgaaagatat ggatcttaaa atgcctcaaa
     1081 aaggtacaat tgccaaatta gatggtatgg aaggttcaat ggtgcaagct ggtaacccaa
     1141 tcgcttatgc atacaattta gatgatttat atgtaacagc aaatattgac gaaaaagata
     1201 ttaaagatgt tgaagtaggt aaagatgttg atgtgactat tgacggtcaa aaagcttcta
     1261 ttaaaggtaa agttgatagt attggaaaag caactgctgc aagcttttca ttaatgccat
     1321 catcaaatag tgatggtaac tacactaaag tttctcaagt aatacctgtt aaaatcactc
     1381 tagaatcaga accgtctaaa caagttgttc ctggaatgaa cgctgaagta aaaatccata
     1441 aaaattaagg gggtcattaa atgactacga ccttcattat tagctacatt attttagcgc
     1501 tcattattgt tggggttatc aatttatttt taataagatc aagaaaaaaa ggcaaacgcc
     1561 aacaaaagga acaacaattt acgacacgtc aatcaaatca atctaaattt aaagctagtg
     1621 atttagacaa aacaactgat caatcgacac aacgtatgac gcatgaagag ttgcgtgttg
     1681 acaatcaaga tgatcatagc caagttagtc taaatggtta cacaaagggg tctgagaaag
     1741 atcaagaagc attcactaat aataatggtg aggaagcagt tgctgctaaa aatcctgaat
     1801 cagaagaata taaagtgaat gaaaaaataa aaaaagaaca taaaaacttt atttttggtg
     1861 aaggtgtttc acgcggtaaa atattagcgg cattattatt cggtatgttt attgcgattt
     1921 taaaccaaac attattaaat gttgctttgc caaaaattaa tacggaattt aatatttctg
     1981 cgtcaacagg gcaatggttg atgacgggat tcatgttagt aaacggtatt ttaataccaa
     2041 ttacggcgta tctatttaat aaatattcat atcgaaaatt atttttagtt gcgttagtac
     2101 tctttacaat tggttcatta atctgtgcga tttcaatgaa tttcccaatc atgatggttg
     2161 gacgtgtatt acaagccatt ggtgcaggtg tactaatgcc attaggttca attgttatta
     2221 ttacaattta tccacctgaa aaacgtggtg ctgccatggg tacaatgggt atcgcaatga
     2281 ttttagcacc tgcaattggt ccaacattat caggttatat tgtacaaaat taccattgga
     2341 atgtgatgtt ttacggtatg ttcattatcg gtattattgc aatcttagtt ggttttgtat
     2401 ggtttaagtt atatcaatat acaacaaacc ctaaagctga tatcccaggt atcattttta
     2461 gtacgattgg ttttggtgct ttgttatacg gcttttcaga agcggggaac aaaggatggg
     2521 gttctgttga gatagaaaca atgtttgcga ttggtattat ctttattatt ctatttgtaa
     2581 taagagaatt aagaatgaaa tcaccaatgt tgaatttaga agtattaaaa ttcccaacat
     2641 ttacattaac aacaattatt aatatggttg taatgttaag tttatatggt ggtatgattt
     2701 tattaccgat ttatttacaa aatttacgcg gattctcagc attagattcc ggattgttat
     2761 tattacctgg ttctctaatt atgggtctac ttggaccatt tgcaggtaaa ttgttagata
     2821 ctattggttt aaaaccatta gctatctttg gtattgctgt catgacttat gcaacatggg
     2881 aattaactaa attaaatatg gatacaccat atatgacaat catgggtatc tatgtacttc
     2941 gttcatttgg tatggcattt ataatgatgc caatggtaac tgcagctatt aatgcgttac
     3001 cgggacgact tgcctctcat ggtaatgctt tcttaaatac gatgcgtcaa ttagcaggct
     3061 ctataggtac agcaatctta gttactgtta tgacaacaca aacaacacaa catttatcag
     3121 cttttgggga agagttagat aaaacgaatc ctgttgtaca agatcatatg cgtgaattag
     3181 catcgcaata tggtggtcaa gaaggcgcaa tgaaagtgtt attacaattt gtaaataaac
     3241 tagcaacagt tgaaggtatt aatgatgcat ttatagttgc aacgatattt agcatcatcg
     3301 ctttaatttt atgtttattt ttacaaagta ataaaaaagc aaaagctaca gctcaaaagt
     3361 tagaagcaag ctcaaataac aatcatgaat aaataaaata aattaattga agtgtgacta
     3421 atcaaaaatt atgttgtggg gacatgattt ttaaagtatc ggtgccaaat atggttatcg
     3481 atactttttt tatttgttga tttataaaat aaaagaggaa ttatattaaa atttggcatt
     3541 gacgtagtag gtcattaata aagaaaaagc aggaagtggg tcaacgaaat gaattttgtg
     3601 aaaataacat ttctgtccca atccctacta tataacatta ttttaaacga ggcatgcgat
     3661 tacggaagaa taagcttata acaagtaaac cgatgctaca gcccagtaaa atgatgccgt
     3721 tatgaatagc gtcagcttct gtaatcactt gatctggtgg tacatttaaa taatattttt
     3781 ttaaagcatc tgcaatttct tgtgcgagtg ttttaataat ataactgaaa tacaaagtaa
     3841 tgactaacat aattgttaac actaaaatat ttactacttt tagaactgtg cgattaaata
     3901 caattgtcag tattgttatg actaggctaa cgatacatag aataaagaaa ccgttaaaga
     3961 ctaaaattaa gcgatcgatt ccttgttgta aatcattgat attacccaaa ttaatattta
     4021 attgatgcat ttttccaatt gtctcgttaa aatttttaat gccatcaaaa tctacagatt
     4081 ctttggtgaa gacaatatta aaaatgggtt gtttccacat gctgtatcca gcgattgcgg
     4141 ttaatataac aacaatgatt tgaataatca tgcttaccaa tgaacgtttt ttcttaccat
     4201 atcgcttacc acgatgaata ttcggttgtt gaaatgtctc atcaaatggt tctaatcgtt
     4261 gatttggatt tgtcatgcaa ggcacctccg tataaagttc ttttctttac ttatcaatat
     4321 gtctagttgt ctgttataat gatgaaaaat cactgttcaa ttgtagtctt aaatttgaaa
     4381 gtgaaaaaga aaatatcttc tacattatat actttttttg gcaaagaatg taggggatag
     4441 acaatcattg gatacataaa atatataaag gtaggttaag acattgatta gaaaccgtgt
     4501 tatgaatagc gttgtcaata aatatttgct tcacaatcgc tcgattatgt ttaaaaatga
     4561 tcaagatgtt gaaagatttt tttataaaag agaaattgaa aatagaaaaa aacataagca
     4621 gccttcaaca ttaaatgtta aagcaaattt agaaaaatta tcattagatg atatgcaagt
     4681 ctttcgcttt aatttcagac atcaaattga taagaaaatt ttatatattc acggtggatt
     4741 caatgcacta caaccatcac cgttccattg gagattgttg gataaaatca ctttaagtac
     4801 attatatgag gttgtactgc ctatctatcc aaagacacca gagtttcata ttgacgatac
     4861 tttccaagcg atacaatgtg tttatgatca attagtatct gaagtaggac atcaaaatgt
     4921 cgtagtcatg ggtgatggtt caggtggtgc actggcatta tcctttgtac aatctctttt
     4981 agataatcaa cagccattac cgaataaatt gtatttaatc tcaccaattt tagatgcaac
     5041 actatctaat aaagatattt cggacgcctt aatcgaacaa gatgcggttc taagtcagtt
     5101 tggtgtcaat gagattatga aaaaatgggc gaatggccta ccattaacag ataagcgcat
     5161 atcgccaatt aatggcacaa tagaaggatt gccaccagtt tatatgtttg gtggtggacg
     5221 tgaaatgaca catcctgata tgaagctatt cgaacaaatg atgttgcaac atcatcaata
     5281 cattgagttt tatgattatc ctaagatggt acatgatttt ccaatttatc caattagaca
     5341 atcacataaa gctattaaac aaattgccaa atcgatagat gaggatgtaa cacaaaataa
     5401 ctaataacat acaattgacc cagttacgat ttcatgcaag atgatatttg acatgaaaac
     5461 gtaattgggt tttgtttttg aaaatatagc aagggttata tagactaatt gttaatgagc
     5521 ttctgagtga aaatcaccaa taagtgtatt gatatgtgtg atgataaagt attgcccttt
     5581 aatttgataa aattgatcta aaaaattatc gaattgacgt ggaatatcac taagttctgc
     5641 aaagttagct gttttatcat aattgcgatg agtaacctca ttttgaaatt tatcataatc
     5701 ttctaggaaa tgttgatctt caagctgctg ttccacaata gtcataatat tatcgatggc
     5761 ttgcttttgt ccttgaacat aagccatatg aacgccacgc ttaagtagct gatttgctgg
     5821 tccgttaatg tataaccctt tttgtgcatt taattctgtt aattcaaact taacatcatc
     5881 taataaatct agtaattcaa tatgaatctt ttgttgtaat gcatctgtca ttttaatgct
     5941 cctttgtgtg gaattgctat tttatgtcct aattgttcag gccaagtgat agttggttga
     6001 tttttataat agtgtgctat ttgtgttgag aatgattcag gaaaagcatc agaacgacgt
     6061 gtgtgctgat taattcccat catcattact tcatttgttg atgctagtgt accatcttct
     6121 ttagttaatg ttaaaaataa atgcaaccgc ttataatcgt aatcataaat atataaagta
     6181 acagtaaata catcgccaag agacaattct gagaggtatg tcgtatgttc ttctagtgta
     6241 aatagtgtat atgctaaatt ttcgcgttct tttaaagaaa gaccgtggct gtaattaaaa
     6301 cgattcacga cgtcactaaa aatgatatta taatttgcat catgcatatg attgttatgg
     6361 tcaatgaatt cagatgttac ggtttgagta tgtgtaaaaa gttgtttgat aataaccatc
     6421 tccttagtga ctatcatact aaaataaatt aagacataca cgttatttaa ttcatgataa
     6481 aataatagag aattcgttat ttagggggag tatcgatggc tttaaagata cggtatcaaa
     6541 ctacatatga accgtttaaa gtagttgatg atatcaaaga gataccaaaa gatgcgacta
     6601 ttgtttggta tgattttgat gaaccgaatg aacaagaaaa tgaatggttt aaagcacatt
     6661 ttaattttaa tgatttagaa gtggatgatg caattaacgg tatgccacga gctaaatata
     6721 aatcttataa agattatcaa tacttagtat ttcatagtat tatggggtca aactattcac
     6781 caatagcact aaatattttt atacaagaca atgtattagt aacatatcat catcaaacgt
     6841 tggaatcatt aaataaagta gtctataagt atatgaatac attagatgca gaattagatt
     6901 gtgcagatgt cgtcatatta attttagata tgatggttga taaatatttc aactttgtat
     6961 atgcacttga agatagtgtt tatcattttg aagaccgcca tgtagatgat cgctttaata
     7021 aaatggtcat ggatagtgtc tttaagttac gttcggattt gattaaagta aaacgtgtac
     7081 tgtttcctat gcaagagcta attgatacaa tgaaacaaaa tggtgatttg attatagata
     7141 ataaacattc gctatacatt caacatatcg atgaccactt gattaagcaa cgtaatatca
     7201 ttcgtactgc tcaagaaatg acaaacgaaa tacgagaaaa ttttgagtca tacacgtcat
     7261 ttagaatgaa tagtattatg caagtattaa cgttagtatc agttatattt tcaccgttaa
     7321 cattcatagc tgggatttat ggtatgaact ttgtaaatat gccggcatta catttacatt
     7381 atggatatta tatttgctta gctgtaatgt ttgttatagc tgtcgtatta atcatctttt
     7441 tcagacgtaa aaaatggttt taaattagaa atgacgaaaa ggtaggttta atcatgagtg
     7501 attttcaaag agaacagaga aaaaatgaac atgttgaaat agcaatggcc caatctgacg
     7561 caatgcattc agattttgat aagatgcgtt ttgtgcatca ttctatccca tcaattaatg
     7621 taaatgatat cgatttgaca tcacagacgc ctgacttaac gatggcatat ccgatttata
     7681 ttaatgcaat gacgggtggt agcgagtgga cgaaaaacat caatgaaaag ctagctgtag
     7741 ttgcaagaga aactggctta gcgatggcag ttggatcaac acatgcggca ttgagaaatc
     7801 cacgcatggc tgagacgttt acgattgcgc gaaaaatgaa tcctgaaggc atgattttta
     7861 gcaatgttgg tgcggacgta ccagtagaaa aggctttgga agcagttgaa ttacttgagg
     7921 cacaagcgtt acaaatccat gttaattctc ctcaagaatt agttatgcct gaagggaatc
     7981 gtgaatttgt gacttggtta gataatatag cgtcgattgt atcacgagtg tctgttccag
     8041 tcattataaa agaagttgga tttggtatga gcaaagaatt aatgcatgac ttacaacaaa
     8101 taggcgtcaa gtatgtcgat gttagtggca aaggtggtac taactttgta gatattgaaa
     8161 atgaacgtcg tgcaaataaa gatatggatt acttatcatc atggggacag tctacagttg
     8221 agtcattact tgaaacaacg gcttatcaaa gcgaaatttc agttttcgcg agtggtggtt
     8281 tacgtacacc actcgatgca attaaaagtt tagcacttgg tgcaaaggca actggtatgt
     8341 cacgtccgtt tttaaatcaa gttgaaaata atggcattgc acatacagta gcttatgtag
     8401 aatcatttat tgaacacatg aaatcaataa tgacgatgtt agatgctaaa aatattgacg
     8461 acttaacaca aaaacaaatc gtatttagtc cggaaataat gtcatggata gaacaacgta
     8521 gcttgaatat acatcgagga taaataaaag aggaggcggt ggcgtcgtct tttatatatg
     8581 agtttggtgt ttaactaaac cattgaatga atcccataat gactataaca ccaattaaat
     8641 cgattaagaa tgcaccaaca ataggtacaa ctaaatatgc tttaggtgag tttccatatt
     8701 ttttagtaat aacatctaaa tttgccatgg catttggcgt tgcaccaagc ccatgcccga
     8761 taaaaccacc taccattact gcagcatcat aatcttttcc taaacctcta aataaaatta
     8821 aaacagcaaa taaaatcata acgacaactt gaactaaaac gataataata agaggtatag
     8881 caagtttata aatttcgatt aattgaatgc tcattagcgc aagagataag aaaataccta
     8941 atgcgacatc gccaatttga ttagtaattt ttaaatctac aatattaaaa ttaaaacttt
     9001 cagagatatt tcggacgata acagctacaa ataatgagcc aacgtaaatt ggaacattaa
     9061 tccctgtttg agctgtaaac aaatgactga aataacttcc aactgccata cagaatacaa
     9121 cgattgtaaa ttgaatgaag aatacttcag tggcattaaa tttactatgt aaatgttcgt
     9181 tatatgctac ttggctataa tctttaaatg tatcgtcact atgttgaggc tttaagttat
     9241 aacgtttgat taaatatttt acaactggac caccgataag ccctccaaat acaagaccta
     9301 aagttgcagc tgcaagagcc gctgtcagtg ccgaatcaat acctaaatct tgaattgtct
     9361 taccataagc agcagcatta ccatgaccgc cttccataga catggaacct gctgttaatc
     9421 ctagtaacgg tttaatattt aatacttttg ctagtgatac accaactatg ttttgaatga
     9481 ctgaaatgat agcacaaaac ataaagtata atagcaagac tttgccacct aatttaaata
     9541 atttcaatga tgcaccaaga ccgattgtcg taaagaatgc taacatgaag aaatcttgaa
     9601 tgaatgaagc atctaattta atcttaacca tgccaaatga atccaaagcc gcaactaaaa
     9661 tagcaaagat taagccgcca atcactggtg ctggtataca aatgcgtttc aaaaaattaa
     9721 cgtgattaac gatagctgta ccaagtaagt aaagtataca ggctaaacat aatgttgtaa
     9781 ttgcattaag ttctatcatt ttcaaatccc ccttgttgaa tatccctctc cctaaataag
     9841 cttttatatg taagcgttat aaaagggcac ttttaacatt atacatgcaa caaatcaata
     9901 tttctagttt ttgaaaataa tatcaataat attaatgcat ctaaatttaa taattataaa
     9961 taattctatt gttatatttt ataattaaga acaagttcaa aaagaggaga tgaatcaaaa
    10021 aatatgatgg acaaaaaaac aatcaaccca tccgcatgtt ggcggcacga ttgattgact
    10081 tataaggaga tagatttcat tttcatgata gtaatcaatg aaagatggca tttatttcca
    10141 agtatcttcg ggaaacatac aatctgattt acgcatgcga gacacaaatg gattaccttt
    10201 cactgtgtaa cgtaaagatt tatgtgtcca atcaccttta tttggaatac cgattcgtgg
    10261 actagcaata atatctttag gatatttacg attcttagta tcaatagaca atctacagtc
    10321 atttaacgta gcgccatcga tagcccgtgg aatgttaaat gccttagtcc attttcctgg
    10381 gccattcgtt acctcgtagc ctttcttacc tctgttacgg aacatagcgg ataaaccttc
    10441 ttctggttca attgcgcgta taagtacgcc ttcaggtata ccttcagatt ttgttacaaa
    10501 attaatgagt aaatgcgtat gcatgacatg tgcataaatt gtaccaccac gtttatataa
    10561 tgacgtgact ttaggtgtta ttttaccgcc atagccatga gccgcacgat cattcaaacc
    10621 taagtaagct tccgtttcca cgatgtagcc tgtatacgtt tgagtggtat cctgataaat
    10681 cactttgaca cctaataagt ttttagcaat ttgtcttgta tcattattaa cgaaatccat
    10741 aatattctcc tcgcaattat atgattaaat taaataaaaa tgaaaaagat atttttattg
    10801 tggtaaatat ttgataaaat agtattgtta agaattataa aaatgtaaag tatatacttt
    10861 gttatggaat attttatttg cagagattga gactctttta aacgtgaaca ttgcagtatg
    10921 acatattttt agtatagtta acgaagcgtt gactttcaaa tgagtctcca tatagatgtg
    10981 tacttgtagc gggttttcat tttactagtg atgaaatata aagtccattt tccaaagtga
    11041 atgacgattg aaatgaagtg gcttgataat gaatcttgta aattataacg gtaacaataa
    11101 ttaaaaatat aaatatgatt aacgaaaggg agattacaat ggaacaatta cctcaagtag
    11161 gttttatgaa atcattcgta ttattttgga aaaattatgt aaattttaag gggagatcaa
    11221 gacgtagtga atattggtat atggcactat ggcatttgat acttatggtt ccagctgtgt
    11281 gtgtgctaat aataaaccta tttctagtct ttatttctat agcggcagat aacgaaacat
    11341 taatagttat gagtatattg atgattatcc ttacatctgt gtatactttt gtctatagtt
    11401 tggcaacgtt tattccaaat ttagcgttgt gcgttcgaag atttcacgat atatcaagaa
    11461 caatgttatt tccaatgatt atgacagtat tcagtattat attttatata gtattacaga
    11521 ttatcgatag ttattatgac agtgatttta cattaatgcc gatgggtctt aatattttgt
    11581 tagtacttct ttattttgtt tatttaggcc taacaattgt tatgatagta ttcctatgct
    11641 ttgacagtaa gccggcaaat aaatatggcg aaagcccaaa atacccgtct acaattaaaa
    11701 atcaaccagt gacatctgaa acaccgaaaa caccggaaga aacagataat caattgtaat
    11761 gatataaata tctcggcaca atgatgctgg gatatttttt tacaaataag gcaataaagg
    11821 tatgggaaat gcgtatctag cgctaatgat acttaacatg caaagaagat actatttaaa
    11881 tatttcaatt tcagaaattt taaaaataaa ttcactttaa aaatattgca tttatagaga
    11941 atagaaatgt tatattaaga taaatacata tttttttagg aggcaaagta tgggacttgt
    12001 aattgaacat gttacgaaac gttttggcaa gatgacggca gtaaatgata tttcattaga
    12061 gttagaatca ggcaaaatgt taggttttct aggaagaaat ggtgcaggta aaacaacgac
    12121 atttcgtatg attttaggct taagtgaacc aactgaagga cacattacgt ataacggtaa
    12181 aaaactagat aaaacaatgt acaatcgtat tggttattta ccggaagaac gcggtttaca
    12241 tgggaaattg acagttgaag aagaattgaa atatttggca actttaaagg gaatgtcaaa
    12301 aacagaaata caacagcaaa tatcgtattg gcttgagcgt tttgatatta ctgaaaaccg
    12361 caaaaaacga attgatagtc tatcaaaagg gaatcaacaa aaaattcagc tgttagcaag
    12421 tatgttacat aaaccagaat tactaatatt agatgagcct ttcagtggtc tagacccagt
    12481 taatgttgag ttattaaaag aagcagttaa agacttaaat gattggggca gtacaatcgt
    12541 ttatagttct caccgtatgg agcatgttga agaattatgt gatgatgttt gtattttaga
    12601 taaaggtcaa cttgttgttt ctggtgatat caatcatgta cgtgcatcta atggtaacaa
    12661 gaaagttgtc attgaatcag aaacaacact accagattta acaaacatta gaggcatcat
    12721 tcatagtgaa aacatgaagc aaggtcttca gttgacgatt gagaatgaag acgtagccaa
    12781 agatatttat caagttgtgg cacatcaagg ttatgttaaa cgattccaag ttgtagaacc
    12841 atcattacaa gatatattca ttgagaaggt tggtggcaag gatgcataag ttttgggcga
    12901 catttacttt gacatatatc aaaaaaatta aagctaaatc atttgtaatt tttatgatta
    12961 ttattgctgc cttaatgatt ggcctatcta acattgataa gattattaac atgtttgatg
    13021 atggtccaga taaaattggt gtagcagcac cgaatgaaca aatctataag gttttcaagc
    13081 aacaggcgaa tacattccat agtgatgcta agtttactaa agtttctata gaagatgctg
    13141 aaaaagaagt aaaaaaacat aaacttgata aagcttatat tatcaaggtg aatcaaaatc
    13201 gcacgttaca agggacaatc attagtgaga agcgagtaag tcatgaagat tcacaaaaag
    13261 tacaagcact gcttacagct attcaaacaa atatggtagc cggggaatta aatatcaaca
    13321 aagaagactt acaaaaatta caagcacaaa gtaaagttga taataaggtg atttctaatg
    13381 atgaagtcga taaagtatca gaaggacaaa agattttcaa ctatgcttta gcttatggca
    13441 ttatctttct aatgttcttc attgttttaa attatgcatc tcaaatagct atggaaattg
    13501 ctagtgagaa aacatcgcgt gtcattgaaa tgattattac aagtatttca ccaatccaac
    13561 atatttttgc taaaatttta ggcgttatag cagttgccgt gactcagatt attttaattg
    13621 tattgatggc agttatatgc atttttgcct ttgacttaaa agacttgcta caaggtttta
    13681 acgtggaaat gaatcaattg tcttggcaaa ttattattgt cggaattata agtagtattg
    13741 ttggaattat ggcgtatgta ttacttgcag ccatcctcgg atcattaaca tctagaatag
    13801 aagatttaaa tcaatctttg atgccactga cattacttgg tatgattgca ttttatatcg
    13861 cgatattcac gataaataat cctgatggac aacttgcttt aatcacaagt tatattccgt
    13921 ttttagcgcc atttcaatta gttgtacgcg ctcaaacttc agggcttcaa atatatgagg
    13981 tcatcttaag ttctttaatc tcaattgtaa tggtagctgt cttgatatgg attgcaatta
    14041 aaacatataa agattcagta cttacatttg aacgtggctt atttaattca atgaaacgtg
    14101 tatttaaaaa ataaaattga tgagagtgac acacatttat catataaaat gatgatgaaa
    14161 gcgtgttact cttatttttt tatatttagc tatagaataa tgtctaaaaa agtgtacaat
    14221 atgaatgtga aaacgaactt tttaaaatgg gaatgtgaag taagaagaaa tgtaaacaaa
    14281 tgatatgtag ccgatagatt attgatagca aatgtgtgat ggagtgattt catgttatat
    14341 ccgattttta tatttatatt agctggctta tgtgaaatag gtgggggata cctgatttgg
    14401 ctgtggctta gggaaggaca gtcttcactt gttgggctaa taggcggtgc gatactcatg
    14461 ttatatggtg tcattgcgac atttcaatca tttccatcat tcggaagagt atatgcagca
    14521 tatggtggcg tatttatcat catgagtttg atttttgcga tggtcgtaga taaacaaatg
    14581 ccagataaat atgatgttat cggtgcaatc atttgtattg taggtgtttt agtaatgttg
    14641 ctacccagca gagcttgaac acttgtggtt agaagggaga agtaaaaatg atagttgaag
    14701 ttgagcacca aagacaaggt gtagatttaa tcaaaattga taatgatgaa actaaaatta
    14761 tttttacgaa ttatggagca agagtagtgt cttggaaata tcatgataat aatattgttc
    14821 ttggaaatgt tgttgaagcg gatgagtttt atcatagtaa tccttttaaa tttggtgctt
    14881 ctataggaag atatagcggt agaattgata atgctaaatt taagttgaaa ggtaaagaat
    14941 atcagttaga aaaaaacaac ggtgaacatc atttacatgg tggatgtcat ggtttggaca
    15001 ataagttatt tgattatgaa attagaaatg aaattgctca aattaaagtt atatttaaga
    15061 cggtgttgaa atcagctgat gatcatttcc cgggtgatat cgatgtgacg attacacata
    15121 tttatgatgc ggatcaccaa tggtcgattg aatacgaagc agttgcatca gaagacacat
    15181 tgtttagtcc tacgaatcat gtgtacttca atttgaatcg agataataat gtcgttgata
    15241 atcatcgcat ttcaagtaat caattagata tgtatgtatt agatgagcga aatattgtta
    15301 caggtgatat tctagattta catgaagtgt ttgaagataa caagataaaa ttgagtgata
    15361 tttttacatc acaacatgcg caattaagcc aacaaatgac acgttttggt ggtctcgatc
    15421 acccatttac agttggtgaa cataaaatgt atgttgaaaa tcatgaattt atgttagagg
    15481 tcgatacaga tatgcctcat gttgtgttct ttacttttaa ccagcctgat gagtgggata
    15541 gtccatttaa tatttataaa ccacattctg gctttacatt agaaacacaa tttttaccaa
    15601 atgatattaa tttatatggt gaaaaagcac cgtcaatttt aaaagctggt gaagtgttca
    15661 attcgaaaac aagtttccgt atcgaagaaa aagtagaaaa tgattaaaag tatcggcatt
    15721 tatataatat ttatgttatt tggcggaatt ttgaataggt tatcaagaat cgtatcaaat
    15781 gaagtgacat cattaatata cagtttgaaa taaagaaaat atataacata aaaaaggaat
    15841 caatagcatc agtggttata gataagacta ttatacaaac gatgtgtaag ttgattcctt
    15901 tttgttttga aaagttcatt taacacttat aatgaaaggt aattatttca atgttctgtt
    15961 atacattttt tgaagtttat cgcgtcttgt tggtgacaga aatggattgt ttagtgcata
    16021 tcgtaacata tcttgatttt gtctatcatt ataataaagg tcattaagct gttgtacaga
    16081 taaacgatgt tcgattggtg cttcttgtat taagtaaagc tgatcatttg gagatacata
    16141 gccttgtttt aatacccgga aatagaaacc tgttttacca gatgtagaca tgcgcttcac
    16201 taaatcagga atattatatt ttgcttgaat tttccagcat ggttctctaa tttcagaaac
    16261 ctcaattaac gcttcaccta aacgatattg attaccaaag taaacatcag attcgtctaa
    16321 ataatcaaat gttaaattct ctccaaacat cgcatgagta ggtagtgtag gtaaatcatc
    16381 ttgatacagt gcataattat gcttactaaa cccacaaact gctttatgtg gtccaccatg
    16441 atctttataa gcctgttcat caccagtgaa accagttgaa gataaccatg ttgtttgtga
    16501 aatcttagtt ttatctaaag cagagcgtat cggtcttttc gagctataag gcaaatcttg
    16561 gatttttcct gtcgaaattg cgtgaattga tatcatatca ttctccttaa cgagtattat
    16621 ttaaaatata ttgtactata aaacattttt agaattgtaa attctgattg atatgtttaa
    16681 aaaaatgtta gaaataaggg tatgttaatt tatacgagtc atgtaaaata tgaattgaat
    16741 agattacata aatatgggag gctaccttca tgaaagatgt caaagcactc aagttaatga
    16801 cattaaatga tgttttaagt caaatcaatg gtgacatgac gctgggaatt ggtacaggaa
    16861 gtacaatgga attactctta cctcaaatgg cgcaactaat taaagaacgt ggttacaata
    16921 ttacaggtgt atgcacttct aataaaattg catttttagc aaaagaatta ggtattaaaa
    16981 tatgtgaaat caatgatgtt gatcatatcg acttagcaat tgatggtgct gatgaagtag
    17041 atccatcttt aaatataatt aaaggtggcg gtggtgcgct gttcagagag aaagttatag
    17101 atgaaatggc atcgcgtttt gttgtggttg tcgatgaaac gaaaatagtt caatacttag
    17161 gtgagacgtt taagttacca gtagaggtgg ataaatttaa ctggtatcac atattacgta
    17221 aaattgaatc atacgctgat ataaaagtag aacgtcgtgt aaacgaagat gtcgcattta
    17281 taactgataa tggcaattat attttagatt gtaagttacc aaaaggaatt gacccatata
    17341 agttccatga gtatttaatt catctgacag gtgtgtttga gacaggatac ttcttagata
    17401 tggcagatca agttattgta ggcactcaag agggtgttaa aattttagaa aaataatata
    17461 tagtatagaa aagcgcttgc tagcatcatc tttttaagag gtactagcaa gcgctttgtt
    17521 attatttgtt tttatctgaa gattgttctt cagttttttc gatatgtttt tctgcatcaa
    17581 ttacaactga tgaacgtctg tcttctttat aagtcgcagg ttcttttaat gattgctcta
    17641 tttcatcttc atttgaatcg tttgttaaat cgtcactttg tggtgcttgt ttaactgatt
    17701 ctgctgattc tactgactcc gaagtgatgt catgatcttc atcatgatta gattcagttt
    17761 gattacctat gtgatgttga ggttctgttt tttcagtacc ttcatgaatg cttggttcgt
    17821 cagccactgt attttcagta tcatttttag caacaccaac cgttgatgca gtagctacac
    17881 ctgttttttc agcatcttta atatttgaag aagatttttc agcctcagta tgattagttt
    17941 cttcatcgtc attgacatgg tctaaataat tattaggctc aagttcttca aggtttcgtc
    18001 ttgttgttaa atatgcaata cctcggataa ttaagcttaa accaatgtat cctactgcaa
    18061 cgattgctgt tgaaatggcg atgactttga ttgaaaatag atcgccgatt tcttcgctaa
    18121 acaagaaaat aagtccgaat gtcattgaag catggaatgt cgttgcaata taaattgtac
    18181 gtcctttagt cgctctaatt aattcaccaa gaatcataga gaatgagaat gtataaagga
    18241 agttataagc agcaaattct gtaccataag ttgtatttgc tgagaaaact gaatacatca
    18301 aaccaacaac aatacttgca aagaacgtgt tcattttcgt ttcaacaata ttttgtaagt
    18361 atgaacggaa tccgaattct actacgaacg ccatcagaat atgtccaatc agaatgtgtg
    18421 taataggtac tgataagcct gttgattgta ataaaataaa gctatctgca aatgtattaa
    18481 agctgtacat accaataatt agaataatta aaggtaaaat taaagctagc aatagacgtt
    18541 caattacttt caggctgatt gagaatttta aacctgcaag ttggaccttt ttatatttga
    18601 aaacgagtat acaaatgatt gcagcaatta atggtgctag atctgtaact tcaaatataa
    18661 agtgtttgac accaattata gactggaaat ctctgagcat aatggataac gccattgtaa
    18721 tgacaaagaa gacgaaaatc gtcattgccc attgaaaacc agaaatttta ttgttcttca
    18781 ttatatgtaa cctccattag gtaacaagca aaatatcttt tagtaacaat tatacatatt
    18841 acaagccgag agataaattg tctatgtcaa tattaatgaa aatgtaatag tcttgacgta
    18901 ttttattaac ataaatcagc taagtattcg ttattacata gaaaataggg gtaaaagtaa
    18961 tgtagataag tatttttaag atacggattt aactatgaat tgaattcaaa atttcctata
    19021 cgttatactt taattgttaa taagccacat aaataagagg gggaaatgct gtgtacaagc
    19081 aaggtgaacc aaatttatgg actggaaggt tagatagtga aacagacccg aaaaaattta
    19141 gacattttca aacagtaaca tttgaagatt tgtctaagct ggaaaagagt agtatgccat
    19201 caggggtcgg tatattaggc tatgctgttg acaaaggtgt tgctttaaac aaggggcgca
    19261 ttggtgcaaa agaaggacca gatgcgatta aacaagcatt tgcaggtttg ccggatttga
    19321 atcaatgtga aactttagtc gattacggaa atgtttatca tgatcatgag gaattaattg
    19381 atactcaaaa agaatttgct atgcttgcag cgaagtcaat tgctaatcat agacaaacat
    19441 ttttattagg tggtggacat gatattgcgt atgctcaata tttagcaaca cgtaaagtct
    19501 atccaacaca atctattggt gtgataaata ttgatgcgca ttttgataca cgtgctgaac
    19561 aacaatctac atctggaacg agctttagac aaattttaga agaagatgaa aacacagatt
    19621 atttagtgct tggtattgct caaggtggta atacgcaaag tttatttgat tatgctaaag
    19681 agaaaaagat tgattatgtc tttgcagatg aattattgag tcacgtatca ccaacaatta
    19741 aagatatgat tgaacgtttt gtacatgaac atgatgtcat tatgtttacg atttgtatgg
    19801 atgtcattga tagtgcgttt gcgcctgggg taagtgcgcc ggcagtgtta ggtttgtatc
    19861 cacatactgt tcttgaatta gcaaaacgta ttattccaag tgataaggtg tcttcagtta
    19921 gtattgctga gatgaatcca acatatgacg ctgacaatag aactgctaag ttcgttgcta
    19981 atttagtgca tcatttttta aaataattaa aaatatttat atgtgacgag atttgcattg
    20041 cagggcagaa tgtgagtatt tcttaataga agttatatta acgttgatat atttgaaaaa
    20101 taattgcagt aagaagactc tta
//
LOCUS       DAOBBH010000031        19901 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.031, whole genome shotgun sequence.
ACCESSION   DAOBBH010000031 DAOBBH010000000
VERSION     DAOBBH010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19901
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.031"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(135..599)
                     /gene="bstA"
                     /locus_tag="P6L01_000817"
     CDS             complement(135..599)
                     /gene="bstA"
                     /locus_tag="P6L01_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HDP3029902.1"
                     /translation="MTTKTVFDVIDMGLGYLVNVYDAWKVEKVLDDYHEPFSNTIHWQ
                     FGHVLTIFESALAVAGKENIDLNIYRPLFGNGSSPDEWKDEVPSIEKILEGLQTLPER
                     ARNLTEDDLAIELKQPIVGCNNLEELLVLNAIHIPLHAGKIEEMSRILKNLK"
     gene            766..1104
                     /locus_tag="P6L01_000818"
     CDS             766..1104
                     /locus_tag="P6L01_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDP3029903.1"
                     /translation="MSVSDQIMKGLLDGAILGLIAQGETYGYEIMTKLKNQEFPEISE
                     GSIYPVLMRLNKKGYVDTTIKKSSNGGPRRKYYTITDSGLKELKVFKSKWQALNKGMI
                     NLFGGENDVN"
     gene            1094..1861
                     /locus_tag="P6L01_000819"
     CDS             1094..1861
                     /locus_tag="P6L01_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029904.1"
                     /translation="MLTKQSQDFLEKLRVELLFRGKTEAEVEELLEELEDHLTMAEKN
                     NEDTSSIINTPIKSMADQLSPEISLTTGLYKYITLYFSFILAVIIIPRFLDLGTFDVT
                     IAFLLYIASIVILGVVIGMLILRNTLVRFGDQKITYVINVTYGIVVFLWMIFGGLIIK
                     KYPIYSFFELSDKQSFIVGLVLLIALIVICILIKQKIYALIVLVISLPSLIARIAAIF
                     SKEPDTFNLVSVSILIILNIAFIIYTFVNFRKLKKED"
     gene            complement(1980..2618)
                     /gene="pcp"
                     /locus_tag="P6L01_000820"
     CDS             complement(1980..2618)
                     /gene="pcp"
                     /locus_tag="P6L01_000820"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDP3029905.1"
                     /translation="MHILVTGFAPFDNQNINPSWEAVTQLEDIIGTHTIDKLKLPTSF
                     KKVDNIINKTLASNHYDVVLAIGQAGGRNAITPERVAINIDDARIPDNDDFQPIDQAI
                     HLDGAPAYFSNLPVKAMTQSIINQGLPGALSNSAGTYVCNHVLYHLGYLQDKHYPHLR
                     FGFIHVPYIPEQVIGKPDTPSMPLEKIVAGLTAAIEAISNDEDLRIALGTTE"
     gene            complement(2770..3726)
                     /locus_tag="P6L01_000821"
     CDS             complement(2770..3726)
                     /locus_tag="P6L01_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDP3029906.1"
                     /translation="MNYQVLLYYKYTTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGI
                     NGTLSGTKEETEQYMAHMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEDD
                     VDPRHTTGQYLSPVEFRKALEDDDTVIIDARNDYEFDLGHFRGAIRPDITRFRDLPDW
                     IKENKALFTDKKVVTYCTGGIRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGQY
                     WDGKMYVFDDRISVDINQVEKTIIGKDWFDGKPCERYINCANPECNKQILVSEENETK
                     YLGACSYECAKHERNRYVQANNISDNEWQQRLTNFDDLHQHA"
     gene            3970..4941
                     /locus_tag="P6L01_000822"
     CDS             3970..4941
                     /locus_tag="P6L01_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HDP3029907.1"
                     /translation="MSQQDLPTLFYSGKSNSAVPIISESELQTITAEPWLEISKKGLQ
                     LEGLNFDRQGQLFLLDVFEGNIFKINPETKEIKRPFVSHKANPAAIKIHKDGRLFVCY
                     LGDFKSTGGIFAATENGDNLQDIIEDLSTAYCIDDMVFDSKGGFYFTDFRGYSTNPLG
                     GVYYVSPDFRTVTPIIQNISVANGIALSTDEKVLWVTETTANRLHRIALEDDGVTIQP
                     FGATIPYYFTGHEGPDSCCIDSDDNLYVAMYGQGRVLVFNKRGYPIGQILIPGRDEGH
                     MLRSTHPQFIPGTNQLIICSNDIEMGGGSMLYTVNGFAKGHQSFQFQ"
     gene            complement(5092..5607)
                     /locus_tag="P6L01_000823"
     CDS             complement(5092..5607)
                     /locus_tag="P6L01_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HDP3029908.1"
                     /translation="MTNFTFDGAHSSLEFQIKHLMVSKVKGSFDQFDVAVEGDINDFS
                     TLKATATIIPSSINTKNEARDNHLKSGDFFGTDEFDKITFETKSVTENKVVGDLTIKG
                     ITNEETFDVEFNGVSKNPMDGSQVTGVIVTGTINRENYGINFNQALETGGVMLGKDVK
                     FEASAEFSISE"
     gene            5986..6834
                     /locus_tag="P6L01_000824"
     CDS             5986..6834
                     /locus_tag="P6L01_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HDP3029909.1"
                     /translation="MGNYLVLQSDFGLSDGAVSAMYGVAYTVSDDIRVENLTHDIPPY
                     DIWVASYRLYQTVKYWPKGTVFVSVVDPGVGSDRRSIACLTYSGHYIITPDNGSLSHI
                     KHYEGIKKVIEIDEVKSRLPHMEESHTFHGRDVYAYNGARLAANKIAFERLGQAINVD
                     SIEALEIREATKNEDSVTGSIDILDIRFGSLWTNIPLAFLKSLEIVHGNNLVVTIYHQ
                     DKKVYQNIIKFARSFADVNVGEPLVYVNSLVNIGVAVNQDSFSDLYHIGTGNDWTIHL
                     SKAPSI"
     gene            6863..7417
                     /locus_tag="P6L01_000825"
     CDS             6863..7417
                     /locus_tag="P6L01_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HDP3029910.1"
                     /translation="MKKQDISVKTVVAIGIGAAVFVILGRFVVIPTGFPNTNIETSYA
                     FLALISAIFGPFAGLMTGLVGHAIKDFTTYGSAWWSWVICSGIIGCLYGWIGLKLNLS
                     SGLFSRKSMIYFNIGQIIANIICWALIAPTLDILIYNEPANKVYTQGVISAVLNIISV
                     GIIGTILLKAYASSQIKKGSLRKE"
     gene            7470..9182
                     /locus_tag="P6L01_000826"
     CDS             7470..9182
                     /locus_tag="P6L01_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3029911.1"
                     /translation="MTEPIISFKDFSFQYHSQATPTLQNINVDIYPGEKVLVVGASGS
                     GKSTFANCINGLIPFKTKGNITGELYINNQDATVSCLHDRSNVVGTVLQDTDGQFIGL
                     TAAEDMAFLLENNCVEQDDMKKNVSYWAEKVGMIEHLNHRPQDLSGGQKQRVSLGGIL
                     IHRTPILILDEPLANLDPATGHETLRLLNNIHEETKSTMIIVEHRLEESLDDTFDRVL
                     LFKDGKIIANTTPSDLLKSSKLKEAGIREPLYCTALKYAEVDVESIDNLANLRDVCMS
                     EHVKFKVKKWIDETSANNDNKYKSEPLLELNEVCVQYSDYSNSVLNNVQLNVYRREML
                     SIVGHNGAGKSTLAKAICGFLDITGNIQFCNRGFNQLSISERSEFVGYVMQNPNHMIS
                     EKMIYDEVALGLRARGMKESDIKIRVENVLKICGLYAFRNWPIAALSYGQKKRVTIAS
                     VLVLNPEIIILDEPTAGQDFYHYNEIMSFLIELNRQGKTIIMITHDMHLLSEYSSRTV
                     VLSKGQVVADTTPVLILNDKKICEIASLRQTSLFEMAEYIGISEPQKLVQLFINHDRK
                     VRRQ"
     gene            9179..10012
                     /locus_tag="P6L01_000827"
     CDS             9179..10012
                     /locus_tag="P6L01_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDP3029912.1"
                     /translation="MNQYNTIGFHPGNSRIHQLNATVKLLFLLVVSISAMVTYDTRYL
                     ILISASSILLFKYAHIEWKQVRFVVKFILFFTILNIIAVYIFDPEYGVKIYNQRTELV
                     NGIGRFTLTSQELFYLFNLILKYISTVPLALIFLFTTNPSHFAASLNQLGVNYKISYA
                     VSLALRYIPDIQETYFNISQAQQARGYDNSKKAKFTSRVKGIKRIVLPLIFSSIERID
                     TISTAMELRQFGQYKRRTWYVKKQLKKDDYVVLCLTLILLMLVVTLFFLNNSRYFNPW
                     H"
     gene            10059..10556
                     /locus_tag="P6L01_000828"
     CDS             10059..10556
                     /locus_tag="P6L01_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDP3029913.1"
                     /translation="MINTERLNLMIPSSSHLIELYNICSHPQANIYTPKGLHNSKLDT
                     QRWIEKWQNHWQQYQFGYFVLVKKIDCSVIGICGYEYRQLKQETVLNLFYKLHPSFEG
                     QGYACEAITAITNFVNYIDQETVKVIRTNKCNQRSINLAERLKFKRDKAMDDIINQGD
                     IVFYK"
     gene            10674..11798
                     /locus_tag="P6L01_000829"
     CDS             10674..11798
                     /locus_tag="P6L01_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDP3029914.1"
                     /translation="MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAKHKSK
                     MDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKN
                     NYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDK
                     NKHVVSETTDKKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYR
                     TNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLEGIKRDTKRWKDE
                     VEPIVGKTDMFVFPHGAQDRHTQAYDYLVDEAEFKFIAGVGPNNFTDISATNVYQDRV
                     AIDGLNLFEFKYKLKPFFNPENVYSKQDRQFFKGNRDYEE"
     gene            12102..12920
                     /locus_tag="P6L01_000830"
     CDS             12102..12920
                     /locus_tag="P6L01_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HDP3029915.1"
                     /translation="MNNSEQLIALKESETAFLKYFNKADYELVDFSVVEKLDWKQLNH
                     EDLQQMGERNFWQHEHQIYALRNDFTDQLLRYYSMYPTAATKVAYTGLIIRNNEAAVQ
                     VGLENYAPSLANVQQSLKLFIQFIQQQLRDNVHFVVLGHYQLLDALLDKSLQTPDILS
                     MIEERNLSGLVTYLSTEHPIVQILKENTQQQLNVLEHYIPNDHPALVELKIWERWLHT
                     QGYKDIHLDITAQPPRSYYTGLFIQCHFAENESRVLTGGYYKGSIEGFGLGLTL"
     gene            12938..13552
                     /locus_tag="P6L01_000831"
     CDS             12938..13552
                     /locus_tag="P6L01_000831"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDP3029916.1"
                     /translation="MLRIAIAKGRLMDSLINYLDVIEYTTLSETLKNRERQLLLSVDN
                     IECILVKGSDVPIYVEQGMADIGIVGSDILDERQYNVNNLLNMPFGACHFAVAAKPET
                     TNYRKIATSYVHTAETYFKSKGIDVELIKLNGSVELACVVDMVDGIVDIVQTGTTLKA
                     NGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA"
     gene            13545..14795
                     /locus_tag="P6L01_000832"
     CDS             13545..14795
                     /locus_tag="P6L01_000832"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDP3029917.1"
                     /translation="MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYN
                     LTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESV
                     ECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVL
                     AACYITQVNQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGID
                     QIAGPTEIALIIDDTADLDAIVYDVFAQAEHDELARTYVIGEDAQVLKDLESRIAKAL
                     PNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVG
                     ALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIA
                     DSAQHIAHVEALYNHQQSILIRQS"
     gene            14811..15824
                     /locus_tag="P6L01_000833"
     CDS             14811..15824
                     /locus_tag="P6L01_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HDP3029918.1"
                     /translation="MIYIDKNESPVTPLDEKTMTSIISATPYNLYPDAAYEQFKEAYA
                     KFYGLSPEQIIAGNGSDELIQKLMLIMPEGPALTLNPDFFMYQAYAAQVNREIAFVDA
                     GSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTAFLTAIADKMKALNGYFVID
                     EAYLDYGTAYDVELAPHILRMRTLSKAFGIAGLRLGVLISTAGTIKHIQKIEHPYPLN
                     VFTLNIATYIFRHREETRVFLTMQRQLAEQLKQIFDTHVADKMSVFPSNANFVLTKGS
                     AAHQLGQYVYEQGFKPRFYDEPVMKGYVRYSIATASQLKQLEEIVKEWSAKYDLSKTT
                     KHS"
     gene            15793..16371
                     /gene="hisB"
                     /locus_tag="P6L01_000834"
     CDS             15793..16371
                     /gene="hisB"
                     /locus_tag="P6L01_000834"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HDP3029919.1"
                     /translation="MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFH
                     SGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGTMYIPMDETLAR
                     VVVDISGRPYLSFNASLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHE
                     IEAIFKAFSRALGIALTATDDQRVPSSKGVIE"
     gene            16368..16946
                     /gene="hisH"
                     /locus_tag="P6L01_000835"
     CDS             16368..16946
                     /gene="hisH"
                     /locus_tag="P6L01_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDP3029920.1"
                     /translation="MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILP
                     GVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNI
                     SRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQF
                     NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND"
     gene            16939..17643
                     /locus_tag="P6L01_000836"
     CDS             16939..17643
                     /locus_tag="P6L01_000836"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDP3029921.1"
                     /translation="MIELWPAIDLIGSTSVRLTEGKYDSEEKMSRSAEESIAYYSQFE
                     CVNRIHIVDLIGAKAQYAREFDYIKSLRRLTTKDIEVGGGIRTKSQIMDYFAAGINYC
                     IVGTKGIQDTDWLKEMAHTFPGRIYLSVDAYGEDIKVNGWEEDTELNLFSFVRQLSDI
                     PLGGIIYTDIAKDGKMSGPNFELTGQLVKATTIPVIASGGIRHQQDIQRLASLNVHAA
                     IIGKAAHQASFWEGLE"
     gene            17640..18398
                     /gene="hisF"
                     /locus_tag="P6L01_000837"
     CDS             17640..18398
                     /gene="hisF"
                     /locus_tag="P6L01_000837"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDP3029922.1"
                     /translation="MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAIYYNEAGA
                     DELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSL
                     NSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTHGGKKMTNIKVYDWVQ
                     QVEQLGAGELLVTSMGHDGMKQGFDIEHLAKIKSLVNIPIIASGGGGNAQHFVELFNQ
                     TDVSAGLAASILHDRETTVQSIKEVIRQGGIAVR"
     gene            18395..19027
                     /locus_tag="P6L01_000838"
     CDS             18395..19027
                     /locus_tag="P6L01_000838"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDP3029923.1"
                     /translation="MTKYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGV
                     VCFYSRSKQRLWTKGETSGHTQLVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTE
                     VPFSVQTLAQTVQDSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKA
                     FVSEVADELYHLFVLMHALGVDFSEIEAELARRHHKRNNFKGERQNIEQW"
     gene            19220..19399
                     /locus_tag="P6L01_000839"
     CDS             19220..19399
                     /locus_tag="P6L01_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029924.1"
                     /translation="MKSKLSNSGIDVTVKDVEKYMNRYNEVMMGKNGEKAKELCLSLL
                     PINIIVVFTFFVFIL"
BASE COUNT         6963 a         2948 c         3692 g         6298 t
ORIGIN      
        1 aaatcattaa tataattatt tttcgagaag cggtgaagaa ctggtataga tggtgtttat
       61 taaattcaaa aaagtggcct tttaaacaaa taaaaaaccc gttaacgtgt tgttaacggt
      121 taataagcac atatttattt taaatttttt aatatacgag acatctcttc aattttacca
      181 gcatgaagtg ggatgtgaat ggcatttaat actaataact cttctaagtt attacaacca
      241 acaattggtt gtttcaattc aattgctaaa tcatcttcag ttagatttcg tgcacgttca
      301 ggtaatgttt ggagaccttc taaaatcttt tcaatactcg gtacttcatc cttccattca
      361 tctggagacg aaccatttcc gaataaaggt ctatagatat ttaaatcaat attctcttta
      421 ccagcaacag ctaaggccga ttcaaaaatt gttaatacat gcccaaattg ccaatgaatg
      481 gtattagaaa aaggctcatg ataatcatca agtacctttt caactttcca agcatcatac
      541 acatttacta aatatcctaa ccccatatca atgacatcaa atactgtttt tgtagtcatt
      601 attaattcct cccccataaa taaaacatac ttccgtaagt attaccctat taacaaagtt
      661 gttagtcatt taaggaatgt gtacaacaac taccttacaa taaaagatag ttgacgaaaa
      721 gttctcaatg tactatctta ttatgtaaga tagtaggtga ttaaaatgtc agtgtcagat
      781 cagattatga aaggtctctt agatggggcg atattaggtc tcattgccca aggcgaaacg
      841 tatggatatg aaattatgac gaaacttaag aatcaagagt ttccagagat aagtgagggt
      901 agtatttatc cagttttaat gcgtttaaac aaaaaaggat acgttgatac gacaataaaa
      961 aaatctagta atggcggccc gaggcggaaa tactatacga ttaccgattc aggtttaaag
     1021 gaactaaaag tatttaaatc aaaatggcaa gctttgaata aagggatgat taacttgttt
     1081 ggaggggaaa acgatgttaa ctaaacaatc tcaagatttt ttagaaaaat tacgtgttga
     1141 attgttattt agaggtaaga ctgaagcaga agtagaagag ttattggaag agctcgaaga
     1201 tcatttaact atggctgaaa aaaataacga agatacaagt tcaattatta atacaccgat
     1261 taagtcaatg gctgatcaat tatcacctga gataagtctt actaccggtt tgtataaata
     1321 tattactttg tacttctctt ttatattagc agtcattata attcctaggt ttttagattt
     1381 gggtacattt gatgtgacaa ttgcattttt actttatata gctagtatcg ttattttagg
     1441 tgttgtcatt ggtatgctga ttctacgcaa tacattagtc cgatttggtg accaaaaaat
     1501 aacgtatgtt ataaatgtga cttatggaat tgtcgtattt ttatggatga tttttggtgg
     1561 tttgattatt aaaaaatatc ctatctatag tttctttgag ttaagtgaca aacagagttt
     1621 catcgttggg ttagtacttt taattgcgct tattgtaatt tgtattttaa ttaaacagaa
     1681 aatttatgcg ctcatcgtgt tggtaatttc attgccaagt ttgatagcaa ggatagcagc
     1741 tatctttagt aaagaaccag acacttttaa tttagtttca gtaagtatac tcatcattct
     1801 aaatatagca tttattattt atacatttgt aaattttagg aagttaaaaa aagaagacta
     1861 aacaatgtcg tttagctaaa atacatcatt atagtaaaac tgtataccaa tcatccaaaa
     1921 gcaccctaaa gttagactta aactaacaaa ttagggtgct tttgtttgtg catttataac
     1981 tattccgttg tgcctagagc tatacgtaaa tcctcatcat tagaaatagc ttcaatggct
     2041 gcagtcaaac ctgcaactat cttttctaat ggcatagatg gtgtatcggg tttaccaatg
     2101 acctgctctg gtatgtatgg cacatgaata aatccgaatc gtaggtgagg gtaatgctta
     2161 tcttgtaaat aacctaagtg ataaagcacg tgattacata catacgtacc tgcgctattt
     2221 gaaagtgctc caggaagtcc ttgattaata atactttgag tcattgcttt aactggtaaa
     2281 tttgaaaaat aagctggcgc accgtctaag tgaatggctt gatcaatagg ttgaaaatca
     2341 tcattatctg gaatacgtgc atcatcaata ttaatggcga cacgttctgg ggtaatggca
     2401 tttctaccac cagcttgtcc tatagctagt acaacatcat aatgattaga tgccaacgtt
     2461 ttatttataa tattatctac tttcttaaaa gaggttggta gttttaattt atcgattgta
     2521 tgtgtgccaa taatatcttc tagttgagtc acagcttccc atgagggatt gatattttga
     2581 ttgtcaaaag gcgcaaaccc tgttactaaa atgtgcattt taagacaccc cgcagtatta
     2641 aaatttaatt tagtataaca cgcgcataat taaaaacata tcaggttatg tatgcgcata
     2701 aaaaacagcc ttgaaaaacc tttataaatc ggattttcaa agcgtgtttt gaaaggtatt
     2761 taattaaaac taagcatgtt gatgtaaatc atcaaagttt gttaaacgtt gttgccactc
     2821 attatcacta atattatttg cttgaacata acgattacgc tcatgtttag cacattcata
     2881 agagcatgca cctaaatatt tagtttcgtt ttcttcagaa actaatattt gtttattaca
     2941 ttctgggtta gcgcaattaa tataacgttc acatggtttg ccatcaaacc aatccttacc
     3001 aataattgtt ttttcaactt ggttgatatc aacactgata cgatcatcaa atacatacat
     3061 tttaccgtcc caatattgac cttttgtttc agggtcttta ccatatgtag ctataccgcc
     3121 gtgaagctgc gctacatctt cgaaaccttc ttttaaaagc catccagaaa atttttcaca
     3181 tcgaatgcca cctgtacagt acgtaaccac ttttttatct gtaaataacg ctttattctc
     3241 tttaatccag tcaggtaaat ctctaaagcg tgtgatgtct gggcgaattg cacctcggaa
     3301 atgacctaaa tcaaattcat aatcattacg tgcatcaata atgactgtgt catcatcttc
     3361 aagagctttt ctaaattcta caggtgataa atattggcca gttgtgtgtc ttggatcgac
     3421 gtcatcttct aaatctaaag caacgatttc ttttcgagga cgtacatgca ttttcttaaa
     3481 agcatgtcct tcagcttcat caattttaaa caccatatct ttgaatcgtt catcggcatg
     3541 catatgtgcc atatattgtt cggtttcttc ttttgtacca gataatgtgc cgttaatacc
     3601 ttctgtagaa acaagaattc taccttttaa atggtgtgct ttgcaaaacg ctaagtgatc
     3661 ctgagcaaac tgttcagggt catcaatcgt cgtatattta taatataaaa gaacttgata
     3721 gttcataggt taatctcctt ctataaacat gttataagca tgttaatttt taaaataaag
     3781 cgtcaatacg catatttatc tacgtccatc attttaacag agttgtattg tcattcaata
     3841 tttctagctt gaaatcagcc actagaccga taaaatagat agatgaaacc gttatcattt
     3901 tatttgatag ttatcttgtt taaaataaaa ttgatagtta aaacgaatga aatgaaagga
     3961 atgatcatga tgtcacaaca agatttacct acattatttt atagcgggaa gtccaatagt
     4021 gctgttccaa ttatatctga aagtgaatta caaacaatta cagctgaacc atggcttgaa
     4081 atttccaaaa aaggattgca actagaagga ttgaactttg atcggcaggg acaactcttt
     4141 ttattggatg tattcgaagg caatattttc aaaatcaatc ctgaaacgaa ggaaatcaaa
     4201 cgaccttttg taagtcacaa agcgaatcct gcagcaatca aaatacataa agatggccga
     4261 ttattcgttt gttatttagg agattttaaa tctacaggag gcatttttgc agctacagaa
     4321 aatggtgaca acttacaaga tattattgaa gatctttcaa cagcatattg tattgatgac
     4381 atggtatttg attctaaagg tggattttat tttacagatt ttagaggata ctctaccaat
     4441 ccactaggag gcgtttatta tgtttcgccg gactttagaa cagtgacgcc tatcattcaa
     4501 aatattagcg tagcaaatgg tattgcttta agtacagatg aaaaagtact atgggtaaca
     4561 gaaactacag ccaatcgatt acatcgcatt gcacttgaag atgatggtgt gacgatacaa
     4621 ccatttggag ctactatacc gtactatttt acaggtcatg aaggaccaga ctcatgttgt
     4681 attgatagtg acgataattt atacgtagca atgtatggtc aaggtcgagt gttagttttt
     4741 aataaaaggg gttatccaat aggacaaata ttgataccag gccgagatga agggcatatg
     4801 ttacgttcta ctcatccgca atttatacct ggaacaaatc aactcatcat ttgttccaat
     4861 gatatagaaa tgggcggagg atctatgctt tatacagtta atggctttgc aaaaggtcat
     4921 caaagttttc agtttcaata agacctacct gaatgaaagg cgtcttaaat tagggtgaac
     4981 agttagtgct tgttacacat gagttaaacg aaaaatgcca actataccta gggatgatat
     5041 agttggcatt tcgtatatat atattaaaac aatgaggatt gtgtaatgag attattctga
     5101 gattgagaat tcagctgatg cttcgaattt aacatctttg cctagcatta cgccaccagt
     5161 ttcaagtgct tggttaaagt taatgccgta gttttctcta ttgattgtac cagtaacaat
     5221 aacacctgtt acttgagaac catccatagg attcttactt actccgttga attcaacgtc
     5281 gaatgtttct tcgttagtga tacctttaat tgttaaatca ccaacaactt tgttttcagt
     5341 aactgatttt gtttcaaacg tgattttatc aaattcatca gtaccaaaga aatcaccaga
     5401 ttttaagtgg ttatcacgtg cttcgttttt agtgttaatt gagcttggaa taattgttgc
     5461 agtagctttc aaagtactga agtcattaat atctccttca acagctacat caaattgatc
     5521 aaatgaacct ttcactttag aaaccattaa atgtttaatt tggaattcta aactactgtg
     5581 tgcaccatca aaagtaaagt tagtcataat ataaaacctc catattttta tctcttagat
     5641 atatttttta tacttgatat acatacaata ccacccaaaa aacaaagatg caagcatttt
     5701 atttcgaatt cgaatatttt ctgtgatgta tttaaagaat gacctataaa acaccacact
     5761 attgttatac cctagtcaat taattcaaac ataaagaagt gattacaagt ccgtgaaatt
     5821 tagttaatga gataaatctg actattatta tcatttttat agtcactact attgactttg
     5881 catcaagtga ctcgtactat tttaatataa ttctgaaaat tcaaaatata gaacgttaaa
     5941 tatgtagatt atattttttt gaagttttaa aaaaggaggc attttatggg aaattatttg
     6001 gttttgcaat cagattttgg acttagtgat ggcgctgtaa gtgcaatgta tggagttgca
     6061 tatactgtaa gtgatgatat acgtgtcgaa aacttaacgc atgatatacc accgtatgac
     6121 atttgggtag cgtcatatcg tttatatcaa actgtaaaat actggcctaa aggcacagtt
     6181 tttgtatcag tggtagaccc gggtgtaggt agtgataggc ggagcattgc ttgcttaaca
     6241 tattcaggtc attacattat tacacctgat aatggttctt taagtcatat taaacattac
     6301 gaaggtatta aaaaagttat cgaaattgat gaagtgaaaa gccgattgcc tcatatggaa
     6361 gaaagtcata cttttcatgg tagagatgtt tatgcataca atggtgcgag attagctgca
     6421 aataagatag cgtttgaacg tctcggtcag gcaattaatg ttgatagtat tgaggcatta
     6481 gaaataagag aagcgacaaa aaatgaggat tctgttacag gaagcattga tattttagat
     6541 atcagatttg ggtcattatg gacaaatatt ccattggcat tcttaaaatc cttggaaatt
     6601 gttcatggta ataatttggt ggtaacaatt tatcatcaag ataagaaagt ctatcaaaat
     6661 attattaaat ttgcgcgatc gtttgcagat gttaatgtgg gtgaacctct tgtgtatgtt
     6721 aactcgctag taaatattgg tgtagcagtc aatcaagact cattttcaga tttatatcat
     6781 attggtactg gaaatgattg gacgattcat ttatctaaag caccatcaat ttaatgaatt
     6841 ttttatgtaa ggagtaatga agatgaaaaa acaagatatt tcagttaaaa ctgttgttgc
     6901 gattggtata ggagcagcgg tatttgtgat tttagggcgt tttgttgtaa taccaacagg
     6961 ttttcctaat acgaatatag aaacatctta tgcattttta gcattaatat ctgcaatatt
     7021 tgggcctttt gctggtttaa tgactggatt agttggacac gctattaaag atttcacgac
     7081 atacggtagt gcttggtgga gttgggttat ttgttcagga attataggct gtttatatgg
     7141 atggattggc ctaaaattaa atctttcttc aggcctattt tctaggaaat cgatgattta
     7201 ttttaatatc ggtcaaatta ttgcgaatat tatttgctgg gcacttattg caccaacatt
     7261 agatattttg atttataacg aaccggctaa caaggtttat acacaaggtg ttatctctgc
     7321 agtattaaat attatttcag ttggtattat tgggacaata ttattaaaag catatgcttc
     7381 atctcaaata aaaaaaggta gtttacgtaa agaataatca ttttgttgaa tcagatatgt
     7441 aaatgaatgt agaaaggtaa tgatatatca tgactgaacc aattatctcg ttcaaagact
     7501 ttagttttca atatcatagt caagcaacac ctacattaca gaatataaat gttgatattt
     7561 atccaggaga aaaagtgcta gtagttggtg cttcaggtag tggtaaatcg acttttgcaa
     7621 attgcataaa cggattaatt ccatttaaaa ctaaaggtaa cataaccggg gaactatata
     7681 taaataatca agatgcaacc gttagttgtt tacatgatag atctaatgtt gttggtacag
     7741 ttttacaaga tacagatgga cagttcatag gcttaacagc agctgaagat atggcctttt
     7801 tattagaaaa taattgtgtt gaacaagatg atatgaagaa aaatgtaagt tattgggctg
     7861 aaaaagttgg catgatagaa catttaaatc accgaccgca agatttatct ggaggtcaaa
     7921 aacaacgcgt ttcattaggt ggtatattaa tccatcgtac gcctatttta atattggatg
     7981 agccgctggc caatttagat cctgcgacag gacatgaaac gctgagattg ttaaacaata
     8041 ttcatgaaga aacaaagtca acgatgatta ttgtcgaaca tcgattagaa gaatcattag
     8101 atgatacgtt tgatagagtt cttcttttta aagatggaaa aatcatcgct aatacaacgc
     8161 ctagtgattt gctaaaatca tctaagctta aagaagcagg catacgtgaa ccattatact
     8221 gtacagcgct taaatatgca gaagtagatg tcgaatccat tgataactta gcgaatttac
     8281 gtgacgtttg tatgagtgaa catgttaaat ttaaagtgaa aaaatggata gatgaaacat
     8341 ctgcaaataa tgataataag tacaaatctg aaccgctatt agaattaaat gaggtatgtg
     8401 ttcaatatag tgactatagt aatagtgtac ttaataatgt tcaattaaat gtttatagaa
     8461 gagaaatgct tagtatagtt ggtcacaatg gtgccggcaa atcaacactt gctaaagcaa
     8521 tttgtggatt tttagatata acaggtaata tccaattttg taatagggga tttaatcaat
     8581 tgtcaattag cgaacgatct gaattcgtag gttatgtgat gcaaaatcca aatcatatga
     8641 tttctgaaaa aatgatttac gatgaagtag cattagggtt aagagcacga ggtatgaaag
     8701 aatcagatat aaagatacga gtagagaatg tgctcaaaat atgcggactt tatgcatttc
     8761 ggaattggcc aattgcagct ttaagctatg gtcaaaaaaa gagggtcact atagcatctg
     8821 ttttagtctt aaatccggaa ataatcatat tggatgaacc gactgctggt caagatttct
     8881 atcattataa tgagataatg tcatttttaa ttgagctaaa cagacaggga aagacgatta
     8941 ttatgattac gcatgatatg catttattgt ctgagtatag ttcaagaaca gttgtattat
     9001 caaaaggaca agtcgttgct gataccacgc cagtattgat attaaatgat aaaaaaatct
     9061 gtgagattgc atcattgaga caaacatcgc tatttgaaat ggccgaatat atagggatta
     9121 gcgagccaca gaaattagta caattattta ttaaccatga taggaaggtg agacgccaat
     9181 gaatcaatat aatactatag gttttcaccc gggaaatagt cgtattcatc aattaaatgc
     9241 gactgttaaa cttttattct tattagttgt ttctatttct gcaatggtga cttatgacac
     9301 aagatattta attttaatta gtgcttcatc tattttattg ttcaaatatg ctcatattga
     9361 atggaaacaa gttcgctttg ttgttaaatt cattctgttt ttcacaatat taaatattat
     9421 tgccgtatac atatttgacc ctgaatatgg tgtgaagatt tataatcagc gtacagagtt
     9481 agtcaatggt attggtcgat ttacgctaac atcacaggaa ttattctatc tttttaatct
     9541 aatattaaaa tatattagta cagttccttt agcgttaata tttttattca caacgaatcc
     9601 gagtcatttt gctgcaagtt taaatcagct aggtgtgaat tataaaatca gttacgcagt
     9661 ctcactagca ttaaggtata ttccagatat tcaagaaaca tattttaata tttcacaagc
     9721 gcaacaagca agaggatatg ataattcgaa aaaagcgaaa tttactagcc gtgttaaagg
     9781 tatcaaacgc attgtgttac ctttaatatt ttctagtatc gaaagaattg acactattag
     9841 tactgctatg gagttaagac aattcggaca gtataaaagg agaacctggt acgtcaaaaa
     9901 acaattaaaa aaagatgatt atgttgtttt gtgtttgacg ttaatacttc tgatgttagt
     9961 agttacatta ttctttttaa ataatagtcg atatttcaac ccgtggcatt agtattcata
    10021 taaatagtct ttaaatagaa ataggaggga gacatttaat gataaatact gaaagattaa
    10081 atttaatgat tccaagttcc tcgcatttaa ttgaacttta taatatttgt agtcatccac
    10141 aagcaaatat atacactccc aaaggtttac ataattccaa attagacaca caacggtgga
    10201 ttgaaaaatg gcaaaaccat tggcaacaat atcaatttgg ttactttgta ttggtgaaaa
    10261 aaatagattg tagtgttatt ggtatttgtg gatatgaata tcgacaatta aagcaagaaa
    10321 cagtactaaa tttattttat aaattacatc caagttttga aggacaaggg tacgcatgtg
    10381 aggctattac agcaatcaca aattttgtga attatatcga tcaagaaaca gtaaaagtta
    10441 tcaggacaaa taagtgtaac caacgttcaa taaatttagc agaaaggctt aaattcaagc
    10501 gagacaaagc tatggacgac attatcaatc aaggagatat tgtgttttat aaataaaata
    10561 ctatgacatt atctaaaaaa taaaattaaa atcccaacag tttcttaaaa agttatgagc
    10621 cacataataa aaatgttata attgtgcata ataaaacata gggagaatgt ttaatgcata
    10681 aaaaatattt attgctattt tttctgttat tatcagttgc attgctatat agctgttcca
    10741 agcaaaatga gaaagctcaa aataaatctg ttaatccaaa tgagtatgca aagcataaat
    10801 cgaaaatgga tgaacattgg gaaacatatc acggccaagt ttatcatgtg ttttatcacc
    10861 cattaattac agacccaaaa gttgcgttta caggagatac acaccaagct aagggcaaca
    10921 atgattggat gattactgta agtgaattta aaaaatcatt gaacgaatta tataaaaata
    10981 attacatcat cgttaatcca catgatgtat acgacttatc atctcatcat gtaaaagcca
    11041 aaaaaataaa attgccgaaa ggtaaaaagc cactaatcat ctcaattgat gatatgaatt
    11101 actatagtta catgagacac catggttatg ctgaccgttt agtactcgat aaaaataaac
    11161 atgttgtttc tgaaactaca gacaaaaaag gtcatgctac ttattctgat gacaatgata
    11221 tcgtgccaat tttaaataaa tttgtaaaag agcatccaga tttttcttta aatggtgaaa
    11281 aaggtgttgt tgctttaacc ggttatgagg gcgtattagg ttatcgcacg aatgaattaa
    11341 cgagtaaaga ttatacaaag aataaaaagg cagctgagga agttgttcgc gcaatgaaac
    11401 gggatggatg gtcatttgcg agtcattcat atggtcacat taattttgaa aaaacatctt
    11461 tagaaggtat taaacgtgat actaaaagat ggaaagatga agttgaacca attgtaggta
    11521 aaacagatat gtttgtattt ccacatggcg cacaggatag acatacacaa gcgtatgatt
    11581 atttggtaga tgaagcagag tttaagttta ttgcaggtgt cggtccaaac aattttacag
    11641 atatatcagc aacgaacgtt taccaagata gggttgcgat tgatggtcta aacttatttg
    11701 aatttaaata taaactcaaa ccgtttttta atcctgaaaa tgtatatagt aaacaagata
    11761 gacaattctt taaaggtaat cgggattatg aagaatagtt aaatttaata aaaagataga
    11821 aagaaaataa ctttacataa taaggcaagc acaatatgtg caagaagtac aacttcaata
    11881 taaaaatctt ggtttagcat ttttataggc acaaatgtct tgcctttttc ttttgtaaaa
    11941 atttaaaaac aatacaccga attttctgaa aaaggttgta atgtattgct aaaaatgata
    12001 agttattaat catattacac tttagtgtgg tagagaagta aagtaaacaa cgaaaatacc
    12061 aagtgatttt aaatacagta ttgaaagtaa agggtggtcg agtgaataat tcagaacaat
    12121 taattgcgtt aaaagaatcg gaaacagcat ttttaaaata ttttaataaa gcggattatg
    12181 agttagttga ttttagtgtg gttgaaaagt tggactggaa acaattgaac catgaggatt
    12241 tgcagcagat gggtgagcga aatttttggc aacatgagca tcaaatttat gcacttcgaa
    12301 atgattttac agatcaatta ttgcgttatt actcaatgta tccaactgca gcaacaaaag
    12361 tggcatacac aggtttaatc attcggaata atgaagcggc tgtacaagtg ggtttggaaa
    12421 actatgcacc atcattagcg aatgtacaac agagcttaaa gctatttatt cagtttattc
    12481 aacagcagtt gagagacaac gtgcattttg ttgtattggg acattatcaa ttgcttgatg
    12541 ccttactcga taaatcgtta caaacaccag acatactatc aatgattgag gaacgtaatt
    12601 tatcgggact ggttacatat ttgtcgactg aacatccaat cgtgcaaatc ttgaaagaga
    12661 atacgcaaca acaattaaat gtattggaac actatattcc aaatgaccat ccagcactcg
    12721 ttgaattaaa aatatgggaa cgttggttac atacacaagg ttacaaagac atccatttag
    12781 atattactgc gcagccacct agatcctatt acacaggctt atttattcaa tgtcattttg
    12841 ctgaaaatga atctcgagtt ttaacaggtg gttattacaa aggaagcatc gaagggtttg
    12901 gattaggatt aacactttaa gtaagggagt atgcacaatg ttaagaatcg ccatagccaa
    12961 aggacgtcta atggatagtt taattaacta tttagatgta attgaatata cgacattatc
    13021 agaaacatta aaaaatagag aacgccaatt attattaagt gtagataata ttgaatgcat
    13081 tttagtaaaa ggaagtgacg tgccaatcta tgtggaacaa ggaatggccg acataggcat
    13141 tgttggtagc gacatattag atgagcgcca atataatgtt aataatttgt tgaatatgcc
    13201 ttttggagca tgtcattttg cggttgcagc gaaacctgaa acgaccaatt atcgtaaaat
    13261 cgcaacgagt tatgttcata ctgctgaaac atattttaaa tcaaaaggta ttgatgtcga
    13321 attgattaaa ttgaatggtt ctgttgaatt agcttgtgtc gtagatatgg tagacggaat
    13381 tgtcgacatc gttcaaacag gtactacgct aaaagcgaac ggactggttg aaaagcaaca
    13441 tattagtgat atcaatgcaa gattaataac taataaagca gcttatttta aaaaatcaca
    13501 attaatagag caatttattc gctctttgga ggtgtctatt gccaatgctt aatgcacaac
    13561 aatttttaaa tcaattttca ttagaagcac cattagatga gtcattgtat ccaattattc
    13621 gcgatatttg tcaggaagtt aaagttcatg gagataaggc tttgaaaatg tataatctaa
    13681 cattcgatca tacgaaaaca gatcatttag aaattagtca tgaacaaatt aaagcagcat
    13741 ttgacacatt agatgaaaaa acaaaacaag cattacaaca aagttatgaa agaattaaag
    13801 catatcaaga aagtattaag cagacgaatc aacagttaga agaatcagtg gagtgttatg
    13861 aaatatacca tccactagaa agtgtgggta tttatgtgcc tggtggcaaa gcaagttatc
    13921 catcaacggt tctaatgaca gcgactttag cacaagtagc gggtgtagaa aatattgttg
    13981 ttgtgacacc acctcaacct aacggagtat cccaagaggt attagctgca tgttatatta
    14041 cgcaagttaa tcaagtgttt caagttggtg gtgctcaaag tattgctgca ttgacttatg
    14101 gaacagaaac gatacctaaa gttgataaga ttgtaggtcc aggtaaccaa tttgttgcat
    14161 atgccaaaaa atatttattt ggacaggtag gtattgatca aattgcagga ccaacagaaa
    14221 tagcactgat tattgacgac accgcagatt tagatgccat cgtatatgat gtatttgcgc
    14281 aagcagaaca tgatgaatta gcacgtacat atgtcattgg tgaagatgcg caagtcctta
    14341 aagatttaga atcacgtatt gctaaagcat tgcctaatgt ggacagatac gacattgttt
    14401 ctaaaagtat cgctaatcaa cactacctta tccatgctag taattttgat gaagcatgcc
    14461 atgtcatgaa tacaatcgcg cctgaacatg cgtcgattca aacagtaaat cctcaaccat
    14521 atattgaaaa agtgaaatat gtgggtgcat tgtttattgg acattattcg ccagaggtca
    14581 taggagatta cgttgcaggt ccaagtcatg tattacctac aaatagaaca gctagattta
    14641 ccaatgggtt atcggtcaat gatttcttaa cacggaacac ggtcatccat ttatcaaaag
    14701 atacgtttga acaaattgct gattcagcac aacatattgc tcatgttgaa gcattataca
    14761 atcaccagca gtctatttta atacgtcagt cttaggggag tgtaattgaa atgatttata
    14821 ttgataaaaa tgaaagtcca gttacgccgt tggatgaaaa aacaatgacg tctattatta
    14881 gtgcaacgcc atataattta tatcctgatg cagcatatga acaattcaag gaagcttatg
    14941 ctaagtttta cggattatcg cctgaacaaa ttattgcagg aaatggatct gatgaattga
    15001 ttcaaaagtt aatgctgatc atgccagaag gtccggcatt aacgctaaat cctgattttt
    15061 ttatgtatca agcatatgcg gcacaagtaa atcgtgaaat tgcatttgta gatgcaggat
    15121 cagatttaac gtttgatttg gaaaccattt taacgaaaat cgatgaagta caaccatcat
    15181 tttttattat gagtaatcca cataaccctt caggcaagca atttgatacg gcatttttaa
    15241 cagctattgc agataagatg aaagcattaa acggatactt tgtcattgat gaagcatatt
    15301 tagattatgg tacggcatat gacgtggaac tggcaccaca catcttaaga atgcgtacat
    15361 tatcaaaggc gtttggaatt gccggcttaa gattaggtgt cttaattagt actgctggaa
    15421 cgataaagca tattcaaaaa atagaacatc catatccatt aaatgtattt acgctaaata
    15481 ttgcgactta tatttttaga catagagaag agacaagagt atttttaacg atgcaacgac
    15541 agttagctga gcagttaaaa caaatatttg atacacatgt tgcagataaa atgtcagtat
    15601 tcccatcaaa tgccaatttt gtacttacta aaggttcagc agcgcaccaa ttaggacaat
    15661 acgtatatga acaaggattt aaacctcgct tttatgatga gccggtgatg aagggctatg
    15721 taagatactc aattgcaaca gcatcgcagt taaagcaatt agaagaaatt gttaaagaat
    15781 ggagcgcaaa atatgattta tcaaaaacaa cgaaacacag ctgaaacaca actaaatatt
    15841 tcaatatcag atgaccagtc accatcgcat attaatacag gtgtgggctt tttaaatcat
    15901 atgttaacat tgtttacatt tcatagcggt ctgtcattaa acattgaggc acaaggtgat
    15961 attgacgtag atgatcacca tgtaactgaa gatatcggca ttgtcattgg ccaattgtta
    16021 cttgaaatga ttaaagataa aaagcatttc gttcgttatg gaacgatgta cattccaatg
    16081 gatgaaacat tagcacgtgt cgttgtggat ataagtgggc gcccatacct atcattcaat
    16141 gcatcattaa gtaaagaaaa agttggtacg tttgatacgg agttagtaga agaatttttt
    16201 agagcggtcg taatcaatgc aagattaaca acgcatattg atttaattcg tggaggtaat
    16261 acacaccatg aaattgaagc tatattcaaa gcgttttccc gtgcattagg catagcgcta
    16321 actgcaactg atgatcagcg tgtgccgtca tcgaaaggtg tgattgaatg attgtcatcg
    16381 ttgattatgg attagggaat attagtaatg taaaacgcgc tattgaacat ttagggtatg
    16441 aggtggttgt ctcaaatacc tcaaaaataa tcgatcaagc agaaacaatc atattgcccg
    16501 gtgtcggcca ttttaaagat gcgatgtcag agataaaacg attaaatctc aatgcaatat
    16561 tggctaagaa tactgataag aagatgattg gtatttgttt aggcatgcaa ttaatgtatg
    16621 agcatagtga tgaaggcgat gcatctggat tagggtttat cccaggaaat atttcgcgta
    16681 tccaaacaga atacccagtg ccgcacttag gttggaataa tttagtgagt aagcatccta
    16741 tgttaaatca agatgtttac ttcgtacatt cttaccaagc gccgatgtca gaaaatgtca
    16801 ttgcatatgc gcagtatggg gctgatattc cggcaattgt tcaatttaac aattatattg
    16861 gtattcaatt ccatcctgaa aaaagcggta catatgggtt acaaattttg cgtcaggcaa
    16921 tacaaggggg atttataaat gattgaatta tggccagcga ttgatttgat tgggtcaaca
    16981 agtgtgaggt taacagaggg taaatatgat agtgaagaaa aaatgtcgcg ttcggctgaa
    17041 gaaagtattg cttactatag tcaatttgaa tgtgtgaatc gtattcatat cgtcgacttg
    17101 ataggtgcta aggcacagta tgcccgagag tttgattata ttaagtcatt gaggagatta
    17161 acaaccaaag atattgaagt aggtggtggc attcgtacga agtcacaaat catggactac
    17221 tttgccgcag ggattaatta ttgcatagtt ggaacgaaag gcattcaaga tactgattgg
    17281 cttaaagaga tggcacatac atttccaggt cgtatttatc tgtcagttga tgcttatgga
    17341 gaagatatta aagtgaacgg atgggaagag gacacagagt taaatttatt tagttttgtg
    17401 agacagttat cggatatacc tcttggaggc attatatata ctgatattgc taaagatggc
    17461 aaaatgtccg gacctaactt tgaattaact ggtcaattag taaaggcaac gacgattcct
    17521 gtcattgctt ccggtggtat tagacatcag caagatattc aacgattagc gtcgctaaat
    17581 gttcacgctg ctattatagg aaaggctgca catcaagcat ctttttggga ggggctagaa
    17641 tgattaaaaa acgtatcatt ccatgtttag atgtcaaaga tggtcgtgtc gttaaaggga
    17701 ttcaatttaa aggattaagg gatataggga atcctgttga tttagcaata tattacaatg
    17761 aagcgggtgc tgatgaatta gtatttttag acatctctaa gacggaagag ggtcatagct
    17821 taatgctaga agtgattgaa cagacagcgt cacgcttgtt tatccctctt actgtagggg
    17881 gtgggattca aagtctcgat gatattaccc aattgttaaa tcatggtgca gataaagtat
    17941 cattaaattc aagtgcttta aaaaatccac agctcattaa acaagcgagt gataaattcg
    18001 gtagacaatg catctgtata gcaattgata gctattatga tcctgaaaga aaagcacatt
    18061 attgttgtac gcatggtggt aaaaaaatga caaatattaa agtatatgac tgggtacagc
    18121 aagtagaaca gttaggtgca ggtgagctcc tcgttacaag tatgggacat gatggtatga
    18181 aacaaggatt tgatattgag catcttgcaa aaataaaatc ccttgtaaat attccaatca
    18241 ttgcttctgg tggtggtggc aatgcacaac actttgtaga actatttaat cagacggatg
    18301 tttctgcagg tttagctgca agtatattac atgaccgaga aacgacggtt caatcaatta
    18361 aagaagtgat acggcaaggg ggtatagcag taagatgacc aaatataaaa ttgattttag
    18421 caaaggttta gtgccagcaa ttttacaaga taatcaaaca aaacaagtat tgatgttggg
    18481 ttatatgaat caagaagctt ttgataaaac gatagaagat ggtgtggtat gtttctattc
    18541 gcggtcgaaa caacgtctat ggacaaaagg tgaaacatct ggtcatacgc aacttgttaa
    18601 agatattcat gtagattgcg acaatgatac tattttaatt gatgtcatac caaatggacc
    18661 aacatgtcat acaggcagtc aaagttgttt caacacagaa gttccatttt cagtgcaaac
    18721 attagcgcag acagttcaag atagtgccca atccaataat gaaaagtcat atacaaaata
    18781 tttattaaca gaaggtatag aaaagattac aaaaaaatac ggtgaagaag cttttgaagt
    18841 cgtaattgaa gcaattaaag gtgacaaaaa agcatttgta agtgaagtag cagatgaact
    18901 ttatcattta tttgtcttga tgcatgcgct aggcgtcgat ttttcagaaa ttgaggcgga
    18961 attagcgcgt agacatcata agcgcaataa ctttaaaggt gaacgacaaa atatcgaaca
    19021 gtggtaaagc aagtatggac taagatataa ggaaaaggat catggcttat acacttacaa
    19081 atattgtgga aaacgtgaca ttttcaagtt taaaatacga caccaacata ttttaactat
    19141 gaatgctgtg atggtactaa agttgcgaac tcgttataga taagtagtgg ataatcacaa
    19201 tacgaaatca aaaataatta tgaaaagtaa attgagcaac tcaggaatag atgtcactgt
    19261 taaagatgtc gaaaagtata tgaatcgata taatgaagtt atgatgggaa aaaatggcga
    19321 aaaagctaaa gagttatgtt tgtcgttact acctattaat atcatagttg tctttacatt
    19381 ctttgtattt atactataaa tacaaatata tctagcctga aatagaaatg tcatagccta
    19441 tttaaaagac aatctccatt agaactaaga tgtgcatccc gaaagttaga ctaaaaaact
    19501 aactttatgg ggtgtatttt tatgctaatc atcataaatt cgagattaag ttaaaggtag
    19561 ttcaagagta attaaacaat aaattaaaaa tagtaggata cttactttga gggaagaaaa
    19621 ttaactgtat atatttaatg taggaacaag tattacggtt tatcctgata caatcattgt
    19681 ggatgggatg atatttttag gtttaaaata cgacaccagc aaacataata actgtaatag
    19741 ctcataaatc tctccataca gctaatctaa aaaaataata catcattgga atcaagaccc
    19801 aagcatgtaa atattaaaaa tcacaaaaga tatatgtaaa aaagttacaa tttgcataat
    19861 taaattgtgt ctaattattg actaattaaa ttttgccaaa t
//
LOCUS       DAOBBH010000032        19536 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.032, whole genome shotgun sequence.
ACCESSION   DAOBBH010000032 DAOBBH010000000
VERSION     DAOBBH010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19536
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.032"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            269..1444
                     /locus_tag="P6L01_000840"
     CDS             269..1444
                     /locus_tag="P6L01_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosyl diacylglycerol synthase"
                     /protein_id="HDP3029925.1"
                     /translation="MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHD
                     LFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIK
                     EKPDLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQ
                     DFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGF
                     DTMITDILAKSANAQVVMICGKSKELKRSLIAKFKSNENVLILGYTKHMNEWMASSQL
                     MITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVAS
                     LTNGNEQLTNMISAMEQDKIKYATQTICRDLLDLIGHSSQPQEIYGKVPLYARFFVK"
     gene            1422..2612
                     /locus_tag="P6L01_000841"
     CDS             1422..2612
                     /locus_tag="P6L01_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDP3029926.1"
                     /translation="MQDSSLNNYANHKNFILMLIILFLMEFARGMYILSYINFLPTVT
                     SIAVAITSLAFSIHFIADASTNFVIGFLLKKFGTKIVLTTGFILAFTSLFLVIWFPAS
                     PFVIIFSAMMLGIAVSPIWVIMLSSVEEDKRGKQMGYVYFSWLLGLLVGMVFMNLLIK
                     VHPTRFAFMMSLVVLIAWILYYFVDVKLTNYNTRPVKAQLRQIVDVTKRHLLLFPGIL
                     LQGAAIAALVPILPTYATKVINVSTIEYTVAIIIGGIGCAVSMLFLSKLIDNRSRNFM
                     YGVILSGFILYMILIFTLSMIVNIHIVWIIALAIGLMYGILLPAWNTFMARFIKSDEQ
                     EETWGVFNSIQGFGSMIGPLFGGLITQFTNDLNNTFYFSALIFLVLAVFYGSYFIANR
                     EKAK"
     gene            complement(2725..3234)
                     /locus_tag="P6L01_000842"
     CDS             complement(2725..3234)
                     /locus_tag="P6L01_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcG family protein"
                     /protein_id="HDP3029927.1"
                     /translation="MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIE
                     DGDLDSVIEQVRARINGIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGE
                     DFYGEAEHVFVPHFTIAQGLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKW
                     KVIETFKLA"
     gene            complement(3428..4186)
                     /locus_tag="P6L01_000843"
     CDS             complement(3428..4186)
                     /locus_tag="P6L01_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDP3029928.1"
                     /translation="MSEFKTGKINKHVLYSNILNRDVTISIYLPESYNQLVKYNVILC
                     FDGLDFLRFGRIQRTYESLIKEARIDDAIIVGFHYEDVDKRREEFHPQGSRSHLTIQS
                     VGKEILPFIDSTFSTLKVGNARLLVGDSLAGSIALLTALTYPTIFSRVAMLSPHSDDK
                     VLDKLNQCANKEQLTIWHVIGLDEKDFTLPTNGKRADFLTPNRELAEQIKKYNITYYY
                     DEFEGGHQWKDWKPLLSDILLYFLRKNTDDQHYE"
     gene            complement(4331..5899)
                     /locus_tag="P6L01_000844"
     CDS             complement(4331..5899)
                     /locus_tag="P6L01_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDP3029929.1"
                     /translation="MIEKLVTFLNEVVWSKPLVYGLLITGVLFTLRMRFFQVRHFKEM
                     IRLMFQGEKSPNGISSFQAIAMSLAGRVGTGNIVGVSTAIFIGGPGAVFWMWITAFLG
                     ASSAFIESTLGQIFKRVENNEYRGGPAYYIEYGIGGKFGKIYGIIFAIVTIISVGLLL
                     PGVQSNAIASSMHNAIHVPQWLMGAIVVVILGLIIFGGVRSIANVATAVVPFMAIIYI
                     LMAVIIICINIQEVPALFALIFKSAFGLQSAFGGIVGAMIEIGVKRGLYSNEAGQGTG
                     PHAAAAAEVSHPSKQGLVQAFSVYIDTLFVCTATALIILISGTYNVTDGTVNANGTPH
                     LIKDGGIYVENATGKDYSGTAMYAQAGIDKAFHGSGYQFDPTFSGVVSYFIAFALFFF
                     AFTTILSYYYITETNVAYLTRNQNNQVSSIFINIARVIILFATFYGAVKTADVAWAFG
                     DLGVGLMAWLNIIAIWILHKPAVNALKDYEIQKKRLGNGYNAVYQPDPNKLPNAVFWL
                     KTYPERLKQARAKK"
     gene            6241..7326
                     /locus_tag="P6L01_000845"
     CDS             6241..7326
                     /locus_tag="P6L01_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDP3029930.1"
                     /translation="MLNKVWFRTGIALIMLFILIKLFMEVHEVFTPIATIIGSVFLPF
                     LISGFLFYICLPFQNLLEKVGFPRWASITTIMLALFAIIGLIVAFVAPIIISNINNLI
                     SQTPALQKEAEQIIKFALAQMDKLPEDVTSRITNMVKSMGDGVTNILSNSLQYITSLI
                     STIFLLIMVPFFLIYMLKDHEKFIPAVAKFFKGERKVFFVDLLTDLNFTLKSYIQGQV
                     TVSVILGIFLYIGYSIIDLPYIPLLVLFAGVANLIPFLGSWLSFAPAAILGIIDSPTT
                     FIWVCIITLIAHQLEGNIITPNVMGKSLSIHPLTIIVVILAAGDLGGFTLVLIAVPLY
                     AVLKTVVSNIFKYRQRIIDKANSNVKD"
     gene            complement(7523..8293)
                     /gene="fabI"
                     /locus_tag="P6L01_000846"
     CDS             complement(7523..8293)
                     /gene="fabI"
                     /locus_tag="P6L01_000846"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDP3029931.1"
                     /translation="MLNLENKTYVIMGIANKRSIAFGVAKVLDQLGAKLVFTYRKERS
                     RKELEKLLEQLNQPEAHLYQIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDL
                     RGRFSETSREGFLLAQDISSYSLTIVAHEAKKLMPEGGSIVATTYLGGEFAVQNYNVM
                     GVAKASLEANVKYLALDLGPDNIRVNAISAGPIRTLSAKGVGGFNTILKEIEERAPLK
                     RNVDQVEVGKTAAYLLSDLSSGVTGENIHVDSGFHAIK"
     gene            complement(8571..10415)
                     /locus_tag="P6L01_000847"
     CDS             complement(8571..10415)
                     /locus_tag="P6L01_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HDP3029932.1"
                     /translation="MEFLSLVIVVLAAFLTPIIVNRLNINFLPVVVAEILMGIVIGNS
                     FLNIVERDSILNILSTLGFIFLMFLSGLEIDFKAFKKDKRARQGQNDDESSIPGHLNL
                     ALTVFAFIMIISILLAYVFKWLGLVDDVLLMVIIISTISLGVVVPTLKEMNIMRTTIG
                     QFILLVAVLADLVTMILLTVYGAINGQGGSTIWLIGILVVFTAISYILGVQFKRMSFL
                     QKLMDGTTQIGIRAVFALIILLVALAEGVGAENILGAFLAGVVVSLLNPDEEMVEKLD
                     SFGYGFFIPIFFIMVGVDLNIPSLIKEPKLLIIIPILIVAFIISKLIPVMFIRRWFDM
                     KTTIASAFLLTSTLSLVIAAAKISERLNAISAETSGILILSAVITCVFVPIIFKKLFP
                     VPDEFNRKIEVSLIGKNQLTIPIAQNLTSQLYDVTLYYRKDLSDRRQLSDDITMIEIA
                     DYEQDVLERLGLFDRDIVVCATNDDDINRKVAKLAKAHQVERVICRLESTTDDTELVD
                     SGIEIFSSYLSNKILLKGLIETPNMLNLLSNVETSLYEIQMLNYKYENIQLRNFPFGG
                     DIIFVRIIRNNESIVPHGDTQLRYGDRLIVTGAKEYVDELKQELEFYF"
     gene            complement(10425..11810)
                     /gene="mgtE"
                     /locus_tag="P6L01_000848"
     CDS             complement(10425..11810)
                     /gene="mgtE"
                     /locus_tag="P6L01_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDP3029933.1"
                     /translation="MSMNTDEKERVQEELYDETLLDQYLENDDIDQFRDEFLALHTYE
                     QSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEM
                     SYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHT
                     PVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVI
                     SVNVADDQEDVAQVMRDYDFMAVPVIDYQEHLLGIITIDDILDVMDEEASEDYSRLAG
                     VSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISG
                     MSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYH
                     QPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYF
                     GLATSFMAYLI"
     gene            complement(11831..12685)
                     /locus_tag="P6L01_000849"
     CDS             complement(11831..12685)
                     /locus_tag="P6L01_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDP3029934.1"
                     /translation="MKFKYHISQQETVKAFLARHDFSKKTVSAIKNNGALIVNDEPVT
                     VRKQLMPNDILEIHLPREIPSVNLIPYARKLEVLYEDAFIIIVTKPNNQNCTPSREHP
                     HESLIEQVLYHCQEHGENINPHIVTRLDRNTTGIVIFAKYGHIHHLFSKVNLKKIYTC
                     LVYGKTHTSGIIEANIRRSKDSIITREVASDGKYAKTSYEVINQNDKYSLCKVHLHTG
                     RTHQIRVHFQHIGHPIVGDSLYDGFHDKIHGQVLQCTQIYFVHPINKNNIYITIDYKQ
                     LLKLFNQL"
     gene            complement(12682..13491)
                     /locus_tag="P6L01_000850"
     CDS             complement(12682..13491)
                     /locus_tag="P6L01_000850"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDP3029935.1"
                     /translation="MRYTILTKGDSKSNALKHKMMNYMKDFRMIEDSENPEIVISVGG
                     DGTLLQAFHQYSHMLSKVAFVGVHTGHLGFYADWLPHEVEKLIIEINNSEFQVIEYPL
                     LEIIMRYNDNGYETRYLALNEATMKTENGSTLVVDVNLRGKHFERFRGDGLCVSTPSG
                     STAYNKALGGALIHPSLEAMQITEIASINNRVFRTVGSPLVLPKHHTCLISPVNHDTI
                     RMTIDHVSIKHKNVNSIQYRVANEKVRFARFRPFPFWKRVHDSFISSDEER"
     gene            complement(13508..14143)
                     /locus_tag="P6L01_000851"
     CDS             complement(13508..14143)
                     /locus_tag="P6L01_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDP3029936.1"
                     /translation="MNQWDQFLTPYKQAVDELKVKLKGMRKQYEVGEQASPIEFVTGR
                     VKPIASIIDKANKRQIPFDRLREEMYDIAGLRMMCQFVEDIDVVVNILRQRKDFKVIE
                     ERDYIRNTKESGYRSYHVIIEYPIETLQGQKFILAEIQIRTLAMNFWATIEHTLRYKY
                     DGAYPDEIQHRLERAAEAAYLLDEEMSEIKDEIQEAQKYYTQKRSKKHEND"
     gene            complement(14160..14507)
                     /locus_tag="P6L01_000852"
     CDS             complement(14160..14507)
                     /locus_tag="P6L01_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029937.1"
                     /translation="MRLYINEIKIKDDILYCYTEDSIKGLSEVGQMLVDSDNYAFAYT
                     LDDGKAYAYLIFVQETWTMLHENTTKKIIINDELELTEFHQELTYILDNIKGNNNYGK
                     EFVATVEETFDIE"
     gene            14693..15286
                     /locus_tag="P6L01_000853"
     CDS             14693..15286
                     /locus_tag="P6L01_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDP3029938.1"
                     /translation="MATNHEIEFKQMITASIYNKLQEKYFKDSVLFKQVNYYIDTPDF
                     KLKEHRSALRIRVKDNQYEMTLKTPAKVGLLEYNYIVDIKPEMNLIISNDNLPDDIRQ
                     IIVEQFGVKDTTLSILGALTTYRQETKYKGDLLVLDKSEYFDTADYELEFEVKDYNQG
                     LQKFQSLLNELNLEHHQPLNKVQRFFKKKETLSNNIN"
     gene            15390..15755
                     /locus_tag="P6L01_000854"
     CDS             15390..15755
                     /locus_tag="P6L01_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin"
                     /protein_id="HDP3029939.1"
                     /translation="MTTTPYDIIGKEALYDMIDYFYTLVEKDERLNHLFPGDFAETSR
                     KQKQFLTQFLGGPNIYTEEHGHPMLRKRHMDFTITEFERDAWLENMQTAINRAAFPQG
                     VGDYLFERLRLTANHMVNS"
     gene            15778..16584
                     /gene="yjbH"
                     /locus_tag="P6L01_000855"
     CDS             15778..16584
                     /gene="yjbH"
                     /locus_tag="P6L01_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease adaptor protein YjbH"
                     /protein_id="HDP3029940.1"
                     /translation="MAGELRIMENKSREDINLSPVSKIEIYSFFDPFSSDCFKLSAIL
                     SKLRIEYNQYIRIRHILNPSLKVLTKCQAQSTSNFDNIALAYKAAELQGRVRAERFIH
                     LMQNEIIPKRDIITESMICDCIQNAGIDLEVFKDDLQKSKLTESLKIDLHIAREMEIE
                     QAPSLVFFSEDVHEEGLKVEGLYPYHIYTYIINELMGKPIEKNLPPKLETYIQQQQLV
                     TMEELLTIYEWPEKLLNKELKKLAIQQKIEKLKYPDGDFWKSKMPKIKSK"
     gene            complement(17044..18852)
                     /gene="pepF"
                     /locus_tag="P6L01_000856"
     CDS             complement(17044..18852)
                     /gene="pepF"
                     /locus_tag="P6L01_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDP3029941.1"
                     /translation="MSQQLSREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVENELGK
                     EEQFKGHIGDSAETLYNALELEDTLGTKLEKVYVYAHLKQDQDTTNDKYTGMESRAHQ
                     LIIKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEK
                     LLTEAQDALSTPSNVYGMFSNADLVFEDAIDKDGNAHPLTQGTFIKYLESDDRKLRES
                     AFRNVYKAYGAHNNTLGATLAGEVKKNVFNARTHNYKTAREKALSNNHIPENVYDNLV
                     KTVHKYLPLLHRFTELRKELLGLDDLKMYDLYTPLIKDIKFEMPYEEAKEWMLKALEP
                     MGEEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLTNPFILLNWSNTISDLYTLVH
                     EFGHSAHSYFSRKFQPSNSSDYTIFVAEVASTCNEALLSDYMDKHLDDEKRLLLLNQE
                     LERFRATLFRQTMFAEFEHKIHAIEEAGEPLTPTRMNEEYAKLNKLYFGDSVETDEDI
                     SKEWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGKPAVDRYINEFLKKGSSNYPI
                     EILKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA"
     gene            complement(18900..>19536)
                     /locus_tag="P6L01_000857"
     CDS             complement(18900..>19536)
                     /locus_tag="P6L01_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDP3029942.1"
                     /translation="RTEGLKKMGYKVSWLLNDVDYCHNKVKFNHFHSLFINPITRKLH
                     TFNLEKKQIMMFQQIQYLGGHKYVAEKRNAKIIELFNEAPCDYHAVYKLSKFAINQYI
                     KYCRWQNSVLEPTLSAMYQLQLTDQEVVYNYGYIFPEQIYIENHPIEWQLQVDLWLKN
                     GKSKLVNDNLNYFKLKKFIVALESKTAIIEKLINNYLNICSDRGNDVQILF"
BASE COUNT         6600 a         3415 c         2754 g         6767 t
ORIGIN      
        1 ttaaattatt aaaaccatct tgtaacatgg tataatatca taattgtttc caaaaatatg
       61 ctataattgt atactggata attttaaata tacaaataaa acatagaaca ataaattgct
      121 ttataaaata acgttttatt ttgtatcaat tcatctattg attaaaattg ctataaatgt
      181 aacgactttg cgtctttatt tcttttataa tgccgtaaaa taagttacac ttaaataaaa
      241 ttttcaaact aacggagggt ggctattaat ggttactcaa aataaaaaga tattgattat
      301 tactggctca ttcggtaacg gtcatatgca agttacacag agtatcgtta atcaacttaa
      361 tgatatgaat ctagaccatt taagcgtcat tgagcacgat ttatttatgg aagctcatcc
      421 aattttgact tctatttgta aaaaatggta tatcaatagc tttaaatatt ttagaaatat
      481 gtacaaaggg ttttattaca gccgcccaga taaactagac aaatgttttt acaaatacta
      541 tggacttaat aagttaatta atttattgat aaaagaaaag ccagatttaa tattattaac
      601 gtttcctaca ccagttatgt cggtactaac tgagcaattt aacattaata ttccagttgc
      661 tacagtgatg acagactatc gcttacataa aaactggatt acgccgtatt caacaagata
      721 ttatgtggca acaaaagaaa cgaaacaaga cttcatagac gtaggtattg atccttcaac
      781 agttaaagtg acaggtattc ctattgataa caaatttgaa acgcctatta atcaaaagca
      841 gtggttaata gacaacaact tagatccaga taagcaaact attttaatgt cagctggtgc
      901 atttggtgta tctaaaggtt ttgacacgat gattactgat atattagcga aaagtgcaaa
      961 tgcacaagta gttatgattt gtggtaagag caaagagcta aagcgttctt taatagctaa
     1021 gtttaaatct aacgagaatg tattgattct aggttatacc aaacacatga atgaatggat
     1081 ggcatcaagt caacttatga ttacgaaacc tggtggtatc acaataactg aaggtttcgc
     1141 ccgttgtatt ccaatgattt tcctaaatcc tgcacctggt caagagcttg aaaatgccct
     1201 ttactttgaa gaaaaaggtt ttggtaaaat cgctgatact ccagaagaag caataaaaat
     1261 tgtagcaagt ttaacgaacg gtaatgagca attaactaac atgattagtg caatggaaca
     1321 agataaaatt aagtatgcta cacagacaat atgtcgagac ttacttgatt taatcggtca
     1381 ttcatcacaa ccacaagaga tttatggaaa ggttccttta tatgcaagat tcttcgttaa
     1441 ataattacgc taatcataaa aatttcattt taatgcttat tatcttattt ttaatggaat
     1501 ttgcgagagg catgtatatc ctaagctata ttaatttctt gccaacagtt acatccattg
     1561 ctgtagcaat aacgtctcta gcattttcaa tacattttat tgctgatgca tctacaaact
     1621 ttgttatagg atttttactg aaaaaattcg gcacaaaaat cgttttaacg actggattta
     1681 ttttagcatt tacaagttta tttttagtta tttggtttcc agcatcacct tttgtaatca
     1741 tatttagtgc tatgatgctc ggtattgcag ttagtcctat ttgggtcatt atgttatcca
     1801 gtgttgaaga agataaacgt ggcaaacaaa tgggctatgt ttatttttca tggttgttag
     1861 gcctattggt aggtatggtt tttatgaatc tacttatcaa agtgcaccca acacgctttg
     1921 ccttcatgat gtctttagtc gttttaattg catggatatt atattatttt gtcgatgtaa
     1981 agttaacaaa ttataatact cgtccagtta aagctcaatt gagacaaatt gttgatgtga
     2041 caaagcgcca tttattgttg ttccctggta ttcttttaca aggtgccgca attgctgccc
     2101 tagttcctat attaccaaca tatgctacta aggttattaa cgtaagcaca attgaatata
     2161 ctgttgcaat cattattggt ggtatcggct gtgcagtttc gatgctattt ttatcgaaac
     2221 taatagataa ccgcagcaga aactttatgt atggtgttat tctaagcgga tttattttat
     2281 acatgatatt aatttttact ctatctatga ttgttaatat tcacatcgtg tggattatcg
     2341 ctttagctat cggtctaatg tatggcatct tattaccagc atggaatacc tttatggcac
     2401 gatttatcaa atcagatgaa caagaagaaa cttggggtgt ttttaatagt atccaaggat
     2461 tcggctcaat gatagggcca ttatttggcg gattaattac acaatttacc aacgatttaa
     2521 acaatacatt ttatttctcg gcattaatct ttttagtctt agctgtattt tatggaagct
     2581 actttatagc aaatagagaa aaagctaagt aaggttaaaa cgtattttaa aataaagaaa
     2641 atggtcattt ttattacatt caataaaaat gaccattttc ttattaattc aaacatacta
     2701 cgattaacaa tactattatg ttacttaagc taatttaaac gtttcaataa ctttccattt
     2761 atcttcgtca tcgtcaaaac gtaacaaagt taattcatcg ataatttctt tatggtctac
     2821 cccagctaat gctacttgac caaaaatatc ttcgaattct tggctagata gtccttgtgc
     2881 tattgtaaag tgtggcacaa aaacatgttc agcttctcca tagaaatctt ctccattaaa
     2941 gcgattaaac aattcttcta agtcgtccgt cttcgcaact ttaaagtaaa tcacattgtt
     3001 cgttggtttg aagctagaag cttttgtagc atgaacttct actgctggta taccattaat
     3061 acgagctcta acctgttcaa tgacagaatc taaatcacca tcttcaattt caaatggcgc
     3121 tttaattgtc acatgtggtt taattcgtga atactgttta tcatatcttt tacggtaaga
     3181 atccaccgct tcttgaaatg actttgatgg aattaatgct aatcctaaaa tcatctgaat
     3241 tcctcctttg tttaacctat tgctattata tcaaatttct ttgaaaaccg ttacattaaa
     3301 aataattcat tatacatctt ttgcaatttc gacaaattta aattaaatca aacagtaaaa
     3361 tcttttataa attatattat agaccaaact atatgaagac aattcatact aaatctacta
     3421 atgtaaatta ctcataatgt tgatcatctg tgtttttacg taaaaaatac aataatatat
     3481 ctgacagcaa tggtttccaa tctttccatt ggtgaccacc ttcaaattca tcgtaataat
     3541 aagttatatt atatttctta atttgttcag ctaattctct attcggtgtt aagaaatcgg
     3601 cacgcttacc atttgttggt aaagtaaaat ctttttcatc tagaccaatg acatgccaaa
     3661 ttgtcaattg ttctttatta gcacattgat ttagcttatc taatactttg tcatctgaat
     3721 gtggacttaa cattgctaca cgactaaaaa tcgttggata ggtcaacgcc gttaataagg
     3781 caatactacc cgctaaacta tcccctacta ataaccttgc attacctact ttcagtgtag
     3841 aaaacgtcga gtcaataaat ggcaatattt ctttaccgac tgattgaata gttaaatgag
     3901 aacgacttcc ttgtggatga aattcctctc tacgcttatc aacgtcttca taatggaatc
     3961 caacaatgat cgcatcatca atacgcgctt ctttgattaa cgattcatat gtacgttgta
     4021 ttctcccgaa acgtaaaaaa tctaatccgt caaagcaaag aatgacatta tatttaacaa
     4081 gttgattata agattctggt aaataaatac ttatcgtgac atctctattt aaaatattac
     4141 tatataaaac atgtttatta atcttaccag ttttaaattc tgacataaaa aagtgccccc
     4201 tagtagttgt attgttattg taccaagaga cactatattt ttaaaattca aatttctgag
     4261 cacctaaaat tatcaatgct gtttcttttt ttatcaaatg atcactacat atactataaa
     4321 caaaagtaga ttactttttg gcacgtgctt gttttaaacg ttctggatat gtcttcaacc
     4381 aaaagacagc attaggtaat ttattcggat caggttgata aactgcatta taaccgtttc
     4441 ctaaacgttt cttttgaatt tcataatctt ttaaagcatt tacggcaggc ttatgtaaaa
     4501 tccaaatcgc aatgatattt aaccaagcca ttagacctac acctaaatca ccgaatgccc
     4561 atgctacatc agctgtttta actgcaccgt aaaatgtagc gaacaaaata atcacacgag
     4621 caatattaat aaatatcgat gaaacttgat tattttgatt acgcgttaaa taagcaacat
     4681 ttgtttctgt aatgtagtag tacgacaaaa ttgtagtaaa tgcaaagaag aataaagcaa
     4741 atgcaataaa gtacgaaact acgccagaga aagtaggatc aaattgataa ccactgccat
     4801 ggaacgcttt atcaatgccg gcttgtgcat acatcgcagt acctgaataa tctttacctg
     4861 ttgcattttc aacataaata ccgccatctt taattaaatg cggtgtgcca ttcgcattaa
     4921 ccgtaccatc agtcacatta tatgtaccag aaataagtat aatcagagca gttgcagtac
     4981 atacaaataa tgtatcaata taaactgaaa atgcttgtac tagaccttgt ttacttggat
     5041 gtgatacttc tgctgccgct gctgcgtgtg gacctgtacc ttgaccagcc tcatttgagt
     5101 ataatccacg tttaacacca atctctatca ttgcgccaac gataccacca aaagcagatt
     5161 gtaatccaaa tgctgatttg aaaattaatg caaataacgc tggcacttct tgtatattga
     5221 tacaaatgat aatgacagcc atcagtatgt aaataattgc cataaatggt acaacggctg
     5281 ttgcaacatt ggcaatacta cgtacaccac caaaaataat taatcccaaa ataactacaa
     5341 caatagcacc cattaaccat tgtggaacat gaatcgcatt atgcatagaa cttgctatag
     5401 cgttagattg cacaccagga agcaatagac ctactgagat aatcgtaaca atagcaaaga
     5461 taattccgta aattttacca aatttaccac caataccata ttcaatataa tacgctggtc
     5521 caccacggta ttcattattt tcaactctct tgaatatttg accaagtgta gattcaataa
     5581 aagcactact tgcacctaaa aacgcagtaa tccacatcca aaatactgca ccaggtcctc
     5641 ctataaatat tgcagtagat acaccgacaa tattacctgt accaaccctg cctgctaaag
     5701 acatggctat cgcttgaaaa cttgaaatac cattaggaga cttctctcct tgaaacatta
     5761 atcgaatcat ttctttaaaa tgtctaactt gaaaaaatcg catacgcaat gtaaatagca
     5821 caccagtaat tagcaaacca taaactaatg gcttactcca aacaacctca tttaaaaagg
     5881 ttactaattt ttcaatcata agattccccc tattgtttaa acataaaaat attatacgat
     5941 taagcacgag acacttcaat atatttttca gaatattctt ataaatatta atatgatcat
     6001 ttcattattt aaacacgttt aatattaaaa taagttattc atataaactg gtgctgtttg
     6061 atccaattgc agacttacga gtcattgaaa agtctcacaa actattgaaa gtaaatatct
     6121 taaaatagaa agtgaatggt taatttaagt atatttaaaa aatattaacc tttttaagca
     6181 ctgctattta ggatatacta aataataact aagtttagaa aaataggagg aacatcgttt
     6241 atgttaaaca aggtttggtt ccgaactggc atcgctctga ttatgctgtt cattctcatc
     6301 aaactattta tggaagtgca cgaagtattt actccaatag ctacaatcat cggttctgta
     6361 tttcttccat ttttaattag tggtttttta ttttatatct gtctaccttt tcaaaactta
     6421 cttgaaaaag tcggttttcc aagatgggct agtataacaa caatcatgtt agctttgttt
     6481 gctattatcg gcttaattgt tgcgtttgtt gcaccaatca tcatttcaaa tatcaacaat
     6541 ttaattagtc agacacctgc cctacaaaaa gaagcagagc aaattattaa attcgcacta
     6601 gctcaaatgg ataaattacc tgaagatgta acaagtagaa ttacaaatat ggttaaatca
     6661 atgggtgatg gcgttactaa tattttatct aattcattac agtacattac atcattaatt
     6721 tcaaccatct tcttattgat tatggttcca ttcttcttaa tttatatgtt aaaagaccat
     6781 gaaaaattta tcccagctgt tgctaaattt ttcaaaggtg aacgtaaagt atttttcgta
     6841 gatttattaa ctgatttaaa ctttacatta aaatcttaca ttcaaggtca agtaactgtc
     6901 agtgttattt taggtatatt cttatatatc ggttattcaa ttattgactt accatatatt
     6961 cctttattag ttctatttgc tggtgtcgca aacttaattc ctttcttagg ttcatggtta
     7021 tcatttgcac ctgcagcaat actcggtatt atagatagtc ctactacatt tatatgggtt
     7081 tgtatcatta cattaattgc acaccaactt gaaggtaata tcattacgcc aaatgtaatg
     7141 ggtaaatctt taagtatcca tcctttaaca attatcgttg ttattttagc agcaggagat
     7201 ttaggtggct ttacattagt tctgattgca gtgccattat atgctgtact taaaacggtt
     7261 gttagtaata ttttcaaata ccgccaacgc attattgata aagcaaacag taatgttaag
     7321 gactaattct gtggatgtct tttaagaata taagatacta tctcctcaaa agttgaaact
     7381 acagcttttg aggagatttt tttgtgcata aaaaaaatca gtcagatgaa atatcaaata
     7441 attttccatc agtccgatta ttataaaagc aaaaaagctt tgctcacata tatgataacg
     7501 tgaacaaagc tgttgaatga tattatttaa ttgcgtggaa tccgctatct acatgaatat
     7561 tttcacctgt aacgccactt gataagtcac ttaataagta agccgctgtt ttacctactt
     7621 ctacttgatc aacgttacgt tttaaaggtg cacgctcttc gatttcttta agaattgtat
     7681 tgaaaccacc cacacctttt gcacttaatg tacggattgg accagctgaa attgcattaa
     7741 cgcgaatatt atcaggacct aagtctaatg ctaaatattt aacatttgct tctaagctcg
     7801 ctttagcaac acccatcaca ttataatttt gaactgcgaa ttcgccacct aaatatgttg
     7861 ttgcaacaat gctaccacct tctggcatta attttttagc ttcatgagcc acaattgtta
     7921 atgagtaaga actaatgtct tgagctaaca agaagccttc acgtgaagtt tcagaaaagc
     7981 gtccgcgtaa gtcttccata ttagcaaatg cgattgaatg atatacacca tcaatattgc
     8041 caacatcttt accaatttgc tcaaaaccat taataacctc ttcatcgctt tgaacatcaa
     8101 tttgatataa gtgcgcttct ggttgattta attgttctaa taatttttca agctctttac
     8161 ggctacgttc tttacggtaa gtaaatacta atttagcacc taattgatct aaaactttag
     8221 cgacaccaaa agcaatacta cgcttattag cgattcccat gatgacatat gttttgtttt
     8281 caagatttaa catgtaagat aactccttat aataattaat ttaacggata gaatatctaa
     8341 tcccagtttt aactaacttt ttattcgtca atacatttta acactattat gactttttaa
     8401 taaagttatt taaacgtatt acaacgataa atgtttgatt tagtcctttc tgtatcaaac
     8461 ttgatttacg taaaaagcat ttatttatca aaatcatata catactagca ttttaaaatc
     8521 attaatttta cgtcgcacta aaatacgtct gcattttaat atcattattg ttaaaaataa
     8581 aattctaact cttgcttcaa ttcatcaacg tattctttag caccggtaac aattaagcga
     8641 tctccatatc gcaattgtgt atctccatgc ggaacaatcg actcattatt acggataata
     8701 cgcacgaaga tgatgtctcc tccgaatggg aaattacgta attgaatatt ttcatattta
     8761 taatttaaca tttgaatttc atatagtgac gtttcaacat tacttaataa attcaacatg
     8821 ttaggtgttt caattaaacc ttttaataag attttattac ttaagtagct actgaaaatt
     8881 tcaatacctg aatcaactaa ctctgtatcg tccgttgtgc tttcaagtct gcaaatgaca
     8941 cgctcaactt gatgtgcttt ggctaattta gcaacttttc ggttaatatc atcgtcattc
     9001 gtagcacaaa caactatgtc tcggtcaaac agacctagtc gttctaaaac atcttgttca
     9061 taatcagcaa tttctatcat cgtgatatca tctgacaatt gacgacgatc actcaagtct
     9121 ttgcgataat ataatgtcac gtcatataac tgagatgtta aattttgcgc tataggaatc
     9181 gttaattgat ttttaccaat taaactaact tcaattttac ggttaaactc atctggaact
     9241 ggaaacagtt ttttgaaaat aatcggaacg aatacacatg taatgactgc gcttaaaatt
     9301 aatatacctg acgtttcagc tgaaatagca tttaatcttt ctgaaatttt ggctgcagct
     9361 atcacgagcg ataatgttga tgttaataaa aatgctgatg caatcgttgt tttcatatca
     9421 aaccaacgtc gaataaacat gactggaatt aattttgaaa tgataaatgc aacgattaaa
     9481 atcggtatga taattagtaa tttcggttct ttaattaatg aaggtatgtt taaatctaca
     9541 ccaaccatta taaagaaaat aggaataaag aaaccataac caaatgagtc taacttttca
     9601 accatttctt catctggatt taataatgaa acaacgacac ctgctaagaa tgcacctaat
     9661 atattttctg cgccaactcc ctctgctagg gctactaata atattattaa tgcaaatacc
     9721 gcacgaatac cgatttgcgt cgtaccatcc atcaattttt gtaaaaatga cattctttta
     9781 aattgaacac ctaaaatata tgaaattgct gtgaaaacaa ctaatatacc tattaaccat
     9841 attgtactgc cgccttgacc attgattgcg ccatagaccg ttaataaaat catagttact
     9901 aagtccgcaa gtactgctac taataggata aattgcccta tagttgttct cataatattc
     9961 atttctttta aagttggaac aactacgcct aaggaaatag ttgaaatgat aatgaccatt
    10021 aataacacat catccactaa tccaagccat ttaaatacat acgctaaaag aatcgaaata
    10081 atcataataa atgcaaatac agttaacgct agattaagat gccctggaat tgaggattca
    10141 tcatcatttt gtccttgacg tgcgcgttta tcttttttaa aagctttaaa atcaatttct
    10201 aaaccactta aaaacattaa aaagataaag cctaacgttg ataaaatatt tagaattgaa
    10261 tccctttcta ctatatttag aaatgaattt ccaatcacaa tccccatcaa aatttctgca
    10321 acaacaactg gcaagaaatt aatatttaat cgattgacaa ttattggagt taaaaacgct
    10381 gctaaaacaa caataactaa agataaaaac tccataactc ctccttaaat taagtaagcc
    10441 ataaatgatg tagctaaacc aaaataaatc aacatactaa taatatcatt aattgttgta
    10501 ataaatggtc cactagccac tgctggatcg atatttaatt tattcatcaa taatggaatc
    10561 atcgaaccta caaacgtccc caccgtcatc gcacaagtta aacttcctgc aacgattaat
    10621 gctaaaagtg gctgatgata tattgcaaca ataattgtaa ataatattgt tgaacataca
    10681 acgcccgata atacaccact tcccgcttct cttaatgcaa ttctaaattt actttgctca
    10741 ttaatttccc ctgtcgtaat gttacgaact gaaacggcta aagattgtgt acctgaattt
    10801 cctgacatac cactaataat aggaataaac gctgcgagta gcgctacatt ttctaatgtt
    10861 ttttcaaatc tccctaaaat tgtcgcagta atcattccta aaaatgttaa aataatcaac
    10921 catggtaaac gttttaatgc tgttttaatg attgaatcat tagtcgaatc gatatctgat
    10981 accccggcta aacgagagta gtcttcacta gcctcttcat ccataacgtc taaaatatca
    11041 tcaatcgtga tgataccaag caaatgttct tggtaatcta taacaggtac agccatgaaa
    11101 tcatagtctc tcataacttg agcaacatct tcttggtcgt ctgctacatt cacactaatg
    11161 acacgttcat tcataatatc ttcaatataa gcatcatttt cagctacaat taaatctctt
    11221 agtgataaaa cacctactaa tttaccatca tcatcaacga caaatataac atatattgtt
    11281 tctgcgtctg gcgcttgcgc tttgaccaat aataacgctt ctttaacagg cgtatgcgct
    11341 ttaagtgata aatactccgt cgtcataata ccgccggccg tatcctcatc atagtgaagt
    11401 aatgctttga tttcattcgc gtcatcttta ttcatcaatg ttaaaagact agcaactttt
    11461 ggtttagtca actcatttaa aatatctact gcattatcgt atgacatttc ttctaatatg
    11521 tgacttgcgt atgtcgcatt cattttatca aatagcaact catattcatc gtcatcaata
    11581 tctaattgat caaagaaatt tgcaacctct tcaggtgata aatattgaaa aatcttttgt
    11641 ctattttcat cggtagtatc ttcaaaatac tcactttgtt catatgtgtg taatgctaga
    11701 aattcatctc taaattgatc aatatcatca ttttctaagt attgatctaa taatgtctca
    11761 tcatataatt cctcttgaac acgctctttt tcatctgtgt tcattgacat acttacaccc
    11821 cctgtgtgaa ttagagttga ttgaataatt taagtaattg cttataatca attgtaatat
    11881 aaatattgtt cttattgatt ggatgaacaa aatatatttg cgtacattgc agtacttgac
    11941 catgaatttt gtcatgaaaa ccatcataca aagaatctcc cacaattgga tgcccaatat
    12001 gttgaaaatg tacacgaatt tgatgtgtac gtcccgtatg caaatgaact ttgcataaac
    12061 tgtatttatc attctgattt attacttcat aagatgtttt agcgtattta ccatccgagg
    12121 caacttctct agttataata ctatcctttg accgtctaat attagcttca ataataccag
    12181 atgtatgggt tttgccatat acaaggcaag tatatatttt tttcaagttt actttagaaa
    12241 ataaatgatg gatatgtcca tatttagcga atatcacaat accagttgta ttacgatcta
    12301 gacgcgtaac aatatgtggg ttaatatttt caccatgttc ctgacaatga tatagtactt
    12361 gttcgattaa actttcatga ggatgttctc tcgaaggcgt acaattttga ttgtttggtt
    12421 tagtaactat gatgataaaa gcatcttcat acaatacttc tagcttacga gcataaggta
    12481 ttaaattaac actcggtatt tctcgcggta aatgaatttc taatatatca tttggcatta
    12541 attgcttacg tactgtgact ggttcatcat taacaattaa agcgccatta tttttaatgg
    12601 cgctcactgt cttcttagaa aaatcatgtc gtgctaaaaa agctttaaca gtttcttgtt
    12661 gtgatatatg atacttaaat ttcatcgttc ttcatcactt gatatgaaag aatcgtgtac
    12721 acgtttccag aacgggaatg gtctaaaacg tgcaaacctc actttttcat ttgctacacg
    12781 gtattgtatt gaattaacat ttttatgttt gatactaaca tgatctatcg tcattctaat
    12841 ggtatcatga ttaactggtg atattaaaca tgtatgatgc tttggtaata caagtggtga
    12901 tcctaccgtt ctaaacacac gattatttat tgaggcaatt tctgtaattt gcattgcttc
    12961 aagtgaagga tgtatcagtg cgccacctag cgctttgtta taagccgttg aacccgaagg
    13021 tgttgataca cataatccat cgcctctaaa tcgctcaaag tgtttccctc ttaagttaac
    13081 atccacaaca agtgttgagc cattttcagt tttcatcgtt gcttcattta atgctaaata
    13141 ccttgtttca tagccgttgt cgttgtatct cataataatt tcaagcaatg gatattcaat
    13201 gacctgaaac tctgaattat taatttcgat gattaatttt tcaacttcat gaggtaacca
    13261 atccgcataa aatcctaaat gacctgtatg aactccaaca aatgccactt ttgataacat
    13321 gtggctatac tgatggaatg cttgtagtaa tgtaccatca ccaccaactg aaataacaat
    13381 ttcaggattt tcactatcct caatcatgcg aaaatctttc atatagttca tcattttatg
    13441 ctttaaggca ttagacttgg agtcaccttt agttaaaatt gtataacgca tgatttataa
    13501 cacctcgtta atcattttca tgttttttag aacgtttttg cgtgtaatat ttttgagctt
    13561 cctgaatttc atctttaatt tcagacatct cttcatcaag taaatacgct gcttctgccg
    13621 ctctttccaa acgatgttga atttcatccg gataagcacc atcatattta tatcgtaaag
    13681 tatgttcaat cgttgcccag aaattcattg ctaatgtacg aatctgaatc tcagccaata
    13741 taaatttttg gccttgtaat gtttcaattg gatattcaat aatgacatga tacgagcggt
    13801 aaccactttc tttagtgtta cgaatataat ctcgttcttc aattacttta aaatcttttc
    13861 tttgtcttaa aatattgacg acaacatcaa tatcttctac aaattggcac atcattctta
    13921 aaccagcgat atcgtacatt tcttctctta acctatcaaa tggtatttgt cgtttgtttg
    13981 ccttatctat aatactagcg atcggtttaa cacgaccagt aacaaattct attggcgacg
    14041 cttgttcacc aacttcatat tgtttgcgca tgcctttaag tttcactttc aactcatcaa
    14101 ccgcttgctt ataaggtgtt aagaactgat cccattgatt catagtgctt caccccgctt
    14161 tattcaatgt cgaatgtttc ttcaacggtt gcaacaaatt ccttaccata attattattc
    14221 ccttttatgt tgtctaaaat ataagtaagt tcttggtgga attcagtcaa ttctagttca
    14281 tcattgataa taattttttt agtcgtgttt tcatgcaaca tcgtccatgt ttcttgtacg
    14341 aaaatgagat aagcatacgc tttaccatca tctaatgtat acgcaaaggc ataattatca
    14401 ctatcaacga gcatttgtcc tacttcagat aatcctttaa tagaatcttc tgtataacaa
    14461 taaagtatgt catctttaat tttaatttca ttaatatata aacgcatgct taatcctcct
    14521 cttattccat aacattttaa cacaattcaa taatacaaaa cacttacgaa cactattttt
    14581 aatggtcaaa ttaacattga taatagataa aggaaattgc ttttaaaatt caactttgtc
    14641 attcataata tagttaagat aaattgaatt tgatacgagg tgtatacata tcatggcaac
    14701 aaatcatgaa atagaattta aacaaatgat aactgcatca atctataata aattgcaaga
    14761 gaaatatttt aaagacagtg tgctttttaa gcaagtcaat tactatattg atacacccga
    14821 tttcaaatta aaggaacatc gctctgcact acgcataaga gttaaagaca atcaatatga
    14881 aatgacttta aagacaccag caaaggttgg tctattagaa tacaactata tcgtcgacat
    14941 taaaccagag atgaacttga ttatttcaaa cgacaatctt ccagatgaca ttcgtcaaat
    15001 tatagttgaa cagtttggtg taaaagatac aactttatca atacttggtg cattaacgac
    15061 atatcgccaa gaaactaaat ataaaggtga tttactcgtt ttagataaaa gtgagtattt
    15121 cgatactgcg gattatgagt tagagttcga agttaaagac tataatcaag gtttacaaaa
    15181 gttccaatct ttattaaacg aactaaacct cgaacatcat caacctttaa ataaagttca
    15241 acgttttttc aagaaaaaag aaacactttc aaacaatata aattaaattg gcaaatatgc
    15301 atatttcttt caaatgttta aaatcatgtt atattagtta tatattaact taagttcgta
    15361 atagtaaatt tttgaaaatg ggtgattaaa tgacaacaac accatatgac atcattggta
    15421 aagaagcgtt atacgatatg attgattatt tttacaccct tgtagaaaaa gatgaacgac
    15481 ttaatcacct gtttccagga gattttgcag aaacaagtcg taaacaaaaa caatttttaa
    15541 cacagttttt gggcggtcca aacatttata ccgaagaaca cggacatcct atgctaagaa
    15601 aaagacatat ggattttaca atcactgaat ttgaaagaga tgcatggtta gaaaatatgc
    15661 agacagcaat taatcgcgct gcgtttccac aaggtgttgg agattattta tttgagcgct
    15721 taagattaac tgctaatcat atggtgaatt cctaaaatta attgtaggtg aataaacatg
    15781 gctggagaat tacgaataat ggaaaataag agtcgtgaag atattaatct atcacctgta
    15841 agtaaaatcg aaatttattc atttttcgat ccatttagct ccgattgctt caaattatca
    15901 gcaatcttat ccaaattaag aattgaatat aatcaatata tacgtatcag acatatatta
    15961 aatccttcgt taaaagtatt aacgaaatgc caagctcaaa gtacatccaa ctttgataac
    16021 atcgccctag cttataaagc agctgagtta caaggtcgtg tacgtgccga acgatttata
    16081 catttaatgc aaaatgaaat catacctaaa agagatatta ttacagaatc aatgatttgt
    16141 gactgtattc aaaatgcagg tattgattta gaagtattta aagacgactt acaaaaaagt
    16201 aaactaaccg aaagcttgaa aattgatttg catattgcaa gagaaatgga aatcgaacaa
    16261 gccccttctc tcgttttctt tagtgaagat gttcatgaag aaggtttaaa agtcgaagga
    16321 ttatacccat atcacatcta tacttatata attaatgaat tgatgggtaa acctatcgaa
    16381 aagaatcttc ctcctaaatt agaaacttat atacagcaac aacaacttgt aacgatggaa
    16441 gaattactta ctatttatga atggccagaa aaacttttaa acaaagagtt aaagaagtta
    16501 gccattcaac aaaaaattga aaaacttaaa tatccagatg gcgatttctg gaaatctaaa
    16561 atgcctaaaa tcaaatcaaa ataaataaat taaagacctc gtacaccata aatgtaacga
    16621 ggtcttttta ctgactctta attttgcaac attaaattat tagaacgtaa tgttacacat
    16681 cgatctaaat atattagtga tatttttcga atttaaccag ttcgaaaatt atacaaatta
    16741 taaatttggt acataataga cttatattaa tcgttaatgt ataaaaagtg catatttaat
    16801 acatttttat acaacttcac ttctctctta ttaaaaaaga acataaaaaa acgtcgtgtc
    16861 tgaatacacg acgctaaaca aaggggatgg gagaaatttt tcacttcaaa caaaggggta
    16921 tgtttgttat gtgattaatt tcatgttcat aatataacat gctatctcta ccatttcaac
    16981 cttttaatag tacttaattt aaattgtcac aatctgttca taatgtaagc gaatacaata
    17041 gtactaagct ttcattaatt tttcaaaagc gttcaatttt tgttcaaaaa cttcacaagc
    17101 ttgttcaatt ggttcaggtg ttgtcatatc tacaccagca ttttttaata tctcaattgg
    17161 ataatttgag ctaccttttt tcaagaattc attaatatat ctatctactg ctggcttacc
    17221 ttctgttaaa atttgatgac ttaagctttg agctgcactg taaccagttg cgtattgata
    17281 tacataataa ttcatataga agtgtggaat acgtgaccat tccttactaa tatcttcatc
    17341 agtttctaca gaatcaccga agtataattt atttaatttg gcatattctt cattcattct
    17401 agttggcgtt aatggttcac ctgcttcttc aattgcatga attttatgct caaattctgc
    17461 gaacattgtt tgtcggaata atgtagctct gaaacgttct aattcttggt ttaataataa
    17521 taagcgtttt tcatcatcaa gatgtttatc catataatca cttaaaagtg cttcgttaca
    17581 agttgatgca acttcagcga caaaaatagt gtagtcacta gaatttgacg gttggaattt
    17641 tctactgaag taactatgtg ctgaatgccc aaattcatga actaatgtgt ataagtctga
    17701 aatagtatta gaccagttaa gtagaataaa tggattagtt aaatgtgcac ctgatgaata
    17761 gccacctgaa cgtttacctt tattctcata gacatcgacc caacgattgt ttaagccttc
    17821 tttaactaca tttaaatatt cttcacccat tggttctaaa gcttttaaca tccactcttt
    17881 agcttcttca taaggcattt caaacttaat atctttaatt aatggtgtat ataaatcata
    17941 cattttcaag tcatctaaac ctagcaattc tttgcgcaat tcagtaaatc tatgtagcaa
    18001 tggtaaatat ttatgtacag tttttactag attgtcatat acattttctg gaatatgatt
    18061 attacttaat gctttttctc ttgcagtttt gtaattgtgt gtacgagcat taaatacatt
    18121 tttcttcact tcacctgcta gcgtagcgcc aagcgtatta ttatgagcac catatgcttt
    18181 atatacattt ctaaaagcac tttctcttag tttgcgatca tctgattcta aatacttaat
    18241 aaatgtacct tgtgttaacg ggtgtgcatt tccatcttta tcaatcgcat cttcaaatac
    18301 taaatcagcg ttgctaaaca taccgtatac atttgatggc gttgataacg cgtcctgcgc
    18361 ttctgttaat aacttttcag tttcagcatc taaaatatga ggacgttttt cattaatcaa
    18421 cttcaaatca aatgcgaatt tttgtaattt atcatatgaa tttacaaatg attgaatttt
    18481 atcttcatca atttgtaaaa tctctggcac taagaaactc catgccgagc taaatttaat
    18541 aattaattga tgtgctcttg actccatacc agtatacttg tcgttcgttg tgtcttggtc
    18601 ttgttttagg tgcgcgtata catatacttt ttctaattta gtacctaatg tatcttctaa
    18661 ttctaacgca ttgtataatg tctcagcact atcaccaatg tgtcctttaa attgttcttc
    18721 tttgcctaac tcattttcaa cttctttaaa tgcagcctca aaagcttcat catctttgaa
    18781 aattgttgtt aagtcccatg tatattcagg atatttacgt tcctgttctt ctctcgataa
    18841 ttgttgactc atatgtagta aacctcctaa taaaatataa ttgatataat tttctcattt
    18901 tagaacaaaa tttgcacgtc attacctcta tctgaacaaa tatttaaata attgttaata
    18961 agtttttcta taattgctgt tttactttct agagcaacaa taaatttttt cagtttaaaa
    19021 taattaagat tgtcatttac taatttgctt tttccattct ttaaccataa atcaacttgt
    19081 aattgccact caattggatg attttcaata taaatttgct ctggaaaaat ataaccataa
    19141 ttgtacacta cttcttgatc agttaactgt aattgataca ttgcacttaa agtgggttct
    19201 aaaacagaat tttgccagcg acaatatttg atatattgat taattgcgaa ctttgataat
    19261 ttataaacag catgataatc acaaggcgcc tcattaaaca actcaataat tttggcattc
    19321 cttttttcag cgacatattt gtgcccgcct aaatattgta tttgttgaaa catcattatt
    19381 tgttttttct ctaaattgaa cgtatgaagt tttcgagtga ttggattaat aaacaaacta
    19441 tgaaaatgat tgaacttcac tttattatga caataatcaa catcatttaa taaccaactt
    19501 actttatatc ccattttctt taaaccttcc gttcgt
//
LOCUS       DAOBBH010000033        19240 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.033, whole genome shotgun sequence.
ACCESSION   DAOBBH010000033 DAOBBH010000000
VERSION     DAOBBH010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19240
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.033"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            310..684
                     /locus_tag="P6L01_000858"
     CDS             310..684
                     /locus_tag="P6L01_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HDP3029943.1"
                     /translation="MKRILVVFLMLAIILAGCSNKGEKYQKDIDKVYKEQNQMNKIAS
                     KVQNTIKTDIKQEDSNTHVYKDGKVIVIGIQLYKDREKMYYFAYEIKDGKAEINREID
                     PIKYMKDHKADYEDENVEVEKD"
     gene            834..1232
                     /locus_tag="P6L01_000859"
     CDS             834..1232
                     /locus_tag="P6L01_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDP3029944.1"
                     /translation="MEKSIKIMTIIGIVIQGLATVFSLLLMVLAASGVMTTDVSTTVN
                     GEVDPVDAETAAAIFTVLFLSLFIFGIISIILGAIGMFKASKNKKMSGILLIIGAVIS
                     GNIITFALWLVSGIKLLTNNKPKDEISDLS"
     gene            complement(1448..2272)
                     /locus_tag="P6L01_000860"
     CDS             complement(1448..2272)
                     /locus_tag="P6L01_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDP3029945.1"
                     /translation="MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAM
                     MAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAITFSFALVLILFTNSELLTSNFMY
                     FTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
                     TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYN
                     SCLFMGGLIYQVDTLHFIPAISNIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN"
     gene            complement(2509..3816)
                     /gene="brnQ"
                     /locus_tag="P6L01_000861"
     CDS             complement(2509..3816)
                     /gene="brnQ"
                     /locus_tag="P6L01_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDP3029946.1"
                     /translation="MKKQVIISGLMLFSLFFGAGNLIFPPILGHTAGQNMWIGMLGFA
                     LTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGAFYGIPRAANVAY
                     EIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSI
                     AVIFNPESALSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRM
                     KILKYVCFSGLIAAILLGMIYFALAYVGASTAPGNFKDGTDILTYNSLRVFGSFGNIV
                     FGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIGFLFTTLGLEMILKI
                     AVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGV
                     ILKWFMMLPLADIDLAWLVPFMLFAIIGFIIDVVIRRPKQATT"
     gene            4400..5290
                     /locus_tag="P6L01_000862"
     CDS             4400..5290
                     /locus_tag="P6L01_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HDP3029947.1"
                     /translation="MNKISKYIAIASLSVAVTVSAPQTTNSTAFAKSSAEVQQTQQAS
                     IPASQKANLGNQNIMAVAWYQNSAEAKALYLQGYNSAKTQLDKEIKKNKGKHKLAIAL
                     DLDETVLDNSPYQGYASIHNKPFPEGWHEWVQAAKAKPVYGAKEFLKYADKKGVDIYY
                     ISDRDKEKDLKATQKNLKQQGIPQAKKSHILLKGKDDKSKESRRQMVQKDHKLVMLFG
                     DNLLDFTDPKEATAESREALIEKHKDDFGKKYIIFPNPMYGSWEATIYNNNYKASDKA
                     KDKLRKNAIKQFDPKTGEVK"
     gene            5539..6588
                     /locus_tag="P6L01_000863"
     CDS             5539..6588
                     /locus_tag="P6L01_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3029948.1"
                     /translation="MFLAWNEIRRNKLKFGLIIGVLTMISYLLFLLSGLANGLINMNK
                     EGIDKWQADAIVLNKDANQTVQQSVFNKKDIENKYKKQATLKQTGEIVSNGHQKDNVL
                     VFGVEKSSFLVPSLIEGHKATKDNEVLADETLKNKGFKIGDTLSLSQSDEKLHIVGFT
                     ESAKYNASPVIFTNDATIAKINPRLTGDKINAVVVRDTNWKDKKLNQELEAVSINDFI
                     ENLPGYKPQNLTLNFMISFLFVISATVIGIFLYVMTLQKTSLFGILKAQGFTNGYLAN
                     VVISQTLILALFGTAFGLLLTGVTGAFLPDAVPVKFDVLTLLVFAIVLMIVSVLGSLF
                     SILTIRKIDPLKAIG"
     gene            6601..7278
                     /locus_tag="P6L01_000864"
     CDS             6601..7278
                     /locus_tag="P6L01_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3029949.1"
                     /translation="MLKFENVTKSFKDGNRNIEAVKDTNFEINKGDIIALVGPSGSGK
                     STFLTMAGALQTPTSGHILINNQDITTMKQKALAKVRMSEIGFILQATNLVPFLTVKQ
                     QFTLLKKKNKNVMSNEDYQQLMSQLGLTSLLNKLPSEISGGQKQRVAIAKALYTNPSI
                     ILADEPTAALDTENAIEVIKILRDQAKKRKKACIIVTHDERLKAYCDRSYHMKDGVLN
                     LENETVE"
     gene            7486..8517
                     /locus_tag="P6L01_000865"
     CDS             7486..8517
                     /locus_tag="P6L01_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDP3029950.1"
                     /translation="MDLMIGTLFLFLVLVIFTLFTYKAPNGMRAMGALANAAIATFLV
                     EAFNKYVGGEVFGIKFLEELGDAAGGLGGVAAAGLTALAIGVSPVYALVIAAACGGMD
                     LLPGFFAGYMIGYVMKYTEKYVPDGVDLIGSIVILAPLARLIAVLLTPVVNSTLIRIG
                     DIIQSSTNTNPIIMGIILGGIITVVGTAPLSSMALTALLGLTGVPMAIGAMAAFSSAF
                     MNGTLFHRLKLGDRKSTIAVSIEPLSQADIVSANPIPIYITNFFGGAIAGLIIAMSGL
                     INDATGTATPIAGFLVMFGFNHPMTIVIYGVVMAIVGALAGYLGSIVFKKYPIVTKQD
                     MINRGAVDA"
     gene            8860..9978
                     /locus_tag="P6L01_000866"
     CDS             8860..9978
                     /locus_tag="P6L01_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDP3029951.1"
                     /translation="MSDSEKEILKRIKDNPFISQRELAEAIGLSRPSVANIISGLIQK
                     EYVMGKAYVLNEDYPIVCIGAANVDRKFYVHKDLVAETSNPVTSTRSIGGVARNIAEN
                     LGRLGETVAFLSASGQDSEWEMIKRLSTPFMNLDHVQQFENASTGSYTALISKEGDMT
                     YGLADMEVFDYITPEFLIKRSHLLKKAKCIIVDLNLGKEALNFLCAYTTKHQIKLVIT
                     TVSSPKMKNMPDSLHAIDWIITNKDETETYLNLKIESTDDLKIAAKRWNDLGVKNVIV
                     TNGVKELIYRSGEEEIIKSVMPSNSVKDVTGAGDSFCAAVVYSWLNGMSTEDILIAGM
                     VNAKKTIETKYTVRQNLDQQQLYHDMEDYKNGKFTKVY"
     gene            9953..10876
                     /locus_tag="P6L01_000867"
     CDS             9953..10876
                     /locus_tag="P6L01_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDP3029952.1"
                     /translation="MANLQKYIEYSREVQQARENNQPIVALESTIISHGMPYPQNVEM
                     ATTVEQIIRNNGAIPATIAIIDGKIKIGLESEDLEILATSKDVAKVSRRDLAEVVAMK
                     CVGATTVATTMICAAMAGIQFFVTGGIGGVHKGAEHTMDISADLEELSKTNVTVICAG
                     AKSILDLPKTMEYLETKGVPVIGYQTNELPAFFTRESGVKLTSSVETPERLADIHLTK
                     QQLNLEGGIVVANPIPYEHALSKAYIEAIINEAVVEAENQGIKGKDATPFLLGKIVEK
                     TNGKSLAANIKLVENNAALGAKIAVAVNKLL"
     gene            10887..12107
                     /locus_tag="P6L01_000868"
     CDS             10887..12107
                     /locus_tag="P6L01_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDP3029953.1"
                     /translation="MNILFAITGIAFALFVAFLFSFDRKNIDFKKTLIMIFIQVLIVL
                     FMMNTTIGLTILTALGSFFEGLINVSKAGINFVFGDIQNKNGFTFFLNVLLPLVFISV
                     LIGIFNYIKVLPFIIKYVGIAINKITRMGRLESYFAISTAMFGQPEVYLTIKDIIPRL
                     SRAKLYTIATSGMSAVSMAMLGSYMQMIEPKFVVTAVMLNIFSALIIASVINPYKSDD
                     SDVEIDNLTKSTETKSVNGKTGKPKKVAFFQMIGDSAMDGFKIAVVVAVMLLAFISLM
                     EAINIMFGSVGLNFKQLIGYVFAPIAFLMGIPWSEAVPAGSLMATKLITNEFVAMLDF
                     KNVLGDVSARTQGIISVYLVSFANFGTVGIIVGSIKGISDKQGEKVSSFAMRLLLGST
                     LASIISGSIIGLVL"
     gene            complement(12212..13744)
                     /locus_tag="P6L01_000869"
     CDS             complement(12212..13744)
                     /locus_tag="P6L01_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDP3029954.1"
                     /translation="MKEVGFGTLNWVAVIIYLLAMLFIGVYFTKRASQSTNSFFTASG
                     RLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFK
                     KLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVA
                     SLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVMNIKGGFGTVFADAIE
                     HKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLW
                     TNGILALISAPLFYGMGTMLYSFYTHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIA
                     AIFAAAQSTISSSLNSISACISIDIKQRFFGKDSERHEVNFARFIIIIAGIFGFGMSL
                     YLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYN
                     GVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKTAT
                     NK"
     gene            complement(13784..14665)
                     /locus_tag="P6L01_000870"
     CDS             complement(13784..14665)
                     /locus_tag="P6L01_000870"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDP3029955.1"
                     /translation="MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLY
                     VNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYD
                     ALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
                     IVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRAR
                     KIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRDIDAGLPKRPFKPF
                     NEAHRQTLDQLIAKYDL"
     gene            14823..15683
                     /locus_tag="P6L01_000871"
     CDS             14823..15683
                     /locus_tag="P6L01_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDP3029956.1"
                     /translation="MYYIAIDIGGTQIKSAVIDKQLNMFDYQQISTPDNKSELITDKV
                     YEIVTGYMKQYQLIQPVIGISSAGVVDEQKGEIGYAGPTIPNYKGTNFKRLLKSLSPY
                     VKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQGHIDNGELHKANEVGY
                     LLYRPTENTTFEQRAATSALKKRMIDGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAE
                     DVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSK
                     NDAALYGCLQ"
     gene            complement(15960..16760)
                     /locus_tag="P6L01_000872"
     CDS             complement(15960..16760)
                     /locus_tag="P6L01_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDP3029957.1"
                     /translation="MKFENRVQRYQHLFTKTDKRIVNYIRQNGYSDAFSTINSLAHAI
                     GTSPATMTRFSHKLDYENFQDLKFNIQQEMTETVIENSPIIQRIHKYHQQIIQQTGEF
                     IDNDIIQTFIDKLQSSRHILFAGLGSSGLSATEFYYRMIRMGLKGNVTTDSHLMKISA
                     SLLSHSDMFIAMSNSGNTSELISAAEVAKSHGAYVVAITNFEGSKLTDCADLVLLTTD
                     QSRNTDHQFINTQIATLFLIDIVSYHLLENTNLSQTYQHTKSIILDNK"
     gene            16900..17568
                     /locus_tag="P6L01_000873"
     CDS             16900..17568
                     /locus_tag="P6L01_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDP3029958.1"
                     /translation="MLPHGLIVSCQALADEPLHSSFIMSKMALAAYEGGAVGIRANTK
                     EDILAIKETVDLPVIGIVKRDYDHSDVFITATSKEVDELIESQCEVIALDATLQQRPK
                     ETLDELVSYIRTHAPNVEIMADIATVEEAKNAARLGFDYIGTTLHGYTSYTQGQLLYQ
                     NDFQFLKDVLQSVDAKVIAEGNVITPDMYKRVMDLGVHCSVVGGAITRPKEITKRFVQ
                     VMED"
     gene            complement(17699..19012)
                     /locus_tag="P6L01_000874"
     CDS             complement(17699..19012)
                     /locus_tag="P6L01_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HDP3029959.1"
                     /translation="MNQYHSNAQQPSAWRFFVYSLVGILCFFIPFTINGNNTIFVDHV
                     HLAIRSIIGPLMPYVALIMILIGTALPIVRRTFMTSITNLVITLFKVAGAMIGIMYVF
                     KIGPSILFKANYGPFLFEKLMMPLSILIPVGAIALSLLVGYGLLEFVGVYMEPIMRPI
                     FKTPGKSAVDAVASFVGSYSLGLLITNRVYKQGMYNKREATIIATGFSTVSATFMIIV
                     AKTLGLMPHWNLYFWITLVITFVVTAITAWLPPITNESTEYYNGQEGEQEVAIEGSRL
                     KTAYAEAMKQNALTPSLVKNVWDNFKDGLEMTVGILPSILSIGFLGLIVANYTPFIDW
                     LGYIFYPFIYIFPIADQAVLAKASAISIVEMFLPSLLVTKAAMSTKFVVGVVSVSAII
                     FFSALVPCILATEIKIPVWKLIIIWFLRVALSLLITIPVALLIFG"
BASE COUNT         6800 a         2916 c         3555 g         5969 t
ORIGIN      
        1 atttggttat gaaataaatg tgtgggaggc aatttttaga gatacatagc aatgtaaaga
       61 tatcaaatag ttattgcata atttattatg atgacataat tttcagtaga tagttgtaaa
      121 ttaacactga aatagatgtt tatattaaga agactttaat atcataatta atcaaaatct
      181 tgcgtcaaac atttaaacga attttaagtt gataacaatt ggttaaacat tgttcaaaca
      241 tcacaatgat aaagcatatt atcagtattg tagtgtgtgg aaaatgacag ccatctaagg
      301 agaaaaatga tgaaaagaat attggtagta tttttaatgt tagcaattat attggcaggt
      361 tgttctaata aaggtgaaaa gtatcaaaaa gatattgata aagtgtacaa agaacagaat
      421 caaatgaata aaattgcctc gaaagtacaa aacactatta aaacagacat taaacaagaa
      481 gacagtaata cacatgtcta taaagatggt aaagtcattg ttattggtat tcaattatat
      541 aaagatcgtg aaaaaatgta ttatttcgca tatgaaataa aagatggtaa ggcagagatt
      601 aatagagaaa tagacccaat taagtatatg aaagaccata aagcagatta tgaagatgaa
      661 aatgtagaag tggaaaaaga ttaacgtagg ctttctttaa aacttttaac cagtttcaaa
      721 aagtaaatga gctttagcga caataatttt taaaataaac acgatataaa tttaaacatt
      781 tatatataag aacacaaacg taagtttgta aataataata aaggagtaag tcaatggaaa
      841 aatcgatcaa aataatgaca ataataggaa ttgttattca gggtttagca acggtattta
      901 gtttactatt gatggtttta gcagcatcag gtgtaatgac tacagatgtg tcaacaacag
      961 ttaatggtga ggttgaccca gttgatgcag aaacagcagc agcaattttc actgtattat
     1021 tcttatccct attcatattt ggaatcattt caattatttt aggtgcaatc ggtatgttta
     1081 aagcatctaa aaacaaaaaa atgagtggta tattgttgat tattggagct gtaataagtg
     1141 gtaacataat tacatttgct ttatggttag tcagtggtat taaactactt actaataaca
     1201 agcctaaaga tgaaataagc gacttatcat aaacatcgta tattgaaatt tcaagatttc
     1261 ttaagtaatt aaataaagtg ctctcttaga agtagatttt cagtcaataa ctgcttttaa
     1321 gagagcattt tgtatttgat aatgctcata gcatcggaca aaaaccattg acgaacttaa
     1381 aaaaaatacg ttagacaatg cacttctaaa tatttaaggc atcgactaac gcattgttca
     1441 aatatagtta attattttta taaaattgat gatgatcatt caagtaagca tagaataaac
     1501 ctataatgag tccgcctcca atatagttac cgataaaagc cgcagcgata ttcgaaatag
     1561 ctggtatgaa gtgcaatgta tcaacttgat aaattaaacc acccataaat aagcaactgt
     1621 tgtaaacgac gtgttcataa cccataaagg cgaatatggt aacaccgaac atcatgacaa
     1681 acatttttgc gagtacatcg tcaatttgca tggcaataac taatgaaata ttgataaaga
     1741 aattggcgaa gatcgctttc attaatatac ttacaaaacc agtagacaac gttttgtgct
     1801 caataactgc tgataactga tttaacatat ctggcgtcat tacatttgaa aaacgcatga
     1861 aactaaataa aatagcagca cctaaaatat ttcctgcaaa gcataataaa aatattttca
     1921 atactctagt cggtttaatt actttataat acaggcctac agtaaagtac atgaagttac
     1981 tggttagtag ttcggagttt gtaaataaaa tgagtactaa cgcaaagctg aatgtaatgg
     2041 cgctagccat attcactata cctggtggta aatctggttc gtgtgttgct ttaactgata
     2101 gcacgaagac cgtaataata ccgataataa atcctgccat catcgcgcgt aataaataac
     2161 gttttaaata aacgctttgt aatatatctt ttgttcttat tgtttcgact acgttattta
     2221 cccagtcatc cccataaaaa atcttatccc atttaatatg tttctccttc acaatgacat
     2281 cctcgctttt catttgttaa tcagagtata ctatgaatcg tcttatttgt gaataatttc
     2341 acaaaattaa ttttaaaaac taatattttt gaataactga taggattttt tggtaaatca
     2401 attattttaa aataatcaat agacagacag tatcgctgaa aatccatgaa tttcatataa
     2461 aaaagcctag taacgagggg gcaatcatta ctaggcagtg agcatttatt aagttgtcgc
     2521 ttgtttcgga cgacgtataa ctacatcgat gatgaaaccg ataatagcaa agagcatgaa
     2581 tggtacaagc caagctaaat cgatatctgc taaaggtaac atcataaacc atttcaaaat
     2641 aacaccgtgt aataagttga aactatttag tatttgtaaa attgaaataa tcaatgtaat
     2701 aacagttgcg agtcgatagg cccaactgaa tctgaatgtg ctaaacatgt tagcaaatga
     2761 tatcagtaca agtgcaatag acacgggata tattaatgtc aacaatggta cagcaatttt
     2821 taaaatcatt tctaaaccaa gtgttgtaaa taagaaccct atgatagaga aaataagtgc
     2881 gaatatttta taagaaaact taggtacgtg tttcttagta aatgtggcgc aagcattgac
     2941 gagtcctata catgttgtta aacatgcaag tatcactgtc ataccaaata ctatgttgcc
     3001 aaatgaacca aatactcgta atgagttgta cgtcaatata tctgtaccat ctttaaagtt
     3061 tcctggagct gttgatgccc caacgtatgc aagtgcaaag taaatcattc caagtaatat
     3121 ggctgcaata agacctgaaa agcagacata ttttaaaatt ttcatgcgat ctgtgaggcc
     3181 tttaaactta taaccattga caatgactac ggaaaaagct aacgcagcaa caagatccat
     3241 tgtaaaatag ccttccaaac ttcctgaaat gaaaggatgt gttatatatt tatccttagg
     3301 tgcacttagt gcagattcag ggttgaaaat gacagcaata cttaatagag cgaccattaa
     3361 tagtaataat ggtgttaata atttacctaa attatcaacg attttcgatg gatttaaact
     3421 aatccagtaa acgatggcaa aaaagattgc tgcgaatata attaaagtcc attggttatg
     3481 cacaggtaaa atgtgtcttg taccaatttc gtacgcgaca tttgcagcac gtggaatacc
     3541 gtaaaatgct ccgatagaca tgtaaatcac gacagcaaaa ataaacccga accatggatg
     3601 tatacgattg cctacacttt caacaccttc atcataaaat gcaacaacaa taacagtaat
     3661 aaaggggagt aatatgcctg taagggcaaa gcctagcata ccaatccaca tattttgacc
     3721 cgctgtatgg ccaagaatgg gcgggaatat taaatttccg gctccaaaaa atagtgaaaa
     3781 taacatgagg cccgaaataa taacttgttt tttcaatgta atttccttcc taaaactaga
     3841 tattcataat aatttaaaaa atctgaaaaa taaaaacgtt cttactttat ctttaaatga
     3901 caatactatg attctattat tttataaaaa agattgcaat aataacgtct tgctattatt
     3961 ggataatgac aattaaagtg tcgtttttat gaattaaaat aggtgaaagt aagttggaaa
     4021 tacgttggag cgttgtgatt tttatgagaa attgtatgag tgaggactgc tatttataaa
     4081 aggtaaacta tggatgagtt tatggttgtt ccaataggta aataagagat agcacactgt
     4141 acacataatg atacgtggat gagtgaatat gtctgatagg aaggcctagt cactcaaatg
     4201 aatttaagtt tattacgtga taaatcacaa atctctctca tgtgataggt ctcccattaa
     4261 atcatgctta tatgaaatgt tcacatattt gttagctttt caagaaataa tattaaatat
     4321 cttttcatag tatagcaata ttaaataaat gcatttataa ttttaactgt attttaaata
     4381 ttaatcatga ggtgataaga tgaataaaat ttcaaagtat attgcaatag catcattatc
     4441 ggtagcggtt acagtttcag caccacaaac gacaaattct acagcgtttg ccaaaagttc
     4501 tgctgaagtt caacaaacgc aacaagcttc tataccagca tcacaaaagg cgaatcttgg
     4561 taatcaaaat attatggcag tggcttggta tcaaaattca gctgaagcaa aagcattata
     4621 tttacaaggt tataacagtg caaagacaca gttagataaa gagattaaaa agaataaagg
     4681 taaacataag ttagctattg ctttggattt agatgaaaca gttttagata attctccata
     4741 tcaaggctat gcatcaatac ataataaacc tttcccagaa ggttggcatg aatgggtaca
     4801 agctgctaaa gctaaacctg tctatggcgc aaaagaattc ttgaaatatg ctgacaaaaa
     4861 aggtgtcgat atctactata tttctgatag agataaagaa aaagatttaa aggcaacaca
     4921 aaagaactta aaacaacaag gtatccctca agctaagaag agtcatattt tactaaaagg
     4981 taaagatgat aagagtaaag aatcacgcag acaaatggtt caaaaggatc ataaacttgt
     5041 catgctattt ggagataatt tattagactt tacagatcca aaagaagcta cagctgaatc
     5101 tcgtgaagca ttaattgaaa aacataaaga cgatttcggt aagaaatata tcattttccc
     5161 taacccaatg tatggtagtt gggaagctac gatttacaac aataactata aagcaagtga
     5221 caaagcaaaa gataaattac gtaaaaatgc tattaagcaa ttcgatccta aaacaggcga
     5281 agttaaataa tatatgaatt ggacgtctac atgtacttaa aggtatatgt aggcgttttt
     5341 aaattgatcc ttaattttaa atgtaccgta caaaaataat agacaattaa ttgcattttc
     5401 cataaatttg cttttaatat aaaaagttcg ggtataatga aggataagtg tatgtataac
     5461 attacagagc gcaacacgac gtacgtgcat ctaagaatat aaacaatgtt taaatcatag
     5521 tagtagggag agaaatgcat gtttttagct tggaatgaaa tacggcgcaa caaattgaag
     5581 tttggactaa ttattggtgt gttaacgatg attagttact tgctattttt attatctgga
     5641 ttggcgaatg gtcttatcaa tatgaataaa gaaggcattg ataagtggca agcagatgcc
     5701 attgttctaa ataaagatgc caatcaaact gtgcaacaat ctgtttttaa caagaaagat
     5761 attgaaaata aatacaagaa gcaagctact ttgaagcaaa caggggaaat tgtgtctaat
     5821 ggccatcaaa aagacaatgt tttagtgttc ggtgttgaaa agtcatcatt tttagttccg
     5881 agtttaatag aagggcataa agcgactaaa gataatgaag tgttagctga tgaaacgctt
     5941 aaaaataaag gatttaaaat tggtgacaca ttatcactat ctcaatcaga tgaaaaattg
     6001 catatcgtag gttttacaga aagtgcaaaa tataatgcgt caccagtcat tttcacgaat
     6061 gacgctacca ttgccaagat caatcctaga ttgactggag ataaaattaa tgcagttgtt
     6121 gtacgtgata caaattggaa agacaaaaaa ttaaaccaag agcttgaagc ggtaagtatt
     6181 aatgacttta ttgaaaattt accaggttat aaaccacaga acttaacatt aaactttatg
     6241 atttcattct tatttgtcat ttcagctaca gttataggca ttttcttata tgtcatgaca
     6301 ttacaaaaaa caagtttatt tggcatatta aaggctcaag gatttacgaa tggctattta
     6361 gcgaacgtag ttatttcgca gacgctcata ttagcattat ttggtacggc atttggctta
     6421 ctgttaacag gcgttacagg tgcattttta cctgatgcag tacctgtcaa attcgatgta
     6481 ctaacattgc tcgtatttgc aattgtgtta atgattgtct ctgtattagg aagtttattc
     6541 tccattttaa caattagaaa aatagatccg ttaaaggcga ttgggtagga ggtgtagcaa
     6601 atgttgaaat ttgaaaatgt aacaaagtca tttaaagatg ggaatcgtaa cattgaagcg
     6661 gttaaagata caaattttga gataaataaa ggtgatatta tagcattggt tggaccttct
     6721 ggctctggta aaagtacatt tctaactatg gcaggtgctt tacaaacgcc gacatctggg
     6781 cacattttaa tcaataacca agatattacg acaatgaagc aaaaagcatt ggcaaaagtt
     6841 agaatgtctg aaataggttt tattttacaa gctacaaacc ttgtaccatt tttaacggta
     6901 aagcaacaat ttacattatt gaaaaagaaa aataagaatg ttatgtctaa tgaagactat
     6961 cagcaactta tgtcacaatt aggtctaact tcattgctta ataagttacc ttcagaaatt
     7021 tcaggtggtc agaaacaacg tgtggcgata gccaaagcgt tatatacgaa tccgtcgatt
     7081 attttagcgg atgaacctac cgcggcgtta gatactgaaa atgcgattga agtcattaaa
     7141 attctacgtg atcaagccaa aaaaagaaag aaagcatgta ttattgttac acatgatgaa
     7201 cgacttaaag catattgtga tcgttcatat catatgaaag atggcgtcct taatcttgaa
     7261 aatgaaacag tagaatagtt ttattaagcc ggtacatcat gtgccggtat ttttatgttt
     7321 atgtattatt tgaataaact ttcacattca attaataata attattatcg aaaattagaa
     7381 atattccgtg aaatataata ttttttgtag taaaatggcc tctaagtatt caatatttaa
     7441 atatggggat tgaatataaa attatcgtaa tgggggtcaa tggttatgga tttaatgata
     7501 ggtactttat ttttattttt ggtcttagtg atttttacat tatttacata taaagcacct
     7561 aatggtatgc gtgccatggg agcattagct aatgcagcaa tcgcaacatt tttagtggaa
     7621 gcatttaata aatatgttgg tggcgaagta ttcggtatta aatttttaga agagctagga
     7681 gacgctgcgg gaggtctagg tggtgtcgct gccgctggat taacagcatt agctatcggt
     7741 gtatcaccag tatatgcatt agttatagca gccgcgtgcg gtggtatgga tttattacca
     7801 ggtttctttg cgggttatat gattggatat gtgatgaaat atacagagaa atatgtgccg
     7861 gatggtgtcg acttaattgg atcgattgtc atcttagcgc cattagctcg tcttattgca
     7921 gtattattaa cgccagtagt gaatagtaca ttgattcgaa ttggtgatat tatccaaagt
     7981 agtacgaata cgaatccaat tatcatgggt atcattttag gtggtattat cacggttgtc
     8041 ggcacagcgc cattgagttc aatggcattg acagcattat taggtttaac gggtgtacct
     8101 atggctattg gtgccatggc agcatttagt tcggcattta tgaatgggac gctattccat
     8161 cgcttaaaat taggtgatcg taagtctacg attgcagtaa gtattgaacc tttatcacaa
     8221 gcagatattg tatcagccaa tccaattcca atctatatta caaatttctt tggtggtgcg
     8281 attgctggtt taattattgc tatgtcaggt ttaattaacg atgcgacagg tacagctaca
     8341 ccgattgcag gatttttagt aatgtttgga tttaatcatc caatgactat tgtaatttat
     8401 ggtgtagtaa tggcgattgt aggtgcgctt gcaggttatc ttggttcaat cgtatttaaa
     8461 aaatatccaa ttgttactaa gcaagacatg attaatcgag gtgcagttga cgcatagcat
     8521 catcatattg aatagtaaaa acaaataaaa catagtaacg tgattcagtc gatgaaaaaa
     8581 tcgataatga gtcacgtttt tttatagaaa aatataagac ataaaaatgt cataatttat
     8641 tgtcgacaaa tatcatactg tataaacatt tatcattttc tcaagtacct tttacacgat
     8701 ggaatgaact tactttttac gaaattatgc gtattttata aacaaatatc attgatataa
     8761 cggcaaatgt aagcgtttac aacagaaata acagcatgct acgatatttt tgtaaattca
     8821 ctgattcaag tattttaagt caatatgagg agggatgtta tgagcgattc tgagaaagaa
     8881 attttaaaaa gaattaaaga taatccgttt atttcacaac gtgaacttgc tgaggcaatt
     8941 ggattatcta gacccagcgt agcaaacatt atttcaggat taatacaaaa ggaatatgtt
     9001 atgggaaagg catatgtttt aaatgaagat tatcctattg tttgtattgg cgcagcgaat
     9061 gtagatcgta agttttatgt gcataaagat ttagttgcag aaacatcaaa tcctgtaacg
     9121 tcaacacgct ctattggtgg cgtagcaaga aatattgctg agaacttagg taggcttggc
     9181 gaaacggtcg cttttttatc tgctagtgga caagatagtg aatgggaaat gattaaacga
     9241 ttgtccacac catttatgaa tttggatcat gttcaacaat ttgaaaatgc gagtacaggt
     9301 tcatatacag ctttaattag taaagaaggc gacatgacat atggcttagc agatatggaa
     9361 gtgtttgact acattacgcc tgaattttta attaagcgtt cacacttatt gaaaaaggct
     9421 aagtgcatta ttgtcgattt gaatttaggc aaagaggcat taaacttctt atgtgcctat
     9481 accacgaaac atcaaatcaa attagttatc accacggttt cttccccaaa aatgaaaaat
     9541 atgcctgatt cattacatgc tattgattgg attatcacga ataaagatga aacagaaaca
     9601 tacttaaatt taaaaataga atctactgat gatttaaaaa tagctgctaa acgctggaat
     9661 gatttaggtg ttaaaaatgt tattgtgaca aatggcgtga aagaactcat ttatcgaagt
     9721 ggtgaggaag aaatcatcaa gtcagttatg ccatcaaata gtgtgaaaga tgttacaggt
     9781 gcaggcgatt cattctgtgc tgcagtagta tatagctggt taaatgggat gtctactgaa
     9841 gatatattaa ttgctggtat ggttaacgca aagaaaacga tagaaacgaa atatacagtt
     9901 aggcaaaacc tagatcaaca gcaactttat cacgatatgg aggattataa aaatggcaaa
     9961 tttacaaaag tatattgagt attctcgaga agttcagcaa gcacgggaga acaatcaacc
    10021 gattgtagca ttagaatcaa caattatttc gcatggtatg ccgtacccac aaaatgttga
    10081 aatggcaaca acagtagagc aaattatcag gaataatggt gccattccag caaccatagc
    10141 cattatagat ggcaaaatta aaattggttt agaaagcgaa gatttagaaa tactggcaac
    10201 tagtaaagac gttgctaaag tatctagaag ggatttagca gaagttgttg cgatgaagtg
    10261 tgttggtgct actactgtag cgacgacgat gatatgtgct gcaatggctg gtattcaatt
    10321 ttttgttaca ggaggtattg ggggcgtcca taaaggtgca gaacatacga tggacatttc
    10381 agcagactta gaagaactgt ctaaaacaaa tgtcactgtt atctgtgcag gtgccaaatc
    10441 aattttagac ttacctaaga cgatggagta tttagaaaca aaaggcgttc cagttattgg
    10501 atatcaaacg aatgaattgc cagcattctt cactcgcgaa agcggtgtta agttaacaag
    10561 ttcggttgaa acgccagaac gacttgctga cattcattta acaaaacagc agttaaatct
    10621 tgaaggtggc attgttgttg ctaatccaat tccatatgag catgccttat caaaagcata
    10681 tattgaggca atcataaatg aagctgttgt tgaagcggaa aatcaaggta ttaaaggtaa
    10741 ggacgccaca ccgttcttgt tagggaaaat tgtagaaaaa acgaatggta aaagtttagc
    10801 agcaaatata aaacttgttg aaaacaatgc ggcgttgggt gctaaaattg ctgtcgctgt
    10861 taataaatta ttgtaggtga tgatacatga atattttatt cgctatcaca gggatagcat
    10921 ttgcactatt tgttgcgttt ttattcagtt ttgatcgtaa aaacatagac ttcaaaaaga
    10981 cgttaataat gatatttatt caagtgttga ttgtgttatt tatgatgaac acaacgattg
    11041 gtttaacaat cttaactgca ttaggctcat tttttgaagg gttaataaat gttagtaaag
    11101 caggtataaa ctttgttttt ggagatatac aaaataaaaa tggctttacg ttctttttaa
    11161 atgtgttact gccattagtg ttcatttctg tattaatagg catttttaat tatattaagg
    11221 tattaccatt tattatcaaa tatgtaggta tcgctattaa taaaataacg agaatggggc
    11281 gcttagaaag ttactttgct atttcaacag caatgtttgg acaaccagaa gtatatttaa
    11341 caataaaaga tattattcca agattatcta gagcgaaatt atatacaatt gcgacgtctg
    11401 gtatgagtgc tgttagtatg gcaatgttgg gttcatatat gcagatgatt gagcccaagt
    11461 tcgtagttac agctgtaatg ttaaatattt ttagtgcgct tatcatcgct agtgtaatca
    11521 atccctataa atctgatgat agtgatgttg agattgataa tttaactaaa tcaacggaaa
    11581 cgaaatcagt gaatggaaaa acaggaaaac ctaagaaagt tgccttcttc caaatgatag
    11641 gtgatagtgc gatggatgga tttaaaatcg ctgttgtagt agccgtaatg ttgttagcat
    11701 ttatttcatt aatggaagca attaatatca tgtttggtag tgttggtttg aactttaaac
    11761 agcttattgg ctatgtgttt gcaccaatcg cattcttaat ggggattcca tggagcgaag
    11821 ctgttccagc tggctcttta atggcgacta aattaattac aaatgagttt gtagcaatgc
    11881 ttgattttaa aaatgtcctg ggtgatgtat ctgctcgaac acaaggtatc atttcagttt
    11941 acttagtaag cttcgctaat tttggtacgg ttggtatcat cgtaggttca attaaaggca
    12001 ttagtgataa acaaggagaa aaagtttcat cctttgcaat gaggctgtta cttggttcaa
    12061 ctctagcttc aatcatttca ggatcaatca ttggcttagt attgtaaatt aatcgaagta
    12121 tctaaattaa attcatggca aagctaaacc ccatcaccaa gttggcgcaa cagcgcatca
    12181 taacttggtg atgggatttt atcataacaa tctacttatt cgtagccgtt tttgaaatgt
    12241 atgttgatgg tttatctttt tcaaaaattg ttaatcccgt tatatctttt ttatgttttg
    12301 aagggacaat gaagctaagt atataagcaa agacaaaagc aactgtaaat gaaatggtag
    12361 atacatagaa aggtgagtta cctttgccaa caccattata gacataagca aagatgatac
    12421 ccaatattaa tccacaaata acaccgaatg tattcgtacg tttagtgaaa ataccaactg
    12481 caaatacacc agctaatgga acaccgaata atccagttac aaacaagaat aaatcccata
    12541 agtcatttga attagaagca attaagtata gtgacattcc aaaaccgaaa atacctgcaa
    12601 tgatgataat gaaacgtgca aagttaactt cgtgtcgctc gctatctttt ccgaagaagc
    12661 gttgcttaat gtcgattgaa atacaagcag atatagaatt taaactagat gaaatggtag
    12721 actgtgcagc ggcgaaaatg gctgcaataa gtaatcctgc tacaaatggt ggcatctcag
    12781 tcaaaatgaa atatggcact acagatgatg tattgaagcc ttttggtaaa acagcttcat
    12841 gtgtataaaa tgaatacagc attgtaccca taccataaaa taagggtgct gaaattaaag
    12901 ctaggatacc atttgtccat aacgatttat ttgtttcttt taaactatca gaagcttgat
    12961 aacgctgcac gacgtcttga ctcgctgtgt attgatacaa gttgttgaaa atatttccta
    13021 ggaaaataat tggaatggca gctgccgcag tatttagttt ccaattgtct gcactaatta
    13081 attttttgtg ctcaatcgca tctgcaaaga cagtgccaaa accgccttta atgttcataa
    13141 cacctagaat aataataact aaagcgccgc ctaataaaat gacgccttga atgaaatcac
    13201 tccaaaccac accttcgaaa ccacctaaaa atgtatataa aatacatagt aaacctacga
    13261 gtgatgcaac gatataagga ttcatgtctg atacagatgt gattgctaat gttggtaagt
    13321 agataacaat tgcaacacgc cctaaatggt aaacgacaaa taataatgag ccaatgacac
    13381 gtatgctagg gccaaatcta gcttctaaat attcatatgc agatgttacc tttaactttt
    13441 taaagaaagg gacatagaaa taaataagta atggaataat tgcgacgata gcaatgttac
    13501 cagcgatata tgaccaatct gttaaaaatg ctttctctgg tgtagacata aatgtaatcg
    13561 cacttaacgt agtagcataa attgaaaagc caactaccca agatggcaag cgaccacttg
    13621 cggtaaagaa actattggta ctttggctcg cgcgcttggt aaaataaacg ccaatgaaca
    13681 acatagctag tagataaatg ataacggcaa cccagtttag tgtgccaaat ccaacttctt
    13741 tcatgggcaa catccccttt acaatgtatt gattctttga tgtctataaa tcgtattttg
    13801 caatgagttg atctaatgtt tgtcgatgtg cttcgttaaa aggtttgaaa ggtcttttcg
    13861 gtaatcctgc atcaatgtca cgatgacgta atatttcttt caatgttgga taaatcccca
    13921 ttgataacac tgtttcgata atgtcgtttg aatcatgttg tagttggtaa gcttcttgaa
    13981 tttgaccttg tcgtgctaag tcgaagattt ttctagcgcg gcgaccatta acgttatatg
    14041 tagaaccaat tgcaccatct acgccagaaa tcgtagcttg aactaacatt tcatcaaagc
    14101 cagataagat taatttgtct gggaatgctt ttctaatacg ttcgagtagg aagaagttcg
    14161 gcgctgtata tttaacacca acaatttttt cgtgattaaa tagctcgctg aattgttcaa
    14221 tagaaatatt cacacctgtt aaatctggta ttgcataaat aatcatattg ttctgtgttg
    14281 cttcgataat atcgaaatag taatctctaa tttcttcaaa agtaaatgga tagtagaatg
    14341 gtgttacggc agaaagtgca tcataaccga gttctgtggc atattttccg agttcaatgg
    14401 cttcatttaa atctaacgaa cctacttgag caatcaattt cactttatcc ccaactgcct
    14461 ctttggcaac cttgaaaact tgcttcttct gctctgtatt taataaaaag ttttcgcctg
    14521 agctaccatt tacataaaga ccgtctaatt cttcagtttc aatggcattt tgagcaattt
    14581 gtttaagtcc ttgttcattt acttgaccat tttcatcaaa aggaacgagt aacgctgcat
    14641 ataaaccttt taaatctttg ttcattatga agtccctcca aaaatcattt gataatatag
    14701 tttacagcta taattgtaaa cgctatcata aaatgtaaca atatcttttt gaaaattgta
    14761 gtcatattta tgtataatta atgaaaatgt ttttcaaaat caatagaaat ggagtgagta
    14821 aggtgtatta catcgcaatc gatattggag gcactcaaat taaatcggca gttattgata
    14881 agcaattgaa tatgtttgac tatcaacaaa tatcaacgcc ggacaacaaa agtgagctta
    14941 ttactgacaa agtatatgag attgtaacag gatatatgaa gcaatatcag ttgatccaac
    15001 ctgtcatagg tatttcatca gcaggcgttg ttgatgaaca aaaaggcgaa attggatacg
    15061 cagggccaac cattccgaat tataaaggta ctaattttaa gcgattatta aaatcactgt
    15121 ctccttatgt caaagtaaaa aatgatgtaa acgctgcatt actaggcgaa ttgaaattac
    15181 atcaatatca agcagaacgg atcttttgta tgacgcttgg tacaggcatt gggggtgcgt
    15241 acaagaataa tcaaggtcat attgataatg gtgagcttca taaggcaaat gaagttgggt
    15301 atttattgta tcgtccaact gaaaatacaa cgtttgagca acgtgctgca acgagtgcat
    15361 tgaaaaagcg catgattgac ggaggattta cgagaagcac acatgtgcca gtattgtttg
    15421 aagcagctga agaaggtgat gatattgcaa aacaaatatt gaatgagtgg gcagaagatg
    15481 tagcagaagg gattgcccaa atacaagtca tgtatgatcc agggcttata ttaattggtg
    15541 gcggtatatc tgaacaagga gacaatctca ttaaatatat cgagccgaaa gttgcacact
    15601 atttaccaaa agactatgtt tatgcaccaa tacaaacgac taagagtaaa aatgatgcag
    15661 cattatatgg ctgtttgcaa tgatagttga aagaaggagt cattctaaaa tagaatttga
    15721 aaccgttacg agagatgaga gctgttgtta gttccacaca tcacactcta tctaggacca
    15781 atctaaacta tatcaaccaa cagtgtgcca cgggcatatt aaattgaaga cggttgaacg
    15841 tatagatctc aaaaatgtaa aaaagtattt ggtattgaaa ttaaaaaagc acctagcaac
    15901 tcgttaggac aatcacgatg attgtctaca gttgcaggtg gatttgaata tactactagt
    15961 tatttgttgt ctaggataat agatttagta tgttgataag tttgactcag atttgtattt
    16021 tctaataaat gataactcac gatatcgatt aaaaagagtg tcgcaatttg tgtgttgata
    16081 aattgatggt cggtattacg cgattgatcc gttgttaaaa gtactaaatc tgcacaatct
    16141 gtaagtttac taccttcgaa atttgtgatg gcaacgacat atgcaccatg agatttggcg
    16201 acttccgctg ctgaaattaa ttccgaagta ttaccactat ttgacatagc aataaacatg
    16261 tccgaatgag atagtaggga tgccgatatt ttcattaaat gtgaatcggt agtaacatta
    16321 ccttttagcc ccatacgaat catacgataa taaaattcag tcgctgataa accagagcta
    16381 cctagtccag caaagagtat atgtcgactt gattggagtt tgtcgataaa ggtttggata
    16441 atgtcgttat caataaattc accagtttgt tgaatgattt gttgatgata tttatgaatt
    16501 ctttgaataa ttgggctatt ttcaataact gtctctgtca tttcttgttg aatattaaat
    16561 tttaaatctt ggaaattctc ataatctagc ttatgactaa agcgtgtcat cgttgctggt
    16621 gatgtaccaa tcgcatgggc taaggagtta atcgttgaaa aggcatcgct ataaccattt
    16681 tgtcttatat aattgacgat gcgtttatca gtttttgtaa ataaatgttg ataacgttga
    16741 acacgattct caaatttcat tgtgtcaccc cttcatctta atgattacta ttatatatga
    16801 aaaatatttt caagatagta aaaagcattg ataaaaatta tcttaatgat atattgtaaa
    16861 tgactttacg tgaaaaaacg acttatggag tgaggaataa tgttaccaca tggattaata
    16921 gtatcttgtc aggcactagc agatgaacca ttgcattcat cttttattat gtcgaaaatg
    16981 gcattagctg cgtatgaagg tggtgctgtt ggtattcgcg caaatactaa ggaagacatt
    17041 ttagcaatta aagaaacggt agatttacca gttattggca ttgtgaaacg tgactatgat
    17101 cactcagatg ttttcattac tgcaacgtca aaagaagttg atgaactgat agaaagccaa
    17161 tgtgaagtca ttgcattgga tgcaacgtta cagcaacgtc cgaaagaaac gttagacgaa
    17221 ttagtatcat atattagaac acatgcaccg aacgttgaaa tcatggctga tatcgcgacc
    17281 gttgaagaag ctaaaaatgc cgcacgactt ggctttgatt atattggcac gacgttacat
    17341 ggctatacta gttatacgca aggacaatta ctttatcaaa atgacttcca atttctaaaa
    17401 gatgtactac aaagtgttga tgcaaaagtt attgcggaag gtaatgtcat tacaccggat
    17461 atgtataaac gcgtgatgga cttaggcgtt cattgttcag tcgttggtgg tgcgataaca
    17521 cgaccaaaag aaattacgaa acgttttgtt caagttatgg aagattaaat gataacgata
    17581 aaaaaacgag atgaccatca ttaattaaag gtacctaatt atcttaggtg gctgaatgaa
    17641 tgtaatgggt tcatctcgtt ttgtttgttt acgatagtga ttttattttc aatttgaatt
    17701 atccaaaaat aagtaaagcg acggggatgg tgattaatag cgacaatgcc acgcgtaaaa
    17761 accaaatgat gatgagtttc cagacaggta ttttaatttc agttgctagt atacatggca
    17821 ctaatgctga gaaaaagata atggctgata cgcttactac accgacgaca aatttagtac
    17881 tcattgcagc tttagttact aacaaagatg gtagaaacat ttctacaata gaaatcgctg
    17941 acgcttttgc aagtacagcc tgatcagcaa ttgggaaaat ataaataaat ggatagaata
    18001 tatagccaag ccaatcaatg aatggtgtat agttcgctac aatcagtcct aaaaaaccaa
    18061 tcgataatat agaaggcaaa ataccaacag tcatttctaa accgtcttta aaattgtccc
    18121 aaacgttctt cacgagagat ggtgttaatg cattttgttt catcgcctct gcatatgcag
    18181 ttttcagtct gcttccttca atagcaactt cttgttctcc ttcttgtccg ttataatatt
    18241 ctgttgattc attggtgatt ggcggtagcc atgcagtaat tgcagtcacg acaaatgtga
    18301 tgactaaagt tatccaaaag tataaattcc aatgcggcat tagccctaaa gttttagcaa
    18361 cgataatcat aaaagttgct gaaactgttg aaaagccagt cgcaataatc gtggcttctc
    18421 gtttgttgta catcccttgc ttatagacac gattagtaat caataatcct aaggaataac
    18481 tgccgacaaa cgaagctact gcatcgacag cggattttcc tggtgtttta aaaataggtc
    18541 tcataatagg ctccatataa acaccgacaa attctaataa gccatagcct actaataaag
    18601 aaagcgcaat tgcacctact ggaattaaga tacttaatgg catcattaat ttttcaaaca
    18661 agaacggacc atagttagct ttaaatagta ttgatggacc gattttaaat acatacatta
    18721 taccaatcat tgcacctgca actttaaata atgtaatgac caagtttgtg attgaagtca
    18781 taaaagtacg tctcactatt ggtaacgctg taccaattaa aatcataatc agtgcaacat
    18841 agggcataag tggacctatg attgagcgaa tggctagatg aacatgatcg acgaaaatag
    18901 tgttgttacc attaatcgta aaaggaataa agaaacatag tatgcccact aaactataga
    18961 caaaaaaacg ccatgcactt ggttgttgtg cattagaatg atattgattc attaaagcaa
    19021 cccctttgtt taaatgaata cacaaaactg tatgatgcat cttcccctta atgagatgaa
    19081 tcattatttt aatttagaaa aatctgaaaa cttactataa ttgtatagtt tgaattattt
    19141 tcataccaat acaaattaac taattatata tagattgaaa ctatattact taataaaata
    19201 tttatcttaa atgttgttgt gttgattcaa cgccacaact
//
LOCUS       DAOBBH010000034        19110 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.034, whole genome shotgun sequence.
ACCESSION   DAOBBH010000034 DAOBBH010000000
VERSION     DAOBBH010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19110
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.034"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            146..877
                     /locus_tag="P6L01_000875"
     CDS             146..877
                     /locus_tag="P6L01_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HDP3029960.1"
                     /translation="MNGNIIVAHGMRHGRQNQALEAFISELVKDDIHHYDIAFLESEH
                     QDLETVMTTLIQSGVDHFKIVPLLIFSAMHYLKDIPNIVHEMKRRYPDIKVEVSEPLG
                     THPLMRRLIEQRICDALAGDEQQVGVMVVAHGNINGNFTKAHEELQICAQHLQINKPT
                     YARTLYGEISFTHDLESISKRYQKLIVVPLFLYDGRLVNKVKQQMNDMVINTDIHFTP
                     SINFDPILKQIINDRLESLMIPMKI"
     gene            943..3348
                     /locus_tag="P6L01_000876"
     CDS             943..3348
                     /locus_tag="P6L01_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDP3029961.1"
                     /translation="MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYN
                     RIMLSNILQNKMTVEETIMNPYEWYEEHGIELITNDPVIEVDRANQSVTTANGIEVSY
                     DKLIFATGSKAFVIPVPGSTLPSVIGWRTIDDTEQMMNIAKTKKKAIVIGGGLLGLEC
                     ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDD
                     VEGVKLADGREIPADLVVMAVGIRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVG
                     ECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIV
                     ENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTL
                     VSLLTKGGEEASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGN
                     SCGKCKPQIAQILQHTLGDDFVAAKPAGICGCTDLTRDQIVTQIRAKGLKTSKEVRHV
                     LNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSVIPQMR
                     GGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYG
                     KKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGV
                     KDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEVIKLCGALMQYYRETGIYAE
                     RTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARKIFEV
                     EKV"
     gene            3352..3666
                     /gene="nirD"
                     /locus_tag="P6L01_000877"
     CDS             3352..3666
                     /gene="nirD"
                     /locus_tag="P6L01_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HDP3029962.1"
                     /translation="METKEKIKVTTIDELTPLIGKKVIVKGKEVGLFLTESGTIHAIH
                     NICPHKQGPLSEGTVSGEYVFCPLHDQKIDLNTGIVQEPDEGCVDVYEVEVTDGNVYI
                     CL"
     gene            3657..4634
                     /gene="cobA"
                     /locus_tag="P6L01_000878"
     CDS             3657..4634
                     /gene="cobA"
                     /locus_tag="P6L01_000878"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDP3029963.1"
                     /translation="MSVDKYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNP
                     LILQYANLTTEIIDVGKKPYAKHIQQEKINECIVEAARRYNKVVRLKGGDPAIFGRVQ
                     EEVDTLNNFNIAFEIVPGVTSASAAVATMQTGLTMRAVAKSVTFSTGHFKDSEENEVD
                     VNALVNGGTLAIYMGVKRLGKIIAQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNIV
                     GKLEHYAIEAKPGICIIGEVVGYTENVSTTSNPTQQFYVVSGSRHDALMLCEHLYDEG
                     YGCLLNPNDTSNGTYHSSQYDYYDAFIKQQENVTYISTDRADANTVLCH"
     gene            4943..8632
                     /locus_tag="P6L01_000879"
     CDS             4943..8632
                     /locus_tag="P6L01_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDP3029964.1"
                     /translation="MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVR
                     TTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYS
                     PLRIKYPYIRGKLWDLWTEALEENYGNRVAAWASIVENEDKAKQYKQARGMGGHVRSN
                     WKDVTEIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDW
                     YADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKV
                     ISVAPDYAENVKFADNWLAPNPGSDAAIAQAMTHVILQEHYVNQPNERFINYAKQYTD
                     MPFLIMLDEDENGYKAGRFLRASDLGQTTEQGEWKPVIHDAISDSLVVPNGTMGQRWE
                     EGKKWNLKLETEDGSKINPTLSMAEGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLA
                     NGDKVRIATIFDLMASQYGVRRFDHKLESKGYDDAESKYTPAWQEAISGVKQSVVIQV
                     AKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVG
                     QEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTED
                     LKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIESNEAILKRAINEVKSK
                     QTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNEDEKP
                     EEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFN
                     PAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVV
                     KDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQY
                     EELKSMLGTWSDTNDDSVRANRPRIDTARNVADAILSISSATNGKLSQKSYEDLEEQT
                     GMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNIERLVPFR
                     TLTGRQSYYVDHEVFQQFGESLPVYKPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGK
                     WNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISH
                     RMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNY
                     YGPIGNQRDLYVAVRKMKEVNWLED"
     gene            8622..10181
                     /gene="narH"
                     /locus_tag="P6L01_000880"
     CDS             8622..10181
                     /gene="narH"
                     /locus_tag="P6L01_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDP3029965.1"
                     /translation="MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNV
                     ETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDY
                     YEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
                     IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACR
                     GWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYD
                     ADRVHEAASAVDEKGLYEKQLDIFLNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLA
                     IEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYL
                     ANIFTAGDTEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLG
                     LAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIY
                     NENFYGGIFRD"
     gene            10174..10749
                     /gene="narJ"
                     /locus_tag="P6L01_000881"
     CDS             10174..10749
                     /gene="narJ"
                     /locus_tag="P6L01_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDP3029966.1"
                     /translation="MINFDNFKKYQESFGYMAQQLCFPEKLTFHPKTFEETISKDHPA
                     YEDLVAFRNVMMTFSLSEIKAIYTDTFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVL
                     YEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALG
                     EDNNPYAYVIQALRKTLKLCLTSPKGVKHHA"
     gene            10742..11419
                     /gene="narI"
                     /locus_tag="P6L01_000882"
     CDS             10742..11419
                     /gene="narI"
                     /locus_tag="P6L01_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDP3029967.1"
                     /translation="MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERK
                     RLMWGSLLFHLGIIPVFFGHVVGLLIPKSWMDAVGVSEHLYHIGAVYIGSIFGIITLI
                     GMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLS
                     IWFRQLFMLKPDASLMSGVPIAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYI
                     IYRKNKV"
     gene            11439..11891
                     /gene="nreA"
                     /locus_tag="P6L01_000883"
     CDS             11439..11891
                     /gene="nreA"
                     /locus_tag="P6L01_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation accessory nitrate
                     sensor NreA"
                     /protein_id="HDP3029968.1"
                     /translation="MTPEAIIEDRRFQETLDKIRKEEGYDFAAIAFYESNKPSSPIKW
                     HYVSGNKNNRFKLIILRKGRGLAGTVMKTGKRMVIANVGLALGPEEKIDYPILLSESL
                     TAVLAVPLWYKNQVYGVLLFGQRDGRPLPKIFDNDDIQRKFGIFNDDK"
     gene            11915..12949
                     /gene="nreB"
                     /locus_tag="P6L01_000884"
     CDS             11915..12949
                     /gene="nreB"
                     /locus_tag="P6L01_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501138.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation sensor histidine
                     kinase NreB"
                     /protein_id="HDP3029969.1"
                     /translation="MINEDSIQLDTLLKKYYEHSIEKIVFADDNGKIIAMNDAAKDIL
                     SEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPF
                     TATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDS
                     VIQEMLNVDVQLRLLKYQEDTTKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLG
                     LEAAFKSYFKQFEENYGIKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNE
                     INVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGE
                     GTNVTLNIPI"
     gene            12972..13625
                     /gene="nreC"
                     /locus_tag="P6L01_000885"
     CDS             12972..13625
                     /gene="nreC"
                     /locus_tag="P6L01_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464689.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation response
                     regulator NreC"
                     /protein_id="HDP3029970.1"
                     /translation="MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVME
                     YKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYI
                     LKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKREL
                     EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF"
     gene            complement(13883..14623)
                     /locus_tag="P6L01_000886"
     CDS             complement(13883..14623)
                     /locus_tag="P6L01_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDP3029971.1"
                     /translation="MAYTYTLKDIIEITGVTKRTLHYYDEIGLLVPDKNDKNYRVYKQ
                     QDLEKLQKILILKSFDFDIAKIKQYISYDNEQLRKLLSEQISKLDKKISDLQLIRRSV
                     CEFIKGLSLIDTSILNKTLQSQYDKEASIKYGHTKAYQSFIRRKDSLQSQDIRHKLTT
                     IFNKFNHMSLSHYPIQDCSDLVFEWKAFMNTIADFDDETLCCIAKTYEDDTRFKDYFN
                     SYDNQNLASYISEAVNYFLSNVNKSDNF"
     gene            complement(14602..15516)
                     /locus_tag="P6L01_000887"
     CDS             complement(14602..15516)
                     /locus_tag="P6L01_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HDP3029972.1"
                     /translation="MYKINIIRSDSVYKNILKAPINDRDSIFTKEILVPFKKKFEVQH
                     MPIYNDDKQTMSAIQFLDAFQISPKDLRMSDQMSIQYLNNDFWSNCEKYLKVAIDQFS
                     NYSISSQVSNYHFTVLLGDSQKPLMYLNKNRGGDGGIPGYIMIYLVPSTSTINSMKSL
                     IAHEVNHNMRYQYIDWDGGSLIELIIAEGLAENYVESLYGKAHIGPWVTNTNWSRDNV
                     KIKNTIYNHLHLKHIFESMPYLYGDDINKLQGRPIVGLSHAAGYACGYHLVKYFLQKT
                     NIPIEVATTLPAQKIINEVTEFWHTHTL"
     gene            15885..17054
                     /locus_tag="P6L01_000888"
     CDS             15885..17054
                     /locus_tag="P6L01_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK/NasA family nitrate transporter"
                     /protein_id="HDP3029973.1"
                     /translation="MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQ
                     ISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMAS
                     GFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
                     TTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGA
                     FVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDF
                     VIMIIGAVILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFSNEAGSANGIVSM
                     MGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKHS"
     gene            complement(17161..17589)
                     /locus_tag="P6L01_000889"
     CDS             complement(17161..17589)
                     /locus_tag="P6L01_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDP3029974.1"
                     /translation="MILNFNQFENQNFFNGNPSDTFKDLGKQVFNYFSTPSFVTNIYE
                     TDELYYLEAELAGVNKEDISIDFNNNTLTIQATRSAKYKSEQLILDERNFESLMRQFD
                     FEAVDKQHITASFENGLLTITLPKIKPSNETTSSTSIPIS"
     gene            complement(17805..18251)
                     /locus_tag="P6L01_000890"
     CDS             complement(17805..18251)
                     /locus_tag="P6L01_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDP3029975.1"
                     /translation="MYVENSYLSKQLCFLFYVSSKEIIKKYTNYLKEYDLTYTGYIVL
                     MAIENDEKLNIKKLGERVFLDSGTLTPLLKKLEKKDYVVRTREEKDERNLQISLTEQG
                     KAIKSPLAEISVKVFNEFNISEREASDIINNLRNFVSKNFDYSDRK"
     gene            18648..19013
                     /locus_tag="P6L01_000891"
     CDS             18648..19013
                     /locus_tag="P6L01_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDP3029976.1"
                     /translation="MKKKKNAIKVIIILFISLIVAVAFFFGLKTYQGHKNIQLIDSYL
                     EEKNLKDKIKSEKTEYSAKKGLFYKEVTFKDEPGVTYVVQPISTNKGLFVEGFDTETK
                     KSLKTAKHKYFNQNYKPSK"
BASE COUNT         6623 a         2758 c         3768 g         5961 t
ORIGIN      
        1 ttttgattta acaaaaaggg aaaatgtata aggaatatgc atgactacag gcatcaggga
       61 attccctgat tttacattat aatgtgattc ttttcacaaa aacaaaatga tttggtatac
      121 aatacgtgtg aaagggttgg taaatgtgaa tgggaatatc attgttgcac atggcatgag
      181 gcacggacga cagaatcaag cgttagaagc atttatttct gaattagtaa aagatgatat
      241 acatcactat gatattgcgt ttttagaaag tgagcatcag gatttagaga cggtaatgac
      301 gacgttgatt caaagtgggg ttgaccattt taaaattgta ccattgctaa tctttagtgc
      361 aatgcattat ctcaaggata taccgaatat cgttcatgaa atgaaacgac ggtatccaga
      421 cataaaggta gaagtaagcg agccacttgg cacacatcct ttaatgagac gattaattga
      481 acaacgtatt tgtgacgcat tagcaggtga cgaacaacaa gttggagtta tggttgttgc
      541 acacggcaat attaatggaa attttactaa ggcacacgaa gagttacaaa tatgcgctca
      601 acatttgcag attaataaac ctacttatgc gagaacgcta tatggagaga ttagttttac
      661 acatgattta gaaagcatct cgaaacggta tcaaaaattg attgtcgtac cattattttt
      721 atatgacgga agacttgtta acaaagtgaa gcagcaaatg aatgacatgg ttattaatac
      781 agatatacac ttcacacctt caattaactt cgacccaatt ttaaagcaaa tcattaacga
      841 tagattagaa agcttaatga ttccgatgaa aatataaaag cactaacgaa aaattaatac
      901 ctttttttga aataaatatt atggagtaaa ggggcatatt ttatggcaaa gcaaaaacta
      961 gtaatgattg gtaacggtat ggcgggcatt cgaacaatcg aagaaatatt agagcgcgcc
     1021 aacgatttat atgatattac ggtaataggt aaagaacctt atccaaacta taaccgcatc
     1081 atgctttcaa atattttaca aaataaaatg acagttgaag aaacaattat gaatccatat
     1141 gagtggtatg aggaacatgg tatcgagtta attaccaatg atcctgtaat tgaagtagat
     1201 agagcaaatc aatcagttac tactgcaaat ggtattgaag tatcatacga taaattgatt
     1261 tttgcgacag gttctaaagc attcgtcatt ccagttccag gttcgacatt accgagtgta
     1321 attggttgga gaacgattga tgatactgaa caaatgatga acattgcgaa gactaaaaag
     1381 aaagcaattg taattggtgg aggcttacta ggtttagagt gtgcacgtgg tttattagat
     1441 caaggtatgg aagtaacggt gttacattta gctgaatggt tgatggaaat gcagctagac
     1501 cgtaaagcag gaaatatgct taaagcagat ttagaaaagc aaggtatgaa gtttgaaatg
     1561 caagccaaca caactgaaat cttaggagaa gatgatgttg aaggtgttaa attggctgat
     1621 ggacgcgaga ttccggcaga cttagttgtt atggcagtag gtatacgacc atacacagaa
     1681 gtagccaaag aatcaggtct agatgttaat cgtggtattg ttgtcaatga tgtgatgcaa
     1741 acaagtgata gcaatgtata tgcagttgga gaatgtgcag aacataacgg caaagtttat
     1801 ggactcgttg caccactata tgaacaaggt aaagtattag ctgatcattt aacaaataaa
     1861 gaaacgaacg gatacaaggg atcaacaaca tttacgtcat taaaagtttc tggttgtgac
     1921 ttgtatagtg ctggtcaaat tgtagaaaat gcagaaatta aaggtattga aatatttaat
     1981 agtgttgata ataactataa aaaaatcttt ttaaaagacg gtaatgtagt tggtgcagta
     2041 ctatatggtg atatcgatga tggttcacgc ttttataaca tgatgaaaaa aggtgaatcc
     2101 actgaagatt acacacttgt atcattgctt actaaaggtg gagaagaggc atcgctatca
     2161 attgctgata tggctgatga tgaaacaatt tgtggttgta atggtgttga taaaggtact
     2221 atagtaaatg cgattacgga aaatggcttt acaacagttg aagaagtaac ggctaaaaca
     2281 aaagcgggga attcatgtgg taaatgtaaa ccgcaaattg ctcaaatatt gcagcacacc
     2341 ttaggagatg actttgttgc cgcaaaacct gctggtatat gtggttgtac tgatttgaca
     2401 cgcgatcaaa ttgtaacgca aataagagcg aaaggtttaa aaacatctaa agaagttcga
     2461 catgttttaa actttaaaaa taaaggtgga tgtccaaaat gtcgaccagc aatcaactat
     2521 tatttaaaca tggtttatcc acatgatcat gaagatgaaa gagaatcaag atttgctaac
     2581 gaacgttacc atgcgaatat tcaaaatgat ggtacatttt ctgttatacc tcaaatgcgt
     2641 ggaggtgtta cagatgcaga ccaactgatt cgtctaggag aagtggctaa gaaatatcat
     2701 gtgccactag ttaaagtgac aggttcgcaa cgtgttggtt tgtatggagt taaaaaagaa
     2761 gaattaccaa atatatggga agacctaggt atgcgttcag catcagctta tggtaagaaa
     2821 acacgctcag ttaaaagctg tgttggtaaa gagttttgtc gatttggtac gcaatacacg
     2881 acacgacttg gcattcgttt agaaaaaaca tttgaataca tcgatacacc tcataaattc
     2941 aaaatgggtg tatcaggctg cccaagaagt tgtgttgaat caggtgttaa agattttggt
     3001 attatctcag ttgaaaatgg attccaaatc tatatcggtg gtaatggtgg tacagaagtc
     3061 gaaaaagctg aatttttaac aactgtagaa acagaagatg aagtaatcaa attatgtggt
     3121 gctttgatgc aatattatcg cgaaacaggt atatatgctg aaagaacagc gccatggtta
     3181 agaagacttg gatttgaaaa tgtaaaagaa gtcttacttg acccagaaag acaaaatgaa
     3241 ctatttgaac gcattatgga tgctaaaaaa gcagtcgaag ctgaaccttg ggaagcaata
     3301 actagcaatg cgcaagcacg taaaattttt gaagtggaga aggtgtaata catggaaaca
     3361 aaagaaaaaa ttaaagtgac aactatagat gaattaacac ccctaattgg aaaaaaggtt
     3421 attgtcaaag gcaaagaggt aggattgttt ttaactgaaa gtggtacaat tcacgcgatt
     3481 cacaatattt gtccgcataa acaaggacca ttgtcagagg gaacagtgag tggtgaatat
     3541 gtattctgtc cacttcatga tcaaaagatt gatttaaata ctgggattgt acaagaacct
     3601 gatgaaggtt gtgtagatgt ttatgaggta gaagttacag acgggaacgt atatatatgt
     3661 ctgtagataa atatggcaag gtttacttga taggtgcggg accaggtaat ccgaattatt
     3721 tgactaaaaa ggctgagcgt ttaatacgtg aagcggatgt tatactatat gatcgattag
     3781 tgaatccgtt aattttacaa tatgcgaatt tgactactga aattatcgat gttggtaaaa
     3841 agccgtatgc aaaacacatt cagcaagaaa aaataaatga atgtatcgtg gaagcagcac
     3901 gtcgatataa caaggttgtg aggcttaaag gtggcgaccc agcaatattt ggacgagttc
     3961 aagaagaagt tgacacacta aacaacttta atattgcgtt tgaaattgta cctggtgtga
     4021 catctgcgag tgctgcagtt gctacgatgc aaacaggatt aacgatgcgt gctgttgcta
     4081 agagtgtgac attttctaca ggtcacttta aagattcaga agaaaatgaa gtagatgtca
     4141 acgctttagt aaatggtggt acgttggcga tatatatggg agttaaacgt ttagggaaaa
     4201 ttatcgccca gatacaacag tacacagata ttgattatcc gatagcgatt gtctttcaag
     4261 catcgtgttt taacgagttt gttgttaagg ggcgtttaag taatattgtt gggaaactgg
     4321 agcattacgc aattgaggcg aaaccaggta tatgtattat aggcgaagta gtgggttata
     4381 ctgaaaatgt ctcaacaact tctaatccga ctcagcaatt ttatgtagta agtggttcta
     4441 gacatgacgc ccttatgctc tgtgaacatt tatatgacga aggttatggc tgtttgctaa
     4501 acccaaatga tacatcgaat ggcacatatc attcgtcgca atatgattac tatgatgcat
     4561 ttattaagca gcaagaaaat gtaacatata tttcaaccga tcgtgcagat gctaatacag
     4621 tgttatgtca ctaatttata aaaaataaat gaataagtaa ggtttcaacc gagagaatat
     4681 attcgtgttg aagccttatt tgtcgttgtg ccaaatttga acgatttaga ttggcaataa
     4741 gcacgaccat acatgattgt gtattatttc aatgaaatcc cgttattgat agggaaattc
     4801 cttaagtaat taggtgattc cctaatattt tatatttgtg aaaaaatgta caatctaatt
     4861 aaagcaatag tcttgggcat tttaaagtat ttaaaaacac gacatctaca catcgtattt
     4921 ttatacgtag gaggatataa atatgggaaa atttggattg aatttcttta agccaacaga
     4981 aaaatttaat gggaattggt cgatcctaga aagtaaaagt agagaatggg aaaaaatgta
     5041 cagagaacgt tggagccacg ataaagaagt aagaacaaca catggtgtta actgtacagg
     5101 ctcatgttct tggaaagtat ttgtgaaaaa tggtgtgatt acctgggaaa atcaacaaac
     5161 tgactatcca agttgtggtc cggatatgcc tgaatatgaa ccgagaggat gtccacgagg
     5221 tgcgtcattc tcttggtatg aatacagtcc gcttcgaatc aaatatccat atattcgtgg
     5281 aaaactctgg gatttatgga ctgaagcatt agaagaaaac tatggtaatc gcgttgctgc
     5341 atgggcgtct attgttgaaa atgaagacaa agccaaacaa tataagcaag cccgaggtat
     5401 gggagggcac gtgcgttcaa attggaaaga cgttacagag ataatcgcag cacaattact
     5461 gtatacaata aaaaaatatg gtccagatcg aatcgcagga tttacaccta ttccagcgat
     5521 gtcaatgatt agttatgcag caggtgctcg attcatcaat ttgcttggtg gtgaaatgct
     5581 tagtttttat gactggtatg cagatttacc acctgcctct ccacaaattt ggggagagca
     5641 aacagatgtg cctgaatcaa gtgactggta taacgcatca tacattatta tgtggggctc
     5701 taatgtacct ttaacacgta ctccggatgc acattttatg acagaagtcc gctataaagg
     5761 tacaaaagtc atttcagtag caccagatta cgcagaaaat gtgaaatttg cagataactg
     5821 gctagcaccg aatcctggtt cagatgctgc aattgctcaa gcaatgacac atgttatttt
     5881 acaagaacat tatgttaatc aacctaatga acgctttata aattacgcta aacaatatac
     5941 agatatgccg tttcttatca tgctggatga agatgaaaat ggatataaag cgggtcgatt
     6001 tttaagagcg agtgacttag gtcaaacaac agagcaaggc gaatggaagc cagttattca
     6061 tgatgcaatc agcgatagtt tagtagtacc taatggcaca atgggtcaac gttgggaaga
     6121 aggtaagaag tggaacttaa aactagaaac agaagatggt tctaaaatta accctacatt
     6181 atcaatggca gaaggtggat acgaattaga aacaattcaa ttcccatact ttgatagtga
     6241 tggagatggg atattcaatc gtccaattcc aactcgacaa gtcactttag caaatggtga
     6301 caaagtccgt attgctacaa tttttgactt aatggcaagt caatatggcg tgcgtcgttt
     6361 tgatcataaa ttagaatcaa aaggatacga cgatgcagaa tcaaaatata cacctgcttg
     6421 gcaagaagcc atttcaggcg taaaacaaag tgttgtcatt caagtagcga aagaatttgc
     6481 gcaaaacgct atcgatactg aagggcgttc aatgattatc atgggtgcgg gtattaacca
     6541 ttggtttaac tcagatacga tttatcgttc aatcttaaac ttagttatgt tatgtggctg
     6601 tcaaggtgtg aatggtggcg gttgggctca ctatgtggga caagaaaaat gtcgtccgat
     6661 tgaaggatgg agtactgtcg catttgcgaa agactggcaa ggaccaccac gtttgcaaaa
     6721 tggaacaagt tggttctatt ttgcaacaga ccaatggaaa tatgaagagt caaatgtaga
     6781 tcgtttaaaa tctccattag ctaaaacaga ggatttaaag catcaacacc cagctgatta
     6841 taatgtttta gcagctagac ttggttggtt accatcatat ccacaattta ataaaaatag
     6901 tttgttgttt gcagaagaag ctaaagatga aggcattgag tcgaatgagg caattttaaa
     6961 acgagcgata aatgaagtta agtcaaaaca aacgcaattt gcgatagaag atccggattt
     7021 gaaaaagaat catccgaaat cactgtttat atggcgctca aatctaatct caagttctgc
     7081 aaaaggtcaa gaatacttta tgaagcattt acttggcaca aaatcagggt tattagctac
     7141 accaaatgaa gatgaaaagc cagaagaaat tacgtggcgt gaggaaacaa cagggaaatt
     7201 agatttagtc gtttctttag atttcagaat gacagcaaca cctttatatt ctgacattgt
     7261 tttgccagca gcgacttggt atgagaagca tgatttgtca tctacagata tgcatccata
     7321 tgtacatcct tttaatccag ctattgatcc attatgggaa tcgcgttcag actgggatat
     7381 ttataaaacg ttggcaaaag cattttcaga aatggcaaaa gactatttac ctggaacgtt
     7441 taaagatgtt gtgacaactc cacttagtca tgatacaaag caagaaattt caacaccata
     7501 cggcgtagtg aaagattggt cgaagggtga aattgaagcg gtacctggac gtacaatgcc
     7561 taactttgca attgtagaac gcgactacac taaaatttac gacaaatatg tcacgcttgg
     7621 tcctgtactt gaaaaaggga aagttggagc acatggtgta agtttcggtg tcagtgaaca
     7681 atatgaagaa ttaaaaagta tgttaggtac gtggagtgat acaaatgatg attctgtgag
     7741 agcgaatcgt ccgcgtattg atacagcacg taatgtagca gatgcaatac taagtatttc
     7801 atctgctacg aatggtaaat tatcacaaaa atcatatgaa gatcttgaag aacaaactgg
     7861 aatgccgtta aaagatattt ctagcgaacg tgctgctgag aaaatttcgt ttttaaatat
     7921 aacttcacaa ccacgagaag taataccgac agcagtattc ccaggttcaa ataaacaagg
     7981 tcgacgatat tcaccattta caacgaatat agaacgtcta gtacctttta gaacattaac
     8041 aggacgtcaa agttattatg tggatcacga agttttccaa caatttgggg agagcttacc
     8101 agtatataaa ccgacattgc cgccaatggt atttgggaat agagataaga aaattaaagg
     8161 tggtacagat gctttggtac tgcgttattt aacgcctcat ggaaaatgga atatacactc
     8221 aatgtatcaa gataataagc atatgttgac actatttaga ggtggtccaa cggtttggat
     8281 atcaaatgaa gatgctgaaa aacacgatat ccaagataat gattggctag aagtgtataa
     8341 ccgtaatggt gttgtaacgg caagagcagt tatttcgcat cgtatgccta aaggtacaat
     8401 gtttatgtat catgcacaag ataaacatat tcaaacgcct gggtcagaaa ttacagatac
     8461 acgtggtggt tcacacaacg cgccgactag aatccatttg aaaccaacac aactagtcgg
     8521 aggatacgca caaattagtt atcactttaa ttattatgga ccaattggga accaaaggga
     8581 tttatatgta gcagttagaa agatgaagga ggttaattgg cttgaagatt aaagcgcaag
     8641 ttgcgatggt attaaattta gataaatgca taggatgcca tacgtgtagt gtgacatgta
     8701 aaaacacttg gacaaatcgt ccaggtgctg agtacatgtg gttcaataac gtagaaacga
     8761 agccaggtgt agggtatccg aaacgttggg aagaccaaga acactacaaa ggtggttggg
     8821 tactaaatcg taaagggaaa cttgaattaa aatctggaag tagaatttca aaaattgctt
     8881 taggtaaaat tttttataac ccagatatgc cattaataaa agattattat gaaccatgga
     8941 actataatta tgaacattta acaactgcga aatcagggaa gcattcgcca gttgctagag
     9001 cgtattcaga aattacaggg gataacattg aaattgaatg gggacctaac tgggaagatg
     9061 acttagcagg tggtcatgtt acaggcccaa aagatcctaa catccaaaaa atagaagaag
     9121 atattaaatt ccaatttgac gaaactttta tgatgtattt accacgttta tgtgaacatt
     9181 gcttgaatcc aagttgcgtt gcatcgtgcc cttcaggtgc tatgtataaa cgtgatgaag
     9241 atggcattgt attagttgat caagacgcat gtcgtggatg gcgttactgt atgacaggtt
     9301 gtccatataa gaaagtttac ttcaattgga aaacgaacaa agctgaaaaa tgtactttct
     9361 gtttcccgag aatcgaggcg ggtatgccta cggtatgctc tgaaacttgt acaggacgta
     9421 tgcgttactt aggtgtttta ttatatgatg cagatcgagt tcatgaagcg gcttcagcag
     9481 tagacgagaa aggtttatac gaaaaacaat tagatatatt cttaaatcca tttgacgaag
     9541 aagtcattgc tcaagctgaa aaagatggta ttggttatga ttggattgaa gctgcacaaa
     9601 actctccaat ttataagtta gcaattgaat ataaacttgc atttccgtta catcctgaat
     9661 ttagaacgat gccgatggtt tggtattgcc caccacttag cccaattatg agctattttg
     9721 aaggtaaaaa tacaactcaa aatccagatg cgattttccc agccattgaa gaaatgcgtt
     9781 tacctattga atacttagct aatattttca ctgcaggaga tacagagcca gtaaaaggag
     9841 ccctacaacg tatggcaatg atgagaagtt atatgagatc tcaagttact caacagccat
     9901 ttgacacgtc tcgattagag cgattaggta taaccgagcg ccaaactaaa gacatgtatc
     9961 gtttattagg actagctaaa tatgaagatc gatttgttat tccaacatca cacaaagaaa
    10021 cctatttaga tacgtatcac gcacaaggta gtacaggata caattacggc ggcgagcatt
    10081 ttggagataa ctgtgaaggc tgtggcgttg cagtaggttc agggaaaact ggtcaagaaa
    10141 tttataatga gaatttctat ggagggattt tccgtgatta atttcgataa ttttaaaaaa
    10201 tatcaagaaa gttttggata tatggcacaa cagttatgtt ttccagaaaa attaactttt
    10261 catccaaaaa cttttgaaga aacaatttcg aaagatcatc cagcatatga agatttagta
    10321 gcatttagaa atgtaatgat gacattttca ttatcagaaa tcaaagcgat ttatacagat
    10381 acatttgatt ttacgaaaaa agcaccatta tatatgacgt ataacaaatt tgatacgcaa
    10441 aaagaacgtg ggcaaatgtt agctaaatta aaggtattat atgaaatgtt tggactagaa
    10501 atggtagata atgaactttc tgattatcta ccgttgatgc ttcaattttt gcaagtggca
    10561 gaatggcgaa atgatccaag agctgaagag aatattcaat taatcattat gattattgag
    10621 gatggaacat atgtgatggc gaatgcactt ggtgaagata ataatccata tgcctatgtg
    10681 attcaagcac ttagaaaaac attgaaatta tgtttgacat caccaaaagg agtgaaacat
    10741 catgcttaat cagtttttat gggttatatt tccgtattta tgtttagttg tctttgtcgc
    10801 aggtcatatt gcacgttatc gttatgataa attttcttgg acagctaaat ctagtgaact
    10861 aatagaaaga aaaaggttga tgtggggaag tttattattt cacttaggta ttattccagt
    10921 gttttttgga cacgtagttg gattgttaat accgaaatca tggatggatg cagtgggggt
    10981 atctgagcat ttatatcata taggcgctgt ttatattggt agcatctttg gtataataac
    11041 tttgataggg atgtttttat taacagcgag acgtgttact acgaaaagta ttcgaagatt
    11101 aagttcagcg tcggatatat ttgtaaattt cttattatta cttatcgtat ttatgggatg
    11161 ttacgcaaca ttagtaacca atatacaaca tccagatttt aactacagaa cttcattatc
    11221 aatttggttt agacaattat ttatgttaaa accagatgcg tcattgatga gtggtgtgcc
    11281 gattgcattt aaaatgcata tattgttagg tttcactatt atggcttgtt ggccatttac
    11341 acgtttggta catgtttgga gtgtaccttt gacatatata aatcgcagat atattattta
    11401 ccgtaagaat aaagtgtaag cataagaggt gaaaggattt gacaccagaa gcaataattg
    11461 aagatcgtag atttcaagag acattagata aaatacgcaa agaagagggt tatgattttg
    11521 cggcaatcgc tttctatgaa tctaacaaac catcatctcc aataaaatgg cactatgttt
    11581 caggtaataa aaataatcga tttaaattga ttattttaag aaaaggtcgt ggattagctg
    11641 gaactgttat gaaaacagga aagcgtatgg taatagccaa tgttggttta gctttagggc
    11701 ctgaagaaaa gattgactat ccaattttat tgagcgaatc attaacagca gttttagcag
    11761 tgccgctttg gtataagaat caagtgtatg gcgtactatt atttggtcaa agagatggtc
    11821 gtcctttacc aaaaatattt gataatgatg atattcaacg taagtttggt attttcaatg
    11881 atgataaata atgagatgaa ttagggtgta agtcatgatt aatgaggaca gtatacagtt
    11941 agatacttta ttgaaaaaat attatgagca ttcaatcgaa aagatagttt ttgctgatga
    12001 taacggcaaa attattgcaa tgaatgatgc tgctaaagat atattatctg aagaagataa
    12061 ttacagtgct gtggcgaatg cgatttgtca cagatgtgaa gggtatacaa atgcctatga
    12121 tgtacaatca tgtaaagact gtttcttaga atcgatgcaa gtacaagcta cgaatttcca
    12181 agtatttatg aagacgaaag atcaaaaagt gatgccattt acagcaacat atcagttgat
    12241 tgatcaagac aggggaattc atgcatttac gcttcaaaat gtttcatcac aaattgaaca
    12301 gcaagagaaa ttacatcaac aacgtatgat gcgtaaaaca atctcagccc aagaaaatga
    12361 aagaaagcga atttctagag agttacatga tagtgtgatt caagaaatgc tcaatgtaga
    12421 tgttcaattg agattattaa aatatcaaga agatacaact aagcttttgg aagacgcaga
    12481 aaatattgaa tatattgtcg ctaaattaat tgatgatata cgtaatatgt cggttgaatt
    12541 aagacccgct tctttggatg atctaggcct tgaagcagct tttaaatcat attttaaaca
    12601 gtttgaagaa aattatggaa taaaaatcat atatacatca aatattaaaa atactcgctt
    12661 tgatagtgat attgaaacgg ttgtttatcg agtagtgcaa gaagctattc taaatgcgtt
    12721 gaaatacgct gatgtaaatg aaataaatgt aggtatccgt caaactgggc gccatttggt
    12781 agcagaagtg atagatgcgg gaaatggttt tgatccaagt tctaagccta aaggttctgg
    12841 actcggttta tatggaatga atgagcgagc tgagttagtc agcggtagcg tcaatattga
    12901 aacgaaaatt ggtgagggta ccaatgttac attgaacatt ccaatttgaa acatacattg
    12961 ggggaataaa attgaaaata gtcattgccg atgatcacgc tgttgtccgt acggggttct
    13021 ctatgatttt aaattatcaa aatgatatgg aagttgttgc aacggctgca gatggcgtcg
    13081 aagcttacca aaaagtaatg gaatataaac ctgatgtgtt actaatggat ttaagtatgc
    13141 caccaggtga gtcaggtctt atcgctacga gtaaaattgc tgacagtttt cctgaaacta
    13201 aaatactaat attaacaatg tttgatgatg aggagtattt gttccatgtg ttgcgtaatg
    13261 gtgcgaaagg ttacatattg aaaaatgcac ctgatgaaca attattgtta gctattcgaa
    13321 ctgtatataa aggtgaaaca tatgtagata tgaaacttac aacatcttta gtgaatgaat
    13381 ttgtatctaa ttcaaatcag gacactgcaa acacaacaga tccttttaaa atcttatcaa
    13441 aacgagaact agaaatattg ccacttattg ccaaaggtta cgggaataaa gaaattgcag
    13501 agaaattatt tgtatctgtg aaaacagtag aagcacataa gacgcatatt atgacaaagc
    13561 ttggcttaaa gagtaaacct gagctagttg aatatgcatt gaaaaagaaa ttattagagt
    13621 tttagagttt gaaattaata taattcagta taaaagcgcg agaggaaatg attatcttct
    13681 ggcgcttttg tatgtttcga aatatatttt tttataaatc gaatggaacc taatcctttc
    13741 tcctttcggt aacctatact aattcgcttt gcaaaggtat aacttttatt atagttattg
    13801 atttagagaa taaatgatta gttttctact ttgaacaatt attttagttg gtgtattcag
    13861 tgttgtagca tctgtatgag gattaaaaat tgtcgctctt attcacattg ctcaaaaaat
    13921 aattaacagc ttctgaaatg tatgatgcta aattttgatt atcatatgaa ttaaagtaat
    13981 ctttgaaacg cgtatcatct tcatatgttt tagcaataca gcataatgtt tcatcatcaa
    14041 aatcagcgat agtgttcata aatgccttcc actcaaatac gagatcacta caatcttgga
    14101 ttggataatg actcaaagac atatgattaa atttattgaa gatagttgtc aatttatgtc
    14161 tgatatcctg cgattgtaag ctgtctttac gtctaataaa tgattgatat gctttcgtat
    14221 gaccatattt tatagatgct tctttatcat attgtgactg tagtgtcttg tttaaaatgc
    14281 tggtatctat tagagagagt cctttaataa attcacatac agagcgtcta attaattgta
    14341 agtcagaaat ctttttatct aacttgctta tttgctctga taataacttt cgcaattgtt
    14401 cattatcata cgaaatgtat tgttttattt tagcgatatc aaaatcaaaa gacttgagta
    14461 ttaaaatctt ttgtaatttt tctaagtctt gctgtttata aacgcgataa tttttatcat
    14521 ttttatctgg aactaataat cctatttcat cgtaataatg taaagttctt ttagttacac
    14581 ctgtaatttc aataatatct tttaaagtgt atgtgtatgc caaaattcag tcacctcatt
    14641 tattattttt tgtgctggaa gtgttgtagc aacttcaata ggtatgtttg ttttttgtaa
    14701 aaagtatttt accaagtgat agccacatgc atacccggca gcatgagata ggccaacgat
    14761 aggcctacct tgaagtttat taatatcatc accatagaga taaggcatcg attcaaatat
    14821 atgctttaaa tgtaaatgat tataaatagt atttttaatt tttacgttat cacgactcca
    14881 attagtattt gttacccacg gtccaatatg cgctttacca tacaatgatt ctacatagtt
    14941 ttcagctaat ccttctgcaa taatcaattc aatcaaactt ccgccatccc aatcaatata
    15001 ttgatagcgc atattatgat ttacttcatg tgcaattaag cttttcatag aattaattgt
    15061 acttgtactc ggcactaaat aaatcataat ataacctggt atgccaccat caccaccgcg
    15121 atttttattt agatacataa gtggtttttg gctatctcca agtaatactg tgaaatgata
    15181 gttagaaact tgagatgaga tactataatt tgaaaattga tctatagcaa cttttaaata
    15241 tttttcgcaa ttactccaga aatcattatt caggtattgt attgacattt ggtcagacat
    15301 gcgtaaatct ttgggagaaa tttgaaaggc atctaaaaat tgaatcgcac tcattgtctg
    15361 tttgtcatca ttataaatag gcatatgttg tacctcaaat ttctttttaa aaggtactaa
    15421 tatttcttta gtgaaaattg aatcacggtc gtttattggt gccttgagta tattcttata
    15481 gacggaatct gatctaataa tattgatttt atacatgata aacctcctta tgttgtcagc
    15541 ataaaggata acgtaacgtg attttcaagc agtaattgta actaattgaa aaaaataaga
    15601 aaagtatgtg agtgttccta aataatatga ttaaaatgat ggcgaataag tgtctaaaag
    15661 catcttaaag ggacattgta tagggtaaat cacttcataa ataagggaaa atccttatgt
    15721 tcactttttc acaatcataa taaaatatat atgtagtcaa tactttgtct atattgaatg
    15781 ttttcatata aatgaaagca tttttaaata acattgacct ctaatatata ggcagagtat
    15841 tgatatctat taaaaaataa atgattttga tgaaggtgaa acgtatgtac aaaacaaaag
    15901 gtggctttca acttacatta caaacattaa gtttagtggt tgggtttatg gcttggagta
    15961 taattgcgcc attaatgccc tttattaaac aagatgtcaa tgttactgaa ggtcaaatat
    16021 caatcatttt agcgatacca gttattttgg gatcagtgct ccgtgtgcca tttggttatt
    16081 taacaaatat tgttggtgct aaatgggtat tctttacaag ttttatcgta ttgttattcc
    16141 cgatattttt cttaagccaa gcacaaacgc cgggtatgtt aatggcatca ggatttttcc
    16201 ttggcgtagg tggcgcaatt ttctctgttg gtgttacatc agttcctaaa tatttcccaa
    16261 aagaaaaagt aggtctagca aatggtattt atggtatggg gaatatcggt acagcagttt
    16321 cttcattttt agcaccaccg atagcgggta ttattggttg gcaaacaaca gttagaagtt
    16381 acttaattat tatcgcttta tttgcattaa ttatgttcat ttttggtgac acacaagaac
    16441 gtaaaattaa agtgccatta atggcccaaa tgaaaacatt atctaaaaac tacaaattat
    16501 attacttaag ttattggtat ttcattactt ttggtgcttt tgtagcattt ggtattttct
    16561 tacctaacta cttagtaaat cattttggaa ttgacaaagt agatgctggt attcgttcag
    16621 gtgtattcat tgcgctggca acattcttga gaccaatagg tggtatacta ggtgataaat
    16681 ttaatgcagt taaagtactg atgattgatt ttgttattat gattatcggt gccgttattt
    16741 taggtatttc agaccatatc gcattattca ctgtaggctg tttaacaata agtatttgtg
    16801 caggtattgg taacggctta atttttaaat tagtaccatc ctacttctca aatgaagcag
    16861 gatcagcaaa tggtatcgta tcaatgatgg gtggtttagg aggattcttc ccaccgttgg
    16921 tcatcacata cgtagctaat ttaacaggtt caagccattt agcatttatt ttcttagcgg
    16981 tatttggatg cattgcatta tttaccatgc gccatttata tcaaaaagaa tatggttcat
    17041 tgaaacacag ttgatatgta atacatgaca ttcatttagt taaatacaaa gccttaatat
    17101 catgcacaat attcgtagca tgacattaag gctttagtag tgtattcatt ttaaatttaa
    17161 ctatgaaatt ggaatagatg ttgatgaagt agtttcattg cttggtttga ttttaggcaa
    17221 tgtaatggtt aataacccat tttcaaaact agcagtaata tgttgcttat caacagcttc
    17281 aaaatcaaat tgacgcatta atgattcgaa gttacgctca tctaaaatga gttgttcaga
    17341 tttgtatttt gcgcttctag tagcttgaat agtgagcgta ttattattga aatcgatact
    17401 aatatcttct ttatttacac ctgctagttc agcttctaag taatataatt cgtctgtttc
    17461 atatatattc gttacaaatg aaggtgttga aaagtaatta aatacttgtt tacctaaatc
    17521 tttaaatgta tcacttggat taccgttaaa aaagttttga ttctcgaatt gattgaagtt
    17581 caatatcatc acttccatta tagaaatttg tattacaaag ccattatatg agtagtatta
    17641 aaaaagataa agtaaaaggg gagataaaaa agttataaat gggtagattt gtgcctgata
    17701 aggaatcagg gcaaaagaac tgagcatcga gatttataca agcaataaag ataatgaata
    17761 aacatagcga tgcatacttc tgcccatcac cttatgatag ggagttactt tctgtcggaa
    17821 tagtcaaaat ttttagaaac aaaattccgc aaattattaa ttatatctga tgcttctcgt
    17881 tctgaaatgt tgaattcatt aaagacctta acagaaattt cagcaagagg gctttttatt
    17941 gctttacctt gttctgttag agaaatttgt aggtttcttt catctttctc ttcacgtgtt
    18001 cgaacaacgt aatctttctt ttctaatttc tttagtaatg gtgtcagtgt tccagaatct
    18061 aagaatacac gttcacctaa ttttttgatg ttaagttttt catcattttc aatcgccatt
    18121 aaaacaatgt aaccagtata ggttaaatca tattccttaa gatagtttgt gtatttttta
    18181 attatttctt ttgaagaaac ataaaataaa aaacacaact gcttgctaag atagctgttt
    18241 tctacataca tccaatcact ccttgttaaa ataaacaata ttatgaagtg taatagttgt
    18301 caagtactga tttcatcgca attattgaat tgtctataat tgaatttaaa tgttaaataa
    18361 tgcaaaatat atcgatatct tagtattttc atcggtaatt actgatatga ctgatttttt
    18421 aaattgaaaa aaatgtaatt gtatcatcct taattgttaa aagtagtcaa ttattttgat
    18481 agatagtaaa ataaaatttg ttattaaatt ttttaaaagc atcgtgtatg taattcgatt
    18541 tctataataa tattgagaat aaaaaaattg ttacaatctg gtaaaaactc tttattaaat
    18601 aaattgattc gttcatactg aatagataag caaaaaggag ttacataatg aagaagaaaa
    18661 aaaacgcgat taaagttatc attatattat ttatttcatt aattgttgca gtagcatttt
    18721 tctttggtct gaaaacatat caaggccata agaatattca actaattgac agttacttgg
    18781 aagagaaaaa tctaaaagat aaaattaaat cagaaaaaac agagtatagt gctaaaaaag
    18841 gtctcttcta taaggaagtt acgtttaaag atgaacctgg tgtaacttat gttgtgcaac
    18901 caatcagtac taataaagga ttatttgttg aaggctttga tacagagact aagaaaagtt
    18961 tgaaaactgc aaagcataaa tatttcaatc aaaattataa accatcaaaa tagtgaaacg
    19021 ttaaactggt gaaacagttt agtgccttca attttaatta tttgaggtaa acagtttttg
    19081 tatggaaatt agtgattttt gacatctata
//
LOCUS       DAOBBH010000035        19039 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.035, whole genome shotgun sequence.
ACCESSION   DAOBBH010000035 DAOBBH010000000
VERSION     DAOBBH010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19039
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.035"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            74..505
                     /gene="mraZ"
                     /locus_tag="P6L01_000892"
     CDS             74..505
                     /gene="mraZ"
                     /locus_tag="P6L01_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDP3029977.1"
                     /translation="MFMGEYDHQLDTKGRMIIPSKFRYDLNERFIITRGLDKCLFGYT
                     LDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKE
                     CTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFDF"
     gene            521..1456
                     /gene="rsmH"
                     /locus_tag="P6L01_000893"
     CDS             521..1456
                     /gene="rsmH"
                     /locus_tag="P6L01_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDP3029978.1"
                     /translation="MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLND
                     DGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLG
                     VSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
                     KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELS
                     AFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTP
                     KLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK"
     gene            1470..1871
                     /gene="ftsL"
                     /locus_tag="P6L01_000894"
     CDS             1470..1871
                     /gene="ftsL"
                     /locus_tag="P6L01_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDP3029979.1"
                     /translation="MAVEKVYQPYDEQVYNSIPKQQPQTKPEKKTVSRKVVVQLTKFE
                     KVLYITLITVIAMLSIYMLSLKMDAYDTRGKIADLDYKIDKQSSENSALQSEIKKNSS
                     YERIYEKAKKQGMSLENDNVKVVRSNGEAKN"
     gene            1852..4086
                     /locus_tag="P6L01_000895"
     CDS             1852..4086
                     /locus_tag="P6L01_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDP3029980.1"
                     /translation="MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNG
                     QDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRH
                     VVDKKETAKKLSTVIDMKPEEIEKRLSKKKAFQIEFGRKGTNLTYQDKLKIEKMNLPG
                     ISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIH
                     DIWGYIAPNTKKEKQPKRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAK
                     TGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDK
                     KYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERF
                     GFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKP
                     WFVNSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKSHAANYRIDGY
                     EVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAY
                     ELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNVSAKSLEP
                     VTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTMPDMSGWTKEDVIAFENLTNIKV
                     NLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNSTNNSDSNSDDKKKSDSKTDK
                     DKSD"
     gene            4378..5343
                     /locus_tag="P6L01_000896"
     CDS             4378..5343
                     /locus_tag="P6L01_000896"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDP3029981.1"
                     /translation="MIFVYALLALVITFVLVPVLIPTLKRMKFGQSIREEGPQSHMKK
                     TGTPTMGGLTFLLSIVITSLVAIIFVDQANPIILLLFVTIGFGLIGFIDDYIIVVKKN
                     NQGLTSKQKFLAQIGIAIIFFVLSNVFHLVNFSTSIHIPFTNVAIPLSFAYVIFIVFW
                     QVGFSNAVNLTDGLDGLATGLSIIGFTMYAIMSFVLGETAIGIFCIIMLFALLGFLPY
                     NINPAKVFMGDTGSLALGGIFATISIMLNQELSLIFIGLVFVIETLSVMLQVASFKLT
                     GKRIFKMSPIHHHFELIGWSEWKVVTVFWAVGLISGLIGLWIGVH"
     gene            5345..6694
                     /locus_tag="P6L01_000897"
     CDS             5345..6694
                     /locus_tag="P6L01_000897"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDP3029982.1"
                     /translation="MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLS
                     QDAHAKDLESMGISVVSGSHPLTLLDNNPIIVKNPGIPYTVSIIDEAVKRGLKILTEV
                     ELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPT
                     DYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDY
                     LICNYHQRQVIESEELKAKTLYFSTQQEVDGIYIKDGFIVYKGVRIINTEDLVLPGEH
                     NLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLA
                     TQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKS
                     VIEANNVEDAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY"
     gene            6710..8029
                     /locus_tag="P6L01_000898"
     CDS             6710..8029
                     /locus_tag="P6L01_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDP3029983.1"
                     /translation="MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQ
                     NKDDTSQQADFDEEIYLINKDFKKEESNDENNGSASSHANDNNIDDSTDSNIENEDYR
                     YNQEIDDQNESNGIAVGNEQPQSAPKEQNSDSNDEETVTKKERKSKVTQLKPLTLEEK
                     RKLRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLG
                     VKNDSRMYTFSKKNAINDLEEDPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYL
                     PLLENGKLLKGSNDVKINDAPVMDGFKGTKEDDMIKALSEMTPEVRRYIAEVTYAPSK
                     NKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLSRDSSGKLKTRGYIDLSVGAS
                     FIPYRGNTSSQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN"
     gene            8135..9547
                     /gene="ftsA"
                     /locus_tag="P6L01_000899"
     CDS             8135..9547
                     /gene="ftsA"
                     /locus_tag="P6L01_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDP3029984.1"
                     /translation="MEEHYYVSIDIGSSSVKTIVGEKFHNGINVIGTGQTYTSGIKNG
                     LIDDFDIARQAIKDTIKKASIASGVDIKEVFLKLPIIGTEVYDESNEIDFYEDTEING
                     SHIEKVLEGIREKNDVQETEVINVFPIRFIVDKENEVSDPKELIARHSLKVEAGVIAI
                     QKSILINMIKCVEACGVDVLDVYSDAYNYGSILTATEKELGACVIDIGEDVTQVAFYE
                     RGELVDADSIEMAGRDITDDIAQGLNTSYETAEKVKHQYGHAFYDSASDQDIFTVEQV
                     DSDETVQYTQKDLSDFIEARVEEIFFEVFDVLQDLGLTKVNGGFIVTGGSANLLGVKE
                     LLSDMVSEKVRIHTPSQMGIRKPEFSSAISTISSSIAFDELLDYVTINYHDNEETEED
                     VIDVKDKDNESKLGGFDWFKRKTNKKDTHENEVESTDEEIYQSEDNHQEHKQNHEHVQ
                     DKDKDKEESKFKKLMKSLFE"
     gene            9580..10752
                     /gene="ftsZ"
                     /locus_tag="P6L01_000900"
     CDS             9580..10752
                     /gene="ftsZ"
                     /locus_tag="P6L01_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDP3029985.1"
                     /translation="MLEFEQGFNHLATLKVIGVGGGGNNAVNRMIDHGMNNVEFIAIN
                     TDGQALNLSKAESKIQIGEKLTRGLGAGANPEIGKKAAEESREQIEDAIQGADMVFVT
                     SGMGGGTGTGAAPVVAKIAKEMGALTVGVVTRPFSFEGRKRQTQAAAGVEAMKAAVDT
                     LIVIPNDRLLDIVDKSTPMMEAFKEADNVLRQGVQGISDLIAVSGEVNLDFADVKTIM
                     SNQGSALMGIGVSSGENRAVEAAKKAISSPLLETSIVGAQGVLMNITGGESLSLFEAQ
                     EAADIVQDAADEDVNMIFGTVINPELQDEIVVTVIATGFDDKPTSHGRKSGSTGFGTS
                     VNTSSNATSKDESFTSNSSNAQATDSVSERTHTTKEDDIPSFIRNREERRSRRTRR"
     gene            11012..11803
                     /gene="pgeF"
                     /locus_tag="P6L01_000901"
     CDS             11012..11803
                     /gene="pgeF"
                     /locus_tag="P6L01_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDP3029986.1"
                     /translation="MNDNFKKQPHHLIYEELLQQGITLGITTRGDGLSDYPKNAFNMA
                     RYIDDRPYNITQHQLQLAEEIAFDRKNWVFPIQTHENKVACITKDDIGTNIDTLTDAL
                     HGIDAMYTYDSNVLLTMCYADCVPVYFYSTKHHFIALAHAGWRGTYTEIVKEVLKHVN
                     FDLKDLHVVIGPSTSSSYEINDDIKNKFETLPIDSANYIETRGRDRHGIDLKKANAEL
                     LNYYGVPKENIYTTAYATSEHLELFFSYRLEKGQTGRMLAFIGQQ"
     gene            11827..12495
                     /locus_tag="P6L01_000902"
     CDS             11827..12495
                     /locus_tag="P6L01_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDP3029987.1"
                     /translation="MKDNLQQISTQINDKSEKNNFSTKPNVIAVTKYVTIERAKEAYE
                     AGIRHFGENRLEGFFQKKEALPSDAVIHFIGSLQSRKVKDVINDVDYFHALDRLSLAK
                     EINKRAEHKIKCFLQVNVSGEASKHGIALEDVDQFIDDLKKYDKIEIVGLMTMAPLTD
                     DEAYIRSLFKQLRLKKEEIQRLNLEYAPCDELSMGMSNDYLIAVEEGATFVRIGTKLV
                     GEEE"
     gene            12492..13055
                     /locus_tag="P6L01_000903"
     CDS             12492..13055
                     /locus_tag="P6L01_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDP3029988.1"
                     /translation="MSHLALKDLFSGFFVIDDEEEVEVPDKQQQVNEAPAKEQSQQTT
                     KQNAIKSVPQKSASRYTTTSEERNNRMSNYSKNNSRNVVTMNNATPNNASQESSKMCL
                     FEPRVFSDTQDIADELKNRRATLVNLQRIDKVSAKRIIDFLSGTVYAIGGDIQRVGTD
                     IFLCTPDNVEVAGSITDHIENMEHSFD"
     gene            13067..13357
                     /locus_tag="P6L01_000904"
     CDS             13067..13357
                     /locus_tag="P6L01_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDP3029989.1"
                     /translation="MDINVLATIFKFILFVVEIYYFGMIIYFFTSWVPSIRETKVGYF
                     LAKIYEPFLQPFRKVIPPIGIIDISSIAAIIVLVLFQKGLLQIFNWILIQLQ"
     gene            13467..14246
                     /locus_tag="P6L01_000905"
     CDS             13467..14246
                     /locus_tag="P6L01_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDP3029990.1"
                     /translation="MINIDIYQHFRQEEYELIDQLTDKCDQAEQHYAPVLTHFLDPRG
                     QYILEVICGSYEDLNVSFYGGPNAERKRAIISPNYYEPKESDFELTLMEIEYPEKFVT
                     LKHQHILGTLMSLGIEREQVGDIIVNERIQFVLTSRLESFIMLELQRIKGASVKLYTI
                     PVTDMIQSNENWKNESATVSSLRLDVVIKEMIRKSRTIAKQLIEKKRVKVNHTIVDSA
                     DFQLQANDLISIQGFGRAHITDLGGKTKKDKTHITYRTLFK"
     gene            14270..14887
                     /locus_tag="P6L01_000906"
     CDS             14270..14887
                     /locus_tag="P6L01_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDP3029991.1"
                     /translation="MPFTPNEIKNKEFSRVKNGLEPTEVANFLEQLSTEIERLKEDKK
                     QLEKVIEERDTNIKSYQDVHQSVSDALIQAQKAGEETKQAAEKQAEAIIAKAEAQANQ
                     MVGDAVEKARRLAFQTEDMKRQSKVFRSRFRMLVEAQLDLLKNEDWDYLLNYDLDAEQ
                     VTLENIHHLHENDLKPDEVAANAQNNASNTPDNNQQSNDSETTKK"
     gene            15108..17861
                     /gene="ileS"
                     /locus_tag="P6L01_000907"
     CDS             15108..17861
                     /gene="ileS"
                     /locus_tag="P6L01_000907"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDP3029992.1"
                     /translation="MDYKETLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKN
                     KGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIE
                     QALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYE
                     AAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYHDKRSASIYVAFDVKDDK
                     GVVDADAKFIIWTTTPWTIPSNVAITVHPELKYGQYNVNGEKYIIAEALSDAVAEALD
                     WDKASIKLEKEYTGKELEYVVAQHPFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDD
                     YIVGQKYELPVISPIDDKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITH
                     SYPHDWRTKKPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGE
                     WVISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREAKDLLPEGF
                     THPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRGWFNSS
                     ITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSS
                     TDYLADVRISDEILKQTSDVYRKIRNTLRFMLGNINDFNPDTDSIPESELLEVDRYLL
                     NRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRS
                     MQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKW
                     RTFMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALHQLFIVS
                     QVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVDELTHLCPRCQQVVK
                     SLV"
     gene            18152..18949
                     /locus_tag="P6L01_000908"
     CDS             18152..18949
                     /locus_tag="P6L01_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDP3029993.1"
                     /translation="MYHNSNANFVNGITLNVRDKNELKPFYEDVLGLNIINETLTSIQ
                     YEVGQNNHVITLVELQNGREPLMSEAGLFHIAIKLPQISDLANLLIHLSEYDIPVNGG
                     IQPASLSLFFEDPEGNGFKFYVDKDEAQWTRQNDLVKIDIRPLNVPRLVSHATKLLWL
                     GIPDDAIIGALHIKTIHLSEVKEYYLDYFGLEQSAYMDDYSIFLASNGYYQHLAMNDW
                     VSATKRVENFDTYGLAIVDFHYPETTHLNLQGPDGIYYRFNHIEVED"
BASE COUNT         7020 a         2611 c         3701 g         5707 t
ORIGIN      
        1 cacaaatttt gtataaatag tggtggatag tggggagatg tggtaaatta tatataaggt
       61 gaggtgataa aaaatgttca tgggagaata cgatcatcaa ttagatacaa aaggacgtat
      121 gattataccg tccaagtttc gttatgactt aaatgagcgt tttattatca caagaggcct
      181 tgataaatgt ttattcggtt acactctaga cgaatggcaa cagattgaag agaaaatgaa
      241 aaccttacct atgacaaaaa aagacgcacg taagtttatg cgtatgttct tctctggtgc
      301 tgttgaagta gaacttgata agcaagggcg tattaacatc cctcaaaact tgaggaaata
      361 cgctaattta actaaagaat gtacagtaat cggtgtttca aatcgtattg agatttggga
      421 tagagaaact tggaatgatt tctatgaaga atctgaagaa agtttcgaag atattgctga
      481 agatttaata gattttgatt tttaaaatgg aggaattgaa gtgtttcatc atatcagcgt
      541 tatgttaaac gaaaccattg attatttaaa tgtaaaagaa aatggtgtgt acattgactg
      601 tacgctaggt ggagcgggac atgcccttta tttactaaat caattaaatg acgacggaag
      661 attaatagca atcgatcaag accaaactgc aattgataat gctaaagagg tattaaagga
      721 tcatttgcat aaggtgactt ttgttcatag caacttccgt gaattaactc aaatattaaa
      781 agacttaaac attgaaaaag tagatggaat ttattacgac ttgggtgttt caagcccaca
      841 actcgacatt ccagaacgag gattcagtta tcaccatgac gcaacattag acatgcgtat
      901 ggaccaaaca caagaactaa cagcatatga aattgttaac aattggtcat atgaagcgtt
      961 agtgaagatt ttttatcgtt atggcgagga gaaattttca aaacagatag ctcgaagaat
     1021 cgaagcacat cgtgaacaac aaccaataac aacaacatta gaattagttg acattataaa
     1081 agaaggtatt cctgcaaaag caagaagaaa aggcggacat cctgcaaaac gagtatttca
     1141 agcactacga attgcagtaa acgatgaatt gtcagctttt gaagattcaa tagaacaagc
     1201 gattgaatta gtgaaagtag acggcaggat ttcggtaatc actttccatt ctttagaaga
     1261 tcgtttatgt aaacaggtgt tccaagaata tgaaaaaggt ccagaggtac caagaggatt
     1321 gccagttata ccagaagcat atacacctaa gttaaagcgt gttaatcgta aaccgattac
     1381 cgctacagaa gaagatttag atgacaataa cagagcacga agcgcgaaat tacgtgtagc
     1441 tgaaatactt aaataaggag caatttataa tggctgtaga aaaagtgtac caaccatatg
     1501 acgaacaagt ttataatagt ataccgaagc aacaaccaca aactaagccc gaaaagaaga
     1561 ctgtttcgag aaaagtggtt gtacaattaa ctaaatttga aaaagtttta tacataactt
     1621 tgattactgt aattgctatg ttaagtattt atatgctatc tttaaaaatg gatgcgtatg
     1681 atacgcgagg aaagattgca gatttagatt ataaaataga taaacaatca agtgaaaaca
     1741 gtgctttaca atctgaaatc aaaaagaatt cttcttatga acgcatatac gaaaaggcta
     1801 agaaacaggg gatgagcctt gagaacgata atgtaaaggt agtgcgtagt aatggcgaag
     1861 caaaaaatta aaattaaaaa aaataaaata ggggcagtcc tacttgttgg tttattcgga
     1921 ctgctctttt ttatattggt tttaagaatt tcatatatca tgattactgg acattctaat
     1981 ggtcaagatt tagtcatgaa ggcaaatgaa aagtatttag ttaagaatgc acaacaacca
     2041 gaacgaggaa agatatatga tcgtaatggt aaagtgctag cagaagatgt agaaagatat
     2101 aaacttgttg cagtaataga taaaaaggcg agtgccaatt ctaaaaaacc taggcatgta
     2161 gttgataaaa aagagactgc aaagaaatta tctacagtca ttgatatgaa gccagaggaa
     2221 attgaaaaga gacttagtaa aaagaaagct ttccaaattg aatttggacg caaaggaaca
     2281 aatttaacgt atcaggacaa attgaaaata gagaaaatga atttgcctgg tatttcttta
     2341 ttgcctgaaa cagaacgctt ttatccaaat ggcaattttg catcacactt aattggtaga
     2401 gctcagaaaa atccggatac tggtgaactt aaaggtgcac ttggagttga aaagattttt
     2461 gatagttatt taagtggatc taaaggatca ttgagatata ttcatgatat ttggggatat
     2521 atcgcaccaa atactaaaaa agagaagcag cctaaacgtg gtgatgatgt ccatttaaca
     2581 atcgattcaa atattcaagt atttgttgaa gaagctttag atggcatggt tgaaagatac
     2641 cagccgaaag atttatttgc ggttgtcatg gatgccaaaa ctggagaaat tttagcatac
     2701 agtcagcgac caacatttaa tcctgaaact ggtaaagact ttggtaaaaa gtgggcaaat
     2761 gacctttatc aaaacacata cgagcctgga tcaacattta aatcatatgg gttagcagct
     2821 gctattcaag aaggtgcttt tgatcctgat aagaaatata aatctggaca tagagatatt
     2881 atgggttcac gtatttcaga ctggaataga gtcggttggg gtgaaatccc aatgtcactc
     2941 ggatttactt attcatctaa tacattgatg atgcatttac aagatttagt tggtgcagac
     3001 aaaatgaaat cttggtatga acgatttgga tttggaaaat caactaaagg tatgtttgat
     3061 ggagaagcac ctggtcaaat tggatggagt aatgagttgc aacaaaaaac gtcatcattt
     3121 ggtcaatcga caacagtaac acctgttcaa atgttacagg cgcaatcagc gttctttaat
     3181 gatggtaata tgttaaaacc atggtttgtg aatagcgttg aaaatcctgt tagtaaaaga
     3241 caattttata aaggtcaaaa acaaatcgca ggcaaaccaa taacaaaaga tactgctgaa
     3301 aaagttgaaa agcaattgga tttagttgtg aatagtaaga agagtcacgc tgcaaactat
     3361 cgtattgatg gttatgaggt cgaaggtaag actggtacag cacaagtcgc tgcacctaat
     3421 ggtggtggat acgttaaagg tccaaaccca tattttgtaa gttttatggg tgacgcaccg
     3481 aagaaaaatc ctaaagttat tgtatacgct ggtatgagct tggcacaaaa aaatgaccaa
     3541 gaagcttatg aattaggtgt tagtaaagcg tttaaaccaa taatggaaaa tactttgaaa
     3601 tatttaaatg taggtaaatc aaaagatgac acatctaatg cagagtatag taaagtgcca
     3661 gatgttgaag gtcaagacaa acaaaaagct attgataatg tgagtgcaaa atcattagaa
     3721 ccagttacta tcggttctgg cacacaaata aaagcacaat ctataaaagc agggaataaa
     3781 gtcttacctc atagtaaagt actgttatta acagacggag acttaactat gcctgacatg
     3841 tcaggatgga cgaaagaaga tgtcattgct tttgaaaacc taacaaatat taaagtaaat
     3901 ttaaaaggta gcggttttgt gtcccaccaa tcaattagta agggacaaaa acttactgaa
     3961 aaagataaaa tagacgtaga attttcatca gagaatgtag acagcaattc gacgaataat
     4021 tctgattcaa attcagatga taagaagaaa tctgacagta aaactgacaa ggataagtcg
     4081 gactaacaag aatggctaca ttaaataagg gtgtattgtc actatttgtt tttactaaag
     4141 taatgctgtg atagaaaaaa acatcttcat ttaatcttta taaataagtg ttgatagaga
     4201 attttaaggt agacgaggtc agattaactt gtttgttata ggcaaacttt ttatactggc
     4261 ctcttatgcc gtaaaaaaga aaaattataa attttgatta caactttgtt tatttaattg
     4321 aaaaattgat aatatattag tggttaaaaa agtctggatt aagaagagga gatagttatg
     4381 atttttgtat atgcgttatt agcgctagtg attacatttg ttttggtacc tgttttaata
     4441 cctacattaa aaaggatgaa atttggtcaa agtattcgag aagaaggtcc acaaagccat
     4501 atgaagaaga ctggtacacc aacgatgggt ggactaacat ttctattaag tattgtgata
     4561 acgtctttgg tggctattat atttgtagat caagctaatc caatcatact gttattattt
     4621 gtgacgattg gttttgggtt aattggtttt atagatgatt atattattgt tgttaaaaag
     4681 aataaccaag gtttaacaag taaacagaag tttttggcgc aaattggtat tgcgattatt
     4741 ttctttgttt taagtaatgt gtttcatttg gtgaattttt ctacgagcat acatattcca
     4801 tttacgaatg tagcaatccc actatcattt gcatatgtta ttttcattgt tttttggcaa
     4861 gtaggttttt ctaatgcggt aaatttaaca gatggtttag atggattagc aactggactg
     4921 tcaattatcg gatttacaat gtatgccatc atgagctttg tgttaggaga aacggcaatt
     4981 ggtattttct gtatcattat gttgtttgca cttttaggat ttttaccata taacattaac
     5041 cctgctaaag tgtttatggg agatacaggt agcttagctt taggtggtat atttgctacg
     5101 atttcaatca tgcttaatca ggaattatca ttaattttta taggtttagt attcgtaatt
     5161 gaaacattat ctgttatgtt acaagtcgct agctttaaat tgactggaaa gcgtatattt
     5221 aaaatgagtc cgattcatca tcattttgaa ttgataggat ggagcgaatg gaaagtagtt
     5281 acagtatttt gggctgttgg tctgatttca ggtttaatcg gtttatggat tggagtgcat
     5341 taagatgctt aattatacag ggttagaaaa taaaaatgta ttagtcgtcg gtttggcaaa
     5401 aagtggttat gaagcagcta aattattaag taaattaggt gcgaatgtga ctgtcaatga
     5461 tgggaaagac ttatcgcaag atgctcatgc aaaagattta gaatctatgg gtatttctgt
     5521 tgtaagtgga agtcatccat taacattgct tgataataat ccaataattg ttaaaaatcc
     5581 tggaatacct tatacagtat ctattattga tgaagcagtg aaacgaggtt tgaaaatttt
     5641 aacagaagtt gagttaagtt atctaatctc tgaagcacca atcatagctg taacgggtac
     5701 aaatggtaaa acgacagtta cttctctaat tggagatatg tttaaaaaaa gtcgcttaac
     5761 tggaagatta tccggcaata ttggttatgt tgcatctaaa gtagcacaag aagtaaagcc
     5821 tacagattat ttagttacag agttgtcgtc attccagtta cttggaattg aaaagtataa
     5881 accacacatt gctataatta ctaacattta ttcggcgcat ctagattacc atgaaaattt
     5941 agaaaactat caaaatgcta aaaagcaaat atataaaaat caaacggaag aagattattt
     6001 gatttgtaat tatcatcaaa gacaagtgat agagtcggaa gaattaaaag ctaagacatt
     6061 gtatttctca actcaacaag aagttgatgg tatttatatt aaagatggtt ttatcgttta
     6121 taaaggtgtt cgtattatta acactgaaga tctagtattg cctggtgaac ataatttaga
     6181 aaatatatta gcagctgtgc ttgcttgtat tttagctggt gtacctatta aagcaattat
     6241 tgatagttta actacatttt caggaataga gcatagattg caatatgttg gtactaatag
     6301 aactaataaa tattataatg attccaaagc aacaaacacg ctagcaacac agtttgcctt
     6361 aaattcattt aatcaaccaa tcatttggtt atgtggtggt ttggatcgag ggaatgaatt
     6421 tgacgaactc attccttata tggaaaatgt tcgcgcgatg gttgtattcg gacaaacgaa
     6481 agctaagttt gctaaactag gtaatagtca agggaaatcg gtcattgaag cgaacaatgt
     6541 cgaagacgct gttgataaag tacaagatat tatagaacca aatgatgttg tattattgtc
     6601 acctgcttgt gcgagttggg atcaatatag tacttttgaa gagcgtggag agaaatttat
     6661 tgaaagattc cgtgcccatt taccatctta ttaaagggtg tgagtattga tggatgataa
     6721 aacgaagaac gatcaacaag aatcaaatga agataaagat gaattagaat tatttacgag
     6781 gaatacatct aagaaaagac ggcaaagaaa aaggtcaaag gctacacatt tttctaatca
     6841 aaataaagat gatacatctc aacaagctga ttttgatgaa gaaatttact tgataaataa
     6901 agacttcaaa aaagaagaaa gcaatgatga aaataatggt tctgcttcta gtcatgcgaa
     6961 tgataataat atcgatgatt ctacagactc taatattgaa aatgaggatt atagatataa
     7021 tcaagaaatt gacgaccaaa atgaatcgaa tggaattgca gtcggcaacg aacaacctca
     7081 atcagctcct aaagaacaaa atagcgactc gaatgatgag gaaacagtaa cgaaaaaaga
     7141 acgaaaaagt aaagtaacac aattaaagcc attaacactt gaagaaaagc ggaagttaag
     7201 acgtaagcga caaaaacgaa tccaatacag tgttattaca atattagtat tgttgattgc
     7261 tgttatatta atttacatgt tttcaccact tagtaaaatt gcgcatgtaa atataaatgg
     7321 aaataatcac gttagtactt caaagataaa caaagtttta ggtgttaaaa atgattcgag
     7381 gatgtatacg tttagtaaaa aaaatgctat taatgatctc gaagaggatc cattaatcaa
     7441 aagtgttgag atacacaagc aattaccaaa cacattaaac gtagatatca cagaaaatga
     7501 aattattgct ttagtgaaat ataaaggtaa atatttacct ttattagaaa atggtaaatt
     7561 gcttaaaggt tcaaatgatg tcaaaattaa tgatgcacct gtcatggatg gtttcaaagg
     7621 tacaaaagaa gatgatatga ttaaggcgtt atctgaaatg acacctgaag ttagacgata
     7681 tattgccgaa gtgacatacg ccccaagtaa aaacaaacaa agcagaattg aattgtttac
     7741 gacagatgga cttcaagtaa tcggtgatat ttcgacgata tctaagaaaa tgaaatatta
     7801 tccgcagatg tcacaatcat tatcaaggga tagttcgggt aaactaaaaa caagaggcta
     7861 tattgattta tcagtcggtg cttcatttat cccataccgt ggaaacacgt ctagtcaatc
     7921 agaaagcgat aaaaatgtga ctaaatcatc tcaagaggaa aatcaagcaa aagaagaatt
     7981 acaaagcgtt ttaaacaaaa ttaacaaaca atcaagtaag aataattaaa aaattttgat
     8041 attgtctatg tttatagttc acaagccatt caacgtattg taaactaagg atagtgtatt
     8101 tttttaatag taatttgtca ggaggtgcct atctatggaa gaacattact acgtaagtat
     8161 tgatattgga tcatcaagcg taaaaacaat agtaggcgag aaatttcaca atggtataaa
     8221 tgtgataggt acaggacaaa cctacacgag cggtataaaa aatggtttaa ttgatgattt
     8281 tgatattgcg cgacaagcaa tcaaagacac aattaaaaag gcatcaatcg cttcgggtgt
     8341 tgatattaaa gaagttttcc tgaaattacc tatcattgga acggaagttt atgatgaatc
     8401 aaatgaaatc gacttttatg aggatacaga aatcaacggt tcacatatcg aaaaagtatt
     8461 agaaggtatt agagaaaaaa atgatgtgca agaaacagaa gtaattaatg tgttcccgat
     8521 tcgttttata gtcgataaag aaaatgaggt ttcagaccct aaagaattaa ttgccagaca
     8581 ttcattaaag gttgaagcag gcgtaattgc tattcaaaaa tcgattttaa ttaatatgat
     8641 taaatgcgta gaagcatgtg gtgttgatgt attagatgtt tactctgatg catataacta
     8701 tggttcaatc ctaacagcta ctgaaaaaga gttaggtgca tgtgtcattg atattggtga
     8761 agacgttacg caagttgctt tttatgaacg cggtgaatta gtagatgctg attctatcga
     8821 aatggcaggg cgtgatatta cagacgatat tgcacaagga ttaaacactt cttatgaaac
     8881 tgctgaaaaa gttaaacacc aatatggtca tgcattctat gattctgctt cagatcaaga
     8941 tatcttcact gttgaacagg ttgatagtga tgaaacagta cagtatactc aaaaagattt
     9001 gagtgacttt attgaagcgc gtgtagaaga aatattcttc gaagtatttg atgttttaca
     9061 agatttagga ttaacaaaag taaatggtgg gtttattgta actggtggat ctgcaaactt
     9121 acttggcgta aaagaattat tatcagatat ggtaagtgaa aaagttagaa ttcacacgcc
     9181 atcacaaatg ggaattagaa aacctgaatt ttcttcagca atttctacaa tttctagtag
     9241 tatcgctttt gatgagttat tagattatgt tacaattaat tatcatgata atgaagaaac
     9301 tgaagaagat gttattgatg tgaaagacaa agataacgaa tctaaattag gcggatttga
     9361 ttggtttaaa cgtaaaacaa acaaaaaaga tactcatgaa aatgaagtag agtcaacaga
     9421 tgaagaaatt tatcaatcag aagataatca tcaggaacat aaacagaatc atgaacatgt
     9481 tcaagacaaa gataaagata aagaagaaag taaattcaaa aaactaatga aatctctatt
     9541 tgaatgatta ttggccaata aaactaggag gaaatttaaa tgttagaatt tgaacaagga
     9601 tttaatcatt tagcgacttt aaaggtcatt ggtgtaggtg gtggcggtaa caacgccgta
     9661 aaccgaatga ttgaccacgg aatgaataat gttgaattta tcgctatcaa cacagacggt
     9721 caagctttaa acttatctaa agctgaatct aaaatccaaa tcggtgaaaa attaacacgt
     9781 ggtttaggag caggagctaa ccctgaaatc ggtaaaaaag ctgcagagga atctcgtgaa
     9841 caaattgaag atgcaatcca aggtgcagac atggtatttg ttacttctgg tatgggtggc
     9901 ggaactggta ctggtgcagc accagtcgtt gctaaaattg caaaagaaat gggcgcatta
     9961 actgttggtg ttgtaactcg tccatttagt tttgaaggac gtaaacgtca aactcaagct
    10021 gctgctggag tagaagctat gaaagctgca gtagatacat taatcgttat accaaatgac
    10081 cgtttattag atatcgttga caaatctacg ccaatgatgg aagcatttaa agaagctgat
    10141 aacgtgttac gccaaggtgt acaaggtatc tcagacttaa tcgctgtttc tggtgaagta
    10201 aacttagact ttgcagacgt taagacaatt atgtctaacc aaggttctgc attaatgggt
    10261 attggtgttt cttctggtga aaatagagcg gtagaagctg ctaaaaaagc aatctcttct
    10321 ccattacttg aaacatctat cgttggtgca caaggtgtgc ttatgaatat tactggtggc
    10381 gagtcattgt cattatttga agcacaagag gctgctgata ttgtccaaga tgctgcagat
    10441 gaagacgtta atatgatttt cggtacagtt attaatcctg aattacaaga tgagattgtt
    10501 gtaacagtta ttgcaactgg ttttgatgac aaaccaacat cacatggtcg taaatctggt
    10561 agcactggat tcggaacaag cgtaaatact tctagcaatg caacttctaa agatgaatca
    10621 ttcacttcaa attcatcaaa tgcacaagca actgatagtg taagtgaaag aacgcataca
    10681 actaaagaag atgatattcc aagcttcatt agaaatagag aagaaagacg ttcaagaaga
    10741 acaagacgtt aatcggttaa tatatataca caaataattc aacacaaatc atcagataac
    10801 atatctgatg atttttttac taatttttag aacatgtaga aggacattta agtttttcga
    10861 agtcattaaa agtgtttaag tatcgtgtca aaattaagtc aaaaattatt tgcgcaacat
    10921 tttaacttta aacataaatg ttatattata taattattaa ctttgtacag ttagacgaag
    10981 ataatttaaa tgaaatgatg gtgacgatcg agtgaatgat aattttaaaa agcaaccgca
    11041 tcatttaata tatgaagaat tattacaaca aggtattact ctaggtatta caactagagg
    11101 agatggttta agcgactatc ctaaaaatgc ttttaatatg gcgagatata ttgatgatcg
    11161 cccatataat attactcaac atcaattgca attagctgaa gaaattgcgt ttgatagaaa
    11221 aaattgggtg tttcccatcc aaacacatga aaataaagtc gcttgtatta caaaggatga
    11281 tataggcaca aatatagaca ctttaactga tgcgcttcat ggtattgatg cgatgtacac
    11341 atatgatagt aatgtcttat taacgatgtg ttatgcagac tgtgtaccag tatattttta
    11401 tagtacaaaa catcatttta ttgcattggc gcatgcaggt tggcgtggta cctatactga
    11461 aattgtaaaa gaagtgctaa aacatgtgaa ctttgatttg aaagacttac atgtcgttat
    11521 tggaccatct acatcatcaa gttatgaaat taatgatgat attaaaaata aatttgaaac
    11581 attgccaatt gatagtgcca actatattga aactagagga cgagatcgtc atggtattga
    11641 tttgaaaaaa gccaatgctg aattattaaa ttattatggt gttcctaaag aaaatattta
    11701 tacgacagcg tatgctacat ctgaacattt agaattattt ttctcttatc gattagaaaa
    11761 aggtcaaaca ggacgcatgt tagcattcat tggtcaacag taaacaagga ggagatatgt
    11821 ttgcgtgtga aagataattt acaacaaatc tcaacacaaa ttaatgacaa aagtgaaaaa
    11881 aataattttt caacaaaacc aaacgtgatt gcagttacaa aatatgttac aatagagcga
    11941 gctaaagaag cgtatgaggc tggaataaga cattttggtg agaatagatt ggaaggcttt
    12001 tttcaaaaga aagaagcatt accatcagat gcggtgattc attttatagg atcattacaa
    12061 tctcgaaaag ttaaggacgt tataaacgac gtagattatt tccatgcttt agatcgattg
    12121 agcttagcca aagaaattaa caaacgtgca gaacataaaa ttaaatgttt cttgcaagtg
    12181 aacgtttcgg gagaagcttc taaacatggt attgctttag aagatgttga tcagtttata
    12241 gatgatctta aaaaatatga caaaatcgaa attgtaggtt taatgacgat ggcaccattg
    12301 acagatgatg aagcatatat tagatcgtta tttaaacagt tacgtttgaa aaaagaagaa
    12361 atacaacgac tcaatttaga atatgcgcct tgtgatgaat tatcaatggg aatgagtaat
    12421 gactatctta ttgcagttga agaaggtgcg acgtttgtta gaattgggac taaacttgta
    12481 ggagaagagg agtgagccac ttggctttaa aagatttatt tagtggattt tttgtaatag
    12541 atgatgaaga ggaagtagaa gtacctgaca aacaacaaca ggtcaatgaa gcgccagcaa
    12601 aagagcagtc acaacaaaca acaaaacaaa acgcaatcaa atcagtccct caaaaatctg
    12661 catcaagata tacaacaacg tcagaagaaa ggaataaccg tatgtctaat tattcaaaaa
    12721 ataattcacg taatgttgta actatgaaca atgctacacc aaacaatgca tcacaagaaa
    12781 gttcaaaaat gtgtttattc gaaccacgtg ttttttcaga tacacaagat attgctgatg
    12841 agcttaaaaa ccgccgtgcg acacttgtca atttacaacg tattgataaa gtatcagcga
    12901 aaagaattat tgatttttta agcggtactg tttatgcaat cggtggagat atccaacgtg
    12961 taggtactga tattttctta tgtacgcctg ataatgtgga agtagctgga agcattacag
    13021 accatattga aaatatggaa cattcattcg actaaggagt taacatatgg atataaatgt
    13081 gctagctaca atatttaaat ttatcctttt tgttgttgaa atttattatt tcggcatgat
    13141 tatatatttc tttacatctt gggtaccaag tattagagaa actaaggtag gttatttttt
    13201 agcgaaaata tatgaacctt tcttacaacc atttagaaaa gtaattccac ctattggaat
    13261 tatcgacata tcatcaatcg ctgcaattat cgttttagta ttattccaaa aagggttact
    13321 ccaaatcttt aattggattt taattcaatt acaataaatt aagcgacata acgaggcaag
    13381 ggtacatgat actttagcct cgtttttgat atgtattttt ctgaatataa gggcaataga
    13441 tggtatttta taattttttt aaggtagtga ttaacataga tatttatcaa cactttagac
    13501 aggaagaata cgaattaatt gatcagctaa cggataaatg tgatcaagcg gaacaacatt
    13561 atgcaccagt attaacgcat tttttagatc caagagggca atatatattg gaagtgattt
    13621 gtggcagtta tgaagattta aacgtctctt tttatggcgg gcctaatgct gaaagaaaaa
    13681 gagcaatcat ttcgccgaac tattatgaac ctaaagaaag tgactttgaa ttaactttaa
    13741 tggaaataga gtatcctgaa aaattcgtca ctttaaaaca tcaacatatt ttagggacat
    13801 taatgtcttt aggtatcgaa cgcgaacaag ttggagatat aattgtgaat gaacgaattc
    13861 aatttgtttt gacaagtaga ttggaatcat ttattatgtt agaattacaa cgtattaaag
    13921 gcgcatcagt taaactttat actattccag taacagatat gatacaatct aatgagaatt
    13981 ggaaaaatga aagtgcaaca gttagttctt taaggttaga tgttgttatt aaagaaatga
    14041 tacgtaaatc acgtacgatt gcgaaacaac taattgaaaa aaaacgtgtt aaagtgaatc
    14101 acactattgt tgattcagca gattttcaat tacaagcaaa tgatttaata tccatccaag
    14161 gttttggtag agcacacatt actgacttag gtggtaaaac taaaaaagat aaaacgcaca
    14221 ttacctatag aacattattc aaatagtaat gatttaagga ggataacaaa tgccttttac
    14281 accaaatgaa attaagaata aagagttttc acgtgtaaag aatggtttag aacctactga
    14341 agttgctaat tttttggagc aactaagcac tgaaattgaa cgtcttaaag aagataaaaa
    14401 acaacttgaa aaagtaatcg aagagagaga tactaatatt aagtcttatc aagacgtgca
    14461 tcaatctgta agtgatgctt tgatacaagc tcaaaaagct ggtgaagaaa ctaagcaagc
    14521 tgcagagaaa caagctgaag cgattatagc taaggcagaa gcgcaagcta atcaaatggt
    14581 tggtgacgcg gtagaaaaag cacgccgttt agcattccag actgaagata tgaaacgtca
    14641 atcaaaagta tttagatcgc gtttccgtat gttagttgaa gcgcaattag acttattaaa
    14701 aaacgaagat tgggattact tgttgaatta tgatttagac gctgaacaag tgacgcttga
    14761 aaatattcat catttgcatg aaaatgattt aaagccagat gaagttgcag caaatgcaca
    14821 aaataatgca tcaaatacac cagacaataa tcaacaatcc aatgattcag aaacaactaa
    14881 gaagtaagaa ttaaataaag acagacgcgt aatatacatt taacttttca cagcgaatta
    14941 ggtaatggtg agagcctagt aaaagcatgt atgttatatc actggctttt taatatttaa
    15001 ataatgtaat gagagaactc taagttgagt taataagggt ggtaccgcga gcaatcgtcc
    15061 cttttaattt aacttagagt tttttaaatt tttaaggagt gaaaaaaatg gattacaaag
    15121 aaacgttatt aatgcctaaa acagatttcc caatgcgagg tggtttacca aacaaggaac
    15181 cgcaaattca agaaaaatgg gatgcagaag atcaatacca taaagcgtta gaaaaaaata
    15241 aaggtaacga aacattcatt ttacatgatg gcccaccata cgcgaatggt aacttacata
    15301 tgggacatgc cttgaacaaa attttaaaag actttattgt acgttataaa actatgcaag
    15361 ggttctatgc accatacgta ccaggttggg atacacatgg tttaccaatt gaacaagcat
    15421 taacgaaaaa aggtgttgac cgaaagaaaa tgtcaacagc tgaattccgt gagaaatgta
    15481 aagaatttgc tttagaacaa attgaattac agaaaaaaga ttttagacgt ttaggtgttc
    15541 gtggtgactt taatgatcca tatattacat taaaacctga atacgaagct gcacaaattc
    15601 gtatttttgg agaaatggca gataaaggtt taatttataa aggtaaaaag ccagtttatt
    15661 ggtctccttc aagtgagtct tcattagcag aagcagaaat tgaatatcac gataaacgtt
    15721 cagcatcaat ttacgttgca tttgacgtta aagatgacaa aggtgtcgtt gatgcagatg
    15781 ctaaatttat tatctggaca acaacgccat ggacaattcc atcaaatgtt gcgattaccg
    15841 ttcatcctga attaaaatat ggtcaataca atgtaaatgg cgaaaaatat attattgcag
    15901 aagccttgtc tgacgctgta gcagaagcac tggattggga taaagcatca atcaaattag
    15961 aaaaagaata cacaggtaaa gaattagagt atgttgtagc acaacatcca ttcttagaca
    16021 gagaatcgtt agtgattaat ggtgatcatg ttactacaga tgctggtaca ggttgtgtac
    16081 atacagcacc aggtcacggg gaagatgact atattgttgg tcaaaaatat gaattgccag
    16141 taattagtcc aatcgatgat aaaggtgtat ttactgaaga aggcggccaa tttgaaggga
    16201 tgttctatga taaagctaat aaagccgtta ctgatttatt aacagaaaaa ggtgcactat
    16261 taaaattaga ctttattaca catagctatc cacacgactg gagaacaaaa aaacctgtaa
    16321 tcttccgtgc tacaccacaa tggtttgcct caatcagtaa agtaagacaa gatattttag
    16381 atgcaatcga aaatacaaac ttcaaagtaa attggggtaa aacacgtatt tacaatatgg
    16441 ttcgtgaccg tggcgaatgg gttatttctc gtcaacgtgt gtggggtgta ccgttaccag
    16501 tattttatgc tgaaaatggc gaaattatca tgacgaaaga aacagtgaat catgttgctg
    16561 atttatttgc agaacacggt tcaaatattt ggtttgaaag agaagcgaaa gacttactac
    16621 cagaaggatt tacacatcca ggcagcccta acggtacatt tactaaagaa acagacatta
    16681 tggacgtttg gtttgattct ggttcatcac accgtggcgt gttggaaaca agaccggaat
    16741 taagtttccc agcggatatg tatttagaag gtagtgacca atatcgtggt tggttcaact
    16801 cttctatcac aacttcagtt gctacaagag gagtatcacc ttataaattc ttactttctc
    16861 atggttttgt tatggacggt gaaggtaaga aaatgagtaa atctttaggt aatgtgattg
    16921 tacctgacca agtggttaaa caaaaaggtg ctgatattgc gagactttgg gtaagtagta
    16981 cggactattt agctgatgtt agaatttctg atgaaatttt aaaacaaaca tctgatgttt
    17041 atcgtaaaat cagaaataca ttaagattta tgttaggtaa cattaacgat ttcaatcctg
    17101 acacagatag cattcctgaa tcagagttat tagaagtgga tcgttacttg ctaaatcgtt
    17161 tacgtgaatt tactgcaagt acgattaaca actatgaaaa ctttgactac ttaaatattt
    17221 atcaagaagt tcaaaacttt atcaatgttg agttaagtaa tttctatttg gattacggta
    17281 aagatatttt atatattgaa caacgtgatt ctcatatccg tcgtagtatg caaacagtgt
    17341 tatatcaaat tttagttgat atgacgaagt tgttagcacc aatcttagtg catacagctg
    17401 aagaagtttg gtctcataca ccacatgtta aagaagaaag tgttcactta gcagacatgc
    17461 ctaaagttgt agaagtagat caagctttat tggataaatg gcgtacattt atgaatttac
    17521 gtgatgatgt gaaccgtgca ttagaaactg ctcgtaatga aaaagttatt ggtaaatcat
    17581 tagaagctaa agttacgatt gctagtaacg ataaatttaa tgcatctgaa ttcttaactt
    17641 catttgatgc attacatcaa ttatttatcg tgtcacaagt taaagttgta gataagttag
    17701 atgatcaggc aacagcttat gaacatggtg atattgtcat cgaacatgca gatggtgaaa
    17761 aatgtgaaag atgttggaac tattcagagg atcttggtgc tgttgatgaa ttgacgcatc
    17821 tatgtccacg atgccaacaa gttgtaaaat cactggtata attgaaattg tataaagtac
    17881 ttatacagat gatataaatt aaagctctct tcataatcat gttgtagttt ttgttgacat
    17941 gatgaagaga gtttttttgt gaataaaaaa atgaccaagt tactggttat atgtgttaaa
    18001 aaatgtgtaa tttactaaaa taaaaattat tcaggaatgg tacaaattct ctgaagctta
    18061 taaatgcgtt atagttgcta ttctcaatta tgttcgcgat aattttaagt aaaagtaagc
    18121 acagatattg aatttgatag gagttaattg aatgtatcat aacagtaacg caaactttgt
    18181 aaatggtatc actttaaatg tgagagataa gaatgaatta aagccatttt atgaggacgt
    18241 attaggatta aatattataa atgagacatt aacatcgata caatatgaag taggtcaaaa
    18301 taatcatgtc attacacttg ttgaattaca aaatggacgt gaacctttaa tgtccgaagc
    18361 gggactgttt catatcgcaa ttaaactacc tcaaattagt gatttagcta atttactaat
    18421 tcatttaagc gaatatgata ttccagttaa cggaggtata cagcctgctt cgttatcatt
    18481 attttttgaa gacccagaag gaaacggttt taaattttat gttgataaag acgaagcgca
    18541 atggacgagg caaaatgatt tagtaaaaat tgatattaga ccattaaatg taccgagatt
    18601 agtgagtcat gcaacaaaat tgttatggtt aggtattcca gatgacgcta ttataggtgc
    18661 attgcatatt aagacaattc atttatcaga ggtaaaagag tactacctcg attattttgg
    18721 attagagcaa tcggcatata tggatgatta ttcaatattt ttagcatcga atggctatta
    18781 tcaacatttg gccatgaatg attgggtatc agcaacgaaa cgtgtagaaa attttgatac
    18841 gtatggatta gcaattgttg actttcatta tcctgaaaca acacatttaa atttacaagg
    18901 tccggatggt atctattatc gctttaatca tatcgaagtt gaagattagt atatactttg
    18961 aatggacgaa ccatataatg aatcgttttt aatgatcttt ttatacaagt tatgaaggag
    19021 gctgggacat taagttctt
//
LOCUS       DAOBBH010000036        18957 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.036, whole genome shotgun sequence.
ACCESSION   DAOBBH010000036 DAOBBH010000000
VERSION     DAOBBH010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18957
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.036"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            372..1244
                     /locus_tag="P6L01_000909"
     CDS             372..1244
                     /locus_tag="P6L01_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDP3029994.1"
                     /translation="MSNVLTEIDSQYPYMTKNEKKIAKFILNSPQKVIKMRSQDLASL
                     LDISTSSVIRFSKKITDGGFHDLKINISKYVPKASSIYNVELMNNESTESLRTKLHTR
                     TTRALNHANNELNDKTIDQICHCLKRSETIFIYGFGASFVVATDLYQKLSRIGLNIQL
                     VQETHIFATLLATHNSNDSVILITNNGTQSEMQSMVKVIDDYHIPIITITSTRDNPVA
                     QASNIVLTYGKTDENEMHMGATTSLFAQMFTIDILYYRYVALNYHASLDFITQSKMAL
                     DNYRKHLSNINFKH"
     gene            complement(1631..3010)
                     /locus_tag="P6L01_000910"
     CDS             complement(1631..3010)
                     /locus_tag="P6L01_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDP3029995.1"
                     /translation="MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVI
                     LAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVG
                     MSEVIAVGEYMKFWFPELPTWIPGVIAILLLMAANLFSVKAFGEFEFWFALIKVVTII
                     LMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIG
                     ITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKI
                     GITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTV
                     VAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHP
                     EELEGHPFKMPGGAVTNYLTILFLILVLVGMVFNVETRISVLIGVIFLTIVTIYYFIR
                     YNKNNVKAK"
     gene            3290..3646
                     /locus_tag="P6L01_000911"
     CDS             3290..3646
                     /locus_tag="P6L01_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029996.1"
                     /translation="MLKQILPRATKISILFAVAFFIINYIGMEKPDILYLVGRTIIAT
                     LAFILICLTLFTIINSPERKIKLGTTLPIALIIGIIFGAIFLTVQIGVITGLIIGVIA
                     TFIWELIEKNKGGRSS"
     gene            3643..3828
                     /locus_tag="P6L01_000912"
     CDS             3643..3828
                     /locus_tag="P6L01_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029997.1"
                     /translation="MTTALIGVIVLILLIISFVPNYRAMKSAKEQGSNATRATIMVGI
                     DIILIVLIIVTLLLKYM"
     gene            complement(3924..4115)
                     /locus_tag="P6L01_000913"
     CDS             complement(3924..4115)
                     /locus_tag="P6L01_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3029998.1"
                     /translation="MMGYIILFFLAGPVILGVGNLVIGPIFNKQTPFRVQVRSFVVGS
                     MIYLILATIGYFLLLQGKL"
     gene            complement(4126..4761)
                     /locus_tag="P6L01_000914"
     CDS             complement(4126..4761)
                     /locus_tag="P6L01_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDP3029999.1"
                     /translation="MYRAVIFDFDGTIIDTEQHLFNVINKHLEMHNADPISIDFYRSS
                     IGGAATDLHDHLIKAIGSENKDKLYEEHHLTSTTLPMIDTIKSLMAFLKQRHIPMAIA
                     TSSVKAEIMPTFKALGLDDYIEVVVGREDVEQVKPDPELYLSAVQQLNYMPTQCLAIE
                     DSVNGATAAIAAGLDVIVNTNKMTSAQDFSNVDYVAKDIDYDQIVARFFTK"
     gene            complement(4836..5753)
                     /locus_tag="P6L01_000915"
     CDS             complement(4836..5753)
                     /locus_tag="P6L01_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HDP3030000.1"
                     /translation="MFQKLSIFATKSFLVWMLVAAVIGFIFPQHVATLGKWVPYLLGI
                     VMLGMGLTITPNDFKMVFKAPRAVIIGVCLQFSIMPTLAFIIAKSFHLPPDIAVGVIL
                     VGCCPGGTSSNVMSYLAKANVALSVSITTVSTLLAPFVTPALIYLFANEWLEVSFLSM
                     LWSVVQVVLIPIALGIVLQIINRKIAEKASTALPIISVVAISLILAIVVGGSKHQILT
                     TGLLIFLVVILHNVLGYTIGYWLARLLKLDRQDQKAVSIEVGMQNSGLAVSLAALHFN
                     PIAAVPGAVFSFIHNITGPILAKYWSKKL"
     gene            6021..6551
                     /locus_tag="P6L01_000916"
     CDS             6021..6551
                     /locus_tag="P6L01_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030001.1"
                     /translation="MLIDKFETYIINIAGLNDRTTRKKLSKLCKSVQFCDALQFSINK
                     QFNQYVLEISLPKQQLPYFISFLSFHQYSIFQVLSPKKINELLDSDNLYQSAKRFDIN
                     IDGLQDAFIKDKVIDIMNMFQNHTDITYTLNKSHAHIICTPEIFAKLLHTIATRNIDI
                     LSANYRSSSMSKARIS"
     gene            complement(6617..8221)
                     /locus_tag="P6L01_000917"
     CDS             complement(6617..8221)
                     /locus_tag="P6L01_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDP3030002.1"
                     /translation="MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQH
                     TFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINA
                     ILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRL
                     PEMVGVFQGLTFVVTISFFVMLPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYH
                     FLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSTKPLKEQFPYGFML
                     QGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPY
                     LFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVIIGLIF
                     VAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDSVDSKRASTGNEYED
                     KAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVADTEYFTHQQMAHGLVKSGKSIQ
                     VVVGMTVPQVREAFEQMVEDQSSEDK"
     gene            8551..9315
                     /locus_tag="P6L01_000918"
     CDS             8551..9315
                     /locus_tag="P6L01_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDP3030003.1"
                     /translation="MFLDEHINRNFDKLNDNDLHIAHFINTHIDECKNMKIQDLAQFT
                     HASNATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERV
                     DYQLLTEKMHTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKS
                     EPNDLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTF
                     YLTDGTDYSSFISYHIFFETLVRKYNEYLLHNDISN"
     gene            complement(9373..9879)
                     /locus_tag="P6L01_000919"
     CDS             complement(9373..9879)
                     /locus_tag="P6L01_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HDP3030004.1"
                     /translation="MAKFNVENEHVEVEIEKLYKFSPELVYEAWTKKDLLKQWFMTSA
                     RTNKEIEADVKEGGKYRIVDQQRNGKVNVIEGIYKSLVMDEYVKMTIGMPGLSETQDV
                     IEVEFFERETGGTQMLFYYRSLVEKERRFTNLEYKQKKKEYHDAMVHGFELMFDKMYH
                     VIETSTQQ"
     gene            complement(10046..11359)
                     /locus_tag="P6L01_000920"
     CDS             complement(10046..11359)
                     /locus_tag="P6L01_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDP3030005.1"
                     /translation="MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITIT
                     VIVALLMNRKESFAKKVEVFTKGAGHSNIILMMLIFILAGAFSNTAEKMGGVKSTVNL
                     GLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
                     GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
                     NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDA
                     VSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSV
                     ADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAA
                     GVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH"
     regulatory      complement(11436..11531)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11584..12153)
                     /locus_tag="P6L01_000921"
     CDS             complement(11584..12153)
                     /locus_tag="P6L01_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030006.1"
                     /translation="MALIVSRAILFLILALAFGIATVFPEQKLPLYIVFTLFVAVAII
                     MNVWFTHQNVKKHIIKFNKYVAYFLSFVSGLLYIGFVIYTLTIGSYDVLPIIIFAISI
                     VLVQILENILGISIKNTRLSNKQLKVISKQRVQQFKVISLILVSVSIVFIFIALMLKW
                     WLLLVISIIVLIIITVAMIVYEKYYTTPD"
     gene            complement(12640..13521)
                     /locus_tag="P6L01_000922"
     CDS             complement(12640..13521)
                     /locus_tag="P6L01_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDP3030007.1"
                     /translation="MATQDPRTKFKTTEYEKQEQEVPGLQSEMTPAPDCGETSYQGHQ
                     RLQGYKMLVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAV
                     LIPGDIRDEQFNYDLVEQAYQQLGGLDNVTLVAGHQQYHDDIHGFTTEAFTETFETNV
                     YPLFWTVQKALEYLKPGASITTTSSVQGYNPSPILHDYAASKAAIISLTKSFSEELGP
                     KGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEES
                     SYTTGQVFGVSGGVQID"
     gene            complement(13631..14752)
                     /locus_tag="P6L01_000923"
     CDS             complement(13631..14752)
                     /locus_tag="P6L01_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HDP3030008.1"
                     /translation="MGLKDQAVTWRRYFHQFPELSDKEFKTTQKIKDILTEHHIRILD
                     LPLMTGLVAEVGQGLSCIAVRADIDALPIQELVEQDFKSENEGVMHACGHDIHMASIL
                     ATAVKLKEIEGTLTGRVKFIFQPAEELGHGAFKIIETDALKDVQAVLGFHNDPSRSVG
                     TFAIKTGAITSAVDRFEFHIKGVGGHAAKPEQCNDPVIVLAQLINSIQSIVSRNLSAF
                     DEAVVTIGQISCGNTWNVIADHAYVQGTVRSFDPVVRKLVETRLQDIADGLAQAYNMK
                     INLNYTHLPGAVMNDEALTHKAIAVAQHVGYKVEMMEQPLTIGEDFSGYSQHFPSVFA
                     LIGSHSEYDLHHPQYKPDERILEKVPEYFVEFVKRLLHE"
     gene            complement(15028..16266)
                     /locus_tag="P6L01_000924"
     CDS             complement(15028..16266)
                     /locus_tag="P6L01_000924"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HDP3030009.1"
                     /translation="MNDLIINHIAELILPKSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGQHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAI
                     AALKLKLSPSEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKDGKVIVDNTLSFKA"
     gene            complement(16266..17927)
                     /gene="hutU"
                     /locus_tag="P6L01_000925"
     CDS             complement(16266..17927)
                     /gene="hutU"
                     /locus_tag="P6L01_000925"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HDP3030010.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLA
                     EEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            18087..>18957
                     /locus_tag="P6L01_000926"
     CDS             18087..>18957
                     /locus_tag="P6L01_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDP3030011.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGL"
BASE COUNT         6338 a         3729 c         2637 g         6253 t
ORIGIN      
        1 aaaagtttct tcaaattgta aagatgcatt aatcgagtta taatcataat gattaagatg
       61 gtagatttag tcttatataa ctacatttac taagatttaa atagatgagt acataatgta
      121 taaattggga atatacagta tgacttacgg taatatatca atatattgat atattatttg
      181 cacatgggag tatgctattt tttatctata catcttaaaa aaatttacag ttttacaatt
      241 caatatcatt aaacaaacaa catattcagc ttatttttca ttctcttacg ttacaagttg
      301 taaataccat cgcgttaaac aacaatagcc aacgctaata aaatatattg cgttaaagtg
      361 gagtgattaa tatgtcaaac gtactaacag aaatagatag tcaatatcca tatatgacta
      421 aaaatgaaaa aaagatagct aaatttatac taaattcacc tcaaaaggtg attaaaatga
      481 ggtctcaaga cttagcaagc ttattagata ttagtacatc atcagtcatt cgattcagca
      541 agaaaattac tgatggcggt tttcacgact tgaaaataaa tatatctaaa tatgttccta
      601 aagcatcatc aatttacaat gttgaattaa tgaataacga aagcacagaa tctttaagaa
      661 ctaaactcca tacacgtact acacgcgcac ttaatcatgc aaataacgaa ttaaatgata
      721 aaactattga tcaaatatgt cattgtttaa aacgttctga gacgattttt atatatggtt
      781 ttggcgcatc ttttgtagtt gctaccgact tataccaaaa gttatcaaga ataggtctta
      841 atattcaact tgttcaagaa acacatattt ttgctacatt attagcaact cacaattcga
      901 acgacagtgt tattcttatt actaacaatg gtacgcaaag tgaaatgcaa tcaatggtta
      961 aagttattga tgactaccat atacctataa ttacaattac tagtacaagg gataatcccg
     1021 tagcacaggc atcaaatatt gttttaactt atgggaaaac tgatgaaaat gagatgcata
     1081 tgggagcaac aacttcatta tttgctcaaa tgttcactat tgatatttta tattatcgtt
     1141 atgttgcatt aaattatcac gcatcattag atttcataac acaatctaaa atggctttag
     1201 ataactatcg taaacattta tcaaatatta actttaaaca ttaacagtct tatataaatt
     1261 ctttaatcat gtcatataat aatttatgca tcattttcaa agcttacatg tcttttatct
     1321 tgtataacat acgcatatga ccaatgattt gataaggcca ttcaatttct tatattaaaa
     1381 acaaacgcag ttgattaacg gattcattcc atatatcaat tgcgtttgtt attttaataa
     1441 tgctattttt tattttaaag cccacagtaa aaactaacga taatattgcc acttataagt
     1501 ttttcacact acaacttcga tttaagctat atttataaaa aaatgcgcct actcataaca
     1561 ttctttggct atttaacttt aataagctag ggccaaattt gtcataaaat aagcgcacat
     1621 tataatcata ctattttgct tttacattat ttttattata tctaataaag taataaatcg
     1681 ttacaatcgt taagaagatg acaccaatta gcactgatat tctagtttca acattaaata
     1741 ccataccaac caatactaat attaagaata agattgttaa atagttagtt actgcgccac
     1801 caggcatttt gaatgggtga ccttccaatt cttcaggatg taattttcta aaacgtaagt
     1861 gactgaataa aatcataaac caaggaatca tacctggtaa aatagacgca ctataaacgt
     1921 atacgaaaat actcttcgct ccatcgatat ataaaggtaa aattacgttt aataaagcac
     1981 caattaaaat accaagagat actgctacaa cagtgtacaa tggtacacca tttctcatga
     2041 ttttagtaaa tatttttggc atttcacctt tatgagcaag tgtataaatc atacgactcg
     2101 cactgaaaat acctgagtta catcctgaca ttgctgcagt taatacaaca aagttaatta
     2161 atccagctgc aaatgtaata ccgattttag caaatgttgc aacaaacgga cttccaatgt
     2221 ctcctaattg attccaagga tacactgaaa caataacaaa aatagcgcct aaatagaaaa
     2281 ttaaaattct ccagataaca ccattcactg ctttaacgat atttttctga ggatctttag
     2341 tttcacctgc cgtaatacct atcagttcca caccttggta tgaaccaatt acaattgata
     2401 atgcaaagaa gaaaccaaca ataccatttg gcataaatcc gccatttgtc catagattag
     2461 aaatacctac cgcatggcca ccatttccaa aaccaaagaa aataagacca aaaccagcaa
     2521 taatcattaa aataattgtt acaactttaa ttaaagcaaa ccaaaattca aattctccaa
     2581 acgcttttac cgagaataaa ttcgctgcca ttaataataa aatagcaata acaccaggaa
     2641 tccaagttgg caattccggg aaccaaaact tcatatattc tcctactgcg atgacctcac
     2701 tcataccaac tacaatccat tggaatatat tactccatgc tgtcatataa cctgctgcag
     2761 gatgtatata atcacttgca aatgttgcaa atgatcctgt tgtagggttt aaataaatca
     2821 tttcccccat tgctctcatg attaaaaata aaaagatacc cgcaattaaa tatgcaagga
     2881 taactgatgg gcctgtccat ttaattgtac ttgtggcacc catgaaaagc cccacgccaa
     2941 tcgtaccacc aagtgcaatc atttgaattt gacgggcact caatcccctt tgtagttcat
     3001 tgttctccat aacttcttac cccatctctg attgatacta aataaatcca taaaaaaact
     3061 tattgccgta attatactat aaattatcga ttttggtaca ttaatatttg cataccatgc
     3121 aatcgtttac agaactacta tagcaggctt ttgcaacttt gcaaacaatt attttgtgat
     3181 ttttctaaaa aattaaaaaa gttgctcaag taccgttgag tcacatacaa cttcagctat
     3241 tctcggtaat tatgatataa ttttaataat attaaaaagg agtatatcaa tgttaaaaca
     3301 aatactgcca cgtgctacaa aaatatcgat actctttgct gtagcatttt tcatcataaa
     3361 ttatatcggt atggaaaaac ctgacatcct atatttagtt ggaagaacta ttattgcaac
     3421 acttgctttc atactcattt gtcttacatt atttactatt attaattctc cagagcgcaa
     3481 gattaaactc ggtacgactt taccgattgc attaattatt ggtatcattt tcggcgccat
     3541 atttttaacg gtacaaatcg gtgtcatcac tgggttaatc atcggtgtga ttgctacgtt
     3601 tatttgggaa ttgattgaaa aaaataaagg aggtcgttca tcatgacaac agcattaatt
     3661 ggtgtaattg tattaatctt actaattatt agctttgtgc caaactaccg tgctatgaaa
     3721 tcagcaaaag aacaaggaag taatgcaaca agagctacaa ttatggtagg catagatatt
     3781 atcttaattg tacttatcat cgtgacatta ttattaaaat atatgtaact atttaaacca
     3841 ccctatctca tacataaaca tgaacgttct gccatttcac atacatatgg tcgtccattt
     3901 aatgaggtaa ggtggttttc tcgttaaagt ttaccttgta atagtaaaaa atagccaatt
     3961 gttgcgagta ttaagtaaat cattgaacca acaacaaaag atcttacttg cacgcgaaat
     4021 ggtgtctgtt tgttaaatat aggaccaatc accaaatttc caacgcctaa aattactgga
     4081 ccagctagaa aaaacaatat aatgtaaccc atcatgatac gcctcctatt tcgtaaagaa
     4141 acgcgctaca atttgatcgt aatcaatatc ttttgctaca taatctacat tagaaaagtc
     4201 ctgtgcgctt gtcattttat tcgtattaac aataacatct aatccagctg caatcgcggc
     4261 tgttgcacca tttacagaat cttcaatagc caaacattgt gtcggcatat aatttaattg
     4321 ttgtactgca gataaatata attcagggtc aggtttaact tgttcaacat cttctctacc
     4381 aacaactacc tctatataat cgtctagacc taatgcttta aaggtgggca ttatttccgc
     4441 tttcacacta cttgtggcaa ttgccatagg aatgtgacgt tgctttaaaa atgccatcaa
     4501 tgatttaatc gtatcaatca tcggtaatgt tgtactagta agatgatgtt cttcataaag
     4561 tttatcttta ttttccgaac caatcgcttt aattaaatgg tcatgcaaat ctgtagctgc
     4621 tcctccaata gaagaacgat aaaaatcaat gcttatagga tcggcattat gcatctctaa
     4681 atgtttatta ataacattaa ataaatgttg ttccgtatct attattgttc catcgaaatc
     4741 aaatataact gctctataca tatatttact cctctatttt ttctctttat caaagcttaa
     4801 catagtccga tgaccacttc attctattag tgcaattata acttttttga ccaatacttt
     4861 gctaaaatag gccctgttat attatgaatg aaactaaaca ctgcgcctgg tactgctgca
     4921 attggattaa aatgcaatgc tgctaatgac acagctaaac cagagttctg cattccaact
     4981 tcaatactga ctgctttttg atcttgtcga tctaatttta aaagacgagc taaccaatat
     5041 ccaatcgtat accctaatac gttatgtaaa ataactacta aaaatattaa taatcctgta
     5101 gttaagattt ggtgcttact gccacctaca actattgcta aaattaatga aatagcaaca
     5161 actgatataa tcggcaaagc tgtagaagct ttttcagcaa ttttacgatt aataatttgc
     5221 aaaacaatac ctaaagcaat tggaattaat acaacttgaa caactgacca caacatactc
     5281 aggaaagata cttccaacca ttcatttgca aatagatata ttaacgcagg tgtaacgaat
     5341 ggcgctagca acgtagagac cgtcgtaata gaaacagaaa gtgctacgtt agctttggct
     5401 aaataactca ttacattact tgatgtccca cccggacaac atccaactaa tattacgcca
     5461 acagcaatat caggtggtaa atgaaaagac tttgcaatta taaatgctaa tgtgggcata
     5521 atactgaatt gtagacagac accaataatt actgctctag gtgctttaaa gaccattttg
     5581 aaatcattag gtgtaattgt taatcccata cctaacataa caataccaag taaataaggt
     5641 acccacttac ctaatgtcgc aacatgttgt gggaaaataa atccaataac agctgctact
     5701 aacatccata ccaaaaaact cttcgtggca aatatactca atttttgaaa cattgttaac
     5761 acatcctcgt cgataatatt gaaaaatatt accataagac cagtaaggtt acaattactt
     5821 ttctgaaaat taagattatt tagccattct cttcttattg caaatatttt atcactgata
     5881 aacaattatt ttaaacttca ttcaaaagtc ttagtgaata aagaaaaaaa gcgtggtaaa
     5941 atcaacttga acaaaattaa gtcaccttta taaatattaa acgtgtattt aatgccatgt
     6001 attcctcggg aggtcctcac atgttaattg ataaattcga aacatacatc atcaacatcg
     6061 ccggtttaaa tgaccgtact actagaaaaa aattaagtaa gttatgcaaa agtgtacaat
     6121 tttgtgatgc gttgcaattc tcaataaaca aacagtttaa tcagtatgta ttagaaatta
     6181 gcttaccaaa gcaacaactt ccatacttca tttcattttt aagttttcat cagtatagta
     6241 ttttccaagt actttcacca aagaaaataa atgaattact tgattctgat aatttatatc
     6301 aatctgcaaa gcgtttcgat atcaatattg atggcttgca agatgctttc attaaagaca
     6361 aagtcatcga tattatgaat atgttccaaa atcacactga tatcacttat acgttgaata
     6421 agtctcatgc acatattata tgtacgccag aaatatttgc taaattgtta catacgattg
     6481 caactagaaa tatcgacatt ctatctgcca attatagatc gtcatctatg agcaaagctc
     6541 gtatatcata ataaaagaca catctatacg atgatcattt acaatgattg cgtatagtga
     6601 tgtgtctttt tttgtattat ttatcttcgg acgattgatc ctcaaccatt tgttcaaatg
     6661 cttcacgaac ttgtggtact gtcataccta ctacaacttg gatactctta ccactcttta
     6721 ctaatccgtg tgccatttgt tgatgcgtga aatattccgt atccgcaact ttactttcat
     6781 cataaacagt taagcgtaat cttgttgtac agttagtaac atctttgata ttttctttgc
     6841 cgcctaaacc gtcgaggtaa tacgcagctt tgtcttcgta ttcattacct gttgaagcac
     6901 gtttagaatc tacgctatca ccttttttat ttttgtaatc ttgcttagaa aataatttaa
     6961 cttcctcttc tgttttctta cgtccaggta atggaatatc aaatttcaaa attaagaatc
     7021 tgaataagaa gaagtaaatc gctacaaata tcagaccgat aataacttgt gcaacataag
     7081 taccccaatg ttctttacct aatggcaacc agtttgttga aataaagtct aacaagccac
     7141 cacccatgtt acctacaaca ccaaatgcgt acattaaagt atccattgat gctgctaaaa
     7201 ctgcgtgtaa aacaaataag tatggtgcga tgaataagaa tgtaaattct aatggctcag
     7261 taatacctac aacgactgct gttaatgttg caggaataag taatgctgct acttttttac
     7321 gattttcttt aggtgtcgta gcatacattg ctaaagcaat acctaaacat ccaaatactt
     7381 tcccatttcc ttgaagcata aagccataag ggaattgctc ttttaatggc ttagtactct
     7441 tagcaaactc atttaaatgt tgtaaccatt cagctttcaa gccatgattg actactactg
     7501 gtccaacttc aattggtgca taaataaagt gatgtaatcc tgttggtatt aatacacgtt
     7561 ctaagaagtg gtacagccaa acaccgatat agcctgaagc gatgataaag tgttgcattg
     7621 aaccaatacc gtgctgaaca gttggccaaa taacacaagt aattgctgct aatggtaaca
     7681 tcacaaaaaa tgaaattgta accacaaatg ttaaaccttg gaatacacca accatttctg
     7741 gtaatcgttt actgtagtat ctattgtgaa tccacgtaat aatagctgaa ataataatac
     7801 cacctagaat attcgtatcc aatgtggcaa tacctgcaat tgattttaaa ccaggtacat
     7861 tttcaacgcc tttttctaaa ttagcgccaa atgtatgtgg ccattgcgtt aaaatggcat
     7921 ttataaatgt attaaacatt aagtaaccca tcaatgctgc aagcgctgca tgacctggtg
     7981 cttttttagc taaagaaagt ggtaaaccta caacaaatac aacttccata tgcgtaaaaa
     8041 taacccagcc accagattct atcactgacc aaaatttaaa ccaaaacgta tgttgatctg
     8101 ctaaactccc catgattgta ggatttttaa ataatgtcgc aaagccaagc actatcccaa
     8161 agaaagcgaa cattaatacc ggtacaatca ttgcactacc gaaacgcttt atcgcattca
     8221 tcttctattc cctccatatc atctttccta acaatacatc taattagatt catttataaa
     8281 tagatgtctt actatttaaa tataatatat agtaaacgct tacacaccta caacgacatt
     8341 gacgtatttt gaaagtattt tgtataatca gattatcttt tcatatagtg aaaatttttt
     8401 cacgacctta tatatgacat cgttgtattt gtaatacatt cgttttaaac gcataatcaa
     8461 acctatatca atacacaaat atatataatg acatacaaga ttttaatgta ataacgatct
     8521 atcacacatt tattttcaag gaggttgaat atgtttttag atgaacacat taatcgaaac
     8581 tttgataaac ttaatgataa tgatttacat atcgctcact ttatcaatac acatatagat
     8641 gaatgtaaaa atatgaaaat acaagattta gcgcaattca cacatgcctc gaatgcaacc
     8701 attcacagat ttacacgcaa attaggtttt gacggttata gtgattttaa atcgtacctt
     8761 aaatttgaaa gtaataagac acaccaatta ccttctaact ctatcgatag tttcaaacaa
     8821 gaaatcgata gtacattcag ttatttagaa cgcgttgatt accagcttct tactgaaaaa
     8881 atgcatacag cttcaacaat atatttgtat ggtacagggc gtgcacagtt aaacgttgct
     8941 tcagaggcac agcgtatatt gttaacgtta cataatcatg tcattgtact tcatgatgaa
     9001 catgaactta aaatgatttt gaacaagagt gaacctaatg atttattctt tattatttca
     9061 ttatcaggtg aaacacatga attattagaa gtaacaaact tgcttcaact tagacaaaaa
     9121 tactttattt ctgttactac aatgaaagat aatacccttg ctcaaaaagc aaattacaac
     9181 gtctatgtat ccagtaacac attctactta actgatggta cagactattc tagcttcatc
     9241 agttatcata tattctttga aacacttgta agaaaatata atgagtattt actacataat
     9301 gatatttcaa attagaaaaa aggaagttat gatttaatgg ccttgagcct atcataactt
     9361 ccttttatca ttttattgtt gtgttgatgt ttcgataacg tggtacatct tatcaaacat
     9421 caattcgaaa ccatgcacca tggcatcatg atattctttt ttcttttgct tgtattctaa
     9481 attagtaaat cgtctttctt tttcaactaa tgaacgataa taaaatagca tttgggtgcc
     9541 acctgtttca cgttcaaaaa attctacctc aatgacatct tgtgtttcac ttagtccagg
     9601 cataccgata gtcatcttaa cgtattcatc cataactaaa gatttataaa tgccttcaat
     9661 cacatttact ttgccattac gttgttgatc tacaatacga tatttaccgc cttctttaac
     9721 gtccgcttca atctctttat tcgttctggc tgatgtcata aaccattgtt tcaacaaatc
     9781 tttctttgtc caagcttcgt atactaactc tggagaaaat ttataaagct tttcaatttc
     9841 aacttcgaca tgttcattct ctacattaaa ttttgccact gttgtccacc cactttcgct
     9901 cttactttta ttttaacgta tttttgctca gttccaaaca tagatgatca tcatttttaa
     9961 aagattagcg ttatacggtg agtacaacat gatctgttaa tataacaagc caccttactt
    10021 ggctacatcg atatattgtt aagcattaat gtttcatttc ttgactagtg ttctttttta
    10081 gctttggaaa attaaataaa atcgcaataa gtccgcatac acctaataat ataggataaa
    10141 tgctgtatgg gaataacatt aatggtgaaa taccagctac accagccgct gaaatgactt
    10201 gcgggctata tggtaataaa ccttggaagc agcctccaaa tatatcaaga atacttgctg
    10261 atttccttga atctacatca tattcatctg caatattttt agctaaagga cctgacataa
    10321 taatagagat ggtgttgttt gccgtggcaa tatctgcgac acttaccaaa ctcgcaattc
    10381 ctaattctgc gccacgcttt gatttcactt tagagcgaac aaattgcaac aaccattcaa
    10441 taccaccatt gtgttgaata ataccgacta aaccaccaat tagcaacgca atcatagcaa
    10501 tatcttccat gcttataata cctttggaca ctgcatctag tagtcccatc caaccgaatg
    10561 aaccatctat gagaccaatg attccagcta ataatgttcc gccaatcaat acgataatga
    10621 catttacacc taataatgct aataccaata ctaaaatata cggtacaact ttaattagat
    10681 tataatcata gtttttagca tgatttaaag aaatgccatt cgttaagaaa tacagaataa
    10741 taatcgttaa aatagcacct ggcaatacaa ttttaaagtt tactctgaat ttatctttca
    10801 ttttcgtatg ttgtgttcta accgcagcaa ttgttgtatc tgaaatcatt gatagattat
    10861 cgccgaacat tgcacctcca acaactgtag ccattgctag cgcagttggt acatctgtcg
    10921 cttgtgcaaa tccgtaacca acaggtgcta ttgcagcaac agttcctaca gacgtcccca
    10981 tagatataga aacaaacata caaatcacaa acaatcctac aataattaaa ttttctggga
    11041 ttaatgatag tcctaaatta actgtcgact ttacgccacc cattttttca gctgtatttg
    11101 aaaatgcacc tgctaaaata aaaatcaaca tcattaaaat aatgtttgaa tggcctgcac
    11161 ctttcgtgaa gacctcaact tttttagcaa atgattcttt tcgattcatt aataacgcca
    11221 caattaccgt tatcgtaatt gcaacattta atggcattga agtaaaatca cctgtgataa
    11281 tacctacgcc taaaaacaac gccacaaata ataacaaggg gaataatgcc caagcattgc
    11341 tctttttatg tacttccatc ctttttacct gctttccaat taaaaatacc tctttctcac
    11401 aaacgatgaa gaaagaggtt ttcatgtgct ttacctgctt atcttcaaac cattacggtt
    11461 actggaattg gcacattcga gatgttgccg aggcttcata gggccagtcc ctccacctct
    11521 ctagataagt gatgcttatt tacgtttacg ttacaagata atccttagta cgtcaatcat
    11581 aaattaatca ggagtcgtat aatatttttc ataaacaatc attgctactg taataataat
    11641 caaaacaata atgctaataa caagtaaaag ccaccattta agcattaatg caataaaaat
    11701 gaacacgata gacacactta ctaatattaa tgatatgact ttaaattgct gaacacgttg
    11761 cttggagatg actttcaact gtttgtttga tagacgcgta ttttttatac tgattcccag
    11821 tatattttct aatatttgaa ccaatacgat acttattgca aatataataa ttggtaaaac
    11881 atcatagctc cctatagtta atgtataaat tacaaatcca atgtaaagta accctgagac
    11941 aaaggataaa aagtatgcga cgtatttgtt aaacttaatg atatgctttt taacgttttg
    12001 atgggtaaac catacattca taataatcgc aactgctaca aataatgtga atactatata
    12061 taatggtaat ttttgttcag gaaaaacagt cgctattcca aaagctaatg ctaaaatcaa
    12121 aaataatata gctctagata ctattaatgc cataataaca acccctttgt ttaatatcga
    12181 gtttgcaaat ttacgtttat cagcatttct atgatcagta cttctacggg tagcgtttct
    12241 atgtaattta catcatctta acatataaat acttcgctat ttaattgaaa acatatctta
    12301 ttattctttg tccgttctga catttaatat ctaaccttag gcatttcact tgttaataaa
    12361 tttaactttc ttccactaac cgtccctaaa cccaatcccg caacagtttt taactttttc
    12421 gttgttgtcc tgacatcctc attaagaaag tttattctgc ttaaaactta taatccacac
    12481 cctgagcaaa cgctccttat gacagagtat taaaataagc cgataaagat acacaccttt
    12541 accgactatt taaaatacac ttcaccaatt cattttaatt taatggattg aagtaactaa
    12601 attaatatta tgctgttcaa ttaaaagctt catacaaccc taatctattt gcactccacc
    12661 gctaacaccg aacacttgtc cagttgtata acttgattcc tctgacgcta atagcacata
    12721 agttccacat aactcaacag gttgacctgc acgacctaaa ggtgtttttt gaccaaatgt
    12781 tgggatttta ctttgaggtt gcccaccaga aatttgtaat ggtgaccaga atggaccagg
    12841 tgctacacag ttcactctaa ttccttttgg tcctaattct tctgaaaaac ttttagttaa
    12901 tgaaataatt gctgcttttg aagcggcata atcatgaaga ataggactag gattataacc
    12961 ttgtacagat gatgtcgttg taattgacgc accgggtttt aaatattcca atgctttttg
    13021 aactgtccaa aatagcggat agacattcgt ttcaaatgtt tctgtaaatg cctcagttgt
    13081 aaatccatga atatcatcat gatactgttg atgtccagca actaaagtaa cattatctaa
    13141 gccacctaat tgttgatatg cttgttcaac aaggtcatag ttgaactgct catctcttat
    13201 atcaccagga attaacactg ccttttgacc actttcttca attacttggc gtacttcttg
    13261 tgcatcttgt tcttcactcg gaagatagtt aatcgctaca tctgcacctt ctttagcata
    13321 cgcaattgct gctgcacgcc ctattgctga gtcaccacct gtgactaaca ttttatagcc
    13381 ttgtaagcgt tgatgacctt ggtaagacgt ttcgccacaa tcaggtgctg gcgtcatttc
    13441 agattgtaaa cccggtacct cttgttcttg tttttcatat tccgttgttt taaattttgt
    13501 tctaggatct tgagttgcca ttttttaaca tctccttatt cgcttaatgg ttattattta
    13561 cccaatcttc ctaggaactt aatcatgatt acactaaaat tactttcttc tttataaaaa
    13621 caagctcgaa ttactcatgc aatagtctct ttacaaattc aacaaaatac tcaggtactt
    13681 tttccagaat cctttcatcc ggtttatatt gagggtgatg taaatcatat tcactatgag
    13741 aaccaattaa cgcaaataca cttggaaaat gttgactata acctgaaaaa tcttctccaa
    13801 tcgtaagcgg ctgttccatc atttccacct tatatccaac atgttgggct actgcaattg
    13861 ctttatgcgt caatgcctca tcattcatca cagcgccagg taaatgtgta taatttaaat
    13921 taattttcat attatatgct tgagccaatc cgtccgcaat atcttgtaat cgtgtttcta
    13981 caagctttcg taccacagga tcaaaactac gcactgtgcc ttgtacatac gcatgatcag
    14041 caatgacatt ccaagtatta ccacatgata tttgtccaat tgttactacc gcttcatcaa
    14101 acgcagatag atttctacta actatggatt gaatactatt aatcaattgc gccaacacaa
    14161 taactggatc gttgcattgt tctggctttg cagcatgacc acccacgcct ttaatatgaa
    14221 actcaaagcg atctactgct gatgtaattg cccctgtttt gattgcaaat gtacctaccg
    14281 aacgcgatgg gtcattatga aaacccaata ctgcttgtac atcttttaat gcatctgttt
    14341 caataatttt aaaagcgcca tgtcctagtt cttctgctgg ttgaaaaatg aatttaacac
    14401 gcccagtaag agtgccctca atttctttta attttacagc cgtagccaaa atactagcca
    14461 tgtgaatatc atgaccgcac gcatgcataa caccttcatt ttcagattta aaatcctgtt
    14521 caacaagctc ttgaatcggt aaagcatcaa tgtcagcccg aacagctata caactcagac
    14581 cttgtcccac ttcagcaacc agcccagtca taagcggtaa gtctaatatt ctaatatgat
    14641 gttctgttaa aatatcttta attttttgtg tagtcttaaa ttctttatcg gatagttctg
    14701 gaaattgatg aaaatacctt ctccaggtaa cagcttgatc ttttaatccc atcggtcatt
    14761 ccccttcctt aagtcaatga tatgttgtct accctacgat gatcatcttt gactattaaa
    14821 cgatgatttc acaacaatgt actcttgtta attactttcg ctaatgatag acagttgttt
    14881 aataatatcg taacactgtt gtcaaactat tctaactttt ataattaaga atctatacaa
    14941 aaacgtgttc tcggataaac ttgtttttac aaaccacaaa agctctaaac attagtgtaa
    15001 accaatgctt agagctttct aattatttta tgctttaaaa gataatgtgt tatctacgat
    15061 gaccttaccg tctttaataa ctttttctgc gtgattgata ccaaaatgat atggaatata
    15121 ttcatgattt ggtgcatccc aaattactaa attagcctta tcacctgtgt taattgtacc
    15181 cgcgttaata tctattgctt tagcagcgtt gaccgtaaca gcattccaca cttcactagg
    15241 cgaaagcttt aattttaatg ctgcaatggc cataacaagt tgcaagttat tcgttacact
    15301 gctgcctgga ttataatcag tagctaacgc gatggcacca ttattatcaa gcatacctct
    15361 cgcatctgca taatcttctt tacctaaata gaacgtcgtt gcaggtaaga ggacagctac
    15421 agtatcacta tttcgcaact tttcttttcc tttatcacta gaagctacta agtggtctgc
    15481 tgatattgct tgttcatcaa ttgctaattc cagtccgcct aacggatcaa tttcatccgc
    15541 atgtattttc actttaaaac ctgcttcttt ggctttttgc atataatgtt gcgattgttc
    15601 tattgtaaat acacctgttt cacagaaaat atccgcaaag tctgcatatt gttttacttc
    15661 cggaagtaac gcaatcattt cttctaaaaa tgcctcattt gaacttgcct ctttaggtac
    15721 agcatgaggc cctaggaaag tatgtttcat gtctaaatca tatttctcag ctaaacgatt
    15781 agacactttc aattgcttca gttcattttc tctatctaat ccataaccac tcttactttc
    15841 aactgcaagc acgccgtgtt taatcatagt aagcaaatca tgctctgctt ttttaaacaa
    15901 gtcatcttcg gatgtttctc tagtagcatt aacggtagat aatatgccac cacccatttc
    15961 taatatttca aggtaagact taccttgacg ttttaatgac atctcatgtt ctcgagatcc
    16021 accaaatgtt aaatgggtat gagcatctac taatgccgga gaaactactt ttccactagc
    16081 atctatcgtc tcagtcgcat catagtcatc tgtatgttgt ccagcatata caattttgcc
    16141 atctttaatg acaactgtac catttttcac aacatttaat tcatctaatt ccttaccctt
    16201 caaaggttta tctgttgatt tcggtaaaat taattctgct atgtgattaa ttattaaatc
    16261 attcattatt tatcacctgc tttatcaatc attggaatat gaataccttt ttctttagct
    16321 gtttgaatag cgatgtcata gccagcatca acatgtcggg caacacccat acctggatca
    16381 gtcgtcaata cacgttccaa tcttctttca gcacgctctg atccatctgc tacaacaacc
    16441 atacccgcat gaagtgaata tcccatgcca acaccgccac cgtgatggaa tgaaatccat
    16501 gaaccacctg cagctgtgtt aatgagtgca ttcaatacag cccaatcacc aaccgcgtca
    16561 ctgccatctt tcatactttc tgtttcacgg ttaggactag caactgaacc agcatctaaa
    16621 tggtctcgtc caataacaat tggtgctgaa atttcaccgt cacgtacaag acgatttaaa
    16681 gctaagccca ttttcgctct ttctccatag cctaaccaag caatacgtga tggtagtcct
    16741 tgatatgaaa ttttttcttc agctaaatca agccatctta ataacttttc attttctggg
    16801 aaaagtttgc gcatttcttc atccgcacgc tcgatatctt ttggatcacc actcaacgca
    16861 gcaaagcgga atggcccttt accttcacag aataatggtc taatgtaagc tggtacaaag
    16921 cctgggaagt caaaagcatt tttcactccg ttattgaagg ctacttgacg aatattgtta
    16981 ccataatcaa atgctacagc gccacgtttt tggaattcaa gcattaattc aacatgcttt
    17041 gccattgaag cttgtgacag ttcaacatat tttttcggat ctttttcacg caatactttc
    17101 gcttcttcta cagagtatcc ttgtggcaca tatccattta gcggatcatg tgcacttgtt
    17161 tggtcagtaa taatgtcaat tttaaatcct ttttctagaa tcgcttgatg gatgtctaca
    17221 gcatttccaa ctaacccgat tgataatcct tctccacgtt ctttcgcctc ttctgctaat
    17281 tttaatgctt catctaaatc agctgtttta acatcacagt atttcgtatc aattcgctta
    17341 tcaacacgtg tttcatcaac atccacgcaa attgctaccc catgattcat agtaattgct
    17401 aacggttgcg caccacccat accacctaaa cctgctgtca gtgtaacagt gcctgctaaa
    17461 tctccattaa agtgttgatt acctagctcg gcaaatgtct cataagtacc ttgcacaata
    17521 ccttgagaac caatatatat ccaactaccg gctgtcatct gtccatacat gattaaacct
    17581 tttttatcta attcattaaa atgatcccag tttgcccatt caggcactaa tactgaattt
    17641 gaaattaata cacgtggcgc ttcttcatgt gttttaaata cagcaactgg ctttcctgat
    17701 tgtactaaca ttgtctcatc tgattctaat tctcgtaacg ttttctctat tgcttcaaaa
    17761 gcttcccaat tacgtgctgc ttttccaata ccaccataaa caactaaatc ttctggtctt
    17821 tcagcaactt ctgggtctaa attgttgtat aacattctaa gtactgcttc ttgttcccaa
    17881 cctttacact caatactcaa accttttttt gcttgaattt ttctcataaa attcgctcct
    17941 gttcttttaa gaagttaatt ccactaaatt taaaacgctt acattattat cttcaatatt
    18001 cattatagta tgttaaaata tagccaacaa atataaataa actaattatc catagcttga
    18061 acctataaat aaaaggagca aaacacatga aaattattca gttagaatac ttcttggcta
    18121 tcgtgaaata taatagtttt actaaagctg cacaattttt acatattagc cagccatctt
    18181 taactgctac gattaaaaaa atggaagcag atttaggtta tgacttattt acacgttcaa
    18241 caaaagacat caagattacc gaaaaaggaa tacagtttta tcgttatgcg agcgaattag
    18301 ttcaacaata tcgatccacg atggaaaaaa tgtatgattt aagcgttaca tcagaaccaa
    18361 ggataaaaat tgggactctt gaatctacga atcaatggat tgcgaattta attcgaaagc
    18421 accattccga ctaccctgaa cagcaatatc gtttatatga aatacatgac aaacatcaat
    18481 ctatagagca attactgaat tttaatattc atttagctat aacaaatgaa aaaataaccc
    18541 acgaagatat aagatccatt cctttatatg aggaatctta cattttatta gcacccaagg
    18601 aaacatttaa aaatcaaaat tgggtagatg ttgaaaattt gccactcata ttaccaaaca
    18661 aaaattctca agtgcgcaaa cacttagatg actattttaa tagaagaaat attcgtccaa
    18721 atgtcgttgt agaaacagat cgattcgaat cagcagttgg atttgttcat ctcggcttag
    18781 gttacgctat cattccgaga ttttattacc aatcatttca cacgtctaat ttagaatata
    18841 aaaaaattcg tccaaactta ggccgaaaaa tttatatcaa ttaccataaa aaacgcaaac
    18901 actccgaaca agtacataca ttcgtacaac aatgccaaga ttatttatat ggacttt
//
LOCUS       DAOBBH010000037        17837 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.037, whole genome shotgun sequence.
ACCESSION   DAOBBH010000037 DAOBBH010000000
VERSION     DAOBBH010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17837
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.037"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(38..637)
                     /locus_tag="P6L01_000927"
     CDS             complement(38..637)
                     /locus_tag="P6L01_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDP3030012.1"
                     /translation="MAFELPKLPYAFDALEPHFDKETMEIHHDRHHNTYVTKLNAAVE
                     GTDLESKSIEEIVANLDSVPANIQTAVRNNGGGHLNHSLFWELLSPNSEEKGTVVEKI
                     KEQWGSLEEFKKEFADKAAARFGSGWAWLVVNNGQLEIVTTPNQDNPLTEGKTPILGL
                     DVWEHAYYLKYQNKRPDYIGAFWNVVNWEKVDELYNATK"
     gene            complement(913..1323)
                     /locus_tag="P6L01_000928"
     CDS             complement(913..1323)
                     /locus_tag="P6L01_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDP3030013.1"
                     /translation="MNTNDAIKILKENGLKYTDKRKDMLDIFVEEDKYINAKYIQQVM
                     DENYPGISFDTIYRNLHLFKDLGIIENTELDGEMKFRIACTNHHHHHFICEKCGDTKV
                     IDYCPIDQIKLSLPGVNIHKHKLEVYGVCESCQD"
     gene            complement(1310..2173)
                     /locus_tag="P6L01_000929"
     CDS             complement(1310..2173)
                     /locus_tag="P6L01_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDP3030014.1"
                     /translation="MIDALLNFDFMRYSLISGILIGFIAPLIGAFIVVRRLSLIADAL
                     SHVTLGGISFGMFLLTIMPTLVFINPMWFGILFAIVGALLIEKLRTSYTAYQEIAIPI
                     IMSAGIALSAIFISLADGFNQEIVGLLFGSISAVNISDLTTIIVIAIIVVLFITLFYK
                     ELFILSFDEEYSKVIGIHKWIQFLFIVIVAMVISASMRVVGILLVSALITLPIAISMR
                     ITKSFKQLILLSVFLGELSVILGLVLAFYMDISPGGVIVVLLVILLMITMAYQKMRMK
                     FKKGANINEYK"
     gene            complement(2215..3000)
                     /locus_tag="P6L01_000930"
     CDS             complement(2215..3000)
                     /locus_tag="P6L01_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDP3030015.1"
                     /translation="MRGENTMTPVFELKNVNYYYDHKKVLENINIKINKGEFLAIVGP
                     NGAGKSTLLKLILGLLPLQSGEIFVEGIDFKNKKTSIKLSYVSQKANAFNSGFPASVK
                     EVVLSGLTKTKRLFQTFNSKDNEKVIKVLERLNISDLIHKNIAELSGGQQQRVMIARA
                     LISEPAVLVLDEPTNGIDAKHVSEFYNTLDQLKQEGITIILVTHDIGVVADTATEVAC
                     LNKHLHFHGTTDEFKSLDEVEISKIYGHPVRFVDHQHNRECCN"
     gene            complement(3126..4016)
                     /locus_tag="P6L01_000931"
     CDS             complement(3126..4016)
                     /locus_tag="P6L01_000931"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDP3030016.1"
                     /translation="MLLGSHVSMSGKKMLEGSAIEAYEYGETTFMIYTGAPQNTRRKS
                     IEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIG
                     AKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
                     ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSK
                     NDRGAQKDRHENIGFGYIGFDALNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLE
                     IEMLKQQQFDPELKNKVMQQ"
     gene            complement(4026..5372)
                     /locus_tag="P6L01_000932"
     CDS             complement(4026..5372)
                     /locus_tag="P6L01_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDP3030017.1"
                     /translation="MAKHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNL
                     IGQSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKAGV
                     SVKVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAKTGHLHVHLASYLVIDEADLMI
                     DLGLIEDVDYIAARLEDNANIAVFSATIPQQLQPFLNKYLSHPEYVAVDSKKQNKKNI
                     EFFLIPTKGAAKVEKTLNLIDILNPYLCIIFCNSRDNANDLARSLNEAGIKVGMIHGG
                     LTPRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRT
                     GRGNYKGVAITLYSPDEEHNISLIEDRGFVFNTVDIKDGELKEVKAHNQRQARMRKDD
                     HLTNQVKNKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG"
     gene            complement(5486..6586)
                     /locus_tag="P6L01_000933"
     CDS             complement(5486..6586)
                     /locus_tag="P6L01_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDP3030018.1"
                     /translation="MKIADLMTLLDHHVPFSTAESWDNVGLLIGDGDVEVTGVLTALD
                     CTLEVVNEAIEKGYNTIISHHPLIFKGVTSLKANGYGLIIRKLIQHDINLIAMHTNLD
                     VNPYGVNMMLAKAMGLKNISIINNQQDVYYKVQTYIPKDNVGPFKDKLSENGLAQEGN
                     YEYCFFESEGRGQFKPVGEANPTIGQIDKIEDVDEVKIEFMIDAYQKSRAEQLIKQYH
                     PYETPVFDFIEIKQTSLYGLGVMAEVDNQMTLEDFAADIKSKLNIPSVRFVGESNQKI
                     KRIAIIGGSGIGYEYQAVQQGADVFVTGDIKHHDALDAKIHGVNLIDINHYSEYVMKE
                     GLKTLLMNWFNIEKINIDVEASTINTDPFQYI"
     gene            complement(6589..7266)
                     /locus_tag="P6L01_000934"
     CDS             complement(6589..7266)
                     /locus_tag="P6L01_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDP3030019.1"
                     /translation="MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCECGI
                     AGEVIQGPFQAAVKNVATNHLVERIDVRLGDGLSVIHPEDVIDNITICGMGGPLIAKI
                     LKDGQDKLSQHPRLILQSNIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEHST
                     QLIELSSDELKFGPKLLNSKNEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEI
                     AVIERVL"
     gene            complement(7397..8503)
                     /gene="rpoD"
                     /locus_tag="P6L01_000935"
     CDS             complement(7397..8503)
                     /gene="rpoD"
                     /locus_tag="P6L01_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDP3030020.1"
                     /translation="MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEK
                     LQNFDIDSDQMDDFFDQLNDNDISLVNEKDSSDTDEKLNPSDLSAPPGVKINDPVRMY
                     LKEIGRVNLLSAQEEIELAKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDL
                     IQEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LIRVQRQLLQDLGRDPAPEEIGEEMDLPAEKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGK
                     VFGVTRERIRQIEAKALRKLRHPSRSKRLKDFMD"
     gene            complement(8727..10544)
                     /locus_tag="P6L01_000936"
     CDS             complement(8727..10544)
                     /locus_tag="P6L01_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDP3030021.1"
                     /translation="MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEK
                     TPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQS
                     NSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
                     APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVG
                     YSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDT
                     AGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATLKTGQHLLQQGLNVF
                     VIQLPSGMDPDEYIGKYGNDAFTTFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKE
                     LSHDISLMKSSILQQKAINDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYDEYG
                     GYIEPEPIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVF
                     EVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYVNVINEK
                     GQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM"
     gene            complement(10605..11423)
                     /locus_tag="P6L01_000937"
     CDS             complement(10605..11423)
                     /locus_tag="P6L01_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDP3030022.1"
                     /translation="MEKIKIIVASDSIGETAELVARAGISQFNPKQCKNELLRYPYIE
                     SFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASV
                     EEKPYNEPGIVHRLDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKTPL
                     SQYLAHKSYKVMNVPIVPEVTPPDGLYDIDPKKCIALKISEEKLNRIRKERLKQLGLG
                     DTARYATEARIQEELNYFEEIVSEIGCPVIDVSQKAIEETANDIIHYIEQNKSK"
     gene            complement(11434..12057)
                     /locus_tag="P6L01_000938"
     CDS             complement(11434..12057)
                     /locus_tag="P6L01_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDP3030023.1"
                     /translation="MELSQRQERIIEIVKTKGPITGEQIADKLNLTRATLRPDLAILT
                     MSGFLEARPRVGYYYSGKSKGKFFNEKLRQFEVKDYMSQPVVLRENTTVYDAICTIFL
                     EDVSTLFIINEDNDFVGVCSRKDLLRASMIGADIHTVPISVNMTRMPNVTYLEESELV
                     IYAADRMIEKEIDSIPIVRKKDNQKYEVIGRISKTTIAKLLVALYKE"
     gene            12392..13783
                     /locus_tag="P6L01_000939"
     CDS             12392..13783
                     /locus_tag="P6L01_000939"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HDP3030024.1"
                     /translation="MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNN
                     VKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKL
                     IEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQG
                     VTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRT
                     REFEQMELEFFCKPGEEIEWQNYWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNA
                     TTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLG
                     ADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQ
                     LSSKFSIDFDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPI
                     SELEAFLTEKTKF"
     gene            complement(13933..14679)
                     /gene="recO"
                     /locus_tag="P6L01_000940"
     CDS             complement(13933..14679)
                     /gene="recO"
                     /locus_tag="P6L01_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDP3030025.1"
                     /translation="MRQKGIIIKAVDYGESDKIITILNEHGAKVPLMARRAKKVKTGL
                     QAQTQLFVYGLFIYNQWRGMGTLNSVDVISQHYKLQMDLYVSSYASLAAETIERSMDE
                     GDIAPYNYQLLQFVLEKIESGTSAQLMSVVVMLKCMKRFGFTASFNRCAVSGNDTQAD
                     LIGYSFKFDGAISRQEASKDVHAVILSNKTLYLLDVLQKLPIDKMNSLNIHQEIIDEM
                     SDIILMLYREYAGMFFKSQKLINQLKRLEQ"
     gene            complement(14707..15606)
                     /gene="era"
                     /locus_tag="P6L01_000941"
     CDS             complement(14707..15606)
                     /gene="era"
                     /locus_tag="P6L01_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDP3030026.1"
                     /translation="MTEHKSGFVSIIGRPNVGKSTFVNRVIGHKIAIMSDKAQTTRNK
                     IQGVMTRDDAQIIFIDTPGIHKPKHKLGDYMMKVAKNTLSEIDAIMFMVNANEEIGRG
                     DEYIIEMLKNVKTPVFLVLNKIDLVHPDELMPKIEEYQSYMDFTEIVPISALEGLNVD
                     HFIDVLKTYLPEGPKYYPDDQISDHPEQFVVGEIIREKILHLTSEEIPHAIGVNVDRM
                     VKESEDRVHIEATIYVERDSQKGIVIGKGGKKLKEVGKRARRDIEMLLGSKVYLELWV
                     KVQRDWRNKVNFIRQIGYVEDQD"
     gene            complement(15607..16011)
                     /gene="cdd"
                     /locus_tag="P6L01_000942"
     CDS             complement(15607..16011)
                     /gene="cdd"
                     /locus_tag="P6L01_000942"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDP3030027.1"
                     /translation="MSYQPHYFQEVRKAQQESYSPYSQFKVGAYLKTKDGRTFYGTNV
                     ENASYPLSICAERASLVSAISQGYRPGDFESITVTVDADKPSSPCGACRQVLKELCDD
                     DMPVYMTNHKGDMVMMTVAELLPFGFSGKDLE"
     gene            complement(16022..16366)
                     /locus_tag="P6L01_000943"
     CDS             complement(16022..16366)
                     /locus_tag="P6L01_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDP3030028.1"
                     /translation="MKRFKYALDGLKILIQKDYKFLLHVFAMIVAIVFGLVLNINRIE
                     WIFILIAIALVLTVEALNTAIEYVVDLVTVEYHDLAKYAKDIAAFSVLIVSILAFIIG
                     LIVFLPHFIALF"
     gene            complement(16369..16836)
                     /gene="ybeY"
                     /locus_tag="P6L01_000944"
     CDS             complement(16369..16836)
                     /gene="ybeY"
                     /locus_tag="P6L01_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDP3030029.1"
                     /translation="MFTIDFSDHTGLVKDAWYKQIEDLLEFAKKEEHIEDDAELSVTF
                     VDKQEIQEINRTYRDKDKVTDVISFALEEDEPEIDFSGLDIPRVLGDIIICTDVAQEQ
                     ANNYGHSFERELGFLALHGFLHLLGYDHMTEADEKEMFGRQDTILNAYGLTRD"
     gene            complement(16837..17784)
                     /locus_tag="P6L01_000945"
     CDS             complement(16837..17784)
                     /locus_tag="P6L01_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDP3030030.1"
                     /translation="MPGIIQIDDMNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAV
                     KGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITK
                     DAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGAVKRIV
                     LTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLA
                     YMRGRTLEDAFVILDEAQNTTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLK
                     EAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN"
BASE COUNT         5609 a         3317 c         2411 g         6500 t
ORIGIN      
        1 acggtctcat ttaagagacc gaacaagtaa tcatacttta ttttgttgca ttatataatt
       61 cgtcaacttt ttcccagtta actacattcc aaaatgcgcc aatgtagtca gggcgtttgt
      121 tttgatattt taggtaataa gcgtgttccc atacgtctaa acctaaaata ggtgttttac
      181 cctcagttaa tggattatct tggtttggtg tagtcacaat ttctaactgg ccattgttta
      241 cgactaacca agcccaacct gaaccaaagc gtgcagctgc tttgtcagca aattcttttt
      301 taaattcttc taaagaaccc cattgttctt taattttttc tactacagta cctttttctt
      361 ctgagtttgg tgaaagtaac tcccagaata atgaatggtt taaatgtcca ccgccattat
      421 tacgtacagc agtttggatg ttagctggta cactgtctaa attagcaaca atttcttcaa
      481 tagatttaga ttctaaatct gtaccttcta ctgcagcatt taatttcgta acatacgtgt
      541 tatgatgtct gtcatggtga atttccatag tttctttgtc aaaatgtggt tctaatgcat
      601 caaatgcgta tggtaatttt ggtaattcaa aagccataaa taatcatcct cctaaaatgt
      661 ctgtaagtaa ataataacaa gcagtgactg gttcaacaaa gaatttgctt aaattctact
      721 acttattatt ttctctactc atttaatata actcaaatca aaaataatta aacattttgt
      781 atataaaaag ttaacagatt tgccataaaa tcatacgaac ggagtatgaa atgaaccttt
      841 atcttctata atttaaaaaa tgagatttat gcatacatct gaccaaatgt gcataaatct
      901 catttcttta tattaatctt ggcaagactc acatacacca taaacttcaa gtttgtgttt
      961 gtgaatatta acaccaggta gtgataactt tatctgatct attggacaat aatctattac
     1021 ctttgtatct ccacactttt cacagataaa atgatgatga tgatggtttg tacaagcgat
     1081 tctaaacttc atttcaccat caagttctgt attttcaata atccctaaat ctttaaataa
     1141 gtgcaggttt ctatatattg tgtcgaatga aattccagga taattttcat ccataacttg
     1201 ttgtatatac tttgcgttta tatacttatc ttcttcgaca aaaatatcta acatatcttt
     1261 acgtttatct gtatatttta aaccgttctc ttttaaaatt ttaatagcat catttgtatt
     1321 cattgatatt agctcccttt ttaaacttca ttcgcatttt ctgataagcc attgtaatca
     1381 taagtaaaat aactagtaga actacaatta caccacccgg agaaatgtcc atatagaaag
     1441 ctaggactaa gcctaatatt actgataatt cacctaaaaa tacacttagt aatatcaatt
     1501 gcttaaaact ttttgttatt cgcatactta ttgcaattgg taacgtgatt aaagcactta
     1561 ctaacagtat ccctacaaca cgcattgagg cagaaataac catcgctaca ataacaataa
     1621 ataaaaattg aatccattta tgaatgccaa tgactttact atattcctca tcaaatgaca
     1681 atataaataa ttctttataa aacaatgtaa taaacagaac aactatgatg gcaatgacaa
     1741 taatcgttgt taaatcactt atattcactg cgcttattga gccaaatagc aatccaacaa
     1801 tttcttgatt gaacccatca gctaatgaaa tgaagattgc actcaaggcg ataccagcac
     1861 tcattataat tggaatagca atttcttggt aagcagtgta tgacgttctt aatttttcaa
     1921 ttagaagcgc acctactatt gcgaataaga ttccaaacca cattggatta ataaatacta
     1981 gtgttggcat aatagtaagt aaaaacatac cgaaggatat accacctaaa gttacatgac
     2041 ttagagcatc agctataagt gatagtcgtc taacaacgat aaaagcaccg attagaggcg
     2101 caataaaacc tatcaagata ccactaatta aagagtacct cataaaatca aaattcaata
     2161 atgcatctat caattgtgac acgcctttcc attttaaata aactcaaatc tttattaatt
     2221 acaacattct cgattatgct gatgatcgac aaaacgtaca ggatgtccat aaatttttga
     2281 aatttcaact tcatcaagtg atttaaactc atcagttgta ccatggaaat gcaaatgctt
     2341 atttaaacat gctacttcag tagcagtatc tgctacaaca ccgatatcat gagtaactaa
     2401 gataatggtg ataccttctt gttttaattg atctaaagta ttataaaatt cacttacatg
     2461 ttttgcatca ataccattcg ttggttcatc aagtactaat actgcaggtt ctgaaatcaa
     2521 tgctcgggca atcattacac gttgttgttg accacctgat aattctgcta tatttttatg
     2581 aattaaatca cttatattca gtctttctag tactttaatc actttttcat tatctttgct
     2641 attaaatgtt tggaaaagac gttttgtctt tgttaatccg cttaaaacaa cttctttaac
     2701 acttgctggg aaacctgaat taaaggcatt tgctttttgt gatacatagc ttaatttaat
     2761 tgatgttttc ttatttttaa aatcaatacc ttcaacaaaa atctcaccac tttgtaaagg
     2821 taataaccct agaatcaact tcaataatgt tgatttacca gcaccatttg gtccaacaat
     2881 cgctaaaaat tcacctttat ttattttaat gtttatattt tctaacactt ttttatgatc
     2941 atagtagtaa ttgacatttt tcaattcaaa gactggtgtc atcgtattct cacctcgcat
     3001 tcaactatac aactcctagt aacatatgta aacagtaatg tttacgactc aaaattagac
     3061 aaaataaaga gatatgcccc cttcaagttt tatttatcgc atttcttgaa gagagcatta
     3121 tcattttatt gttgcataac cttatttttt aattctgggt caaattgctg ttgttttaac
     3181 atttcaattt caagtttata tggcggtttt ttatttttct tatcttcacc aacataaggt
     3241 gtttctaaga ttttcggaat atctttaaaa ctatcatgat gcacaatgta atttaatgca
     3301 tcaaaaccaa tgtaaccgaa gccaatattt tcatgtcggt ctttttgagc gccacggtca
     3361 tttttagaat cattgacatg aacaactttg attctgtcga ctccaataat tttatcaaat
     3421 tcatttaata cgccatcaaa gtcctctttc acattatatc cagcatcatg cgtatgacat
     3481 gtatcaaaac atactgataa acgttcgtta ttatgaactc catcaataat acgtgctaac
     3541 tcttcaaacg agcgaccaat ctctgtacct ttacctgcca tcgtttcaag cgcaatacgt
     3601 acattattgt cattcgttaa aacttcattt aatccttcaa taatcttatt aattccggca
     3661 tcaacaccag ctccaacatg cgcacctgga tgtaatacaa tatctttagc gcctatagct
     3721 tgcgttcttt caatttcttg ttgcaagaaa tctacaccaa gattaaacgt ttctggtttg
     3781 gttgtatttg caatattaat gatgtatggt gcatgaacaa caatattaga taagccatat
     3841 ttttccatca cttcatgacc tttagttata tttaaatctt caatactttt acggcgcgtg
     3901 ttttgaggtg caccagtata aatcataaat gttgtttcac catattcata cgcttctata
     3961 gcagaacctt ctaacatctt tttaccactc attgaaacat gtgatcctaa taacataaaa
     4021 cacacctaac cttttttgtt ttgcttacgt ttttgtctat tttgctgctt actaaattgt
     4081 ttacgctctt gacgtttcat tttttcaact tcttgtttaa atttcttctt ataacctggt
     4141 ttaactttgt ttttaatttt acttcgaact ttgttcttca cttgattagt taaatggtca
     4201 tctttgcgca ttcttgcttg acgctgattg tgcgctttaa cttcttttaa ctcaccatct
     4261 ttaatatcaa cagtattgaa tacaaaaccg cgatcttcta ttaatgaaat attgtgttct
     4321 tcatcaggac tataaagcgt aattgctaca cctttataat tcccacgacc agttcgtcca
     4381 actctatgcg taaagaagtc aatatcattt ggcacatcaa aattgatgac atgactaaca
     4441 ccttcaatat caataccacg agatgctaaa tcgctggcaa taacgtattg gaattctaaa
     4501 ttacgtatac gtttcatttg ttgtttacgt tcacgtggcg ttaaaccacc atgaatcata
     4561 ccaactttaa taccagcttc atttagtgaa cgtgctaaat catttgcatt atctctacta
     4621 ttacagaaaa taatacataa gtatggattt agtatatcaa ttaaatttaa agttttttca
     4681 actttagctg cacctttagt aggtattaaa aagaattcga tgttcttttt attttgtttt
     4741 ttactgtcga cagctacata ttctggatga cttaaatatt tatttaaaaa tggttgtaac
     4801 tgttgtggaa ttgtagcact aaacaccgca atatttgcat tatcttccaa tcttgcagca
     4861 atgtaatcta catcttcaat taatcctaag tcaatcataa gatccgcttc atcaataact
     4921 aaatatgatg ctaagtgcac atgtaaatgt cccgttttag ctaagtcatt aattctagta
     4981 ggggtgccta taatcaattg tggttgtgca ttacaacgtt gtctatcttt ctctatatct
     5041 gtaccaccaa taaaaacttt aactgaaaca ccagctttaa attggcttaa atggttcgct
     5101 gcatcgtata gttgttgtgc aagttctctt gttggtgcaa ctacgattgc ttgtggttct
     5161 tttatttcac tatcaattaa ctgcattaat ggtaataaaa atgcatgaga tttccctgta
     5221 cccgtttgag attgaccaat taaatttgtt ctctttagta ttcttggaat aattcgattc
     5281 tgaatttcag ttggtttttc aaaattaagg tctttcacag cgtcaattaa actagattct
     5341 agattaaatt gttcgaatgg atgttttgcc atgatttggc ctccttatat gttcatcttt
     5401 actattataa agtataaatg tgctatttta aataaaaata ttaaaacctc gaaatttcaa
     5461 aatttagaaa tcccgagatt ttagtttaaa tatattgaaa tggatctgta ttaattgtag
     5521 atgcttcaac atctatattt attttttcta tattgaacca attcattagt aacgttttta
     5581 aaccttcttt catcacatat tcgctgtaat gattaatatc aattaaattc acaccatgaa
     5641 ttttagcatc taaggcatca tgatgtttaa tatcacctgt aacaaagaca tctgcgcctt
     5701 gttggacagc ttgatattca tatccaatac ctgaaccacc aataattgca atacgtttaa
     5761 ttttctgatt agactcacca acaaaacgga cacttgggat atttaattta gatttaatat
     5821 cagctgcgaa atcttccaat gtcatttgat tatccacttc tgccataacg ccaagtccat
     5881 aaagggatgt ttgttttatc tcaataaaat caaataccgg tgtttcatat ggatggtatt
     5941 gtttaattaa ttgctcagcc cttgactttt gatatgcatc tatcataaat tcaattttaa
     6001 cttcatctac atcttcaatt ttatcaattt gtcctattgt tggattagct tcaccaactg
     6061 gtttgaattg ccctcttcct tcactttcaa agaaacaata ttcataatta ccttcttgcg
     6121 ctaatccatt ttcactaagc ttatctttaa atggtccaac attatcctta ggtatatatg
     6181 tttgaacttt atagtataca tcttgttgat tatttattat tgaaatgttc ttcaaaccca
     6241 tcgccttcgc caacatcata ttgacaccat acggatttac atctaaattt gtatgcatcg
     6301 ctattaaatt aatgtcatgt tgaattagtt ttctaatgat caaaccataa ccattagctt
     6361 ttaatgatgt tacgccttta aagattagag gatgatgact aataatagta ttataacctt
     6421 tttcgattgc ttcatttact acttccaacg tacagtctaa tgctgttaaa acaccagtaa
     6481 cttcaacatc tccatcacct attaacaatc ctacattatc ccaagattca gcagtactaa
     6541 atggcacatg atgatctaac aatgtcatta aatcagctat tttcattact ataacaccct
     6601 ttcaattaca gcaatttcgt cattaatttg agctaaacgt tgatgatgtt gttcagtatt
     6661 gagtttcgat ttaatatgat aaagtgcttc taactctctt tgccattttt ttataaaata
     6721 ttcatttttg ctgttcagca attttggtcc gaatttcaat tcatcagatg atagctctat
     6781 taattgtgta gaatgttctg ctacaacaat ttcataaata tggccttttt cttccattat
     6841 tatttcatca attatttcat aattcaattg ttgtaatgtt tgtcttaaat tttcagtttg
     6901 tatattactt tgtaaaatca accttggatg ttgacttaac ttatcttgcc catcttttaa
     6961 aattttagca ataagtggtc cgcccatacc acaaattgtg atattatcga ttacgtcctc
     7021 aggatgaata acacttaagc catcccctaa acgtacatca attctttcta ctaaatgatt
     7081 tgtagctaca tttttcacag cagcttgaaa agggccttga ataacttctc cagcaatacc
     7141 gcattcgcat aaatggtttt gaattgcata gattggcaaa taagcatgat ctgagccaat
     7201 atccgcgatt gtaccttgtt ttaaaaatcg acttaccgtc gttaatcggt tatttaacga
     7261 aatcatttta ttctccttta tgaaaaaaga ggttgaacat tattcaatgt accaacctca
     7321 ctcaagttaa atcttaatct aaaaataaat aagcgcttat tcatgtcttg gtatcaatca
     7381 ttaaaattga tataaattaa tccataaagt ctttcaaacg tttactacga cttggatgtc
     7441 ttaattttct aagtgctttt gcttcaattt gtcgaatacg ttcacgtgta acaccgaaaa
     7501 ctttaccaac ttcttcaagt gttcttgttc tgccgtcatc aagaccaaat cttaatcgta
     7561 atacattttc ttctctatca gttaatgtat caagcacatc ttctaattgc tcttttaata
     7621 attcataagc agcatgatct gaaggacttt gtgcttcctg atcctcaata aagtctccta
     7681 aatgactatc atcttcttca ccaattggtg tttctaatga aacaggttct tgcgcaattt
     7741 ttaaaatttc acgaactttt tctgctggta aatccatttc ttcaccaatt tcttctggtg
     7801 ctggatctcg acctaagtcc tgtaataatt gacgttgaac acgaattaat ttattaattg
     7861 tttctaccat atgcacaggg atacgaatcg tacgtgcttg gtcagcaatt gcacgagtga
     7921 ttgcttgtct aatccaccat gttgcatatg ttgaaaactt aaatcctttg ttaaagtcaa
     7981 atttttcaac agctttaata agacccatat taccttcttg gattaaatca aggaataaca
     8041 taccacgacc tacgtatctt ttagcaatac ttacaactaa acgtaagttc gcttctgcaa
     8101 gtcttgattt tgctacttca tcaccttgtt caatacgttt ggctaattcg atttcttctt
     8161 gtgcacttaa taagttaaca cgcccaattt ctttaaggta catacgaact gggtcattta
     8221 ttttaacacc tggaggggca ctaagatcac ttggattcag tttctcgtca gtatctgaac
     8281 tatctttttc attaactagt gaaatatcat tatcatttaa ttgatcaaag aaatcatcca
     8341 tttgatcaga gtcgatatca aaattctgaa gtttttcagc aatttcttca tgacttaaat
     8401 gaccctcttt tttacctttt tcaattaatt gcttcttaac atcttctaat gttaatgtcg
     8461 gatcaattgt ttgtttttta attttaactg tgttatcaga catgaaacgg cctcccgatt
     8521 ttaaatatga acattcgaaa tttattcaat attgctattt taaactaaat tcttaattaa
     8581 ttcatccata tttttaattt tattttacaa attggaacta atcccaatat ttatttttca
     8641 atagtgttgt ttaaatgcgg taactatcat ttacccttat acattttgac ataatcatta
     8701 ttcgtattat tctttaaaat cacatgctac atgcgttctt tattcttagc aacaatttgc
     8761 tgtaaatagt atttttgtaa ttctacatcg ccaatccttg tagcttccct taatttatga
     8821 ttcaatgact caattgtttc ttgtcctttt tcattaataa cattgacata atcatcaatt
     8881 tcattttcat atggttcgtc attcaaatta tattgttcta agctaattag tgtttctctc
     8941 aactcatttg aattaacata ctgcacagca tcactgatat tatattgatc attttccgca
     9001 taaaaatcat gtaagacttc gaatacatat ttaaaatgct gatttgtgaa gttatcctta
     9061 tcaacacttt cataataatt taaaaatgta tctttatctc tcattaaatg ttttaaaaat
     9121 gctcgctccg ctttttcttg acggctcaaa ttgtcaaatt gtgccatacc aattggctca
     9181 ggttcaatat aaccgccata ctcatcataa ccgccatact catcttctgg ataataattg
     9241 gctggtgctt gattgaattg tatttcgtta gctaactgct caggactaac attgaaaaat
     9301 ggcgcaacat catttatagc cttttgttgc agaattgatg acttcataag tgaaatgtca
     9361 tgactcagtt ctttcaaata acgttcatat gaaaggtcat tatgtgcaat ttcatctttt
     9421 aatatactta ctttataatg tgcaaatgac tttttgtcat tttttacaaa agtagtaaat
     9481 gcgtcgttgc catacttacc aatgtattca tccggatcca tgccagatgg caattgtata
     9541 acaaatacat ttagcccttg ctgtaacaaa tgttgacctg ttttaagtgt tgcttcacta
     9601 cccgcaaaat ccccatcaaa cattaatgtt atatttgatg ttaactttcg tataaaggta
     9661 atatgttcat ctgacaactg tgtacccatt gttgcaacaa cgtttttcaa gccagcagta
     9721 tcagatttta taacatccat aaaaccttct agtaatacaa tttcatctaa ttttctaatt
     9781 gatttacgtg ctttatctaa gttatataac aactttcttt tttgaaagat aggcgtttca
     9841 ggactattta ggtattttgg ttcttgaccg gtatatgttc gacctgaata tccaacaatt
     9901 cttccttgcg catttttcaa aggaaacata atacgatttc gaaatctatc gtaataactg
     9961 aaattttctt cgttacgtga taataatccg gcttcatatg ctaattcaat atcgtaaccc
    10021 tttttttgaa gaaaatcatg acaaaaatgt gagctatcgg gtgcaaagcc aatgcctcgc
    10081 tctttaataa gcgcatctgt aaaaccacgt tcttgtaagt atgttaatgc ttgttcgcct
    10141 tcgactgtct ttgttaaagc gtaataataa aattcttgta ttaactcatg catttcaatc
    10201 atttgtaaat catcagaagc aatttgaaca tttgagttag attgtgttgc ctcaatatct
    10261 acagcaacat taactctatc acctaattct ttaaccgctt caacaaatga tatgtcttta
    10321 atttcttgag taaattgaaa aacattgcca ccttttttac aaccaaaaca atgacagatt
    10381 tgtttatctt cagaaactgt aaatgaaggt gtcttttcat catgaaaagg acacaaacct
    10441 atataattgc gtcctctctt ttctagtttt acatattcac ttaccaagtc taaaatgtcg
    10501 gttttatctt ttatttcatt aatgatcgat tgatctattc gcaaattaaa tcacctatta
    10561 agcattagtt atactattat acctaatttt tgacaaaaat gaaatcattt cgatttattt
    10621 tgttcaatat aatggattat atcgtttgct gtttcttcga ttgctttttg agaaacatca
    10681 atgacaggac atccaatttc acttacgatt tcttcaaagt aattcaattc ttcttggatt
    10741 cgagcttctg ttgcataacg agctgtgtca cctagtccca attgttttaa tcgctctttt
    10801 ctaatgcgat ttaatttttc ttcacttatt ttaagtgcga tacatttctt aggatcaata
    10861 tcatataagc catctggcgg tgtcacttct ggtacaatcg gtacattcat aactttgtaa
    10921 ctcttatgcg ctaaatactg agataatggt gtctttgaag ttctcgaaat accaagtaaa
    10981 acaatatcag ctttaggtaa tcctttagga tctttaccat catcatattt aaccgcaaac
    11041 tctatcgcat caattttctt gaaatatgca tcatctaatc tatgaacgat acctggctca
    11101 ttataaggtt tttcttcaac cgatgctgat aataaatcca ttaatggccc catgatatca
    11161 acggacttca attggaattc tgctactttc tcactcatat attgcttcat ttcaggttta
    11221 ataagtgtat aaacaatgat agcatttgta tcttttgcaa cttgaatcac ttcatcaaca
    11281 tcttcaaaag attcaatata tggatatctt aataattcat ttttacattg cttaggatta
    11341 aattgtgaaa tacctgccct agcaactaac tctgccgttt cacctataga atctgaggct
    11401 acgataattt taattttttc cattacttct cacctattct ttatataatg ctactaataa
    11461 cttagctatt gttgttttgg aaattcttcc aattacttca tacttttgat tatctttttt
    11521 tcttacaatt ggaatcgaat caatttcttt ttcaatcatt ctatctgctg cgtatatgac
    11581 taattcgctt tcctctaaat aagtgacatt aggcatacgt gtcatattta cactgatagg
    11641 tactgtatga atatctgctc caatcattga agctcttaat aaatcttttc ttgaacacac
    11701 accaacaaaa tcgttatctt cattaataat aaataatgta cttacatctt ctaaaaaaat
    11761 tgtacaaata gcgtcataaa ctgttgtatt ctctcttagc acaacaggtt gagacatata
    11821 gtccttaacc tcaaattgtc gaagtttttc attaaaaaat ttgccttttg atttacctga
    11881 ataataatat ccaactcggg gacgcgcttc taaaaaacct gacattgtta atatcgctaa
    11941 atctggtcta agcgttgctc ttgttaaatt caacttatct gctatttgtt caccagtaat
    12001 gggtccttta gttttaacaa tttcgatgat tcgttcttgt ctttgactga gttctatagg
    12061 tcttcacccc ttttttattc acttattatt ataccgatat ttctttcatg tcacaaacac
    12121 attaatttta cttgccttta aataatctat caattagaat aaacgtacag cgagttaagg
    12181 atagtgtaag cttaacaata agattggcgc aacgaatcat tttaaaataa aagcgagtga
    12241 ctacactaat ttgggtggaa ccgcgggtta aatacgtaac ttattttgta ttatatacaa
    12301 tttaaaatat taactcgtcc catggaagta aagcatattt actatgctgt tattttccat
    12361 ggggcgtttt catgtatgag gagaggtaat tatggcaaaa gatatggata caattgtttc
    12421 attagcaaaa cacagaggtt ttgtgttccc tggtagtgat atttacggtg gtttatcaaa
    12481 cacatgggat tatggtcctt taggtgttga attaaagaat aatgttaaaa aagcttggtg
    12541 gcaaaaattc attacacaat caccgtttaa cgttggtatc gacgctgcaa tcttaatgaa
    12601 tccaaaagta tgggaagctt caggacactt aaacaacttc aacgacccaa tgattgataa
    12661 taaagatagt aaaattcgat atcgcgctga taaattaatt gaagattata tgcaagatgt
    12721 taaaggtgat gaaaacttca ttgccgatgg tttaagtttt gaacaaatga aaaaaattat
    12781 tgacgatgaa ggtattgttt gtcctgtaag taaaactgct aactggactg aaattcgcca
    12841 attcaattta atgtttaaaa cattccaagg tgtaactgaa gattctacaa atgaaatttt
    12901 cttacgtcct gaaacagcac aaggtatttt tgtaaactat aaaaacgtgc aacgttcaat
    12961 gcgtaaaaaa ttaccatttg gtatcggtca aattggtaaa tcattccgta atgaaatcac
    13021 tccaggtaac ttcattttca gaacaagaga atttgaacaa atggaacttg aattcttctg
    13081 taaacctgga gaagaaatcg aatggcaaaa ttattggaaa acttttgcaa gtgactggtt
    13141 aacaagctta aatatgagca gtgaaaatat gcgtttacgt gatcatgatg aagatgaatt
    13201 atctcattac tcaaatgcaa caactgatat tgaatataaa ttcccatttg gttggggtga
    13261 gttatggggt atcgcaagtc gtacagactt cgacttacgt aaacatgctg aacactctgg
    13321 tgaagatttc agataccatg atccagaaac gaacgaaaaa tatattccat attgtatcga
    13381 gccatcactt ggtgcagatc gtgtaacatt agctttctta tgtgatgcat atgatgaaga
    13441 aggcgttgaa ggtagtaaag atgcacgtac agttttacac ttccatcctg cattagcacc
    13501 atataaagca gcgattttac ctttaagtaa gaaattatct ggcgaagcga ttaagatttt
    13561 tgagcaatta agttctaaat tctcaatcga tttcgatgaa tcacaatcta tcggtaaaag
    13621 ataccgtcgt caagatgaaa tcggtacacc ttattgtgta acattcgact ttgattcatt
    13681 agaagataat caagttacag tacgtgacag agattcaatg gaacaagttc gtatgccaat
    13741 ctcagagtta gaagctttct taactgaaaa aacaaaattc taattaaaaa gactttccac
    13801 catatgtggt ggctatataa tttataaaaa gagatactgg ttagtaaaat tgccagtatc
    13861 tcttttctat tcaaaaaggt attcaaataa acacatctat gtggctactt gaataccttt
    13921 tattatttta tgttattgtt ccaatctttt taattggttg attagtttct gacttttaaa
    13981 aaacatacct gcatattcac gatataacat taaaatgata tctgacattt catcaataat
    14041 ttcttgatgg atattcaatg aattcatttt atctatcggt aatttttgta atacatctaa
    14101 taaatatagt gttttattcg ataatataac tgcatgtaca tctttagaag cctcttgcct
    14161 tgaaatcgca ccgtcaaact taaaactata acctattaaa tctgcttgtg tgtcattacc
    14221 actcacagca cagcgattaa atgatgcagt aaaaccaaat cgtttcatgc actttaacat
    14281 aactacgact gacattaact gtgcagatgt acctgattct attttttcaa gaacaaattg
    14341 taataattga tagttatatg gtgcaatgtc accttcatcc attgagcgct caatagtttc
    14401 agctgccaga gaggcataac tgcttacgta aaggtccatt tgtaatttat aatgttgact
    14461 aataacatct acagaattta acgttcccat acctcgccac tgattgtaaa taaacaaacc
    14521 ataaacaaac aattgcgttt gcgcttgtaa acccgtctta acttttttag cacgccttgc
    14581 cataagtggt acttttgcac catgctcatt taaaatcgtg ataattttat cagattcacc
    14641 ataatcaact gctttgatga taatcccttt ttggcgcatt aacaattatc ttcaccactt
    14701 ttaagattaa tcttggtctt caacataacc aatttggcga ataaagttaa ctttgtttcg
    14761 ccagtctctt tgaactttga cccataattc taagtatact ttagagccta gaagcatttc
    14821 tatatcacgt ctcgcacgtt ttcctacttc ttttaacttt ttaccgcctt ttccaatgac
    14881 aattcctttt tgcgaatctc tttcaacata tatagttgct tcgatatgaa cacgatcttc
    14941 gctttcttta accatacggt ccacattaac accaatcgca tgagggattt cttcacttgt
    15001 aagatgaagg attttttcac gaatgatttc acccactaca aattgttcag gatggtctga
    15061 aatttgatca tctggataat atttaggtcc ttcgggtaaa tacgtcttta aaacatcaat
    15121 aaaatgatct acatttagcc cttctaatgc tgaaataggt acaatctctg taaagtccat
    15181 ataactttga tattcttcaa tctttggcat taattcatct ggatgcacta aatctatttt
    15241 atttaatact aaaaatactg gtgtcttaac atttttcaac atttctataa tatattcatc
    15301 gcctcgtcca atttcctcat tggcattaac cataaacatg attgcatcta tttcagataa
    15361 tgtattttta gcgactttca tcatatagtc acctaatttg tgtttaggtt tatgaatacc
    15421 tggcgtatca atgaatataa tttgcgcgtc atctcttgtc ataacacctt gaattttatt
    15481 tctagttgtt tgagctttat cggacatgat tgctatttta tggccaatca ctctattaac
    15541 aaatgttgac ttccctacat ttggtctacc tataattgaa acaaatcctg atttatgttc
    15601 tgtcatttat tctaaatcct ttcctgaaaa tccaaatggt agtaactctg cgactgtcat
    15661 cataaccata tctcctttat gatttgtcat atacacaggc atatcatcat cacataattc
    15721 ctttaaaact tgacgacatg caccacaagg tgatgacggt ttatctgcat ctacggttac
    15781 agttattgat tcaaaatcac ctggtctgta tccttgagaa attgccgata ccaaactagc
    15841 tcgttcagca catatcgata atggataaga agcattttct acattggtac cataaaaagt
    15901 tctaccgtct ttcgttttta aataagcccc tactttaaat tgactgtatg gcgaatatga
    15961 ttcttgttgt gcttttctaa cttcttgaaa ataatgaggt tgataactca tatatgcctc
    16021 cctaaaataa cgctataaaa tgtggtaaaa atactattaa acctataata aatgctaata
    16081 ttgaaactat aagtacacta aaagccgcta tatcttttgc gtatttagct aaatcatgat
    16141 attcaacggt cactaaatcg acaacatatt caatagcagt gtttaaagct tcaacagtga
    16201 gaactaatgc aatagcaatg agtataaata tccactcaat acgattaata tttagtacga
    16261 gaccaaagac aatagcaaca atcattgcaa acacatgtaa aagaaattta tagtcttttt
    16321 gaattaagat tttcagccca tcaagtgcat atttaaacct tttcataatt agtctcgtgt
    16381 taatccataa gcgtttaata ttgtatcttg tcgaccaaac atttcctttt catccgcttc
    16441 agtcatatga tcataaccta atagatgcaa aaatccatgt aatgctaaaa atcctaattc
    16501 tcgttcaaaa gaatgtccgt aattgtttgc ttgttcttgc gctacatccg tacagataat
    16561 tatatcccct aaaacacgtg gtatatcaag accactaaaa tcaatctctg gctcatcttc
    16621 ttctaaagca aatgagatta catctgtaac tttatcttta tctctatatg ttcgattaat
    16681 ttcttgtatt tcttgtttat ctacaaatgt aacagaaagc tcagcatcgt cttctatatg
    16741 ctcttctttt ttagcaaatt ctaataaatc ttcaatttgt ttataccacg catctttaac
    16801 taagcctgtg tgatcgctaa aatctatcgt aaacatttaa ttctctcctt cataatgttc
    16861 aatgatctta cttaccaatg gatgtcttac tacatcgctc tgatctaatt tcaatatact
    16921 tataccttta acgttgtgta acctactgac cgcttcctta agtccacttt taacaccttt
    16981 aggtaaatcg atttgagttt ggtcaccagt aactaccatt tttgagccaa aacctagtct
    17041 tgttaaaaac attttcattt gcgcatgtgt cgtattctgc gcctcatcaa gaattacaaa
    17101 tgcatcttct aatgttcgtc cgcgcatata tgcaagtggc gctatttcga taatgcctct
    17161 ttcaataaat cgctctgttt gttcacgccc aagaacagta tatagaccat catataaagg
    17221 tcttaaatat ggatctacct tttctttcaa atctcctggt aaaaatccaa gtgactctcc
    17281 tgcttcaaca gcaggtcttg ttaatacaat acgtttaaca gcacctttac ggagttgctt
    17341 tgctgcataa actacagcta agaatgtctt acctgtacca gcaggaccta taccaaatac
    17401 taaatcatta tttttcatgg cattaacata tatacgttgc cccatcgttt tcgcacgaat
    17461 cgtcttacca aatgcatctt tagttatctc ttcatcatat aaatctaaca gatgttgaat
    17521 tgtgttatta tgcgccattt taatagctgc ttcaacatct ttaattgtaa tattattacc
    17581 taaatcaata accttcagca aattgattaa tactgattcc gctttttcta cgttttctat
    17641 ttttgtacct ttaacggcaa cttcttgtcc tcttgcatgg atgacaacat cgaaactctc
    17701 ttcaattgct tttaaatgtt catcattatt tccaattaaa gcttgagatt ggttcatatc
    17761 gtctatttgt ataattccag gcatacacgc gctccttttc atatatcatt tatattcatt
    17821 ctatgatgct ttaatat
//
LOCUS       DAOBBH010000038        17811 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.038, whole genome shotgun sequence.
ACCESSION   DAOBBH010000038 DAOBBH010000000
VERSION     DAOBBH010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17811
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.038"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(426..872)
                     /gene="perR"
                     /locus_tag="P6L01_000946"
     CDS             complement(426..872)
                     /gene="perR"
                     /locus_tag="P6L01_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HDP3030031.1"
                     /translation="MSVEIESIEHELEESIASLRQAGIRITPQRQAILRYLISSHTHP
                     TADEIYQALSPDFPNISVATIYNNLRVFKDIGIVKELTYGDSSSRFDFNTHNHYHIIC
                     EQCGKIVDFQYPQLNEIERLAQHMTDFDVTHHRMEIYGVCKECQDK"
     gene            complement(969..1919)
                     /locus_tag="P6L01_000947"
     CDS             complement(969..1919)
                     /locus_tag="P6L01_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDP3030032.1"
                     /translation="MKVVGLNRMREVETELQQRFSDLDFKFYKKASEIPESDLADLDI
                     LVGYDGGINEAFLQRCPNLKWIAWFATGVNTLPLDYIADHGILLTNGKGVQAKQLSEY
                     ILAFILDDYKKMKLSYDNQRQHIYDSKITGKRLSGQTVLFLGTGAIATRTAKLAKAFN
                     MNLIGLSKSGQNKDEFDEIYTIESLESTLPNADIIINALPETQETIHLLKKKHFELMK
                     DEALFINIGRGSIVKEALLIEVLKSKVIRHAYLDVFENEPLKPNHELYELENVTITAH
                     ITGNDYEAKYDLLDIFKNNLVNFLNKNGLIENEVDAKKGY"
     gene            complement(1925..2380)
                     /gene="bcp"
                     /locus_tag="P6L01_000948"
     CDS             complement(1925..2380)
                     /gene="bcp"
                     /locus_tag="P6L01_000948"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDP3030033.1"
                     /translation="MLQKGEQFPIFKLENQDGTVITNDTLKGKKAIIYFYPRDNTPTC
                     TTEACDFRDNLEMFNDLDVAVYGISGDSKKKHQNFIEKHGLNFDLLVDEDFKLAKETG
                     VYQLKKSFGKESMGIVRTTFIIDEQGKVLDVIEKVKVKTQIEELKNILG"
     gene            2461..3750
                     /locus_tag="P6L01_000949"
     CDS             2461..3750
                     /locus_tag="P6L01_000949"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDP3030034.1"
                     /translation="MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHG
                     AYLYDVDGNKFIDYLQAYGPIIAGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSKKL
                     RDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGP
                     SQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMP
                     QPGFLEEVNEISHNNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPI
                     GGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQL
                     EEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQG
                     INLAPSKFEAWFLTTEHTEEDIQQTLKAADYAFSQMK"
     gene            4043..5137
                     /locus_tag="P6L01_000950"
     CDS             4043..5137
                     /locus_tag="P6L01_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDP3030035.1"
                     /translation="MRLGARIFKTGIAIILAMSIASLLPDDVGLKALAGVSAVVAMQP
                     SIYRSFKTVSDQALGNIIGAILSVTMVTIFSDNFIIMGVTVIVLIAILFKFNLAHVAT
                     LASVTALIIMGQHTGSFYITAFYRFVLVMIGVISSSLVNFVFLPPKFETKIYYNSLNI
                     SSDIFMWFKLVLNDTTEFNNIKQDSHNLKQRVEKLEKIYDYYSEERPITKKHIHQQNR
                     KKILFREVVQTTRQAYEVLNKLSRYQNDLYLLNNNFLLQIKLDLDSLTAFHEQILASL
                     SKKARYNVTHVDYELDNPQKKDLLSTFQHELINHPYQTEYSFANVMQIVAAIEEYRHH
                     LEHLDRIRISFFTYHRSDADIEIVEEDFDL"
     gene            complement(5227..5318)
                     /locus_tag="P6L01_000951"
                     /pseudo
     CDS             complement(5227..5318)
                     /locus_tag="P6L01_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500499.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5327..7063)
                     /locus_tag="P6L01_000952"
     CDS             complement(5327..7063)
                     /locus_tag="P6L01_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV1866 family putative multidrug efflux ABC
                     transporter"
                     /protein_id="HDP3030036.1"
                     /translation="MIKRYLQFVKPYKYRIFATIIVGIIKFGIPMLIPLLIKYAIDGV
                     INNHALTTDEKVHHLTIAIGIALFIFVIVRPPIEFIRQYLAQWTSNKILYDIRKKLYN
                     HLQALSARFYANNQVGQVISRVINDVEQTKDFILTGLMNIWLDCITIIIALSIMFFLD
                     VKLTLAALFIFPFYILTVYVFFGRLRKLTRERSQALAEVQGFLHERVQGISVVKSFAI
                     EDNEAKNFDKKNTNFLTRALKHTRWNAYSFAAINTVTDIGPIIVIGVGAYLAISGSIT
                     VGTLAAFVGYLELLFGPLRRLVASFTTLTQSFASMDRVFQLIDEDYDIKNGVGAQPIE
                     IKQGRIDIDHVSFQYNDNEAPILKDINLSIEKGETVAFVGMSGGGKSTLINLIPRFYD
                     VTSGQILIDGHNIKDFLTGSLRNQIGLVQQDNILFSDTVKENILLGRPTATDEEVVEA
                     AKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLSIARIFLNNPPILILDEATSAL
                     DLESESIIQEALDVLSKDRTTLIVAHRLSTITHADKIVVIENGHIVETGTHRELIAKQ
                     GAYEHLYSIQNL"
     gene            complement(7354..7896)
                     /locus_tag="P6L01_000953"
     CDS             complement(7354..7896)
                     /locus_tag="P6L01_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDP3030037.1"
                     /translation="MVRESIPKEGENIKIQSYKHDGKIHRVWSETTILKGTDHVVIGG
                     NDHTLVTESDGRTWITREPAIVYFHSEYWFNVICMFREDGIYYYCNLSSPFVCDEEAL
                     KYIDYDLDIKVYPNGKYHLLDEDEYEQHMNQMNYPHDIDIILRRNVDILQQWIEQKKG
                     PFAPDFIKVWKERYKKIRQY"
     gene            complement(8199..9236)
                     /gene="mutY"
                     /locus_tag="P6L01_000954"
     CDS             complement(8199..9236)
                     /gene="mutY"
                     /locus_tag="P6L01_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDP3030038.1"
                     /translation="MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQ
                     VKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVYDKYEG
                     LVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQST
                     RKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKL
                     PVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRSEKLLHGMWQFPMFESEHARRKMTEKI
                     GHDIQPVETPIFELKHQFTHLTWKIKVYAASGAINIETLPDDMIWFNLSDRDQYTFPV
                     PMSKIYQFING"
     gene            9388..10365
                     /locus_tag="P6L01_000955"
     CDS             9388..10365
                     /locus_tag="P6L01_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDP3030039.1"
                     /translation="MDTATHIAIGVGLTALATQDPAMASTFGATATTLIVGSLIPDGD
                     TVLKLKDNATYISHHRGITHSIPFTILWPILITFLIFTFFSGTNPFHVWMWAQLAVFL
                     HVFVDIFNSYGTQALRPITNKWIQLSVINTFDPIIFTVLCIGIVLWVIGLHPFAVFFP
                     IIALLIIYYMIRFKMRAVIKQQALKAIQQEHHPVKVFVAPTIKFMEWRVAIQTDAHDY
                     VGKAYGRNVVFSDKVERQTLSTDSILWKVKGNKDIRTFLNFSSIYRWQTTTLADGSTE
                     IRLIDLRYLKNDHYSFVAIAHVTNDNVIDHSYIGWVFTEDKLQRKLYAK"
     gene            complement(10626..11462)
                     /locus_tag="P6L01_000956"
     CDS             complement(10626..11462)
                     /locus_tag="P6L01_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030040.1"
                     /translation="MLDNIILYFKNLPHTKRYVTERLKQSWKSFLIVLAACLILIIAS
                     ETLFSFSHLTDVKEVRWLFRIIALIVFAVLMFTIYISYHHYMNDFLVTKLFNISAATP
                     VVIMSILSFIMLVILTMISALVKPVNFETSYIALFYFIVMATIFVGLISVTFGLIRLL
                     TEKINIIFYGVCVLCILLLPIIFIPNPNHVFINHILMLNPMYYIVNGIAQSIIFGISS
                     MENIPYHFYFILFLCLIAAVNFVLARYTTHAIYNKTSKVTQTDNQQDVSNDSTDEADT
                     SS"
     gene            complement(11471..12988)
                     /locus_tag="P6L01_000957"
     CDS             complement(11471..12988)
                     /locus_tag="P6L01_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3030041.1"
                     /translation="MGSSIVLKLLKVTHYYRNKQNKKWYLPFGYDAEDIDLNNISLHI
                     YQGEALGIIGEPESSKALVGQLLAGAIKPDKGKVVCTEDLFYGYIEDQSLIHQTVEAY
                     TAQLVQLFPYEINDHKAEQIIQYAHLGDYKTKPVNHISKAAYAQLLLSIARSSKSNII
                     ILNHVIDYLTPQFMERAIELTNDYIENNLTIVSIGDDIDKISQVSNYIAWFSHGQLRM
                     EGSLKQVIPSFKEHERDRLSLNSKEEIENFDLDWKKNRTRIPEMTYNFKRVERYNHAK
                     PPKFLVRFWTLASGTILGLALMMLLIFNNIGIISITDFTNRATMQNENKDPYGEKLAY
                     GIAFNGSVDMQGDKQVTIPKYSVVTITGENSKNYRVTADNKTYYVSKDKLEYFNPAGL
                     YQTHSFKKLAPYMKSNYSNYYAYFNSQLHKKHSSVIKTLVPDDDNRFVASVTQQPIQL
                     LFNDNNQLYGFVYPIVDKKELKDKFNINNNIWITKVGNGYCIANLKEDKWIYIEL"
     gene            complement(13003..13317)
                     /locus_tag="P6L01_000958"
     CDS             complement(13003..13317)
                     /locus_tag="P6L01_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhH family protein"
                     /protein_id="HDP3030042.1"
                     /translation="MEQKKLSEMSEPELRHEIQLYKEKMRKAEMNGILNEYDVYQSKV
                     IVAESYLVDRKKIEIGKIYKLTDGSNQYFKVERLKGIFAWGFRFNSDEPEEGLPIALL
                     QL"
     gene            complement(13295..14113)
                     /gene="recX"
                     /locus_tag="P6L01_000959"
     CDS             complement(13295..14113)
                     /gene="recX"
                     /locus_tag="P6L01_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDP3030043.1"
                     /translation="MPKITKIEVQKKNKERFNLFLDEQFEMGIDIDTLVKFNLKKGQQ
                     LEAADMAEIQKYDHYRIGLNKAIQYLSYKKRTEKEVIQYLQKEEISEQAISEVIEYCY
                     REKLIDHQDYAESLKNTMIRTTDKGPKIYQQKLYQLGIEPNIIEMFTELYREQQELDD
                     IIQIAEKISKTKKGPQNKVKEKVMQSLIQKGFEMGTIHAVLNEMDFTQDEAVLDDLLQ
                     RDLEKIYNKNRKKYTQQKLISKTIEGLMRKGYKYDKIKAKLEESGIADGTEEIE"
     gene            complement(14373..15182)
                     /gene="sgtB"
                     /locus_tag="P6L01_000960"
     CDS             complement(14373..15182)
                     /gene="sgtB"
                     /locus_tag="P6L01_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional peptidoglycan glycosyltransferase
                     SgtB"
                     /protein_id="HDP3030044.1"
                     /translation="MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILL
                     KILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDE
                     RFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVA
                     HRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAI
                     LASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR"
     gene            complement(15522..16037)
                     /locus_tag="P6L01_000961"
     CDS             complement(15522..16037)
                     /locus_tag="P6L01_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDP3030045.1"
                     /translation="MTKKVAIILANEFEDIKYSSPKEALENAGFNTVVIGDTANSEVV
                     GKHGEKVTVDVGIAEAKPEDYDALLIPGGFSPDHLRGDTEGRYGTFAKYFTKNDVPTF
                     AICHGPQILIDTDDLKGRTLTAVLNVRKDLSNAGAHVVDESVVVDNNIVTSRVPDDLD
                     DFNREIVKQLQ"
     gene            complement(16168..16329)
                     /locus_tag="P6L01_000962"
     CDS             complement(16168..16329)
                     /locus_tag="P6L01_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030046.1"
                     /translation="MNEQQTIEQIKARLNKFIEDIDHVNPDEVRVEDIDEWIGLLDQL
                     EEKVKLVSK"
     gene            16553..17704
                     /gene="yfkAB"
                     /locus_tag="P6L01_000963"
     CDS             16553..17704
                     /gene="yfkAB"
                     /locus_tag="P6L01_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/CxCxxxxC motif protein YfkAB"
                     /protein_id="HDP3030047.1"
                     /translation="MNSVKLKQPVSIYNDPWESYNDVKEHGQLTLSNIEFTTTNLCNM
                     RCSHCAVGYTLQTVDPEPLDMDLIYRRLDEIPNLRTMSITGGEPMFSKKSIRNVVKPL
                     LKYAHHRGIYTQMNSNLTLPQDRYLDIAEYIDVMHISHNWGTTDEFANVGFGAMKKQP
                     PLKAKLKLYEQMISNARTLSEQGMFVSAETMLNQSTLPHLRKIHQEVVHDMKCSRHEI
                     HPMYPADFASQLNVLTLAEMKKTIHDILDFRDEDIWMLFGTLPVFPCLKDDEDQKLLS
                     RLRNANNVTTRNDPDGRSRLNVNVFTGNVIVTDFGDETGTISNIQKDKLTDVFDKWLS
                     SDLAKSLNCHCSEFSCLGPNVLVKNMYYPNMDFKDNERHMHKQPQIIQF"
BASE COUNT         5836 a         3039 c         2487 g         6449 t
ORIGIN      
        1 tgccgtttga cgacttttat ataatatcaa gttattctag tagagtcaac ataaaacaac
       61 acttattttt gtttaaaaat tactgttgaa gggattattt tctattcgta agtactatat
      121 gtatttaaag tgtaattcct tacgcttttt acttcagttt tacaaacaaa cttatactca
      181 ctttatggat agttatttag tagaccttcg tttgaatatt tttatgttta tttataaagg
      241 gtactagtta ttagttaata ccacttccgt agttagaata ttcttttctt caatttaaat
      301 atttttcaga ataagcataa aaaagaagtt aaacaacttt catgtcgttt aacttcttaa
      361 atattacact tacttatatg aagtaagcta acgctttttt aatttgtcat actaccaaag
      421 ttaaattatt tatcttggca ttctttacaa actccataaa tttccattcg atgatgtgtt
      481 acgtcaaagt cagtcatatg ctgagctaat ctttcaattt catttaactg tggatattga
      541 aaatcaacaa tcttaccaca ttgttcacat ataatatgat aatgattatg tgtattaaag
      601 tcgaatcgac ttgatgagtc tccatatgtt aattctttta caattccaat atctttaaac
      661 actcttaagt tattatatat tgtcgcaaca cttatatttg gaaaatcagg tgaaagtgct
      721 tgataaattt catcagctgt tggatgagta tgtgaagaaa ttaaataacg taatattgct
      781 tgtctttgag gtgtaattct tatgcctgct tgtcgcaatg atgcaattga ttcttctagt
      841 tcatgttcaa ttgattctat ttcaacactc atctatatca ccatctttct tatataataa
      901 ttattattac ttactataaa tataactgta gtattgcgcg cgtgtcaata tttacatgat
      961 gatttcattt aatagccttt tttagcatca acttcattct caattagacc attcttattg
     1021 agaaaattaa ctagattgtt tttaaaaata tctaataagt catactttgc ctcataatca
     1081 ttaccagtta tatgcgctgt tatagttaca ttttccaatt catataattc atgattaggt
     1141 ttcaaaggtt cattttcaaa cacatctaaa tatgcatgtc gaataacttt actttttaat
     1201 acttctatta agagcgcttc tttaactatg ctacctcgtc ctatatttat aaaaagtgct
     1261 tcatctttca ttaattcaaa atgttttttc tttagtaaat gaatcgtttc ttgcgtttct
     1321 ggtaaagcat ttataataat gtcagcattt ggtaatgtgc tttcaagtga ttcgatagta
     1381 tatatctcgt caaactcatc tttgttctga cctgacttgc tcagaccaat taaattcata
     1441 ttaaaagcct ttgctaactt cgcagttcta gtagcaattg cacctgtacc taaaaataaa
     1501 actgtttgtc ctgataggcg tttaccagtt attttcgaat catatatatg ttgtctttgg
     1561 ttatcatatg atagtttcat ctttttataa tcatctaaaa tgaaagctaa aatgtattca
     1621 gataattgtt tagcttgaac accttttcca ttagttaaaa gtatgccgtg atctgcaata
     1681 taatctaacg gtaatgtatt tacacccgtt gcaaaccatg caatccattt taaattcggg
     1741 caacgttgta aaaatgcctc attgataccg ccatcataac caactaatat atctaaatca
     1801 gccaagtcac tctcaggtat ttctgatgct tttttataaa atttaaaatc taaatctgaa
     1861 aagcgttgtt gtaattcagt ttcaacttca cgcatacgat ttaacccaac aactttcata
     1921 tatgtcaccc caaaatgttt ttaagttctt ctatttgtgt tttaacctta accttctcga
     1981 taacatctaa tactttacct tgttcatcta ttataaaagt tgttcttaca atacccatac
     2041 tttctttgcc aaatgatttt tttaactgat atacgcctgt ttctttagct aatttaaaat
     2101 cttcatctac taataaatcg aaattcaatc cgtgtttctc aataaaattt tggtgttttt
     2161 tctttgaatc accgcttata ccatatactg caacatctaa atcattgaac atttctaaat
     2221 tgtctctaaa gtcacaagct tctgtggtac aagtaggtgt attatctcta ggataaaaat
     2281 atataatcgc ctttttacct tttaatgtat catttgtaat gacagttccg tcttgatttt
     2341 ctaatttaaa tattggaaat tgttctcctt tttgcaacat ataatcacct ttttcttcat
     2401 tataataatt ttatgatacg atattagatg aaaatattaa attaaaagag gttgatataa
     2461 atgaatttta gtgaaagtga acgtttacaa caactttcaa acgaatatat tctaggcggt
     2521 gtcaattccc cttctcgttc ttataaagct gtaggaggcg gtgcacctgt tgttatgaaa
     2581 gaaggacacg gtgcatattt atatgatgtc gatggcaata aatttattga ttaccttcaa
     2641 gcatacggtc caattattgc ggggcatgca catcctcata ttactaaagc aattcaagaa
     2701 caagcagcca aaggtgtttt atttggtaca ccgactgaac tagaaattga attcagcaaa
     2761 aaattacgtg atgcaattcc atctcttgag aaaattcgct ttgtaaattc tggaacagaa
     2821 gcagtcatga caacaattcg tgttgcacgt gcatatacta aaagaaataa aattataaaa
     2881 tttgctggat cttatcatgg ccattctgat ttagtattgg ttgcagcagg tagtggccca
     2941 tctcagctcg gttctccaga ctcagctggt gttccagaaa gcgtcgcacg tgaagtcatt
     3001 actgtacctt tcaatgatat taacgcatat aaagaagcaa ttgaattttg gggtgatgaa
     3061 attgccgcag tattagtaga accaattgtt ggtaactttg gaatggtaat gcctcaacct
     3121 ggatttttag aagaggttaa tgaaatttcg cataacaatg ggacactagt gatttatgat
     3181 gaagtaatta ctgcattccg tttccattac ggtgccgctc aagatttatt aggtgttatc
     3241 cctgatttaa ctgcatttgg taaaattgtt ggcggtggtt taccaattgg aggctatggt
     3301 ggacgtcaag atattatgga acaagtagca cctctaggac ctgcatatca agctggtaca
     3361 atggctggta acccgttatc tatgaaagca ggtattgcat tactcgaagt actagaacaa
     3421 gacggtgttt atgaaaaatt agacagctta ggccaacaac tagaagaagg tttacttaaa
     3481 ttaatcgaaa aacataatat cacagctaca attaatcgta tttatggatc tttaacattg
     3541 tactttacag atgaaaaagt cacacattat gatcaagttg aacattctga cggcgaagcg
     3601 ttcggtaaat ttttcaaatt aatgttaaat caaggtatca atttagcacc ttctaagttt
     3661 gaagcttggt tcttaacaac tgaacataca gaagaagata ttcaacaaac tttaaaagct
     3721 gcagactatg cttttagtca aatgaaataa cttttaagga agtatatttt taattgaatt
     3781 aaaataaaac aaatgctcac atagtagatt cattttatat cgttaaatat taacgtttaa
     3841 cttgatatgt cactatgtga gtttttattt tacaaattta tactctttta atctctgact
     3901 aaagttttac atcgctatca gtattatgta tgatttattt taacaactat aaataagctt
     3961 gaattttata actagtaagt gtataacagt aatgaatacg aatattagat attcaataaa
     4021 cttaaaaggg gcgtcattat ttttgagact aggagctcgg atttttaaaa ctggtatagc
     4081 cattatttta gctatgtcta tcgcttcttt actaccggat gatgttggtc tgaaagcctt
     4141 agctggtgtc agtgctgttg ttgcaatgca acctagtatt tatcgttcat ttaaaacagt
     4201 ttctgatcaa gcattgggta acattatagg tgccatatta tctgttacaa tggtaacaat
     4261 ttttagtgat aattttatca ttatgggtgt taccgtcata gtacttattg ctattttatt
     4321 taaatttaat ttagcacatg ttgctacttt ggcgagtgta acagcattaa tcattatggg
     4381 acaacatacc ggttctttct atattaccgc cttttacagg tttgtacttg taatgattgg
     4441 tgtcataagt tcatctctag ttaactttgt gtttctacca cctaaatttg aaacaaaaat
     4501 ttactataat tcattaaata tatcctcaga tatttttatg tggtttaaat tagtattaaa
     4561 tgatacgacg gagtttaata atattaaaca agatagtcat aatttaaaac aacgtgtcga
     4621 aaagttagag aaaatttatg attactatag cgaagaacgt cctattacaa aaaaacatat
     4681 tcaccaacag aatagaaaga aaatactttt cagagaagta gttcagacga ctagacaagc
     4741 ttatgaagta ttaaacaaat tgtcgcgtta tcaaaacgac ttatatttac ttaataacaa
     4801 tttcctatta caaatcaaat tagatttaga ttcattaact gcttttcacg agcaaatttt
     4861 agctagcctt tctaaaaaag cacgttataa tgtgacacat gttgattatg agttagataa
     4921 tccgcagaaa aaagatttat tgtctacatt tcagcatgag ttaataaatc atccttatca
     4981 aacggaatat tcgtttgcca atgttatgca aattgttgca gccatcgaag agtatcgaca
     5041 ccatttagag catttagacc gcataagaat tagctttttc acatatcata ggtctgatgc
     5101 agacattgaa attgttgaag aagactttga tttataagtt taaagcaatt cctgtaatta
     5161 ataccacaat gagataaagt aatattgaat ctcgaaatta aatttaaaaa agcagtaaga
     5221 ttattttcaa tgaagaaaat ctcttactgc ttttctatgt tatgacccca ccatcctgtt
     5281 acaaatcttc cctacaagat tgtactgaag tcaaacaact aatactttat aagttttgaa
     5341 tgctatataa atgctcgtaa gcaccttgtt ttgcaatcaa ttcacgatgc gtacctgttt
     5401 caacaatatg tccattttca attacgacaa ttttgtcagc atgtgtaata gtggacaagc
     5461 gatgcgctac gataagtgtc gttcgatctt tactcaacac atctaatgct tcttgaataa
     5521 tggattcact ttctaaatca agtgcacttg ttgcttcatc caagataaga attggcggat
     5581 tatttaaaaa tattctagca atcgataatc tttgtttttg accacctgat aatttaacac
     5641 ctcgttcacc tacttcagtg tcatatccct gtggcaagtt cataataaag tcatgtgcat
     5701 tagccatttt cgccgcttca actacttctt catctgttgc tgttggacga ccaagtaaaa
     5761 tattttcctt aactgtatca gagaataaaa tattatcctg ttgcaccaat cctatttgat
     5821 ttcttaaact tcccgttaaa aaatctttaa tattgtgacc atctattaaa atttgcccag
     5881 aagttacatc gtaaaatctc ggtattaagt taattaatgt tgatttacca ccaccactca
     5941 tacctacgaa agcaactgtt tctccttttt caatactcaa attaatatct tttaaaattg
     6001 gagcttcgtt atcgttatat tgaaaactaa catgatcaat atcaatacga ccttgtttaa
     6061 tttcaatagg ttgagcacca acaccatttt taatgtcata atcttcgtca attaattgga
     6121 atacacggtc cattgaagca aaactttgcg ttaaagttgt aaatgatgcg actaaacgac
     6181 gtaaaggccc gaacaataac tctaagtatc caacaaatgc tgcaagtgta cctactgtga
     6241 ttgatccaga aatagcaaga tatgcaccaa caccgatgac aataattggt ccaatatctg
     6301 taactgtatt aattgcggca aaggaatagg cattccatct tgtatgtttc aacgcacgtg
     6361 ttaagaaatt agtattcttt ttatcaaagt ttttcgcttc attgtcttca atcgcaaaac
     6421 ttttaacgac tgaaatacct tgaacacgtt catgcaagaa tccttgaacc tcagctaatg
     6481 cttgcgatct ctcacgcgtc aattttctta atcttccaaa gaaaacgtac accgttaaaa
     6541 tgtaaaatgg gaagataaac agtgctgcta aagtcaattt cacatctaaa aagaacatta
     6601 tggatagtgc aataataatt gttatacaat ctaaccaaat attcattaac ccggttaaaa
     6661 tgaaatcttt tgtttgttca acatcattaa tcactctaga tattacttga cctacttgat
     6721 tattagcata aaatctcgca cttaaagctt gtaaatggtt atataacttt ttacgtatat
     6781 catacaatat tttattactt gtccattgcg ccaaatattg acgtataaat tcaattggtg
     6841 gtctcactat tacaaaaata aataatgcga taccaatggc aatagttaaa tgatgaactt
     6901 tttcatcagt cgttagtgcg tggttattaa tcacgccatc tattgcatat ttaattaaaa
     6961 gtggtataag cattggtata ccaaacttaa ttatcccaac aataatcgtt gcaaaaatac
     7021 gatatttata tggcttaaca aattgcaaat atcgtttaat catacaatcc cccctaatct
     7081 attgccctat cctattcata agcataaaaa tgaatagagg ttggatacat aatttgtaga
     7141 tgtaaattct tcttacaatt tacattttta aaattaagat atctcaacct ctgtcatatt
     7201 catgtgatta aatacgttgt cttattcgta tgttatattc tattcaattt aatctatgga
     7261 tactgtgtcc ccacacgaca gcaaaagtta tcatacttct ttacatcact aagtcaatat
     7321 aaatgattta atcagtattt acactttatt tgcttaatac tgtctaattt ttttgtaacg
     7381 ttctttccaa actttgataa aatctggcgc gaatgggccc ttcttctgtt ctatccattg
     7441 ttgaagaatg tccacgttgc gtcttaaaat aatatcaata tcatgcggat aattcatttg
     7501 attcatatgt tgctcatatt catcttcatc taataaatga tactttccgt ttggatatac
     7561 tttaatatct aaatcatagt ctatatattt taatgcctct tcatcacaaa caaatggtga
     7621 tgacaaattg caatagtaat aaattccatc ttctctaaac atgcagataa cattaaacca
     7681 atattctgag tgaaagtaaa caattgccgg ttcacgtgtt atccaagttc ttccgtcact
     7741 ttcagtcact aacgtatgat cattgccacc aatgacaaca tgatcagtac cctttaatat
     7801 tgttgtttca gaccaaacgc gatgaatctt accatcatgt ttataactct gaattttaat
     7861 gttttcccct tctttaggta tggattctct gaccatactc cacaccacct tctgttaatt
     7921 taaccattat aaattatagc atatttcaga aatagtatta tataaataca tatttttacg
     7981 aaataagatt ttactactta atgattaaac tcggtaatat tgctaagtac tacaacagag
     8041 atttacatgt cccatttaaa gtatgtaaaa tcatcacttt tataaatcaa cactttaact
     8101 ttttgacatt gttatcctat gagatttaaa gatatcattt atacattttt aaaattaatg
     8161 tcactatgtt ttccgataat attaccaatc atcgaatgtt acccatttat aaattgataa
     8221 atctttgaca taggtacagg gaatgtatat tggtctcgat cacttaaatt aaaccaaatc
     8281 atgtcatctg gtaatgtttc aatgttaatt gctcctgaag cggcgtatac tttaatcttc
     8341 catgttaaat gagtaaattg atgcttcaac tcaaaaatag gtgtttctac tggttgaatg
     8401 tcatgaccga ttttttcagt cattttacgt ctagcatgct cactttcaaa cataggaaat
     8461 tgccacatac catgcaataa tttttcgcta cgcttttgca acagatattg accttgatta
     8521 tttctaatta aaaagacgga ttgctcaatt acttttttac ttacattttt agatttaaca
     8581 ggtaactttt caaatgtacc tttatcaaat gcctcacagt tttcttgaac tggacaaaat
     8641 aagcataatg gattttttgg tgtacaaatt aatgccccta attccatcat agcttgatta
     8701 aacgttccag cttctgtagt aacatacggt aacaattctt gttcgtacga tttcctcgtc
     8761 gattgtaatt taatatctcg atagtcatca ttcaatctag accatactcg aaaaacattt
     8821 ccgtctacag ttgctagtgg tacattatat gcaatgctca ttactgcagc ttgtgtgtat
     8881 gggccaacac cttttaacgc tttaaattga tcaggatctt tgggaactaa gccttcatat
     8941 ttatcataaa cttctttaat cgccgtatga aaatttcgag ctctactata atatcctaag
     9001 ccttcccaat actttaacac ttcatcttcc gaagcttgac tcaaaacttc cacagttgga
     9061 aatcgttcaa caaaacgatg ataatagtca ataactgttt taacttgtgt ctgttgtaac
     9121 atgacctcac ttaaccaaat atagtacgga ttggtcgttt gtcgccatgg catttctctt
     9181 tgattttcat caaaccagtg tatcaaattt tctttaaaac tagactgctg atacatttat
     9241 aaaacccttt cctcaccaaa attaattgtc tttactcata atgtttttat tgtacattaa
     9301 aatcatggtt agtatgtaag ttaatttagt tatttgcgaa attggattat aatagtatat
     9361 ataatattat gaaatgagtg aactgatatg gacactgcaa cacatatcgc aattggggtg
     9421 ggccttacag cacttgcaac tcaagatcca gcaatggctt ctacgtttgg tgcaacagct
     9481 acaaccctta tcgttggttc attaattcct gatggggata ctgttcttaa attaaaggac
     9541 aatgcaacat atatttcgca tcatagaggt atcacgcatt ccatcccttt cacaatacta
     9601 tggccaattt taattacatt tttaatattc acgttcttta gtggaaccaa cccatttcat
     9661 gtatggatgt gggctcagct cgcagtattt ttacatgtct ttgtagatat attcaattct
     9721 tatggtacac aagcgcttag acctatcaca aacaaatgga ttcaattaag tgtgattaac
     9781 acatttgacc ctattatttt cacagttctt tgtattggta ttgtattatg ggttataggc
     9841 ttgcatccat ttgcagtctt ctttcctata atcgctttac taatcattta ttacatgatt
     9901 cgttttaaaa tgagagccgt aattaagcaa caagctttaa aagcaattca acaagagcat
     9961 caccctgtta aagtatttgt tgcgccaaca ataaaattta tggaatggcg tgtcgcgata
    10021 caaactgatg cacatgacta tgttggaaaa gcatatggta ggaatgtggt gtttagtgat
    10081 aaagtggaac gtcaaacatt atcaacagac tccattttat ggaaagtcaa aggtaataaa
    10141 gatatacgta cttttttaaa cttttcatca atctatcgtt ggcaaacaac aacgttagca
    10201 gatggttcta ctgaaattcg tttgattgat ttgcgttatt taaaaaatga tcattattca
    10261 tttgtggcaa ttgcacatgt aacaaacgat aatgtcatag accactctta tattggctgg
    10321 gtatttacag aagataagtt acaacgtaaa ctgtatgcta aataatttca agttattatt
    10381 cactaaagtt aatctataaa aaatgaacaa ccgggcagaa tgaaaatcaa aacgattttt
    10441 actctgtccg gttttttaat gtaaaactat gaatgctttt acaaaatcta aaatttatat
    10501 tgttgctaac aaactacctt taatgactcg aaatatcaaa atcagtatag gaaaacaata
    10561 tctagatgat attctaattg tttctgattc tcacagatta atttacacaa caggtcagct
    10621 aaacatcatg aagaagtatc cgcctcgtct gtactatcat ttgaaacatc ttgttgatta
    10681 tcagtttgcg tcactttaga tgttttatta taaattgcgt gtgtcgtata ccttgctaat
    10741 acgaaattta ctgcagctat taaacataag aataaaataa agtaaaaatg atatggaatg
    10801 ttttccatac ttgatatacc aaatatgata gattgtgcta taccattaac aatgtaatac
    10861 attggattca gcattaggat gtgattgata aatacatgat ttggatttgg tatgaaaata
    10921 attggtaaca ataagataca caatacacaa accccataaa atatgatatt tattttttca
    10981 gttaacagtc gaataagacc aaaagtaacg gatattaatc ctacaaaaat agttgccatc
    11041 acaataaaat agaatagcgc tatatatgat gtttcgaagt ttactggttt aaccaatgca
    11101 ctaatcatcg tcaaaatgac tagcataata aaacttaaaa tagacataat aactactggc
    11161 gtcgctgcgg aaatattaaa taacttagtc actaaaaagt cattcatata atgatgataa
    11221 gagatataaa ttgtgaacat caataccgca aaaacaatca atgcaataat tctaaataac
    11281 catcgcacct ctttaacatc ggtcaaatga ctaaaggaaa atagcgtttc acttgcaata
    11341 ataagtatta aacatgcagc taatacgatt agaaaacttt tccaactttg ttttaaacgt
    11401 tctgttacat accttttagt atgcggtaag tttttaaaat ataaaataat attatctagc
    11461 atctttacac ctacaattca atataaatcc atttgtcttc tttcaaattg gcaatacaat
    11521 atccattccc aactttagta atccaaatgt tattgttaat attaaactta tcttttaatt
    11581 cttttttatc tacaattgga taaacaaaac cgtataactg attattatca ttgaaaagta
    11641 attgtatcgg ttgttgtgta acggatgcaa cgaaacggtt atcatcatca ggaactaaag
    11701 tttttataac tgaactatgc tttttatgta attgactgtt aaagtatgca tagtaattac
    11761 tataatttga tttcatatat ggcgctaatt ttttaaaact atgcgtttga tataaacctg
    11821 ccgggttaaa atattctaat ttatctttac taacatagta agtcttatta tcggcggtaa
    11881 cacgataatt tttactattt tcgccagtaa ttgtaactac actatatttt ggaattgtga
    11941 cttgtttatc cccttgcata tccacactgc cattaaaagc aattccataa gctaactttt
    12001 cgccatatgg atctttattt tcattttgca tcgtagcacg attcgtaaaa tctgttatcg
    12061 aaataattcc tatattattg aaaatgagca acatcatcaa tgctaagcct aaaatagtac
    12121 ctgaggctaa agtccaaaaa cgcactaaaa acttaggcgg ttttgcatga ttatagcgct
    12181 caacgcgttt gaaattatag gtcatctctg gtattcttgt acgattcttt ttccaatcta
    12241 aatcaaagtt ttcaatttct tcttttgagt ttaaacttaa tcgatcacgt tcatgttctt
    12301 taaaagatgg aataacttgt ttaagtgacc cttccattct taattgacca tgtgaaaacc
    12361 aagctatata gttactcact tgtgaaattt tatcaatatc atcaccaatt gacacaatcg
    12421 ttaaattatt ttcaatataa tcatttgtta attcaatcgc acgttccata aattgtggtg
    12481 ttaaatagtc aataacatga tttaaaataa taatatttga ttttgatgag cgtgcaatac
    12541 ttaatagtaa ttgagcgtat gccgctttcg aaatatggtt aaccggcttc gttttataat
    12601 cgcctaaatg tgcatattga ataatctgtt cagctttatg atcattaatt tcatatggaa
    12661 atagttgaac taactgcgct gtgtaagctt caacagtttg atgaattaac gattgatctt
    12721 caatatatcc gtagaacaaa tcttcagtac aaactacttt acctttatca ggtttaattg
    12781 cacctgccaa caattgacct accaacgctt tggaagattc aggttcacca attataccta
    12841 atgcttctcc ttgataaata tgtaaactaa tattgtttaa atcgatatct tcagcatcat
    12901 atccaaaagg taaataccat ttcttattct gtttattcct atagtagtgt gttactttta
    12961 gtaactttaa aacaattgaa cttcccatct attttcatcc ttctataatt gtaataacgc
    13021 tataggtaag ccttcttcgg gttcatcgct attaaatcta aacccccacg caaaaatacc
    13081 ttttaatctt tctactttaa aatattgatt actgccatca gtcagtttat aaattttacc
    13141 aatttctatc ttttttctgt ctactaagta actttcggca acaattactt tactttgata
    13201 aacgtcatat tcatttaaaa taccattcat ctcagctttt ctcattttct ctttataaag
    13261 ttgtatttcg tgtcttaatt ctggctcaga catctcactc aatttcttct gttccatcgg
    13321 caataccact ttcttctaat ttagctttaa ttttatcata tttatatcct tttctcataa
    13381 ggccttcgat agtttttgaa attaatttct gttgcgtgta cttctttcga tttttattat
    13441 aaattttttc taaatctcgt tgtaataaat cgtctaaaac cgcttcatct tgtgtaaaat
    13501 ccatttcatt caatacagca tgaatggttc ccatttcaaa ccctttttga attaaagatt
    13561 gcattacttt ttctttaacc ttattttgtg gtcctttttt tgttttagat attttttcag
    13621 caatttggat aatatcatca agttcctgtt gttctctata aagttctgta aacatttcaa
    13681 tgatatttgg ttctatacca agttgataaa gtttttgttg ataaatttta ggtcctttat
    13741 ctgtcgtgcg aatcattgta ttttttaaac tttccgcata atcttgatgg tcgattaact
    13801 tttcgcgata acaatattca atcacttcag aaatcgcttg ctctgatatc tcttcttttt
    13861 gtaaatattg aataacttct ttttcagttc tctttttata tgataaatat tggattgctt
    13921 tgtttaaacc tatgcgataa tgatcatact tttgaatctc tgccatgtca gcagcttcaa
    13981 gttgttgccc tttttttaaa ttaaatttga ctaatgtatc gatatctata cccatttcaa
    14041 attgttcgtc taaaaaaaga ttaaaacgtt ctttgttttt cttttgaact tctattttag
    14101 taatcttcgg cactggatca ccccgttact ttctcaagtt ttatacaata ctcttttatt
    14161 attcaataag ccacttccta tagcaaatgt ttaactttaa atatttttcg atgctaacaa
    14221 aaaatcacac tatcatcttt taaaatgaaa gtgtgattac aagcaaatct gtaaaattta
    14281 ttaagcagaa acaattcaac tttatcatta tgacatttca attaaacctt ctacattata
    14341 gttccaagca tcttacacat gaatgcaagt atttaacgat ttaattgtga catagcctgt
    14401 tgatattgtg tttcattgat ataattttgt tgcttcattt tttctaagtt cgtgcttaca
    14461 cgttgcgtaa aattctctga catattattg atattatata cgctaggtgc attgacttta
    14521 ctagctaaaa tagcgctttg taaaactgtt atgtgagaca ttgttgtact atttttattc
    14581 acggttgttc caaagtaatg gtttgctgcg ccctcaagcg tatattgatt atccccaaag
    14641 taaatattat ttaaataaaa gcttaaaatt tcgttcttat tatattgttt ttcaactcga
    14701 tgagctacaa ataattcttt tacttttcta gtaaatgaac gatcattatc ataaaaataa
    14761 tttttgacaa cttgttgtgt aatggtacta ccaccttgca catctctgtc gctaatcgtt
    14821 gaaaataaag ctctagttgt acctttcaaa tcgaatccat gatgattgta gaatcgttca
    14881 tcttccattg aaataaaggc acctttaaca tactctggca tgttatcagc tgacacaaaa
    14941 ctacttttat tttcaatttt tcttagttca tccacattat cgcgtgtaga taaaaaatac
    15001 atgataccaa taaacaatgc gataatgatt agaatggtta ataatatttt taatagtatt
    15061 cgtttacttt ttttcttttt cggcggtttg ccaactggtt gataatacgt attatagtga
    15121 ggttcgtgtt tcatatgctc aaaatgttca tttgagtttg agtacctatc gcttcttttc
    15181 atgcgtttgc tccttctttt aaaactcact tagtatatac cttgagttta ccagtactat
    15241 cacaaatagg ctacactttt tgggaaaatc agtccaaggg cttacaatcg tatacgccat
    15301 catacttact tttttgtttt ttgaaaagat tatagataaa tcattacaat tttaaatatt
    15361 aatcatgcca aatattgtta tattttgtaa aataaaagac catccctatt aaatgccaat
    15421 agagacgacc ttttatttgt tattcattta ttaaaactaa aatccatatt tcatttcaaa
    15481 cgaaaatata taaattttaa caatcgataa ccacaatact tctattgtaa ttgtttaacg
    15541 atttctcgat taaaatcatc taaatcgtct ggtactcgac ttgttacaat attgttgtct
    15601 acaactactg actcatcaac tacatgtgcg cctgcatttg ataaatcttt gcgcacgttc
    15661 aatactgctg ttaacgtacg accttttaaa tcatctgtat ctattagtat ttgtggccca
    15721 tgacaaatgg caaatgttgg tacatcattt ttagtaaagt atttagcaaa tgtgccatat
    15781 cgaccttctg tatctccacg taaatgatct ggtgaaaatc ctccaggaat taataatgca
    15841 tcataatctt ctggtttagc ttctgcaatg cctacatcga cagtaacttt ttcaccgtgt
    15901 ttaccaacaa cttcactatt tgcagtatcc ccaatcacta cagtattaaa tccagcattc
    15961 tctaatgcct ctttaggact tgaatatttt atatcttcaa attcgtttgc tagaataatt
    16021 gctacttttt tagtcattga aaatcacatt tctatatatc attgatataa ttactataga
    16081 caagtaaatc agtgattaaa catacaagat ataaaaaata ttaagcgact atcgcgatat
    16141 ctaactctaa cacatcttat gtggcattta cttagatact aatttaacct tttcttcaag
    16201 ctgatctaac aatccaatcc attcatctat atcttcaaca cgtacttcat caggatttac
    16261 atgatcgata tcctcaataa acttatttaa acgtgctttt atctgttcga ttgtttgctg
    16321 ttcattcata aaaagttaac tccttttatt ttgttttctt tttcattatt atcctaacag
    16381 aaattgcgtt aaagcgatat aatcttagct atatttatga cattcaaatt attttgactt
    16441 ttaaaaatcc ccttttcaat taactaaaat taagagataa tttgttacga gtgataatac
    16501 gaagtggtat cataccgata tgaaccaaat agaaagaagg aagtttaaga cgatgaatag
    16561 cgtcaaattg aagcaacctg ttagcattta caatgatcca tgggaatcat ataacgatgt
    16621 taaagaacat ggccaattaa ctttaagtaa catcgaattt acaactacaa atctttgtaa
    16681 tatgcgttgt agccactgtg cagttggtta tactttacaa actgtcgacc ccgagccttt
    16741 agatatggac ttaatttatc gtagacttga tgaaattcca aatctgcgaa cgatgtcaat
    16801 tacaggtggc gaaccaatgt tttctaaaaa gtctattaga aatgttgtta aacctctatt
    16861 aaagtatgca catcatcgag gtatatatac acaaatgaat tcaaacctaa cattgcctca
    16921 agatcgttat ttagatattg ctgaatatat cgatgttatg catatctcac ataactgggg
    16981 aacaactgat gaattcgcaa atgttggctt tggcgcaatg aagaagcaac caccgttaaa
    17041 agctaagtta aaattatatg aacaaatgat ttcgaatgca cgtacattat cagaacaagg
    17101 aatgtttgta tctgcggaaa caatgctcaa tcaaagtacg ctaccacatt tacgaaaaat
    17161 acatcaagaa gtcgttcatg atatgaaatg tagcagacac gagattcacc ctatgtatcc
    17221 agctgacttt gcaagtcaat taaatgtgtt aactctagcg gaaatgaaaa agacaattca
    17281 tgatatattg gatttcagag atgaagatat ttggatgtta tttggtactt tgcctgtgtt
    17341 tccatgctta aaggatgatg aagatcaaaa gttactatca cgtttaagaa atgctaacaa
    17401 tgtaacgact agaaatgacc cggatggccg tagtcgttta aatgtcaatg tatttacagg
    17461 taatgtaatc gtaactgatt tcggagatga aacaggtaca atttcgaata tacaaaaaga
    17521 taaattaaca gatgtatttg ataaatggtt atcctctgat cttgctaaat cattaaattg
    17581 tcattgttcc gagtttagtt gtttaggacc aaatgttctt gttaaaaata tgtactatcc
    17641 gaatatggat tttaaagata atgagcgtca tatgcacaaa caaccacaaa ttatacaatt
    17701 ttaaaaactc ttaattatgc ggagaagcac tttatcgata agtagtctcc gcatatttta
    17761 atgctattat aaaataaaaa acaattaatt gctggcagta ctctacttta a
//
LOCUS       DAOBBH010000039        16822 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.039, whole genome shotgun sequence.
ACCESSION   DAOBBH010000039 DAOBBH010000000
VERSION     DAOBBH010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16822
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.039"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..956)
                     /gene="csbB"
                     /locus_tag="P6L01_000964"
     CDS             complement(<1..956)
                     /gene="csbB"
                     /locus_tag="P6L01_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosylation protein
                     CsbB"
                     /protein_id="HDP3030048.1"
                     /translation="MKIRVVIPCFNEGEVITQTHQQLTEILSQDSSVKGYDYNMLFID
                     DGSTDTTIDEMQHLATIDRHVSFISFSRNFGKEAAMIAGYQHSTEFDAVIMIDCDLQH
                     PPEYIPKMVEGFMEGYDQVIAKRDRSGENFSRKTLSHLYYKLVNCFVEEVQFDDGVGD
                     FRLLSQRAVKSIASLEEYNRFSKGLFEWIGYNTKVFTYQNVERQKGESKWSFKKLFNY
                     GIDGLISFNSKPLRMMIYLGLFIFSISVLYIIYLFINIMISGVNIPGYFSTIAAILLL
                     GGIQLISIGVVGEYIGRIYYEVKARPKYIIQATNLSSIENDE"
     gene            complement(1088..1927)
                     /locus_tag="P6L01_000965"
     CDS             complement(1088..1927)
                     /locus_tag="P6L01_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDP3030049.1"
                     /translation="MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAF
                     DTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYL
                     DLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
                     QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDNPVIVKIAEKYHKTPAQVVL
                     RWHLAHNRIIIPKSQTPKRIQENIDILDFNLELTEVAEIDALDRNARQGKNPDDVKIG
                     DLK"
     gene            complement(2149..3498)
                     /locus_tag="P6L01_000966"
     CDS             complement(2149..3498)
                     /locus_tag="P6L01_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDP3030050.1"
                     /translation="METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEG
                     NKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALT
                     TTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMN
                     GSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHA
                     KDIMVPRTQMITLNEPFNVDELLETIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEY
                     ASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDIL
                     EEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGW
                     LQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVILNYEFNEARPTIGQSDEDEKSE"
     gene            complement(3719..4900)
                     /gene="nagA"
                     /locus_tag="P6L01_000967"
     CDS             complement(3719..4900)
                     /gene="nagA"
                     /locus_tag="P6L01_000967"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDP3030051.1"
                     /translation="MSELIIYNGKVYTEDGKIDNGYIHVKDGQIVAIGEGDDKAAIDN
                     DTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLA
                     TTMTQSTDKIDKALTNIAKYEAEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRP
                     FIDKIKHFQETANRLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAVE
                     RGAKHVTHLYNAATPFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGN
                     ERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLANGALAGSILKMNHGLRNLISF
                     TGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTF
                     S"
     gene            complement(5208..7166)
                     /locus_tag="P6L01_000968"
     CDS             complement(5208..7166)
                     /locus_tag="P6L01_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDP3030052.1"
                     /translation="MRVTELLTKDTIAMDLMANDKNGVIDELVNQLDKAGKLSDVASF
                     KEAIHNRESQSTTGIGEGIAIPHAKVAAVKSPAIAFGKSKEGVDYQSLDMQPAHLFFM
                     IAAPEGGAQTHLDALAKLSGILMDENVREKLLHASSPEEVLAIIDEADDEVTKEEEAE
                     AEAQQVATAEQSSKQSNEPYVLAVTACPTGIAHTYMARDALKKQADKMGIKIKVETNG
                     SSGIKNHLTEQDIENATGIIVAADVHVETDRFDGKNVVEVPVADGIKRPEELINKALD
                     TSRKPFVARDGQRKGNSNDSQEKLSPGKAFYKHLMNGVSNMLPLVISGGILMAIVFLF
                     GANSFNPKSSEYNAFAEQLWNIGSKSAFALIIPILSGFIARSIADKPGFASGLVGGML
                     AISGGSGFIGGIIAGFLAGYLTQGVKAMTRKLPQALEGLKPTLIYPLLTVTATGLLMI
                     YVFNPPASWLNHLLLDGLNNLSGSNIVLLGLVIGAMMAIDMGGPFNKAAYVFATGALI
                     EGNAAPITAAMIGGMIPPLAIATAMLIFRRKFTKEQRGSIIPNYVMGMSFITEGAIPF
                     AAADPLRVIPSMMIGSGIGGAIALGLGSRITAPHGGIIVIVGTDGAHLLQTLIALLVG
                     TLVSALIYGLIKPKLTETEIEASKSMDE"
     gene            complement(7172..8092)
                     /gene="pfkB"
                     /locus_tag="P6L01_000969"
     CDS             complement(7172..8092)
                     /gene="pfkB"
                     /locus_tag="P6L01_000969"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDP3030053.1"
                     /translation="MIYTVTFNPSIDYVIFTNDFKNDGLNRATATYKFAGGKGINVSR
                     VLKTLDVESTALGFAGGFPGKFIIDTLNNSAIQSNFIEVDEDTRINVKLKTGQETEIN
                     APGPHITSTQFEQLLQQIKNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
                     DAEKELAESVLPFHPLFIKPNKDELEVMFNTTVNSDTDVIKYGRLLVDKGAQSVIVSL
                     GGDGAIYIDKEISIKAVNPQGKVVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACG
                     TATAFDEDLATRDAIEKIKSQVTISVLDGE"
     gene            complement(8089..8853)
                     /locus_tag="P6L01_000970"
     CDS             complement(8089..8853)
                     /locus_tag="P6L01_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDP3030054.1"
                     /translation="MMIITEKRHELILEELSHKDFLTLQELIDRTGCSASTIRRDLSK
                     LQQLGKLQRVHGGAMLKENRMIEANLTEKLATNLDEKKMIAKIAANQINDNECLFIDA
                     GSSTLELIKYIQAKDIIVVTNGLTHVEALLKKGIKTIMLGGQVKENTLATIGSSAMEI
                     LRRYCFDKAFIGMNGLDIELGLTTPDEQEALVKQTAMSLANQSFVLIDHSKFNKVYFA
                     RVPLLESTTIITSEKALNQESLKEYQQKYHFIGGTL"
     gene            complement(9103..9585)
                     /gene="ybaK"
                     /locus_tag="P6L01_000971"
     CDS             complement(9103..9585)
                     /gene="ybaK"
                     /locus_tag="P6L01_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDP3030055.1"
                     /translation="MAKNKKTNAMRMLDRAKIKYEVHSFEVPEEHLSGAEVAELIQAN
                     VKTVFKTLVLENTKHEHFVFVIPVSETLDMKKAAALVGEKKLQLMPLDNLKNVTGYIR
                     GGCSPVGMKTLFPTVVDKSCENYSHISVSGGLRTMQITIAVEDLITITKGKIGAVIHE
                     "
     gene            complement(9759..10211)
                     /locus_tag="P6L01_000972"
     CDS             complement(9759..10211)
                     /locus_tag="P6L01_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030056.1"
                     /translation="MTVKNLFLGFVAVILTVCLIGLLILATNEDALAKVHKTINTLNA
                     INVSTEDTYKKKMDILNIHTAKASEVNENVEKQNHFKHRVNANKSNSFNEQECQVIAD
                     RYADKHINDNYGLERISKTNHGYNYVYSNDNSTSKQHVSISNQGIITK"
     gene            complement(10504..11670)
                     /gene="norA"
                     /locus_tag="P6L01_000973"
     CDS             complement(10504..11670)
                     /gene="norA"
                     /locus_tag="P6L01_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorA"
                     /protein_id="HDP3030057.1"
                     /translation="MNKQIFVLYFNIFLIFLGIGLVIPVLPVYLKDLGLTGSDLGLLV
                     AAFALSQMIISPFGGTLADKLGKKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGG
                     MSAGMVMPGVTGLIADISPSHQKAKNFGYMSAIINSGFILGPGIGGFMVEVSHRMPFY
                     FAGALGILAFIMSIVLIHDPKKSTTSGFQKLEPQLLTKINWKVFITPVILTLVLSFGL
                     SAFETLYSLYTADKVNYSPKDISIAITGGGIFGALFQIYFFDKFMKYFSELTFIAWSL
                     LYSVVVLILLVFANGYWSIMLISFVVFIGFDMIRPAITNYFSNIAGERQGFAGGLNST
                     FTSMGNFIGPLIAGALFDVHIEAPIYMAIGVSLAGVVIVLIEKQHRAKLKEQNM"
     gene            11879..12301
                     /locus_tag="P6L01_000974"
     CDS             11879..12301
                     /locus_tag="P6L01_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDP3030058.1"
                     /translation="MKLQKSNHTILLVLEKGEDIVECITTFADDQDLTFTSVSGIGAC
                     DDVVLKFFNLTTKQYEEKHITEPLELTSLLGNISRLDNGHFAHLHATFGTQSYETFSG
                     HLAKAIVSATAEIILTVTDLDIQRSFKDAVGLNLLDPQ"
     gene            12487..13104
                     /locus_tag="P6L01_000975"
     CDS             12487..13104
                     /locus_tag="P6L01_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HDP3030059.1"
                     /translation="MQSRYIARIYFLILFIVSLFETKIFQFMTLNLFLAYIPFELSLL
                     LRLFKPNKKYEWPLFIIYSFIFVLLLPNTFYMVTDLIHLNQFQFNFYAGLNLSEWKYF
                     TYLLLGVFLAVYVMILIYFEILTLTSHRWLNRLIVVVLMFLNGFGIYIGRFLRLHSVY
                     FFNEPLHVLNHILESLTLKTAMFVCFMVVMQAAILLFGKGVRLNK"
     gene            13101..13385
                     /locus_tag="P6L01_000976"
     CDS             13101..13385
                     /locus_tag="P6L01_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030060.1"
                     /translation="MTINIIVLIVSIIVFQLIIGHIWHDIGLSYLRSILLMMLPFGLG
                     VFIQQVSYYERQYPKWQVPQNIKVRLKYIYLATFLEYVVLYLTLFTDILR"
     gene            complement(13540..14913)
                     /locus_tag="P6L01_000977"
     CDS             complement(13540..14913)
                     /locus_tag="P6L01_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDP3030061.1"
                     /translation="MAIAVLLNRMFRMEHNPLFEYIYQQKEDIDACYFIIPEEDMSSA
                     SDLKAQFYRGTLQRFYQSLHAEKLTPYVMSYDDIISFCKENNISEVVIAGDIMSYHLE
                     EYDILHQRSLFNEARIAVTLIRGNHYFKASKTMNQQGEPYKVFTSFYRKWRPYLRHRD
                     VYHYDLKSFEDFVIASPDDLVFDDIAFGSSQVIEQNKWQHFLDQDIQNYESGRDYLPE
                     VLTSQLSVALAYGLLDIIEIFNDLLARYDEDEANHEAFIRELIFREFYYVLMTQYPET
                     SYQAFKPKYRQIKWSQNEANFNAWCEGQTGFPIIDAAIMELTQTGFMHNRMRMVVSQF
                     LTKDLFIDWTWGEKFFRKHLIDYDAASNIHGWQWSASTGTDAVPYFRMFNPIRQSERF
                     DAKALYIKTYLPIFNQIDAKYLHDTQRNESNLFEQGIELGSHYPRQMVDHQEKRTQVL
                     ATFKALD"
     gene            complement(14999..16552)
                     /locus_tag="P6L01_000978"
     CDS             complement(14999..16552)
                     /locus_tag="P6L01_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HDP3030062.1"
                     /translation="MSEEKHVVEHEQQKKEKTKKQYKPFWIVMSFIILIVVLLLPAPS
                     SLPIMAKAVLAILAFAVIMWVTEAVSYPVSATLIIGLMILLLGFSPVQNLGEKLGNPK
                     SGSAILAGSDLLGTNHALSLAFSGYATSAVALVAAALFLAAAMQETNLHKRLALLVLS
                     IVGNKTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLI
                     ITSVQAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSVALYF
                     IMIKVMPPEINTIEGGKDLIKEELHKLGPVSPREWRLIVISMLLLLFWSTEKVLHPID
                     SASITIIALGVMLMPKIGVMTWKHVENKIPWGTIIVFGVGISLGNVLLKTGAAQWLSD
                     QTFGVLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLHLGDQS
                     IGFVLIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKAGIPLTIVGYILVIVFSM
                     TYWKWLGLL"
BASE COUNT         5435 a         3193 c         2303 g         5891 t
ORIGIN      
        1 ttctcatcat tttcaatact tgaaagattt gtagcttgaa taatatattt aggacgtgcc
       61 ttaacttcat aatatatcct gccaatatat tcacctacaa caccaattga aattaactgt
      121 atgccgccta ataataaaat agctgcaatc gttgaaaaat atcctggaat attaacacca
      181 gatatcataa tattgatgaa taaatagata atatatagca cgcttattga aaagataaac
      241 aagccaagat aaatcatcat tctcaaaggt ttactattaa aggaaatcaa tccatcaata
      301 ccataattaa ataacttttt aaaggaccac ttagattccc ctttttgtct ctcaacattt
      361 tgatacgtaa acactttagt attatagcct atccattcaa ataacccttt tgaaaatcga
      421 ttatattctt caagtgatgc aatggattta acagctcttt ggcttaaaag tctaaaatca
      481 ccaacaccat catcaaattg tacttcttct acaaagcaat taactaactt ataatacaaa
      541 tggcttaatg ttttgcgact aaaattttca ccacttctat cacgctttgc aatcacttga
      601 tcatagcctt ccataaaacc ttcaaccatt ttcggaatat attcaggtgg atgttgcaaa
      661 tcacaatcta tcatgatgac tgcatcaaat tcagtactat gctggtaacc tgcaatcata
      721 gctgcttctt ttccaaaatt tctactaaaa gaaataaagc tgacatgcct atctattgtg
      781 gcaagatgtt gcatttcatc tatagtggta tccgtacttc catcatctat gaaaagcata
      841 ttataatcat agcctttcac actactatct tgtgaaagta tttcagttaa ttgttgatgt
      901 gtttgtgtaa tgacttcccc ttcattaaaa caaggaatga cgactctaat tttcatctat
      961 gtccatcctt tttatttaaa agattttgaa tgtacctgag tattgtttta tcaaatagta
     1021 aaaaccaact catttatttt aatgtacaca ttaaaaggca ttcataaacg taaaatttaa
     1081 catccagtta ttttaaatcc ccaattttca catcatctgg atttttacct tgtcttgcat
     1141 ttctatctaa agcatcaatt tcagctactt ctgttaattc taaattaaaa tctaaaatat
     1201 ctatattttc ttgaatgcgt ttaggtgtct gagattttgg aataataatt ctattgtgtg
     1261 ctaaatgcca acgtaataca acttgtgctg gtgttttatg atatttttca gcaattttaa
     1321 caatgacagg gttgtctagt agtcctctat ttctcatcaa aggcatccat gcagtcactt
     1381 taatatcgtg acgatcacaa aattcttgta attcttgttg gttaaaatat ggatgtacct
     1441 caatttgatt caccattggt ttgatacttg attgagccat taatttttct agatgatgaa
     1501 cattaaaatt acatacacct attgctttta ccttaccttg ctcgtaaagt tcttccatag
     1561 ctttatatgt ttctaaaaat agaccatctg cttcacaagg ccaatgtatt agaaataaat
     1621 caagataatc agtttgtaaa ttttcaatcg atttgttgaa atattcgaat gttttctcat
     1681 aaccttgata gtcattccat aacttcgttg ttataaacaa atcttctcta tcgacgccat
     1741 tatcctttaa tgctcgtcct agtgaagcct cattatcata aaagtatgct gtatcaaacg
     1801 ctctatagcc tgcgtcaatt gcagcattta caactttagt catatcttcg tcagagattt
     1861 tataaacacc taacccaact gaaggcatcg ggtatccatt atttaatatt tgtatctcat
     1921 tcaacatctt tatatctctc caatctatgt atcttttata tctttacatt accctaaatt
     1981 tttcaacaaa ctcaattaat gcgaattatc gctttcaata aaaaatattc attaaatcat
     2041 taaagatatt gagttccaat actattttca cttttcatta attttagtca aaaaaataac
     2101 caaccaaaaa tgaattaaat cattcttagt tggtttatat attaatatct attctgattt
     2161 ttcatcttca tcagactgtc cgatagtagg tctcgcttca ttaaattcat agtttaatat
     2221 cacccaaata atttggtggt tatcgatttc tgaaacaacc cagcgatcat aagttgtatc
     2281 cacgtaatca tctttttgta aattggtatt acgagattgt aaccatccac ctatcgtatc
     2341 aatatcctca gagtcatcaa attctatacc gaactcttca gttaaatcat ccaatagtac
     2401 tctgccattt acttggaatg tcttattatc aattttaacg atatcattca cttcatcatc
     2461 atcaaattca tcacgaattt ctccaacgat ttcttctaaa atatcttcca tcgttaaaat
     2521 acctgccgtt ccaccatatt catctataat aagactcata tgtacatgtt cacgttgcat
     2581 tctaattaat gcatcactga tacgtgttgt ctctgaaatc attggcaact catgtatata
     2641 gtttgctatt ttaatcgttt ttccagaagc gtattcagtt aaaaattctt tgacgttaat
     2701 aaatccttta atgtggtctt tatcaccatc atcagtaatt ggataacgcg taaattgatg
     2761 ttcttttatt gtttctagta attcgtctac attaaaaggt tcatttagtg taatcatttg
     2821 agttctaggt accattatat cttttgcatg tctttcatcg aatgaaaaga tattttgcat
     2881 atatgccaat tcagtttggt tgatttctcc accattataa ctattgttaa taataatttt
     2941 gatttcttct tctgacattg catcagtttg ggcatcagga tttacaccaa acattctaat
     3001 aataacacgt gcagaaccat tcatcagcca aatcaatggt ttcataatgt taccgaaata
     3061 gaacaatggt cttgcatata ctaaagcaag cttttcagta tgttgaatcg ctatagattt
     3121 aggcgctaat tcaccaagta ctacatgcaa atacgtaacg attataaatg acactgcaaa
     3181 cgaaatcgtc gtcgttaatg cagttggtaa attgattgct tcaaatatcg ggtgtaatag
     3241 cttttcaaac gttggttcac caagccaacc taaccctaaa gatgttactg ttatacctaa
     3301 ctgacaagca gaaagataat aatctagatt agcaatcatc ttttttacta ttttagcagg
     3361 tttatttcct tcatctgcta gctgttcaat tcttgttgct ctaattttta ctaatgcaaa
     3421 ttctgaacca acaaatacag tggttaatgc aattaataga ataaatataa tcaaactaat
     3481 tatggtcgaa gtttccaatt aattccctat ttctagggat tcacctccat gttatatgtg
     3541 tcactcatgg gtaacacgca ttcaaattta tcactatgac ttaaattaca actacatgtt
     3601 atcgccttcc catttagaca cccccagaaa aaaatgttat tgcttctatt ttatcataat
     3661 ataaagtgct tatgttaaca gattaaatct attgcataca tttaattatg attatttatt
     3721 agctaaatgt gtgaaccttg ccttgtttta ttgtagattt tacattcata tcatcatcta
     3781 gaataacaag atctgcatcc ttatttactt taatactacc ttttctatca tcgataccta
     3841 atgcaatggc ttgatttaaa cttgttactc gccataaatg atctaatgta tcacctgtaa
     3901 atgatattaa gttacgtaac ccatgattca tttttaaaat actaccagca agcgcaccat
     3961 ttgcaagacg tgcttgttgc gattgaacag ttactttttg tccacccaaa tcatattctc
     4021 cttcaggcat accttttgca cgcattgcat cggtaattaa ataaaaacgt tcattacctt
     4081 tcatacggta agcaattgca accgatgccg gatgagaatg tgtgccatca acaatcattt
     4141 cggtatgtag agcatcattc aaccatgctg ctccaaaaac acctggttct ctatgttgga
     4201 atggcgtcgc tgcattatat aaatgcgtga catgtttagc tcctcgctca acagcttcga
     4261 ctgcttcttc gtatgttgcc actgtatgac caattgaaaa aataatgtca tctttatacg
     4321 tttcaagcgc ttcttttgca ccttcaactt caggtgcaaa cgtcataatc tttattaatc
     4381 tgttagcagt ctcttgaaaa tgtttaattt tatcgataaa tgggcgtaca acgtattgcg
     4441 gatgttgagc accaacttta ttttcagata taaatggtcc ttctaaatgt atacctacaa
     4501 tttccgctgc attgtgaaca tcttgctccg cttcatattt agcaatattt gtaagtgctt
     4561 tatctatttt atcagtagat tgcgtcattg tagtggccaa gtatgatgtc gtcccttcag
     4621 ataacaaatt ttcggataga tattttaagc catcgtatga cccatccatt gcatcttgac
     4681 cataaccacc atgaatatgt atatcaataa aacctggtaa tacatgatga cctttagcat
     4741 caatcacttg aattttattt gtcgtatcat tatcaattgc tgctttatca tccccttctc
     4801 caattgcaac aatctgtcca tctttcacat gaatgtaacc attatcgatt ttgccatctt
     4861 cagtataaac tttgccgtta tatataatta attctgacac gtataaaccc ttctttattt
     4921 ataaattttc actttaagac actcatattt tactatatct gcttcgtttg gcataacttt
     4981 ttgcaaaata ttaaaaaagt ctatacattt gtggtgtcta tttcttcatt catttatcta
     5041 aaaataaaaa caccttcata tcaattcata aaaactgaat taatagaaag tgtttttaca
     5101 gctttgtata agctaccgtt aacacgatgt ataaaactaa catatattca ttgaacttac
     5161 aacttaatat gaagctcttt gctaacaatc attttacatc attaaaacta ctcgtccatt
     5221 gattttgaag cttcgatttc tgtttcagtt aactttggtt taataagtcc ataaattaat
     5281 gctgaaacta atgtaccaac tagaagtgca ataagagttt gaagtaagtg tgcaccatca
     5341 gtaccaacaa ttacaataat accaccatgt ggcgcagtaa ttcgagaacc taagcctaaa
     5401 gcaattgcgc cacctatacc tgaaccaatc atcattgaag gaataacacg taatggatcg
     5461 gcagctgcaa atggaatcgc accttctgta ataaaggaca tacccatcac atagttaggg
     5521 ataattgaac cacgttgttc ttttgtaaat ttacgtctga aaattaacat cgctgtcgca
     5581 attgctaacg gtggaatcat accaccaatc attgcagctg taattggtgc tgcattacct
     5641 tcaatcaacg cacctgttgc aaaaacatat gccgctttgt tgaatggacc gcccatatca
     5701 atcgccatca tagcgccaat aactaaacct aataatacaa tattagaacc tgataaattg
     5761 tttaatccat ctaataacaa atgatttaac caagatgctg gtggattaaa gacataaatc
     5821 atcaataagc ctgtagctgt cactgttaat agtggataaa ttaatgttgg ctttaatccc
     5881 tctaatgctt gtggtaactt acgtgtcatg gctttaacac cttgtgttaa gtaacctgct
     5941 aagaaacctg caataatacc accaataaat cctgaaccac ctgaaattgc taacatacca
     6001 cctacaagac ctgaagcgaa accaggttta tccgcaatac tacgtgcaat gaatccagat
     6061 aaaattggaa tgattagcgc gaatgcactt ttactaccaa tgttccaaag ctgctctgca
     6121 aacgcattgt actctgagct ttttggatta aatgaatttg ctccaaataa aaatacaatt
     6181 gccattaaaa taccaccaga tattacaagt ggcaacatgt tagaaacacc gttcattaag
     6241 tgtttataga atgctttacc tgggcttaat ttttcttgac tgtcatttga gttacctttt
     6301 ctttgaccat cacgggcaac aaaaggttta cgacttgtat ctaatgcttt attaattaat
     6361 tcttctgggc gtttaatacc atctgctact ggtacttcta cgacattttt accatcgaag
     6421 cgatccgtct caacatgaac atcagcagca acaatgatac ctgttgcatt ttcaatatct
     6481 tgttcagtta aatggttttt aatgccgctt gaaccgttcg tttctacttt aattttaata
     6541 cccatcttat ccgcttgttt tttcaatgcg tcacgtgcca tgtacgtatg tgcaatacct
     6601 gttggacaag cagttactgc taacacatat ggctcgttag attgtttaga tgattgttct
     6661 gcagttgcaa cttgttgtgc ttcagcttct gcctcttctt cttttgtcac ttcatcatca
     6721 gcctcatcta taatcgctag tacttcttca ggtgatgaag catgtaataa tttctcgcgt
     6781 acattttcat ccattaaaat accagacaac ttagctaaag catctagatg tgtttgggcg
     6841 ccaccttctg gcgctgcaat cataaagaat aaatgagctg gttgcatatc taaactttgg
     6901 taatctacac cttctttaga tttaccgaat gcgatagctg gagacttaac tgcggctact
     6961 ttcgcatgtg gaatagcaat accttcgccg ataccagttg tactttgtga ttctcgattg
     7021 tgaatcgctt ccttaaatga cgcgacatca cttaatttac ctgctttgtc taattgattt
     7081 actaactcat caataacacc atttttgtca tttgccatta aatccattgc tatcgtatct
     7141 tttgttaata actctgttac tctcattatt ttcactcccc atcaagtacg ctaatcgtaa
     7201 cttgtgattt tattttttct atagcgtccc gtgttgctaa gtcctcatca aatgccgtgg
     7261 cagtaccgca tgcgactgct tgttggaatg ctttttcaat cgttaaacct gaagcaattc
     7321 cagccaccat gcctgcaact gtactatcac cagagccaac tgtattaacc actttccctt
     7381 gtggattaac tgctttaata ctgatttctt tatcaatata aatagcacca tcaccgccaa
     7441 gcgagacaat aacagattgc gcacctttat caactaacaa acgaccatat ttaataacat
     7501 ctgtgtctga gttcactgtt gtattaaaca tcacttctaa ttcatcttta ttaggtttaa
     7561 taaatagtgg atgaaatggt aaaacgcttt cagccaattc tttttcagcg tcgactacta
     7621 atttagcacc tgtctgtgct gtaatttgtg caatttgcgc atacgcatcg cttggaatac
     7681 tacttggtac acttccagca acaataacta tatcttcgct tgttgtattt ttaatttgtt
     7741 gtaacagttg ttcaaattgt gttgacgtta tatgaggacc cggtgcattg atttctgttt
     7801 cttgtcctgt ttttaatttc acattaatac gtgtatcttc atcaacttca ataaaattcg
     7861 attgaattgc actgttattt aatgtatcta taatgaattt cccaggaaat ccacctgcaa
     7921 atcccaaggc agttgactca acatctaatg tctttaagac gcgcgagaca ttaatacctt
     7981 tccccccagc gaatttatat gttgctgttg ctctgttcaa accatcattt ttaaaatcat
     8041 tcgtaaaaat gacatagtca attgaaggat tgaaagtcac tgtataaatc ataaagtccc
     8101 tcctataaag tgatactttt gttggtattc ttttaacgat tcttgattta atgctttttc
     8161 agatgtgatg attgtcgtac tttctagcaa aggtacacga gcaaaatata ctttattaaa
     8221 cttagaatga tctataagta caaatgattg attggctaat gacattgctg tttgtttaac
     8281 taatgcctct tgctcatcgg gagtagttaa tccaagttca atatctaatc cattcatccc
     8341 gataaaagct ttatcgaaac aatatcgtct taatatctcc atagcactag aaccaatcgt
     8401 agcaagtgta ttttctttaa cttgaccacc tagcataatt gttttaatac cttttttaag
     8461 taaagcttct acatgtgtta aaccattggt taccacaatg atatctttcg cttgaatata
     8521 tttaattagc tccaatgtag atgaaccagc atcgataaat aagcattcat tatcgttgat
     8581 ttgattagct gctattttag caatcatttt cttttcatca agattcgttg ctaatttttc
     8641 agttaaattc gcctcaatca tacgattttc ttttaacatt gcaccaccat gcacacgttg
     8701 caatttccct aattgttgta gtttagataa atctcttcgt attgttgaag cactgcaacc
     8761 agttcgatct attaattctt gtaaagtcaa aaaatctttg tgcgaaagtt cttctaatat
     8821 taactcgtgt cttttttcag taattatcat catcacccca tctcactttg tattataatg
     8881 agtacgcttt cacttttcaa tcataaatta ccaaaaacaa tcacaaacac ccaaattgtg
     8941 acgataaaat tacattttta tgttcaaata ctcaattata atgactgttt atgatcgtaa
     9001 aatgctaatt caatcaaaaa ggagtgtggc agaaatgaat tttataaaaa tttcatctct
     9061 gccacactcc taatcttgcc ccatactttt tgttgttatt aatcattcat ggataactgc
     9121 tccaattttg cctttagtta ttgtaatcaa atcctcaaca gctattgtga tttgcattgt
     9181 tcgaagccca ccactcacac tgatatgact ataattttca cacgatttgt caacgactgt
     9241 tggaaacaat gttttcatac caacaggcga acacccacca cgaatgtatc ccgttacatt
     9301 tttcaaatta tctaaaggca taagctgcaa tttcttctct ccaaccaaag cagctgcctt
     9361 tttcatatct aaagtttcac ttactgggat aacaaataca aaatgttcat gttttgtatt
     9421 ttctagaaca agcgttttaa atactgtttt aacatttgct tgtataagtt ctgcgacttc
     9481 tgcacccgat aaatgttctt ctggtacctc aaagctatga acttcgtatt taatttttgc
     9541 acgatcaagc atacgcatcg cgttcgtttt cttattttta gccatttcaa cacctcattt
     9601 atacttcttc agaactatat taacatttta aaatacatta cggattggtt gattttgaca
     9661 taacatttac atcattttat aagttgattt caccttttta aattaaatta ttgcaaaata
     9721 aaaacagtat atccaattag aatacactgt tccatctatt atttcgttat tatgccttga
     9781 tttgaaatac ttacatgttg cttactagtt gaattatcat tggaataaac ataattatat
     9841 ccatgatttg tcttagaaat tctttctaaa ccataattat cattgatatg cttatctgca
     9901 taacgatcag caataacttg gcactcttgt tcgttaaaag aatttgattt atttgcattc
     9961 acacgatgtt taaaatgatt ttgcttttcc acattttcat tcacttcaga tgctttagca
    10021 gtatgaatat tgagaatatc cattttcttt ttataagtat cttcagttga tacatttatc
    10081 gcgttaagcg tattaattgt tttatgtacc ttagcaagcg catcttcatt tgttgctaat
    10141 attaataaac caattaaaca aacggttaat attacagcaa caaagcctaa aaataaattt
    10201 tttactgtca tccccttacc cacatttcct tactcatttt ttatcatctt aacacattaa
    10261 cgttaatgaa ttaggtatgt ggattgcaat ttaaaaagaa tgtaatattt gtaacatgtt
    10321 tattactcaa aatcattttt tgttacatat aacatttaaa tataaagagt aaaacattta
    10381 gtcaatcgaa tctttttaaa tgtaaagcat cgaattttct taataaaatt cataagaaaa
    10441 acgatgctaa tcattcataa tattacgctt tactttatat caatatacac caaaatactt
    10501 atgctacata ttttgttctt tcaattttgc tctatgttgc ttttcaatta aaacaataac
    10561 aacacctgct aatgaaacac ctatagccat ataaattggt gcttcaatgt gtacatcaaa
    10621 taacgcacct gcgattaaag gacctatgaa attacccata ctagtgaatg tcgagttcaa
    10681 tccgcctgca aagccttgcc tttctccagc aatattagaa aaataatttg taatggctgg
    10741 tcgtatcata tcaaaaccta tgaagacaac aaaactgatt aacattattg accaatagcc
    10801 attagcaaaa actaataata ttaagacaac aactgaatat aataatgacc aagctataaa
    10861 tgttaactct gagaaatact tcataaattt atcgaagaaa tagatttgga aaagtgcccc
    10921 aaatataccg ccacccgtaa tagcaatcga aatatcttta ggtgaataat ttaccttgtc
    10981 agctgtgtat agtgaataca atgtttcaaa tgcagataaa ccaaacgata atacaagtgt
    11041 taaaataact ggtgtaataa acactttcca gttaattttc gttagcaatt gtggctctaa
    11101 cttttggaaa ccacttgtcg tagacttttt cggatcgtga atcaatacaa ttgacattat
    11161 aaatgctaga atacctaatg ctcctgcaaa gtaaaatggc atacgatgtg aaacttctac
    11221 cataaatcca ccaatccctg gtcctaaaat gaatccagaa ttgataatcg ctgacatgta
    11281 gccaaagttt tttgcttttt gatggcttgg tgaaatgtca gctattaaac ctgtcacacc
    11341 aggcattacc ataccagcac tcataccacc aatcactctc gataacatca ataccgaaaa
    11401 attgtggcca actgcaaaca taaattctga cactgaaaac aaaattaatc ctatacatat
    11461 aattaatttc ttccctaatt tgtcagctag cgtaccacca aacggcgata taatcatttg
    11521 agataacgca aaagcagcaa ctagtaatcc taaatcacta ccagttaatc ccaaatcttt
    11581 taaataaaca ggcaagactg gtattactaa accgatacct aaaaaaatca agaaaatatt
    11641 aaaatataag acaaaaatct gtttattcat atgctcacct cttttttctc catgtcatgc
    11701 ttaaagctgt tattcttctt ttccacgaca gattgcaatt catgctaaat attcgtaaaa
    11761 agtttctata ttgtattaca acattgctac atttgacaat attttttctt tgtaattatc
    11821 actattttcc atttaattgt ataattaatg acatattaaa ataaagggga ttgatcatat
    11881 gaaattgcaa aaaagtaatc atactatact actagtgttg gaaaaaggag aagatatcgt
    11941 tgaatgtata acaacctttg cagatgacca agacttaaca tttacatctg tcagcggcat
    12001 tggtgcatgt gatgacgttg tattaaaatt tttcaattta acaacgaaac aatacgaaga
    12061 aaaacatatt actgagccac tggaattgac gagcttatta ggtaatattt ctcgtctaga
    12121 caatggtcat tttgcacatt tgcatgctac tttcggtact caatcatatg aaacttttag
    12181 cggacatctc gctaaagcaa ttgtttctgc aactgctgaa atcattctta ctgtaactga
    12241 tttggacatt caacgttcat tcaaagatgc agtcggttta aatttacttg atcctcaata
    12301 gcactttact ttttagttga atcaaatttt attacaagtt atcataagtg cttaatcata
    12361 aatgcattct cgtttattaa tatacttcaa cgagttaaat taaattttta attaggaatt
    12421 gaatgcgttt ataactatca catttgaatg aataactttt tcattagaaa gtagtgttta
    12481 ttattgatgc aatctcgata catcgctaga atttactttt taattttatt tatcgtctcc
    12541 ctttttgaga ctaaaatttt tcaatttatg actttaaatt tgtttttagc atatatacct
    12601 tttgagctta gtctattatt acgattattc aaaccgaata aaaagtatga atggccactc
    12661 tttatcatat atagttttat atttgtgtta cttttaccaa atacatttta tatggtaact
    12721 gatttaatcc atttgaacca atttcaattc aacttttacg caggactaaa tttaagtgag
    12781 tggaaatact ttacctattt actgcttggt gtcttcttag cggtttatgt gatgatttta
    12841 atctattttg aaatactaac attgacttct caccgttggc ttaatagatt aattgttgtc
    12901 gtgctaatgt ttttaaatgg attcggaatt tatattggtc gtttcttacg cttacattcc
    12961 gtttatttct ttaatgagcc cttacatgtt ctaaatcaca ttctagaaag tctaacgctt
    13021 aaaactgcta tgtttgtatg ttttatggtt gtcatgcaag cagcaattct attatttgga
    13081 aaaggagtgc gtttgaacaa gtgacaataa atataatcgt tttaatagta tctatcattg
    13141 tctttcaatt gattattgga cacatatggc atgatattgg tttaagttat ttaagaagta
    13201 tcttactcat gatgctgcca tttggactag gtgtatttat acaacaagtg tcgtattacg
    13261 aacgacaata tcctaaatgg caagtgcctc aaaacataaa agttagattg aaatatattt
    13321 atctcgctac gtttttagag tatgtcgtac tatacctcac tttatttacg gacatactaa
    13381 gataacatca atttaataag aaaactaaat acaacttata tatttgagga aagccaattt
    13441 ctatcacatc aataatagaa attggcttat ttatattgct taagaacttt atattctgct
    13501 ccagatttta ctcatgccag atctatcaat tgagccgaat tagtctagcg ctttaaatgt
    13561 agctaaaact tgtgtacgtt tttcttgatg atctaccatt tgtcttggat aatgactacc
    13621 taattcaatc ccctgttcaa aaaggttgga ctcattgcgt tgtgtatcat gcaaatattt
    13681 tgcatcaatt tgattaaaaa tcggaagata tgttttgata tacaaagctt tagcatcaaa
    13741 gcgttcactc tgtcttattg gattaaacat tctaaaatac ggcactgcat ccgtacctgt
    13801 agaagcagac cattgccatc catgaatatt tgatgctgca tcatagtcaa taaggtgctt
    13861 tctaaagaat ttttctcccc atgtccaatc tataaataaa tctttggtta aaaattgcga
    13921 cacaaccatt ctcattcgat tatgcataaa accagtttgt gtcaattcca ttattgctgc
    13981 atcaatgatt ggaaatcctg tttgcccttc gcaccatgca ttaaaattcg cttcattttg
    14041 cgaccatttt atctgtcgat atttaggttt gaaagcttgg tatgaggttt ctggatactg
    14101 tgtcattaac acataataaa attctctaaa aatgagttca cgtataaatg cttcatggtt
    14161 tgcctcatct tcatcataac gcgccaataa atcattaaaa atttcaataa tatctaataa
    14221 tccatatgct aaagcaacac ttagctgact tgttaacact tcaggtaaat agtctcttcc
    14281 gctttcgtaa ttctgtatat cttgatctaa aaaatgttgc catttattct gttcaattac
    14341 ttgtgaggat ccaaacgcta tgtcatcaaa cactaaatca tcaggtgatg caatgacaaa
    14401 gtcttcgaat gattttaaat catagtgata tacgtctcta tgcctcaagt aaggtcgcca
    14461 ttttctatag aaactagtaa aaactttgta tggctcccct tgttgattca ttgttttact
    14521 cgctttaaag taatgattcc cacgaattaa agtaacggca atacgtgctt cattgaataa
    14581 agaacgttga tgtaaaatat catattcttc aagatgataa ctcataatat cacccgcaat
    14641 cactacttca gagatattgt tctctttaca aaatgaaatg atatcgtcat aagacataac
    14701 ataaggtgta agcttttctg cgtgcaacga ttggtaaaag cgctgcaaag taccgcgata
    14761 aaactgtgct ttcaaatcag aagctgaaga catgtcctct tccggaatga taaaataaca
    14821 tgcatcaatg tcttcttttt gttgataaat atattcaaat aatggattgt gttccattcg
    14881 aaacattcga tttaataaca cagcaattgc cataaacaaa acacccttca attatctttt
    14941 tattttatta agctttttgt cactttaaaa taacctagat tcttatttat atttttaatt
    15001 aaagcaaacc taaccatttc caataagtca tactaaaaac tatcactaga atatacccta
    15061 caattgtcaa tggtatacct gccttcaaga aatctttaac cgtaaaagta ccagtgccat
    15121 aagccaacat attttgaggt gcactaacag gtaataagaa accaaaacta ataacaaatt
    15181 gttgaattaa aacaaatcct atagactggt ctcctaagtg taacgtagag gttagcgaaa
    15241 taaaaacagg tattaacgct gatgataaac ttgttgcact cgcaaagccc aaatgaatca
    15301 atatattaaa aagcgtgata agtgcaattg tcgcgataat aggtaaatgt tttaaaccta
    15361 aaacaccaaa agtttgatca cttaaccatt gagctgcacc tgttttcaaa agaacgttcc
    15421 ctagtgaaat acctacacca aacacgataa ttgttcccca tggtatttta ttttcaacat
    15481 gtttccatgt catgacacca attttcggca ttaacataac acctaaagca ataatagtaa
    15541 tggatgcaga gtcaatcgga tgtaatactt tttcagttga ccaaaacagt aataataaca
    15601 tcgatataac aattaaacgc cattcacgtg ggctaacggg gccaagttta tgcaattctt
    15661 cttttattaa atctttacca ccttctattg tattaatttc tggaggcatc actttaatca
    15721 tgatgaaata taaagctacg gacataacta tggaccaagg cgctgcatat aagaaccact
    15781 cgccccatga aacatcaaat cctaattgat ggtttataaa attaatcgct acgatatttt
    15841 gtgctgccgc cgttttgata ccaatattcc aaattgacac agcttgtact gaagtaatta
    15901 ttaataaaga cgctaacttg ctatctttgg aaactttaaa tgccgcaatc atacccagca
    15961 agattggtac aactgcccct gctctagctg ttgcagaagg aacgaaaaat gcaagtacaa
    16021 ttgaaacgat aattgctcca ataactatat ttctagtttt attaccaaca attgataaca
    16081 ctaaaagagc tagtctttta tgcaaattcg tttcttgcat agcagcagcc aaaaataatg
    16141 cagctgcaac gagagctaca gctgaagttg catatccact aaacgctaat gataatgcat
    16201 gattagttcc tagaaggtca cttccagcta aaatagcact gccacttttc ggattaccta
    16261 gcttctcccc taaattttga accgggctaa atcctaaaag taatatcatt aagccaataa
    16321 ttaaagttgc tgacaccgga tatgatacag cttccgttac ccacataata actgcaaaag
    16381 ctaaaatagc tagtactgcc ttagccatta tcggcagact tgaaggtgcc gggagtaata
    16441 gtacaactat aagtattata aaactcatga caatccaaaa tggcttgtat tgcttttttg
    16501 tcttttcttt cttttgttgt tcatgttcca ctacatgttt ttcttctgac ataacagtca
    16561 cccctttgtt taatcttaat ttaattataa caatttttta aaacatacat ttttaattta
    16621 tgcaaaatac acaattttat cgaaatattt tgtaaataat attgctaggt gatgagttat
    16681 ctcggttaat tacaaaacga tatctaagtt taaatattag atcataaaaa aactatctac
    16741 tgaatatcct catagcatca tttttctaag caaaagttaa acttagttag ataaaatgca
    16801 aatgagatta ttgtagatag tc
//
LOCUS       DAOBBH010000040        16568 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.040, whole genome shotgun sequence.
ACCESSION   DAOBBH010000040 DAOBBH010000000
VERSION     DAOBBH010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16568
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.040"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(89..256)
                     /locus_tag="P6L01_000979"
     CDS             complement(89..256)
                     /locus_tag="P6L01_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaI family protein"
                     /protein_id="HDP3030063.1"
                     /translation="MRQVQCIICDAKVFIDERTTESKRLKNNPIRTFMCDDCKSRLDT
                     PKQRAQHYPLD"
     gene            complement(258..740)
                     /locus_tag="P6L01_000980"
     CDS             complement(258..740)
                     /locus_tag="P6L01_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030064.1"
                     /translation="MIQIKGAVKFPITLDSTTWIFDDRKVSIEDLEKGVFDGSKPIDF
                     EDNHEWNRAILEGQTNPPTLNSEIKYKKRAMLEGSFVINMTPFFKHSEPHDSATTIRL
                     SNEHDHLDIPIDLLPYLFFQFAKDGKRLYSDNGVDSFIYNQEDGYSYRINHVTHIEVI
                     "
     gene            881..1156
                     /locus_tag="P6L01_000981"
     CDS             881..1156
                     /locus_tag="P6L01_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDP3030065.1"
                     /translation="MAKQATMKNAALKQLTKDADEILHLIKVQLDNLTLPSCPLYEEV
                     LDTQMFGLQKEVDFAVKLGLVDREDGKQIMLRLEKELSKLHEAFTLV"
     gene            1470..2696
                     /gene="ftsW"
                     /locus_tag="P6L01_000982"
     CDS             1470..2696
                     /gene="ftsW"
                     /locus_tag="P6L01_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HDP3030066.1"
                     /translation="MKNFRSILRYIGKTSKFIDYPLLVTYIVLSLIGLVMVYSASMVP
                     ATKGTLTGGIDVPGTYFYNRQLAYVIMSFIIVFFIAFLMNVKLLSNIKVQKGMIITIV
                     SLLLLTLVIGKDINGSKSWINLGFMNLQASELLKIAIILYIPFMISKKMPRVLSKPKL
                     ILSPIVLALGCTFLVFLQKDVGQTLLILIILVAIIFYSGIGVNKVLRFGIPAVLGFLV
                     VFVIALMAGWLPSYLTARFSTLTDPFQFESGTGYHISNSLLAIGNGGVFGKGLGNSAM
                     KLGYLPEPHTDFIFAIICEELGLIGGLLVITLEFFIVYRAFQFANKTSSYFYKLVCVG
                     IATYFGSQTFVNIGGISATIPLTGVPLPFISFGGSSMISLSIAMGLLLIVGKQIKVDQ
                     QRKKQQQKVDIRRQFN"
     gene            3250..6702
                     /locus_tag="P6L01_000983"
     CDS             3250..6702
                     /locus_tag="P6L01_000983"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDP3030067.1"
                     /translation="MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHR
                     YKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEG
                     IKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMI
                     KATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIG
                     DEHGNIVHLFERDCSVQRRHQKVVEVAPSVGLSPTLRQRICDAAIQLMENIKYVNAGT
                     VEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQK
                     DITTLGYAIQCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYD
                     SLLVKLSTHAISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFI
                     EETPELFDIQPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASF
                     SGTKQLLDEVGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTAD
                     VFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRASNAVGYKNY
                     PDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILN
                     PERSNIYTLEYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKSAVDLPIHL
                     HTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDI
                     EGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFD
                     EVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVS
                     FFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTE
                     QDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKL
                     ETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSV
                     TEVKVSVGETVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGDTIATGDLLIEIE
                     KATD"
     gene            complement(6842..7753)
                     /locus_tag="P6L01_000984"
     CDS             complement(6842..7753)
                     /locus_tag="P6L01_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HDP3030068.1"
                     /translation="MFGKKNLKWLGVVATLMMTFVQLGGALVTKTGSADGCGSSWPLC
                     HGALIPEFFPIDTIIELSHRAVSALSLLMVLWLVITAWKHIGYIKEIKPLSIISVGFL
                     LLQALIGAAAVIWQQNDYVLALHFGISLISFSSVFLITLIIFSIDQKYEADELYIKKP
                     LRRLTWLMAIIIYCGVYTGALVRHADASLAYGGWPLPFHDLVPHSEQDWVQLTHRIMA
                     FIVFTIIMITYIHAVKNYPNNRTVHYGYTAAFILVILQVITGALSIMTNVNLIIALFH
                     ALFITYLFGMTTYFIMLMLRSVRSDKQ"
     gene            8193..9104
                     /locus_tag="P6L01_000985"
     CDS             8193..9104
                     /locus_tag="P6L01_000985"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDP3030069.1"
                     /translation="MSKEHTLSQNISRVNFKELQQIIKMGLVQGNLIPAFAGAWLAVV
                     MTNHSFLSSIPQILLMLFGSTLIMGGACALNNYYDQDIDRIMPSKQNRPTVNNRITDQ
                     NLLLLSFGMMLVGEICLFLLNIPSGVLGLMGIVGYVSYYSIWSKRHTTWNTVIGSFPG
                     AVPPLIGWVAIEGQISLTAIALFLVVFCWQPIHFYALAIKRKDEYALANIPMLPSVKG
                     FKRTRVSMFIWLIILLPVPLLLINLGVVFVVLATLLNLGWIALGLTTFKKNSDQTKWA
                     TQMFIYSLNYLVIFFVLAVIVSLLTLI"
     gene            9129..9590
                     /locus_tag="P6L01_000986"
     CDS             9129..9590
                     /locus_tag="P6L01_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HDP3030070.1"
                     /translation="MGVPILPTISTTCIVISAILIAIGWRLIWKREINKHKNVMLAAA
                     VFALTFFLIYASRTIFIGNTAFGGPASIKKYYTIFLFFHINLATIGGILGLVQIITAF
                     KDKYNVHRKFGPFASVIWFCTAITGVAVYLLLYVLYPGGETTSLIKATFGH"
     gene            9917..10954
                     /locus_tag="P6L01_000987"
     CDS             9917..10954
                     /locus_tag="P6L01_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HDP3030071.1"
                     /translation="MKKLIIRVVGVLFLVGFLIYLFYSPRLKFDVLENPNKGNKVNRS
                     EQVNKSNNHAENPKPKEGVGTWVGKDIKVLTSKFGQADRVYPFRDGYKNYVFKDKNSY
                     YIVSTKREEIVSVYATGEKVNVSPLKIGQHSAEIFNHTSINPEPSFKVDGKKYEFELS
                     DEDLKTQTLIKYGDIYAQVYSDQQSKKVLSVRFLTKEMLADIEPYQLNSNSASEEHNK
                     RPVEQNPNQLISLYEVTNEMRKLKGLKPLKINSDLAHIASNNLYEATSNGSDSVEFTE
                     DALRGQLDKNHVTYKTTAQNVGYAFNDVPTLIHSWMNSDIHRSRLLNSKYDEMGGDVM
                     RDYYSLIFLEK"
     gene            10970..11404
                     /locus_tag="P6L01_000988"
     CDS             10970..11404
                     /locus_tag="P6L01_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDP3030072.1"
                     /translation="MINEASLAILDDIDELADMIVASDIYASFEQAKQALENNDEAHL
                     LYQSFLKSKEKYDEVMRFGKYHPDYKKVMLETRQRKRAYEMLDVVMHYKAREMALQHL
                     IDEVVTKIAYAVSEHVKIETGNPFFQTSHSGCATGGSCNCSL"
     gene            complement(11467..12393)
                     /locus_tag="P6L01_000989"
     CDS             complement(11467..12393)
                     /locus_tag="P6L01_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDP3030073.1"
                     /translation="MKKVNRNILNAVLVGAGFLSSLLMINKNKVITKKQTIPAFFKGN
                     APYIFAHRGGMALRPEQTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTN
                     GSGKVSAHTLAELKKLDAAYHFKDINGLTPYRGHAHTTILTFDELLKQYPDMYINVDL
                     KDAPESYEGSIAPQIMFDTIAENQAFDRVLVTSFYKEQIVRFNKIAQGSVAIGASQQE
                     VTEAFLKYHLLGGRYYQPLAQTFQMPTHFKGIDLTSSRFIKWLNDMNIIPGYYGVNSI
                     DLMNDLYQKGAHTIVTDRPDLAQQFKQTIPNK"
     gene            12632..12886
                     /locus_tag="P6L01_000990"
     CDS             12632..12886
                     /locus_tag="P6L01_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDP3030074.1"
                     /translation="MNLIPRTSIVVYLKHMKHERQIRKYGHIVHSNRDRKFVIMYVNE
                     QDVDQIVHKLMQLKYVRHIDGSPYKYLKKTYEKEKHEIYN"
     gene            complement(12889..13278)
                     /locus_tag="P6L01_000991"
     CDS             complement(12889..13278)
                     /locus_tag="P6L01_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030075.1"
                     /translation="MKQTFITLGEGLTDLFEFMTMIEYNHQRIDKIIYFHSPQAENKK
                     SSVAIIMNPTTGNHFQAFYIMINAIKYPYPDSNKKFQMINDCAEKFDIPILGIDVQPP
                     QAFHDLSLYYNYLISVLRLQKWIPELQ"
     gene            13348..13890
                     /gene="rsmD"
                     /locus_tag="P6L01_000992"
     CDS             13348..13890
                     /gene="rsmD"
                     /locus_tag="P6L01_000992"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDP3030076.1"
                     /translation="MRVIAGKHKSKALESMEGRNTRPTMDKVKEGIFNSLYDVSGIGL
                     DLFAGSGALGIEALSRGMDKVIFVDQNFKAVKVIKSNLANLDLEAQSEVYKNNADRAL
                     KALSKRDIQFDVIFLDPPYNKGLIDKALKLISEFNLLKENGIIVCEFSNHEEIDYQPF
                     NMIKRYHYGLTDTLLLEKGE"
     gene            13892..14374
                     /gene="coaD"
                     /locus_tag="P6L01_000993"
     CDS             13892..14374
                     /gene="coaD"
                     /locus_tag="P6L01_000993"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDP3030077.1"
                     /translation="MEHTIAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKE
                     GTFSLEERMDLIEQSVKHLPNVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELR
                     LTSMNKKLNNEIETLYMMSSTNYSFISSSIVKEVAAYRADISEFVPPYVEKALKKKFK
                     "
     gene            complement(14436..15575)
                     /locus_tag="P6L01_000994"
     CDS             complement(14436..15575)
                     /locus_tag="P6L01_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDP3030078.1"
                     /translation="MKSVGLITEYNPFHNGHQYHINQSKKLTNADVTIAIMSGNFVMR
                     GEPAIYNKFTRAKMALSTADLVIELPATASLSSGDHFAELAVKVADYMSVDTIAFGSE
                     NNDIKTLKQLAHSINEIEQSESFSQKVKEGKSYPRIISELLEHHEALASPNNILGISY
                     LKAIAKNAKNINAISIKRENAQHHDSLIQHHQFASGTSIRTSIISQDDHWHHVVPKDI
                     QHLYVTPHITLNQIFPYLKYQIIAMTTDSLKNIYTVTEGFENRLKSNIYEATDFHHFV
                     KLLKTKRYTYTHIQRLLMNVLLNIKPTDVTSNIHAVKVLAMNDRGRQYLKHLKTAFPE
                     RQYITNINKSNAHYFTNEIKATHIYNAISGQQQTDFNTPVIQQYR"
     gene            15702..16259
                     /locus_tag="P6L01_000995"
     CDS             15702..16259
                     /locus_tag="P6L01_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDP3030079.1"
                     /translation="MKWSITQLRKYQGKPFEFDQTVSFDNLKESLDLIDLSPITIQGQ
                     LTIKSTEVVADIHITGTYTMPCARTLVPVKVPLDVTTTEVFDLEGYNQYNDDQDDVDE
                     HYHIIKDGMVNLQDIVEDIVIIEKPMRAYSEQSDQMLTVGNGWEVIDEDQLDELAKQQ
                     EQDDSESRQVDPRLQKLQQLYDKEQ"
     gene            16339..16512
                     /gene="rpmF"
                     /locus_tag="P6L01_000996"
     CDS             16339..16512
                     /gene="rpmF"
                     /locus_tag="P6L01_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDP3030080.1"
                     /translation="MAVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCK
                     NCGSYNGEEVAAK"
BASE COUNT         5704 a         2345 c         2977 g         5542 t
ORIGIN      
        1 ttggcatata atatttgaaa aagtgtttaa atgctattat ttcctgttac tttcacattt
       61 tcaaatttct aacttgttta ttcagatatt aatcgagcgg atagtgttgc gcacgttgtt
      121 taggtgtgtc taatcgactt ttacaatcat cacacataaa tgttcgaata ggattgtttt
      181 taagtcgttt agactcagtt gtgcgctcat ctataaatac ttttgcatca cagataatgc
      241 attgcacttg tctcaaatta aatcacctca atatgagtta catggttgat tcgatatgaa
      301 taaccatctt cttgattgta aataaaacta tctacaccat tatcgctgta aagtcgttta
      361 ccgtcttttg caaattggaa aaataaatag ggtaatagat ctatcggtat atctaaatga
      421 tcatgctcat ttgataatcg aatagtagtt gcagagtcat gtggttcgga atgtttgaaa
      481 aatggtgtca tattaatgac aaatgaacct tctaacatgg cacgtttttt atattttatt
      541 tctgaattta aagtaggcgg attagtttgt ccttctagga tagcacgatt ccattcatga
      601 ttatcttcaa agtcgattgg ttttgaacca tcaaatacac ctttttctaa atcttcgatg
      661 ctaacttttc tatcatcgaa aatccaagtc gtactatcta atgttatagg aaactttacg
      721 gctcctttaa tttgtatcat tttcccactc cctatcaatg tatatagcat tattttaaca
      781 caaattgcta acgacacatt ttaaatctac ttgcttttta atatttagta agataaactt
      841 ttagtaagac ttgagaattt atatagaggg ggagcgtgtc atggcgaaac aagcaacaat
      901 gaaaaatgca gctttgaaac aattgactaa agatgctgat gaaatcttgc atctgattaa
      961 agttcaacta gataatttaa cattaccttc atgcccatta tatgaagaag tactagatac
     1021 acaaatgttt ggacttcaaa aagaagttga ttttgctgtt aaattaggtt tagttgaccg
     1081 cgaagatggc aaacaaatta tgttacgtct tgagaaagaa ctttcaaaat tacatgaagc
     1141 ttttacactt gtttaaggtt taatatataa gcatattaaa atggcgtgag atatcgacaa
     1201 ttgagctgcg ccgattttat atttatagtt taattactga ttaaagtggc tgttatgttg
     1261 tcttcttgat gacatgtcag tcacttttat tgggttttag caattcacga tattaagtaa
     1321 acaaatattc tataaattac ctcaagagac ctgttaatat ttgtgattat gactgtgtca
     1381 tgtatataat aagtaacatg gatttttaaa attaaagtat aaaacgaaat catttgaagt
     1441 ataaattgta gaattggatg actgaatata tgaagaattt tagaagtatt ttacggtata
     1501 ttggtaaaac ctcaaagttt attgattatc cgttattagt tacatatatt gtattgagtt
     1561 taattggatt agtgatggta tatagtgcaa gtatggttcc agcaactaaa ggcacattga
     1621 ctggtggtat cgatgttcca ggaacgtatt tttacaaccg acaattagca tatgtcataa
     1681 tgagttttat aattgtattt tttattgcat ttttaatgaa tgttaaatta ctgagtaata
     1741 ttaaagtgca aaaaggtatg attataacta tcgtctcact attattactg acgttagtaa
     1801 taggtaaaga tattaatggt tctaaaagtt ggataaactt aggatttatg aacttacagg
     1861 catctgagtt attaaaaatt gcaatcatat tatatatccc atttatgatc agtaaaaaaa
     1921 tgcctagagt attaagtaaa ccaaaattaa ttttaagtcc tattgtatta gcattaggtt
     1981 gtacgttttt agttttctta caaaaagacg tagggcaaac attactaata ttaattattt
     2041 tagttgcgat tattttttat tcaggaattg gggtaaacaa agtcctaaga tttggtatac
     2101 cagcagtgct aggattctta gtagtatttg tcattgcatt aatggctggt tggttaccaa
     2161 gctatttaac tgccagattt agtacgctaa cagatccatt ccaattcgaa tcaggaactg
     2221 gataccatat ttccaattca ttgcttgcga taggtaacgg tggcgtattt ggaaaaggat
     2281 taggaaatag tgcaatgaaa ttgggctatt taccagaacc acatacagat tttatttttg
     2341 caattatttg cgaagaatta ggtttaatcg gaggattgct agttattact ttagagttct
     2401 ttattgtata tcgtgccttc cagtttgcaa ataaaacatc atcatatttt tataaacttg
     2461 tgtgtgttgg gattgccaca tactttggaa gtcaaacgtt tgtaaacatt ggcggtattt
     2521 cggcaacaat tccattaact ggtgtgccat tgccatttat cagctttggt ggatcatcaa
     2581 tgattagttt aagtattgct atgggattac ttctgattgt aggtaaacaa atcaaagtag
     2641 accagcaacg aaagaaacaa caacaaaaag ttgatataag aagacaattt aattaaaaaa
     2701 atactagcca atatttagta cgagatgact tctgtcatta agcaatgcta aacattggct
     2761 atttatatac tctttgtcaa attggactga tgagttcaat aacggaagtt aggcaaccaa
     2821 acaacgctta acttcctttt tgttttgata ttacgagatt actcatgatt atcaaatttt
     2881 ataaacaaaa caacgtataa ttataactaa attttatgac taaaaattac taaatgtggt
     2941 ggcgaaatta gaatcatgat gataagataa agttatcgct attgataata aatgttgatg
     3001 ttttgcaatt ttatttttaa ttttttagag ttgtagaaat cgaaaatgtt tcacaacaaa
     3061 aacgtgaact acctgttgtt gcatagtcat ttttgaaatt attatttctg aagcggaggt
     3121 tttaattgtc tcaagaatgt taaattttga aaatttgaaa cataactata gcaaaacaga
     3181 gcgctttaag ataaaatttt attatctaat tagttagaaa attctaatag tttgaggagt
     3241 gtatcgctat tgaaacaaat aaaaaagtta cttgttgcta accgtggaga aattgcaatt
     3301 cgtatattca gagcggcggc agaattagac atcagcacag ttgcaattta ttcgaatgaa
     3361 gacaaaagtt cattacatag atataaagca gatgaatcct atttagttgg aagtgattta
     3421 ggtcctgctg aaagttattt aaatattgag cgtatcattg atgtagcaaa acaagcgaat
     3481 gtggatgcga ttcatcctgg ctatggattt ttaagtgaaa atgaacaatt tgcgcgtcgt
     3541 tgtgcagaag aaggaattaa atttattggt cctcatttag aacatttaga tatgtttgga
     3601 gataaagtta aagctcgtac aacggctatc aaggcagatt taccagttat tcctggtaca
     3661 gacggtccaa ttaaatcata tgaattagca aaagaatttg cagaagaagc tggtttcccg
     3721 ctaatgatta aagccacaag tggtggcggc ggtaaaggta tgagaatcgt tcgtgaagaa
     3781 agtgaattag aagatgcttt ccatagagca aaatcagaag ctgaaaaatc atttggtaat
     3841 agtgaagttt acatagaaag atacattgat aatccaaagc atattgaagt acaagtcata
     3901 ggtgacgaac atggaaatat cgtacactta tttgaacgtg attgttcagt acaacgtcgt
     3961 catcaaaaag ttgtagaagt tgcaccatca gttggattat caccaacatt acgtcaacgt
     4021 atttgtgatg ctgcaattca attgatggaa aatattaaat atgtcaatgc aggtactgtt
     4081 gaatttctag tatctggtga cgaattcttc tttatagaag ttaaccctcg tgtacaagta
     4141 gagcatacaa ttacagagat ggtaacagga attgatattg ttaagacaca aattttagtt
     4201 gcagcaggtg ccgatttatt tggtgaagag attaatatgc cgcaacaaaa agatattaca
     4261 acattaggct atgccatcca atgtcgtatt acaacagaag atccgttaaa tgatttcatg
     4321 ccggatactg gaacaatcat tgcttatcgt tcaagcggtg gctttggtgt acgtcttgat
     4381 gctggagatg gtttccaagg tgctgagata tcaccttatt atgattcatt actcgtaaaa
     4441 ttatctacac atgcgatatc atttaaacaa gcagaagaaa aaatggtacg ttcattacga
     4501 gaaatgcgta ttcgtggtgt taaaactaat attccattct taattaatgt aatgaagaat
     4561 aaaaagttca caagtggtga ttacacaact aaatttattg aagaaacacc agaacttttc
     4621 gacattcagc cgtctctaga tagaggtact aaaacattag aatatatagg taatgtaaca
     4681 attaatggtt tcccaaatgt tgagaaacgt ccgaaaccag actatgaatt agcatcaatt
     4741 ccaactgtat cttcaagtaa aatcgcttca tttagtggta cgaaacaatt gcttgatgaa
     4801 gtaggtccaa aaggtgtagc tgaatgggtt aaaaagcagg atgatgtctt actaacagat
     4861 acaaccttta gagatgcaca ccaatcatta ttagctacac gagttagaac taaggatatg
     4921 attaatatcg catccaaaac agcggacgta tttaaagatg gtttctcact agaaatgtgg
     4981 ggcggtgcta catttgatgt ggcatataat ttcttgaagg aaaacccatg ggaacgactt
     5041 gaacgtctac gtaaagctat cccaaatgta ttattccaaa tgttgttacg tgcttcaaac
     5101 gcagttggtt ataaaaacta tcctgataat gttattcata aattcgtaca agaaagtgct
     5161 aaagcaggca tagatgtctt tagaattttc gattcattaa actgggtaga tcaaatgaaa
     5221 gttgccaatg aagcagtaca agaagcgggc aaaatctctg aaggtactat ttgttataca
     5281 ggtgacattt taaatcctga gcgatcaaac atttatactt tagagtatta tgtcaaacta
     5341 gctaaagagt tagaacgtga aggtttccat attttagcga ttaaagatat ggcaggctta
     5401 ttaaaaccta aagccgctta cgaattgatt ggtgagttaa aatcagctgt agatttacca
     5461 attcatcttc acactcatga tacaagtggt aatggtttat taacatacaa acaagcaata
     5521 gatgctgggg tcgatatcat tgatactgct gttgcttcaa tgagtggttt aacaagtcag
     5581 ccaagcgcca attcgttata ttatgcatta aatggcttcc cacgccacct tagaactgat
     5641 attgaaggta tggagtcact tagtcattat tggtcaactg tacgtactta ttattcagac
     5701 tttgaaagtg atatcaaatc accgaatact gaaatttatc aacatgaaat gcctggtgga
     5761 cagtattcga atttaagtca acaagctaaa agtttaggtt taggcgaaag atttgatgaa
     5821 gtcaaagata tgtatcgcag agtgaatttc ttatttggtg atatcgtaaa agtaactcca
     5881 tcgtctaaag tagttggtga tatggcactt tatatggtac aaaatgatct tgatgaacaa
     5941 tccgtgatta cagatggcta taaattagat ttcccagaat cagtagtgtc gttcttcaaa
     6001 ggcgaaatag gacaacctgt aaatggtttt aataaagatt tacaagcggt tattttaaaa
     6061 ggccaagaag cactaacagc tcgtccaggt gaatatctag agccagttga ttttgaaaaa
     6121 gtccgtgagt tgcttgaaga agagcaacaa ggtcctgtta cggagcaaga tattattagt
     6181 tatgtattat atccaaaagt atatgaacaa tatattcaaa ctagaaatca atacggaaac
     6241 ttatcgttac ttgatacgcc tacattcttc tttggaatgc gtaatggtga aacagtagaa
     6301 atcgaaatcg ataagggtaa acgattaatt attaaactag aaacgattag tgaaccagat
     6361 gaaaatggta atagaacgat ttactatgcg atgaatggtc aagcgagacg tatttacatt
     6421 aaagatgaaa atgtgcatac aaatgcgaac gttaagccaa aagcagataa gagtaatcca
     6481 agtcatatcg gtgctcaaat gccaggttca gtaactgaag tcaaggttag tgttggtgaa
     6541 actgtgaaag ctaatcagcc gttgctaatt actgaagcta tgaaaatgga aacaacaatt
     6601 caagcaccat ttgacggtgt gattaaacaa gtaactgtaa ataatggtga cacaatagcg
     6661 acaggcgatt tattaatcga aattgaaaaa gcaactgact aaaatgatta aaataaaacg
     6721 agattacaca acaattgacg tgtaatctcg tttttacatg gcttaggttt aattttaaat
     6781 attgatataa aaaaccgtaa ttttaatggg cgtgccatta aaattacggt ttgctttttt
     6841 gttattgctt gtcacttctt actgatcgta acattagcat gataaagtat gttgtcatac
     6901 caaataaata agtgataaat aatgcatgga aaagtgcgat tatcaggtta acatttgtca
     6961 taatagataa tgcacctgtg ataacttgta atataacaag tataaatgca gctgtataac
     7021 cataatgaac agtacgatta tttggataat ttttaacagc gtgaatataa gtaatcataa
     7081 taatcgtaaa cactataaaa gccatgatac gatgcgtgag ttgaacccaa tcttgttctg
     7141 aatgtggtac aagatcgtgg aatggcaatg gccaaccacc atatgctaaa cttgcatccg
     7201 catgtctcac tagtgcacca gtataaacac cacaataaat gatgattgcc attaaccatg
     7261 ttaaacgtct taatggcttt ttgatatata attcgtcagc ttcatatttt tgatctatag
     7321 agaaaataat caatgttatt aaaaatacag atgagaaact gattaatgat ataccaaagt
     7381 gcaatgctaa aacgtaatcg ttttgttgcc aaataacagc ggcagctccg attaatgctt
     7441 gcaataataa gaatccaaca ctaatgattg ataaaggttt aatttcttta atatagccta
     7501 tatgtttcca tgcagtgata actaaccata agaccattaa taaagacaaa gctgaaacgg
     7561 ctctatgact taactcaata atcgtatcaa taggaaagaa ttctggaatc aacgcaccat
     7621 gacatagtgg ccaagaagaa ccacaaccat cagctgatcc ggttttggta actaaggctc
     7681 caccaagttg tacaaatgtc atcattaacg ttgctacgac acctaaccat tttaaattct
     7741 ttttgccaaa caatttatac accccatcat aaaaaaacgc atcactgaga attagaatta
     7801 atagccggat ttgagatgat actataaatt gacctgattg taggatatga aaagatggga
     7861 tccaacgcag gaaatatgat ttaattttac aagtattgta tgtggacgac ataaaaagaa
     7921 cttaatcgta atgatttttt tattgggata attatgtaat ccacgtttac tcaaatttga
     7981 agcttaaata ttaatctctt ttagatgcat gattctactt attaaaattt tctaacatat
     8041 acattatagc aataaattaa atgttatttg tgtcacaaaa ttgacatttt ctcttgtcgt
     8101 taatttgtca cattttaatt ttattaaacc tagtcttttt atgcaatcta agatatcata
     8161 atcttatatg aacaaattta aggaggggga ttatgagcaa agagcatact ttgtcacaaa
     8221 atattagcag agttaacttc aaagaattgc aacagataat taaaatggga cttgttcaag
     8281 gtaacttaat tccggctttt gcgggcgcat ggctagcagt tgtaatgaca aatcattcct
     8341 tcttatcatc aatacctcaa attttattaa tgctatttgg atctacttta attatgggtg
     8401 gcgcatgtgc gttaaataat tattacgacc aagatattga tcgtattatg cctagtaaac
     8461 aaaatagacc aactgtaaat aatagaatta cagatcaaaa tttattacta ttaagttttg
     8521 gtatgatgtt agttggagaa atttgtttgt ttttattgaa tataccatca ggcgtacttg
     8581 gtcttatggg gattgtaggt tatgtgtctt attactcaat atggtctaaa agacatacaa
     8641 catggaacac agtgattggg agttttcctg gagcagtacc accactaatt ggatgggttg
     8701 caattgaagg acaaattagt ttaacagcga ttgcgctgtt tttagttgta ttttgttggc
     8761 aaccaattca tttttatgcc ttagctatta aacgtaaaga tgaatatgca cttgcaaata
     8821 ttccaatgtt accatcagtt aaaggcttta aacgtacacg tgtcagtatg tttatctggt
     8881 tgattatttt attgccagta cctttattac taataaattt aggtgtcgta ttcgtagtgt
     8941 tagctacatt attaaattta ggatggattg cattaggttt aacaacattt aagaaaaatt
     9001 cagatcaaac aaaatgggca acacaaatgt ttatatattc actaaattat ttagtgatct
     9061 ttttcgtgtt agctgtaatt gtttcattac ttactttgat ctagattaat taagttagga
     9121 tgaaaaatat gggcgttcca attttaccaa cgattagtac gacatgtatt gtcattagtg
     9181 caattttaat tgccattggt tggagactta tttggaaaag ggaaataaat aaacacaaaa
     9241 atgttatgtt agctgcagct gtttttgctt taactttttt cttaatctat gcaagtagaa
     9301 cgatttttat cggtaataca gcatttggcg gaccagcatc aattaagaaa tattatacga
     9361 ttttcttatt tttccacatt aatttagcaa caattggtgg tattctaggc ctagttcaaa
     9421 ttattactgc atttaaagat aaatataatg tacaccgcaa attcgggcca ttcgcttcag
     9481 ttatatggtt ctgtactgca attacaggtg tagcagttta cttattatta tatgtattat
     9541 atccaggtgg agaaacgaca tcactgatta aagcaacatt tggtcattaa tatgcttata
     9601 caaatgatac taatctgata aggcaatttg ttttatcagg ttttttgatg aagaatagtc
     9661 ggacagttga tggtattcat ttgatacctt aactgtccat ttttttatac atattttaat
     9721 cttaataatg aaattcaacc tgaaaacata aagataggtt atgatataga gtgtagggaa
     9781 aatctttatg acgttaaaat tatgcctgaa gatgattttg ttttatcgaa acgacacagt
     9841 tatgtcagtg ttgaaaattt gctaattaat gtgttgtttt ttacaatagt attaatactt
     9901 tttaggtggt tgattgatga aaaagttaat tataagagtc gtcggtgtat tatttttagt
     9961 tggattttta atatatcttt tttattcacc gagattaaaa tttgatgtat tagagaaccc
    10021 gaataaaggt aataaagtaa atagatctga acaggtgaat aaatcaaata accatgctga
    10081 aaatccaaag cctaaagaag gtgttggtac atgggtaggt aaagatatta aagtgcttac
    10141 ttctaaattt ggacaagcag atcgtgtcta cccttttaga gatggttaca aaaattatgt
    10201 gtttaaagac aaaaacagtt attacattgt ttcaactaaa cgagaagaaa tcgtttcagt
    10261 gtatgctaca ggtgagaaag tcaatgttag tccgttaaaa ataggacaac attctgcaga
    10321 aatttttaat catacaagta ttaatccaga accttccttt aaagttgatg gtaaaaaata
    10381 tgaatttgaa ctttcagatg aagatttaaa aacacaaaca ctgattaaat atggcgacat
    10441 atatgcacaa gtgtattctg atcaacaatc gaaaaaggtg ctaagtgtac gatttttaac
    10501 aaaagaaatg ttagcagata ttgaacctta tcaattaaat tctaattctg cgtcagaaga
    10561 gcataataag cgtccagttg agcaaaatcc aaatcaatta atttctcttt atgaagtaac
    10621 gaatgaaatg agaaaattaa aaggattaaa accattgaaa atcaatagcg atttagcaca
    10681 tattgcatct aataacttat atgaagcgac ctctaatggt tctgatagtg ttgaatttac
    10741 agaggacgca ttaagagggc aattagataa aaatcacgtt acttataaaa caactgctca
    10801 aaatgttggt tatgcgttta atgatgtacc aacattaatc catagttgga tgaattcaga
    10861 tatacatcga tctcgtctat taaattcaaa atacgatgag atgggtggag atgtaatgag
    10921 agattattat tcactaattt tcttagaaaa ataacaatga ggggtattta tgattaatga
    10981 agcttcacta gcgatattag atgatattga tgaactagct gatatgatag ttgcatcaga
    11041 tatttatgca tcatttgagc aagctaaaca ggcgctcgaa aacaatgacg aagcacattt
    11101 actttatcaa tcatttttaa aatcaaaaga gaaatatgat gaagtaatgc gtttcgggaa
    11161 atatcatcct gattataaga aagtcatgtt agagacaaga caacgcaaaa gagcatatga
    11221 gatgcttgac gttgtgatgc attacaaagc tagggaaatg gcacttcaac atttaattga
    11281 tgaagttgtt acgaaaattg cgtacgctgt ttcagagcat gtcaaaatag aaacaggtaa
    11341 tccattcttt caaacatcac atagtggttg tgcgacgggc ggatcctgta attgttcatt
    11401 ataaaaaaca tcgagtcaga aaaaggtggt tattgaaacc actaactagc atctgactcg
    11461 atgtttttat ttattcggga ttgtttgttt gaattgttgt gctaaatctg gtcgatctgt
    11521 cacaatcgtg tgtgcacctt tttggtataa atcattcatc agatctatac tatttacgcc
    11581 ataatagcct ggaatgatat tcatatcatt taaccatttg ataaaacgag atgaagtcaa
    11641 atcaatgcct ttaaaatgag taggcatttg gaacgtttgt gctaatggtt ggtagtacct
    11701 accacctaat aaatgatatt ttaaaaatgc ttctgtaact tcctgttggc tagcaccaat
    11761 tgcgacggat ccttgtgcaa ttttattaaa acgaacgatt tgttctttat aaaaacttgt
    11821 cacaagaacg cggtcaaatg cttgattttc tgcaattgta tcaaacataa tttgtggtgc
    11881 gattgagcct tcataggatt caggagcatc ttttaagtct acgtttatat acatatcagg
    11941 atattgcttc agcaactcat cgaaggttag tatagttgtg tgtgcatgac cacgatatgg
    12001 tgttagtcca ttgatatctt tgaagtgata agctgcgtct aatttcttta attctgctaa
    12061 tgtgtgggca ctaacttttc cagagccgtt cgtcgttcta tcaacagttg cgtcatgaaa
    12121 aacgataagc tgttgatctt ttgtgagtct cacatctgtt tcaaagccat caacgcctaa
    12181 ttgtttagca tagtcaaatg caagttgcgt ttgctctggt cttaaagcca taccaccgcg
    12241 atgcgcaaat atatatggtg cattgccttt gaaaaaagca gggatggttt gctttttagt
    12301 aatcacttta tttttattga tcattaatag actacttaaa aatccagcac cgactagtac
    12361 cgcatttaaa atgtttctgt ttactttttt catagaaaat tcctcctcgt ttacttttat
    12421 agcttaacaa aatcatagtt ctgactacag cgaaatgtgt ggttaatcat atagtgatgc
    12481 tcttgagaat tgaattaata taatgatgtt taatataatc taaatacatt tatcgagcaa
    12541 ttacataaga cataattaag gtagcttaat attttaaaat taataaagac atgatatgat
    12601 taatgctgaa aataaatgga gtgaggttta aatgaattta atcccaagaa ctagtattgt
    12661 agtttattta aaacatatga aacatgaacg acaaatccga aaatatggac atatcgttca
    12721 ttcaaataga gatcgtaaat ttgtaattat gtatgtgaat gagcaggatg ttgatcaaat
    12781 tgtacataaa ctaatgcaac ttaaatacgt tagacacatt gatggctcac catataaata
    12841 cttaaagaaa acttacgaaa aagagaaaca cgaaatatat aattaatatt attgaagttc
    12901 tggtatccat ttttggagcc ttaacacact aattaaataa ttataatata acgataaatc
    12961 atgaaatgct tgagggggct gtacatcgat acctaaaatt ggtatgtcga atttttcagc
    13021 acaatcatta atcatttgaa actttttatt tgaatctgga tatggatatt taatagcgtt
    13081 aatcatgata taaaatgctt ggaaatgatt gccagttgta gggttcataa tgattgctac
    13141 agatgacttt ttattttcag cttgtggtga atgaaaatag ataattttat caatacgttg
    13201 atggttatat tcaatcatcg tcatgaattc gaacaaatct gttagacctt caccaagtgt
    13261 aataaatgtt tgtttcattg gtttatcatt ctcctttttc tcaataaatt atattaaata
    13321 actaaatgaa ttggaagtgt gaacaacatg cgcgtcattg caggtaaaca taaaagtaaa
    13381 gctttagaaa gtatggaagg ccgtaatacg agaccaacta tggataaagt taaagaaggt
    13441 atctttaata gtttatatga tgtgtcaggt ataggtttag atttatttgc aggaagcggg
    13501 gcgcttggaa tagaagcact ctctcgaggt atggataagg taatctttgt tgatcaaaat
    13561 tttaaagctg taaaagttat taaatcaaat cttgcgaatt tggatttaga ggcacaatct
    13621 gaagtttata aaaataatgc agatagagct ttaaaagcat tgtcaaaacg tgatattcaa
    13681 tttgatgtca ttttcttaga tccaccttat aataaaggtc tcattgataa agctttaaaa
    13741 ctaatttcag agtttaattt attgaaagaa aatggtatca tcgtttgtga atttagcaat
    13801 catgaagaaa tagactatca accgtttaat atgattaaac gttaccatta tgggttgaca
    13861 gacacattgt tattagaaaa gggagaatag catggaacat acaatagcgg tcattccggg
    13921 tagttttgac cccattactt atggtcattt agacattatt gagagaagta cagatagatt
    13981 tgatgaaatt catgtctgtg ttcttaaaaa tagtaaaaaa gaaggtacgt ttagtttaga
    14041 agagcgtatg gatttaattg aacaatctgt taaacattta cctaatgtca aggttcatca
    14101 atttagtggt ttactagtcg attattgtga acaagtagga gctaaaacaa tcatacgtgg
    14161 tttaagagca gtcagtgatt ttgaatatga attacgctta acttcaatga ataaaaagtt
    14221 gaacaatgaa attgaaacgt tatatatgat gtctagtact aattattcat ttataagttc
    14281 aagtattgtt aaagaagttg cagcttatcg agcagatatt tctgaattcg ttccacctta
    14341 tgttgaaaag gcattgaaga agaaatttaa gtaataaaaa taacagtatt ttaggtttat
    14401 catggtttac aatcctaaaa tactgttttc atttgttaac gatattgctg tatgacaggc
    14461 gtgttgaaat ctgtttgttg ttgcccgctt attgcattgt atatgtgtgt tgctttgatt
    14521 tcatttgtga agtaatgtgc attgcttttg ttaatattgg ttatatattg tctttctggg
    14581 aacgctgttt ttaaatgctt taaatattgt ctgccacggt cgttcatcgc taatacttta
    14641 actgcgtgaa tgttactcgt aacatctgta ggtttaatgt ttaataatac attcattaac
    14701 agtctttgga tatgcgtata tgtataacgc tttgttttta gtaattttac aaaatgatga
    14761 aaatcagttg cttcataaat gttagatttc aaacgatttt caaaaccttc agtaacagta
    14821 taaatatttt ttaatgaatc tgtagtcata gctatgattt gatatttcaa atatggaaat
    14881 atttgattta atgttatatg aggtgttacg tacaagtgtt gaatatcttt aggtaccaca
    14941 tgatgccaat gatcatcttg actaatgatt gatgttctaa tagatgtacc acttgcaaac
    15001 tgatggtgtt gaattaatga atcatgatgt tgagcatttt ctcgtttgat agaaattgca
    15061 ttgatgtttt tagcattttt agcaattgct ttcaggtaac taataccaag tatgttgtta
    15121 ggacttgcta gtgcttcatg atgctctaat aattcgctaa tgatacgagg gtagctttta
    15181 ccttctttta ctttttgtga aaaggattca gattgttcaa tttcattaat gctgtgtgct
    15241 aattgcttta atgttttgat atcattattt tcactaccaa atgcaattgt atcgacactc
    15301 atataatctg cgactttaac tgctagttcg gcaaaatgat cgccagatga taaactggca
    15361 gttgctggta gttcgataac taaatcagct gttgataatg ccatttttgc acgagtaaac
    15421 ttattataga ttgctggttc gccacgcatg acaaagttac cactcattat tgcaatagta
    15481 acgtcagcat ttgtaagttt tttagattga ttaatatgat attgatgccc attatgaaag
    15541 ggattatatt ctgtgattaa gccaacgctt ttcatttgca attcgtcctt tcggtattca
    15601 ttattaatat tgtaacagaa catgatatgt taagaaaaaa tcttgacaac ttgttcttag
    15661 aaagttaaaa taaattttgt gcttgttaga ggtgaagcca tatgaaatgg tcaattacgc
    15721 aattaaggaa atatcaaggt aagccatttg aatttgatca aacggtgagt tttgacaatt
    15781 taaaagaatc attagattta attgatttat ctccaattac aatccaaggt cagttaacca
    15841 ttaagtcaac agaagtcgtt gcggatattc acattactgg aacgtataca atgccttgtg
    15901 cacgtactct tgtaccagta aaagtcccac tagatgtaac tactacagaa gtatttgatt
    15961 tagaagggta caatcagtat aacgatgatc aagatgatgt agatgaacac tatcacatta
    16021 ttaaagatgg tatggttaat cttcaggata ttgtcgagga tatagttatt attgagaaac
    16081 caatgagagc ttattcagag caaagtgacc aaatgttgac agtaggtaat ggttgggaag
    16141 taatcgatga ggatcaatta gatgagcttg ctaaacagca agaacaagat gattcagaat
    16201 cacgacaagt tgatccaagg cttcaaaaat tacaacaatt atatgataaa gagcaataag
    16261 tagtttaatt aatgatataa tgttgttaat taaactaaca tttagtattt ttcgtttata
    16321 atctaaggag gatatatcat ggcagtacca aaaagaagaa cttctaaaac tagaaaaaac
    16381 aaacgtcgta cgcatttcaa aatttcagta ccaggtatga ctgaatgccc aaactgtggc
    16441 gaatacaaat tatcacaccg tgtatgtaaa aactgtggtt cttacaatgg cgaagaagta
    16501 gcagctaaat aattttagtt actcaatata aaaagtcccg cttaaaatga ttgttttaag
    16561 tgggactt
//
LOCUS       DAOBBH010000041        16392 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.041, whole genome shotgun sequence.
ACCESSION   DAOBBH010000041 DAOBBH010000000
VERSION     DAOBBH010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16392
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.041"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            24..965
                     /gene="pde2"
                     /locus_tag="P6L01_000997"
     CDS             24..965
                     /gene="pde2"
                     /locus_tag="P6L01_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pApA hydrolase Pde2"
                     /protein_id="HDP3030081.1"
                     /translation="MISTLNEIMKCIEDNDTIIIHRHVRPDPDAYGSQLGLKYYIQQK
                     FPQKQVFAVGEAESSLSFIGELDNIDDKTYQDALVIVCDTANAPRIDDERYSTGSKLI
                     KIDHHPAVDQYGDINLVNTNASSTSEIIYDLISHFNDEAIVNKDIASVLYLGIVGDTG
                     RFLFNNTSEHTMEIAGKLIGHDIDHNALLNKMMEKDPKMLPFQGYVLQHFELMDDGFC
                     QVKITEDVLEQFGIQPNEASQFVNTIADIKGLKIWVFAVDEGSEIRCRLRSKGQLIIN
                     DIAQDFGGGGHPNASGVSVNSWDEFEQLATALRTKLN"
     gene            986..4183
                     /locus_tag="P6L01_000998"
     CDS             986..4183
                     /locus_tag="P6L01_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDP3030082.1"
                     /translation="MVAYLNIHTAYDLLNSSLKIEDAVRLAVSENVDALAITDTNVLY
                     GFPKFYDACIANNIKPIFGMTIYVTNGLNTVETVVLAKNNDGLKDLYQLSSEIKMNSM
                     ENVSFELLQQFSSNLIIIFKNVADEHRDIVQVFDSHEDTYLDHQSVLVQGIKHVWIQN
                     VCYQTRQDADTISALAAIRDNAKLDLIHDQEDFGAHFLTEKEIKQLDINQEYLTQVDV
                     IAQKCNAELKYHQSLLPQYQTPNDESAKKYLWRVLVTQLKKLELNYDVYLERLKYEYK
                     VITNMGFEDYFLIVSDLIHYAKTNDVMVGPGRGSSAGSLVSYLLGITTIDPIKFNLLF
                     ERFLNPERVTMPDIDIDFEDTRRERVIQYVQEKYGELHVSGIVTFGHLLARAVARDVG
                     RIMGFDEVTLNEISSLIPHKLGITLDEAYQIDGFKKFVHRNHRHERWFSICKKLEGLP
                     RHTSTHAAGIIINDHPLYEYAPLTKGDTGLLTQWTMTEAERIGLLKIDFLGLRNLSII
                     HQILIQVKKDLGINIDIEKIPFDDQKVFELLSQGDTTGIFQLESDGVRSVLKKLKPEH
                     FEDIVAVTSLYRPGPMEEIPTYITRRHDPSKVQYLHPHLEPILKNTYGVIIYQEQIMQ
                     IASTFANFSYGEADILRRAMSKKNRAVLESERQHFIEGAKQNGYHEDISKQIFDLILK
                     FADYGFPRAHAVSYSKIAYIMSFLKVHYPNYFYANILSNVIGSEKKTAQMIEEAKKQG
                     ITILPPNINESHWFYKPSQEGIYLSIGTIKGVGYQSVKVIVDERYQNGKFKDFFDFAR
                     RIPKRVKTRKLLEALILVGAFDAFGKTRSTLLQAIDQVLDGDLNIEQDGFLFDILTPK
                     QMYEDKEELPDALISQYEKEYLGFYVSQHPVDKKFVAKQYLTIFKLSNAQNYKPILVQ
                     FDKVKQIRTKNGQNMAFVTLNDGIETLDGVIFPNQFKKYEELLSHNDLFIVSGKFDHR
                     KQQRQLIINEIQTLATFEEQKLAFAKQIIIRNKSQIDMFEEMIKATKENANDVVLSFY
                     DETIKQMTTLGYINQKDSMFNNFIQSFNPSDIRLI"
     gene            4633..5862
                     /locus_tag="P6L01_000999"
     CDS             4633..5862
                     /locus_tag="P6L01_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDP3030083.1"
                     /translation="MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPC
                     KDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGIN
                     GVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQ
                     HGTAIVTMAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRG
                     AIFEGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMA
                     DNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALEVEAT
                     HINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIE
                     VDPQDVYDKTMKLTDLQ"
     gene            6057..6914
                     /locus_tag="P6L01_001000"
     CDS             6057..6914
                     /locus_tag="P6L01_001000"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDP3030084.1"
                     /translation="MFKDFFNRTKKKKYLTVQDSKNNDVPAGIMTKCPKCKKIMYTKE
                     LAENLNVCFNCDHHIALTAYKRIEAISDEGSFTEFDKGMTSANPLDFPSYLEKIEKDQ
                     QKTGLKEAVVTGTAQLDGMKFGVAVMDSRFRMGSMGSVIGEKICRIIDYCTENRLPFI
                     LFSASGGARMQEGIISLMQMGKTSVSLKRHSDAGLLYISYLTHPTTGGVSASFASVGD
                     INLSEPKALIGFAGRRVIEQTINEKLPDDFQTAEFLLEHGQLDKVVHRNDMRQTLSEI
                     LKIHQEVTK"
     gene            6914..7858
                     /locus_tag="P6L01_001001"
     CDS             6914..7858
                     /locus_tag="P6L01_001001"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HDP3030085.1"
                     /translation="MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERET
                     KKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLN
                     GRAVTVIGQQRGKDTKDNIYRNFGMAHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAY
                     PGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTY
                     SVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDVEQQA
                     LAIKSAFVAQLDSLESLSRDEIANDRFEKFRNIGSYIE"
     gene            8127..9095
                     /gene="pfkA"
                     /locus_tag="P6L01_001002"
     CDS             8127..9095
                     /gene="pfkA"
                     /locus_tag="P6L01_001002"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDP3030086.1"
                     /translation="MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGL
                     LNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGG
                     DGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
                     SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHS
                     IVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDL
                     LMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI"
     gene            9117..10874
                     /gene="pyk"
                     /locus_tag="P6L01_001003"
     CDS             9117..10874
                     /gene="pyk"
                     /locus_tag="P6L01_001003"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDP3030087.1"
                     /translation="MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHK
                     GRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPE
                     KFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNL
                     PGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFP
                     KIENQEGIDNIAEILEVSDGLMVARGDMGVEIPPEKVPMVQKDLIRQCNKLGKPVITA
                     TQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSA
                     EAAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYR
                     PHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVSNGDLI
                     IITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDK
                     VIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNM
                     LVTIDAAQGKIFEGYANVL"
     gene            complement(11222..12583)
                     /locus_tag="P6L01_001004"
     CDS             complement(11222..12583)
                     /locus_tag="P6L01_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDP3030088.1"
                     /translation="MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILL
                     TYIIIGFMLFMFMRGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITGM
                     AEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATIIG
                     LIVVGFVMILFAFKTQFGHASFTNLYEHGIFAKGASGFFMSFQMALFSFVGIEMIGVT
                     AGETKDPVKTIPKAINSVPIRILIFYVGALAVIMSIIPWQQVDPDNSPFVKLFALIGI
                     PFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIFA
                     SSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKNPDLHKNA
                     TYKLLGGKYMGYLIFVFFIFVFGLLFINVDTRRAIYFIPIWFILLAFMYLRYKRIAAK
                     SNK"
     gene            12931..14052
                     /locus_tag="P6L01_001005"
     CDS             12931..14052
                     /locus_tag="P6L01_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HDP3030089.1"
                     /translation="MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEE
                     VIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYI
                     AHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYM
                     LRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPL
                     HGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSR
                     QITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSR
                     SAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK"
     gene            14101..15369
                     /gene="icd"
                     /locus_tag="P6L01_001006"
     CDS             14101..15369
                     /gene="icd"
                     /locus_tag="P6L01_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDP3030090.1"
                     /translation="MTAEKITQGTEGLNVPNEPIIPFIIGDGIGPDIWKAASRVIDAA
                     VEKAYNGEKRIEWKEVLAGQKAFDTTGEWLPQETLDTIKEYLIAVKGPLTTPIGGGIR
                     SLNVALRQELDLFTCLRPVRWFKGVPSPVKRPQDVDMVIFRENTEDIYAGIEFKEGTT
                     EVKKVIDFLQNEMGATNIRFPETSGIGIKPVSKEGTERLVRAAIQYAIDNNRKSVTLV
                     HKGNIMKFTEGSFKQWGYDLALSEFGDQVFTWQQYDEIVENEGRDAANAAQEKAEKEG
                     KIIIKDSIADIFLQQILTRPAEHDVVATMNLNGDYISDALAAQVGGIGIAPGANINYE
                     TGHAIFEATHGTAPKYAGLNKVNPSSVILSSVLMLEHLGWQEAADKITDSIEDTIASK
                     VVTYDFARLMDGAEEVSTSAFADELIKNLK"
     gene            15759..>16392
                     /locus_tag="P6L01_001007"
     CDS             15759..>16392
                     /locus_tag="P6L01_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDP3030091.1"
                     /translation="MSQKVLVVDDEHSIVTLLKYNLETAGYEVVVAFDGDEALEKVES
                     EQPDLIILDVMLPKKDGIDVCKTVRTNKNLVPILMLTAKNDEFDRVLGLELGADDYMT
                     KPFSPREVVARVKAILRRSQFVKEVEEIDDDITIGSIRIRPDYFEVYKHNELLELTPK
                     EFELLLYLIERQGRVITREHMLNTVWNYEFAGDSRIVDVHISHLRDKLEDN"
BASE COUNT         5844 a         2258 c         3178 g         5112 t
ORIGIN      
        1 tttctaaaaa agggagtatg aaaatgatta gtactttgaa tgaaattatg aaatgtatcg
       61 aagacaacga tacaattatt atacacagac atgttagacc agatccagat gcatatggtt
      121 cacaacttgg tttaaaatac tatattcagc agaaatttcc gcaaaagcaa gtatttgcag
      181 tgggtgaagc ggaatcatca ttaagtttta ttggagagtt ggataacatt gatgataaaa
      241 catatcaaga tgcgcttgta attgtatgtg atactgccaa tgctccacga attgacgatg
      301 aacgatatag tacaggtagt aaacttatta aaatagatca tcatcctgca gttgatcagt
      361 atggtgatat taatttagtt aatacgaacg cgtcatctac aagtgaaatc atttatgatt
      421 taatctcaca ttttaatgat gaagcaattg ttaataaaga tatcgcgagt gttttatacc
      481 ttggtatcgt cggtgatact gggcgattcc tttttaacaa tacctcagaa catactatgg
      541 aaattgctgg aaagttaatt gggcatgata ttgatcataa tgcgctatta aataaaatga
      601 tggagaagga cccaaaaatg ttgccgtttc aaggttatgt tttacaacat ttcgaactta
      661 tggatgatgg attctgccaa gttaaaataa ctgaagatgt attggagcaa ttcggtattc
      721 agccaaatga agcatctcag tttgttaata caattgctga catcaaaggt ttgaaaatat
      781 gggtgtttgc agttgatgag ggtagtgaaa taagatgtcg cttacgctct aaagggcaat
      841 tgattattaa tgatattgcg caagattttg gtggcggtgg ccatccgaat gcgtcaggag
      901 tttcagtgaa cagctgggat gaatttgagc aacttgctac agctttacgc acaaaactta
      961 actaatagaa aggagccatt caatcatggt ggcatattta aatattcata cggcttatga
     1021 tttgttaaat tcaagcttaa aaatagaaga tgccgtaaga cttgctgtgt ctgaaaatgt
     1081 tgatgcactt gccataactg acaccaatgt attgtatggt tttcctaaat tttatgatgc
     1141 atgtatagca aataacatta aaccgatttt tggtatgaca atatatgtga caaatggatt
     1201 aaatacagtc gaaacagttg ttctagctaa aaataatgat ggattaaaag atttgtatca
     1261 actatcatcg gaaataaaaa tgaattcaat ggaaaatgtt tcatttgaac tattgcaaca
     1321 attttcatcg aatttgatta tcatttttaa aaatgttgca gacgaacatc gtgacattgt
     1381 tcaagttttt gattcgcatg aagatacgta tttagatcat caaagtgttt tagttcaggg
     1441 tataaagcac gtatggattc aaaatgtttg ttaccaaaca cgtcaagatg ccgatacgat
     1501 ttctgcatta gcagctatta gagacaatgc aaaattagac ttaattcatg atcaagaaga
     1561 ttttggtgca cattttttaa ctgaaaagga aattaaacaa ttagatatta accaagaata
     1621 tttaacgcag gttgatgtta tagctcaaaa gtgtaatgca gaattaaaat atcatcaatc
     1681 tctacttcct caatatcaga cacctaacga tgaatcagct aaaaaatatt tgtggcgtgt
     1741 cttagttaca caattgaaaa aattagaact taattatgac gtctatttag agcgattgaa
     1801 atatgagtat aaagttatta ctaatatggg ttttgaagat tatttcttaa tagtaagtga
     1861 tttaatccat tatgcgaaaa cgaatgatgt gatggtaggt ccaggtcgtg gttcttcagc
     1921 tggctcactg gtcagttatt tactaggtat tacaacgatt gatcctatta aattcaatct
     1981 attatttgaa cgttttttaa acccagaacg tgtaacaatg cctgatatag atattgactt
     2041 tgaagataca cgccgagaaa gggttattca gtacgtccaa gaaaaatatg gcgagctaca
     2101 tgtatctgga attgtgactt tcggtcatct gcttgcaaga gcggttgcta gggatgttgg
     2161 acgaattatg gggtttgatg aagttacatt aaatgaaatt tcaagtttaa tcccacataa
     2221 attaggaatt acacttgatg aagcatatca aattgacggt tttaaaaaat ttgtacatcg
     2281 aaaccatcga catgaacgct ggttcagtat ttgtaaaaag ttagaaggtt taccaagaca
     2341 tacatctaca catgcggcag gaattattat taatgaccat ccattatatg aatatgcccc
     2401 tttaacgaaa ggggatacag gattattaac gcaatggaca atgactgaag ccgaacgaat
     2461 tgggttatta aaaatagatt ttctagggtt gagaaactta tcgattattc atcaaatctt
     2521 aatacaagtc aaaaaagatt taggtattaa tattgatatc gaaaagattc cgttcgatga
     2581 tcaaaaagtg tttgaattgt tgtcgcaagg agatacgact ggcatattcc aattagagtc
     2641 agacggtgta agaagtgtat taaaaaaatt aaagccggaa cactttgaag atattgttgc
     2701 tgtaacttct ttgtatagac caggtccaat ggaagaaatt ccaacttaca ttacaagaag
     2761 acatgatcca agcaaagttc aatatttaca tccacattta gaacctatat taaaaaatac
     2821 ttacggtgtt attatttatc aagagcaaat tatgcaaata gcgagcacat ttgcaaactt
     2881 cagttatggt gaagcggata ttttaagaag agcaatgagt aaaaaaaata gagctgttct
     2941 tgaaagtgag cgtcaacatt ttatagaagg tgcaaagcaa aatggttatc acgaagacat
     3001 tagtaagcaa atatttgatt tgattctgaa atttgctgat tatggttttc ctagagcaca
     3061 tgctgtcagc tattctaaaa ttgcatacat tatgagcttt ttaaaagtcc attatccaaa
     3121 ttatttttac gcaaatattt taagtaatgt tattggaagt gagaagaaaa ctgctcaaat
     3181 gatagaagaa gcaaaaaaac aaggtatcac tatattgcca ccgaacatta acgaaagtca
     3241 ttggttttat aaaccttccc aagaaggcat ttatttatca attggtacaa ttaaaggtgt
     3301 tggttatcaa agtgtgaaag tgattgttga tgaacgttat cagaacggca aatttaaaga
     3361 tttctttgat tttgctagac gtataccgaa gagagtcaaa acgagaaagt tacttgaagc
     3421 actgatttta gtgggagcgt ttgatgcttt tggtaaaaca cgttcaacgt tgttgcaagc
     3481 tattgatcaa gtgttggatg gcgatttaaa cattgaacaa gatggttttt tatttgatat
     3541 tttaacgcca aaacagatgt atgaagataa agaagaattg cctgatgcac ttattagtca
     3601 gtacgaaaaa gaatatttag gattttatgt ttcgcaacac ccagtagata aaaagtttgt
     3661 tgccaaacaa tatttaacga tatttaaatt gagtaacgcg cagaattata aacctatatt
     3721 agtacagttt gataaagtta aacaaattcg aactaaaaat ggtcaaaata tggcattcgt
     3781 cacattaaat gatggcattg aaactttaga tggtgtgatt ttccctaatc agtttaaaaa
     3841 gtacgaagag ttgttatcac ataatgactt gtttatagtt agcgggaaat ttgaccatag
     3901 aaagcaacaa cgtcaactaa ttataaatga gattcagaca ttagccactt ttgaagaaca
     3961 aaaattagca tttgccaaac aaattataat tagaaataaa tcacaaatag atatgtttga
     4021 agagatgatt aaagctacga aagagaatgc taatgatgtt gtgttatcct tttatgatga
     4081 aacgattaaa caaatgacta ctttaggcta tattaatcaa aaagatagta tgtttaataa
     4141 ttttatacaa tcctttaacc ctagtgatat taggcttata taacttttat caactaataa
     4201 attatgatat agtaaactga tggttagata ttttttaacc atcagtttgc gtttatatta
     4261 gttttttatg cttattattt ttatgagttt cactttacat tattgattaa tccataagaa
     4321 taattagcac aaaaagcagt atacataaat tgagtaaaga attttgtcga tatatcgtgc
     4381 ggaaaaataa taactttttg atatattggt ttttgctatc cattataata attaaaggta
     4441 tatctttaga tagtatggtt taatgtatct ttataaatat tattttatta tcgtttatcg
     4501 ctatttgtgt tacataatca aaaccataaa ttcttactca ttcagattta cccaatattt
     4561 ttacttttat aatgtaatgc gttttatcca agttattttt taaaaataaa tattgaatta
     4621 ggggctgatt tcatgtcatt aagagatgaa gcattggaaa tgcacaaacg taatcaaggt
     4681 aaattagaag ttaaaccaaa tgtaaaagtt actaataaag aggaattaag tttagcatac
     4741 tcacctggcg ttgctgaacc gtgtaaagat atttatgaag ataaaagaaa agtatatgat
     4801 tacacaatta aaggaaatac agttgcagtt attactgatg gaacagcggt attaggttta
     4861 ggtaacattg gacctgaagc aagtattcct gtaatggaag gtaaagcagt attattcaaa
     4921 agcttcgctg gtatcaatgg ggtgcctatt gcgttaaata caactgatac cgaagaaatc
     4981 attaaaacag ttaagttgtt agaacctaat tatggtggta ttaatttaga ggatatttcg
     5041 gcaccacgtt gttttgaaat tgaagaacga ttgaaaaaag aaactaatat tccggtattc
     5101 catgacgatc aacatggtac agcaattgta acaatggcag gtttggtaaa tgcattgaga
     5161 gttgttaaca aagatattgc taaaataaaa gttgtactaa atggtgctgg tgcagcagga
     5221 atagccattg ttaaattact atacgcgtat ggtgtaagaa atatggttat gtgtgactca
     5281 agaggcgcaa tttttgaagg acgttcatat ggtatgaatc ctacgaaaga tgttgtagct
     5341 aaatggacaa ataaagataa gattgaaggg tctttagaag aagtcgtaaa agacgcagat
     5401 gtatttatcg gagtttctgt agctaatgcg ctgtcacaag atatggttaa gagtatggca
     5461 gataatccaa ttatatttgc aatggctaat ccaaatcctg aaataatacc tgatgatgcc
     5521 aaagcggcag gtgcacgagt tgttggtaca ggacgttcag actatcctaa ccaaattaat
     5581 aatgtattag ctttccctgg tatttttaga ggtgcattag aggttgaagc tacacatata
     5641 aatgaagaaa tgaaaaaggc agctgtagaa gcgattgctg atttaatcga tagttctgaa
     5701 ttaaatgaag actactgtat cccaggaccg tttgataaac gtgtagcgcc atcagttgct
     5761 cgtaatgttg ctaaagcggc aatggaatct ggagtagcta ggattgaagt tgatccgcaa
     5821 gatgtgtatg ataaaacaat gaaacttaca gatttacaat aaaaataatt aacgtttaat
     5881 taaattgctt aaaagttagc aataaagagt cattaaaatg atgaaaatga aagtttatga
     5941 taaacattca acagtcaaac gaatataaat caaataaatt taaacccgtt tttaactggt
     6001 caagttcagt ttaaggctct aaatggttag aacagaggtt atttggaggt tttcctatgt
     6061 ttaaagattt ttttaatcga acaaagaaaa agaaatatct tacagtacaa gactctaaaa
     6121 ataatgatgt gcctgcaggt attatgacta agtgtccaaa gtgtaagaaa attatgtaca
     6181 caaaagaatt agctgaaaat ttaaatgtgt gctttaattg tgatcatcat attgctttaa
     6241 ctgcgtataa acgtatagaa gcaatttctg atgaaggatc atttacagaa ttcgataagg
     6301 gaatgacctc tgcgaatcca ttagattttc caagttattt agaaaaaatt gaaaaggacc
     6361 aacaaaagac aggtcttaaa gaagcagttg tgactggtac agcgcaacta gatggtatga
     6421 aatttggcgt tgctgtcatg gattcacgtt ttagaatggg aagtatggga tcggttatcg
     6481 gtgaaaagat atgtcgcatc attgattact gcactgagaa ccgtttacca tttattcttt
     6541 tctctgcaag tggtggtgca cgtatgcaag aaggtattat ttccttgatg caaatgggta
     6601 aaaccagtgt atctttaaaa cgtcattctg acgctggact attatatata tcatatttaa
     6661 cacatccaac tactggtggt gtatctgcaa gttttgcatc agttggtgat ataaatttaa
     6721 gtgagccaaa agcgttgata ggttttgcag gtcgtcgagt tattgaacag acaataaacg
     6781 aaaaattgcc agatgatttc caaactgcag aatttttatt agagcatgga caattggata
     6841 aagttgtaca tcgtaatgat atgcgtcaaa cattgtctga aattctaaaa atccatcaag
     6901 aggtgactaa ataatgttag attttgaaaa accacttttt gaaattcgaa ataaaattga
     6961 atctttaaaa gaatctcaag ataaaaatga tgtggattta caagaagaaa ttgacatgct
     7021 tgaagcgtca ttggaacgag aaactaaaaa aatatataca aatctaaaac catgggatcg
     7081 tgtgcaaatt gcgcgtttgc aagaaagacc tacgacccta gattatattc catatatctt
     7141 tgattcgttt atggaactac atggtgatcg taattttaga gatgatccag caatgattgg
     7201 tggtattggc tttttaaatg gtcgtgctgt tacagttatt ggacaacaac gtggaaaaga
     7261 tacaaaagat aatatttatc gaaattttgg tatggcgcat ccagaaggtt atcgaaaagc
     7321 attacgttta atgaaacaag ctgaaaaatt caatcgtcct atctttacat ttatagatac
     7381 aaaaggtgca tatcctggta aagctgctga agaacgtgga caaagtgaat ctatcgcaac
     7441 aaatttgatt gagatggctt cattaaaagt accagttatt gcgatagtca ttggtgaagg
     7501 tggcagtgga ggtgctctag gtattggtat tgccaataaa gtattgatgt tagagaatag
     7561 tacttactct gttatatctc ctgaaggtgc agcggcatta ttatggaaag acagtaattt
     7621 ggctaaaatt gcagctgaaa caatgaaaat tactgcccat gatattaagc aattaggtat
     7681 tatagatgat gtcatttctg aaccacttgg cggtgcacat aaagatgttg aacagcaagc
     7741 tttagctatt aaatcagcgt ttgttgcaca gttagattca cttgagtcat tatcacgtga
     7801 tgaaattgct aatgatcgct ttgaaaaatt cagaaatatc ggttcttata tagaataatc
     7861 aacttgagca tttttatgtt aaatcgatac tgggttttac cataaattga agtacattaa
     7921 aacaataatt taatatttag atactgaatt ttaactaaga ttagtagtca aaattgtggc
     7981 tactaatctt tttttaatta agttaaaata aaattcaata tttaaaacgt ttacatcaat
     8041 tcaatacatt agttttgatg gaatgacata tcaatttgtg gtaatttaga gttaaagata
     8101 aatcagttat agaaaggtat gtcgtcatga agaaaattgc agttttaact agtggtggag
     8161 attcacctgg aatgaatgct gccgtaagag cagttgttcg tacagcaatt tacaatgaaa
     8221 ttgaagttta tggtgtgtat catggttacc aaggattgtt aaatgatgat attcataaac
     8281 ttgaattagg atcagttggg gatacgattc agcgtggagg tacattcttg tattcagcaa
     8341 gatgtccaga gtttaaggag caagaagtac gtaaagttgc aatcgaaaac ttacgtaaaa
     8401 gagggattga gggccttgta gttattggtg gtgacggtag ttatcgcggt gcacaacgca
     8461 tcagtgagga atgcaaagaa attcaaacta tcggtattcc tggtacgatt gacaatgata
     8521 tcaatggtac tgattttaca attggatttg acacagcttt aaatacgatt attggcttag
     8581 tcgacaaaat tagagatact gcgtcaagtc acgcacgaac atttatcatt gaagcaatgg
     8641 gccgtgattg tggagatcta gcattatggg ctggattatc agttggtgct gagacaattg
     8701 tagttccaga agtgaaaaca gatattaaag aaatagctga taaaattgaa caaggtatta
     8761 aacgtggtaa gaaacactca atcgttcttg tagcagaagg ttgcatgact gcgcaagatt
     8821 gtcaaaaaga attatcacaa tacatcaatg ttgataatag agtgtctgtg ttaggtcacg
     8881 ttcaacgtgg tggtagccca acaggtgcgg atagagtttt agcatcacgt ttaggtggat
     8941 atgcggtaga cttattaatg caaggtgaaa cagctaaggg tgttggaatt aagaacaata
     9001 aaattgtagc aacatctttt gatgaaattt ttgatggtaa agatcataaa tttgattata
     9061 gtctatatga acttgctaac aagttatcta tataagattt caggaggaat tataaaatga
     9121 gaaaaactaa aattgtatgt acaattggac cagcttcaga atcagaagaa atgattgaga
     9181 aattaatcaa tgctggtatg aacgttgcac gattaaactt ttcacatggt agtcatgaag
     9241 agcataaagg tagaattgat acaattcgta aagtagctaa aagattagac aaaattgtag
     9301 caattttatt agatacgaaa ggtccagaaa ttcgtacgca taatatgaaa gacggtatca
     9361 ttgaacttga acgtggtaac gaagttattg ttagcatgaa tgaagttgaa ggaacacctg
     9421 aaaagttctc agtaacatat gaaaacttaa ttaacgatgt tcaagtaggt tcatacattt
     9481 tacttgatga tggcttaatt gaattacaag ttaaagatat tgaccatgct aaaaaagaag
     9541 ttaaatgtga tattttaaac tctggtgagc ttaaaaacaa aaaaggtgtt aacttacctg
     9601 gtgtaagagt aagtttacct ggtattacag aaaaagatgc tgaagatatc cgtttcggta
     9661 ttaaagaaaa tgttgacttc attgcagcaa gtttcgtacg tcgtcctagt gatgttttag
     9721 aaattcgtga aattttagaa gaacaaaaag ctaacatttc agtattccct aaaattgaaa
     9781 accaagaagg tattgataat attgcggaaa ttcttgaagt gtctgatggt ttaatggttg
     9841 cacgtggtga catgggtgtt gaaattccac ctgaaaaagt accaatggtt caaaaagatt
     9901 taatcagaca atgtaacaaa ttaggtaaac cagttattac agctacacaa atgttagatt
     9961 ctatgcaacg taacccacgt gctacacgtg cagaagctag tgacgttgcc aacgcaatct
    10021 atgatggtac agatgcagta atgttatctg gtgaaactgc tgctggttta tatcctgaag
    10081 aagctgttaa aacaatgaga aatattgctg tatcagctga agcagcccaa gattataaaa
    10141 agttattgtc agatcgtact aaattagttg aaacttcatt agtgaatgct atcggtattt
    10201 cggttgcaca tacagcttta aacttaaatg ttaaagcaat tgtagctgct actgaaagtg
    10261 gttcaacggc acgtactatc tctaaatatc gtccacattc agacattatt gcggtgactc
    10321 caagtgaaga aacagcacgt caatgttcaa ttgtttgggg agttcaacct gtagttaaaa
    10381 aaggacgtaa gagtacagat gcattgttaa acaatgcagt tgcaacagct gttgaaactg
    10441 gtagagtatc taatggtgat ttaatcatta ttactgctgg tgtaccaact ggtgaaactg
    10501 gaactactaa tatgatgaaa atccacctag ttggtgacga aattgctaat ggtcaaggta
    10561 ttggacgtgg atcagttgtt ggtactacgt tagttgctga aactgttaaa gatttagaag
    10621 gtaaagattt atctgacaaa gttatcgtta ctaactcaat cgatgaaacg tttgtacctt
    10681 atgtagaaaa agctttagga ttaattacag aagaaaatgg tattacatca ccaagtgcaa
    10741 ttgttggttt agaaaaaggt attccaacag ttgtaggtgt agaaaaagct gttaaaaaca
    10801 taagcaataa catgttagtt acgattgatg ctgctcaagg taaaatcttt gaaggatatg
    10861 caaacgtact ataatttata aaaaaacgtc tttccattta tcaacaatgg aaaggcgttt
    10921 tttggttcat ctggtatttt atgacgtaat taataggtta tttgataatg atagtgtatg
    10981 aatggcaatc tatataaatg tttatatctt ttatacatgt acattatcac cttcaaactt
    11041 tcactcatat tactttggaa atttattata aaatagaagt atggatgtat ttctgaaatg
    11101 atacattatt aaatagatga gaaagtaaaa gttttgagcc aagtacgcaa tttaatatta
    11161 taagttgcat ataaaaaccc ctgttgaact ttagtatatt caacaggggt aaaacttgtt
    11221 gttatttatt tgatttagca gcgatacgtt tatatcttaa gtacataaat gctaaaagta
    11281 taaaccaaat cggaataaaa taaattgcac gtcttgtatc aacattaata aataataacc
    11341 cgaacacaaa aatgaagaat acaaatatta agtagcccat atatttacca cctaatagtt
    11401 tgtacgtagc atttttatgt agatctgggt ttttacgact ataattgata tatgcaatga
    11461 taatcagacc ccatacaact aaaaataaca ctgtagagat ggtagtcaca tacgtaaata
    11521 cttttgtcgc atctgggaaa atatagttta gtaatgctgc cacaagtaat aatgctgatg
    11581 aagcaaagat tgcaacatgt ggaacgccat atttattcgt cttagaaaag ttcggaggtg
    11641 cttgttgttg acttgataaa ccgaaaagca tacggctatt tgagaatata ccactgttac
    11701 atgatgaagc agcagcggtt aatactacaa aattaatcaa gcccgcagca aacggaattc
    11761 cgatcaatgc gaataatttt acgaatggac tgttatcagg atcaacttgc tgccaaggga
    11821 taatagacat gataaccgct aacgcaccaa cgtaaaatat taaaattcta atgggtacac
    11881 tgttaattgc ctttggaatt gttttaactg gatcttttgt ttccccagct gtaacaccaa
    11941 tcatttcaat tcctacaaat gagaataatg ccatttggaa agacataaag aatccagaag
    12001 cacctttagc gaatatgccg tgttcatata aatttgtgaa actggcatgc ccgaattgag
    12061 ttttaaatgc aaatagaatc atgacgaaac caactactat taaaccaata attgtcgcta
    12121 ttttaatgat agagaaccaa aattctaatt ctccgaaaag tcttgcgcta agtaggttga
    12181 atgacattaa taacagtaca caaaatagtg cacttatcca gtttggaatt tctgggaacc
    12241 aaaagctaac atattttgcc acagccgtta cttcagccat acctgtaata atccaacaga
    12301 accagtatgt ccatccggta acaaatcctg caaaaggccc aatatatgta ttggttacat
    12361 ctgcgaaaga tttaaattca gtattctgta taatgatttc tcctaaacct cgcataaaca
    12421 taaataacat aaatcctata atgatgtatg ttaatagaat tgaagggccg gttaatgcaa
    12481 tcgtttgacc agcacctaag aataagcctg taccaattgc accgccaatt gcaattaatt
    12541 gtatgtggcg attgctcagt tccctttgta atttttcagc cataatacat ctcccttaaa
    12601 tatagatatg tttattatgc acttatattg agatatatac aattattttc ggtaaaaatg
    12661 tgtaaaattc catgttaata tactttggtt tttataatca tatataataa ccaattgaaa
    12721 atttaattct attgtaaaat tcatggatta ttcacatctt gaaaaagctt taatggtgct
    12781 atttgtggct attctgtgac atttacatag atttacaaaa aaattgttgc acatataatg
    12841 ccagttttta tatttcacaa acgaaatgcg tttagctata atattagttg aaagccattt
    12901 cataaagaaa cagtaaaggg gaaatttatc atggcagaat tacaaagagg tttagaaggg
    12961 gttatcgcag cggagactaa aataagttca attattgaaa gtcaattgac ttatgccggc
    13021 tatgatattg atgatctagc tgaaaatgcg caatttgaag aagttatttt cctattatgg
    13081 aactatagat tgccaaacga agaagagctt gctcatctaa aagggaaatt aaatcaatac
    13141 atgacattaa atcctcgtgt gtatacacat tttgaggagt atgttacaga tcacgtgcat
    13201 ccaatgacag cattacgtac gtcattatca tatattgcac atttcgatcc tgatgctgaa
    13261 aatgaatcag atgaaaatcg ttatgaaaga gcaatgcgta tacaggctaa agtagcatca
    13321 ttagttacag cgtttgctcg agtaagacaa gataaagaac cacttaagcc taatcctgac
    13381 ttaagttatg cggcaaactt cctatatatg ttacgtgggg aattaccaac agatatagaa
    13441 gtagaagcct tcaataaagc acttatttta cacgctgatc atgagttgaa cgcatctgca
    13501 tttacagcac gttgtgcggt atcatcattg tcagatatgt actcaggtat tgtagcagct
    13561 gtaggttctc tgaaagggcc attacatggt ggtgcaaacg aacaagttat gacgatgtta
    13621 tctgagattg ggtcaattga aaatgttgat gcttacttag atgaaaaatt tgctaataaa
    13681 gataaagtaa tgggctttgg tcatcgtgta tataaagatg gtgatcctag agcgaaatat
    13741 ttaagagaaa tgagccgtca aattacgaaa gacgctggtc gtgaagaatt atttgaaatg
    13801 tcagtgaaaa tggaaaaacg tatggcagaa gaaaaaggat taattcctaa tgttgatttt
    13861 tatagtgcga gtgtttatca ctgtatggaa atacctcatg acttattcac gccaatcttt
    13921 gctgtaagtc gttctgcagg atggattgct catattttag aacaatataa agataataga
    13981 attatgcgtc ctagagcgaa atatattggc gaaacgaatc gtaagtatat cccgcttgaa
    14041 gaaagaaaat aatcaataca aattaaaaat gaagatgtaa aatttggagg taaaataact
    14101 atgactgcag aaaaaattac tcaaggaact gaaggattaa acgtacctaa tgaaccaatt
    14161 atcccattta ttatcggtga tggaattgga ccggatattt ggaaggcagc aagccgagtt
    14221 atagatgctg ctgttgagaa agcctataat ggcgaaaaac gcattgaatg gaaagaagtg
    14281 ctagctggcc aaaaagcatt tgatacaact ggtgaatggt tacctcaaga aacacttgat
    14341 acaattaaag aatatttaat tgctgttaaa ggacctttaa caacaccaat tggtggtggt
    14401 attagatcat taaatgtggc tttacgccaa gaattagatt tatttacttg cttaagaccg
    14461 gtacgttggt ttaaaggagt accatcacct gttaaacgtc cacaagatgt tgatatggtt
    14521 attttccgtg aaaatactga agacatttat gctggtattg aatttaaaga aggtacaaca
    14581 gaagttaaaa aggtaattga cttcttacaa aacgaaatgg gtgcgacaaa cattcgattc
    14641 ccagaaactt caggtattgg tattaaacca gtttctaaag aaggaactga gcgattagtt
    14701 agagcagcta tacaatatgc tatcgataat aaccgtaaat cagttacttt agttcataaa
    14761 ggtaatatta tgaaatttac agaaggctca tttaagcagt ggggttacga tttagcatta
    14821 tctgaatttg gtgatcaagt attcacttgg caacaatatg acgaaattgt tgaaaatgaa
    14881 ggcagagatg ctgctaatgc tgctcaagaa aaagctgaaa aagaaggcaa gattatcatt
    14941 aaagattcta ttgctgacat tttcttacaa caaattttaa ctcgtccagc tgagcatgat
    15001 gttgtagcaa ctatgaactt gaatggtgac tatatttcag atgctttagc tgcacaagtt
    15061 ggtggtattg gtattgcgcc aggtgcaaac attaattatg aaacaggtca tgctattttt
    15121 gaagcaacac atggtacagc tccaaaatat gcaggtttaa ataaagtgaa tccatcttca
    15181 gtaattttaa gttctgtatt aatgttagaa catttaggat ggcaagaagc ggcagataag
    15241 attacagatt caattgaaga tacaattgct tcaaaagttg ttacttatga ctttgcccgt
    15301 ttaatggatg gtgctgaaga agtttctaca tcagcatttg cagatgaatt gattaaaaat
    15361 ttaaaataag caaaataaaa ttaggtgagt ctagcaactt taattgctag gttcgccttt
    15421 ttaaatgaat tgaagttggc aacaggatag aaataataaa gagtcaccaa tgattgatta
    15481 tgtagtgttt attacacatt agacaaagcg aatttgtaca taagcttagg aataaatgag
    15541 taaaactcat gcataggtag tgggatagaa atgatatttt tacaaaattt atttcgttgt
    15601 cccagagtaa gtcatgcaaa tgatagaagg aaatataaga agtattttat ttatatgtaa
    15661 agtaatgtaa aaaatgtgta aagataatgt gtaggatgtt atttttataa atagttcggt
    15721 tataatgtaa atataattat aagttaggga ggcataccat gtcgcaaaaa gtgttggtag
    15781 tagatgacga acattcaatc gtaacattac ttaaatataa cttagaaaca gctggttatg
    15841 aagttgttgt cgcatttgat ggtgatgagg ctttagaaaa ggtagaaagt gaacagccag
    15901 atttaattat tttagatgtt atgctaccta aaaaagatgg cattgacgta tgtaagactg
    15961 taagaacaaa taaaaattta gtccctatat taatgttaac ggcgaaaaat gatgagtttg
    16021 atcgggtatt aggtttagaa ttaggtgccg atgactatat gactaagcct ttttcaccta
    16081 gagaagttgt tgcccgtgtg aaagccattt taagacgttc tcaatttgta aaagaagtag
    16141 aagagattga cgatgatatt accatcggat cgatacgtat tagacccgat tattttgaag
    16201 tatataagca taatgagtta ttggagctaa caccgaaaga atttgaactc ttgctttatt
    16261 tgatagaaag acaaggcaga gtcattacga gagagcatat gttaaatacg gtatggaatt
    16321 atgaatttgc aggagactca agaattgttg atgtacatat cagtcattta agagataaat
    16381 tagaagataa tc
//
LOCUS       DAOBBH010000042        15757 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.042, whole genome shotgun sequence.
ACCESSION   DAOBBH010000042 DAOBBH010000000
VERSION     DAOBBH010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15757
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.042"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(35..184)
                     /locus_tag="P6L01_001008"
     CDS             complement(35..184)
                     /locus_tag="P6L01_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030092.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIV
                     FYEDK"
     gene            complement(731..1047)
                     /locus_tag="P6L01_001009"
                     /pseudo
     CDS             complement(731..1047)
                     /locus_tag="P6L01_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
     gene            complement(1040..2023)
                     /locus_tag="P6L01_001010"
     CDS             complement(1040..2023)
                     /locus_tag="P6L01_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDP3030093.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILR
                     DRLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGL
                     NKPNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFDFRTSRSSKIERPE
                     VRKSNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQ
                     EFPEQLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLK
                     KAIHDRGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
     gene            2449..3192
                     /locus_tag="P6L01_001011"
     CDS             2449..3192
                     /locus_tag="P6L01_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDP3030094.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NTRQNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPA
                     NLNDNDSDIEEKSEETEPKKGFWSRLFGS"
     gene            complement(3408..3593)
                     /locus_tag="P6L01_001012"
     CDS             complement(3408..3593)
                     /locus_tag="P6L01_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030095.1"
                     /translation="MFGDQRQEATKYVIKEGYQDIYFLNKNGEWYYFEVRSAWRGKHI
                     IRVKDGLLGWRKEIVTE"
     gene            3742..3837
                     /locus_tag="P6L01_001013"
     CDS             3742..3837
                     /locus_tag="P6L01_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDP3030096.1"
                     /translation="MNEILVHIMTTAISGCLVTLFGYWLHKRDKK"
     gene            complement(4052..4393)
                     /locus_tag="P6L01_001014"
     CDS             complement(4052..4393)
                     /locus_tag="P6L01_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDP3030097.1"
                     /translation="MKLAEAIKEQRELKRWSQEELANILKVSRQSVSKWESAKNYPSL
                     DILIAMSDLFGISLEHLIKGDARFKKVILEGNYRESNRAPSIVDFLYDFWWLFFPVAG
                     FLVWAIHSFMG"
     gene            complement(4480..5340)
                     /locus_tag="P6L01_001015"
     CDS             complement(4480..5340)
                     /locus_tag="P6L01_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDP3030098.1"
                     /translation="MTGETVVYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLR
                     LSFDELKKLSNYSPETRNINRFANDLDNVYKKMLNLTIRYEDDDVIEIFVLFNHYRIH
                     KREQYLEISTSSNLKHILNSITNNFTKFELKEMTRLKSTYSKNMFRLLKQYKHTGYLK
                     IHIDDFKNRLDIPKSYRMTDINKNVFKPIIIELGSIFNNLTINKIKAKKGRKIEWIEF
                     TFDAEKRIHNKRQPQMSKIDKSRQYVRREKTPKWLEERSYEKQPQKDYDPQLEKERED
                     FLKQLELNWE"
     gene            complement(5703..6203)
                     /locus_tag="P6L01_001016"
     CDS             complement(5703..6203)
                     /locus_tag="P6L01_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HDP3030099.1"
                     /translation="MYEIKTKNVGGWFHKEKQETGNIVITKTYFEKYTKQIKAAQMIL
                     DDYEWIKSGKSLKKSEKQNESLVNELTSVHMENEKLVEEFNDLAQRYNYLLSENEKKD
                     KELNYTLKLFNQVFKIIKSMMKEERYHTLINHIDNHLDNSKIREVMTIDNNDEQFFKK
                     KYQAQE"
     gene            complement(6270..6596)
                     /locus_tag="P6L01_001017"
     CDS             complement(6270..6596)
                     /locus_tag="P6L01_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDP3030100.1"
                     /translation="MEEEYGKENLLYATVHMDEITPHMHYGVIPITKDGRLSAKEVVG
                     NKKALTEFQDRFNTYINKQGYDLKRGISRQLTKEKHDQVSGYKQKTEYHKQMYMREKQ
                     IEDHLK"
     gene            complement(6536..6913)
                     /locus_tag="P6L01_001018"
     CDS             complement(6536..6913)
                     /locus_tag="P6L01_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDP3030101.1"
                     /translation="MSYSIIRVVKVKSKTNTRGIQRENKNYENIDIDLSKSYLNYDLV
                     NDTKFDFNKKIDEKIEQNYKGKRKIRTDSIKHIDGLITSDNVFLINYLKKRQNSFLSM
                     LKRSWKKNTVKKIFYMRQYIWMR"
     gene            complement(7151..7777)
                     /locus_tag="P6L01_001019"
     CDS             complement(7151..7777)
                     /locus_tag="P6L01_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3030102.1"
                     /translation="MIKLENVFVKKGNKNILDGCNFNFEKGKSYALVGESGAGKSTLL
                     NIIAGFEDVSQGSIYIEDKLLKKKVDFYRYTLGYLFQNFALLENQTISQNLDLALKFK
                     KNKKDNMNLKKEVLKKVGLDLDIKRIVSSLSGGEQQRVALARLILKDPKIILADEPTG
                     SLDTKNGKIVIDLLLKLLDENKTMIVVTHDLELAKRFDVIVNISELRS"
     gene            complement(7789..8100)
                     /locus_tag="P6L01_001020"
     CDS             complement(7789..8100)
                     /locus_tag="P6L01_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDP3030103.1"
                     /translation="MKRTLIIVTIVLLIIILSLLLVRNEHLDRFNPLLKEKVSYAKVP
                     KDTQDYRNITVYSKNGEKKSYKLDFLGNDPTKEYVKVNHKGKYVRSIEYITKDIPSFL
                     K"
     gene            complement(8104..10038)
                     /locus_tag="P6L01_001021"
     CDS             complement(8104..10038)
                     /locus_tag="P6L01_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HDP3030104.1"
                     /translation="MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNW
                     DKSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKEFFVFNSKRNNSDFIRNKTSLLT
                     PSDLLNREIKGKYYIIGEHFNVEELTSELKKAGLTSEVEYINRNILFFELVIDNDLLV
                     PFIFLGVLYLLYFLYDRGYNLKFYAIQKLHGFSTTRIIFHNIHIKIIYWLVLTTIFFI
                     ANILIIHIANLELDFLMFIRRFIVLLFVFACITTLLWLISYSLLLKLSIPLTLKGKKG
                     YKFSIFMGTFTKCIMVLILSFLLAQNLNAYTKLKNIYDSEKIWQKLDNYYTLEISPNI
                     RNSEEKQILETNIYNLIKKSEQLGGLLLKNNNIANPDKNNYIPENGNVFFINNNFLNF
                     YKNINHDFKMIDNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKKVDVQVLSK
                     NVSVFSFDRTTNLKFGYLDSPIVMLLTPDDLTGDFYLAAVSQGGYLFKDLDTLKQLLK
                     DNHLEEEISGITNYKDSVLNELNETKTKMIITIVTIIINVFILLIATVFETIQYFSWN
                     KKQLLLRKIHGYSLFSSNVRYLTISILLSIMLAYTTHILFGSKILLFIIMSIAIIQHL
                     LQIAYIKYLEKYFKDLMREI"
     gene            complement(10113..10406)
                     /locus_tag="P6L01_001022"
     CDS             complement(10113..10406)
                     /locus_tag="P6L01_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HDP3030105.1"
                     /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGK
                     HYVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH"
     gene            complement(10785..11180)
                     /locus_tag="P6L01_001023"
     CDS             complement(10785..11180)
                     /locus_tag="P6L01_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent arsenate reductase"
                     /protein_id="HDP3030106.1"
                     /translation="MDKKTIYFICTGNSCRSQMAEGWGKEILGEGWNVYSAGIETHGV
                     NPKAIEAMKEVDIDISNHTSDLIDNDILKQSDLVVTLCSDADNNCPILPPNVKKEHWG
                     FDDPAGKEWSEFQRVRDEIKLAIEKFKLR"
     gene            complement(11198..12490)
                     /gene="arsB"
                     /locus_tag="P6L01_001024"
     CDS             complement(11198..12490)
                     /gene="arsB"
                     /locus_tag="P6L01_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HDP3030107.1"
                     /translation="MMTILAIVIFLLTLTFVIWQPKGLDIGITALIGAVVAIITGVVS
                     LSDVLEVTGIVWNATLTFVAVILISLILDEIGFFEWSAIHMVKASNGNGLKMFVFIML
                     LGAIVAAFFANDGAALILTPIVLAMVRNLGFNQKVIFPFIIASGFIADTTSLPLIVSN
                     LVNIVSADYFDIGFIEYFSRMIIPNIFSLIASILVLWLYFRKSIPKTFDTENLSDPKS
                     AIKDSKLFKLSWIVLAVLLVGYLVSEFIQIPVSIIAGIIAFIFVILARKSKAVHTKQV
                     IKGAPWNIVVFSIGMYLVVFGLKNVGITTVLGDVLTNISNYGLFSSIMGMGFIAAFLS
                     SIMNNMPTVLIDAIAIGQSSATGILKEGMVYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKGVKISWGTYFKTGIIITIPVLFVTLLGLYLTLIIF"
     gene            complement(12487..12801)
                     /gene="arsR"
                     /locus_tag="P6L01_001025"
     CDS             complement(12487..12801)
                     /gene="arsR"
                     /locus_tag="P6L01_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HDP3030108.1"
                     /translation="MSYKELSTILKILSDPSRLEILDLLSCGELCACDLLEHFQFSQP
                     TLSHHMKSLVDNELVTTRKDGNKHWYQLNHAILDDIIQNLNIINTSNQRCVCKNVKSG
                     DC"
     gene            complement(12862..13620)
                     /locus_tag="P6L01_001026"
     CDS             complement(12862..13620)
                     /locus_tag="P6L01_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDP3030109.1"
                     /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSS
                     RYYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYTAPAEKQSGKSIENRPVFQEV
                     LNFLRMGDRLIVESIDRLGRNYEEIIQTVNYLKDKEVQLMITSLPMMNEVIGNPLLDK
                     FMKDLIIQILAMISEQERNESKRRQAQGIQVAKEKGIYKGRPVLYSPNAKDPQKRLVY
                     YRVVELLEQGKSISTIAKEVGITRQTIYRIKNSK"
     gene            complement(13613..15055)
                     /locus_tag="P6L01_001027"
                     /pseudo
     CDS             complement(13613..15055)
                     /locus_tag="P6L01_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(15027..15620)
                     /locus_tag="P6L01_001028"
     CDS             complement(15027..15620)
                     /locus_tag="P6L01_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDP3030110.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNG
                     TPIKTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN"
BASE COUNT         5277 a         2536 c         2050 g         5894 t
ORIGIN      
        1 tcaataaatt ttttcggcat aaatacgatg ttccttattt atcctcataa aaaacaatag
       61 ctgaaatata taaccaatta ctgtgtgttt gaatttctaa tacatgaata tcattcttta
      121 aaaacctatt aacttgttta tctaattgac gatgagtaaa agtagcgaaa gttttaatct
      181 tcatgatatc tctcctttta taaaaatagc aatccttatt acaatattaa acacttttat
      241 tttggatttt aagaggcatt ttgaagactt agaggcaaat catataggtt ttgtacttaa
      301 aaatctatac agtctcttaa aacgcaaata tgagccaaat aaatatatgc taatttcaca
      361 aacaaaaatt tcagcaaaat cagtgactaa ttttttagat actgcccatt tacatgcaaa
      421 tttaaaaatt agcataaaat actttaaaac ttccccatgt ttttctaaat tcctgcaggg
      481 cttttcttat caaaaactta gaaattttaa tttaactcta aaatctccgt aagagcataa
      541 gggaaatttg ctatcattca aaaatgatag atctggcaag gccagacatt ccaagggttt
      601 taagttattt taaagtgata tcatacaaag ctgtagtgtc acacttttgt gacactacat
      661 acagcatttg tcacaaaaat aagacagcac ttttttacag aaaaacaata aaaaaagaca
      721 tcagttatca ctaatgtctt aaataataat tttgcttttc gaaattccaa attgcggtaa
      781 ttgaatgtgc tttttttctt cgtctgtcgt tttatcttca tcacttgtat taatcaaatt
      841 accatcttcg gcatcgtcta agtttaaaat tttatgcttt tgtttaagca atccaccata
      901 acttaacata cgttttcgat acaaaccttt ttctaaatca cttacaattt cttgattttt
      961 ttcgtcgtca tcagttaaaa agtcagatga tttaacggaa tattttgatg tctcttcgag
     1021 tgttataaaa tcatttgaat tattgattaa agatattata ttgtttcatg tttttacggc
     1081 gcatataaag ggcatgtgtt tcttcaagtt cagttagaat tgtttgcatt aaataagctt
     1141 gcattgactt gattgtttcc ttttggcctt taactcccct atcatgtata gcttttttaa
     1201 gtctttttaa agaagttaat atttcatcct caatgtcctc taacatgtaa tgcatatctt
     1261 catgccttga attaaatgat ttttttgctt taagaataac agcctttatg atttctaaat
     1321 ctctataatc atattttaat aaataattag ttagttgttc tgggaattct tgcaatagca
     1381 tttctttatc atcaaaatta tgtgtaaatt gatttgaatg atttgaatga ttactaattt
     1441 catttttgta ctttatatca ttcaagtcat tcatatcatt attctcagta tcattaaagt
     1501 caatgtcatt aaagtcagta tcattagtgt tcgattttcg aacttctgga cgttcgattt
     1561 tcgaacttct ggacgttcga aaatcgaact tctggacgtt cgattttcga acttccttct
     1621 cgccgtaagg ttcaacatcg ttttcttctt tttctatttt gtaaatatca cgttctgtga
     1681 caataggttt cattaaatat agaatatttg gtttatttaa accacgtcgt ttttgaatga
     1741 gtagaccatc gttttctaac ccttttttta tttttgttaa cttttctttt ttacaattta
     1801 agatttccat tagcttttca tttgaataaa caaagtaaat gttaccatct tcatcaaccc
     1861 agctattttt aatagataaa tttaagcgat cacgtaagat agcataggct attttcatat
     1921 cattagttag atttttataa ttttcactgg taaagaatac tttagggatt tgataaaatc
     1981 tttctttgta attttcttgc actgtaaaat attgattttg catgacaaaa aacctctttc
     2041 catattttag tttggtaaga ggtaataaaa cgtttttatt tagctttaca tttatttaat
     2101 gatttaggtg cttgcactaa gatattattc attatataat acaagtatcg aaaattatat
     2161 aaataaacag tttctaaacg ttttattacc aaaccccgac tattaccagt agttggggtt
     2221 tttgttatat taagtttatt atagcacttt aaataaactt ataaaaggga aaatatagct
     2281 ttatcaaggg tttataaacc cttgataaat atcataaacc tttcataaac ccttgataaa
     2341 aaggttgatg gatgtttatt tttatttatt aatcacttta ttattaagca ttataagctt
     2401 ataatatttt atcaagggtt tataaacatt ttataaaggg ttgatgatat gaaaagtgtg
     2461 aaaaagttga gtgaagaatt aggggttagt aagcaaacga tttttaataa tattaagcga
     2521 ttgaatatag agacaataaa acaggaaaac acttcgttta ttaaagaaga taccgatata
     2581 gaaaaaatca ttcaaagagt aaatgaaaat aaaaagaaat atggttttga aagtactact
     2641 gaagacaaac aaaaaaaagg gtcggataat attaattttg aatcgaaaag cgatgctcaa
     2701 atagtagaaa tccttaaaaa tcagattgat actttaaata atcaaattga aaaacaagaa
     2761 agtcgtcatg aaacaacaat tgaattttac agaaaagagt tgcaagaaag atcaaaattg
     2821 cttgaaaatc agcaagttct ggcattagag agtaataaaa agattcaaaa attagaaaat
     2881 caattagaaa aagaaaaaca ccttaattat ccctttgata catctactaa cactagacaa
     2941 aacgttgatg cacaagaaaa aacatatact actaatcctg taaatattaa cagagatcag
     3001 gaagaaacta aagaaacaga gattcaatat aaggatatat ctggaagtca aagcgacgaa
     3061 agtacacaag gtgaagaagc aaaaaaagaa gacgttccag ctaatctaaa tgacaatgat
     3121 agtgatatag aagaaaagag tgaagaaacg gaacctaaaa aggggttttg gagtcgtttg
     3181 tttggtagct aaaataattg attaggcact aaaagttata aaattttagt gtcttttata
     3241 tagtgagaac ttctggtaat catcgattat gttaaccaaa caaaaagcca taaatgccta
     3301 tatgacggca tttatggctt tttactttca ttatctttta tgcatctttt atacatcgtt
     3361 gatttgacgc gttttcattc ttttgcattg gcaagtattt tgtatcttta ttcagttact
     3421 atttcttttc tccaacctaa caaaccatct ttaactctaa taatatgttt gccacgccag
     3481 gcacttctta cttcgaaata ataccattcc ccgtttttgt tgaggaaata aatatcttga
     3541 taaccttctt taatgacata ttttgtagct tcttgtcttt gatcgccaaa cattatcaat
     3601 cccaccctta tatttattta tctttaatat ataacaacat ttattaatat tcatgttatt
     3661 tcatggaaga agagatttgt tataatgtga aggtcgtcta tctctcagac gtcaattcgg
     3721 cgtagagagg aggtgcgatt tatgaatgaa attcttgttc acatcatgac aacggcaatc
     3781 agtggttgtc tcgttacgtt atttggttat tggctccata aacgtgataa aaaataatat
     3841 agacgattaa agccaattca ccaaaaaacc ccgaactacg gcgaatagtt cggggtttgg
     3901 tgcgtttaca aacgaaattc ttgttacttt tagtatatat ttcatattgc cttttgtaaa
     3961 ctcttctatt cttatcaata catttcaagg tgtcgtaaaa atactttcct ttttcatatt
     4021 gcctgatgtg cattaaattt tgattttaaa atcaacccat gaacgaatga atggcccaaa
     4081 ctaaaaatcc ggcaacagga aagaaaagcc accaaaaatc ataaagaaaa tcaactatgg
     4141 aaggtgccct gttactctct ctataatttc cctctaaaat aactttttta aaacgtgcat
     4201 ctcctttaat taaatgctct aaagagattc caaataaatc gctcatagca atcaaaatat
     4261 ctaaggaagg atagtttttg gcactttccc atttagaaac actttgtctg gaaaccttta
     4321 atatattggc taattcctct tgagaccatc tttttaattc tctctgttct tttatagctt
     4381 cagctaattt cataaaatca ctccttaact caaactataa ttaaaataat tgaaaagtca
     4441 cgatactttt agttaaccaa ctcgcaacga aaagttgcgt tattcccaat tcagttcaag
     4501 ttgttttaaa aaatcctctc gttctttctc taattgtggg tcgtaatctt tttgaggttg
     4561 cttttcatat gaccgttctt ctaaccattt aggcgtcttt tcacgcctca catattgtct
     4621 tgatttatct atcttggaca tttgaggttg tcgtttgtta tgaatgcgtt tctcagcgtc
     4681 aaatgtgaac tctatccatt caattttacg cccttttttc gctttgattt tattgatagt
     4741 aagattatta aatatagaac ctaattcaat aataataggt ttaaaaacat ttttgttaat
     4801 atcagtcata cgataagatt taggtatatc taaacgattt ttaaaatcat cgatgtgaat
     4861 ttttaaatag cctgtatgtt tgtattgttt aagtaatcta aacatatttt tagaatatgt
     4921 agatttaagc ctagtcattt cttttaattc aaattttgta aaattattag taattgaatt
     4981 caatatgtgt tttaaatttg aagaagttga tatttctaga tattgttctc gcttatggat
     5041 tctataatga ttgaataaaa cgaatatttc tattacatca tcatcttcat atctaatggt
     5101 taaattcaac atctttttat acacattatc taaatcatta gcaaatctat taatattacg
     5161 tgtttctgga ctataattac ttaatttttt taattcatca aaagacaaac gtaatgtatt
     5221 agtatcttgc tcttttaatt tattacacat cgcaaaaaat aaattaattt cagtagctgt
     5281 aaatcgcctt aatggaacta aattcatttc atttttatat acaactgtct ctcctgtcat
     5341 gatacagccc ccctttatta cgaacctaaa ggagtatcgt ttgaatatac actcccattt
     5401 gaaaactttt attcacaata aactacttta ttatcaatgt caaaccataa aaggagtaca
     5461 actttttaaa acaccccttt tatgtggcaa aaacactcct ttaggcattg aaatcccttg
     5521 ttgctctagg tctgaacaag cccctaaaag atttttaaaa agatttataa aaatatatta
     5581 aaaagttaga ggggggctac ctatttttca tcctaaaaaa taattaacct caaacaaatg
     5641 catttaaata tctattttta taatgtataa ccatcttctc tatcttcttt aaaaatgatt
     5701 tctcattctt gtgcttgata tttcttctta aagaactgtt catcattgtt atctatcgtc
     5761 attacttcac gtattttaga attgtctaaa tgattatcaa tatgatttat tagcgtatga
     5821 taacgctctt ctttcatcat acttttaatg attttaaaaa cttgattgaa taacttaagc
     5881 gtataattta gttctttatc tttcttttca ttctctgaca acaaataatt ataacgttgt
     5941 gctaaatcat taaattcttc aaccagcttt tcattttcca tatggacact agtcaattca
     6001 ttaactaaac tttcattttg tttctctgat ttttttaatg acttcccact tttaatccat
     6061 tcataatcat ctaatatcat ctgtgctgct ttaatttgtt tagtgtattt ttcaaaataa
     6121 gttttagtaa ttacaatatt gcctgtttct tgcttctctt tatgaaacca tccacctaca
     6181 tttttagttt taatttcata catgacactc agaagctttt taagcgtatc taatgatttt
     6241 tggtagtttt gtatcaattt atcattttct tactttaaat gatcctctat ttgcttctcg
     6301 cgcatataca tttgtttatg atattctgtt ttttgtttat agccgctaac ttgatcatgt
     6361 ttttcttttg taagttgtct agaaattcct cgttttaaat catagccttg cttattaata
     6421 tacgtattga atctatcttg gaattcggtt aatgcttttt tattacctac aacttctttc
     6481 gcacttaaac gtccatcttt agtaatagga attacgccat aatgcatatg tggtgttatc
     6541 tcatccatat gtactgtcgc atataaaaga ttttctttac cgtattcttc ttccaagaac
     6601 gctttagcat gcttaaaaaa ctattttgtc tcttcttcag ataattgatt aaaaacacat
     6661 tatctgatgt aattaatcca tcaatatgtt taatggaatc tgttctaatt ttgcgtttcc
     6721 ctttataatt ttgttcgatt ttctcatcaa tttttttatt aaaatcaaac ttagtatcat
     6781 tgactaaatc atagtttaaa tatgacttac ttaaatcaat atctatattt tcataatttt
     6841 tattttctct ttgaatacct cttgtatttg tttttgattt cactttaaca actcgaataa
     6901 ttgaataaga cataataaat caacttccac tgcatatgat tataacacct aaacacagaa
     6961 aagttttaac atactagact tattaaaaac caaataagtc attaaaaacg tgtgctctgc
     7021 gaggcttgtt ttttgtcatc acaaaaaagg actggaaaat ccagtcctta aattaaaaaa
     7081 ctcaaaagta atgactaatc atatttcata ttgttttttc tctatgtatg gctataccta
     7141 tcaatatttc ttaacttcta agctcagaaa tattgactat gacatcaaat cgtttcgcaa
     7201 gctctaaatc atgcgttacg acaatcatag ttttattttc atcaagtagc ttcaataata
     7261 aatcaataac tatttttcca tttttcgtat ctagtgatcc agtaggttca tcagctaaaa
     7321 tgattttcgg atcttttaat attaatcttg ccaatgctac tctttgttgt tcaccaccgc
     7381 ttagagaact aacaattctc ttgatatcta aatctaggcc gacctttttt aatacttctt
     7441 tctttaagtt catattatct tttttattct ttttgaactt tagggctaaa tctaaatttt
     7501 gactgattgt ttgattttca agtaaagcaa aattttgaaa caaataccct aacgtatatc
     7561 tataaaaatc gactttcttt tttagtaact tatcttcaat atatatgctt ccttgtgata
     7621 catcttcgaa cccagcgatt atgttaagta aagtagattt tccagcgcca ctttctccaa
     7681 ctaaagcata tgattttcct ttttcaaaat taaagttaca tccgtctaaa atgtttttat
     7741 tacctttttt tacaaaaaca ttttcaagtt ttatcactat cggtcacctc attttaaaaa
     7801 gctaggaata tcttttgtaa tgtattctat cgaacgcaca tatttccctt tatgatttac
     7861 tttcacatat tcttttgtag gatcattacc taaaaaatcc aatttataac tctttttttc
     7921 tccattttta gagtatacag ttatatttct atagtcctgg gtatcttttg gaactttggc
     7981 ataagatact ttttctttta acagtgggtt aaaacgatct aaatgctcat ttctaaccaa
     8041 taacagacta agtatgatga ttaatagtac aattgttaca ataattaaag ttcttttcat
     8101 tgtttaaatc tccctcatta aatctttaaa gtatttttca agatatttaa tataagcaat
     8161 ttgaagtaaa tgttgaataa tagcaataga cattattatg aatagtaata tctttgaacc
     8221 aaataaaata tgcgtagtgt aagctaacat aatagaaagt aaaatagaaa ttgttaaata
     8281 acgtacatta ctgctgaata atgaataacc atgaattttt cttaataata gttgcttttt
     8341 attccagcta aaatattgaa ttgtttcaaa tacagtagct atcaaaagaa tgaaaacgtt
     8401 aataataata gtaactattg ttattatcat ttttgttttc gtttcattta attcatttaa
     8461 cacagaatcc ttgtaattag ttataccgct aatttcttct tctaggtgat tatctttaag
     8521 caactgttta agtgtatcta aatctttaaa tagatatcca ccttgagaaa cagcagctaa
     8581 ataaaaatct cctgttaaat cgtcaggtgt caacaacatt actattggag aatctagata
     8641 tccaaatttc aaattagttg tgcgatcaaa agaaaaaaca gatacgtttt ttgacagtac
     8701 ttgcacatct acttttttgt tttgttgctt ttgaaagtca atccatcctt ggtgattttt
     8761 ttgaatttca gatttttgat attgtaacct tggaggaata aacacgttta ttttatccga
     8821 atgattgtca atcattttaa aatcatgatt tatattttta tagaaattca gaaaattgtt
     8881 attgataaaa aagacattgc cattttcagg aatgtaattg tttttatcgg gattggctat
     8941 attattattc tttaaaagca atccaccaag ttgttcgctt ttcttaatta aattgtatat
     9001 attagtttct aaaatttgtt tttcttctga atttctaata ttaggagaaa tctctaatgt
     9061 atagtaatta tctaactttt gccagatttt ttcagagtca tatatattct ttaacttcgt
     9121 ataagcattt aaattttgtg ccagtaaaaa agatagtatt agcaccatga tacatttggt
     9181 gaaggtaccc ataaatattg aaaacttata tccttttttc ccttttaagg ttaatggaat
     9241 acttaacttc aaaagaagac tatacgaaat cagccacaac aatgtagtaa tacaagcaaa
     9301 aacaaaaagc aacacgataa atctacgaat aaacattaag aaatccaatt ctaaattggc
     9361 tatatgaatg attaatatat tagcaataaa gaaaatagtg gtaagtacta gccaataaat
     9421 gatttttata tgaatattat gaaaaataat gcgtgtagtt gaaaagccat gtagcttttg
     9481 aatagcataa aatttcaaat tatatcctct gtcatataag aagtacaaaa gatataatac
     9541 gcctaagaat atgaaaggca ctaataaatc attatctatg actaattcaa aaaataatat
     9601 gtttctattt atgtattcaa cttcagatgt taaaccagct ttttttaatt cactcgtaag
     9661 ctcttctaca ttgaaatgtt caccaataat ataatacttt ccttttatct ctctgtttaa
     9721 taaatctgat ggagttaaaa gtgatgtttt attacgtata aagtcagaat tatttcgttt
     9781 tgagttgaaa acaaaaaatt cttttttatt atcaaaatct ttaacggatt taattatttg
     9841 tatattttga tttttggcgc ctgattcaat gacatctaac acttttgatt tactgttaga
     9901 tttatcccaa ttagagattg ttaaaatatt ggaactttca ggtataattt cttcattttc
     9961 tttgaagaaa aataaaaata atagccctga aattaataat aaagtactaa aatctaaaaa
    10021 taatttgaat tttttcatat tgcctctttc aagagaatag acagaattaa gtttacttaa
    10081 ttctgtctat tctccttaat taatatatgt aattagtgaa gactataaaa tgttttgttt
    10141 ccaccccaag cctttggagc agatgcctta gattgaacac caggtcgagc aacaccacta
    10201 aaagatgaat atttccctac tgctgttgaa ccatgatttc ttttattgtg actataataa
    10261 gaccatacgt aatgttttcc aataccatgg ctccaaacac caccagctac atgtactgtt
    10321 gcagcttcag ctttataagt tacacctaat aaagtgccga ataagattgt actagcaata
    10381 caactagaaa caaatttctt tttcataaaa acaactccat ttaatattct ttatttatta
    10441 tagcaaaaat aaataaaata gagaatgagt aaaaataatc taaaagtgtt ttttgctctg
    10501 ttgcttgttt tgttcatcta tatataatat taattctatt atccttaaat taactatcat
    10561 taaacaacga tatgacaatg tataataaat acaatttcac tacctataat tcaacattat
    10621 gtaaactaaa gtatttcaaa taattaactg tattaatact ctcatcatga ttgcgttata
    10681 aacgttcgtt aatgagaaaa agagagggca tattgtatat ggcacaaatt ttgttcttta
    10741 aatggcaaaa cacctcttat taactaaata agaggtgttt atgattatct caatttaaac
    10801 ttttctatag ctaatttaat ctcgtctcta acacgttgga attctgacca ttctttacct
    10861 gctggatcat caaaacccca atgctctttt ttaacgtttg gtggtaaaat aggacaatta
    10921 ttgtctgcat cactacataa cgttacgacc aaatctgatt gttttaaaat atcattatca
    10981 atcaagtctg acgtatggtt tgatatatca atatctactt ctttcatagc ttctattgct
    11041 ttaggattaa caccatgtgt ttcaatacca gcagaataga cattccaacc ttcacccaat
    11101 atttcctttc cccaaccttc agccatttgg ctacgacaag agtttcctgt acatataaaa
    11161 taaattgttt tcttatccat aattaaagcc ccttttctta aaatatgatt agtgtaaggt
    11221 ataaacctaa gagtgttaca aataggactg gaatagtgat gataattcca gttttaaagt
    11281 atgttcccca cgaaatcttc acaccttttt gtgttaagac atgtagccac aataatgttg
    11341 ctaaagaacc aattggcgta attttaggtc ctaaatcaga acctatgaca ttcgcataaa
    11401 ccatgccttc ttttaatatt cctgtagcac tggattggcc aatcgctatt gcatctatta
    11461 aaacagttgg catgttgttc ataatcgaag ataagaaagc agctataaag cccataccca
    11521 tgatgctgct aaataaccca taatttgaaa tgtttgttaa aacatcccca aggaccgttg
    11581 taatacctac attttttagt ccaaacacaa caagatacat accaatagag aatacaacga
    11641 tattccatgg tgcaccttta atcacttgtt ttgtatgaac tgctttagat ttacgagcta
    11701 atattacaaa gataaaagca ataataccag caataattga tacaggaatt tggataaact
    11761 cgctaacaag atatccaacg agtagtactg ctaatactat ccatgaaagc ttaaataact
    11821 tagaatcttt aattgcactt ttaggatctg atagattttc tgtatcgaac gttttaggta
    11881 tggattttct gaaatataac cataaaacga gaatactagc aatcagagag aatatattag
    11941 gaatgatcat tcggctaaaa tattcaataa atccaatatc gaagtaatct gcagaaacga
    12001 tattaactaa gttacttaca attaagggaa gtgatgtagt atcagcaata aaaccactgg
    12061 caataataaa ggggaaaatc actttttgat taaatcctag attccttacc atcgctaata
    12121 caataggcgt taagattaaa gctgcaccat catttgcgaa aaatgctgct acaattgccc
    12181 caagtaacat aataaaaaca aacattttta agccattacc gtttgaagcc ttgaccatat
    12241 gtatcgcaga ccattcaaaa aaaccaattt catctaatat taatgaaata agaataacag
    12301 ccacaaaagt taaagttgca ttccaaacaa tacctgttac ttctaataca tcagaaagac
    12361 ttacgactcc tgtaatgata gcaacaaccg ctccaattaa agctgtaata ccaatatcta
    12421 aaccttttgg ttgccaaatc acaaaggtta aagttaaaag aaaaattaca attgctaaaa
    12481 tagtcatcaa cagtcacctg atttcacatt tttacataca catctttgat tagatgtatt
    12541 aatgatgttc aagttttgga taatatcatc taaaatagca tgattaagtt gataccaatg
    12601 tttattgcca tcttttcgtg ttgtaactaa ttcattatct actaatgact tcatatgatg
    12661 acttagtgta ggttgtgaga attgaaagtg ttctaataag tcacaagcgc atagctcacc
    12721 acaagaaagt aaatctaata tttctaacct acttggatct gataaaattt ttaatattgt
    12781 tgatagttct ttataagaca tgaataaacc tcctagatat taaatagatt aacatctata
    12841 tagattattg tctatgttac tttatttact attttttatt ctatatatag tttgacgtgt
    12901 aataccaact tctttagcta tagtacttat agatttaccc tgttcaagta attcaacaac
    12961 tcggtaataa actaaacgct tttgtgggtc tttagcattg ggagaataca gaacaggtct
    13021 ccctttatat ataccttttt ctttggcgac ttgaatccct tgagcttgtc gacgtttact
    13081 ttcatttctt tcttgttctg aaatcattgc taaaatttga ataattaaat ctttcataaa
    13141 tttatctagt agtggatttc caatgacttc attcatcata ggtaaactcg ttatcatgag
    13201 ctgaacttct ttatctttta agtaattaac tgtttgaatt atttcctcgt aatttcttcc
    13261 tagtctatca atagattcaa caattaaacg atctcccatt cttaaaaaat ttaatacttc
    13321 ttgaaaaaca ggtctgtttt ctatagattt acctgattgt ttttctgctg gtgcagtata
    13381 aaatatttta tccaccgata gtatgtcatc ggtcgtttta ctagctaaat cttcccaacc
    13441 tctatggtta acttgttttt caatagtatc ccaatttgtg taatacctag acgataaagt
    13501 ttttccgaca ccaggttgac cataacatat accaatatac tgatatttaa tacaagcatc
    13561 acaaaattca gcaaagcgtt tatactcttt cgtttcaatg aaattttgat ttttagtcat
    13621 tacgatacct ctttaatttt ttgacgtttt tagtcgtttc ttgaggtgaa taaccacaat
    13681 ttttttcttc cttaattaat tccgtggtac ttggactagc aatattttgt tttaagtgtt
    13741 ttcttctctg actacgagcg tgctgtatgt cttttatatc tatggagtaa tcagctattt
    13801 ctggacatat agcagtacaa agaaattaat ctctataaaa cacacgtatt tcagccatat
    13861 catttgggtt ataacgaatc agtacatatt cacctacata agctgctaga ttagtattac
    13921 tgtatcgaaa accttgaaaa tgaatgccat ctgaatgaac ttttctagat ttaggtattt
    13981 ccaacaataa taaatcaagt tgttctaagc tactcggcat attagggaaa aagtgattac
    14041 tattccaacg actgataggc gtggtatgaa tagtactatg ttctttttga ttataatctt
    14101 caattaaaaa gtaacgtaat ttttcttcaa aattgtgaaa atttattaga ttactaggag
    14161 tatcattatt gtttatatat ccaggcagtt gttctaagaa agtttgatta actgtctgaa
    14221 agaatcgttc tatttttcca cgaccacgag gaacaccaac tttcgaaaac atcaagttaa
    14281 ttttcaaatc aatagcgact tgttccatat gatgtgaagt aaagtcactt ccatgatctg
    14341 tgtagaattt ttcgggtata ccacacactg gccaattggt atcattttta ttccatattg
    14401 cttggtgtag tgttaatgct gtgttttgag cattgggggc atcaaaactt aagaaatagc
    14461 ctgcaattgc acgactatag tcatccataa taatagttag ccatggtcta ttgatattac
    14521 ctttttggtc taatataaag atatctagca aagtatggtc tgcttgccat atttcattgg
    14581 gtctcgagga ttctctaatt tgtattaaat cgtacttatt ttgatagtat ttttcaccct
    14641 gatgagagaa atcaataaca gacttaggca tcgctttaat aacactataa atttgtttat
    14701 aacttggtat atcaaaatta ttttctttac agtaattagt ggttttccta tgaattgtgg
    14761 ctatactatt tcttttattc ataagcgcaa ggcgttcaat ttcatcaatt atctttggtt
    14821 ctattttacg tgttccttta tcagcttttg tcgcatagat tagtccttta agtccttgtt
    14881 ctttatatga cttattccat ctataaaggg tgcttaacgc aatcccttta cttttagaaa
    14941 tacttgataa agattgttcg cctaggataa aaggttttat aatattgtac ttttgagtag
    15001 cttttttacg tttggcttca gaaaaattag ttagatactt ctccttgttt ttcatcttcc
    15061 ttgtcctcca atttattgag gtaacgataa attgttgtac gtgaaacatg ccactgttct
    15121 gcaattgtct taattggcgt gccattatca taaagtgtct taagtaagtt taaatctttt
    15181 tgatttaatt tttctggtct gcctccatat cggcctctag cacgtgctgc aattcggcca
    15241 gctgaagaac gctctaaaat taaattacgt tcaaattctg caaatgctgc gaataaatga
    15301 aataataatt gtccagttga acttgattta tccattgtta tattttcttc taaactatgg
    15361 aaacttacac ctcgttcgtt aagctcatta actaatgtga ttaaatcctc catattacgt
    15421 cccaatctat ctaatctcca aacaacaatc gtatctcctg acctcgcaaa ttcaattgct
    15481 ttatctaaac caggtctttt actttttgaa ccactcatat ggtcattaaa tatcttttca
    15541 caaccatatg tatttaatcg atcctcctgt aaatttaaat tttgtaatcc agttgaaact
    15601 cttgcataac ctattttcaa aatacaaacc tcctatatac atcttcagtg taccataact
    15661 taattacaag gccttttatg aacatagatt ttttgttggg tttttgaaca cataatccta
    15721 tgtaattctt acttatttag acataaaaga agagtgt
//
LOCUS       DAOBBH010000043        15668 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.043, whole genome shotgun sequence.
ACCESSION   DAOBBH010000043 DAOBBH010000000
VERSION     DAOBBH010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15668
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.043"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..103
                     /locus_tag="P6L01_001029"
     CDS             <1..103
                     /locus_tag="P6L01_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDP3030111.1"
                     /translation="LLKYKIDNYDNHIAKINASDNHDDNVERLWERK"
     gene            173..1021
                     /locus_tag="P6L01_001030"
     CDS             173..1021
                     /locus_tag="P6L01_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDP3030112.1"
                     /translation="MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDT
                     AQSYLNETEVGQGIEASGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYLDL
                     VLIHQPYNDVYGSWRALEELKENGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
                     PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRWLV
                     ERDIVVLAKSVNPERMAQNLDIFDFELTEEDKQQIATLEESNSQFFSHADPEMIKALT
                     SRELDV"
     gene            1254..1457
                     /locus_tag="P6L01_001031"
     CDS             1254..1457
                     /locus_tag="P6L01_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030113.1"
                     /translation="MKYKHFAIITLITGIVMLTAMYFVPFGYHKIPIFAGMAIIFTVY
                     FSNKKSIDETNIFSKKNRNKNKQ"
     gene            complement(1544..1705)
                     /locus_tag="P6L01_001032"
     CDS             complement(1544..1705)
                     /locus_tag="P6L01_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030114.1"
                     /translation="MLLILELRFWNLEFGFRNSTFGIQNYNEWKILIIACLMTWFCSD
                     FYLMNCFFV"
     gene            complement(1742..2482)
                     /locus_tag="P6L01_001033"
     CDS             complement(1742..2482)
                     /locus_tag="P6L01_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDP3030115.1"
                     /translation="MFILRNDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGL
                     FDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVDTKPNIVHDWIAKLERNQQS
                     LGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNACHKSYTKSDVIDRTLKHCDNCGGA
                     IRPDIVLYGEMLDQPTIIRALNKIEHADTLVVLGSSLVVQPAAGLISHFKGDNLIIIN
                     KDRTPYDSDATLVIHDDMVSVVKSLMTE"
     gene            2688..3479
                     /locus_tag="P6L01_001034"
     CDS             2688..3479
                     /locus_tag="P6L01_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDP3030116.1"
                     /translation="MTHRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVS
                     DMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRYIARKAASL
                     IEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMR
                     HITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYL
                     LIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD"
     gene            3846..4274
                     /gene="lacA"
                     /locus_tag="P6L01_001035"
     CDS             3846..4274
                     /gene="lacA"
                     /locus_tag="P6L01_001035"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HDP3030117.1"
                     /translation="MAIIIGSDEAGKRLKEVIKSYLLDNKYDVVDVTEGQEVDFVDAT
                     LAVAKDVQSQEGNLGIVIDAFGAGSFMVATKIKGMIAAEVSDERSGYMTRGHNNSRMI
                     TMGSEIVGDTLAKNVVKGFVEGKYDGGRHQIRVDMLNKMC"
     gene            4290..4805
                     /gene="lacB"
                     /locus_tag="P6L01_001036"
     CDS             4290..4805
                     /gene="lacB"
                     /locus_tag="P6L01_001036"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HDP3030118.1"
                     /translation="MKIALGCDHIVTDTKMRVSEFLKSKGHEVIDVGTYDFTRTHYPI
                     FGKKVGEQVVSGNADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGRIIGELLMCDIIDAFINAEYKATEENKKLIAKIKHLETSNADQADPHFFDEF
                     LEKWDRGEYHD"
     gene            4818..5750
                     /gene="lacC"
                     /locus_tag="P6L01_001037"
     CDS             4818..5750
                     /gene="lacC"
                     /locus_tag="P6L01_001037"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HDP3030119.1"
                     /translation="MILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTR
                     VLAQVGEPVLASGFIGGELGQFIAKKLDHADIKHAFYNIKGETRNCIAILHEGQQTEI
                     LEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPV
                     ILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEW
                     IIVSLGAQGAFAKHNHTFYRVNIPTINVLNPVGSGDSTVAGITSAILNHENDHDLLKK
                     ANTLGMLNAQEAQTGYVNLNNYDELFNQIEVLEV"
     gene            5754..6734
                     /gene="lacD"
                     /locus_tag="P6L01_001038"
     CDS             5754..6734
                     /gene="lacD"
                     /locus_tag="P6L01_001038"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDP3030120.1"
                     /translation="MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEP
                     TVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKG
                     RLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPF
                     FLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGF
                     AEGEVVYTKEEAAQHFKDQDAATHLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLC
                     GRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK"
     gene            6756..6986
                     /locus_tag="P6L01_001039"
                     /pseudo
     CDS             6756..6986
                     /locus_tag="P6L01_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500918.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
     gene            6981..8693
                     /locus_tag="P6L01_001040"
     CDS             6981..8693
                     /locus_tag="P6L01_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDP3030121.1"
                     /translation="MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSI
                     FLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTN
                     QINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
                     NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLF
                     TAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKI
                     ITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAP
                     LVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWS
                     FVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILA
                     ASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAH
                     MDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQ
                     QFEN"
     gene            8711..10123
                     /gene="lacG"
                     /locus_tag="P6L01_001041"
     CDS             8711..10123
                     /gene="lacG"
                     /locus_tag="P6L01_001041"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDP3030122.1"
                     /translation="MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWY
                     TAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNE
                     IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYQGEIGVVHAL
                     PTKYPFDPSNPEDVRAAELEDIIHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNI
                     TDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDKGGSKYQLKGVGQR
                     EFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVH
                     DDARIDYVRQHLNVIADAIKDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQE
                     RYPKKSAYWYKELAETKEIK"
     gene            10389..11258
                     /locus_tag="P6L01_001042"
     CDS             10389..11258
                     /locus_tag="P6L01_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDP3030123.1"
                     /translation="MKKIFVIITTLLAVAIIIGSIITVVFSQRQAQTFKIQQQQFVKK
                     PIPTLFLHGFGGSANSEKFMVKQAEKRGVTKDIITAYVSKDGAVTFKGKLRKDAVNPI
                     VKIELENNRQGYLDKNAAWFKNVLTKLQSEYNFDKFNFVGHSMGNLTFAQYMMTYGND
                     KSLPQLNKQVNIAGTFNGVLNMNEDVNEITVDKDGKPSRMNQPYQQLRVLKDIYKGKG
                     IEVLNIYGDLKDGTHSDGRVSNSSSKSLKYLLGNSPKSYRESKYEGEPAQHSQLHENE
                     NVANELIDFLWKK"
     gene            11536..12537
                     /locus_tag="P6L01_001043"
     CDS             11536..12537
                     /locus_tag="P6L01_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDP3030124.1"
                     /translation="MQNKQILFNKIPEGMPQEDTFKIEEIDTPKLESDGVLVQTLYIS
                     VDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINH
                     VQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGKPKAGDTVVVSAASGAVGSV
                     VGQIAKIKGCRVVGIAGGDKKVNYLTETLGFDAGIDYKKEDFAEALAQAVPDGIDVYF
                     ENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVS
                     EFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPHAFKNLFTGDNFGKQVIKVTE"
     gene            12772..13779
                     /locus_tag="P6L01_001044"
     CDS             12772..13779
                     /locus_tag="P6L01_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDP3030125.1"
                     /translation="MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNP
                     VDTKQRQMKVTQAPRVLGFDAIGTVEAIGPDVTLFSPGDVVFYAGSPNRQGSNATYQL
                     VSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISHNPSENVGKSVLIINGAGG
                     VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEKEIPLVDY
                     IFCTYNTDLYYNTMIELIKPLGHITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHR
                     TPDMIKQHEYLEDITKNIELGHYQPTTTQVFEGLSPENLYQAHQLLEKQSMIGKLVIN
                     I"
     gene            14087..15649
                     /locus_tag="P6L01_001045"
     CDS             14087..15649
                     /locus_tag="P6L01_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDP3030126.1"
                     /translation="MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIAL
                     HFSWYYLLLVLVILFVCVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLV
                     FWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
                     KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFL
                     FNVPSNFTMQVILIVIATILFTWSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTL
                     YILNTFTNGLGNYIANFFSMSLRIPSGGQKFQWLQNWTIFYWAWWISWAPFVGIFIAR
                     VSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQ
                     HYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSRGDINPKSFVKVSWGIIMALFA
                     IIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLLKSLSEEKPRNSNKVLIKHRDPDI
                     LEYRSSKHIDEN"
BASE COUNT         5529 a         2305 c         2991 g         4843 t
ORIGIN      
        1 tttactaaaa tataaaattg ataattatga taatcatatt gctaaaatca atgctagtga
       61 taatcatgat gacaatgttg aacgtctttg ggagagaaag taaacctctg gcttgctata
      121 gagttcgctc catggtttat agtggacata caagataaag gaggttcatt ttatgaatca
      181 tattgaaata agtaaagatg taaagatacc agtattaggt ttcggagtat ttcaaattcc
      241 tcaagaacaa acagcagaag cggttaaaga agcaattaaa gcgggttata gacatattga
      301 tacagcacaa agttatttaa atgaaactga agtaggtcaa ggtattgaag cttcaggtat
      361 tgatagaagt gaactattca ttacgactaa agtatggatt gaaaatgtta actatgaaga
      421 tactatcaaa tcaatcgaac gatcattaca acgattaaat ttggactatc ttgatctagt
      481 gcttattcac caaccttaca atgacgtata tggctcatgg cgtgcattag aagagttaaa
      541 agaaaatggt aaaattaaag caatcggtgt atctaacttc ggcgttgatc gaattgtaga
      601 tttaggtatt cacaatcaaa ttcaaccaca agtgaaccaa atagaaatta atccattcca
      661 tcaacaagaa gaacaagttg cagcacttca acaagaaaat gttgtggtag aagcttgggc
      721 accatttgca gaaggtaaaa atcaattatt ccaaaatcaa ttgttgcagg ccattgcaga
      781 taaatacaac aaatcaattg cacaagttat tttacgctgg ttagtagaac gtgacattgt
      841 tgtactcgca aaatcagtga atccagaacg tatggctcaa aacttagata tatttgattt
      901 tgaacttaca gaagaagata aacaacaaat tgcgacatta gaagaaagta atagccaatt
      961 tttctcacat gctgatccag agatgattaa agcacttaca agcagagaat tagatgttta
     1021 atcataatat aacaaagtaa ataatttaca ttacgctaac ctgaaacttg gatgttaaag
     1081 tctatgttgc aggttatttt attactctta aaaagtgaat ggagcaagca aataagttat
     1141 tatgaatttt tgtattatgc aataacttac ctaagataat aattatagta ttgataattt
     1201 tataaaatga agtatataat gaatgtagaa taaattgtat aggggataat aacatgaaat
     1261 ataaacattt tgccattatt actttaatta caggcatcgt catgctcacg gcaatgtact
     1321 ttgtaccatt tggatatcat aaaataccaa tatttgcagg aatggcgatt atttttacag
     1381 tctattttag taataaaaaa tctattgatg aaacaaatat ctttagtaag aaaaatcgca
     1441 ataaaaataa acaataaaat gagttgtaag cataagattt acagttaatt ttttacgaat
     1501 gggcctttag cgattgaaaa tgttataaag tagcatccca acattaaaca aaaaaacaat
     1561 tcatcaaata aaaatcgcta caaaaccaag tcattaaaca cgcaataatt aaaattttcc
     1621 actcattata attctgaatt ccaaatgtcg aattccgaaa accaaactcc aaattccaaa
     1681 aacgcaactc caaaattaaa agcatctccc tgttactcgg gaaatgcttt ttacctactg
     1741 attactctgt cattaatgat tttacaacgg aaaccatgtc gtcatgtatg accaaagtag
     1801 cgtcgctatc ataaggtgtt cgatctttat tgataataat taaattgtcg cctttaaaat
     1861 gtgatattaa tcctgcggca ggttgtacaa cgagtgatga acctagtaca acaagggtgt
     1921 cagcatgttc aattttattt aatgccctta tgatggtagg ttgatctaac atttcaccgt
     1981 ataatacgat gtccggtcga atggcaccac cacaattatc acaatgtttc aaagttctat
     2041 caataacatc tgacttcgta taagatttat gacatgcatt acaataaaaa cgatttaacg
     2101 tgccatgtaa ttcatcaaca tgttgacttc cagcgtctga gtgcaaacca tcgatatttt
     2161 gcgtgatgac acctaaagat tgttgattac gttctaattt tgcaatccaa tcatgaacga
     2221 tattaggctt cgtatcgaca aatagtaaac gcttatggca gaaattgata aaaccttcag
     2281 gatcatcttc taaataatca cggcttaaca agtattctgg cgaaagtcca tcttttgaaa
     2341 tttcatcaaa taagccaccc attgaacgga aatctggaac gccacttgcg acagatacac
     2401 cagcacctgt gaaaaatgta atacgattcg aactatctat aatatgtttt aatgtctcta
     2461 aatcatttct caaaataaac accccatcaa aataatgaat gatttataac gtcttttgct
     2521 tttattatag tatattcatt ttacgattat caaaagcgca gtttaaacag ttctttttaa
     2581 aaatgaatta tacatataat cgaacagtta aattatatta gtttttacgc aatttttatt
     2641 atagaataag catgtatatt aaaattgatg tctaaacata aaggataatg acccatcgcc
     2701 taaataaagg aggcaatttt acattgaata aacatgaacg tttggacgaa attgctaaac
     2761 tagtgaataa aaagggcacg ataagaacga atgaaatcgt cgaaggttta aatgtgtctg
     2821 atatgacagt tcgaagagat ttaattgagt tggaaaataa gggcatttta acgaagattc
     2881 atggtggtgc acgcagtaat tcaacatttc agtataaaga aatatcacat aaagaaaaac
     2941 atactcgtca aattgctgaa aaaagatata tagctaggaa agctgcatca ttaattgaag
     3001 atggggatac tttgtttttc ggaccgggaa caacagtgga actattagca gaagaagtca
     3061 atcatcatac gctcacaatt attacgaatt gtttgccggt gtataaaatt ttgttagaaa
     3121 aacaaacagc acattttcgt gtctatttaa ttggtggtga aatgcgccat attacagaag
     3181 catttgtagg tgaaatggcg aatgccatgt tggaaaaact aagatttagc aagatgttct
     3241 ttagtagtaa cgcagtaaat aaaggtgctg tgatgacatc tacattagat gaagcctata
     3301 cgcaacaatt ggcactaagt aattcaattg aaaaatactt gttaatcgat catacgaaag
     3361 ttggcaaaga agattttaca tcattttgtc agttaaacga attgactgca gtggtcatgg
     3421 actatgaaga tgaagaaaaa gtagaaacga ttaaaacata tattgaagta gtcgactaaa
     3481 ttaaaagtat aactgcattg ttaaaatatg tatctatatg ctcataagtg aatgtgaatt
     3541 tgcattcatt tatgggttat ttttttgcaa atttatgtaa acgggaacat cgacaaagtt
     3601 ttactacaat ttcagacgaa tacctaatta aaacaacacc acaataggaa tgttcactta
     3661 ttgtttgaaa atatgtcata aacaaacaga ttataaacat attatgttga tttattgttt
     3721 gtttatgttt ataatttaaa cgtaatcaaa ttgaaagcct tttctcaaga ttttcataag
     3781 ctacaaataa atatgtcata ttttaagaaa ggtatttcaa aattaaagta aaaaggagtc
     3841 ttattatggc gattattatt ggttcagatg aagctggcaa acgattaaaa gaagtcatca
     3901 aatcatactt attagacaat aaatatgatg ttgttgacgt aacagaagga caggaagttg
     3961 actttgttga tgcaactttg gctgtagcaa aagatgttca aagtcaagaa ggtaacttag
     4021 gtattgttat cgatgcattt ggtgcaggta gctttatggt tgctacaaaa atcaaaggca
     4081 tgattgctgc tgaagtatca gatgaacgct ctggatatat gactagaggc cataacaatt
     4141 caagaatgat cactatggga tctgaaattg ttggagacac attagcgaaa aatgtcgtta
     4201 aaggttttgt tgaaggtaaa tacgatggtg gtagacacca aatccgcgta gatatgctta
     4261 ataaaatgtg ctaaggagga aaaattacta tgaagattgc attaggatgc gaccatattg
     4321 ttacagatac aaaaatgcgt gtatctgaat ttttaaaatc aaaaggacat gaagtcattg
     4381 acgtaggaac atacgatttt acaagaacac attatccaat ttttggtaaa aaagttggcg
     4441 aacaagttgt tagcggtaat gcagacttag gtgtttgtat ttgtggaaca ggtgttggta
     4501 ttaacaatgc tgtaaataaa gtacctggcg ttcgttcagc actagtacgt gatatgacat
     4561 cagcgttata tgctaaagaa gaattaaatg cgaacgttat tggctttggt ggacgtatta
     4621 taggtgagtt attaatgtgc gatattatcg atgcatttat taacgctgaa tataaagcaa
     4681 ctgaagagaa caaaaaatta atcgctaaaa ttaaacattt agaaacaagc aatgcagatc
     4741 aagctgatcc acatttcttt gatgaattct tagaaaaatg ggacagaggc gaataccacg
     4801 attaagggac gacacatatg attttaactt taacattaaa tccatccgtt gatatttcat
     4861 atccattaac agcattgaaa ttagatgatg ttaatcgtgt tcaagaagta agtaaaacag
     4921 ctggtggtaa aggtttaaat gtaacaagag tacttgcgca agtaggcgag ccagtccttg
     4981 cgagtggttt tattggcggt gaattaggtc aatttattgc taaaaaatta gatcatgccg
     5041 acatcaagca tgcattttat aacattaagg gtgaaacacg aaactgcatt gcaattttac
     5101 atgaagggca acaaacagaa attttagagc agggaccgga gattgataat caagaggctg
     5161 caggatttat taaacatttt gaacaattat tagaaaaagt tgaagcagtt gctatttcag
     5221 gtagcttacc taaaggatta aatcaagatt attatgcaca aattattgaa cgatgccaaa
     5281 acaaaggtgt gcctgtgata ttggactgct cgggtgcaac tttgcaaaca gttttggaaa
     5341 atccatacaa accaacagtc attaaaccaa acatttctga actatatcaa ttgcttaacc
     5401 agccacttga tgaaagttta gaaagtttga aacaggcagt gtcacaaccc ttattcgaag
     5461 ggattgagtg gattattgtg tctttaggtg cacaaggtgc attcgctaag cacaatcata
     5521 cattctatag agtgaatatt ccgacaataa acgtattaaa tccggttggt tcaggtgatt
     5581 ctacagttgc tggaattact tcagcaattc tcaatcatga aaacgaccac gatttactaa
     5641 aaaaagctaa tactttaggc atgctgaatg cgcaagaagc acaaacagga tatgtcaatt
     5701 taaataatta tgatgaatta ttcaatcaaa ttgaagtttt agaggtgtaa ataatgtcaa
     5761 aatctaatca aaaaatcgca tcaattgaac aattaagtaa taacgaaggt atcatttctg
     5821 cattagcatt tgaccaacgt ggtgctttaa aacgaatgat ggctaaacat caaacggaag
     5881 agccaactgt cgctcaaatt gaacaattaa aagtattggt tgcggaagaa ttaacacaat
     5941 atgcgtcttc aattttatta gacccagaat acggtttacc agcatctgat gcacgtaaca
     6001 aagattgtgg attattgcta gcttatgaaa aaactggtta tgacgtaaat gctaaaggtc
     6061 gtttacctga ctgtttagta gagtggtctg caaaacgttt aaaagaacaa ggtgcaaatg
     6121 ctgttaaatt cttactttac tatgatgttg atgatgctga agaaattaac attcaaaaga
     6181 aagcatacat tgaaagaatc ggttcagaat gtgttgcaga agacattcct ttcttcttag
     6241 aagtattaac atacgatgac aacattcctg ataacggtag tgttgaattc gcaaaagtta
     6301 agcctagaaa ggttaacgaa gcaatgaaat tattctcaga accacgcttt aatgttgatg
     6361 tattgaaagt tgaagtacca gtaaacatga agtatgtaga aggctttgct gaaggtgaag
     6421 tggtttatac aaaagaggaa gcggcacaac actttaaaga tcaagatgca gcaactcatt
     6481 taccatacat ttacttaagt gctggtgttt ctgcggaatt attccaagaa actttaaaat
     6541 ttgctcacga agctggtgct aaattcaacg gtgtactttg tggccgtgct acatggtctg
     6601 gtgctgttca agtatacatt gaacaaggtg aagatgctgc tcgtgaatgg ttacgtacaa
     6661 ctggtttcaa aaatattgat gacttaaata aagtattaaa agatactgcg acatcttgga
     6721 aacaaagaaa ataagtataa aaggggatta atgctatgaa tagagaagaa gtccaattat
     6781 taggttttga aattgttgca tttgcagggg atgcacgttc taagttttta gaagcattga
     6841 cagcagctca agctggagat tttgcaaaag cagatgcatt gattgaagaa ggaaacaatt
     6901 gcattgctga agcgcataga gcacaaacaa gcctgttagc taaagaagcg caaggtgatg
     6961 atattgcata cagtgtaacg atgatgcaaa aattgatcgc acaaattgaa aaagggaagc
     7021 catttttcga gaaattatca cgtaacatat atttaagagc catccgtgat ggatttattt
     7081 ctgcaatgcc tgtcatctta ttctcaagta tctttctatt aattgcatac gtgccaaata
     7141 tatttggttt taaatgggat aaaggcatgg aagccatttt aatgaaacca tataactata
     7201 cgatggggct tgtagcattt ttagtagcag gaacgacagc gaaatcttta acagattcat
     7261 ttaaccgtaa gctagaaagt acaaatcaaa ttaactttat ttctacaatg ctagcagcga
     7321 tgtgtggatt cttgttctta gcttctgatc cagccaaaga tggtggattc ttaagtgcat
     7381 ttatgggaac taaaggttta ttaacagcat ttttatcagc atttgtaact gttattgttt
     7441 ataacttctg tgtgaagcgc aatattacaa ttaaaatgcc gaaagaagta ccgccgaata
     7501 tttcacaagt atttaaggac ttaattccat tttcagcggt aatcatcatt ctttatgcat
     7561 tagatttagt cattcgcaac agctttaaat caaatgtagc ggaaggtatt ttaaaattat
     7621 tcgaaccatt atttacagca gcagatggat ggattggtgt cacaattatc tttggtgcct
     7681 ttgcattatt ctggtttgta ggaattcatg gtccatcaat agtagagcca gcaattgcag
     7741 ccattacata tgcgaatatc gaagcgaact tcaagttgct tcaagctgga gaacacgcag
     7801 ataaaattat tacatcaggt acacaaatgt ttatcgttac ttttggaggt acgggtgcaa
     7861 cattggtcgt cccgttcatg tttatgtgga tgacgaaatc taaacgaaac aaagcgattg
     7921 gacgtgcatc agttgtacca acattctttg gtgtaaacga accaatttta tttggtgcac
     7981 cactagtatt gaatcctgtg ttctttattc catttgtatt agcaccaatt gttaacgtat
     8041 ggattttcaa acttttcgtt gaagtgttag gaatgaatag ttttagtgta aatttacctt
     8101 ggacaacacc aggtccattg ggcattatca tgggtacagg ttttggttta tggtcattcg
     8161 tactagctat tactttgatt gttgtagata ttattattta ctacccattc ctaaaagttt
     8221 atgatagtga aattcttgat gaagaagaag gacgtaaaga aagtaattca gatttaaaag
     8281 aaaaagttgc agcaaacttt gatacgaaaa aagctgattc aattttagcg gcaagtggtg
     8341 tatcagacga tgcagctaaa gcttcaaata tcacagaaca aacaaatgtc ttagtacttt
     8401 gtgccggtgg tggtacaagt ggattattag ccaacgcact taataaagca gctgaagaat
     8461 atcatgtgcc agtgaaagca gcagctggcg gatacggcgc acatatggac attatgaaag
     8521 agtaccaatt aatcatttta gcgccacaag ttgcgtctaa ctatgaagat attaagcaag
     8581 atacagatag attaggtatt aaattagcga agactcaagg tgctgaatat atcaaattga
     8641 cacgagacgg ccaagcagcc cttgatttcg ttcaacaaca atttgaaaat taagaatagg
     8701 agtttttcat atgactaaaa cattacctga agattttatt tttggtggag caacagcagc
     8761 atatcaagca gaaggtgcga caaatacaga tggtaaaggt cgtgttgcat gggatacgta
     8821 tttggaagaa aactattggt acactgcaga accagcgagt gatttttata acagatatcc
     8881 agtggattta gaattaagtg aaaaattcgg tgttaatggt atccgtattt caattgcatg
     8941 gtcacgtatc ttcccaaatg gatatggcga agttaaccct aaaggtgttg agtattacca
     9001 taagttattt gcagagtgtc ataaacgtca tgttgaacca tttgtaacat tgcatcattt
     9061 tgatacacca gaagtgttac acaaagatgg cgattttcta aatcgtaaaa cgattgacta
     9121 ctttgtagat tatgctgaat attgttttaa agaattccca gaagtaaaat attggactac
     9181 attcaatgaa attggaccta ttggtgatgg tcaatattta gtaggtaaat tccctccagg
     9241 tattaaatat gattttgaaa aagttttcca atcacatcac aatatgatgg ttgctcatgc
     9301 aagagcagta aaattattta aagatggcgg ctatcaagga gaaattggtg ttgtacacgc
     9361 attgccaaca aaatatccgt tcgatccatc aaatcctgaa gatgttcgtg cagcagaatt
     9421 agaagatatc atccataata aattcatctt agatgcaaca tatttaggta agtattctcg
     9481 tgaaacgatg gaaggtgtgc aacatatctt atctgttaat ggcggtaaat taaatattac
     9541 agatgaagat tatgccattt tagatgcagc caaagattta aacgacttct taggtatcaa
     9601 ttactacatg agtgactgga tgagaggtta cgatggagaa tctgaaatca cgcataatgc
     9661 gacaggtgat aaaggcggtt ctaaatacca acttaaaggt gtcggacaac gtgaatttga
     9721 tgtcgacgta cctcgcacag actgggactg gatgatctat cctcaaggtt tatatgatca
     9781 aatcatgcgt gttgttaaag attatcctaa ctatcacaag atttatatca ctgaaaatgg
     9841 tttaggatat aaagatgaat ttattgaatc tgaaaaaaca gttcatgatg atgcacgtat
     9901 tgattatgta agacaacatt tgaacgttat tgcagatgct attaaagatg gtgcaaatgt
     9961 taaaggttac tttatttggt cattaatgga tgtgttctct tggtcaaatg gttatgaaaa
    10021 acgatatggt ttattctatg tcgattttga gactcaagaa cgttatccta agaaaagtgc
    10081 ttattggtac aaagagttag ccgaaacaaa agaaattaaa taattataga tattaaaagg
    10141 cctatcagtc gagtgctggt aggtctttat ttatagaaag agataaatta aacaagtata
    10201 tacatattta tatattaatc aatgggtgac acaccattga aagctaaaga agcaatcaat
    10261 taacaaacgt taacggacaa ctttccaaag tcatcttaaa agttaatgat gtattcaaaa
    10321 tcaactaaag gacattttta atcacatcct ttatacttat ataaaagaca tgaatgggag
    10381 ttaaaagtgt gaagaaaatt tttgtaatta ttacgacatt gttagcggtc gctattatta
    10441 taggctctat tattacggtg gtgtttagtc aacgacaggc acaaacattt aaaatacaac
    10501 aacagcaatt tgttaaaaag ccaattccaa cattgttctt acatggattt ggtggtagtg
    10561 ccaattctga aaagtttatg gtgaagcaag cagaaaaaag aggggtaact aaagatatca
    10621 tcactgctta cgtttcaaag gatggtgcag tgacatttaa agggaaacta agaaaagatg
    10681 cagtgaatcc tattgtgaaa attgaacttg aaaataacag acaaggctat ttggataaga
    10741 atgctgcgtg gtttaaaaat gtactgacta agttacaatc tgaatacaat ttcgataaat
    10801 tcaactttgt cggtcactca atggggaact taacatttgc acaatatatg atgacatatg
    10861 gtaacgacaa gtcactgcca cagttaaaca aacaagtgaa tattgcaggg acatttaatg
    10921 gtgttttaaa tatgaatgaa gatgtcaatg aaattacagt tgataaagat ggtaaaccaa
    10981 gtcgaatgaa tcaaccttat cagcaattac gcgtgctaaa agatatttat aaaggtaaag
    11041 gtatcgaagt gctaaatatt tatggagatt tgaaagatgg tacacattca gacggcagag
    11101 tgtctaatag ttcttcgaaa tcattaaaat atttactggg aaatagtccg aaaagttatc
    11161 gagaatctaa atatgaaggt gagcctgcac aacatagtca gcttcatgag aatgaaaatg
    11221 ttgctaatga gttaatcgac ttcttatgga aaaaataaag caactgctag ttttctgggg
    11281 caaaaaagct tgtaatatta gagcttttca aaaattatct attgtttgag agtttccata
    11341 aaatgtctaa gtcgtaacat ttttaattta tgtttacagc aaatataatg tacttgaagt
    11401 taaatgaatc agtgcttaag tcaaaatata acaactaaga atgtatttgc atacagacat
    11461 cacgaagaca caattaaaac ttaacgctta agtaacaaat aagtaaaagt ttcaaataaa
    11521 gaaaggtgat taatgatgca aaataaacaa attcttttta acaaaatacc agaaggcatg
    11581 cctcaagagg atacattcaa aattgaagag attgacacgc ctaaacttga gagcgatggt
    11641 gtacttgtac aaacgctgta tatttcagtg gatccatata tgagaggacg tatgactaag
    11701 gcagattcgt atgtacagcc ttttgaaatt ggtaaaccga ttgttagcca tgtcgttgca
    11761 aaagtaattg attcgacact cgcagattat aaaaaaggag atgtcgttgt aggtatgttg
    11821 ccttggcgaa tcattaatca tgtccaagcg gatcaaatta ctaaagttcc aacgacagat
    11881 gtaccacttg atttatattt aagtgtttta ggaatgccag gacaaacagc atatcatggt
    11941 ctgcttgata ttggaaaacc taaagccggt gatactgttg tggtttcagc tgcatctggt
    12001 gctgtaggtt cagttgtagg tcaaattgct aagattaaag gttgccgtgt agtaggtatt
    12061 gcgggtggcg acaaaaaagt aaattattta acagagacac tgggctttga cgctggtatt
    12121 gattataaga aagaggattt tgcagaagcg ttagcacaag cagtaccaga tggtattgat
    12181 gtttattttg aaaatgttgg tggtacagtt ggcgatgaag tatttaagca tttaaatcgt
    12241 tttgcacgtg taccagtatg cggcgcaatt tcgtcatata atcatcctga agcagatatc
    12301 ggaccacgta ttcaaggtac gcttattaag aagcaagtaa tgatgagagg cttcttagta
    12361 agtgaattcg ctaatgcttt taaagaagct agtgaacagc tcgcaacatg ggttcaagaa
    12421 ggtaaaattc agtcacaagt aaccattgaa gatggttttg aaaatgcgcc acatgcattt
    12481 aaaaatttat ttactggcga taactttggt aaacaagtta ttaaagtaac ggaataataa
    12541 agtatacgtt ttaacaaact aactttgtta attgatcaaa atttaataac tttcttatct
    12601 aaagcaaatt tgttcagtaa catggtagaa ataagtctaa aatgtaatac gaaagtcgtc
    12661 tgtaattatt tatatattca aatgaaaaag gagtcatgat gctgcggtat aagcacacat
    12721 ggcttctatt ttgtataata agaaataagt tcataaatga ggtgaaacga tatgaaaatg
    12781 ataggatttg aaaagccctt taaattggaa gaaggaaatt tatttaaagt atacgaacaa
    12841 agaaagccaa cgccagaaaa cgatgatatc ttagtcaaag taaatagtat tagtgtaaat
    12901 cctgtagata caaaacaacg tcaaatgaaa gtcactcaag cacctcgtgt acttgggttt
    12961 gatgctattg gtactgttga agctatcgga ccagacgtta cattatttag tccgggtgac
    13021 gttgtatttt atgctggatc gccaaataga caaggatcaa atgcgacata tcaattggtg
    13081 tcagaagcta tagttgcaaa ggcaccacac aacataagtg ctaatgaagc ggttagtcta
    13141 ccgctaactg gtattacggc atatgaaacg ttttttgata catttaaaat ttcacataat
    13201 ccttctgaaa atgtaggtaa atccgtgtta atcattaatg gtgccggtgg tgtaggcagt
    13261 atagccactc aaattgctaa acggtatggt ttaactgtca ttacaacagc atcacgacaa
    13321 gaaacaacag agtggtgtga aaagatgggc gcagatatcg tattgaatca taaagaggat
    13381 ttagtgcgtc aatttaaaga aaaagaaatt ccattagtag attatatatt ttgcacttat
    13441 aatacagact tgtattacaa tactatgatt gaactgataa aaccattagg gcatattacg
    13501 actatcgtag cttttaatga agatcaagat ttaaatgcat taaaattaaa aagtataaca
    13561 tttacacatg aatttatgtt tgcgcgtcca attcatcgaa cgcctgatat gattaaacaa
    13621 catgagtatt tagaagatat tacgaaaaat atagaattag ggcattacca accaacaacg
    13681 acacaagttt ttgaaggttt atcacctgaa aacttatatc aagcacatca attgttggaa
    13741 aaacaatcaa tgattgggaa attagtaatt aatatataga ttagtaagta aagtcgcctt
    13801 atgcagaaaa tactgtatag ggcgacttat tttttgtgtc tagcgactat tgattctaaa
    13861 cggaaatatg gcaactttac aggtatcaaa tcaataatgc actagaaagg cattacttaa
    13921 atacaataaa atactttgtt gctcaatgat aaataatttg agtgtgtttt atatatcatt
    13981 tttaaaagat gaggcattat taattgtgtc tatataaaat tacaaatgat taaaatgttg
    14041 cagttatggg tatgaactta ccattacaca gaaagggttt gtatttatga aaaataaatc
    14101 agtaagtcta gtgttttggg tatctttagt gatatgtacg atatttgttg cttttggagc
    14161 aatatttccg aaacaattag aaaaactcac gcaaaacatt actacattta ttgcgctaca
    14221 tttttcttgg tattatttat tgctcgtttt agttatttta tttgtctgtg tgtatatttt
    14281 attttcgaga tacgcaaaca ttacattagg tgaagaaggc gaagatcctg aattctcatt
    14341 gccgtcttgg tttgctatgt tatttagtgc tggtatggga ataggacttg tgttctggac
    14401 aacggcagaa ccaatcagtc atgccttcaa attaacgcct attcataaag ctggcacgca
    14461 atctgcaatt aatgatgcaa tgcagttttc atttttccat tgggggattc atgcatgggc
    14521 agtctatggt attgtcgcgc ttgtgtttgc atattttagc tttcataaag gatatcctgg
    14581 cttagtcagc gctacattga cgcctttatt aggtgaaaag gcaatgcgtg gacctttagg
    14641 tggtgccatt gatgtattgg cagtcatagc tacagtaaca ggcgttgctg caacattagg
    14701 tttcggtgca ttgcaaataa acgaaggttt acatttttta tttaatgtac cgagtaactt
    14761 cacgatgcaa gtaatcttga ttgtcattgc aaccatatta tttacttggt cagcatggtc
    14821 aggtatcgat aaaggtatca aaacgttgag taatatcaac atgttacttg cttttgtagt
    14881 acttattggt ctttttattg ttgggccaac gctttacatt ttaaatacat ttacaaatgg
    14941 tctaggtaat tatattgcaa atttctttag tatgagttta cgtattccgt caggcggtca
    15001 aaaattccaa tggttacaaa attggacaat cttttactgg gcatggtgga tatcttgggc
    15061 accatttgta ggtatattta ttgcacgtgt gtcaaaagga cgtacgatta aagaatttat
    15121 tctaggtgta ctttttgtcc cggcgttagt ttgctttata ttctttgcgg tattcggcgc
    15181 gtcagcaatt tacttacaag ataatcatat tgccgatatt gctaaagcag cgacagaaac
    15241 agcaactttt gcaacgctac aacattaccc actaggtttt gtattaagct taattacttt
    15301 acttgttatt atgattttct ttgttacatc agcggattcg gcaacttatg ttttaggtat
    15361 gttgagttca agaggtgata ttaatccgaa gtcatttgtc aaagtgagtt ggggtatcat
    15421 tatggcatta tttgcaatta tcatgatcta cacaggtggt actcaagcga ttcaaaattt
    15481 attaatcatt gctgcgttac cattttccgt ggtcattata gcgatgatat ggtcgctttt
    15541 aaaatcttta agtgaagaaa aacctagaaa tagtaataag gttttaatta aacatcgtga
    15601 cccagatatt ttagaatatc gttcttcaaa acatattgac gaaaattaat taataatgag
    15661 gttttatt
//
LOCUS       DAOBBH010000044        15453 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.044, whole genome shotgun sequence.
ACCESSION   DAOBBH010000044 DAOBBH010000000
VERSION     DAOBBH010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15453
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.044"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            19..594
                     /locus_tag="P6L01_001046"
     CDS             19..594
                     /locus_tag="P6L01_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDP3030127.1"
                     /translation="MSKGLKLSEILVTVLISVVFAVIYNLWWFVYNGVQATGLHLEQL
                     TNGVWFMAAIVCYLIIPKPGIALLAEFAAGAGETIIMGRFDIPTIVYAFIQGLACELV
                     FAIFKYQSRSVMVAMLAGFCTAIAAFPIDYFYGYLNEVAGWNLTLFIVFRLISGAVVA
                     GVLSYLLVKALDKTGVTKLFRPAAKEDYDNL"
     gene            609..2009
                     /locus_tag="P6L01_001047"
     CDS             609..2009
                     /locus_tag="P6L01_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3030128.1"
                     /translation="MLKVSDLRLKYPNGQRKIFDHLNITIQDKEKVLLLGPSGCGKST
                     LLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPDSQFCMPKVYEELAFVLENRQVP
                     REDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
                     DVQATEDLWTKLIELWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQ
                     KYVHLLSEYGVWHPRAWEFAPSRVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGL
                     GEWITITGANGSGKTTLLESIMQLIKYQGDVYFENQRLTKIKHAAKHMYLVYQNPELQ
                     FITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLQNVKDQHPYELSIGQKRRLSVAT
                     ALSSKADIIFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRL
                     KISDGALLDCDGDTNV"
     gene            2002..2808
                     /locus_tag="P6L01_001048"
     CDS             2002..2808
                     /locus_tag="P6L01_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDP3030129.1"
                     /translation="MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYIT
                     ILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTES
                     LVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLM
                     ISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRD
                     GPRTYYYNVPFSYKDIIFIIVLIAIITMAYYCSITLPITGIDDVRLGRVG"
     gene            complement(3075..4322)
                     /locus_tag="P6L01_001049"
     CDS             complement(3075..4322)
                     /locus_tag="P6L01_001049"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDP3030130.1"
                     /translation="MNVLVIGAGGREHALAYKLNQSNLVKQEFVIPGNEAMTPIAEVH
                     TEISESNHQGILDFAKQQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEG
                     SKLFAKKIMKKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
                     EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHD
                     EGPNTGGMGAYCPVPHISDDVLKLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDG
                     PKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWKNESIVGVMLASKGY
                     PDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVQDAQRDAYEK
                     VSQIQSDHLFYRHDIANKALQLK"
     gene            complement(4344..5822)
                     /gene="purH"
                     /locus_tag="P6L01_001050"
     CDS             complement(4344..5822)
                     /gene="purH"
                     /locus_tag="P6L01_001050"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDP3030131.1"
                     /translation="MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVP
                     VRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLY
                     PFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYHEVLTRLRNDS
                     LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIA
                     GAKQLHGKQLSYNNIKDADATLALVKKFDTPAAVAVKHMNPCGVGIGDTIEQAFQHAY
                     EADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEI
                     DMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPS
                     VKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVEL
                     AAQHGIKAIIQPGGSIKDQDSIDMANKHGIAMVVTGTRHFKH"
     gene            complement(5837..6403)
                     /gene="purN"
                     /locus_tag="P6L01_001051"
     CDS             complement(5837..6403)
                     /gene="purN"
                     /locus_tag="P6L01_001051"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDP3030132.1"
                     /translation="MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFC
                     IDRAKKHDIPVYINEPKQFDSKAAYEQHLVTLLNKDKVEWIILAGYMRLIGPDLLASF
                     EGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDCGMDTGEIIEQRQCDIRP
                     DDSKEQLEEKVKKLEYELYPSVIAKIVK"
     gene            complement(6406..7434)
                     /locus_tag="P6L01_001052"
     CDS             complement(6406..7434)
                     /locus_tag="P6L01_001052"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDP3030133.1"
                     /translation="MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGAT
                     FDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYF
                     LDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGA
                     VEKDDYVDGSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVF
                     LEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIF
                     DWLQQQGNIDTNEMYNIFNMGIGYTVIVDEKDASRALKILAEQNVEAYQIGHIVKNES
                     TAIELLGV"
     gene            complement(7427..8911)
                     /locus_tag="P6L01_001053"
     CDS             complement(7427..8911)
                     /locus_tag="P6L01_001053"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDP3030134.1"
                     /translation="MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIV
                     VSDQNELKGERGLGLLTEAINDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHF
                     YDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
                     LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQ
                     DIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKK
                     LAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQ
                     GVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFP
                     SFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVE
                     SFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHFFDSEGNLNV"
     gene            complement(8890..11079)
                     /gene="purL"
                     /locus_tag="P6L01_001054"
     CDS             complement(8890..11079)
                     /gene="purL"
                     /locus_tag="P6L01_001054"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDP3030135.1"
                     /translation="MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTET
                     GIFSVMWSEHCSYKHSKPFLKQFPTSGEHVLMGPGEGAGVVDIGDNQAVVFKVESHNH
                     PSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGG
                     YGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGVGNSVIYVGLKT
                     GRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGA
                     AGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGNEQKF
                     LDLFDKHELDSAVIGEVTDTNRFVLTYDDEVYADIPVEPLADEAPVYILEGEEKDYNT
                     SKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTN
                     KAIASTIDGEARYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIY
                     QQLIDSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPVVGMVGLIENVNYLNDF
                     EPQVGDKLYLIGDTKDDFGGSQLEKLIYGKVNHEFESLDLSSEVEKGESIKTAIREGL
                     LSHVQTVGKGGLLITLAKLSAHYGLGLKSSIDITNAQLFSETQGRYVVSVKSGKTLNI
                     DNAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD"
     gene            complement(11072..11743)
                     /gene="purQ"
                     /locus_tag="P6L01_001055"
     CDS             complement(11072..11743)
                     /gene="purQ"
                     /locus_tag="P6L01_001055"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HDP3030136.1"
                     /translation="MKFAVLVFPGSNCDRDMFNAAIKSGVEAEYVDYRETSLSGFDGV
                     LIPGGFSFGDYLRSGAMASVAPIISEVKRLATEGKPVLGVCNGFQILTEIGLLPGALL
                     HNDSHLFISRNEELEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQQLKANN
                     QIILKYVNNPNGSYDDIAGIVNEKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSW
                     REQHV"
     gene            complement(11745..12008)
                     /gene="purS"
                     /locus_tag="P6L01_001056"
     CDS             complement(11745..12008)
                     /gene="purS"
                     /locus_tag="P6L01_001056"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDP3030137.1"
                     /translation="MKTIELHITLQPQVLDTQGQTLTRAVHDLGYAQVNDIRVGKVLY
                     MTVDEVSDEKVHNIITTLSEKLFANTVIEEYSYKVLDDEKENA"
     gene            complement(12008..12712)
                     /locus_tag="P6L01_001057"
     CDS             complement(12008..12712)
                     /locus_tag="P6L01_001057"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDP3030138.1"
                     /translation="MTLLYEGKAKRIFSTNQENELRVEYKDEVTAGNGAKKDTMAGKG
                     RLNNQITSIIFKYLQENGIESHFIKQLSETEQLVKPVKIIPLEVVVRNIASGSITKRL
                     GFENGEVFREPLVEFFYKNDALNDPLITDDHVKLLNIASDEDIEILKSKALKINNVLK
                     QLMDAMNLKLVDFKIEFGKTETGQILLADEISPDTCRIWDKATNANFDKDVYRNNTGS
                     LIETYQIFLNKLEDLK"
     gene            complement(12716..13840)
                     /gene="purK"
                     /locus_tag="P6L01_001058"
     CDS             complement(12716..13840)
                     /gene="purK"
                     /locus_tag="P6L01_001058"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDP3030139.1"
                     /translation="MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDC
                     PCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAI
                     QLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQV
                     LINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITFFPLQENEHRNQI
                     LFKTIVPARIDKTAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSG
                     HYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWH
                     LHIYGKSGRKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN"
     gene            complement(13827..14309)
                     /gene="purE"
                     /locus_tag="P6L01_001059"
     CDS             complement(13827..14309)
                     /gene="purE"
                     /locus_tag="P6L01_001059"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDP3030140.1"
                     /translation="MKVAVIMGSSSDWKIMQESCNMLDYFEIPYEKQVVSAHRTPKMM
                     VQFASEARERGINIIIAGAGGAAHLPGMVASLTTLPVIGVPIETKSLKGIDSLLSIVQ
                     MPGGIPVATTAIGAAGAKNAGILAARMLSIQNPSLVEKLNQYESSLIQKVEDMQNELQ
                     "
     gene            14510..15370
                     /gene="folD"
                     /locus_tag="P6L01_001060"
     CDS             14510..15370
                     /gene="folD"
                     /locus_tag="P6L01_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDP3030141.1"
                     /translation="MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDG
                     ASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPK
                     QVSEQKILEAINPEKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEAKN
                     AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPSLVTKD
                     VVKEGAVIIDVGNTPDENGKLKGDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLL
                     AEKMRRGIDS"
BASE COUNT         4794 a         2976 c         2295 g         5388 t
ORIGIN      
        1 tattaggagg atgtaaaaat gtcaaaaggt ttaaagctat ctgaaatact agtaaccgta
       61 cttatttcag tagtattcgc agttatttac aatttatggt ggtttgttta caatggtgtg
      121 caagctacag gccttcacct cgaacaatta acaaacgggg tttggttcat ggcagcgatt
      181 gtctgctatc taattattcc aaaaccaggt attgctttac tggctgaatt tgctgcaggt
      241 gccggcgaaa ctatcattat gggtcgtttc gatattccga caatcgttta cgctttcata
      301 caaggtttag cgtgtgaact tgtctttgca atttttaaat atcaatctcg ttcagttatg
      361 gtagctatgt tagcaggttt ttgtacagca atcgcggctt tccctataga ttatttctat
      421 ggttatttaa atgaagttgc aggttggaac ttaacattat tcatcgtatt tagattaatt
      481 agtggtgcag tagtcgccgg cgtcctatct taccttttag taaaagcttt agataagaca
      541 ggcgtaacaa aattatttag accagctgct aaagaagatt acgacaactt ataagaggag
      601 cattcagagt gttaaaagta agtgatttac gattaaaata tccaaatggt cagcgtaaaa
      661 ttttcgatca tttaaatatc actattcaag acaaagaaaa agtactttta ctcggtcctt
      721 ctggttgcgg taaaagtaca cttctgaatg tattaagtgg tattgttcct aatttaattg
      781 aattacctat gaaatatgat gaactaatcg ttgacccatt aagtggcgtt attttccaag
      841 accctgatag ccagttttgt atgccaaaag tatacgaaga acttgcattc gttttagaaa
      901 atagacaagt accacgtgaa gacatggatg cgttaattat caatgcttta aatatggtca
      961 atttaaatgt tacccctgaa acgtatatca aagatttaag tggcgggatg aaacagaaat
     1021 tggcaattgt tgaaaccatt cttcaacaat caaaaacatt gtttttagat gaaccgacag
     1081 caatgttaga tgttcaagca acagaagatt tatggactaa actaattgaa ctttgggaag
     1141 atcaaactgt tgtaatcgtt gaacataaag ttaaacacat ctggaatcat gtcgaccgcg
     1201 tcattttgat ggattataac ggaaatatca ttgccgatga atgtcctgaa atcatattac
     1261 agaagtatgt tcatttactc agtgaatatg gtgtgtggca tccacgtgca tgggaattcg
     1321 caccaagtcg tgttgacttt ccaacaacaa actcacactt attacaattt aaaaatggac
     1381 gtattattcg cggtaaatca acattgctct cattctcaga tttagaaatt ggtctaggtg
     1441 agtggattac aattacaggg gcaaatggta gtggtaaaac aaccttgctt gaatcaatta
     1501 tgcaattgat taaatatcaa ggtgatgttt attttgaaaa tcagcgttta acaaaaatta
     1561 aacatgcagc aaaacacatg tacctagttt atcaaaaccc agaattacaa tttataacaa
     1621 attcggttta tgatgaaatt aacattcatt ttaatcacct ttctaaagat caaagtgatg
     1681 atgaaacgat acaactttta aaacttttag atttacaaaa tgtaaaagat caacatcctt
     1741 atgagttgtc tattggtcaa aaacgacgcc ttagcgtagc taccgcacta agttctaaag
     1801 ctgatattat ctttttagat gaaccgacat ttggacttga tagccataat acattccagt
     1861 tgatcaaact tttccaaaag cgaattaatt tgggacaaag tattgtcatg gttacacatg
     1921 acgatgaaat aattgaacgt tatccatcaa gaagacttaa aatttcagat ggtgcgttac
     1981 tagattgtga tggtgatacg aatgtatgag caatggaaag ttcggcaatc atttgttgat
     2041 gatgtcaaca ttataacgaa actatttctt ggaattatac tatttttctt tattatattt
     2101 gtgcataatt tcgattttat gctttatatc actatcttaa tgttagtttt cttattgatg
     2161 tttaatggta ctcagtttaa aattacagca atttttataa ctataagtat tggctttgca
     2221 ttactctctt ccctgttcat gattttatac gggaatggta gccatacaat tttgaaattc
     2281 ggatttatcc atattacaac agaaagttta gttcgtggtt tacatgtatc attaagaacc
     2341 attgctattt catttttcgg tatcttaatc gcacttacat ctcaggttgt catgatattt
     2401 tatagtttaa tgcaacattt aaaggttaaa tcaaaagttg cttatgcatt catggctgcc
     2461 attcgtatga tacctttaat gattagttct ttaatacagc ttcgcagatc tttaaaaatg
     2521 cgctatcaga tgatagacgc agcaaattat agagggttaa agagatttaa gcatttaatt
     2581 atacctctat taagccaaaa tattagaaag gcacatcaat tatcagttgc gatggagtcg
     2641 aaaggattta gagatggtcc tcgtacttac tattacaacg tacctttctc atataaagat
     2701 ataatcttta ttatagtttt aattgccatt attactatgg catattattg ttcaatcacc
     2761 ttacccatca cgggcataga tgatgttcgt cttggtcgtg taggttaaat tttatccata
     2821 attgtataga tactaaatta gaaattcatt gtatagaaaa agaaaaccat ttattccata
     2881 ctaaacaaaa gaggtgccac aaacatgtta ttttgttcat ccaaacatgt tgtggcgcct
     2941 cttttgtatt acatatgcat aatcacaaat ttggtacaga aatcaatgtc ctagactcta
     3001 caactaaaaa agctatgaaa aaaactatcg ttatagattt cttcatagct aatcttagta
     3061 ttttttaaat atacttattt aagttgtagt gctttattcg caatgtcatg acgatagaat
     3121 aaatggtcac tttgtatttg agatactttt tcgtatgcgt ctcgctgtgc atcttgtaca
     3181 ttgtcacctt ttccgatggc aagtataact ctaccacctg aagtaacaaa ggtatcacct
     3241 tgcttcttta atccactaac aaaatagttt tcatttaaat caaagccact tactttatgc
     3301 cctttttcat atgcatcagg atatcctttt gatgccaaca tgacccctac aatagattca
     3361 tttttccatt tgaattcagt acgttttcct tcatctaaat caataatatg ctgcattaaa
     3421 tcactttcca tgcgacttaa taatacttga gcttcaggat caccaaaacg ggcattaaat
     3481 tctattactt ttggaccatc tttagttaaa atagcaccaa tgtataatac accgaagaat
     3541 tgataacctt cattaagcat tgcctttgca atgggttgtg caattgtttc atttgtaagt
     3601 tttaaaacat cgtcactaat atgtggtact ggacaataag cccccatacc accagtattt
     3661 ggtccttcat catgatcaaa tgcgcgttta tgatcttgtg caatacagtc gaaaggtact
     3721 gctaaatcac cattaacaaa tgtcattagc gagaactctt caccttctaa aaacgtttca
     3781 aatacaacag taccttcttc ttcatcacca tacataatct caatagcact tctggctgct
     3841 tcaatagtat ctgcaataat aacgcctttc ccagcagcta acccatcttt cttgacaaca
     3901 acgggcaatt cacagttttc aatatatgtt aaagcatcct tttttcgctc aacttcttta
     3961 taatcagcag ttggaatatt atattttttc attatctttt tagcaaataa ttttgagcct
     4021 tcgatttgag ctgcttgctt atttggacca aacactttga aaccattcgc tcgtaaaatg
     4081 tctgctaatc catcaattag cggctgttct ggacctataa ctacccaatc aacattttgc
     4141 tgtttagcaa aatctagtat cccttgatga ttagattctg aaatttcagt gtgtacttca
     4201 gctataggtg tcattgcctc attacctgga atgacaaact cttgtttaac tagattcgat
     4261 tgattaagtt tatatgcaag tgcatgttct cgtccaccag caccaattac taatacattc
     4321 atttatgtta cgccccttta aaattagtgt ttaaaatgtc gagtgcctgt gactaccatt
     4381 gcaataccat gtttattagc catatcaatt gaatcttgat ctttaatcga accacccggt
     4441 tggataattg cctttatacc atgttgtgct gcaagttcaa ctgtatctcc catagggaaa
     4501 aatccatcag atactaacgc tacatgatca ttgatttcaa tagctctctc taacgcaatt
     4561 ttagcagcac cgacacgatt catttgtcca gcacctatac ctacagtttg tttattatta
     4621 cttaaaataa ttgcattact ttttactgat ggtacaactt tccatcctaa taacattgct
     4681 tcccattgtt catcagtagg tgctacttct gtaacaactt tcatttcttc ttttggcacg
     4741 acataattgt ctttatcttg aactaaatat ccgccagata ctgaaacaaa ctcttcttcg
     4801 ttactgtcta tagtcatatc aatttctaat aatcttacat tcttcttttg ttttaaaata
     4861 tctaatgctt catctgtaaa ttttggtgca ataatgactt ccaaaaagat actatgcaat
     4921 tgctctgcta actcaggtgt tacagctcgg tttaatgcaa caattccacc aaatattgat
     4981 tgactatccg cttcatacgc atgttgaaat gcttgttcaa tcgtgtcacc gataccaaca
     5041 ccacatggat tcatgtgttt aaccgcaact gcagcaggtg tatcaaactt tttaactaaa
     5101 gctagtgtag catctgcatc tttaatattg ttatagctta attgtttccc atgtaattgt
     5161 ttagcacctg caatcgtgtg cttagcattc gaagttctca caaaatacgc tgattgttgt
     5221 ggattttctc catatcttaa agtttcttta tcccctttaa agaaacgtac aatcgcttca
     5281 tcatattctg cagtatgctc aaaaacttta atcattaatg attgtctata tgactcatct
     5341 aacgaatcgt ttcttaatcg cgtcaatact tcatgataat ctgccggatg tacaattgtt
     5401 gttacatgtt tatagttttt agctgcagca cgtaacattg ttggaccacc aatatcaata
     5461 ttttcaattg cttcgtccat cgtcacatca gggtttgcaa cagtttgttg gaatggatat
     5521 aaattaacta ctaccatatc aattaaatct atatgttgtt ctgataattc atttaaatgc
     5581 tgcggtttat ttcgatcagc taaaatgcca ccatgaacag ccggatgtaa tgttttaaca
     5641 cggccatcca ttatttctgg aaaatgtgtt aagtctgaaa cagaacgaac tggtacattt
     5701 gcttcatcta atatacgttt agtaccacca gttgaatata attcataatt taattgcgtt
     5761 aacgctttag caaactctac aatacctgtt ttatttgata cgctcaaaat agctttcttc
     5821 atcacataca tactccttat tttacaattt tagcaataac acttggatat aactcatatt
     5881 ccaatttttt tactttctct tctaattgtt cttttgaatc gtccggtcta atatcacatt
     5941 gtctctgttc aataatttct cccgtatcca taccacaatc aacataatgt actgtcgaac
     6001 cagtaatagt atcgccacta tgatatgctt ggcctattgc gtcaatcccc ttatatttcg
     6061 gcaatagaga tggatgtata ttcaatattt taccttcaaa tgaagctaat aaatctggac
     6121 caattagacg catgtagcca gctaaaataa tccactctac cttatcttta tttaataatg
     6181 ttactaaatg ttgttcatac gctgcttttg aatcaaattg ttttggttca ttaatataaa
     6241 caggaatatc gtgctttttt gctctatcta tacaaaacgc attttgatga tccgtatata
     6301 gcgccgtaac ttcaatattt tcaagttttc ctgattcaac atgctcaact atattttcaa
     6361 agttacttcc tgaacctgat gcaaaaatcg caattttaac cattgttata cccccaacaa
     6421 ttcaattgca gttgactcat ttttcacaat atgaccaatt tgataagctt ccacattttg
     6481 ttctgctaaa atcttcaaag cgcgtgatgc atctttttca tcaacgataa ccgtatagcc
     6541 aatacccatg ttaaaaatgt tatacatttc atttgtgtct atattgcctt gttgttgtaa
     6601 ccaatcaaat atttttggcg ttggaaatga tgtagtatca attctagcag catatccggc
     6661 tggcaatgca cgtggaatat tttcataaaa acctccacca gtaatatgat tcattgcctt
     6721 aatagaaact tcttttttta aagcaagtac aggtttgaca tataatttag ttggttctaa
     6781 aaagacatct ataaatggac gattatcgaa gtttgatgcc aaatcaatgc ctgattcatt
     6841 aattaatttg cgcactaaac tgtatccatt tgaatgaatg ccacttgacg caagtcctat
     6901 aacaacttgt ccctctttca cttctgaacc atctacatag tcatcctttt caactgctcc
     6961 aacagcaaat ccggctacat catattcacc ttcgtgatac atttcaccca tttcagcagt
     7021 ctctccaccg ataagtgcag tattcgtttc aacgcatgca tcactaatac ctttaacaat
     7081 ttgttcaata acttcaggaa caactttatt tgtagcgatg taatctaaaa aatacaatgg
     7141 ttctgcacct gtcgttaaaa tatcattaac acacattgca actgcatcga tacctatcga
     7201 atcatgtttc ccatagtcga tagctagttt taatttcgta cctactccgt ctgttccaga
     7261 aactaaaact ggcgctgtca tatttaattg tgataaatca aatgtagcac cgaatccacc
     7321 taaaccaccg ataacttctt tacgcatcgt acgtttaaca tgactagaca ttctttctac
     7381 agcttcataa ccagcatgaa tatttacacc agattgttca tatgctttag acatttaaat
     7441 ttccctcgct atcaaaaaag tgtttgtttt tagaaatata ttgcttttgt cgatgactta
     7501 aatgtgcttt ataatttgct tcataatcat ataaccctgc aggataatct ccagtgaaac
     7561 tttctacaca taagccacta tatggcgcgt cataatctaa accaattgat tcaattaacc
     7621 catctacaga tagatatgct aatgaatcag cgccaatata atctttaatt tcttcaggtg
     7681 atttgcttgc agaaattaat tctgccgtag ttgaaacatc gattccataa aaacttggga
     7741 acataaattc cggtgatgct atacgcacat gcactttatt tgcaccagaa tcttttaaca
     7801 ttttcacaat gcgtcgaatt gtcgtaccgc gaacaatgga atcatcaaca agaatgatat
     7861 ttttcccatc tactatatct tttaccgcag ataacttcac tctcacacct tgctcacgta
     7921 attcttgagt tggttgaata aatgttcttg caacatattg atttttaact agtcccattt
     7981 catatggcaa acctatttct tcagcataac cactcgcagc tgatagcgat gaattgggta
     8041 caccgatgac catatcagca tttacagggc tttcttgggc taatttttta ccagaagctt
     8101 tacgtactgc atggacattt ttaccagcta ttgttgagtc tggtctagca aaataaatat
     8161 attccatcgc agaaattgca gttgtcgtat gatgtgtata agatttaact gtaatacctt
     8221 tatcgttaat cacgacatat tcacctgcat gaatatcttg aacaaattct gcacctaaca
     8281 catctattgc acatgtttca cttgcaagga tgtatgtacc atctttcatt ttacctacaa
     8341 caagtggtct gatagcattt ggatctactg cgccatataa cgcatcttta gttaatatcg
     8401 caaatgtaaa accgccttta acttttcgca aactttcttt caatgcttcc tcaaaagtag
     8461 gagctttact tcgacgtatc aaatgcataa tgacttcagt atctgaagac gaatggaaga
     8521 tagcaccttg tttttctaaa ttctgacgca atgatttagc attaatgagg ttaccattat
     8581 gacaaatacc tacactcata tcataaaagt gatacagaaa cggttgaata ttttcaatac
     8641 ctttattacc tgaagtagca taacggacgt gaccaattgc atgttgatat ccttttaatc
     8701 gttccatttg atcatcgtta atcgcttcag ttagtaagcc taatcctcgc tcgcctttta
     8761 attcattttg atcagaaaca actatacctg caccttcttg accacgatgt tgcaaactat
     8821 gaagtcccat atatgttagt tgcgctgctt caggatgatt ccaaatacca aacacgccac
     8881 attcttcgtt taatcctgag tagttaaaca ttgagcaatt gccccttccc atatttgttt
     8941 aatatctgaa acattttcac taatctctgt atatggtgtt gttactttga aattatcact
     9001 atctgttaaa agtccaattt ctattgcatt atcaatattt aaagttttac ctgatttaac
     9061 agaaacaaca tatcggcctt gtgtctcact aaacaattgt gcatttgtta tatctattga
     9121 agattttaat cctaaaccgt aatgcgcact tagtttagct aaggtaatca gtaagccacc
     9181 tttaccaact gtttgaacat gtgataatag tccttcacga atagcggtct tgattgattc
     9241 acctttttca acttctgaac tcaaatctaa tgactcaaat tcatgattaa ctttgccata
     9301 aattaacttt tcaagttgac taccaccaaa gtcgtcctta gtatcaccga ttaaatataa
     9361 tttatctcca acttgaggtt caaaatcatt taaataattt acattttcaa tcaaacctac
     9421 cattccaaca actggtgttg ggaaaataga agtacctttc gtttcgttat ataaagatac
     9481 atttccagaa actactggtg tcttaagaat gtcgcatgct tctgccatac ctttcgttga
     9541 atctatcaac tgttgataga tttctttctt ttcaggagaa ccataattta aacaatctgt
     9601 cattgctaat ggtgttgcac ccacggcaat taaatttcga taagcttcag ctactaccat
     9661 ctttccacct tcatatggat tgttatatac ataacgcgct tcaccatcaa ttgttgaagc
     9721 aattgcctta tttgtgcctt ccacacgtac taccgatgct tgaagtcctg gcttaattat
     9781 cgtattggca ccaacttgtt ggtcgtattg atcatataaa tagtgtttag atgctatagt
     9841 cggatgctta agtaatttaa agaaagtatc tttaacatcg atgtgtgtat aatcattttt
     9901 agaagtatta taatcttttt cttctccttc taaaatatat acaggtgctt catcagctag
     9961 tggttcaact ggaatgtcag cataaacttc atcatcatat gttaaaacaa aacgatttgt
    10021 atctgtaact tcacctataa cagcactatc caattcgtgc ttatcaaata aatctaagaa
    10081 tttttgttca ttaccttttt caacaactag taacatacgt tcttgagttt ctgaaagcat
    10141 catttcataa ggagaaatac ctggctcacg tgttggcact tgttctaatc tcaaatgtaa
    10201 cccactacca ccttttgccg ccatttcaga cgatgaagat gttaaaccag cagcacccat
    10261 atcttgaata ccaactaatt catcaaatgt aattgcttca agtgttgctt ccattaattt
    10321 tttacctaca aatggatcac cgatttgtac agaaggtcgt ttactttcgc tttcttccgt
    10381 caattcttca gatgcaaaag tagcaccatg aataccatct cgaccagttt tcaaaccaac
    10441 ataaatgacc gaattaccta caccttttgc tgtgcctttt tgaatcatgt cgtgattgat
    10501 aacaccaaca cacattgcat taacaagtgg attgccatca taacgttcat caaattcgat
    10561 ttcaccagca gttgttggaa taccaatgca gttaccataa cctccgatac cctttacaac
    10621 ccctttaagt aatctttggt tttgtttatt atctaattct ccaaatctaa gactgtttaa
    10681 caaattaata ggtctagccc caatagaaac aatgtcacga atgattccac caacgcctgt
    10741 agcagcccct tgatatggtt caattgctga tggatgattg tgagactcta ctttaaatac
    10801 tacggcttga ttatcaccta tatcgactac ccctgcacct tcacctggac ccataagcac
    10861 atgctcacct gacgtaggaa attgctttaa aaacggttta gaatgtttat aagagcaatg
    10921 ttcactccac ataacagaaa agatacctgt ttctgtaaag ttaggttgtc tgcctaaaat
    10981 atcgcaaact ttttcatatt cttgatcact taatcccata tcttgatata ctttttcaag
    11041 tttaatttct tcaacgcttg gttcgataaa tttagacatg ttgttccctc caacttttta
    11101 ccatcgcttc aaataatttc acaccactat cagtacctaa caacgtttct aaagctcttt
    11161 caggatgtgg catcatgcca catacattgc ctttttcgtt aacaattcct gcaatatcat
    11221 catatgaacc gttcggatta ttcacatatt tcagaataat ttgattgtta gcttttaatt
    11281 gttgatatat ttcatcagta caataataat gaccttcacc gtgagctaca ggatatataa
    11341 ctttttcacc ttgctcataa agatttgtaa atgccgtttg attattcact atttctaact
    11401 cttcatttct actaataaat aaatgtgaat cgttatgcaa taatgcacca ggtaataagc
    11461 ctatttcagt taaaatttga aacccattac aaacacctaa tactggctta ccttcagttg
    11521 caagacgttt aacttccgaa ataatcggcg ctacactagc cattgcccca gatcttaagt
    11581 aatccccgaa tgaaaatcca ccaggaataa gtacgccatc aaatccactt agtgatgttt
    11641 ctctataatc tacatattcc gcttcaacac cacttttaat agcagcatta aacatgtctc
    11701 tatcacaatt cgaacctgga aaaacaagaa ccgcaaattt cattttatgc attctccttt
    11761 tcatcatcta acactttata gctatattct tcaatcactg tatttgcaaa caatttttca
    11821 cttagagttg taataatgtt gtgtaccttt tcatcactaa cctcatccac tgtcatatat
    11881 aatacttttc ctacacgaat atcattcact tgtgcataac ctaagtcatg tacagctcga
    11941 gtaagcgttt gtccttgcgt atctaatact tgtggttgta atgtgatatg tagttcaatt
    12001 gttttcatta ttttaaatcc tccaatttgt ttaaaaatat ttgatatgtt tcaatcagtg
    12061 atccagtgtt atttctatat acatctttat caaagtttgc attggtagct ttatcccaaa
    12121 ttcgacatgt atctggagat atttcatccg ctaacaaaat ttgaccagtc tcagtctttc
    12181 caaattcgat tttaaaatct actaatttta aattcatagc atccattaat tgtttcaaca
    12241 cattattaat ctttaatgct ttggatttta gtatttcaat atcttcatct gatgctatat
    12301 tgagcaattt aacatggtca tccgttatca acggatcatt taacgcatca tttttataga
    12361 aaaattctac aagtggttct ctaaaaactt caccattttc aaaacctaaa cgctttgtaa
    12421 tagatccact agcaatatta cgaacaacta cttctaatgg aattattttc acaggcttaa
    12481 ctaattgttc tgtttcagat aattgtttaa taaagtgact ttctattcca ttttcttgta
    12541 aatatttaaa tataatagaa gtaatttgat tatttaatcg ccccttacct gccattgtgt
    12601 ctttcttagc cccgtttcca gcagtaactt catctttata ttcaactctt aattcatttt
    12661 cttgatttgt tgagaaaatg cgcttcgctt ttccttcata taataatgtc atgctttaat
    12721 tactcccctc aaatttagcg tacatatctt gttcagtttg gtttacatca ttcgttagta
    12781 cagtcatatg ccccattttt ctgctatctt tacgcccaga cttaccataa atatgtaagt
    12841 gccactctgg atgttcatta aattcatttt ccaataaatc taagtcttta cctagtaagt
    12901 tcatcatgac tgcaggcttt aataattcaa ttgaatttgg taatgattgt ccggtaactg
    12961 ctaaaatatg agtatcaaat tgtgaataat cacatgcttc aattgaataa tgtccggaat
    13021 tgtgaggcct tggtgcaatc tcgttcacat acaattggtt gttactatct ataaaaaatt
    13081 ccactgtaaa tgttccaatg aaatgaatcg attggataat tttattaact tgctctttcg
    13141 cctcagctgt tttatctatt ctcgctggaa caattgtttt gaaaagtatt tgatttctat
    13201 gctcattttc ttgtaatggg aaaaaagtga tttgattgtt gtttcctctt gtaacagtaa
    13261 gagatacttc tttcttgata ttcaaatatt tttcagctac gcattcacta gtttcaatta
    13321 atttaaaacc ttcttgtaag tctttttcgt tgttaattaa aacttgacct ttgccatcgt
    13381 agccaccaaa tctagttttt acaataaaag gatatcctaa tgtttcaatt gctttgtcaa
    13441 tatctgtaga ttcttttact gaaatgaacg ggacaacttt ggtaccagca ctttttaatg
    13501 tttctttttc agttaagcgg tcttgtaata actgtatagc ttggtaacct tgcggaatat
    13561 tgtacttttc acataatagt tttaattgtt gggctgaaat gttttcaaat tcataagtaa
    13621 tcacatcaca tttttgtcct aattgattga gtgccttttc atcgtcatac ttggcttgta
    13681 taaattcgtg tgcaacgtat ctacatggac aatcttcaga aggatccaat acaaccactt
    13741 tataacccat tttttgagct gattgtgcca tcatctttcc aagctgacca ccaccaataa
    13801 tgccaatagt cgcaccaaac tttaatttat tgaagttcat tttgcatgtc ctccactttt
    13861 tgaattaacg aagattcata ctgatttagt ttttcaacta aagaaggatt ttgaatactt
    13921 aacattcttg ctgcaagtat acctgcgttt ttagcacctg ctgcaccaat tgcagtcgtt
    13981 gcaaccggaa tacctcctgg catttgaaca attgataata aagaatctat accctttaaa
    14041 ctttttgttt caatcggcac accaataact ggtagcgtcg ttaatgatgc aaccatacct
    14101 ggtaaatgtg ccgcaccgcc agcgcctgca atgataatgt ttatacctct ttctctcgct
    14161 tcagaagcaa attgaaccat catttttggc gtacgatgtg cggatactac ttgtttttcg
    14221 tacggaattt caaaataatc caacatgtta caactctctt gcataatttt ccaatcggaa
    14281 gaactgccca taatgactgc tactttcact ttgtacaccc tttcaaaagt ttgaattgtg
    14341 aattacttta gttgtatatt atagatatag cataacaagc aatttctgct ttttcaatca
    14401 aaaatcgaac tttattttga ttttttgttt gaatttacgt cttttgctat gtaaattagt
    14461 tttataaact aacaaagtta ggatattgac aataggagga gaagttttta tggttgctaa
    14521 aattttagat ggtaaacaaa ttgccaaaga ctacagacag gggttacaag atcaagttga
    14581 agcgctaaaa gaaaagggtt ttacacctaa attatccgtt atattagttg gtaatgatgg
    14641 cgctagtcaa agttatgtta gatcaaaaaa gaaagcagct gaaaaaattg gtatgatttc
    14701 agaaatcgta catttggaag aaacagctac tgaagaagaa gtattaaacg aactaaatag
    14761 actaaataat gatgattctg taagtggtat tttggtacaa gtaccattac caaaacaagt
    14821 tagcgaacag aaaatattag aagcaatcaa tcctgaaaaa gatgtggacg gatttcatcc
    14881 aataaatata gggaaattat atatcgatga acaaactttt gtaccttgca caccgctcgg
    14941 catcatggaa atattaaaac atgctgatat tgatttagaa gctaaaaatg cagttgtaat
    15001 tggacgaagt catattgtcg gacaaccagt ttctaagtta ctacttcaaa aaaatgcatc
    15061 agtaacaatc ttacattctc gttcaaaaga tatggcatca tatttaaaag atgctgatgt
    15121 cattgtcagt gcagttggta agcctagttt agtaacaaaa gatgtggtca aagaaggagc
    15181 agtaattatc gatgttggca atacgccaga tgaaaatggc aaattaaaag gtgacgttga
    15241 ttatgatgcg gttaaagaaa ttgctggagc tattacacca gttcctggtg gcgttggtcc
    15301 attaacaatt actatggtat taaataatac tttgcttgca gaaaaaatgc gtcgaggtat
    15361 tgattcgtaa agagcctgag acataaatca atgttctatg ctctacaaag ttataatggc
    15421 agtagttgac tgaacgaaaa ttcgcttgta aca
//
LOCUS       DAOBBH010000045        15090 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.045, whole genome shotgun sequence.
ACCESSION   DAOBBH010000045 DAOBBH010000000
VERSION     DAOBBH010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15090
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.045"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            4..1548
                     /gene="miaB"
                     /locus_tag="P6L01_001061"
     CDS             4..1548
                     /gene="miaB"
                     /locus_tag="P6L01_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDP3030142.1"
                     /translation="MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKR
                     GKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDI
                     NTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
                     SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVN
                     IMYGCDKFCTYCIVPFTRGKERSRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDL
                     QDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGN
                     NAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEV
                     GFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRLNKKVGHYSQIAMSKYEGQTVT
                     VLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEVRIDEAKQYSLNGSFVKEVE
                     PEMVIQ"
     gene            1549..1914
                     /locus_tag="P6L01_001062"
     CDS             1549..1914
                     /locus_tag="P6L01_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
                     /protein_id="HDP3030143.1"
                     /translation="MYNKDDVLKQADNIANKIKNLDTIKTYQQIEAQIHQNQTIKTKM
                     DMLKKHQKQAVNFQNYGKQNALEQSEHTIQSIEAEINTLPIVEQFQTSQYEANQLLKM
                     FVSTMETRLNDHNKAKHSD"
     gene            1941..2432
                     /gene="thiW"
                     /locus_tag="P6L01_001063"
     CDS             1941..2432
                     /gene="thiW"
                     /locus_tag="P6L01_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HDP3030144.1"
                     /translation="MKSRKLAITALLIAINVVLSSIIIIPLGPVKAAPVQHFVNVLSA
                     VIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEV
                     LGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIISYFLLITLKKRGILQR
                     FIK"
     gene            2735..5353
                     /gene="mutS"
                     /locus_tag="P6L01_001064"
     CDS             2735..5353
                     /gene="mutS"
                     /locus_tag="P6L01_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDP3030145.1"
                     /translation="MSNVTPMMQQYLKIKSEYQDCLLFFRLGDFYEMFYEDAKEASRV
                     LEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRR
                     EVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATL
                     LNEITTINPNEVVINDNISDNLKRQINMVTETITVRETLSSEIYSVNQTEHKLMYQAT
                     QLLLDYIHHTQKRDLSHIEDVVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWL
                     MDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIE
                     RLVGRVSYGNVNARDLIQLKHSISEIPNIKALLNSMNQNTLVQVNQLEPLDDLLDILE
                     QSLVEEPPISVKDGGLFKVGFNTQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKIS
                     FNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAI
                     ELEYQLFVQLREEVKKYTERLQQQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLE
                     LVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQM
                     GAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDE
                     IGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALPSLKNVHVAANEY
                     KGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLK
                     MVENEPEINQENLNLSVEETTDTLSQKDFEQASFDLFENDQESEIELQIKNLNLSNMT
                     PIEALVKLSELQNQLK"
     gene            5366..7375
                     /gene="mutL"
                     /locus_tag="P6L01_001065"
     CDS             5366..7375
                     /gene="mutL"
                     /locus_tag="P6L01_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDP3030146.1"
                     /translation="MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEIS
                     IEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALAS
                     ISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYI
                     KSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVAR
                     DLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLT
                     IGRFPICYINIEMDPILVDVNVHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKN
                     NLDYVPKKNKVLYSFEQQKIEFEQRQNTENNQEKTFSSEESNSKSFMAENQNDEIVIK
                     EDSYNPFVTKTSESLITDDESSGYNNTREKDEDYFKKQQEILQEMDQTFDSNEDASVQ
                     NYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAA
                     QERIKYEYFRDKIGEVTNEVQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGG
                     HDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHY
                     LQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM"
     gene            7381..7923
                     /locus_tag="P6L01_001066"
     CDS             7381..7923
                     /locus_tag="P6L01_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HDP3030147.1"
                     /translation="MNQVNNNILPAIRNIKDLEKLIKTDYKMCVLLDMHIGHIKSIME
                     LLKQNHIECFIHIDLIKGLSHDEFASEFIIQQYKPKGIVSTKSKVIKKAKSLNTLTIF
                     RVFIIDSQALKRSIDLIKKVEPDFVEVLPGVASKAIHHIQKETNTQVIAGGLINTIDE
                     VNEAVKNGAKYVTTSYDKLW"
     gene            8398..9216
                     /locus_tag="P6L01_001067"
     CDS             8398..9216
                     /locus_tag="P6L01_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDP3030148.1"
                     /translation="MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGW
                     GLAVTMGVFAVGQFSGAHLNPAVSLALALDGSFDWSLVPGYIVAQMLGAIVGATIVWL
                     MYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
                     NPLIVGALIVAIGLSLGGATGYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILG
                     PIAGGLLGAVVYAVFYKHTFNIGCAIAIVVVIITLILGYILNKSSKKGDIESIY"
     gene            9345..10841
                     /gene="glpK"
                     /locus_tag="P6L01_001068"
     CDS             9345..10841
                     /gene="glpK"
                     /locus_tag="P6L01_001068"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDP3030149.1"
                     /translation="MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWV
                     EHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVW
                     QSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLL
                     FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKP
                     SSEIYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFECGDVKNTYGTGGFMLMNTGD
                     KAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATR
                     VDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVME
                     AMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAFLAGLAV
                     GFWESKDDIVKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE"
     gene            10999..12672
                     /locus_tag="P6L01_001069"
     CDS             10999..12672
                     /locus_tag="P6L01_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HDP3030150.1"
                     /translation="MALSTFKREHIKKNLRNDEYDLVIIGGGITGAGIALDASERGMK
                     VALVEMQDFAQGTSSRSTKLVHGGLRYLKQFQIGVVAETGKERAIVYENGPHVTTPEW
                     MLLPMHKGGTFGKFSTSIGLGMYDRLAGVKKSERKKMLSKKETLAKEPLVKKEGLKGG
                     GYYVEYRTDDARLTIEVMKRAAEKGAEIINYTKSEHFTYDKNQQVNGVKVIDKLTNEN
                     YTIKAKKVVNAAGPWVDDVRSGDYARNNKKLRLTKGVHVVIDQSKFPLGQAVYFDTEK
                     DGRMIFAIPREGKAYVGTTDTFYDNIKSSPLTTQEDRDYLIDAINYMFPSVNVTDEDI
                     ESTWAGIRPLIYEEGKDPSEISRKDEIWEGKSGLLTIAGGKLTGYRHMAQDIVDLVSK
                     RLKKDYGLTFSPCNTKGLAISGGDVGGSKNFDAFVEQKVDVAKGFGIDEDVARRLASK
                     YGSNVDELFNIAQTSQYHDSKLPLEIYVELVYSIQQEMVYKPNDFLVRRSGKMYFNIK
                     DVLDYKDAVIDIMADMLDYSPAQIEAYTEEVEQAIKEAQHGNNQPAVKE"
     gene            12822..13736
                     /locus_tag="P6L01_001070"
     CDS             12822..13736
                     /locus_tag="P6L01_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDP3030151.1"
                     /translation="MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERY
                     DKLAHALSKHGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKP
                     YIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYSLWKGLPTVKVLQLITKIYGAEK
                     RVEWVNQLVSNSFNKKIRPLRTQSDWISSNPIEVDNFIKDPYSGFNVSNQLLYQTAYY
                     MLHTSQLKNMKMLNHAMPILLVSGYDDSLGDYGKGILKLANIYRKAGIKNVKVNLYHH
                     KRHEVLFEKDHDKIWEDLFKWLNQFYKK"
     gene            13754..14689
                     /gene="miaA"
                     /locus_tag="P6L01_001071"
     CDS             13754..14689
                     /gene="miaA"
                     /locus_tag="P6L01_001071"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDP3030152.1"
                     /translation="MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYK
                     HMNIGTAKVTPEEMDGIPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAG
                     GTGLYIQSLIYNYELEDETVTPAQLSIVKQKLSALEHLDNQQLHDYLAQFDAVSAENI
                     HPNNRQRVLRAIEYYLKTKKLLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVD
                     IMLDHGLFREVQQLVEQGYESCQSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYA
                     KRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK"
     gene            14704..14937
                     /gene="hfq"
                     /locus_tag="P6L01_001072"
     CDS             14704..14937
                     /gene="hfq"
                     /locus_tag="P6L01_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDP3030153.1"
                     /translation="MIANENIQDKALENFKANQTEVTVFFLNGFQMKGVIEEYDKYVV
                     SLNSQGKQHLIYKHAISTYTVETEGQASTESEE"
BASE COUNT         5677 a         1999 c         2883 g         4531 t
ORIGIN      
        1 aaagtgaacg aagaacaaag aaaagcaagt tctgtagatg ttttagctga gagagataag
       61 aaagcagaaa aagattatag taaatatttt gaacatgttt atcagccgcc taatttaaaa
      121 gaagcgaaaa aaagaggtaa acaagaagtt cgttataata gagatttcca aattgatgaa
      181 aaatatcgcg gtatggggaa cgagcgtaca tttttaatta aaacatatgg atgtcaaatg
      241 aatgcacatg acactgaggt cattgctggt atacttgaag cattaggcta tcaagcaacg
      301 actgatatta acactgcaga tgttatttta attaatacat gtgcgattag agaaaatgcc
      361 gagaacaaag tgtttagtga aataggtaat ttgaagcatt tgaaaaaaga acgacctgat
      421 attttaatcg gtgtttgtgg ttgtatgtca caagaagagt cagtagtgaa taaaatttta
      481 aaatcgtatc aaaatgtaga tatgatattt ggtacacata atattcatca tttaccagaa
      541 attttagaag aagcatactt atctaaagca atggttgttg aagtatggtc taaagaagga
      601 gacgttattg aaaatcttcc aaaagtccgt gaaggcaaca ttaaagcatg ggtcaatatt
      661 atgtatggtt gtgataagtt ttgtacatat tgtattgttc catttacaag aggtaaagaa
      721 cgaagccgta gacctgaaga cattatagat gaagtacgtg aacttgctcg tgaaggttac
      781 aaagaaataa cgcttttagg tcaaaatgta aattcttatg gtaaagattt acaggatata
      841 gaatatgact taggagatct tttacaagca atttctaaaa tagcgattcc aagagttcgt
      901 ttcacaacaa gtcatccttg ggactttaca gatcacatga ttgatgttat ttcagagggt
      961 ggtaatatcg ttcctcatat ccacttgcca gttcaatctg gaaataatgc agtattaaaa
     1021 ataatgggta gaaaatatac acgagaaagt tatttggatt tagtaaaacg aatcaaagat
     1081 agaattccta atgtagcatt aactacagat attattgtag ggtatccaaa tgaatcagag
     1141 gaacaatttg aagaaacttt aactctgtat gatgaagttg gttttgaaca tgcatatacg
     1201 tacttgtatt cacaacgtga tggtacgcct gctgctaaaa tgaaagataa tgtaccttta
     1261 aatgtcaaaa aggaacgatt gcaacgtttg aataaaaaag ttggtcatta ttcacaaata
     1321 gctatgagta agtacgaagg acaaactgta acagtacttt gtgaaggtag tagtaaaaaa
     1381 gatgatcagg ttcttgctgg ctacactgat aaaaataagc tagttaattt caaagcacct
     1441 aaagaaatga ttggtaaact agtggaagta cgaatagatg aagctaaaca gtattcatta
     1501 aatggcagtt ttgtaaagga agtagagccg gaaatggtga ttcaataaat gtataataaa
     1561 gatgacgtgt tgaaacaagc ggataatatt gcaaataaaa ttaaaaattt ggatactatc
     1621 aaaacatatc aacaaattga agcacagatt catcagaacc aaacgataaa gactaaaatg
     1681 gatatgttaa aaaagcatca aaaacaagca gtaaactttc aaaattacgg gaaacaaaat
     1741 gcgctagaac agtcggaaca taccattcaa agtatagaag cagaaataaa tacattgccc
     1801 atagttgaac agtttcaaac ttcacaatat gaagcgaatc aattattgaa aatgtttgta
     1861 tcaacaatgg aaacacgttt aaatgaccat aataaagcca agcatagtga ttaattacaa
     1921 acaaaaagga gaacaatcat atgaaatcaa gaaaactggc tataactgca cttttaattg
     1981 caataaatgt tgtattaagc agtattatca tcattcctct aggaccagtt aaggcagcac
     2041 cagtacagca ttttgtaaat gtattaagtg cggtcatagt aggtccttgg tatggattag
     2101 ctcaagcgct tatatcatca attttaagag ttctttttgg tactggtaca gcttttgcat
     2161 ttccaggtag tatgattgga gttttattgg ctagtatgtt ttacatatat cgtaaacata
     2221 tattcatggc cgcggtcggt gaagtacttg gaactggtgt catcggaagt ttaatttgta
     2281 taccattagc atatttcctt gggcttcaag acttcttcat taaaccgcta atgattacgt
     2341 tcatagtctc aagtgctatc ggatctatta taagttattt cttattaatt actctaaaaa
     2401 aacgtggtat tcttcaaagg tttataaaat aattttatga tatatgattt caaacagagt
     2461 cattgttcat tttaatattg aacgccctta cttaattaaa tgtcttgaat gctagaattt
     2521 taatttaatt ctaaacatgc tattgacaca ttttattagg tttgaggcgt tttcttatac
     2581 atttaagatg cttcgtgatt atatggtatg aataggtttg taaaagtaag tatgttaaaa
     2641 tgtataagtt aaataattaa taattagaaa gcttacaaac gaatatattg taaagacaaa
     2701 ttttaaaaag agacaagggt tgtgaagaaa taatatgtct aatgttacac caatgatgca
     2761 gcaatattta aaaataaaat cagaatacca agattgctta ttatttttta gactaggtga
     2821 tttctatgaa atgttttatg aagatgccaa ggaggcatca cgtgtacttg aaattacttt
     2881 aactaaaaga gatgctaaaa aagaaaatcc aattccgatg tgtggtgttc cgtatcattc
     2941 tgcagatagt tatatagata cacttgttaa taatggatat aaagtagcta tttgtgaaca
     3001 gatggaagat ccgaaacaaa cgaaaggtat ggttagacgt gaggtagtaa gaattgtgac
     3061 tccaggaact gtgatggagc aaggtggtgt agatgataaa caaaataact atattttaag
     3121 ttttgttatg aatcaacctg aaattgcgct tagttactgt gatgtttcta ctggcgaatt
     3181 aaaggttaca cattttaatg atgaagcgac tttattaaat gaaattacga cgataaaccc
     3241 taacgaagtt gttatcaatg acaatatttc cgataattta aaaagacaaa ttaatatggt
     3301 gacagaaaca ataacagtca gggaaacgtt atcatcagaa atctatagtg tgaatcaaac
     3361 tgaacataaa ttaatgtatc aagcgacaca attattgcta gattatattc atcatacaca
     3421 aaaacgtgat ttatcgcata tcgaggatgt tgttcaatat gcagctatag attatatgaa
     3481 aatggatttt tatgctaaga gaaaccttga gttaacggaa agcattcgat taaaatcaaa
     3541 aaaaggaacg ctactttggc taatggacga aacgaaaaca ccaatgggag cacgccgctt
     3601 aaaacaatgg atagatagac cactaataag taaagaacaa attgaagcac gattagatat
     3661 cgttgatgaa tttagtgctc atttcataga aagagacacc ttaagaacat atcttaatca
     3721 agtgtatgat attgaacgtc ttgttgggcg tgttagttac ggaaatgtta atgcgagaga
     3781 tttaattcaa cttaaacatt ccatttctga aataccgaat attaaagcat tactaaattc
     3841 tatgaatcag aatacgcttg tacaagttaa tcaactagaa ccccttgatg atttacttga
     3901 tatattagaa cagagtttag tagaagaacc accaatttca gttaaagatg gcggactatt
     3961 caaagttggt tttaatacgc aattagatga atatcttgaa gcttcaaaaa acggaaaaac
     4021 atggttagca gaattacaag ccaaagaaag acaacgtaca ggaataaaat cattgaaaat
     4081 aagctttaat aaagtgtttg gttattttat agaaataaca cgtgccaact tgcaaaattt
     4141 tgaaccaagt gaatttggtt atatgaggaa gcaaacgtta tcgaatgctg aacgttttat
     4201 aactgatgaa cttaaagaaa aagaagatat cattttaggt gcggaagaca aagccatcga
     4261 attagaatat caattatttg ttcagctacg tgaagaagtt aaaaaatata ctgaacgttt
     4321 acaacaacaa gctaaaatta tttcagagct agattgttta cagagctttg cagaaattgc
     4381 tcaaaaatat aattacacta ggccttcatt tagtgaaaat aaaacattag aattagtgga
     4441 atctaggcac ccagtagtgg aaagagtaat ggattataat gactatgtgc ctaataattg
     4501 tcgattagat aatgaaacat ttatatattt aattacaggt ccgaatatgt ctggtaaatc
     4561 gacatatatg agacaagttg ccataattag tataatggcc caaatgggag cttatgtccc
     4621 ttgtaaagag gcagtgttac ctatatttga tcaaatattc actagaatag gtgcggcaga
     4681 tgatttggtt tcaggtaaga gtacgtttat ggtagaaatg ctagaagcac aaaaggcatt
     4741 aacttatgca acagaggata gtttgattat tttcgatgaa attggacgtg gtacttcaac
     4801 gtatgacggt ttagctttag cgcaggcaat gatagagtat gtagctgaaa catcgcatgc
     4861 taaaacgtta ttttcaacac attatcatga attgacaaca ttagatcaag cattaccaag
     4921 tctaaaaaat gttcacgtcg ctgctaatga atataaaggt gaacttatat tcttgcataa
     4981 agtcaaagat ggtgcagttg acgatagtta tggtattcaa gttgcgaaat tagctgattt
     5041 acctgaaaaa gttattagca gagcacaagt gatcctaagc gagtttgaag cgtctgctgg
     5101 taaaaaatca tcgatatcaa atttaaaaat ggtcgaaaat gaacctgaaa ttaatcaaga
     5161 aaatttaaac ttaagtgttg aagaaacaac tgatacttta tctcaaaaag actttgaaca
     5221 agcatcattt gatttgtttg aaaatgatca agaaagcgag attgaactac aaattaaaaa
     5281 tttgaattta tctaatatga caccaattga ggcattggtg aagttaagtg aattacaaaa
     5341 tcaattaaaa tagaggtgtt gcaaaatggg gaaaattaaa gaactccaaa cctcattagc
     5401 aaataaaatc gcagcaggtg aagtagttga aagaccgagt tctgttgtga aagaactgtt
     5461 ggaaaatgct atagatgcag gcgctacaga aataagcatt gaagtagagg aatctggcgt
     5521 ccaatctatt cgcgtagtcg ataatggaag cggaattgaa gcggaagact taggattagt
     5581 atttcataga catgcgacta gtaaattaga tcaagatgaa gatttatttc atattaggac
     5641 attaggattc cgtggtgaag cactagccag tatttcatca gttgctaaag taacattgaa
     5701 gacttgcacg gataatgcta atggaaatga aatatatgta gaaaatggtg aaatattaaa
     5761 tcataagcct gcaaaagcga aaaaaggaac agatatactt gtagaatcat tattttataa
     5821 tacaccagca cgtttaaaat atattaaaag tttatacact gaactaggta aaataacaga
     5881 tattgtcaat agaatggcta tgagccatcc ggacattcga atagcactca tttcagatgg
     5941 caaaacaatg ttaagtacaa atggttcagg acgaactaat gaagtgatgg cagagattta
     6001 tgggatgaaa gttgcacgag atttagtaca tatatctgga gatacaagtg attatcacat
     6061 tgaaggtttt gttgcaaagc ctgaacattc tagaagtaat aagcactata tttctatttt
     6121 tattaatgga cgatacatta aaaactttat gctaaataaa gcgattttag aaggctatca
     6181 tacactctta acaataggta ggttcccgat ttgttatatt aatattgaaa tggatccaat
     6241 cttagtagac gtaaatgttc atccaacaaa actagaagtg cgtttatcaa aagaagagca
     6301 actatatcaa ttgatagtga gcaaaataca agaagcattt aaagaccgta tattaattcc
     6361 taaaaataac ttggattatg tgccaaaaaa aaataaagta ttatattcat tcgaacaaca
     6421 aaaaatcgaa tttgaacaaa gacaaaacac agagaataac caagagaaga cgttttcatc
     6481 tgaagaaagt aacagtaagt catttatggc agaaaatcaa aacgatgaga tagttataaa
     6541 agaagattca tataatccat tcgtaacgaa aacatctgaa agtttaataa ctgatgatga
     6601 atcttctggt tataataata cacgtgaaaa agatgaagac tacttcaaaa aacaacaaga
     6661 aattttacaa gaaatggatc aaacatttga ttcaaatgag gatgcatctg tgcaaaatta
     6721 tgagaataaa gcgtctgatg attattatga tgtaaacgat attaaaggaa caaaaagtaa
     6781 agaccctaaa cgaagaattc catatatgga aattgttggc caagtacatg ggacgtatat
     6841 tattgctcaa aatgaatttg gcatgtacat gattgaccag catgcagctc aagaaagaat
     6901 aaaatatgaa tattttcgag ataaaatagg tgaagttacc aatgaagtac aagatttatt
     6961 aatcccgtta acatttcatt tttcaaagga tgaacaatta gttattgatc aatataaaaa
     7021 tgagcttcaa caagtaggta tcatgttaga acattttggt ggtcatgatt atattgtaag
     7081 tagctatcca gtttggttcc ctaaagatga agtagaagaa atcattaaag atatgattga
     7141 gctaattttg gaagagaaaa aagtagatat taaaaaatta cgtgaagatg tagcaatcat
     7201 gatgtcatgt aaaaaatcta ttaaagcgaa tcattattta caaaaacatg aaatgtctga
     7261 tttaattgat caattaagag aagcggaaga tccatttaca tgtccacatg gtcgtccaat
     7321 aatcattaat ttttcaaaat acgaattaga aaaattattt aagcgtgtga tgtagagagg
     7381 atgaatcaag tgaataacaa catattgcct gctataagaa acattaaaga tttagagaaa
     7441 ctgattaaaa cagattataa aatgtgtgtg cttctagata tgcatatagg acatataaaa
     7501 agtattatgg aattgctgaa gcaaaatcat atagagtgtt ttattcatat agatttgata
     7561 aaaggtttaa gccacgatga atttgcaagt gaatttatta ttcagcaata caagccaaaa
     7621 ggtatcgtat cgactaaatc taaagtaata aaaaaagcta aatcattaaa tactttaacg
     7681 atttttagag tatttattat tgatagtcaa gcattgaaac gcagtataga tttgataaaa
     7741 aaagtggaac ctgattttgt tgaagtactt ccaggtgttg cgagtaaagc gattcatcat
     7801 attcagaaag aaacaaacac acaagtcatt gcaggtggcc taattaatac aatagatgaa
     7861 gtcaatgaag ctgttaaaaa tggagcgaaa tatgtaacaa ctagttatga taaactttgg
     7921 taaatcataa gtctgcaaaa atttcatttt tcacgagatt tagtcatata acataataaa
     7981 aaagccgttt tcatatggga tatgtaataa ggtatttaag gtatcataca aagaataatt
     8041 aaataacata attagcacct ctaaaacgat taaaatcatt aaagttttag aggtgtttat
     8101 tagtaaaaaa tgcaactagt cataaaaaat atggctgaag aatgtcaatt aaacaaaatg
     8161 gcgtcataaa taaattagtg cggattgata tttctagttt tcaaattaaa ctacttttat
     8221 aaagacttga aaaattaaat ttgacaaaat ttttacaaaa accattgaca acgctttcat
     8281 atcgatagta tcataacaaa tataataaaa gttaatacat agaatagaga cgggagattt
     8341 ctacgagcca aactgctagt gtaggaatct ctttgtcttt ttgggaggac atttaatatg
     8401 aatgtatatt tagcagaatt cctaggaact gcaatcttaa tcctttttgg tggtggcgtt
     8461 tgtgccaatg tcaatttaaa gagaagtgct gcgaatggtg ctgattggat tgtcatcaca
     8521 gctggatggg gattagcggt tacaatgggt gtgtttgctg tcggtcaatt ctcaggtgca
     8581 catttaaacc cagcggtgtc tttagctctt gcattagacg gaagttttga ttggtcatta
     8641 gttcctggtt atattgttgc tcaaatgtta ggtgcaattg tcggagcaac aattgtatgg
     8701 ttaatgtact tgccacattg gaaagcgaca gaagaagctg gcgcgaaatt aggtgttttc
     8761 tctacagcac cggctattaa gaattacttt gccaactttt taagtgagat tatcggaaca
     8821 atggcattaa ctttaggtat tttatttatc ggtgtaaaca aaattgccga tggtttaaat
     8881 cctttaattg tcggagcatt aattgttgca atcggattaa gtttaggcgg tgctactggt
     8941 tatgcaatca acccagcacg tgatttaggt ccgagaattg cacatgcgat tttaccaata
     9001 gctggtaaag gtggttcaaa ttggtcatat gcaatcgttc ctatcttagg accaattgcc
     9061 ggtggtttat taggtgcagt ggtatacgct gtattttata aacatacatt taatattggt
     9121 tgtgcaattg caattgttgt agttattatt actttgattt taggttacat tttaaataaa
     9181 tcatcaaaaa aaggtgatat cgaatcaatt tactaaaata aaaagaaacg taaatagcat
     9241 aatttaacat gtttgattca tggattatgc tattttttcg ccaaaattta acagattttg
     9301 tacaatgggt tagcgattat tttttaataa aggagatact actaatggaa aaatatattt
     9361 tatctataga ccaaggaaca acaagctcaa gagcgatttt attcaatcaa aaaggggaaa
     9421 ttgcaggggt agcacaacgt gagtttaagc aatattttcc acaatcaggt tgggttgaac
     9481 atgatgcaaa tgaaatttgg acatctgtgt tagctgtaat gacggaagta attaatgaaa
     9541 atgatgttag agctgatcaa attgcaggaa tcggtattac aaaccaacgt gaaacaacgg
     9601 ttgtttggga caaacatact ggccgcccaa tttatcacgc aattgtttgg caatcacgtc
     9661 aaacacaatc aatttgttca gaattaaaac aacaaggata tgaacaaaca tttagagata
     9721 agacaggatt acttttagat ccgtattttg caggtacaaa agttaaatgg attctagaca
     9781 atgttgaagg tgcacgagaa aaagcagaaa atggcgatct attatttgga acgattgata
     9841 cttggttagt atggaaatta tcaggaaaag ctgcgcatat tactgattat tcaaatgcga
     9901 gtcgtacatt aatgtttaat atccatgatt tagaatggga cgatgagtta ttagaactac
     9961 ttacagtacc taaaaatatg ttgccagaag ttaaaccttc aagtgaaata tatggtaaga
    10021 caattgatta ccacttctat ggtcaagaag taccaatcgc tggtgtagct ggtgatcaac
    10081 aagcagcatt atttggacaa gcttgcttcg aatgtggtga cgtgaaaaac acatatggaa
    10141 ctggtggctt catgttaatg aatacaggtg acaaagcggt taaatctgaa agtggtttat
    10201 taacaacaat tgcttatggt attgatggaa aagtaaatta tgcgcttgaa ggttccatct
    10261 ttgtttcggg ttcagcaatc caatggttac gtgatggatt aagaatgatt aattcagcac
    10321 cacaatcaga aagttatgcg acacgagttg actctactga gggtgtttat gttgttccag
    10381 cttttgtagg tttaggaaca ccatattggg attctgaagc acgtggtgcg attttcggtt
    10441 taacacgtgg aactgaaaaa gagcacttta tccgtgcaac tttagaatca ttatgttacc
    10501 aaactcgtga cgttatggaa gcaatgtcaa aagactctgg tattgatgtc caaagtttac
    10561 gtgtcgatgg tggtgcagtt aaaaataact ttattatgca gttccaagca gacattgtta
    10621 atacttctgt tgaaagacct gaaattcaag aaactacagc tttaggtgct gcatttttag
    10681 caggtttagc agttggattc tgggagagta aagatgatat cgttaaaaac tggaaattag
    10741 aagaaaaatt cgatccgaaa atggatgaag gcgaaagaga aaaattatat agaggttgga
    10801 aaaaagctgt tgaagcaaca caagttttta aaacagaata aacttgtaga ttagactttt
    10861 gtataaacat tgtgatacaa tcaatttaag ttaatatttg aatcgagaaa cgagagattt
    10921 gttcgaacat gtacaattga aggattgttt atgtgggaca agtctctcgt ttttacattt
    10981 tttaggaggc gttttggaat ggcattgtct acttttaaga gagaacatat taaaaagaat
    11041 ttaagaaatg atgaatatga tttagtaatt attggtggcg gtattacagg tgcaggtatt
    11101 gcactagacg cgagtgaaag aggaatgaaa gttgcattag ttgaaatgca agactttgca
    11161 caaggaacaa gctcaagatc tacaaaatta gtccatggtg gtttacgtta cttaaaacaa
    11221 ttccaaattg gagtagttgc cgaaactggt aaagaacgtg cgattgttta tgaaaatggg
    11281 cctcatgtta cgactccaga gtggatgctt ttaccaatgc ataaaggtgg aacatttggt
    11341 aaattctcaa catcaattgg tttaggaatg tatgatcgtt tagcaggtgt taagaagtct
    11401 gaacgtaaaa aaatgttatc taaaaaagaa actttagcta aagaaccatt agttaaaaaa
    11461 gaaggtctaa aaggcggcgg ttactatgtt gaatatcgta ctgacgatgc gcgtttaact
    11521 attgaagtta tgaagcgtgc tgctgaaaaa ggcgcagaaa ttatcaacta tactaaatct
    11581 gaacacttca cttatgataa aaatcaacaa gtaaatggtg ttaaagttat agataaatta
    11641 actaatgaaa attatacaat taaggctaaa aaagtggtta atgcagcagg cccatgggtt
    11701 gatgatgtta gaagtggtga ttatgcacgc aataataaaa aattacgttt aactaaaggt
    11761 gtacatgttg ttattgatca atcaaaattc ccattaggtc aagcagtata ctttgatact
    11821 gaaaaagatg gaagaatgat ttttgcaatt ccacgtgaag gaaaagcgta tgtaggtact
    11881 acagatacat tctatgacaa tatcaaatct tcaccattaa ctacacaaga agacagagac
    11941 tatttaatcg atgcgattaa ttacatgttc cctagtgtta atgttacaga tgaagatatt
    12001 gaatcaacat gggcaggaat tagaccatta atttacgaag aaggcaaaga cccttctgaa
    12061 atctctcgta aggatgaaat ttgggaaggt aaatcaggtt tattaactat tgcaggtggt
    12121 aaattaacag gctatcgtca catggctcaa gacattgttg atttagtatc taaacgcttg
    12181 aaaaaagact acggtttaac atttagtcca tgtaatacaa aaggtctggc aatttcaggt
    12241 ggcgatgtag gtggtagcaa gaactttgat gcgtttgtag agcaaaaagt agatgtagct
    12301 aaaggattcg gcattgatga agatgttgca agacgtttag catctaaata tggttcaaat
    12361 gttgatgaat tgttcaacat tgcgcaaaca tctcaatacc atgatagcaa gttaccatta
    12421 gaaatttatg tagaacttgt ttatagtatt caacaagaaa tggtatacaa acctaacgat
    12481 ttcttagttc gtcgttctgg taaaatgtat ttcaatatta aagatgtatt agattataaa
    12541 gatgctgtca tcgatattat ggcagatatg cttgattact ctccagctca aattgaagca
    12601 tatactgaag aagttgagca agcaattaaa gaagcgcaac atggaaataa tcaaccagca
    12661 gttaaagaat aattaatttg tacaatcata aactggtgtc ctgttttaag ggcatcagtt
    12721 tttttatacg agatacatta gtcaattgaa actatgcatc actaaatgta tgataataat
    12781 aaaagcgaat ataaaaacgt tgttattaaa ggtgtgagat gatgactgaa aaacaattta
    12841 aattaactgt acaagataat acgaatattg aagttaaagt gaattttaca gatgtagatt
    12901 caaaaggaat tattcatata tttcatggta tggctgaaca tatggaacgt tacgataaat
    12961 tagcacatgc actttcaaag catggcttcg atgtgatacg tcataatcat cgaggacatg
    13021 gtattaatat tgatgaatca acaagagggc attacgatga tatgaaacga gttatcggtg
    13081 atgcctttga agtagcgcaa acagtgagag gcaatgttga taaaccatac attataatcg
    13141 gacattcaat gggatccgtt atagctagat tgtttgtaga aacatatccg caatatgttg
    13201 atggtctaat tttaagtggt actggtatgt attcattatg gaaaggttta ccaaccgtta
    13261 aagtgttaca actgattaca aaaatttatg gtgctgagaa acgagttgaa tgggttaacc
    13321 agttagtatc aaatagtttt aataaaaaaa tacgtccatt acgtacacaa agtgattgga
    13381 tttctagtaa tccaattgaa gtagataact ttattaaaga tccatatagt ggatttaatg
    13441 tgtcaaatca attattatat caaacagcct attatatgct acatacatca caattaaaaa
    13501 atatgaaaat gttaaatcat gccatgccta tattattagt ttcaggatat gacgattctt
    13561 taggtgatta tggtaaaggg attttaaaat tggcgaatat atatagaaaa gctggcatta
    13621 aaaatgttaa agtgaatctt tatcatcata aacgtcatga agtgttattt gaaaaagatc
    13681 atgacaaaat ttgggaagac ttgtttaaat ggttgaatca attttataaa aaataaagaa
    13741 agtggaatta aatatgaata aaaataagcc ttttattgta gtaattgtgg ggccaactgc
    13801 ttcaggtaaa acagagctta gcatagaact cgcgaagcgt atcaatggtg aaatcataag
    13861 cggtgattct atgcaagttt acaaacatat gaatattgga actgcaaaag taacacctga
    13921 agaaatggat ggtattccac atcatttaat tgatatcttg aatcctgatg atacattttc
    13981 agcatatgaa ttcaagcgat tagcagaaga tttaattact gatataacga atagaggtaa
    14041 agttccaatc atagcaggtg gaacaggctt atatattcaa tcattaatat ataattatga
    14101 attagaagat gaaacagtta cacctgcaca attatccata gttaaacaaa agttatctgc
    14161 attagaacat ttagataatc agcaactaca cgattattta gctcaatttg atgcggtttc
    14221 tgcagaaaat attcacccta acaaccgcca aagagtgttg cgcgctattg aatattattt
    14281 aaaaacaaaa aaacttttga gtaatcgcaa gaaagtgcaa caatttactg aaaattatga
    14341 tacattatta ttaggaattg aaatgtcgcg taaaacatta tattcaagaa taaataaacg
    14401 tgttgatatt atgttggatc acggattatt tagagaagtg caacaacttg ttgaacaagg
    14461 ctatgaatct tgccaaagta tgcaagctat tggatataaa gaattaatac ctgtgattaa
    14521 cggacaaatg atttatgaag atgctgtcaa tgatttaaag caacattcac gccaatatgc
    14581 aaaacgacaa atgacatggt tcaagaataa aatgagtgtt cattggttag ataaagaaaa
    14641 tatgtcactt caaatgatgt tagatgagat tacaacccag attaagtaaa aggagtccga
    14701 cagatgattg caaacgaaaa catccaagac aaagcactag agaattttaa agcaaaccaa
    14761 actgaagtaa ctgtattctt tctaaacggt ttccaaatga aaggtgttat tgaagaatac
    14821 gacaagtatg tcgtaagctt aaattctcaa ggcaaacaac acttgattta caaacatgcg
    14881 atcagcactt atacagtaga aactgaaggt caagcatcta ctgaaagtga agaataagtt
    14941 gtaatcatat gggcacgatt taatgactcg tgtacataac gtttgaagta tgataaatag
    15001 atatagaaat aaagtcatat gtgacatcaa ttaaatgatg ttcaaatgac aagatacaat
    15061 agaggaatgt ttgtaataaa aacgcttcat
//
LOCUS       DAOBBH010000046        14843 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.046, whole genome shotgun sequence.
ACCESSION   DAOBBH010000046 DAOBBH010000000
VERSION     DAOBBH010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14843
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.046"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(217..1146)
                     /locus_tag="P6L01_001073"
     CDS             complement(217..1146)
                     /locus_tag="P6L01_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDP3030154.1"
                     /translation="MTNSSKSFTKFMAASAVFTMGFLSVPTAGAEQTNQIANKPQAIQ
                     WHTNLTNERFTTIAHRGASGYAPEHTFQAYDKSHNELKASYIEIDLQRTKDGHLVAMH
                     DETVNRTTNGHGKVEDYTLDELKQLDAGSWFNKKYPKYARASYKNAKVPTLDEILERY
                     GPNANYYIETKSPDVYPGMEEQLLASLKKHHLLNNNKLKNGHVMIQSFSDESLKKIHR
                     QNKHVPLVKLVDKGELQQFNDQRLKEIRSYAIGLGPDYTDLTEQNTHHLKDLGFIVHP
                     YTVNEKADMLRLNKYGVDGVFTNFADKYKEVIK"
     gene            complement(1388..2767)
                     /gene="argH"
                     /locus_tag="P6L01_001074"
     CDS             complement(1388..2767)
                     /gene="argH"
                     /locus_tag="P6L01_001074"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDP3030155.1"
                     /translation="MSNKAWGGRFEVQPEEWVDDFNASITFDQTLIDQDIEGSIAHAT
                     MLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGG
                     KLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPGYTHLQR
                     AQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLN
                     FGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFST
                     GSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVH
                     TIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKI
                     VLECIQQGHYLLDVPLATYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQL
                     DVAKQLLSQ"
     gene            complement(2757..3962)
                     /locus_tag="P6L01_001075"
     CDS             complement(2757..3962)
                     /locus_tag="P6L01_001075"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDP3030156.1"
                     /translation="MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDI
                     VYKKALDMGAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVE
                     IAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPVREWAWSREEEIDYAIKH
                     NIPVSINHDSPYSIDQNLWGRANECGILEDPYAAPPEDAFDLTNALEETPDTADEIIL
                     TFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAE
                     VILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVS
                     GDVRIKLFKGNAIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNAM
                     LHGGYSNEQ"
     gene            4313..5644
                     /locus_tag="P6L01_001076"
     CDS             4313..5644
                     /locus_tag="P6L01_001076"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDP3030157.1"
                     /translation="MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDF
                     LGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNS
                     NEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEER
                     YGKEEAQKRIFATTDKEKGALKQLATNEGYETFIVPDDVGGRYSVLTAVGLLPIATAG
                     INIEAMMIGAAKAREELSSDKLEDNIAYQYATIRNILYAKGYTTEMLINYEPSMQYFN
                     EWWKQLFGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDIT
                     IEKDSDDLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVV
                     YFFELACAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEERL"
     gene            5969..6544
                     /locus_tag="P6L01_001077"
     CDS             5969..6544
                     /locus_tag="P6L01_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDP3030158.1"
                     /translation="MSFHQVEEWFEIFRQFGYLPGFILLYIRAIIPVFPLALYILINI
                     QAYGPILGILISWLGLISGTFTVYLICKRLVNTERMQRIKQRTAVQRLISFIDRQGLI
                     PLFILLCFPFTPNTLINFVASLSHIRPKYYFIVLASSKLVSTIILGYLGKEITTILTH
                     PLRGILMLVVLVVFWIVGKKLEQHFMGSKKE"
     gene            6549..7073
                     /gene="lepB"
                     /locus_tag="P6L01_001078"
     CDS             6549..7073
                     /gene="lepB"
                     /locus_tag="P6L01_001078"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDP3030159.1"
                     /translation="MKKVVKYLISLILAIIIVLFVQTFVIVGHVIPNNDMSPTLNKGD
                     RVIVNKIKVTFNQLNNGDIITYRRGNEIYTSRIIAKPGQSMAFRQGQLYRDDRPVDAS
                     YAKNRKIKDFSLRNFKELDGDIIPPNNFVVLNDHDNNQHDSRQFGLIDKKDIIGNISL
                     RYYPFSKWTIQFKS"
     gene            7089..7664
                     /gene="lepB"
                     /locus_tag="P6L01_001079"
     CDS             7089..7664
                     /gene="lepB"
                     /locus_tag="P6L01_001079"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDP3030160.1"
                     /translation="MKKELLEWIISIAVAFVILFIVGKFIVTPYTIKGESMDPTLKDG
                     ERVAVNIIGYKTGGLEKGNVVVFHANKNDDYVKRVIGVPGDKVEYKNDTLYVNGKKQD
                     EPYLNYNLKHKQGDYITGTFQVKDLPNANPKSNVIPKGKYLVLGDNREVSKDSRAFGL
                     IDEDQIVGKVSFRFWPFSEFKHNFNPENTKN"
     gene            7824..11297
                     /gene="addB"
                     /locus_tag="P6L01_001080"
     CDS             7824..11297
                     /gene="addB"
                     /locus_tag="P6L01_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HDP3030161.1"
                     /translation="MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQST
                     FQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQE
                     QQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLEHFIADKNMQTRTKNKLEDI
                     ALIYREFEQRIQNEFITGEDSLQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKG
                     LIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEIANELNISIERQYFNQLYRFNN
                     QDLKHLEQEFDVLQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIA
                     ILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQVNPMLR
                     LLKTDVLTASYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKAHKLTE
                     DERNTFEQVVKLKKDVIDKILHFEKQMSQAETVKDFATAFYESMEYFELPNQLMTERD
                     ELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMI
                     PQTLDQVSIGTMDLAKVDNKQHVYLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVE
                     LSPTSDILQMDEAFVCYVAMTRAKGDVTFSYSLMGSSGDDKEISPFLNQIQSLFNQLE
                     ITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLDDEIVADSWLDAYQVIRDSDHLNQ
                     GLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNE
                     RTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEEILPKVQFNLL
                     NSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLT
                     TTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYM
                     DIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWSDIDEDKLEQDLIKKFKLSGLV
                     NADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFI
                     ETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQ
                     NININDEFGGE"
     gene            11301..>14843
                     /gene="addA"
                     /locus_tag="P6L01_001081"
     CDS             11301..>14843
                     /gene="addA"
                     /locus_tag="P6L01_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDP3030162.1"
                     /translation="MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVER
                     IIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRIQEASIADPANAHLKNQRIKIHQ
                     AQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
                     IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPTNWLDQLVTPYEEEAQQAQLIQLLT
                     DLSKVFITAAYDALNKAYDLFSMMDGVDKHLAVIEDERRLMGRVLEGGFIDIPYLTDH
                     EFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKNDYFSREADDLKADMQ
                     QLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYR
                     QHFQEILVDEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIE
                     KYQRFTIDGDGTGRRIDLSQNFRSRKEVLSTTNYIFKHMMDEQVGEVKYDEAAQLYYG
                     APYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMKTGSYRS
                     ATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPL
                     QDIYLVGLMRSVIYQFKEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMF
                     LSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIGGRGRRANLYGLFNKAIEFEN
                     SSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLS
                     KDFNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYKAVAEKELVSEEMRLVY
                     VALTRAKEQLYLIGRVKNDKSLLELEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQ
                     SASIPDDLKFEKDIAQVEDSSRPNVNISIIYFEDVSTETILDNNEYRSVNQLETMQNG
                     NEDVKAQIKHQLDYQYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFST
                     YERPKFLSEQGKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADA
                     KKDIRMDEIMTFINSELYSIIAEAEQVYRELPFVVNQALVDQLPQGDEDVSIIQGMID
                     LIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLK"
BASE COUNT         5145 a         2224 c         2617 g         4857 t
ORIGIN      
        1 tagtcagggg ccccaacaca gagaatttcg aaaagaaatt ctacagacaa tgcaagttgg
       61 cgaggcccca acactgagaa tttctaatta aacgctcaac tatatatagt tgatcaaaaa
      121 gacagcagta ggataatttc aatttggaaa tatcttactg ctgtttttta tagtaatagc
      181 tatatttttg tatttatgtt ttctagttta acaatactac ttaatgactt ctttatattt
      241 atcagcgaaa tttgtaaaga caccatcaac gccatattta tttaatcgta acatatcagc
      301 tttttcattc actgtataag gatgtactat aaatcctaag tcttttaaat gatgggtatt
      361 ttgttcagtt aaatctgtat aatcaggacc taatccaatc gcataagagc gtatctcttt
      421 taagcgttgg tcgttaaatt gttgtagttc acctttatca actaatttta ctaatggcac
      481 atgcttattt tgacgatgaa ttttctttaa actttcgtca gaaaatgatt gaatcattac
      541 atgtccattt tttaatttat tgttatttaa aaggtgatgc tttttcaatg aagctaataa
      601 ttgttcttcc attcctgggt atacatcagg tgactttgtt tcaatataat agtttgcatt
      661 cgggccataa cgttctaaaa tttcatctaa agtgggtact ttagcatttt tataacttgc
      721 tcttgcgtat tttggatatt ttttattaaa ccaacttcct gcatctaact gttttaattc
      781 atcaagggta taatcctcaa ctttaccgtg tccatttgtt gtacggttaa cagtttcatc
      841 atgcatagca actaaatgtc catctttggt acgttgtaaa tcaatttcga tataagatgc
      901 ttttaactca ttatgactct tatcatatgc ttgaaacgta tgctctggtg catagccact
      961 tgcgccacga tgtgcgatag tagtgaatcg ctcattcgtt aaatttgtat gccattgaat
     1021 agcctgaggt ttatttgcaa tttgatttgt ttgttcagcg ccagcagtag gtactgataa
     1081 aaatcccata gtaaaaacag cagaagcagc cataaattta gtgaagcttt tcgaagagtt
     1141 agtcatagtc tttatccttt catctcttta tttgtagtct aacaatttta aaatactaca
     1201 aaaacctgct tgtgaaaacg ctttacacat ttcttttaca atttataaca tttctgtaat
     1261 ataaatttaa aagaattatc aaacatttta ggaatcttaa aaatgtgatg ttcaacaaga
     1321 cgaagagagc attaaaatac ccacaatgta attttaatag acattgtggg taggtagatt
     1381 aacgtattta ttgtgatagt aattgtttag caacatcaag ttgttgtttg accgatgatt
     1441 gccccgttga cccgtaactt tgacgtcgtt tcaagcaatt ttcaggctgc aaataatcgt
     1501 aaatatcggc atcgatacta gaatgatgtt gttgatatgt tgctaaagga acatctaata
     1561 aataatgacc ttgttgtata cattctaaga cgatttttcc tacaatttca tgtgcagttc
     1621 taaatggaat atttttagtt actaaataat ctgctagttc cgttgcattt gaaaaatctt
     1681 ctttaacagt ttgattgagt cgttctttat taattgtcat cgtttgaatc ataccttcga
     1741 aaatacgtaa agaaccttta attgtatgga cagcatcgaa taaaccttct ttatcttcct
     1801 gcatatcttt gttatatgct agaggtaatc cttttaaagt cataagcatg ctcattaaat
     1861 gaccagtcgt tcgaccaact ttacctctaa ttaattctgc catatcagga tttttctttt
     1921 gtggcataat agatgagcca gttgaaaatg catctgataa tgtaatgaat ttagcttcgt
     1981 ctgtggacca gaaaataatt tcctctgcaa agcgtgataa gtgaaccatc gttaaagaaa
     2041 tattatgcaa tgtttcaata atatagtctc tgtcactaac agcatctagg ctattctcat
     2101 agagactgcc aaagttcaac aatgctgttg tctcgtgtct atcgataggg tatgtggtac
     2161 cacttaaggc tgctgcacct aaaggattaa tatcgattcg ttttaaacta tcttcaaatc
     2221 gttgttggtc tcgttgtaac atccaaaaat aagtcataat atgatgtgca aatgaaattg
     2281 gctgtgcacg ctgtaaatga gtataaccag gcataattgt atcaacatta ttggaagcga
     2341 tgtctacaat tacactttgt aacgacttaa ttaatgcgat gatatcttgc acttgtttct
     2401 tagtgtacaa gtgcatatct gttgcaactt gatcgtttct actgcgtcca gtatgcaact
     2461 taccaccagc atcaccaata cgtttaatta attcatgttc aatatttaaa tgaatatctt
     2521 ctaatgatgc actaaattga atttgatctt gatgataatc atgttgaata gattttagtc
     2581 cttgtataat ttgttcgctg tcttgttgac taataatgcc ttgattcgca agcatagttg
     2641 catgcgcaat gctgccttcg atatcttgat ctatgagcgt ttggtcaaaa gtaatggatg
     2701 cgttaaagtc gtcaacccac tcttcaggtt gtacttcaaa tctaccgccc caagctttat
     2761 tgctcattgc tatagcctcc atgcaacatc gcatttactt gagtaggtaa accatagata
     2821 tcgataaagc caacagcagc gtcttgatta aatgcatctt cttttgtata agttgctaat
     2881 ttttcatcat ataatgtgta aggtgatttt ctaccattca cgatggcatt acctttgaat
     2941 aatttaattc tgacatcacc acttacgtat tgctgagtac tatcaataaa taatttcaag
     3001 ctatcagtta aaggtgagaa ccaaagtcca ttgtatagtt gttcagcaaa ttgcttctca
     3061 atgattggtt taaagtgtgc gacatcttta gttaacgtaa tcgtttctaa tgctttatgc
     3121 gcttttaaaa taacttctgc agcaggtgcc tcataaattt ctcttgattt gatacctaca
     3181 agtctatttt ctacatggtc aattcttccg ataccatgct taccagctaa tgcattcaac
     3241 gttaaaatta aatcgtctaa ttcatatgtt ttgccatcaa tttgaactgg gatgccttta
     3301 tcaaacgtta aaatgatttc atcagcagta tctggtgttt cttctaaagc atttgttaga
     3361 tcgaacgcat cctctggtgg cgcagcataa ggatcttcta aaataccaca ttcattcgct
     3421 ctgccccata gattttgatc gatagaataa ggtgaatcat ggttgattga tacagggata
     3481 ttatgtttaa ttgcataatc gatttcttct tcacgactcc atgcccactc acgtacaggt
     3541 gcgaatgctt tcaatgatgg gtttaatgct ttaatggcaa cttcgaaacg tacttggtca
     3601 ttccctttac cagtacaacc atgtgcaata cctactgaat ttgttttctc agcaatctct
     3661 actaattttt tagcgattaa tggtcttgat aaagctgaaa ctaatggata tgcattttca
     3721 tacattaaat ttcctttgat tgcataactt acatactcat cactaaattc ttttgttgca
     3781 tcaataatat gacattcaac tgctcccata tctaaagctt ttttataaac gatgtctaaa
     3841 tctttacctt caccaacatc taggcaacaa gctacaacgt cgtatccttt gtcgataagc
     3901 cattgaacgg ccacacttgt atctagtcct cctgaatatg ctaaaacaat tttctctttc
     3961 ataaaattca cctcattgta gttatctgaa tatttcgtat gaacacatga tagcaaactt
     4021 ttaatgaaaa taaaaccatt attttacata tttattcata aaatgcgata gagatagtta
     4081 gagttgtaag tataaatgct tatttatcaa tgtttaaaag tattttaaaa aacatgcaaa
     4141 ttgtataaaa aatgtttgag tgtttgacgc cagtttgaaa tgtttgttga aaaataggtg
     4201 tgaaacgtca cataaatatc aattttattg attaagtaca aacttgtgaa ttgaatcgca
     4261 aacgtaaact tagtaaaatt aagatattaa atgaaacagg aggcttttat agatgactca
     4321 tattcaatta gattttagta aaacgttaga atttttcggt gaacacgaat taaaacaaca
     4381 acaagaaatt gttaaatcaa ttcacaaaac aattcatgaa ggtactggtg caggtagtga
     4441 cttcttaggc tgggttgatt taccagttga ttacgacaaa gaagaatttt caagaattgt
     4501 tgaagcatca aaacgcatta aagaaaattc tgatgtttta gtagtcatcg gtattggtgg
     4561 ttcttactta ggtgcacgtg cagcaatcga aatgttaacg tcatcattta gaaacagcaa
     4621 tgaataccct gaaattgtat ttgttggtaa tcacttatca tcaacatata cgaaagagtt
     4681 agttgattat ttagcagaca aagatttctc tgtaaacgtt atttctaaat ctggtacaac
     4741 tacagaacca gcagttgcat ttagattgtt caaacaatta gttgaagaaa gatacggtaa
     4801 agaagaagca caaaaacgta tatttgcaac aacggataaa gaaaaaggtg ctttaaaaca
     4861 gttggctaca aacgaaggtt atgaaacgtt tatcgtacct gatgatgtag gtggaagata
     4921 ttctgtttta acagcagtag gattattacc aattgcaaca gctggaatta acatcgaagc
     4981 tatgatgatt ggtgctgcaa aagcacgtga agaattatct tcagataaat tagaagacaa
     5041 cattgcatac caatatgcga caattcgaaa cattttatat gcaaaaggtt atacaacaga
     5101 aatgttgatt aactatgaac catctatgca atactttaat gaatggtgga aacaattatt
     5161 tggtgaatca gaaggtaaag acttcaaagg tatctatcct tcaagtgcca actacacaac
     5221 tgatttacat tctttaggtc aatatgtaca agaaggccgt cgtttcttat tcgaaacagt
     5281 ggtaaaagta aatcatccta aatatgatat tactattgaa aaagatagtg atgatctaga
     5341 cggattaaat tatttggctg gtaaaacaat cgacgaagtt aacacaaaag cattcgaagg
     5401 tacattatta gcgcatactg atggtggtgt tcctaacatg gtagtgaaca ttccacaatt
     5461 agatgaagaa actttcggtt atgtcgtata cttcttcgaa cttgcttgtg caatgagtgg
     5521 ataccaatta ggtgtaaatc catttaacca acctggtgta gaagcatata aacaaaacat
     5581 gttcgcatta ttaggtaaac ctggttttga agacttgaaa aaagaattag aagagcgttt
     5641 ataaaataca ttacttcaat gattagtgaa gtttgaaaag atagaactag acgttaacta
     5701 tttaaagcat attttcgagg ttgtcattac aaatgtaaaa atgtaatgac aacctcgttt
     5761 ttatttatat gcaagaacta ggttactagc taatgtgaca agatgttaag agaaaattaa
     5821 agaaaaaata acatctgtca tacaataata ttgttatact actagagact gatttattag
     5881 catgattaca tgttaatgtt tctttactta gtaattaact ttataatgta agaataatta
     5941 tcttcaacca aagaaaggga ttgatgattt gtcgtttcat caagtagaag aatggtttga
     6001 gatatttcga cagtttggtt atttacctgg atttatattg ttatatatta gagcgataat
     6061 tccagtattt cctttagcac tctatatttt aattaacatt caagcttatg gacctatttt
     6121 aggtatattg attagttggc ttggattaat ttctggaaca tttacagtct atttgatctg
     6181 taaacgattg gtgaacactg agaggatgca gcgaattaaa caacgtactg ctgttcaacg
     6241 cttgattagt tttattgatc gccaaggatt aatcccattg tttattttac tttgttttcc
     6301 ttttacgcca aatacattaa taaattttgt agcgagtcta tctcatatta gacctaaata
     6361 ttatttcatt gttttggcat catcaaagtt agtttcaaca attattttag gttatttagg
     6421 taaggaaatt actacaattt taacgcatcc tttaagaggg atattaatgt tagttgtgtt
     6481 ggttgtattt tggattgttg gaaaaaagtt agaacagcat tttatgggat cgaaaaagga
     6541 gtgacatcgt gaaaaaagtt gtaaaatatt tgatttcatt gatacttgct attatcattg
     6601 tactgttcgt acaaactttt gtaatagttg gtcatgtcat tccgaataat gatatgtcac
     6661 caacccttaa caaaggggat cgtgttattg taaataaaat taaagttaca tttaatcaat
     6721 tgaataatgg tgatatcatt acatataggc gtggtaacga gatatatact agtcgaatta
     6781 ttgccaaacc tggtcaatca atggcgtttc gtcagggaca attataccgt gatgaccgac
     6841 cggttgacgc atcttatgcc aagaacagaa aaattaaaga ttttagtttg cgcaatttta
     6901 aagaattaga tggagatatt ataccgccta acaattttgt tgtgctaaat gatcatgata
     6961 acaatcagca tgattctaga caatttggtt taattgataa aaaggatatt attggtaata
     7021 taagtttgag atattatcct ttttcaaaat ggacgattca gttcaaatct taaaaagagg
     7081 tgtcaaaatt gaaaaaagaa ttattggaat ggattatttc aattgcagtc gcttttgtca
     7141 ttttatttat agtaggtaaa tttattgtta caccatatac aattaaaggt gaatcaatgg
     7201 atccaacttt gaaagatggc gagcgagtag ctgtaaacat tattggatat aaaacaggtg
     7261 gtttggaaaa aggtaatgta gttgtcttcc atgcaaacaa aaatgatgac tatgttaaac
     7321 gtgtcatcgg tgttcctggt gataaagtag aatataaaaa tgatacatta tatgtcaatg
     7381 gtaaaaaaca agatgaacca tatttaaact ataatttaaa acataaacaa ggtgattaca
     7441 ttactgggac tttccaagtt aaagatttac cgaatgcgaa tcctaaatca aatgtcattc
     7501 caaaaggtaa atatttagtt cttggagata atcgtgaagt aagtaaagat agccgtgcgt
     7561 ttggcctcat tgatgaagac caaattgttg gtaaagtttc atttagattc tggccattta
     7621 gtgaatttaa acataatttc aatcctgaaa atactaaaaa ttaatatgaa acaaatacaa
     7681 catcgtttgt cggttttaat actgataaac gatgttttat tttgttagta ccacaataaa
     7741 agctaagttc gaaatgaact tataataaat caatcacaat cactttgtgt taaaatatgt
     7801 gtcaaaggaa gtgagggttt gtcatgacat tacatgctta tttaggtaga gcgggaacag
     7861 gtaagtctac gaaaatgttg accgaaataa aacaaaaaat gaaagcagat ccgcttggag
     7921 atccaatcat tttaattgcg ccaactcaaa gtacatttca attagaacaa gcctttgtca
     7981 atgatccgga attaaatggt agtttaagaa cagaagtgtt gcattttgaa cgattaagtc
     8041 atcgtatttt ccaagaagtt ggtagttata gcgaacaaaa gttatctaaa gctgcaacgg
     8101 aaatgatgat ttataacatt gttcaagaac aacaaaagta tttaaaactt tatcaatcac
     8161 aagcaaaata ttatgggttt agtgaaaaat taacagaaca aattcaagat tttaaaaaat
     8221 atgcagtaac gcctgaacat ttagaacact ttattgctga taaaaatatg caaactcgaa
     8281 ctaaaaataa gttagaggat attgctttaa tataccgtga gttcgaacaa cgcattcaaa
     8341 acgagtttat tactggtgag gattcattac aatattttat tgattgtatg ccgaaatcag
     8401 agtggctaaa acgtgctgat atatatattg atggttttca caacttttca acgattgagt
     8461 atttaataat caaaggatta attaaatatg cgaagagtgt cacaattata ttgacgacag
     8521 atggtaacca cgatcaattt agtttattta gaaaaccatc ggaagtgtta cgacatattg
     8581 aagaaatagc aaatgaactc aatatttcta ttgaacgtca atatttcaac caattatatc
     8641 gcttcaataa tcaagattta aagcatcttg aacaagaatt tgatgtactt caaatcaatc
     8701 gagtggcatg tcaaggtcat atcaatattt tagaatctgc gactatgaga gaggaaataa
     8761 atgaaattgc gcgacgtatc atcgttgata ttcgtgataa gcaattacga tatcaagata
     8821 ttgcaatttt atatcgtgac gagtcttatg cttatttatt tgattccata ttaccgcttt
     8881 ataatattcc ttataacatt gatacaaagc gttcgatgac acatcatccg gtcatggaaa
     8941 tgattcgttc attgattgaa gttattcaat ctaattggca agtgaatcca atgctacgct
     9001 tattgaagac tgatgtgtta acggcatcat atctaaaaag tgcatactta gttgatttac
     9061 ttgaaaattt tgtacttgaa cgtggtatat acggtaaacg ttggttagat gatgagctat
     9121 ttaatgtcga acattttagc aaaatggggc gtaaagcgca taaactgacc gaagatgaac
     9181 gtaacacatt tgaacaagtc gttaagttaa agaaagatgt cattgataaa attttacatt
     9241 ttgaaaagca aatgtcacaa gcggaaactg taaaagactt tgcaactgct ttttatgaaa
     9301 gtatggaata tttcgaactg ccaaatcaat tgatgacaga gcgagatgaa cttgatttaa
     9361 atggtaatca tgaaaaggcg gaggaaattg atcaaatatg gaatggctta attcaaatcc
     9421 ttgacgactt agttctagta tttggagatg aaccaatgtc gatggaacgt ttcttagaag
     9481 tatttgatat tggtttagaa caattagaat ttgtcatgat tccacaaaca ttagatcaag
     9541 ttagtattgg tacgatggat ttggctaaag tcgacaataa gcaacatgtt tacttagttg
     9601 gaatgaacga cggcaccatg ccacaaccag taactgcatc aagtttaatt actgatgaag
     9661 aaaagaaata ttttgaacaa caagcaaatg tagagttgag tcctacatca gatattttac
     9721 agatggatga agcatttgtt tgctatgttg ctatgactag agctaaggga gatgttacat
     9781 tttcttacag tctaatggga tcaagtggtg atgataagga gatcagccca tttttaaatc
     9841 aaattcaatc attgttcaac caattggaaa ttactaacat tcctcaatac catgaagtta
     9901 acccattgtc actaatgcaa catgctaagc aaaccaaaat tacattattt gaagcattgc
     9961 gtgcttggtt agatgatgaa attgtggctg atagttggtt agatgcttat caagtaatta
    10021 gagatagcga tcatttaaat caaggtttag attatttaat gtcagcatta acgtttgaca
    10081 atgaaactgt aaaattaggt gaaacgttgt ctaaagattt atatggtaag gaaatcaatg
    10141 ccagtgtatc tcgttttgaa ggttatcaac aatgcccatt taaacactat gcttcacatg
    10201 gtctgaaact aaatgaacga acgaaatatg aacttcaaaa ctttgattta ggtgatattt
    10261 tccattccgt tttaaaatat atatctgaac gtattaatgg cgattttaaa caattagacc
    10321 tgaaaaaaat aagacaatta acgaatgaag cattggaaga aattttacct aaagttcagt
    10381 ttaatttatt aaattcttca gcttactatc gttatttatc aagacgcatt ggcgctattg
    10441 tagaaacaac actaagcgca ttaaaatatc aaggcacgta ttcaaagttt atgccaaaac
    10501 attttgagac aagttttaga aggaaaccaa gaacaaatga cgaattaatt gcacaaacat
    10561 taacgacaac tcaaggtatt ccaattaata ttagagggca aattgaccgt atcgatacgt
    10621 atacaaagaa tgatacaagt tttgttaata tcattgacta taaatcctct gaaggtagtg
    10681 cgacacttga tttaacgaaa gtatattatg gtatgcaaat gcaaatgatg acatacatgg
    10741 atatcgtttt acaaaataaa caacgccttg gattaacaga tattgtgaaa ccaggtggat
    10801 tattatactt ccatgtacat gaacctagaa ttaaatttaa atcatggtct gatattgatg
    10861 aagataaact agaacaagat ttaattaaaa agtttaagct gagtggttta gtgaatgcag
    10921 accaaactgt tattgatgca ttggatattc gtttagaacc taaattcact tcagatattg
    10981 taccagttgg tttgaataaa gatggctctt tgagtaaacg aggcagccaa gtggcagatg
    11041 aagcaacaat ttataaattc attcagcata acaaagagaa ttttatagaa acagcttcaa
    11101 atattatgga tggacatact gaagttgcac cattaaagta caaacaaaaa ttgccatgtg
    11161 ctttttgtag ttatcaatcg gtatgtcatg tagatggcat gattgatagt aagcgatatc
    11221 gaactgtaga tgaaacaata aatccaattg aagcaattca aaatattaac attaatgatg
    11281 aatttggggg tgagtaatag atgacaattc cagagaaacc acaaggcgtg atttggactg
    11341 acgcgcaatg gcaaagtatt tacgcaactg gacaagatgt acttgttgca gccgcggcag
    11401 gttcaggtaa aacagctgta ctagttgagc gtattatcca aaagatttta cgtgatggca
    11461 ttgatgtcga tcgactttta gtcgtaacgt ttacaaactt aagcgcacgt gaaatgaagc
    11521 atcgtgtaga ccaacgtatt caagaggcat cgattgctga tcctgcaaat gcacacttga
    11581 aaaaccaacg catcaaaatt catcaagcac aaatatctac acttcatagt ttttgcttga
    11641 aattaattca acagcattat gatgtattaa atattgaccc gaactttaga acaagcagtg
    11701 aagctgaaaa tattttatta ttagaacaaa cgatagatga ggtcatagaa caacattacg
    11761 atatccttga tcctgctttt attgaattaa cagaacaatt gtcttcagat agaagtgatg
    11821 atcagtttcg aatgattatt aaacaattgt atttctttag cgttgcaaat ccaaatccta
    11881 caaattggtt ggatcaattg gtgacaccat acgaagaaga agcacaacaa gcgcaactta
    11941 ttcaactact aacagactta tctaaagtat ttatcacagc tgcctatgat gctttaaata
    12001 aggcgtatga tttgtttagt atgatggatg gcgtcgataa acatttagct gttatagaag
    12061 atgaacgacg tttaatgggg cgtgttttag aaggtggttt tattgatata ccttatttaa
    12121 ctgatcacga atttggcgcg cgtttgccta atgtaacagc gaaaattaaa gaagcaaatg
    12181 aaatgatggt cgatgcctta gaagatgcta aacttcagta taaaaaatat aaatcattaa
    12241 ttgataaagt gaaaaatgat tacttttcaa gagaagctga tgatttgaaa gctgatatgc
    12301 aacaattggc gccacgagta aagtaccttg cgcgtattgt gaaagatgtt atgtcagaat
    12361 tcaatcgaaa aaagcgtagc aaaaatattc tggatttttc tgattatgaa cattttgcat
    12421 tacaaatttt aactaatgag gatggttcgc cttcagaaat tgccgaatca taccgtcaac
    12481 actttcaaga aatattggtc gatgagtatc aagatacgaa ccgggttcaa gagaaaatac
    12541 tatcttgcat caaaacgggt gatgaacata atggtaattt atttatggtt ggagatgtta
    12601 agcaatccat ttataaattt agacaagctg atccaagttt atttattgaa aagtatcaac
    12661 gctttactat agatggagat ggcactggac gtcgaattga tttgtcgcaa aacttccgtt
    12721 ctcgaaaaga agtactgtca acgactaact atatattcaa acatatgatg gatgaacaag
    12781 tcggtgaagt aaaatatgat gaagcggcac agttgtatta tggtgcacca tatgatgaat
    12841 cggaccatcc agtaaactta aaagtgcttg ttgaagcgga tcaagaacat agtgatttaa
    12901 ctggtagtga acaagaagcg cattttatag tagaacaagt taaagatatc ttagaacatc
    12961 aaaaagttta tgatatgaaa acaggaagct atagaagtgc gacatacaaa gatatcgtta
    13021 ttctagaacg cagctttgga caagctcgca atttacaaca agcctttaaa aatgaagata
    13081 ttccattcca tgtgaatagt cgtgaaggtt actttgaaca aacagaagtc cgcttagtat
    13141 tatcattttt aagagcgata gataatccat tacaagatat ttatttagtt gggttaatgc
    13201 gctccgttat atatcagttc aaagaagacg aattagctca aattagaata ttgagtccaa
    13261 atgatgacta cttctatcaa tcgattgtaa attacattaa tgacgaagca gcagatgcta
    13321 ttttagttga taaattaaaa atgtttttat cagatattca aagttaccaa caatatagta
    13381 aagatcatcc ggtgtatcag ttaattgata aattttataa tgatcattat gttattcaat
    13441 actttagtgg acttattggt ggacgtggac gacgtgcaaa cctttatggt ttatttaata
    13501 aagctatcga gtttgagaat tcaagtttta gaggtttata tcaatttatt cgttttatcg
    13561 atgaattgat tgaacgaggc aaagattttg gtgaggaaaa tgtagttggt ccaaacgata
    13621 atgttgttag aatgatgaca attcatagta gtaaaggtct agagtttcca tttgtcattt
    13681 attctggatt gtcaaaagat tttaataaac gtgatttgaa acaaccagtt attttaaatc
    13741 agcaatttgg tctcggaatg gattattttg atgtggataa agaaatggca tttccatctt
    13801 tagcttcggt tgcatataaa gctgttgccg aaaaagaact tgtgtcagaa gaaatgcgat
    13861 tagtctatgt agcattaaca agagcgaaag aacaacttta tttaattggt agagtgaaaa
    13921 atgataaatc gttactagaa ctagagcaat tgtctatttc tggtgagcac attgctgtca
    13981 atgaacgatt aacttcacca aatccgttcc atcttattta tagtatttta tctaaacatc
    14041 aatctgcgtc aattccagat gatttaaaat ttgaaaaaga tatagcacaa gttgaagata
    14101 gtagtcgtcc gaatgtaaat atttcaatta tatactttga agatgtgtct acagaaacca
    14161 ttttagataa taatgaatat cgttcggtta atcaattaga aactatgcaa aatggtaatg
    14221 aggatgttaa agcacaaatt aaacaccaac ttgattatca atatccatat gtaaatgata
    14281 ctaaaaagcc atcaaaacaa tctgtttctg aattgaaaag gcaatatgaa acagaagaaa
    14341 gtggcacaag ttacgaacga gtaagacaat atcgtatcgg tttttcaacg tatgaacgac
    14401 ctaaatttct aagtgaacaa ggtaaacgaa aagcgaatga aattggtacg ttaatgcata
    14461 cagtgatgca acatttacca ttcaaaaaag aacgcatatc tgaagttgag ttacatcagt
    14521 atatcgatgg attaatcgat aaacatatta tcgaagcaga tgcgaaaaaa gatatccgta
    14581 tggatgaaat aatgacattt atcaatagtg agttatattc gattattgct gaagcagagc
    14641 aagtttatcg tgaattaccg tttgtagtta accaagcatt agttgaccaa ttgccacaag
    14701 gagacgaaga cgtctcaatt attcaaggta tgattgactt aatctttgtt aaagatggtg
    14761 tgcattattt tgtagactat aaaaccgatg catttaatcg tcgccgtggg atgacagatg
    14821 aagaaattgg tacacaatta aaa
//
LOCUS       DAOBBH010000047        14738 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.047, whole genome shotgun sequence.
ACCESSION   DAOBBH010000047 DAOBBH010000000
VERSION     DAOBBH010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14738
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.047"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(92..259)
                     /locus_tag="P6L01_001082"
     CDS             complement(92..259)
                     /locus_tag="P6L01_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030163.1"
                     /translation="MKRIVILENVIVDNPIWLNHVDYLVKSVLLKSIKLRFQEVRKFE
                     ILVKIMNRIHI"
     gene            complement(387..890)
                     /locus_tag="P6L01_001083"
     CDS             complement(387..890)
                     /locus_tag="P6L01_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDP3030164.1"
                     /translation="MSKWIIVSDNHTESGVLYQIYEMHPDADVYLHLGDSEFAYDDTE
                     LSLFNRVKGNCDFYPEFENEAVAKYNDVKAFYTHGHLYQVNRTRDLLAEKGLELGCLF
                     AFYGHTHVAKYEYINGVHVINPGSISQSRSSMEETYAEVIIDDQTLHGTINFKNRHHE
                     TISHTTF"
     gene            complement(883..1470)
                     /locus_tag="P6L01_001084"
     CDS             complement(883..1470)
                     /locus_tag="P6L01_001084"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HDP3030165.1"
                     /translation="MKEIVIASNNQGKINDFKVIFPDYHVIGISELIPDFDVEETGST
                     FEENAILKSEAAAKALNKTVIADDSGLEVFALNGEPGIYSARYAGENKSDEANIEKLL
                     NKLGNTTDRRAQFVCVISMSGPDMETKVFKGTVSGEIADGKYGENGFGYDPIFYVPKL
                     DKTMAQLSKEQKGQISHRRNAINLLQAFLEGEKNV"
     gene            complement(1482..2282)
                     /gene="racE"
                     /locus_tag="P6L01_001085"
     CDS             complement(1482..2282)
                     /gene="racE"
                     /locus_tag="P6L01_001085"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDP3030166.1"
                     /translation="MNKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPR
                     PGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLSIPVIGVIEPGARTA
                     IMTTRNQNVLVLGTEGTIKSEAYRTHIKRINPHVEVHGVACPGFVPLVEQMRYSDPTI
                     TSIVIHQTLKRWRNSESDTVILGCTHYPLLYKPIYDYFGGKKTVISSGLETAREVSAL
                     LTFSNEHASYTEHPDHRFFATGDPTHITNIIKEWLNLSVNVERISVND"
     gene            complement(2518..3333)
                     /gene="sdhB"
                     /locus_tag="P6L01_001086"
     CDS             complement(2518..3333)
                     /gene="sdhB"
                     /locus_tag="P6L01_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HDP3030167.1"
                     /translation="MTEQSVKNTPQHETQSKPKQKTVKLIIKRQDTSDSKPYEETFEI
                     PYRENLNVIACLMEIRRNPVNIKGEKTTPVVWDMNCLEEVCGACSMVINGRARQSCSA
                     IVDQLEQPIRLEPMNTFPVIRDLQVDRSRMFDNLKRMKAWIPIDGTYDLGPGPRMPEK
                     KRQTAYELSKCMTCGVCLEVCPNVTENNKFVGAQAISQVRLFNLHPTGSMTKDERLNA
                     LMGTGGLQQCGNSQNCVNACPKGIPLTTSIAAMNRETTFHMFKSFFGSDHEVE"
     gene            complement(3333..5099)
                     /gene="sdhA"
                     /locus_tag="P6L01_001087"
     CDS             complement(3333..5099)
                     /gene="sdhA"
                     /locus_tag="P6L01_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HDP3030168.1"
                     /translation="MAEKHLIVVGGGLAGLMSTIKAAEKGAHVDLFSVVPVKRSHSVC
                     AQGGINGAVNTKGEGDSPWIHFDDTVYGGDFLANQPPVKAMTEAAPKIIHLLDRMGVM
                     FNRTNEGLLDFRRFGGTLHHRTAYAGATTGQQLLYALDEQVRAYEVDGLVTKYEGWEF
                     LGIVKGDDDSARGIVAQNMTTAEIETFGSDAVIMATGGPGIIFGKTTNSMINTGSAAS
                     IVYQQGAIYANGEFIQIHPTAIPGDDKLRLMSESARGEGGRIWTYKDGKPWYFLEEKY
                     PDYGNLVPRDIATREIFDVCINQKLGINGENMVYLDLSHKDPHELDVKLGGIIEIYEK
                     FTGDDPRKVPMKIFPAVHYSMGGLYVDYDQMTNIKGLFAAGECDFSQHGGNRLGANSL
                     LSAIYGGTVAGPNAIDYISNIDRSYTDMDESIFEKRKAEEQERFDKLLAMRGTENAYK
                     LHRELGEIMTANVTVVRENEKLLETDKKIVELMKRYEDIDMEDTQTWSNQAVFFTRQL
                     WNMLVLARVITIGAYNRNESRGAHYKPEFPERNDEEWLKTTMASFQGAFEKPQFTYDD
                     VDVSLIPPRKRDYTSKSKGGKK"
     gene            complement(5151..5765)
                     /locus_tag="P6L01_001088"
     CDS             complement(5151..5765)
                     /locus_tag="P6L01_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HDP3030169.1"
                     /translation="MAQSKNEFYLRRIHSLLGIIPIGAFLVVHLLVNHQATQGAEAFN
                     KASNFMESLPFLIIVEFLFIYIPLLYHGLFGIHIAFTAKENVGHYSIFRNWMFFFQRV
                     SGILTFIFIGIHLWQTRLQKAFYGKEVNYDLMHETLQHPGWAIFYIICIIAVVFHFAN
                     GLWSFLVTWGGLQSPKSQRVFTWVSLIVFLVISYIGVTAIIAFM"
     gene            complement(6089..7870)
                     /gene="uvrC"
                     /locus_tag="P6L01_001089"
     CDS             complement(6089..7870)
                     /gene="uvrC"
                     /locus_tag="P6L01_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDP3030170.1"
                     /translation="MEDYKQRIKNKLNVVPMEPGCYLMKDRNDQVIYVGKAKKLRNRL
                     RSYFTGAHDAKTTRLVGEIRRFEFIVTSSETESLLLELNLIKQYQPRYNILLKDDKSY
                     PFIKITKEKYPRLLVTRTVKQGTGKYFGPYPNAYSAQETKKLLDRIYPYRKCDKMPDK
                     LCLYYHIGQCLGPCVYDVDLSKYAQMTKEITDFLNGEDKTILKSLEERMLTASESLDF
                     ERAKEYRDLIQHIQNLTNKQKIMSSDKTIRDVFGYCVDKGWMCIQVFFIRQGNMIKRD
                     TTMIPLQQTEEEEFYTFIGQFYSLNQHILPKEVHVPRNLDKEMIQSVVDTKIVQPARG
                     PKKDMVDLAAHNAKVSLNNKFELISRDESRTIKAIEELGTQMGIQTPIRIEAFDNSNI
                     QGVDPVSAMVTFVDGKPDKKNYRKYKIKTVKGPDDYKSMREVVRRRYSRVLNEGLPLP
                     DLIIVDGGKGHMNGVIDVLQNELGLDIPVAGLQKNDKHQTSELLYGASAEIVPLKKNS
                     QAFYLLHRIQDEVHRFAITFHRQTRQKTGLKSILDDIDGIGNKRKTLLLRSFGSIKKM
                     KEATLEDFKNIGIPENVAKNLHEQLHK"
     gene            8036..8131
                     /locus_tag="P6L01_001090"
     CDS             8036..8131
                     /locus_tag="P6L01_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030171.1"
                     /translation="MLDYHLTFIDLLIPPFSSRKQYDDLSFNFLY"
     gene            complement(8194..8508)
                     /gene="trxA"
                     /locus_tag="P6L01_001091"
     CDS             complement(8194..8508)
                     /gene="trxA"
                     /locus_tag="P6L01_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDP3030172.1"
                     /translation="MAIVKVTDADFDSKVESGVQLVDFWATWCGPCKMIAPVLEELAA
                     DYEGKADILKLDVDENPSTAAKYEVMSIPTLIVFKDGQPVDKVVGFQPKENLAEVLDK
                     HL"
     gene            complement(8681..11029)
                     /locus_tag="P6L01_001092"
     CDS             complement(8681..11029)
                     /locus_tag="P6L01_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDP3030173.1"
                     /translation="MRQKTLDVLEFEKIKSLVANETISDLGLEKVNQMMPATNFETVV
                     FQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADIGGVLNVSELNLIKRLIQVQNQF
                     KTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFHQINETCDTYDLYDNASYELQGIRS
                     KISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQS
                     ASGQTLYIEPSSVVEMNNQISRLRHDEAIEKERILTQLTGYVAADKDALLVAEQVMGQ
                     LDFLIAKARYSRSIKGTKPIFKEERTVYLPKAYHPLLNRETVVANTIEFMEDIETVII
                     TGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSVFKNVYCDIGDEQSIEQSLST
                     FSSHMTNIVEILKHADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTH
                     YPELKAYSYNREGVMNASVEFDVDTLSPTYKLLMGVPGRSNAFDISKKLGLSLNIINK
                     AKTMIGTDEKEINEMIESLERNYKCVETQRLELDRLVKEAEQVHDDLSKQYQQFQNYE
                     KSLIEEAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEA
                     KSIKQNVQKQKYDKIVAGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLE
                     KKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIELDQYLDQAVLSNYEQVYIIH
                     GKGTGALQKGVQQHLKKHKSVSDFRGGMPSEGGFGVTVATLK"
     gene            complement(11039..12751)
                     /gene="polX"
                     /locus_tag="P6L01_001093"
     CDS             complement(11039..12751)
                     /gene="polX"
                     /locus_tag="P6L01_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HDP3030174.1"
                     /translation="MTKKDVIKLLEQIATYMELKGENTFKISAYRKAAQSLELDERPL
                     DEISDVTELKGIGKGVAEVINDYRETGESQYLQQLQEEVPEGLIPLLKIQGLGSKKIA
                     KLYKELNIVDKASLQVACENGKVSELSGFAKKTEQNILEAVKQLGAKKDRYPIDQMRR
                     LNQEIIDYIDTLNYIDQYSSAGSFRRFKEMSKDLDFIISTDNPKAVQQQLLNIPNKVK
                     EVAVGNTKVSLELAYDDETIGVDFRLIEPRAFYHTLQHFTGSKEHNIRIRQLAKARDE
                     KVSEYGIEQADGTLIQYDSEAKIYEHFNVNFIPPAMREDGSEFDKDLSNIITLDDING
                     DIHMHTTYSDGAFSIRDMVEANIAKGYKFMVITDHSQSLRVANGLQVERLLRQNEEIK
                     ALDKEYSEIDIYSGTEMDILPDGSLDYDDEILAQLDYVIGAIHQSFNQSEEQIMERLA
                     NACRNPYVRHIAHPTGRIIGRRDGYKPNIEQLMALAEETNTVLEINANPHRLDLNAEI
                     VRKYPNVKLTINTDAHHTNHLDFMNYGVATAQKGFVTKDRVINALSREAFKDFIENNI
                     KLKK"
     gene            complement(12824..13345)
                     /locus_tag="P6L01_001094"
     CDS             complement(12824..13345)
                     /locus_tag="P6L01_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDP3030175.1"
                     /translation="MVIDFIIIIFFVYFVIVGFRRGFWLSMIHLSATIVSLWIASQFY
                     KSIVERLIVFIPYPKTTAFNTTFAFHFNHLQNRFEAIVAFLMITLFCKFILYLIIVTF
                     DKIIAYQNIHIFSRAMGMIVGVFMTIIVLHFTLYLLALYPNEALQHQLKISIVSHSLI
                     FHIPYLSAFTINL"
     gene            complement(13346..13612)
                     /gene="zapA"
                     /locus_tag="P6L01_001095"
     CDS             complement(13346..13612)
                     /gene="zapA"
                     /locus_tag="P6L01_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDP3030176.1"
                     /translation="MAQFKNKVNVSINDQLFTIVGEDNPEHIRYVAHLVDDKIKELGY
                     KAAGLDTSRKAILTAVNIMHEKVLLEEENRRLKQQIHKLQQREQ"
     gene            13982..>14738
                     /locus_tag="P6L01_001096"
     CDS             13982..>14738
                     /locus_tag="P6L01_001096"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDP3030177.1"
                     /translation="MANIVFKLSDKDITTLMSRITFDTENLPQGMKARAKYQNTTVNI
                     YQSGKVMFQGNHAEAVSKELLPQHSQLNTNKTKKKNMANSSLEQTLMYDQFNCIGSDE
                     AGSGDYFGPLTVCAAFVTKEHVPILKTLGVDDSKKLTDTKIVELAEQLVAFIPHSLLT
                     LHNDKYNIQQAKGWTQVKMKAVLHNEAIKNVLEKIDSSQLDYIVIDQFAKREVYSHYA
                     LSDIPLPKKTKFETKGESKSLAIAVASIISRYAF"
BASE COUNT         4555 a         2796 c         2050 g         5337 t
ORIGIN      
        1 tgtgtcacta catttctgaa tttcaagaat tttgctactc catattttaa ccatcaaatt
       61 attgatataa tttatgactt tagaaatcct tttaaatatg tatacgattc atgattttaa
      121 ctaaaatttc aaatttcctg acttcttgga atcttaattt tatacttttt aataatacac
      181 ttttaaccaa ataatcaaca tgattcaacc atatagggtt atcaacaatc acattctcca
      241 aaattacaat tcttttcata acataaatgt gttattcatt ccaattatct tataaaattg
      301 tcagaaatac ttttaaacaa acattcatga cacgacctcc tagttaaatc agtctataaa
      361 ctaaaaaaag ccatagctac ttctatttaa aaagtagtat gactgattgt ttcgtgatgt
      421 cgatttttga aattgatggt gccatgtaaa gtttgatcat caataataac ttcagcatat
      481 gtttcttcca ttgaacttct agattgagat atacttccag gattaataac atgaacacca
      541 ttaatatact catattttgc cacatgtgta tgtccataaa atgcaaacaa acaacctaat
      601 tcaagtcctt tttcagctaa taaatctctt gttcgattga cttgatataa atgtccatga
      661 gtataaaatg ctttcacgtc attatatttt gcgaccgctt cattttcaaa ttctgggtaa
      721 aaatcacaat tgccttttac tctattaaat aagctaagtt ccgtatcatc atacgcgaat
      781 tctgaatctc ctaaatgtaa atatacatct gcatctgggt gcatttcata aatttgatat
      841 aaaacgcctg attcagtatg gttatcactc acaataatcc atttagacat ttttttcacc
      901 ttcaagaaaa gcttgaagta aattaatcgc atttcgtcta tgactaattt gccctttttg
      961 ttcttttgaa agttgagcca tggttttatc taatttcggt acataaaata tcggatcata
     1021 tccgaaacca ttttcgccat attttccatc tgcaatttca cctgaaacag tacctttaaa
     1081 tacttttgtt tccatatcag ggccactcat acttatgaca caaacaaatt gcgcacgacg
     1141 atcagttgta ttaccaagct tatttaataa tttttcaata tttgcttcat cgcttttatt
     1201 ttcaccagca taacgtgcag agtatatacc tggctcacca tttaatgcaa aaacttctag
     1261 tccactgtca tcagctatga cagttttatt caatgctttt gcagcagctt ctgattttaa
     1321 tatagcattt tcttcaaatg ttgatcctgt ttcttccaca tcaaaatctg gtattagttc
     1381 tgaaatacct attacgtggt aatctggaaa tattacttta aagtcattta ttttcccttg
     1441 attattcgat gcaataacaa tctctttcat taaaaatcct cctagtcatt cactgatata
     1501 cgttccacat tgacagataa atttaaccac tctttgataa tgttagtaat gtgagtagga
     1561 tcacctgttg caaaaaatcg atgatctgga tgttcagtat aacttgcatg ttcattacta
     1621 aatgttagca atgcactaac ttcacgagcc gtttctaatc cagacgaaat cactgtcttt
     1681 ttaccaccaa aataatcata gataggttta tagagcaatg gatagtgggt acatcctaaa
     1741 atgacagtat cagactcact attacgccaa cgtttcagtg tttgatgaat gacaatgctt
     1801 gtaattgttg gatcactata tctcatttgt tctacaagtg gcacaaaacc tggacaggca
     1861 acgccatgta cctctacatg tggattgata cgtttaatat gcgtacgata tgcttcagat
     1921 ttaattgtgc cttcagttcc tagtactaat acattttgat ttctagtagt cattattgct
     1981 gttctagcac ctggttcaat tacaccaatc actgggattg ataaggtctt ttgtaaatat
     2041 tctaaagcta cagcagttgc tgtattacaa gcaatcacga gcatttttat atcaaattcc
     2101 attaatttac gagcgatttc aactgtatat tgttttactt gttctcctgg tcttggccca
     2161 tatggacatc gtccaatatc acctaagtaa taaatcgtct catttggcaa ctgacgcata
     2221 atttctttag ctactgtcaa acctccgaca ccagagtcta ttacacctat tggtttattc
     2281 atattcggtc atccttactt ttaatttaat ttttagttta tcataactaa gcattggatt
     2341 ttagtattat gcactgtgtt taccattttt gtcattataa tatttatttt aaatcagccc
     2401 actatcatat tgtcatgtaa tctgcttatt aaaaaaatcc cttccaagtt attgtgtatc
     2461 tccattcaat ttaattttga aaggaacata acattttaac tcaaaaggga ttaattttta
     2521 ttctacttca tggtctgaac caaagaatga tttaaacatg tggaatgttg tttctctgtt
     2581 cattgctgca atggatgttg ttaatggaat acctttaggg caagcattaa cacagttttg
     2641 tgaattacca cactgctgta agccaccagt acccattaat gcatttaaac gttcatcttt
     2701 agtcatagat cctgttgggt gcaaattaaa caaacgaact tgcgagattg cttgtgcacc
     2761 aacgaattta ttattttcag taacattagg acaaacctct aaacatacac cacatgtcat
     2821 acatttagat aattcataag ctgtttgacg ttttttctct ggcatacgtg gtcccggacc
     2881 taaatcatac gttccatcaa ttgggatcca tgctttcata cgttttaagt tatcgaacat
     2941 tctagaacga tcaacttgta agtcacggat aactgggaaa gtattcattg gctctaaacg
     3001 aataggttgt tctaattgat caacaatcgc agaacaagat tgtcttgcac gaccattgat
     3061 aaccatagaa catgctccac atacttcttc taagcagttc atatcccaga caacaggtgt
     3121 tgttttttca cctttaatat taactgggtt acgtctaatt tccattaaac aagcaatgac
     3181 gtttaaattt tcacgatatg gaatttcaaa tgtttcttca taaggcttag aatcacttgt
     3241 atcttgtcgt ttaataatta attttactgt tttttgtttc ggtttagatt gtgtttcatg
     3301 ttgtggagtg tttttcactg attgttcagt cattattttt tacccccttt agacttactt
     3361 gtgtaatcac gtttacgagg tggtattaaa ctcacatcga cgtcatcata agtaaactgt
     3421 ggtttttcaa atgcgccttg gaatgaggcc attgtcgttt ttaaccactc ttcatcatta
     3481 cgctctggga attctggttt ataatgggca ccgcgtgatt cgttacggtt atatgcacca
     3541 atcgtaataa cacgtgcaag tactaacatg ttccatagtt gacgggtaaa gaataccgct
     3601 tggttactcc aagtttgagt atcttccata tcaatatctt cataacgttt catcaattca
     3661 acaatctttt tatctgtttc taacagtttt tcattttcac gaacaacagt tacatttgct
     3721 gtcataattt caccaagttc acggtgtaat ttataggcat tttctgtacc gcgcatagct
     3781 aataatttat caaaacgttc ttgctcttca gctttacgtt tttcaaaaat actttcgtcc
     3841 atatcagtat atgatcgatc aatatttgaa atataatcaa tcgcgtttgg acctgctact
     3901 gtaccaccat aaatcgctga taacaatgaa ttggcaccta agcggttacc accatgttga
     3961 gagaagtcac attctccagc tgcaaataac cctttaatat ttgtcatttg atcataatct
     4021 acatatagac cacccattga atagtgaaca gctgggaaaa tcttcattgg tactttgcgt
     4081 gggtcatcac cagtgaattt ttcataaatt tcaatgatac cacctagttt tacatctaac
     4141 tcatgtggat ctttatgtga caaatcaaga tataccatgt tttcgccatt tatacctaat
     4201 ttttggttaa tacatacatc gaaaatttca cgcgttgcga tatcacgagg tactaagtta
     4261 ccataatcag gatatttctc ttctaagaag taccaaggct taccatcttt atatgtccaa
     4321 attcgtccac cttcaccacg tgctgattca ctcattagtc gcagtttatc atcaccaggg
     4381 attgcagtag gatgaatttg aatgaactca ccattagcat aaatagcgcc ttgttggtaa
     4441 acaatggaag ccgctgatcc tgtattaatc attgagtttg ttgttttacc gaaaataata
     4501 ccagggccac ccgttgccat aataactgca tctgaaccaa atgtttcaat ctcagcagtt
     4561 gtcatatttt gtgcaacgat acctcttgca ctatcatcgt cacctttaac tatgccaaga
     4621 aattcccatc cttcatactt cgtaactaat ccatctactt catatgcacg aacttgttca
     4681 tccaatgcat ataataattg ttgtccagtt gttgcccctg catatgctgt tctgtggtgt
     4741 aatgtaccac cgaaacgtct aaaatctaat agaccttcat ttgttctatt gaacattaca
     4801 cccatacggt ctaataaatg aataatttta ggtgctgcct ctgtcatcgc tttaacaggt
     4861 ggttggtttg caaggaagtc gccaccatac actgtatcat caaagtgaat ccaaggagaa
     4921 tcgccttccc ctttagtatt gaccgcacca ttaatgccac cttgggcaca aacagagtgc
     4981 gaacgcttta ctggtacaac tgagaacaaa tctacatgtg cacctttttc tgccgcttta
     5041 attgttgaca ttaagcccgc taggccacct ccgacaacaa taagatgttt ctctgccata
     5101 aaaatttcac tcccctaaat tttcaatcta tatttgttaa atgcgatgta ttacataaag
     5161 gcaataattg cagtaacacc aatatacgaa ataactaaaa atacgattaa tgaaacccat
     5221 gtaaatactc gttgtgattt tggagattga agtccacccc aagtaactaa gaatgaccat
     5281 aagccatttg caaagtggaa cacaacagca ataatacaaa taatataaaa tattgcccat
     5341 ccaggatgtt gcaatgtttc gtgcattaaa tcgtaattca cttctttgcc gtaaaatgct
     5401 ttttgtaaac gtgtttgcca taaatggata ccaataaaga taaatgttaa gataccactc
     5461 actctttgga agaagaacat ccagtttcta aaaatcgagt aatgtccaac attttctttt
     5521 gctgtaaatg caatgtgtat accaaacaaa ccgtgatata acaacggaat gtatataaat
     5581 aaaaattcta caataattag aaatggtaat gattccataa agttagatgc cttattaaac
     5641 gcttcagcac cttgtgttgc ttggtgattc actaataaat gaacgaccaa aaatgcacct
     5701 attgggataa tacctaataa cgagtgaata cgtcttagat aaaattcatt ttttgattga
     5761 gccaaaagga gtcccccctg tgaacgaata tttaatttat tgagctattt atattaaacg
     5821 tacgcttaac cccctaaagt gatatcgttt ctagcgttgt cattatcatt aagcgaaaca
     5881 ttttaaagac aaatacacac tgtacgatca ccaaactgca tgtcgaacaa tgtaacattt
     5941 ggattcgata tttaaaattg cttgtgatga taaactttct catttagaaa acgcttccac
     6001 gtacattcaa aaaaataact ttgttaacca tattgtaaca ttatttcata tattttgggg
     6061 catgagaatg attctcacgc ccagtaattt atttatgcaa ttgttcatgt aggttctttg
     6121 cgacgttttc aggaatacct atatttttaa aatcttcaag tgtagcttcc ttcattttct
     6181 tgattgaacc gaatgaacgc aataataatg ttttacgttt gttaccgata ccatctatat
     6241 catcaagtat tgatttcaag cctgtctttt gacgtgtttg tctatgaaat gtgattgcga
     6301 atctgtgaac ctcatcttgg atacgatgca acaaataaaa tgcctggcta tttttcttca
     6361 gtggtacaat ttctgcacta gcgccatata ataattcaga tgtttggtgt ttatcatttt
     6421 tctgcaaacc tgcaacaggg atatcaagac ctaattcgtt ttgtagcaca tcaataaccc
     6481 cgttcatatg tcctttacca ccatcgacta ttattaaatc aggtaatggt aatccttcgt
     6541 ttaaaacgcg agaatatcgt cgtcttacta cttctctcat tgatttgtaa tcatctggac
     6601 ctttaaccgt tttgatttta tactttctat aatttttctt atctggttta ccgtcgacaa
     6661 atgtaaccat tgctgacact ggatccacac cttgaatatt agaattatcg aatgcttcaa
     6721 ttctaattgg tgtttgaatt cccatttgtg ttccaagttc ttcaatagct ttaatcgttc
     6781 ttgactcatc acgtgatatt aattcaaatt tattatttaa ggatacttta gcgttatgtg
     6841 cagctaggtc aaccatatct tttttgggac ctcgcgcggg ttgaacgatt ttagtgtcca
     6901 caacagattg aatcatttct ttatccaaat tacgtggtac atgaacttcc ttaggtaaaa
     6961 tatgttggtt taagctataa aattgtccaa taaatgtata aaattcttct tcttctgttt
     7021 gctgtaatgg aatcatcgtt gtatctcgct ttatcatatt accttgtcgt ataaagaaaa
     7081 cttggataca catccatcct ttatcaacac aataaccaaa gacatcacga atcgttttat
     7141 ctgatgacat aattttttgt ttgtttgtca gattttgaat atgttgaatt aaatctctat
     7201 attctttagc ccgttcaaaa tcaagtgatt cacttgcagt taacattcgc tcttctaaac
     7261 tttttaaaat tgttttgtct tccccattca gaaaatcagt aatttccttc gtcatttgtg
     7321 cgtatttact caaatcaacg tcatatacac atggtcctaa acattgtcca atatggtaat
     7381 aaagacataa tttatctggc atcttatcac atttgcgata tggatatatt ctgtctaata
     7441 actttttagt ttcttgagca gaatatgcat tcggatacgg tccgaaatat ttgccagtac
     7501 cttgttttac agttctcgtc actagtagtc taggatattt ctccttcgta attttaataa
     7561 atggataact tttatcatcc tttaataata tattatatct tggttgatat tgttttatca
     7621 gattcaattc cagtaaaagt gactctgttt cacttgacgt gacaataaac tcaaaacgac
     7681 gtatttcacc aaccagtctc gttgttttag catcatgagc acccgtaaaa tatgatctca
     7741 atcgatttct tagcttttta gctttgccaa catatatcac ttgatcatta cgatctttca
     7801 ttaaatagca gcctggttcc ataggtacga catttaattt atttttaatt cgttgcttat
     7861 agtcttccaa aaatatcccc ccttctaatt ttaaaatgaa tgatgctaca aaagtgcttc
     7921 aaatttatca ccactaattc tagctcataa tcaacatctt cgagtatgac ttttcatgca
     7981 catcatgtaa tattgaacat ggttttatca aaccaactgc tacaaatcta tcagcatgct
     8041 agattatcat ttaacgttca tagatttatt aatcccacca ttctcaagtc gtaaacaata
     8101 tgatgactta tcattcaact ttttatatta aaaacacaaa caaaaactgg agcaattcat
     8161 taaagaaatg ccccagtcgt cattggttat aacttataaa tgtttatcta aaacttcagc
     8221 taagttttct tttggttgga aaccaacaac tttatcaact ggttgaccgt ctttaaagac
     8281 gattaatgtt ggaatactca tcacttcata tttagctgca gttgatggat tttcatcaac
     8341 atctaatttt aaaatgtcag ctttaccttc atagtcagct gctaattctt ctaataccgg
     8401 agcgatcatt ttacatggac cacaccatgt tgcccaaaaa tctactagtt gtacaccaga
     8461 ttctactttt gaatcaaaat ctgcatctgt tacttttacg attgccataa atgccaatcc
     8521 tccttaatta ttattatgat gttcaaatta tatcagataa catgcttcat gtatttctat
     8581 ttgctcaatt tttatgctta tttcaaataa catgtttgaa atgtattctt gttgctttac
     8641 attattttaa ctgcagctat ttaatttatc aaattataat ttattttagt gttgcaacgg
     8701 taacgccaaa tccaccttcg cttggcatac cacctctaaa gtcactaaca cttttatgct
     8761 tcttcaaatg ttgttgtaca cctttttgaa gtgcacctgt acctttacca tgaatgatat
     8821 aaacttgttc gtaattactt aaaacggctt gatctaaata ttgatctagt tcaattaaag
     8881 catcctcata acgatagcct cgtaagtcaa gttcagtttt aattgtttga cgattttgac
     8941 gtgtaaccat tttcgttggc ttaacttttt ctttttgttt tttctctaaa tcttcaatag
     9001 gtaacttcat tttaataatt cccatttgaa caattgcttc ttcatcattg acaatttcta
     9061 aaacttcacc cttttgaccg taagataata cttttacttc atcaccagca acaattttat
     9121 cgtatttttg cttttgtaca ttttgcttta tagatttcgc ttcataatga tcatctaatc
     9181 gtttcttctt atcaatcaat tcatgttctt taacatctgc acctttttgt tcacgcaatt
     9241 gtcttaagtc tttaataata tcgtcagctt cttttgttgc agctttaatc ttctgatttg
     9301 ctttttcttt cgcttcctct attagagact tttcataatt ttggaattgt tgatactgct
     9361 tagataaatc atcatgcact tgctccgctt cttttacaag acggtccagt tctaacctct
     9421 gtgtctctac acatttataa ttacgctcta atgattcaat catttcattt atttcttttt
     9481 catcagtacc aatcatcgtc ttagccttat taataatatt caaactaaga cctaactttt
     9541 tagaaatgtc aaaagcattt gaacgacccg gcacacccat taataactta tacgttggac
     9601 tcaaagtatc tacatcaaat tctacactcg cattcataac gccttctcga ttataactat
     9661 atgctttaag ttcaggatag tgcgtcgttg ccattactag agaaccaatt tttctaacat
     9721 gatctaaaat gctcattgct aatgcagcac cttcacttgg atctgtacct gcacctaatt
     9781 catcaaataa aactaaacta tgtttgtctg catgctttaa aatttcaact atattcgtca
     9841 tatgagatga aaaagttgat aatgattgtt ctattgattg ttcatctccg atatcgcaat
     9901 atacattttt aaatacactt aactgactac catcaagtgt gggaatcaac aatcctgatt
     9961 gagccataac aataattaaa cctaatgttt ttaatgttac agttttacca cctgtattcg
    10021 gtcctgtaat aattaccgtt tcaatatctt ccataaattc gatggtatta gctacaacag
    10081 tctcacgatt taataatgga tggtatgctt taggtaaata tacagtacgt tcctctttaa
    10141 atatcggctt tgttccttta atacttctac tatatctcgc ttttgcgatt aaaaaatcta
    10201 actgccccat gacttgttct gccacaagta gtgcatcttt gtccgcagcc acataaccag
    10261 ttagttgcgt taaaatgcgt tctttttcaa ttgcttcgtc atgacgtaat cgactaattt
    10321 gattattcat ttcaacaact gatgatggct caatatacaa tgtttgtcct gaagcagatt
    10381 gatcatgtac aatcccatta aaatcttgtc gatattcagc tttgacaggt ataacgtttc
    10441 tttcattcct aactgttaca atagcatctg ataatttttt ctgatttgct tgacttttaa
    10501 caatacggtc caaattttgt ctaatacgtt gattcgtgct agaaatttta cttctaatcc
    10561 cttgcaattc ataactcgca ttatcatata aatcatacgt atcgcatgtt tcatttattt
    10621 gatgaaaaag atcagttaac acaggtaatt gattcatctt gtcatctaat attgggtatt
    10681 taacaccttc atcttcttca accaattgat tataaaatgt cttaaattga ttttgtactt
    10741 gaattaatct ttttatcaag ttaagctctg atacatttaa aacgccgcca atatcagcgc
    10801 gatgaatgaa tgctgatact ttagataagc cactcaagct tggtaaacga tgcttattat
    10861 agatttgagc aatctcatcc gtttcttcca tttgaaaaac aaccgtttca aaattagtag
    10921 ctggcatcat ttgattgacc ttttccaagc ctaagtcact aatagtttca ttggcaacga
    10981 gtgattttat tttttcaaat tctaagacgt ctaatgtttt ttgtctcata aaatccctct
    11041 atttcttaag ttttatatta ttttcaataa agtctttaaa agcttcacgc gataatgcgt
    11101 taatcactct atcttttgtt acaaatcctt tttgcgcagt tgctacgcca taattcataa
    11161 aatctaaatg atttgtatga tgcgcatcag tgttaatagt taatttcaca tttggatatt
    11221 tacgaacgat ttcagcgttc agatccagtc gatgtggatt ggcattaatt tctaatactg
    11281 tattcgtttc ttcagctaat gccattaatt gttcaatatt cggtttataa ccatctcttc
    11341 tacctataat acgccctgtt ggatgcgcta tatgtcgcac gtatggattg cgacatgcat
    11401 tagctaatcg ttccataatt tgttcttctg attggttaaa gctttgatga atagctccaa
    11461 ttacataatc aagttgtgct aaaatttcat catcataatc cagcgagcca tcaggtaata
    11521 tatccatttc tgtacctgaa taaatatcaa tttcactata ttctttatct aaagccttaa
    11581 tttcttcgtt ttgtcttaaa agtctttcca cttgtaagcc attagcaaca cgtaaacttt
    11641 gtgaatgatc agtaattacc atgaatttat aaccttttgc gatatttgct tctaccatgt
    11701 ctcgaataga aaacgcacca tcactatacg ttgtatgcat atgaatatca ccattaatat
    11761 catctaatgt aatgatatta cttagatctt tatcaaattc gctaccatct tctcgcatag
    11821 caggtggtat aaaattcaca ttaaaatgtt catatatctt ggcttcacta tcatattgaa
    11881 ttaatgtacc atcagcttgt tcaattccat attcacttac tttttcatca cgtgctttag
    11941 caagttgtcg aattcttata ttatgttctt ttgacccagt aaaatgttgc aatgtatgat
    12001 aaaaagccct tggttcaatt aatcgaaaat cgacaccaat cgtttcatca tcatacgcta
    12061 attctaatga aacttttgtg ttccccactg caacttcttt tactttattg ggaatattta
    12121 ataattgctg ctgcactgct tttgggttat cggtacttat tatgaaatct aaatctttgc
    12181 tcatttcttt aaaacgacgg aagcttcctg cagatgaata ttgatcgata taatttaatg
    12241 tatctatata atcaatgatt tcttgattaa gtcttctcat ttgatcaatt ggatatctat
    12301 ctttcttagc accaagttgt ttcacagctt ctaatatgtt ttgttccgtt ttcttagcaa
    12361 atccgcttaa ttcactaact tttccatttt cacaagcaac ttgaagtgac gctttatcaa
    12421 caatattcaa ctctttatat agcttagcaa ttttcttgct tccaagtcct tgaattttca
    12481 aaagtggaat aagaccttcc ggaacttctt cctgtaattg ctgtaaatac tgagattcac
    12541 cggtctcacg gtaatcattg attacttctg caacaccttt accaatgcct tttaactccg
    12601 ttacatcaga tatttcatct aatggtcgtt catctaattc aagactttga gctgcttttc
    12661 gatacgctga tattttaaaa gtattttccc cttttaattc catataagta gcaatttgtt
    12721 ctaatagttt gataacatct ttttttgtca taataacact ccataaaaag aagaccagga
    12781 cgtatcatta atatatacct ttgtcctgac ctcttatgat aatttataaa ttaatggtga
    12841 aagccgataa atatgggatg tgaaaaatca atgaatgact cacaatagat attttaagct
    12901 gatgttgtaa tgcttcgtta ggatataatg ccaatagata taacgtaaag tgtaagacaa
    12961 ttatcgtcat aaacacacca actatcattc ccattgcacg actgaaaata tgaatgtttt
    13021 gatacgctat tattttatca aaagttacga taattagata taaaatgaac ttacaaaaca
    13081 atgtaatcat taaaaaagct acaatcgctt caaatcgatt ttgtagatga ttaaaatgaa
    13141 acgcaaaagt tgtattaaat gctgttgttt taggatatgg aataaataca attaatcttt
    13201 ctacaataga tttgtaaaat tgactggcaa tccacaatga tacaatcgtt gcactcaaat
    13261 gtatcataga taaccaaaaa cctcgtctga atccaacgat gacaaaatac acaaagaaaa
    13321 tgattatgat aaaatcaatg accatttatt gctcacgctg ctgcaatttg tgaatttgtt
    13381 gtttcaaacg tcgattttct tcttctagta gtactttttc atgcataata ttcacagcag
    13441 ttagtattgc ttttcttgaa gtatctaaac ctgctgcttt ataccctaat tcttttattt
    13501 tatcatcaac taaatgtgct acatatcgta tgtgctctgg gttatcttcc ccaacaattg
    13561 taaaaagctg atcattaatt gatacattta ccttgttttt aaactgtgcc atttataatt
    13621 tctcctgatc ctttttttaa aatctaaatt cacgttataa aatatgactg gatagtttgt
    13681 ctgaatttga tactaatatt gttatattgc aattatgata aaacaacaac acaatctcta
    13741 tagatgactt aatgttcttt ttataatgaa ataatgtaaa gaattttcta ttcaatactt
    13801 tatcatgttt aaattgtgtc actataacat tttcataaac attatacatg accactatgt
    13861 attttgtaag tatccgcaat taattcttta caacatacat aaatgtttct gacgttatta
    13921 tcatttatga tatgattatt tttgctaatg ataatgaaat tatatgaaag gatttacaca
    13981 aatggcgaat atcgttttta aattgtcgga taaagacata acgacattaa tgtcacgcat
    14041 tacttttgat actgagaatt tacctcaagg tatgaaagca cgtgcaaagt atcaaaatac
    14101 aactgtaaat atttaccaat caggtaaagt catgtttcaa ggtaatcatg cagaagctgt
    14161 gtctaaagaa cttctaccgc agcattctca attgaatact aataaaacta aaaagaaaaa
    14221 tatggctaat tcttctcttg aacaaacttt aatgtatgat caatttaact gtattggtag
    14281 tgatgaggct ggaagtggcg attactttgg tcctttgact gtgtgtgctg cttttgttac
    14341 caaagagcat gttcctattt taaaaacttt gggtgtagat gattcaaaaa aattaaccga
    14401 taccaaaatt gtagaacttg ctgagcaatt ggttgctttc atcccacatt cactattaac
    14461 actacacaat gataaatata atattcagca agcaaaaggc tggactcaag taaaaatgaa
    14521 agctgttctt cataatgaag ccatcaaaaa tgtacttgaa aaaattgatt cgtctcaatt
    14581 agactatatc gtcattgatc agttcgcaaa acgcgaagtg tatagccatt acgcattatc
    14641 cgatatacca cttccaaaga aaacaaaatt tgaaacaaaa ggagaatcga agtcattagc
    14701 cattgcagtg gcaagcataa tttctagata cgcattta
//
LOCUS       DAOBBH010000048        14452 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.048, whole genome shotgun sequence.
ACCESSION   DAOBBH010000048 DAOBBH010000000
VERSION     DAOBBH010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14452
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.048"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..853)
                     /locus_tag="P6L01_001097"
     CDS             complement(<1..853)
                     /locus_tag="P6L01_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDP3030178.1"
                     /translation="MDVIKQIQQAIVYIEDRLLEPFNLQELSDYVGLSPYHLDQSFKM
                     IVGLSPEAYARARKMTLAANDVINGATRLVDIAKKYHYANSNDFANDFSDFHGVSPIQ
                     ASTKKDELQIQERLYIKLSTTERAPYPYRLEETDDISLVGYARFIDTKYLSHPFNVPD
                     FLEDLLIDGKIKELRRYNDVSPFELFVISCPLENGLEIFVGVPSERYPAHLESRFLPG
                     KHCAKFNLQGEIDYATNEAWYYIESSLQLTLPYERNDLYVEVYPLDISFNDPFTKIQL
                     WIPVKQSP"
     gene            complement(1083..2567)
                     /locus_tag="P6L01_001098"
     CDS             complement(1083..2567)
                     /locus_tag="P6L01_001098"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDP3030179.1"
                     /translation="MSTKNKHIPCLITIFGATGDLSHRKLFPSIFHLYQQDNLDEHIA
                     IIGIGRRDITNDDFRNQVKSSIQKHVKDTNKIDAFMEHVFYHRHDVSNEESYQELLDF
                     SNELDSQFELKGNRLFYLAMAPQFFGVISDYLKSSGLTDTKGFKRLVIEKPFGSDLKS
                     AEALNNQIRKSFKEEEIYRIDHYLGKDMVQNIEVLRFANAMFEPLWNNKYISNIQVTS
                     SEILGVEDRGGYYESSGALKDMVQNHMLQMVALLAMEAPISLNSEDIRAEKVKVLKSL
                     RHFQSEDVKKNFVRGQYGEGYIDGKQVKAYRDEDRVADDSNTPTFVSGKLTIDNFRWA
                     GVPFYIRTGKRMKSKTIQVVVEFKEVPMNLYYETDKLLDSNLLVINIQPNEGVSLHLN
                     AKKNTQGIETEPVQLSYSMSAQDKMNTVDAYENLLFDCLKGDATNFTHWEELKSTWKF
                     VDAIQDEWNMVDPEFPNYESGTNGPLESDLLLARDGNHWWDDIQ"
     gene            2880..3800
                     /gene="rnz"
                     /locus_tag="P6L01_001099"
     CDS             2880..3800
                     /gene="rnz"
                     /locus_tag="P6L01_001099"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDP3030180.1"
                     /translation="MEVTFFGTSAGLPTKERNTQAIALNLEPYSNSIWLFDVGEGTQH
                     QILHHAIKLGKVTHIFITHMHGDHIFGLPGLLSSRSFQGGEQKPLTLVGPKGIKAYVE
                     MSMNLSESHLNYPITYIEIDDHLTYHHDGFTVEAHLLNHGIPSYGYRVMAPETTGTIN
                     VEALKNIGLEPGPKYQEVKSHDTFEHNGQVYQSKDFRGESKQGPVVAIFGDTKPCSNE
                     RVISRDADVMVHEATYIDGEKHLANNYHHSHIEDVFALIKEANVKRTLITHLSNRYNT
                     EDINEIYQILIQNEDTPNFNFVKDFDSFKV"
     gene            complement(3903..4718)
                     /gene="proC"
                     /locus_tag="P6L01_001100"
     CDS             complement(3903..4718)
                     /gene="proC"
                     /locus_tag="P6L01_001100"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDP3030181.1"
                     /translation="MKLVFYGAGNMAQAIFTGIINSSNLDANDIYLTNKSNEQALKAF
                     AEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPID
                     YIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVS
                     EDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSD
                     LSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNVEDQ"
     gene            4860..5615
                     /locus_tag="P6L01_001101"
     CDS             4860..5615
                     /locus_tag="P6L01_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDP3030182.1"
                     /translation="MKGQHFIVTGGTSGLGLSIVRKLLENKVHVTLLVRDVDKATRIF
                     EQELGKTINVIPCDLNDLKSIQALQFEDNTSFDGFIYSAGLGYFKSISDHSFSEMIET
                     YQLNLISFNVLYTVLRPYLTSNAHIVGISSQAAFSTQANAAHYGASKAGFYALMNALR
                     LESPNLHIMTVNVGPIDTPFHQKADPSMKYAKKMGEIMLDANQLAEDIIYGIKTKQLE
                     INRPKWMHHALKMYQIAPRFFERCFPKLFKNKA"
     gene            5631..5879
                     /locus_tag="P6L01_001102"
     CDS             5631..5879
                     /locus_tag="P6L01_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030183.1"
                     /translation="MNCQSESVVRLCVRYAEQLSVFEEFTVLDILGDISVDQISDGTL
                     YYTCEKFKLLVLQGNVLGVQIITNNDESTCEVKYRKMF"
     gene            complement(6095..7003)
                     /locus_tag="P6L01_001103"
     CDS             complement(6095..7003)
                     /locus_tag="P6L01_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDP3030184.1"
                     /translation="MQKNILKSGIALSELGLGCMSLGTDLKKAEQIIDCAVENGITYF
                     DTADMYDKGINESVVGKALLKYQQRDDIFIGTKVGNRLTKDGSTTWDPSKSYIKEAVK
                     GSLKRLGIDHIDLYQLHGGTIDDPLDETISAFDELKQEGIIRAYGISSIRPNVIDYYL
                     KHSQIETIMSQFNLIDNRPESLLDAIHNNDVKVLARGPVSKGLLTSNSVNVLDNKFKD
                     GIFDYSHDELGETIASIKEIESNLSALTFSYLTSHDVLGSIIVGASSVDQLKENIENY
                     HTKVGLDQIKTARARVKDLEYTNHLV"
     gene            7085..7627
                     /locus_tag="P6L01_001104"
     CDS             7085..7627
                     /locus_tag="P6L01_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDP3030185.1"
                     /translation="MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGA
                     VAVCALTPKKEVVLVKQYRKPVEKPLLEIPAGKLEDDENRVEAAKRELEEETGYIAKE
                     LTHVVDMYGSPGFCDEQLSIYFTDNLEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEI
                     EDAKTIIALQHLLLNYNHSK"
     gene            7732..8181
                     /locus_tag="P6L01_001105"
     CDS             7732..8181
                     /locus_tag="P6L01_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDP3030186.1"
                     /translation="MEERLNRVKQQLQQSSYKLTPQREATVRVLIENEKDHLSAEDVY
                     LKVKDKAPEIGLATVYRTLELLAELKVVDKINFGDGVARFDLRKEGAKHFHHHLVCME
                     CGRVDEIDEDLLPEVENRVENEFNFKILDHRLTFHGVCETCQAKGKG"
     gene            8230..9117
                     /gene="xerD"
                     /locus_tag="P6L01_001106"
     CDS             8230..9117
                     /gene="xerD"
                     /locus_tag="P6L01_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDP3030187.1"
                     /translation="METIIEEYLRFIQIEKGLSSNTIGAYRRDLKKYQDYMTEHHISH
                     IDFIDRQLIQECLGHLIDQGQSAKSIARFISTIRSFHQFAIREKYAAKDPTVLLDSPK
                     YDKKLPDVLNVDEVLALLETPDLNKINGYRDRTMLELLYATGMRVSELIHLELENVNL
                     IMGFVRVFGKGDKERIVPLGDAVIEYLTTYIETIRPQLLKKTVTEVLFLNMHGKPLSR
                     QAIWKMIKQNGVKANIKKTLTPHTLRHSFATHLLENGADLRAVQEMLGHSDISTTQLY
                     THVSKSQIRKMYNQFHPRA"
     gene            complement(9195..9701)
                     /locus_tag="P6L01_001107"
     CDS             complement(9195..9701)
                     /locus_tag="P6L01_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HDP3030188.1"
                     /translation="MQQALINFYYQFHTKQHYFLCHDILEDAWKAENNYSKQDAVVSL
                     ILFATACYHYRRNNLKGAYKSFNKSKEIIQNAKDRDALYLNLNDYQLLIEQQIAKLNA
                     AKPFSSVILPITPVFERIIKANYPDYDYNQETATDPFIVDHHMRRDRSEVIAAKEEAI
                     QLRKHRRN"
     gene            9793..10524
                     /locus_tag="P6L01_001108"
     CDS             9793..10524
                     /locus_tag="P6L01_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDP3030189.1"
                     /translation="MYEVKLDAFNGPLDLLLHLIQKFEIDIYDIPMQALTEQYMQYVH
                     AMKQLEINIASEYLVLASELLMIKSKMLLPQSTSDMDVDDDPREDLVGRLIEYQNYKE
                     YTAILNDMKEERDFYFTKRPTDLSHLETDESWDPNHTIDLTELIVAYQRVKNRVELNT
                     PKSVEIRKETFTIQQATEQVTSRLKDKDHFNFFSLFTFSEPIEQVVTHFLAILEMSKA
                     GIINIEQQRNFEDINIIRGVNYHFG"
     gene            10517..11059
                     /gene="scpB"
                     /locus_tag="P6L01_001109"
     CDS             10517..11059
                     /gene="scpB"
                     /locus_tag="P6L01_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDP3030190.1"
                     /translation="MDNHGILESLLFTAGDEGLDEKQLLEILDMSKDQLVELIENYSS
                     HGLMIQRFGTTYVLTTKKEAATYIEQLIEQKSQMKLSQAAMEVLSIIAYNQPLSRSDI
                     ELIRSINSDGAVKTLIAKGLVEAKVVNEQRSQQLITTDLFLNVFGISNIEDLPTTEED
                     DEEMDAFFSNLVNQKGENND"
     gene            11052..11789
                     /locus_tag="P6L01_001110"
     CDS             11052..11789
                     /locus_tag="P6L01_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDP3030191.1"
                     /translation="MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGT
                     KVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPV
                     GRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELED
                     GMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNV
                     VGLNAGEGRVLTPHEVKVMRHLAEHGK"
     gene            11922..12647
                     /gene="srrA"
                     /locus_tag="P6L01_001111"
     CDS             11922..12647
                     /gene="srrA"
                     /locus_tag="P6L01_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SrrA"
                     /protein_id="HDP3030192.1"
                     /translation="MSNEILIVDDEDRIRRLLKMYLERESFEIHEASNGQEAYELAME
                     NNYACILLDLMLPEMDGIQVATKLREHKQTPIIMLTAKGEETNRVEGFESGADDYIVK
                     PFSPREVVLRVKALLRRTQSTTVEQSEPHARDVIEFKHLEIDNDAHRVLADNQEVNLT
                     PKEYELLIYLAKTPNKVFDREQLLKEVWHYEFYGDLRTVDTHVKRLREKLNRVSSEAA
                     HMIQTVWGVGYKFEVKSNDEPAK"
     gene            12628..14379
                     /gene="srrB"
                     /locus_tag="P6L01_001112"
     CDS             12628..14379
                     /gene="srrB"
                     /locus_tag="P6L01_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SrrB"
                     /protein_id="HDP3030193.1"
                     /translation="MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETE
                     NAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQM
                     LNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIY
                     KDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDY
                     SYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESR
                     QIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNARFFVVTTSYIDK
                     IEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTE
                     PDEIKESLAVVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQ
                     QADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESED
                     ILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVK
                     SELGKGTTFIIKLPKPE"
BASE COUNT         5055 a         2143 c         2561 g         4693 t
ORIGIN      
        1 aaggactctg tttaacagga atccaaagct gaattttagt gaatgggtca ttaaatgaaa
       61 tatcgagagg gtacacttca acatataaat catttcgttc atatggcaat gttaactgca
      121 aacttgattc aatatagtac caagcttcat tagttgcata atcaatttca ccttgtaaat
      181 tgaatttcgc acaatgtttg ccaggtaaaa atcggctttc taagtgtgca ggataacgtt
      241 cacttggtac acctacaaat atttctaaac cattttcaag aggacaacta ataacaaata
      301 gttcaaatgg actaacgtca ttatatcgtc gtaactcttt aattttacca tcaatgagca
      361 agtcttctaa aaaatccgga acattaaaag gatgtgacaa atacttagtg tctataaatc
      421 gtgcatatcc aaccaatgaa atatcatctg tctcttctaa tctgtatgga tagggtgctc
      481 tctcagtagt tgataatttg atatataatc gctcttgaat ttgtaattca tcttttttag
      541 tagaagcttg aataggtgat acgccgtgaa aatcactaaa atcatttgca aaatcatttg
      601 aatttgcata gtgatatttt ttagcgatat ctacaagtcg tgtagcacca ttaatcacat
      661 catttgcagc gagtgtcatt ttacgcgcgc gtgcataagc ttctggagat aagccgacaa
      721 tcattttaaa tgattgatca agatggtatg gcgaaagacc aacgtaatca cttaattctt
      781 gcaaattgaa aggctctaat aaacgatctt caatataaac aattgcctgt tgtatttgct
      841 tgataacgtc caaaactttc actccaatta attcaatcat ttatttttat tctacattat
      901 ttctataaat tatacaccca tttgttcaat gattattaaa atagttttgg gcattgtaaa
      961 atataatttc ataatatagt ctagaaaaaa agcgaatgat agaacaattg atttacttga
     1021 ttcgtaatca atccttgtca ttcgctcatt tatttttgtt taacatgtgc gttttaattc
     1081 aattattgaa tatcgtccca ccaatggtta ccatcacgag caagtagtaa atcactttct
     1141 aatggaccat tagtacctga ttcatagtta gggaattctg gatcaaccat attccattca
     1201 tcttgaattg catcaacaaa tttccatgtt gattttaatt cttcccagtg cgtgaagtta
     1261 gtggcatcac ctttaagaca atcgaataat agattttcat atgcatctac agtattcatt
     1321 ttatcttgag cgctcattga ataagacaat tgaacaggtt cagtttcgat accttgtgta
     1381 tttttcttag catttagatg taaagataca ccttcattag gttggatatt gattactaat
     1441 aggtttgaat ctaatagttt atcagtttca tagtataagt tcattggtac ttctttaaat
     1501 tcaacgacaa cttgaattgt tttagatttc atacgtttac cagtacgaat atagaatggt
     1561 acaccagccc atctaaagtt atcaattgtt aatttacctg aaacaaaggt aggtgtgtta
     1621 gagtcatctg caacgcgatc ttcatcacgg tatgctttaa cttgtttacc atcgatatag
     1681 ccttcgccat attgaccacg aacaaagttc tttttaacat cttcagattg gaaatgacgc
     1741 agtgatttaa gtacttttac tttctcagca cggatatctt cactgtttaa actaataggt
     1801 gcttccatag ctaataatgc aaccatttgt aacatgtggt tttgcaccat atcttttagc
     1861 gcgccacttg attcataata accaccacga tcttcaacac ctagtatttc agaagatgta
     1921 acttggatgt ttgaaatata tttgttattc cataatggtt caaacatcgc attcgcaaaa
     1981 cgtaatacct cgatattttg aaccatgtct tttcctaaat agtggtcaat acgataaatt
     2041 tcttcttctt taaatgattt acgaatttga ttgtttaatg cttcggctga ttttaaatca
     2101 ctaccgaatg gtttttcgat aacaaggcgt ttaaatcctt ttgtatcagt aagaccagaa
     2161 gattttagat aatcagaaat aacgccaaag aattgtggtg ccattgctaa atagaatagt
     2221 cgattacctt ttaattcaaa ttggctatct aattcattac taaaatctag taattcttga
     2281 tagctttctt cattactaac atcatgtcta tgatagaaga catgttccat aaacgcgtca
     2341 attttgtttg tatcttttac gtgcttttga attgatgatt ttacttgatt acggaaatca
     2401 tcattagtaa tgtcacgacg tccaataccg atgatggcaa tatgttcatc taaattgtct
     2461 tgttggtaga gatggaatat tgatggaaac aacttacgat ggcttaagtc accagttgca
     2521 ccaaagattg tgattaaaca agggatgtgt ttgtttttag tactcaagat taaaacctca
     2581 attcttaatt agatatatga tttattatta taagataatc cattcagcag gtcattcaat
     2641 atgctcgaca gtatgcagtg tcttaaatga aatatcgatt catgtatcat gtttaatgtg
     2701 atacttatta atgataagta taacgtaatt gtcaaaattt atatagttat gtctacgtta
     2761 aagttagaaa aattaactag caaagacgaa tttttaacag attttgattc aagtataaat
     2821 taaaactaaa ttgatacaaa ttttatgata aaatgaattg aagaaaagga ggggcatata
     2881 tggaagttac attttttgga acgagtgcag gtttgcctac aaaagagaga aatacacaag
     2941 caatcgcctt aaatttagaa ccatattcca attccatatg gcttttcgac gttggtgaag
     3001 gtacacagca ccaaatttta catcatgcaa tcaaattagg aaaagtgaca catatattta
     3061 ttactcatat gcatggcgat catatttttg gtttgccagg attactttct agtcgttctt
     3121 ttcagggcgg tgaacagaag ccgcttacat tggttggacc aaaaggaatt aaagcatatg
     3181 tggaaatgtc tatgaattta tcagagtcac atttaaacta tccaattaca tatattgaaa
     3241 tcgatgatca tctgacatat catcatgatg gttttactgt agaggcgcat ttacttaatc
     3301 atggtattcc atcatatgga tatagagtaa tggcacctga aactacaggt acaatcaatg
     3361 ttgaagcgtt aaaaaacatt ggtcttgaac caggtcctaa atatcaagaa gtaaaatctc
     3421 atgatacatt tgaacataat ggtcaagttt atcaatcaaa agattttaga ggtgagtcca
     3481 aacagggtcc agttgtagca atttttggtg atacaaaacc atgttctaat gaacgtgtga
     3541 tttctagaga tgctgatgtg atggtacatg aagcgaccta tatagatggt gaaaagcatt
     3601 tagccaacaa ttatcatcat agccatattg aagacgtatt tgcattgata aaagaagcca
     3661 atgttaaacg tacgttgatt acacatttga gcaatcgcta taatacagaa gatataaatg
     3721 aaatttatca aatattgata caaaatgaag atacacctaa ttttaatttt gtaaaagatt
     3781 ttgatagttt taaagtctaa tgacaaagta ataaacaatg cttgccaaat agtaataatt
     3841 tataaaaata acccgtccaa ctatgtatgt gatgatgcat acatgtgttg gcgggtttgt
     3901 ttttattggt cttctacatt agaaagttca atactacggt cgacggcagc atttagacaa
     3961 tcttcgaaaa tagatactaa atcatattgt gacaatgtat caaggccagc ttgtgtcgta
     4021 ccacctttag aggtaatatt ttttcttaat tgagccatgc tcaaatctga acgttcaatc
     4081 atcttacttg tacctataat aaggttgcgt atagattctt caacttgttc tttttctaga
     4141 ccaagtttcg taccagcttt aacgtattgc tcgaatacat gatataaaaa tgctgggccg
     4201 cttccggtga tagctgttac ttgatgtaaa tgatcctctg atacttcaat tacagaacca
     4261 aatgctttaa ctaaatcgtt aatttcatct ttagatttag ggtcaaagtt gtttgaaaaa
     4321 ctaatgccag taactgagtg tccaacttgc gcatttgtgt ttggcataat tctagcaact
     4381 ggattttggc attctaattg ttgtttaata taatcaatcg gaatacctgc cataattgaa
     4441 atgaagcaat tgtcttttgt gatatgtggt ttgatgcgtg ttgctagagc atcaaagtca
     4501 tgcggtttgg tacctaaaaa aacataatct gcatctttta ataatgtcgc atcatcataa
     4561 ctatagttaa cacctagttt ttcagcgaat gcttttaaag cttgttcatt agatttattt
     4621 gttaaatata tatcattggc atctaagttg cttgagttaa taattcctgt aaatatagct
     4681 tgtgccatat taccagctcc ataaaatacg agtttcatta acgtcacatc cctttagttt
     4741 ttgatttgta tgttatattt tcttattatt gtatatatat taggaaagat aatcaacttg
     4801 tctcaattat acagagacga taaattgcat tctatataat tgaaaaggaa ggttaattga
     4861 tgaaaggaca acattttata gtgactggtg gtacgagtgg tttaggttta tcgattgtcc
     4921 gaaagttact agaaaataaa gtgcatgtaa cgttgttggt gcgtgatgtt gataaagcaa
     4981 ctagaatctt tgaacaagaa ttggggaaaa ctataaatgt tattccttgt gacttaaatg
     5041 atttgaagtc gatacaggca ctgcaatttg aagacaatac atcattcgat ggattcattt
     5101 atagtgcagg tttaggttat ttcaaatcta tttcagacca tagtttttca gaaatgattg
     5161 aaacgtatca gcttaattta attagtttta atgttttata tactgtgttg agaccatatt
     5221 taacatctaa cgcacatatt gtaggtattt caagtcaagc tgcattttcc acacaagcaa
     5281 atgctgcaca ttatggggca tcgaaagcag ggttttatgc attaatgaat gctttaagac
     5341 tagagtcacc aaacttacat atcatgacag ttaatgttgg cccgattgat acaccttttc
     5401 atcaaaaagc cgacccatca atgaagtatg ctaaaaagat gggggaaatt atgcttgatg
     5461 ctaatcagct tgcagaagat atcatttatg gcattaaaac aaaacaattg gaaattaatc
     5521 ggcctaaatg gatgcatcat gcgttgaaaa tgtatcaaat tgcgcctaga ttttttgaac
     5581 gatgtttccc taaattattt aaaaataaag cataaatagg tgggatggca atgaactgtc
     5641 aatcggagag tgtggtaaga ctttgtgtac gatatgcgga acaactatca gtatttgaag
     5701 aatttacagt gctggatatt ttaggcgata tttctgtaga ccaaatttca gacggtacat
     5761 tatattatac ttgtgaaaaa ttcaaactac ttgtgttgca aggaaatgtg ctgggtgtac
     5821 aaataataac taataatgat gaatcaacat gcgaagtgaa atatagaaag atgttttaat
     5881 aaaattatta agagatgaag tggcagaagt ttgcttaagg caatgccgca aggcgaaatt
     5941 actaaatact gatgcgtgag gacaacgctt taatttctat cataagtgag taatcataat
     6001 cataaattat agcgaattgt acataaacaa cagcagtaag gcatttttat aatgaaacgc
     6061 ctcatgctgc tgtttttatt actgaaaatg acttctacac taaatgattg gtatattcca
     6121 aatcctttac acgagctctt gctgttttaa tctgatctaa accaacttta gtatgatagt
     6181 tttcaatatt ttcttttaat tggtcgacgc tacttgcacc tacaatgatg gaaccaagca
     6241 cgtcatgtga tgttaaataa ctaaatgtca atgcagataa attactttca atttctttaa
     6301 tagaggctat tgtttcaccc aattcatcat gagaataatc aaaaatacca tctttaaatt
     6361 tattgtcgag cacattaaca ctgtttgaag ttaataatcc tttagacaca ggtcctcttg
     6421 ccaatacttt aacatcattg ttgtgaattg catctaataa tgattctgga cgattatcaa
     6481 tcaaattgaa ttgagacatt atcgtttcga tttgactatg ttttaaataa taatcaatta
     6541 catttgggcg aatagaagaa ataccgtaag cacgtataat tccttcttgt ttcaattcat
     6601 caaatgcgct tattgtttcg tctaatgggt catcaatggt tccgccatga agttgatata
     6661 aatcgatatg atcgatacct aaacgcttta gtgaaccttt aactgcctct ttaatatagg
     6721 atttactcgg atcccatgtt gtactgccat cttttgttaa acgattgcct acttttgtac
     6781 caatgaaaat atcatcgcgt tgttgatatt taagaagtgc cttaccaaca actgattcat
     6841 ttataccttt atcgtacatg tctgctgtat caaaataagt gataccattt tcaacagcac
     6901 aatctataat ttgttcggct ttttttaaat ctgtacctaa actcatgcaa cctagaccta
     6961 attcagataa tgcaatacca ctttttaata tatttttttg catgtctgtt ctcctttcga
     7021 tacactttag ctaacaagca tagtgtatca atatataata tgaatttaaa ggaacgtgat
     7081 tataatggat ttaaatgaaa aaacaattga tcgaacagtt atttataatg gcaaaattgt
     7141 agatgtagaa attcatacag tgacattacc aaacggtgaa acgtcaacaa gagaattagt
     7201 ttatcataat ggtgcagttg ctgtttgtgc attgacacct aaaaaagaag tcgtattagt
     7261 gaaacagtat cgtaaaccag tagaaaaacc attgctagaa attccagctg gtaaattaga
     7321 agatgatgaa aatagagtcg aagcggctaa gcgtgaatta gaagaagaaa caggatatat
     7381 tgccaaagag ttaacacatg ttgtagatat gtatggttca ccaggttttt gcgatgaaca
     7441 attatcaatt tattttactg ataatttaga agaaggtaca gttcacttag atgaagatga
     7501 atttgtcgaa gtcattaaag ttccgattga gaatgttaaa tcgatgctaa tgaataaaga
     7561 aatagaagat gcgaagacta tcattgcatt gcaacaccta ttattaaatt ataatcattc
     7621 taaataacaa atgagaaaag cttgcatttt attgagaaaa ctgttagttt taattgtgaa
     7681 gtttgaaata atttgtaatg attttaatta ttagtagggg agtggacatc gttggaagaa
     7741 cgattaaatc gcgttaagca acaattacaa caatcatcat ataagctaac gccacaacgc
     7801 gaagctactg ttagagttct aattgaaaat gaaaaagatc atctaagtgc tgaagacgta
     7861 tatctgaaag taaaagacaa agcgcctgaa attggcttgg cgacagtata cagaacgtta
     7921 gagttgttag ctgaactaaa agttgtcgac aaaattaact ttggtgatgg cgtcgctcgt
     7981 tttgatttaa gaaaagaagg cgcaaaacat ttccaccatc atttagtatg tatggaatgt
     8041 ggtcgtgtag atgaaatcga tgaagatttg ttaccagaag ttgaaaatcg agttgaaaat
     8101 gagttcaatt ttaaaatttt agatcatcgt ttaactttcc atggtgtgtg tgaaacatgc
     8161 caagctaaag gtaaaggata gtaaattgcg taggttaaat taaccttcgc tttttttaga
     8221 ggtgtggtta tggaaacaat tattgaagaa tatttgcgtt ttatacaaat tgaaaaagga
     8281 ctaagttcta acacaattgg tgcttataga cgtgatttga aaaagtatca agattatatg
     8341 actgaacatc atatctcgca tattgatttt atagatcgac aattaattca agagtgtttg
     8401 gggcatttaa tagaccaagg gcaatctgct aaatctattg cgcgatttat ttcaacaatc
     8461 cgtagttttc atcaatttgc tataagagaa aaatatgcgg cgaaagatcc aacggtatta
     8521 ttagattcac caaaatatga caaaaaattg cctgacgttt taaatgttga cgaagtattg
     8581 gctttattag aaacgccaga tttaaataaa attaatggat atcgtgatcg tacgatgtta
     8641 gaacttctgt acgcaacggg aatgcgtgta tctgaattga tacatttaga gttagaaaac
     8701 gtgaacttaa taatgggatt tgtacgcgta tttggtaaag gcgataaaga aagaattgta
     8761 ccattaggcg acgcagtcat tgagtactta actacttata ttgaaacgat tagaccgcaa
     8821 cttttaaaaa agactgttac tgaagtctta tttttaaata tgcatggtaa acctttatca
     8881 cgacaagcaa tatggaaaat gattaaacaa aatggcgtaa aggcaaacat taaaaagacg
     8941 ttaacgccac atacgttacg ccactctttt gcgacacatt tattggaaaa tggcgcagat
     9001 ttaagagcag tgcaagagat gttaggtcac tctgacatat ctactaccca actctataca
     9061 catgtttcga aatctcaaat tagaaaaatg tataaccaat ttcatcctag agcataaagt
     9121 gaaccataac ttaaaagtca caatacacat gactaaaaat gtctgtgcta ttgtggcttt
     9181 tttaaattgg ttgattaatt acgtctatgt tttcttaatt gaatcgcttc ttcttttgct
     9241 gcaatcactt ctgaacgatc acgccgcatg tgatggtcta caataaaagg atctgttgct
     9301 gtttcctgat tataatcata gtctggatag ttggccttga tgatgcgttc aaagactgga
     9361 gttattggta atataacaga tgaaaaaggc tttgctgcat tcaattttgc aatctgttgc
     9421 tcaattaaca attgataatc atttaaatta aggtataacg catctctatc tttagcattt
     9481 tgtattattt ctttagattt attaaaagac ttataggcgc cttttaaatt attgcggcga
     9541 taatggtaac aagcagttgc aaacaagatt aaactaacaa ctgcatcttg cttactgtag
     9601 ttattttcag ctttccatgc atcttctaaa atgtcatgac ataggaaata atgttgctta
     9661 gtatgaaatt gataatagaa atttatcagt gcctgttgca ttttgttatc accccaattt
     9721 aaaagtaagt tattttcatg ctataatatt ttagagaatt atgcacatat gacgcaatac
     9781 gaggtagata ttatgtatga agttaaatta gatgctttca atggaccatt agatttattg
     9841 ctgcatctta tccaaaaatt tgaaatagat atttatgata ttcctatgca agcattaaca
     9901 gagcagtata tgcagtacgt tcatgcaatg aaacagcttg aaattaatat tgcaagtgaa
     9961 tacctagtat tagcgtcaga actcttaatg attaaaagta agatgctatt accacaatca
    10021 acatcagata tggatgttga tgatgaccca cgggaagatt tagttgggcg tttaatagaa
    10081 tatcaaaatt ataaagaata tactgctatt ttaaatgaca tgaaagaaga aagagatttt
    10141 tattttacaa aaagaccgac agatttatct catttggaaa cagatgaatc ttgggatcca
    10201 aatcatacga ttgatttaac tgaattaatt gtagcttatc aaagagttaa aaatagagtt
    10261 gagttaaata cacctaaatc tgttgaaatc cgaaaagaga catttaccat tcaacaagct
    10321 acagaacaag tgacatcgag attgaaagat aaagatcatt ttaacttctt tagtctgttt
    10381 acgttttctg agccaattga acaagtagtc actcactttt tagctatttt agagatgtca
    10441 aaagcaggaa taattaatat tgagcaacaa cgtaattttg aagatattaa cattattaga
    10501 ggagtgaact accattttgg ataatcatgg tatattagag tcgcttttat ttacagctgg
    10561 cgatgaaggt ttagatgaaa aacaactatt agaaatatta gatatgtcga aagaccaact
    10621 cgttgaatta attgaaaatt attcatcaca tggattaatg atacaacgat ttggaacgac
    10681 gtatgtttta acgactaaaa aagaagcggc aacgtatatt gaacaattaa ttgaacaaaa
    10741 gtcacaaatg aaattatcac aagcagcaat ggaagtacta tcaattattg cttataacca
    10801 gccattatca agaagtgata ttgaattaat tcgtagtatc aattcagatg gtgcagttaa
    10861 gacattgatt gccaaaggac tagttgaggc taaagtggtt aatgaacagc gtagccaaca
    10921 gttaattact actgatttat ttttaaatgt atttggtatt tccaatatag aagatttgcc
    10981 gacaactgaa gaagacgatg aagaaatgga tgcttttttc agtaatctag tcaatcaaaa
    11041 aggagaaaat aatgactaaa gaattagaac gattacaaaa acgtatagct aatagcggtt
    11101 atacttcaag acgtaaggca gaaactttaa tttctgaagg aaaagtgaaa gtgaatggta
    11161 cgactgtcac tgaattagga acaaaagtga agccatcaga tacgattgaa gttgaaggta
    11221 ttaaaataga actagaagac aaaatttaca ttttattcca taaaccaact caagtgataa
    11281 caagtgtatc tgatgataga ggacgtacag tggttacaga ttactttaaa gatatagaag
    11341 cacgtattta tcctgttggt cgtttagact atgatacttc tggattgtta ttacttacaa
    11401 atgatggtga atttactaat ttaatgacac atccaagata tcaaattaag aagaaatatg
    11461 tagcaaaatt aaaaggttac ttaatgagag aagaagtgaa agccctagaa aaaggtattg
    11521 aattagaaga tggtatgacg caaccggctg aagtgaaagt taagaaacaa gataaagata
    11581 aaaacacaac attggttgaa attacaatta cagaaggtcg taatcgccaa gtcagaagaa
    11641 tgttcgaaca ttttggtcat caagtgacga aactgtctcg tattgaatat gggcctttaa
    11701 atgttgtcgg tttgaatgct ggtgaaggac gtgtattgac gcctcatgaa gtaaaagtga
    11761 tgcgtcattt agcagaacat gggaaataat taaataaaat atgtatttat cacaaagttt
    11821 gagaattttt tcacaaaatt ttaggttttt aataactgta tgcgctttcc tgtgatataa
    11881 tacttttgta aagaaaagca tgtgtgggag gtatgacctg tatgtcgaac gaaatactta
    11941 tcgtagatga tgaggataga atcagaagat tacttaaaat gtatttagaa agagaatctt
    12001 ttgaaatcca tgaagcaagt aatggccaag aggcttatga acttgcaatg gagaataatt
    12061 atgcttgcat actactagat ttaatgttgc ctgaaatgga tggtatccag gtggcaacta
    12121 aattgcgtga acataaacaa acaccgatta ttatgttgac tgctaaaggt gaagaaacaa
    12181 accgtgttga aggttttgaa tctggtgcag atgattatat cgtcaaacca ttttcaccaa
    12241 gagaagtagt cttaagagtt aaagcacttc taagaagaac gcaatctaca actgtagaac
    12301 aaagcgaacc tcacgcacgt gatgtgattg aatttaaaca tttagaaata gataatgatg
    12361 cacatcgcgt acttgctgat aatcaagaag ttaatttgac tcctaaagag tacgaattat
    12421 taatatattt agctaaaaca ccaaataaag tatttgaccg tgaacaatta ttaaaagaag
    12481 tttggcatta tgaattctat ggtgatttaa gaacagttga tactcatgtt aaacgactta
    12541 gagaaaagtt aaatcgtgtg tctagcgaag ctgcgcatat gattcaaaca gtctggggcg
    12601 ttgggtataa atttgaggtt aaatctaatg atgagccggc taaatagtgt cgtaattaaa
    12661 ctgtggttaa ctattatttt aatagtgacg acagttttaa ttttattaag tattgcttta
    12721 attaccttta tgcaatacta tttcacacaa gaaaccgaaa atgccataag agaagatgct
    12781 agacgtataa gttcactggt cgaacaatca cataataaag aagaagcaat aaaatatagt
    12841 caaacattaa ttgaaaatcc tggtgggttg atgattataa ataataaaca tcgtcaatca
    12901 acggcttcac tttctaatat taaaaagcaa atgttgaatg aagtagtcaa caacgaccat
    12961 tttgacgatg tgtttgataa aggtaaatct gttactcgaa atgtaacgat taaagaaaag
    13021 ggttcatctc aaacatatat tttgttaggc tatccaacaa aagcacagaa gaatagtcat
    13081 agcaaatata gtggagtctt tatatataaa gacttgaaat caatcgaaga tacaaataat
    13141 gctattacga ttatcactat aattacggct gttattttct taacaattac aacagtcttt
    13201 gcgtttttct tatcgtcaag aattacaaaa cctttaagac gtttaagaga ccaagctaca
    13261 cgtgtatctg aaggggatta ctcttataaa ccttctgtca caacgaaaga tgaaattggt
    13321 caattatcgc aggcatttaa tcagatgagt acagaaatcg aagagcatgt cgacgcatta
    13381 tccacatcta aaaatattag agacagctta attaactcta tggtagaagg tgtcctaggt
    13441 attaatgaga gtcgacaaat tatcttatct aataagatgg cgaatgatat tatggacaat
    13501 attgatgaag atgctaaagc tttcttatta agacaaattg aagatacttt taaatcaaaa
    13561 caaacggaaa tgcgtgattt agaaatgaat gcacgattct ttgttgtgac cacaagctat
    13621 atcgataaga ttgaacaggg aggtaaaagt ggtgttgttg tgacagttcg tgatatgact
    13681 aatgagcaca atctagatca aatgaagaaa gatttcattg ctaatgtatc acatgaatta
    13741 cgaacaccta tatcattact acaaggttat actgaatcaa ttgtagatgg tattgttaca
    13801 gaaccggatg aaataaaaga atcgctcgcc gttgtccttg atgaatcgaa acgtttaaat
    13861 cgattagtta atgaattgtt aaatgtcgca cgcatggatg ctgaagggtt atccgtaaat
    13921 aaagaagttc agcctattgc agcgttacta gataagatga aaattaagta tcgccaacaa
    13981 gctgatgatt taggtctaaa tatgactttt aattattgta agaagcgtgt ttggagttat
    14041 gatatggatc gcatggacca agtactaacg aacttaattg ataatgcatc acgttatacg
    14101 aaacctggag atgaaattgc aattacttgt gatgaaaatg aaagcgaaga tattttatac
    14161 attaaagata caggcacagg cattgcacca gaacatttac aacaagtatt tgatcgtttt
    14221 tataaagttg atgcagcgag aacgcgaggt aaacaaggta ccggtttagg tttgttcatt
    14281 tgtaaaatga ttatcgaaga gcatggtggt tccattgatg ttaaaagcga attaggaaaa
    14341 ggcacaacat ttattattaa actaccaaaa ccagaataaa actgaatata gttatttaag
    14401 aacgcatgtt attgattaga gactctaatt tatagcatgc gttttttgat tg
//
LOCUS       DAOBBH010000049        14342 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.049, whole genome shotgun sequence.
ACCESSION   DAOBBH010000049 DAOBBH010000000
VERSION     DAOBBH010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14342
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.049"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            155..2434
                     /gene="secDF"
                     /locus_tag="P6L01_001113"
     CDS             155..2434
                     /gene="secDF"
                     /locus_tag="P6L01_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HDP3030194.1"
                     /translation="MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEV
                     LYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLAGVTDQN
                     EARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKK
                     VTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFK
                     GQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLF
                     MLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYE
                     RIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLL
                     GILMIFVTAVFLSRFLLSLLVSSNIFKNQFWLFGVKKNKRHDINEGVDVHDLKTSFEK
                     WNFVKLAKPLIGVSILIVVVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEQV
                     VKDSGLKADQIQINGKDNKVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPII
                     GQELAKNAMLALIYASIGIIIYVSLRFEWRMGLSSVLALLHDVFIIVAIFSLFRIEVD
                     LTFIAAVLTIVGYSINDTIVTFDRVRENLQKVKVITTTEQIDDIVNRSIRQTMTRSIN
                     TVLTVIVVVVAILFFGAPTIFNFTLALFIGLISGVFSSIFIAVPLWGIMKKRQLKKSP
                     KHKLVVYKEKKSNDEKILV"
     gene            2637..4910
                     /gene="recJ"
                     /locus_tag="P6L01_001114"
     CDS             2637..4910
                     /gene="recJ"
                     /locus_tag="P6L01_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDP3030195.1"
                     /translation="MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQ
                     AIESIISDTDVNHDALQLSDMTKAIERIKRAIANDEKILVYGDYDADGVTSTTILVTT
                     LQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
                     VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFK
                     ALVAIGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFI
                     IGPRLNAVGRLDDASLACELLMTDDEEEAAFLAEQVEHFNRERKDIVATITEEAMAMA
                     ETKVKKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSID
                     QVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVK
                     PVDVLLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTL
                     GESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQLIIQDIAMNEQQILD
                     YRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDL
                     SNSLQQLQFSQLYIVLQHNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCQH
                     LSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRSIDSSKVYQLRQQRMDVEKQL
                     LYQDFSEIKNWIKSQLS"
     gene            4932..5450
                     /locus_tag="P6L01_001115"
     CDS             4932..5450
                     /locus_tag="P6L01_001115"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDP3030196.1"
                     /translation="MDLKQYVSEVQDWPKPGVSFKDITTIMDNGEAYGYATDKIVEYA
                     KDRDVDIVVGPEARGFIIGCPVAYSMGIGFAPVRKEGKLPREVIRYEYDLEYGTNVLT
                     MHKDAIKPGQRVLITDDLLATGGTIEAAIKLVEKLGGIVVGIAFIIELKYLNGIEKIK
                     DYDVMSLISYDE"
     gene            5878..8067
                     /locus_tag="P6L01_001116"
     CDS             5878..8067
                     /locus_tag="P6L01_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDP3030197.1"
                     /translation="MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFR
                     KNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIV
                     DGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQ
                     IRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYI
                     ETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSIN
                     DCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHE
                     IAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQS
                     DKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIV
                     EIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEVEIKEQGFR
                     VEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNE
                     AQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHR
                     TDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLI
                     KVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN"
     gene            8079..8531
                     /locus_tag="P6L01_001117"
     CDS             8079..8531
                     /locus_tag="P6L01_001117"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDP3030198.1"
                     /translation="MKVVVQRVKEASVTNDTLNNQIKKGYCLLVGIGQNSTEQDADVI
                     AKKIANARLFEDDNNKLNFNIQQMNGEILSVSQFTLYADVKKGNRPGFSNSKNPDQAV
                     KIYEYFNDALRAYGLTVKTGEFGTHMNVSINNDGPVTIIYESQDGKIQ"
     gene            8528..9403
                     /locus_tag="P6L01_001118"
     CDS             8528..9403
                     /locus_tag="P6L01_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDP3030199.1"
                     /translation="MKKIEAWLSKKGLKNKRTLIVVIAFVLFIIFLFLLLNSNSEDSG
                     NITITENAELRTGPNAAYPVIYKVEKGDHFKKIGKVGKWIEVEDTSSNEKGWIAGWHT
                     NLDIVADNTKEKNPLQGKTIVLDPGHGGSDQGASSNTKYKSLEKDYTLKTAKELQRTL
                     EKEGATVKMTRTDDTYVSLENRDIKGDAYLSIHNDALESSNANGMTVYWYHDNQRALA
                     DTLDATIQKKGLLSNRGSRQENYQVLRQTKVPAVLLELGYISNPTDETMIKDQLHRQI
                     LEQAIVDGLKIYFSA"
     gene            9864..11126
                     /locus_tag="P6L01_001119"
     CDS             9864..11126
                     /locus_tag="P6L01_001119"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDP3030200.1"
                     /translation="MIKIPRGTQDILPEDSKKWRYIENQLDELMTFYNYKEIRTPIFE
                     STDLFARGVGDSTDVVQKEMYTFKDKGDRSITLRPEGTAAVVRSYIEHKMQGNPNQPI
                     KLYYNGPMFRYERKQKGRYRQFNQFGVEAIGAENPSVDAEVLAMVMHIYQSFGLKHLK
                     LVINSVGDMASRKEYNEALVKHFEPVIHEFCSDCQSRLHTNPMRILDCKVDRDKEAIK
                     TAPRITDFLNEESKAYYEQVKAYLDDLGIPYIEDPNLVRGLDYYTHTAFELMMDNPNY
                     DGAITTLCGGGRYNGLLELLDGPSETGIGFALSIERLLLALEEEGIELDIEENLDLFI
                     VTMGDQADRYAVKLLNHLRHNGIKADKDYLQRKIKGQMKQADRLGAKFTIVIGDQELE
                     NNKIDVKNMTTGESETIELDALVEYFKK"
     gene            11142..12908
                     /gene="aspS"
                     /locus_tag="P6L01_001120"
     CDS             11142..12908
                     /gene="aspS"
                     /locus_tag="P6L01_001120"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDP3030201.1"
                     /translation="MSKRTTYCGLVTEAFLGQEITLKGWVNNRRDLGGLIFVDLRDRE
                     GIVQVVFNPAFSEEALKIAETVRSEYVVEVQGTVTKRDPETVNPKIKTGQVEVQVTNI
                     KVINKSETPPFSINEENVNVDENIRLKYRYLDLRRQELAQTFKMRHQITRSIRQYLDD
                     EGFFDIETPVLTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMISGFDKYYQI
                     VKCFRDEDLRADRQPEFTQVDIEMSFVDQEDVMQMGEEMLKKVVKEVKGVEINGAFPR
                     MTYKEAMRRYGSDKPDTRFEMELIDVSQLGRDMDFKVFKDTVENDGEIKAIVAKGAAE
                     QYTRKDMDALTEFVNIYGAKGLAWVKVVEDGLTGPIGRFFETENVETLLTLTGAEAGD
                     LVMFVADKPNVVAQSLGALRVKLAKELGLIDETKLNFLWVTDWPLLEYDEDAKRYVAA
                     HHPFTSPKEADIAKLGTAPEEAEANAYDIVLNGYELGGGSIRIHDGELQEKMFEVLGF
                     TKEQAQEQFGFLLDAFKYGAPPHGGIALGLDRLVMLLTNRTNLRDTIAFPKTASATCL
                     LTNAPGEVSDKQLEELSLRIRH"
     gene            12986..13176
                     /gene="ssrS"
                     /locus_tag="P6L01_001121"
     ncRNA           12986..13176
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="P6L01_001121"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            13241..13369
                     /locus_tag="P6L01_001122"
     CDS             13241..13369
                     /locus_tag="P6L01_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030202.1"
                     /translation="MGFIAFNKCFLVEFWENNQSDNAKYKILSYYVLMYKSVKEVK"
     gene            13369..14142
                     /locus_tag="P6L01_001123"
     CDS             13369..14142
                     /locus_tag="P6L01_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HDP3030203.1"
                     /translation="MKHQFSRNELAIGQQGLDLLKQQTVVILGVGGVGSFAAEALART
                     NIGHIILIDKDDVDITNVNRQLHALTTTVGQSKVSLMEERIKLINPDCKVTPLHMFYT
                     EETYEEIFDNYDIDYFVDASDTIMYKVHLMKECLDRGIKVISSMGAANKTDPTRFTIS
                     DISKTHTDPMAKIIRQKLKKLGIRKGIPVVFSDESPIVIREDVKDIVGDKNAINRKGQ
                     MPPSSNAFVPSVVGLICASYVVNDVLKDIPVRRIKDKGQ"
BASE COUNT         5238 a         1944 c         2752 g         4408 t
ORIGIN      
        1 aaatttaaat taaatatatc tatgactatt tttactccct gctctagtat gttaatatgt
       61 ataaggctaa taaattttgt aagaaagatt taacttgcgt tataaaatag tattcaagta
      121 ttctaaatac atacgtaaat aatgaggtgt tcatgtgaag aaaagtagta gaataattgc
      181 gttcttgctt ttagtcgtat tgttgtttgc cggaatggct gcaacttata aaagtgttat
      241 aaagaatgtc aatttaggac ttgatttgca aggtggattt gaggtgcttt atcaagtcga
      301 tcctttaaat aaaggcgaca aaatagataa aaaagcctta caatcaacag cgcaaacatt
      361 agaaaatcgt gttaacgttt taggtgtttc ggaacctaaa attcaagttg aagaacctaa
      421 tagaattaga gttcaacttg ctggtgtaac agaccagaat gaagcacgta aaattttatc
      481 ttctcaagct aatttaacaa ttcgcgatgc agaagataaa gtaaaattaa gcggttctga
      541 tattaaacag ggatctgcca aacaagaatt taaacaggaa acaaaccagc ctacagttac
      601 ttttaaagta aaagacaaaa acaaatttaa aaaagtaacg gaagaaatct ctaagaaacg
      661 tgataatgtc atggttgttt ggttagattt caaaaaaggt gacagctata aaaaagaagc
      721 acaaaagaaa aaccctaaat ttatttctgc agcaagtgtt gatcaaccaa tcaattctga
      781 tagcgtagaa atctcgggtg gatttaaagg acaagaaggc gttaaaaaag caaaacaaat
      841 cgccgaattg ttaaatgcag gttctttacc agttgacttg aaagaaattt attcaaactc
      901 agtaggtgcc caatttggtc aagatgcatt agataaaact gtgtttgcat cgtttattgg
      961 tgtagcatta atttatttat tcatgcttgg attctaccga ttacctggtt tagtagcgat
     1021 tattgcattg acaacttata tctatctaac gttagtagca tttaatttca tttccggggt
     1081 tctaacttta ccaggattag cggcgttggt attaggtgta ggtatggctg tagatgccaa
     1141 tattatcatg tatgagcgta ttaaggatga acttcgaata ggtagaacga taaagcaagc
     1201 cttttctaaa gcaaacaaaa gttcattcct aacaattttt gattctaact taacaacagt
     1261 tatcgccgca gcagtattat tcttcttcgg tgaaagttca gttaaaggtt tcgcgacaat
     1321 gttattatta ggtattctaa tgatctttgt tacagccgtg ttcttatcaa gattcttatt
     1381 atcattactt gtttcatcaa atatattcaa aaatcaattt tggttatttg gtgttaaaaa
     1441 gaataaacga catgatatta atgaaggtgt agatgttcat gaccttaaaa cttcattcga
     1501 gaaatggaat tttgttaaat tagctaagcc attaattgga gtaagtattt taattgtagt
     1561 tgtcggttta gttattcttt atatcttcaa gttaaactta ggaattgatt tctcaagtgg
     1621 tacacgtgta gatttccaat ctaaacaagc aattactcaa caaaaggttg agcaagttgt
     1681 aaaagatagt ggacttaaag cggatcaaat tcaaatcaat ggtaaagata ataaagtagc
     1741 aactgttcaa tttaaagacg atttaacgcg tgctcaagat aataaattga gtgataatat
     1801 taaatcgaaa ttcggtgaca cacctcaaat taatactgta tcaccaatta ttggacagga
     1861 attagcaaaa aatgcaatgc ttgcattaat ctatgcatcg ataggtatca tcatctatgt
     1921 atcattacga tttgaatggc gcatgggtct ttcatctgta ttggcattat tacatgatgt
     1981 attcattata gtagcgattt tcagtttatt tagaattgaa gtagatttaa catttatcgc
     2041 cgctgtatta acaattgtcg gttattcaat taatgataca atcgtaacgt ttgaccgtgt
     2101 acgtgaaaac ttacaaaagg ttaaagtgat tacgacaaca gaacaaattg atgatatcgt
     2161 taatagatca attagacaga caatgacacg ttcaattaat acagtattaa cagttattgt
     2221 agtagtagtt gctatactat tcttcggtgc tcctacgata ttcaacttta ctttagcatt
     2281 atttatcgga ttgatttctg gtgtattctc ttcaatcttc attgccgttc cgctatgggg
     2341 aataatgaaa aaacgtcagt tgaaaaaatc gccgaaacac aaattagttg tatataaaga
     2401 aaagaaatcg aacgatgaaa agattttagt ttaaaatgaa ttaagcggta tgtgaaacaa
     2461 taaagagttc acataccgct ttttacttta tttaaattta acaaaatata tataaataag
     2521 ttcccgcatt catttttaaa aaatggtata attaaagtct tgattattta gataagtgct
     2581 ttttatttaa agtaatttta gatataatgt cttttggaaa tgaggcgtaa atatatatga
     2641 ttaaaccgaa gtataagtgg aaattaacta aaccagctga atacataagt gatgaattaa
     2701 catcaaaatt aaagttaacg ccaatcgtaa aaaaaatatt agaaagtaaa tcgattattg
     2761 atgaacaagc aattgaatcc atcatttcag acacagatgt caatcatgat gcattgcaat
     2821 tgagcgatat gactaaggct attgaaagaa ttaaaagagc gatagcgaat gatgagaaaa
     2881 tcctagttta tggtgattac gatgccgacg gtgttacctc aactacaatt ttagtgacaa
     2941 cattacaact tttaggtgca caagtaggat ggcatattcc taaccgcttt accgaaggat
     3001 atggaccaaa cgaactagca tttagaaatg ctcatgatga aggcataaca ttaatcataa
     3061 ctgttgataa tggcatacaa ggtcataatg aaattaaaat ggttcaagat ttaggagttg
     3121 atgtcattgt aactgatcat catgaaattg gtagtacatt accagaagca tatgcaatcg
     3181 tacatccaat gcatccatcg tttaattatc cgttccaaca attgtgtggt gcaggtgttg
     3241 catacaaatt agcacaagca ttgattgaaa atgtacctga ttattttaaa gcattagttg
     3301 cgataggtac cattgcggat ttagtatcct taactgatga aaatcgatca ttagtgaaac
     3361 aaggattaaa agtcttaaat gatcaatgtc cgacctctgt taaagcattg ctaaaagaag
     3421 cgggatacaa tgacaatatt gatgaagaaa caattggttt tattattgga cctagactta
     3481 atgctgtagg tcgtttagat gacgcatcat tagcttgcga attgttgatg actgacgacg
     3541 aagaagaagc tgcattttta gcagaacaag ttgaacattt taaccgtgaa agaaaagata
     3601 ttgtagcaac tatcacagaa gaagcaatgg ctatggcaga aacgaaagtt aaaaagggag
     3661 atttattttt acttttagca aaagaaaatt ggcatgaagg tgtcttaggt attgttgctt
     3721 cgaaaattgt cgaaacattt gctttgccaa cattaatttt aaatattgat agggagcaaa
     3781 atcatgcaaa aggttccgcc agatcaattg atcaagtgtc gatgtttgaa atactatcag
     3841 cacatcaaga gttaatcgct aaatttggcg gccatcatat ggctgcaggt atgacgatgg
     3901 atattgaaaa tatagaatcg ttagctgaag gtttaaataa atggatgaaa gaattatcta
     3961 aaacaacttc gttagatccc gtaaagccag tagacgtatt attaacagaa aatgatataa
     4021 cgattaaaaa tattcgtgat atgaatcgtt taagaccctt cggaacagat ttttctagac
     4081 ctatttttga aatggatgat ttatcagttt cttcagttaa ggcgatcggt caacaaaaaa
     4141 atcatctaaa attgacactt ggtgaaagta atatcgccgc gctattttgg caaaatggac
     4201 acttagaacc tgagttacaa gatgaacagc caattaatat attaggatct gttcaaatca
     4261 acgaatggaa tggtaatcaa tcaccgcaat taattattca agatattgcg atgaatgaac
     4321 agcaaatatt agattataga agtaagcgaa aaagtttacc ttttacagaa aatgatgaaa
     4381 atattgtcgt gcttattcat cctaaaagtg ataaagtaaa tgcgaatgaa tattattatg
     4441 gtgaagaaat taaacaacaa actgataaag tagtattaag agatttacca acgtcaatgg
     4501 aagacttgtc taattccttg caacaactgc aattttctca actttatata gttttgcaac
     4561 ataatcattc gatttacttc gatggtatac ctaatatgga tatttttaaa aagtgttata
     4621 aagcattaat aactaaacaa gaaacaaata tccagaaaga gggtatgtta ttgtgtcaac
     4681 atttaagtgt gaaaccagat acacttaaat tcatgttgaa agttttctta gacttaaaat
     4741 ttgtaacaca agaagatggt ttaattcgaa tcaatcaaca acctgataaa agatcgattg
     4801 attccagcaa agtatatcaa ttaagacaac aacgtatgga tgttgaaaag caattattat
     4861 atcaagattt ttcagaaata aaaaattgga taaagtcaca attgtcgtga gcaatttagg
     4921 aggaaatatt aatggattta aagcaatacg tatcagaagt tcaagattgg ccgaaaccag
     4981 gtgttagttt caaggatatt actacaatta tggataatgg tgaagcatat ggctatgcaa
     5041 cagataaaat tgtagaatac gcaaaagaca gagatgttga tatcgttgta ggacctgaag
     5101 cgcgtggctt tatcattggc tgtcctgtag cttattcaat ggggattggc tttgcacctg
     5161 ttagaaaaga agggaaatta cctcgtgaag tcattcgtta tgagtatgac ctagaatatg
     5221 gtacaaatgt tttaacaatg cacaaagatg caattaaacc aggtcaacgt gtgttaatta
     5281 cagatgattt attagctact ggtggtacga ttgaagcagc aataaaatta gttgaaaaat
     5341 taggcggtat cgtagtaggt attgcattta taattgaatt gaaatattta aatggtattg
     5401 aaaaaattaa agattacgat gttatgagtt taatctcata cgacgaataa taaataatat
     5461 aattttatca aatgaaatcc ttcatcaaat gtataagaac caatgactta attaaaaaag
     5521 ttgtttaagt tttcttaaca tgagatgtta ggatttttta tttactgaaa atgttagatg
     5581 attgagcatt ataccttaat aacatcgttt atttatttca taaattgtag tatcatagaa
     5641 ctaatattta aaaaatgaaa cagtagattt aggtcgaatt tttgtaaaag ttttaaaagt
     5701 aggaatagta tacaaattaa actcgctcaa gtaaaattaa tattacgatt aatgacgaca
     5761 ggataaatat ttatcgtcga cggacgtatg attggtgtgg gacaaatact attcaacaag
     5821 agtacctaaa tcattgttta aggcgaagta ataaatatga atggggtgta tcatataatg
     5881 aacaacgaat atccatatag tgcagatgaa gttcttcaca aagcaaaatc atatttgtca
     5941 gcagatgaat atgagtatgt tttaaaaagc tatcatattg cttatgaagc acataaaggt
     6001 cagttccgaa aaaacggatt accatacatt atgcatccta tacaagttgc aggtatttta
     6061 acagaaatgc gattagacgg accgacgatt gtcgcaggtt ttttgcatga tgtaattgaa
     6121 gatacaccgt atacatttga agatgtaaaa gaaatgttca atgaagaagt tgctcgaatt
     6181 gttgatggtg tgacgaagct taaaaaagta aaataccgct caaaagaaga acaacaagct
     6241 gaaaatcatc gcaagttatt tattgcgatt gccaaagatg tacgcgtaat tttggtgaaa
     6301 ttagcagaca gattacataa tatgcgtacc ttgaaagcca tgccgcgtga aaaacaaatt
     6361 agaatttctc gagaaacatt agaaatttat gcacctttag cacatcgtct tggtattaat
     6421 acaattaaat gggaactaga agatacggct cttcgttata ttgataatgt gcaatatttt
     6481 agaatagtca atttaatgaa gaagaaacgt agtgaacgtg aagcgtatat cgaaacggct
     6541 attgatagaa tacgtactga aatggaccga atgaatatcg aaggcgatat aaatggtaga
     6601 cctaaacata tttacagtat ttatcggaaa atgatgaagc agaaaaaaca atttgatcaa
     6661 atttttgatt tgttggcgat acgtgttatt gtcaattcta ttaatgattg ttatgcgata
     6721 cttgggttgg tgcatacgtt atggaaaccg atgccaggac gttttaaaga ttatattgca
     6781 atgcctaaac aaaatttgta tcagtcattg catactacag tagtaggtcc aaatggagac
     6841 ccgctcgaaa tccaaatacg aacgtttgat atgcacgaaa ttgctgagca tggtgttgca
     6901 gcacactggg cttacaaaga aggtaaaaaa gtaagtgaaa aagatcaaac ttatcaaaat
     6961 aagttaaatt ggttaaaaga attagctgaa gcggatcata catcgtctga cgctcaagaa
     7021 tttatggaaa ccttaaaata tgacttacag agtgacaaag tatacgcatt taccccagcg
     7081 agtgatgtta ttgagttgcc atatggtgct gtgccgattg attttgctta tgcgattcac
     7141 agtgaagtag gtaataagat gattggtgcc aaggtgaatg gcaaaattgt accaattgac
     7201 tatattttac aaacaggcga tattgttgaa atacgtacta gtaaacattc atatggacca
     7261 agtcgtgatt ggttgaaaat tgttaaatcg tctagtgcca aaggtaaaat taaaagtttc
     7321 ttcaaaaaac aagatcgttc atctaatatt gaaaaaggcc gaatgatggt tgaagttgaa
     7381 ataaaagagc aaggatttag agtcgaagat attttgacag agaaaaatat tcaggttgtt
     7441 aatgaaaaat ataactttgc aaatgaagat gatttattcg cagctgtagg atttggcggc
     7501 gtgacatcct tacagattgt taataaatta actgaaagac aacgtatttt agataaacaa
     7561 cgtgctttaa atgaagcaca agaagttacg aaatcattgc ctattaaaga caacatcatt
     7621 actgatagtg gtgtctatgt agaaggttta gaaaatgtac ttatcaagtt gtcaaaatgt
     7681 tgtaatccta taccaggtga tgatattgta ggttatatca ccaaaggtca cggtattaaa
     7741 gtacatcgca ctgattgccc aaatattaag aacgaaactg aacgactaat taatgttgaa
     7801 tgggtaaaat caaaagacgc aactcaaaaa tatcaggttg atttagaggt aactgcgtat
     7861 gaccgaaatg gcttgttgaa tgaagtacta caagctgtta gctcgacagc cggcaattta
     7921 attaaagttt caggacgttc agatattgat aaaaatgcaa taataaatat tagtgtcatg
     7981 gtgaaaaacg tgaatgatgt ttatcgtgtg gtagaaaaga tcaaacaact tggtgatgtt
     8041 tatacagtaa caagagtttg gaactagagg tgcaaaatat gaaagtagtt gtacaaagag
     8101 ttaaagaagc atcggtgacg aatgatacat taaataatca aatcaaaaaa ggatattgtt
     8161 tattagtcgg tatcggtcag aactctacag agcaagatgc agatgtaatt gcaaagaaaa
     8221 ttgctaatgc aagattattt gaagatgaca ataataaatt aaactttaat atccaacaaa
     8281 tgaatggtga aatactatca gtttcacaat ttactctcta tgcagatgta aaaaaaggta
     8341 accgtccagg tttctcaaat tctaaaaatc ctgatcaagc ggtaaaaatt tatgagtatt
     8401 ttaatgatgc gctacgagcg tatggtctta ctgtgaaaac aggtgaattt ggaacacaca
     8461 tgaatgttag cataaataat gatggtccag tcactattat ttatgaaagt caggacggca
     8521 aaattcaatg aaaaaaatag aggcatggtt atctaaaaag ggtcttaaaa ataaacgtac
     8581 tctaatagta gtgattgcct ttgtcttatt tatcatcttt ttatttttat tgctgaatag
     8641 caatagtgaa gatagtggga acatcacgat aactgaaaat gctgaattac gtacaggtcc
     8701 aaacgctgcg tatccagtca tatataaagt tgagaaaggt gaccatttta aaaagattgg
     8761 taaagtaggt aaatggattg aagttgaaga tacatccagt aatgaaaaag gttggatagc
     8821 tggatggcac acaaatttag atattgtcgc ggataatacg aaggagaaaa atcctttgca
     8881 aggtaaaaca atagtgcttg atcctggtca tggaggtagt gaccagggtg cttcaagcaa
     8941 tactaaatat aaaagtttag aaaaagacta tacgttgaaa acagcaaaag aattgcagcg
     9001 tactttagaa aaagaaggcg caactgttaa gatgacaaga acagacgata catatgtttc
     9061 actagaaaat cgtgatatca aaggcgatgc ctatttgagt atacataatg atgcattaga
     9121 atcatctaat gcaaatggaa tgacagttta ttggtatcat gataatcaaa gagctttagc
     9181 agatacgtta gacgctacga ttcagaagaa aggtttactt tctaatcgcg gttcaagaca
     9241 agaaaattat caagtgttaa gacaaacaaa agttcctgct gttttattag aattaggtta
     9301 tattagtaac ccaactgatg aaacgatgat taaagatcaa ttacatagac aaattttaga
     9361 acaagcaatt gttgatggac ttaaaattta tttttctgcg tagggcttgc aaaaatatgt
     9421 gaaagtagtt atcattgata ttgaatttta taactaaaac cgttagtatt cttgaaatgg
     9481 taaatgaaat aggtagcaat ctaactaaga ttgtgtagga atataatcca tagactgaaa
     9541 gattatgctg agtagtttat atacattgaa cacaagaaga ggtgctttat gaaaagtaaa
     9601 gccgttaaac gtacgttaaa cgttttgagt gggtttattt aatgcacgct tataaaaagt
     9661 aatgatgatt acaattaggc atgtttttta aaccaatgag ggtggcaaca cggatattcg
     9721 tcccttgtgc agtttcaggc tgtgcaaggg ctttttcttt tgcttttaaa attatgattt
     9781 atcatgcaaa gttaagtgat cgtatatagt tttagtttta aaaaggtaat taaataaaat
     9841 agtttgccga gggagatgtc aaaatgatta aaatacctag agggacgcag gatattttac
     9901 ctgaagattc aaagaaatgg cgttacattg aaaatcaatt agatgaatta atgacatttt
     9961 ataattataa agaaataaga acaccaattt ttgaaagtac agatcttttt gcaagaggtg
    10021 ttggtgattc aaccgatgtc gtacaaaaag aaatgtatac atttaaagat aaaggcgaca
    10081 gaagtattac attaagacct gagggaacag ctgcagttgt gcgttcatat attgaacata
    10141 aaatgcaagg taatccaaac caaccaatta aactttatta caatggaccg atgtttagat
    10201 atgaacgtaa gcaaaaaggg cgctatcgtc aatttaatca atttggtgta gaagctattg
    10261 gtgctgaaaa tcctagcgta gatgcagaag tattagctat ggttatgcat atttatcaat
    10321 catttggatt aaaacattta aagcttgtta ttaatagtgt aggggatatg gcgtctcgaa
    10381 aagaatataa cgaagcgtta gtgaaacact ttgaaccagt aattcatgaa ttttgttcag
    10441 attgtcaatc acgtttgcat acaaatccga tgcgaatttt ggattgtaaa gttgaccgtg
    10501 ataaagaagc gattaagact gcacctagaa tcactgattt cttaaatgag gaatctaagg
    10561 catattatga acaagtaaaa gcttatttag atgatttagg tattccatat attgaagatc
    10621 ctaacttagt tcgtggattg gattattata cacatacagc atttgaatta atgatggata
    10681 atcctaacta tgatggtgcc attacaacgc tttgtggtgg tggccgttat aatggtttat
    10741 tagaattgct agatggtcca agtgaaacag gtattggttt tgcgctaagt atagaacgat
    10801 tattgcttgc acttgaagaa gaaggtatcg aattagatat tgaagaaaac ttagatttat
    10861 tcattgttac aatgggtgat caagcagatc gatatgctgt gaagttatta aatcatttga
    10921 gacataatgg tattaaagca gataaagact acttacagcg taaaattaaa ggacaaatga
    10981 aacaagcaga ccgtttaggt gccaagttta caatcgttat tggtgatcaa gaattagaaa
    11041 ataataaaat cgatgttaaa aatatgacaa ctggtgaatc tgaaacaatt gaattagacg
    11101 cattagtcga atattttaag aagtagagag ggcgttaaaa tatgagtaag agaacaactt
    11161 attgtggatt agttactgaa gcatttttag gacaagaaat tacattaaaa ggatgggtta
    11221 acaatcgtcg tgacctaggt ggattgattt tcgttgattt aagagataga gaaggaattg
    11281 tacaagtcgt gtttaatcct gcattttcag aagaggcatt gaaaattgct gaaacagtac
    11341 gttctgaata tgttgtagaa gttcaaggta cagttacgaa gcgtgaccct gaaacagtta
    11401 atcctaaaat taaaactggc caagttgaag tacaagttac aaatattaaa gtgattaata
    11461 aatctgagac accaccattt tctataaatg aagaaaatgt taacgttgat gaaaatattc
    11521 gattaaaata ccgttattta gatttacgtc gtcaagagtt agcgcaaaca tttaaaatga
    11581 gacatcaaat tacacgttct attcgtcaat atttggatga tgaagggttc tttgacatcg
    11641 aaacaccagt actaacgaag tcaacacctg agggtgcacg tgactattta gtaccatctc
    11701 gtgttcatga tggtgaattt tatgcattac cacaatcacc acaattattt aagcaattat
    11761 tgatgattag tggatttgac aaatactacc aaatcgtaaa atgcttccgt gacgaagatt
    11821 tacgtgcaga tcgtcaacct gaattcacac aagtcgatat tgaaatgagt tttgtagatc
    11881 aagaagatgt gatgcaaatg ggtgaagaaa tgcttaaaaa agttgttaaa gaagttaaag
    11941 gcgttgaaat taatggcgct ttcccacgca tgacatataa agaagcgatg cgtcgctatg
    12001 gttctgataa accagataca cgttttgaaa tggaattaat tgacgtttct caattaggac
    12061 gtgatatgga ctttaaagta tttaaagata ctgttgaaaa tgatggtgaa attaaagcaa
    12121 ttgtcgctaa aggtgcagct gaacaatata ctcgtaaaga tatggatgct ttaacagaat
    12181 ttgtaaacat ctatggtgct aaaggattag cgtgggttaa agttgtggaa gatggtttga
    12241 caggtccaat tggacgtttc tttgaaacag aaaatgttga aacattactt acattaactg
    12301 gtgctgaagc tggtgactta gtaatgtttg ttgcagacaa accaaatgtc gttgcacaaa
    12361 gtttaggtgc attacgtgtc aaattagcta aagaattagg tttaatcgat gaaacaaaat
    12421 taaacttctt atgggtgaca gattggccat tattagaata tgatgaagat gcgaaacgtt
    12481 acgttgcagc acatcatcca tttacatctc caaaagaagc tgatattgct aagcttggca
    12541 ctgcgccaga agaagctgag gcaaatgctt atgacatagt attaaatggt tatgaattag
    12601 gtggcggttc aatcagaatt catgatggtg agttacaaga aaaaatgttc gaagttcttg
    12661 gatttactaa agaacaagca caagaacagt tcggcttctt actagatgca tttaaatatg
    12721 gtgcaccacc acatggcggt attgcattag gattagaccg attagtcatg ttattgacta
    12781 atagaacaaa cttgagagat acaatcgcat tccctaaaac agcatctgct acatgtttat
    12841 taacgaatgc gcctggtgaa gtttctgata aacaattaga agaactttct ttgcgaattc
    12901 gtcactaaat tttaaaaaat caataaactt tggtttgtta taatttcatt aattttaatt
    12961 atgtggtaat ataacatcat aagatagtca tctgagttgt tcgtaagttt gctttttaat
    13021 ttgggcctaa cactctttga tcaagggagc ccaatgggtt ttcttgcagc gtacacgcct
    13081 tatatgaagg acgtacaaaa caagaaacag ggcacccacc tgtatataac aggccgaatg
    13141 atcaagctat tataactacg gcaatacgga ctctatcgat acgcaagact ttgtcttgcg
    13201 tatttttatg cgtttcttta agtttttaat aaagaattag ttgggtttta tagcgtttaa
    13261 taaatgtttc ctcgttgaat tttgggaaaa taatcaaagc gataatgcta aatataaaat
    13321 tttaagttac tatgtactta tgtataaatc agtgaaagag gtaaaataat gaaacatcaa
    13381 ttttcaagaa atgaactagc gattggacaa caaggtttag atttattgaa gcaacaaact
    13441 gttgtcatac ttggcgttgg aggtgttggt tcatttgctg ctgaagcatt agcaagaacg
    13501 aatattggtc atattatatt aatagataaa gatgatgttg atattacaaa tgtaaacaga
    13561 caattacacg ctttaactac aacggtagga caaagtaaag tttctttgat ggaagaacgt
    13621 attaaattaa ttaatccaga ttgtaaagtc acgcccttac atatgtttta tacagaagag
    13681 acatatgaag aaattttcga taactacgat atagattatt ttgtagatgc aagtgataca
    13741 ataatgtaca aagtacactt aatgaaagag tgtcttgacc gagggattaa agtgatttcc
    13801 agtatgggtg ctgcaaataa aacagatcca acacgtttta caatttcaga tatctctaaa
    13861 acacataccg atccaatggc caaaattatt cgtcaaaaat tgaaaaaact aggaattcgt
    13921 aaagggattc cagttgtatt ttcagatgaa agtccaattg tcataagaga agatgtaaaa
    13981 gatatagttg gagataaaaa tgctatcaat cgaaaaggac aaatgccacc ttcttcaaat
    14041 gcctttgtgc caagtgttgt tggattaatt tgtgctagtt atgtggtgaa tgacgtatta
    14101 aaagatattc cagttcgtcg cattaaagac aaagggcaat aattcatttt gaaagtcata
    14161 attttcaacg taaggcaagt gtaaccacac cataaaacta aaactgacta gttcgcaagc
    14221 atagtagaat atgcctcgtg tactagtcag ttttgatttg attatagcta aaatatcaaa
    14281 ggtaatcgat ttaatcttat ttttacggcc tttgtttaag taagttgtta tatatttctt
    14341 tg
//
LOCUS       DAOBBH010000050        14243 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.050, whole genome shotgun sequence.
ACCESSION   DAOBBH010000050 DAOBBH010000000
VERSION     DAOBBH010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14243
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.050"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(63..599)
                     /locus_tag="P6L01_001124"
     CDS             complement(63..599)
                     /locus_tag="P6L01_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDP3030204.1"
                     /translation="MTKTMIRLASEQDAEKLQQLMHEAFTPLRELGIDWPSVNANLDA
                     VKENIDKNTTFVMTIDDEIISTITVRYPWGSVKSISGYPFVWWFATNPEYEGKGYGSQ
                     LLTYVEEAFLRDTLKSAAVTLGTSARLHPWLLKIYEKRGYEIYSEHENDDGDLGVIMR
                     KVLIPERFDETILGQPPF"
     gene            complement(833..1657)
                     /locus_tag="P6L01_001125"
     CDS             complement(833..1657)
                     /locus_tag="P6L01_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDP3030205.1"
                     /translation="MIENKKTVEDTYSTGAIVDSISSSVQMKQVMVQQTPGRYMLKAM
                     MAGFLLSIVTVFMFGIKTQFANTHVDGLINLMGAIAFSLGLILVVLTNSELLTSNFMY
                     FTVGWYYKVVSVKKMTWILLYCFLGNILGGFVLFFLMKFAHVMTPEMTQALTALVQKK
                     TVDSTWLNIFTKGIFCNFFINIGIFISMQFKGGLTKAFFIACGVVVFVYMGYEHVVFN
                     AGLYAGMVFFNLDAVSWLHVLKNIVFAFLGNFVGGGIFVGLVYAFLNGKRNRLEQQ"
     gene            complement(1874..2056)
                     /locus_tag="P6L01_001126"
     CDS             complement(1874..2056)
                     /locus_tag="P6L01_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030206.1"
                     /translation="MVERYIKVLILYIFTTLLSSISVTSKCVPNKVIRFILRTAIGYS
                     IFAYGLHYFSNLKKNK"
     gene            complement(2140..2607)
                     /locus_tag="P6L01_001127"
     CDS             complement(2140..2607)
                     /locus_tag="P6L01_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HDP3030207.1"
                     /translation="MTIKVEDNKIIFSRTIEAPIEKVFDAYTTKELFEKWFHPKGAST
                     KVFRFNAVSGGDAFYAIKTPTMTSYTLAEYKTVKRPYLIEYIDSFATPQGAKDTKMPS
                     MKITLSFSKSNTTKTTVTSTSVFPTKEAAQQVINMGVEQGMNQTLDQLDALLK"
     gene            complement(2793..4340)
                     /gene="adcA"
                     /locus_tag="P6L01_001128"
     CDS             complement(2793..4340)
                     /gene="adcA"
                     /locus_tag="P6L01_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HDP3030208.1"
                     /translation="MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQS
                     FAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVAST
                     IKDKDKKRSLEDKLDKAKLLTDQHEHGEEHEHEGHDHEKEEHHHHGGYDPHVWLDPKI
                     NQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISHE
                     SIGYLADRYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTETI
                     RKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAESK
                     HDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKDL
                     KAYYDKGYKTDITNIDIKGNEITFTKDGKKHTGKYEYNGKKTLKYPKGNRGVRFMFKL
                     VDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQI
                     KEEMLAH"
     gene            complement(4529..4600)
                     /locus_tag="P6L01_001129"
                     /pseudo
     CDS             complement(4529..4600)
                     /locus_tag="P6L01_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4635..4901)
                     /locus_tag="P6L01_001130"
     CDS             complement(4635..4901)
                     /locus_tag="P6L01_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDP3030209.1"
                     /translation="MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALE
                     GIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIKIASCKYHY"
     gene            complement(4901..5152)
                     /locus_tag="P6L01_001131"
     CDS             complement(4901..5152)
                     /locus_tag="P6L01_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDP3030210.1"
                     /translation="MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESD
                     YNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKDIDV"
     gene            complement(5317..5418)
                     /locus_tag="P6L01_001132"
                     /pseudo
     CDS             complement(5317..5418)
                     /locus_tag="P6L01_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5425..6024)
                     /locus_tag="P6L01_001133"
     CDS             complement(5425..6024)
                     /locus_tag="P6L01_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDP3030211.1"
                     /translation="MTKKLLTLFIVSMLILTACGKKESATTSSKNGKPLVVIYGDYKC
                     PYCKELDEKVMPKLRKNYIDNHKVEYQFVNLAFLGKDSIVGSRASHAVLMYAPKSFLD
                     FQKQLFAAQQDENKEWLTKELLDKHIKQLHLDKETENKIIKDYKTKDSKSWKAAEKDK
                     KIAKDNHIKTTPTAFINGEKVEDPYDYESYEKLLKDKIK"
     gene            complement(6043..6405)
                     /locus_tag="P6L01_001134"
     CDS             complement(6043..6405)
                     /locus_tag="P6L01_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HDP3030212.1"
                     /translation="MKRFVATVLLLLVFISGCGNDKYVKEIDEAVKIQNQKQEQLAKK
                     GNGDRVDHFERKDANIYVYDKDKIIILAYKPLSNDDEVHYYAYDFSDKRVSYKQDFDS
                     RRYYQQHDADYHEENMTN"
     gene            6660..7910
                     /gene="fmhA"
                     /locus_tag="P6L01_001135"
     CDS             6660..7910
                     /gene="fmhA"
                     /locus_tag="P6L01_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhA"
                     /protein_id="HDP3030213.1"
                     /translation="MNFVTLTSDEFNAFTTKHFSHYTQSAIHYNHRVDLKGDVHLVGV
                     KDDNGQVIAGCLLTEARTLKFFKYFYTHRGPVMDYTNQSLVAFFFKALTSYLKKHNCL
                     YVLVDPYLIENLRNADGEIVKSYDNRAFVRTMDKLGYKHQGFPVGYDSMSQIRWLSVL
                     DLKDKTEDQLLKEMDYQTRRNIKKTYDIGVKTKTLTIDETQTFFDLFHMAEEKHGFKF
                     RELPYFEEMQKLYDDHAMLKLAYIDLNEYLKTLQLKQQQLTAELSGVEEALEESPNSK
                     KNKTKRTQLEQQLNSNKRKIDNTIEQIEQDGAVLNLASALFIYNEHEVYYLSSGSNPK
                     YNAYMGAYHLQWEMIKFAKAHHIDRYNFYGITGDYSESSEDYGVQQFKKGFNAHVEEY
                     IGDFIKPIKPLLYKVQTYLNHKRR"
     gene            complement(8007..8738)
                     /locus_tag="P6L01_001136"
     CDS             complement(8007..8738)
                     /locus_tag="P6L01_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDP3030214.1"
                     /translation="MIQLNNIHKSFNDVEVIKGIDLSVEQGEVVTLIGRSGSGKTTLL
                     RMINALEIPTEGTVYVNGKTYTSKDKKSQIEVRKQSGMVFQSYNLFPHKTALENVMEG
                     LITVKKLKKDEARGKSLELLEKVGLTHVKDQRPHALSGGQQQRVAIARALAMNPKVML
                     FDEPTSALDPELVNDVLKVIKDLANEGMTMVIVTHEMRFAKEVSNNIVFIHEGMIGEQ
                     GAPEEMFNRPKTEELRRFLNVINEE"
     gene            complement(8735..9454)
                     /locus_tag="P6L01_001137"
     CDS             complement(8735..9454)
                     /locus_tag="P6L01_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDP3030215.1"
                     /translation="MFLNLNSEQQHALDAAKQAFGPMLEGLVKYSIPITLVTFVLGLI
                     IALFTALMRISTSKILRSISRVYVSIIRGTPMIVQLFIIFYGIPELGRLLTNDADNQW
                     TLAPVVAAIIGLSLNVGAYASEIIRGGIISIPKGQTEAAYSIGMTYGQTIQRIILPQA
                     IRVSIPALGNTFLSLIKDTSLLGFILVAEMFRKAQEVASTTYEYLTIYVLVALMYWVV
                     CFIISIIQGIYESYIERGYRS"
     gene            complement(9435..10214)
                     /locus_tag="P6L01_001138"
     CDS             complement(9435..10214)
                     /locus_tag="P6L01_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDP3030216.1"
                     /translation="MKRLLFVMIAFVFILAACGNNSSKDKEANKDSKTINVGTEGTYA
                     PFSFHDKDGKLTGYDIDVIKAVAKEEGLKLKFNETSWDSMFAGLDAGRFDVIANQVGI
                     NPDREKKYKFSKPYTFSSAVLVIRENEKDIKDFDDVKGKKLAQTFTSNYGKLAKDKGA
                     DITKVDGFNQSMDLLLSKRVDGTFNDSLSYLDYKKQKPNAKIKAIKGNAEQSRSAFAF
                     SKKADDETVQKFNDGLKKIEENGELAKIGKKWFGQDVSKSK"
     gene            complement(10335..11774)
                     /gene="mdeA"
                     /locus_tag="P6L01_001139"
     CDS             complement(10335..11774)
                     /gene="mdeA"
                     /locus_tag="P6L01_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdeA"
                     /protein_id="HDP3030217.1"
                     /translation="MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQ
                     VQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVA
                     RIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYLN
                     WRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISE
                     KGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSM
                     IGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRKLALVGMA
                     IVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSM
                     TNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAG
                     FLFALLITVVSFFCSFMLQGKKKEVDSRQ"
     gene            11948..12052
                     /locus_tag="P6L01_001140"
     CDS             11948..12052
                     /locus_tag="P6L01_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDP3030218.1"
                     /translation="MKLDLQTARRNLNSPNIKTRKRALKIIKQHKRAK"
     gene            complement(12206..12892)
                     /locus_tag="P6L01_001141"
     CDS             complement(12206..12892)
                     /locus_tag="P6L01_001141"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDP3030219.1"
                     /translation="MPKLILCRHGQSEWNAKNLFTGWEDVNLSEQGINEATRAGEKVR
                     ENNIAIDVAFTSLLTRALDTTHYILTESKQQWIPVYKSWRLNERHYGGLQGLNKDDAR
                     KEFGEEQVHIWRRSYDVKPPAETEEQREAYLADRRYNHLDKRMMPYSESLKDTLVRVI
                     PFWTDHISQYLLDGQTVLVSAHGNSIRALIKYLEDVSDEDIINYEIKTGAPLVYELTD
                     DLEVIDKYYL"
     gene            complement(13219..14085)
                     /locus_tag="P6L01_001142"
     CDS             complement(13219..14085)
                     /locus_tag="P6L01_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDP3030220.1"
                     /translation="MSHNENLKLAQRGAYLSLIVYIILSIVKYVTGFVFNSAAVRADA
                     LNNMTDIIVSLAVIIGLKISIKPADRNHPYGHLKSENISSLLVSFVIMFVGIQVVIQN
                     APRLFKEDDVVPNAITIIVSLISGLVMLIVFAVNQRLAKRTKSSSLNSAAKDNLSDSL
                     VSIGTAIGLIFTQIGFPIVDIILATLLGLLIVYTGFGIFKEAIFMLSDGFNETELEAY
                     RNDILEVDEVQEVKSIKGRYHGSSVFIDVTIVVDANLSLVEAHQICDNVEHHLHKKGI
                     SSVYVHPEPDHL"
BASE COUNT         4699 a         2608 c         1919 g         5017 t
ORIGIN      
        1 ctactcaaca tttatttgag caagcatcaa tatatcggtc gcttgtagtg tatattatta
       61 tcttaaaatg gtggttggcc taatattgtt tcgtcaaagc gctcgggtat caatactttg
      121 cgcatgatca cacctaaatc gccatcatca ttttcatgtt cgctgtatat ttcataacct
      181 cttttttcat aaattttaag taaccacgga tgcaatcttg cagatgtacc taaagtaact
      241 gccgctgact ttaacgtatc tcgcaaaaat gcttcttcaa cataagtaag taattggcta
      301 ccatagcctt tcccttcata ctcaggattt gtcgcaaacc accagacaaa aggatagccc
      361 gaaatacttt tcacacttcc ccaaggatat ctaaccgtaa tcgtagatat aatttcatca
      421 tcaattgtca tgacaaatgt agtattttta tctatatttt ctttaacagc atctaaatta
      481 gcattaactg aaggccaatc aatacctagt tctcttagag gcgtaaatgc ttcatgcatg
      541 agttgttgca atttttctgc atcttgttca cttgcgagtc gaatcatcgt ttttgtcata
      601 ttaatcccca ctctttttta aatgatttaa ccatatttta tttttaaaat aaatatccat
      661 caaagtgtat caataaattt atcacatgtc agaaagtatg cttcatctga atacaccaat
      721 actctcatga aacttattaa aaattactct ctcaacgtaa aaaaaccatt caaattcatg
      781 aatggtttgg aagaatgatt cattgttacg ctatttaatc actacatctt aattattgtt
      841 gctctaaacg attacgctta ccatttaaga aagcataaac gagacctaca aaaataccgc
      901 caccgacaaa gttacctaag aaagcaaaaa cgatattttt taaaacatgt aaccatgaaa
      961 ctgcatcaag gttaaagaat accatacctg catatagacc tgcattgaac acaacgtgct
     1021 catatcccat gtatacaaag accacgacac cacaagctat gaagaatgcc tttgttaagc
     1081 cgcctttgaa ttgcatagag atgaaaatac caatattaat aaagaagtta cagaaaatac
     1141 cttttgtaaa aatattcaac catgttgaat caacagtctt tttctgaact aaagctgtta
     1201 aagcttgtgt catttcaggt gtcatgacat gcgcaaattt cattaagaaa aataacacaa
     1261 acccacctaa aatatttcct aagaaacaat ataataaaat ccaagtcatc ttcttaacag
     1321 aaacgacttt ataataccag cctactgtaa agtacatgaa gttacttgtt aataattcag
     1381 agttagttaa tacaactaaa atcaaaccta aactaaacgc aatggctccc attaaattga
     1441 taagtccatc tacgtgagta ttcgcaaact gcgtctttat tccgaacata aatacagtaa
     1501 cgattgagag taaaaatccg gccatcattg ctttaagcat atatcggcca ggtgtttgct
     1561 gtaccattac ttgcttcatt tgtactgaag atgatattga atcaacaatt gcgcctgttg
     1621 aatatgtatc ttcaactgtt ttcttatttt ctatcacagt acttccccca tttactttga
     1681 tcaaataaaa tttactttct aattatatta gtaaatcaat gtaattgata gattaaattt
     1741 cagaaaaatc tttttattct ctcaaaaatg tagttttctt tcataaaaac agcgagataa
     1801 atcgcgtgat tggctaatat ctttatgatt taagatatta cgacgctcat tttatctcgc
     1861 tttatgtaaa gtattactta ttctttttta aatttgaaaa gtaatgtaaa ccatatgcaa
     1921 atatactgta accaattgca gtacgtaaaa taaaccttat aactttatta ggtacacatt
     1981 ttgacgttac tgaaatagaa gatagtagtg ttgtgaaaat atataatatt aaaactttga
     2041 tataacgttc taccatcgaa tcatccctca tttttataat attacaagat tattataaac
     2101 cgaatgaaac aaaaaagttt ttacatattt tcaacaactt tatttcaata aagcatcaag
     2161 ttgatctaaa gtttgattca taccttgttc aacacccatg tttatgactt gttgagcggc
     2221 ttcttttgtt ggaaataccg atgttgatgt cactgtcgtt ttcgttgtat tgctcttgga
     2281 aaatgacaaa gtaattttca tgcttggcat ttttgtatct tttgcacctt gaggtgtcgc
     2341 aaatgagtca atatattcaa ttaaatatgg acgcttaacc gttttatatt ctgctaatgt
     2401 atagctggtc attgtaggtg ttttaatcgc ataaaatgca tcaccgcctg aaacagcatt
     2461 aaagcgaaat acttttgtgc tagccccttt tggatgaaac catttttcaa ataattcttt
     2521 tgtcgtatag gcatcaaaca ccttttcaat cggtgcttct atcgttcttg aaaaaataat
     2581 tttattatct tcaactttaa tcgtcatctt cccactccct atcttttata tttactcatc
     2641 ttaacatgca ttgagtaaca tatgttacca aaccatttgt tataaactat agtaatatta
     2701 atcattccct ctgttgaaat tgataactac gaaataaaaa acacactcta ttcagctatt
     2761 aacagttgag tagagtgtgc aaacctgttg aattaatgtg ctaacatttc ttctttgatt
     2821 tggtctttat ttaattttga aggataatat gttggccagt tatccatttc ttttaataac
     2881 gcgtcattat cattacccat aaagatatgg aagtgttctg cctttttagg tgcaatgttg
     2941 tgatcgctaa attggatgaa tttcggtaag tctttatcat taccatcgac caatttaaac
     3001 ataaatctca cgccacggtt acctttagga tatttcaatg ttttcttacc attgtattca
     3061 tatttaccag tgtgtttctt accatcttta gtaaatgtaa tttcatttcc ttttatatca
     3121 atgttagtaa tatcagtttt atatcctttg tcataataag cttttaaatc tttagcagat
     3181 ttcttcggat cattttcagc tttatgttcc atcacttcat caagcgtacc gtcttttaag
     3241 taagggtaaa cagattgcca ttcaccagca taatcgctta attcacggtc tttaacttgc
     3301 tcatctttaa aatacccatc agaaattgct ttatcgtgtt tactttcagc tttgtcgtct
     3361 ttcactttaa caccactgtc taaagcttta ccgatatttt caatattcga tttcattaat
     3421 gattgatacg taatattatc ttttttctgt tgttctttat ttaaagactc catgttgtag
     3481 aattttaaag gcttcgcatc tgtttcttta cgaattgttt cagtcacttt attcgcaaca
     3541 ttatcttcgt aaagaatata tttagcattg ctatctctaa tttctttaac aattttagtt
     3601 aattcttttt gtgatggatc ttcagcattc atattttgaa tacctttttg aacaaaacca
     3661 taacgatcag ctaagtatcc aattgattca tgtgaaataa atactgcatt accttgctta
     3721 tcttttgtaa cttgcttcat atcgttatca attttcttaa gatcgtcgtt taattttttg
     3781 tagtttttct catagtcatc tttatgtttt ggatcttttt tcactaattc atctttaatt
     3841 tctttagcga aagtttggtt aattttagga tctaaccata cgtgtggatc atatccacca
     3901 tgatgatgat gttcttcttt ctcatgatca tgcccctcat gttcatgctc ttcaccatgc
     3961 tcgtgttgat cagttaaaag ctttgcttta tctaatttat cctctaaaga ccgtttttta
     4021 tctttatctt tgatagtaga tgcaactttc ttagcaaccg gatctaaatt atcccctgta
     4081 tacataaaca agtctgactt gcttgcactt aatatatctt tttgtgttgg ttcatagcta
     4141 tgtaaatctg tccctgctgg atagattgat gataccttca cgtgttttcc accaatttgc
     4201 tctgcaaatg attgcaatgg ataaactgtc gttttaattg ttaccttgcc atctttatct
     4261 tttccatcat cattcccaca tgcggctaat gataaagtta cggctggtac aagaagtaac
     4321 atacctaatt tctttttcat agtcaccctc ctaaatagta atctttacgt tttaaaatta
     4381 tatactcatg ctacttaaaa tacaagcact gtttttataa ataatataaa gtcgaatcat
     4441 actttaggta tttaccactt ttcgttttat ctaatacatg actattgtac aaatatgata
     4501 cagaaaactt tatagtattt acgaacgtca taatataaga aaaaaagaca atctctatca
     4561 tattgctaat agaaattgcc ttttcatatt gtcctaaaat aacttaccct atttttatag
     4621 tgtgactttt atatttaata atggtattta catgatgcta tttttatatg attgtcatct
     4681 actgtataaa ccaatctatg ttcgtgattg atacgtctac tataataccc agtcagattc
     4741 gattttaact tttcaggctt acctatacct tccaatgcac catttctgtc aatactttta
     4801 agtaactcat taatcttctt caccattttt ttattgttct gctgaaaata cttataatct
     4861 tcaaaggctt gaggcgaaaa cgtaatattt aacctagcca ttatacatct atatctttcg
     4921 ttatagtttt cccacgttct agatctgcaa ttgattgagc taagtgttca gcattatttg
     4981 ggttctgttg gaggtaaagt gtttccatca tggagttata atctgattct gacatgatta
     5041 ctacgttttt atcatcagta gatgttacag ttaccatatc actatcatca tttacttttg
     5101 tcataagtgc ctttaaattc tgtcgagcgt atgaataatt tttaataatc atatttatcg
     5161 cctcctttta acacacctca attgtacaaa tatctgtaca ataaaacaag cgacacagaa
     5221 aaaaactact aatcaagatt tcgcacgggg aagaatcaat acctatcctg tactgattcc
     5281 ctactaaaca atgtttacat gataatttct acctatttag acttcataca ttaaaaagac
     5341 aatctctatc atatcgctaa tagaaattgc cttttcatat tgtcctaata aattgatatc
     5401 acttacttat ctcaacaatt aagactattt tattttatct tttaataact tctcataact
     5461 ttcataatca tatggatctt caactttctc gccattaata aatgcagttg gtgtcgtttt
     5521 tatatgatta tctttcgcta tttttttatc tttctctgca gctttccaag acttgctatc
     5581 ttttgtcttg taatctttta taattttatt ttccgtctct ttatctaaat gcagttgttt
     5641 aatatgttta tctaatagtt cttttgttaa ccattcttta ttttcatctt gctgggcagc
     5701 aaataattgc ttttgaaaat ctaaaaatga tttaggtgca tacatcaata ctgcatgact
     5761 cgcacgcgaa ccaacaattg agtctttacc taagaaagct aaattgacaa attggtattc
     5821 cactttgtga ttatctatat aatttttacg caactttggc atgacttttt catctaattc
     5881 tttacaataa ggacatttat agtcgccata tatgacaact aatggtttgc cgtttttcga
     5941 ggatgtcgtt gctgattctt ttttaccgca agctgttaaa attaacatgc tcactataaa
     6001 taatgttagt aattttttag tcataataac tcctgttcat atctagttcg tcatattttc
     6061 ttcatgataa tccgcatcat gttgttgata atatcgtctc gaatcaaaat cttgcttata
     6121 tgatacacgt ttatcactaa aatcatatgc ataataatgc acttcatcat cattactcaa
     6181 aggtttataa gctaaaatga taattttatc cttatcatag acataaatat tagcatcttt
     6241 gcgttcaaaa tgatcaacac gatcaccgtt gccttttttg gcaagttgtt cttgtttttg
     6301 attttgaatt ttaactgctt catctatttc tttcacatat ttatcattac cacatcctga
     6361 tataaagact agtaataata ataccgtcgc cacaaatcgc ttcaatgctc ccactcctac
     6421 tatcaacttc caactaactt tcaatataat taatactata caccgtcaaa cattaatgcg
     6481 ctacaactac taccaatgca tttaatataa atatccaaag aaataacagt tatgtatagt
     6541 caaagaaaaa tatatttcat tttctatgtg tatttgtaat cagtttttga taaaataaaa
     6601 aaatagcatt attaataaat tcaaaaatta cagttcaatt cagagaggga tgggataaaa
     6661 tgaactttgt aacgttgact tcggatgagt tcaatgcgtt tacaacaaag catttttcac
     6721 attacacaca atcagctatt cattacaatc atagagttga tttaaaaggc gatgtgcatc
     6781 ttgtaggggt taaagatgac aatggtcaag tgattgcagg atgcttattg acagaagcac
     6841 gcacacttaa atttttcaaa tatttttata cacatcgcgg gccagtgatg gattatacaa
     6901 atcaatcatt agtagcattt ttctttaaag cattaacgtc atatttaaag aaacacaatt
     6961 gtttatatgt ccttgtagat ccatatttaa ttgaaaattt acgcaatgca gacggtgaaa
     7021 ttgttaaatc ttatgataac cgagcatttg ttagaacaat ggataaatta ggttataaac
     7081 accaaggttt ccctgtaggt tatgattcaa tgagccaaat ccgttggctg tcagtgttag
     7141 atttaaaaga taagactgaa gaccaacttt taaaagaaat ggattatcaa acgagacgta
     7201 atattaaaaa aacatatgat attggtgtca aaactaaaac gttaacgatt gatgaaacgc
     7261 aaactttttt cgacttattc catatggctg aggaaaagca cggtttcaaa ttccgtgagt
     7321 taccatactt tgaagaaatg caaaagttat acgatgacca cgccatgtta aagttggcgt
     7381 atattgattt aaacgagtat ttaaaaacgt tacaattaaa gcaacaacaa ttaacagctg
     7441 aactctcagg tgttgaagaa gcattggaag aaagtcctaa ttcaaagaaa aataaaacga
     7501 aacgcacaca gctagaacaa cagctaaata gcaataagcg taaaatcgac aatacaatag
     7561 aacaaattga acaagatggt gcagtcttaa acttagcttc tgctttattt atctacaatg
     7621 agcatgaagt ttattactta tctagtggtt caaaccctaa atataatgct tatatgggtg
     7681 cctaccattt gcagtgggaa atgattaagt ttgctaaagc gcatcatatt gatcgctata
     7741 atttttatgg tattaccggt gactattcag agtcatcaga agactatggc gtccaacaat
     7801 tcaaaaaagg ttttaacgca catgtagaag agtatatcgg tgactttatt aagcctatca
     7861 aaccattact atataaggta caaacgtact taaatcataa acgtcgttaa aatatcgctc
     7921 caagattatg acaacataac aaagaaccac gcatcatgta tatggcatca tcaccatact
     7981 aaaacatgat acgtggtttt atttgattat tcttcattta taacatttaa gaaacgtctt
     8041 aattcttctg ttttcggacg attgaacatc tcttctggag ccccttgttc tccgatcatg
     8101 ccttcatgaa taaatacaat gttattagat acttcttttg caaaacgcat ttcatgtgtc
     8161 acaatgacca ttgtcatgcc ttcattagcc aaatctttaa taacctttaa aacatcattc
     8221 acaagttcag gatcaagtgc agatgttggt tcatcaaaca acatcacttt agggttcatt
     8281 gctagtgctc ttgcaatagc aacacgttgt tgttgaccac ctgataatgc atgtggacgt
     8341 tgatctttga catgtgttaa accaactttc tcaagtaact caagtgattt cccacgtgcc
     8401 tcatcctttt tcaacttttt aactgtgata agaccttcca ttacattttc taatgccgtc
     8461 ttatgcggaa aaaggttata actttgaaat accatgccag actgtttacg aacttctatt
     8521 tgtgattttt tatctttaga tgtatatgtt ttgccgttaa cataaactgt accttcagtt
     8581 ggaatttcta atgcattaat catacgtaac aatgttgttt tacctgaacc agatcgaccg
     8641 attaaggtta caacctcacc ttgttcaaca gataaatcaa tacctttgat gacttcaaca
     8701 tcattaaatg atttatggat attgttcaat tgaatcatga gcgataccct ctttcaatat
     8761 aagattcata gataccttgg ataattgaaa taataaagca taccacccag tacattagcg
     8821 caactaacac ataaattgtt aaatattcat atgttgtaga cgcaacttct tgagcttttc
     8881 taaacatttc agccactaaa ataaatccta ataatgatgt atctttgatt aaacttaaaa
     8941 atgtattacc tagtgcagga atcgacactc gaattgcctg cggtaaaatg atacgttgta
     9001 tcgtttgacc atacgtcata ccgatggaat atgcagcttc tgtttgtcct ttcggtatag
     9061 aaataatacc gccacgaata atttccgaag catacgcacc tacatttaat gataaaccaa
     9121 taatagcagc cactacaggt gccaatgtcc attggttgtc agcgtcattt gttaataatc
     9181 tacctaattc tggaatacca taaaatatga taaatagttg tactatcatt ggtgttcctc
     9241 gaataataga tacatagaca cgtgaaatac ttcttaaaat tttactcgtt gaaattcgca
     9301 ttaatgctgt aaataatgca ataatcaatc ctaaaacaaa tgtaactaat gtaataggaa
     9361 ttgaatattt gaccaaacct tctagcatag gtccgaaagc ttgttttgca gcatctagcg
     9421 catgttgttg ttcgctattt agatttagaa acatcttgac caaaccattt cttacctatt
     9481 ttagctaatt caccgttttc ctcgattttt ttcaagccat cattgaattt ttgaactgtt
     9541 tcatcatctg cttttttaga aaatgcaaat gcagatctac tttgttcagc attacctttg
     9601 attgctttga tcttagcatt aggtttttgt tttttataat ccaagtatga cagactatca
     9661 ttaaatgtac catcaacacg cttagacaat aataaatcca ttgattggtt aaagccatca
     9721 acttttgtaa tatcagcacc tttatcctta gctaatttac cataattaga tgtgaatgtt
     9781 tgtgctaact tcttaccttt aacatcatca aaatctttaa tatctttttc attttcacga
     9841 ataactaaaa cagcacttga gaatgtgtaa ggcttagaaa atttatattt cttttctcta
     9901 tcaggattaa tacctacttg gttcgcgatt acatcaaaac gccctgcgtc taaacctgca
     9961 aacatagaat cccaagaagt ttcattaaat ttaagtttta aaccttcttc tttagccact
    10021 gctttaataa catcaatatc gtaaccagtt aatttaccat ctttatcatg gaaactaaat
    10081 ggtgcataag tcccctcagt accaacatta attgtcttgc tatctttatt agcttccttg
    10141 tctttcgacg aattgtttcc acatgcagcc aatatgaaaa cgaaagctat catcacaaat
    10201 aaaagtcttt tcatagaata tatctcctta ttcttattat tctaatcgga ataaaaatat
    10261 tttaatctga aatgccggat aagtcaatat gttgaacttt tgctaaaaag ctttatatag
    10321 ctagcaactt atatttattg acgagaatca acttcttttt tcttcccttg taacataaat
    10381 gaacaaaaga aactaacaac tgtaatcaat aatgcaaata aaaatccagc gtgataacca
    10441 tgcaacatcg tatcaacttt aatttgattc atcatatcaa ttttgttcat gccattatag
    10501 tctgacatag ttggagaaaa gtttttgctt gcatgagata aaatagtaat taatgcagct
    10561 gttccgatag aaccagcaat ttgttgtact gtgtttgtca tagatgaacc gtgtgcattc
    10621 atttctggct ttaattgatt catcgtatgg gtcattactg gaattaatcc taacgcgata
    10681 ccaaccattc gaatcgcata aactgttgcc aacatgattg ttgatgtttg ttcatccatt
    10741 acaacaaaat aagccgtagt aataacaaca atcgccatac ctactaaagc aagctttcta
    10801 ggaccaaact tttcatataa agcacccgaa gtcatcgaca taaatgccat aacaattgct
    10861 cctggtaata aagttaatcc agaatctaaa gctgaacgat gcaataaatt ttgcacaaag
    10921 ataggtaaaa tcgtttccga accaatcatc gataccattg tgacacccat aataatgata
    10981 ccaactgtaa attgtttatt tttaaataca gcaaaattta ataatggtgt tgatagccga
    11041 tattgacgta atacaaataa tgcgactaaa acaacgcctc caatcataga tactaatacg
    11101 ataggattat caaaaccttt ttctgaaatt gaactcgttc catacaataa cccgccgaaa
    11161 cctagtacag aaaaaatgac agaaataaaa tcaataggta ctttaattgt agttccaaca
    11221 ttttttatcg ttttaaatcc aattaaaaat gtaacagctg caattggagc gacaacatgg
    11281 aaaagcgatc tccagtttaa atattcaaca aggtaacctg aaagagtagg tccaatagct
    11341 ggtgctaaac caattaccaa accaaatatc cccatatatt taccgcgttc atgcggttgg
    11401 aaaacatcca aaatcgttgt catcatcaat ggcatcataa tacctgcgcc taacgcctga
    11461 ataattctag caactaataa tgttgcaaag tgcggactca agccgccgag taatgtacct
    11521 aagaaaaaga tagaaatacc cactaaaaac acttgtcttg ttgtataacg ttgtataaca
    11581 agcgcggaca aaggaataac aatcccatta gtcaataaaa aagctgttgt cagccattga
    11641 acttgtgtat atgaaatttc aaaatctttc ataatacttg gtaaagccgt cactaatagt
    11701 gtttcattta ataatccaaa aaatccaccc atcagcatag taataatcat cgttaatttt
    11761 tgtttcttcg acatttaaag cttccccctc accatctaaa aaacgacaca catttttaga
    11821 tatccggtaa ttatacccat tttccaaaaa attagcacga ctttattaca atttctttaa
    11881 aacgtaatca ttactattta catatgagta gttatcggtc ataatatatt tacaaggagg
    11941 gatagagatg aaattagatt tacagactgc tcgtcgaaat ttaaatagcc ctaacattaa
    12001 aacaagaaag cgtgctttaa agattattaa gcaacacaaa agagcgaaat aattgtttta
    12061 atttgactat attgtatgtc taactatatt ttcgagtatc cctagctcgt taatataaac
    12121 tctatactta actatttcct tacattactt cacatattta agagctgcat atacactcac
    12181 tccttgtgta catgcagctc tttttttata agtagtattt atctataact tctaaatcat
    12241 ccgttaattc ataaacaagc ggtgcacctg ttttaatttc ataattaatg atatcttcat
    12301 ctgacacatc ttcaagatat ttaattaatg cgcgaattga atttccgtgt gcagaaacta
    12361 ataccgtttg accatctagc aaatattgtg aaatatgatc tgtccaaaat ggtatcactc
    12421 gaactaaagt atctttcaga ctttcagaat aaggcatcat acgtttatct aaatgattat
    12481 atcgacgatc agctaagtaa gcttcacgtt gttcttcggt ttcagcaggt ggtttcacat
    12541 cataagaacg acgccaaata tgtacttgtt cttctccaaa ttcttttcta gcatcatctt
    12601 tatttaagcc ttgcaatcca ccatagtggc gttcatttaa acgccagctt ttatatacag
    12661 gaatccattg ttgtttagat tcagttaaaa tataatgcgt tgtatctaaa gcacgtgtta
    12721 ataacgatgt aaaagctaca tcgatggcaa tgttattttc tcttactttt tcacctgctc
    12781 tagtcgcttc attaatacct tgttcagata aattaacatc ttcccatcca gtaaataagt
    12841 ttttagcatt ccactcgctt tgtccatgac gacataaaat taattttggc ataacttata
    12901 acctctttcc tttaatcttt aggacgttat ttcagggata ttttaagatg atttaatctt
    12961 agtaattttc ttttcaatat attttaaaat tcaatgatta aatcaattat cactagacat
    13021 taaatacata aatcctattc cccattttca ttttttaatt cataaatgaa tcaataacca
    13081 cctaataaca aatcatatta tacacctttg ttcgctattt ttctaaggtt taaaaaatat
    13141 ttttaggtaa gcctaaaaat agatgtaata aaaacgcctc ctcagatatt tatatatcta
    13201 tgaagacgtt taaatacatt atagatggtc tggttctggg tgaacgtata ctgaggaaat
    13261 accttttttg tgcaaatgat gttcgacatt gtcacaaatt tgatgcgctt ctactaagga
    13321 aaggttagca tctacaacaa ttgtgacatc aataaacaca ctacttccat ggtaacgccc
    13381 tttaatactt ttaacttctt gtacttcatc aacttctaaa atatcattgc gatacgcttc
    13441 taattcagtt tcattgaaac catcactcaa cataaaaatt gcttctttaa aaataccaaa
    13501 accagtataa acgattagta agcctagtaa tgttgctaaa ataatatcga caattgggaa
    13561 accgatttgc gtaaaaatta atcctatcgc tgttccaatg ctgactaaac tatctgataa
    13621 attatctttg gcagccgaat ttaaagaact acttttcgtt cttttcgcta gtctttgatt
    13681 gactgcaaat acaatcaaca ttacaagacc actgattaag ctgacgataa ttgttattgc
    13741 gttaggtaca acgtcatctt ctttgaacaa acgaggtgca ttttgaataa ctacttggat
    13801 acctacaaac ataatgacaa atgacaccaa taatgaagaa atattttcag acttcaaatg
    13861 gccataagga tgatttcgat cggcaggttt aattgaaatt ttcaatccaa taataacagc
    13921 taaagaaacg ataatatctg tcatattgtt taatgcgtcg gctcttacag ctgcagagtt
    13981 aaagacaaaa cccgtgacat acttaacaat agataagatt atatatacaa ttaaactcaa
    14041 ataagcaccg cgttgcgcca atttaagatt ttcattatga gacatgcgtt gaaccacctt
    14101 gaattagtat agtaacaata ttatgaatga ttcattttaa ttttacaacg tttttaattt
    14161 ttataaattt ttataaaatt aaactaattt attcattcgc aacccctaaa aataattttt
    14221 agcctttctt caaatttatt tgt
//
LOCUS       DAOBBH010000051        14179 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.051, whole genome shotgun sequence.
ACCESSION   DAOBBH010000051 DAOBBH010000000
VERSION     DAOBBH010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14179
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.051"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(133..1326)
                     /locus_tag="P6L01_001143"
     CDS             complement(133..1326)
                     /locus_tag="P6L01_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDP3030221.1"
                     /translation="MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVI
                     GIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRIILIINAIALIIYGFTGLEGYFVARV
                     MQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNIS
                     LFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGI
                     IMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVAARFYLRKYIPSDGMWHP
                     KYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVG
                     RNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSFLKKPTPR
                     PASSL"
     gene            complement(1338..2087)
                     /locus_tag="P6L01_001144"
     CDS             complement(1338..2087)
                     /locus_tag="P6L01_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3030222.1"
                     /translation="MIKVTDVEKSYQSAHVFKRRRTPIVKGVSFECPIGATIAIIGES
                     GSGKSTLSRMILGIEKPDKGCVTLNDLPMHKKKVRRHQIGAVFQDYTSSLHPFQTVRE
                     ILFEVMCQCDGQPKEVMEVQAITLLEEVGLSKAYMDKYPNMLSGGEAQRVAIARAICI
                     NPKYILFDEAISSLDMSIQTQILDLLIHLRETRQLSYIFITHDIQAATYLCDQLIIFK
                     NGKIEEQIPTSALHKSDNAYTRELIEKQLSF"
     gene            complement(2080..2895)
                     /locus_tag="P6L01_001145"
     CDS             complement(2080..2895)
                     /locus_tag="P6L01_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3030223.1"
                     /translation="MTLLTVKHLTITDTWTDQPLVSDVNFTLTKGETLGVIGESGSGK
                     SITCKSIIGLNPERLGVTGEIIFDGTSMLSLSESQRKKYRGKDIAMVMQQGSRAFDPS
                     TTVGKQMFETMKVHTSMSTQEIEKTLIEYMDYLSLKDPKRILKSYPYMLSGGMLQRLM
                     IALALALKPKLIIADEPTTALDTITQYDVLEAFIDIKKHFDCAMIFISHDLTVINKIA
                     DRVVVMKNGQLIEHGTRESVLHHPEHVYTKYLLSTKKKINDHFKHVMRGDVHD"
     gene            complement(2892..3761)
                     /locus_tag="P6L01_001146"
     CDS             complement(2892..3761)
                     /locus_tag="P6L01_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3030224.1"
                     /translation="MIILKRLLQDKGAVIALGIIVLYVFLGLAAPLVTFYDPNHIDTA
                     NKFAGISFQHLLGTDHLGRDILTRLIYAIRPSLLYVFVALFVSVLIGSILGFLSGYFQ
                     GFVDALIMRACDVMLAFPSYVVTLALIALFGMGAENIIMAFILTRWAWFCRVIRTSVM
                     QYTASDHVRFAKTIGMNDMKIIHKHIMPLTLADIAIISSSSMCSMILQISGFSFLGLG
                     VKAPTAEWGMMLNEARKVMFTHPEMMFAPGIAIVIIVMAFNFLSDALQIAIDPRISSK
                     DKLRSVKKGVVQS"
     gene            complement(3758..4693)
                     /locus_tag="P6L01_001147"
     CDS             complement(3758..4693)
                     /locus_tag="P6L01_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3030225.1"
                     /translation="MFKFILKRIALMFPLVIVVSFMTFLLTYITNENPAVTILHAQGT
                     PNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNT
                     LKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVS
                     VKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVK
                     SITLMLHVLRNAIQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPV
                     IQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR"
     gene            complement(4706..6304)
                     /gene="cntA"
                     /locus_tag="P6L01_001148"
     CDS             complement(4706..6304)
                     /gene="cntA"
                     /locus_tag="P6L01_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine-dependent metal ABC transporter
                     substrate-binding protein CntA"
                     /protein_id="HDP3030226.1"
                     /translation="MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDI
                     GDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFH
                     DGTTFDADAVKKNIDAVQQNKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
                     LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSK
                     LNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQP
                     MNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDI
                     NFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEY
                     LQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGY
                     ESATSGIENKDKIYNSIDDAFKIQNGKERSDAYKNILKQIDDEGIFIPISHGSMTVVA
                     PKDLEKVSFTQSQYELPFNEMQYK"
     gene            complement(6447..7748)
                     /gene="cntM"
                     /locus_tag="P6L01_001149"
     CDS             complement(6447..7748)
                     /gene="cntM"
                     /locus_tag="P6L01_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_096659686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine dehydrogenase CntM"
                     /protein_id="HDP3030227.1"
                     /translation="MSKLLMIGTGPVAIQLANICYLKSDYEIDMVGRASTSEKSKRLY
                     QAYKKEKQFEVKIQNEAHQHLEGKFEINRLYKDVKNVKGEYETVVMACTADAYYDTLQ
                     QLSLETLQSVKHVILISPTFGSQMIVEQFMSKFSQDIEVISFSTYLGDTRIVDKEAPN
                     HVLTTGVKKKLYMGSTHSNSTMCQRISALAEQLKIQLEVVESPLHAETRNSSLYVHPP
                     LFMNDFSLKAIFEGTDVPVYVYKLFPEGPITMTLIREMRLMWKEMMAILQAFRVPSVN
                     LLQFMVKENYPVRPETLDEGDIEHFEILPDILQEYLLYVRYTAILIDPFSQPDENGHY
                     FDFSAVPFKQVYKNEQDVVQIPRMPSEDYYRTAMIQHIGKMLGIKTPMIDQFLTRYEA
                     SCQAYKDMHQDQQLSSQFNTNLFEGDKALVTKFLEINRTLS"
     gene            complement(7741..8559)
                     /gene="cntL"
                     /locus_tag="P6L01_001150"
     CDS             complement(7741..8559)
                     /gene="cntL"
                     /locus_tag="P6L01_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-histidine (S)-2-aminobutanoyltransferase CntL"
                     /protein_id="HDP3030228.1"
                     /translation="MNNFNNEIKLILQQYLEKFEAHYERVLQDDQYIEALETLMDDYS
                     EFILNPIYEQQFNAWRDVEEKAQLIKSLQYITAQCVKQVEVIRARRLLDGQASTTGYF
                     DNIEHCIDEEFGQCSITSNDKLLLVGSGAYPMTLIQVAKETGASVIGIDIDPQAVDLG
                     RRIVNVLAPNEDITITDQKVSELKDIKDVTHIIFSSTIPLKYSILEELYDLTNENVVV
                     AMRFGDGIKAIFNYPSQETAEDKWQCVNKHMRPQQIFDIALYKKAAIKVGITDV"
     gene            complement(8570..9391)
                     /gene="cntK"
                     /locus_tag="P6L01_001151"
     CDS             complement(8570..9391)
                     /gene="cntK"
                     /locus_tag="P6L01_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine racemase CntK"
                     /protein_id="HDP3030229.1"
                     /translation="MNRQVIEFSKYNPSGNMTILVHSKHDASEYASIANQLMAATHVC
                     CEQVGFIESTQNDDGNDFHLVMSGNEFCGNATMSYIHHLQESHLLKDQQFKVKVSGCS
                     DLVQCAIHDCQYYEVQMPQAHRVVPTTINMGNHSWKALEIIYETYVHYVIPVKQVTTE
                     IQHLVEAFVREQQWSHKYKTVGMMLFDEQRQFLQPLIYIPEIQSLIWENSCGSGTASI
                     GVFNNYQRNDACKDFTVHQPGGSILVTSKRCHQLGYQTSIKGQVTTVATGKAYIE"
     gene            complement(10081..10848)
                     /locus_tag="P6L01_001152"
     CDS             complement(10081..10848)
                     /locus_tag="P6L01_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDP3030230.1"
                     /translation="MNIRHIRNATAVIEYGGKRILIDPMLSDKGSFDPFPNSPRQDQK
                     NPLVELPMAIEDIINDIDLVILTHLHIDHFDPKAIEVLPKDIKIYAQNEEDASEVERY
                     GFTNVSVFNDVTHIGEIELIKTDAQHGHGEILKMTGHVHGMILKHSEEPTLYLAADTV
                     WFEGVEKALKTYQPDVVVLNGGANQFFEGGPLVMDEHDVLKVANTLPLAQIVVVHMEA
                     VNHWHLSRKELNEFINSNDLGNRVVVPNDGELLTFEK"
     gene            complement(10948..11766)
                     /locus_tag="P6L01_001153"
     CDS             complement(10948..11766)
                     /locus_tag="P6L01_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDP3030231.1"
                     /translation="MKRLENKVAVVTGASTGIGQASAIALAQEGAYVLAVDIAEAVSE
                     TVDKIKSNGDNAKAYNVDISDEQQVVDFVSDIKEQFGRIDVLFNNAGVDNAAGRIHEY
                     PIDVYDKIMNVDMRGTILMTKMMLPLMMNQGGSIVNTSSFSGQAEDLYRSGYNAAKGA
                     VINFTKSIAIEYGRDGIRSNAIAPGTIETPLVDKLTGTSEDDAGKTFRENQKWMTPLG
                     RLGKPEEVAKLVVFLASDDSSFITGETIRIDGGVMAYTWPGEMLSDDSWKRTLE"
     gene            12001..13539
                     /locus_tag="P6L01_001154"
     CDS             12001..13539
                     /locus_tag="P6L01_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDP3030232.1"
                     /translation="MTSKHQQKGSIVNRFLNSVEKIGNKLPDPSVLFFLMCVGLAIMT
                     WVISLFNVSVKHPGTHQTIYIKNIISHDGFTMIMNDTIKNFSEFPALGLVLAVMIGIG
                     VAEKTGYFDKLMISVVNRAPRFLILPTIILIGILGSTAGDAATIILPPLAAMLFIKIG
                     YHPIAGLTMAYASAVGGFAANIVVGMQDALVYSFTEPATRIVSDSIKTNVAMNWYFIA
                     ASVVVLLPTILLVTTKLIIPRLGKYDDSLMHDDHEEASSHITDKEAHALKWANISFIV
                     TIILLIITAIPEHSFLRNAKTGSLLDDAPLINGVGLIILVVFLVPGLVYGILSKEIKN
                     TKDLGKMFGDAVGSMGTFIVIVFFAAQLLAYLKWSNLGIIAAVKGAKLLEHQNGIVLI
                     LGIIVLSAMVNMLIGSASAKWGILGPIFVPMLILIGFHPAFTQVIYRVGDSITNPITP
                     MMPYLPLLLTYAQKYDKRMKLGALLSSLMPYSIALSIVWTVFVIIWFLLGIPVGPGGP
                     IFVK"
     gene            complement(13652..14074)
                     /locus_tag="P6L01_001155"
     CDS             complement(13652..14074)
                     /locus_tag="P6L01_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDP3030233.1"
                     /translation="MPYNYKKQNGELMSVMSQGEKFIHQSPVNDELSALIKLLISKIN
                     GCHYCVDIHKKELKELGVTQMKIDEVLSFRHLDLFTDQEKVTLEFAEMLNSIKDFKKF
                     EIIDRLKSFYDEEQIIDLVFVVNQINGWNRLNIISDRL"
BASE COUNT         4372 a         2821 c         2155 g         4831 t
ORIGIN      
        1 ccttgccggg gtgggacgac gaaataaatt tttcgaaaat atcatttctg tcccactccc
       61 atgaatcgcc atgctcaata taccttttaa aatttaaata ttcattaact taatatgctt
      121 taattcactt cattaaagac tactcgctgg acgtggtgtt ggctttttca agaaactcat
      181 aatcattatt acaatgacta acataccaca aattagatac atccatttaa atcctactaa
      241 atcggaaata ggtcccatca atgcgccacc taacgatata cctaagtctg cacaggcaat
      301 aaataaacct aacaacatat tacgacctac ttttggtaag acgaagctta agtatgatgt
      361 taatgttggg tacaccattg cttgcgtcat tcctattaat atcgcactac catagaaaat
      421 aattgcacct acttgcggac caaatgccac tacaaatgac gcgattacta ataatgatag
      481 tacagatacc atatatttag gatgccacat accatctgac ggaatgtatt tccttaagta
      541 aaatcttgcc gcaacaactg cgatggcctg tattgtcaaa aagattcccg cattcgcgaa
      601 tcctaaactc actgtgtata acggtacaaa tgtactaact gcaccaaata caatcgatgc
      661 aacaatcatg ataataccac tgttcaacaa ctctttattt ttgaaaaatt gcgcaaaaac
      721 agttacagcg ttaaacggca ttttttcaat cttatctgac gtatcgggtt cctgttcagc
      781 aaaggtcacg cgatagccaa agaatgttgt tgttaatgcg ataaagataa tgacaattgc
      841 aaatagtgaa atattatttg cattccaaat acctacggca actaatggtc caattaagtt
      901 tggaatcgtt gaaaatagcg agtacaatga tacaccttca gaacgatgtt cctctggtaa
      961 tgcatcaata atacctaact gtaaagacat agaaaagaat gccgtacaca caccttgcat
     1021 aacacgtgct acgaaataac cttctaatcc agtaaaacca taaataatta aagctatggc
     1081 attgatgatc aaaataattc ttaaaacttt aatgggaccg acacgagcaa taatttgtcc
     1141 tgcccatggt cgaaacacca ttgctgttaa catgtatgcc cccataacga taccgataac
     1201 cgtattcgtt gcgcctaaat catgccctcg taacggtata aatacgttta agattgcatt
     1261 ggcactaaag aacatcaatg ttaatatata taatctcaaa aagggccaag ccattgcacc
     1321 tttcattatc tcactcctta gaatgatagt tgtttttcta ttaattctct tgtataagca
     1381 ttgtcacttt tatgcaatgc gcttgtcgga atttgttctt ctatttttcc gtttttaaaa
     1441 ataattaatt gatcacataa atacgtggca gcttgaatat catgtgtgat aaaaatataa
     1501 ctcaactgac gcgtttcacg taaatgaatc aataaatcta atatttgtgt ttgaattgac
     1561 atgtcgagtg aactaatggc ttcatcaaac aaaatatatt tagggttaat acatattgca
     1621 cgcgcaatcg caacacgctg cgcttctcca cctgataaca tattaggata tttatccatg
     1681 tatgccttag atagaccgac ttcttccaac aatgtaattg cttggacttc cataacttct
     1741 ttaggttgtc catcacattg acacatcact tcaaataaga tttctctaac agtctgaaat
     1801 gggtgtaatg atgacgtata atcttgaaat acagcaccaa tttgatgacg tctgactttc
     1861 ttcttatgca tcggtagatc atttaaggtt acacagcctt tatccggttt ctcaatacct
     1921 aatatcatac gactcaacgt cgatttaccg ctaccacttt ctccgataat cgcaatcgtc
     1981 gcaccgattg gacactcaaa tgacacacct ttcacgatag gtgttcgacg acgcttaaaa
     2041 acatgtgcgc tttgatatga tttttcaaca tctgtaactt taatcatgta catcacccct
     2101 catcacatgt ttaaaatgat cattaatctt cttcttcgtt gataataaat acttcgtata
     2161 aacatgttct ggatgatgca agactgattc acgtgtacca tgctctatca gttgaccatt
     2221 tttcatcaca acaacacggt ctgcaatctt gttaataacc gttaaatcat gtgaaatgaa
     2281 aatcatcgca cagtcaaagt gttttttaat atctataaat gcttccagta catcatattg
     2341 tgtaattgta tctaaagccg ttgtcggctc atcagcaatg attaactttg gtttcaaagc
     2401 taacgctaaa gcaatcatca atcgctgtaa cattcctcct gataacatgt aagggtatga
     2461 ttttaatata cgtttaggat ctttcaaact taaataatcc atatattcaa tcaatgtctt
     2521 ttcaatttct tgtgtagaca ttgacgtatg tactttcata gtctcaaaca tttgtttacc
     2581 gacagtagtt gatgggtcaa aggcacgact accttgttgc atgaccatcg caatatcttt
     2641 accacggtac tttttccgtt gcgattcaga taatgacaac attgatgtac catcaaagat
     2701 aatttcacct gtcaccccga gtcgttcggg attcaaacca ataatcgatt tgcatgtaat
     2761 tgatttaccg ctaccacttt ctccaataac gcctaaagtt tcacccttag ttaatgtaaa
     2821 attcacatca ctcacgagtg gttgatctgt ccaggtatct gtaatcgtca aatgcttaac
     2881 tgttaacaat gtcatgattg caccactcct tttttcacag aacgaagttt atctttagaa
     2941 gagatgcggg gatcaatagc aatttgtaaa gcatcggata agaagttaaa tgccatcact
     3001 ataatcacta tggcaatacc tggcgcaaac atcatttcag gatgtgtaaa catcactttt
     3061 ctagcttcgt taagcatcat gccccactct gcagtaggcg ctttgacacc taatcctaaa
     3121 aatgaaaagc cagatatttg caagatcatt gaacacatcg aactactaga gatgatagca
     3181 atatctgcta atgttaacgg cataatatgt ttgtgaataa ttttcatatc attcataccg
     3241 attgttttag caaatctgac atggtcagaa gcagtgtact gcataacact tgtacgtata
     3301 acacgacaga accatgccca acgcgtcaaa ataaatgcca tgataatatt ttcggcaccc
     3361 attccaaaca atgcaattaa tgctaacgtt acaacataac ttgggaatgc caacataaca
     3421 tcacacgcac gcatgattaa ggcgtcaaca aacccttgga aatatcctga taagaatcct
     3481 aaaatagatc caataagtac agaaacaaat agcgcaacaa agacatataa caaacttggt
     3541 ctaatcgcat aaattaacct agttaaaata tctctaccta aatggtcagt acctagtaga
     3601 tgttgaaaac tgatgccagc aaatttgttt gctgtatcga tatggttagg atcataaaat
     3661 gtcacaagtg gtgctgctaa tcctaaaaag acatataata caataatgcc taaagcaatt
     3721 actgcacctt tatcttgtaa taatcgtttt aaaattatca tcgtgcgccc tcccttaatc
     3781 ttggatttaa tagcgcatta atgatatctg ctaatgtatt aaatacaata aataataccg
     3841 ctacaattaa tacatatgct tgaatgactg gaaaatcgtg ttcaagtatt gcttttaaac
     3901 ttaattgacc tagtccaggc catgcaaaga tatactcgat aacaactagt ccacccatta
     3961 tcattggtat agacatacaa aagattgata ccgcaacttg tatagcatta cgcaacacat
     4021 gcaacattaa tgtgatagat ttcacaccgc ttgctcttaa ataaagtaca taatcttcat
     4081 ttaattgttc caccatcgag cgtctaacat ttctaaagta aataccagca taggcaatcg
     4141 taataacgat cactggcaat atgtaacttt ctggacctgt taatccagaa gtcggcaata
     4201 tgtttaactt cactgaaacg taaataataa gtattgaagc tatccaatat gatggtaatg
     4261 cagttagaaa gaaagccact gaacgtatcg cacgatcagt gaactttcct ctttttaatg
     4321 cactaactac acctaaaata attgatgtaa tcatcaccat aacacttgaa attattgtta
     4381 atttcaatgt gttcataaat gctgggccaa tgcgttcagc aacaggatcc cctgtaatgt
     4441 aacttgtacc aaaattaaat tgcatcgctt caagtaacca gtttttatat tgaattaata
     4501 atggatcatt gaaaccgtac ttttcattcg tttctgcaat caactctggt gttacatttg
     4561 gcgttccttg tgcatgtaaa attgtcacag ctggattttc atttgtaata tacgtcaata
     4621 gaaatgtcat aaaacttact acaatcacta atggaaacat gagcgcaata cgttttaaga
     4681 taaatttgaa catctaattg ctcctttatt tatactgcat ttcattgaat ggtaactcat
     4741 actgtgattg tgtgaatgat actttttcta aatctttcgg cgcaacaact gtcatactac
     4801 cgtgtgaaat agggataaag ataccttcat catcaatttg tttcaaaatg tttttataag
     4861 cgtctgaacg ctctttaccg ttttggattt taaatgcgtc atcaatgctg ttgtatattt
     4921 tatctttgtt ctcaatgcct gatgttgcac tttcataacc atttttcgct ttaaatgctg
     4981 caatagtact ttgtggatcg tacaataatc cccaagtttg gttgaacatt aagtcataat
     5041 caccagaagt acgacgttca gcaattttat ctgatgtttc gccattgatg tttaacttaa
     5101 tacccatttt cttaaattct gcttgtaagt attctgcttg ttctttttga cttgaagaac
     5161 ctttgtcata gtacattgcc atttcaaggt ttttaccatc tttttgacga acatcactgt
     5221 ctttaccttt cttccaacca gcttcatcta ataatgattc tgctttttta aggtcatact
     5281 tacgtgttgg catatcgaaa ttaatgtctg ttacattttt cgcaaataat tgagttgctg
     5341 gtttttcttg accatctaaa atttctttgg caattttatc tctgtttacc atatgaccaa
     5401 tcgcttgtct gactgttttg tcactgacag cgttatcttt tttaccagaa ttgacaacta
     5461 acattttcgt attcataggt tgactacgct taacttgata gtcacctgta tctttcaatt
     5521 gttttaaaga gtctttgtct aagctatccg tacctctatc atctgtgaag gcaaagttcg
     5581 tttcaccttt tttcattgat aggaatgctg tttcaccagc aggcattact tttgcttgta
     5641 ctttgttaag tttagacttt tcgccccagt attgatcatt tttgttaaag tctgcagact
     5701 catctttttt gtgttcacct aatttaaatg gaccagtacc atcgaacttt ttaacgccat
     5761 cttttgttgt accgttttta aagtcttttg gagacacaaa tacatatgga cgaggcatcg
     5821 ctaattcagc caatgcaggt tgatatgctt ctttcaaatt caattcaacc gtgtacttat
     5881 ctttaacttt aacattgtca attaatgttg aaatctttaa ccaagaatgc aattttttat
     5941 tttgttgaac cgcatcaata tttttcttaa ctgcgtcagc atcaaatgtt gttccatcat
     6001 ggaatttcac atcatctctt aaatggaatg tataagtctt cccatcttca gacacatccc
     6061 attttttagc tagtaaaggc ttaataccat ctttcgtgtt acgtacaagc ggctcgtata
     6121 tcatactttc agcagacatt gatccaccgt aaacatgcgg attcatatca ccgatatctt
     6181 taaccgtcgt atacgttaat tgcttgtttt cttttttctc ctctaaacct ttattaccgc
     6241 cacaaccagt taaaattagc cctgatgcaa gtaacattgc actcatttta gttagttttc
     6301 tcatttgctt ttcctctttc taaattgata agttgcatta cttaatcaaa tcgtaatgat
     6361 tatttttctc aattatttgc ttatttttat gtaaatgtca aacataattc aataattttt
     6421 ttctattata aaacttcaaa cccttattat gaaagcgttc tattgatttc caaaaatttt
     6481 gtgacgagtg ccttatctcc ttcaaataga tttgtattaa attgagaaga tagttgttga
     6541 tcttggtgca tatccttgta cgcctggcaa cttgcttcat agcgagttag gaactgatca
     6601 atcattggcg ttttgatacc taacattttc ccaatatgct gaatcatcgc cgttctgtaa
     6661 taatcttcac ttggcattct tggaatttga acaacatcct gttcattttt atagacttgc
     6721 ttaaatggta cagctgaaaa atcaaagtaa tgtccgtttt cgtctggctg tgaaaatgga
     6781 tcaatgagga ttgcggtgta tcttacataa agcagatatt cttgtaagat atctggcaag
     6841 atttcgaaat gctcaatatc accttcatcc aaagtttcag gacgtactgg ataattttcc
     6901 ttcaccataa attgaagcag gttgactgac ggcactctaa atgcttgtaa aatagccatc
     6961 atttccttcc acattaaacg catttcacgg attaatgtca tcgttatcgg tccttcagga
     7021 aataacttat acacataaac cggtacatcc gttccttcga aaatggcttt caatgaaaag
     7081 tcattcataa atagtggcgg gtgcacataa agcgaactgt tacgtgtttc agcatgcagt
     7141 ggcgattcaa cgacttctaa ttgaatcttc aattgctcag ctaaagcaga gattcgttga
     7201 cacattgttg agtttgaatg tgtcgatccc atgtacaatt tcttttttac acctgttgtc
     7261 aacacatgat taggtgcttc tttatcaaca atacgtgtat cgccaagata agttgaaaat
     7321 gaaatcactt cgatatcctg actgaactta gacataaatt gttccacaat catttgcgat
     7381 ccaaatgtcg gtgaaattaa aatgacatgt ttgacacttt gcaaagtttc taacgacaat
     7441 tgctgtagtg tgtcataata agcatctgct gtgcatgcca tgacaaccgt ttcgtattca
     7501 cccttaacgt ttttaacatc tttatacaaa cgattaattt caaacttacc ttccagatgt
     7561 tgatgcgcct cgttttgtat tttgacttca aattgtttct cttttttata cgcttgatat
     7621 aagcgttttg atttttctga tgttgaggca cgtccaacca tatcaatctc ataatctgat
     7681 tttaaatagc aaatattcgc taattggatt gcgaccggac cagtgcctat cattaataat
     7741 ttagacatcc gtaataccta cctttatagc tgctttttta taaagtgcta tatcaaaaat
     7801 ttgctgtggt ctcatatgtt tattcacaca ttgccactta tcttccgctg tttcttgtga
     7861 cggataatta aatattgctt tgatgccatc accaaagcgc attgcaacta cgacattttc
     7921 atttgttaaa tcatataatt cttctaaaat gctgtacttt aaaggaattg tcgagctgaa
     7981 tatgatatgc gtcacatctt tgatatcttt aagttcagat accttttgat ccgtaattgt
     8041 tatatcttca tttggtgcta agacgttaac gattctgcgc cctaggtcaa cggcttgtgg
     8101 atcaatatca ataccgataa ctgaagcacc tgtttctttt gctacttgaa ttaacgtcat
     8161 tggatatgca cctgaaccaa ctaacaataa tttgtcattg ctagtgatac tacattgtcc
     8221 aaactcttca tcaatacaat gttctatatt gtcaaagtaa cctgtggtag acgcctgtcc
     8281 gtctaataga cgtctagctc taatgacttc cacttgttta acacactgcg ctgtaatata
     8341 ttgcagtgat tttattaatt gtgctttttc ttcaacgtca cgccaagcat taaattgttg
     8401 ttcataaata ggatttaaaa taaattcact atagtcatcc atcaatgttt ctaatgcttc
     8461 gatatattga tcgtcttgta atacacgctc gtaatgcgct tcaaactttt ccaaatattg
     8521 ttgtaatatc aatttgattt cattattaaa gttattcatt gtaggctcct tattctatat
     8581 atgcttttcc tgtagctaca gttgtaacct gtcctttaat tgaagtttga tatcccaatt
     8641 gatgacatcg ctttgatgtc actaaaatac tgccccctgg ctgatgtact gtaaaatctt
     8701 tgcatgcgtc attacgttga taattattaa aaaccccaat tgatgctgta ccagaaccac
     8761 agctattttc ccaaattaaa ctttgaattt ctggtatata gattaatggc tgtaaaaatt
     8821 gacgttgttc atcaaaaagc atcataccta ctgttttata tttgtgactc cattgttgct
     8881 cacgcacaaa tgcttcaacc aaatgttgaa tttcagttgt tacttgttta actggaatca
     8941 cataatgtac atatgtttca taaataattt ctaatgcttt ccatgaatga ttacccatat
     9001 taattgttgt tggcacaaca cgatgggctt gtggcatttg aacttcatag tattggcaat
     9061 catgaattgc gcattgcact aaatccgaac agccagatac cttcacctta aactgttggt
     9121 ctttaagcaa atgactttcc tgcaaatgat gtatatatga catcgtcgca ttaccgcaaa
     9181 attcattacc gctcataact aagtgaaaat cattgccatc atcattttgt gttgattcta
     9241 taaagcctac ctgttcacaa catacatgtg ttgcggccat caactgattg gcgatagatg
     9301 catattcact agcatcatgt tttgaatgaa caagtatcgt catattcccc gaaggattat
     9361 acttagaaaa ttctataacc tgccgattca tacgacactc ctttagatgt atttttagag
     9421 cctcttttac cataagtaat tagaggctct tatgcagttg gtgcgaagca agcaactgta
     9481 ttcctctaaa actgtaattt ttagaagatg aacctaatat tttcccatct attaggtaag
     9541 tcatgctaac ccgcaaaagc gaaaaccatg gctcttatgc agttggtgcg aagcaaacaa
     9601 ctgtattcct ctaaaccttg taatttttat aaaaagaacc taatattttc ccatctatta
     9661 ggtaagtcat gctaatccgc atagtggaaa gcatgactta gtaatcggtt gtttcatcaa
     9721 catgcaaccg taaaccttat ttaaagttgc ttcttgtggc tctacaagtg aatgcagttg
     9781 tccgttaact tcattcactt agatgctttt actgttttca cctcaactaa aacttcaaaa
     9841 aagttgcaga aaacaaatcg taactattac gatttgatta tatactatat tagttgagaa
     9901 tcattgtcaa taaaggttgt aaatgaatgc acatttatat tttgataatc tttccataaa
     9961 catcgtattc tcagcacatt caagtctttt gaaaattttt atcaaacaaa aaaaccagct
    10021 tcaacgtaaa atacgttaaa actagcttta agcaatacac ttcatcaaat aaacaaacat
    10081 ttatttttca aaggttaata attccccgtc atttggtaca acaactctat tacctaagtc
    10141 gttagaattt ataaattcat tcagctcttt acgagataaa tgccaatgat tgaccgcttc
    10201 catatgcaca acaacaattt gcgcaagtgg caatgtatta gcaactttta acacatcatg
    10261 ttcatccata actaacggcc caccttcaaa gaattgattt gcaccaccat taagaacaac
    10321 aacatctggt tgatacgttt ttaatgcttt ttcaacccct tcaaaccaaa ctgtatctgc
    10381 tgctaagtat aatgtcggct cttcagaatg ttttaaaatc atgccgtgaa catgtcctgt
    10441 catcttcaaa atttcaccat gaccatgttg agcatctgtt ttaatcaact caatttctcc
    10501 aatgtgtgtc acatcattaa atacagaaac atttgtaaac ccataacgct ctacttcact
    10561 agcatcttct tcattttgtg cgtaaatttt aatatctttc ggcaatactt caattgcttt
    10621 cggatcaaaa tgatcaatat gcaaatgcgt taaaatcact aaatcgatgt catttataat
    10681 gtcttcaatt gccatgggta gttctacaag tggatttttt tgatcttgtc tcggtgaatt
    10741 tggaaaaggg tcaaatgaac ctttatcact taacattgga tcaatcaaaa tgcgtttacc
    10801 tccatactca atcacggccg tggcatttct tatatgtcta atattcatgt taaatccccc
    10861 tatttcatat gtctgtcatt gcgacaatat gcatacattg cctacttcat gccaaattct
    10921 tttccactgg atatcgattt taaataatta ttccaatgtc cgcttccacg aatcatcact
    10981 taacatctcg ccaggccatg tgtaagccat cacaccacca tcaattcgaa tcgtctctcc
    11041 agttatgaat gaactgtcgt cagatgctaa gaagactact aatttagcaa cttcttctgg
    11101 tttacctaaa cgtcccagcg gagtcatcca tttttgattt tctctaaatg ttttacctgc
    11161 atcatcctca ctcgtacctg tcagtttatc tactaacggg gtttcaattg tacctggtgc
    11221 aattgcattg gatcgaatgc catcacgacc atactcaatt gcgattgatt ttgtaaaatt
    11281 aatcactgcg cctttcgcag cattatatcc tgagcgatac aaatcttctg cctgtcctga
    11341 aaatgaagac gtattaacaa tagagccacc ttgattcatc attaaaggta acatcatttt
    11401 cgtcattaaa attgtcccac gcatatctac attcataatc ttgtcataca catctattgg
    11461 atactcatga attctaccag ccgcattatc cacaccggca ttattaaaca acacatcgat
    11521 tcttccaaac tgttccttta tgtcagacac aaagtctacc acttgttgtt catctgaaat
    11581 atccacatta tacgccttcg cattgtcacc gttactttta attttatcga cagtctccga
    11641 taccgcttca gctatgtcta ccgccaatac atacgcacct tcttgagcta aagcgattgc
    11701 agaagcttgt ccgatacctg tacttgctcc tgttacgact gcaactttat tttccaaacg
    11761 tttcattcag tcactcctat acaacatatc ttaaacttac agtccttatt ttccactgtt
    11821 caatgcttca taaacctaat agataagtct ccaggcagtc ttagctactc tctgctatac
    11881 aatttctatt gaataactca aaatatttaa caatattagt cttttcattt tctgaaaatt
    11941 gttacttttg agagtatttt ttgttacact ttaaatattt aacaaagggg ggaatcattg
    12001 atgacatcaa aacatcaaca aaaggggtct atcgtcaatc gtttcttgaa tagtgttgaa
    12061 aaaattggaa ataaattgcc agatcctagc gtcttattct ttttaatgtg tgtgggctta
    12121 gccattatga cgtgggttat ctcattattt aatgtatctg ttaagcatcc aggtacgcat
    12181 caaaccattt atattaaaaa tataattagc catgatggat ttacgatgat aatgaatgat
    12241 acgattaaaa atttctcaga gttcccagca ttaggcttag tactagcagt gatgattggt
    12301 attggtgttg cagagaaaac aggatacttc gataagttaa tgatttctgt tgtgaatcgc
    12361 gcaccacgat ttttaatttt accgactatt atattaattg gtattttagg tagtacagcc
    12421 ggcgatgctg cgacaattat cttgccgccg cttgcagcaa tgctttttat taaaattggc
    12481 tatcacccta ttgctggttt aacgatggca tatgcttccg ctgttggagg atttgcagca
    12541 aatatagttg ttggtatgca agatgctttg gtctattcat ttacagaacc ggcaacacgt
    12601 atcgtttcag attctattaa aacaaacgtt gctatgaact ggtactttat cgcagcgagt
    12661 gtcgttgtat tacttcctac gatattgcta gttacaacta agctcattat tcctagatta
    12721 ggaaaatatg atgacagttt aatgcatgat gaccatgaag aagcatcttc acatattact
    12781 gacaaagaag cacatgcttt aaaatgggca aatatcagtt tcatagtcac aattatttta
    12841 ttaattatta cagctattcc tgaacatagt tttttaagaa atgctaaaac aggcagctta
    12901 ctagacgatg cgccattaat taacggtgtc ggattaatta ttcttgtcgt atttttagta
    12961 cctggattag tttatgggat tttaagcaaa gaaattaaaa atacaaaaga cttaggtaaa
    13021 atgttcggcg atgctgtagg atcaatggga acatttatcg ttattgtgtt ttttgcagca
    13081 cagctacttg cgtatttaaa gtggagtaat ttaggaatta tcgctgctgt taaaggtgcc
    13141 aaattattag aacatcaaaa cggtattgta ttgatactag gtattattgt actgagtgca
    13201 atggtcaaca tgttaatcgg aagcgcatcg gctaaatggg gtattttagg accgatattc
    13261 gtaccaatgt taatccttat cggctttcat cccgcattta cgcaagtcat ttaccgtgta
    13321 ggcgattcaa ttaccaatcc aattacaccg atgatgccgt acttaccttt attattaact
    13381 tatgcacaaa aatacgataa acgcatgaaa ctcggagcct tactttctag tttaatgccg
    13441 tattcaatcg cgctaagcat tgtatggaca gtatttgtca tcatttggtt cttattaggt
    13501 atccctgttg gaccaggtgg accgatattc gttaaataac agtattcaaa gggataaatt
    13561 aaaaatgcca ctatacaaga tttgacctgt atagtggcat gatttgttat gatatagcgt
    13621 tcgagatgaa actctgcatt tatatgaaca attatagtct atcactaata atatttagtc
    13681 tgttccaacc gttaatttgg tttacaacaa agacaagatc aataatttgt tcttcatcat
    13741 aaaatgattt tagccggtca ataatttcaa acttcttaaa gtctttgatt gaatttaaca
    13801 tttctgcaaa ttcaagcgtc actttttctt gatcagtaaa taaatctaaa tgtctaaaac
    13861 tcaagacttc atcaattttc atttgtgtta cacccaattc ctttaattct tttttatgga
    13921 tatcaacaca ataatgacaa ccgttaattt ttgaaattaa taacttaatc aatgcactaa
    13981 gttcatcatt aaccggtgat tgatgaataa acttttcacc ttggctcatt acagacatta
    14041 actctccatt ttgtttcttg taattatacg gcatttgcaa tctctccttt acattgttta
    14101 ataccaatcc agtgatacta cacttcgaac aatattggat agaatttaaa attctgtata
    14161 acatcttgtc aatctatgc
//
LOCUS       DAOBBH010000052        14103 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.052, whole genome shotgun sequence.
ACCESSION   DAOBBH010000052 DAOBBH010000000
VERSION     DAOBBH010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14103
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.052"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..71
                     /locus_tag="P6L01_001156"
     CDS             <1..71
                     /locus_tag="P6L01_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDP3030234.1"
                     /translation="QPRLDYLKAVDKLILDESEDRV"
     gene            68..2251
                     /locus_tag="P6L01_001157"
     CDS             68..2251
                     /locus_tag="P6L01_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDP3030235.1"
                     /translation="MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFF
                     VVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDV
                     PKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLS
                     QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKD
                     LNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDL
                     KANLVNRTPEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTN
                     MDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATD
                     PHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDA
                     LRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQL
                     ASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKP
                     YGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGF
                     SKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQ
                     DNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSGGLTGIFN"
     gene            complement(2817..3158)
                     /locus_tag="P6L01_001158"
     CDS             complement(2817..3158)
                     /locus_tag="P6L01_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030236.1"
                     /translation="MTNITKEVFDNLEQEIDVFAKNKALGSSEAKPYLDEYHSKIIDY
                     FKQVNDITSNIDFDNLNQYPVVPMNFKERYDYMIERKYHFMGYRQMKTFKTELIKMNA
                     SYQTRLKNKQV"
     gene            complement(3163..3822)
                     /gene="nth"
                     /locus_tag="P6L01_001159"
     CDS             complement(3163..3822)
                     /gene="nth"
                     /locus_tag="P6L01_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDP3030237.1"
                     /translation="MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCT
                     DVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFN
                     GEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
                     VEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKE
                     A"
     gene            complement(3812..4498)
                     /locus_tag="P6L01_001160"
     CDS             complement(3812..4498)
                     /locus_tag="P6L01_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HDP3030238.1"
                     /translation="MDKYQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETS
                     NKQPSIELLQKGSTMQPRDITMVIQNLIQRELLELQVQKDEEGRFTEYMNLDPFFEKL
                     SHILKQQSMETKEQNSKEKFKQLFRVLEDTFARPLSPYEIETLNQWIDVDKHDSAIIQ
                     AALDEANSLNKLSFKYMDRILLNWKKNNVKTIDDSRKIREKFNKPKMTHTVKTVPKFD
                     WLNGENLDGK"
     gene            complement(4826..6118)
                     /gene="asnS"
                     /locus_tag="P6L01_001161"
     CDS             complement(4826..6118)
                     /gene="asnS"
                     /locus_tag="P6L01_001161"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDP3030239.1"
                     /translation="MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQ
                     GVVVKSEVDEEVFKLAKEIAQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYP
                     ITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTAS
                     APEGTSELFHTKYFDQDAFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIE
                     FWMIEGEMAFTNHAESLEIQEQYVTHVVKSVLENCKLELKILERDTSKLEKVATPFPR
                     ISYDDAIEFLKAEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNP
                     ENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGS
                     VPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP"
     gene            complement(6440..9133)
                     /locus_tag="P6L01_001162"
     CDS             complement(6440..9133)
                     /locus_tag="P6L01_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase DinG"
                     /protein_id="HDP3030240.1"
                     /translation="MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNQIIDTYHSMI
                     RTNLEIPPFIQALTSIEENMLQQAPYFNQVAQEIYDKIKDCIFVAHNVDFDLNFIKKA
                     FKDCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAHRADEDAATTAK
                     LMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQII
                     YRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQLMHSEKAMIEA
                     SLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALL
                     IKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYF
                     DQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEA
                     HRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDI
                     FGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDLHAIID
                     KLNKTLEIFNGISHKTVKSLRKQLLYLKDKFKNIEQSLKAGHTSFISIKNLSQKSTIR
                     LYVKDYAVKDVLTKQVLEKFKSLIFISGTLKFNHSFEAFKQLFNKDVHFNTFEVNTSL
                     QSAKNTSVFIPSDVASYQYKNIDEYVASIVSYIIEYTTITSSKCLVLFTSYKMMHMVQ
                     DMLNELPEFEDYVVLTQQQNQNYKIVQQFNNFDKAILLGTSTFFEGFDFQANGIKCVM
                     IAKLPFMNKHNAKYWLMDSEFTSTFKEYVLPDAVTRFRQGLGRLIRNENDRGIIVSFD
                     DRLINSNYKNFFEQTLENYRQKKGDIQQFGKLLRQIQKKKK"
     gene            complement(9157..10128)
                     /locus_tag="P6L01_001163"
     CDS             complement(9157..10128)
                     /locus_tag="P6L01_001163"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDP3030241.1"
                     /translation="MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLK
                     LEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSL
                     VGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALG
                     IRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLE
                     NFDESIRHRATSIQLHDKNKLDRYQFLERLLQEIEKRYNQFLTLPFSEIREEYIAASN
                     IWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF"
     gene            complement(10115..11317)
                     /locus_tag="P6L01_001164"
     CDS             complement(10115..11317)
                     /locus_tag="P6L01_001164"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDP3030242.1"
                     /translation="MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDI
                     TTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFV
                     RDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
                     RCLRFQSQLSFDIAMETFEAMRTQMADIKFLSIERIVIELTKLMRGINVEESFNHLKS
                     LKAFNYMPYFEQLDMNQINVTEPIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQ
                     YIQIMNALPSIITKEQLKMFVYDYDTNLIKNVMVAADVLKANDIQGHEPLIVNLQTID
                     ETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILK
                     WVDNHVKI"
     gene            complement(11322..12464)
                     /gene="bshA"
                     /locus_tag="P6L01_001165"
     CDS             complement(11322..12464)
                     /gene="bshA"
                     /locus_tag="P6L01_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HDP3030243.1"
                     /translation="MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIR
                     KPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYDLDLLHMHYAVPHAICGIL
                     AREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEII
                     ETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQVKRIDTIIETF
                     AKVREKIPSKLILLGDGPELVPMRQLTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLL
                     SEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDCDSASDYAIRLLE
                     DKALYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE"
     gene            complement(12708..13025)
                     /locus_tag="P6L01_001166"
     CDS             complement(12708..13025)
                     /locus_tag="P6L01_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDP3030244.1"
                     /translation="MKSMVEMQREVDEYIGQFKTGYFSPLANLARLTEEVGELAREIN
                     HTYGEKKKKDSEEANTIKAELGDNLFVLLCLANSMGIDMTESFNETMEKFNTRDKNRF
                     ERK"
     gene            complement(13361..14041)
                     /locus_tag="P6L01_001167"
     CDS             complement(13361..14041)
                     /locus_tag="P6L01_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDP3030245.1"
                     /translation="MIIYFVILMVLPLWAQHKVKSNYEKYSQVRSTSGKTGREVALEI
                     LHANGIYDVEVVKGEGFLTDHYDPKKKVVSLSPANYDRPSVAGTAIAAHEVGHAIQDH
                     QGYWFLRFRAALVPVANLGSSLSYMIIMLGIILTAIGSAFGSTALWIGAGLMSLAVLF
                     SIVTLPVEFDASSRAMKQITALNIVNEKEYKHARKVLSAAAMTYVAATAVAVAELVRI
                     ILIARSSD"
BASE COUNT         4546 a         2520 c         1974 g         5063 t
ORIGIN      
        1 atcaaccaag attagactat ctaaaagcag ttgataagtt gatattagat gaaagtgagg
       61 accgcgtatg acggaaaaca aaggatcttc tcagcctaag aaaaacggta ataatggtgg
      121 gaaatccaac tcaaaaaaga atagaaatgt gaagagaacg attattaaga ttattggctt
      181 catgattatt gcatttttcg ttgttctttt actaggtatc ttattgtttg cttattatgc
      241 ttggaaagca cctgctttta ccgaagctaa attacaagat ccgattcctg caaagatata
      301 tgacaagaac ggagaacttg ttaaaacatt agataatggc caaagacatg agcatgtaaa
      361 tttaaaagac gtgccgaaat caatgaaaga cgcagtactt gcaactgaag acaatcgttt
      421 ctacgaacat ggcgcacttg attataaacg tttattcggt gcaattggta agaacttgac
      481 tggtggattt ggttctgaag gtgcctcaac attaacacaa caagttgtta aagatgcatt
      541 tttatcacaa cataaatcta ttggacgtaa agctcaagaa gcatacttat catatcgttt
      601 agaacaagag tatagtaaag atgatatctt ccaagtatat ctaaacaaaa tttactattc
      661 tgatggcgta acaggtatta aagctgctgc taagtattac tttaataaag atttaaaaga
      721 tttaaactta gcggaagaag cttatttagc cggtttacct caggttccaa acaactataa
      781 tatttatgat catccaaaag ctgctgaaga tcgtaaaaac actgttttat acttaatgca
      841 ttatcataaa cgcattacag ataaacagtg ggaagatgct aagaaaatcg atttaaaagc
      901 gaacttagta aatcgtactc ctgaagaacg tcaaaacatt gatacaaatc aagattctga
      961 gtataattca tacgttaact ttgtgaaatc tgaattaatg aataataaag cattcaaaga
     1021 tgaaaattta ggtaatgtat tacaaagtgg tattaaaatt tatacaaaca tggataaaga
     1081 tgttcaaaaa acattacaaa atgatgttga taatggtagc ttctacaaga ataaagacca
     1141 acaagttggt gcaacgattc ttgatagtaa aactggtggt ttagttgcta tatctggtgg
     1201 acgtgatttc aaagacgtcg ttaacagaaa ccaagcaaca gatcctcacc ctactggttc
     1261 atctttaaaa cctttcttag cgtatggacc tgccattgaa aatatgaaat gggcaacaaa
     1321 ccatgcgatt caagatgaat cttcatatca agttgacggt tctacattta gaaactatga
     1381 tacgaagagt cacggtactg tatctattta tgatgcttta cgacaaagtt tcaatatccc
     1441 agctttaaaa gcatggcaat cagttaagca aaatgctggt aacgatgcac ctaagaaatt
     1501 cgctgccaaa cttggcttaa actacgaagg cgatattggt ccatctgaag tacttggtgg
     1561 ttctgcttca gaattctcac caacacaatt agcatcagca tttgctgcaa tcgctaacgg
     1621 tggtacttat aacaacgcgc attcaattca aaaagtagtt actcgtgacg gtgaaacaat
     1681 cgaatacgat catactagcc ataaagcgat gagtgattac actgcataca tgttagctga
     1741 gatgctaaaa ggtacattta aaccatatgg ttctgcatat ggccatggtg tatctggagt
     1801 aaatatgggt gctaagacag gtactggtac ttacggtgct gaaacttatt cacaatataa
     1861 tttacctgat aatgcagcga aagacgtgtg gattaacggc tttacacctc aatacactat
     1921 gtcagtgtgg atgggcttca gtaaagttaa acaatatggt gaaaactcat ttgtgggaca
     1981 tagccaacaa gaatatccac agttcttata tgaaaatgtg atgtcaaaaa tttcatctag
     2041 agatggcgaa gactttaaac gtcctagctc agtaagtggt agtatcccat caatcaatgt
     2101 ttctggtagt caagataaca acactacaaa tcgtagtaca cacggtggta gtgacacatc
     2161 agcaaacagc agtggtactg cacaatcaaa taacaatact agatctcaac aatctagaaa
     2221 cagcggtgga ttaacaggta tattcaacta atccactcaa cataaaatcc tcagttatac
     2281 catatttatg gtgtagctga ggattttttt aggttcttca tcttttatgg tgggaaggta
     2341 aaacttcctg ctttttttat aagtattcat acgctaatgc tatattagta gaaaccgtaa
     2401 aatcagtcat tcacatacat tttgaaacat ctaaatagtt tttcatattc acatctttag
     2461 ctatgtaaaa ttattgaatt tgaataagtg gttgttccca tttttaaaat tatgattata
     2521 ttttctcata gtcatcttag acgctgtttt aaattgaaat gtttatcaat tccctgtgta
     2581 cgttaatcag tgaaatatca agatgtttgc aacatatttt cacaatcata gatataagaa
     2641 atccacctac gattcgaaat aggtatctta aagcatatct caagatatcc attatgatgt
     2701 agatggacag tttttatatt aagacgttgt aatgacacga cacctaacat aaatacaaat
     2761 atgtgtgcat gaattagttt tacatatttt caacattaag aaatgatcaa tcttgtctat
     2821 acctgcttat tctttaacct tgtttgataa ctagcattca ttttaatcaa ttcagtttta
     2881 aaggtcttca tttgtcgata gcccataaaa tgatatttac gttcaatcat ataatcatat
     2941 ctttctttaa aattcattgg cacaacagga tattgattta aattatcaaa atcaatattg
     3001 ctagttatgt cattaacctg cttaaaataa tcaataattt tactatggta ttcatccaag
     3061 tagggctttg cttcactaga acctaatgcc ttatttttgg caaatacatc aatctcttgt
     3121 tctaaattat caaatacctc ttttgtaata tttgtcatgt tatcacgctt ctttcaaact
     3181 agctttataa cgtttttgtc cttctctaca atcttctaat agtggacaaa tatcgcattt
     3241 aggttttcgg gctaaacagt ggtatcttcc aaagaaaatg agttgatgat ggctcctatt
     3301 ccatctatct ctaggtatga cagaacataa acggtcttct acctgtctca cattatcttt
     3361 ccaacgatta atacctaagc gtttagaaac acgttctaca tgcgtatcaa cagctaatga
     3421 aggttcatca aatgctacac tcatgactac attagcagtt ttacgtccta cacctgctaa
     3481 actttctaat tccttatgtg tttgtggtat ttctccatta aattgatcaa tcaaagattg
     3541 acaaagtttc ttaatattct tagctttgtt acgatacaga ccgatagaac gaatatcatt
     3601 cataagttct tcatcactga ctgccaaata atcttcaggc gttttgtatt ttttaaacag
     3661 ctcagttgtt actctattta ctagaacgtc tgtacattgc gctgacaata atacagcaat
     3721 agttaattcg aacggattat catgttttaa ttcacattct gcatccggaa acatatttgc
     3781 tataacatca atcatttcta atgctttttt cttacttacc atcaaggttc tccccgttta
     3841 accaatcaaa tttaggtacc gttttaactg tgtgcgtcat tttcggttta ttaaattttt
     3901 ctcttatttt tctagaatcg tcaattgttt tgacattgtt tttcttccaa ttaagtaaaa
     3961 tacgatccat atatttaaag ctaagtttat tcaaactatt cgcctcgtct aatgccgctt
     4021 gtataattgc actatcgtgt ttatcaacat caatccattg atttaacgtt tctatttcat
     4081 atggagataa cggccttgca aatgtatcct ctaaaactct aaataattgt ttaaattttt
     4141 ctttactatt ttgctctttc gtttccatac tttgttgctt caatatatga cttaattttt
     4201 cgaaaaaagg atctagattc atatattcgg taaatctacc ttcttcatct ttttgaactt
     4261 gtaattctag caattcacgt tgtatcaaat tttgaataac cattgtaata tcacgtggtt
     4321 gcatagttga gcccttctga agtaattcaa ttgaaggctg tttatttgat gtttcggaag
     4381 cataaatcaa tttaagcaaa atgactaaat cttgctcatc taaacctaag tcactgtaat
     4441 ggtctaataa ttctcttcgt atcactacag gtcttgcttt taattgatat ttatccatgt
     4501 tcgtgccccc ttttaaattt aaccattact taatagtata aatcattttc tttaattttc
     4561 gactaaaatg cttaatatat ttaactagta gcaaacaaat tttaaataac agtatttttt
     4621 aaaactagat tttaacaatg aactttatgg ataatcagat gaactattta ttttccaaat
     4681 atagttacta aacaaaagtt tgttcatgat taatataagc cgttatcaat cttcattttg
     4741 caagcataaa tcgcccagct attaatatag gctggacgat tctttttatt atcataatat
     4801 aaattttaag taacgatcct aaaacttatg gatataaacg gtttaataat cttgggaatg
     4861 gcgcagtttc acgaacgtgt tcaacaccag aaatccatgc tactgtgcgc tctaaaccta
     4921 aaccaaatcc acagtgtggc acactaccat aacgacgtaa gtctaagtag taactatatg
     4981 cttcttcgtc taatccatgt tctttaacgc gttgttctaa caattctaag tcatccacac
     5041 gttcagatcc accgataatt tcaccgtatc cttcaggtgc aattaagtct gcacataaga
     5101 cagtttcttc attctcagga tttggttgca tatagaaagg cttaatttta gttggataat
     5161 tagtaataaa caccggtaaa tcataatgat tagcaatggc agtttcatgt ggcgcaccaa
     5221 aatcttcacc ccattcaata tcatcaaagc cttctgcttt taagaattca attgcatcat
     5281 catatgaaat tctagggaat ggtgtcgcaa ctttttcaag ttttgatgta tcacgctcta
     5341 aaattttcaa ctctagttta caattttcta aaactgattt tactacatgt gttacatatt
     5401 gttcttgaat ttctaaactt tcagcatgat ttgtgaaagc catttcccct tcaatcatcc
     5461 agaactcgat caagtgtcta cgtgtttttg atttttcagc tctgaaagtt ggaccaaatg
     5521 aaaatacttt tccgtgtgcc attgctgcag cttctaagta taactgacca ctttgagata
     5581 aaaacgcatc ttgatcaaag tatttagtat ggaataattc acttgtacct tctggtgcac
     5641 ttgctgtcaa aattggtgga tcaacctttg taaatccatc tttgttgaaa aattcatacg
     5701 ttgcacgaat aacttcattt ctaattttca ttacagcatg ttgtttttta gaacgtaacc
     5761 ataaatgacg gtgatccatt aagaattctg taccatgatt tttaggtgta atcggatagt
     5821 catgcgcttc tgaaataact tcaattgatt tcacttgcat ttcgtatcct aagtcagaac
     5881 gattatcttc tgtaattgtg cctgtaacgt atagagatga ttcttgagca atttctttcg
     5941 caagtttgaa tacctcttca tcaacttctg atttaactac tacgccttgc ataaagcctg
     6001 ttccatcacg taattgtaaa aaggcgattt taccacttga acgtttattt gttaaccaag
     6061 caccaattgt aacgtcttgg tttaaatgat ctttcgcttg tttaatcgtt gttttcataa
     6121 ccattctcct atttattttt tcgttataca atactcattc attttaacaa aatccgcttt
     6181 caagttctag aactagacta aaagataacg tgtaaatggt aatgatttac gcacagattt
     6241 gaacattaat ttatattaag acaatacaat catcagtttc agacaaaata tataataaag
     6301 cctcaatatt attagtatga aggtcgtttt ttgtgctaac attcaagttt aattataaaa
     6361 ctcaaatttt gacgaccaaa caaaaattct tgtaacagaa catagcacat gtcatgtcac
     6421 aagaatttta ctttgtattt cactttttct ttttttgaat ttgtcttaat aattttccaa
     6481 actgttgaat gtcgcctttt ttctgacgat aattttcaag tgtttgttca aaaaagtttt
     6541 tataattact gtttatgagt cgatcatcaa atgaaactat tatgccgcga tcattttcat
     6601 ttctaattaa tcttccaagt ccttgtctaa aacgtgtaac tgcatcaggt aatacatatt
     6661 ccttgaaagt tgaagtgaat tcagaatcca taagccaata ttttgcatta tgcttgttca
     6721 taaacggtaa cttcgctatc atcacacatt taataccatt tgcttgaaaa tcaaaacctt
     6781 caaaaaatgt tgacgtacca agcagtatgg ccttatcaaa attattaaac tgttgtacta
     6841 ttttataatt ttggttctgc tgttgtgtta atacaacata atcttcaaat tctggcaatt
     6901 catttagcat atcttgtacc atatgcatca ttttataact cgtaaataag actaaacatt
     6961 ttgatgacgt tatagtcgta tattcaataa tatagcttac aatcgatgct acatactcat
     7021 ctatattttt atattgataa gatgctacat cactcggtat aaatacactt gtattttttg
     7081 cactttgtaa cgacgtgtta acttcaaatg tattaaagtg aacatctttg ttgaataact
     7141 gtttaaaagc ttcaaacgaa tgattaaatt ttaaagtacc agatataaaa atgagtgatt
     7201 taaatttttc caatacttgt ttcgttaata catcttttac agcgtaatct ttcacatata
     7261 aacgtattgt tgatttttga gataaatttt taattgaaat gaaactagta tgtccagctt
     7321 ttaaactttg ttcgatattt ttaaatttat cttttaaata caacaactgt ttccgtaatg
     7381 atttaactgt tttatgacta atgccattga atatttctag cgttttattt aacttatcga
     7441 taatcgcatg taaatccttc aaaatgtctt ttgtttcaaa agtaaataca ttatggaagc
     7501 gatgaatatc atcatcataa acatcagaat cattgataat cgtaaatatc gttgagaaca
     7561 attgctcatt taactcatga atctcattca tactagcctt caagccaaaa atatcaattg
     7621 gtgcaatatc taatttttcc aaaattcgct gcttttccag ttgatcgatt gcctttaaca
     7681 atttttcatt ttcgttttta ccaatcaaac caagctgata tttaatatca gcataactca
     7741 actcatttgt cacttgattt aaggcatagt ctggtaagcg atgtgcttca tccactatac
     7801 aatcatcaaa caattgatat attgaatttt caacatcaga atgaattaaa tgtgcatgat
     7861 ttgtaatacc aatttgaatg ttctgtgcat ttcgcttaat aaaattataa taatgaacat
     7921 cgtgacgtgc cggtacatat gtttcaattt tctggtcaaa atacatcttt tgaccacctt
     7981 ttaaatttaa ttcctgtata tctccggacg gcgtctctgt aatccaaatt aacaattgca
     8041 ttttcaagat attcacttcg taattacttg tgtcatcttt taaaatttga ctaataagtc
     8101 ccaatgaaat gtaatcactt ttacttttaa tcaatagtgc attaatttta aaatttaacg
     8161 cttcattcat tgctggaata tctttttcta acaattgact ttgcagtaat ttagtattgg
     8221 tagaaatcat gacatgcttc ccagtttcaa tattatacat caaggccgca agtaaatatg
     8281 ctaatgattt accactgcct agtgatgctt caatcattgc tttttcacta tgcatgagct
     8341 gatctaatat agtttccgct aaatataatt gttgcggtcg atatgttaag ccaagttgat
     8401 ctacagcttt gctatataaa gacttcaagc tgccattata atttgttgtc ggctttttaa
     8461 aatcaacttg cttacgatag ataatctgtt cgaacttttc gtacgattta tccaatggct
     8521 ttgcatcata ttgcctaacc atctcaaaga aaatatcata caaatcgtat ttcaactgtt
     8581 tacttaaata atataattgc ttcaaagtat ctaacggtaa cttttcaaat ttttcaaaag
     8641 ctaatatcat caatttagca gtagtagcgg catcttcgtc agctcgatgg gcatttgcta
     8701 aggtaatacc atgtgcctct gctaattcac ttaattgata gcttttatct gtaggaaaag
     8761 ctattttaaa gatttctagt gtatctataa cttttttggg acgatattga atattacaat
     8821 ctttaaatgc ctttttaata aaattcaaat caaaatctac attatgagcg acaaaaatgc
     8881 aatcttttat cttatcgtag atttcttgtg caacttgatt aaaatatggc gcttgttgta
     8941 gcatattttc ttcaatggat gttaacgctt gaatgaacgg cggaatctct aaatttgttc
     9001 taatcataga atgatatgta tcaataattt ggttattgcg cacaaacgtt ataccaattt
     9061 gaatgatatc gtcaaaatct aattggttgc ctgttgtttc caaatccaca acggcatagg
     9121 ttgccatacc catagctatc tctccttgct ttagtgttaa aaatctatat ctgcactaat
     9181 taaacggtgt gattcacccg cttcatctct aacaattaga tagccatcgt aatctaaatc
     9241 aattgcttgt cctttaaact gtttatcatt ttctgtaaat agcaacgttc tattccaaat
     9301 attagaagct gcaatatatt cttcacgaat ttcagaaaaa ggtaacgtta aaaattgatt
     9361 atatcttttt tcaatttctt gaagtaatct ctctaaaaat tgatatctat ctaatttatt
     9421 tttatcatgt aattgtatac ttgttgctct atgtctaata ctttcatcaa agttttctag
     9481 ttgttgcgtc aaattaatac ctataccaca tattattgct tctataccat cattattagc
     9541 aaccatttca gttaagaaac cacacacttt accattatca atatatatat cattcggcca
     9601 tttcactttg acttcatctt gactaaaatg ttgaatcgca tctcttatcc ctaatgcaat
     9661 aaataaatta aatttagata tcattgagaa tgcaacgtta ggtcttaaca cgacagacat
     9721 ccaaagtcct tgcccttttg aagaactcca atgtctatta aatcgcccac gacctttcgt
     9781 ttgttcatca ctcaagataa aaaatgaaga ttgatttcca acaagtgact ttttcgcagc
     9841 aagttgtgta gaatctattg aatcgtatac ttcactaaaa tcaaacaaag cagaactttt
     9901 tgtatattgg tctattatac cctgatacca aatatctggg agttgttgta ataaatgccc
     9961 tttatgattt actgaatcaa ttttacatcc ctctaacttt aattggtcaa tcaccttttt
    10021 tactgcagtg cgtgaaatat taagtgattc cgcaatgctt tgtccagata tataattcgg
    10081 tttattttta tagagtaatt gaagtacatc ttgactatat tttgacatga ttatccaccc
    10141 atttcaaaat ttcagtttct tcgttgctta ctttacctgt tacaatcgct atctcaattt
    10201 gtcttagcac atcttttaac cacggaccac ttttggcatt taaatgtgcc ataagtacac
    10261 cgccattaac catcatgtct tttctattat gcataggtaa acgatgtaat gtttcatcaa
    10321 tcgtttgaag gttaacgatt aatggttcat gtccttgaat atcatttgcc tttaacacgt
    10381 ctgctgcaac cattacattt ttaatgagat tcgtatcata atcataaaca aacattttta
    10441 attgttcttt tgtaataata cttggtaatg cattcataat ttgaatatat tgattgatat
    10501 cttttacttg tcggttactt agctttaaag gcttcaatga gtaattaata tcaaatttaa
    10561 ctgacactat agcaatcaac aattctaaat caattggttc agttacatta atttgattca
    10621 tatcaagttg ttcgaaatat ggcatataat taaatgcttt cagcgatttt aaatgattaa
    10681 aactctcttc aacattaata cctcgcatta atttagttag ttcaatcact atacgctcaa
    10741 ttgataaaaa tttaatatct gccatttgtg tacgcatcgc ttcaaatgtt tccattgcaa
    10801 tatcaaatga taattgtgac tggaatctta aacatcgaat catacgtaaa gcatcttctt
    10861 ggaatcgttc ctcagctata cctacagttc ttattattcg attattaata tcttgttgac
    10921 catcaaaata atcatacaat ttgtatgctg tatccattgc tatcgcattc atcgtgaaat
    10981 ctcttcgttg caaatcttcg tataaatccc gaacaaatgt aacaccactt ggtctacggt
    11041 gatcgacata atcttcttca gcccggaatg ttgtcacttc ataattttca tcattaaaaa
    11101 ctacatttat ggtgccatgt tctttaccta caggtatcgt atgactaaag atagattcta
    11161 tttcatccgg cgttgcactt gttgtgatat ctatatcatg aatatttctt cccatgacat
    11221 aatctcttac agagccacct acataatatg cttcaaaacc attgtcttga atttgttcta
    11281 atataggcct tgcctgttca aataatgatt tatccatatt attactcgcc tttacttttg
    11341 ttatgctcat ttagcatttt ttgataataa tactcatatt gatctgtaat aagttctgat
    11401 ccaaaacgtt cagcaatatc tgctagcatg tttttctgaa gtttgttgta taacgcctta
    11461 tcttcaagta aacggatagc atagtcactc gctgaatcac aatcacccac atctacaaca
    11521 aatccagttt caccatgttt aataacctct ttaattccac cggcatttga accaattgga
    11581 acgacgcctg ttttcatagc ctcaagtaaa gttagtccaa agctttcttt ttcacttaat
    11641 aataatacta agtcagataa ttgataaaat tcacttacac aatcttgttt ccctaaaaat
    11701 aaaacatcct cttctacgtt taactctttc gtcaattgac gcattggcac taattcagga
    11761 ccatctccaa gtaaaattaa tttactaggt atcttttcac gtacttttgc aaatgtttct
    11821 ataatagtat ctatgcgttt tacttgtcta aaattcgata catgtattaa cactttttca
    11881 tctggtgcta taccaaattg tgattttaat gctgtgttat gtttagttgg aaactcattt
    11941 tcacgtacaa aattataaat cggtataatt tctttgttag tttcgataat ttcatgtgtt
    12001 tcttgcgcta aagatttact cacacttgtc acaatatcac ttttttcaat gccaaattta
    12061 attgcacctt ggagtgaatg atcatagccc aaaacagtaa tatcagtacc gtgtagcgtt
    12121 gtcataattt ttatatcttt acctgacatc tcacgagcta aaattccaca aattgcatga
    12181 ggtacagcat agtgcatatg caacaaatca agatcatatt ctttaataac ttcagcgatt
    12241 ttagtactta acgtaatatc atacggtgga tattgaaata ctgcatattg attcacttca
    12301 acttgatgaa aaatcatatt cggcaatggt tttcttattc taaacgggat atttgaagtg
    12361 ataaaatgta cttcgtgacc tcgctctgct aatttaattc ctaattctgt ggcaataatt
    12421 ccagaaccac ccatggacgg gtaacatgtt atacctatct tcattcgctt gcccatcctt
    12481 tctttctatt tctctttcta ttattctcga tgcgtagata attgtttaaa tttaagttta
    12541 tagtaatgtt gagtttataa tttcaaatat ctaaaaaaca ggtgttgtat atataatcat
    12601 ttatctagtt ctacttactt taaaaataat ataatttcat gcgatgcaat tcattggtct
    12661 atgtttttaa tcttaatcaa atccaaataa agcatatatt tttaaattca ctttctttcg
    12721 aatcgatttt tatctcttgt attaaacttt tccattgttt cattaaagct ctctgtcata
    12781 tctattccca ttgaattcgc taaacataac aacacaaata aattatcacc taattctgct
    12841 ttaatcgtat ttgcttcctc tgaatctttc ttcttttttt caccataggt atgatttatt
    12901 tcacgtgcaa gttcgcccac ttcttcagtc aatctagcta agttagctaa tggtgaaaaa
    12961 tatcctgttt taaattgtcc aatatattca tcaacttcac gttgcatttc taccattgat
    13021 ttcatttcta cgttctcctt atattgcatt tctaatatag tatatatcaa tttgaagtct
    13081 catgcatgtt tagtgcaatc tactgactct agctaaacta gatgacaagt tgtttatttt
    13141 agaagtttaa taattcagtt tatataaatg taatgcattc ctaactaaat taaatcaatt
    13201 gaaattggga ttataacttt atgattcgta ccactacaat aaaataatat aatgaataat
    13261 ctaccattag aaaaataagc acaaaaaaac tagcaatcac acaaaatgtg attagctagt
    13321 taataagtgt ctaatttaag ttaattgtta atctataaga ttaatcactt gaacgcgcaa
    13381 tcaaaataat acgtacaagc tctgctacag cgacagcagt tgctgcaaca taagtcattg
    13441 ctgctgcaga taatacttta cgcgcatgct tgtattcttt ttcatttaca atgttcaatg
    13501 ccgtaatttg tttcatcgct cttgaactcg catcaaactc aactggtaac gtaacaattg
    13561 agaataatac cgctaatgac attaaaccag caccaatcca taaagcagtt gaaccaaatg
    13621 cactacctat cgctgttaag ataataccta acatgatgat catataactt aatgaactcc
    13681 ctaggtttgc aacaggtact aatgctgctc tgaatcttaa gaaccaatat ccttggtgat
    13741 cttgaatggc atgaccaact tcgtgggctg caattgcagt tccagcaact gatggtctgt
    13801 catagtttgc aggagatagt gaaacaactt tctttttagg atcgtaatga tctgttaaga
    13861 atccttcacc tttaacaact tcgacatcat aaataccgtt tgcatgtaaa atttctaatg
    13921 caacttcacg acccgtttta ccactagttg atctaacttg tgaatatttc tcatagttag
    13981 atttaacttt gtgttgtgcc cataaaggaa gcaccattaa tattacgaaa taaattatca
    14041 tagtaaaaat tgaagacaat aaactcactc tcctttataa atattttact gtcatttgcc
    14101 gtt
//
LOCUS       DAOBBH010000053        14069 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.053, whole genome shotgun sequence.
ACCESSION   DAOBBH010000053 DAOBBH010000000
VERSION     DAOBBH010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14069
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.053"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            108..1016
                     /locus_tag="P6L01_001168"
     CDS             108..1016
                     /locus_tag="P6L01_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDP3030246.1"
                     /translation="MKKLLLPLIIMLLVLAACGNQGEKNNKAETKSYKMDDGKTVDIP
                     KDPKRIAVVAPTYAGGLKKLGANIVAVNQQVDQSKVLKDKFKGVTKIGDGDVEKVAKE
                     KPDLIIVYSTDKDIKKYQKVAPTVVVDYNKHKYLEQQEMLGKIVGKEDKVKAWKKDWE
                     ETTAKDGKEIKKAIGQDATVSLFDEFDKKLYTYGDNWGRGGEVLYQAFGLKMQPEQQK
                     LTAKAGWAEVKQEEIEKYAGDYIVSTSEGKPTPGYESTNMWKNLKATKEGHIVKVDAG
                     TYWYNDPYTLDFMRKDLKEKLIKAAK"
     gene            1414..2355
                     /locus_tag="P6L01_001169"
     CDS             1414..2355
                     /locus_tag="P6L01_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDP3030247.1"
                     /translation="MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEP
                     SLSASVKIINRFSNEDIQVAPSYERGKNPFPKEWRMHAFFMDALPILNEPVKHVASNV
                     SDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPK
                     GNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANER
                     QYTGIDFLGVSYAAVPPLTHFITNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGP
                     SQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN"
     gene            2476..3030
                     /locus_tag="P6L01_001170"
     CDS             2476..3030
                     /locus_tag="P6L01_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDP3030248.1"
                     /translation="MTTKQLVYTALMTAIIAILGMVPVIPLPFSSVPIVLQNIGIFLA
                     GVILGRKYGTLSVIVFLLLVVAGLPLLSGGRGGIGVFAGPSAGFLLLYPVVAFMIGAI
                     RDRFINEINFWILFVGILVFGVIALDVIGTLIMGMIINIPFTKAISISLAYLPGDILK
                     AIVASLIGTALLNHSQFRQIMGLK"
     gene            3107..3877
                     /locus_tag="P6L01_001171"
     CDS             3107..3877
                     /locus_tag="P6L01_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDP3030249.1"
                     /translation="MASISMSDIYCNGTIFENDDEQLIYLTPSFPQRYTSNTWIYKKT
                     PTQERWLKDLERQHQLHTNQGSNHYAFSFPENEQLDNHWMAMFKDMNFELGIMELYAI
                     ESDALANLPRNSDVEIAVVDESHIDAYLKVAYQFSLPFGKDYADAHEEMVREHYQKDV
                     IKRLVAYLNNEPIGVVDVIESENYIELDGFGVLEQFRHQGIGSTIQSLIGEYAISKNH
                     KPIILVTDGEDTAKDMYAKQGYVYQSFCYQILKEDIGN"
     gene            complement(4049..4846)
                     /gene="fdhD"
                     /locus_tag="P6L01_001172"
     CDS             complement(4049..4846)
                     /gene="fdhD"
                     /locus_tag="P6L01_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDP3030250.1"
                     /translation="MNKDVSLGQPIVRYEDGKLFNTTDQYVTEFPLTIMVNGEEFATV
                     ICSPTNLEELVIGFLASEGAILKRDELKSVLIDDSKGFAHVELNKDLGDRFQYSTKRM
                     IASCCGKSREFYFQNDAAIAKTSMSKITLTPIQIINMMTRLQSASHIYQETGGLHNAA
                     ISDGLTFFVHRQDIGRHNALDKLYGFCIQRHITVRDKVLIFSGRISSEILIKAAKIGV
                     GVILSKSAPTTLAVTLANDLNITAVGFIRNGGFNIYSHPERIIDSEQ"
     gene            5135..5917
                     /gene="modA"
                     /locus_tag="P6L01_001173"
     CDS             5135..5917
                     /gene="modA"
                     /locus_tag="P6L01_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDP3030251.1"
                     /translation="MKMKRFIAIVMALFLVLAGCSNSNDNNESKKDDADNGKKQEIQV
                     AAAASLTDVTKKLASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVD
                     ALKDKNKAHDTYKYAKNSLVLIGDKDSNYTSVKDLKDNDKLALGEVKTVPAGKYAKQY
                     LDNNNLFKEVESKIVYAKDVKQVLNYVEKGNAKQGFVYKTDLYKQNKKIDTVKVIKEV
                     ELKKPITYEAGATSDSKLAKEWMEFLKSDKAKEILKEYHFAA"
     gene            5931..6602
                     /gene="modB"
                     /locus_tag="P6L01_001174"
     CDS             5931..6602
                     /gene="modB"
                     /locus_tag="P6L01_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDP3030252.1"
                     /translation="MPDLTPFWISIRVAVISTIIVTVLGIFISKWLYRRKGSWVKVLE
                     SLLILPIVLPPTVLGFILLIIFSPRGPIGQFFANVLHLPVVFTLTGAVIASVIVSFPL
                     MYQHTVQGFRGIDTKMINTARTMGASETKIFLKLILPLAKRSILAGIMMSFARALGEF
                     GATLMVAGYIPNKTNTLPLEIYFLVEQGRENEAWLWVLVLVAFSIVVISTINLLNKDK
                     YKEVD"
     gene            6603..7208
                     /locus_tag="P6L01_001175"
     CDS             6603..7208
                     /locus_tag="P6L01_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDP3030253.1"
                     /translation="MLKINVKYQLKNTLIRINIDDTEPKIYAVRGPSGIGKTTVLNMI
                     AGLRKADEAIIEVNGQLLTDTTKNVNVKIQQRRIGYLFQDYQLFPNMTVYKNITFMAE
                     PSKHIDQLIQTLNINHLMKQYPMTLSGGEAQRVALARALSTKPDLILLDEPFSSLDDT
                     TKDESITLVKRIFNEWQIPIIFVTHSNYEAEQMAHEIITIG"
     gene            7378..8382
                     /locus_tag="P6L01_001176"
     CDS             7378..8382
                     /locus_tag="P6L01_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HDP3030254.1"
                     /translation="MSQERYSRQILFKQIGEIGQSKINQKCALIIGMGALGTHVAEGL
                     VRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDGC
                     IAHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKHRIPWIYGGVVQSTYTEAAF
                     IPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITY
                     GDIWEGSHYSFGFSKMQRSDCTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENASITH
                     DILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG
                     "
     gene            8413..8919
                     /locus_tag="P6L01_001177"
     CDS             8413..8919
                     /locus_tag="P6L01_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaB"
                     /protein_id="HDP3030255.1"
                     /translation="MGEHQNVKLNRTVKAAVLTVSDTRDFVTDKGGQCVRQLLQADDV
                     EVSDAHYTIVKDEKVAITTQVKKWLEEDIDVIITTGGTGIAQRDVTIEAVKPLLTKEI
                     EGFGELFRYLSYVEDVGTRALLSRAVAGTVNNKLIFSIPGSTGAVKLALEKLIKPELN
                     HLIHELTK"
     gene            complement(8921..9415)
                     /gene="moaC"
                     /locus_tag="P6L01_001178"
     CDS             complement(8921..9415)
                     /gene="moaC"
                     /locus_tag="P6L01_001178"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDP3030256.1"
                     /translation="MTEFTHINQQGHAKMVDVSDKQITKRTAVAHSSITVNETIFKQI
                     SNNTNTKGNVLNTAQIAGIMAAKNTSTIIPMCHPLPLTGIDVHFSWDETNAPLYTLNI
                     QTTVSTTGKTGVEMEALTAASATALTIYDMTKAVDKGMIIGETYLESKSGGKSGDFQR
                     QSGQ"
     gene            9478..10737
                     /locus_tag="P6L01_001179"
     CDS             9478..10737
                     /locus_tag="P6L01_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDP3030257.1"
                     /translation="MVVEKRNPIPVKEAIQRIVNQQSSMPAITVALEKSLNHILAEDI
                     VATYDIPRFDKSPYDGFAIRSVDSQGASGQNRIEFKVIDHIGAGSVSDKLVGDHEAVR
                     IMTGAQIPNGADAVVMFEQTIELEDTFTIRKPFSKNENISLKGEETKTGDVVLKKGQV
                     INPGAIAVLATYGYAEVKVIKQPSVAVIATGSELLDVNDVLEDGKIRNSNGPMIRALA
                     EKLGLEVGIYKTQKDDLDSGIQVVKEAMEKHDIVITTGGVSVGDFDYLPEIYKAVKAE
                     VLFNKVAMRPGSVTTVAFADGKYLFGLSGNPSACFTGFELFVKPAVKHMCGALEVFPQ
                     IIKATLMEDFTKANPFTRFIRAKATLTSAGATVVPSGFNKSGAVVAIAHANCMVMLPG
                     GSRGFKAGHTVDIILTESDAAEEELLL"
     gene            10734..11219
                     /gene="mobB"
                     /locus_tag="P6L01_001180"
     CDS             10734..11219
                     /gene="mobB"
                     /locus_tag="P6L01_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDP3030258.1"
                     /translation="MILQIVGYKKSGKTTLMRHIVSFLKTHGYTVATIKHHGHAKEDI
                     QLQDSDVDHMKHFEAGADQSIVQGFQYQQTVTRVDNQNLTQIIEKSVTIDTNIVLVEG
                     FKNADFEKVVVYRDEEELQVLQQLSNVCYSINVREHEDFTAFEQWLLNKIKNDCDTQL
                     T"
     gene            11233..11679
                     /locus_tag="P6L01_001181"
     CDS             11233..11679
                     /locus_tag="P6L01_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HDP3030259.1"
                     /translation="MKQFEIVIEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKT
                     EYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRK
                     DAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE"
     gene            11685..11918
                     /gene="moaD"
                     /locus_tag="P6L01_001182"
     CDS             11685..11918
                     /gene="moaD"
                     /locus_tag="P6L01_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HDP3030260.1"
                     /translation="MKVLYFAEIKDILQKAQEDIVLEQALTVQQFEDLLFERYPQINN
                     KKFQVAVNEEFVQKSDFIQPNDTVALIPPVSGG"
     gene            11925..12524
                     /gene="mobA"
                     /locus_tag="P6L01_001183"
     CDS             11925..12524
                     /gene="mobA"
                     /locus_tag="P6L01_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HDP3030261.1"
                     /translation="MKAIILAGGHSVRFGKPKAFAEVNGETFYSRVIKTLESTNMFNE
                     IIISTNAQLATQFKYPNVVIDDENHNDKGPLAGIYTIMKQHPEEELFFVVSVDTPMIT
                     GKAVSTLYQFLVSHLIENHLDVAAFKEDGRFIPTIAFYSPNALGAITKALHSDNYSFK
                     NIYHELSTDYLDVRDVDAPSYWYKNINYQHDLDALIQKL"
     gene            12544..13566
                     /gene="moaA"
                     /locus_tag="P6L01_001184"
     CDS             12544..13566
                     /gene="moaA"
                     /locus_tag="P6L01_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDP3030262.1"
                     /translation="MVEQIKDKLGRPIRDLRLSVTDRCNFRCDYCMPKEVFGDDFVFL
                     PKNELLTFDEMARIAKVYAELGVKKIRITGGEPLMRRDLDVLIAKLNQIDGIEDIGLT
                     TNGLLLKKHGQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYATSIGL
                     NVKVNVVIQKGINDDQIIPMLEYFKDKHIEIRFIEFMDVGNDNGWDFSKVVTKDEMLT
                     MIEQHFEIDPVEPKYFGEVAKYYRHKDNGVQFGLITSVSQSFCSTCTRARLSSDGKFY
                     GCLFATVDGFNVKAFIRSGVTDEELKEQFKALWQIRDDRYSDERTAQTVANRQRKKIN
                     MNYIGG"
     gene            complement(13832..14017)
                     /locus_tag="P6L01_001185"
     CDS             complement(13832..14017)
                     /locus_tag="P6L01_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030263.1"
                     /translation="MFRFISFGQLVVYSTTFIPKKDILQLNDLECDINVKEPLKINYV
                     VIFGSGTEITFSQNLFR"
BASE COUNT         5001 a         1948 c         2733 g         4387 t
ORIGIN      
        1 aattttttgc gaaaataaaa aaataaatag cactagctat tgtaaatgta tatgcaatct
       61 tgtataattt tggaataatt gataatcatt ttcaatagga ggaaattatg aaaaaactat
      121 tattaccatt aataattatg ttattagtgt tagctgcgtg tgggaaccaa ggtgaaaaaa
      181 ataacaaagc tgaaactaaa tcttataaaa tggacgatgg caaaacggta gatattccga
      241 aagaccctaa acgcattgca gtagttgcgc caacatatgc tggtggactt aaaaaattag
      301 gtgcaaacat tgtagctgta aatcaacaag tcgatcaaag caaagtatta aaagataaat
      361 ttaaaggtgt tacaaaaatt ggtgatggcg atgtagaaaa agttgctaaa gaaaagccag
      421 atttaattat tgtatactct actgacaaag atattaaaaa atatcaaaaa gtagcaccaa
      481 cagtagttgt tgactataat aagcataaat atttagaaca acaagaaatg ttagggaaaa
      541 ttgttggtaa agaagataaa gtaaaagctt ggaagaaaga ttgggaagaa acaactgcta
      601 aagacggtaa agaaattaaa aaagcaattg gacaagatgc aacagtgtca ttgtttgatg
      661 aatttgataa aaaattatac acttacggcg ataactgggg tcgtggtgga gaagtattat
      721 atcaagcatt tggtttgaaa atgcaaccag aacaacaaaa gttaactgca aaagcaggtt
      781 gggctgaagt gaaacaagaa gaaattgaaa aatatgctgg tgattacatt gtgagtacaa
      841 gtgaaggtaa acctacacca ggatatgaat caacaaacat gtggaagaat ttgaaagcta
      901 ctaaagaagg acatattgtt aaagttgatg ctggtacata ctggtacaac gatccttata
      961 cattagattt catgcgtaaa gatttaaaag aaaaattaat taaagctgca aaataattca
     1021 gctatataag ttagtgaaat gagagtctga aacatatcat cttttgatat tgtattaggc
     1081 tcttattttt atagctagaa agttagatat ttgtattttt ttaaataata agtgccgttg
     1141 ttatcgttca atttaattaa tgatagatta gtattattat agctaaagta gtatacctga
     1201 gaaaatagct caatgtatct ctttattgat aagttatatc ataattatat tagtgcatac
     1261 tttatggaag ggatatcagg gaatggctat caataaaaga agaggtttaa aaggattaca
     1321 acagaatgtt atgattttgt agaaagatat ataacaacgt tttataaaaa cataatattg
     1381 ttaatggaca atgaaatgta agggggattt cgagtgacta agaaagttta ttttaaccat
     1441 gatggtggtg tagatgattt agtatctcta tttttattat tacaaatgga aaacgttcaa
     1501 ttgatagggg tcagtacaat tggtgctgat tgttatttag agccatcttt gagcgcatca
     1561 gtaaaaatta ttaatcgttt ttcaaatgaa gatattcaag ttgcgccatc atatgaacga
     1621 ggaaaaaatc catttcctaa agaatggcgt atgcatgcct tttttatgga cgcattgcca
     1681 attttaaatg agccagtcaa acatgttgct tcaaatgtga gcgacaaaga agcctttgaa
     1741 gacattattc aaactttaaa gagacaatca gaaaaagtaa cattattatt tacaggcccg
     1801 cttacagatt tagcaaaagc actacaaaaa gattcatcta tcgttcagta tatagaaaaa
     1861 ttagtttgga tgggtggcac ctttttacca aaaggaaatg ttgaagaacc tgagcatgat
     1921 ggttctgcag aatggaatgc atattgggat ccagaagcgg ttaaaattgt ttttgatagc
     1981 gatatagaga ttgatatggt tgctttagaa agtacgaatc aagtgccgct aacgttagat
     2041 gttagacaaa gatgggcgaa tgaacgccaa tatacgggca ttgatttttt aggagtaagt
     2101 tatgcagcgg taccaccatt aacacacttt ataacaaatt ctacttactt tttatgggat
     2161 gttttaacga ctgcttatat tggtaacaag gacttggttc attcaattga gaaaaaagtc
     2221 gatgtaataa gttatggacc aagtcaaggt aagacatttg agtgtaaaga tgggcgcaaa
     2281 attaatgtca taaatcatgt agataacaac gcattttttg attatataac tgcacttgct
     2341 aaaaaagtaa attaacagct gtgtagaata attaaggttt taatttatat agaacaactt
     2401 attgtaaact tttcatttct taaagtttac aatggtgcta taataatggt catgaaatac
     2461 gaaaggaagt aaaaaatgac aacaaaacag ttagtatata cagctttaat gacagcgatt
     2521 atcgctattt taggaatggt accggtaatt ccactaccat tttcttcagt accaattgta
     2581 cttcaaaaca ttggtatttt cttagcaggt gtgattttag gacgtaaata tggcacatta
     2641 agtgttatcg tctttttatt attagtagtt gctggcttgc cattgttatc aggtggtcgc
     2701 ggtggcatcg gtgtattcgc aggtccttca gcagggtttt tactattata tccagttgta
     2761 gcattcatga ttggtgcgat tcgtgataga ttcatcaatg aaattaattt ctggatttta
     2821 ttcgttggta ttttagtttt tggtgttata gcattagatg ttattggtac attgattatg
     2881 ggcatgatta ttaacatacc atttacgaaa gctatttcaa tttcattagc ttatttgcct
     2941 ggtgatatat taaaagcaat tgtagcaagt ttgattggta cagctttact taatcactcg
     3001 cagtttcgtc aaattatggg attaaaataa tcatatttaa gttaagataa taaagtaatt
     3061 gaataagttg ctttgaaatt tataaaagtg aaaggagtag gtgtcaatgg ctagtataag
     3121 tatgtcagat atatattgta acggcactat atttgaaaat gacgacgagc agttaattta
     3181 tttaacgcct tcttttccac aacgatacac aagcaacaca tggatatata aaaagacgcc
     3241 tacccaagag cgatggctga aagacttaga acgtcaacat caattacata caaatcaagg
     3301 ttcaaatcat tatgcgttta gtttcccgga aaatgaacaa cttgataatc attggatggc
     3361 tatgtttaaa gatatgaatt ttgaactagg tattatggaa ttgtatgcca tagaaagtga
     3421 tgcgcttgct aatttgccgc gtaactctga cgttgaaatt gccgtcgttg acgagtcgca
     3481 tatagatgcc tatttaaaag ttgcatatca gtttagtctg ccatttggaa aagactatgc
     3541 agatgcacat gaagaaatgg taagggaaca ttatcaaaaa gatgtgatta aacgcttagt
     3601 agcttattta aataatgaac ctattggcgt tgtagatgtc attgaaagtg aaaattacat
     3661 tgaattagat ggatttggtg tattagaaca atttcgacac caaggaattg gatctacaat
     3721 tcaatcgttg ataggtgaat acgccatatc aaaaaatcac aaaccaatca tattagttac
     3781 agatggtgaa gatacagcaa aagatatgta tgcaaagcaa ggttatgtct atcaatcgtt
     3841 ttgttatcaa atattaaaag aagatattgg aaattaacga ttgatgatag gtttgtatta
     3901 gaataaaaaa gcagtaagct ggtttcgagt agaaatcaac ttactgcttt ttaaattgtt
     3961 ttgagctact tatacttata aaaatagtgc gtttaaattg ttgattcatg tagaatatcg
     4021 ttcattatga cacactataa tgaatatgtt attgttcaga gtcgatgata cgttctggat
     4081 gactatatat attaaagcca ccatttcgaa taaaacctac tgccgtaata tttaggtcat
     4141 tagctaaggt tacagcaagc gttgtcggag ctgatttaga taaaatgacg ccaacaccga
     4201 ttttcgcggc tttaattaaa atttctgatg aaatacgtcc actaaaaatt aatactttat
     4261 ctcggacagt aatatgtcgc tggatacaaa atccatataa tttatctaga gcgttatgtc
     4321 taccaatgtc ttgtcgatgt acaaaaaatg tcaaaccatc gcttatagca gcattatgta
     4381 agccacctgt ttcttggtaa atatgacttg cactttgtaa tcgagtcatc atgttaataa
     4441 tttgtattgg agttaaagtg attttagaca tagaagtttt agcgatagca gcatcatttt
     4501 gaaaataaaa ctcacgactc tttccacaac aagatgcaat cattcgtttt gtggaatatt
     4561 gaaagcgatc gcctaaatct ttattaagtt caacatgggc aaaaccttta ctatcatcaa
     4621 tcagcacaga ttttaattca tctcgcttta aaatggcacc ttccgaagcc agaaatccaa
     4681 tgactaactc ctcaaggttt gttggactgc atataacagt cgcaaattct tcaccattca
     4741 ccataattgt aagtggaaat tctgtcacat attgatctgt tgtattgaat aattttccat
     4801 cttcatatct aacaattggt tgacctaaag atacatcttt gttcattatc taaccccttt
     4861 aattagctta aactttattt taaagcaatt tgcttaaaat tttaacatat ttgcttaagt
     4921 ttgaaatttg atttataaaa ttaatagcga gcaatctgtt tgatttaaat tgaattcgag
     4981 agtatacata ctaggggatc aattaataaa tatcaatctt atgcaaattt gacaattgtt
     5041 tgaatcaata tataaacagg caacggttct tttcaaatat aatagtaagt gtataatgaa
     5101 aatgtaaata ttattaaaaa tgggggttca ctcaatgaaa atgaaacgtt ttatagctat
     5161 tgtaatggca ttatttttag tattagctgg ttgctctaat tctaacgata ataatgaaag
     5221 taaaaaagat gacgcagaca atggtaagaa acaagagatt caagttgcag cggcagcaag
     5281 tttaacagat gtaaccaaga aattagcttc agaatttaaa aaagagcata aaaatgctga
     5341 tattaaattt aactatggtg gatcaggggc attaagaaaa caaattgaat caggcgcacc
     5401 tgttgacgta tttatgtctg caaatactaa agatgtagat gcattaaaag acaagaataa
     5461 agcgcatgat acatataaat atgcgaaaaa tagtctagta ttaattggtg ataaagattc
     5521 aaattacact tcagtaaaag acttaaaaga caatgataaa ttagcattag gtgaagtgaa
     5581 aactgtacca gcaggaaaat atgcgaaaca gtatttagat aacaataact tatttaaaga
     5641 agtcgaaagt aaaatcgttt atgctaaaga tgtaaaacaa gtattaaatt atgttgaaaa
     5701 gggtaatgcg aaacaaggtt ttgtgtataa aactgactta tataaacaaa acaaaaaaat
     5761 tgatactgta aaagtaatta aagaagtaga acttaagaaa ccaatcacat acgaagctgg
     5821 tgctacatca gatagtaaat tagcaaaaga gtggatggaa ttcttaaaat cagataaagc
     5881 taaagaaata ttaaaagaat accactttgc agcataagga gttgtaatcc atgcctgact
     5941 taacaccttt ttggatatca atacgagttg ctgtaatcag tacgattatt gtaacggttt
     6001 taggtatttt tatatctaaa tggttgtatc gtcgtaaggg ttcgtgggtt aaagtattgg
     6061 aaagtttatt gatattacct attgttttgc cgccaacggt attaggtttt attctattaa
     6121 tcatcttctc gccaagagga ccaatcggtc aattctttgc gaatgtacta catttacctg
     6181 tagtgttcac tttgacaggt gctgtgatag catctgtcat tgttagtttt ccactaatgt
     6241 atcaacatac tgtgcaaggc ttcagaggta tagacacgaa aatgattaat acagctagaa
     6301 cgatgggagc aagtgaaacg aaaattttcc tcaaattaat tttaccatta gctaaacgct
     6361 ctattttagc aggtataatg atgagttttg ctcgtgcatt aggtgagttt ggtgctacat
     6421 taatggttgc aggatatatt ccaaataaaa cgaatacact acctttagaa atatacttct
     6481 tagtggaaca aggtagagaa aatgaagcgt ggttatgggt attagtgcta gtcgcattct
     6541 ctattgtggt tatatctaca attaatttat tgaataaaga taaatataag gaggtcgact
     6601 agatgcttaa aatcaatgtg aaatatcaat taaagaacac tttaattcgc atcaatatag
     6661 atgatactga accaaaaatt tatgcagttc gtggtccatc tggcattggt aaaactactg
     6721 ttttaaatat gattgccgga ttacgtaaag cagatgaagc tattatcgaa gtgaatgggc
     6781 aattgcttac tgatacgaca aaaaacgtga atgttaaaat tcaacaacga cgtattggat
     6841 atctgtttca agactaccaa ttgtttccta atatgacggt ttataaaaat attactttta
     6901 tggctgaacc atctaaacac atcgatcaat taattcaaac tttaaacatt aatcatttga
     6961 tgaaacaata tcctatgaca ttgtcaggtg gagaggcaca acgtgtagca cttgcacgtg
     7021 cacttagcac gaaaccagat ttaattttat tagatgaacc tttttctagt ttggatgata
     7081 ctacaaaaga tgagagtatt acattagtta aacgtatttt caacgaatgg caaataccaa
     7141 tcatatttgt gacacattca aactatgaag cagaacaaat ggctcatgaa attattacaa
     7201 ttgggtaatc atttatttgc cattaaagag tttagaacgt atttaaaatt gtagaagtga
     7261 atgcttctat cagcatttta atgatgtttt aaactctttt ttaggggcag tttttttgag
     7321 agacattgac gcgcgtcata taatgaaagt aatgataaaa agaaaggata acttaatgtg
     7381 agtcaagaac gttattcaag gcaaatttta tttaaacaaa taggtgaaat aggtcaaagc
     7441 aaaataaatc aaaaatgtgc gttgattatt ggtatgggcg cattaggtac acatgtggcc
     7501 gaaggacttg ttagagcagg cattgctaaa ctaatcattg ttgatagaga ttatattgaa
     7561 tttagtaatt tacaaagaca aacattgttt actgaagaag atgctttgaa aatgatgcct
     7621 aaagtggttg cagctaaaaa gcatttgcta gcgttacgta gtgatgttga tattgatggt
     7681 tgtattgccc atgtggatta ttattttttg gaaacacatg gacaggacgt tgacgttatt
     7741 attgatgcaa ccgataactt tgaaacacga caactgatta atgattttgc atataaacat
     7801 cgtatacctt ggatttatgg tggtgttgta cagagtacat atacagaagc tgcatttata
     7861 cctggtaaaa caccttgctt taactgtttg gtaccacaat tgccagcatt aaatttaaca
     7921 tgtgatacag taggggtcat tcaacctgcc gtgacgatgg caacaagttt acaattaaga
     7981 gatgcgatga aagtattaac ggaacaacca attgacacaa aaataactta tggcgatatt
     8041 tgggaaggta gtcattattc atttggtttc agtaaaatgc aacgttcaga ctgtacaact
     8101 tgtggagatg taccaagtta tccgtattta aacaagaatg aacaacgtta tgcaacattg
     8161 tgtggtagag acactgtaca gtatgaaaat gcatcaatta cacacgacat tcttgttcaa
     8221 tttttaaaac aacatcaact aaattatcgc agtaattcgt atatggttat gtttgaattt
     8281 aaaggacacc gcattgttgc ttttaaaggt ggaaggtttt taatacatgg catgacacgc
     8341 acatcagatg ccacacatct aatgaattta ttgtttggat aaaaaaagat aagacaaaag
     8401 gagtgtaata ttatgggcga acatcaaaac gttaaattga atcgtacagt taaagcagcc
     8461 gtactaacgg tatcagatac tagagacttt gttacagata aaggtggtca atgcgtgcgc
     8521 caactattac aagcagatga cgttgaagtg agtgacgcac attatacaat tgtgaaagat
     8581 gaaaaagtag ccatcacgac gcaggtgaag aagtggttag aagaagatat tgatgtcatc
     8641 attacgactg gtggaacagg tattgcacaa cgtgatgtga cgattgaagc agtaaaacca
     8701 cttttaacta aagagataga aggctttggg gaattgttta gatatttgag ttatgttgaa
     8761 gatgttggca cgcgtgcatt attgtctcgt gctgtagcag gtacagttaa taataaattg
     8821 atattttcga ttccaggatc aacaggcgca gttaaattag cattagaaaa gctcattaaa
     8881 ccagaattaa atcatctgat tcatgagctt acaaaataat ttattgacct gattggcgtt
     8941 gaaaatctcc agatttaccg ccagacttgc tttcaaggta ggtttcgcca ataatcatac
     9001 ctttatcaac tgctttcgtc atgtcgtaaa tggttaaagc cgttgctgat gcagcggtta
     9061 aagcttccat ttcaacaccg gttttgccag ttgtagagac agttgtttga atgtttaaag
     9121 tataaagggg tgcatttgtt tcatcccagc tgaagtgaac atctatgcca gtcaatggta
     9181 atggatggca catcggaata attgttgatg tatttttggc agccataata ccagcgattt
     9241 gagcagtgtt caatacattg cctttggtat tggtgttatt agaaatctgc ttaaaaatag
     9301 tttcattcac agtaatgctt gaatgagcga cagcagttct ttttgtaatt tgtttgtctg
     9361 atacatcgac cattttggcg tggccttgtt gattaatatg agtaaactca gtcattttac
     9421 ccctcctagt gcatctagta tatcatgaaa aaataaaagt tttggagatg atttttaatg
     9481 gtagtagaaa aaagaaaccc aatcccagtt aaagaagcaa ttcaacgtat cgttaatcag
     9541 cagagttcaa tgccggcaat tacggtagca cttgaaaaaa gtctaaatca tatcttagca
     9601 gaagatattg tagctactta tgatatacca aggtttgata aatcacctta tgatggtttt
     9661 gcaattcgca gtgttgattc acaaggggca agtggtcaga atcgcattga gtttaaagtg
     9721 attgatcata ttggtgcagg ttcagtttct gataaattag ttggggatca cgaagcggtg
     9781 cgtattatga ctggagcaca aataccaaat ggcgcagatg ctgttgttat gtttgaacaa
     9841 acgattgaac tagaagatac atttacaatt cgtaaaccat tttcaaaaaa tgaaaatata
     9901 tctttaaaag gtgaagaaac aaagacaggc gatgttgttc taaaaaaagg acaagtaatt
     9961 aacccagggg ctatcgcggt ccttgcaaca tatggctatg cagaggttaa agttattaag
    10021 caaccgagtg tcgctgttat tgcaacagga agcgaattat tagatgttaa tgatgtatta
    10081 gaagatggaa aaattcgtaa ctctaatggc ccaatgattc gtgccttagc agaaaaatta
    10141 ggtcttgaag ttggtattta caaaacacaa aaagatgatt tagatagtgg catccaagtc
    10201 gttaaagaag ctatggaaaa acacgatatc gttattacaa cgggtggagt ttctgttgga
    10261 gactttgact atttacctga gatttataag gctgtaaagg cggaagtgtt atttaataaa
    10321 gtagcaatgc gtcctggtag cgtaacaacg gttgcatttg cagatggaaa gtatttgttt
    10381 ggattatctg gaaatccatc agcttgtttt acaggatttg aactatttgt gaaaccagct
    10441 gttaaacata tgtgtggcgc actagaagtc ttcccgcaaa taattaaagc aacattaatg
    10501 gaagatttta ccaaggcaaa cccattcaca cgatttatac gtgctaaagc aacgttaaca
    10561 agtgctggag ctactgtagt accttcagga ttcaataaat caggtgcggt tgtagcgatt
    10621 gcacatgcta actgtatggt catgttacca ggagggtcac gtggttttaa agcggggcat
    10681 acagtagata ttatattgac tgaatctgac gctgctgaag aggaacttct tttatgattt
    10741 tacaaattgt aggttacaaa aagtctggta agacaacatt gatgaggcat attgtctctt
    10801 tcttaaagac acatggttat acagttgcta ctattaaaca tcatgggcat gctaaggaag
    10861 atattcaatt acaggattca gacgtcgatc acatgaagca ttttgaagcg ggggcagatc
    10921 aaagtattgt acaaggtttt caatatcagc aaactgtaac acgtgtagat aatcaaaatc
    10981 ttactcaaat tattgagaaa tctgttacaa ttgacaccaa tatcgtatta gttgaaggct
    11041 ttaaaaatgc tgattttgaa aaagtcgtag tctatcgaga tgaagaagag ttgcaagtat
    11101 tacaacaatt gtcgaatgtt tgttatagca ttaatgtaag ggagcatgaa gattttacag
    11161 catttgagca atggttatta aataaaatta aaaatgattg tgatacacaa ttaacataga
    11221 ggattgaaat gaatgaaaca atttgaaatc gtgatagaac cgatacaaac agaacaatat
    11281 cgtgaattca ctataaatga atatcaaggt gcagtagttg tttttaccgg tcatgttcgc
    11341 gaatggacta aaggcgtcaa aacggaatat ttagaatatg aagcgtatat tccaatggct
    11401 gaaaagaaat tggcacaaat tggagatgaa ataaatgaaa aatggcctgg aacgataacg
    11461 agtattgttc atagaatagg gccattacaa atttcagata tcgctgtatt aattgcagtt
    11521 tcttcaccgc atcgtaaaga tgcctatcga gcaaatgaat atgcaattga gcgtataaaa
    11581 gaaattgttc cgatttggaa aaaagaaatt tgggaagatg gttcaaaatg gcaagggcat
    11641 caaaaaggga attatgaaga agcgaagagg gaggaataag agagatgaag gtactttact
    11701 tcgcagaaat taaagatata ttacaaaaag cacaggaaga tattgtgctt gaacaagcat
    11761 tgactgtaca acaatttgaa gatttattgt ttgaacgtta tccgcaaatc aataataaaa
    11821 agtttcaagt tgctgtaaat gaggaatttg tacaaaaatc agatttcatt caacctaatg
    11881 atactgttgc attaattcca ccggttagtg gaggttaagg gagcatgaaa gcaataattc
    11941 ttgcaggtgg tcattcagtg cgatttggta agcccaaagc ttttgcggaa gtgaacggtg
    12001 agacctttta tagtagagta attaagacat tagaatcaac aaatatgttc aatgaaatta
    12061 ttattagtac aaatgcgcaa ttggcaacgc aatttaaata tccaaatgtt gttatagatg
    12121 atgagaatca taatgataaa ggtccattag caggaattta tacaatcatg aagcaacatc
    12181 ctgaagaaga attgtttttt gtcgtttctg ttgatacacc aatgattact ggtaaagctg
    12241 taagcacgtt gtatcagttt ttagtttctc atcttattga aaatcattta gatgtcgcag
    12301 cttttaaaga agatggacgt tttattccaa caattgcatt ttatagtccg aatgcattag
    12361 gcgctataac taaagcacta cattctgata attacagttt taaaaatata tatcatgaat
    12421 tatcaacgga ttatttggat gtcagggatg tagatgcgcc ctcatattgg tacaaaaata
    12481 taaattatca gcatgatttg gacgctttaa ttcaaaaatt gtaagctgtt aggaggtcca
    12541 caaatggtag aacaaataaa agataaacta ggacgtccca tccgtgactt acggttatct
    12601 gtgacagatc ggtgtaactt taggtgtgat tattgcatgc ctaaagaggt atttggagat
    12661 gatttcgtat ttttacctaa aaatgaactg ttaacgtttg atgaaatggc tagaatcgct
    12721 aaggtatatg cagaattagg tgtaaaaaaa atacgcatta caggtggaga accattgatg
    12781 cgacgcgatt tagatgtact tatagctaaa ttaaatcaaa tcgatggtat tgaagatatt
    12841 ggtttgacta caaatggttt gttattaaaa aagcatggac aaaagttata tgatgctggg
    12901 ctacgcagaa ttaatgtcag tttggatgct attgatgata cgctatttca atcaatcaat
    12961 aatcgtaata ttaaagcgac tacgatttta gaacaaattg attacgcgac gtctattggt
    13021 ttgaatgtaa aagtaaatgt tgttatacaa aaaggtatta acgatgatca aatcatacca
    13081 atgcttgaat attttaaaga taaacatata gagattcgat ttatagaatt tatggatgtt
    13141 ggtaatgata atggatggga tttcagtaaa gttgtaacta aagatgaaat gcttacaatg
    13201 atagagcagc actttgaaat cgatcctgta gaaccaaaat attttgggga agtagcaaaa
    13261 tattatcgcc ataaggataa tggtgttcaa tttggtttga ttacaagtgt ttcacaatca
    13321 ttttgttcta catgtacacg cgcaaggctg tcatcagatg ggaagtttta cggatgttta
    13381 tttgcaactg tcgatggatt taacgttaaa gcgtttattc gttctggcgt gaccgacgaa
    13441 gaattaaaag aacaatttaa agctttatgg caaataagag atgatcgata ttcagatgag
    13501 agaactgctc aaacagttgc caatcgtcaa cgtaaaaaga taaacatgaa ttatattggt
    13561 ggttaatgtg tagggaccac tacatattaa atcattagag atgttttaat atttctgtct
    13621 tactccctaa aatacaatat tatttattaa agtaaaaacg gtcatatcta tgccagattt
    13681 aatagaaatg atcgttttta aagtttttac aagttggcgg ggccccaaca cagaagctga
    13741 cagaaagtca gcttacaata atgtgcaagt tggcggggcc ccaacataga gaatttcaaa
    13801 aagaaattct acagacaatg caagttgggg atcaacgaaa taaattttgt gaaaatgtta
    13861 tttctgtccc actcccaaaa atcactacat aattaatctt taagggctct ttaacattga
    13921 tgtcacactc caagtcattg agttgtaata tatctttttt aggtataaat gttgtcgaat
    13981 aaacaacaag ttgtccaaaa gatataaatc taaacaagat atagctagca atttaatatt
    14041 tgtaatagat aaaatgctaa gtttgatat
//
LOCUS       DAOBBH010000054        14010 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.054, whole genome shotgun sequence.
ACCESSION   DAOBBH010000054 DAOBBH010000000
VERSION     DAOBBH010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14010
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.054"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            274..1116
                     /locus_tag="P6L01_001186"
     CDS             274..1116
                     /locus_tag="P6L01_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDP3030264.1"
                     /translation="MKIGDISIHYLNGGNTKMDGGAMFGVVPKPLWSKQYNANERNQI
                     NLPTHPILIQTAQYNLIIDAGIGNGKLSEKQLRNFGVDEESHIIADLANYNLTPKDID
                     YVLMTHMHFDHAAGLTDQAGHAIFENAIHVVQQDEWHEFIAPNIRSKSTYWDKNKGDY
                     SNKLILFEKHFEPVPGIKMQHSGGHSFGHTIITIESQGDKAVHMGDIFPTTAHKNPLW
                     VTAYDDYPMQSIREKERMIPYFIQQQYWFLFYHDENYFAVKYSDDGENIDAYILRETL
                     VDNN"
     gene            complement(1241..1552)
                     /locus_tag="P6L01_001187"
     CDS             complement(1241..1552)
                     /locus_tag="P6L01_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDP3030265.1"
                     /translation="MTKLKYIIPTIIAVAIVIISTISIIQFINRKRYNPVKVLNEVKS
                     YFMNVKGSYIVYEPFVHPETDKYRLVYQGGITTIKNGQNIHYDFYADAYTGEVINIVE
                     R"
     gene            1639..2715
                     /locus_tag="P6L01_001188"
     CDS             1639..2715
                     /locus_tag="P6L01_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDP3030266.1"
                     /translation="MNINKKVTLQRIQTLTELHGAPGFEEEVKNYMTQQMAPYVDEFI
                     ENRMGGFFGVKKSKNPNAKRVMIAAHMDEIGFMITNITKNGMIQFTNLGGVANDIWQG
                     QRLVIKNRNGDKIIGVVSNIPKHFRTGSEGAPEIKDLTLDIGAQNEDEVRERGIDIGD
                     TIVPHTPFTQLSEHRYSAKAWDNRYGCVLAIEILELLKDIELDVDLYVGANVQEEVGL
                     RGAKASAEMIDPDVAFVVDCSPANDVKGNQPLSGELGKGTLIRIKDGTMILKPVFRDY
                     LLKLVEAHDIEHQYYMSPGGTDGGEIHKANIGIPTAVIGVCARYIHSTDSVFDIRDYF
                     AARSLLSEAICNLDNNQIETLQYK"
     gene            2780..3091
                     /locus_tag="P6L01_001189"
     CDS             2780..3091
                     /locus_tag="P6L01_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDP3030267.1"
                     /translation="MKQLESEQQFESLKQGATVFEFTAGWCPDCRVIEPDLPELEARY
                     PMFDFVSVDRDKFMDICIENGIMGIPSFLVYKNGELLGSYIGKERKSIEQIDAFLAQY
                     V"
     gene            3191..4048
                     /locus_tag="P6L01_001190"
     CDS             3191..4048
                     /locus_tag="P6L01_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 domain-containing protein"
                     /protein_id="HDP3030268.1"
                     /translation="MNTFQMRDKLKERLSHLDVDFKFNREEETLRIYRTDNNKGITIK
                     LNAIVAKYEDKKEKIVDEIVYYVDEAIAQMADKTLESISSSQIMPVIRATSFDKKTKQ
                     GVPFIYDEHTAETAVYYAVDLGKSYRLIDESMLEDLKLTEQQIREMSLFNVRKLSNSY
                     TTDEVKGNIFYFINSNDGYDASRILNTAFLNEIEAQCQGEMLVAVPHQDVLIIADIRN
                     KTGYDVMAHLTMEFFTKGLVPITSLSFGYKQGHLEPIFILGKNNKQKRDPNVIQRLEA
                     NRRKFNKDK"
     gene            4077..4673
                     /locus_tag="P6L01_001191"
     CDS             4077..4673
                     /locus_tag="P6L01_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDP3030269.1"
                     /translation="MNLFYNPKYVGDVAFLQIEPVEGELNYNKKGNVVEITNEGNVVG
                     YNIFEISKDITIEEKGHIKLTDELVNVFQKRISEAGFDYKLNADLSPKFVVGYVETKD
                     KHPDADKLSVLNVNVGNDTLQIVCGAPNVEAGQKVVVAKVGAVMPSGMVIKDAELRGV
                     ASSGMICSMKELNLPNAPEEKGIMVLNDSYEIGQAFFE"
     gene            4694..8518
                     /locus_tag="P6L01_001192"
     CDS             4694..8518
                     /locus_tag="P6L01_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDP3030270.1"
                     /translation="MSWFDKLFGEDNDSNDDLIHRKKKRRQESQNIDNDHDSLLPQNN
                     DIYSRPRGKFRFPMSVAYENENVEQSADTISDEKEQYHRDYRKQSHDSRSQKRHRRRR
                     NQTTEEQNYSEQRGNSKISQQSIKYKDHSHYHTNKPDTYVSAINGIEKETHKSKTHNM
                     YSNNTNHRAKDSTPDYHKESFKTSEVPSAIFGTMKPKKLENGRIPVSKPSEKVESDKQ
                     KYDKYVAKTQTSQNKQLEQEKQNDSVVKQGTASKSSDENVSSTTKSMPNYSKVDNTIK
                     IENIYASQIVEEIRRERERKVLQKRRFKKALQQKREEHKNEEQDAIQRAIDEMYAKQA
                     ERYVGDSSLNDDSDLTDNSTDASQLHTNGIENETVSNDENKQASIQNEDTNDTHVDES
                     PYNYEEVSLNQVSTTKQLSDDEVTVSNVTSQHQSALQHNVEVNDKDELKNQSRLIADS
                     EEDGATNKEEYSGSQIDDAEFYELNDTEVDEDTTSNIEDNTNRNASEMHVDAPKTQEH
                     AVTESQVNNIDKTVDNEIELAPRHKKDDQTNLNVNSLKTNDVNDGHVVEDSSMNEIEK
                     HNAEITENVQNEAAESEQNVEEKTIENVNPKKQTEKVSTLSKRPFNVVMTPSDKKRMM
                     DRKKHSKVNVPELKPVQSKQAVSESKPASQAAPSSRSDSQESNTNAYKTNNMTSNNVE
                     NNQLIGHAETENDYQNAQQYSEQKPSADSTQTEIFEESQDDNQLENEQVDQSTSSSVS
                     EVSDITEESEETTHPNNTSGQQDNDDQQKDLQPSFSNQNEDTANENRPRTNQQDVATN
                     QAVQTSKPMIRKGPNIKLPSVSLLEEPQVIESDEDWITDKKKELNDALFYFNVPAEVQ
                     DVTEGPSVTRFELSVEKGVKVSRITALQDDIKMALAAKDIRIEAPIPGTSRVGIEVPN
                     QNPTTVNLRSIIESPSFKNAESKLTVAMGYRINNEPLLMDIAKTPHALIAGATGSGKS
                     VCINSILMSLLYKNHPEELRLLLIDPKMVELAPYNGLPHLVAPVITDVKAATQSLKWA
                     VEEMERRYKLFAHYHVRNITAFNKKAPYDERMPKIVIVIDELADLMMMAPQEVEQSIA
                     RIAQKARACGIHMLVATQRPSVNVITGLIKANIPTRIAFMVSSSVDSRTILDSGGAER
                     LLGYGDMLYLGSGMNKPIRVQGTFVSDDEIDDVVDFIKQQREPDYLFEEKELLKKTQT
                     QSQDELFDDVCAFMVNEGHISTSLIQRHFQIGYNRAARIIDQLEQLGYVSSANGSKPR
                     DVYVTEADLNKE"
     gene            8542..9855
                     /locus_tag="P6L01_001193"
     CDS             8542..9855
                     /locus_tag="P6L01_001193"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDP3030271.1"
                     /translation="MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALR
                     NKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYT
                     SVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLS
                     YKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPI
                     YYYGFKDSDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISY
                     LEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHK
                     EVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEG
                     ASLINEDSINVLEQFDNAVVLFMGAGDIQKLQNAYLDKLGMKNAF"
     gene            9929..10420
                     /locus_tag="P6L01_001194"
     CDS             9929..10420
                     /locus_tag="P6L01_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDP3030272.1"
                     /translation="MDWILPIAGIIAAIAFLILCIGIVAVLNSVKKNLDYVAKTLDGV
                     EGQVQGITRETTDLLHKVNRLTEDIQGKVDRLNSVVDAVKGIGDSVQTLNSSVDRVTN
                     SITHNISQNEDKISQVVQWSNVAMEIADKWQNRHYRRGSANYKANNVATDANHSYTSR
                     VDK"
     gene            10494..11852
                     /locus_tag="P6L01_001195"
     CDS             10494..11852
                     /locus_tag="P6L01_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030273.1"
                     /translation="MTQQQNDKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGL
                     YFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAYTKERVEGFLNKSKNEQAALKA
                     QQAAIKEEASANNLSDTSQEAQEIQEAKREAQAEADKSVAVSNEESKASALKVQQAAI
                     KEEASANNLSDTSQEAQEIQEAKKEAQAETDKSAAVSNEEPKAVALKAQQAAIKEEAS
                     ANNLSDTSQEAQEVQEAKKEAQAEKDSDTSTKDASAAKVEVSKPESQAERLANAAKQK
                     QAKLTPGSKESQLTEALFAEKPVAKNDLKEIPQLVTKKNDVSETETVNIDNKDTVKQK
                     EAKFENGVITRKADEKTTNNTAVDKKSGKQSKKTTPSNKRNASKASTNKTSGQKKQHN
                     KKSSQGAKKQSSSSKLTQKNNQTSNKNSKTTNAKSSNASKTPNAKVEKANSKIEKRTF
                     ND"
     gene            12008..12091
                     /locus_tag="P6L01_001196"
     CDS             12008..12091
                     /locus_tag="P6L01_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030274.1"
                     /translation="MLFETSLDNNSYIAHFKLKMEKYSIIK"
     gene            12546..13637
                     /locus_tag="P6L01_001197"
     CDS             12546..13637
                     /locus_tag="P6L01_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HDP3030275.1"
                     /translation="MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRI
                     YDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPE
                     DTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSP
                     YDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELL
                     KEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDI
                     SAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDA
                     GQQMDLDEFQAFYDELKPLADLYNAKKLK"
BASE COUNT         5441 a         1919 c         2571 g         4079 t
ORIGIN      
        1 agaaaattgc gcctatgaac aatgctatac taaatacctt tgatttaaaa gaataaagct
       61 tgcattttac ttgtaaattt taattattaa tttaaaatta caggccgatg gttatgatgc
      121 atttacatat aatatgattg cattaatctt tataagtagt tagaagtgta ttagtgtggg
      181 gcgtttcgtt tttgttcgca tttatgaata gtgatatgat gtaataaata catgctaatt
      241 gtaataacga atacagaaag aataggtgat gttatgaaga tcggggatat atctattcat
      301 tatctaaatg gtggcaatac aaaaatggat ggcggtgcaa tgtttggtgt tgttccgaag
      361 ccgttgtggt caaagcaata caatgcaaat gaacgaaatc aaatcaattt accgacacat
      421 ccaattttga ttcaaacggc tcaatataat ttgattatag atgcgggtat tggtaatggt
      481 aaattatctg aaaagcaatt acgtaatttt ggagtagatg aagaaagtca tataattgct
      541 gatttggcaa attataattt aacgccaaag gatattgatt atgtgctaat gacacatatg
      601 cattttgatc atgctgccgg tttgactgat caagcgggac atgcaatttt tgaaaatgcg
      661 attcatgttg tgcaacaaga tgagtggcat gagtttattg cacctaatat aagaagtaaa
      721 tcaacttact gggataagaa taaaggcgat tatagtaaca agttgatttt attcgaaaaa
      781 cattttgaac cggttccggg tatcaagatg caacatagtg gaggtcatag ctttggccac
      841 acgattatta cgattgaaag tcaaggagat aaagcagttc atatgggtga tatattccca
      901 actactgcac ataaaaatcc tctatgggta acggcatatg atgattatcc tatgcaatcg
      961 attcgtgaaa aagaacgcat gataccatat tttattcagc aacaatattg gttcttgttt
     1021 tatcatgatg aaaactactt tgctgtaaaa tacagcgatg atggtgaaaa catagatgca
     1081 tatattttac gtgaaacatt agttgataat aactaaaata aagatgtatt actaaacaaa
     1141 ttttcaaaaa taaaaaaatg agccacatcc aatcttacta attagggtgt ggctcatttt
     1201 taagttttac gatccaaatc aaatatggat aaaattcgta ttaacgctct acaatgttaa
     1261 tgacttcacc agtatatgca tctgcataaa aatcataatg aatattttga ccatttttaa
     1321 tagttgtaat tccaccttga taaactaaac ggtatttatc agtttcagga tgaacgaatg
     1381 gttcataaac gatatatgag cctttgacat tcataaaata tgattttact tcattaagta
     1441 ctttaacggg attatagcgt ttacgattaa taaattggat gattgaaatg gtagaaataa
     1501 ttacaatggc tactgcaatt attgttggaa ttatatattt cagtttagtc atcatttatg
     1561 ctcccttgat ttcaaattca tatcattagt ttaccatatt gaagatgata taataactta
     1621 taaggagtga gaacttttat gaacataaat aaaaaagtaa cattacaacg aattcaaact
     1681 ttaactgagc ttcatggggc accaggtttt gaagaagaag taaaaaatta tatgactcag
     1741 caaatggcgc cgtatgtaga tgaatttatt gaaaatcgta tgggtggatt ttttggtgtg
     1801 aaaaaatcta aaaatccaaa tgcaaaacgt gtaatgattg cagcacatat ggatgaaatc
     1861 ggatttatga ttacaaatat cactaaaaat ggaatgattc aattcacaaa tttaggtggt
     1921 gttgcaaatg atatttggca aggacaacgc ttagtaatta aaaatagaaa tggcgataaa
     1981 attatcggtg ttgtttctaa tatacctaaa cattttcgta ctggtagtga aggtgcaccg
     2041 gaaattaaag atttaacatt agatataggt gctcaaaatg aagatgaggt gcgtgagcgc
     2101 ggaatagata taggagatac aattgtacct cacacgccat tcacacagtt atctgaacat
     2161 cgatatagtg ctaaagcatg ggataatcgt tatggttgtg tcttggcaat tgaaatacta
     2221 gaattattaa aagatataga attagatgta gacttgtatg ttggcgcaaa tgttcaagaa
     2281 gaggttggat tacgaggtgc gaaagcatct gcagagatga tagacccaga cgttgcattt
     2341 gtagtcgatt gttcacctgc caatgacgtt aaaggaaacc aaccattatc tggtgaactt
     2401 ggtaaaggga cgttaattcg cataaaagac ggtacaatga ttttaaagcc tgtatttaga
     2461 gattatttat taaagttagt agaagcacat gacattgaac atcaatacta tatgtcacca
     2521 ggtggaacag atggtggaga aattcataaa gctaatattg gtattccgac tgcagttatt
     2581 ggtgtatgtg cacgatatat tcatagtaca gactcagtat ttgatataag agactatttt
     2641 gcagctagat ctttactttc agaagccatt tgtaatttag ataataatca aatagaaaca
     2701 ttacaatata aataatcggg taataacaac tattatctct aaatagttat atataatcat
     2761 taattaagga gacataaaaa tgaaacaact tgaatcagaa caacaatttg aatctttaaa
     2821 acaaggtgct acagtatttg aattcactgc aggctggtgt ccagattgta gagtgataga
     2881 accagattta ccggaattag aagcgagata tcctatgttt gacttcgtat cagtagaccg
     2941 tgacaaattt atggatattt gtattgaaaa tggtattatg gggattccaa gttttctagt
     3001 atataaaaat ggtgaactgc ttggaagtta tattggaaaa gaacgaaaat caattgaaca
     3061 gatagatgca tttttagctc aatatgtgta atttagacta gagaaaaacg gggtaatact
     3121 cgtttttctc tgttactatg tgttgattta ttgtaaacta ttataaggtg cgaaattagg
     3181 agtgttacat atgaatacct ttcaaatgag agataaatta aaggaacgtt taagccattt
     3241 agacgttgat tttaaattta atcgtgaaga agaaactttg cgtatttatc gaacagataa
     3301 taacaaaggt atcacgatta aacttaacgc tatagtcgca aaatatgaag ataaaaaaga
     3361 aaaaattgta gatgaaattg tttattacgt tgatgaagct attgcacaaa tggcagataa
     3421 aacccttgag agtatatcat ctagtcaaat tatgcctgtc attagagcga ctagctttga
     3481 taaaaaaact aaacaaggtg ttccttttat ctatgatgag catactgcag aaacagcagt
     3541 ttattatgca gtcgatttag ggaaatcata tcgtctaatt gacgaaagca tgttagagga
     3601 tttgaagtta actgaacaac aaataagaga aatgtctctg tttaatgtta gaaaattgtc
     3661 aaattcatat acgactgatg aagtaaaagg taatattttt tattttatta actcaaatga
     3721 cgggtatgat gcaagtagga tactaaatac tgcattttta aatgaaattg aggcacaatg
     3781 tcaaggcgaa atgctcgtag cagtgccaca ccaagatgtg ttaattattg cagatatacg
     3841 caataaaaca ggatatgatg tgatggcaca tttaacaatg gaatttttca ctaaaggtct
     3901 agttccaatt acatcattat cctttggata taaacagggt catcttgaac cgatatttat
     3961 tttaggtaaa aataataaac aaaaaagaga tccaaacgtg attcagcgtt tagaagcaaa
     4021 tcgtcgtaaa tttaataaag ataaatagaa ataattggat aaggagtttt gtcataatga
     4081 atttatttta caatcctaaa tatgtaggag atgtcgcatt tttacaaatt gaaccagttg
     4141 aaggtgaatt aaactacaat aaaaaaggta atgttgttga aattactaat gaaggtaatg
     4201 ttgtaggtta taatattttt gaaatttcaa aagatataac aattgaagaa aaaggtcata
     4261 ttaaattaac tgatgaactt gtaaatgtat tccaaaagcg tatttcagaa gctggttttg
     4321 attataaatt aaatgctgat ctatcaccga aatttgtagt tggctacgtt gaaactaaag
     4381 acaaacatcc tgatgcagat aaattaagtg tactaaatgt aaacgttgga aatgacacat
     4441 tacaaattgt atgtggcgcg cctaacgttg aagctggaca gaaagttgtt gttgctaaag
     4501 taggtgcagt gatgcctagc ggtatggtaa ttaaagatgc tgaattacgt ggtgttgcct
     4561 caagcggtat gatttgttca atgaaagaat tgaatttacc taatgcacct gaagaaaaag
     4621 gtattatggt attaaatgac agctatgaaa ttggacaagc attttttgaa taattaagga
     4681 aggtagtgaa aatatgagct ggtttgataa attattcggc gaagataatg attcaaatga
     4741 tgacttgatt catagaaaga aaaaaagacg tcaagaatca caaaatatag ataacgatca
     4801 tgactcatta ctgcctcaaa ataatgatat ttatagtcgt ccgaggggaa aattccgttt
     4861 tcctatgagc gtagcttatg aaaatgaaaa tgttgaacaa tctgcagata ctatttcaga
     4921 tgaaaaagaa caataccatc gagactatcg caaacaaagc cacgattctc gttcacaaaa
     4981 acgacatcgc cgtagaagaa atcaaacaac tgaagaacaa aattatagtg aacaacgtgg
     5041 gaattctaaa atatcacagc aaagtataaa atataaagat cattcacatt accatacgaa
     5101 taagccagat acatatgttt ctgcaattaa tggtattgag aaggaaacgc acaagtcaaa
     5161 aacacataat atgtattcta ataatacaaa tcatcgtgct aaagattcaa ctccagatta
     5221 tcacaaagaa agtttcaaga cttcagaggt accgtcagct atttttggca caatgaaacc
     5281 taaaaagtta gaaaatggtc gtatccctgt aagtaaacct tcagaaaaag ttgagtcaga
     5341 taaacaaaaa tatgataaat atgtagctaa gacgcaaacg tctcaaaata aacaattaga
     5401 acaagaaaaa caaaatgata gtgttgtcaa acaaggaact gcatctaaat catctgatga
     5461 aaatgtatca tcaacaacaa aatcaatgcc taattattca aaagttgata atactatcaa
     5521 aattgaaaat atttatgctt cacaaattgt tgaagaaatt agacgtgaac gagaacgtaa
     5581 agtgcttcaa aagcgtcgat ttaaaaaagc gttgcaacaa aagcgtgaag aacataaaaa
     5641 cgaagagcaa gatgcaatac aacgtgcaat tgatgaaatg tatgctaaac aagcggaacg
     5701 ctatgttggt gatagttcat taaatgatga tagtgactta acagataata gtacagatgc
     5761 tagtcagctt catacaaatg gcatagagaa tgaaactgta tcaaatgatg aaaataaaca
     5821 agcgtcaata caaaatgaag acactaatga cactcatgta gatgaaagtc catacaatta
     5881 tgaggaagtt agtttgaatc aagtatcgac aacaaaacaa ttgtcagatg atgaagttac
     5941 ggtttcgaat gtaacgtctc aacatcaatc agcactacaa cataacgttg aagtaaatga
     6001 taaagatgaa ctaaaaaatc aatccagatt aattgctgat tcagaagaag atggagcaac
     6061 gaataaagaa gaatattcag gaagtcaaat cgatgatgca gaattttatg aattaaatga
     6121 tacagaagta gatgaggata ctacttcaaa tatcgaagat aataccaata gaaacgcgtc
     6181 tgaaatgcat gtagacgctc ctaaaacgca agagcacgca gtaactgaat ctcaagttaa
     6241 taatatcgat aaaacggttg ataatgaaat tgaattagca ccgcgtcata aaaaagatga
     6301 ccaaacaaac ttaaatgtca actcattgaa aacgaatgat gtgaatgatg gtcatgttgt
     6361 ggaagattca agcatgaatg aaatagaaaa gcataacgca gaaattacag aaaatgtgca
     6421 aaacgaagca gctgaaagtg aacaaaatgt cgaagagaaa actattgaaa acgtaaatcc
     6481 aaagaaacag actgaaaagg tttcaacttt aagtaaaaga ccatttaatg ttgtcatgac
     6541 gccatctgat aaaaagcgta tgatggatcg taaaaagcat tcaaaagtca atgtgcctga
     6601 attaaagcct gtacaaagta aacaagctgt gagtgaaagc aagcctgcga gtcaagccgc
     6661 accatcatca agatctgatt cacaagagtc aaacacaaat gcatataaaa caaataatat
     6721 gacatcaaac aatgttgaga acaatcaact tattggtcat gcagaaacag aaaatgatta
     6781 tcaaaatgca caacaatatt cagagcagaa accttctgct gattcaactc aaacggaaat
     6841 atttgaagaa agccaagatg ataatcaatt ggaaaatgag caagttgatc aatcaacttc
     6901 gtcttcagtt tcagaagtaa gcgacataac tgaagaaagc gaagaaacaa cacatccaaa
     6961 caatactagt ggacaacaag ataatgatga tcaacaaaaa gatttacagc cttcattttc
     7021 aaatcaaaat gaagatacag ctaatgaaaa tagacctcgg acgaaccaac aagatgttgc
     7081 aacaaatcaa gctgtacaaa catctaagcc gatgattcgt aaaggcccaa atattaaatt
     7141 gccaagtgtt tcattactag aagaaccaca agttattgag tcggacgagg actggattac
     7201 agataaaaag aaagaactga atgacgcatt attttacttt aatgtacctg cagaagtaca
     7261 agatgtaact gaaggtccaa gtgttacaag atttgaatta tcagttgaaa aaggtgttaa
     7321 agtttcaaga attacggcat tacaagatga cattaaaatg gcattggcag cgaaagatat
     7381 tcgtatagaa gcgcctattc caggaactag tcgtgttggt attgaagttc cgaaccaaaa
     7441 tccaacgaca gtcaacttac gttctattat tgaatctcca agttttaaaa atgctgaatc
     7501 taaattaaca gttgcgatgg ggtatagaat taataatgaa ccattactta tggatattgc
     7561 taaaacgcca cacgcactaa ttgcaggtgc aactggatca gggaaatcag tttgtatcaa
     7621 tagtattttg atgtctttac tatataaaaa tcaccctgag gaattaagat tattacttat
     7681 cgatccaaaa atggttgaat tagctcctta taatggtttg ccacatttag ttgcaccggt
     7741 aattacagat gtcaaagcag ctacacagag tttaaaatgg gccgtagaag aaatggaacg
     7801 acgttataag ttatttgcac attaccatgt acgtaatata acagcattta acaaaaaagc
     7861 accatatgat gaaagaatgc caaaaattgt cattgtaatt gatgagttgg ctgatttaat
     7921 gatgatggct ccgcaagaag ttgagcagtc tattgctaga attgctcaaa aagcaagagc
     7981 atgtggtatt catatgttag tagctacgca aagaccatct gtcaatgtaa ttacaggttt
     8041 aattaaagcc aacataccaa caagaattgc atttatggta tcatcaagtg tagattcgag
     8101 aacgatatta gacagtggtg gagcagaacg cttgttagga tatggcgata tgttatatct
     8161 tggtagcggt atgaataaac cgattagagt tcaaggtaca tttgtttctg atgacgaaat
     8221 tgatgatgtt gttgatttta tcaaacaaca aagagaaccg gactatctat ttgaagaaaa
     8281 agaattgttg aaaaaaacac aaacacaatc acaagatgaa ttatttgatg atgtttgtgc
     8341 atttatggtt aatgaaggac atatttcaac atcattaatc caaagacatt tccaaattgg
     8401 ctataataga gcagcaagaa ttatcgatca attagagcaa ctcggttatg tttcgagtgc
     8461 taatggttca aaaccaaggg atgtttatgt tacggaagca gatttaaata aagaataatt
     8521 atgagtaagg agttttatat aatgacacac tatcattttg tcggaattaa aggttctggc
     8581 atgagttcat tagcacaaat catgcatgat ttaggacatg aagttcaagg atcggatatt
     8641 gagaactacg tatttacaga agttgctctt agaaataagg ggataaaaat attaccattt
     8701 gatgctaata acataaaaga agatatggta gttatacaag gtaatgcatt cgcgagtagc
     8761 catgaagaaa tagtacgtgc acatcaattg aaattagatg ttgtaagtta taatgatttt
     8821 ttaggacaga ttattgatca atatacttca gtagctgtaa ctggtgcaca tggtaaaact
     8881 tctacaacag gtttattatc acatgttatg aatggtgata aaaagacttc atttttaatt
     8941 ggtgatggca caggtatggg attgcctgaa agtgattatt tcgcttttga ggcatgtgaa
     9001 tatagacgtc actttttaag ttataaacct gattacgcaa ttatgacaaa tattgatttc
     9061 gatcatcctg attattttaa agatattaat gatgtttttg atgcattcca agaaatggca
     9121 cataatgtta aaaaaggtat tattgcttgg ggtgatgatg aacatctacg taaaattgaa
     9181 gcagatgttc caatttatta ttatggattt aaagattcgg atgacattta tgctcaaaat
     9241 attcaaatta cggataaagg tactgctttt gatgtgtatg tggatggtga gttttatgat
     9301 cacttcctgt ctccacaata tggtgaccat acagttttaa atgcattagc tgtaattgcg
     9361 attagttatt tagagaagct agatgttaca aatattaaag aagcattaga aacgtttggt
     9421 ggtgttaaac gtcgtttcaa tgaaactaca attgcaaatc aagttattgt agatgattat
     9481 gcacaccatc caagagaaat tagtgctaca attgaaacag cacgaaagaa atatccacat
     9541 aaagaagttg ttgcagtatt tcaaccacac actttctcta gaacacaagc atttttaaat
     9601 gaatttgcag aaagtttaag taaagcagat cgtgtattct tatgtgaaat ttttggatca
     9661 attagagaaa atactggcgc attaacgata caagatttaa ttgataaaat tgaaggtgca
     9721 tcgttaatta atgaagattc tattaatgta ttagaacaat ttgataatgc tgttgtttta
     9781 tttatgggtg caggtgatat tcaaaaatta caaaatgcat atttagataa attaggcatg
     9841 aaaaatgcgt tttaatatgt ttataataga gtagtatggg tatttattat taatgacatt
     9901 attacatgtt aattaggagg cgtttttaat ggattggatt ttaccaattg ctggaattat
     9961 cgctgcgatt gcattcttaa ttttatgtat cggtatcgta gctgtattaa attctgttaa
    10021 gaaaaactta gattatgttg caaaaacact tgacggtgta gaaggtcaag ttcaaggtat
    10081 tactcgtgaa acaacagatt tacttcataa agtaaaccgt ttaactgagg atatccaagg
    10141 taaagtagat cgtttaaact cagttgtaga tgctgttaaa ggtatcggtg actcagtaca
    10201 aacgttaaac agctctgtag atcgtgtaac aaattcaatt acacataata tttctcaaaa
    10261 tgaagataaa atctcacaag ttgttcaatg gtcaaatgtt gcaatggaaa ttgcagacaa
    10321 atggcaaaat agacactacc gtcgtggaag tgcaaattac aaagctaata atgtagcaac
    10381 tgatgcaaat catagctata cttctagagt agataaataa ttaataatta aacaaatgca
    10441 cttaaatgag gttgttactt atttaagtgc atttgttata ggaggatata caaatgacgc
    10501 agcaacaaaa tgataaaaga acattaaaaa ataaacacac ttatcaaaat gaaccactac
    10561 caaaccgtaa agattttgtt gttagtttta taactggcgc gcttgttggt tcagctttag
    10621 gtttgtattt taaaaataaa gtttatcaaa aagcagatga tttaaaagtc aaagaacaag
    10681 aactgtcgca aaagtttgaa gaaagaaaaa cgcaacttga agaaacggtt gcctatacaa
    10741 aagaacgtgt tgaaggattt ttaaacaaat ctaaaaatga gcaagcggca ttaaaggcac
    10801 aacaagcagc gataaaagaa gaagcaagtg caaataattt aagtgataca tcacaagaag
    10861 cacaagagat tcaagaagct aaaagagaag cacaagcaga agcggataaa agtgtggctg
    10921 tatcaaatga agagtcaaaa gcatcggcat taaaagtaca acaagcagca ataaaagaag
    10981 aagcaagtgc aaataattta agtgacacgt cacaagaggc acaagagatt caagaagcta
    11041 aaaaagaagc acaagcagaa acagataaaa gtgcagctgt atcaaatgaa gaaccaaaag
    11101 cagtggcatt gaaagcacaa caagcagcga taaaagaaga agcaagtgca aataatttaa
    11161 gtgatacatc acaagaggca caagaggttc aagaagctaa aaaagaagca caagcagaga
    11221 aagacagtga cacatcaact aaagatgcaa gtgcagcaaa ggtagaagta tcaaaaccag
    11281 agtcacaagc tgaaagatta gcaaacgctg caaaacagaa gcaagctaaa ttaacaccag
    11341 gttcaaaaga gagtcaatta actgaagcgt tatttgcaga aaaaccagtt gctaaaaatg
    11401 acttgaaaga aattcctcaa ttagttacta aaaagaatga tgtatcagag acagagacgg
    11461 ttaatataga taataaagac actgttaaac aaaaagaagc taaatttgaa aatggtgtta
    11521 ttacacgtaa agctgatgaa aaaacaacta ataatacagc tgttgacaag aaatcaggta
    11581 aacaatctaa aaaaacaaca ccttcaaata aacgaaatgc atcaaaagca tctacaaata
    11641 aaacttcagg tcagaaaaag caacataata agaaatcatc acaaggtgca aagaaacaaa
    11701 gtagttcaag taagttaact caaaagaata atcaaactag taataagaat tcaaaaacaa
    11761 caaatgctaa atcatccaat gcatcaaaaa cgccaaatgc taaagttgaa aaagctaata
    11821 gtaaaataga gaaacgtaca ttcaatgact aagattacta aaatcgttga ataatcaatc
    11881 atagcagtaa gctatgtatt atatttttaa aaattgaatt tcaaaataaa aggtaggcat
    11941 tcaggtgctt accttttttg atatcatcag tttattttga aaggcaacgc gatcatttca
    12001 tgtatttatg ctgtttgaaa catcgttaga taacaatagt tatattgcac attttaagct
    12061 gaagatggaa aaatattcga taatcaaata aacaatgaat cttagaaggt acaatgacgt
    12121 tcactaattc aaatatagcc gctcatgttg gtgagtgatg ttcactatag atttatatta
    12181 atatacaaag gttgttaatt tttattaagc gttaggttga atgtatgaga attttagatt
    12241 tataatagaa gatagaaacg aaaatttttc ttaaaagcag taatgttgac tcaaaataag
    12301 ctataataat gacacttatt taattgatta acatttgcta ataaatatca atatagaata
    12361 taactttcca ataatgactg agaaaatcga aatgtcagtc tcgaatcata taattagaaa
    12421 attgattatt ttctgtcaat ttagggttga actatacata tgatattgtt agaatatttt
    12481 ttaacattat attttattgc tttaaagtgg aatatacttg aaataattcg tagaggtgag
    12541 taaggatgag taataaatta gaatcataca gaagtgagat tgtctcactg aatcatcaaa
    12601 ttttagattt attatctaaa cgtggtgaac tagcacaaaa aattggggag gaaaaattaa
    12661 aacaaggtac acgtatctat gatccacaac gcgaaaaaga aatgcttaac gacttaattg
    12721 atagcaacaa aggaccattt aacgataata caattaagca attatttaaa gaaattttta
    12781 aagcctctac agatttacaa aaatctgaaa atgaaaaaca cttatacgta tcacgtaagt
    12841 tgaaacctga agatacgatt gtaacatttg ataatggggg cattattgga gacggcaata
    12901 aatcatttgt atttgggcca tgttcagttg aatcatttga acaagttgaa gctgttgcta
    12961 aaaacttaca tgctaaaggt gaaaaattta ttcgtggcgg tgcatttaaa ccacgtacat
    13021 caccatatga tttccaaggc ctaggtgttg aaggacttaa aatacttaaa cagattaaag
    13081 ataaatatga tttaaatgtt gtcagcgaaa tcgtaaatcc aaatgatttt gaagtggctg
    13141 atgagtattt agacgtattc caaattggtg cacgtaatat gcaaaacttc gagttattaa
    13201 aagaagctgg ccgtacgaaa aagcctattc tattaaaacg tggtttatct gctacaatcg
    13261 aagagtttgt ttatgcagct gaatacattg cttcacaagg taatcaaaac attattttat
    13321 gtgaacgtgg aatccgaact tatgaaaagg cgacacgtaa cactttagat atttcagcag
    13381 taccaatttt aaaacaaggt acacacttac cagtcatggt agatgttacg catagtacag
    13441 gtcgtaaaga tatcatgtta ccaactgcga aagcagcatt agcagttggt gctgatggag
    13501 ttatggctga ggtgcatcca gatccatctg ttgcacttag tgatgcgggt caacaaatgg
    13561 atttagatga attccaagca ttttatgatg aattaaagcc tttagctgat ttatataacg
    13621 ctaaaaagtt aaaataatat tccaaggaaa ctatagacta cttaactaat atgtcatgtt
    13681 gaagtagaat attatctttg aatcgacaat tttaaactta cagccattct aagagtatat
    13741 tacttttaga gtggctatta ttttttgtat agaaataaag gtatactgca cttaacgatt
    13801 gttataatac ttcgacactt gttcaatttc acaattatta aagattatga ctgatagcag
    13861 taattaaaat tataactatg aattatctgt aaaatataat agattcacac atttgttgct
    13921 gaaatgtgaa catttttcaa caaatgcaat tgatgtttga aaaggctttc tcaaaacatt
    13981 acaattaaaa atgaaaaaag tttatataaa
//
LOCUS       DAOBBH010000055        13694 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.055, whole genome shotgun sequence.
ACCESSION   DAOBBH010000055 DAOBBH010000000
VERSION     DAOBBH010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13694
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.055"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            59..352
                     /gene="esxA"
                     /locus_tag="P6L01_001198"
     CDS             59..352
                     /gene="esxA"
                     /locus_tag="P6L01_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxA"
                     /protein_id="HDP3030276.1"
                     /translation="MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWE
                     GQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ"
     gene            435..3464
                     /gene="esaA"
                     /locus_tag="P6L01_001199"
     CDS             435..3464
                     /gene="esaA"
                     /locus_tag="P6L01_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HDP3030277.1"
                     /translation="MKKKNWIYALIVTLIIIIAIVSMIFFVQTKYGDQSEKGSQSVSN
                     KNNKIHIAIVNEDQPTTYNGKKVELGQAFIKRLANEKNYKFETVTRNVAESGLKNGGY
                     QVMIVIPENFSKLAMQLDAKTPSKISLQYKTAVGQKEEVAKNTEKVVSNVLNDFNKNL
                     VEIYLTSIIDNLHNAQKNVGAIMTREHGVNSKFSNYLLNPINDFPELFTDTLVNSISA
                     NKDITKWFQTYNKSLLSANSDTFRVNTDYNVSTLIEKQNSLFDEHNTAMDKMLQDYKS
                     QKDSVELDNYINALKQMDSQIDQQSSMQDTGKEEYKQTVKENLDKLREIIQSQESPFS
                     KGMIEDYRKQLTESLQDELANNKDLQDALNSIKMNNAQFAENLEKQLHDDIVKEPDTD
                     TTFIYNMSKQDFIAAGLNEGEANKYEAIVKEAKRYKNEYNLKKPLAEHINLTDYDNQV
                     AQDTSSLINDGVKVQRTETIKSNDINQLTVATDPHFNFEGDIKINGKKYDIKDQSVQL
                     DTSNKEYKVEVNGVAKLKKDAEKDFLKDKTMHLQLLFGQANRQDEPNDKKATSVVDVT
                     LNHNLDGRLSKDALSQQLSALSRFDAHYKMYTDTKGREDKPFDNKRLIDMMVDQVIND
                     MESFKDDKVAVLHQIDSMEENSDKLIDDILNNKKNTTKNKEDISKLIDQLENVKKTFA
                     EEPQEPKIDKGKNDEFNTMSSNLDKEISRISEKSTQLLSDTQESKTIADSVSGQLNQL
                     DNNVNKLHATGRALGVRANDLNRQMAKNDKDNELFAKEFKKVLQNSKDGDRQNQALKA
                     FMSNPVQKKNLENVLANNGNTEVISPTLFVLLMYLLSMITAYIFYSYERAKGQMNFIK
                     DDYSSKNHLWNNVITSGVIGTTGLVEGLIVGLIAMNKFHVLAGYRAKFILMVILTMMV
                     FVLINTYLLRQVKSIGMFLMIAALGLYFVAMNNLKAAGQGVTNKISPLSYIDNMFFNY
                     LNAEHPIGLALVILTVLVIIGFVLNMFIKHFKKERLI"
     gene            3464..3922
                     /gene="essA"
                     /locus_tag="P6L01_001200"
     CDS             3464..3922
                     /gene="essA"
                     /locus_tag="P6L01_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HDP3030278.1"
                     /translation="MLMNSVIALTFLTASSNNGGLNIDVQQEEEKRINNDLNQYDTTL
                     FNKDSKEVNDAIAKQKKERQQQIKNDMFQNQASHSTRLNETKKVLFSKSNLEKTSESD
                     KSPYIQNKQEKKIFPYILMSVGAFLTLGFVIFSIHKGRRTKNESARKSNI"
     gene            3894..4136
                     /gene="esaB"
                     /locus_tag="P6L01_001201"
     CDS             3894..4136
                     /gene="esaB"
                     /locus_tag="P6L01_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaB"
                     /protein_id="HDP3030279.1"
                     /translation="MNQHVKVTFDFTNYNYGTYDLAVPAYLPIKNLIALVLDSLDISI
                     FDVNTQIKVMTKGQLLVENDRLIDYQIADGDILKLL"
     gene            4149..5483
                     /gene="essB"
                     /locus_tag="P6L01_001202"
     CDS             4149..5483
                     /gene="essB"
                     /locus_tag="P6L01_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HDP3030280.1"
                     /translation="MVKNHNPKNEMQDMLTPLDAEEAAKTKLRLDMREIPKSSIKPEH
                     FHLMYLLEQHSPYFIDAELTELRDSFQIHYDINDNHTPFDNIKSFTKNEKLRYLLNIK
                     NLEEVNRTRYTFVLTPDELFFTRDGLPIAKTRGLQNVVDPLPVSEAEFLTRYKALVIC
                     AFNEKQSFDALVEGNLELHKGTPFETKVIEAATLDLLTAFLDEQYQKQEQDYSQNYAY
                     VRKVGHTVFKWVAIGMTTLSVLLIAFLAFLYFSVMKHNERIEKGYQAFVKDDYTQVLN
                     TYDDLDGKKLDKEALYIYAKSYIQTNKQGLEKDKKENLLNNVTPNSNKDYLLYWMELG
                     QGHLDEAINIATYLDDNDITKLALINKLNEIKNNGDLSNDKRSEETKKYNDKLQDILD
                     KEKQVKDEKAKSEEEKAKAKDEKLKQQEENEKKQKEQAQKDKEKRQEAERKK"
     gene            5505..9944
                     /gene="essC"
                     /locus_tag="P6L01_001203"
     CDS             5505..9944
                     /gene="essC"
                     /locus_tag="P6L01_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HDP3030281.1"
                     /translation="MHKLIIKYNKQLKMLNLRDGKTYTISEDERADITLKSLGEVIHL
                     EQNNQGTWQANHTSINKVLVRKGDLDDITLQLYTEADYASFAYPSIQDTMTIGPNAYD
                     DMVIQSLMNAIIIKDFQSIQESQYVRIVHDKNTDVYINYELQEQLTNKAYIGDHIYVE
                     GIWLEVQADGLNVLSQNTVASSLIRLTQEMPHAQADDYNTYHRSPRIIHREPTDDIKI
                     ERPPQPIQKNNTVIWRSIIPPLVMIALTVVIFLVRPIGIYILMMIGMSTVTIVFGITT
                     YFSEKKKYNKDVEKREKDYKAYLDNKSKEINKAIKAQRFSLNYHYPTVAEIKDIVETK
                     APRIYEKTSHHHDFLHYKLGIANVEKSFKLDYQEEEFNQRRDELFDDAKELYEFYTDV
                     EQAPLINDLNHGPIAYIGARHLILEELEKMLIQLSTFHSYHDLEFLFVTREDEVETLK
                     WARWLPHMTLRGQNIRGFVYNQRTRDQILTSIYSMIKERIQAVRERSRSNEQIIFTPQ
                     LVFVITDMSLIIDHVILEYVNQDLSEYGISLIFVEDVIESLPEHVDTIIDIKSRTEGE
                     LITKEKELVQLKFTPENIDNVDKEYIARRLANLIHVEHLKNAIPDSITFLEMYNVKEV
                     DQLDVVNRWRQNETYKTMAVPLGVRGKDDILSLNLHEKAHGPHGLVAGTTGSGKSEII
                     QSYILSLAINFHPHEVAFLLIDYKGGGMANLFKDLVHLVGTITNLDGDEAMRALTSIK
                     AELRKRQRLFGEHDVNHINQYHKLFKEGVATEPMPHLFIISDEFAELKSEQPDFMKEL
                     VSTARIGRSLGIHLILATQKPSGVVDDQIWSNSKFKLALKVQDRQDSNEILKTPDAAD
                     ITLPGRAYLQVGNNEIYELFQSAWSGATYDIEGDKLEVEDKTIYMINDYGQLQAINKD
                     LSGLEDEETKENQTELEAIIDHIESITTRLEIEEVKRPWLPPLPENVYQEDLVETDFR
                     KLWSDDAKEVELTLGLKDVPEEQYQGPMVLQLKKAGHIALIGSPGYGRTTFLHNIIFD
                     VARHHRPDQAHMYLFDFGTNGLMPVTDIPHVADYFTVDQEDKIAKAIRIFNDEIDRRK
                     KILSQYRVTSISEYRKLTGETIPYVFILIDNFDAVKDSPFQEVFENMMIKMTREGLAL
                     DMQVTLTASRANAMKTPMYINMKTRIAMFLYDKSEVSNVVGQQKFAVKDVVGRALLSS
                     DDNVSFHIGQPFKHDETKSYNDQINDEVSAMTEFYKGETPNDIPMMPDEIKYEDYRES
                     LSLPDIVANGALPIGLDYEGVTLQKIKLTEPAMISSENPREIAHIAEIMMKEIDILNE
                     KYAICIADSSGEFKAYRHQVANFAEEREDIKAIHQLMIEDLKQREMDGPFEKDSLYII
                     NDFKTYIDCTYIPEDDVKKLITKGPELGLNILFVGIHKELIDAYDKQIDVARKMINQF
                     SIGIRISDQQFFKFRFIQREPVIKENEAYMVANQAYQKIRWFK"
     gene            9974..10366
                     /gene="esaC"
                     /locus_tag="P6L01_001204"
     CDS             9974..10366
                     /gene="esaC"
                     /locus_tag="P6L01_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion substrate EsaC"
                     /protein_id="HDP3030282.1"
                     /translation="MNFNDIETMVKSKFKDIKKHAEEIAHEIEVRSGYLRKAEQYKRL
                     EFNLSIALDDVESTAKDVQTAKSSANKDSVSVKGKAPNTLYIEKRNLMKQKLEMLGED
                     IDKNKESLQKAKGIAGEKASEYFNKAMN"
     gene            10382..10696
                     /gene="esxB"
                     /locus_tag="P6L01_001205"
     CDS             10382..10696
                     /gene="esxB"
                     /locus_tag="P6L01_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxB"
                     /protein_id="HDP3030283.1"
                     /translation="MGGYKGIKADGGKVNQAKQLAAKIAKDIEACQKQTQQLAEYIEG
                     SDWEGQFANKVKDVLLIMAKFQEELVQPMADHQKAIDNLSQNLAKYDTLSIKQGLDRV
                     NP"
     gene            10696..11370
                     /locus_tag="P6L01_001206"
     CDS             10696..11370
                     /locus_tag="P6L01_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5081 family protein"
                     /protein_id="HDP3030284.1"
                     /translation="MKDVKRIDYFSYEELTILGGSKLPLVNFELFDPSNFEEAKVALI
                     EKELVTENDKLTDAGFKVATLVREYISAIVNIRINDMYFAPFSYEKDEYILLSRFKNN
                     GFQIRIINKDIAWWSIVQSYPLLMRQEKSNDWDFKQIDDEILENLNNESIDTIGRVLE
                     IEIYNHQGDPQQSLYNIYEQNDLLLIRYPLKDKVLNVHIGVINTFIRELFGFDTDENH
                     INKAEE"
     gene            11370..11687
                     /locus_tag="P6L01_001207"
     CDS             11370..11687
                     /locus_tag="P6L01_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030285.1"
                     /translation="MTLSGKISVKAETIAHVVKELEIISQKYDEIAQNFGKIAQLNYY
                     SSEKAAHSMENGYSSAATVISGLKGPLSTLGGGVMNSAQKFFEADEHWGTEFAKLYYN
                     IEG"
     gene            11697..13541
                     /locus_tag="P6L01_001208"
     CDS             11697..13541
                     /locus_tag="P6L01_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease YeeF family protein"
                     /protein_id="HDP3030286.1"
                     /translation="MTKDIEYLTADYDNEKSSIQSVIDAIEGQDFLDVDTTMDDAVSD
                     VSSLDEDGAISLTSSVVGPQGSKLMGYYQNELYDYASQLDSKMKEIIDTPFIEDIDKA
                     FKGITNVKLENILIKNGGGHGRDTYGASGKIAKGDAKKSDSDVYSIDEILKSDQEFVK
                     VIDQHYKEMKKEDKKLSKSDFEKMMTQGASCDYMTVAEAEEIEEQKKKEEAIEIAALA
                     GMVVLSCINPVAGAVAIGAYSAYSAANAATGKNIVTGRKLSKEERIMEGLSLIPLPGM
                     GFLKGAGKSLMKLGFKGGEKFAVKTGLQKTMQQAVSRISPKMGMMKNSVLNQSRNFAQ
                     NTHVGQMLSNMRGQATHTVQQSRNWIGQQSQNVKRIVNNGLDKEIAHPFKQQLAPAGM
                     GGIKFAETTTLRNMGQNMKRAVTPQNHVTHGPKDSMVRSEGKHSVSSHEINLSKYVES
                     PNYTKVKYGEQYARLRPKKLKANIEYTTPTGHIYRTDHKGRIKEVYVDNLSLKDGGRN
                     NHAQRTVGGEDRLPDDDGGHLIARMFGGSKDIDNLVAQSKYINRSFKENGDWYKLEKL
                     WEKAIKSGKSIENIKIEVKYKGNSQRPTIFKVEYEINGKRNIRRILNK"
     gene            13552..>13694
                     /locus_tag="P6L01_001209"
     CDS             13552..>13694
                     /locus_tag="P6L01_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HDP3030287.1"
                     /translation="MTFEEKLSKIYNEIANEISNMIPVEWEKVYTMAYIDDGGGEVFF
                     NYT"
BASE COUNT         5374 a         1893 c         2590 g         3837 t
ORIGIN      
        1 aaaaatagtg tccctaaaaa gttttgataa acttaaaata ttcaggaggt ttctagttat
       61 ggcaatgatt aagatgagtc cagaggaaat cagagcaaaa tcgcaatctt acgggcaagg
      121 ttcagaccaa atccgtcaaa ttttatctga tttaacacgt gcacaaggtg aaattgcagc
      181 gaactgggaa ggtcaagctt tcagccgttt cgaagagcaa ttccaacaac ttagtcctaa
      241 agtagaaaaa tttgcacaat tattagaaga aattaaacaa caattgaata gcactgctga
      301 tgccgttcaa gaacaagacc aacaactttc taataatttc ggtttgcaat aagcattctg
      361 aaattggcaa agtcacattt tctaatgtgg ctttgcttat cattttttaa gaaaacaact
      421 gaaaggaaat aagcatgaaa aagaaaaatt ggatttatgc attaattgtc actttaatta
      481 ttataattgc catagttagt atgatatttt ttgttcaaac aaaatatgga gatcaatcag
      541 aaaaaggatc ccaaagtgta agtaataaaa ataataaaat acatatcgca attgttaacg
      601 aggatcaacc aacgacatat aatggtaaaa aagttgagct gggtcaagca tttattaaaa
      661 ggttagcaaa tgagaaaaac tataaatttg aaacagtaac aagaaacgtt gctgagtctg
      721 gtttgaaaaa tggtggatac caagtcatga ttgttatccc agaaaacttt tcaaaattgg
      781 caatgcaatt agacgctaaa acaccatcga aaatatcgct acagtataaa acagctgtag
      841 gacaaaaaga agaagtagct aaaaacacag aaaaagttgt aagtaatgta cttaacgact
      901 ttaacaaaaa cttagtcgaa atttatttaa caagcatcat tgataattta cataatgcac
      961 aaaaaaatgt tggcgctatt atgacgcgtg aacatggtgt gaatagtaaa ttctcgaatt
     1021 acttattaaa tccaattaac gacttcccgg aattatttac agatacgctt gtaaattcaa
     1081 tttctgcaaa caaagacatt acaaaatggt tccaaacata caataaatca ttattgagtg
     1141 cgaattcaga tacgttcagg gtgaacacag attataatgt ttcgacttta attgaaaaac
     1201 aaaattcatt atttgacgag cacaatacag cgatggataa aatgttacaa gattataaat
     1261 cgcaaaaaga tagtgtggaa cttgataact atatcaatgc attaaaacag atggacagcc
     1321 aaattgatca acaatcaagt atgcaagata caggtaaaga agaatataaa caaactgtta
     1381 aagaaaactt agataaatta agagaaatca ttcaatcaca agagtcacca ttttcaaaag
     1441 gtatgattga agactaccgt aagcaattaa cagaatcact gcaagatgag cttgcaaata
     1501 acaaagactt acaagatgcg ctaaatagca ttaaaatgaa caatgctcaa ttcgctgaaa
     1561 acttagaaaa acaacttcat gatgatattg tcaaagaacc tgatacagat acaacattta
     1621 tctataacat gtctaaacaa gactttatag ctgcaggttt aaatgagggt gaagctaata
     1681 aatacgaagc aattgtcaaa gaagcaaaac gttataaaaa cgaatataat ttgaaaaaac
     1741 cgttagcaga acacattaat ttaacagatt acgataacca agttgcgcaa gacacaagta
     1801 gtttgattaa tgatggtgtc aaagtgcaac gtactgaaac gattaaaagt aatgatatta
     1861 atcaattaac tgttgcaaca gatcctcatt ttaactttga aggcgacatt aaaattaatg
     1921 gtaaaaaata tgacattaag gatcaaagtg ttcaactcga tacatctaac aaggaatata
     1981 aagttgaagt caatggcgtt gctaaattga aaaaggatgc tgagaaagat ttcttaaaag
     2041 ataaaacaat gcatttacaa ttgttatttg gacaagcaaa tcgtcaagat gaaccaaatg
     2101 ataagaaagc aacgagtgtt gtggatgtaa cattgaatca taaccttgat ggtcgcttat
     2161 cgaaagatgc attaagccag caattgagtg cattatctag gtttgatgca cattataaaa
     2221 tgtacacaga tacaaaaggc agagaagata aaccattcga taacaaacgt ttaattgata
     2281 tgatggttga ccaagttatc aatgacatgg aaagtttcaa agacgataaa gtagctgtgt
     2341 tacatcaaat tgattcaatg gaagaaaact cagacaaact gattgatgac attttaaata
     2401 acaaaaagaa tacaacaaaa aataaagaag atatttctaa gctgattgat cagttagaaa
     2461 acgttaaaaa gacttttgct gaagaaccac aagaaccaaa aattgacaaa ggcaaaaatg
     2521 atgaattcaa cacaatgtct tctaacttag ataaagaaat tagcagaatt tctgaaaaga
     2581 gcacgcaatt gctatctgat acacaagaat caaaaacaat tgcagattca gttagtggac
     2641 aattaaatca attagataat aatgtgaata aactacatgc gacaggtcga gcattaggcg
     2701 taagagcgaa tgatttgaac cgtcaaatgg ctaaaaacga taaagataat gagttattcg
     2761 ctaaagagtt taaaaaagta ttacaaaatt ctaaagatgg cgacagacaa aaccaagcat
     2821 taaaagcatt tatgagtaat ccggttcaaa agaaaaactt agaaaatgtt ttagctaata
     2881 atggtaatac agaggtgatt tcaccgacat tattcgtatt attgatgtat ttactatcaa
     2941 tgattacagc atatattttc tatagttatg aacgtgccaa aggtcaaatg aatttcatta
     3001 aagatgatta tagtagtaaa aaccatcttt ggaataatgt cattacgtca ggtgttattg
     3061 gtacaactgg tttggtagaa ggattaattg tcggtttaat tgcaatgaat aagttccatg
     3121 tattagctgg ctatagagcg aaattcatct taatggtgat tttaactatg atggtctttg
     3181 tacttattaa tacgtattta ctaagacagg taaaatctat cggtatgttc ttaatgattg
     3241 ctgcattggg tctatacttt gtagctatga ataatttgaa agcggctgga caaggtgtga
     3301 ctaataaaat ttcaccattg tcttatatcg ataacatgtt cttcaattat ttaaatgcag
     3361 agcatcctat aggcttggca ctagtaatat taacagtact tgtgattatt ggttttgtac
     3421 tgaacatgtt tataaaacac tttaagaaag agagattaat ctaatgttga tgaatagcgt
     3481 gattgcttta acttttttaa cagcatctag caataatggc ggacttaata ttgatgtgca
     3541 acaagaagag gaaaagcgaa tcaataatga tttaaatcaa tatgatacaa cgctatttaa
     3601 taaagatagt aaagaagtca acgacgcgat tgctaagcag aaaaaagaac gacaacaaca
     3661 aataaaaaat gatatgtttc aaaatcaagc gagtcactcg actcgcttga atgaaactaa
     3721 aaaagtgtta ttttccaaat ctaacttaga aaagacttcg gagagtgata aaagccccta
     3781 tattcaaaac aagcaggaga aaaaaatatt cccgtacatt ttgatgtctg taggggcttt
     3841 tttgacttta ggatttgtca ttttttcaat tcataaaggg agacgaacga aaaatgaatc
     3901 agcacgtaaa agtaacattt gattttacta attataatta cggcacatat gacttagcag
     3961 taccagcata tttaccgata aaaaatttaa tagctttagt attggatagt ttggacattt
     4021 caatatttga tgtcaataca caaattaaag tgatgacgaa aggtcaatta cttgttgaaa
     4081 atgatcgact cattgattat caaatcgctg atggagatat tttgaagtta ctataggagg
     4141 aaaaatagat ggttaaaaat cataacccta aaaatgaaat gcaagatatg ttaacgcctt
     4201 tagatgctga agaagcagct aaaacaaaat tacgcttaga tatgagagag attcctaagt
     4261 cttcaattaa accagaacat tttcatttaa tgtacttatt agaacaacat tctccatatt
     4321 ttatagatgc tgaattaact gaactacgtg acagtttcca aatacattat gacattaatg
     4381 acaatcatac accttttgat aatattaaat catttactaa aaatgaaaaa ttacgatact
     4441 tactcaatat caaaaattta gaagaagtaa atcgtacacg ttacacattt gttttgacac
     4501 cagatgagtt atttttcaca agagatggat taccaattgc taaaacaaga ggtttacaaa
     4561 atgttgttga tccattaccg gtgtcagaag ctgaattttt aacaagatat aaagcactgg
     4621 ttatctgtgc attcaatgag aaacaatcat ttgatgcttt agttgaagga aacttagaac
     4681 tacataaagg aacgccattt gaaactaaag ttattgaagc ggcaacgtta gatttactaa
     4741 cggcattttt agatgaacag tatcagaaac aagaacaaga ttatagtcaa aattatgcat
     4801 atgtacgcaa agtagggcat accgttttca aatgggttgc tatcggcatg acaacgttaa
     4861 gtgtgttatt aattgcattt ttagcctttt tatatttttc agtaatgaag cataatgagc
     4921 gcattgaaaa aggataccaa gcatttgtaa aggatgatta tacgcaagta ctaaatacgt
     4981 atgatgattt agatggaaaa aaattagata aagaggcact ttacatttat gccaaaagtt
     5041 atatccaaac aaataaacaa ggtttagaaa aagataagaa agaaaattta cttaataacg
     5101 tgacaccaaa ttcaaacaaa gactacttat tatattggat ggaattagga caaggacatc
     5161 ttgatgaagc gattaatatt gccacttatt tagatgataa cgatattaca aagttagcgt
     5221 tgattaataa attaaatgag attaaaaata acggagattt atcgaatgat aaacgttctg
     5281 aagaaacgaa aaagtataac gataaattgc aagatatttt agacaaagaa aaacaagtta
     5341 aagatgaaaa agcgaaatct gaagaagaga aagcaaaagc gaaagatgag aaattaaagc
     5401 aacaagaaga gaacgaaaag aaacaaaaag aacaagcaca aaaagataaa gaaaaacgcc
     5461 aagaagctga aagaaaaaaa tagtatagga ctgaggcaaa gacaatgcat aaattgatta
     5521 taaaatataa caaacaattg aagatgctca atttgcgaga tggtaagaca tatactatta
     5581 gcgaagacga gcgtgcagat attacgttga aatcgttagg cgaagtcatt catttagaac
     5641 aaaataatca aggtacttgg caagcgaatc atacttctat taataaggtg cttgttagaa
     5701 aaggtgacct tgatgacatt acattacagc tttatacaga agctgattat gcatcatttg
     5761 cgtatccttc aattcaagat acgatgacaa ttgggccaaa tgcgtatgat gatatggtta
     5821 ttcaaagctt gatgaatgcc atcattatta aagattttca atcaatacaa gaatcacaat
     5881 acgtacgcat tgtgcacgat aaaaatacag atgtgtatat taactatgaa ctacaagagc
     5941 aattaacaaa caaagcttac attggtgatc atatttatgt tgaagggata tggctcgaag
     6001 tacaagctga tggtttaaat gtattgagtc agaatacagt ggcatcgtca ttaattcgct
     6061 taacacaaga gatgccacat gcacaggcag atgattacaa tacgtaccat cgttcgccaa
     6121 ggattattca ccgtgaaccg accgatgata ttaagattga aagaccgcca cagccaatac
     6181 agaagaacaa tacagtgata tggcgttcca ttataccgcc attagtaatg attgctttaa
     6241 ctgttgtcat ctttttagtg agaccaattg gtatttatat tttaatgatg attggtatga
     6301 gtacagtaac gatagtattt ggtattacaa cgtatttctc tgaaaagaaa aagtataaca
     6361 aagatgttga aaaacgagag aaagattaca aagcttattt ggataataaa tctaaagaaa
     6421 ttaataaagc gattaaagca caacgtttta gtttgaatta ccattatcca acggttgctg
     6481 aaattaaaga tatcgttgaa acgaaagcac caagaatata tgaaaaaaca tcgcatcatc
     6541 acgatttctt acattataag ttaggtattg cgaatgtaga aaagtcattc aaattagatt
     6601 accaagaaga agaatttaac caacgtcgtg atgaactatt cgacgatgct aaagaattgt
     6661 atgaatttta cacagatgta gaacaagcac cattaatcaa tgatttaaat catggaccaa
     6721 ttgcatatat tggtgcacga catctcattt tagaagaatt ggagaaaatg ctaatccaat
     6781 tgtcaacgtt ccatagttat catgatttag agtttctatt tgtgacacgt gaagatgaag
     6841 ttgaaacatt gaaatgggca cgttggttgc cacatatgac attgagaggt caaaacatta
     6901 gagggtttgt ttacaatcaa cgaacacgtg accaaatttt aacgtcaatt tatagcatga
     6961 ttaaagaacg tatccaagct gtgcgtgaac gcagcagaag taatgagcaa attattttca
     7021 caccgcaatt agtgtttgtc attacagata tgtcattaat tattgatcat gtcattttag
     7081 aatatgtaaa ccaagattta tcagaatatg gtatttcatt aatctttgtt gaagatgtga
     7141 ttgaaagttt gccagagcat gtagatacca ttattgatat caagtctcgt actgaaggcg
     7201 aactgattac gaaagaaaaa gaattagttc aattgaaatt tacacctgaa aatattgata
     7261 acgttgataa agaatatatc gcgagacgtt tggcgaattt gatacacgtc gaacatttga
     7321 aaaatgcaat tcctgatagt attacatttt tagagatgta taacgtgaaa gaagtagatc
     7381 agcttgatgt ggttaatcga tggagacaaa acgaaacata caaaacgatg gcagtacctt
     7441 taggtgtaag aggtaaagat gatattttat cattgaactt acatgaaaaa gcacacgggc
     7501 cacatggttt agttgctggt accactggtt cagggaaatc tgagattatc caatcataca
     7561 ttttatcttt agctattaat tttcatccac atgaagttgc gttcctattg attgactata
     7621 aaggtggggg tatggcgaac ttatttaaag atttagtcca tttagttggt acgattacaa
     7681 acttagatgg cgatgaagcg atgcgtgcct taacatcaat caaagccgaa ttgagaaaac
     7741 gtcaacgttt attcggagag catgatgtta accatattaa tcaataccat aagttattta
     7801 aagaaggtgt tgcaacagaa ccaatgccac atttattcat tatttccgat gagtttgccg
     7861 aattaaaatc agaacaacct gattttatga aagaacttgt atcaacggca cgtattggac
     7921 gttcgttagg tattcattta atacttgcga cacaaaaacc atcgggtgtt gttgatgacc
     7981 aaatttggtc taactctaaa tttaaattgg cattaaaagt acaagataga caagacagta
     8041 atgaaatttt aaaaacacca gatgcagcag acattacatt accaggtcgt gcgtatttac
     8101 aagttggtaa taatgaaatt tatgaattat tccaatctgc atggagtggt gcaacatatg
     8161 acatcgaagg cgataaatta gaagttgaag ataagacgat ttacatgatt aatgactatg
     8221 gtcaacttca agcaatcaac aaagacttga gtggacttga agatgaagaa acgaaagaaa
     8281 accaaaccga gttagaagcg attatcgatc atatcgaatc tattacaaca cgattagaaa
     8341 ttgaagaagt taagcgtcca tggttaccac cattaccaga aaatgtatat caagaagatt
     8401 tagtagaaac agatttcaga aaattatggt cagatgatgc aaaagaagtg gaattaacat
     8461 taggacttaa agacgtacca gaagaacaat atcaaggacc gatggtattg caattgaaaa
     8521 aagctgggca catcgcgtta atcggaagtc caggatatgg tagaacaacg ttcttacaca
     8581 acattatttt cgatgttgca agacaccatc gtcctgatca agcacacatg tacttgttcg
     8641 atttcggtac caatggtttg atgccagtca cagatatacc acatgttgct gattatttta
     8701 cagtagatca agaagacaag attgctaagg cgatacgtat atttaatgat gaaatcgatc
     8761 gtcgtaagaa gattttaagt cagtatcgtg ttactagtat ttctgaatat cgaaaattaa
     8821 ctggtgaaac aattccgtat gtctttattc ttattgataa ctttgacgca gtaaaagatt
     8881 cacctttcca agaagttttt gaaaatatga tgattaaaat gacacgtgaa gggctagcat
     8941 tagacatgca agtaacctta actgcttcaa gagctaacgc aatgaaaacg ccaatgtaca
     9001 ttaatatgaa aacgcgtatc gccatgtttt tatatgataa atcagaggtg tcgaacgtag
     9061 tagggcaaca aaaatttgcg gttaaagatg tagtgggtcg agcattgtta agtagtgatg
     9121 acaatgtatc attccatatt ggccaaccat ttaaacatga tgagactaaa tcatataatg
     9181 atcaaattaa tgatgaagta tcggcgatga cagaatttta taaaggtgaa acaccaaatg
     9241 atattcctat gatgccagat gaaattaaat atgaagatta cagagaatca ttaagcttac
     9301 cagatatagt tgccaatggt gctttaccaa ttggattaga ttatgaaggt gttacactac
     9361 aaaaaattaa attaactgaa ccagcaatga tttcatcaga aaatccgaga gaaattgcgc
     9421 atattgccga aattatgatg aaagaaattg acatattaaa tgaaaaatat gcgatttgta
     9481 tcgcagactc aagtggagag tttaaagctt ataggcatca agtggctaac tttgccgaag
     9541 aaagagaaga cattaaagcg attcatcaac taatgattga agacttaaaa caaagagaaa
     9601 tggacggtcc atttgaaaaa gattcacttt acattatcaa tgattttaaa acatatattg
     9661 attgcacgta tatcccggaa gatgatgtta aaaagcttat tacaaaagga ccagaacttg
     9721 gcttgaacat tttatttgtc ggcattcata aagaattaat agatgcttat gataaacaga
     9781 ttgatgttgc acgtaaaatg attaaccaat ttagtatagg tattcgtatt tcagaccaac
     9841 aattctttaa atttagattt attcaacgag aacctgttat taaagaaaac gaagcgtata
     9901 tggtcgcgaa tcaagcttat caaaagatta gatggtttaa atagtaatga attaaatagg
     9961 agggaggtat gttatgaatt ttaatgatat tgaaacaatg gtcaagtcga aatttaaaga
    10021 tattaaaaag catgctgaag agattgcgca tgaaattgaa gttcgttctg gatatttaag
    10081 aaaagctgaa caatataagc gattagaatt taatttaagt attgcactag atgatgttga
    10141 aagcacagca aaggacgtac aaactgcaaa atctagtgct aataaagaca gtgtatctgt
    10201 taaaggaaaa gcgcccaata cgttatatat cgaaaaaaga aatttgatga aacaaaagct
    10261 tgaaatgttg ggtgaagata tcgataaaaa taaagaatcc ctccaaaaag ctaagggaat
    10321 tgctggcgaa aaggcaagtg aatattttaa taaagcaatg aattaatatt gaggtgaaga
    10381 tatgggtgga tataaaggga ttaaagcaga tggtggcaag gtgaatcaag cgaaacaatt
    10441 agcggcaaaa atagctaaag atattgaagc atgtcaaaag caaacgcaac agctcgctga
    10501 gtatatcgaa ggtagtgatt gggaaggaca gttcgccaat aaggtgaaag atgtgttact
    10561 tattatggca aagtttcaag aagaattagt acaaccgatg gctgaccatc aaaaagcaat
    10621 tgataactta agtcaaaatc tagcgaaata cgatacatta tcaattaagc aaggacttga
    10681 tagggtgaac ccatgatgaa agatgttaag cgaatagatt atttttctta cgaagaatta
    10741 acaattttag gtggtagtaa attgcctctc gtaaattttg aattgtttga tccatcaaat
    10801 tttgaagaag ctaaagtggc tttaattgaa aaagaattag taacagagaa tgacaagtta
    10861 actgatgcag gttttaaagt ggctacatta gtaagagagt atattagcgc aattgtaaat
    10921 attcgaatta atgatatgta ttttgcacca tttagctatg aaaaagatga atatattttg
    10981 ttaagccggt ttaaaaataa tgggtttcaa atacgaatta tcaataaaga cattgcatgg
    11041 tggtcgattg tacaatcata tcctttattg atgagacaag aaaaatccaa tgattgggac
    11101 tttaaacaaa ttgacgatga aatattggag aacttaaata atgaaagtat cgatacgatt
    11161 gggcgtgttt tagaaattga aatctacaat catcaaggtg accctcaaca aagtttatat
    11221 aacatttatg aacaaaatga tttgttactc attcgatacc cattaaaaga taaagtgctg
    11281 aatgttcata ttggtgtcat taatacattt atacgagaat tatttggatt cgatactgat
    11341 gaaaatcata ttaataaagc agaggagtaa tgacgttgag tggaaaaatt agtgttaaag
    11401 ctgaaacgat tgcacatgtt gtaaaagaat tggaaatcat tagtcaaaag tatgatgaaa
    11461 tagctcaaaa ctttggaaaa atagcgcaat taaattacta cagtagtgaa aaagctgcac
    11521 attctatgga aaatggctat agtagtgctg caacagtcat tagtggtctc aaaggtccat
    11581 tgagtacact cggtggtggc gtcatgaatt cagcacaaaa gttctttgaa gcagatgaac
    11641 attggggtac ggaatttgcc aagctttact ataatattga gggataggtg catgacatga
    11701 caaaagatat tgaatatcta acagctgatt atgacaatga aaagtcatct atccaaagtg
    11761 taatagatgc aatagagggt caagacttct tagatgtaga tacaacaatg gatgatgcgg
    11821 taagcgatgt cagttcttta gacgaagatg gcgcaatatc attaacaagt agtgtagtag
    11881 gtccacaagg atctaaatta atggggtatt atcaaaatga gctatatgat tatgcatctc
    11941 aattagattc gaaaatgaaa gaaattattg acacgccatt tatagaagat atagataaag
    12001 cattcaaagg tataacgaat gttaaattgg aaaatatatt aataaaaaat ggcggtggtc
    12061 atggtagaga tacctatggg gcttctggga aaattgcaaa gggagatgcc aagaaaagtg
    12121 acagcgatgt ttatagcatc gatgaaatat taaaatcgga tcaagaattt gtaaaagtaa
    12181 ttgatcagca ttacaaagaa atgaaaaaag aagataagaa actatctaag agtgattttg
    12241 aaaaaatgat gactcaaggt gcttcttgtg attacatgac agtagctgaa gcggaagaga
    12301 tagaagagca aaagaaaaaa gaagaagcta tagagattgc agcactagct ggtatggtag
    12361 ttttatcttg tattaatcct gttgctggag cagtagctat tggtgcttat tccgcttatt
    12421 cagcagcaaa tgcagccaca ggaaaaaata ttgtaactgg aagaaagcta tctaaagaag
    12481 aacgaatcat ggaaggactt tcgcttattc cattgccagg tatgggcttc ctcaaaggtg
    12541 ctgggaaaag tttaatgaaa ttaggcttca aaggcggaga aaaatttgca gttaaaacag
    12601 gattgcaaaa gacaatgcaa caagcagtta gtcgtatttc acctaaaatg ggaatgatga
    12661 aaaacagtgt gttgaatcaa tctcgtaact ttgctcaaaa tactcatgtt ggacaaatgc
    12721 tgagtaacat gcgtggtcaa gcaactcata ctgttcaaca aagtagaaat tggattggac
    12781 aacaatcaca aaatgtcaaa cgaatagtga ataatggact tgataaagaa atagcacatc
    12841 catttaaaca acaacttgca ccagcgggaa tgggtggtat aaaatttgct gaaacaacta
    12901 ctttgagaaa catgggtcaa aacatgaaac gtgctgttac accacaaaat cacgtgacac
    12961 atggtccgaa agatagtatg gtgagaagtg aaggtaaaca tagtgtaagt agccatgaaa
    13021 taaatttatc aaaatatgtt gaatcaccaa actacaccaa ggtcaaatat ggagagcaat
    13081 atgcaagact tagacctaag aaactaaaag caaatataga atatacaaca ccaactggtc
    13141 acatatatcg gactgatcat aaagggcgca taaaagaagt ttatgtagac aatctttctc
    13201 taaaagatgg cggtcgtaat aaccatgcac aaagaactgt gggaggagag gatagattac
    13261 cagacgatga tggaggtcac ttaatcgcca gaatgtttgg tgggtcaaaa gacatagata
    13321 accttgtagc acaaagtaaa tatatcaacc gttcatttaa ggaaaatggt gattggtaca
    13381 agttggaaaa actatgggaa aaggcaatca agtctggaaa atcaatagaa aatattaaaa
    13441 tagaagttaa atataaaggt aatagtcaaa ggccgactat atttaaagtt gaatatgaaa
    13501 ttaatggtaa aagaaatatt agaagaatat taaataagta gaggtgccaa catgacattt
    13561 gaagagaagc ttagcaaaat atacaatgaa attgcgaatg agattagcaa tatgataccg
    13621 gtagagtggg aaaaagtata tacaatggct tatatagatg atggaggagg ggaggtattc
    13681 tttaattata ctaa
//
LOCUS       DAOBBH010000056        13661 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.056, whole genome shotgun sequence.
ACCESSION   DAOBBH010000056 DAOBBH010000000
VERSION     DAOBBH010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13661
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.056"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            545..1558
                     /locus_tag="P6L01_001210"
     CDS             545..1558
                     /locus_tag="P6L01_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDP3030288.1"
                     /translation="MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHM
                     DMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKGYFDIYADDNRLSEGIK
                     NKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAI
                     HLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLL
                     EPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQ
                     GQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKI
                     ILE"
     gene            1611..2621
                     /gene="gap"
                     /locus_tag="P6L01_001211"
     CDS             1611..2621
                     /gene="gap"
                     /locus_tag="P6L01_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDP3030289.1"
                     /translation="MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHL
                     LKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTD
                     KDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVA
                     KVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIG
                     KVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNASNESFGYTED
                     EIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAEL
                     SK"
     gene            2760..3950
                     /locus_tag="P6L01_001212"
     CDS             2760..3950
                     /locus_tag="P6L01_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDP3030290.1"
                     /translation="MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTI
                     QYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRSEKLEA
                     AIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHAS
                     NVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIAD
                     KIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSN
                     DAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTI
                     GVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKA
                     INNK"
     gene            4072..4833
                     /locus_tag="P6L01_001213"
     CDS             4072..4833
                     /locus_tag="P6L01_001213"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDP3030291.1"
                     /translation="MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAI
                     QLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRE
                     LFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQ
                     LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKP
                     NNIKEYMAQTDIDGALVGGASLKVEDFVQLLEGAK"
     gene            4836..6353
                     /locus_tag="P6L01_001214"
     CDS             4836..6353
                     /locus_tag="P6L01_001214"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDP3030292.1"
                     /translation="MAKKPTALIILDGFANRESEHGNAVKLANKPNFDRYYNKYPTTQ
                     IEASGLDVGLPEGQMGNSEVGHMNIGAGRIVYQSLTRINKSIEDGDFFENDVLNNAIA
                     HVNSHDSALHIFGLLSDGGVHSHYKHLFALLELAKKQGVEKVYVHAFLDGRDVDQKSA
                     LKYIEETEAKFNELGIGQFASVSGRYYAMDRDKRWEREEKAYNAIRNFDAPTYATAKE
                     GVEASYNEGLTDEFVVPFIVENQNDGVNDGDAVIFYNFRPDRAAQLSEIFANRAFEGF
                     KVEQVKDLFYATFTKYNDNIDAAIVFEKVDLNNTIGEIAQNNNLTQLRIAETEKYPHV
                     TYFMSGGRNEEFKGERRRLIDSPKVATYDLKPEMSAYEVKDALLEELNKGDLDLIILN
                     FANPDMVGHSGMLEPTIKAIEAVDECLGEVVDKILDMDGYAIITADHGNSDQVLTDDD
                     QPMTTHTTNPVPVIVTKEGVTLRETGRLGDLAPTLLDLLNVEQPEDMTGESLIKH"
     gene            6483..7787
                     /gene="eno"
                     /locus_tag="P6L01_001215"
     CDS             6483..7787
                     /gene="eno"
                     /locus_tag="P6L01_001215"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDP3030293.1"
                     /translation="MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTG
                     EHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGT
                     PNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDA
                     PIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTE
                     DAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVD
                     YLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIEN
                     GIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK"
     gene            8126..8584
                     /locus_tag="P6L01_001216"
     CDS             8126..8584
                     /locus_tag="P6L01_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030294.1"
                     /translation="MADRTNKEIKTGRFIATASIVFSILLIIHYFVSLDNATAKALLN
                     LTNQNTSDKAIDYILNSFRFTGIMYILAYLAGFITFWNRHTYVWWFMFAVYVSNSLFT
                     LINLSITIQAIKAAHGAYLTLPILIVIIGSVALAIYMLVVSIKRKSTFNR"
     gene            8651..8884
                     /gene="secG"
                     /locus_tag="P6L01_001217"
     CDS             8651..8884
                     /gene="secG"
                     /locus_tag="P6L01_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDP3030295.1"
                     /translation="MHTFLIVLLIIDCIALITVVLLQEGKSSGLSGAISGGAEQLFGK
                     QKQRGVDLFLNRLTIILSILFFVLMICISYLGM"
     gene            9000..9740
                     /locus_tag="P6L01_001218"
     CDS             9000..9740
                     /locus_tag="P6L01_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HDP3030296.1"
                     /translation="MQIKLPKPFFFEEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYT
                     SYAPQYEGHAAPPEEILKSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKL
                     SLNRDVKGIVTMCAPMGGKTEGAIYEGFLEYARNFKKYEGKNQETIDNEMDHFKPTET
                     LKELSEALDTIKDQVDEVFDPILVIQVENDNMIDPQSANYIYDHVDSDDKDIKWYSQS
                     GHVITIDKEKEQVFEDIYQFLESLDWSE"
     gene            9774..12131
                     /gene="rnr"
                     /locus_tag="P6L01_001219"
     CDS             9774..12131
                     /gene="rnr"
                     /locus_tag="P6L01_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDP3030297.1"
                     /translation="MNLKQSIEEIINQPEYEPMSVSDFQDALGLSSADSFRDLIKVLV
                     ELEQSGLIERTKTDRYQKKHSSKGHSKLIKGTLSQNKKGFAFLRPEDEDMEDIFIPPT
                     KINRALDGDTVIVEIHQSKGEHKGKIEGEVKSIEKHSVTQVVGTYSEARHFGFVIPDD
                     KRIMQDIFIPKGQSLGAVDGHKVLVQITKYADGSDNPEGHISAILGHKNDPGVDILSI
                     IYQHGIEIEFPDEVLQEAEAVPDHIENTEIKGRHDLRDELTITIDGADAKDLDDAISV
                     KKLANGNTQLTVSIADVSYYVTEDSALDKEAYDRATSVYLVDRVIPMIPHRLSNGICS
                     LNPHVDRLTLSCRMEIDASGRVVKHEIFDSVIHSDYRMTYDAVNQIITEKDPNIREQY
                     KEITPMLDLAQDLSNRLIQMRKRRGEIDFDISEAKVLVNEVGIPTDVQLRQRGEGERL
                     IESFMLIANETVAEHFSKLNVPFIYRVHEQPKSDRLRQFFDFITNFGIMIKGTGEDIH
                     PTTLQKVQEEVEGRPEQMVISTMMLRSMQQAHYDDVNLGHFGLSAEYYTHFTSPIRRY
                     PDLTVHRLIRKYLIEKSMDNKEVKRWEDKLPELAEHTSKRERRAIEAERDTDELKKAE
                     YMIQHIGDEFEGIVSSVANFGMFIELPNTIEGMVHIANMTDDYYRFEERQMALIGERQ
                     AKVFRIGDTVKVKVTHVDVDERLIDFQIVGMPLPKNDRSQRPARGKTRGKSLDKSKSD
                     DKGRKKKGKQRKGKNQRNNDKSGNSKHKPFYKDKSVKKKARRKKK"
     gene            12153..12617
                     /gene="smpB"
                     /locus_tag="P6L01_001220"
     CDS             12153..12617
                     /gene="smpB"
                     /locus_tag="P6L01_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDP3030298.1"
                     /translation="MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGS
                     ANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPLRSRKLLLHKREIFKLGEQTREI
                     GYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY"
     gene            12708..13069
                     /gene="ssrA"
                     /locus_tag="P6L01_001221"
     tmRNA           12708..13069
                     /gene="ssrA"
                     /locus_tag="P6L01_001221"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         4747 a         2041 c         2732 g         4141 t
ORIGIN      
        1 tgcacattat tgtaagctga ctttccgtca gcttctgtgt tggggccccg ccaacttgca
       61 cattattgta agctgacttt ccgccagctt ctgtgttggg gccccacccc aacttgcatt
      121 gcctgtagaa attggaaatc caatttctct atgttggggc ccctgacttt aattggaaaa
      181 agcttgttac aagtgcattt tcgttcggtc aactactact aatgtgactt ttcggattct
      241 agagcattgt tttatgtctt agtctcaaat aataagcaaa tgattagcga gtcagtataa
      301 gggtatacat ttgactacag cgaataaaat aaaacgtttt gttgaataac aaatcgaaaa
      361 tgtattgcaa gcgctttatc aaattattta gaaaatttaa gttttatgct tgcaattttt
      421 gaaatagaat agtactattg caagtgtaaa gaggttaatt tttgtcccac gcgggactta
      481 aaaaggcaac cactggttgt gacatatcct tatttacatt tataaatata aggaggaggt
      541 agtagtgaaa gacttattgc aagcacagca aaagcttata ccggatctca tagataaaat
      601 gtataaacgt ttttctattc ttactactat ctcaaaaaat cagcctgtcg gacgtcgaag
      661 tttaagcgaa catatggata tgactgaacg tgtactgcgt tctgaaacag atatgcttaa
      721 gaaacaagat ttgataaaag ttaagcctac cggaatggaa attacagctg aaggtgagca
      781 actgatttcg caattgaaag gttactttga tatctatgca gatgataatc gtctgtcaga
      841 aggtattaag aataaatttc aaattaagga agttcatgtt gttcctggtg atgctgataa
      901 tagtcaatct gttaaaacag aattaggtag acaagcaggt caattacttg aaggcatatt
      961 acaagaagac gcgatagttg ctgtaactgg cggatccacg atggcatgtg ttagtgaagc
     1021 aattcattta ttaccatata atgtattctt cgtaccagcc agaggtggac taggcgaaaa
     1081 tgttgtcttt caggcaaaca caattgcagc cagtatggca caacaagctg gcggttatta
     1141 tacgacgatg tatgtacctg ataatgtcag tgaaacaaca tataacacat tgttgttaga
     1201 gccatcagtc ataaacactt tagacaaaat taaacaagca aacgttatat tacacggcat
     1261 tggtgatgcg ctgaagatgg cgcatcgacg tcaatcacct gaaaaggtca ttgaacaact
     1321 tcaacatcat caagctgtcg gagaggcatt tggttattat tttgatacac aaggtcaaat
     1381 tgtccataag gttaaaacaa ttggacttca attagaagac cttgaatcaa aagactttat
     1441 ttttgcagtt gcaggaggca aatcgaaagg tgaagcaatt aaagcatact tgacgattgc
     1501 acccaagaat acagtgttaa tcactgatga agccgcagca aagataatac ttgaataaga
     1561 gataaaaagt ttaatacttt ttaaatatca ttttaaagga ggccattata atggcagtaa
     1621 aagtagcaat taatggtttt ggtagaattg gtcgtttagc attcagaaga attcaagaag
     1681 tagaaggtct tgaagttgta gcagtaaacg acttaacaga tgacgacatg ttagcgcatt
     1741 tattaaaata tgacactatg caaggtcgtt tcacaggtga agtagaggta gttgatggtg
     1801 gtttccgcgt aaatggtaaa gaagttaaat cattcagtga accagatgca agcaaattac
     1861 cttggaaaga cttaaatatc gatgtagtgt tagaatgtac tggtttctac actgataaag
     1921 ataaagcaca agctcatatt gaagcaggcg ctaaaaaagt attaatctca gcaccagcta
     1981 ctggtgactt aaaaacaatc gtattcaaca ctaaccacca agagttagac ggttctgaaa
     2041 cagttgtttc aggtgcttca tgtactacaa actcattagc accagttgct aaagttttaa
     2101 acgatgactt tggtttagtt gaaggtttaa tgactacaat tcacgcttac acaggtgatc
     2161 aaaatacaca agacgcacct cacagaaaag gtgacaaacg tcgtgctcgt gcagcggcag
     2221 aaaacatcat ccctaactca acaggtgctg ctaaagctat cggtaaagtt attcctgaaa
     2281 tcgatggtaa attagatggt ggtgcacaac gtgttcctgt agctacaggt tcattaactg
     2341 aattaacagt agtattagaa aaacaagacg taacagttga acaagttaac gaagctatga
     2401 aaaatgcttc aaacgaatca ttcggttaca ctgaagacga aatcgtttct tcagacgttg
     2461 taggtatgac ttacggttca ttattcgacg ctacacaaac tcgtgtaatg tcagttggcg
     2521 accgtcaatt agttaaagtt gcagcttggt atgataacga aatgtcatat actgcacaat
     2581 tagttcgtac attagcatac ttagctgaac tttctaaata attttagtat agtttttatt
     2641 caaatacgct agtgctcaga actatttagc attaattaaa gcttatgagt aagcggggag
     2701 cacaaacgct tctccgctta tttttatata aaatttccta attacaagga ggaaacacca
     2761 tggctaaaaa aattgtttct gatttagatc ttaaaggtaa aacagtccta gtacgtgctg
     2821 attttaacgt acctttaaaa gacggtgaaa ttactaatga caaccgtatc gttcaagctt
     2881 tacctacaat tcaatacatc atcgaacaag gtggtaaaat cgtactattt tcacatttag
     2941 gtaaagtgaa agaagaaagt gataaagcaa aattaacttt acgtccagtt gctgaagact
     3001 tatctaagaa attagataaa gaagttgttt tcgtaccaga aacacgcagc gaaaaacttg
     3061 aagctgctat taaagacctt aaagaaggcg acgtattatt agttgaaaat acacgttatg
     3121 aagatttaga cggtaaaaaa gaatctaaaa atgatccaga attaggtaaa tactgggcat
     3181 ctttaggtga tgtgtttgta aatgatgctt ttggtactgc gcatcgtgag catgcatcta
     3241 atgttggtat ttctacacat ttagaaactg cagctggatt cttaatggat aaagaaatta
     3301 agtttattgg tggcgtagtt aacgatccac ataaaccagt tgttgctatt ttaggtggag
     3361 caaaagtatc tgacaaaatt aatgtcatca aaaacttagt taacatagct gataaaatta
     3421 tcatcggcgg aggtatggct tatactttct taaaagcgca aggtaaagaa attggtattt
     3481 cattattaga agaagataaa atcgacttcg caaaagattt attagaaaaa catggtgata
     3541 aaattgtatt accagtagac actaaagttg ctaaagaatt ttctaatgat gccaaaatca
     3601 ctgtagtacc atctgattca attccagcag accaagaagg tatggatatt ggaccaaaca
     3661 ctgtaaaatt atttgcagat gaattagaag gtgcgcacac tgttgtatgg aatggaccta
     3721 tgggtgtatt cgagttcagt aactttgcac aaggtacaat tggtgtatgt aaagcaattg
     3781 caaaccttaa agatgcaatt acgattatcg gtggcggtga ttcagctgca gcagcaatct
     3841 ctttaggttt tgaaaatgac ttcactcata tttcaactgg tggcggcgcg tcattagagt
     3901 acctagaagg taaagaattg cctggtatca aagcaatcaa taataaataa taaagtgata
     3961 gtttaaagtg atgtggcatg tttgtttaac attgttacgg gaaaacagtc acaagatgac
     4021 atcgtgtttc atcacttttc aaaaatattt acaaaacaag gagtgtcttt aatgagaaca
     4081 ccaattatag ctggtaactg gaaaatgaac aaaacagtac aagaagcaaa agacttcgtc
     4141 aatgcattac caacattacc agattcaaaa gaagtagaat cagtaatttg tgcaccagca
     4201 attcaattag atgcattaac tactgcagtt aaagaaggaa aagcacaagg tttagaaatc
     4261 ggtgctcaaa atacgtattt cgaagataat ggtgcgttca caggtgaaac gtctccagtt
     4321 gcattagcag atttaggcgt taaatacgtt gttatcggtc attctgaacg tcgtgaatta
     4381 ttccacgaaa cagatgaaga aattaacaaa aaagcgcacg ctattttcaa acatggaatg
     4441 actccaatta tttgtgttgg tgaaacagac gaagagcgtg aaagtggtaa agctaacgat
     4501 gttgtaggtg agcaagttaa gaaagctgtt gcaggtttat ctgaagatca acttaaatca
     4561 gttgtaattg cttatgagcc aatctgggca atcggaactg gtaaatcatc aacatctgaa
     4621 gatgcaaatg aaatgtgtgc atttgtacgt caaactattg ctgacttatc aagcaaagaa
     4681 gtatcagaag caactcgtat tcaatatggt ggtagtgtta aacctaacaa cattaaagaa
     4741 tacatggcac aaactgatat tgatggggca ttagtaggtg gcgcatcact taaagttgaa
     4801 gatttcgtac aattgttaga aggtgcaaaa taatcatggc taagaaacca actgcgttaa
     4861 ttattttaga tggttttgcg aaccgcgaaa gcgaacatgg taatgcggta aaattagcaa
     4921 acaagcctaa ttttgatcgt tattacaaca aatatccaac gactcaaatc gaagcgagtg
     4981 gcttagatgt tggactacct gaaggacaaa tgggtaactc agaagttggt catatgaata
     5041 tcggtgcagg acgtatcgtt tatcaaagtt taactcgaat caataaatca attgaagacg
     5101 gtgatttctt tgaaaatgat gttttaaata atgcaattgc acacgtgaat tcacatgatt
     5161 cagcgttaca catctttggt ttattgtctg acggtggtgt gcacagtcat tacaaacatt
     5221 tatttgcttt gttagaactt gctaaaaaac aaggtgttga aaaagtttac gtacacgcat
     5281 ttttagatgg tcgtgacgta gatcaaaaat ccgctttgaa atacatcgaa gagactgaag
     5341 ctaaattcaa tgaattaggc attggtcaat ttgcatctgt gtctggtcgt tattatgcaa
     5401 tggaccgtga caaacgttgg gaacgtgaag aaaaagctta caatgctatt cgtaattttg
     5461 atgccccaac ttatgcaact gccaaagaag gtgtagaagc aagctataat gagggcttaa
     5521 ctgacgaatt cgtagtacca ttcatcgttg agaatcaaaa tgacggtgtt aatgatggag
     5581 atgcagtgat cttctataat ttccgacctg atagagcagc acaattatcg gaaatttttg
     5641 cgaacagagc attcgaaggc tttaaagttg aacaagttaa agacttattc tatgcaacat
     5701 tcactaagta taacgacaat atcgatgcgg ctatcgtttt cgaaaaagtt gatttaaata
     5761 atacaattgg tgaaattgca caaaataaca atttaacaca attacgtatt gcagaaactg
     5821 aaaaatatcc tcacgttact tactttatga gtggtggacg taacgaggaa tttaaaggtg
     5881 aacgccgtcg tttaattgat tcacctaaag ttgcaacgta tgacttgaaa ccagaaatga
     5941 gtgcttatga agttaaagat gcattattag aagagttaaa taaaggtgac ttggacttaa
     6001 ttattttaaa ctttgctaac cctgatatgg ttggacatag tggtatgctt gagccgacaa
     6061 tcaaagcaat cgaagcggtt gatgaatgtt taggtgaagt cgttgataag attttagaca
     6121 tggacggtta tgcaattatt actgctgacc atggtaactc tgatcaagta ttgacggatg
     6181 atgatcaacc aatgactacg catacaacga acccagtacc agtgattgta acaaaagaag
     6241 gcgttacact tcgagaaact ggtcgcttag gtgacttagc acctacatta ttagatttat
     6301 taaatgtaga acaacctgaa gacatgacag gtgaatcttt aattaaacac taatattgta
     6361 aaagatgtta agtaaacgct taatgacact tattttttga aaataatagt aatatcattt
     6421 tgttaaatga aagaataaag ctataataat tatagaataa ctatttaaag gagattataa
     6481 acatgccaat tattacagat gtttacgctc gcgaagtctt agactctcgt ggtaacccaa
     6541 ctgttgaagt agaagtatta actgaaagtg gcgcatttgg tcgtgcatta gtaccatcag
     6601 gtgcttcaac tggtgaacac gaagctgttg aattacgtga tggagacaaa tcacgttatt
     6661 taggtaaagg tgttactaaa gcagttgaaa acgttaatga aatcatcgca ccagaaatta
     6721 ttgaaggtga attttcagta ttagatcaag tatctattga taaaatgatg atcgcattag
     6781 acggtactcc aaacaaaggt aaattaggtg caaatgctat tttaggtgta tctatcgcag
     6841 tagcacgtgc agcagctgac ttattaggtc aaccacttta caaatattta ggtggattta
     6901 atggtaagca gttaccagta ccaatgatga acatcgttaa tggtggttct cactcagatg
     6961 ctccaattgc attccaagaa ttcatgattt tacctgtagg tgctacaacg ttcaaagaat
     7021 cattacgttg gggtactgaa attttccaca acttaaaatc aattttaagc aaacgtggtt
     7081 tagaaactgc agtaggtgac gaaggtggtt tcgctcctaa atttgaaggt actgaagatg
     7141 ctgttgaaac aattatccaa gcaatcgaag cagctggtta caaaccaggt gaagaagtat
     7201 tcttaggatt tgactgtgca tcatcagaat tctatgaaaa tggtgtatat gactacagta
     7261 agttcgaagg cgaacacggt gcaaaacgta cagctgcaga acaagttgac tacttagaac
     7321 aattagtaga caaatatcct atcattacaa ttgaagacgg tatggacgaa aacgactggg
     7381 atggttggaa acaacttaca gaacgtatcg gtgaccgtgt acaattagta ggtgacgatt
     7441 tattcgtaac aaacactgaa attttagcaa aaggtattga aaacggaatt ggtaactcaa
     7501 tcttaattaa agttaaccaa atcggtacat taactgaaac atttgatgca atcgaaatgg
     7561 ctcaaaaagc tggttacaca gcagtagttt ctcaccgttc aggtgaaaca gaagatacaa
     7621 caattgctga tattgctgtt gctacaaacg ctggtcaaat taaaactggt tcattatcac
     7681 gtactgaccg tattgctaaa tacaatcaat tattacgtat cgaagatgaa ttatttgaaa
     7741 ctgctaaata tgacggtatc aaatcattct ataacttaga taaataatca ttttaaacat
     7801 taagtactgc cataatttta gttgaggatt attatgtcgg tataaattga ataaagtttt
     7861 tgagttcacg cttaaaaagt tcacgcttgt atttataacc tgccacagag ttgagactgt
     7921 ggtaggtttt ttattttgaa gtattattca taacagacta ataatcatga ggtaactaat
     7981 aacacatatt taacttgtat tcttaaactg gtataataaa tttatgttga aatgaatatt
     8041 gtatgacagg gtattcactt ttattaaaag gtaaaattaa ataaaggttt tatagaaagt
     8101 atttaaatat atgaggagta aacaaatggc tgatagaacg aataaagaaa ttaaaacagg
     8161 acgctttatt gcaactgcat caatcgtatt ctcaatatta ttgattattc attactttgt
     8221 ttcgttggat aatgcgactg ccaaagcatt acttaattta acgaatcaaa acacttcaga
     8281 taaagcgatt gattacattt taaacagctt tagattcact ggtattatgt atattttggc
     8341 ttatctagca ggcttcatca ctttttggaa tcgacatact tatgtgtggt ggtttatgtt
     8401 tgcagtttat gtatcaaata gtttgtttac gttgattaat ttatcaatca caattcaagc
     8461 aataaaagct gcacacggtg cgtacttaac attgccaatt ttaatcgtta ttataggttc
     8521 ggttgcatta gcgatttata tgcttgttgt ttctatcaaa cgtaaaagta catttaatcg
     8581 ctagaaaatt gattttaaca ataaaaatat gatatactac ttgtcgtata taaggaacgg
     8641 aggacaattt atgcatacat ttttaatcgt attattaatc attgattgta ttgcattaat
     8701 aactgttgta ctactccaag aaggtaaaag cagtggactt tcaggtgcca tcagtggtgg
     8761 tgctgagcag ttattcggta aacaaaaaca acgtggcgtc gatttattct taaatagatt
     8821 aacaattatt ttatcaatat tattttttgt acttatgatt tgcataagtt atcttggtat
     8881 gtaaggtccg gcgatgtaaa tgtcgggctt ttttatttat aattaagaat gtaatagttt
     8941 aacaataagc tatgtaaaat atatagccta gttaagtatg caaagggagc gttagattta
     9001 tgcagataaa attaccaaaa cctttctttt ttgaagaagg caaacgtgcc gtgttattat
     9061 tacatggatt tacaggcaat tcgtctgatg tacgtcaatt aggtcgattt ttacaaaaaa
     9121 agggatatac ttcgtatgca ccacaatatg aaggtcacgc ggcaccacca gaggaaatac
     9181 tgaaatctag tcctttcgtt tggtttaaag atgcgttaga tggttatgat tatcttgttg
     9241 aacaaggtta tgatgaaatt gttgttgctg gtctatcatt aggtggggat tttgctttaa
     9301 aattaagctt aaatagagat gtaaagggta ttgtaacgat gtgtgcacca atgggtggca
     9361 aaactgaagg tgccatttat gaaggctttt tagagtatgc acgtaatttt aaaaagtatg
     9421 aaggtaaaaa tcaagagact attgataatg aaatggatca ttttaaacca actgaaactt
     9481 taaaagaact aagtgaagca ttagatacga ttaaagatca agttgatgag gtatttgacc
     9541 caattttagt gattcaagta gaaaacgaca atatgattga tccacaatcc gcaaattata
     9601 tatatgacca tgtagattct gatgacaaag acatcaagtg gtatagtcaa tcaggacatg
     9661 ttattacgat tgataaagaa aaagaacaag tatttgaaga tatttatcaa tttttagagt
     9721 cattagactg gtcagaataa aaagagattt taacattaga aaggaggggc ataatgaatt
     9781 taaagcaatc aatagaagag attattaatc aacctgaata tgaacctatg tcagtgtcag
     9841 attttcaaga tgcattaggt ttaagtagtg ccgactcatt tagagattta attaaggtgc
     9901 tcgtagaatt agaacaatca ggattaattg aacgtacaaa aacagacaga tatcaaaaaa
     9961 agcatagttc aaaaggtcat tcaaaattga taaagggaac gttaagtcaa aataaaaaag
    10021 gctttgcatt cttaagacct gaagatgagg atatggaaga tatatttatt cccccaacaa
    10081 aaattaatcg tgccttagat ggagatactg taattgtaga aatacatcaa tctaaaggtg
    10141 aacataaagg taaaattgaa ggggaagtta agtcgattga gaagcattca gtaactcaag
    10201 ttgttggtac gtatagtgaa gctagacatt tcggatttgt tattccagat gataaacgta
    10261 ttatgcaaga tattttcatt cctaaaggac aaagtttagg tgcagtagat gggcataagg
    10321 tacttgttca aattactaaa tatgctgatg gttcagataa tccagaagga catatttctg
    10381 ctattttagg acataaaaat gatcctggcg tagatatttt atctattatc tatcaacatg
    10441 gcatagaaat tgaatttcct gatgaagtgt tacaagaagc tgaagcagta cctgatcata
    10501 ttgaaaatac tgaaattaaa ggccgtcatg atttacgtga tgaattgaca atcacaattg
    10561 atggtgctga tgctaaagac ttagatgacg caattagtgt taaaaagtta gcgaacggta
    10621 atacgcagtt gactgtaagt attgctgatg tcagttatta tgtaactgaa gattcagctt
    10681 tagataaaga agcttatgat agagcgacaa gtgtatatct tgttgaccgt gtgattccga
    10741 tgattccaca tcgattaagc aatggtattt gttcattaaa tcctcatgtt gatcgtttaa
    10801 cgttaagctg tcgcatggaa atcgatgcta gtggtcgcgt tgttaaacat gaaatttttg
    10861 atagtgttat acattctgat tatcgaatga cgtatgatgc ggtaaatcag attattactg
    10921 aaaaggatcc taacattcgc gaacaatata aagaaattac gcctatgtta gatttagcac
    10981 aagatttatc taatcgtttg attcaaatga gaaaacgacg tggtgaaatc gattttgata
    11041 ttagtgaagc aaaagtatta gttaacgaag tcggtatacc aacagatgtt caattaagac
    11101 aacgtggcga gggtgaacgt ctaattgaat catttatgtt aattgcaaat gaaacagttg
    11161 ctgaacattt tagtaagtta aatgtacctt ttatttaccg agtgcatgag caacctaaat
    11221 cagatcgctt aagacaattc tttgatttta ttacaaactt tggcatcatg attaagggca
    11281 ctggcgaaga tattcatcca acaacacttc aaaaggttca agaagaagta gaaggtcgac
    11341 ctgaacaaat ggtcatttca acaatgatgt tacgttcaat gcaacaagcg cattatgatg
    11401 atgtgaactt gggacatttt ggcttatcag ctgaatatta tacgcatttt acatcaccaa
    11461 ttagacgtta tcctgattta acagttcatc gtttaatccg taagtattta attgagaaat
    11521 caatggataa caaagaagtg aagcgttggg aagacaaatt gcctgagtta gctgaacata
    11581 cttctaaacg tgaacgtcgt gctattgagg cagaacgtga tactgatgaa ttgaaaaaag
    11641 cagaatatat gattcaacat attggtgatg aatttgaagg tattgtcagc tcagtagcta
    11701 acttcggtat gttcattgaa ttgccaaata cgatagaagg tatggttcat attgcgaata
    11761 tgactgatga ttattaccgt tttgaagagc gtcaaatggc attaattggt gagcgtcaag
    11821 ctaaagtatt tagaattggt gacacagtta aggttaaagt gacgcatgtt gatgtagatg
    11881 aacgattaat tgattttcaa attgttggaa tgcctttacc taaaaatgac cgctcacagc
    11941 gaccagcaag aggtaaaacg cgtggcaaat ctttagataa atcgaaatct gatgataagg
    12001 gtcgtaagaa aaaaggtaag caacgtaaag gtaaaaacca acgtaataat gataaatcag
    12061 gtaatagtaa gcataagcca ttttataaag ataaaagtgt gaaaaagaaa gcacgtcgta
    12121 agaaaaaata agcagcaatg aggtgagtat gaatggctaa gaagaaatca ccaggtacat
    12181 tagcggaaaa tcgtaaagca agacatgatt ataatattga agatacgatt gaagcgggaa
    12241 tcgtattaca aggtacagaa ataaaatcga ttcgccgagg tagtgctaac cttaaagata
    12301 gttacgcgca agttaaaaac ggtgaaatgt atttgaataa tatgcatata gcaccatacg
    12361 aagaagggaa tcgttttaat cacgatcctc ttcgttctcg aaaattatta ttgcataaac
    12421 gtgaaatctt taaattaggt gaacaaactc gagaaattgg ttattcgatt gtgccgttaa
    12481 agctttattt gaagcatgga cattgtaaag tattacttgg tgttgcacga ggtaagaaaa
    12541 aatatgataa acgtcaagct ttgaaagaaa aagcagtcaa acgagatgtt gcgcgcgata
    12601 tgaaagcccg ttattaagcg atttagttgc ttaatcgggc tatatttgat atagttatat
    12661 gtgcttttgt aaattacaaa agtatgattt gtttgattta ttatttcggg gacgttcatg
    12721 gattcgacag gggtcccccg agctcattaa gcgtgtcgga gggttggctt cgtcatcaac
    12781 acacacagtt tataataact ggcaaatcaa acaataattt cgcagtagct gcctaatcgc
    12841 actctgcatc gcctaacagc atttcctatg tgctgttaac gcgattcaac cttaatagga
    12901 tatgctaaac actgccgttt gaagtctgtt tagaagaaac ttaatcaagc tagcatcatg
    12961 ttggttgttt atcacttttc atgatgcgaa acctttcgat aaactacaca cgtagaaaga
    13021 tgtgtatcag gacctctgga cgcgggttca aatcccgccg tctccatatt tgtagcctac
    13081 aacctttgtg gatgtaggct tttttattat gtgtttttca ggggataatg cattgcagaa
    13141 tttgttgtga gtattgatat agcagtgttt gtataggtgt ttatttgatg gaggaaagag
    13201 taataagtga ttatgaatta gtttttgaga tataagggga cagtgatgtg tgtcaaataa
    13261 gtgtcaaaaa agttggattc tgagttttac attcaacatt gttcatgaag aaacttcttt
    13321 atacgcaaaa aattctccat gttatatatg tcaatataaa aatgtgaatc gtctacactt
    13381 aattggataa atggctactg aaaaagaact tttcattttt gttacgtcac taagtgggtg
    13441 tagttataaa gagatgagcc gagttttgat attttcatta gaatcaatat gcctattaac
    13501 acaatcagca atagttgacg agacggaaat aaaagaagtc gtagttaaga aatgcatttc
    13561 acaacatacc attgtagcca tttttattgt tttggatgat aaactctttt tggaattttt
    13621 agtttttata atttgcaact acactacttc ttttactaat a
//
LOCUS       DAOBBH010000057        13564 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.057, whole genome shotgun sequence.
ACCESSION   DAOBBH010000057 DAOBBH010000000
VERSION     DAOBBH010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13564
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.057"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(236..2845)
                     /gene="adhE"
                     /locus_tag="P6L01_001222"
                     /gene_synonym="adhC"
     CDS             complement(236..2845)
                     /gene="adhE"
                     /locus_tag="P6L01_001222"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDP3030299.1"
                     /translation="MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEID
                     HIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIG
                     EDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKR
                     AAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTG
                     KPALGVGPGNVPSYIEKTAHIKRAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTN
                     EFKAHQAYFVKKDELQRLENAIMNEQKTGIKPDIVGKSAVEIAELAGIPVPENTKLII
                     AEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETL
                     QKDFGLRMKACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLN
                     IKTIAKRRNNTQIFKVPAQIYFEENAIMSLTTMDKIEKVMIVCDPGMVEFGYTKTVEN
                     VLRQRTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDAAKAMWM
                     FFEHPETSFFGAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSET
                     NVKYPLADFALTPDVAIINPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGL
                     SLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYG
                     IPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALV
                     ESLAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISE
                     IKDIIQTSYDYKQ"
     gene            complement(3230..4405)
                     /locus_tag="P6L01_001223"
     CDS             complement(3230..4405)
                     /locus_tag="P6L01_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDP3030300.1"
                     /translation="MFIGKNLEYVRKLNALSRKELSEKINVSEQAIWQYETKNMMPEI
                     SKIYDMTSIFNVKSSYFISEQPEELLINSVDKHSIAFRAKNYKVSTKLLNKQYYQAMY
                     LSNLTSYLFSFVTIPDNIILSLINNLDELLNGNLESLNKKESIKEIAKVVRAKILQDE
                     SNEALLFMLEKAGIVIYEKRINDSIDAYSFWSKDQTPFIILGTNRGVAVRRNFDLAHE
                     LGHLVLHRHIQFDLLSPEEYKTIEHEADIFASEFLLPEEAFKKDFDQMTKKSNPDYLA
                     VLKEKWYVSIQAIAMRAYYLGLMSSTQYRYFWASLNKKGYKSKEPLDDVIEMSRPVKM
                     NSLLKLYFDRNILTPQKLLNYLKVDETFLNHLAGINLKLFKDYVNENREYNITNLYK"
     gene            complement(4411..5178)
                     /locus_tag="P6L01_001224"
     CDS             complement(4411..5178)
                     /locus_tag="P6L01_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030301.1"
                     /translation="METIKQEVRESIVKGIIAGYKDYVNERNEVKKRMVISDAYAFTK
                     SNHIESQVAKHLENFVKYIKENAGPSWKYLKFIFNKDEEKNNIMFILKNEDYFDEKNV
                     SVGKSLVADKNPKSKNYLEVLMAKNRDINFGTVGEDFEIGHQMTADSILFNIKEGSNR
                     DINSYFLIITYRIDKESKQLAAIKQWLPNPETNSAIMVDDLTELIEKVIVEREDYHID
                     EEELEVLKNDGELELIDVEHAFGISIDEGNDMIESER"
     gene            complement(5335..6870)
                     /locus_tag="P6L01_001225"
     CDS             complement(5335..6870)
                     /locus_tag="P6L01_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDP3030302.1"
                     /translation="MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRR
                     QYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDFYNQMAFDFGTLGNHEFNYGLPY
                     LKDTLRRLNYPVLCANIYENDSTLTDNGVQYFQVGDQTVGVIGLTTQFIPHWEQPEHI
                     QSLTFHSAFETLQQHLPEMKRHADIIVVCYHGGFEKDLENGTPTEVLTGENEGYAMLE
                     AFSKDIDIFITGHQHRQIAERFKQTAVIQPGTRGTTVGKVVLSTDEYENVSVESCELL
                     PVIDDSTFTIDEDDQHLRKQLEDWLDYEITTLPYDMTINHAFEARVAPHPFTNFMNYA
                     LLEKSDADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGAKLKEAIERSA
                     EYFDVQNDEVSVSADFLGPKPQHFNYDIYGGVSYTIHVGRPKGQRVSNMMIQGHAVDL
                     KQTYTICVNNYRAVGGGQYDMFIDAPVVKDIQVEGAQLLIDFLSNNNLMRIPQVVDFK
                     VEK"
     gene            complement(6921..8465)
                     /locus_tag="P6L01_001226"
     CDS             complement(6921..8465)
                     /locus_tag="P6L01_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDP3030303.1"
                     /translation="MKKIYKSLTVSAIVATVSLSALPQSLAITHESQPTKQQRTVLFD
                     RSHGQTAGAADWVSDGAFSDYADSIQKQGYDVKAIDGHSNITEASLKSSKIFVIPEAN
                     IPFKESEQAAIVNYVKQGGNVVFISDHYNADRNLNRIDSSEAMNGYRRGAYEDMSKGM
                     NAEEKSSTAMQGVKSSDWLSTNFGVRFRYNALGDLNTSNIVSSKESFGITEGVKSVSM
                     HAGSTLAITNPEKAKGIVYTPEQLPAKSKWSHAVDQGIYNGGGKAEGPYVAISKVGKG
                     KAAFIGDSSLVEDSSPKYVREDNGEKKKTYDGFKEQDNGKLLNNITAWMSKDSDGKSL
                     KASGLTLDTKTKLLDFERPERSTEPEKEPWSQPPSGYKWYDPTTFKAGSYGSEKGADP
                     QPNTPDDHTPPNQNVKISFDIPQNVSVNEPFEVTIHLKGFEANQTLENLRVGIYKEGG
                     RQIGQFSSKDNDYNPPGYSTLPTVKADENGNATIKINAKVLESMEGSKIRLKLGDKTL
                     ITTDFK"
     gene            8694..9650
                     /locus_tag="P6L01_001227"
     CDS             8694..9650
                     /locus_tag="P6L01_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDP3030304.1"
                     /translation="MKNFKYLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGY
                     KPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVD
                     VGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSL
                     KDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDV
                     DAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFID
                     IAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK"
     gene            9845..10618
                     /gene="phnC"
                     /locus_tag="P6L01_001228"
     CDS             9845..10618
                     /gene="phnC"
                     /locus_tag="P6L01_001228"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDP3030305.1"
                     /translation="MSQIEFKNVSKVYPNGHVGLKNINLNIEKGEFAVIVGLSGAGKS
                     TLLRSVNRLHDITSGEIFIQGKSITKAHGKALLEMRRNIGMIFQHFNLVKRSSVLRNV
                     LSGRVGYHPTWKMVLGLFPKEDKIKAMDALERVNILDKYNQRSDELSGGQQQRISIAR
                     ALCQESEIILADEPVASLDPLTTKQVMDDLRKINQELGITILINLHFVDLAKEYGTRI
                     IGLRDGEVVYDGPASEATDDVFSEIYGRTIKEDEKLGVN"
     gene            10620..11420
                     /gene="phnE"
                     /locus_tag="P6L01_001229"
     CDS             10620..11420
                     /gene="phnE"
                     /locus_tag="P6L01_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDP3030306.1"
                     /translation="MPLEIPTKYDSLLKKKVSLKTSFTFMLIIVLIIWSFIYTGFNFG
                     DLMIGIPQIGDLFKQMIPPDFEYLQQITTPMLDTIRMAIVSTVLGSIVSIPIALLCAS
                     NIVHQKWISIPSRFILNIVRTIPDLLLAAIFVAVFGIGQIPGILALFILTICIIGKLL
                     YESLETIDPGPMEAMTAVGANKIKWIVFGVVPQAISSFMSYVLYAFEVNIRASAVLGL
                     VGAGGIGLFYDQTLGLFQYPKTATIILFTLVIVVVIDYISTKVRAHLA"
     gene            11417..12232
                     /gene="phnE"
                     /locus_tag="P6L01_001230"
     CDS             11417..12232
                     /gene="phnE"
                     /locus_tag="P6L01_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDP3030307.1"
                     /translation="MTQEIAKYNVHTKAHKRKLIKRWLIAIVVLAIIIWAFAGVPSLE
                     LKSKSLEILKSIFSGLFHPDISYIYIPDGEDLLRGLLETFAIAVVGTFIAAIICIPLA
                     FLGANNMVKLRPVSGVSKFILSVIRVFPEIVMALIFIKAVGPGSFSGVLALGIHSVGM
                     LGKLLAEDIEGLDFSAVESLKASGANKIKTLVFAVIPQIMPAFLSLILYRFELNLRSA
                     SILGLIGAGGIGTPLIFAIQTRSWDRVGIILIGLVLMVAIVDLISGSIRKRIV"
     gene            complement(12361..13539)
                     /gene="deoB"
                     /locus_tag="P6L01_001231"
     CDS             complement(12361..13539)
                     /gene="deoB"
                     /locus_tag="P6L01_001231"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDP3030308.1"
                     /translation="MTRPFNRVHLIVMDSVGIGEAPDAADFKDEGSHTLRHTLEGFDQ
                     TLPNLEKLGLGNIDKLPVVNAVEQPEAYYTKLSEASVGKDTMTGHWEIMGLNIMQPFK
                     VYPNGFPEELIQQIEEMTGRKVVANKPASGTQIIDEWGEHQMKTGDLIVYTSADPVLQ
                     IAAHEDIIPLEELYDICEKVRELTKDPKYLIGRIIARPYVGEPGNFTRTSNRHDYALK
                     PFGKTVLDHLKDGGYDVIAIGKINDIYDGEGVTEAVRTKSNMDGMDQLMKIVKKDFTG
                     ISFLNLVDFDALYGHRRDKPGYAQAIKDFDDRLPELFSNLKEDDLVIITADHGNDPTA
                     PGTDHTREYIPVIMYSPKFKGGHALESDTTFSSIGATIADNFNVTLPEFGKSYLKELK
                     "
BASE COUNT         4211 a         2483 c         2094 g         4776 t
ORIGIN      
        1 catattcatt tatatgaaat taaaaataat tatactctaa agtagtaata gtttgcgaaa
       61 tgagtatcaa tcttaatatt ttctttattt aaaaaaatga ttgtctagtt tgtatctctg
      121 aagatttggc aataaataaa agccgataac cgtaaaatga ttatcgactt aaagtttatg
      181 tggcattttt tacttttgta atttcaggtg agttatatga ttattatcag atagattatt
      241 gcttataatc atatgatgtt tgaatgatat ctttgatttc actgattagt gcttctttag
      301 gattagcagt tgtacattga tcttcaaatg cgagctctgc cattctatca attgattcat
      361 ttaattcttc ttcagacaca ccttgtgatt tcaaattcat ttcaattccg actgattgac
      421 ctaattcgta gacagcttta gctaatgatt ctacgagtgc ttctgtcgta ttccctttta
      481 atcctaagaa tttggcaata tctgcataat ctgtatctgc tctgaagaac tcatatttag
      541 ggaataatgc atgtttttgc gggtctttgg cattataacg gataatatgc ggtagtaata
      601 tcgcattcgc tctaccatgc ggaataccat attcgccacc aattttatgt gcaattgagt
      661 gtgcaatgcc taagaatgca tttgcaaatg ccataccagc caaagttgat gcgttatgca
      721 ttttctctct tgaaacttta tcaccctttt caacagatga ttttaaatat tcgaacgtca
      781 atttaatcgc ttgtagactc aaacctcttg tatagtctga agccattaca gatacatatg
      841 attccattgc atgcgttagt acatccattc ctgtatctgc tgtaacgctt tttggcacac
      901 tcatcacaaa ttgagggtta ataattgcaa cgtcaggtgt taaagcaaaa tcagccaacg
      961 gatattttac atttgtttca ctatctgtga taactgcaaa tggtgttact tctgaacctg
     1021 tacctgatgt cgtagggata caaatgaacg tcgcattttc aggcatgcct attttataag
     1081 tacgtttacc gatgtctagg aacttttgtt tagcaccgaa gaatgatgtc tcagggtgtt
     1141 caaagaacat ccacattgct tttgcagcat ccatcgctga accaccacca agtgcaatga
     1201 ttgtatctgg ttggaaatca accatcattt ccagaccttt atatactgta ttagttgatg
     1261 ggttcggttc gacttcgcta aatattttaa tttgaggctg ttccgttctt tgtcttaata
     1321 cattctcaac tgtttttgta taaccgaatt ctaccatacc agggtcacag acaatcatca
     1381 ctttttcaat cttgtccatt gttgttagac tcatgattgc attttcttca aaataaattt
     1441 gagcaggcac cttgaaaatt tgagtattat tacgtcgttt agcaatcgtt ttaatgttta
     1501 ataaatctgt cgcactaaca ttatgtgaaa ttgagtttct accgtacgaa ccacaaccta
     1561 atgttaaaga cggaatcaat tcgttataca tatcaccaat acctccaacc gctgatggtg
     1621 tatttacaag tacacgacaa gctttcattc ttagtccaaa atctttttgt aatgtttcat
     1681 cttctgtatg gataacggct gtgtgtccta atccaccaaa atgtagtgtg tcttcacaaa
     1741 tttgaaatgc ttgttttgta gattgggctt ttactaaggc taatactgga gataattttt
     1801 cacgagataa cggatagtct gaacctacac cgctaatttc ggctatgata agttttgtat
     1861 tttcggggac aggtatacct gctaattcag ctatttcaac tgcagattta ccgacaatat
     1921 caggcttaat acctgttttt tgttcattca taattgcatt ttctaagcgt tgtaattcat
     1981 cttttttaac aaagtatgct tgatgtgctt taaattcatt agtaacatct ttataaattt
     2041 ctttatcaat gactacaact tgttcagaag cacaaatcat accattatca aatgtttttg
     2101 aaccaatgat atcatttact gcacgtttaa tgtgtgctgt tttttcaatg taagacggca
     2161 cgttacctgg tcccacacct aatgccggtt tgccagttga atatgcagac ttaaccatgc
     2221 ccgaaccacc tgttgctaga actaatgcaa tacctttgtg attcattaat tgttttgttg
     2281 cttcgataga aggcacttca atccactgaa taatatcttt aggtgcacct gccttcattg
     2341 ccgcttctaa tacaacttct gctgcacgct tcgacgattc ttgtgcactt ggatggaatg
     2401 caaaaatgat tggatttcct gtcttaattg caatcatcgc tttaaaaata gttgtcgacg
     2461 taggatttgt tgttggcgta acaccacaaa taacaccaat tggttccgct acatacgtta
     2521 atcctttttc tttatcttca ccaataatcc ctactgtctt attgtctttt attgaattcc
     2581 atatatattc agaagcgtat aaatttttaa tcgctttatc ttcgtatata cctcttccag
     2641 tttcttcatg tgctaatttt gctagcacca tatgttgatc aacagctgct aagctcattt
     2701 gatgaacaat atgatcaatt tcttcttgtg actttttaga taatgcttct aatgcttttt
     2761 tccctttgtc agctagagca tcaatcataa ttgccacttc ttgttctttc gatccacgat
     2821 tttctttttc aggtatagtt aacatataca accactcctt tatactttgt gaattatttc
     2881 acaaacatta tagtacatgt ctctcaggat ataaagaaaa ttctatacaa aaaagtttaa
     2941 tttcgaatat tatttgaaca aatatcaaat tttaaaataa atgttttcat gaaatcattg
     3001 ttatttcggt gtttttagaa tgattttata atcataattt tttcaatgac ataatttatt
     3061 cataatttta tatttaattc tgaatattct ttaatagcct aaaaagcttg ttatatcgca
     3121 caaaacacaa cattaaaagg tccttgtcgc tacctatcaa cagatgttac acaaaaaaac
     3181 tcgaactgaa atgatgatct tttcagctcg agttttaaat attggtgcct tatttatata
     3241 gattcgttat attatattct ctattttcat taacataatc cttaaagagt tttaaattaa
     3301 tacctgctag atgattcaaa aatgtttcat cgacttttaa ataattcaat aatttttgtg
     3361 gtgtcagtat atttctatca aaatacaact ttaataaact attcattttg acaggacgtg
     3421 acatttcaat cacgtcgtct aaaggctctt tacttttata gcctttttta tttaatgatg
     3481 cccaaaaata tctgtactga gtactagaca ttaggcctag ataataagct ctcattgcaa
     3541 ttgcttgtat agaaacatac catttttctt tcaatacagc taaataatca ggatttgatt
     3601 ttttggtcat ttgatcaaag tcttttttaa acgcttcttc aggtagtaaa aattcagagg
     3661 cgaaaatatc tgcctcatgt tcaattgtct tatattcttc aggagataat aagtcgaatt
     3721 gaatatgacg atgtagaacg agatgtccca gctcatgtgc aagatcaaaa ttccttctaa
     3781 ctgctacccc cctatttgta cccagtataa taaaaggtgt ctgatcttta ctccaaaaac
     3841 tatatgcgtc tatgctatca ttaattcgct tttcgtatat aactataccc gctttttcaa
     3901 gcataaataa tagtgcttcg ttgctttcat cttgaagaat tttagccctc acaactttag
     3961 caatttcttt tatgctttct tttttattta gtgattctaa attgccattc aacaattcat
     4021 ctaaattatt tattaaagat aaaataatat tatccggtat cgttacaaaa gaaaataagt
     4081 agctagttaa attagataaa tacattgctt gataatattg cttatttaat agtttcgtag
     4141 atactttata attttttgct ctaaaagcta tactatgttt atcgacagaa tttattagga
     4201 gctcttcggg ctgctcgcta ataaagtagc tagatttaac attaaaaata gatgtcatat
     4261 cataaatttt actaatttct ggcatcatat tttttgtttc atattgccaa attgcttgtt
     4321 cactcacatt tattttttct gataattctt ttctacttag agcattaagc tttctcacgt
     4381 attctaaatt cttgccaata aacattaact tcacctttca ctttcaatca tatcgttacc
     4441 ttcatcgata ctgataccaa atgcatgttc cacatctatg agttctaact ctccatcgtt
     4501 tttaagtact tctaactctt cttcgtcaat atgataatct tctctttcta ctattacttt
     4561 ttctattaac tctgtcaaat catcaaccat aattgcagaa tttgtttctg gatttggtaa
     4621 ccattgtttg attgcagcta attgctttga ttctttatca atacgatatg taataattaa
     4681 aaaataagaa ttaatatctc tattactgcc ttcttttata ttaaataata tactatctgc
     4741 agtcatttga tgcccaattt caaaatcttc tccaacagta ccaaaattaa tgtccctatt
     4801 cttagccata agtacctcta ggtagttttt actttttgga tttttatcag caactaaact
     4861 cttccctacg cttacatttt tttcatcaaa gtaatcttca tttttcaata taaacatgat
     4921 attatttttc tcttcatctt tattaaatat aaactttaaa tacttccatg atggtccagc
     4981 attttcttta atatacttaa caaaattttc caaatgctta gcaacttgac tttcaatatg
     5041 attagattta gtaaaggcat aggcatcact aatcaccata cgtttcttga cttcatttct
     5101 ctcattaaca tagtccttat atcccgcgat aattccttta acaatagatt ctcttacttc
     5161 ttgtttaatt gtttccaaat taatcacttc ataatattaa gttatataaa atataagcct
     5221 cattttttgt aaaatcaaca tttttcttaa agtatattga cgtttaagag tggttagttt
     5281 aaacaaaaac aaaaacttac ccgattaaaa tcaagtaagt ttttcatata tccgtcactt
     5341 ttcaacttta aaatcaacaa cttgcgggat acgcatcaaa ttattatttg ataaaaaatc
     5401 aataagtaat tgtgcgcctt caacttgaat atctttcaca actggcgcgt cgataaacat
     5461 atcatactga ccaccgccaa ctgcacgata attatttaca caaattgtat atgtctgctt
     5521 taaatcaact gcgtgacctt gaatcatcat attgctcaca cgttgtccct ttggtcttcc
     5581 aacatgaatg gtataactta cgccaccata tatatcataa ttaaagtgtt gtggtttggg
     5641 tccaaggaag tctgcgctca cacttacttc atcattttgc acgtcaaaat attctgctga
     5701 tcgttcaatg gcttctttaa gtttggcacc acttacagct aaaactttaa atgtatttgg
     5761 aaatgggtaa ttgttaataa catctcgcat cgtcacgact tgcttgaaac cactagcaga
     5821 atcaaacaaa gctgtacagg caacatctgc gtcacttttt tctaataaag cgtaattcat
     5881 aaaattagta aaaggatgcg gtgccacacg tgcctcaaat gcatgattaa tcgtcatatc
     5941 atatggcaat gtagtaattt cgtaatctaa ccagtcctct aactgctttc gtaaatgttg
     6001 gtcatcttca tcaatagtaa atgtggaatc atctataaca ggaagtaatt cacatgattc
     6061 aacggataca ttttcatatt catcagtact caagacaact ttgcctacag ttgtacctct
     6121 cgtaccaggt tgaatcacag ccgtttgctt aaatctttca gcaatttgtc gatgttggtg
     6181 acccgtaata aagatatcta tatctttaga aaacgcttct aacatggcat atccttcatt
     6241 ttcacccgtt aatacttcgg tcggcgtacc attttctaaa tccttttcaa atccaccatg
     6301 gtaacaaacc acaatgatat ctgcatgtcg cttcatttca ggtaagtgtt gttgaagtgt
     6361 ttcaaaagca ctatgaaacg taagtgactg aatatgctct ggttgttccc aatggggaat
     6421 aaattgtgtc gttaaaccta tcacaccaac cgtttgatct ccaacctgaa aatactgcac
     6481 accgttatca gtcaatgtac tatcattttc ataaatatta gcgcacaaaa ctggataatt
     6541 gagtctgcgt aaagtgtctt ttaagtatgg caatccataa ttaaattcat gattaccaag
     6601 cgtaccaaag tcgaatgcca tttgattata aaaatcaact aaaggctggc tactgccgct
     6661 atgcgcgatt aagtaattac aaaatggtga cccttgcaaa aaatcaccat tatctatttt
     6721 aaaactttgg tcatactgcc ttctgtcttg ttctataaca tgattcgcta gtaacaatcc
     6781 cataggttga tattgatttc tactcgtaaa atctgttggg aaaatataac catgtacgtc
     6841 gctcacgaca taaaatgcta tgtttgacat cctcactcac tccttcaatc acaaacatct
     6901 ttcttatttc tattatatat ttatttgaag tctgttgtaa tcaaggtttt gtcaccgagt
     6961 tttaaacgaa tctttgaacc ttccatactt tcaagtactt tagcattgat cttaattgta
     7021 gcatttccgt tttcatctgc tttaactgtt ggcaaagtac tgtaacctgg tgggttataa
     7081 tcgttatctt tacttgaaaa ttgtccgatt tgacgtccgc cttctttgta aataccaact
     7141 ctaagatttt caagtgtttg atttgcttca aatcctttta aatgtattgt cacttcaaat
     7201 ggctcattta cagaaacatt ttgcgggata tcaaatgata tttttacgtt ctgatttggt
     7261 ggcgtatgat catctggtgt gtttggctga ggatccgcgc ctttttcact gccataacta
     7321 cctgctttaa atgttgttgg gtcataccat ttataaccac tcggcggttg tgaccatggc
     7381 tctttttcag gctcagttga acgctctggt cgttcaaaat caagcaactt agtctttgta
     7441 tctaatgtta gtccactcgc cttaagtgat ttcccatcac tatctttaga catccaagcc
     7501 gttatattat ttaatagctt accgttgtct tgttctttaa aaccatcata tgttttcttc
     7561 ttttctccat tatcttctct tacatatttg ggcgaactat cttccacaag tgatgaatca
     7621 ccgataaatg ctgctttacc ttttccaact ttagaaattg ctacataggg gccttctgct
     7681 ttaccgccac cattataaat accttgatct acagcatgtg accatttact tttcgctggc
     7741 aattgttctg gtgtatacac aatacctttt gctttctctg gattagtaat tgctaatgtc
     7801 gatccggcat gcatagagac agatttcaca ccttcagtaa taccgaaact ttcttttgaa
     7861 gaaacaatat tgctcgtatt taaatcacct agtgcattat atcgaaaacg tacgccaaag
     7921 tttgtagata accaatctga acttttcaca ccttgcattg cagtagagct tttttcttct
     7981 gcattcatac ctttcgacat atcttcatat gctccacgtc gataaccatt cattgcctcc
     8041 gatgaatcaa tacgatttaa atttcggtca gcattgtaat gatctgaaat aaagacaaca
     8101 ttgccacctt gtttcacata gttaacaatt gctgcctgtt ctgattcttt gaaaggaatg
     8161 ttagcctcag gaattacaaa tattttggaa cttttcaaac ttgcttctgt tatgttcgaa
     8221 tgaccatcaa tagctttaac gtcataacct tgtttttgta ttgaatccgc ataatctgaa
     8281 aatgcaccat cactaaccca atctgcagca ccagctgttt gaccatgaga acgatcgaat
     8341 aataccgttc gctgttgctt tgtaggttgc gattcatgcg ttatagctaa agattgcggt
     8401 aaagcactta atgataccgt tgcaacaatt gcagagacag ttaatgactt atatattttt
     8461 ttcattttgt gaggctcctt ttaaaataaa tttgttcttg aattatagga taaaaattcg
     8521 ttgcatatga gcaatttaac gaaaagttta caaaatctta tcaaactctt aaagaaagtt
     8581 attaaaattc atttttataa aatacttttt cacatttaaa tgtggtacgc tataagtgta
     8641 atttcattgc atacatatta cacgattaag aatgtgaagg ggacagttat caaatgaaaa
     8701 attttaagta tttatttgta ttaatgttag cagtcattgt ttttgcagca gcatgtggaa
     8761 actcaagttc tttagataat caaaagaacg ctagtaatga ttcggattct aaatcaggag
     8821 gatacaaacc taaagaatta acagtacaat ttgtaccttc gcaaaatgct ggaacattag
     8881 aggctaaagc aaaaccatta gaaaaattac tatctaaaga attaggaatt ccagttaaag
     8941 tgtctgtatc aactaactac aatacaattg ttgaagctat gaagtctaaa aaagttgatg
     9001 ttggtttctt accaccaacg gcatacacat tagcacatga tcaaaaagca gctgatttat
     9061 tattacaagc acaacgtttt ggtgtaaaag aagatggttc agcaagtaaa gaacttgtag
     9121 atagttataa atcagaaatt cttgttaaaa aagactcaaa aattaaaagc ttgaaagatt
     9181 taaaaggtaa gaaaattgcc ttacaagatg taacatcaac tgctggatat acattcccac
     9241 ttgcgatgtt aaaaaacgaa gcaggtatta atgcaactaa agatatgaaa attgtgaatg
     9301 ttaaaggtca tgaccaagca gttatctcat tattaaatgg agatgtagat gctgcggctg
     9361 tatttaacga tgcacgtaat actgtgaaaa aagaccaacc aaatgtattt aaagacacac
     9421 gaattttaaa attaacacaa gctattccga atgacacaat ttctgtaaga ccagatatgg
     9481 ataaagattt ccaagaaaaa ttgaaaaaag cttttataga cattgctaaa tcaaaagaag
     9541 gtcacaaaat tattagcgaa gtttattcac atgaaggata cacagaaacg aaagattcaa
     9601 atttcgacat tgtaagagag tacgaaaaat tagttaaaga tatgaaataa tcattatcga
     9661 atttggtatt aaaagctttc gttcaataga tatattctag ataaatattg aaaagctagg
     9721 cgctaaactg aaacagatat agaaaggtgt cgctgtacat ttgaaaccat ttgtacacag
     9781 aaacccaatg tctatgatat ttcagtttac cttggctttt ctttattaaa gaaaggtgtc
     9841 aaacatgagt caaatcgaat ttaaaaacgt cagtaaagtc tatcctaacg gtcatgtagg
     9901 cttgaaaaat attaacttaa atattgaaaa aggtgaattt gcagttattg tcggactatc
     9961 tggtgctggg aaatccacgt tattaagatc tgtaaatcgt ttgcatgata tcacgtcagg
    10021 tgaaattttc atccaaggca aatccatcac taaagcccat ggtaaagcat tattagaaat
    10081 gcgccgaaat ataggtatga ttttccaaca ttttaattta gttaaacggt caagtgtatt
    10141 acgaaatgta ctaagtggac gtgtaggtta tcaccctact tggaaaatgg tattaggttt
    10201 attcccaaaa gaagacaaaa ttaaagcaat ggatgcacta gaacgcgtca atatcttaga
    10261 taaatataat caacgctctg atgaattatc aggtggccaa caacaacgta tatctattgc
    10321 acgtgcgcta tgccaagaat ccgaaattat tcttgcagat gaaccagttg cttcattaga
    10381 cccattaaca acgaaacagg ttatggatga tttaagaaaa atcaaccaag aattaggcat
    10441 cacaatttta attaatttac attttgttga cttggcaaaa gaatatggca cacgcatcat
    10501 tggtttacgt gatggtgaag ttgtctatga tggtcctgca tctgaagcaa cagatgacgt
    10561 atttagtgaa atatatggac gtacaattaa agaagatgaa aagctaggag tgaactaaca
    10621 tgcctttaga aatacctaca aagtatgact cccttttaaa gaaaaaggtt tctttaaaaa
    10681 cgagttttac cttcatgtta atcattgtgc ttatcatttg gagcttcatt tatacggggt
    10741 ttaattttgg agatttaatg ataggaatac ctcaaatagg tgatctattc aaacaaatga
    10801 ttccacctga tttcgagtat ttacaacaaa ttacaacgcc aatgttagat accattcgaa
    10861 tggctatcgt aagtacagta ttaggtagca tcgtttcaat accaattgcg ttattatgtg
    10921 ctagcaatat cgttcatcaa aagtggattt caataccctc gcgctttatt ttaaatatag
    10981 ttcgtactat tccagatttg ttattagcag caatctttgt ggctgtattt ggaatcggtc
    11041 aaattccagg gatattagca ttgtttattt taactatctg tattattgga aaattattat
    11101 atgaatcatt ggaaacgata gatccaggtc caatggaagc aatgacggct gttggcgcta
    11161 ataaaataaa atggattgtt ttcggtgttg taccacaagc catatcgtca tttatgtcat
    11221 acgtattata tgcatttgaa gtaaatatac gagcttcagc tgtgcttgga ttagtcggcg
    11281 ctggcggtat tggattgttt tatgatcaaa cacttggttt atttcaatat ccaaaaacag
    11341 caacgattat tttatttact ttagttatcg tcgtcgtcat tgattacatc agtacgaaag
    11401 tgagggcaca tctcgcatga cacaggaaat agcaaaatat aatgttcaca caaaagcaca
    11461 caaacgaaaa ttgattaaaa gatggcttat tgcaattgtc gtcttagcta ttatcatctg
    11521 ggcatttgca ggtgtaccaa gtttagaact taaaagtaaa tcattagaaa tcttaaaatc
    11581 catattcagc ggattattcc atcctgacat cagctatatc tatataccag atggcgaaga
    11641 cttattacgt ggtttacttg aaacctttgc gatagccgtt gtaggtactt tcatcgccgc
    11701 aattatctgt attccattag catttttagg tgcaaataat atggtaaagt tacgcccggt
    11761 ttcaggtgtt agcaaattta ttttaagtgt tatacgtgtc ttcccagaaa ttgtaatggc
    11821 acttatattt atcaaagctg ttggcccagg ttcattttca ggtgtattag ctttaggtat
    11881 ccattctgta ggtatgcttg ggaaactttt agctgaagat attgaaggtc tagatttcag
    11941 tgctgtagaa tcattaaagg ccagtggtgc gaataagatt aaaacactcg tatttgcagt
    12001 cataccacaa attatgcctg cctttctatc actcatactt tatcgctttg aactaaactt
    12061 acgttcagct tctatactgg ggctaattgg ggctggtggt atcgggacac cactcatatt
    12121 tgccattcaa acacgttctt gggaccgtgt aggtattata ttaatcggtt tagtactaat
    12181 ggtcgcaatt gtcgatttaa tttccggttc aatccgaaaa cgtattgttt aacattaaat
    12241 caggatactc ctaaataaga ggtcctaccg tcacgtttct ctattataat aaaaacagca
    12301 gtgaagaaaa ctattgttat agttaacttc actgctgttt ttataatatc taaatttatt
    12361 ctatttcaat tcctttaaat aacttttacc gaactctggt aatgttacgt tgaaattatc
    12421 tgctatagtt gcaccgatag aactgaatgt agtatcactt tctagtgcat gaccaccttt
    12481 aaatttcgga ctgtacataa ttactgggat atattctctc gtatggtccg tacctggcgc
    12541 tgtcgggtca ttaccatggt ctgctgtaat aattactaaa tcgtcttctt ttaagttgct
    12601 aaacagttct ggcaagcgat catcgaaatc tttaattgct tgtgcataac ctggtttatc
    12661 acgacgatga ccgtataatg catcaaagtc tactaagttt aagaagctaa tacctgtgaa
    12721 atctttctta acaattttca tcaattgatc cataccgtcc atgttactct tcgtacgaac
    12781 cgcttctgtt acaccttcac catcataaat gtcattaatt ttaccgatgg caataacatc
    12841 ataaccaccg tctttcaaat gatctaagac agttttacca aatggtttta acgcatagtc
    12901 atgtcgatta gatgtacgtg taaagtttcc tggttcacca acatatggac gtgcgataat
    12961 acgaccaatt aaatatttag ggtcttttgt caactcacga accttttcac aaatatcata
    13021 taactcttct aatgggataa tgtcttcatg tgcagcaatt tgcaatactg ggtctgcact
    13081 tgtataaaca attaagtcac cagttttcat ttggtgctcg ccccactcat cgataatttg
    13141 cgtacccgat gccggtttgt tagcaacaac tttacgacct gtcatttctt caatttgttg
    13201 aattaactct tcagggaatc cattagggta tactttaaaa ggttgcataa tatttaatcc
    13261 cataatttcc cagtgaccag tcattgtatc tttaccaact gaagcttcac tcaatttagt
    13321 atagtatgct tctggttgtt caactgcatt tactactggt aatttatcga tgttccctag
    13381 acctaacttt tcaaggtttg gtaaagtttg atcgaaacct tctaaggtat gtcttaaagt
    13441 atgtgaacct tcatctttaa aatcagctgc gtctggcgct tcaccaatac ctactgaatc
    13501 cattacgatt aaatgtacac gattaaatgg tcttgtcata gctatcactc ccaaatttta
    13561 tata
//
LOCUS       DAOBBH010000058        13546 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.058, whole genome shotgun sequence.
ACCESSION   DAOBBH010000058 DAOBBH010000000
VERSION     DAOBBH010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13546
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.058"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(39..368)
                     /locus_tag="P6L01_001232"
     CDS             complement(39..368)
                     /locus_tag="P6L01_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030309.1"
                     /translation="MINETKYMRQQITNLIQIIDTIKYNTLMTDWNIQTHIYKFNQIV
                     TNELNKFKGFETSYIINENQVSYYEIITLLNKRPLRQVDYGNKIQYLNFYHTELSNAL
                     FAIKFAH"
     gene            417..1154
                     /gene="sdpB"
                     /locus_tag="P6L01_001233"
     CDS             417..1154
                     /gene="sdpB"
                     /locus_tag="P6L01_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpB"
                     /protein_id="HDP3030310.1"
                     /translation="MENEKKKYTFKDIAWRDLLIIPIIGVIFYALTIAAYIGPILFGY
                     YEIDSNLIFVATITQALAYTIGIGAFYLFHLKVMPSRLRAGFAYIKKYWLRLSITYII
                     ALILIYTYEYMTQFLPKHLQYSETANELELNKMFEVPAFLPVAFLLIVIVGPIVEEIV
                     FRHILIGELGKKFNFIAMSIVSVFLFAFIHVTDAKSPFEFGPYLILSIILVFTYLKSG
                     RNLGSTIALHIANNFVSFVISVISIYA"
     gene            complement(1179..1871)
                     /locus_tag="P6L01_001234"
     CDS             complement(1179..1871)
                     /locus_tag="P6L01_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDP3030311.1"
                     /translation="MNKAERQNLIITAIQQNKKMTALELAKYCNVSKRTILRDIDDLE
                     NQGVKIYAHYGKNGGYQIQQAQSKIALNLSETQLSALFLVLNESQSYSTLPYKSEINA
                     IIKQCLSLPQTRLRKLLKRMDFYIKFDDTQHMTLPMLFSDILIYCTERNVMLVDHRVD
                     DNIKAENVIFIGLLCKHGHWHAVIYDIAQDKTAELEIENIIDISYSFGKTIQTRDISI
                     DNYHQFLNPIDS"
     gene            2256..3053
                     /locus_tag="P6L01_001235"
     CDS             2256..3053
                     /locus_tag="P6L01_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HDP3030312.1"
                     /translation="MTDKTLQQIDKLICSWLKQIDNVIPQLIMEMTTETKRHRFDLVT
                     NVDKQIQQQFQQFLATHFPEHQLLAEEKSNEMITNEINHLWIMDPIDGTANLVKQQED
                     YCIILAYFYEGKPMLSYVYDYPHKKLYKAIRGEGAFCNGIKMEEPPSLKLEDAIISFN
                     AQVMNLDTVQDLFDASFSYRLVGACGLDSMRVAKGQFGAHINTNPKPWDIAAQFLFAE
                     LLNLKMTTLDGKAIDHLKGAPFIISNKACHETVLKILNANGGYQKYR"
     gene            3186..4133
                     /locus_tag="P6L01_001236"
     CDS             3186..4133
                     /locus_tag="P6L01_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDP3030313.1"
                     /translation="MKRSSKSKMSLPKKIFLWVFGILVILAIVAVVYVAAKIFITGNK
                     IHNPLDRNHSELRDKKVSLNDGDPFTIALFGVDSDADRKKKGGGERSDSIMILSINPK
                     TKKTEIVSIPRDTRAEIVGRGTTEKIAHAYAYGGPNMAVKSLEKLMNVPIDHYATIDM
                     DGLHNMIDSIGGVDVVSNDTFTVDGVRFTKGQQTHVNGDQALKFIRSRKEEGAGGDFG
                     RQQRQQIVLEAMANKIASPSSITHFNSLMNEIQNNVKTDLTLGDLNTIRSNYKDANDT
                     INKHQLSGQGGIQSDGLYYFIPSEQSKAESTKLLKDNLE"
     gene            4736..7690
                     /gene="fdhF"
                     /locus_tag="P6L01_001237"
     CDS             4736..7690
                     /gene="fdhF"
                     /locus_tag="P6L01_001237"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDP3030314.1"
                     /translation="MQEHLVVTLDGKDYLVEPGTNLLEFIKSQDTFVPSICYNESMGP
                     IQTCDTCTVEIDGKIERSCSTVIDRPMTVNTVNNDVKDAQKEALDRILEKHMLYCTVC
                     DYNNGDCEIHNTMDAWGHQHQTYEYKEKPYEKDYGPFYRYDPNQCILCGRCVEACQDI
                     EVNETIRIDWDREHPRVIWDNDVPINESSCVSCGQCATVCPCNAMMEVNMEGNAGYMT
                     DTEPGSLAAMIDLTKKAEPGYGPLFAISDSEAEMRKERIKKTKTVCTYCGVGCSFEVW
                     TKDREILKVQPSHDSPANKIATCVKGKFSWGHINSDQRLTKPLVRKNGEFHEVEWDEA
                     LNVIADNFTSIKEKYGPDALSFISSSKATNEESYLMQKLARQVIGTNNVDNCSRYCQA
                     PATKGLFRTVGHGGDSGSIEDLEKAAMSVLIGTNTAEAHPVIASRMKRAQKLFGQKIH
                     VFDIRKHEMAERADRFYQPKPGTDLAWLSAVTKYIIDHDLHDKAFIDEWVDDFDEYYK
                     SLETFTMAFAEEATGIPESELIKFAEECAKAESVVICWAMGITQQDIGSDSSTAISNL
                     LLVTGNYRRPGTGAYPLRGHNNVQGCSDMGSMPDKITGYQSIEADDIRAKFEKEYGVK
                     LNPKAGKDNHEMVEGIHDGEVHSLYLYGEDTGIVDSNINFVQAAFEKLDFMVVQDEFL
                     TFTATYADVVLPASPSLEKDGTFTNTERRIQRLYQALEPLGDSKPDWKIFQAIANRLG
                     FDWNYKHPSEIMDEVARLTPLYAGVSYDRLEGFNSLQWPVQPDGTDEPILYLEGFNFD
                     NGKAKLFPLSFDNYFKQDEIYDIHVNNGRLLEHFHEGNMTYQTPMIKYKVPRAFVEIS
                     PELAEDRGIHEGAEVKLISETGEAVLQVHVTDRVKGKEIYIPLNNDAMENGDLGAINL
                     LTNSDVDQYTDTPSYKRTSCRLEVITKRGKSPLNPNNFRVNKKRHPQYSVQVQKKWER
                     SDYVFPGNQVDK"
     gene            7690..8163
                     /locus_tag="P6L01_001238"
     CDS             7690..8163
                     /locus_tag="P6L01_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HDP3030315.1"
                     /translation="MAERISSKIRRLEKSEEQIKLESLNEVTEAIAANKDSILKAIKL
                     IKTLDDAKLLDALNGAIRGRQVIINKFAVELNKDIYTGLLSNMASMVFLLGELNVSDL
                     SDFLNKVNKGLHVANQASPNAKTTIRSLLGVLKDDDMNRSLTYMLNMLKGMSREE"
     gene            8391..8624
                     /locus_tag="P6L01_001239"
     CDS             8391..8624
                     /locus_tag="P6L01_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030316.1"
                     /translation="MLKTSAVISNTLLVIGLVCLFMMKLVLAITFFAVSLSISLVVFN
                     MMFRDRTTMKVIVNASFLIVILAIVVAYFLLSK"
     gene            8646..9422
                     /locus_tag="P6L01_001240"
     CDS             8646..9422
                     /locus_tag="P6L01_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDP3030317.1"
                     /translation="MKKNFKLRISTLLLIVILVVFAVLLIVNETKLFKNDVNYSFDEA
                     VSMQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQ
                     LLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNF
                     FGIGAFDSSAVRSGKSYAEKEQWTSPDKAIIGGAKFIRNEYFENNQLNLYQMRWNPEN
                     PAQHQYASDIRWADKIAKLMDKSYKQFGIKKDDIRQTYYK"
     gene            9733..10857
                     /locus_tag="P6L01_001241"
     CDS             9733..10857
                     /locus_tag="P6L01_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDP3030318.1"
                     /translation="MKIAIIGAGIGGLTAAALLQEQGHTIKVFEKNESVKEIGAGIGI
                     GDNVLKKLGNHDLAKGIKNAGQILSTMTVLDDKDRLLTTVKLKSNTLNVTLPRQTLID
                     IIKSYVKDDVIFTNHEVTHIDNETDKVTIHFAEQESEAFDLCIGADGIHSKVRQSVNA
                     DSKVLYQGYTCFRGLIDDIDLKHPDCAKEYWGRKGRVGIVPLLNNQAYWFITINSKEN
                     NHKYSSFGKPHLQAYFNHYPNEVREILDKQSETGILLHNIYDLKPLKSFVYGRTILLG
                     DAAHATTPNMGQGAGQAMEDAIVLVNCFNAYDFEKALQRYDKIRVKHTAKVIKRSRKI
                     GKIAQYRSRLFVAVRNRIMKMMPNALAAGQTKFLYKSKEK"
     gene            complement(10949..11902)
                     /locus_tag="P6L01_001242"
     CDS             complement(10949..11902)
                     /locus_tag="P6L01_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HDP3030319.1"
                     /translation="MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKN
                     ATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNA
                     TADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
                     ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLID
                     TEQFKMMKSTAYLINASRGPIVHEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLN
                     NVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN"
     gene            complement(12263..12763)
                     /locus_tag="P6L01_001243"
     CDS             complement(12263..12763)
                     /locus_tag="P6L01_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDP3030320.1"
                     /translation="MKKLVTATTLTAGIGTALVGQAHHADAAENYTNYNNYNYNTTQT
                     TTTTTTTTTTSSISHSGNLYTAGQCTWYVYDKVGGEIGSTWGNANNWAAAAQGAGFTV
                     NHTPSKGAILQSSEGPFGHVAYVESVNSDGSVTISEMNYSGGPFSVSSRTISASEAGN
                     YNYIHI"
     gene            complement(13000..13422)
                     /locus_tag="P6L01_001244"
     CDS             complement(13000..13422)
                     /locus_tag="P6L01_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HDP3030321.1"
                     /translation="MTTEMNDVTNHQPNRKSNDENIMAMLIYLLSLFTSIIGPLIIWL
                     LKKDESKLVDRAGKNYLNYTISYIIWSIVLVVITLIGVFLIATDISFIIIIGFIITFI
                     GILSIFAFSILSFVFTIIACVKYYNGQEYVIPLTIRFI"
BASE COUNT         4802 a         1851 c         2504 g         4389 t
ORIGIN      
        1 taaataactt gtttaaatga cattttatga aaaataggtt aatgggcaaa tttaattgca
       61 aataatgcgt tagatagttc tgtatgataa aaattaagat attgaatttt gttaccatag
      121 tcgacttgtc ggaggggacg tttattaagt agtgttataa tttcataata ggaaacttga
      181 ttttcgttta ttatatatga tgtttcaaag cctttgaact tattcaattc attagtaact
      241 atttggttaa atttataaat atgcgtttga atgttccaat ctgtcattaa agtattatat
      301 ttaatcgtat caatgatttg aatcaaatta gtaatttgtt gtctcatata cttagtttcg
      361 tttatcatgt agaagtacct ccaaaaatca attattaaac aggaaaggat ataacagtgg
      421 aaaatgaaaa aaagaaatac acgtttaaag atatagcatg gagagatttg cttataattc
      481 caattatagg tgttatattt tatgcactca caattgcagc ttacatagga ccaattttgt
      541 ttggttatta tgaaatcgat agtaatctga tatttgtagc gactatcaca caagctttag
      601 catatacgat tgggattggc gcattttatt tatttcattt aaaagtgatg ccatcaaggc
      661 ttcgagctgg ttttgcgtat atcaaaaaat attggcttag attgtcaata acgtatatta
      721 ttgctctcat cttaatttat acatatgaat atatgacaca gtttctacct aaacatttgc
      781 aatatagtga aacagccaat gaacttgagc taaataaaat gtttgaagtg cctgcatttt
      841 tacctgtagc attcttatta attgtgattg taggtccaat agttgaagaa atcgtattta
      901 gacatatttt aattggagaa ttaggtaaga agtttaattt tatagctatg agtattgttt
      961 cggtgttctt atttgcattt attcatgtga ctgatgcaaa gtcgccgttt gagtttggtc
     1021 catatctcat actatccatc atattagttt tcacttattt aaaatctgga agaaacctag
     1081 gttcaacaat tgcattacat atagcaaata attttgtttc tttcgttata tcagtaataa
     1141 gtatttatgc gtagataaaa ccccatcgac tccatagttt aggaatcgat ggggtttaaa
     1201 aattgatgat agttatcaat ggatatgtct ctggtttgaa tcgtcttacc gaatgaatac
     1261 gaaatatcta taatattttc aatttcgagt tcggcagttt tgtcttgagc aatgtcataa
     1321 atgactgcat gccaatgtcc atgtttacac aaaaggccaa taaatataac gttttcagct
     1381 ttaatattat catcaaccct atgatctact aacatcacat ttcgttctgt acaataaatt
     1441 aaaatgtcgg aaaacagcat tgggagtgtc atatgttgtg tgtcatcaaa tttaatataa
     1501 aagtccatgc gtttaagcaa ttttcttaag cgtgtttgtg gaagacttaa acattgtttt
     1561 ataattgcgt tgatttcgct tttatatggt aatgtcgagt acgactgact ttcattaagc
     1621 actaaaaata aggccgataa ttgtgtttca gataagttta atgcaatttt agattgtgct
     1681 tgttgtattt ggtaaccacc atttttccca taatgcgcat aaattttaac accttgattt
     1741 tctaaatcat caatatctct taaaattgtg cgtttggata cgttgcaata tttagctaat
     1801 tctaaagcgg tcattttttt attttgttga attgcagtaa ttattaaatt ttgcctttca
     1861 gctttattca tgacagccac ctcctaaaag atgatatatt acacaaagta taacataaga
     1921 gtgacaatga ggacattttc aatatctttt aaaaaaaatc gtaaaaattt tttggaaaac
     1981 gcttacattt taaaaaagat tgtgctatga tattacttgt aacaggggga gcgattaaac
     2041 aaaggggtag agctatataa cagatagctt ttaatcgttc aagttacatc acaacatctt
     2101 caatatttat tacttacttt cctttctatt tgtcggctag cacgtgacta gccgattttt
     2161 ttatgcataa aatgaagaag tcttcagttg tattgttgat tcttttaata tagggtaata
     2221 ataaaatgat aatgatatga gaagtggtgg acgaaatgac agataaaaca ttacagcaaa
     2281 tagataagtt aatatgttcg tggttgaaac aaatagacaa tgttattccg caattaatta
     2341 tggaaatgac tacagaaact aagcgtcatc gatttgattt agttacaaat gtagataaac
     2401 agattcaaca acaattccaa caatttttag caacacattt tccggaacac caactattag
     2461 cggaagaaaa aagtaatgaa atgattacga atgaaattaa tcatttatgg attatggatc
     2521 ccattgatgg aactgccaat ttagtaaaac agcaagaaga ttattgtatt atattggcgt
     2581 atttttatga aggtaaacca atgttatcgt acgtatatga ttatccgcat aaaaagcttt
     2641 ataaagcaat acgaggagaa ggtgcttttt gtaatggaat taagatggaa gaaccaccat
     2701 cgttaaaatt ggaagacgct attatatcgt ttaatgcaca agtgatgaat ctagatacgg
     2761 tgcaagattt atttgacgcc tcatttagtt atcgtttagt tggtgcttgc ggattagatt
     2821 ctatgcgagt ggcaaaaggt caatttgggg cacatattaa tacaaatcct aaaccttggg
     2881 acattgctgc acagttttta tttgcagaac tattgaatct aaaaatgacg actttagatg
     2941 gtaaggcaat tgatcattta aagggtgcac cttttattat aagtaataaa gcatgtcatg
     3001 aaacggtact taaaatttta aatgcaaatg gtggttatca aaaatacaga tagatattct
     3061 ctagagataa aaaatgataa aaaatagtta tacgtctaca gaagtatagt aaaaaaatta
     3121 ttttactata taataaatag agaaaacaga caaagtatgt aaggaatagt aaaggagtgg
     3181 tgggattgaa acgttcgtct aaaagtaaaa tgagcctacc gaaaaaaata tttttatggg
     3241 tattcggtat tttagtaatc ttagcgattg tagctgttgt gtatgtagct gctaaaattt
     3301 ttattactgg taataagata cacaatccgt tagatcgcaa tcattcagaa ttaagagata
     3361 aaaaagtgag tttaaacgat ggtgatccat tcacaatcgc tttatttggt gtcgattcag
     3421 atgctgatcg taagaaaaag ggtggcggag agcgtagtga tagtattatg attttatcta
     3481 tcaaccctaa aacgaagaaa actgaaatag taagtatacc gcgtgataca agagcggaaa
     3541 ttgttggccg tggtactact gaaaaaattg cacatgccta tgcatatggt ggaccaaata
     3601 tggctgttaa atcacttgaa aaattaatga atgtgccaat tgatcattat gcgacaatcg
     3661 atatggatgg tttgcataat atgatagata gcatcggcgg tgttgatgta gtaagtaatg
     3721 atacattcac tgttgatggt gttcgtttta caaaaggaca gcagacacat gtaaatggtg
     3781 atcaagcatt gaagtttatt agaagtcgta aagaagaagg tgctggtggt gactttggtc
     3841 gacaacaacg tcagcaaatt gtgcttgaag cgatggccaa taagattgca agcccatcat
     3901 cgattacaca ttttaatagt ctaatgaatg aaattcaaaa taatgttaaa acagatttaa
     3961 cattaggtga tttgaatacg attagaagca attataaaga tgctaatgac acgattaata
     4021 aacatcaatt gagtggtcaa ggcggcattc aaagtgacgg attgtactac ttcattccta
     4081 gtgagcagtc aaaagctgaa agtactaagt tattaaaaga taatctagag tagtaaacta
     4141 agggaaaata agttatcaaa attgtacaca aattaattag atagaatgat ctgtaaatag
     4201 aataacgaat agaactaata gcccgtaaat agaaggtgat aacactttgt atttatgggc
     4261 tattttattt aatgcctgat ttgattttta taaattaatc cattacaaat ttaatttaaa
     4321 gataaatatt accttctaaa tggtagtaga tatttatcaa acttgggtta ttaattttca
     4381 gaaaaagggt atttataaat agtataacga tttttaggtg aggtgattat taagtattgt
     4441 cattaataag gttgtgaaag atatgactga aaagcgtgaa acagataatt ggaaaactaa
     4501 taatgataat tattttcaat taaacaaaaa gatgatttaa tttcgcgcaa aatatttcaa
     4561 ataaaaatga ttgtaaaaag gcaaaataca aaatttcact taacaactag tacataaagt
     4621 aatacaatta aattaattct atctgaaaga tgtgtggggc atcgttattt taggtggata
     4681 tgagcaattt attaaaagtc atttacggaa aatatatata gacggggtga gtaatatgca
     4741 agaacatttg gtggttacac ttgatggaaa agattatctt gtagaaccag gtacgaattt
     4801 acttgaattt attaaatcac aagatacttt tgtaccttca atttgttata acgagtcgat
     4861 gggaccaatt caaacatgtg atacatgtac tgttgagatt gacggtaaaa ttgaacgttc
     4921 atgtagtacg gtgattgatc gtccaatgac tgtaaatact gtgaacaatg atgtgaaaga
     4981 tgctcaaaaa gaagcgcttg atcgaatttt agaaaagcat atgctgtatt gtacagtatg
     5041 tgattataat aatggtgatt gtgaaattca taatacgatg gatgcatggg ggcatcagca
     5101 tcaaacgtat gaatataaag agaagccata tgaaaaggat tatggcccat tttatcgtta
     5161 tgatccaaat caatgtatct tatgtgggcg ttgtgtagaa gcatgtcaag atatcgaagt
     5221 gaatgaaact attagaattg attgggatcg tgaacatcca cgtgttattt gggataatga
     5281 tgtaccaatt aatgaatcgt catgtgtatc ttgtggtcaa tgcgcaactg tatgtccatg
     5341 taatgccatg atggaagtga atatggaagg taatgcgggt tatatgactg atacggaacc
     5401 tggttcatta gcagcaatga ttgatttaac taaaaaagca gaacctggtt atggtccact
     5461 atttgcaatc tcagattcag aagcagaaat gcgtaaagag cgtattaaga aaactaaaac
     5521 agtgtgtact tattgtggtg tgggttgttc atttgaagtt tggactaaag atagagaaat
     5581 tttaaaagta caaccatcac atgattctcc agcaaataaa attgcgactt gtgttaaagg
     5641 taagttttca tggggacata ttaattcaga tcaacgctta actaaaccac tagtaaggaa
     5701 aaatggtgag ttccatgaag tagaatggga tgaagcttta aatgtcattg cagataattt
     5761 tacatctatt aaagaaaagt atggcccaga tgcactatca ttcatttctt cttctaaagc
     5821 gacgaatgaa gaatcgtatt taatgcaaaa attagcaaga caagttattg gcacaaataa
     5881 cgttgataac tgttcaagat attgccaagc acctgcaaca aaaggcttat ttagaacggt
     5941 tggacacggc ggtgactcag gtagtattga agatttagaa aaagcggcaa tgtctgtatt
     6001 gataggtacc aatacagccg aagctcatcc agttatcgca tcacgcatga aacgtgctca
     6061 aaaattattt ggacaaaaaa tacatgtatt tgatattaga aaacatgaaa tggcagaacg
     6121 tgcggatcgt ttttatcaac ctaaaccagg tacggattta gcgtggttaa gtgcagtgac
     6181 taagtatatt attgatcatg atttacacga taaagcattt attgatgagt gggtagatga
     6241 ttttgatgaa tattacaaat cattagaaac atttacaatg gcttttgctg aagaagcaac
     6301 aggtattcct gaatcagaat tgattaaatt tgctgaagaa tgtgctaaag ctgaatctgt
     6361 tgtaatttgt tgggcaatgg gtattacaca acaagacatt ggtagtgact caagtacagc
     6421 gatttcaaac ttattattag taacaggtaa ttatcgtcgt cctggtactg gtgcgtatcc
     6481 attacgtgga cataataatg ttcaaggatg tagtgatatg ggaagtatgc ctgataagat
     6541 tactggttat caaagtattg aagcggatga tattcgcgct aaatttgaaa aagaatatgg
     6601 cgttaaattg aatccaaaag ctggtaaaga taatcatgaa atggtagaag gtatacatga
     6661 cggagaagta cactcattgt acttatatgg tgaagatacg ggtattgtgg attcaaatat
     6721 taattttgta caagctgcgt ttgaaaaatt agatttcatg gtagtccaag atgaattttt
     6781 aacattcaca gcaacatacg cagatgttgt attgccagca agtccttcac ttgaaaaaga
     6841 cggtactttt acaaataccg aacgtcgtat tcaacgttta taccaagcat tagaacctct
     6901 tggtgattca aaacctgact ggaaaatttt ccaagcaatt gctaatagat taggatttga
     6961 ttggaattac aagcatccta gtgaaattat ggatgaagta gcacgcttaa cacctctata
     7021 tgctggggta agttatgatc gtttagaagg attcaatagt ttacaatggc cagtacaacc
     7081 tgatggtact gatgagccta ttctatactt agaaggattc aattttgata atggtaaagc
     7141 aaaattattc ccattatcat ttgataatta ctttaagcaa gatgaaattt atgatattca
     7201 tgtaaacaat ggtcgattgt tagagcactt ccatgaaggt aatatgactt atcaaacacc
     7261 aatgattaaa tacaaagtgc cacgtgcatt tgttgaaatt tctccagagc ttgctgaaga
     7321 tagaggcatt catgaaggtg cagaagttaa gctgatttct gaaacaggag aagcggtatt
     7381 acaagttcac gtgacagatc gtgttaaagg taaagaaatc tatattccat taaataatga
     7441 tgcgatggaa aatggagatt taggtgcaat taacttatta actaatagtg atgttgatca
     7501 atacacggat acaccatctt ataaacgtac tagctgtcgt ttggaggtaa ttacgaaacg
     7561 tggtaagtca ccgttgaacc ctaataattt ccgtgtcaat aaaaaacgtc atccgcagta
     7621 cagtgttcaa gtacagaaaa aatgggaacg ttcagattat gttttcccgg gaaatcaggt
     7681 ggataaataa tggctgagag aatatcttct aaaattagac gcttagaaaa atcagaagaa
     7741 caaattaagt tagaaagttt aaatgaagtt acagaggcaa ttgcagcgaa taaagatagt
     7801 atattaaaag caatcaagct tattaaaaca ttagatgatg caaaactatt agatgcttta
     7861 aatggtgcaa ttcgaggacg tcaagtcatc ataaataaat ttgcagttga acttaataaa
     7921 gatatttata cagggctatt atctaatatg gcttcaatgg tatttttatt aggtgaactc
     7981 aatgtatcag atttaagcga cttcctaaat aaagttaata aaggattaca cgttgccaat
     8041 caagcgagtc caaatgctaa gacaactata agaagtctat tgggcgtatt gaaagatgac
     8101 gacatgaacc gtagtttaac atacatgctt aatatgctaa aaggtatgtc tcgagaagaa
     8161 taacattaca atgaaaaagt gtttctacat gcaagcagtg taggagcact ttttttaatt
     8221 tttgtaaaaa ttaaaaaggg tgtttttgat ggaataaatg aataatattg aaaaacaaaa
     8281 aaatcacgtg ctattgtgaa tcaatcatcg acaggcctat gaaacatcat gtattattat
     8341 gattatgtaa gttatttaaa taaaaagggt gagtgcatat taattcgaga atgttaaaaa
     8401 cgtcagctgt aatttctaat acattattag tcataggttt agtctgtcta tttatgatga
     8461 aattagtgtt agctattact ttttttgctg tttcattatc aattagtcta gttgtcttta
     8521 atatgatgtt cagagataga acaacgatga aagtaatcgt taacgcttct tttttaatcg
     8581 taatattagc gattgtagtg gcatattttt tattgtctaa gtaagaaaaa tgaggttttg
     8641 tctatatgaa gaagaatttc aagttacgca tttcaacgct actattgata gttattttag
     8701 ttgtttttgc tgtattactc atcgtgaatg aaactaaatt gtttaaaaat gatgtgaatt
     8761 actcttttga tgaggctgtt tcaatgcaac aagggaaagg tattgtacag acaaaagaag
     8821 aggatggtaa atttgttgaa gcaaataata atgaaattgc taaagcaatg actatttcac
     8881 ataaagacaa tgatatgaag tatatggata tcacagaaaa agtgccaatg tcggaatctg
     8941 aagttaacca attgctaaaa ggtaagggga ttttagaaaa tcgagggaaa gtttttctag
     9001 aagctcaaga aaaatatgag gttaatgtca tttatcttgt tagccatgca ttagtagaaa
     9061 caggtaacgg caaatcagaa ttagcaaaag gcattaaaga tgggaaaaaa cgctattaca
     9121 acttttttgg tataggagca ttcgatagta gtgctgttcg tagtgggaaa agttatgctg
     9181 aaaaggaaca atggacatca ccagataagg cgattattgg tggtgcaaag ttcattcgta
     9241 atgaatattt tgaaaacaat caactgaatt tatatcaaat gcgatggaat ccagaaaatc
     9301 ctgcgcaaca tcaatatgcg agtgacattc gatgggcaga taaaattgcc aaattaatgg
     9361 ataaatccta taagcagttt ggtataaaga aagatgatat tagacaaaca tattataaat
     9421 aagacatcgg tgcttaaagg agctggaaca atttattgtt tcgagctcct ttagcgcatt
     9481 ctgagtgtgt tagttaaatg gattttaacc taacaaaaaa cgctatatag catcaaatat
     9541 gctatatccc acatcattgt tacaaatgta catgatgtaa atgaatattg ctgtctaaat
     9601 gtgcatgtaa tgtacaatgg tgcagataat acacttaagt ccttaaaaat gaaacgttag
     9661 ttccaagagt catttttaaa caatagtgca tgtgataaaa tagaaaagaa tgaaaaatat
     9721 agaggtgaca atatgaagat agcaattata ggtgcaggca tcggtggatt aacagctgct
     9781 gcattattac aagaacaagg tcatactatt aaagtctttg aaaaaaatga gtcagttaaa
     9841 gaaattggcg ctgggattgg tatcggagat aatgtgctta aaaaactagg taatcatgac
     9901 ttagctaaag gtattaaaaa tgctgggcaa atcttatcta caatgacagt gttagatgac
     9961 aaagatcgcc tgttaactac tgttaaatta aaaagtaata cattgaatgt gacgttacca
    10021 cgccaaacat taattgacat tattaaatct tatgtaaaag atgatgtaat atttacaaat
    10081 catgaagtca cgcatataga taatgagaca gataaagtta ccatacattt cgcggaacaa
    10141 gagagtgaag catttgattt atgtattggt gctgatggaa ttcattctaa agtgagacaa
    10201 tctgtaaatg ctgacagtaa agtattatat caagggtata catgctttag aggtttaatt
    10261 gatgatattg atttaaagca tccggattgt gcaaaagaat actggggaag aaaaggaaga
    10321 gtaggtattg ttccgttatt aaataatcaa gcatattggt tcattacaat taactcgaag
    10381 gaaaacaatc ataaatatag ttcgtttggt aaacctcatt tgcaagcata ctttaatcac
    10441 tatccaaatg aagttagaga gatcttggac aaacaaagtg aaacaggtat cttattgcat
    10501 aatatttatg atttgaaacc actcaaatct tttgtttatg gtcgtactat tttactagga
    10561 gatgcagcac atgcgacaac gcctaatatg gggcaaggtg ctggacaagc aatggaagat
    10621 gctatcgtat tagtaaattg ttttaacgca tacgactttg aaaaagcatt acagcgttat
    10681 gataaaatac gtgtcaaaca tactgcaaaa gtaattaagc gttctagaaa aatcggtaaa
    10741 attgcccaat atcgtagtcg tttatttgtt gcagttagaa atcgtattat gaaaatgatg
    10801 ccaaatgcat tagcagctgg acaaactaaa ttcttatata aatcgaaaga gaaataatac
    10861 aacaatatga aaacccccgt atgttgaaac gagagctcaa catatggggg ttcttgtttt
    10921 tataatgtta ttataataaa ttcaattatt agttaacgac aaattgtggt ttctcacctt
    10981 gaacggcact aattgcagca ttagcaacaa ttttagacat catgtcacgt gcttcaaatg
    11041 tagcattacc aatatgcggt gttaatacta cattattaag tgattttaag tcatcggtaa
    11101 tatctggttc aaattcatat acatcaagtg cagcaccttc aatttcatta tctttcaatg
    11161 cttgcactag tgcttgttcg tgcacgattg gaccacgaga ggcattgatt aaatacgccg
    11221 tagatttcat cattttaaat tgttctgtat caattaaatg atgcatttta ggattataag
    11281 cagcgttgat agtgataaaa tctgcattct ttaatagtgt atctaaatct acatattttg
    11341 caccgatttc tcgttctttt tcttctttgc gattaggtcc agtgtatagc acatccatgt
    11401 caaatgctct tgcacgacga gctactgcac taccaatttc acctaaaccg ataatgccga
    11461 ttgttttccc agatacttct ctacctctga aaaataaagg tgcccatccg tcaaatccag
    11521 ttgtacgtga taattggtcc ccttcaacaa tacgacgagc tactgcaagt actaatccaa
    11581 ttgttaaatc agcagtcgcg tttgttgatg ctttaggtgt gtttgtaaca tctatacttt
    11641 tttctcgggc atactcgata tcaatattat taaaaccagc gccatagttg gcaatgattt
    11701 ttaagtcttt accagcatcg ataacatctt tatcaacgtt tgtagataat aaactaatta
    11761 aggcagtcgc gtttttaaca cctttaatta aagtgtcttt atcgactaat cctttacctt
    11821 catacatttc aacttcaaaa tgttcttgta aaagttttaa ccctacttct ggtattgcac
    11881 cagcaacata aactttttcc ataaaagatc actcctttta tcttagtata gtagaagatt
    11941 agacagtata caactatgtc atgatgtctt gtgtatcaat gatgtaagcg cgtacttttg
    12001 atggaggtga tataacttag gcactgtaga actatgaatt ttgtaatgtg gaaaaactgg
    12061 atcaattaaa ttagataacg tagttttaaa gttaatagta ttagaaaaaa ttaatattat
    12121 aaatatgggg gaaatataaa taagtaggtg gcaacgaaaa atagcaaaaa aagagcttct
    12181 cctataaagg aaagctcaaa gttttttgat gacatatgta ctagaattaa gtttcaagac
    12241 aatatgtatc atcgtgttta tattaaatat ggatgtagtt gtagttacct gcttcacttg
    12301 cagaaatagt tctagaactt actgagaaag gtccgccact ataattcatt tctgaaattg
    12361 taactgaacc atcactgttt acactttcta catatgcaac gtgaccaaat ggtccttcag
    12421 aagattgtag gatagcgcct ttagaaggtg tatgatttac tgtgaatcca gcaccttgtg
    12481 cagcagcagc ccaattatta gcatttcccc aagtagaacc gatttctccg cctactttat
    12541 catatacata ccaagtacat tgtcctgcag tgtataagtt accagaatgt gaaattgatg
    12601 atgtagttgt cgtagttgtt gtagtcgttg tagtttgagt cgtgttgtag ttatagttgt
    12661 tgtaatttgt ataattttca gcagcatctg catgatgtgc ttgacctact aatgctgtac
    12721 cgattcctgc tgttaacgta gttgctgtta ctaatttttt catgaataaa gtcctccaaa
    12781 gttctatatc tttttttata aataaaacgt agcgactgtt ttattctcac atctcgaatt
    12841 gatgacaata gttactttaa caaaattaat gcttcttgtg gggaatgtta ttgatttgta
    12901 aaagaataaa aaaactttga ctaattttgt aataaaaatt agtcaaagtt acaatgagat
    12961 taacagataa ttaataggaa atatttattt gtaatatgtt taaataaatc gaattgttaa
    13021 aggtattaca tattcttggc cattataata tttgacacac gcaataattg tgaatacaaa
    13081 agataatatt gagaaagcga atatggataa aataccgata aatgtaatga tgaaacctat
    13141 aatgataatg aaactaatat cggtagcaat taggaaaacg cctattaaag tgataacgac
    13201 taaaacgata gaccaaataa tataagaaat cgtatagtta agataatttt ttccagcacg
    13261 atcaactagt ttcgattcat cttttttcaa taaccatatt atcagtggac caataataga
    13321 tgtgaataaa cttaataaat agataagcat cgccataatg ttctcatcat tggatttgcg
    13381 attcggttga tgatttgtta cgtcgttcat ttcagttgtc atattagaca ctcctttgaa
    13441 aattgtaata ttatctttaa ctataacaaa atataatcaa aaataaacat gtttattaaa
    13501 caattattaa aaataaaaat aattggtgga cgtcggcgtt taaata
//
LOCUS       DAOBBH010000059        13449 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.059, whole genome shotgun sequence.
ACCESSION   DAOBBH010000059 DAOBBH010000000
VERSION     DAOBBH010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13449
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.059"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(156..716)
                     /locus_tag="P6L01_001245"
     CDS             complement(156..716)
                     /locus_tag="P6L01_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDP3030322.1"
                     /translation="MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPV
                     IFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRF
                     SAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
                     KHDFIIEQILSRSCDIESVESWKSSL"
     gene            complement(885..2792)
                     /gene="sasF"
                     /locus_tag="P6L01_001246"
     CDS             complement(885..2792)
                     /gene="sasF"
                     /locus_tag="P6L01_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasF"
                     /protein_id="HDP3030323.1"
                     /translation="MAKYRGKPFQLYVKLSCSTMMASSIILTNILPYDAQAASEKDTE
                     ISKEILSKQDLLDKVDKAIRQIEQLKQLSASSKAHYKAQLNEAKTASQIDEIIKRANE
                     LDSKENKSSHTEMNGQSDIDSKLDQLLKDLNEVSSNVDRGQQSGEDDLNAMKNDMSQT
                     ATTKYGEKDDKNDEAMVNKALEDLDHLNQQIHKSKDALKDASKDPAVSTTDSNHEVAK
                     TPNNDGSGHVVLNKFLSNEENQSHSNQLTDKLQGSDKINHAMIEKLAKSNASTQHYTY
                     HKLNTLQSLDQRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKYAT
                     QSILESIFNKDEADKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQ
                     DKSLLISQILQTKLGKAEADKLAKDWTNKGLSNRQIVDQLKKHFASTGDTSSDDILKA
                     ILNNAKDKKQAIETILATRIERQKAKLLADLITKIETDQNKIFNLVKSALNGKADDLL
                     NLQKRLNQTKKDIDYILSPIVNRPSLLDRLNKNGKTTDLNKLANLMNQGSNLLDSIPD
                     IPTPKPEKTLTLGKGNGLLSGLLNADGNVSLPKAGETIKEHWLPISVIVGAMGVLMIW
                     LSRRNKLKNKA"
     gene            complement(3033..4391)
                     /gene="gtfB"
                     /locus_tag="P6L01_001247"
     CDS             complement(3033..4391)
                     /gene="gtfB"
                     /locus_tag="P6L01_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HDP3030324.1"
                     /translation="MIKLFDYFNDHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVIS
                     PYQFFADNHNTENMKPRFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPR
                     IVSRVEWFDDQQRVRFVDYYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVT
                     SDVILDWQGKSYFFPSKLAFVLFFIKQLEITEHHFVINSLALPFSVLYNLPSKGSDVL
                     VWQEHCNGNVPGNMQLMCKGDMKRHCNIIIPDKNEYETMLNIADAKVQSRILQGGYLY
                     NYRSRNRYTKEIVILTNSDQLRNIKVLVETLPDFNFHIAAITEMSDKLMQLDQYANVH
                     LYPSINIDRVNELYQLCDIYLDINEGNEILNAVEQAFDYELLILGYRQTAHHAKVTLS
                     EHLFEHNDEITMESKDQLIQMLESLKDQQQFRDALLAQKAHAHEISREQFEQVFKQAL
                     ES"
     gene            complement(4384..5892)
                     /gene="gtfA"
                     /locus_tag="P6L01_001248"
     CDS             complement(4384..5892)
                     /gene="gtfA"
                     /locus_tag="P6L01_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HDP3030325.1"
                     /translation="MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQ
                     SENIQTLTQNIGFKDQEVIWLYQYFSDISIAPTTYTLDDIERELGHEISFRERNGKIV
                     RLYFNGKSSFVTCYLQNEQKDVVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAK
                     LYMRQFYNEDGSIAYNEYIDGDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILD
                     RASDIGQAVLQHKGDSKVGVVIHADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFIT
                     ATDIQNHMVCRQFEQYQGYRPRVYTIPVGSIDALSYPKLSRKPYAMISASRLANEKHI
                     DWLVKAVIVAKRQVPELTFDIYGEGSEKTRLRKIIDTHRAQDYIRLLGHVKLDEIYND
                     YELFLSASTSEGFGLTLMEAVGSGLGMIGLNVNYGNPTFIRDGENGYLVPFDTDEDSV
                     DDVIAKLAHAIVMYFNNGPQAPHDISYEVAQQFMTQDIILKWETLVQEVLHD"
     gene            complement(5910..8300)
                     /gene="secA2"
                     /locus_tag="P6L01_001249"
     CDS             complement(5910..8300)
                     /gene="secA2"
                     /locus_tag="P6L01_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HDP3030326.1"
                     /translation="MKHKLDVTINELRLKSIRKIVKRINTWSDEVKSYSDDALKQKTI
                     EFKERLASGVDTLDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTG
                     EGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTASLGFVDIVD
                     YEYQKGEKRNIYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIIL
                     DAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSY
                     FNVEDLYSEQAMVLVRNINLALRAQYLFESNVDYFVYNGDIVLIDRITGRMLPGTKLQ
                     AGLHQAIEAKEGMEVSTDKSVMATITFQNLFKLFESFSGMTATGKLGESEFFDLYSKI
                     VVQAPTDKAIQRIDEPDKVFRSVDEKNIAMIHDIVELHETGRPILLITRTAEAAEYFS
                     KVLFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSMTVATSMAGRGTDIKLGEGVEALGG
                     LAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYS
                     LDAQRLSQSNLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNR
                     VLEIDDAENRDFKALAKDVFEMFVNEEKVLTKSRVVEYIYQNLSFQFNKDVACVNFKD
                     KQAVVTFLLEQFEKQLALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKAS
                     VNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKEGMPVIHFP"
     gene            complement(8290..9249)
                     /gene="asp3"
                     /locus_tag="P6L01_001250"
     CDS             complement(8290..9249)
                     /gene="asp3"
                     /locus_tag="P6L01_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HDP3030327.1"
                     /translation="MLKNKTFKVTWNYISQTTFMYGSKVSFSNGEVTFINPLMPSGLP
                     IHEWLMLKQFSKYKSAPSLPILRRGQHYKLHFDFDATPAGSVYFIIIFYNKNGTKLST
                     EIVKSNSITIQYPDEAYAYKIKMMNAASTSLIFRCLTITEMTHQDDLEYKSMRVTKID
                     DNQYGNDRINVIIAEPSDTYPTISNDFLKLFGHVWLVERWMDDDIKENIKQLKDDLQS
                     QDTLTAINLISYGSKSNVFATYVAQHLDCKVYRTSHEDDDLKEWLTEHVPGNNELKDT
                     NVEFYFKEEQDNHLNYMSRLMNPVRFLDYLDVSKLNGGEVNET"
     gene            complement(9227..10795)
                     /gene="asp2"
                     /locus_tag="P6L01_001251"
     CDS             complement(9227..10795)
                     /gene="asp2"
                     /locus_tag="P6L01_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HDP3030328.1"
                     /translation="MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEA
                     IEAIVEAEGRFDFIYIQAPYSETLTNLLQMISEPYNTYVDESFWSVEYEQDENVQKYV
                     VQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKGDVVYQGNSELTLSGEF
                     GKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAVELQYTFRLIQTGADGALIEQIILT
                     DDMLDSPLEIPAKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDS
                     QRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFY
                     IGSSEYEQGIINVIDETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPL
                     VNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIKALNQKFWQTFQKKSLSQTVF
                     AIAYMQHDDYDPHAFQELLPVLTAHQARVMNRSIPGRHNDDSPTIASWFVNFYNIILE
                     DKFGRVQHAEKQNI"
     gene            complement(10785..12338)
                     /gene="asp1"
                     /locus_tag="P6L01_001252"
     CDS             complement(10785..12338)
                     /gene="asp1"
                     /locus_tag="P6L01_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HDP3030329.1"
                     /translation="MKYFIPAWYDDQRWWQDTTVPYYQLQNKTEFDDMISLMGMHLEN
                     DLDYQLIVLNHAPNLRTFLHRYDLYETKYSSVFDEIQGFSHHAPQAINYHHLKWPDDV
                     EFVYTPYLLKCVTSEQTYTNIYFSQEGYSIWFEEFERDQLQRRYIFDDRGYLSAIRYF
                     DDQGEASYQQYLTINGDCVLHEDLKNGRVTVSKRYQHHYQQTEYNNMAQLIEEKFQAM
                     IAQQIHEDDHVIVASDARHNRQIANHIPAKLLSYSFFKNRNETVSDEEYQSIVKNAHL
                     IVDSVQLERDLISHQEKYQRENTMIRITPFETRQSPNISSQLMETFIGVWIDGMSDAD
                     LQQMMQRLVDYIAQEDYYRLILLSRHQNDIPMWLRECITSVNEEYQAKQNADVNVSAL
                     MTPEDQDDIIAVKTIHAEHDVVEALRTLRLVIDMSKEPDLYLQISAISAGIPQINGQQ
                     TDYVSDYDNGRIINTVDELDDALNYYLFYLKNWNYAYAYSLKLIDAYASKNIINQLDE
                     LIEGENDAT"
     gene            complement(12349..>13449)
                     /gene="secY2"
                     /locus_tag="P6L01_001253"
     CDS             complement(12349..>13449)
                     /gene="secY2"
                     /locus_tag="P6L01_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HDP3030330.1"
                     /translation="YNDMQVKHESFFKIAISNMGGDVNTLNIFTLGLGPWLTSMIILM
                     LISYRNMDKYMKQTSLEKHYKERILTLILSVIQSYFVIHEYVSKERVHQDNIYLTILI
                     LVTGTMLLVWLADKNSRYGIAGPMPIVMVSIIKSMMHQKMEYIDASHIVIALLIILVI
                     ITLFILLFIELVEVRIPYIDLMNVSATNMKSYLSWKVNPAGSITLMMSISAFVFLKSG
                     IHFILSMFNKSISDDMPMLTFDSPVGISVYLVIQMLLGYFLSRFLINTKQKSKDFLKS
                     GNYFSGVKPGKDTERYLNYQARRVCWFGSALVTVIIGIPLYFTLFVPHLSTEIYFSVQ
                     LIVLVYISINIAETIRTYLYFDKYKPFLNQYW"
BASE COUNT         4170 a         2611 c         1873 g         4795 t
ORIGIN      
        1 cgaacgcctc gggtcatttt atataaatat attattttat gttcaaatgt tcctcatcat
       61 atccgtttca attgtcatct cacacatttt ataaatatga gcaaatgtac ttattttcaa
      121 acattactgc ctagctttaa ttgacgttat attaactata aactactttt ccatgactct
      181 acggattcaa tgtcacatga gcgtgataaa atttgttcaa taataaagtc atgtttatca
      241 tctgatctat caccaacagc atcttctaaa acagtaatat aatagtcttt atctacactt
      301 tctaatgccg tgctcaatac agctccactc gtagagacac ccgttaatac taaatgatta
      361 atatcatttg cacgtaaata aacttccaag taactacctg taaatgcgct aaagcgtcgc
      421 ttagaaataa tcggctcatc ttctagtggt gctaaatctt caagtattcg tgtagatgca
      481 tctgcttcag taatcgcata tccttgagct ttaattgttg aaaacacttt attactcgag
      541 gagacatcat taaaatgctt atctaacact aaacgtatga aaatgactgg tattcgatgt
      601 tgtcttgctg cttcaattgc tctctgattc gctttaataa tattttttat tctaggtaca
      661 ctactcgcta taccttcttg catatccaaa actaatagcg ccgtttttcg agacatcttc
      721 attctccttt acttctgtag ttctaagtcg ttaaattcat tataacgtta aaatgatgga
      781 caatctattc attgcatttt gcatatactt cacaataatt taagggggaa ataagacgtc
      841 ttatatactt aaaaaaatat atagatgctc ttcccccaat ataattatgc tttatttttc
      901 aacttattgc gtcgtgataa ccaaatcatt agtacaccca ttgcaccaac aattacagat
      961 atcggcaacc aatgttcttt tatcgtttcc cccgctttag gcaaagatac attaccatca
     1021 gcatttaata atccacttaa caatccatta cctttaccaa gtgttaacgt cttttctggc
     1081 tttggtgtgg gtatatctgg aatactgtct aataaatttg atccttgatt cattaaattt
     1141 gctaacttat ttaaatccgt tgttttccca tttttattca atcgatctag taaacttgga
     1201 cgatttacta ttggtgataa aatatagtct atatcttttt tcgtttgatt gagtctcttt
     1261 tgtaaattca ataaatcatc cgctttacca ttcaatgccg atttaactaa attaaaaatt
     1321 ttattttgat ctgtttctat tttagtaatt aaatctgcca gtaattttgc cttttgtctt
     1381 tctatacgtg ttgctaaaat cgtttcaatt gcttgctttt tatctttggc attattcaaa
     1441 attgctttta atatatcatc tgaagacgtg tcgccagttg atgcaaaatg tttcttcaat
     1501 tggtcaacga tttggcgatt tgataatcct ttattcgtcc aatctttagc caatttatct
     1561 gcttcagctt ttcctaattt cgtttgtaag atttgagaaa tcaatagcga cttatcttgt
     1621 gattgatcaa tcaatgacgt taataaatca tcactcgttg tcagagatag ttgatcaata
     1681 tgacgagtaa tttgatctgc aatttgttga tctgttttac catcaacacg tatatctttt
     1741 agaattttat ctgcctcgtc tttattaaat atactttcta aaatgctttg tgtagcatac
     1801 tttttatcat cagtacgtgc aagttcttcc aaaataatat ttcgttgact ttttatacgc
     1861 tctttcgtct tatttacttc gctcattaag tctgattttt gatttttagg aagttgcgta
     1921 tttgcaatac gttgatctaa agattgtaac gtattcagtt tatgatatgt gtaatgttgc
     1981 gttgaggcat tacttttagc caatttttca atcatagcat gattaatttt atcgcttcct
     2041 tgtaatttat cagtgagttg attactatgg ctttgattct cttcatttga aagaaattta
     2101 tttaacacaa catgtccaga accatcatta tttggcgttt tagctacttc atgattacta
     2161 tctgttgtag acactgccgg atctttcgat gcatctttca atgcatcttt cgatttgtgt
     2221 atttgctgat tcaaatggtc taggtcttct aacgccttat ttaccattgc ttcatcattt
     2281 ttatcatctt tttctccata ttttgttgta gccgtttgtg acatatcatt tttcattgca
     2341 ttaagatcgt cctcgccact ttgttgaccc ctatcaacat ttgaagaaac ctcatttaaa
     2401 tctttaagca attgatctaa tttactgtct atatcacttt gaccgttcat ttcagtgtga
     2461 gaacttttat tttctttgct atccaactca ttagctcgtt ttatgatttc atctatttgc
     2521 gatgctgttt tcgcttcatt tagttgtgct ttataatgtg ctttagatga agccgataac
     2581 tgttttaatt gctcaatttg acgaattgct ttgtcaactt tgtctaataa atcttgctta
     2641 gataatatct cttttgaaat ttcagtatcc ttttcagatg cagcttgggc atcgtacggc
     2701 aagatattcg ttaaaatgat acttgacgcc atcattgtcg aacacgataa ctttacatat
     2761 aattgaaacg gtttccctcg atatttagcc atcaacatac tcctttctca cttacttcct
     2821 tcaaagaatt acatactatt atatacctgt ttacaagaaa tttacactta tctatctagt
     2881 tattgttgtt agtaattatc aacttattac ttagcttata tttaagtaaa caaaaaagca
     2941 tgacgtaata tcatattgtc catgtcgcta acatcatatt acgtcaaatc ttttaaatta
     3001 aatgatgctt tattttagac tgctttttct ttttagcttt cgagcgcctg tttaaaaact
     3061 tgctcgaatt gttcacgcga gatttcgtgt gcatgtgctt tttgtgctaa taaagcatct
     3121 cgaaactgtt gttgatcttt caaactttct aacatttgta ttaattggtc tttactttcc
     3181 attgttatct catcattatg ctcaaataag tgctctgata atgttacttt agcatggtgt
     3241 gcggtttgac gataacctaa aatcaacaac tcatagtcaa acgcttgttc caccgcattt
     3301 aaaatttcat taccctcatt gatatcaaga taaatatcac ataactggta tagttcattt
     3361 accctgtcaa tattaataga tgggtataag tgcacattag catattgatc aagttgcatt
     3421 agcttatcag acatctctgt aatagcagcg atgtgaaaat taaaatctgg taaagtttca
     3481 accaatacct tgatgttacg aagttgatcc gagttagtta atattacaat ttctttagta
     3541 tatctattac gactacgata gttatataga tatccgcctt gtaaaatacg agattgaacc
     3601 tttgcgtctg ctatattgag catcgtttca tattcgtttt tatctggaat aataatatta
     3661 caatgtcgtt tcatatcacc tttacacatc aattgcatat ttcccgggac attaccatta
     3721 cagtgttctt gccataccaa aacatcacta ccttttgatg gcaaattata taacactgaa
     3781 aatggtaggg ctagtgagtt aataacgaaa tgatgttccg taatttcaag ttgcttgata
     3841 aaaaataata cgaatgcgag ctttgaaggg aaaaagtaag acttcccttg ccaatccaat
     3901 atgacatcag atgttacaaa attttcataa atcacttctt taccttctgc tgtcatatat
     3961 ttcttcaaga tcgctttacg atttaaatcg taaacagttt gtgcaaattt aataccattc
     4021 ttagaataat aatcgacaaa tcggacacgt tgttggtcat caaaccattc gacacgacta
     4081 acaattctag ggcgctctcc actttgataa aatattttgc ctcgtagacg tcccatatca
     4141 ttaattgtag ccgaattgtt gttaccttta atttcccaaa aagctggtac agtaacctga
     4201 ttaaaaaatc gtggtttcat attttctgta ttatgattat ctgcaaaaaa ttgatacggt
     4261 gatataacat cgtccggtaa aaagccattg tcattgagta caattgttaa atcttcttcc
     4321 aacttactgg ctttaaaaga ctcatataac tttcgtgaat gatcgttaaa gtaatcaaat
     4381 aatttaatca tgtagcacct cttgaactaa tgtttcccat tttaaaataa tatcttgagt
     4441 cataaattgc tgtgccactt cataagagat gtcatgtggt gcctggggac cattgttaaa
     4501 atacattaca atggcatgag ctagttttgc gataacatca tccacactat cttcgtcggt
     4561 atcaaaaggt accaagtagc cattttcccc atctcgaata aaggttgggt taccataatt
     4621 cacatttaat ccaatcatac ctagtcctga gcctaccgct tccattagtg ttaacccaaa
     4681 accttcgcta gttgatgcag aaagaaataa ctcataatca ttataaattt catcaagttt
     4741 aacatgccct agtaaacgaa tataatcttg tgcgcggtgt gtatcaataa ttttacgcag
     4801 tcgcgtcttc tcgctacctt ctccataaat atcaaatgtt aattctggca cttgtcgttt
     4861 agccacgata accgctttga caagccaatc aatatgtttc tcatttgcta aacgagatgc
     4921 actaatcatc gcatatggct ttcttgataa tttaggatat gataacgcat caatgcttcc
     4981 caccggaata gtatagacac gtggacgata accttgatat tgctcaaatt gtcgacaaac
     5041 catatgattt tgaatatctg ttgctgtaat aaagaaatca atgtatttag cttttgaaaa
     5101 ttgatattca taataattgt tccatagtat atgctgctcg ctcatcatat tattactata
     5161 atgatcagca tgaatcacaa caccaacttt actatcacct ttatgctgca aaacagcctg
     5221 accaatatca gaagcgcggt ctaatatgac aatatcgtct cgggttaaat tcaatcgttg
     5281 taaaaagtat gcaataaatt ccgttttgtt atacaacacc gcatcttcaa acacatatat
     5341 agagctgtct ccatcaatat attcgttata agcgatggaa ccatcttcat tatagaattg
     5401 tcgcatatat aatttcgctt tattatcagc tggtgcataa tactcagaaa atatacgcgt
     5461 ataactataa aaatctttac gtactaacat actattaatt acaaattctg cacgatccac
     5521 aacatctttt tgttcatttt gtagataaca tgttacaaat gatgatttcc cattaaaata
     5581 tagacggact atcttaccat ttctttctct aaaactaatt tcatgaccaa gctcacgttc
     5641 aatgtcatct aacgtgtacg ttgttggtgc tatagaaata tcactaaaat actgatacaa
     5701 ccaaataact tcttgatctt taaacccaat gttttgcgtt aatgtctgta tgttctctga
     5761 ctgtataaaa tctaaaaaca caaatttagt gtcttgattt gtacgtctca ataatttagc
     5821 acggtaagct tgtgcatatt caacaccgct actcgcccag cctataccaa agtttatatt
     5881 atatattgtc atgcgctacc ccttttcatt tatggaaaat gtataactgg cataccctct
     5941 ttatcaaatg taatcatgct ttgacaaata tttttcacca ttcttttttt gatatttcgt
     6001 gtcataactt caaatgaatc taatgcaact ctatggtatt caaaaatagc gttacgttgc
     6061 ccattttgtc gttgattaac gcttgctttt aattgttgta aatagtcgac ttgttctaac
     6121 caacatgaat caattgcttt caaaaagact ttttgaacga aaatattata ataatatgca
     6181 ctttgcatgt ttttacgatt caaagctaat tgcttttcaa attgctctaa taaaaatgtc
     6241 actactgctt gcttatcttt aaaattaaca caagccacat ctttattaaa ttggaaactt
     6301 aaattttgat aaatatactc gacaacacgc gattttgtta gcaccttttc ctcatttaca
     6361 aacatttcaa atacatcttt agctaacgct ttaaaatctc gattctcagc atcatctatt
     6421 tctaaaactc gattgcgttc ctcgtataca agatctcgct gtatactaat gcttttttca
     6481 aattcattag ccatttcacg agctttaacc ccttgttctt ccgagatacg ctgcgcttta
     6541 actacaattt gcttaacttt gcgattaaac aaattacttt gcgataatcg ttgtgcatct
     6601 aatgaatata attgattatt ttccgctaaa ttactatcgc tccatcgctt aactaaataa
     6661 tcatctagtg aaatatatat acaagatgat cccggatccc cttgtctacc agaacgacca
     6721 cgtaattgcc tgtctacacg gctattttcc atatgttcat gaataataac agctaatcca
     6781 cctaatgctt cgacaccttc accaagttta atatctgtgc ctcgacctgc catactagtc
     6841 gcaacagtca tggaaccaat ttgccctgct tcagctatca tctgcgcttc ttttgcaaca
     6901 ttttgcgcaa tgagtaaatt attaggaata tccatttgga ataatacttt cgaaaagtat
     6961 tcagccgctt cagcagttct cgttatgagt aaaatcggtc gccccgtttc atgaagttca
     7021 actatatcat gaatcatcgc gatgtttttc tcatcaactg aacgaaacac tttatctggt
     7081 tcatcgatac gttgaatcgc tttatcagtt ggtgcttgta cgactatttt tgaatacaaa
     7141 tcaaagaact ctgattcgcc taattttcct gtagctgtca tacctgaaaa tgattcaaaa
     7201 agtttaaata aattctggaa ggtaattgtt gccataacac ttttatctgt tgaaacctcc
     7261 ataccttctt tcgcttcaat agcttggtga agtccagctt gcaacttagt tcccggtaac
     7321 atacgacctg taatacggtc aattaaaaca atatcaccat tatatacaaa gtaatcgaca
     7381 ttagattcaa acaaatattg tgcgcgcagt gctaaattaa tattacgcac taggaccatc
     7441 gcttgttcgc tatataaatc ttcaacatta aagtatgatt gtgccgcttc aataccttga
     7501 tttaacagcc atatttcttt tttggtcttc ttcattttaa aatgcacgtc ttcaatcaat
     7561 gtatctacaa actctttcac aatatgaaat agatttgatt gtaatcttgg tgcacccgaa
     7621 ataactaatg gtgtttgagc agcatctaaa atgattgaat ccacttcatc aataataccg
     7681 taatttaatt gtggtaaaaa tttcccttcc gcactatcag ccaaattatc aattaaataa
     7741 tcaaaaccga gacgtccatt agttgtatat ataatatcat gttcatatat attacgtttt
     7801 tccccttttt gatactcata atccacaata tcaacaaaac ctaatgaagc agttaaacct
     7861 aaccattcat ataatggttg catctcttca aaatctcgct tcgctaaata atcattcgtt
     7921 gtaattaaat atgttccttt tcccgaaaga gcatttaaat ataaaggcat cgttgccgtt
     7981 aatgttttac cttcgcctgt ttgcatctcc gcaatgttac cttcatgcaa tacaatcgct
     8041 ccgattaact gaacttcttt aggatacata cctaatactc tccagctcgc ttcacgtgcc
     8101 actgcataag cttcaggtaa caatgtatct agtgtatcaa ctcctgatgc taaacgttct
     8161 ttaaattcta ttgtcttttg ttttaacgca tcatcagaat atgatttaac ttcatcgctc
     8221 catgtattga tgcgtttcac tatttttcta atcgacttta gtcttaattc gtttatcgta
     8281 acatctagtt tatgtttcat ttacttcccc accattcagt ttcgatacat ctaagtaatc
     8341 taaaaatcgt actggattca ttaaacgtga catataattt agatggttgt cttgctcttc
     8401 tttaaaataa aactcgacat ttgtatcttt tagttcatta tttcctggga catgttctgt
     8461 aagccattct tttaaatcat catcttcatg gcttgtacga tatactttgc aatctaaatg
     8521 ctgagcgaca taagttgcaa aaacatttga ctttgaccca taactaatca aattaatagc
     8581 cgttagggta tcttgacttt gcaaatcatc ctttagttgc ttaatatttt ccttgatatc
     8641 gtcgtccatc caacgttcaa cgagccaaac atgaccaaac agtttcaaaa aatcattcga
     8701 aatagttgga taggtgtcag atggttctgc aataatgaca ttgatcctat catttccata
     8761 ttggttatcg tctatcttcg tcacccgcat gcttttatac tctaaatcat cttgatgcgt
     8821 catctctgta attgttaaac atctaaatat tagactcgtc gatgctgcat tcatcatttt
     8881 tattttataa gcataggctt catcaggata ttgaatcgta atactatttg actttacaat
     8941 ctcagtactt agttttgtgc catttttatt ataaaaaatg atgataaaat acactgaacc
     9001 agcaggcgtt gcatcaaaat caaaatgcaa tttataatgc tgtcctctac gcaaaattgg
     9061 taaacttggc gcacttttat attttgaaaa ttgctttaac atcaaccact catgaatcgg
     9121 taatccagag ggcatcaaag gatttataaa agtcacttca ccatttgaaa atgatacttt
     9181 agagccatac ataaatgtag tttgtgaaat ataattccaa gtaactttaa atgttttgtt
     9241 tttcagcatg ttgaactctc ccaaacttgt cttccaaaat aatgttgtaa aaattaacaa
     9301 accaacttgc aatggtaggt gaatcatcat tatgtcgccc aggaatactg cgattcatca
     9361 ctcttgcttg gtgtgctgtc aatacaggta atagctcttg aaatgcatgt ggatcataat
     9421 catcatgttg catatatgct atggcaaaaa cagtttgtga caatgatttc ttttgaaatg
     9481 tttgccaaaa tttttgattt aatgccttta tcgacgcttg agatgtatca ccttcattag
     9541 acaccaggac gtctaatgct gtaccgaact cttctggtct aagtaatcgc atatgttcag
     9601 caatcgttcc aatattaaca agtggtttac caacaataat tgcctgagga tttaactgag
     9661 caccataata caaggcacca aatgaaccca tagataaacc agataatatt aattcatgtg
     9721 atttaaaatt cagcttttct aatgtctcgt caataacatt gataatacct tgttcatatt
     9781 cagatgaacc gatataaaag ctaccacctt caacacgagg atcgccgata agtaaaaacg
     9841 gtgcattcat acgtttcatc atataatatc cttcgaaacc ttccgctgtt cgataaccac
     9901 taaaatatac gtttagtggc ggtttcatat caccagggtg gaaataataa ataaattcct
     9961 gtcgttgact atctacgaaa cgactaccac caagtaaaaa ttgacccatg tctaatctag
    10021 accatcgttt gtgtataggt cctaaatgta ccgtcccgtt cccacgcgcc ttaacagtta
    10081 cacttatata agcatcaaat ggtttcgcag gtatctctaa aggactgtct aacatatcat
    10141 cagttaatat gatttgttca attaatgcac catcagcgcc agtctgaatc aatctaaatg
    10201 tatattgcaa ctcgaccgca ccatcaatat caaattctgg ccatatttga atgactttat
    10261 ctttatcgta aacgagatta ttttgccaag atgcgatagg tttaaattct tttccaaatt
    10321 ctccactcaa cgtgagctct gaattacctt ggtatacgac atctccttta aaattcggat
    10381 gcacaagtgc taacttaggg gaaactttat ctccatactg tcctgagaag ctaactgcct
    10441 ctaatttatt attacgttct tcaatattcc ggtaatgtaa tggttgaaca acgtattttt
    10501 ggacattttc gtcttgttca tattcaactg accaaaatga ttcatcaaca tacgtattgt
    10561 atggttcgct tatcatttgt aataaattcg ttaatgtctc cgagtatggt gcttgaatat
    10621 agataaaatc aaagcgccct tctgcttcaa caatcgcttc aatagcctct acataaccac
    10681 tatcaaattc aaacaatcca atatcgaagt aatcccaact cacacctttt ttgtgttgaa
    10741 aaataggttc taaatcgtct cctccaattt gcaaaactct aaatttacgt ggcatcattt
    10801 tcaccttcta ttaactcatc gagctgatta ataatattct tagaagcata tgcatctatt
    10861 aattttaaag aataggcgta cgcataattc caatttttca aataaaataa ataataattt
    10921 aacgcatcat ctaattcatc aactgtattt ataatacggc cattgtcata atcagagacg
    10981 taatctgttt gttgaccatt aatttgtgga atcccagcgc taattgcact aatttgtaaa
    11041 tacaaatcag gttcttttga catatctatc acaagtcgca acgtccgcaa tgcttctaca
    11101 acatcatgtt cagcatgtat cgtcttaaca gcaatgatgt catcttgatc ttcaggtgtc
    11161 attaatgctg aaacattaac atccgcattc tgtttagctt ggtattcctc atttaccgac
    11221 gtaatacatt cacgaagcca catcggtatg tcattttgat ggcgcgataa taaaattaaa
    11281 cggtaataat cttcctgtgc gatataatcc acaagtcgtt gcatcatttg ttgcaaatca
    11341 gcgtcactca taccatctat ccatacacct ataaatgttt ccatcaattg actacttata
    11401 ttaggtgatt gtctcgtttc aaatggtgtg attcgaatca ttgtattctc ccgctgatac
    11461 ttctcttgat gactaattaa atcacgttct agttgcacac tatcaacaat taaatgggca
    11521 ttctttacga tagattgata ttcctcatct gacacagttt catttctatt tttaaaaaat
    11581 gaataactta acaatttcgc tggaatatga ttggctattt gtcgattgtg cctagcatct
    11641 gaagccacaa tcacatgatc atcttcatgt atttgttgtg caatcattgc ttgaaatttt
    11701 tcttcaatta gttgagccat attgttatat tctgtttgtt gatagtgatg ttgatatctt
    11761 tttgatacag tgactctgcc atttttcaaa tcttcatgaa gtacacaatc tccattaatc
    11821 gttaaatatt gttggtaaga agcctctccc tgatcatcaa aataacgtat cgctgataaa
    11881 taacctctgt catcaaaaat ataacgccgt tgtaactgat ctctttcaaa ttcttcaaac
    11941 caaattgaat acccttcttg actaaaataa atatttgtat aggtctgttc actcgtcaca
    12001 cattttaata aatacggcgt gtacacaaac tcaacatcat ccggccattt taagtgatga
    12061 taattaatcg cttgtggcgc atggtgactg aatccttgaa tttcatcaaa cacagacgaa
    12121 tactttgttt catataagtc atatcgatgt aaaaatgttc ttaaatttgg tgcatgattg
    12181 agaacaatca gttgataatc taagtcattt tcaaggtgca ttcccattaa actaatcata
    12241 tcgtcaaatt ccgtcttatt ttgtagttga taatacggca cagtcgtgtc ttgccaccat
    12301 cgttggtcat cgtaccaagc tggaataaag tatttcataa ttacctcctt accaatactg
    12361 gtttaaaaat ggcttatatt tatcaaaata taaatatgta cgaattgttt ctgcaatatt
    12421 aatactgatg taaactaata caatcagttg tactgagaaa taaatttcag tagataaatg
    12481 cggtacaaac aatgtgaaat aaagcggtat accaataatg actgtaacta atgccgatcc
    12541 aaaccaacat acgcgtcttg cttgataatt taaataacgt tctgtatcct taccaggttt
    12601 aactcctgaa aaataattgc cactctttaa gaaatctttg gatttttgtt tagtattgat
    12661 taaaaatctc gataaaaaat aacccaataa catttgaatc actaaatata ctgaaatacc
    12721 tactggacta tcaaatgtca gcattggcat gtcatctgat atgcttttat taaacataga
    12781 taaaataaaa tgaatgccac ttttcaagaa aacaaatgct gaaatactca tcattaaagt
    12841 aatactgcct gcagggttaa ctttccaaga taaataagat ttcatatttg ttgcggaaac
    12901 gttcattaaa tcgatatatg gtattctcac ttctactaat tcaatgaata ataagataaa
    12961 caatgtgatt atcacaagga tgattaacaa cgcaatcaca atatgacttg catctatata
    13021 ttccattttt tgatgcatca ttgatttaat aatgctaacc attacaatcg gcattggtcc
    13081 tgcgatgccg taacgactat ttttgtcagc taaccaaact aataacatcg ttccagtaac
    13141 caaaatcaat attgttaagt aaatattgtc ttgatgaaca cgttctttcg aaacatattc
    13201 atgaatcaca aaataacttt gaatcacact taaaattaat gttaagatac gctctttata
    13261 atgcttctca agacttgttt gtttcatgta tttatccata ttccgataag aaatcagcat
    13321 caagataatc attgatgtta accacggacc taatcctaaa gtgaaaatgt ttaaagtatt
    13381 aacgtctcca cccatattag aaatagctat tttaaaaaat gactcatgtt ttacttgcat
    13441 atcgttata
//
LOCUS       DAOBBH010000060        13356 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.060, whole genome shotgun sequence.
ACCESSION   DAOBBH010000060 DAOBBH010000000
VERSION     DAOBBH010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13356
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.060"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            159..854
                     /locus_tag="P6L01_001254"
     CDS             159..854
                     /locus_tag="P6L01_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDP3030331.1"
                     /translation="MGVLTIEHLTKKIGNKTILEDVSFKLKRGQIVGLVGANGAGKTT
                     LMKVILGYSSFQSGNFNVINSKDSKSNIGALIENPGIYPFMSGYENLKLLNESKNTQD
                     IDKIVSQLHMDEYIHKKAKTYSLGMKQKLGIAIAFLNEPQFIILDEPMNGLDPKAVRD
                     VRELIVQKSQEGVTFLISSHILSELVKITNSILIINKGKIVTETSEEELKQFKDNDLE
                     NVLLEIIEREDQA"
     gene            856..1626
                     /locus_tag="P6L01_001255"
     CDS             856..1626
                     /locus_tag="P6L01_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3030332.1"
                     /translation="MGTLIKQECFKLFKKKSTFIAPIVFILLMVAQGYIATKYNEIFT
                     PQESFTSAYNGFSWFAFLLIIQASTIISMEFHYGTIKNLLYREYSRTTMIVSKIITLF
                     IISLIYFVITIIASIVIGSLFFNDLNIFESSGNQLSLLNQLLLVSLGTFVGVWLVLSL
                     TLLLSSATNSTGVAIAVGIVFYFASSILAVIQTALLEKIDWLKWNPINMMNIMLQTVE
                     KGFSKSTKLELHELFIGNIAYISIFLILVVFIFKKKNI"
     gene            1999..3963
                     /locus_tag="P6L01_001256"
     CDS             1999..3963
                     /locus_tag="P6L01_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HDP3030333.1"
                     /translation="MTQITEKELKKKYLDLLSQNFDTPEKLATEIINLESILELPKGT
                     EHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKERLSTKELNDLTALVYYPEDKLKL
                     IKSDFQSCGQLNVWYITTIEHLIELIKYCSSKYTRSKLRKALPKQYVYIIEELLYKSN
                     EYQNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIM
                     DTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIIEDAYGINLRPL
                     LTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPIIKRRP
                     NFEMEERLVLEKVNYDTNEITVYGNTYPLKDTCFQTINRNNPAELLPEEEEVMNKLLL
                     SFQQSEKLRRHMSFLMRKGSLYLPYNGNLLIHGCIPVDENGEMESFEIDGHTYSGQEL
                     LDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHK
                     EEKNPYYHLREDVNMVRKMLSDFGLNPDEGRIINGHTPVKEINGEDPIKADGKMLVID
                     GGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRVVDKELQ
                     RKKIRDTNIGKELQAQIDILKMLMHDRYLD"
     gene            4305..5384
                     /locus_tag="P6L01_001257"
     CDS             4305..5384
                     /locus_tag="P6L01_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030334.1"
                     /translation="MRILKESIIVAFAFVGVVVGAGFATGQEIFQFFTSHGAYSISGI
                     IVTGLLITLGGMVVMHTGHHLKSRNHSDSINYFLYPSIARGFDIILTMFMLSLAIIMT
                     AGGASTIHQSFNLPYWLSALILVAFILATLFLKFDRLIAVLGGVTPFLIAIVIMIAVY
                     YFTTSHLDFTAANNDAQIHKQKSLSPGWWFDAINYASLQIAAAFSFLSVMGSKVKYRD
                     STLYGGLIGGLIITFLLMMINLGLISQFDKIKHVDLPTLKLATQMSPSIGIIMSVIMI
                     LVIYNTVVGLMYAFASRFSVPFSRRYFIIIITMAVITYISTFIGFISLIGKVFPIMGL
                     FGFILLIPVLYKGLIKRITGKSHID"
     gene            complement(5489..6082)
                     /locus_tag="P6L01_001258"
     CDS             complement(5489..6082)
                     /locus_tag="P6L01_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDP3030335.1"
                     /translation="MEHIFREGQKGAPTLILLHGTGGDEFDLLPLGEALNENYHLLSI
                     RGQVSENGMNRYFKRLGEGVYDEEDLEFRGQELLAFIKEAAERYEFDIEKAVLVGFSN
                     GSNIAINLMLRSEAPFKKALLYAPLYPVEVTSTKDLSDVSVLLSMGKHDPIVPLAASE
                     QVINLFKTRGAVVEEVWVNGHEITETGLTAGQQILGK"
     gene            complement(6113..7072)
                     /locus_tag="P6L01_001259"
     CDS             complement(6113..7072)
                     /locus_tag="P6L01_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001628197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDP3030336.1"
                     /translation="MINNHELLGIHHVTAMTDDAERNYKFFTEVLGMRLVKKTVNQDD
                     IYTYHTFFADDVGSAGTDMTFFDFPNITKGQAGTNSITRPSFRVPNDDALMYYEQRFD
                     EFGVKHEGIQELFGKKVLPFEEVDGQVYQLISDELNDGVAPGVPWKNGPVPVDKAIYG
                     LGPIEIKVSYFDDFKNILETVYGMTTIAHEDNVALLEVGEGGNGGQVILIKDDKGPAA
                     RQGYGEVHHVSFRVKDHDAIEAWATKYKEVGINNSGIVNRFYFEALYARVGHILIEIS
                     TDGPGFMEDEPYETLGEGLSLPPFLENKREYIESEIRPFNTKR"
     gene            complement(7150..7584)
                     /locus_tag="P6L01_001260"
     CDS             complement(7150..7584)
                     /locus_tag="P6L01_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDP3030337.1"
                     /translation="MDRTKQSLNVFVGMNRALDTLEQITKEDVKRYGLNITEFAVLEL
                     LYNKGPQPIQRIRDRVLIASSSISYVVSQLEDKGWITREKDKDDKRVYMACLTEKGQS
                     QMADIFPKHAETLTKAFDVLTKDELTILQQAFKKLSAQSTEV"
     gene            7810..8094
                     /locus_tag="P6L01_001261"
     CDS             7810..8094
                     /locus_tag="P6L01_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDP3030338.1"
                     /translation="MSNLEIKQGENKFYIGDDENNALAEITYRFVDNNEINIDHTGVS
                     DELGGQGVGKKLVKAVVEHARENHLKIIASCSFAKHMLEKEDSYQDVYLG"
     gene            8265..9071
                     /locus_tag="P6L01_001262"
     CDS             8265..9071
                     /locus_tag="P6L01_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDP3030339.1"
                     /translation="MAFHDKTATQVTNIELNVRDLDLMTTFYKNILGLSVKSSDDNTT
                     VLSVGTGGHTLTLHLLEDGRQTSPREAGLFHIAFLLPTTEDLANFLYFVAQKNMGIGA
                     GDHLVSEALYFNDPEGNGIEVYRDRPSSSWEWQNGKVKMDTLEVDSQTLLTHRTDEGW
                     QGMPAKGMIGHLHLKTHDLDAAYQCYIEQLGFQHVSDFPRALFMSTNHYHHHIAANTW
                     QSNQARQNNAQSYGLTHVDIYQPDASTHTFTAPEGFDITVHSNTDLVPEK"
     gene            complement(9192..9863)
                     /locus_tag="P6L01_001263"
     CDS             complement(9192..9863)
                     /locus_tag="P6L01_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDP3030340.1"
                     /translation="MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPS
                     SLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHML
                     RDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGI
                     DSCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQDPPKNKTRQAYEDVIEW
                     VGPKE"
     gene            10133..11125
                     /locus_tag="P6L01_001264"
     CDS             10133..11125
                     /locus_tag="P6L01_001264"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDP3030341.1"
                     /translation="MTKIMFFGTRDYEKEMALNWGKKNNVEVTTSKELLSSATVDQLK
                     DYDGVTTMQFGKLENDVYPKLESYGIKQIAQRTAGFDMYDLDLAKKHNIVISNVPSYS
                     PETIAEYSVSIALQLVRRFPDIERRVQTHDFTWQAEIMSKPVKNMTVAIIGTGRIGAA
                     TAKIYAGFGATITAYDAYPNKDLDFLTYKDSVKEAIKDADIISLHVPANKESYHLFDK
                     AMFDHVKKGAILVNAARGAVINTPDLIAAVNDGTLLGAAIDTYENEAAYFTNDWTNKD
                     IDDKTLLELIEHERILVTPHIAFFSDEAVQNLVEGGLNAALSVINTGTCETRLN"
     gene            complement(11372..12172)
                     /locus_tag="P6L01_001265"
     CDS             complement(11372..12172)
                     /locus_tag="P6L01_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDP3030342.1"
                     /translation="MVKAIAVDMDGTFLDSKKTYDKPRFEAIFTELRNRDITFIAASG
                     NQYAKLKSIFGDRDMYFISENGAVIYKGNELYNYKSFNRQVFQQVVDYLNMNQSIDQL
                     VICGLKSAYILKHTSEAFKEDTRFYYHQLKEIDSLQQLPEDDYVKIAFNINRETHPNV
                     DEEVATQFSNDIKLVSSGHDSIDIIMPNMTKGQALKRLLDKWEMSPSELMAFGDANND
                     KDMLAFAKHSYVMENSHDEELFNIASAVAPSNDKQGVLTIIEQEVLNK"
     gene            12492..13127
                     /locus_tag="P6L01_001266"
     CDS             12492..13127
                     /locus_tag="P6L01_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDP3030343.1"
                     /translation="MKLNNYSLKVKNKQLVDNCDLNFYLGQINHIIGKNGVGKSLLAK
                     DFLLNNSGNIPKSISQNVTLISSSSNIPNDITKDFLLSLLKSKFENNRQTFDKIYNIL
                     NIEAIPSNVLLKNLSDGQKQKLKLLSFLLEDHDLIILDEVTNALDKKTVNEIYEFLND
                     FIQSHQTKTIINITHNLSDLSALPGKYFIFKDLQIEEYQSKEEVINDYINL"
     gene            13133..>13356
                     /locus_tag="P6L01_001267"
     CDS             13133..>13356
                     /locus_tag="P6L01_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HDP3030344.1"
                     /translation="MSTLKLEFKKSISNKIIYTLGVLFIFLFLLGYFLPIGIDKVKNL
                     SYGQFFFSSYTVATEFGFLLFSFVIAYFIN"
BASE COUNT         4409 a         2306 c         2041 g         4600 t
ORIGIN      
        1 ccatttgcac gctacaaaaa tactcgtgtt catatttata ttttatatta ttaaagtttt
       61 ttaaatccat atttttataa cacttgctat gttataaaat taattttata tataaaatct
      121 taagattcag attatttaat agcaaaggag atagtgatat gggtgtttta acaatagaac
      181 atttaacaaa gaagataggc aacaaaacga ttctcgaaga tgtatcattt aagctgaaac
      241 gcggacaaat agttggtctc gttggagcga atggtgcagg taaaacaact ttaatgaaag
      301 ttatattagg ttactctagt ttccaaagcg gaaattttaa tgttattaac agcaaagaca
      361 gcaaaagcaa tatcggtgca ttgattgaaa atccaggaat atatcctttt atgtctggat
      421 atgaaaactt gaagttattg aatgaatcaa aaaacactca agatatcgat aaaattgtct
      481 cacaacttca tatggatgaa tacattcata aaaaagctaa aacgtattct cttggtatga
      541 aacaaaaatt aggaattgct atagcatttt taaatgaacc tcaattcatt atcttagatg
      601 aaccaatgaa cggcttagat ccaaaagctg tgcgagatgt acgtgaattg attgtccaaa
      661 agtcgcaaga aggtgttact ttcttaattt cgagtcatat tttaagtgag ttagttaaaa
      721 tcacaaactc tatccttatt attaacaaag gtaaaattgt tacagaaaca tcggaagaag
      781 aacttaaaca atttaaagat aatgatttag aaaatgtatt actagaaatc atagaaaggg
      841 aggaccaagc ataaaatggg aactttaatt aaacaagaat gtttcaaatt atttaaaaag
      901 aaatcaactt ttatcgcacc tattgtcttc attctactaa tggttgctca aggttatatc
      961 gctacaaaat acaatgaaat ttttacgcca caggaatctt tcacatctgc ttataatggt
     1021 ttttcatggt ttgcattttt attaattatt caagcaagta caatcatttc aatggaattt
     1081 cattacggta cgattaaaaa tttactctat cgtgaatatt caagaacaac tatgattgtt
     1141 agcaaaatca tcacattatt tattatttct ttaatttatt ttgttattac aattattgct
     1201 tcaattgtta ttgggtcttt attctttaat gatttaaata tatttgaaag tagcggtaat
     1261 caattatctt tattgaatca attattatta gttagtttag gtacatttgt tggcgtttgg
     1321 ttagttttaa gcttaacgtt gctattatca tctgcaacta attcaacggg tgtagccatt
     1381 gctgtaggta ttgtttttta ttttgcaagt tctattttag cagttattca aacggcactt
     1441 ttagaaaaaa tagactggct aaagtggaat cctattaata tgatgaatat tatgcttcaa
     1501 acagttgaaa aaggctttag taagtcgacg aaattagaac ttcatgaatt gtttattggt
     1561 aatattgctt atatttctat tttcttaata cttgtagtat ttattttcaa gaagaaaaat
     1621 atttagtaac ttaaagtatt agatgaataa atacacacat attccatcgt aattcaaaat
     1681 cattttcaaa tcccttcacc caaataatgg tgcggggatt tttttatcca aattttggca
     1741 attccacatt ttacatatcg taatttttta ggaaactagt gaatataaca aattcctcct
     1801 ctcattttta aaataaatat atcactttcc cacttttact taactaaact gcaacgactc
     1861 ctaataccaa aatcctgccc tctattttta tcaatccaag catacttatt gaaaaatgtt
     1921 aacgttttct tgataatcat tgtaagcgca tttattttat aaactaacgt ttgaaatata
     1981 ctacaggagt gacacgtaat gactcaaatt actgaaaaag aattaaaaaa gaagtattta
     2041 gatttactat cccaaaattt tgatactcca gaaaaacttg caactgaaat tatcaattta
     2101 gaatcaattt tagaattacc taaaggtacg gaacatttcg tcagcgattt acatggtgaa
     2161 tacgaagctt tccaacacgt attacgcaac ggttctggga atgtgcgagc gaaaatcaat
     2221 gatattttca aagagagact ttcaactaag gagcttaatg atttaactgc tcttgtctac
     2281 tatccagaag acaaattaaa attgattaaa agtgatttcc aaagttgcgg tcaacttaat
     2341 gtctggtata tcacaacaat cgaacattta attgagttga ttaaatattg ttcttcaaaa
     2401 tatacgcgtt caaaactgcg taaagcattg ccaaaacaat acgtttatat tattgaggaa
     2461 ctactgtata aaagtaatga atatcaaaat aaaaaatcat attacgaaac acttgttaac
     2521 caagtaattg aacttaaaca ggcagatgat ttaattattg gactcgctta ttccgtacaa
     2581 cgcttagtcg tcgatcattt acacgttgtc ggtgatattt atgatcgtgg accacaacca
     2641 gataaaatta tggatacact gattaattat cattccctag atattcaatg gggtaatcat
     2701 gatgtgcttt gggttggagc ctatgctggg tcaaaagtat gcttagcaaa cttacttcga
     2761 atttgtgcac gctatgataa tttagatatt atcgaagacg cttatggcat taatttaaga
     2821 ccactgctta ctttagctga aaaatactat gacgcagata atcctgcttt taagcctaaa
     2881 aaaagacctg acaaacacga acgtttaact caacgtgaag aaagtcaaat tactaaaatt
     2941 catcaagcta ttgcgatgat tcaattcaag ttagaaatac caattattaa acgtcgtcca
     3001 aatttcgaaa tggaagaacg tcttgtgctt gaaaaggtta attatgatac aaatgaaatt
     3061 acagtttatg gtaatacata cccgttgaaa gacacatgtt tccaaactat caatcgtaat
     3121 aatccagcag aattactacc tgaagaagaa gaagtcatga ataaactatt attgtcattc
     3181 caacaatctg aaaaattacg tcgtcatatg tctttcttga tgcgtaaagg ttcgctttac
     3241 ttaccatata atggtaattt actcattcat ggttgtattc cagttgatga aaatggtgag
     3301 atggaatcat ttgaaattga tggtcatact tacagcggcc aagaattatt agatgtgttt
     3361 gagtatcatg tccgtaaatc atttgatgaa aaagaaaata ctgatgactt atcgacggat
     3421 ttagtttggt atttatggac tgggaaatat tcgtcattat ttggtaaacg cgcgatgact
     3481 acgtttgagc gatattttat tgcagataaa gcttctcata aagaagaaaa aaatccatac
     3541 tatcatttac gtgaagatgt gaatatggtt cgtaaaatgc tcagtgattt cggattaaat
     3601 ccagatgaag gacgcattat taatggtcac acaccagtga aagaaatcaa tggcgaagat
     3661 cctatcaagg ctgatggaaa gatgcttgtc attgatggtg gtttttcaaa agcttatcag
     3721 tcaacaactg gcattgcagg ctatacattg ttgtataatt cattcggaat gcaactcgtt
     3781 gcacaccaac aatttaacgc taaagaaaaa atactttccg aaggtatcga tgaactttct
     3841 ataaagcgcg ttgtagacaa agaattacaa cgtaagaaga ttcgtgatac caatattggt
     3901 aaagagcttc aagctcaaat agatattttg aaaatgttaa tgcatgatcg ttacttagac
     3961 taaagacgac attttatgca tgtattgtat actgttgtat tgatgcggtt tacgcattgg
     4021 ctacgcaaac gcgatgataa ataggcgacc tagctacacc gaaaaaaatc ccctcactat
     4081 tgcgagtagt gaggggattg gtgtatctat gctaattcct gttaactgta ttataacaac
     4141 tttacagcaa aaaaatgcaa aatcaactaa taaattaatg aaatgaccga cgccctcgct
     4201 tctacttcaa tttaacaaaa tactatatta gtactatatt tttcattaac atgtatgtta
     4261 cattttctat attacttaaa attacgaagg gacattttta aatcatgcgt atcttaaaag
     4321 agtccattat tgtggcattt gcctttgttg gtgttgtcgt tggtgccggc tttgctactg
     4381 gtcaagaaat tttccagttt ttcacaagtc atggcgcata tagcatttca ggcattattg
     4441 taacaggact attgattact ttaggtggaa tggttgtcat gcatacaggt catcatctaa
     4501 agtccagaaa tcattctgat tcaattaact atttcttata cccctctatt gcaagaggtt
     4561 ttgatattat tttaacaatg tttatgttgt ctttagctat tattatgact gcaggtggtg
     4621 cgtcaaccat tcatcaaagt ttcaacttac cgtattggct gagcgcactc atattagtcg
     4681 cctttatttt agcaacactg tttctaaaat tcgatcgttt aattgctgtg cttggtggag
     4741 ttactccatt tttaattgca attgtcatta tgattgcggt ctactatttc acaacgagtc
     4801 atcttgattt tactgccgct aataatgatg ctcagattca taagcagaaa tcattatcac
     4861 ctggatggtg gtttgatgcg attaactatg caagcttgca aattgctgct gccttcagct
     4921 tcttatcagt gatgggtagc aaagttaaat atcgtgactc aacgttatac gggggcttga
     4981 ttggcggttt aatcattaca tttttactca tgatgattaa cctaggttta atttctcaat
     5041 tcgataaaat taaacacgtc gatttaccta cattaaaatt agcgacacaa atgtctccgt
     5101 caattggtat tattatgtct gtcattatga tacttgtcat ctacaatact gttgttggat
     5161 taatgtatgc atttgcgtca cgtttcagcg ttccattcag cagacgttac ttcatcatta
     5221 ttattacaat ggctgtcatc acttatatta gtacatttat cggtttcatt tcattaattg
     5281 gaaaagtatt ccctattatg ggattgttcg gtttcatctt actcatacct gtactctata
     5341 aaggtttaat taagcgtatt accggcaaat ctcatatcga ttaaaacatc tatactattg
     5401 taaaatttat tacagcactt caaaatcaaa aaagcgggca gatgaaaagt aaaaatcttt
     5461 tccatctgtc cgcttcttaa tagaactgtt atttcccaag tatttgttga ccagccgtta
     5521 atccagtttc tgtaatttca tgaccattca cccaaacttc ttcgactact gccccacgtg
     5581 tcttaaacaa gttaatgact tgttcacttg cagctaatgg cacaattgga tcatgtttcc
     5641 ccatagaaag caacacactt acatctgata aatcctttgt tgacgttact tcaactgggt
     5701 ataacggtgc atataacaat gcttttttaa atggtgcttc cgaacgcaac attaagttaa
     5761 tcgctatatt tgatccattt gaaaatccaa caagtactgc tttttcaata tcaaattcat
     5821 aacgttcagc agcttcttta atgaacgcta acaattcttg tccacgaaat tccaaatctt
     5881 cttcatcata aacaccttca ccaagacgtt tgaaataccg gttcatccca ttttctgaaa
     5941 cttgtcctct aatacttaac aagtgataat tttcattcaa tgcttcgcct aatggcaata
     6001 aatcgaattc atcgccacct gtaccatgca acaatattag cgttggtgca cctttttgtc
     6061 cttcacgaaa aatatgttcc atcacaaatc ctcctcattc caattaatca tgttaacgct
     6121 tagtattaaa tggtcttatt tctgattcta tatactctct tttattttct aaaaatggtg
     6181 gcaatgataa accttcacct aatgtttcat acggttcatc ttccataaat cctggtccat
     6241 ctgttgaaat ttctattaaa atatgcccca cacgtgcata taatgcttca aaatagaaac
     6301 gattaacgat gcctgagttg ttaataccta cctctttata tttcgttgcc cacgcttcta
     6361 ttgcatcatg atctttcaca cgaaatgaca catgatgtac ctcaccataa ccttgacgtg
     6421 ctgctggccc tttatcatct tttattaaga ttacctggcc accattgccg ccttcgccaa
     6481 cttcaagtaa tgcgacatta tcttcatgcg caatagttgt cataccgtaa acagtctcta
     6541 aaatattttt aaagtcgtca aaataactta ctttaatttc aatgggtcct aatccataaa
     6601 tcgctttatc tactggaacc ggtccattct tccaaggtac accaggtgct accccatcat
     6661 ttaactcatc tgaaattaat tgatacactt ggccatcgac ttcttcaaat ggcaacactt
     6721 ttttaccaaa taattcttga ataccttcgt gtttaacacc aaattcatca aagcgctgtt
     6781 cataatacat taatgcgtca tcgttaggca ctctaaaaga cggtcttgta atggaatttg
     6841 ttcctgcctg cccttttgta atatttggaa aatcaaagaa tgtcatatct gtacctgctg
     6901 aacctacatc atctgcaaaa aatgtatgat acgtataaat atcatcttga ttgactgtct
     6961 ttttaactaa acgcatgcct aatacttctg taaaaaattt ataattacgt tctgcatcat
     7021 ctgtcattgc tgtgacatgg tgaataccta gtaattcatg attatttatc attttatata
     7081 ccctctttat atttttagtg tatttatctc gaaatcgaga tattttaatg taaattttta
     7141 gtgcacgcct tacacttctg tagattgtgc acttagtttc ttaaacgctt gttgtaagat
     7201 tgttaattca tcctttgtta acacatcaaa cgcttttgtt aatgtctcag catgcttagg
     7261 gaaaatatct gccatttgac tttgaccttt ttcagttaaa caagccatat atacacgttt
     7321 atcatcttta tccttttcac gtgtaatcca acctttgtcc tctaattgac ttacaacata
     7381 tgaaatgctg ctacttgcaa ttaatacgcg gtctctaata cgttgaattg gttgcggacc
     7441 tttattataa agcaactcga gcactgcaaa ttcagtaata tttaagccat atcgctttac
     7501 gtcttctttt gtaatttgct ctaatgtgtc taacgcccta ttcattccga caaagacatt
     7561 gagcgattgt ttcgttcgat ccatatcatc accaactttt taatttattt cgatttcaag
     7621 ataaattata tagctgaatg gatttaatgt caagatacgt gttttcatct ttatttcaaa
     7681 ttgcgcttaa taaaaacgac tacataaatg cggcaatgtc gtcacatgac tgtcatgcac
     7741 cttttacttc gtttaagtta aaatagaata gggaaatatt ataatcaaac atgataggag
     7801 ttgatatgta tgagtaacct tgaaatcaaa caaggcgaga acaaattcta tattggtgat
     7861 gatgaaaata atgctttagc tgaaatcaca taccgttttg tggataataa tgaaattaac
     7921 attgatcata caggcgtatc tgatgaactt ggtggtcaag gtgttggcaa aaaactagtt
     7981 aaagcagttg ttgaacacgc tcgagaaaat catttaaaaa ttattgcctc atgttcattt
     8041 gccaaacata tgttagaaaa agaagattca tatcaagatg tatatcttgg ttaaataaat
     8101 tttgcgatgg tgcaactgta ccatcgcttt tttgttcgca tcaatataaa attgcaaaac
     8161 gactagttat tttgcatttc aaactttatg ctatcaattc attttctttt ctggtatact
     8221 ttttatacta acttatagaa aaatagaaaa cgaggcgatg cataatggca tttcacgata
     8281 aaacagcaac acaagtgaca aacattgaac tgaacgtaag agacttagac ttaatgacaa
     8341 cattttacaa aaacatttta ggactatccg ttaaaagttc tgacgataac acaaccgtat
     8401 tatctgttgg gactggtggc catactctaa cgttacattt attagaagac ggccgtcaga
     8461 cttccccacg tgaagcagga ctttttcata tagcattttt attaccaact actgaggatc
     8521 tagctaactt cttatatttc gtggcacaaa aaaatatggg cattggcgct ggtgatcatt
     8581 tagtaagtga agctttatat ttcaacgacc ccgaaggtaa tggtattgaa gtctatcgcg
     8641 atagaccttc atcttcatgg gaatggcaaa atggcaaagt taaaatggat actttagaag
     8701 ttgatagcca aaccttatta acacatcgta ctgatgaagg ttggcaagga atgccagcaa
     8761 aaggcatgat tggacactta catttaaaaa cacatgattt agacgcagca tatcaatgtt
     8821 acattgaaca attaggattc caacatgtgt ctgattttcc acgtgcgcta tttatgtcaa
     8881 cgaatcatta tcaccatcat attgctgcta atacttggca atccaatcaa gcaagacaaa
     8941 acaatgcaca aagttatgga ttaacacatg ttgatattta tcaacccgac gcctcaacac
     9001 atacatttac ggcaccagaa gggtttgaca tcacagttca ttcaaatact gaccttgttc
     9061 cagaaaaata atcatccata ctttattaat acaatttttt cacgcaaaaa cctgcgattc
     9121 atttatgtaa tcgcaggctt tttttattgc taacttttta attttatata tttagacata
     9181 ctgtattcta tttattcttt tggtccaacc cattcaataa catcttcata tgcttggcgt
     9241 gttttatttt tcggtggatc ttgttgtctg tagccaaatg caaccatcac tgataaacca
     9301 aattgctcag tatctaagat acctttattt gctaaaatgt ctgtcactgt atccagacta
     9361 aaaccttcca tcggacatga atcaatacct aacaatgcgg ctgtcgtcat catattgcct
     9421 aatgcgatat acgtttgttt acttgaccag tcatacaagg cttgatcatt atcagaaata
     9481 tggaaatctg cttggaatgc atcgaatttt tgttcaacag ctgggatcgt ttgtgcctca
     9541 tattttttaa tatctcttaa catatgttgt acatacggtg atcttgacgt tacattttta
     9601 cgcgcaaaaa ttagcacaaa atggctcgct gtatctaatt gttttgctgc gccccaactg
     9661 tgcgctttta attcatcacg taacgcttga tcttgaatca cgacaaactt ccaaggttct
     9721 aacccaagag aacttggaga caatctacct gactctaata ttgtttcaaa atcttctttc
     9781 gaaactttct tttgtggatc gaattgctta gtcgcatgtc tgaaatgaaa tgcatccata
     9841 attgtttgat tcatattgct cattgaacat agcctccaat ttttatattt attactatac
     9901 aaattatact tcatgtttta aaaagactaa agtaatccgt ttgcaattcg ctataacttg
     9961 tgcacatgtt ataagttttc tgcaataaaa cacacttttg aattttggaa aatttagtgt
    10021 tataatatat ttgtgatatt tttcacaatt aaattgtggc gtgattttaa tgtacataat
    10081 ctttaatttc actcatttac ttttcaatct tttaaaagcg aggtttttaa taatgacaaa
    10141 aattatgttc tttggtacgc gtgattatga gaaagagatg gcattaaatt gggggaaaaa
    10201 gaataatgtc gaagtaacta cttctaaaga gctattatca agtgctacag tcgatcaatt
    10261 aaaagattac gatggcgtaa ctacaatgca atttggtaag ttagaaaatg acgtttatcc
    10321 taaattagaa tcttacggta ttaaacaaat tgcacaacgt actgctggat ttgatatgta
    10381 tgatttagat ttagctaaaa aacacaacat tgtgatatct aacgttccta gttattcacc
    10441 tgaaacaatt gcagagtatt ctgtttctat cgccctacaa ttagtgcgtc gcttcccaga
    10501 tattgaacgc cgtgtacaaa cacatgattt tacttggcaa gcagaaatca tgtctaaacc
    10561 agttaaaaat atgactgttg caattatcgg tacgggtcgt atcggtgctg ctacagctaa
    10621 aatatatgca ggatttggtg ctacaattac agcttatgac gcttatccta ataaagattt
    10681 agacttttta acttataaag atagtgttaa agaagctatt aaagatgccg atattatttc
    10741 tttacatgtt ccagcgaaca aagaaagcta tcatttattc gataaagcaa tgtttgatca
    10801 tgtgaaaaaa ggtgcaatct tagttaacgc agcacgtggt gcagtcatca atacacctga
    10861 tttaatcgct gcagtgaacg atggtacttt gttaggtgct gcgattgata cttatgaaaa
    10921 tgaagctgca tacttcacaa atgactggac taataaagac attgacgata aaacattatt
    10981 agagttaatc gaacatgaaa gaattttagt aacaccacat attgctttct tctctgatga
    11041 agcagtacaa aaccttgttg aaggtggttt aaacgcagca ttatctgtca ttaacactgg
    11101 tacatgtgaa acacgtttaa attaatatat atttaaaagc aaaggcgcac catgtgcctt
    11161 tgctttttat tttgtttatc tgtatcaaaa tcggtttgat taaaatctgc tttattttat
    11221 catctattca actgattgta gtgcgcttat tttactatgg cattattgcc taacctgttt
    11281 gagatatatt aatgtttgat gattcgtatt caaagttttc atttaacatg tatttagtat
    11341 catgatagcc gcttcattga tgatgtgcta cctacttgtt caatacttct tgttcgataa
    11401 tcgttaaaac accctgctta tcattacttg gagcgacagc cgaggcaata ttgaacaatt
    11461 cttcatcatg actattttcc atcacataac tatgcttagc gaacgctagc atatctttat
    11521 cattattcgc atctccaaag gccatgagct ctgaaggaga catttcccat ttatctaaca
    11581 atcgttttaa tgcctgccct ttagtcatgt ttggcataat aatatcaatg ctatcgtgcc
    11641 cacttgagac aagtttaata tcattgctga attgcgttgc tacttcttcg tcaacatttg
    11701 gatgcgtctc acgattaata ttaaatgcta ttttgacata atcatcctca ggtaattgtt
    11761 gtagactgtc aatttctttt aactgatgat aataaaatct cgtatcttct ttaaatgctt
    11821 cagaagtatg ttttaaaata tacgcgcttt tcaaaccaca gatgacgagt tgatcaatac
    11881 tttggttcat atttaagtaa tcgacgacct gttgaaacac ctgacgatta aagcttttat
    11941 aattatataa ctcattgcct ttataaataa ctgcaccatt ttcagaaata aaatacatat
    12001 ctctatcccc gaaaatagac ttcaacttcg catattgatt gccactcgca gcaataaatg
    12061 taatatctct atttctaagt tcagtaaaaa tcgcttcaaa tctcggttta tcgtatgtct
    12121 ttttcgaatc taaaaacgtc ccgtccatat ctaccgcaat agccttaacc atgattatcc
    12181 tcctcattgc tcttttagtc gtcttcatta tatccatctc atgacaagca atgaagtttg
    12241 tcataccaca atgaaactat attcacattt tgaaattgct ttcatatatg caaaacacat
    12301 tacactggat atagcattcg gaggcgaaca taaatttcag aatattgtaa aaatataacg
    12361 atatatttat catttggaca aaatatagtg tgatatacta atttataaca tctatacata
    12421 atgtgaaaaa atttttaaaa tacaattaaa tatcggtgta ataaatccct ttatacatag
    12481 gagttgtata catgaaacta aataactatt ctttaaaagt taaaaacaaa caactagttg
    12541 acaattgtga tttaaatttc tatcttggtc agatcaatca cattattggt aaaaatggtg
    12601 taggaaaatc tttattagct aaagatttct tactaaataa tagtggaaat atccctaagt
    12661 ccatttctca aaatgtaacc ttaatatcaa gttcatcaaa tattcctaat gatataacaa
    12721 aagatttttt attatcattg ttaaaatcaa aatttgaaaa caatcgacaa acattcgata
    12781 agatttataa catactaaac atcgaagcaa taccgtctaa cgtactacta aaaaacttga
    12841 gtgatggtca aaaacaaaag cttaaattat taagtttctt attggaagat catgatttaa
    12901 tcatattaga tgaagttaca aacgctttag acaagaaaac agttaatgaa atttatgaat
    12961 ttttaaatga ttttattcaa agccatcaaa ctaaaactat tatcaatatt acacataatt
    13021 tatccgattt aagtgctttg ccgggtaaat actttatttt taaagacctt caaatagaag
    13081 agtaccaatc aaaagaagaa gtcataaatg attacattaa tttataattg aggtgtccac
    13141 tttgaaatta gaatttaaaa aaagtatttc caataaaatt atttacacgc ttggtgtgct
    13201 attcatattc cttttcttac ttgggtattt tttaccaata ggaatagaca aagttaaaaa
    13261 tttaagctat ggtcagtttt tctttagttc ttatacagtt gccactgaat ttggcttttt
    13321 attattttca tttgttattg catattttat taataa
//
LOCUS       DAOBBH010000061        13113 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.061, whole genome shotgun sequence.
ACCESSION   DAOBBH010000061 DAOBBH010000000
VERSION     DAOBBH010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13113
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.061"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..2291)
                     /gene="fnbA"
                     /locus_tag="P6L01_001268"
     CDS             complement(<1..2291)
                     /gene="fnbA"
                     /locus_tag="P6L01_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbA"
                     /protein_id="HDP3030345.1"
                     /translation="MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTT
                     VEENGNSATDNKTSETQTTATNVNHIEETQSYNATVTEQPSNATQVTTEEAPKAVQAP
                     QTAQPANVETVKEEEKPQVKETTQPQDNSGNQRQVDLTPKKVTQNQGTETQVEVAQPR
                     TASESKPRVTRSADVAEAKEASDVSEVKGTDVTSKVTVESGSIEAPQGNKVEPHAGQR
                     VVLKYKLKFADGLKRGDYFDFTLSNNVNTYGVSTARKVPEIKNGSVVMATGEILGNGN
                     IRYTFTNEIEHKVEVTANLEINLFIDPKTVQSNGEQKITSKLNGEETEKTIPVVYNPG
                     VSNSYTNVNGSIETFNKESNKFTHIAYIKPMNGNQSNTVSVTGTLTEGSNLAGGQPTV
                     KVYEYLGKKDELPQSVYANTSDTNKFKDVTKEMNGKLSVQDNGSYSLNLDKLDKTYVI
                     HYTGEYLQGSDQVNFRTELYGYPERAYKSYYVYGGYRLTWDNGLVLYSNKADGNGKNG
                     QIIQDNDFEYKEDTAKGTMSGQYDAKQIIETEENQDNTPLDIDYHTAIDGEGGYVDGY
                     IETIEETDSSAIDIDYHTAVDSEAGHVGGYTESSEESNPIDFEESTHENSKHHADVVE
                     YEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTIEDTKEYTTESNLIELVDE
                     LPEEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIKSELGYEG
                     GQNSGNQSFEEDTEEDKPKYEQGG"
     gene            complement(2275..2377)
                     /locus_tag="P6L01_001269"
                     /pseudo
     CDS             complement(2275..2377)
                     /locus_tag="P6L01_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501263.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2760..4118)
                     /locus_tag="P6L01_001270"
     CDS             complement(2760..4118)
                     /locus_tag="P6L01_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HDP3030346.1"
                     /translation="MFNEIWPLISVVLGIVILLVLIIGFKLNTFISLIITSMITALML
                     GIPLTKIMETIEKGMGSTLGHIALIFGLGAILGKLLADGGGATRIADTLIQKFGQKHV
                     QWAMLVAAFIVGIALFFEVGLVLLIPLVFTVAKRANVSVLKLGLPMVTALSVTHGFLP
                     PHPGPVVIAKELKANVGDVLLYGMIIAIPVTLIAGPIFNKVAQKMIPSAYTREGDISA
                     LGAQKEFTDQEMPGFGMSLLTATLPVILMLVSTITQLVTGHDKPTNLFESIIYMIGTA
                     GTAMLIAVLFAIVTMGLMRKRKMNHIMESVTNAIYPIGMMLLIIGGGGTFKQVLIDGG
                     VGNTIAKMFEGTEMSPILLAWIVAAVLRIALGSATVAAISTTGIVLPLLQSSDVNVAL
                     VVLAIGAGSVILSHVNDAGFWMFKEYFGLTVKETFLTWSLLETIISVSGIIFILFISL
                     FV"
     gene            complement(4235..5788)
                     /gene="gntK"
                     /locus_tag="P6L01_001271"
     CDS             complement(4235..5788)
                     /gene="gntK"
                     /locus_tag="P6L01_001271"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDP3030347.1"
                     /translation="MKYMIGVDIGTTSTKSVLYDENGAFIMKHQIGYDLHTPNVDVSE
                     ENPDELFDAVLMTIKYIMRESKVNQDDIKFVSFSAQMHSLIAMDQQHQRLTNNITWAD
                     NRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLVKIFWMKHEWQDVFQRTAKFADIKT
                     YIFYHLFDTYIIDYSMASATGMFNLETLDWDVGALELLGISKEMFPELVPTTYVMKGM
                     KERYATLMGLNEDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTD
                     YKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIA
                     KRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYT
                     VYLALIEVMNETPNMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGL
                     KAVGDIEDFSIVSSMVGATNNHTPFEENVAVYQELVSIFINLSRSLTENYEQIADFQR
                     QHMAENKTQ"
     gene            complement(5812..6492)
                     /locus_tag="P6L01_001272"
     CDS             complement(5812..6492)
                     /locus_tag="P6L01_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDP3030348.1"
                     /translation="MMYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMA
                     KQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRV
                     KNQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHL
                     KPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGK
                     DIEGFWLN"
     gene            complement(6645..7409)
                     /locus_tag="P6L01_001273"
     CDS             complement(6645..7409)
                     /locus_tag="P6L01_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDP3030349.1"
                     /translation="MSNYSTGELAKLCNVTTRTIQYYDRKGILKPQGFTEGKRRVYTE
                     QQRQTLELILLLKDLGCALSDIDMLLKGEGTLKTLNTLLTMKQQEINQQVKQQQAVLN
                     KIKNVQYYVNEASTSPITHLKDIEHVMSKSAEMKSIRRNIWISAGIIGIIQYSSIMSS
                     ILMKNKWPFLIALPFMNCYGIGVTFYYQQKVAYLCPNCQHIFSPSLWAVIKAKHTATT
                     RRFECPNCHETHYCIEVPKAHMSTEQLEISHIQHNN"
     gene            complement(7574..8266)
                     /locus_tag="P6L01_001274"
     CDS             complement(7574..8266)
                     /locus_tag="P6L01_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDP3030350.1"
                     /translation="MYVDRKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHI
                     YSSALKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAK
                     EHILDIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSI
                     PVFLAERVEVLPVEIQIRTIGMDMWASLEHKIRYKNNAETEKYRDLLKECATEITEVE
                     DKLQQIHSEITE"
     gene            complement(8497..8913)
                     /locus_tag="P6L01_001275"
     CDS             complement(8497..8913)
                     /locus_tag="P6L01_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDP3030351.1"
                     /translation="MPFTMKGYPEKWKEFEVIKRQKAIEIANAMLKEGYLEKDVVPIA
                     AKKAKDWYRALSKEEIKALEEENFVQFHTATMEKAEVDSEYATTDNEEHIQEQSNQDK
                     SYFDSESIGNQNGGVRQREPLMDGAHPKVVESFKDV"
     gene            complement(9052..10875)
                     /locus_tag="P6L01_001276"
     CDS             complement(9052..10875)
                     /locus_tag="P6L01_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDP3030352.1"
                     /translation="MIIPAIIGYLCGNFQFGLLVATGTLAHIYVFKGPSRSKLRTVII
                     CNLAFAICMMLGTLTAKTPLVFGMTLLIVTVIPFYIFTALKIAGPSSTFFIVTFSLPI
                     NLPIAPEEALYRGFAILVGGILATMMVLITIVFSKNKAEEQAIQNDFKLISKLLHTYN
                     DKAAFLQVAKTAVDSFKASDKLLITSTSSNDKLSRRFQKLLLLHTSAQGIYSELLELN
                     AKQIRPLPDELIEMMDHIIAQLDNSDENVRYWRKEVTVTEEFQNLFNHILKIDEMVHA
                     NEARIAYEADMRKPLYSKRIYQNLTLDSIVFRNTLRYTAIMMIAIFIALMFDFEKAYW
                     IPLSAHTILLGTSTIHAIERGMARGLGTILGVLVLSVILLFSIPTPVAVILMGIAALF
                     TEALVGANYAIAVVFITIQVILMNGLASQNLTINIAFPRVIDVAIGIVIAIIGLFVLG
                     QRTASVLLPNVMAEVVRKEATLFHYLFSENQYKDNVYQKNTAMNLSVKLNNMTQVYNA
                     ANGELFSDKTLIQNYYPSLFALEEISFMLNRAMANEDRLTINEQLMGEYLATFENIAK
                     HLELNTELEIKILPDLPQYNYIQSAMMNIQHNGFRERDKNV"
     gene            complement(11352..12629)
                     /locus_tag="P6L01_001277"
     CDS             complement(11352..12629)
                     /locus_tag="P6L01_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDP3030353.1"
                     /translation="MKEKRTNVRWMFALAFFFIGVIAYMDRANISYIAKQMMDDLGMT
                     KPQFGLLASFFSLGYALMQVPSGMLAEKFGPRKMITIALVWWSAFTILTGMIKNHGLI
                     YLVRFLFGVGEAPMYPSNAVFNSFWFSKNEKGRASSALLAGSYFGPVLAPIVTIAIVN
                     AFNWQAVFYIFGAVGILMAVLWAIIAKDLPEQHRMVNEAEKRFIMENRDIVATEKSSP
                     PWNNFFKRFSFYAIAIQYFVVQFIITLFLIWLPTYLTEVFHVNFKEMSISSLPWLLMF
                     FLILSAGAISDRVLGLGRSKFVARGVIAIAGFIVFAVSIIFAVRTGNLYVSIFWLSLG
                     LGGIGISMGMSWAAATDLGRNFSGTVSGWMNLWGNIGALISPLLAGLFVEHLGWTMTF
                     QLLIVPAVIAVIMWFYVKPDQPLIVSDDKAIEK"
BASE COUNT         3978 a         2593 c         1893 g         4649 t
ORIGIN      
        1 ccaccttgtt catatttagg tttgtcttct tctgtgtctt cctcgaatga ctggttaccg
       61 ctattttggc caccttcgta acctaattca ctcttaatat caacgtggct attttcttcg
      121 atttcttcaa tcacgccata attaccgtga ccattttcag ttcctaaacc agaatgagaa
      181 atatgatgat tgttttcagt aatttcctcg attggtcctt gtgcttgacc atgttcttca
      241 ggtagttcat ctactagttc aatcagatta ctttcagtcg tatattcttt cgtatcttca
      301 attgttgtat ggtcgctcac tgcgccagtt acaatacctt ttgtagactc ttcgtcaaat
      361 tcaactaagt tagactcagt tgttacttgg ccaccacctg gatttgtatc ctcttcatat
      421 tcaacaacat cagcgtgatg ttttgaattt tcatgtgtcg attcttcaaa gtcaattgga
      481 tttgattcct cagaggactc agtgtatcct ccaacgtgac ccgcttcact atccacagca
      541 gtatggtaat cgatatcaat agctgatgaa tccgtttctt ctattgtttc aatataccca
      601 tcaacataac caccctcacc atctatagct gtgtggtaat caatgtcaag cggtgtattg
      661 tcttgatttt cttctgtttc aataatttgc ttggcatcgt actgcccgct catagttcct
      721 tttgcagtat cttctttata ttcaaaatca ttatcttgaa taatttgtcc atttttacca
      781 ttgccgtcag ctttattgct atataaaact aaaccattat cccaagttaa acgatatccc
      841 ccataaacat agtaagattt atatgctcgt tctggatacc catataattc agttctaaaa
      901 ttaacctgat ctgacccttg caaatattca cctgtataat gaatgacata cgttttatcc
      961 aacttatcta aattcaatga gtaactacca ttgtcttgca cactcaattt tccattcatt
     1021 tcctttgtta catctttgaa tttgttagta tctgatgtat ttgcataaac actttgtggc
     1081 aattcatctt ttttccctag atattcatat actttaacag taggttgtcc accagctaaa
     1141 ttactacctt cagtcaacgt ccctgttact gatacagtgt ttgactggtt tccattcatt
     1201 ggcttaatat aagctatatg tgtaaattta ttagattctt tattaaatgt ttcaattgat
     1261 ccatttacat ttgtataact attgctaaca cctggattat aaacaactgg tattgttttt
     1321 tctgtttctt caccatttaa tttagaagta atcttttgtt ctccattgct ttgtacagtt
     1381 ttagggtcaa taaataagtt gatttctaaa ttagctgtta cctctacctt gtgttcaatt
     1441 tcgttagtaa atgtatatct tatgttacca ttccctaaga tctcacctgt agccattaca
     1501 actgagccat ttttaatctc tggtaccttt ctagctgttg aaaccccata agtatttaca
     1561 ttatttgata atgtaaaatc aaaataatct cctcttttta atccatctgc gaatttcaat
     1621 ttgtatttca atacgacacg ttgaccagca tgtggctcta ctttatttcc ttgaggtgcc
     1681 tcaatagaac cactttctac tgtaacttta cttgtaacat ctgtgccttt aacttctgaa
     1741 acgtcactag cttccttagc ttccgctaca tctgctgatc ttgtcacacg tggcttactt
     1801 tctgatgccg ttcttggctg tgccacttca acttgtgttt ctgtcccttg attttgtgta
     1861 acctttttag gtgttaaatc tacttgtctt tgatttccgc tattgtcttg aggttgtgtc
     1921 gtttccttaa cttgaggttt ctcttcttct ttaactgttt ctacatttgc tggttgtgca
     1981 gtttgtggtg cttgtactgc ttttggtgct tcttcagttg ttacttgtgt tgcgtttgac
     2041 ggttgttctg ttactgttgc gttatatgat tgagtttctt ctatatgatt aacgttagta
     2101 gcagttgttt gtgtttcact tgttttatta tcagtagctg aattcccatt ttcttctact
     2161 gtagttgtct tttgttctga tgctgcagct tctttatctt gtcccatccc aacaacgatc
     2221 attgttccta agaatactga tgctgctccc aatttatgtt ttctaatgcc gtacctaaga
     2281 ttgtttttca ctataatatc tccctttaaa tgcaaaattc attaattttt ttaaacttaa
     2341 taaatgcaag tctatattgt tcggttttta aaagcaatac aaaaaagcga gttaatgaaa
     2401 agttaagatt gtcattaatt ttatgttaat tgataatttt attatttgaa atatacctat
     2461 aaattgtatt caagtcatca taaacccttg tcacacaagg cttgtatttt ttatacttat
     2521 ttttcaaatt aaattcatca ttatctaatt taaaacaata tactaagtgt ttcataatta
     2581 tcgcctgtac aatacgcaca aaaacatgtc ttgaaacgcc tttcattaca ctaaaatccc
     2641 caatatactt tttatatcgt tcggattctg actatttcag acgattttct gcataaaaat
     2701 aaacgtgttt caaggcaata tattgcaatt acctaaaaac acgtttactt aatatttagt
     2761 taaacaaata agctgatgaa tagaatgaag atgatacctg aaacggaaat aatcgtttct
     2821 aataatgacc atgttaagaa tgtttctttt acagttaaac caaaatattc tttaaacatc
     2881 caaaatcctg cgtcatttac atgagacaaa atcacactac ctgcacctat cgcaagtaca
     2941 actaatgcaa catttacatc tgatgattgt aataatggta agacaatacc tgtagttgaa
     3001 atcgcagcta ctgtagccga acctaatgcg atacgtagca cagctgcaac aatccatgct
     3061 agtaaaattg gagacatctc tgtaccttca aacattttag caattgtatt tccgacacca
     3121 ccgtcaatta atacttgttt aaatgtaccg ccaccgccaa taatcaataa catcattccg
     3181 attggataaa tcgcattcgt cactgattcc ataatatgat tcatcttacg ttttctcatt
     3241 aatcccatcg taacgattgc aaataatact gctattagca tggctgtccc tgctgttcct
     3301 atcatataaa tgatagattc aaatagattt gtaggtttgt cgtgcccagt tacaagttgc
     3361 gttatcgtag acactaacat taatatgact ggtaatgttg cagttaataa actcatacca
     3421 aatcctggca tctcttgatc cgtaaattct ttttgtgcac ctaacgctga aatatcgcct
     3481 tctcgtgtat acgcagacgg aatcattttt tgtgcaactt tgttaaatat aggtcctgca
     3541 atgagtgtaa ctggaatggc aataatcata ccgtacagta atacatctcc aacatttgcc
     3601 tttaattctt ttgcgatgac taccggtcct ggatgtggtg gtaaaaagcc atgtgtcaca
     3661 gataaagctg ttaccatagg tagtcctagt tttaacactg aaacatttgc gcgttttgct
     3721 actgtaaata ctaatggaat cagtaagact aaacctactt caaagaacaa tgcaataccg
     3781 acgataaatg ctgcaacaag cattgcccat tgtacatgtt tttgaccaaa tttttgaatc
     3841 aacgtgtctg caattcgagt tgcaccacca ccatcagcaa gtaatttccc aagtatggca
     3901 cctaaaccga atattagtgc aatgtggccg agcgtactgc ccatcccttt ctcaatcgtc
     3961 tccataattt tagtcaatgg tatacctagc attaacgctg taatcatcga tgtgataatt
     4021 aatgaaataa atgtatttaa tttaaaccca ataattaata ctaataaaat aacgatacct
     4081 aaaacaacac tgattaacgg ccatatttcg ttaaacatga cattcccctc tttctctttt
     4141 caatagaatg taacaccgtc gtcgcgcacg tattccagat aatatatagt agtcatggca
     4201 caacaacttt atctaagatg cttgagtgac gtatttattg tgttttattt tcagccatat
     4261 gttggcgttg aaaatctgca atttgttcat aattctctgt taaagaacga cttaaattga
     4321 taaaaatgga tacgagctct tggtaaacag cgacattttc ttcaaacggc gtatgattat
     4381 ttgtagcacc gaccatcgat gaaacgattg aaaaatcttc aatgtcacct acagctttaa
     4441 gtccaagcac gcaggcacct aagcatgaac tttcataact ttcaggaacc actaactctg
     4501 tgtcaaatat atctgacatc atttgacgcc atacttcact tttcgcaaaa ccacctgttg
     4561 cttttatcat attaggcgtt tcattcatta cttcaatgag tgcaaggtag acggtataca
     4621 aattgtaaag aacaccttct aatgcagcgc gaatcatatg ttctttttta tgagataaag
     4681 ttaaaccgaa gaatgaacct cttgcatttg cgttccaaag cggcgcacgt tctccagcta
     4741 aatagggatg gaatattaaa ccatctgcac ctggtttaac acgctttgca atttgagtta
     4801 agacatcata aggatcaaca ccgagacgtt tcgcagtttc gacttcactc gctagcaact
     4861 cgtcgcgcaa ccatctcaat acgacaccac cattatttac aggacctccg atgacgtagt
     4921 ggtcctctgt taagacataa caaaatattc tacctttgta atcagtacgt ggtttatcta
     4981 tcacagtacg aatcgcgcca gatgtaccga ttgtgacagc aacttctcct ttaccaacac
     5041 tattgacacc caaattagaa aggaccccat cactcgcacc aataacaaac ggtgtatctt
     5101 cattaagccc cattaatgtt gcataacgtt ctttcatgcc tttcattacg tacgttgttg
     5161 gcactaattc cgggaacatt tccttggaaa taccaagcaa ttctaatgcc ccaacatccc
     5221 aatctaatgt ttctaaatta aacatccctg ttgcggaagc cattgaataa tcaatgatat
     5281 atgtatcaaa taaatgatag aaaatgtatg ttttaatatc tgcaaactta gcagtacgtt
     5341 gaaatacatc ttgccattca tgtttcatcc aaaaaatttt cactaaaggc gacataggat
     5401 gaataggcgt acctgttcgc tgataaatcg cattgccatc atgcacttca tttattactg
     5461 ttgcatattt tgcagcgcgg ttatctgccc aagtaatatt atttgttaat ctttgatgtt
     5521 gctgatccat cgcaatcaag ctatgcattt gcgcactaaa tgacacaaat ttaatatcat
     5581 cttgattaac tttcgattcc ctcattatgt atttaattgt cattaatacc gcatcaaata
     5641 attcatctgg gttttcttct gagacatcaa cgtttggtgt gtgtaaatca tagccgattt
     5701 gatgtttcat gataaaagct ccattttcat catataagac tgacttggta ctcgtcgttc
     5761 caatgtcgac accaatcata tatttcatga taaatccttc tttctttcat tttaattcaa
     5821 ccaaaatcct tcaatatctt taccaacatc gtcaaaattt aaatgaaacg cttccttcaa
     5881 aatttgactg tcgtattgtt ccactgcatc aataaacact tgatgattat gatgtatgcg
     5941 ttcaaaatct tgcgggttct gttgcatacg ttgtcgcatg gatgttaaaa cgagtgccat
     6001 cattactggt tttaaatgac tccaaaatga gtttaaatat tggtggttag atgctttgat
     6061 taatgtttca tgaaattcaa agtcatgctt cgtaaatgat tctgcatctt caaattttac
     6121 tgccactttc atcatttcaa gttgtttctt catttctttt acgataggta gtctctcttg
     6181 atttttaact cttgaaaatg caaatgactc tagcatcagt cgcaaatcgt acatttcttt
     6241 cttttcttgt tccccaaacg gcaacacatg tgcacccatt ctttctaatt ggatgagttg
     6301 attttgttgc aataatttaa atgcatctcg aattggcgaa cgactcacat taaattgctt
     6361 tgccatttga ttttcagtga gtaacgtacc ttcagctatg tgaccattca caatacctaa
     6421 gcgtaattct gccgcgatac cttctccagt tgtcatacct tccaaccatt tctctggata
     6481 tccatacatc atcaaagcac tccttcatta catgacatac ttgtatacaa gtatgttaat
     6541 atagttctta agtgtttgca agcgctttct ttatgcgaac taaaatagtg accacccctc
     6601 ttcgatgtaa atttaaaggg aatgatcact atcacacgaa tgatttaatt gttatgttgt
     6661 atgtgggata tttctaattg ttctgtactc atatgcgctt taggtacttc aatgcaataa
     6721 tgcgtttcat gacagttcgg acattcgaat cgacgtgttg tcgctgtatg tttcgctttg
     6781 ataactgccc acaaagatgg tgagaatata tgctggcagt taggacataa ataggcaacc
     6841 ttttgttggt aataaaaagt aacaccaatg ccgtaacaat tcataaatgg taaagcaatt
     6901 aaaaacggcc atttgttttt catcaagatt gaactcataa tgctagaata ttgaattatt
     6961 cctataatac cagcactaat ccaaatgtta cgacgaatac ttttcatttc agctgattta
     7021 ctcatgacat gctctatgtc ttttaagtgt gtgattggag acgtcgacgc ttcatttacg
     7081 taatattgaa catttttaat tttgtttaat accgcttgtt gctgtttgac ttgttggtta
     7141 atttcttgtt gtttcatggt tagtaaggta ttgagtgtct tcaaagtacc ttcacctttt
     7201 agcaacatat ctatatcgct taacgcacaa cctaaatctt taagcaataa gattaactct
     7261 aatgtttgtc gctgttgttc tgtatacaca cgacgctttc cttctgtaaa tccttgtggt
     7321 ttcaaaatac ctttgcgatc ataatattga atcgttcgtg ttgtcacatt gcataatttt
     7381 gcgagttctc cagtcgaata gttagacata gattccacct cctacaatca ccatagttga
     7441 tgacccgacg tcacgagcaa gtacaatttc catattttta agaaatttat tatacttggc
     7501 gtcttatttt tatgatttcg taccatgttg atttacaaaa tcactcaaat taagcaacac
     7561 tcctactaaa cgtctactct gttatttcag aatgaatttg ttgtaattta tcttcaactt
     7621 cagtaatctc tgtcgcacat tctttcagta aatctcgata cttttccgtc tctgcattgt
     7681 ttttataacg tattttatgt tctaaacttg cccacatatc catacctatc gttctaattt
     7741 gaatttcaac aggcaatacc tcaacgcgtt ctgctaaaaa gactggaatg gatacaacga
     7801 tatgtaaact gcggtaacca ttttctttag ggtgctgaat ataatcttta cgttttatca
     7861 attgtacgtc ttcttgttta agcaacatct cttcaatcaa ataaatatca tctaagtaat
     7921 tacatactac gcgaattccc gcaatatcca gtatgtgttc tttggcagtt tctgcactaa
     7981 tctgtaatcc tttacgatta agcttttcta ttaaactacg catttctttc acacgtcgct
     8041 ccatatgatg tataggattg tgtttataaa tgtgattgaa attgtcatct aaaatactca
     8101 gtttagtgct tatttccttt agtgctgacg aataaatatg atctaactct acgaaaccta
     8161 ataacgtatc aaatgcttca tcaccatttt caaatttact taaactattt ttaaaatcat
     8221 gtcgcaaatc ctctaaatat agtgatggtt ttcgatctac atacattttt atactaacct
     8281 ccgatatata atcatcttta ttgtacctaa cattttatta agataatact aatacactat
     8341 cgaactttgg gctgatactg aaacagcaca aagaaactca aacaatccga attatgtatc
     8401 aaaaccttca attacaggaa gtaattttaa aatgatttaa cgcaaaaaaa cacctgttac
     8461 cgttatatag gtacaagtgc ttaattgata gagatgttat acgtctttaa atgattccac
     8521 aacttttgga tgtgcaccat ccataagcgg ttctctttgt cgaacgccac cattttggtt
     8581 gccaattgat tcgctatcaa agtacgattt atcttgattt gattgttctt gaatatgttc
     8641 ttcattatca gttgttgcat attcactatc cacctctgct ttttccattg ttgctgtatg
     8701 gaattgtacg aagttctctt cttctaatgc tttgatttct tctttcgata acgctcgata
     8761 ccaatctttc gcttttttcg ccgcaatagg aacaacatct ttttcaagat atccttcttt
     8821 taacattgca ttcgcaattt ctatcgcttt ttgccgttta attacttcaa attctttcca
     8881 tttttcgggg taacctttca ttgtaaaggg catttcctta acctccaata tgttataatt
     8941 catgttatat accaccataa ttataaaatg aaacatatta aacacaattt tttagactcc
     9001 cgtcataata acttcagaca aacgttagtc tgacctgata aatattttat tttaaacgtt
     9061 cttgtctctc tcacgaaaac cattgtgttg aatattcatc attgctgatt gaatataatt
     9121 atactgtggt aaatctggta atatctttat ttctaattct gtatttaatt ctaaatgctt
     9181 agcgatgttt tcaaatgttg ctaaatactc gcccattaat tgttcattta ttgtaagtct
     9241 atcttcatta gccatggctc tatttaacat aaagctaatt tcttctaatg cgaataagct
     9301 aggataataa ttttgaatca atgtcttatc actaaataat tcaccattag ctgcattata
     9361 aacttgcgtc atgttattta atttcacaga taggttcatg gccgtgtttt tctgatacac
     9421 attatcttta tattggtttt cagaaaacaa ataatggaag agcgttgctt ctttacgaac
     9481 aacttcagcc attacattag gaagcaatac ggatgcggta cgttgtccaa ggacaaataa
     9541 acctatgatt gcaatcacaa tacctattgc aacgtcaata actcttggaa acgcaatgtt
     9601 aattgttaaa ttctgtgatg ctaatccatt cattaaaata acttgtattg taataaaaac
     9661 tactgcaatc gcataatttg ctcccaccaa tgcttcagta aacaatgctg caatgcccat
     9721 taaaattact gcaacaggtg ttggtattga aaacaacaat atgactgaaa gtactaacac
     9781 acctaaaata gtacctaaac ctcgtgccat acctctctcg attgcatgta tagttgatgt
     9841 tcctagtaat attgtatgtg cagataacgg tatccagtat gctttttcaa aatcaaacat
     9901 taacgcaata aatatcgcta tcatcataat cgctgtatat ctcaatgtat ttctaaaaac
     9961 aatagagtct aatgttaaat tttgataaat gcgtttacta tataaaggtt ttcgcatgtc
    10021 tgcttcatac gcaatacgcg cttcatttgc atgcaccatt tcatcaattt tcaatatatg
    10081 gttgaataaa ttttgaaatt cctctgttac tgtcacttct tttcgccaat atcttacatt
    10141 ttcatcacta ttatctagtt gtgcaatgat atgatccatc atttcaatta actcatctgg
    10201 caatggtcga atttgtttag cgttcaactc taacagttca gaataaatcc cttgggcaga
    10261 tgtgtgtaat aataataatt tttggaaacg tctacttaat ttatcgttac ttgaagtaga
    10321 agtgattaat aatttatcag atgctttaaa actatccacc gctgtttttg ctacttgtaa
    10381 aaaagcagct ttatcattat aagtgtgtaa caacttagat atgagtttaa aatcattttg
    10441 aattgcttgt tcttcagctt tgtttttaga aaatacgatc gtgattaaca ccatcatagt
    10501 ggcaagtata ccgcctacta aaatcgcaaa gcctctatat aatgcttctt cgggagctat
    10561 aggtaagtta atgggtagac tgaatgtcac aatgaagaat gtcgatgacg gtccagctat
    10621 ttttaaggca gtaaatatat aaaatggtat aaccgtaaca attaataatg tcattccaaa
    10681 aacgagtggc gttttggctg ttagcgtacc aagcatcata catattgcaa acgctaaatt
    10741 acaaattatt acagttcgca gcttagatcg cgacggacct ttaaaaacat aaatatgggc
    10801 tagtgttccg gttgcaacta ataatccaaa ttggaaatta ccacataagt aaccgattat
    10861 tgcaggaata atcattaaca gtccctgtct tacaccttta taaaagtcta agttaagtcg
    10921 attgattttt ataagtgatg tcaaaaagat gtcattttga cttctccttt ccttgtcaaa
    10981 atatttccat aatcttttta ttagcatagt taaaatgaat ttgttgctat aaattttaat
    11041 tacgcaacat atgtgattat aacaccttat gctcaattat ctaaatttaa aaacttttaa
    11101 agtccaatta aaattaattt ctaatgttat ttaacatcgt tgaacctttg gtcgcccacc
    11161 tttgggctag ttaaaattta tatgtacttt tacaagcgtt acctgactaa tgttttaaaa
    11221 ctttatttct ttacatatat cattttaata acttcccgtt catcttaaac catatctgtt
    11281 ttaaaaagtt tcaatttcaa cgtaaaaaag caataagccc caacaatgtg aaagcttact
    11341 gcttgtttaa attatttttc tattgcttta tcatcactaa caattaaagg ttgatctggt
    11401 ttcacataga accacataat cacagcgatt actgctggaa cgattaacaa ttggaatgtc
    11461 attgtccaac ctaaatgttc tacgaataga cctgctaata gcggactgat taatgcacct
    11521 atattacccc ataagttcat ccaccctgat actgtaccag agaagttacg tcctaagtca
    11581 gttgctgcag cccaactcat acccattgaa ataccgatac caccaagacc tagtgataac
    11641 cagaaaatac ttacatataa atttcctgta cgtacagcaa agataattga aactgcaaac
    11701 acaataaatc ctgcgattgc aattacacct ctagctacga attttgaacg acctaatcct
    11761 aatacacggt cagaaattgc acctgctgat aagattaaga agaacattaa taaccaaggt
    11821 aatgaactaa tgctcatttc tttaaagtta acgtggaata cttctgttaa atacgtcggt
    11881 aaccaaatta agaataatgt aatgataaat tgtacaacaa agtattgaat tgcaattgca
    11941 tagaagctaa aacgtttaaa gaaattattc catggtggtg atgacttttc agtagctacg
    12001 atatcacgat tttccataat gaaacgtttc tccgcttcat taaccattct atgttgctca
    12061 ggtaagtctt tggcaataat cgcccataat acagccatta aaatacctac tgcaccaaaa
    12121 atgtaaaata ctgcttgcca gttaaatgcg ttaacaatag caattgtaac tattggtgct
    12181 aatacaggtc cgaaatatga tcctgctaat aatgcacttg atgctctacc tttttcattt
    12241 ttagagaacc aaaatgagtt aaacacagca ttagaaggat acattggcgc ctcacccaca
    12301 ccaaataaga atctcactaa ataaattaaa ccgtggttct taatcatacc cgttaagatt
    12361 gtaaatgcac tccaccaaac taatgcaatt gtaatcatct tacgtggacc gaatttttca
    12421 gccaacatcc ccgatggtac ttgcattaat gcataaccta gagagaagaa tgatgccaat
    12481 aaaccaaatt gtggtttggt catccctaaa tcatccatca tttgtttagc gatatatgaa
    12541 atgtttgctc tatccatata cgcaataacc ccaataaaga agaacgcaag cgcgaacatc
    12601 caccttacgt tagttcgttt ttccttcata actattccta cttccctaat taatttttta
    12661 aaacttaccc tgaaacgtta aaattttcgg acatctggca tgtccacccc tactttgtca
    12721 caaaattgta ccaaagttat tttttataat attatcagac atggaaagcg tttacaatag
    12781 aatctataaa aattttatta aattccgctt aacttttgct attaaaacga tataaaggaa
    12841 cttttagtta catagtagcc tatcatttgt taattaaaaa tactgcaatc ctagtgtttt
    12901 aaaacgtata ttgcaggctt tcttacatat tcgacattca ttttaaattg cataaaacta
    12961 cattgctcat tcattgtgca gcgcctgtta tttagatgat catttgttga cttttcgtac
    13021 ttatagcaag caattttttg atttattgct tataatacat tttaagacaa atgcttgttt
    13081 caacatcgca tacgacatca ttcattattc att
//
LOCUS       DAOBBH010000062        12963 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.062, whole genome shotgun sequence.
ACCESSION   DAOBBH010000062 DAOBBH010000000
VERSION     DAOBBH010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12963
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.062"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            21..479
                     /locus_tag="P6L01_001278"
                     /pseudo
     CDS             21..479
                     /locus_tag="P6L01_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            466..768
                     /locus_tag="P6L01_001279"
     CDS             466..768
                     /locus_tag="P6L01_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030354.1"
                     /translation="MRTNNHITDLDAIELSSGWNYNSKLVTGKNALLSDSLLRDIKVL
                     SSNAKGDADILETELRAVINAMSQNEGKSVDALKNSTTSLKNNLTKLKEQLDKTTH"
     gene            922..1281
                     /locus_tag="P6L01_001280"
     CDS             922..1281
                     /locus_tag="P6L01_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030355.1"
                     /translation="MILRYATNLKLAKELYQASKPKLQEDKGMIELFEHTFGLQKELV
                     KYVGIAEATTAALYSASFINKNISRLASLSTIGILSVAAYKHFEAGQGKKGAQHALDL
                     LGLATLSLLDTFSCNKK"
     gene            complement(1366..1977)
                     /locus_tag="P6L01_001281"
     CDS             complement(1366..1977)
                     /locus_tag="P6L01_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDP3030356.1"
                     /translation="MYTFDAQSLTARENYKLLIGSIIPRPIAFVTTLNQDASVNAAPF
                     SFFNIVNNHPPMVAIAVQRAGGKRKDTALNIERTGDFVVHITDEDNVQDINETAAPLA
                     YGDSELSRTGLSLLTSTTIKTPGIKDAKMRFECKLSQMILLGDVLDGADLIIGEIVTY
                     HIDDSIYEGDFKINPHALQAVSRLAGNDYAKLGGIFTIKRPEK"
     gene            complement(2056..2532)
                     /locus_tag="P6L01_001282"
     CDS             complement(2056..2532)
                     /locus_tag="P6L01_001282"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HDP3030357.1"
                     /translation="MVVVYVAGGCLWGVEAFFATIPGIIHTEAGRANGRSSKLDGPYD
                     GYAECVKLHFDDRMLTITDIMNYLFEIIDPYSVNQQGNDIGEKYRTGLYSCVDDHLIE
                     ARQFIERRKDRDQIAVEVLPLSNYIKSAEEHQQHLEKYPEDMHMCHISKDLLNKYK"
     gene            complement(2535..3035)
                     /locus_tag="P6L01_001283"
     CDS             complement(2535..3035)
                     /locus_tag="P6L01_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDP3030358.1"
                     /translation="MVLRKVIMQDLDQIIALENIGFSPEEAATREALKLRIEQIQETF
                     IVAEKNDEVIGYINGPVIKERYISDDLFKNVSINNSEGGYISVLGLVVAPNYQGQGIA
                     GRLLNYFETLAKNHHRHGVTLTCRESLISFYEKYGYRNEGVSESCHGGIKWYNLVKNI
                     KKDDEV"
     gene            complement(3270..4037)
                     /locus_tag="P6L01_001284"
     CDS             complement(3270..4037)
                     /locus_tag="P6L01_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDP3030359.1"
                     /translation="MIDIHNHILVDIDDGPKTIEKSIALLKQAKDEGVTSIVATPHHL
                     HPRYDNTFQQVLVKLAELRTHPEVQALDIKLFPGQEIRITDSILKGLDNGSIQGINRS
                     KYLLIEFPTGEVPHYTKQLFFEIQSRGYIPIIAHPERNRSIAKNPEILYELVANGALS
                     QLTSSSLVGGFGKNIQKLSLQFIECNLAHFVASDAHSCDQRPFLMQELFHNHKLKKYS
                     NDIEALLRNASSVINDNFVYLDRPTKPGKVKSFLKWF"
     gene            complement(4034..4726)
                     /locus_tag="P6L01_001285"
     CDS             complement(4034..4726)
                     /locus_tag="P6L01_001285"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HDP3030360.1"
                     /translation="MTNTRRSTSSLIVHEQPKSPISEKFRGIRSNIMFANPDSAVQSI
                     VITSEAPGAGKSTIAANLAVAYAQAGYKTLIVDGDMRKPTQHYIFNLPNNEGLSSLLL
                     NWSTYQDSIISTEIQDLDVLTSGPIPPNPSELITSRAFANLYDTLLMNYNFVIIDTPP
                     VNTVTDAQLFSKFTGNVVYVVNSENNNKDEVKKGKELIEATGAKLLGVVLNRMPKDKS
                     ASYYAYYGTDES"
     gene            complement(4743..5405)
                     /locus_tag="P6L01_001286"
     CDS             complement(4743..5405)
                     /locus_tag="P6L01_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapA"
                     /protein_id="HDP3030361.1"
                     /translation="MKEKFDLVKLLNILKKNIKLLLILPAICLVVSTALTFFVMPDKY
                     TASTQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAEL
                     NSFLKVTNQTNSQIITVSVTTGNKSESDKIVNRISKVFAHDMPKIMSVDNVTILSSAH
                     DNAVKVSPIVSVNLVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQ
                     KF"
     gene            complement(6254..6814)
                     /gene="icaR"
                     /locus_tag="P6L01_001287"
     CDS             complement(6254..6814)
                     /gene="icaR"
                     /locus_tag="P6L01_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ica operon transcriptional regulator IcaR"
                     /protein_id="HDP3030362.1"
                     /translation="MKDKIIDNAITLFSEKGYDGTTLDDIAKSVNIKKASLYYHFDSK
                     KSIYEQSVKCCFDYLNNIIMMNQNKSNYSIDALYQFLFEFIFDIEERYIRMYVQLSNT
                     PEEFSGNIYGQIQDLNQSLSKEIAKFYDESKIKMTKEDFQNLILLFLESWYLKASFSQ
                     KFGAVEESKSQFKDEVYSLLNIFLKK"
     gene            6978..8216
                     /gene="pgaC"
                     /locus_tag="P6L01_001288"
     CDS             6978..8216
                     /gene="pgaC"
                     /locus_tag="P6L01_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HDP3030363.1"
                     /translation="MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVD
                     ELEGITFLLACYNESETIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENND
                     FIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
                     TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGY
                     WDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLL
                     RDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFL
                     LSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKAL
                     KRKKGGYATWSSPDRGNTQR"
     gene            8180..8485
                     /gene="icaD"
                     /locus_tag="P6L01_001289"
     CDS             8180..8485
                     /gene="icaD"
                     /locus_tag="P6L01_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular adhesion protein D"
                     /protein_id="HDP3030364.1"
                     /translation="MVKPRQREYPTLKSSLNIVRETALIAISCVFWIYCLVVLLVYIG
                     TIFEIHDESINTIRVALNIENTEILDIFETMGIFAIIIFVFFTISILIQKWQRGRES"
     gene            8482..9354
                     /gene="icaB"
                     /locus_tag="P6L01_001290"
     CDS             8482..9354
                     /gene="icaB"
                     /locus_tag="P6L01_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HDP3030365.1"
                     /translation="MKYRKLIILVLSILIILPVSTLDGHHIANADDDSPKKLKYKENS
                     ALALNYHRVRKANFLNNFIYFFSSSKEIKNYSVSQSQFESQIKWLKSHDAKFLTLKEF
                     LYYKKKGKFPKRSVWINFDDMDETIYENAYPILKKYKIPATGFIITGHVGEENFHNLD
                     MISKKELKEMYKTGLWEFETHTHDLHNLSKNNKSKLMKASEATIIKDLNKSEKYLTKN
                     FKKSQKTIAYPYGLMNDDKLPVIKKAGLKYGFSLEEKAVTPNSNDYYIPRILISDDAF
                     EHLIKRWDGFHEKD"
     gene            9341..10393
                     /gene="icaC"
                     /locus_tag="P6L01_001291"
     CDS             9341..10393
                     /gene="icaC"
                     /locus_tag="P6L01_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide intercellular adhesin
                     biosynthesis/export protein IcaC"
                     /protein_id="HDP3030366.1"
                     /translation="MKKIRLELVYLRAIICAIIIITHLLTQITLKHENMEGGSLVLQF
                     YIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLTTRVKYILIPYILMGLFYSYSESLL
                     TDSSFNKQFIETVLLGQWYGYFIVVIMQFFILSYIIFKINYNLFNSKILLLLSFILQQ
                     SFLYYFTNNTAFHDTVLHYYPLSENTIIFGWIFYFFLGAYMGYNYERVLNFLERYLVI
                     MIVLAVATYFVFIALANGDYWNVTSFSYSLTPYNSIMFIVILGICTHFKTMLFNTIQM
                     ISAFSFFIYLLHPIILDSLFAYTNIFEDNTMVFLAISLLFILGLCIGVGMILREFYIF
                     RFIIGKQPYKLNINAY"
     gene            complement(10728..12770)
                     /gene="lip1"
                     /locus_tag="P6L01_001292"
     CDS             complement(10728..12770)
                     /gene="lip1"
                     /locus_tag="P6L01_001292"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip1"
                     /protein_id="HDP3030367.1"
                     /translation="MKSQNKYSIRKFSVGASSILIATLLFLSGGQAQAAEKQVNMGNS
                     QEDTVTAQSIGDQQTRENANYQRENGVDEQQHTENLTKNLHNDKTISEENHRKTDDLN
                     KDQLKDDKNSSLNNKNIQRDTTKNNNANPSDVNQGLEQAINDGKQSKVASQQQSKEVD
                     NSQDSNANNNLPSQSLTKEAPSLNKSDQTSQREIVNETEIEKVQPQQNNQANDKITNH
                     NFNNEQEVKPQKDEKTLSVSDLKNNQKSPVEPTKDNDKKNGLNLLKSSAVATLPNKGT
                     KELTAKAKDDQTNKVAKQGQYKNQDPIVLVHGFNGFTDDINPSVLAHYWGGNKMNIRQ
                     DLEENGYKAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGI
                     YKDWKPGQKVHLVGHSMGGQTIRQLEELLRNGNREEIEYQKKHGGEISPLFKGNNDNM
                     ISSITTLGTPHNGTHASDLAGNEALVRQIVFDIGKMFGNKNSRVDFGLAQWGLKQKPN
                     ESYIDYVKRVKQSNLWKSKDNGFYDLTREGATDLNRKTSLNPNIVYKTYTGEATHKAL
                     NSDRQKADLNMFFPFVITGNLIGKATEKEWRENDGLVSVISSQHPFNQAYTNATDKIQ
                     KGIWQVTPTKHDWDHVDFVGQDSSDTVRTREELQDFWHHLADDLVKTEKVTDTKQA"
BASE COUNT         4199 a         2199 c         1882 g         4683 t
ORIGIN      
        1 tgattagttt ataataataa agtgaaattg aaaaagacag ctattatgcg atgagcgaaa
       61 aacttcaagt aaaacaagaa tttaacggcg ttacaaggca atacttgaat ttcagaacag
      121 atagtatcga tgatctggat aagttcaaaa aagaattcaa acctgttatg cacttaaaag
      181 gtgatgcatt caatcaacag ttacaatctt tgattaacaa atatccacaa atacaaaaaa
      241 atatgaaatc agagttcatt gcttattatg ataaagaaaa aaatagagaa acagtaaaaa
      301 actatgcttg gaaccttcaa aaatctataa atgacattat gcaatcatat cctagcacaa
      361 aatttgtaca gttttataaa gatgatgttt ccccgtcaat ggtagatgga aatggccgtt
      421 taaaatcgga tactaatgta atttccatcg agggaggaaa gtatgatgag aacaaataat
      481 catataaccg atttagatgc tattgagtta agtagcggtt ggaattacaa ttcgaaattg
      541 gtgactggca aaaatgcact actatcagat agtcttttaa gagatattaa agtattaagt
      601 tctaatgcta aaggcgacgc tgacatacta gaaacagaac ttcgtgctgt aataaacgca
      661 atgtctcaaa acgaaggtaa atctgttgat gcacttaaaa atagtaccac ttctctaaaa
      721 aacaatctaa ccaaattaaa agagcaactc gacaaaacta cacattaatt tcatacattt
      781 tgagtgaaca gaattatatt tccatagcaa acattcctaa actacacata cgttacataa
      841 ttgattcatt tttatagaaa cgggtaaaaa tgataaagta atcatctttc acaaaaatcc
      901 aaagattggg aggaattaaa tatgattttg cgttacgcaa caaatttaaa acttgcgaaa
      961 gagctatacc aagccagtaa acctaaattg caagaagata agggtatgat tgaactgttt
     1021 gaacatactt ttggcttgca aaaagaactt gtaaaatacg tcggcattgc agaagcaact
     1081 actgctgctt tatactcagc tagctttatc aataaaaaca tttctcgcct tgcatcacta
     1141 tcaacgatag gcattttaag tgtagctgct tacaaacatt tcgaagctgg ccaaggtaaa
     1201 aaaggtgcac aacatgcatt agatttatta ggtcttgcta ctttaagttt actagataca
     1261 ttttcttgta ataaaaaata gtcttcattt tagaagcaca tactttagca cctgtgtact
     1321 tttgattcaa tccataaacg gtacaccgct atagcatgtg ttttattatt tttctggtct
     1381 ctttatcgta aatattcccc ctaatttagc ataatcattt ccggctaaac gagaaacagc
     1441 ctgtaatgcg tgtggattga ttttaaaatc accctcataa atactgtcat caatatgata
     1501 agttacaatt tcacctatta ttaaatcagc cccatctaat acatctccaa gcaatatcat
     1561 ttgcgatagt ttacattcga atctcatttt cgcgtcttta attcctggcg tcttaatcgt
     1621 tgtagatgtt aaaagtgata atcctgtacg actcaactca ctgtcaccat atgctaacgg
     1681 cgctgcagtc tcattaatat cttgaacatt atcttcgtct gtaatatgca caacaaagtc
     1741 tccagtccgt tctatattca aggcagtatc ttttctctta cctcctgcac gttgaactgc
     1801 aatagcaacc attggcggat ggttattaac aatattaaaa aagctaaatg gtgctgcatt
     1861 tactgatgca tcttgattta atgttgtaac aaatgctata ggtcgtggaa taattgaacc
     1921 aattaataat ttatagtttt ctctagcagt taatgattgt gcatcaaacg tatacataat
     1981 acctacctct tttcttagta tatctaggta tttctccgat tttggttaat ttaaacatct
     2041 attctcctct gaaaatcact tgtatttatt tagcaaatct tttgaaatat gacacatatg
     2101 catatcttct ggatattttt ctaaatgttg ctgatgttct tcagcacttt taatgtagtt
     2161 agacagcggt aagacttcca ctgcaatttg atctctgtct ttacgtcgtt caatgaactg
     2221 acgcgcttca attaagtggt catctacaca actatataaa cccgttcgat acttttctcc
     2281 aatatcattt ccttgttgat tcacactgta aggatcaatg atttcaaata aataattcat
     2341 aatgtctgta attgttaaca tacgatcatc gaaatgaagt ttgacacatt cagcataacc
     2401 atcatacgga ccgtctaatt tagagcttct tccatttgct cttcctgctt ctgtatgtat
     2461 aattccaggt attgttgcaa aaaatgcttc aacaccccat agacaccctc ctgctacata
     2521 aacaactacc atatttacac ctcatcatcc ttttttatat ttttaacaag gttataccat
     2581 ttaataccgc catgacatga ttctgataca ccttcattac gatacccata tttttcataa
     2641 aatgaaatta atgattctcg acatgttaac gttacaccat gtcgatgatg attcttagca
     2701 agagtttcaa aatagtttag taagcgacct gcaataccct gaccttgata atttggtgct
     2761 acaacaagac ctaacacact aatatagcca ccttcactat tatttatgga gacattttta
     2821 aataaatcat cgctaatgta acgctctttt atgactggac cgttgatgta tccaattact
     2881 tcatcattct tttcagctac aataaaagtt tcttgaattt gttcaattct caatttcaat
     2941 gcctctcgtg ttgcagcttc ttccggtgaa aaaccaatat tttcaagtgc aataatttga
     3001 tctaagtcct gcatgattac ttttcttaat accataaaaa cacttcctta gccctatata
     3061 tcaaaagtaa taccttcatc tttatattca ttattttcaa actaccttca ctataaaaaa
     3121 cgagtctttt tattgaaagc cattcgcctt aatcaattag acaagttgta ttatacgaat
     3181 tagtaaataa tcatgattat aattcatttt aagcatactc aaaaagactg gtacatgtat
     3241 accagtctaa ttcgaaaaaa tatatttagt taaaaccatt tcaaaaacga cttcactttt
     3301 cctggcttag tcggtctgtc taaataaaca aaattatcat tgattactga actggcattt
     3361 cgaagtaatg cttcaatatc attcgaatat ttcttcaatt tatgattgtg aaataattct
     3421 tgcatcaaaa atggtctttg gtcacatgaa tgtgcatctg aagctacaaa atgagccaaa
     3481 ttacattcta taaattgtaa tgataacttt tgaatgtttt taccaaatcc accaactaaa
     3541 gaactcgatg ttaattgact caatgcccca tttgcaacca attcatataa tatttctgga
     3601 tttttggcga tacttctatt tctttcagga tgtgcaatga ttggtatgta gcctctcgat
     3661 tgtatttcaa aaaacaattg ttttgtataa tgtggtactt cgcccgttgg aaattcaatt
     3721 aataaatatt tcgaacgatt aataccttga atactaccat tatctaagcc tttcagaatc
     3781 gaatctgtaa ttctaatttc ttgcccggga aataatttaa tatccaatgc ttgaacttct
     3841 ggatgcgttc ttaactccgc caatttcaca agcacttgtt gaaatgtatt atcatatctc
     3901 ggatgcaaat gatgaggtgt cgctacaata cttgttacac cttcatcctt agcttgcttt
     3961 aatagtgcaa tactcttttc aattgtttta ggaccatcat ctatatcaac taatatatgg
     4021 ttatgaatat caatcatgat tcatcagtcc cataatatgc atagtaacta gcacttttat
     4081 ctttaggcat tctatttaag actacaccta ataatttagc acctgttgct tcaataagtt
     4141 cttttccttt tttaacttca tctttattat tattttccga attaactacg tagacaacat
     4201 tgccggtaaa ctttgaaaat aattgcgcat ctgtaactgt gttcactggt ggcgtatcga
     4261 taatgacaaa gttataattc atcaataatg tgtcatacaa atttgcaaat gcccttgatg
     4321 taattaactc tgacggattc ggtgggattg gcccagacgt caagacgtct aaatcttgaa
     4381 tttcagttga gataatactg tcttgataag ttgaccaatt tagcaataaa cttgataggc
     4441 cttcattgtt tggcaaatta aaaatataat gctgcgtagg tttacgcata tccccgtcta
     4501 cgattagtgt tttataacct gcttgcgcat atgcaactgc taaatttgct gcaattgtag
     4561 acttacctgc gcctggtgcc tctgaagtga ttacaatgct ttgaactgca ctgtcaggat
     4621 ttgcaaacat aatatttgat cttatgcctc gaaatttctc gctaataggt gactttggtt
     4681 gttcatggac aattaaactt gatgtacttc ttcgtgtatt cgtcatggta attcctcgta
     4741 aattaaaatt tttgtattga acctaaaata ggtaatccta gttgcgattc aacatcttct
     4801 tctgtcttaa tacgcttatc taataattct tttaagaaaa taatcaatat tgctaaaaca
     4861 ataccaacaa taatgctgat cactaagttg acagatacta ttggagatac ttttacagca
     4921 ttatcatgtg ctgaggaaag tatcgtaaca ttatcaacac tcataatttt aggcatatca
     4981 tgtgcaaaaa ctttagatat tctattaaca attttgtcag attcagattt attcccagtt
     5041 gtaactgata cagtaataat ctgagagttt gtttgattgg ttacttttaa aaacgaattc
     5101 aactcagctg ttgaatactg accatcaaac tctctagata ctttatctag aattcttgga
     5161 cttttgataa tttccgtata tgtattaaca gactgcaaac tactttgaac attttggaaa
     5221 gctaaatcac ttgaggactt tttcatgttc actagtattt gagtagaagc agtatatttg
     5281 tcaggcataa caaagaaggt taatgccgta cttactacaa gacatattgc cggtaaaata
     5341 agcaataatt taatattctt ctttagaata tttaatagtt ttactaaatc aaacttttct
     5401 ttcatggttt cctccacata atcaatcatt gtattcatta tgtatgtttt ataaatcgga
     5461 caattatatc tagtttaacg accacaaaac atacacaact acattttctc taattattta
     5521 tataaatatt ttatcgttta aaattatatc atgattctct accgttatgt ataacttatt
     5581 tatatttttg tacaagatat aatattgtcc aacttcaaat atccaaacct attaataata
     5641 aaactagata ccatcgtact ctatcatggc tttcttataa tcgagtagaa gcatcatcat
     5701 tacttgatta tttgctcttt acaacaccga gcgtgcccgt actcggtaat tcaatacctt
     5761 gcctaacccg tcactgtgag ttgggttaat gataataaag cccacacctt ttaaaaagat
     5821 gtgggtaatt tatataattt ttatttacat ttttaactta taaaaaaaag cgcctatgtc
     5881 atgatttacc atcacatagg cgcttatcaa taaattatta cttattactt tccatttcat
     5941 ctaatttatg cggatttcct gtaattagat gacaacttat tcttttcagg ggaacattac
     6001 acttttataa tatgttcaaa gacaaactta accattcaca aatataaaga ataatattat
     6061 caaatcattg aacaaatcgt attttgcaac aattgatatt tatattaatg tattgcattt
     6121 aatttataaa attcatatac atcttaatat tctcaatatc gatttgtatt gtcaacttta
     6181 tatagattta aaaaaataat ctcatgtctt tttttacaaa agtaagttaa ttattacaaa
     6241 ctagtaacaa aaattatttc ttcaaaaata tatttagtag cgaatacact tcatctttga
     6301 attgactttt actttcttcc actgctccaa atttttgcga aaaggatgct ttcaaatacc
     6361 aactttcaag aaacagcaat attaaattct gaaagtcttc ttttgtcatc tttatctttg
     6421 attcatcata gaattttgct atctctttac ttaatgattg atttaaatct tgtatttgtc
     6481 cgtaaatatt tccagaaaat tcctcaggcg tattagataa ttgaacgtac attctaatat
     6541 acctttcttc gatgtcgaaa ataaactcaa ataagaattg atataaagca tcaattgaat
     6601 agttcgattt attttgattc atcataataa tattattaag gtaatcaaaa cagcatttaa
     6661 cactttgttc gtaaatactt tttttcgagt caaaatggta atataaactc gctttcttta
     6721 tatttacact tttagctata tcatcaagtg ttgtaccgtc ataccccttc tctgaaaata
     6781 aggttattgc gttatcaata atcttatcct tcaattttta taacccccta ctgaaaatta
     6841 atcacactat gttacaggaa aattaagttg caattacaaa tatttccgtt taattataac
     6901 aacaatctat tgcaaattaa aatactatca attaccatat ggcttacaac ctaactaacg
     6961 aaaggtaggt aaagaaattg caatttttta actttttgct tttttatcct gtatttatgt
     7021 ctatttactg gattgtcggt tcaatttatt tctattttac cagagaaatt agatattcat
     7081 tgaacaagaa gcctgacata aatgtggatg aattagaagg cattacattt ttacttgcct
     7141 gttataacga aagtgaaacg attgaagata cgttgtctaa tgttcttgca ctcaaatacg
     7201 agaagaaaga aattattatc attaatgatg gaagttcaga taatacagca gaactcatct
     7261 ataaaatcaa agaaaataat gactttattt tcgtcgattt acaagaaaac agaggtaaag
     7321 ccaacgcact caatcaaggc attaaacagg cttcatatga ttatgtaatg tgcttggatg
     7381 cagatactat cgttgatcaa gatgcaccat attatatgat tgagaatttc aaacatgatc
     7441 caaaacttgg tgcagttaca ggtaatccta gaattcgaaa taagagttct attttaggta
     7501 aaattcaaac gatagaatat gcaagtttaa ttggctgtat taagcgaagt cagacacttg
     7561 ctggcgcagt caatactatt tcgggtgtct tcactctatt taaaaaaagt gcagttgtcg
     7621 acgttggcta ctgggatact gatatgatta ccgaagatat tgcagtttct tggaaattgc
     7681 atttacgtgg atatcgtatt aagtatgaac cgcttgccat gtgttggatg ttggttccag
     7741 aaacattggg aggtctttgg aagcaacgcg tgagatgggc tcaaggggga cacgaagtat
     7801 tactacgaga cttttttagc acaatgaaaa cgaaaaggtt tcctttatat attttgatgt
     7861 ttgagcaaat catctcaatt ttatgggtat atatagtgct tctatattta ggctatttgt
     7921 tcataacagc aaacttctta gactatacat ttatgacata tagtttttca atatttctac
     7981 tatcatcatt tactatgact tttataaacg ttattcaatt tacagtcgca ctctttattg
     8041 atagtcgcta cgagaaaaag aatatggctg gactcatatt tgtaagttgg tatccgacag
     8101 tatactggat tattaacgca gcagtagttc ttgtcgcatt tccaaaagca ttaaaacgta
     8161 agaaaggtgg ttacgcaaca tggtcaagcc cagacagagg gaatacccaa cgctaaaatc
     8221 atcgctaaat attgtaagag aaacagcact tatcgctata tcgtgtgtct tttggatata
     8281 ttgtttagtt gttctactcg tttatattgg tactatattt gaaattcatg acgaaagtat
     8341 caatacaata cgtgttgctt taaacattga aaatactgaa attttagata tatttgaaac
     8401 tatgggcatt ttcgcgatta tcatttttgt attttttaca attagcatat tgattcaaaa
     8461 atggcagaga ggaagagaat cgtgaagtat agaaaattaa taattttagt gttgagtatc
     8521 ttgatcatat tgcctgtaag cacactggat ggtcatcata ttgcaaatgc agatgacgat
     8581 tcacctaaaa aactgaaata taaagaaaat agtgctctgg cattaaatta tcaccgtgtt
     8641 agaaaagcga attttctgaa taattttatt tacttctttt ctagtagtaa agaaattaaa
     8701 aattatagtg ttagtcaatc acaatttgaa tctcaaataa aatggctaaa atcacatgat
     8761 gctaaatttt taaccttgaa agaattttta tattacaaga aaaaaggtaa gtttccaaaa
     8821 cgaagtgtat ggattaactt tgatgatatg gatgaaacta tttatgaaaa tgcttatcca
     8881 atcttaaaaa aatataaaat accggcaact gggtttatta tcacaggtca tgttggggaa
     8941 gaaaactttc acaacctcga tatgattagt aaaaaagaac taaaagaaat gtataaaact
     9001 gggttatggg aatttgaaac acatacccac gatttgcata acttatctaa aaataataag
     9061 tcaaaattaa tgaaagcttc tgaagctaca atcataaaag atttaaacaa aagtgaaaaa
     9121 tatctaacta aaaactttaa aaagtcgcag aaaactatag cctatcctta tggcttgatg
     9181 aatgacgata aattaccggt aatcaaaaaa gctgggttaa aatacggttt ttcattagag
     9241 gaaaaagcag tcactccgaa ctccaatgat tattacatcc ctagaatatt aattagtgat
     9301 gatgcttttg agcatttaat taagagatgg gacggattcc atgaaaaaga ttagacttga
     9361 actcgtatat ttacgtgcta ttatatgtgc aattattatt atcacacatt tacttacaca
     9421 aattacttta aaacatgaaa atatggaggg aggatcctta gtattacaat tttacattcg
     9481 taatattgtg atttttggta caccttgctt tattatcttg tcacagttac tgacaacctt
     9541 gaattaccaa aaagtcacct atagatactt aactacacgc gtaaaatata tacttattcc
     9601 ttacatatta atgggattgt tttacagtta tagtgaatca ttattaacag attcaagttt
     9661 caataaacaa ttcattgaaa ctgtcctatt aggtcaatgg tatggctatt ttatcgttgt
     9721 tatcatgcaa ttctttattt tgagttatat catttttaaa attaactata acctattcaa
     9781 cagtaaaata ttattattgt tatcttttat tttacagcaa tcatttttat attactttac
     9841 gaacaacaca gcgtttcacg ataccgtgct acactattat ccattaagtg aaaacactat
     9901 aatattcgga tggatttttt atttcttttt aggtgcatat atgggttata actacgaacg
     9961 tgtattaaat ttcttagaac gttatttagt tattatgatt gtattagctg tagctactta
    10021 ttttgtgttt attgcgttag caaatggaga ctattggaac gttaccagct tttcatattc
    10081 attaacacca tataatagta ttatgtttat tgttatcttg ggtatttgca cgcattttaa
    10141 aacaatgtta tttaatacga ttcaaatgat tagtgctttc tcattcttta tttatttatt
    10201 acatccaatc attctagact cattgtttgc atatacaaat atatttgagg ataatacaat
    10261 ggtctttcta gcgatatcac tactattcat tttagggtta tgtataggtg tcggtatgat
    10321 attgcgtgaa ttctatatct ttaggtttat tattggaaaa caaccatata aattgaacat
    10381 taatgcttat taattattaa gctatgttaa aaacacgcgg tgggcgaaat cagtttgaat
    10441 tgactgactt cgttttaccg cgtgtttaat aatgttatac atatattcta attgcacatt
    10501 taaacttcgt aaatgccaat gggagtggga cagaaatgat attttcgcaa aatttatttc
    10561 gtcgtcccac cccaacttgc attgtttgta gaatttcttt tcgaaattct ttttgttggg
    10621 gccccgccaa gatattactt gaataattct tttagaattc taaataatga tccgattaat
    10681 tgaaagaagt ctgctgtcat tgtatatacc tccctttact ttataaatta tgcttgctta
    10741 gtatcagtca ccttttcagt tttcactaaa tcgtctgcta aatgatgcca aaaatcttgt
    10801 aattcttctc ttgtgcgcac tgtatcagaa ctgtcttgtc ctacaaagtc aacatgatcc
    10861 caatcatgtt ttgtaggcgt cacttgccaa atgccttttt gaattttatc cgtcgcattt
    10921 gtataagctt gattaaatgg atgttgagaa gaaataacgg atactaaacc atcgttttct
    10981 cgccattctt tttcagttgc tttaccgatt aagttaccag taatcacaaa tgggaaaaac
    11041 atatttaagt ctgctttttg tctatcgcta tttaatgctt tgtgcgttgc ttcaccagtg
    11101 tatgttttat acacaatgtt agggttcaac gacgttttac gatttaaatc tgttgcaccc
    11161 tcacgcgtca gatcgtaaaa tccattatct tttgatttcc ataaattaga ttgtttaacg
    11221 cgtttgacat aatcaatata tgattcattt ggcttctgtt ttagacccca ttgagccaac
    11281 ccgaagtcta ctcttgaatt tttattacca aacattttac cgatatcaaa tacgatttgt
    11341 ctcactaaag cttcattacc agctaaatct gatgcgtgtg taccattatg tggtgttcct
    11401 aaagtagtaa ttgatgaaat catgttgtca ttattacctt tgaatagtgg agaaatttcg
    11461 ccaccatgtt tcttttgata ctctatttct tcacgattac cattacgcag taattcttct
    11521 agttgacgta tcgtttgacc gcccatacta tgtccaacta ggtgtacctt ctgtcctggt
    11581 ttccagtctt tgtaaattcc ttcgtatgtt tttccataac gttcatgtcc atattttgct
    11641 gcatgtgctg caccataatc tacacgaccg cctttgatat aataataaag ttcaactgcg
    11701 cggtcatagt tacttccaaa agcacttata cttgcttcat aagctttgta accattttct
    11761 tctaaatctt ggcgaatgtt cattttatta ccgccccaat aatgagctaa cactgaagga
    11821 ttaatatcat ctgtaaaccc attgaaacca tgcactaaaa cgataggatc ttgattttta
    11881 tactgccctt gtttggcaac tttattcgtt tgatcatctt tcgcttttgc agtaagttcc
    11941 tttgtcccct tattcggtaa cgtggctact gcactacttt ttaataaatt taatccattt
    12001 ttcttgtcat tgtcttttgt tggttctact ggtgattttt gattgttttt taaatctgaa
    12061 actgatagtg ttttttcgtc tttttgaggt ttcacttctt gttcattgtt aaaattgtgg
    12121 ttagtaattt tatcattcgc ttgattattt tgttgtggtt gtactttctc tatttctgtc
    12181 tcattaacaa tttctcgttg acttgtttga tctgacttat ttaatgatgg tgcttctttt
    12241 gttaaacttt gtgaaggtag attgttatta gcgtttgaat cttgactatt atctacctct
    12301 tttgactgtt gctgtgacgc cactttactt tgtttgccat cattaatagc ctgttctaac
    12361 ccttgattta catcgctagg attagcattg ttattttttg ttgtatcacg ttgaatattt
    12421 ttattattaa gcgatgaatt tttatcatcc tttagttgat ctttattcaa atcatctgtt
    12481 ttacgatgat tttcttctga tattgtttta tcattatgca agttcttagt taaattttca
    12541 gtatgttgct gttcgtcaac accgttttca cgttgataat tagcattttc cctagtttgt
    12601 tgatccccaa tagattgtgc tgtaactgta tcctcctgtg aatttcccat attcacttgc
    12661 ttctcagctg cttgtgcttg tccaccactt aaaaatagta atgtagctat taaaatggaa
    12721 gatgcaccta cactaaattt acgaatacta tacttatttt gacttttcat cattgtcagc
    12781 acctctattt ttttagttta gataaacaaa ttgataatta ttcgaggaat aaactcaaat
    12841 aatttactta accaagcact cataatgacc atcctttcta ttgtttaatt tgacgagaaa
    12901 gcgtttacat ttgtatttaa aaaatatatt ctccaaagag aatttaaagt gtatacgcta
    12961 atc
//
LOCUS       DAOBBH010000063        12593 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.063, whole genome shotgun sequence.
ACCESSION   DAOBBH010000063 DAOBBH010000000
VERSION     DAOBBH010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12593
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.063"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            463..1524
                     /locus_tag="P6L01_001293"
     CDS             463..1524
                     /locus_tag="P6L01_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDP3030368.1"
                     /translation="MSNANNADNKQFFSKILNSIGAGVVIALVPNALLGEVLKIFKSG
                     NEILELTYQLVILIQSFMAFIIGVLAAHQFKFNGAGAAIVGTSAMIGSGAVVYSNNSF
                     MLKGIGDIINTSLVVIIACLIYMVLQNKLGSFELIILPVLVPIVSGGIGLITLPYIRK
                     ITQAIGNVIHSFTDLNPLLMSILISVAFSLLMVTPISLVAIATAISLNGLGSGAANLG
                     IVAACVTFLFGSLRVNSIGVNAVLLIGAAKMMIPVYLKNLIISIPLTINGIITGIIAY
                     VLQVKGTPLSAGFGYTGLVGPINAFNRMSGDPTMNIILLALGYFVIPFVSAFIVHELC
                     KKFIPIYSNDIYKFEVPKQ"
     gene            1782..3239
                     /locus_tag="P6L01_001294"
     CDS             1782..3239
                     /locus_tag="P6L01_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500488.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDP3030369.1"
                     /translation="MKCLIRFILVLGLLISSAMVYINPTAHAEQDQTWEKIKERGELR
                     VGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTG
                     KIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKR
                     TEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISN
                     VKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISK
                     YGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPML
                     VQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSL
                     GLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISF
                     DPFTPLLVAAALYFVLTFVLTRIMNMIEGRLNASD"
     gene            3232..3954
                     /locus_tag="P6L01_001295"
     CDS             3232..3954
                     /locus_tag="P6L01_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDP3030370.1"
                     /translation="MIKINNLNKVFGDNEVLKDINLEINQGEVVAIIGPSGSGKSTLL
                     RCMNLLEVPTKGQVIFEGNDLTEKGTQVDKLRQKMGMVFQNFNLFPHKKVVDNIILAP
                     KLLKKDNNDELHKEALSLLDKVGLKEKADVYPNQLSGGQKQRVAIARALAMHPDVILF
                     DEPTSALDPEVVGDVLKVMKDLAKEGMTMVVVTHEMGFAKDVSDKVIFMADGVVVESG
                     TPVEIFEQPQHERTQNFLARVL"
     gene            4105..5232
                     /gene="queG"
                     /locus_tag="P6L01_001296"
     CDS             4105..5232
                     /gene="queG"
                     /locus_tag="P6L01_001296"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDP3030371.1"
                     /translation="MDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANG
                     YASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWG
                     QDYHTIMRKRLDMLAAFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVIN
                     PKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFL
                     TQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLR
                     MSNKEFKQTYGHLAGAWRGKKPIQRNAILALAHFNEVDAIPELKKVATTDERPMIRAT
                     AYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE"
     gene            5237..5707
                     /gene="trmL"
                     /locus_tag="P6L01_001297"
     CDS             5237..5707
                     /gene="trmL"
                     /locus_tag="P6L01_001297"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDP3030372.1"
                     /translation="MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKM
                     LKRAGLDYWEFVNITYHDSIEAFFEATNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIF
                     GKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN"
     gene            5866..6465
                     /locus_tag="P6L01_001298"
     CDS             5866..6465
                     /locus_tag="P6L01_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HDP3030373.1"
                     /translation="MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAP
                     VLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGVPAGQVYPIAYEKDAIELIADKI
                     KTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
                     TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA"
     gene            6490..6642
                     /locus_tag="P6L01_001299"
     CDS             6490..6642
                     /locus_tag="P6L01_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030374.1"
                     /translation="MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKN
                     SEEDSQ"
     gene            7190..7585
                     /locus_tag="P6L01_001300"
     CDS             7190..7585
                     /locus_tag="P6L01_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030375.1"
                     /translation="MLGKYWIQLLIATVIISLISIKAFPLAIGALYLPVIFKVIKLQL
                     NLSKGLIDDVNAQTFIKSNQSGIVISVICCLLITGILYYTLDGFYASLTGVLGTLVAL
                     NPYTTIVSAVLYILTAIATVEATKTKYRN"
     gene            7781..9166
                     /gene="fumC"
                     /locus_tag="P6L01_001301"
     CDS             7781..9166
                     /gene="fumC"
                     /locus_tag="P6L01_001301"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDP3030376.1"
                     /translation="MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVV
                     YGFAQLKRAAALANFDLGKLSEAKKDAIVYACDQILSGELDEHFPLVVWQTGSGTQSN
                     MNVNEVVSYVANMYLKDHQIDESIHPNDDVNESQSSNDTFPTAMHVALYQEVETKLEP
                     ALKLLRNTLKEKEDKFESIIKIGRTHLQDATPIKLGQEISGWRYMLDRCEIMLSESKK
                     HILNLAIGGTAVGTGINAHPEFGDKVAHYISENTGYPFVSSENKFHALTAHDEVVQLH
                     GTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMV
                     AVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPI
                     EENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEA
                     WIKPEDMVDPH"
     gene            complement(9619..10440)
                     /locus_tag="P6L01_001302"
     CDS             complement(9619..10440)
                     /locus_tag="P6L01_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDP3030377.1"
                     /translation="MKFKIPENFNDLSLRDIFQQLKVPKKDLHHLNMSKDITINDKPA
                     RLMDKVHTGDDVFVPTIDEKSNYVPSYRYAQIKYEDDDMAIVMKPKGVKTHPNDLKES
                     NTLMNHVIYTIDSDYVEPIHRLDQETVGLLIVAKNPLMKKILDRMLEDNDITRIYKAN
                     VKALLPLKPQTIDMPIGKDKFHSNKRRVSPTGQRAITHILTSKMIKEAVCQLEIKLDT
                     GRTHQIRVHLAEIGHPVIGDPLYGDSTLRQLELESYKIEFVHPLTKEVISVSLDD"
     gene            10603..11715
                     /locus_tag="P6L01_001303"
     CDS             10603..11715
                     /locus_tag="P6L01_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HDP3030378.1"
                     /translation="MEQRTRLALLKEIAEFLNEETEMYSMTQGALKYLIEGSNFTTGW
                     IFFINSVGEHELVSHVALPQSLTADHCHYIKDGSCWCVKAFNQRRLMKASNIVNCSRI
                     NLASKAFPSQNDNITHHATVPLKSGQEQFGILNVASPNTEIYSDEDLELLESVAFQLG
                     SAIKRIYLTDREKEAAKINERNRLARDLHDSVNQMLFSVKLTAHAAYGMSNESIAKQA
                     FKTIEKTSQNAVNEMRALIWQLKPVGLEQGLIHALTAYSKLMHIQLNVNVEGLIDLSN
                     EIEENIYRALQECINNVKKHADTNKMDLTLKQMNDILYIDVIDYGQGFEIDNVQIASS
                     HGINNIKQRVKLLRGKVTFHSQPTKGTQIQFTIPIK"
     gene            11737..12360
                     /locus_tag="P6L01_001304"
     CDS             11737..12360
                     /locus_tag="P6L01_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDP3030379.1"
                     /translation="MNKVILVDDHYIVRQGLRFLLSTIENIEVLQDFADGETFLEYLK
                     EHEHPDIVLLDLVMPGMNGIEITEYIKAHYPEIKVLVLTSYVDDEHVISAINKGADGY
                     EMKDVEPQQLIETIRRVMNGEKMIHPKAQDVFETVSQKPHYTNKLSKREIEVLREMVK
                     GKTNKEIAETLFVSEKTIKTHVSHIFSKLQVSDRTQAAIYAMENKLI"
BASE COUNT         4517 a         1701 c         2273 g         4102 t
ORIGIN      
        1 aaaagtggac tcaaatggca cacgctttaa tagactctat gtcaaattgt aatgatgagt
       61 tcaatattgg aagttaagca actatgcatt gtttaacttc tttttttggt attacattaa
      121 tgataatgat tatattatct ggggttatat atttgtttca acttttctaa taatatattt
      181 gtaaaagtgc tattttattg tgctttatac caagtcgatt caatttatct atcttaatag
      241 taatatggct attttaatat ttacaattta ttacattatt agtcacagga tgtaattagt
      301 agtattttaa gattagcatg acattgatta gtgagtagat tgtttaaatt tactttattc
      361 attttgtgga ttgttatatt tagattaaat tagccaacta atcattgtta aagttaattt
      421 tctataatat gatgagttta atgttcaaat tggaggtcgt ttatgagtaa cgcaaataat
      481 gctgataata aacagttttt cagtaagatt ttaaattcta ttggtgctgg tgttgttatt
      541 gcacttgttc cgaatgcttt attaggtgaa gtattaaaaa tatttaaaag tggtaatgaa
      601 attttagaat taacgtatca gttagtgata ctaattcaat catttatggc atttattatt
      661 ggggttttag ctgctcatca atttaaattt aatggcgcag gtgctgctat agtaggtaca
      721 tcagctatga ttggttcagg tgctgttgta tatagcaata atagttttat gttaaaaggt
      781 ataggtgaca ttataaatac gagtttggtt gttattattg catgtcttat atatatggta
      841 ttacaaaaca aacttggatc ttttgaatta attatattac cagttttagt acctatcgtt
      901 agtgggggta tcggtctaat cacattacct tacattcgaa aaattactca agcaattgga
      961 aatgtaattc attcttttac agacttaaat ccgttgttaa tgtctatact tattagtgtg
     1021 gcattttcat tattaatggt aacaccaata tcgttagtag cgattgcaac tgcgattagt
     1081 ttaaacggac taggaagtgg agcagcaaac ctaggtatag tggctgcctg tgttactttc
     1141 ttatttggct cattgcgtgt caattctatt ggtgtaaatg cagttttact cataggagct
     1201 gcaaaaatga tgatcccagt ttacttgaaa aatttaatta tttcgatacc attgactata
     1261 aatggaataa ttactggaat cattgcttac gttcttcagg ttaaaggaac accattgtct
     1321 gctggatttg gatatacagg attagttggc ccaataaatg catttaaccg aatgtcaggt
     1381 gatccaacaa tgaatattat tttattagca cttggttatt ttgtcatacc atttgtatcg
     1441 gcatttattg ttcatgaatt gtgtaaaaaa ttcattccta tatattctaa tgatatttat
     1501 aaatttgaag ttccaaaaca ataattgata gcttgatgac aagaataagt tttaatatat
     1561 gataaatata ggcgttactg tcaataagat attgatagta gcgctttttt gattgatttt
     1621 aaaatgtgag atttgttcac gtataatagc ggatttttta tgtacagtct tttaaaaaga
     1681 agcttttctt tatattgata aaaatacatt tataaaacac tattgcatca aaatgtatta
     1741 atatgtataa ttatgaatat taattaggga gggctgtata aatgaagtgt ttaattaggt
     1801 ttatattagt attagggttg ttaattagca gtgctatggt gtatatcaat cccacagcac
     1861 atgctgaaca agatcaaaca tgggagaaga ttaaagaacg cggtgaactt agagtgggtc
     1921 tttctgcaga ttatgcacca atggaatttg agcatacagt taatggtaag actgagtatg
     1981 caggtgtaga tattgattta gctaaaaaaa ttgcgaaaga taataattta aaattaaaaa
     2041 tcgtcaatat gtcatttgat agtttgttag gagctcttaa aactggaaaa attgatatta
     2101 ttatttccgg aatgacttca acgcctgaac gtaagaagca agttgatttt tcagattcat
     2161 atatgatgac taaaaatatc atgcttgtaa agaaagataa agttaatgaa tataaagata
     2221 tcaaagactt taataataaa aaagtagggg cacaaaagag aactgaacaa gaaaaaatcg
     2281 ctcaaaccga aattgaaaat gcatctatta cttcattaag ccgtttacca gacgttatat
     2341 tagcattgaa aagtggaaag gttgaaggtg ctgtagttga aaaacctgta gcagaagcat
     2401 atttaaaaca aaatcctaaa ttaggaattt ccaatgtgaa atttaatgaa gaagaaaaag
     2461 atacagtgat agctgtgcca aaagattcac caaaattatt gtcacaaatt aataaaacga
     2521 ttaaagaggt taaagataaa ggattaatcg ataaatatat gactaatgct gcaaatgcga
     2581 tgaatgatga cagtggtttt atttctaagt atggtagttt tttcttgaaa ggaattaaga
     2641 ttacaatatt aatttcactt atcggtgttg cattaggttc tattttaggt gcattcgttg
     2701 cgttaatgaa attaagtaaa ataaaaatta tttcatggat tgcttctatc tatatcgaaa
     2761 tattaagagg aacaccaatg ttagttcaag tatttatcgt attctttggt ataactgctg
     2821 cattaggact agacatttca gctcttgttt gtggaacaat tgcattagtt attaattcct
     2881 cagcttacat tgctgaaatt attcgtgcag gtataaatgc tgttgataaa ggacaaatgg
     2941 aagccgcacg tagtctaggt ttgaattata gacaaacgat gaaaagtgta attatgccac
     3001 aagcaattaa aaatatttta ccagctttag gtaatgaatt tgtcacttta attaaagaat
     3061 catctattgt gtcaacaatt ggagttggcg aaattatgtt taatgcacaa gtggttcaag
     3121 gtatttcatt tgacccattc acaccattac tagtggcagc agcattatac tttgtcttaa
     3181 catttgtact tacacgcatt atgaacatga ttgaagggag attgaatgcc agtgattaaa
     3241 ataaacaatc ttaataaagt ttttggagat aatgaagttt taaaagatat caatcttgaa
     3301 atcaatcaag gggaagtagt agcaataata ggtccatctg gtagtggtaa aagtacattg
     3361 ttaagatgta tgaatttatt agaagtaccc actaaaggtc aagtgatttt tgaaggcaat
     3421 gacttaacgg aaaaagggac acaagtagat aaactacgtc aaaaaatggg tatggtattt
     3481 caaaacttca acctatttcc acataaaaaa gttgtcgata atattatttt agctcctaaa
     3541 ttattaaaga aagataataa cgatgaatta cataaggaag cattgtcgtt attagataaa
     3601 gtgggattaa aagaaaaagc agatgtatat ccgaatcaat tatcaggtgg tcaaaagcaa
     3661 agggttgcaa ttgcaagagc tttagcaatg catccagatg ttattttatt cgatgaacca
     3721 acttcagcat tagatcctga ggtagttggt gatgtattaa aagtaatgaa agacctagcc
     3781 aaagaaggta tgaccatggt ggttgtgaca catgaaatgg gatttgccaa agatgtaagt
     3841 gacaaagtca tatttatggc agatggcgtt gtcgtagagt caggcacacc agtcgaaata
     3901 tttgaacaac cgcaacatga aagaacacaa aatttcttag caagagtatt ataacaacct
     3961 aacgaagctt gaatatatga tgcgcaccac aaagttatat catatattca agctttttat
     4021 tattaaaatt atgtgaaatg aaacctcaat atgaataatg gttgtgttaa aataacatag
     4081 tattatttta tggggggcat acgattggat acaaagcagt taaagcaaga tatcattgac
     4141 tatgcatata caattggtat cgacagtatt ggatttacaa ctgccgatcc ctttgatgaa
     4201 ttgaagcaaa agctagaagc atatcatgca aatggttatg cctcaggatt tgaagaatct
     4261 gatattgctt tacgaacgga gcctaaatta tccttaccaa cagcaaggtc aatcatagca
     4321 attgcagttg gttatcctaa caaactgaaa ggtgcaccta agagtgttag aggtgatcgc
     4381 agaggcttat ttgctagagc atcgtggggt caagattatc atacaattat gcgtaaacga
     4441 ttagacatgt tagctgcatt tattgaatct aaagttccag atgttgaaat caaatctatg
     4501 gtagatacgg gtgtattatc agatagggca gtagcagaac gtgcaggttt aggatttgtt
     4561 ggtcgtaatg gctttgtcat caatcctaaa ctaggaacat ggacatacct cggtgaaatg
     4621 ttagtcagta taccttttga acctgatgat ccattattag atagctgtgg tgattgtaca
     4681 atttgtgttg atcgttgtcc aacaagtgca ttagttggaa atggccaatt aaatagtcag
     4741 aaatgcatta gttttttaac gcaaacaaaa ggctatatgc ctgaccaata tcgttataaa
     4801 attgggaata gattatatgg ttgcgatacg tgtcaacaag tttgtccgaa aaatagaggt
     4861 attaataccg aacaagatga catcattttg gaaccagaaa ttttaaagcc aagattagta
     4921 cctttactac gtatgtctaa taaagaattc aaacaaacat atggtcacct tgcaggtgct
     4981 tggcgtggta aaaagccgat acaacgaaat gctattttag cattagcgca ttttaatgaa
     5041 gtggatgcaa ttccagaatt gaaaaaagtt gcaacaacag atgaaagacc gatgattcga
     5101 gcaactgctt attgggccat tggtcaaatt cttggggaag aagcaagaga ttttatcaat
     5161 gctaattatg atcaagagga tgcagaagtt cagaatgaaa tgataaaagg attagacaca
     5221 aggagagaat agtaatatga caaatcatat cgttttatat caaccagaaa tcccagctaa
     5281 tactggtaac attgcacgta cgtgtgcagg tacaaacaca catttacatt taattaaacc
     5341 tcttggattt agaactgatg ataaaatgtt aaaaagagcc ggtttagatt attgggaatt
     5401 tgtgaatatt acgtatcatg atagtattga agcatttttt gaagccacaa atggcgagta
     5461 ttacttatta acaaaatttg gtaaaaagac ttatagtgat tttgattttt caaatcatga
     5521 caaagatttt tacttcattt ttggaaaaga gacgacagga ttaccagact gggtgaaaga
     5581 gaaatatcaa gacacagcgt taagaattcc tatgagtgaa catattcgtt cattaaattt
     5641 atcaaatact gctgcgttgt taatttatga agcgttacgt caacaagatt ttccaggatt
     5701 gaattaaaat tcgtctattt tcattataaa aatgatactt aagtgctata tttagagtat
     5761 aaccatgtta aagttaaatg gtttgatata ttgaaagtta gggtataata attattaata
     5821 tcgccgtttt tatgtaaaat gcaaactaag aggagtgtag aaatcatggc aatgaacttt
     5881 aaagtctttg acaatagtca acttgtagca gaatatgctg ctgatattat tagaaagcaa
     5941 tttaacaata atcctactac aattgcaggt tttcatttag atacagatca agcgccagtt
     6001 ctagatgaat taaagaaaaa tgttgaaaaa catgctgttg attttagcca aataaatatt
     6061 ttagattatg acgataaaaa atcatatttc gaagcgttag gtgtaccagc aggtcaagtt
     6121 tatccaattg cttatgaaaa agatgcaatc gaattaatcg ctgataagat taaaactaaa
     6181 gaaaataaag ggaaattaac attacaagtt gtttctatcg atgagcaagg taagttaaat
     6241 gttagtattc gtcaaggact aatggaagca agagaaattt tcttagtagt gacaggtgct
     6301 aataaacgag atgtagttga aaaattatat caagaaaatg gtaaaacaag cttcgaacca
     6361 gccgatttaa aagcacatag aatggtaaat gttattcttg ataaagaagc ggctgcaggt
     6421 ttacctgaag atgttaaagc ttactttacg tcacgctttg cttaattaaa aatctgtgag
     6481 gtattgatta tgtctaaaga caaagatcca aaattaaatt atcatgaaga agaaaacagt
     6541 atggtaacgg attttgaaga tttaaaagaa ttaggtaaag aaatggaaca aatctctgat
     6601 caaaatgatc aagaaaaaaa ttctgaagaa gacagtcagt aaagtattat aattgtgtaa
     6661 tattcagtta tttatcatgt aaataatgta tagtatagaa catatatcaa ataggattga
     6721 ttatcttgaa tatgaagtta ggtagcattt tgttacctag cttttttatt atattcatag
     6781 aagctgactt tctgccagct tctgagtttg tggcccgttt ccccaacttg cactgtttgt
     6841 agaaattaga aatccgattt cactatgttg gggcctcgcc aacttgcatg aaatagtaag
     6901 ctgactttcc accagcttct atgtttgtgg actgcagtta actactgcca atataacttt
     6961 gtaaagcata aaacattgat ttgtgtacca gacatagtaa tatatcaaaa gttcaatttt
     7021 aaatattaaa acaccattta aacggaattg ataattaatt actccagaca taattgaaag
     7081 aagtcatcta tcagtaatat ttttaatgaa atcattaaac cgcgtttctt aatatgccaa
     7141 gattatgata tgctattgaa gaatgaaatt agaggagatt tatatattta tgttaggaaa
     7201 atactggatt caattattga tagcaacagt tattataagt ttaatatcaa ttaaagcttt
     7261 tccattggca attggggcat tatatttacc tgttattttc aaagtaataa aattacaact
     7321 caatttatca aaaggattaa tcgatgatgt taatgcgcaa acattcatta aaagtaatca
     7381 atcaggtatt gtaataagtg taatttgttg tttattaata acaggaatat tgtattatac
     7441 acttgatggg ttttatgcga gtttaacagg agttttaggt acgcttgttg ctctaaatcc
     7501 atatacgaca attgttagtg ctgtattata tatacttaca gctattgcta cggttgaagc
     7561 tactaaaaca aagtatcgta attaaactta gttcaacaat aactaaataa tgatagataa
     7621 cacactaatc gtatgaccgt atcactgtaa taaagggtga gtacgtgcga ttagtgtatt
     7681 tttatttcaa attatgaaaa cgttttaatc ataaattgct caattaaatt atttaatgat
     7741 atactacagt taaataaaca gtgataaggg gagaaattga atgtcagtaa gaattgaaca
     7801 tgatactttt ggagaaatag aagtacctgc agataaatat tggggtgctc aaacagaaag
     7861 aagtaaacgc aatttcccag ttggtaaaga gcgtatgcca atcgaagtag tttatggttt
     7921 tgcacaacta aagcgtgcag cagcattagc taattttgat ttaggaaaat taagcgaggc
     7981 aaagaaagat gccattgtat acgcatgtga tcaaatttta tcaggtgaat tagatgaaca
     8041 cttcccacta gttgtatggc aaacaggaag cggtacacaa agtaatatga atgtgaacga
     8101 agtagtaagt tatgttgcta atatgtattt aaaagatcat caaattgatg aaagtatcca
     8161 cccaaatgat gatgtaaatg aatcacaaag ttcgaatgat acattcccaa ctgctatgca
     8221 cgttgcatta tatcaagagg ttgaaacaaa attagaacct gcattaaaac ttttaagaaa
     8281 tactttgaaa gaaaaagaag acaaatttga atcaattatt aaaattggtc gtacacattt
     8341 acaagatgca acgccgatca aactaggaca agagattagt ggctggcgtt atatgcttga
     8401 ccgttgcgaa ataatgttat ctgaatctaa gaagcacatt ttaaatcttg ccatcggtgg
     8461 tacggctgtt ggtactggta ttaatgcgca tcctgaattt ggtgataagg tggcacatta
     8521 tatttcagaa aatacgggtt atccatttgt atcttctgaa aataaattcc acgcacttac
     8581 agcgcatgat gaagttgttc aattgcacgg aacattgaag gcattagctg gagatttaat
     8641 gaaaattgct aatgatgtga gatggttggc ttcaggtcca cgagctggtt tggcagagat
     8701 ttccatccct gaaaatgaac caggttcatc aattatgcct ggtaaagtta atcctacaca
     8761 atgtgaaatg ttaacaatgg ttgcagtcca agtaatgggt aatgatacag ttgttggctt
     8821 cgcaagttca caaggtaact ttgaattgaa tgtttataaa ccagttatta tgcataatac
     8881 actacaatca atttaccttt tagctgatgg tatggaaaca tttaataaca attgtgcagt
     8941 gggcattgag ccaatcgaag agaatattga taattattta aatcaatcat taatgttagt
     9001 tactgcatta aatccacata ttggttatga aaaagcagct caaattgcta agaaagccca
     9061 taaagaaggt ttaactttaa aagaatctgc aattcaaact ggatatgtta cagaagaaca
     9121 atttgaagca tggattaaac cagaagatat ggtagatcct cattaattaa atattaatat
     9181 aaaataaagt ttgggcatta tagtgttcaa gctttatttt ttgtctatgt caaattgaac
     9241 ttataagtcc gatttgatat agagccatga aaaggaaaaa cttttcatgg agtccgtttt
     9301 attatagaac ctatttttta tatgatgaat tgttgtacat gtattggcca aacaacctgt
     9361 gataaaaatc ttatgatgtc atgtattgtc catagataca cgtaacgcag ttgaaaaaga
     9421 ctacaagtca ttatccaagg atgttataaa atgaataatt gctaaaatat caaaatataa
     9481 aatcctaagc atcgtaaaaa acaaaaaaac cgaacatctg aaaaaattaa taattttcca
     9541 tctatccgat ttaagttgat acttgattta gttacaatgc ggtattgcat gtttaaatat
     9601 catgcaaaac taatcaaatt agtcatccaa agaaacggaa atgacttcct tagtcaaggg
     9661 atgcacaaac tctattttgt aactttcaag ttctaattgt cttaacgttg aatcaccata
     9721 taaaggatca ccaataacag ggtgaccaat ttcagctaaa tgcacacgaa tttgatgagt
     9781 acgtccagta tccaacttga tttcaagttg gcacacagct tcttttatca tttttgaagt
     9841 taaaatgtgt gtaattgcac gctgtccagt aggagacaca cgtcgtttat tcgaatggaa
     9901 tttatcttta ccaattggca tatcaatcgt ttgtggtttt aaaggtaaaa gtgccttaac
     9961 atttgctttg tatatccgcg taatatcatt gtcttctaac atgcgatcaa gaattttttt
    10021 cattaaagga tttttagcaa caattaataa tcctactgtt tcctggtcca gtcgatgaat
    10081 tggttcgaca tagtcactat caatagtgta aatcacatga ttcattaaag tattgctttc
    10141 ttttaaatca ttagggtgag tcttaacacc tttaggtttc attacgattg ccatatcatc
    10201 gtcttcgtat ttaatttgtg cataacgata acttggaaca taattacttt tttcatcgat
    10261 ggttggaaca aatacatcgt cgccagtatg cactttatcc attaatcgcg caggtttatc
    10321 attaatagta atatctttag acatatttaa atgatgtaaa tcttttttag gtaccttaag
    10381 ttgttggaaa atatctcgta aacttaagtc attaaagttt tctggtattt taaatttcat
    10441 aaaatcctcc tatagtcttt aatatcatat cacaaatcag ttctcctaaa tataactatt
    10501 gagtaatcaa ggccatttta tcatgttcac gtctaagatt taaaaatata ataactcaat
    10561 gtataatagt taatacacgt aaaaatgaat tggagcgatt tgatggaaca aaggacgcga
    10621 ctagctttat taaaagagat tgccgaattt ttaaacgaag aaactgaaat gtatagcatg
    10681 acccaaggtg cgttaaaata tttaattgag ggcagtaatt tcacgacagg ctggatattt
    10741 ttcatcaata gcgtaggtga acatgaactt gtatcacatg tggctctacc acaatctttg
    10801 acagcagacc actgtcacta tattaaagat gggtcttgtt ggtgtgtgaa agcatttaat
    10861 caacgtaggc taatgaaagc gtcgaatatc gtcaattgtt ctcgtattaa cttagcctct
    10921 aaagcatttc ctagccaaaa tgacaatatt acacatcatg caacggtacc acttaaatcg
    10981 ggtcaagaac aattcggcat tttaaatgta gcgtccccaa atactgaaat atatagtgat
    11041 gaagatttag agttgctaga gtctgtcgca tttcaattag ggtcagccat caaacgtatt
    11101 tatttaacag accgtgaaaa agaagcagca aaaattaatg aacgtaatcg tttagctaga
    11161 gatttacatg attcagtaaa tcaaatgtta ttttctgtaa aactaacagc acatgctgcg
    11221 tatggcatgt caaacgaatc catcgctaaa caagcattca agacgattga gaaaacgagt
    11281 caaaatgccg ttaatgaaat gcgtgcattg atttggcaac ttaagccagt tggacttgag
    11341 caaggtttaa ttcatgcttt gacagcatat agtaaattaa tgcatataca attaaatgta
    11401 aatgtagaag gtttaatcga tttatctaat gaaatcgaag aaaacatata tagagcatta
    11461 caagagtgta ttaataatgt taagaaacat gctgatacga ataaaatgga tcttacatta
    11521 aaacaaatga acgatatact atatattgac gtgattgatt atggtcaagg atttgagatc
    11581 gataatgttc agatagcttc atcacacggt attaataaca taaaacaaag agttaaatta
    11641 ttaagaggta aagtgacctt tcattctcag ccaacaaagg gtacacaaat acaattcaca
    11701 attcctataa aatagcttga tttaaggaga taacccatga acaaagtaat attagtagat
    11761 gaccattata ttgtgcgaca aggattgcga tttttattat ccacgattga aaacatagaa
    11821 gttttacaag actttgcaga tggagaaaca tttttagaat atttaaaaga gcatgagcac
    11881 cctgatattg tgctattaga tttagtgatg cctggcatga atggtattga aattacggaa
    11941 tatattaagg cacattatcc ggaaattaaa gttttggtat taacaagtta tgttgatgat
    12001 gaacatgtaa tttcagcaat caataaaggt gctgatggtt atgaaatgaa agacgttgag
    12061 cctcagcaat taattgaaac tattagacga gttatgaacg gtgaaaaaat gatacatcct
    12121 aaggcacaag atgtattcga aacagttagc caaaaaccac actacacgaa taagttgtca
    12181 aagagagaaa ttgaagtgtt acgtgaaatg gttaaaggta aaacaaataa agagattgca
    12241 gaaactttat ttgtatctga aaaaacaatt aaaacacatg tcagtcatat atttagtaaa
    12301 ttacaagtta gcgatcgtac acaagcagca atttatgcaa tggaaaataa gttgatttag
    12361 aatgagcttt taaatatttg tcgaaaaata gacaattttt aatctgattt tgatgaattt
    12421 tttatagcga tagggtttat tatacagttt tctttgtaaa tttgatatca ttataaaagt
    12481 gtaagtttta tcaaatctag tgattgtgca taaaatttta cttcatttac tttcgcttca
    12541 ttataaagta aacttaactc ttgttgactt gatcaaaatt taatacaaat gtg
//
LOCUS       DAOBBH010000064        12481 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.064, whole genome shotgun sequence.
ACCESSION   DAOBBH010000064 DAOBBH010000000
VERSION     DAOBBH010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12481
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.064"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(236..486)
                     /locus_tag="P6L01_001305"
                     /pseudo
     CDS             complement(236..486)
                     /locus_tag="P6L01_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187510.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            872..2305
                     /gene="eap"
                     /locus_tag="P6L01_001306"
                     /gene_synonym="map"
     CDS             872..2305
                     /gene="eap"
                     /locus_tag="P6L01_001306"
                     /gene_synonym="map"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001806245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular adherence protein Eap/Map"
                     /protein_id="HDP3030380.1"
                     /translation="MKFKSLITTTLALGVIASTGANFNTNEASAAAKPLDKSSSTLHH
                     GHSNIQIPYTITVNGTSQNILSSLTFNKNQNISYKDIENKVKSVLYFNRGISDIDLRL
                     SKQAEYTVHFKNGTKRVIDLKSGIYTADLINTSDIKAISVNVDTKKQPKDKAKANVQV
                     PYTITVNGTSQNILSNLTFNKNQNISYKDLEGKVKSVLESNRGITDVDLRLSKQAKYT
                     VNFKNGTKKVIDLKSGIYTANLINSSDIKSININVDTKKHIENKAKRNYQVPYSINLN
                     GTSTNILSNLSFSNKPWTNYKNLTSQIKSVLKHDRGISEQDLKYAKKAYYTVYFKNGG
                     KRILQLNSKNYTANLVHAKDVKRIEITVKTGTKAKADRYVPYTIAVNGTSTPILSKLK
                     ISNKQLISYKYLNDKVKSVLKSERGISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIF
                     TPNLFSAKDIKKIDIDVKQYTKSKKNK"
     gene            2316..2606
                     /locus_tag="P6L01_001307"
     CDS             2316..2606
                     /locus_tag="P6L01_001307"
                     /inference="COORDINATES: protein motif:HMM:NF015598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDP3030381.1"
                     /translation="MKFPVTINKFENIVSNEFVFYNASKITINDLSIKLKSAMANDQG
                     ITKHDIGLAERAVYKVYFKNGSSKYVDLKTEYKDERVFKATDIKKVDIELKF"
     gene            complement(2885..4171)
                     /locus_tag="P6L01_001308"
     CDS             complement(2885..4171)
                     /locus_tag="P6L01_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDP3030382.1"
                     /translation="MNPLAQSLNEQLQQSNATAFTMLSDLGQNMFYPKGILSQSAEAK
                     STTYNATIGMATNKDGKMFASSLDAMFNDLTPDEIFPYAPPQGIEELRDLWQQKMLRD
                     NPELSIDNMSLPIVTNALTHGLSLVGDLFVNQGDTILLPEHNWGNYKLVFNTRNGANL
                     QTYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVDAIKA
                     LADKGTKVIAVVDDAYYGLFYEDVYTQSLFTALSNLNSNAILPVRLDGATKEFFAWGF
                     RVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDKEIEQN
                     IQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEELRKHLIEKYSIG
                     VIALNATDIRIAFSCVEKEDIPHVFDSIAKAIDDLR"
     gene            4371..4469
                     /locus_tag="P6L01_001309"
     CDS             4371..4469
                     /locus_tag="P6L01_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030383.1"
                     /translation="MMWWQEGMMTLITGGLLIIFRLWLELKWKNKK"
     gene            4712..4888
                     /locus_tag="P6L01_001310"
     CDS             4712..4888
                     /locus_tag="P6L01_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030384.1"
                     /translation="MKHLTKIFVVLAIILFIIGYYLQATNHESQGIKLLLAAIMFMIC
                     AFISRSNDRRKNSK"
     gene            5146..5526
                     /locus_tag="P6L01_001311"
     CDS             5146..5526
                     /locus_tag="P6L01_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDP3030385.1"
                     /translation="MKIILKNNSDFPIYEQIKQQVKQNILKGHVAPGEHLPSMRELAK
                     DLQVSLITTKRAYEDLEKDGFVTTIRGKGTFVKEQDSSILKEKQFFTIENLVKELVNE
                     AQAIEMSLEELQDILTFIYEEESS"
     gene            5523..6419
                     /gene="pmtA"
                     /locus_tag="P6L01_001312"
     CDS             5523..6419
                     /gene="pmtA"
                     /locus_tag="P6L01_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtA"
                     /protein_id="HDP3030386.1"
                     /translation="MNAIELSNVNYSSDQFNLKNISFKVPQGFVTGFIGRNGAGKTTI
                     IRLIMDLYQPQTGVIRVLEEDMALNPIELKNSIGFVYSENYFNERWTTKQLEKMIAPF
                     YRKWDHQVFEFYLEKFDLPINKSIKTFSTGMKMKLSLAVAFSHHAELYIFDEPTSGLD
                     PLARNELLEIIQQELIDENKTIFMSTHIISDLEKIADYIIHLSDGEVILNGSKEQLLQ
                     RYQVVSGATEDLDDELASLLIYEEHKRTGFIGLTEHAQVFKEILGHKVNITTPSIENL
                     MVYLEKRKPKYHENIKLMEEGF"
     gene            6420..7100
                     /gene="pmtB"
                     /locus_tag="P6L01_001313"
     CDS             6420..7100
                     /gene="pmtB"
                     /locus_tag="P6L01_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtB"
                     /protein_id="HDP3030387.1"
                     /translation="MKALLIRNFKLRRYTLIIYVLLLTLYPFYIMLDSTKFFYLLQSF
                     ISPTILIIWILDAGHLFRLNRRLGGNDSYYFYMSLPVSKKQLLNANYITCIVLTLIGT
                     LVISLYAYEADVIEPNSIYFSTAYAFVISNFLSIPIAFSQFTELRRVKVPYGIYVFTI
                     IILVPFLFSIAIVLVNYFVLSQSSFPDLYSYILNIGFLIISIVILIVNYFKQLNKINT
                     RKFKGGSR"
     gene            7097..7969
                     /gene="pmtC"
                     /locus_tag="P6L01_001314"
     CDS             7097..7969
                     /gene="pmtC"
                     /locus_tag="P6L01_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtC"
                     /protein_id="HDP3030388.1"
                     /translation="MKLEHITKKYGSNVVLNDIDFDFGDSRIVGLIGKNGVGKTTIMK
                     VMNGNIIKFDGKVDIDNADNIGFLIEHPKLYDNKSGLYNLKLFAQVLGKGFDKAYTDK
                     IIDAFGMRPYIKKKVKKYSMGMKQKLAIAVSLMNKPKFLILDEPTNGMDPDGSIDVLT
                     TIKSLVNELDMRILISSHKLEDIELICDRAVFLRDGHFVQDVNMEVGVASDTTIVTVD
                     HKDFDRTEKYLAEHFQLQNVDKADGHLMINAQKNYQVILKALSELDIYPKYIETRKSS
                     LRDTYFNINQRGDK"
     gene            7969..8709
                     /gene="pmtD"
                     /locus_tag="P6L01_001315"
     CDS             7969..8709
                     /gene="pmtD"
                     /locus_tag="P6L01_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtD"
                     /protein_id="HDP3030389.1"
                     /translation="MRILNLVKYDFYSIFKSPLTYLAILVVSSLIATQSILMANSMDN
                     PKHIIVYGSVFAAAKWLLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYIISKTI
                     SIILISILFALIHYVILIVVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLA
                     SQKTAMIFSLGVFLVLIVPFIKPFITFIPRYGEKVLDAFDYIPFAYLTDKMISSNFDF
                     INWQWVISLGSIVIFFILNILYVAKKDI"
     gene            complement(8775..9338)
                     /locus_tag="P6L01_001316"
     CDS             complement(8775..9338)
                     /locus_tag="P6L01_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDP3030390.1"
                     /translation="MTNLETYFKNSQPLNEYIDGMKTNQDTVLSIYQSFELPEDDSRI
                     KKIKEMNYSKVLVITEDWCGDAMMNLPILKHISEALNLEVRVFHRDDDTKLIDQYLTN
                     GKSRAIPIFVFLNDQFEQETVWGPRANEVQKFVTDVRADKLPSKDHPDYADLEKETHL
                     VISNRYKTDASFWKAVYNSILNKLENK"
     gene            9459..9983
                     /locus_tag="P6L01_001317"
     CDS             9459..9983
                     /locus_tag="P6L01_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030391.1"
                     /translation="MLYLNKYKERVTSVRIQNRWVVFILFLICSFGVLIGLYQYRHTK
                     TVDLSNLEINDIKLNEKFDKKGYEVNKKIKFDRFKFYNSKAHPDLTVKVREKDNIVKG
                     IILVRDEKIHTNFDGGIGSPINNAIENLGFGYKRTKVGNDFSSVKYIDRDNHLKLNLL
                     YQDLEIKRIEFFSK"
     gene            10043..10600
                     /locus_tag="P6L01_001318"
     CDS             10043..10600
                     /locus_tag="P6L01_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDP3030392.1"
                     /translation="MNKITYLNELETALDALPRHLRDQKMYEYERYFYEQELNGFDEG
                     EILKKLKDPQDVAAETKARSVIDYAESKPTFENISRAVAASLSLGILSIFVILIPVSI
                     VGLFVLALFLISLLLLFCPIILLASAISRGIVDSISNVFFAISYSGLGLVFIIVIFKI
                     LEYIYRLILKYLLWYIKTVKGSVRK"
     gene            10597..11439
                     /locus_tag="P6L01_001319"
     CDS             10597..11439
                     /locus_tag="P6L01_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDP3030393.1"
                     /translation="MKKFFFIGLLVFVVFFTAATIIWFSYDKNKYGTKQYDKTFKDDA
                     FDNVSINLDSTELRIKRGNQFRVKYDGDNDILINIVDKTLKISDKRSKTRGYAIDMNP
                     FHENKKTLTIEMPDKMIKRLNLSSGAGSVRISDVDLENTSIQSINGEVVIKNSNLDAL
                     DSKTNNSSTYISKSNIKNSNIKVVIGTLQIDKSQIKQSIFLNDHGDIEFKNMPSKVDA
                     KASTKQGDIRFKYDSKPEDTILKLNPGTGDSVVKNKTFTNGKVGKSDNVLEFYTIDGN
                     IKVE"
     gene            complement(11496..>12481)
                     /locus_tag="P6L01_001320"
     CDS             complement(11496..>12481)
                     /locus_tag="P6L01_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDP3030394.1"
                     /translation="VKTGKWLQQTPLLKNREKEWKKRVPRFDIDVNETYQIFQTYAPQ
                     IWEELMGLQSILKMPTRQIILNFGHYRFTDLKESGCTVFQGKDFMVRNYDYHPATYDG
                     RYLLYQPTDSGLAQIGPVSRVTGRMDGMNESGLTMGYNFMHRKKPANGFVCYMIGRLI
                     LENCRNVTEAIQLLKEIPHRSSFSYILMDKSLNHAIVEVTPRSIDVRYDNICTNHFEI
                     LTHENRNYTKESKERLARTISQTNDNLDMTTAFKLFNNPQYEIYSKLFKSWSGTIHTS
                     MYQPETLTAYFTLGENAPPEIIDFKSWLDGQDLNITHFTGNIDTDLTFANK"
BASE COUNT         4474 a         1627 c         2025 g         4355 t
ORIGIN      
        1 tgtcaagcaa tattattata aaattggaat tctgagttgt ctactctaat ttattatatt
       61 tacctataaa aatacacctc aaaaaataga tttttcagtc tagcttttgg ggtgtacatt
      121 ccacacaaac atgtgattat tttgatgttt ctattaaact tgtaatttta aatttaaagt
      181 ccctaaaaag tccctaaaat tttattttat atgaggtatt attgataatg ataaagttat
      241 aaaccttgat attatgctgt tttacttttt gaatgataag taattttatg ttaaaagtct
      301 ccagtttgga tacaaaacgg tcgataacat ataaacgtta tgactaacta acttcaaatc
      361 agtatcatct ttcttagatt cggctttggc actattgtca gtaagtgcac caactaataa
      421 taaatttgct aatgcaagtg ttgcaacttt ttttagtgaa ttggattttg ttttttttac
      481 catcattatc actcctttta tatagcttac aacaaaatag atgcaaaatt gattaactaa
      541 atttaaatta attattaaat cgaatttata tttttaagta aatgtttttt gagtataaag
      601 atgctgtaaa tataatcgac aaatataatc ggaattaaga aatgaagatt gttatgacat
      661 ttaagtttga aacttggtat aaacaaaatc aaacaacatt ttaggtattg taaattagtt
      721 agttaatcaa aattaaaaga acttttaaca gtgttaaaaa attattgtta aagataattt
      781 attattaata ttcagttaat tcaaaaaata gagaaagtct ggctataata aagttgcaat
      841 cacgaattac ataaaaaagg agagataatt tatgaaattt aagtcattga ttacaacaac
      901 attagcatta ggcgttatag catcaacagg agcaaacttt aatactaacg aagcatctgc
      961 cgcagctaag ccattagata aatcatcaag tacattacac catggacatt ctaacatcca
     1021 gattccatat acaattactg tgaacggtac aagccaaaac attttatcaa gcttaacatt
     1081 taataagaat caaaatatta gttataaaga tatagagaat aaagttaaat cagttttata
     1141 ctttaataga ggtattagtg atatcgattt aagactttca aagcaagcgg aatatacggt
     1201 tcattttaaa aatggaacaa aaagagttat cgatttgaaa tcaggtatct acacagctga
     1261 cttaatcaat acaagtgaca ttaaagctat cagtgttaac gtagatacta aaaagcaacc
     1321 taaagataaa gctaaagcaa atgttcaagt gccatataca atcacagtga acggcacaag
     1381 ccaaaacatt ttatcaaacc taacatttaa taaaaatcaa aatattagtt acaaagattt
     1441 agagggtaaa gttaaatcag ttttagaatc aaatagaggt attactgatg ttgatttaag
     1501 actttcgaag caagcgaaat atacagttaa ttttaaaaat ggaacgaaga aagttatcga
     1561 tttgaaatca ggtatttaca cagcgaattt aatcaattca agtgatatta aaagtatcaa
     1621 tattaacgta gatacaaaaa aacatatcga aaataaagct aaaagaaact atcaagttcc
     1681 atattcaatt aatctaaatg gtacatctac aaacatttta tcgaatcttt cattttcaaa
     1741 taaaccttgg acaaattaca aaaatttaac tagtcaaata aaatcagtac tgaagcatga
     1801 tagaggtatt agtgaacaag atttaaaata tgctaagaaa gcttattata ctgtttattt
     1861 taaaaatggt ggtaaaagaa tcttacagtt aaattcaaaa aattacacag caaacttagt
     1921 tcatgcgaaa gatgttaaga gaattgaaat tactgttaaa acaggaacta aagcgaaagc
     1981 agacagatat gtaccataca caattgcagt aaatggcaca tcaacaccaa ttttatcaaa
     2041 actaaaaatt tcgaataaac aattaattag ttacaaatat ttaaacgaca aagtgaaatc
     2101 tgtattaaaa agtgaaagag gtatcagtga tcttgactta aaatttgcga aacaagcaaa
     2161 atatacagta tatttcaaaa atggaaagaa acaagtagtg aatttaaaat cagacatctt
     2221 tacacctaat ttatttagtg ccaaagatat taaaaagatt gatattgatg taaaacaata
     2281 cactaaatca aaaaaaaata aataaatcta ataatgtgaa attcccagta acaataaata
     2341 aatttgaaaa catagtttca aatgaatttg tgttctataa tgcaagcaaa attacaatta
     2401 atgatttaag tataaaactt aaatcagcaa tggcaaatga tcaagggata actaaacatg
     2461 acataggact tgctgaacgc gcagtgtata aagtgtattt taaaaatggt tcgtcaaaat
     2521 atgtagactt aaaaactgag tataaagatg aaagagtatt taaagcaact gacattaaaa
     2581 aggtagatat tgaacttaaa ttctaatctt taattttata ataaggcatc tcacaatcgt
     2641 ggggtgcctt ttacatttgt agagatgtga tgcttgaatg tttgaagtga tttgccaaaa
     2701 gttatataaa tttgtaccaa ataaattaaa ttttgttagg agagatgtat aaaactaatt
     2761 aggtatagtg tttgacaagt tgctgaataa aatatatctt atgatacttt gaaagaagga
     2821 ataattttaa aaataaaaaa ccaataatcc gagtcatact catcagatta ttggttgaaa
     2881 ttaattatct taagtcatca atcgctttag caattgaatc aaatacatgt gggatatctt
     2941 ctttttctac acagctaaac gcaatacgaa tatctgttgc attaagtgca ataacaccaa
     3001 ttgaatattt ttcaattaaa tgtttacgaa gttcttcagg gtcaacatca tgaactttta
     3061 ttgccataaa gtatccagag ttaaagtcat aggcttgcca atgtgaatga tattgatcag
     3121 catagacaac ttccttagtg acttcatagc gttcttttaa tgtttgaata ttttgttcga
     3181 tttctttatc gaattgttta ttatttttta atacatgctt aacagcgctt tgtgttggaa
     3241 gaggtccact agaaatgtta cttcgtataa gaccttttac tttggcttct aatacttctt
     3301 tagttgtttg atcagacgtt ccaaatgtca taaacccaac acggaatccc catgcgaaaa
     3361 attcttttgt tgcaccatct aaacgaacag gtaatattgc atttgaattt aaattagata
     3421 atgcagtaaa taatgattga gtatacacat cttcatagaa taaaccatag tatgcgtcat
     3481 ccacaacagc tataactttt gtacctttat cagctaatgc tttaattgca tcgacgatgg
     3541 tagtcacttc tttatgcgta ggtgtgtaac ctgtcggatt attaggataa tttaaaatca
     3601 taatgacttt atctttattg tatgattgta aagcttctac aagtgaatca gtagtataat
     3661 gcccgtcttt atcaaagata ggatatgttt gaaggtttgc accatttctc gtattgaaaa
     3721 caagtttgta attaccccaa ttatgctctg gtaacaagat agtgtcacct tgatttacaa
     3781 ataaatcgcc aactaaagat aaaccatgtg ttaatgcatt cgtaacaatt ggtagtgaca
     3841 tgttgtcgat tgatagctct ggattgtcac gcaacatttt ttgttgccat aaatcacgta
     3901 attcttcgat gccttgtggt ggcgcataag ggaatatttc atctggagtt aaatcattaa
     3961 acattgcatc taaagatgat gcaaacattt ttccgtcttt gtttgtcgcc atacctatag
     4021 ttgcattata tgttgtactc tttgcttcag cagattgaga taaaatgcct tttggataaa
     4081 acatattttg acctaagtca gataacattg taaaggcagt tgcatttgac tgttgaagtt
     4141 gttcatttaa gctttgggct aaaggattca ttattgctta acctctcttt aaaaaaattg
     4201 tatgcctata tattatcttt aattatacca aagataaagt gttgttggtg aattaacgaa
     4261 taatatgtaa aatctgctgc caaaatacga acatatgttc tgtttttatg ttagaatagg
     4321 tgtaggtcat ccttcagtat gatactttag tcgaacggag gtgtagtgta atgatgtggt
     4381 ggcaagaagg gatgatgaca cttattacag gtggcttact aattattttt cgactttggt
     4441 tagaactgaa atggaaaaat aaaaaatgat cctacacaca taaataaccc caagtctgct
     4501 ggtaccagac ttggggttgt gtagtataat tctgtggata cacttaaata atatcattga
     4561 acacaatata tcgcaatcaa taatcatttt gattgaatgg ttttgttgcg ctattttagt
     4621 tgaaagtgat tagtaccttg aacttttgaa attcaataac ttattaaaaa aatttatatt
     4681 tgttataatg tcgatgttga ggtgaaataa attgaaacat ctaacaaaaa tatttgtagt
     4741 acttgcaatt attttattta ttattggtta ttatttacaa gcaactaatc atgaaagcca
     4801 aggtataaaa ttattattag cagcgattat gtttatgatt tgtgctttta taagtagaag
     4861 taatgatcga cgtaaaaata gtaaatagaa ttgactgttt aaaacgattg tttatgttga
     4921 cgctacggtt gtcacgtaga gattaaagac attcgtttta gcagtctttt ttattgaagt
     4981 agacaatgca agattaaatg gtagtgtcat ttcattttat tcaactgcta gtaatacaat
     5041 actcagtgtt atttctgaag actggttgat aatggacatg catatttaca aaactgtgta
     5101 tattgtgtat attgtatata tacagaagtt gataggggga cgttgatgaa aataatttta
     5161 aaaaacaata gtgattttcc gatttatgaa cagattaagc aacaagtaaa acaaaatatt
     5221 ttaaagggac atgttgctcc tggagagcat ttgccgtcaa tgagagaact tgccaaagat
     5281 cttcaagtaa gtttgattac taccaaacgt gcttatgaag atttagagaa agacggtttt
     5341 gttacaacaa ttagaggaaa agggaccttt gttaaggagc aagatagttc tattttaaaa
     5401 gagaaacaat tttttaccat tgaaaatttg gttaaagaat tggttaatga agcgcaagcc
     5461 atcgaaatgt cacttgagga acttcaagat attttaacgt tcatttatga ggaggaatca
     5521 tcatgaatgc catagaatta agtaatgtta attattcaag tgatcagttt aatttaaaga
     5581 atatctcttt caaagttcct caaggattcg tcactggttt tattggtaga aatggcgctg
     5641 gtaaaaccac aataataagg ttaattatgg atttgtatca accacaaact ggtgttatta
     5701 gagtattaga agaggatatg gctctcaatc cgatagagtt aaagaatagt attgggtttg
     5761 tttactctga aaattatttt aatgagagat ggactacgaa gcaacttgaa aaaatgattg
     5821 ccccttttta tcgtaaatgg gaccatcaag tatttgaatt ttatcttgaa aagttcgatt
     5881 taccaatcaa taaatcaata aaaacatttt cgactgggat gaaaatgaaa ttatcactag
     5941 cagttgcatt ttcacatcat gctgaattgt atatttttga tgagcctact tcaggtcttg
     6001 atcccttagc tagaaatgag ctattggaga taattcagca agaattaatt gatgaaaata
     6061 aaacaatatt catgtcgaca cacattatct cagatttaga aaaaatagct gattatatca
     6121 ttcacttaag tgacggtgaa gttatcttaa atggttcaaa agaacaactg ttacaaagat
     6181 atcaagttgt gagtggtgca actgaggacc ttgatgatga attagcatct ctacttattt
     6241 atgaagaaca taagcgtacg ggatttatag gattaacaga acatgcgcaa gtatttaaag
     6301 aaattttagg tcataaagtg aatatcacta caccttctat tgagaactta atggtttatc
     6361 ttgaaaagag aaaacctaaa tatcatgaaa acatcaaatt gatggaagaa ggtttttaaa
     6421 tgaaggcatt gttaattagg aattttaaat tacgacgata tacacttatc atctatgttc
     6481 tattattaac cttatatcct ttttatataa tgcttgactc aacaaagttc ttttacctat
     6541 tgcaatcttt tataagtcca acaatattga ttatttggat tttagatgct ggtcacttat
     6601 ttagactcaa tcgtagatta ggtggtaatg attcatatta tttttatatg agtttaccag
     6661 tgtcaaaaaa acaattgctt aatgccaact atattacatg cattgtttta acattaattg
     6721 gaacgcttgt tattagttta tatgcttatg aagcagatgt gattgaacca aattcaatct
     6781 atttttcaac tgcatatgca tttgtcatat ctaacttctt gtctatacca attgcattta
     6841 gtcaatttac agaattgcgt agagtcaaag tgccatatgg tatatacgtg tttactatta
     6901 tcattttagt tccattttta ttttcaattg caatagtgtt ggtgaattat tttgttctaa
     6961 gtcaatcatc attcccagat ttatattcat atattttaaa tattggtttt ctaattatca
     7021 gcattgttat acttattgtt aattatttta aacagctcaa taaaataaat actagaaaat
     7081 ttaaaggagg cagtcgatga aattagaaca tattacaaaa aaatacggct caaatgtcgt
     7141 tttaaatgat attgattttg actttggcga tagtagaatt gtcggattaa taggaaaaaa
     7201 cggtgttggt aaaacaacca ttatgaaagt aatgaatggt aatattatta aatttgatgg
     7261 aaaagtagat attgataatg cagataatat cggtttttta attgagcatc ctaaattata
     7321 tgataataaa tcaggattgt ataacttgaa attatttgca caagtattag gtaagggttt
     7381 tgataaagca tacacagaca aaattataga tgcatttggt atgagacctt atattaaaaa
     7441 gaaagttaag aaatattcaa tggggatgaa gcaaaagtta gcaattgcag tatctttaat
     7501 gaataaacct aaatttttaa tcttggatga gcctacaaat ggtatggatc cagatggctc
     7561 aattgatgtg ctgactacaa ttaagtcttt agtaaatgaa cttgatatga gaattctaat
     7621 atcaagtcat aagttagaag atattgaatt aatttgtgat agagctgtat ttttaagaga
     7681 cggacatttt gttcaagatg taaacatgga ggtaggtgtt gcatctgaca caacgatagt
     7741 tactgttgat cataaagact ttgatagaac tgaaaaatat cttgcagagc atttccaatt
     7801 acaaaatgtc gacaaagcag acggacattt aatgatcaat gcacaaaaaa attatcaagt
     7861 tatactaaaa gcattatctg aattagatat ttatccgaaa tatattgaaa cacgtaaaag
     7921 ttcattgcgt gatacgtact tcaatataaa tcaaagaggt gataaataat gagaatttta
     7981 aatttagtta agtatgattt ttatagtata tttaaaagtc ctttaacata tttagcgata
     8041 ctagtcgtat ctagtttgat tgcaactcaa agtatactta tggcaaattc gatggataac
     8101 ccgaaacata ttattgtcta tggatctgta tttgctgcag caaaatggtt attgttaata
     8161 attggattaa tgtttgttgt taagacaatt acgcgtgatt tttcacaagg tacaattcaa
     8221 ctatatatga gtaaagttaa aacacgcgtt ggatacatta tttcgaaaac aatttcaatt
     8281 attttaattt caatattatt tgcattaatt cattatgtga ttttgattgt tgtgcaggca
     8341 tctagtaatg gaaaaaattt ggcgttttct aaatatgtag ataatttatg gttcttccta
     8401 atctttttac tattctttgg cttgttttta ttcttaatca cacttgcatc acaaaaaaca
     8461 gcaatgatat tttcattagg tgtattttta gtactcattg taccgtttat taaacctttt
     8521 attacattta tcccaagata cggtgaaaaa gttttagatg cttttgatta tatccctttt
     8581 gcttacttaa ctgataaaat gattagctct aactttgatt ttatcaattg gcaatgggta
     8641 atttcattag gttctatagt gatattcttc attttgaata tcttatatgt cgctaaaaaa
     8701 gacatttaat aaaaataatt ttgaggttgg gaattttaaa ttttcccaac ctcaaagttt
     8761 gtcttattgt aaatttattt attttctaat ttatttagga tggaattata aactgctttc
     8821 caaaatgaag cgtcagtttt atagcggttt gatataacta agtgtgtttc tttttctaaa
     8881 tctgcatagt ctggatgatc tttgctcggt aatttatcag cacgaacatc agttacaaat
     8941 ttttggactt catttgctct tggtccccaa actgtttctt gttcgaattg atcattcaag
     9001 aatacgaaga taggaattgc acgtgattta ccatttgtta aatattgatc gatcagtttt
     9061 gtatcatcat ctctatggaa cacgcgtact tctaaattta atgcttcact gatgtgtttt
     9121 agaattggga gattcatcat tgcatctcca caccagtctt cagtaattac taatacttta
     9181 gaataattca tctcttttat ttttttgatg cgtgaatcat cttctggtaa ctcaaatgat
     9241 tgatagatac tgagaacggt atcttgattt gtcttcattc catcaatgta ttcatttaag
     9301 ggttggctat ttttgaaata agtttctaaa tttgtcattg taaaaacctc ctttagcatt
     9361 tacaacatta taccaattta tagtaataaa aggtaatgaa ataaattaat tgcaaattct
     9421 ttgttaattt ttgttaagga tgaaacggga ggcaccttat gctatattta aataagtaca
     9481 aagaaagggt gacatcagtg cgtattcaaa atcgctgggt tgtgtttata ttatttttaa
     9541 tctgttcttt tggtgtatta attggtttat atcaatatcg tcatacgaaa actgtggatt
     9601 tgtctaatct tgaaataaac gatattaaat taaatgagaa gtttgataaa aaaggctatg
     9661 aagtaaataa aaaaattaag tttgatcgtt ttaaatttta taacagtaaa gctcaccctg
     9721 atcttaccgt taaagtgaga gaaaaggata acatcgttaa ggggataata ttagtaagag
     9781 atgaaaagat acatactaat tttgatgggg gaattggttc gccgataaat aacgcgattg
     9841 aaaatcttgg attcggatat aaaagaacaa aagttggcaa tgatttctca tcggtaaagt
     9901 atattgatag agataaccat ttaaaattaa acttacttta tcaagattta gaaattaaac
     9961 gtattgaatt ttttagtaaa tagctttagg tcttaaagtt ataaaaaacg aataaataat
    10021 tttattggga tgagtgacct atatgaacaa gattacttat ttaaatgaat tagaaacagc
    10081 acttgatgcg ctacctaggc atttaagaga tcaaaaaatg tatgaatatg aacgttattt
    10141 ttatgagcaa gaacttaatg gctttgatga aggggaaatt ttaaagaagt taaaagaccc
    10201 acaagatgtt gcagctgaaa caaaagctag aagtgttatt gattatgctg aatctaaacc
    10261 aacatttgaa aatatttcaa gagctgttgc tgcttcatta agtttaggca ttctatctat
    10321 ttttgtcatc cttataccag tatctatagt tggattattt gtattagcat tatttttaat
    10381 atcacttttg ctgctgtttt gtccaattat tttattagca tcagcaatat ccagaggaat
    10441 tgtagactca attagtaatg tattttttgc catatcatat tcaggattag gattagtatt
    10501 tatcattgtc atatttaaga ttttagaata catttatcgt ttaatcttaa aatatttact
    10561 ttggtatatt aaaactgtca aaggaagcgt tagaaaatga agaaattctt ttttattggg
    10621 cttttagtgt ttgttgtctt ttttacagca gcaaccatta tttggttcag ctatgataaa
    10681 aacaaatatg gtactaaaca atatgataaa acattcaaag acgatgcttt tgacaatgta
    10741 tctataaatt tggatagtac agaacttcgt ataaaacggg ggaatcaatt tagagttaaa
    10801 tatgatggtg acaatgatat attaattaat atagtagata agacgttgaa gattagtgat
    10861 aaaaggtcta agacaagagg atatgcaatt gatatgaatc cttttcatga gaataagaaa
    10921 acgttaacga ttgaaatgcc tgataaaatg attaaacgtt taaatctatc atctggagca
    10981 ggaagtgtta gaatcagtga tgttgattta gagaacacaa gtattcaaag cattaacggt
    11041 gaagtagtta ttaaaaactc aaatcttgac gcactagatt caaagacaaa taatagttct
    11101 acttatatta gtaaaagtaa cattaaaaat agcaatatta aagttgttat tggtacgcta
    11161 caaatcgaca agagtcaaat taaacaatcc atatttttaa acgatcatgg tgacattgaa
    11221 tttaaaaaca tgccatcaaa agtagatgca aaagcttcta ctaaacaagg agatattcgt
    11281 tttaagtatg atagtaaacc tgaagacact atactaaagc taaatccggg aacgggtgat
    11341 agcgtagtta aaaataaaac atttactaat ggtaaagttg ggaaaagcga caatgtttta
    11401 gaattttata cgattgatgg taatatcaaa gttgaataaa taaaggatgt aagcaccgat
    11461 attaggaagc ataatttctc taatatcggt gttatttatt tgttggcaaa agttaagtcg
    11521 gtatctatat tgccagtaaa gtgagtgata ttaaggtctt gaccatctaa ccatgatttg
    11581 aaatctatta tttctggtgg cgcattttct cccaatgtaa aatatgcagt taatgtttca
    11641 ggttgataca ttgatgtatg gatggtgcca gaccagcttt tgaatagttt actgtaaatt
    11701 tcatactgag gattattgaa taacttaaat gctgtagtca tatctaaatt atcattagtt
    11761 tgtgaaatgg tacgcgccag tctttcttta gattcttttg tataattacg attttcatgt
    11821 gttaatattt caaaatgatt tgtacatata ttatcataac gaacatctat tgatctcggt
    11881 gtcacttcaa caattgcatg gttcaatgat ttgtccatca gtatgtagct aaatgagctt
    11941 ctgtgtggta tttctttcaa taattgtatt gcttctgtta catttcggca attttcaaga
    12001 attagacgac caatcatata acatacaaaa ccatttgctg gtttcttccg gtgcataaag
    12061 ttatagccca tagttaatcc tgactcattc ataccatcca ttcttccagt tacccttgat
    12121 acaggaccaa tttgagctaa accgctatct gtaggttgat atagtaagta gcgaccatca
    12181 taagttgcag ggtggtaatc ataatttcta accatgaagt ctttgccttg aaagaccgtg
    12241 caaccacttt cttttaaatc ggtaaaacga taatgtccaa agtttaaaat aatttggcgt
    12301 gttggcattt tgagtatact ttgtagtccc attaattctt cccatatttg aggtgcgtaa
    12361 gtttggaata tttgataagt ttcatttaca tctatatcga aacgtgggac acgttttttc
    12421 cattcttttt ctcgattttt tagaagaggt gtttgttgaa gccatttacc agttttaaca
    12481 c
//
LOCUS       DAOBBH010000065        12399 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.065, whole genome shotgun sequence.
ACCESSION   DAOBBH010000065 DAOBBH010000000
VERSION     DAOBBH010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12399
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.065"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(110..943)
                     /locus_tag="P6L01_001321"
     CDS             complement(110..943)
                     /locus_tag="P6L01_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDP3030395.1"
                     /translation="MEVKTFYNGNTMPQIGLGTFRVENDENCMESVKYAIEQGYRSID
                     TAKVYGNEEQVGAGIRAGLESTGIAREDLFITSKLYFEDFGRENVAAAYEASLSRLGL
                     KYLDLYLVHWPGTNEAVMVDTWKGMEDLYKNNKAKNIGVSNFEPEHLEALLAQVSIKP
                     VINQVEYHPYLTQHKLKLYLAAQHIVMESWSPLMNAQILNDETIKDIAQELGKSPAQV
                     VLRWNVQHGVVIIPKSVTPNRISENFQIFDFELSDEQMTRIDGLNQDKRIGPDPKTFE
                     G"
     gene            complement(1155..2063)
                     /locus_tag="P6L01_001322"
     CDS             complement(1155..2063)
                     /locus_tag="P6L01_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HDP3030396.1"
                     /translation="MDLSSPVVIGLIIAVVIALIFFVLFLVALGSKKKVKRQTEEKYE
                     QQEQNIKKSHEEALEKERIQNKKTITKQQEDYNHMVSTKDREIDALKLFSKNHSEYVT
                     DMRLIGIRERLVKEKRIRPEDMHIMANIFLPKDGFNNIERISHLVLTRTGLYIIDSQL
                     LKGHVYNGISGGQFKDLPPMEQVFDTLDLDKSRPQTIVMDQNDDKRSLSFVNYSDQIE
                     AIKQLAEDLQKELGAKYTPTSILYFNPKNEGDVTISNYNQNSAVKVLVGAEQLDEFFN
                     KFVFHGRIQYNVEDLQQMMDKIESFN"
     gene            complement(2185..3381)
                     /locus_tag="P6L01_001323"
     CDS             complement(2185..3381)
                     /locus_tag="P6L01_001323"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDP3030397.1"
                     /translation="MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVAC
                     ETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQS
                     PDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
                     LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHADDVTLEQIQEDIKA
                     HVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCF
                     SGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKV
                     SEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEEL
                     KDAVKY"
     regulatory      complement(3487..3598)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            3753..5345
                     /gene="pckA"
                     /locus_tag="P6L01_001324"
     CDS             3753..5345
                     /gene="pckA"
                     /locus_tag="P6L01_001324"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDP3030398.1"
                     /translation="MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTE
                     LGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDK
                     KDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
                     APHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMS
                     MHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAK
                     AINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVV
                     PSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSF
                     STCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMV
                     NQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRF
                     EKNFEKFGEKVEHIAEKGSFNK"
     gene            complement(5723..6469)
                     /locus_tag="P6L01_001325"
     CDS             complement(5723..6469)
                     /locus_tag="P6L01_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDP3030399.1"
                     /translation="MPIESFTHQFEEITYLSDDLQVKALMMTPHHEVNRIVVYLRGGK
                     GQVGRVRAGRLMQFSDSQTLVIGPYYRGNNGSEGKDEFYRGDLNDVTQLLRLLHDKYP
                     HAFIHMVGFSRGGLQGLLTFQDLPVTSYTIWGGVSDIDLMYEERVDLRGMLRRMIGHP
                     KKDRAAYEARQAIPNINENSPPILIVHGGKDQQVGIHHAYYLADQLELKGATHETFYQ
                     MAEGHVPRPPAMVETLTYIKEFMKQVELHS"
     gene            complement(6474..6947)
                     /gene="ytkD"
                     /locus_tag="P6L01_001326"
     CDS             complement(6474..6947)
                     /gene="ytkD"
                     /locus_tag="P6L01_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase YtkD"
                     /protein_id="HDP3030400.1"
                     /translation="MKFRDKDNRQVNLTFKKDNEIADGNHVLAIPTFKNQLLFTKHNL
                     RGIEFPGGKRERGESSAEAVTRELYEETGAKVKNIHYIAQYTIETHDQTDFVKDVYFI
                     EVESLVSKNDYLETAGPVLFNCINDIELAQRSFLLQDSTILKCVERVQSLGFYQT"
     gene            7013..7270
                     /gene="yidD"
                     /locus_tag="P6L01_001327"
     CDS             7013..7270
                     /gene="yidD"
                     /locus_tag="P6L01_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDP3030401.1"
                     /translation="MKKIFLAMIHFYQRFISPLTPPTCRFYPTCSEYTREAIQYHGAF
                     KGLYLGIRRILKCHPLHKGGFDPVPLKKDKSASKHSHKHNH"
     gene            complement(7267..8268)
                     /gene="menC"
                     /locus_tag="P6L01_001328"
     CDS             complement(7267..8268)
                     /gene="menC"
                     /locus_tag="P6L01_001328"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDP3030402.1"
                     /translation="MKLTALHFYKYSEPFKSQIVTPKVTLTHRDCLFIELIDDKGNAY
                     FGECNAFQTDWYDHETIASVKHVIEQWFEDNRNKSFETYEAALKLVDSLENTPAARAT
                     IVMALYQMFHVLPSFSVAYGATASGLSNKQLESLKATKPTRIKLKWTPQIMHQIRVLR
                     ELDFHFQLVIDANESLDRQDFTQLQLLAREQVLYIEEPFKDISMLDEVVDGTIPPIAL
                     DEKATSLLDIINLIELYNVKVVVLKPFRLGGIDKVQTAIDTLKSHGVKVVIGGMYEYG
                     LSRYFTAMLARKGDYPGDVTPAGYYFDQDVVTKSGILKEGRLEFRPPLVDITQLQPY"
     gene            complement(8273..9751)
                     /gene="menE"
                     /locus_tag="P6L01_001329"
     CDS             complement(8273..9751)
                     /gene="menE"
                     /locus_tag="P6L01_001329"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDP3030403.1"
                     /translation="MDFWLYKQAQQNGHHIAITDGQESYTYQNLYCEASLLAKRLKAY
                     QQSRVGLYIDNSIQSIILIHACWLANIEIAMINTRLTPNEMTNQMRSIDVQLIFCTLP
                     LELRGFQIVSLDDIEFAGRDITTNGLLDNTMGIQYDTSNETVVPKESPSNILNTSFNL
                     DDIASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLS
                     VLLRAVIEGFTVRIVDKFNAEQILTMIKNERITHISLVPQTLNWLMQQGLHEPYNLQK
                     ILLGGAKLSATMIETALQYNLPIYNSFGMTETCSQFLTATPEMLHARPDTVGMPSANV
                     DVKIKNPNKEGHGELMIKGANVMNGYLYPTDLTGTFENGYFNTGDIAEIDHEGYVMIY
                     DRRKDLIISGGENIYPYQIETVAKQFPGISDAVCVGHPDDTWGQVPKLYFVSESDISK
                     AQLIAYLSKHLAKYKVPKHFEKVDTLPYTSTGKLQRNKLYRG"
     gene            complement(9910..10365)
                     /locus_tag="P6L01_001330"
     CDS             complement(9910..10365)
                     /locus_tag="P6L01_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4909 domain-containing protein"
                     /protein_id="HDP3030404.1"
                     /translation="MKICLMLYLLIFLSSCGTNNKIVGQRFEIQQHNETIGSIYFSAD
                     YAHIRGIEKGTAKYFIDKVGSKRYLFIEYIPDNVLNCKPDFWKTLKYKKDKVTYYVYL
                     IENLDDEVFHLSALQDMNRIPIDIADDVATMGKSPHQNDRMTLKLNKNN"
     gene            10668..11318
                     /locus_tag="P6L01_001331"
     CDS             10668..11318
                     /locus_tag="P6L01_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001795210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HDP3030405.1"
                     /translation="MKITYKYRGDLPLNTENNKNQNQSVKNSERRGMLKGCGGCLISF
                     ILLIILLSACSMMFSNNDNSTSNQSSKTQLTQKDENKNEDKPEEKSETATDEDLQSTE
                     EVPANENTENNQHEIDEITTKDQSDDEINTPNVAEDKSQDDLKDDLKEKQQSSNHHQS
                     TQPKTSPSTETNRQQSFANCKQLRQVYPNGVTADHPAYRPHLDRDKDNRACEPDKN"
     gene            11399..12394
                     /locus_tag="P6L01_001332"
     CDS             11399..12394
                     /locus_tag="P6L01_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDP3030406.1"
                     /translation="MSNQFKSEEERRQWEQFQAFQNQQNQQHQQYGQKKSKKGWFWGC
                     GGCLVLFILIIIGISACTAGVTGNLGGNSSKEANKTHKIGETVKNGDLEVTVNSVETM
                     KSVGPSLAPTNAKGIFVVADVTIKNKGKEALTIDSSMFKLKSGDKTFEADNTGSMSAN
                     QSDNGSIENSFFLQRINPDSTAQGKIVFDVSENIANAKDKKLEVISSLFSVKKITFDL
                     SDAKKTSKAKKDKQDTEVAVASSNSDNVNYEASATTPATTSSADTDSEDSEKSSKDED
                     KQNASKSDKSSVEKSESNEETATVEPMPQSKPTTSEAPPSQNNHNENNMYDASTE"
BASE COUNT         4040 a         2349 c         1911 g         4099 t
ORIGIN      
        1 atttctatca aataattaat agaaatcaac ttttttacat tgcctaagaa cttaatgtcc
       61 caagccctga caattcatct aaccttttca tcagttgagt gattttaatc tatccttcaa
      121 aagtttttgg atcaggtcca attctcttat cttgatttaa accatcaatt cgcgtcattt
      181 gttcatctga taattcgaaa tcaaatattt ggaagttttc agagattctg tttggtgtta
      241 ccgatttagg gattataacc acaccatgct gcacattcca tcttaaaaca acttgggcag
      301 gtgactttcc taattcttga gcaatgtctt taattgtctc atcatttaaa atttgtgcat
      361 tcatcaatgg tgaccaagat tccatcacga tatgttgtgc tgccaaatat aatttcaatt
      421 tatgttgcgt taaatatgga tgatattcaa cttgattaat tacaggttta attgacactt
      481 gtgccaacaa agcttccaaa tgttcaggtt caaaattgct gacacctata tttttagctt
      541 tattattttt atataaatct tccatacctt tccatgtatc aaccattacg gcttcgttcg
      601 tgcctggcca atgtactaga tacaaatcta agtattttaa acctaatcta gataaactag
      661 cttcgtaagc agctgctaca ttttcacgac cgaaatcctc aaaatataat tttgaagtaa
      721 taaataagtc ttctctagca ataccagttg actccaatcc ggcacgaatg ccagcaccta
      781 cttgttcttc attcccataa acttttgcgg tatcaatact acgatatcct tgttcaatgg
      841 catacttaac actttccatg caattttcat cattttccac acgaaatgtc cctaaaccaa
      901 tttgtggcat cgtgtttcca ttataaaatg ttttaacctc cataaatatc gcctcacctt
      961 tttgatgtat tataccctgt tatcataaca aatctgagtt gaatacatga gaaaaaacac
     1021 ttagagcaat caaccactaa aattctagta atatctctca aacattaatc aaattgtaaa
     1081 agcaattatg tttaatttat gacaaactaa aaaagccgaa gtcacaacat atagtcatca
     1141 cttcagccta acatttaatt gaatgattca attttatcca tcatttgttg taagtcttcc
     1201 acgttgtatt gaatacgacc atggaataca aatttgttaa agaactcgtc taattgttca
     1261 gcaccgacaa gcactttgac agcactattt tgattataat ttgaaatcgt tacatcgcct
     1321 tcatttttag gattaaagta taaaattgaa gttggcgtat atttggcacc taattctttt
     1381 tgtaagtctt cagccaattg tttaatcgcc tcaatttgat ctgaataatt tacaaaagat
     1441 aatgaacgtt tgtcatcatt ttgatccatc acaatagttt gtggtctaga tttatctaaa
     1501 tccaatgtat caaatacttg ttccattggt ggtaaatctt taaattgacc gccactaata
     1561 ccattataaa catgaccttt taacaattga gaatcaataa tataaagacc agttcttgtt
     1621 aatactaaat gactaattcg ttcaatatta ttaaagccat cctttggtaa aaagatattt
     1681 gccataatgt gcatatcttc tggtcgaatt cgtttttctt taactaatct ttcacgaata
     1741 ccaattaatc tcatgtccgt tacatattca ctatgatttt tcgagaacaa ttttaatgcg
     1801 tcaatctcac gatcttttgt actaaccatg tgattataat cttcttgttg ttttgtaatt
     1861 gtctttttat tttgaatacg ctctttctct aaagcttctt catgagactt tttaatgttt
     1921 tgttcttgtt gttcatactt ttcttctgtt tgtcgcttaa cttttttctt actacctaag
     1981 gcaactaaga aaaggacaaa aaagattaat gcaatgacta ctgcaataat gagtccaatg
     2041 actaccggtg aagataaatc catcacaaca acgctccttt ttaatatatg aataacttta
     2101 attataatag aaaagctaaa gattttcgat acatattatc atttatatac cgaaaatctt
     2161 ttatttagct atattaaatt catcttaata ttttactgcg tcttttaatt cttccacttt
     2221 gtctaatttt tcccatggga ataatacatc tgtacgtcca aaatgaccat aagcagcagt
     2281 ttgtttgtaa atcggttgtt tcaaatcaag cattttaata atgcctgcag gtcttaggtc
     2341 aaagtgtttt ctaactgctt cgacaagttg tccttcagaa actttacctg ttccaaatgt
     2401 atcaattgca attgacactg gttctgcaac accaatcgca tatgccaatt gtacttcaca
     2461 ttgatctgct aaacctgctg caacaatatt tttagccaca taacgtgcag catatgcagc
     2521 tgagcggtct acttttgtag gatccttacc actgaagcat ccgccaccat gacgtgcata
     2581 gccaccatac gtatcaacaa tgattttacg tcctgttaat cctgcatcac cttgaggtcc
     2641 accgattaca aagcgtcctg taggattgat gtagaattta gtttgttcat taatcaagtt
     2701 ttctggaaca gttggataaa tgacatgcgc tttgatgtct tcttgaattt gttcaagtgt
     2761 cacatcatca gcatgttgtg ttgatacgac aatcgtatca atacgtactg ggttatcatt
     2821 ttcatcatat tcaacagtga cctgaacttt accgtctggt cgtaaataat ttaacgtacc
     2881 atctttacgc acatcagata aacgttttgc caattgatgt gataaataaa ttgctagagg
     2941 catgtacgtt tctgtttcat tcgttgcgta accaaacatt aaaccttggt cacctgcacc
     3001 tgttgcttca atttcttctt cgctatcttt atcacgatac tctaatgctt tatccacgcc
     3061 ttgtgcaatg tcaggtgatt gttcatcaat cgcagttaaa attgccattg tttcataatc
     3121 ataaccatat tttgctcttg tgtatccaat ttctttaatt gtttctctaa caactttcgg
     3181 aatatcaaca tatgttgttg tagaaatttc gccggcgatc aatgccatac ctgttgtaac
     3241 agttgtttca caagctacac gtgcatttgg gtcgtctttt aaaatagcat ctaatattgc
     3301 atctgacact tggtcagcga ttttatctgg gtgtccttct gtaacagact ctgaagtaaa
     3361 taatcgtttg ttatttaaca tagtttgctc ctttaaattt atattacgaa aattctctct
     3421 ctgtgagcta aataaaaaag accttctaac tattaatata gagagaaggc ctaatacgtc
     3481 cattcgctct tatcgttcag acctatttgt ctgcaaacgg tttggcacct ttcttttata
     3541 aaaaaagagg ttgctgggtt tcattgggtc catgtccctc caccactcag gataagagaa
     3601 tccgttaaaa ataatagtac ctaattaatg aattaatgtc aatttttcac aaataaattt
     3661 acagtaaaat attgtagatt aattatgtta atgtgttata ctaattaaat gtaaaggctt
     3721 acatttaaat tatcgctttg gagggattta ggatgtcagt agacacatac actgaaacaa
     3781 ctaaaattga caaattactg aaaaaaccaa cgtcacattt tcaactttcg acgacacaac
     3841 tttataataa aatcttagac aataacgaag gggtattaac agaacttggt gctgttaatg
     3901 caagtactgg aaaatatact ggtcgttcgc ctaaagacaa attttttgtc tctgaacctt
     3961 catatagaga taacattgat tggggagaaa ttaatcaacc tatcgatgaa gaaactttct
     4021 tgaagttata ccataaagta ctagactatt tagataaaaa agatgaacta tacgtattta
     4081 aaggctacgc tggtagcgat aaagatacaa tgttaaaact tacagtcatc aatgaattag
     4141 catggcataa tttatttgct aaaaatatgt ttattagacc tgaatcaaaa gaagaagcta
     4201 caaagattaa acctaacttc actatcgttt ctgcaccaca ttttaaagca gatccagaag
     4261 ttgatggtac taaatctgaa acctttgtca ttatttcatt taaacacaaa gtcattttaa
     4321 tcggcggtac tgaatacgct ggtgaaatga aaaaaggtat cttctctgta atgaattatc
     4381 tcttaccgat gcaagatatt atgagcatgc attgctcagc aaacgttggt gaaaaaggcg
     4441 atgttgcatt attctttggt ctatctggca ctggtaaaac aaccttatcg gctgacccac
     4501 accgtaaact aatcggtgat gatgaacacg gatggaataa aaacggggtc tttaatatcg
     4561 aaggtggctg ctatgcaaaa gcaattaatc tttccaaaga aaaagaacca cagatttttg
     4621 acgcaatcaa atatggtgca attttagaga acactgtagt tgcagaagat ggttcagtgg
     4681 actttgaaga caatcgttat acagaaaaca cgcgtgccgc ttatccaatt aatcacattg
     4741 acaatattgt agtaccatca aaagcagcac atccaaatac aattattttc ttaactgcgg
     4801 atgcatttgg tgttattcca ccgatttcaa agttaaataa agaccaagca atgtatcatt
     4861 tcttgagtgg tttcacttct aaattagctg gtacagagcg tggtgtgaca gaacctgaac
     4921 catcattctc aacatgtttc ggagcaccgt tcttcccgtt acaccctact gtttacgctg
     4981 atttattagg tgaacttatc gatttacatg atgttgatgt ttatcttgtt aatactggat
     5041 ggactggcgg aaaatatggt gtaggacgta gaatcagctt acattacaca cgtcaaatgg
     5101 taaaccaagc gatttctggc aaattgaaaa atgcagaata tacaaaagat agtacgtttg
     5161 gtttaagcat tcctgtagaa attgaagatg taccgaaaac aattttaaat ccaattaatg
     5221 cttggagcga caaagagaaa tataaagcac aagcagaaga tttaattcaa cgttttgaaa
     5281 agaacttcga aaaatttggt gaaaaagttg aacatattgc tgaaaaaggt agcttcaaca
     5341 aataaatttg aatactaatt aaagagccaa ccgttgttat agtctaacaa tggttggctc
     5401 ctcttatttt atgtgctaaa aatttatagg caattttatt acaacaatgc acatttaagg
     5461 tgaccttcat gccaaactcg catcactcat ttaatggaag cagcacgtct tcacataaag
     5521 taccggttcc ttacgctatg catgttgccc cactttgcat aaagtactta tcgttaactc
     5581 aacgcaagtt gttcctattc acaaaaagca catacagtta tctgcatgga gcacattttc
     5641 attgaaagcg catacccctc attcaacgta tatcacacat cttcaaagct tgatagttcc
     5701 catgcgcaca ccacgtttca tactagctat gcaactcaac ttgtttcata aactctttaa
     5761 tataagtcaa tgtttcaacc atcgctggtg gtcttggcac atgtccttct gccatttgat
     5821 aaaatgtttc atgcgtggca ccttttaact ctagttggtc cgctaaataa tacgcatgat
     5881 gaataccaac ttgctggtct ttccctccat gtacaattaa tattggcgga ctgttttcat
     5941 taatgtttgg aatcgcttgg cgtgcctcat atgccgctcg atcttttttc ggatgaccaa
     6001 tcattcttcg tagcatgcct cttaaatcga cacgttcttc atacattaaa tcaatatctg
     6061 agacaccacc ccagattgta taacttgtta ctggtaagtc ttgaaatgtc aacaatcctt
     6121 gtaaaccacc tcgcgaaaaa ccaaccatgt ggataaatgc atgtggatat ttatcatgta
     6181 gcaaccttaa taattgcgtc acatcattta aatcgccacg gtaaaattcg tctttacctt
     6241 cactcccatt gttacctcgg tagtatggcc caatcactaa agtttgacta tctgaaaatt
     6301 gcattaatct acctgcgcgc acacgtccta cttgaccttt gccacctcgc aaataaacta
     6361 caatgcgatt tacttcatga tgtggtgtca tcattaaagc ttttacttgt aagtcatctg
     6421 acaaatatgt aatttcttcg aattgatgcg taaaagattc aattggcatt cgtttacgtt
     6481 tgataaaatc caagtgattg caccctctct acgcatttta aaatggtact atcttgcagt
     6541 aagaaactcc gttgtgcgag ttcaatatca ttgatacagt taaacaacac tggccctgct
     6601 gtttctaaat aatcgttctt gcttaccaat gattcaactt cgataaaata tacatctttt
     6661 acaaaatcag tttgatcatg tgtttcaatg gtatattgtg ctatgtaatg aatattttta
     6721 actttggcgc ctgtttcttc atataattca cgggtaactg cttcagcact actttccccg
     6781 cgttcccttt taccaccagg aaattcaatc ccccgtaaat tatgtttggt aaaaagcaat
     6841 tgatttttaa acgttggaat agctagcaca tgattgccat ctgctatctc attatccttt
     6901 ttaaatgtca aattaacttg acgattatct ttatccctaa acttcacgcg catcacatcc
     6961 ctacattgta tgttaatata atagttaatt actatcgttg gaggcattaa ttatgaaaaa
     7021 gatattcttg gcgatgattc atttttatca acgtttcatt tcgccactca ctccaccaac
     7081 ttgtcgtttt tatccaacat gttcagagta cactagagaa gcgattcaat accacggtgc
     7141 tttcaaaggc ctttatttag gcatccgtcg tattttaaaa tgtcatccgc ttcataaagg
     7201 tggctttgac cctgttccgt taaaaaaaga caagtcagca agcaagcatt cacataaaca
     7261 taaccattaa tatggttgta attgagttat atccactaaa ggggggcgaa attcgagtcg
     7321 cccctctttt aatatgcctg atttagtaac cacatcttga tcaaaataat aacctgctgg
     7381 tgtaacatct cctggataat cacctttacg agcaagcatc gctgtaaaat aacggcttaa
     7441 accatattcg tacatgccgc caataaccac ttttacacca tgacttttca aagtatcaat
     7501 tgccgtttgc actttatcaa tgccacctag acgaaatggt tttaatacaa caactttcac
     7561 attgtataat tctatcaaat taattatgtc caacaacgat gttgcctttt catcaagggc
     7621 tattggaggt attgttccat ccactacttc atcaagcatg gagatatctt taaatggctc
     7681 ttcgatataa agaacctgtt cacgcgctaa tagctgtaac tgtgtgaaat cttgacgatc
     7741 caatgactca tttgcatcta taaccaattg aaagtgaaag tctaattccc gtaacactct
     7801 aatttgatgc atgatttgag gcgtccattt taatttaatt ctggtcggct ttgttgcttt
     7861 caatgactct agttgtttat ttgataagcc gctcgctgtc gctccatatg ctactgaaaa
     7921 tgaaggcagt acatgaaaca tttgatacaa tgccatgaca atagttgccc ttgcagcagg
     7981 cgtattttcc aatgaatcta ctaattttag tgctgcttca tacgtttcaa atgatttatt
     8041 tctattatct tcgaaccatt gctcaattac atgtttcact gaagcaattg tttcatgatc
     8101 ataccaatct gtttgaaaag cgttacattc cccgaaatat gcatttcctt tgtcatcaat
     8161 caattcgata aacaaacaat cacgatgcgt taaagtgact ttcggtgtta caatttgtga
     8221 cttaaatggc tcactatatt tataaaaatg caaagctgtc aacttcatca aatcatcctc
     8281 tatacaactt atttctctgt aatttacccg ttgatgtata aggtaaagta tcaacctttt
     8341 caaagtgttt cggtacttta tatttcgcta aatgttttga taaatatgca atcaattgtg
     8401 cctttgaaat gtcactttca ctgacaaaat ataatttagg cacttggccc caagtatcat
     8461 caggatgccc tacacatact gcgtcactga tacctggaaa ttgcttcgct accgtttcaa
     8521 tttgatatgg ataaatattt tcaccgccac taataattaa atctttacgt cggtcataaa
     8581 tcataacata accttcatga tctatttcag caatgtcacc tgtattaaaa taaccatttt
     8641 caaacgtacc cgttaaatct gttggataca aatatccatt catcacattg gcacctttaa
     8701 tcattaattc tccatgacct tctttattag gatttttaat tttaacgtca acattggcac
     8761 ttggcatccc tacagtgtca ggacgtgcat gcaacatttc cggtgttgct gttaaaaatt
     8821 gcgaacatgt ctcagtcata ccaaatgaat tataaattgg caggttatat tgtaatgccg
     8881 tctctatcat agtggcagat aatttagcac cgccgagtaa tattttttgt aaattataag
     8941 gttcatgtaa accttgttgc ataagccaat ttaaagtttg tggcacaagc gaaatgtgcg
     9001 tgattcgttc atttttaatc atcgttaaaa tttgttcggc attgaattta tcaacaatgc
     9061 gcacagtaaa accttcaata acagctctta aaagtacact gagacccgaa atatgataaa
     9121 tcggcaagac agatagccaa ttagtgtcac gatcaaatcc caagctctct ttacatccga
     9181 ttgcactggc ataatgatta cgaaacgttt gtggcaccgc tttttgaggg cccgttgtcc
     9241 ctgatgtaaa cataattgat gcaatgtcat ctaaattaaa tgatgtattt aatatgttgg
     9301 acggcgactc tttcggcacc acagtttcat tcgatgtatc atattggata cccattgtgt
     9361 tgtccaacaa accgttcgtt gtaatatccc ttccagcgaa ttcaatatca tccagcgata
     9421 caatttgaaa ccctcgcaat tccagtggca aggtacaaaa aatcaattgt acatcgattg
     9481 acctcatctg attcgtcatc tcattaggtg tcaaccttgt attaatcatc gcaatttcaa
     9541 tatttgccaa ccaacatgca tgtattaaaa tgatcgattg aatcgaatta tctatgtata
     9601 gcccgacacg agattgttga taagccttga gtcttttagc caatagactc gcttcacagt
     9661 ataaattttg ataagtataa gattcttgac cgtctgttat cgcaatatga tgtccatttt
     9721 gttgtgcttg tttatataac caaaagtcca tgcgttattc ctccaaaatc atttacatta
     9781 taattataac gattttatga cattctagca gtggttatgt ttaaaaatat aaaaaagtag
     9841 acgaattgat tcattgatat gattgttata atgctcaata catatcgtta tatcattcgt
     9901 ctactattat cagttatttt tatttaattt tagtgtcatt ctgtcatttt gatgtggtga
     9961 tttacccatt gttgccacat catctgcaat gtcaattggt atacgattca tgtcttgtaa
    10021 tgcacttaaa tggaatactt catcatctaa attttcaatg agatatacat aatatgttac
    10081 cttgtccttt ttatatttta acgttttcca aaagtccggc ttgcaattca atacattatc
    10141 cggaatatat tcaataaata agtaacgttt gctgcctact ttgtctatga aatattttgc
    10201 agtgcctttt tctatacctc ttatatgtgc atagtctgct gaaaagtaaa tactacctat
    10261 tgtttcatta tgttgttgta tttcaaatcg ttggcctact attttattat ttgtgccaca
    10321 actacttaaa aaaatcagta gatatagcat taaacatatt ttcatcccct tgaattttaa
    10381 aaactttttc aaaagcaaca cctctaaaaa taaatacaat atattatagc aaatagaaat
    10441 tattattttg ttaaattatg ttgtcatgtt cgtgtcattt tgagtcgtaa tacaacttta
    10501 caattattaa attaacactt ctacaatcca aaaatatttt taaagttttg aaaaaaatta
    10561 aagtatttcc actactaatt tggtaaaata tgattatgtg atatagtgaa agcgttttac
    10621 acttaataac tccctcttaa atgcatccag gttttatgta gtaaatcatg aaaataacat
    10681 ataaatatag aggagattta cctttgaata cagagaacaa caagaatcaa aaccaatctg
    10741 ttaaaaattc tgaaagacgc ggcatgttaa aaggatgcgg cggttgcctt atttctttta
    10801 ttttattaat aatcttatta tcagcctgtt caatgatgtt tagtaataat gacaattcca
    10861 ctagtaatca atcatcaaaa acgcaattaa ctcaaaaaga tgaaaataaa aatgaagata
    10921 agcctgagga aaaatcagaa acagctacag atgaggattt acaatcaacc gaagaagtac
    10981 ccgcaaatga aaatactgaa aataatcaac atgaaattga tgaaataaca acaaaagatc
    11041 aatcagatga tgaaattaac acaccaaacg ttgcagaaga taaatcacaa gacgacttga
    11101 aagatgattt aaaagaaaag caacaatcaa gtaaccatca tcaatccacg caacctaaga
    11161 cctcaccatc aactgaaaca aacaggcaac aatcatttgc taattgtaag caacttagac
    11221 aagtttatcc gaatggtgtc actgccgatc atccagcata tcgtccacat ttagatagag
    11281 ataaagataa tcgtgcatgt gaacctgata aaaattaaac aacaagcgaa ttgaattcaa
    11341 attgtattta gctttatgca ctaatcacat agtaaataat gagggagatt ttttaggcat
    11401 gagcaatcaa ttcaaaagtg aagaagaacg aagacaatgg gaacaattcc aagctttcca
    11461 aaatcaacaa aaccaacagc accagcaata cggacaaaag aaatctaaaa aaggatggtt
    11521 ctggggttgt ggtggttgtc tagtattatt tattttaatt atcatcggta tttcagcttg
    11581 tacagctggt gtcactggta accttggtgg aaatagttct aaagaagcga acaaaaccca
    11641 taaaatcggt gaaactgtta aaaatggcga ccttgaagtc actgtaaatt cagtggaaac
    11701 tatgaaatct gtaggaccgt ctcttgcacc aacaaacgct aaaggtatat ttgtcgttgc
    11761 tgatgtaacg attaaaaaca aaggtaaaga agcgttaaca attgatagtt caatgtttaa
    11821 gttaaaatcc ggtgataaaa catttgaagc agataataca ggttcaatgt ctgctaatca
    11881 aagtgacaat ggtagtatag aaaattcatt tttcttacag cgtataaatc ctgatagcac
    11941 tgcacaaggt aaaattgttt tcgatgtgtc agaaaacata gccaacgcaa aagataaaaa
    12001 attagaagtt atttctagtt tatttagcgt caagaagatt acatttgatt tatccgatgc
    12061 taaaaaaaca tcaaaagcta aaaaagacaa gcaagataca gaagtagctg ttgcgagttc
    12121 aaatagcgat aatgtaaatt atgaagcttc ggctactaca cctgctacaa cttctagtgc
    12181 ggatactgat tctgaagata gcgaaaagtc tagtaaagat gaggataaac agaatgcgtc
    12241 taaaagtgat aaatctagtg tagaaaaaag tgaatctaat gaggaaactg ctactgtaga
    12301 gcccatgccc cagagcaaac ctaccactag tgaagcacca cctagtcaaa ataatcacaa
    12361 cgaaaataac atgtatgatg cttcaacaga ataaatttt
//
LOCUS       DAOBBH010000066        12202 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.066, whole genome shotgun sequence.
ACCESSION   DAOBBH010000066 DAOBBH010000000
VERSION     DAOBBH010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12202
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.066"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            30..1448
                     /locus_tag="P6L01_001333"
     CDS             30..1448
                     /locus_tag="P6L01_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDP3030407.1"
                     /translation="MENTVKYRKFILPIVVGLLIWALTPFKPDAVDPTAWYMFAIFVA
                     TIIACITQPMPIGAVSIIGFTIMVLVGIVDMKTAVAGFGNNSIWLIAMAFFISRGFVK
                     TGLGRRIALHFVKLFGKKTLGLAYSIVGVDLILAPATPSNTARAGGIMFPIIKSLSES
                     FGSKPKDGSARKMGAFLVFTEFQGNLITAAMFLTAMAGNPLAQNLASSTSNVHITWMN
                     WFLAALVPGLVSLIVVPFIIYKIYPPTVKETPNAKSWAENELATMGKIALAEKFMIGI
                     FVIALTLWIVGSFIHIDATLTAFIALALLLLTGVLTWQDILNETGAWNTLVWFSVLVL
                     MADQLNKLGFIPWLSKSIATSLGGLSWPIVLVILILFYFYSHYLFASSTAHISAMYAA
                     LLGVAIAAGAPPLFSALMLGFFGNLLASTTHYSSGPAPILFSSGYVTQKRWWTMNLIL
                     GFVYFIIWIGLGSLWMKVIGIF"
     gene            complement(1862..2770)
                     /gene="rarD"
                     /locus_tag="P6L01_001334"
     CDS             complement(1862..2770)
                     /gene="rarD"
                     /locus_tag="P6L01_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDP3030408.1"
                     /translation="MNIEYKKGIFLALSAYILWGILPIYWQFVDAIGAFEILAFRIIF
                     SAIFMIFILAVGQKQRNAFQRDMNQLLGKPIQLLAIVVAGYVITLNWGTFIWAVTNGH
                     VLQTSLGYYINPLVSILLALIFLKERFNKFEWLAILFAFIGVLYMTLKIGEFPIVSII
                     LALSFGTYGLLKKVVHIDAISSITIECIVTAPAGLIYVIYLWQQHQMSFGLNMSSFWL
                     LFSGAITAIPLILFSAGAKRIPLSLIGFIQYVGPTIMFVLGIFVFKEPFSIDQLITFI
                     FIWTGIVLYSLSQYIKLKKHPVAKTL"
     gene            2992..3567
                     /locus_tag="P6L01_001335"
     CDS             2992..3567
                     /locus_tag="P6L01_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDP3030409.1"
                     /translation="MTLPKIGKPATRALNSQGIYTLEAVSQYTKSSLMEMHGVGPKAI
                     SILEQALFQHQLHFKTEVQSSLPFKLTGDVSCNHAPKRQQMIDFIVATAALDIELLRS
                     LVTTEFIWSVPGHFDIYGPQILIQELSNYYNQVASLNIHSNITHGCLGSMHGIEILKT
                     GKEIHFAHFFEFENHKKDAKLSKVTSYIVVA"
     gene            3729..4745
                     /locus_tag="P6L01_001336"
     CDS             3729..4745
                     /locus_tag="P6L01_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HDP3030410.1"
                     /translation="MTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGI
                     LAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGIS
                     VKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEV
                     DVLLESRGFVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAI
                     SFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALV
                     IGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEH
                     KWSK"
     gene            4988..5782
                     /locus_tag="P6L01_001337"
     CDS             4988..5782
                     /locus_tag="P6L01_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HDP3030411.1"
                     /translation="MNIAKLENYLQIDSSRYNRPSIEALNYYATRFMLTVPFENIDVQ
                     NSKPISINIDALFNKIVHDKRGGFCYELNTFFKAYLQQKGFNPELMSATIHTPGGGRS
                     LNGSHASLVVSINDVFYVTDVGFGDLPLHAIPITSSEHTQPITDISGTFRAIFNNEDK
                     DIFYVQKFENDHWHTKYEAEFKPKQIEDFNSNIEYNQTHPDSIFVQHLLITMPQSFGR
                     ATMSENHLTLTRNGSSEKFDVTKDNYKHFLEKYFGLNVTINRIEKQ"
     gene            complement(5916..6428)
                     /locus_tag="P6L01_001338"
     CDS             complement(5916..6428)
                     /locus_tag="P6L01_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HDP3030412.1"
                     /translation="MEKGNQGIKWSSLIMGVLLLMLAVVIFTFPIENFYAITWLIGLF
                     VLINGVIQIIYRRKAKALVGGNQNWILFMGIVDILFGLIVIFNVGASSAFFIYMFAFW
                     FIFSSIAGLFTFTGSGSLKLISVIFNLLGIVFGVILLFNPLMGIVFISTMIAIAFVFV
                     GVIYVVDALA"
     gene            6711..7469
                     /gene="vraD"
                     /locus_tag="P6L01_001339"
     CDS             6711..7469
                     /gene="vraD"
                     /locus_tag="P6L01_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter ATP-binding
                     subunit VraD"
                     /protein_id="HDP3030413.1"
                     /translation="MTILSVQHVSKTYGKKHTFQALKDINFDIQKGEFVAIMGPSGSG
                     KTTLLNVLSSIDQISSGSVIANGQELNKLNQKALAKFRKESLGFIFQDYSILPTLTVK
                     ENIMLPLSVQKMSKATMEENYKAITTALGIYDLGNKYPSELSGGQQQRTAAARAFVHK
                     PQIIFADEPTGALDSKSANDLLQRLEEMNKSFDTTIVMVTHDPVAASFAERVIMLKDG
                     QIHTQLYQEGRSKQAFYEDIVHLQSVLGGVSNDI"
     gene            7459..9339
                     /gene="vraE"
                     /locus_tag="P6L01_001340"
     CDS             7459..9339
                     /gene="vraE"
                     /locus_tag="P6L01_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter permease
                     subunit VraE"
                     /protein_id="HDP3030414.1"
                     /translation="MTFNHIVFKNLRQNLKHYAMYLFSLFFSIVLYFSFTTLQFTKGV
                     NNDDSMAIIKKGALVGSIFLFIIIVIFLMYANHLFVKRRTREFALFQLIGLTRQNILK
                     MLALEQMIVFLITGVVGVLCGIAGAQLLLSIVSKLMSLSINLSIHFEPMALVLTIFML
                     IIAYVLILFQSALFLKRRSILSMMKDSIKTDATTAKVTTAEVISGVLGIAMIALGYYM
                     ATEMFGTFKALTMAMTSPFIILFLTVVGAYLFFRSSVSLIFKTLKKSKNGRVSITDVV
                     FTSSIMYRMKKNAMSLTIIAIISAVTVTVLCFAALSKSNTDQTLTSMAPNEFNVVATQ
                     DAKQFETKLSQQQITFSKNAYETITVDNVKDQVITLENGSDSGRTNSILSANNKVTGN
                     NAIITNTKSLPNIINIHLNKDLVVKGTKNETFRVTQEDKGRVYPLNLSFNSPVVEVSP
                     EKYQQLKTQNNVHTFYGYDIKQTSQKEKAQAIAKQFGDKVITYDEMKKEVDATNGILI
                     FVTSFLGLAFLVAAGCIIYIKQMDETEDELSNFRILKRIGFTHTDMLKGLLLKITFNF
                     GLPLLIAILHAVFAAIAFMKLMGNISFMPVIVVIVVYTLIYITFALIAFVHSNKLIKK
                     TI"
     gene            9431..9622
                     /gene="vraH"
                     /locus_tag="P6L01_001341"
     CDS             9431..9622
                     /gene="vraH"
                     /locus_tag="P6L01_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter activity
                     modulator VraH"
                     /protein_id="HDP3030415.1"
                     /translation="MKFRELVKQSYEDLKNLTVTWYNVIALAMIVIVLSVVTTPIIGI
                     PAGLLGGAYYLKRREEKGK"
     gene            10014..10214
                     /locus_tag="P6L01_001342"
     CDS             10014..10214
                     /locus_tag="P6L01_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDP3030416.1"
                     /translation="MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEG
                     QKVTFEITEGQRGDQAVNVQTV"
     gene            complement(10332..10901)
                     /locus_tag="P6L01_001343"
     CDS             complement(10332..10901)
                     /locus_tag="P6L01_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDP3030417.1"
                     /translation="MNLSKQIKKYRERDGYSQEYLAEKLYVSRQSISNWENDKSLPDI
                     HNLLMMCELFNVTLDDLVKGTIPFVPDIKVQRSLNLWTYVMLIFMTLAAILMGPLVVY
                     WNWTWGITVAIILGIGFYASMKIEDLKKVHKMDNYDRIVAFMNGKDPSEVQTTKARNT
                     MTNALSIISAIGILSLIIFLSVYMANKFL"
     gene            complement(11161..11556)
                     /locus_tag="P6L01_001344"
     CDS             complement(11161..11556)
                     /locus_tag="P6L01_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HDP3030418.1"
                     /translation="MKLTLMKFFVGGFAVLLSYIVSVTLPWKEFGGIFATFPAVFLVS
                     MFITGMQYGDKVAVHVSRGAVFGMTGVLVCILVTWMMLHMTHMWLISIIVGFLSWFIS
                     AVCIFEAVEFIAQKRLEKHSWKAGKSNSK"
     gene            complement(11624..11977)
                     /locus_tag="P6L01_001345"
     CDS             complement(11624..11977)
                     /locus_tag="P6L01_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HDP3030419.1"
                     /translation="MFSIGSAILHFVIGGIAVALASIIADKVGGKLGGIIATMPAVFL
                     AAIIALALDHRGTQLVEMSMNLSTGAIVGILSCILTVFLTSLYIKHKGYRKGAIFTVV
                     CWFVISLAIFSIRHL"
BASE COUNT         4123 a         2134 c         1890 g         4055 t
ORIGIN      
        1 ataaaacata gaaagaagga cgttttaaca tggaaaacac ggttaaatat cgtaagttta
       61 tactccctat cgttgtaggt ctccttattt gggcacttac accttttaaa ccggatgctg
      121 tggatccaac agcatggtat atgttcgcaa tattcgtcgc gacaatcatt gcttgtatta
      181 cacaaccgat gccaattggg gccgtctcta taattggatt tacaatcatg gtactcgttg
      241 gcattgttga catgaaaacg gctgtcgctg gttttggtaa taatagcatt tggttaattg
      301 ctatggcatt tttcatttcg agaggatttg tgaaaacagg tcttggtaga cggatcgcac
      361 ttcatttcgt caaattattt ggtaaaaaaa cattaggctt agcatattct atcgtcggtg
      421 tagatttaat tctagcgcct gctacaccga gtaataccgc gcgtgctggt ggaatcatgt
      481 tcccaattat caaatcactt tctgaatcat ttggttcgaa accgaaagac ggatcagcac
      541 gcaaaatggg tgcatttctt gttttcacag aattccaagg taatttaatt actgcggcta
      601 tgtttttaac tgcaatggcc ggtaaccccc ttgcacaaaa tttagcatct agcacatcta
      661 atgttcacat tacatggatg aattggttcc tagctgcttt agttcctgga cttgtttcct
      721 taattgttgt accttttatt atttataaaa tttatccacc aactgttaaa gaaacaccaa
      781 atgctaagag ttgggctgaa aatgaattag cgactatggg taaaatcgct ttagctgaaa
      841 aatttatgat tggtattttt gtcattgctc taacattatg gattgtcgga agtttcattc
      901 atattgatgc aactttaacg gcctttattg cgctagcatt gttattattg acaggtgtct
      961 taacatggca agacatttta aacgaaacag gtgcttggaa cacattagta tggttctcag
     1021 tattagtgtt aatggccgac caattaaaca agcttggatt tattccttgg ttaagtaaat
     1081 ccattgctac aagtcttggt ggcttaagct ggcctatagt actggtcatt ttaatattgt
     1141 tctacttcta ttcacattac ttatttgcaa gttctacagc acatatcagt gcgatgtatg
     1201 cagcattact aggcgttgcc atcgcagccg gtgcaccacc attattcagt gcattaatgt
     1261 taggtttctt cggtaaccta ttagcttcaa caacacacta tagtagtggt ccagcgccga
     1321 ttctattctc ttcaggttac gtgactcaaa aacgttggtg gacaatgaac ttgatcttag
     1381 gtttcgtcta ctttattatc tggattggtt taggatcact ttggatgaaa gtaattggta
     1441 tattttaaaa tatttaaatt agtgctcgaa tctcattgat ttgggcgctt tttaatttgt
     1501 atttaaaatc aacctttgct aaatcaagac tcccttttta aaatacgttt atcctttaaa
     1561 tcattgcgtg cttcactgaa aatttgtata aagatttaag tcattacgta acatcacata
     1621 aaatacattt ctatactatt ccgtttcatt gatcaacatt acgtatgccc tcataaatca
     1681 tgaaataaaa aaacaccttc gtttaaattc attttaagtg cgaattcaac gaaagtgatt
     1741 tatttcatat tcaatgtttt aaatttatac agctgtcact gttactgcac acatacctca
     1801 gtctcacctt atcatgacac aaaggatatt gaaaacacgt ataattatct ttcctcgtat
     1861 tttatagggt ttttgcgacc ggatgtttct tcaatttaat gtattgagaa agactatata
     1921 acacaatacc tgtccaaata aatataaacg taattaattg atctatacta aaaggctctt
     1981 tgaaaacaaa tatgccgagt acaaacatta ttgttggtcc aacgtattga ataaatccta
     2041 ttagcgaaag tggaatacgt tttgccccgg ctgagaatag gattagtggt attgccgtaa
     2101 tagcaccaga aaataacaac caaaatgatg acatgttcaa tccaaatgac atctgatgtt
     2161 gctgccataa ataaataacg tatattagtc cagcaggtgc tgtaacaata cattcaatcg
     2221 taatactgct gatggcatca atatgtacta cttttttcaa taatccatat gtaccaaagg
     2281 ataacgctaa tataatagaa acgattggga attctccaat cttgagcgtc atatataata
     2341 caccgatgaa tgcgaataaa atggctagcc attcaaattt attgaatctt tcttttaaaa
     2401 agataagtgc gagcaaaatg ctaacaagtg gatttatata ataacctaaa cttgtttgta
     2461 ggacgtgacc gttcgttaca gcccaaataa atgtacccca atttaatgta atgacatagc
     2521 ctgctacgac aatcgctaat agctgaatgg gcttgcctaa caattgattc atatctcgtt
     2581 gaaatgcatt gcgttgtttt tgtccaaccg cgagtatgaa tatcatgaat attgctgaaa
     2641 atataatacg aaaggctaaa atttcaaatg cgcctattgc atcaacgaac tgccaatata
     2701 taggtagtat tccccacaga atgtatgcac tgagtgctaa aaatatacct tttttatatt
     2761 ctatattcac ttacatacct ccttactctc ccaaattttt aacatgttac gctcacttgg
     2821 ttatgctaat ataacgattt tactaataat atttcgataa agatatcatt ttgtttatat
     2881 ttcccacatt tattcaccaa ccactaaaca atattaattt tataaataat tctgtacaaa
     2941 tcagggtata ttgccagaaa gactaccata caacataaag gatggataca aatgacttta
     3001 cctaaaattg gaaagcctgc aacacgcgcg ctaaattcac aaggtatata cacattagaa
     3061 gcagtatcac aatatacgaa gtcatctcta atggagatgc atggcgttgg tcctaaagct
     3121 atatcaatat tggaacaagc tttatttcag caccaactac attttaaaac ggaagttcag
     3181 tcatcattac cttttaagtt aacaggagat gtgtcatgca atcatgcgcc taaacgtcaa
     3241 caaatgatag attttatagt tgctacagca gcattagata ttgaattatt acgttcactg
     3301 gttacaacag aatttatttg gtctgtgcct ggacactttg acatttatgg acctcaaatt
     3361 ttaattcaag aactatctaa ctattacaat caggttgcta gtctcaatat ccactccaac
     3421 atcacacatg ggtgcttagg ttccatgcat ggtattgaaa tattaaaaac tggtaaagag
     3481 attcattttg ctcatttttt tgaatttgaa aatcataaaa aggatgccaa gttaagcaaa
     3541 gtgacatctt acattgttgt tgcttaatga ctcataatct ataacgctaa aaactatcgt
     3601 aattacatat atatgttaat aaaacaaata attttgtata atatttttgt tatatgaatg
     3661 ctttttacta gagtgcacat tgaacataca gtatgattaa atatcatgtt atgaaagaag
     3721 gatatatttt gactgttttt aaaaatgagc gcttaagctg gttaccatac atagctattg
     3781 tcattttgtt acacgttatt gggtttagtt ttttatggat tgctggaaaa gaccatcata
     3841 tcttatttgg tatggggatt cttgcataca cattaggttt gcgtcatgca tttgatgcag
     3901 atcatatcgc cgcgatagac aataccgttc gtaaattatt acaacaacgc aaagatccat
     3961 ctggtgtggg tttctatttt tcaattggac attcatctgt tgtattttta atggctgttt
     4021 ttttagggat atctgtaaaa tgggctaaag atgaattacc acatttccaa gatattggtg
     4081 gaacgattgg tacactagtt tcaggtttct ttttagtgct tatcggtgtg ttgaatctaa
     4141 ttattttaat ctctttaatt aacttgttcg ctaaattacg tcgtgaacac attgaagaag
     4201 ctgaagtcga tgtattactt gaatctagag gatttgtttc tcgatttgta ggaccttatt
     4261 tcaagttaat cacgcgtagt tggcacgtat tgccacttgg ctttttattt ggacttggtt
     4321 ttgatacagc tagtgaaatt gcgttactcg ctctttcttc aggcgcatca caacaagcca
     4381 tttcatttat cggaatctta tctttaccaa ttttattcgc atcaggtatg agtttattgg
     4441 atacattaga tggtgttgta atgaagtatg cctataattg ggcattcttc aatcctattc
     4501 gcaaaatcta ttacaatata acgattactg cgatatcagt catggcagca ttagtgattg
     4561 ggatgattga attgctacaa attcttgctg ataagctaga tttacatggt gcgttttggg
     4621 cattcattgg ttcgatcgaa tttgattatt taggctatat tttagttgca ttatttttaa
     4681 ttacttggct tatttcaagt ttaatttgga agtttggtcg catagaacac aaatggtcta
     4741 aataaagtaa cttgtcttgc aactaccttt gtattgttgc atttttagac accctgcaca
     4801 cttcttccat tatgctacgc tctcagtagt catctttaaa tcgtttcttt tagagatgac
     4861 ttcattttga gagcgtattt ttctatttaa aatgtattta tccaaaattc aatgctttcg
     4921 aatttaaaat ttcagcattt ctgtttaaaa tttaattata atctttaata caggaggcgc
     4981 tatgcttatg aatattgcga agttagagaa ttatttacaa attgattcat ctcgttataa
     5041 tcgaccgagt attgaagcat taaattatta tgcaacacgt tttatgttaa ctgtaccttt
     5101 tgaaaatatt gatgttcaaa atagtaagcc gatatctata aatatcgatg cactttttaa
     5161 taaaattgtt catgataaac gtggtggttt ttgttatgag ttaaatacat ttttcaaagc
     5221 atatttacaa caaaaaggat ttaatcctga attaatgtca gctactattc acacacccgg
     5281 tggcggtcgt agtctgaacg gttcacatgc ttcacttgtt gtttctataa atgatgtatt
     5341 ctatgtaact gatgttggct ttggagactt acctctacat gccattccta ttacttcatc
     5401 tgagcataca caaccaatta ctgatatcag tggtacattc cgcgctattt ttaacaacga
     5461 agacaaggat attttctatg ttcaaaaatt cgaaaatgat cattggcata caaaatatga
     5521 agctgaattc aaacctaaac aaatagaaga ttttaatagc aacatcgaat ataatcaaac
     5581 gcatccagat tcaatatttg tacagcattt attaattaca atgccacaat catttggccg
     5641 tgctacaatg tctgaaaatc atttaacttt aacaaggaat ggtagctcag aaaaatttga
     5701 tgtcactaaa gataactata aacatttttt agaaaaatat tttggactaa atgtaactat
     5761 taatcgtatt gaaaaacaat aatagtaaaa tataaaaata tgggcgtatc ttacttattg
     5821 taggtagcgc ccctttttta tgcttcataa acacaacaaa aaggatatga cacaaacttc
     5881 atgtcacacc cttcttttga accgcttcat tttacttaag caagtgcatc tacaacataa
     5941 atgacaccta cgaatacaaa tgcaatagca atcatcgtcg aaataaagac gatacccatt
     6001 aatggattaa ataataaaat gacaccgaaa acaataccta ataaattaaa aatcactgaa
     6061 attagtttta agctaccact acccgtaaac gtaaataatc ctgcaataga actaaaaatg
     6121 aaccagaatg caaacatgta gataaaaaat gccgagctag cgccaacatt aaaaatgaca
     6181 ataagaccaa agagaatatc tacaatcccc ataaacagaa tccaattttg gttaccacct
     6241 actaaagctt ttgcttttct acggtaaatg atttgaatca caccgttaat taatacaaac
     6301 agtccaatca accaggtaat agcataaaaa ttttcaattg gaaatgtaaa aataacgact
     6361 gccaacatta ataataatac acccattatt aaactagacc atttaatacc ttgatttcct
     6421 ttttccataa caatcaattc ctttcaaata aagccctctt ttaattttta caaaaaattt
     6481 attatacaat aattaaattc ccttttctca atttacacaa acatcatcac ttagaaagca
     6541 ccattgcttt tgtctaatat gtacgattaa tctgtatcct cactcaaaaa tctactaagt
     6601 agcgatagct ttcaactctg taaggttcat tcattgaatt gtaagtttag ataaaggcta
     6661 ctactccaaa cattatttaa aatgaaatta aatacaaaag gagtgagact atgacaatat
     6721 tatcagtgca acatgtttca aaaacatacg gtaaaaagca cacatttcaa gcacttaaag
     6781 atattaactt tgacatacaa aaaggcgaat tcgttgcgat tatggggcct tctggatcag
     6841 gtaagacaac cttattaaat gtactaagtt caattgacca aatttctagc ggtagcgtga
     6901 ttgctaacgg acaagagctt aataaactta atcaaaaagc acttgccaaa ttccgtaaag
     6961 aatcattagg tttcatcttc caagattaca gtattctgcc gacattaact gtcaaagaaa
     7021 acattatgtt acctttatct gttcaaaaaa tgtcgaaagc aacaatggaa gaaaattata
     7081 aagcgattac tacagcatta ggtatttatg acctaggaaa taaataccct agcgaattat
     7141 ctggtggtca acaacaaaga actgcagcag cgagagcatt tgttcacaaa ccacaaatca
     7201 tatttgcaga tgagccaaca ggcgcactcg actcgaaaag tgcaaatgac ctattacaac
     7261 gtttggaaga aatgaataaa tcgtttgata caactattgt catggttaca catgatccgg
     7321 ttgcagcaag ttttgcagaa cgtgtcatca tgctaaaaga tggccaaatt catacacaac
     7381 tttatcagga aggacgttct aaacaggcct tttatgaaga cattgtacat cttcaatcag
     7441 tattaggtgg tgtctcaaat gacatttaac catatcgttt tcaaaaactt acgacaaaac
     7501 ttaaaacatt atgcaatgta tttattttca ttatttttta gcatcgtctt atatttcagt
     7561 tttacaacct tacagtttac taaaggtgta aataatgacg actcgatggc catcattaag
     7621 aaaggtgctt tagtcggatc aatattttta tttatcatta ttgtcatctt tttaatgtat
     7681 gccaatcatt tattcgtgaa acgccgtaca cgtgaatttg cgctatttca gttgattggt
     7741 ttaacacgac aaaacatttt gaaaatgtta gcacttgaac aaatgattgt atttttaatc
     7801 acaggtgttg tcggtgtatt atgtggcatt gcaggtgcac aattattgct ttccatagta
     7861 tctaaattga tgtcgttatc gattaactta tcgatacatt tcgaacctat ggcccttgtt
     7921 ttaactattt tcatgctaat tattgcgtat gtactgattt tatttcaaag tgctttattt
     7981 ctaaaaagac gtagtatctt atcaatgatg aaagattcaa ttaaaactga tgccacaact
     8041 gctaaagtaa cgactgcaga ggttatttca ggcgtattag gtattgctat gattgcacta
     8101 ggctattata tggcgacaga aatgtttgga acatttaaag cactaactat ggctatgaca
     8161 tcaccgttta tcattttatt tttaacggta gtaggcgcct atttattctt cagaagttcc
     8221 gtgtcactaa tttttaaaac tttgaaaaaa tctaaaaatg gacgcgtatc tattacagat
     8281 gttgtattta catcgtctat tatgtaccga atgaagaaaa atgcgatgtc tttaactatc
     8341 attgcaatta tttctgcagt tacagtaact gtattgtgtt tcgcggcatt atctaaatca
     8401 aatacagatc aaacccttac atctatggca ccaaatgaat ttaacgtggt cgctactcaa
     8461 gatgctaaac aatttgaaac taaactaagc caacaacaaa ttacattttc taaaaatgct
     8521 tacgaaacta ttactgtaga taatgttaaa gatcaggtta ttactttgga aaatggtagt
     8581 gattcaggtc gcacgaattc aatattaagt gcaaataaca aggtaacagg caacaatgcc
     8641 atcattacaa atacaaaatc acttcctaac attattaata tccatttaaa caaagattta
     8701 gtagtaaaag gaactaagaa tgaaactttc cgtgttacac aagaagacaa aggtagggtt
     8761 tatcctttaa atctaagctt caactcacct gtcgtcgaag taagtcctga aaagtatcag
     8821 cagttgaaaa cacaaaataa cgttcatact ttttatggat atgatattaa acaaacatca
     8881 caaaaggaaa aagctcaagc aattgcaaaa cagtttggag acaaagtcat cacttatgat
     8941 gaaatgaaaa aagaggtcga tgctactaac ggtattctaa tatttgttac atcattttta
     9001 ggattagcat ttttagtagc agctggatgt attatttata ttaaacaaat ggatgaaact
     9061 gaggatgaac taagtaactt cagaatatta aaacgcatag gctttacgca tacagatatg
     9121 cttaagggat tattattaaa aattactttc aacttcggtt taccattatt gattgctata
     9181 ttacatgcag tattcgccgc aatagcattt atgaaattga tgggtaatat ttccttcatg
     9241 ccggttattg tagtaattgt tgtatacacc ctcatttaca ttacttttgc tttaattgct
     9301 tttgtacatt caaacaaatt gattaagaaa accatttaac acttacgatt aaaagatgct
     9361 tcattgagat tttgatttta agcaatgcag tataactatt aactgattta taaaaaggag
     9421 agattgaatc atgaaattta gagaattagt aaaacaatca tacgaggatt tgaaaaattt
     9481 gactgtaact tggtataacg tgattgcttt agcaatgatt gttattgtct taagcgtagt
     9541 tacaacacca attataggta ttccagctgg tttattaggt ggcgcttatt atttaaaacg
     9601 tcgtgaagaa aaaggtaaat aagcacattc catcggcata taacattgct atgacctttc
     9661 aagtcagatt ttaatatcaa caagtagcat ttatcgctgc ttgtttttta tgaattgctt
     9721 ataaaatcgt catgtttaaa cgttatgtca ctttgaaaac tagcaaacac cataaaagac
     9781 tacaattttt tatttttata aaccccttta aatcaacgtt tctaaggttt ttaaaaatct
     9841 ttttaaattt attttcaaaa aacactgtac attatgccaa tatgagcgta tagttggtct
     9901 tacgtaataa aagctcgtga attaaattgt agtgtatttg tttagaatat cctctttttt
     9961 agttatgaat ttgttacaaa tattaagtgc aaaagcacat ggaggttttc tatatgaata
    10021 acggtacagt aaaatggttt aacgcagaaa aaggttttgg tttcatcgaa caagaaaatg
    10081 gcggagacgt attcgtacat ttctcaggta tcgctagcga tggctacaaa actttagaag
    10141 aaggtcaaaa agttactttc gaaatcactg aaggtcaacg tggagaccaa gcagttaacg
    10201 tacaaactgt ttaatcttac aaaataaaac gactcattat aaatgaatcg ctttgattac
    10261 caacaaggtt ctaaatcttg ttggtatttt tttatgctat tttggataat atgagacaat
    10321 acgatgatga tttataaaaa cttatttgcc atatacacac taaggaaaat tatgaggctg
    10381 agtataccaa ttgctgatat aatagaaagc gcatttgtca tcgtatttct agctttagtc
    10441 gtttgtactt cactaggatc ttttccattc ataaaagcaa caattcgatc gtagttgtcc
    10501 attttatgca ctttttttaa atcttctatt ttcatagatg cataaaaacc tattcccaaa
    10561 atgattgcca ccgttatacc ccaagtccaa ttccaataaa caactaaagg tcccattaaa
    10621 attgcagcta atgtcatgaa aataagcatc acatatgtcc ataagttaag acttcgttgc
    10681 actttaatat caggtacaaa tggaatagtc ccttttacta aatcatctaa agttacattg
    10741 aacaattcgc acatcattaa taagttatgt atgtctggta agcttttgtc attttcccaa
    10801 ttagaaatac tctgcctaga tacatataac ttttcagcaa gatattcttg tgaataacca
    10861 tctcgttccc tatacttttt aatttgttta ctcagattca attacatacg cctccagtca
    10921 attagtacaa tcattacaat cagactatca gataaactta gttaaagtca cctttatgat
    10981 tgactaaatg ttttgacata cgacaacatc ggggttcgaa gactccctta agacagacct
    11041 gtataattac gcgcttgtaa ttttctcaat gccaacttga atttcactta ttaatgatgc
    11101 aacttttcca tgctattcaa tgcttgcaaa gttagtccta gttaagagat tacgttcaca
    11161 ctatttacta ttcgattttc cagctttcca actatgcttt tctaatcttt tttgtgctat
    11221 aaattctacc gcttcaaaaa tacatactgc actgatgaac cagcttagga aaccaacaat
    11281 aatgctaatc aaccacatgt gcgtcatatg taacatcatc catgtaacta aaatacaaac
    11341 taatacccct gtcataccaa acactgcgcc acgacttaca tgcacagcga ctttatcacc
    11401 atattgcata cctgtaataa acatagacac taaaaatact gccggaaacg ttgcaaatat
    11461 accgccaaat tctttccaag gtagtgttac agatacaata taacttaata atactgcaaa
    11521 tccccccaca aaaaatttca ttaatgttaa tttcatgatg ttctgcctcc aaaaatttat
    11581 tacttaataa ctgaatacaa ctaattatca cgcattttcc aaactataaa tgtctaatac
    11641 tgaatattgc gagggaaata acaaaccaac aaacaactgt gaatattgcg cctttccgat
    11701 aacctttatg cttaatatag agagatgtca aaaatacagt taatatacaa gacagaatac
    11761 cgacaattgc tccagtacta agattcatcg acatctccac taattgcgta ccacgatgat
    11821 ctaaagctaa tgcgataata gccgcaagaa agactgccgg catagtagct ataatacctc
    11881 ctaacttacc acctacctta tcagcaataa ttgaagctaa tgcaacagcg ataccaccaa
    11941 tgacaaaatg aagaattgca cttccaattg aaaacatctt caacgtacct ttctataatt
    12001 aattcaatac aaaacgtatc attcacacaa agtgtaaaac ttaaattttc tactgtaaag
    12061 gcaactcaag cgatgttcat aaagctttca tgatactttc ataaactagc aataaagggt
    12121 tcaaatgaag gtatatccag ctcaaattag ggagtgggac agaaatgata ttttcgcaaa
    12181 atttatttcg tcgtcccacc cc
//
LOCUS       DAOBBH010000067        12013 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.067, whole genome shotgun sequence.
ACCESSION   DAOBBH010000067 DAOBBH010000000
VERSION     DAOBBH010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12013
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.067"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(36..398)
                     /locus_tag="P6L01_001346"
     CDS             complement(36..398)
                     /locus_tag="P6L01_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDP3030420.1"
                     /translation="MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVA
                     AITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEV
                     DNALMISLGLNAVAHQKN"
     gene            complement(395..565)
                     /gene="mazE"
                     /locus_tag="P6L01_001347"
     CDS             complement(395..565)
                     /gene="mazE"
                     /locus_tag="P6L01_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HDP3030421.1"
                     /translation="MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDC
                     NETYLSSNSTNE"
     gene            complement(650..1798)
                     /gene="alr"
                     /locus_tag="P6L01_001348"
     CDS             complement(650..1798)
                     /gene="alr"
                     /locus_tag="P6L01_001348"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDP3030422.1"
                     /translation="MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYG
                     LGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVA
                     LTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTNTYQEVIEIIQQYEQL
                     VFEGVFTHFACADEPGDMTTEQYQRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAI
                     RPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTT
                     IALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDN
                     HRESPQSVEVVAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK"
     gene            complement(1864..2223)
                     /locus_tag="P6L01_001349"
     CDS             complement(1864..2223)
                     /locus_tag="P6L01_001349"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDP3030423.1"
                     /translation="MIHGIGVDLIEIDRIKVLYSKQPKLVERILTKNEQHKFNNFTHE
                     QRKIEFLAGRFATKEAFSKALGTGLGKHVAFNDIDCYNDELGKPKIDYEGFIVHVSIS
                     HTEHYAMSQVVLEKSAF"
     gene            complement(2227..2718)
                     /locus_tag="P6L01_001350"
     CDS             complement(2227..2718)
                     /locus_tag="P6L01_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDP3030424.1"
                     /translation="MRDYNFMSPHAKKVMRLSAVLFWIPIALVLLIAFNILNWLFWEI
                     LDNNISIVSSVIIILLVAVFTLVIAPVYRYKHCRYAFEKHHLRVRNGILFLDEKVIPY
                     FRIQNIDIDVGPIMRRYRLATLTLYTAGGQAEIELIDRSEARRLKRWLNNERHLENNK
                     NEE"
     gene            complement(2705..4288)
                     /locus_tag="P6L01_001351"
     CDS             complement(2705..4288)
                     /locus_tag="P6L01_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDP3030425.1"
                     /translation="MSEPQKLHPISYFNGIINAIKQNIVVFFIFIIFQLKDFDYTNPE
                     SYLWIGIVFVFFLISYISQIVGIMNTRYWIEDNYFILTTGIFNKKRKELNIKRIQSVD
                     MTQGVVNQIIGGVDLQIKTPSDGIVLSVISKKQGEYLERYIDQLQTELKTEVAQKSSA
                     EKNDNCNHDIEGNVEDIAEGRLDEVQNTDSMLNQKNKRIPIYQMNFKESLFMAMTSGA
                     IGVTLAALVPIYGAVRELIPWSKLNHEISQWVQIVSISVLIIILLVLIVAYIIGTIIT
                     MIRFYGFSVMLENEQLKIEYGLFNKKKVTVPTKRLQAVVERQSYIRRLFGYTAIHFII
                     TSDFENKKIEDDDNDSGSVPILPFIKRDKAYQIIQNLVPDLSYQQVNEGMPLSGFHRH
                     FLIPSIIVIAMAGIGCYFWNQLLLLFTIITIVIIGLFVIKGYLYVKHAGFKMTEEQIT
                     IQNFELLKRNIYYFKAHHIIGFDTWQHTLLKRNNLNNFSFIVAKGVGAQSINLKYARS
                     EDANKLKSWYLRGENDEGL"
     gene            complement(4281..4760)
                     /locus_tag="P6L01_001352"
     CDS             complement(4281..4760)
                     /locus_tag="P6L01_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030426.1"
                     /translation="MSDYTKLTKSPKSALLYYYITNGLEFILSVAVYVIFYFIWLRFE
                     WPHYLLYILLVLGALSTLKLFIKPLWQYHCRFYKVDQLSVQYRTSFFIYKEETSRIER
                     LQYLSIKSNPISKVLNLYKVGFMTAGHTIYLPMMSHDDVKIIEARTMSNLRGVESDV"
     gene            complement(4968..5528)
                     /locus_tag="P6L01_001353"
     CDS             complement(4968..5528)
                     /locus_tag="P6L01_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDP3030427.1"
                     /translation="MNTIRNSICLTIITMVLCGFLFPLAITLIGQIFFYQQANGSLIT
                     YDNRIVGSKLIGQHWTETRYFHGRPSAVDYNMNPEKLYKNGVSSGGSNESNGNTELIA
                     RMKHHVKFGNSNVTIDAATSSGSGLDPHITVENALKQAPRIADARHVSTSRVADLIQH
                     RKQRGVLTNDYVNVLELNIALDKMKD"
     gene            complement(5548..7575)
                     /gene="kdpB"
                     /locus_tag="P6L01_001354"
     CDS             complement(5548..7575)
                     /gene="kdpB"
                     /locus_tag="P6L01_001354"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDP3030428.1"
                     /translation="MHHVNKYFNQTMVIEALKMSFYKLNPKQLIKNPIMFVVEVGMVL
                     TLILICFPDIFGTSYLSRGYLITIFIILLITILFANFSEAFAEGRGKAQADSLRQAQS
                     NLTARLIEENGAYRIVNATELKAGQNIRVENGETIPADGVVINGLATVDESAITGESA
                     PVIKESGGDFDGVIGGTLVTSDWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIAL
                     FTLLTTLTIIFLVVIVTLYPIASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGM
                     DRVTQFNVLAKSGRAVEVCGDVDVMILDKTGTITYGNRIASEFLPVNQQMLEKLIVAA
                     YMSSIYDDTPEGKSIVRLAKQMYINELPKDIDGTYKPFTAETRMSGIITNEISVFKGA
                     PNSMINLVKQQQGNIPLNIESLCMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVE
                     RFTELRKMGIETVMCTGDNALTAATIAKEAGVDRFVAECKPEDKIKVIKDEQAKGHIV
                     AMTGDGTNDAPALAQANIGLAMNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMT
                     RGALTTFSLANDVAKYFAILPALMMSTIPEMTSLNIMHLSSPKSAIISALIFNALIIV
                     ALIPIAMKGVKVKGYSIDRIFINNMLIYGLGGLIVPFLGIKLIDMIVQFFV"
     gene            complement(7594..9270)
                     /gene="kdpA"
                     /locus_tag="P6L01_001355"
     CDS             complement(7594..9270)
                     /gene="kdpA"
                     /locus_tag="P6L01_001355"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HDP3030429.1"
                     /translation="MEIILFLTMMVMITYVFSGYLYRVALVQSSRVDLIFTRFENMCF
                     KIIGTDLEHMSAKTYVKHFLAFNGFMGFITFVLLIVQQWLFLNPNHILNQSIDLAFNT
                     AISFLTNSNLQHYNGESDVTYLTQMIVMTYLMFTSSASGYAVCIAMLRRLTGLTNIIG
                     NFYQDIVRFIVRVLLPLSCLISILLMTQGVPQTLHANLMIRTLSGHIQHIAFGPIASL
                     ESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKR
                     VHRHALILFVAMFFIFIAILTLTMWSEYRGNPILANLGIYGPNMEGKEVRFGAGLSAL
                     FTVITTAFTTGSVNNMHDSLTPIGGLGPMVLMMLNVVFGGEGVGLMNLLIFVLLTVFI
                     CSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILILVFSALAFMIPGASESITNPSFH
                     GISQVMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMIASSLVN
                     KKSYHQDKYTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK"
     gene            complement(9294..9404)
                     /gene="kdpF"
                     /locus_tag="P6L01_001356"
     CDS             complement(9294..9404)
                     /gene="kdpF"
                     /locus_tag="P6L01_001356"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDP3030430.1"
                     /translation="MLLCCKEVMAMLIVLLLVVIALVLYLFYALIRSEKF"
     gene            9542..>12013
                     /locus_tag="P6L01_001357"
     CDS             9542..>12013
                     /locus_tag="P6L01_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HDP3030431.1"
                     /translation="MSNTESLNIGKKRGSLTIYIGYSPGVGKTFEMLSNAIELFQSNV
                     DIKIGYIEPHQRDETNALAEQLPKITTNFTKHGSHHFQYLDVDRIIEESPTIVLIDEL
                     AHTNISRDRHEKRYMDIEEILNHGIDVHTTLNIQHIESLSSQIELMTGVHVKERVPDY
                     FIMSADVLEVVDISPEQLIKRLKAGKVYKKDRLDVAFSNFFTYAHLSELRTLTLRTVA
                     DLMSDKEKVRHNHKTSLKPHIAVAISGSIYNEAVIKEAFHIAQKEHAKFTAIYIDVFE
                     KNRQYKDSQKQVHQHLMLAKSLGAKVKVVYSQTVALGLDEWCKNQDVTKLIIGQHIRN
                     KWRDFFNTPLIDHLMSFEHSYKIEIVPIKQIPVELKMNKSPYRPKGKRFAIDMLKMIL
                     IQIICVMMGLWIYQLDKHESSTIILMIFLIGIILLSIWTRSFIIGFLAAIINVFVFNY
                     FFTEPRYTFEVYRFDYPITFIVSILTSILTSALLKQIKFQYSITKKQLYRTDLLFQFN
                     DSIKQTYTVENLLINAGYQINQLLQQSITIYVINQSKVIKTIPLQNHIDNTTQQHEQA
                     LSWVIKNERQAGATTDTFPGINKWLIPIGTSPIKGILAIDYQSSQVINPYDASILESM
                     LNELSLAVENVTLLKQTRESMLQAERQLTHSNFLRSISHDIRTPLTTIMGNLDILVSH
                     SKDMSIIEKEQLLVHSFQESQYLYLLVTNILSLTKLQSSNVQIKLQPYLVSELVEEID
                     MILERRHLKKRITVSSSVNLQFIHIDSKLILQALFNLIENAVKHTSTDTKINLSIRYA
                     SYEQIEFAVIDEGPGISLEEQQKIFE"
BASE COUNT         4032 a         2253 c         1705 g         4023 t
ORIGIN      
        1 tttatttatc tctgaaaaat acatataata gacgcctaat ttttctggtg agctactgca
       61 ttcagcccta aactaatcat tagtgcatta tctacttctt tcattttatc atcggataag
      121 tacgtcagtt tttctttcaa tcgtttttta tcaagtgtac gaatttgttc taataatata
      181 actgagtctt tatccaattt atactttttc ttttcaatct ctacatgtgt cggtattttc
      241 gctttattaa tcctaccagt tattgccgca acaataactg taggactata tttattacca
      301 gtatcatttt gaattatgac tacaggtctg actccccctt gttcagatcc ctgtactggt
      361 gataaatctg ctaaataaac atctcctcgt ctaatcattc attcgttgaa ttagaagata
      421 aatatgtttc gttgcaatcg catgcttcac actctatcgg aaaagcttcg ttcgctaggg
      481 agagatttaa atcagccatt tgtgaatatc cttcttttaa agattgttct aagctatgac
      541 ttctattttg actaaaagat aacatatgag aacctccaat tgattccaaa agttattaaa
      601 tgacctttaa ttactctaat gataacaaat tttatatgca ctgaccatat tattttaaca
      661 attcatttgt tacaaaacgt tgatcaccat catgatagat tcgcggcaaa cgtctcgaca
      721 agttacaaag cacttcataa ttaatagtat gttgcttttc agctaccacc tctaccgact
      781 gtggactttc tctatgatta tctattaaaa tcaccgaatc tccagcttta acttgatctg
      841 gcactttaac aattgtctga tccatacata cgcgaccaat aacttcgcat tgatgaccat
      901 ttacatttac aaagctacct tgcattatgc gtaaatagcc atctgcatat ccaataggta
      961 acaaagctat tgtagttggg tcagtagctg tataagttgc accataactt acagactcac
     1021 ccgcttgtag cgtctttgtt tgaactacat tagcaattaa ttgcacactt ggtttaaggt
     1081 gtactttaac tttttgctgt acatactctg atggataata tccataaagg gaaattcctg
     1141 gtcttattgc attacagaat tggcaatcca ttaatagaga gcctgctgag ttctgacaat
     1201 gtatatattc aggtttaatt gcttcattga ccatatcttt aaaacgttga tattgttcag
     1261 ttgtcatatc tcctggttcg tcagcacagg caaagtgtgt aaacacgcct tcaaatacaa
     1321 gttgctcata ttgttgaatg atttcaatca cttcttgata cgtattagta tctttaatac
     1381 ctaaacgtcc cattcctgta tctaatttaa tgtgcaacca taactttttc tcttgctcac
     1441 cagaaatgtt tttaattgct tctttcaacc actgtttaga cggaaccgtt aaggcaactc
     1501 tgtgttgtat cgctttatca atatctttag ctggtaacac acctaagact aaaattttag
     1561 cagtaatccc atgcattcta agttctatcg cttcatctaa cgttgctaca gcaaaaaatg
     1621 tggcgccatt ttccattaaa tgacgtgcta ctttaacact acctagtcca taggcattgg
     1681 ctttaacgac agccatcact gttttatttg gatgcaatgt actgaatact ttgaaatttg
     1741 atgcaacagc gtttaaatct acattcatat acgcagatct ataatattta tccgacatat
     1801 tacttcctcc tgtaattccc acacgtttta aaactagatc ttaattatca ttgtataaca
     1861 aatttaaaat gctgactttt ctaaaacaac ttggctcatc gcataatgct cagtgtgtga
     1921 gatactaaca tgtacgatga acccttcgta atcaatcttt ggtttgccaa gttcgtcgtt
     1981 gtaacagtct atatcgttaa aagctacatg ttttcctaag cctgtgccta atgctttact
     2041 gaacgcttct tttgtagcaa acctgccagc taaaaattca attttacgtt gctcatgtgt
     2101 gaaattgttg aatttgtgct gttcattttt agttaaaatc cgctcaacca attttggctg
     2161 cttactatat aacactttta ttcgatcgat ttcgattaaa tctacaccaa ttccatgtat
     2221 cattgattat tcctcatttt tattgttttc taaatgtctc tcgttattta accatcgttt
     2281 caatctacgt gcttcacttc tatctattaa ttcaatttca gcttgaccac ccgctgtata
     2341 aagggtcaaa gttgctaatc gatagcgtct cattatagga ccaacatcga tatcaatatt
     2401 ttgaatacga aaatatggta ttactttttc atccaaaaat aaaatgccgt ttcgtacacg
     2461 caaatggtgt ttttcaaatg catatctgca gtgcttatat cgatatactg gcgctatgac
     2521 aagcgtgaaa acagcaacaa gtaatattat aatcacacta ctaacaatgg atatattgtt
     2581 atctaatatc tcccaaaata gccagttcaa aatattaaat gcgattaaaa gtacaagcgc
     2641 tatgggtatc caaaacagca cagcacttaa cctcatcact tttttagcgt gtggtgacat
     2701 aaaattataa tccctcatca ttttcacctc ttaaatacca tgatttcaat ttatttgcat
     2761 cttcacttct tgcgtatttt aggttaatcg attgggctcc cacaccttta gcaacaataa
     2821 agctaaaatt atttaaattg ttgcgtttaa gtaatgtatg ttgccaagtg tcaaatccta
     2881 taatgtgatg cgctttaaaa taataaatat ttcgtttcaa tagctcgaaa ttctggatag
     2941 taatttgttc ttctgtcatt ttaaaacccg catgtttgac ataaagatat cctttgatca
     3001 caaataaacc aataatgact attgttataa tcgtaaataa caataataat tgattccaaa
     3061 aataacagcc aatacctgcc atagctatga caataatact aggtattaaa aagtgtctgt
     3121 ggaaacctga caaaggcata ccttcattaa cttgttgata agataaatct ggtactaaat
     3181 tctggataat ttgatatgct ttgtctcgtt taataaacgg caatatcggc acactacctg
     3241 aatcattgtc atcatcttcg atttttttat tttcaaaatc acttgtgatg ataaaatgta
     3301 ttgctgtgta gccaaataat cttcgtatat atgactgacg ttcaacaaca gcttgcaatc
     3361 gtttcgttgg tacagttact ttcttcttgt taaagagacc atattcaatt ttaagttgct
     3421 cattttcaag catcaccgaa aagccataaa atcttatcat tgttataatc gttccaataa
     3481 tatatgccac tattaatact agtaaaatga tgattaatac tgaaatactt acaatttgaa
     3541 cccattgact aatttcatga tttagcttcg accatgggat caactctctt acagccccgt
     3601 aaatcggtac taaagctgct aacgttacac caatggcgcc actggtcatt gccataaata
     3661 gtgattcttt aaaattcatc tgatatatag gaatgcgttt atttttctga ttaagcatac
     3721 tatcagtgtt ctgcacttca tctaagcgac cttctgcgat gtcttccaca ttaccttcaa
     3781 tgtcatgatt acagttgtca ttcttctcag cactagactt ttgcgccact tctgtcttca
     3841 actctgtttg caattgatca atatatcgtt caagatattc accttgtttt ttcgaaataa
     3901 cacttaagac aataccatca cttggtgttt tgatttgtaa atccacacca ccaataattt
     3961 gattgacgac cccttgtgtc atatcaacgg actgaatacg ttttatattt aattctttac
     4021 gctttttatt aaaaatacct gttgttaaaa tgaaataatt atcctcaatc caatatcgcg
     4081 tgttcataat tccgacaatt tgagaaatgt atgatattaa aaagaataca aatacaatac
     4141 ctatccataa atatgattcg ggattcgtat aatcaaaatc tttcaattga aagataatga
     4201 aaataaaaaa gacgactatg ttttgtttga tagcattgat tatgccatta aaatatgaaa
     4261 tcggatgtaa tttttgaggt tcagacatca ctttcaaccc ctctcaaatt cgacatagtt
     4321 ctcgcttcga ttattttaac atcgtcatga gacatcatcg gtaaataaat agtatgacct
     4381 gcagtcataa atccaacttt atacaaatta agcactttac taattggatt agatttaatc
     4441 gacaagtatt gtaaacgttc aattcgactc gtttcttctt tatatataaa aaatgatgta
     4501 cgatattgta cacttagttg atcaacttta taaaagcgac aatgatattg ccataaaggc
     4561 ttaataaata attttaatgt actcagagca cctaaaacca acaaaatata aagtaagtaa
     4621 tgtggccatt caaatcttaa ccatataaaa taaaaaatga catacacagc tacactcaat
     4681 ataaattcta agccattcgt aatgtagtaa tacaacaatg ctgacttagg actcttagtc
     4741 aacttagtat aatctgacat aacccctctc cccaaataaa aaattatacg gatttataat
     4801 ctatttcatt ttatttttat atgatgataa ttatagcata tggaatattt catgctaatt
     4861 tattcttcct aaaggtacat ctaaaaattt aattaagcag aaagtgcttg aattgctaaa
     4921 aagacaccat gttataattt tatcaacatg atgcctttca tctataatca atctttcatc
     4981 ttatcaagag cgatatttag ttcaagcaca ttcacataat catttgttaa cacaccacgc
     5041 tgcttacgat gttgaatcaa gtcggccact cttgaagtag atacatgacg agcatcagca
     5101 atacgaggtg cttgcttcaa tgcattttcg accgtaatat gcggatctaa gcccgaccca
     5161 gaacttgttg cagcatctat tgttacattt gaattcccaa atttaacatg atgtttcatg
     5221 cgtgctatta attcggtgtt tccattcgat tcattacttc cacctgaaga tacgccgttt
     5281 ttatataatt tttcaggatt catattataa tcaactgcac tcggtctccc gtgaaaatat
     5341 cgtgtctctg tccagtgctg tccaatcaat tttgatccaa ctatacgatt gtcatacgta
     5401 attaaactgc catttgcttg ttgataaaaa aatatttgac caattaacgt gatagctaac
     5461 gggaataaaa atccacataa taccatagtt attatcgtta aacaaatact atttcttatc
     5521 gtattcatgg tacaggctcc ttcctcttta cacaaaaaat tgtacaatca tatctattaa
     5581 tttaatgcct aaaaacggga cgattaatcc acctaatcca taaatcaaca tattatttat
     5641 aaagattcta tcaatgctgt aaccctttac ttttacacct ttcatggcaa ttggaattaa
     5701 ggcaacaatg attaatgcat tgaatatcaa agctgatata attgcagatt taggtgacga
     5761 taaatgcata atatttagtg aagtcatttc tggaattgtt gacatcatta gtgcaggtaa
     5821 aattgcaaag tattttgcta cgtcattagc caaactaaat gtcgttaatg cacctctcgt
     5881 cattaataat tgtttgccta tttttacaac ctctattaac tttgtaggat tcgaatctaa
     5941 atcaattaga ttagctgcct ctttagcact aattgtccct gagttcatag ctaatcctat
     6001 attcgcttgt gctagcgcag gtgcatcatt tgtaccatct cctgtcatcg caacaatatg
     6061 gcctttcgct tgttcatctt tgatgacttt aattttatct tcgggtttac actctgcaac
     6121 aaatctatca accccggctt cttttgcaat tgtagctgct gttaaagcat tatcacctgt
     6181 acacataact gtttcaatcc ccatttttct caattcagta aatcgttcta caagaccatc
     6241 tttaatcaca tcttttaaat aaatcacgcc aagcatgaca ttgttttcaa tgactattaa
     6301 tggtgtgcca cctttactcg atacatccat acagagagac tcaatgttaa gaggaatatt
     6361 gccttgttgt tgtttgacaa gatttatcat actattaggt gcacctttga ataccgatat
     6421 ttcatttgta atgattccgc tcattctagt ttcagctgta aaaggcttat atgtgccatc
     6481 aatgtcttta ggcagctcat ttatatacat ctgcttcgct aatcgtacaa tactttttcc
     6541 ttctggcgta tcatcgtaga ttgatgacat ataagcagcg actatcaatt tttccagcat
     6601 ttgttgattc actggtaaaa attcactagc gattcgattg ccataagtga ttgtccctgt
     6661 cttgtctaaa atcattacat cgacatctcc acatacttct acagcacgcc cacttttcgc
     6721 taatacattg aattgagtaa cacgatccat gcctgcaata ccaatcgccg ataacaaacc
     6781 accgattgtc gttggtatta aacatactgt taacgcaatg agcatcgcaa taggtaaaat
     6841 taaatgcagg taagatgcta ttggatataa cgttacaata acgactaaaa atataattgt
     6901 taacgttgtt aataatgtaa aaagtgcaat ttcatttggt gttttatttc tttccgcccc
     6961 ttcaactaag gcaatcattt tatctaaaaa agatgtaccc gcttcactct caacacgtat
     7021 ttctaaccaa tcagatgtta caagtgtacc gccaatgact ccatcaaaat cgccacctga
     7081 ttcttttatc acaggtgcag actcaccagt aattgcagat tcatcaacgg ttgctaatcc
     7141 atttattaca acgccatcag cagggattgt ttctccattt tctacccgaa tattttgtcc
     7201 ggcttttaac tctgtggcgt tcactatccg atacgcacca ttttcttcta tcaatcgagc
     7261 agttaaattt gattgtgctt gtcttaaact atcagcttgc gcttttccac gaccttcagc
     7321 aaaggcttct gaaaaattag caaacaatat agttattaat aatatgataa aaattgtaat
     7381 caaataacct cgcgatagat agctagttcc aaatatgtca ggaaaacata ttaatatcaa
     7441 cgttaaaacc attccaacct caacgacaaa cattatcgga ttttttatta attgtttagg
     7501 attcagctta taaaaactca ttttcaaagc ttctatcacc attgtttgat tgaaatattt
     7561 atttacatga tgcatttttt ccatcccttt actttatttt aaagttaaaa attcaccaat
     7621 aggaccaagt aatagtactg gaataaatgt caaaccactt agtaaaacga taaatacgat
     7681 tagtgatacg ccaaaataag gtttatcaat cgctattgta tatttatctt gatggtatga
     7741 ttttttattc actaaacttg atgcaatcat taattgcaaa ataattggta tataacgaga
     7801 aagcaacata atgattcctg tagagatatt ccagaatgtt gtatcatctt tcagtccttc
     7861 aaaccctgat ccattgttcg cagcagctga tgtcatttca tacataactt gtgaaatacc
     7921 atgaaaagac ggattcgtta tactttcact tgctccagga atcataaaag caagtgctga
     7981 aaatactaaa attaaaattg ggtgtatgag aaagactaag acaatacatt tcatttcacg
     8041 ggcgccaatt ggcatattta aatattctgg tgttttacca accatcaaac tgcatataaa
     8101 caccgtcagt aagacaaata tcaataaatt catgagtcct acgccttcgc caccaaatac
     8161 aacatttagc atcattaata ccattggtcc taatccacct ataggcgtta agctatcatg
     8221 catgttatta acagaacccg ttgtaaatgc cgtcgtaata actgtaaata gtgctgacaa
     8281 acctgctcca aaccgtacct ctttaccttc catattcggt ccataaatgc ctaaattcgc
     8341 tagtattgga ttaccacgat actcactcca catagttaat gtaagaattg ctataaaaat
     8401 gaaaaacatt gcgacaaata atatcaacgc atgacgatgt actcgtttac catgtctact
     8461 taacatgcga ccaaataaga acaacattga cataggaagt aacatcatac tgcccatttc
     8521 tataaaattg ctccaaatat ttggattttc aaaaggtgtt gcagaatttc ctgctaaaaa
     8581 tcctccacca ttcgtaccaa gatgttttat tgattcaagt gatgcaatag gtccaaatgc
     8641 aatatgttga atatgtccgc ttaaagtccg aatcattaaa ttagcatgca acgtttgtgg
     8701 tacaccttga gtcatcaata aaatactaat taaacatgat aatggtaaaa gtactcggac
     8761 aataaaccga acaatatctt gataaaaatt accaatgata ttagttaatc cagttaaacg
     8821 tctcaacatc gctatacaaa cggcgtaacc tgatgcacta gatgtaaaca ttaaatatgt
     8881 cattacaatc atttgcgtta aatatgtcac atctgattca ccgttatagt gttgtaaatt
     8941 actatttgtt aaaaaagata ttgctgtatt aaacgctaaa tctatcgatt ggtttaaaat
     9001 atgatttgga tttaaaaaaa gccattgctg aactattagc aatacaaatg ttataaaccc
     9061 cataaatcca ttaaatgcca gaaaatgttt gacatatgtt ttagctgaca tgtgttctaa
     9121 atctgtgccg ataattttaa aacacatatt ttcaaatcta gtaaatatta aatctactct
     9181 tgacgattgc accaatgcta cgcgatatag atatccacta aaaacatacg taatcataac
     9241 catcattgtt agaaacaaaa ttatttccat gataaccctc acttaatata tttctaaaat
     9301 ttttcactac gaattaaggc ataaaataaa tacaaaacta atgcaataac taccagtaat
     9361 aaaacgatga gcattgccat aacctcctta caacacaaca acatcgtaac aacttgttta
     9421 tgagagaaat attaattttc aaacttagtt attaagaaat cattaagatg tgtatgcaga
     9481 aataaatttt atagcattta attgtgaaga atattatgat attgctatcg aggtgaaggt
     9541 tatgtcaaac actgaatcgc taaacatagg aaaaaagcgt ggatctttaa caatttacat
     9601 cggttacagt ccaggtgttg gtaaaacatt tgagatgctt tcaaatgcca ttgaactatt
     9661 tcaaagtaac gttgatatta aaataggata tattgaaccg catcagcgtg atgaaacaaa
     9721 tgcattagct gaacaattac ctaaaatcac aaccaatttt actaaacatg gtagtcatca
     9781 ttttcaatat ttagatgtcg accgcataat cgaagaatcg ccgacaatag tacttatcga
     9841 tgagttagca catacgaata tttctagaga tcgtcatgag aaacgatata tggatattga
     9901 agaaatttta aatcatggta tcgatgttca taccactttg aacattcaac atattgaaag
     9961 tttaagtagt caaattgaac tgatgaccgg tgtacatgtt aaagaacgtg tacccgacta
    10021 tttcataatg agcgccgatg tattagaagt cgtagatatc tcacctgaac aattaattaa
    10081 acgcttaaaa gctggcaagg tatataaaaa ggataggcta gatgtagcat ttagtaattt
    10141 ctttacgtat gcccacctaa gcgaactgcg tacattgacg ttaagaacag ttgccgactt
    10201 gatgagtgat aaagaaaaag tccgacacaa ccataaaacg tcactcaaac ctcatattgc
    10261 tgtggcaatt agtgggagca tttataatga agcagtaatt aaagaggcat tccatattgc
    10321 tcaaaaagaa catgcgaagt tcactgctat ttatatagat gtattcgaaa aaaacaggca
    10381 atataaagat agtcaaaagc aagtgcatca acatctcatg cttgcaaaat cattaggagc
    10441 aaaagtaaaa gtagtttata gccaaaccgt tgcattagga ttagacgaat ggtgtaaaaa
    10501 tcaagatgta accaaattaa ttatcggaca acatattaga aataagtggc gagacttttt
    10561 caatacacct ttaattgacc atttaatgtc ctttgaacat agctataaaa tcgaaatcgt
    10621 tccaatcaaa caaatacctg ttgaattgaa aatgaacaaa tcaccctatc gtcctaaagg
    10681 caaacgtttc gccatagata tgttaaaaat gattttgatt caaataattt gtgtaatgat
    10741 gggactgtgg atttatcaac ttgataagca tgagtctagt acgattattt taatgatttt
    10801 tctcatcggc atcattttat tatccatttg gacgcggtcc ttcatcattg gctttttagc
    10861 agcaattatt aacgtatttg tttttaatta tttttttacg gaacctagat atacatttga
    10921 agtatatcgc tttgactatc ctattacatt tatcgttagc attttaacaa gtattttaac
    10981 gagtgcttta ttaaaacaaa ttaaattcca atactccatt actaaaaagc aactttatcg
    11041 tacagattta ttatttcaat ttaatgattc gattaaacaa acttatacag ttgaaaactt
    11101 actaataaat gctggatacc aaattaatca attattgcaa caatcaatta ctatatacgt
    11161 tatcaatcaa tcgaaagtaa ttaaaacaat accattgcaa aaccatatcg ataatacgac
    11221 tcaacagcat gaacaagcat taagctgggt aattaaaaat gaaagacaag ctggtgcaac
    11281 aacagataca tttccaggca ttaataaatg gttgatacca attggcacat ctccgattaa
    11341 aggcattcta gccattgatt accaaagttc tcaagtgatt aatccatatg atgcatcaat
    11401 attagaatca atgttaaatg aactttctct cgccgttgaa aatgtgacgt tacttaaaca
    11461 aacgagagaa tccatgttac aggctgaacg tcaactgaca cattcaaatt ttttaagatc
    11521 aatttcgcat gacatacgta caccgttaac aacaattatg ggaaatttag atattttagt
    11581 atcacatagt aaagatatgt ctatcataga aaaggaacaa ttacttgtac atagttttca
    11641 agaaagtcag tatttatatc tattagtgac aaatatttta tcattaacaa aattacagtc
    11701 atcaaatgtt cagataaaat tgcaacctta tctcgttagt gaattagtag aagaaatcga
    11761 tatgatttta gaacgtcgcc atttaaaaaa gcgtataact gtatcatcct ccgtaaattt
    11821 acagtttata cacattgaca gtaagttgat tttacaagct ttattcaatt tgattgaaaa
    11881 tgcagttaaa cacacttcta ctgacaccaa aatcaattta tctattcgtt atgctagcta
    11941 tgaacaaatc gaatttgccg ttatagacga aggacctgga attagtttag aagaacaaca
    12001 aaagatattc gag
//
LOCUS       DAOBBH010000068        11937 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.068, whole genome shotgun sequence.
ACCESSION   DAOBBH010000068 DAOBBH010000000
VERSION     DAOBBH010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11937
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.068"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            208..2163
                     /locus_tag="P6L01_001358"
     CDS             208..2163
                     /locus_tag="P6L01_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDP3030432.1"
                     /translation="MTDNYSLHIYKSMKMSSVKEANLKIIYWLQGTGVVSINLQQYDV
                     KRNDVTVIMLNDLYQIDGHDDSVCCVVEVSAKTFLKFMNANYMMRGKILTNEVASTFR
                     ILIKYLIYSKIKQQSYNANDKIINYMCVELMSLNTYDGSRHLVAEEVHDYLTNNHHKK
                     INRKDVINQVSITNKALSDMFKATPYSNFIQYLNHIRLEHCLIDILTSKKPIEEIASS
                     HGFNHYSRFIHLFKETYGNTPKLIRKRYSPTSHSINHSQLVEIDKNIIELFDNTNQSS
                     SSMREIDIPFEPTKRSQMYQPKNIYIKGSNFYWMDYYTIKQIIQRLGVNLENLHIIVT
                     IDTSNELSIEQITLLLQKIVNYNVNVVFRIKHEYKELLTSTERGKIEKLVATIFNMTF
                     ESNRCKIAFLINDLNTTAIQKLKRLIDNYIGVFELIYHLEPKELNTHYDKEIDHLIDY
                     FILPLRQIEQVSIACSKIICDVDLINENNDLKEKITMSDFNKVTELLTNYTKFRGLIM
                     HMSIISEASKEINLTRLSIFIFIINILNQLRGIVIYQNDTMIVTKFKHEVQCVICLPA
                     ALLNENSDAIRLSCKGLQQFSHAKTKQIVLSIDKDNHTSENGVLVNDIMQQHSYWKAN
                     FIERNIYDHSLMVSSNSIVHLKYHLEI"
     gene            2189..2932
                     /locus_tag="P6L01_001359"
     CDS             2189..2932
                     /locus_tag="P6L01_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HDP3030433.1"
                     /translation="MSKFKVKRLTAHILMLKVINDMLKYSFHLTLTQVKLLNLLVKYD
                     NSDSNSASIDRLLKMKEIQSKMALLQMLSYLNNHQWLLKGRDQRDQRKLTISINKMHI
                     DKIEYMNNELSDYIERYFGQYTFDFSCDYVSYLLSSQELFRNLKCYLNMCQLTLEELY
                     VLGILNLHKGQLTVKELQGEFHHPIFAVSPILKCLILKGLVKKERCELDERRVIVTIK
                     REKFSKVTMLIQACYNYLEKGIQVKLNNK"
     gene            2996..3322
                     /locus_tag="P6L01_001360"
     CDS             2996..3322
                     /locus_tag="P6L01_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDP3030434.1"
                     /translation="MILDNVNPNDLFPTEKKGPSVLGIIEYQVQGENEFEGAFIATNE
                     RLIMNVDMNGQFYYRSISYNEIEKIDYDGQTIMFKFNIGNVPMHDIKSDNVEMFVEYV
                     KQHMIV"
     gene            3750..4097
                     /gene="sarR"
                     /locus_tag="P6L01_001361"
     CDS             3750..4097
                     /gene="sarR"
                     /locus_tag="P6L01_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarR"
                     /protein_id="HDP3030435.1"
                     /translation="MSKINDINDLVNATFQVKKFFRDTKKKFNLNYEEIYILNHILRS
                     ESNEISSKEIAKCSEFKPYYLTKALQKLKDLKLLSKKRSLQDERTVIVYVTDTQKANI
                     QKLISELEEYIKN"
     gene            complement(4286..5122)
                     /locus_tag="P6L01_001362"
     CDS             complement(4286..5122)
                     /locus_tag="P6L01_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HDP3030436.1"
                     /translation="MDEQQWTGQLDLTVFFDGNRSVSRDIFFEKALKVIRPVYLNQST
                     IPTFYIVNVGGGYLDGDRYRMNVNVEDNAKVTLTSQGATKIYKTPSNHVEQYQTFNLK
                     DNAYLEYVADPIIAYENAKFYQHNTFNLNNSSSLFYTDILTPGYSKTGEAFKYQYMHL
                     INEIYIEDELVTYDNLLLNPNKQSINEIGYMEHYSHYGSAYFIHEDVNQKLIDSVYET
                     ISSYSNTFDCRVAISQLPTHGFAVRIFAYRTQIIEKILGTIQSYIAENIYDRKLDFLR
                     KY"
     gene            complement(5122..5736)
                     /gene="ureG"
                     /locus_tag="P6L01_001363"
     CDS             complement(5122..5736)
                     /gene="ureG"
                     /locus_tag="P6L01_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HDP3030437.1"
                     /translation="MANPIKIGIGGPVGAGKTQLIEKVVKRLSKEMSIGVITNDIYTK
                     EDEKILVNSGVLPESRIIGVETGGCPHTAIREDASMNFAAIDELLERHDDIELIFIES
                     GGDNLAATFSPELVDFSIYIIDVAQGEKIPRKGGQGMIKSDFFVINKTDLAPYVGASL
                     EQMAEDTKVFRGKRPFTFTNLKTDEGLDEVIDWIERDTLLKGLS"
     gene            complement(5749..6438)
                     /locus_tag="P6L01_001364"
     CDS             complement(5749..6438)
                     /locus_tag="P6L01_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HDP3030438.1"
                     /translation="MIDHTHLRLFQFCDSQFPTGAFSHSFGLETYIQRNIIHDDHTFI
                     AWLKMFLQEQLTYSDGLAMRLVYDALENDDTQKVLHIDKLMFVQNLPKETRVGAKQMG
                     TRMVKLALELYNSPWIAWYHQQMQDKKAKLNPAICFTMLGHHLGVDIETIIDYYLYQN
                     VSSLTQNAVRAIPLGQTAGQKIVTHMIPYIEETRKQIFELKEADFGMTAPGLELNQMA
                     HENVNVRIFIS"
     gene            complement(6431..6883)
                     /gene="ureE"
                     /locus_tag="P6L01_001365"
     CDS             complement(6431..6883)
                     /gene="ureE"
                     /locus_tag="P6L01_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HDP3030439.1"
                     /translation="MIVEEIQGNIANLSNSEKQKHVEKVYLENSDLVKRIQRVVTDHG
                     TEIGIRLKQPIDLQYGDILYADDHNMIIVDVNSEDLLVIQPRTLQEMGDIAHQLGNRH
                     LPAQFTETEMLVQYDYLVEDLLKSLGIPYVREDRKVNKAFRHIGHSHD"
     gene            complement(6896..8611)
                     /gene="ureC"
                     /locus_tag="P6L01_001366"
     CDS             complement(6896..8611)
                     /gene="ureC"
                     /locus_tag="P6L01_001366"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HDP3030440.1"
                     /translation="MSFKMTQNQYTSLYGPTVGDSIRLGDTNLFAQIEKDYAVYGEEA
                     TFGGGKSIRDGMAQNPRVTRDDVNVADLVISNAVIIDYDKMVKADIGIKNGYIFAIGN
                     AGNPDIMDNVDIIIGSTTDIIAAEGKIVTAGGIDTHVHFINPEQAEVALESGITTHIG
                     GGTGASEGSKATTVTPGPWHIHRMLEAAEGLPINVGFTGKGQATNPTALIEQINAGAI
                     GLKVHEDWGATPSALSHALDVADEFDVQIALHADTLNEAGFMEDTMAAVKDRVLHMYH
                     TEGAGGGHAPDLIKSAAFSNILPSSTNPTLPYTHNTVDEHLDMVMITHHLNAAIPEDI
                     AFADSRIRKETIAAEDVLQDMGVFSMISSDSQAMGRVGEVITRTWQVAHRMKEQRGPL
                     DGDFEHNDNNRIKRYIAKYTINPAITHGISEYVGSIEPGKLADIVLWDPIFFGVKPEL
                     VVKGGLINSAVNGDANGSIPTSEPMKYRKMYGQYGGNLTSTSMTFVSKTAYENGINRA
                     LNLKRMVRPVKNIRQLSKADMKNNSATPKLDVDPQTYEVYVDGEKITSNAATELPLTQ
                     RYFLF"
     gene            complement(8608..9018)
                     /locus_tag="P6L01_001367"
     CDS             complement(8608..9018)
                     /locus_tag="P6L01_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HDP3030441.1"
                     /translation="MIPGEIITKSTEVEINNHHPETVIEVENTGDRPIQVGSHFHFYE
                     ANAALDFEREMAYGKHLDIPAGAAVRFEPGDKKEVQLVEYAGKRKIFGFRGMVNGPID
                     ESRVYRPTDENDAYAGVFGDNGAENVNKKGGKRS"
     gene            complement(9032..9334)
                     /locus_tag="P6L01_001368"
     CDS             complement(9032..9334)
                     /locus_tag="P6L01_001368"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HDP3030442.1"
                     /translation="MHFTQREQDKLMIVVAAEVARRRKARGLKLNHPEALALISDELL
                     EGARDGKTVAELMSYGRQILNKEDVMDGVEHMITDIEIEATFPDGTKLITVHHPIV"
     gene            9594..10499
                     /gene="yut"
                     /locus_tag="P6L01_001369"
     CDS             9594..10499
                     /gene="yut"
                     /locus_tag="P6L01_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HDP3030443.1"
                     /translation="MKIIDVLLKNISQVVLISNKWTGLFILIGLFVADWTIGLAAIVG
                     SIIAYTFARFINYSEAEVNDGLAGFNPVLTAIALTIFLDKSGLDIVITMIATLLTLPV
                     AAAVREVLRPYKVPMLTMPFVIVTWFTILLSGQVKFVDTSLKLMPQNIETVNFSNNDR
                     IHFIQSLFEGFSQVFIEASVIGGVCILIGILIASRKAALLAVIASLLSFIIVALLGGN
                     YDDINQGLFGYNFVLMAIALGYTFKTAINPYISTFLGVLLTVVVQLGTTTLLEPFGLP
                     ALTLPFIIVTWILLFAGIKHDKVDA"
     gene            10716..11870
                     /locus_tag="P6L01_001370"
     CDS             10716..11870
                     /locus_tag="P6L01_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDP3030444.1"
                     /translation="MNDMDNSFLITTEIQRKWIEKFKVIRDTFKAKAEYNDQHSQFPY
                     KNIEWLIKEGYGKLTLPKAYGGEGATIEDMVILQSFLGELDGATALSIGWHVSVVGQI
                     YEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGV
                     KTYTSMSKALTHIIVAAYIEELESVGFFLVDRNLPGVEIADNWDVLGMRATESHDLIL
                     NDVKVPLKHLVETEKSKAPNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIA
                     TLPTVQQNLGKMETLLLCARQFLWSTAKGYQQYKDDSQIRNPTSASKVMVMNQGLEVI
                     DLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAKSITDTF"
BASE COUNT         4118 a         1776 c         1999 g         4044 t
ORIGIN      
        1 gaaaatgtat atttaatgga taaaatatcc taatttagca taaaaaaatg ttttaataaa
       61 agtattattt gatataatcg acttatgttt tgttactgct aaaaaacatg tggcgatata
      121 attggtagtg aatattagat gttgacaatt gatttgagaa agacaaacag gagtaaatca
      181 atatatttgt atatgtgagg atgtaagatg acagataatt attcactaca tatttataaa
      241 agtatgaaaa tgagtagtgt gaaggaagcg aacttaaaaa ttatatattg gctacaaggt
      301 acaggtgtag tttcaatcaa tctacaacaa tatgatgtaa aaagaaatga tgtcaccgtt
      361 attatgttga atgatttgta tcaaattgat ggtcatgatg atagtgtatg ttgtgttgtt
      421 gaagtaagtg caaagacatt tttgaagttt atgaatgcaa actatatgat gcgtggcaag
      481 atattaacaa atgaggttgc tagcactttt agaatcttga ttaagtattt aatctacagc
      541 aaaattaaac aacagtctta taatgctaat gataagatta ttaattacat gtgtgttgaa
      601 ttaatgtcat taaatacata cgatggtagt cgtcatttag tagccgaaga agtgcacgat
      661 tatttaacaa acaaccacca taaaaagata aataggaaag atgtcattaa tcaagttagt
      721 attacaaaca aagctttaag tgacatgttt aaagcgacac cttatagtaa ttttattcaa
      781 tatttaaatc atattagact ggagcattgc ttaattgata ttttaacttc taagaagcct
      841 attgaagaaa tcgcaagtag tcatggtttt aatcattatt ctcgttttat acacctcttt
      901 aaagaaacgt atggtaacac tcctaaatta attagaaaaa gatatagtcc aacatctcat
      961 tcaattaacc attcccaatt agttgaaatt gataaaaata taattgaatt atttgataat
     1021 actaatcaaa gttcttcaag tatgcgcgag attgatatac cttttgaacc tacaaaaagg
     1081 agtcaaatgt atcaacctaa aaacatttat attaaaggaa gtaattttta ttggatggat
     1141 tattatacta ttaaacaaat cattcaaaga ttaggtgtta atcttgaaaa cttgcatata
     1201 attgttacaa tcgacacaag caatgagcta tcgattgaac aaatcacttt attacttcaa
     1261 aagatagtga attacaatgt aaatgtagta tttcgtatta aacatgaata taaggaattg
     1321 ttaacgagta ctgaaagagg caagatagaa aagctagtag ccacaatttt taatatgaca
     1381 tttgagagta atagatgtaa gattgcattt ttaattaacg acttaaatac tactgcaatt
     1441 caaaaactga aacgcttaat agataattat ataggagtat ttgaattaat atatcatctg
     1501 gaaccaaaag agttgaatac gcactatgat aaagaaattg atcatctaat agactatttt
     1561 atcttaccgc taagacaaat agaacaggtg tctattgcat gttctaaaat tatatgtgat
     1621 gtagatttga ttaatgagaa taatgactta aaagaaaaaa tcacaatgtc tgattttaat
     1681 aaggttactg aacttttgac aaattataca aaatttcgag gacttattat gcacatgtca
     1741 attataagtg aagctagcaa agaaataaat ttgactagat tatcaatatt catttttatc
     1801 ataaatatac ttaatcaatt acgagggatt gttatttatc aaaatgacac gatgattgta
     1861 acgaagttca aacatgaagt acaatgtgtc atttgtttgc cagccgcatt gttaaatgag
     1921 aattcagacg ctatacgttt atcgtgtaag ggtcttcaac aatttagcca tgctaaaact
     1981 aaacaaattg ttttaagtat agataaagat aatcatactt ccgaaaatgg tgtacttgtg
     2041 aatgatatta tgcaacaaca cagttattgg aaagctaatt ttatagaaag aaatatttat
     2101 gatcattcat taatggtctc aagcaatagt attgttcatt tgaaatatca tcttgagata
     2161 taggcatggt ttaagagagg ttttaaagat gtctaaattt aaagttaagc gtttaactgc
     2221 acacatacta atgttgaaag tcattaatga tatgttgaaa tattcatttc atctcacttt
     2281 gactcaagtg aaactattaa atctattagt taaatatgat aattctgatt ccaatagcgc
     2341 ttcaatagat aggttattaa aaatgaaaga aatccaatct aagatggcac ttcttcaaat
     2401 gttaagttat ttaaacaatc atcaatggct attgaaagga agagatcaaa gggatcaacg
     2461 taaattgact atttcgatta ataaaatgca tatagataaa atagaatata tgaataatga
     2521 gcttagtgac tatattgaac gttattttgg tcaatataca tttgatttta gttgtgacta
     2581 tgtatcgtat ttattgtcgt cacaagaatt atttcgaaat ctaaagtgtt atttaaatat
     2641 gtgtcaatta acattagaag agttatacgt tttaggaata ttaaatctgc ataaaggtca
     2701 attgacggtt aaagaattac agggtgaatt ccatcacccg atatttgcgg ttagcccgat
     2761 tttaaagtgt ttaattttga agggattagt taaaaaagag agatgtgaat tagatgaaag
     2821 acgtgttatt gtaacaatca agcgagaaaa gttttctaaa gtcactatgc ttattcaagc
     2881 atgttataat tatcttgaaa agggaataca agttaagctt aataataaat agttaatcac
     2941 ttgtatttca tatacctatg aagaatagtt tatagtttta agtgggaggg atattatgat
     3001 tttagataat gtgaatccga atgatttatt tccaactgaa aaaaagggtc cgtcagtgct
     3061 aggaataatt gagtatcaag ttcaaggtga gaatgaattt gaaggggcat ttatagcaac
     3121 aaatgaaaga ttaattatga atgtagatat gaatggccaa ttttattata gaagtattag
     3181 ttataatgaa atagagaaga tagattatga tggtcaaact ataatgttca aatttaatat
     3241 tggtaatgtc ccaatgcatg atattaaaag tgataacgtt gaaatgtttg ttgaatatgt
     3301 aaaacaacat atgatagtat aatgtcaatg tatgtaggtt gataagaaga gtttgaatga
     3361 taactcattg catttaaaga taaatggcat gcgttatgat ttaaagctct tcttttttta
     3421 tattttataa attttgtgag caagccatcc aattaatata aaaaatatca aatcaaacac
     3481 gttgatatag attaaaattt taaaagttta catatcagtt aagatacaaa atattcagac
     3541 taataatttt caatttggca aaatatctta aacatcaaat tattataaga aataaatgta
     3601 tttaaccata ttctagtaat aaaaatattg aatttttata cttatttgtt tagaatgaac
     3661 tttataacat agttggatag agtttcgatt taataaatta catgtgaacc ttgctacaac
     3721 aagatgtgca tcagaggagt ggtttaataa tgagtaaaat taatgacatt aatgatttag
     3781 tcaacgcaac atttcaagtt aagaagtttt tcagagatac aaaaaagaag ttcaatttga
     3841 actatgaaga aatttatatt ttaaatcata ttttaagaag tgagtctaac gaaatctcat
     3901 ctaaagagat tgctaagtgc tcagagttca aaccttacta tttaactaaa gctttacaaa
     3961 agctaaaaga tttaaaattg ttatcaaaga aaagaagttt acaagacgaa agaacagtta
     4021 ttgtttatgt tacagataca caaaaagcaa atattcaaaa actgatttca gaattagaag
     4081 aatacattaa aaattaaatc aaggttaatt gcgtttaata acattgaacg ataacaattt
     4141 attaaaacga agttatttat tcagcattgg gacataaaat taacttaaaa tttaaatatt
     4201 gaagatgctt taattaaagt taaagactag ccatacctta tttcagctta ttaagcttga
     4261 aacaaggtac actagtcttt ttattttaat attttcttag aaaatcaagt ttacgatcat
     4321 aaatattttc tgcgatatag ctttggatgg ttccaagtat tttctctata atttgtgtgc
     4381 gataagcaaa aattctaact gcaaagccat gtgtaggcaa ttgagaaata gcaacacgac
     4441 aatcgaatgt attgctataa gaactaatgg tttcataaac tgaatcgatt agtttttggt
     4501 taacatcttc atgtatgaag taagcagagc cataatgaga gtaatgttcc atataaccta
     4561 tttcattgat cgattgttta ttaggattca ataataaatt atcatatgtg actaactcat
     4621 cttcaatata aatttcattt attaaatgca tatattgata cttgaaggct tcgccagttt
     4681 ttgaataacc aggagttaaa atatcagtat aaaataatga actagaatta ttgagattga
     4741 acgtattgtg ttgataaaat ttagcatttt catatgcgat gattggatca gcgacatatt
     4801 ctaaatatgc gttatctttt aaattaaaag tttgatactg ctcaacatga ttggaaggtg
     4861 tcttgtatat ttttgttgca ccttgagatg tcaatgtcac tttagcgtta tcttcgacat
     4921 tcacattcat acggtaacga tctccatcta aatagccacc acctacattt actatataaa
     4981 atgtaggaat ggtagattga tttagataaa ctggacgtat cactttaagt gctttttcaa
     5041 agaaaatatc tcttgatact gatcgattgc catcgaaaaa cactgttaaa tcaagttgcc
     5101 cagtccattg ttgttcatcc attatgataa tcctttgagt aaagtgtcgc gttcaatcca
     5161 atcgataact tcatccaaac cttcgtcggt ttttaagtta gtaaaagtaa atggacgttt
     5221 accacgaaat actttagtat cttcagccat ttgttctaat gatgcaccta catagggagc
     5281 taaatcagtt ttgttaatta caaagaaatc tgacttaatc ataccttgac cacctttacg
     5341 tggaatcttt tcaccttgag caacatcgat aatatatatt gaaaagtcaa caagttctgg
     5401 gctaaatgtt gctgctaagt tatcgccacc agattctatg aaaattagtt ctatatcgtc
     5461 atgacgttct aataattcgt ctattgctgc aaagttcata gatgcatctt cacgaatcgc
     5521 agtatgagga catccaccag tttcaacacc aatgatacga ctttcaggta gaactcctga
     5581 atttactaat atcttttcgt cttcttttgt atatatatca tttgtaataa cgccgatact
     5641 catttctttt gaaagacgtt ttacaacttt ttcaattaat tgtgttttac ctgcacctac
     5701 aggaccacca ataccaattt taatcggatt tgccacaatt ataacctcct atgatatgaa
     5761 aattctaaca ttgacgttct catgcgccat ttgatttagt tctaaaccag gcgctgtcat
     5821 gccaaaatct gcttctttta attcgaaaat ctgctttctt gtttcttcta tataaggaat
     5881 catgtgagta actatctttt gaccagcagt ttgtccaagt ggaatagcac gaacagcatt
     5941 ttgagttaaa cttgaaacat tttgatataa atagtaatca ataatcgttt caatatctac
     6001 acctaaatga tggcctagca tagtaaaaca aatagctgga tttaactttg ctttcttatc
     6061 ttgcatttgt tgatgatacc aagcaatcca tgggctatta taaagttcta aagccaattt
     6121 aaccatgcga gtccccattt gttttgcacc aacacgtgtt tctttaggta agttttgaac
     6181 aaacatcagt ttatctatgt gtaatacttt ttgtgtatca tcattttcca atgcatcata
     6241 aactaaacgc atggctaaac catcagaata ggtaagttgc tcttgtaaaa acatttttaa
     6301 ccaagcaata aaagtatgat cgtcatgaat tatatttcgt tgaatatatg tttcaagacc
     6361 aaatgaatga ctgaaagcac ctgttggaaa ctgtgaatca cagaactgaa ataatcttaa
     6421 gtgtgtatga tcaatcatga gaatgcccta tatgtctgaa agccttatta actttacggt
     6481 cttctcgaac atatgggatg cctaaacttt ttaataaatc ttcaactaaa taatcatatt
     6541 gtactagcat ttcagtctct gtaaattgtg ctggcaaatg acgatttcct aattgatggg
     6601 ctatatctcc catttcttgc aatgttcttg gttgaatcac taaaagatct tctgaattaa
     6661 catccacaat aatcatatta tggtcatctg cgtataaaat atctccatat tgtaagtcaa
     6721 taggttgttt taaacgaatg cctatttcag tgccatggtc tgtaacgact ctttgaatac
     6781 gtttaacaag atctgaattt tcaaggtata ctttttcgac gtgcttttgt ttttctgaat
     6841 ttgacaaatt ggcaatattg ccttggattt cttcaacaat cattctatgt tcctcctaga
     6901 ataagaagta tctttgagtt aatggtaact cagttgctgc attacttgta attttttctc
     6961 catctacata tacttcatat gtttgtggat caacgtctaa tttaggtgtt gcactgttat
     7021 ttttcatatc tgctttagat aattgtctaa tatttttaac tggacgcacc atgcgtttta
     7081 aatttaatgc acgattgata ccattttcat aagcagtttt agacacgaat gtcattgacg
     7141 tacttgtaag gtttccgccg tattgaccat acattttacg gtacttcatc ggttcagatg
     7201 taggtataga accatttgca tcgccattta cggcagagtt aattaatccg ccctttacaa
     7261 ctaattcagg tttaacccca aagaaaattg ggtcccataa gacaatgtca gctagtttgc
     7321 ccggctcgat agatcctaca tattcagaaa taccatgtgt aattgctggg ttaattgtat
     7381 atttagcgat ataacgtttg atgcgattat tatcattatg ttcaaaatca ccatctaaag
     7441 gaccacgttg ttctttcatg cgatgtgcta cttgccatgt tcgtgtaatt acttcaccta
     7501 cacggcccat tgcttgtgaa tcggaactaa tcatactgaa tacacccata tcttgcagaa
     7561 catcttctgc tgcaatcgtt tctttacgaa tacgtgaatc tgcgaatgcg atatcttcag
     7621 gaatagccgc atttaaatgg tgagtaatca ttaccatatc taaatgttca tctacagtat
     7681 tatgtgtata aggcaaagtt ggatttgtag atgaaggtaa aatatttgaa aatgcagcgg
     7741 atttaattaa atcaggcgca tgaccgccac cagcaccttc agtatggtac atatgaagta
     7801 cacggtcttt aacagcagcc attgtgtctt ccataaatcc tgcttcattt aaagtatctg
     7861 catgtaatgc aatttgaaca tcaaattcat cagcaacatc taatgcatga ctcaaagcag
     7921 atggtgttgc accccagtct tcatgtactt ttaatccaat tgctccggca ttgatttgtt
     7981 caatgagtgc agttggattt gttgcttgtc ctttacctgt aaaaccgaca ttaatcggta
     8041 aaccttcggc agcttctaac attctatgaa tatgccatgg acctggagtt acagttgttg
     8101 ctttagaacc ttctgaagca ccagtaccac caccaatatg agtcgtaata ccactttcta
     8161 atgcgacctc tgcttgttca ggattaataa aatgaacatg agtatcaata ccaccagcag
     8221 tgacgatttt accttcagcg gcaatgatat ctgttgttga acctataata atgtcgacat
     8281 tatccattat atctgggttg ccggcattac ctatggcgaa aatataacca tttttaatgc
     8341 ctatatcagc tttaaccatt ttatcgtaat cgataataac ggcattagaa atgacaaggt
     8401 ctgcaacgtt cacgtcatca cgtgttacac gaggattttg cgccataccg tctctaatag
     8461 atttaccacc accaaaagta gcttcttcac cataaaccgc atagtctttt tctatttgag
     8521 caaatagatt cgtatcacct aaacgaatag aatctccaac agttggaccg tataagctcg
     8581 tatattgatt ttgcgtcatt ttaaagctca tgatcttttt cctccttttt tattcacgtt
     8641 ttcagcaccg ttatctccga atacacctgc atatgcatca ttttcatcag ttgggcgata
     8701 gacacgtgac tcatcgatag gaccattgac cataccacga aaaccaaaaa ttttacgttt
     8761 gccagcatat tcaactaatt gaacttcttt tttatcccca ggttcaaatc gaacagctgc
     8821 tccagctgga atatctaaat gttttccata tgccatttca cgttcgaaat ctaatgctgc
     8881 atttgcttca taaaaatgaa aatgtgagcc cacttgaatt ggtcgatctc ctgtattttc
     8941 aacttcgata actgtttcag gatgatggtt attaatttca acctctgtac tttttgtaat
     9001 aatttctcct ggtatcattt gactgcctcc tttaaacaat agggtgatgt actgtgatta
     9061 acttagtacc atcggggaac gtagcctcga tttcgatatc tgtaatcatg tgttcgacac
     9121 catccatgac atcttctttg tttagaattt gtctaccata actcattaac tctgcaacgg
     9181 tcttaccatc gcgtgcacct tctaataatt catcgctgat taaagctaat gcctcaggat
     9241 gatttagttt caaaccacgt gctttacgac gacgtgcaac ttccgccgcc actacaatca
     9301 ttaatttgtc ttgctctcgt tgtgtaaaat gcaaattaaa acccccaatt tcatattaga
     9361 tacaatttac aaaatttata ttaatcctaa ttgttgtgat aaacaagtaa tatacaaagt
     9421 tcaatgtgta attagaaaat tatattttta gcatatccgc tattgaagca aacaatctaa
     9481 tcgaaaacaa atagcggaat atatttatgt aaaaaccaaa atagttttta atataacttt
     9541 tcatagaata gtagtatatt aatgagtaat gattcaaagg aaaggtgaaa gatttgaaga
     9601 taatagatgt gcttttgaaa aatatatctc aggttgtgtt aattagtaat aaatggacag
     9661 gattatttat cttaatagga ttatttgtag ccgattggac aattggatta gcggctattg
     9721 taggtagcat catcgcctat acttttgcgc gttttataaa ttatagtgag gcagaggtta
     9781 atgatgggtt agccggattt aatccagtgt taactgccat tgcgttaaca atctttttag
     9841 ataagtcagg attagatatt gttataacaa tgatagcaac tttattaacg ttaccagttg
     9901 ctgctgcagt gagagaagtt ttaagaccat ataaagttcc gatgctgacg atgccttttg
     9961 tcattgtgac ttggtttaca attttacttt caggacaggt taaatttgta gatacatcgt
    10021 taaagttaat gcctcaaaac attgaaacgg ttaattttag caacaatgat agaatacatt
    10081 tcattcagtc attatttgaa ggattcagtc aagtatttat cgaagcgagt gtaattggtg
    10141 gcgtatgtat tttaatcggc atattgatag catcaagaaa agcagcactc ttagctgtta
    10201 tagctagttt gttaagcttt atcattgtag ctctattagg tggtaattat gatgatatta
    10261 atcagggatt attcggttat aactttgtat taatggcaat cgcactagga tatacattta
    10321 aaacagcgat taacccttat atttcgactt ttttaggtgt gttattaaca gtagtggtgc
    10381 aactaggtac aacaacattg cttgaaccgt ttggcttacc tgcattaaca ttgccattta
    10441 ttatcgtgac atggatttta ttatttgctg gtattaaaca tgacaaagta gatgcttgat
    10501 agttaaatca aacctaatat tgtttgaata tcaccttaaa ccatacagcg aattgtatag
    10561 tttaaggtgt atttttatgg ataaaattaa gtgcatactt aatcgaatta tcagttttct
    10621 tttaattgtt cacagcacgt tcctttttat ttttaaagtc gagtgttaaa ctaggaataa
    10681 ataatttata ttgtgtgttg tgtggggtga ctaatatgaa tgatatggat aattcctttt
    10741 taataacaac ggaaattcaa agaaaatgga ttgaaaaatt caaagtaatt agagatacat
    10801 ttaaggctaa agctgaatat aatgatcaac atagccaatt tccatataaa aatattgaat
    10861 ggttaattaa agaaggttat ggaaaattaa cgttaccaaa agcatatggt ggtgaaggtg
    10921 cgaccataga agacatggtt attttgcaat catttttagg cgaacttgat ggtgccacag
    10981 cattatctat tggttggcat gtgagtgtcg taggacaaat ttatgaacag aaattatggt
    11041 ctcaagatat gttggagcaa tttgctgttg aaattaataa tggtgcatta gttaatagag
    11101 cagttagtga agctgaaatg ggtagtccaa caagaggggg aagaccaagt acacatgctg
    11161 ttaaagctga tgatgggtat attttaaatg gtgtgaagac atatacatca atgagtaaag
    11221 cactaacaca tattattgtt gctgcttata tagaagaatt agaaagtgtt ggttttttct
    11281 tagtagacag gaatttgcct ggtgttgaaa ttgccgataa ttgggacgta ttaggtatgc
    11341 gagcgacaga aagtcatgat ttaatattaa atgatgtaaa agtaccatta aaacatttag
    11401 tggaaacaga gaaaagtaaa gcgccaaatg gttggatatt gcatatacca agctgttatt
    11461 taggtatagc acaagctgct agaaattatg ctgtagactt tgcaatccaa catagcccta
    11521 atagtattga aggaacgatt gcaacattgc caactgtgca acaaaattta gggaaaatgg
    11581 aaacgctatt gttatgtgcg agacaatttt tatggagtac agcaaaaggg tatcaacaat
    11641 ataaagatga cagtcagata agaaatccaa caagtgcgag taaagtgatg gtaatgaatc
    11701 aagggcttga agtgattgat ttagcgatga gaatagttgg tgctaaaagc ttagaaatga
    11761 atcgaccatt acaacgttac tatcgtgaca tgcgtgcagg attacacaat ccacctatgg
    11821 aagatgcagc atatacaaat attgctaaga gtattacaga tacattttaa atttaaaaag
    11881 gtaaatcgat cgttgattgg cataaagaga tgaaatttga cgttcggatt taccctt
//
LOCUS       DAOBBH010000069        11921 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.069, whole genome shotgun sequence.
ACCESSION   DAOBBH010000069 DAOBBH010000000
VERSION     DAOBBH010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11921
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.069"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            25..2121
                     /gene="pnp"
                     /locus_tag="P6L01_001371"
     CDS             25..2121
                     /gene="pnp"
                     /locus_tag="P6L01_001371"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDP3030445.1"
                     /translation="MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLST
                     ATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPL
                     FPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGK
                     YIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDF
                     QQQIVDHIQPVKQEFIPAERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKE
                     EIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSE
                     VGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVG
                     ETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGS
                     TLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITA
                     IQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKP
                     DKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAE
                     VGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITE
                     IDKQGRVNASHRALEE"
     gene            2357..4030
                     /locus_tag="P6L01_001372"
     CDS             2357..4030
                     /locus_tag="P6L01_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDP3030446.1"
                     /translation="MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMF
                     PEDEMLGIDIVIPDISYVLENKDKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLT
                     IALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGA
                     IVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHM
                     YDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFD
                     IPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAI
                     TASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQ
                     GEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNQKVNSGNILIDGIGIG
                     DVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEE
                     KVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI"
     gene            4287..6653
                     /locus_tag="P6L01_001373"
     CDS             4287..6653
                     /locus_tag="P6L01_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDP3030447.1"
                     /translation="MAQAKKKSTAKKKTASKKRTNSRKKKNDNPIRYVIAILVVVLMV
                     LGVFQLGIIGRLIDSFFNYLFGYSRYLTYILVLLATGFITYSKRIPKTRRTAGSIVLQ
                     IALLFVSQLVFHFNSGIKAEREPVLSYVYQSYQHSHFPNFGGGVLGFYLLELSVPLIS
                     LFGVCIITILLLCSSVILLTNHQHRDVAKVALENIKAWFGSFNEKMSERNQEKQLKRE
                     EKARLKEEQKARQNEQPQIKDVSDFTEVPQERDIPIYGHTENESKSQCQPSRKKRVFD
                     AENSSNNIVNHQADQQEQLTEQTHNSVESENTIEEAGEVTNVSYVVPPLTLLNQPAKQ
                     KATSKAEVQRKGQVLENTLKDFGVNAKVTQIKIGPAVTQYEIQPAQGVKVSKIVNLHN
                     DIALALAAKDVRIEAPIPGRSAVGIEVPNEKISLVSLKEVLDEKFPSNNKLEVGLGRD
                     ISGDPITVPLNEMPHLLVAGSTGSGKSVCINGIITSILLNAKPHEVKLMLIDPKMVEL
                     NVYNGIPHLLIPVVTNPHKAAQALEKIVAEMERRYDLFQHSSTRNIKGYNELIRKQNQ
                     ELDEKQPELPYIVVIVDELADLMMVAGKEVENAIQRITQMARAAGIHLIVATQRPSVD
                     VITGIIKNNIPSRIAFAVSSQTDSRTIIGTGGAEKLLGKGDMLYVGNGDSSQTRIQGA
                     FLSDQEVQDVVNYVVEQQQANYVKEMEPDAPVDKSEMKSEDALYDEAYLFVVEQQKAS
                     TSLLQRQFRIGYNRASRLMDDLERNQVIGPQKGSKPRQVLIDLNNDEV"
     gene            6658..7371
                     /locus_tag="P6L01_001374"
     CDS             6658..7371
                     /locus_tag="P6L01_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDP3030448.1"
                     /translation="MSEMNAVYNVKQYILNLIKQNKLEYGDQLPSNLSIARELNVKTD
                     DVYEAIQALITEQVIKDNFEEGTSVKSLPPFFYPLNELISIGQMIKNAGFECGTEYLN
                     FDEQPATMLDANLLSVEEGYPVTIIERLRTADGEPVVYCLDKIAKKELTCTEYQMSNG
                     SILSAIKEQSNHNICYADTEIEAVNYEPRISEVLNASPHEGLILLKITHYNELDEPIL
                     YSLNYMKNSLVQFKITRKI"
     gene            7402..8667
                     /locus_tag="P6L01_001375"
     CDS             7402..8667
                     /locus_tag="P6L01_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDP3030449.1"
                     /translation="MSRQSQPNIHIKVSPTTKFKTTTIVFKFMAPLEYDTITARSLLS
                     KLLVRATKKWPTDKSFNNHLADLYGAYVNSTISKFKDQHVITFSLEIVNERYLRNGES
                     LFNQGLDLLQEIIWNPLIENKAFNDNFVNQEKTLLAKKIEAMVDNKAQYSFLKLLDHM
                     FENEAYKYLSTGQLEQIPHITAETLYHTYQSMINNDQCSVYVVGNVEPESVEKQIREK
                     FALKPFDKHQFQHSTHHLHDEEVDYIVEYDDVDQAKLNVGYRFPTQYGQSGYAAFVVF
                     NMMFGGDPSSVLFNEVREKQSLAYSIHSQIDGKNGYLFVLSGVSSDKYETAKDTIISE
                     FEKIKAGDFTEEKLELAKKVIISHRYESEDRPKSIIEIMHNQILLEQPQSKETFINDI
                     QKVSREDIVSVAEKAFLDTIYVLTKGGDK"
     gene            8667..9953
                     /locus_tag="P6L01_001376"
     CDS             8667..9953
                     /locus_tag="P6L01_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDP3030450.1"
                     /translation="MKERYYELIDERVFEQELENGLRLFIIPKPGFQKTFVTYTTQFG
                     SLDNQFKPLGQDQFVTVPDGVAHFLEHKLFEKEEEDLFTAFAEDNAQANAFTSFDRTS
                     YLFSATDNIENNIKRLLTMVETPYFTKETVDKEKGIIAEEIKMYQEQPGYKLMFNTLR
                     AMYQRHPIRVDIAGSVESIYDITKDDLYLCYETFYHPSNMVLFVVGDVNPEEICRIVK
                     QHEDARNKVNQPKIERGLVDEPEDVKEAFVTESMKIQSPRLMLGFKNKPLQEAPQKYV
                     QRDLEMSLFFELIFGEETDFYQNLLNEGLIDDTFGYQFVLEPTYSFSIVTSATEEPDK
                     LKKLLLDELRDKKGNFQDAEAFELLKKQFIGEFISSLNSPEYIANQYTKLYFEGVSVF
                     DMLDIVENITLDSINETSSLYLNLDQQVDSRLEIKK"
     gene            9953..10657
                     /locus_tag="P6L01_001377"
     CDS             9953..10657
                     /locus_tag="P6L01_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDP3030451.1"
                     /translation="MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFN
                     DDKVRFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNV
                     LQLIRLCRYFVDVLRQSANGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQEL
                     ALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHSNA
                     RSVTGTIQKVNGAWYI"
     gene            10762..11589
                     /locus_tag="P6L01_001378"
     CDS             10762..11589
                     /locus_tag="P6L01_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfK family protein"
                     /protein_id="HDP3030452.1"
                     /translation="MTVAEKKEWYLEYEIEINRPGLLGDVSSLLGMLGISIVTINGVD
                     QGKRGLLIKTDNLEKVERFEQIARGINEIEITKLKKPELRDRLAVRHGRYIEQDAKDK
                     KTFRFEREDLGLLVDFLAELFKEEGHKLIGIRGMPRVGKTESIVAGSVCAHKRWLFIS
                     STLIKQTVRSSLIKGEYDANHVYIIDGAVTARESNPKHQELVNEVMTLPSIKVVEHPD
                     LFVETSTCTMEDFDYIIELRENENQEIHYEEMKKQTVQSKNNLDFGDPFGGGFGFFE"
     gene            11608..>11921
                     /locus_tag="P6L01_001379"
     CDS             11608..>11921
                     /locus_tag="P6L01_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDP3030453.1"
                     /translation="MKTVGEALKGRRERLGMTLTELEQRTGIKREMLVHIENNEFDQL
                     PNKNYSEGFIRKYASVVNIEPNQLIQAHQDEIPSNQAEWDEVITVFNNNKDLDYKSKS
                     KEP"
BASE COUNT         4363 a         1628 c         2325 g         3605 t
ORIGIN      
        1 tagattcaag agaggagatt tataatgtct caagaaaaga aagtttttaa aactgaatgg
       61 gcaggaagat ctttaacgat tgaaacaggg caattagcta aacaagcaaa tggcgctgta
      121 ttggttcgtt atggagatac agtcgtgtta tcgacggcaa ctgcatcaaa agaacctcgt
      181 gatggagatt tcttcccatt aacagtgaac tacgaagaaa aaatgtacgc tgcgggtaaa
      241 attcctggtg gatttaaaaa gagagaagga cgtcctggtg acgatgcaac attaactgcg
      301 cgattaattg atagaccaat tagaccttta ttccctaaag ggtataagca tgatgttcaa
      361 attatgaaca tggtattaag tgcagatcct gattgttcac cacaaatggc tgcaatgatt
      421 ggttcatcta tggcgcttag tgtgtcggat attccattcc aagggccaat cgccggtgta
      481 aatgtgggtt atattgatgg taaatatatc attaacccaa cagtagaaga aaaagaagtt
      541 tctcgtttag accttgaagt agctggtcat aaagatgcag taaacatggt agaggcaggc
      601 gctagtgaga ttactgaaca agaaatgtta gaggcgattt tctttggtca tgaagagatt
      661 caacgtttag ttgatttcca acaacaaatc gtcgaccaca ttcaacctgt taaacaagaa
      721 tttattccag cagagcgtga tgaagcgcta gttgaacgtg tgaaatcttt aaccgaagaa
      781 aagggactta aagaaacagt tttaacattt gataaacaac aacgagatga aaatcttgat
      841 aacttaaaag aagaaatcgt caatgaattt atcgatgaag aagatccaga gaatgaatta
      901 cttattaaag aagtttatgc aattttaaat gaattagtga aagaagaagt tcgacgttta
      961 attgcagatg aaaaaattag accagacggc cgtaaacctg atgaaatccg tccattagat
     1021 tctgaagttg gtattttacc tagaacgcat ggttcaggtc tatttacacg tggtcagact
     1081 caagcacttt cagttttaac attaggtgct ttaggcgatt atcaattaat tgatggttta
     1141 ggacctgaag aagaaaaaag attcatgcat cattataact tcccgaactt ttcagtaggt
     1201 gaaactggtc cagtacgtgc gccaggtcgt cgtgaaattg gacatggtgc gttaggtgaa
     1261 agagcattaa aatatattat tcctgatact gctgatttcc catatacaat tcgtattgta
     1321 agtgaggtac ttgaatcaaa cggttcatca tctcaagcgt caatttgtgg atcaacctta
     1381 gcattaatgg atgcgggcgt accgattaaa gcaccagttg ctggtattgc tatgggcctt
     1441 gttacacgtg aagatagcta tacgatttta actgatatcc aaggtatgga agatgcatta
     1501 ggtgatatgg actttaaagt cgctggtact aaagaaggta ttacagcaat ccaaatggat
     1561 attaaaattg acggtttaac gcgtgaaatt atcgaagagg ctctagaaca agcgagacgt
     1621 ggtcgtttag aaataatgaa tcatatgtta caaacaattg atcaaccacg tactgaatta
     1681 agtgcttacg cgccaaaagt tgtaactatg acaattaaac cagataagat tagagatgtt
     1741 atcggacctg gtggtaaaaa aattaacgaa attattgatg aaacaggcgt taaattagat
     1801 attgaacaag atggtactat ctttattggg gccgttgatc aagctatgat aaatcgtgct
     1861 cgtgaaatca ttgaggaaat tacacgtgaa gcggaagtag gtcaaactta tcaagccact
     1921 gttaaacgta ttgaaaaata cggtgcgttt gtaggcctat tcccgggtaa agatgcgttg
     1981 cttcacattt cacaaatttc aaaaaataga attgaaaaag tggaagatgt tttaaaaatc
     2041 ggtgacacaa ttgaagttaa gattactgaa attgataaac aaggtcgagt aaatgcatca
     2101 cacagagcat tagaagaata atatttaaag tcatatgacg acaatgtatc gtcatgtgat
     2161 ttttttatgc cattttttac gaagtgatcc attttgaatt tgttgtattg aacattttaa
     2221 aacgctttat tattttgagt gcaactgtta attatcctgt atgtatagtg attaatagtg
     2281 tacatcaagt gttttttaac ttataatgaa tagtgagttt atatatggac gggtaacaaa
     2341 tttaggaggt aagattttga gtttaataaa gaaaaagaat aaagatattc gcattatacc
     2401 attaggcggt gttggcgaaa ttgctaaaaa tatgtatatc gttgaagtag acgatgaaat
     2461 gtttatgtta gatgctggac ttatgtttcc agaagacgaa atgctaggta ttgatattgt
     2521 tataccagac atttcatacg tacttgaaaa taaagataaa ttgaagggta tattccttac
     2581 acacggacat gagcacgcga ttggtgcagt gagttatgtt ttagaacaat tagatgcacc
     2641 agtatatgga tctaaattga caatagcgtt aattaaagaa aatatgaaag cccgtaatat
     2701 tgataaaaaa gttcgctact acacagttaa caatgattca attatgagat tcaaaaacgt
     2761 gaatattagt ttctttaata cgacacacag tattcctgat agtttaggtg tttgtattca
     2821 cacttcatat ggtgccattg tgtatacagg tgaatttaag tttgaccaaa gtttacatgg
     2881 acattatgca ccagatatta aacgtatggc agagattggt gaagaaggcg tatttgtctt
     2941 aatcagtgat tctactgagg cagagaaacc tggatataat actccggaaa atgtaattga
     3001 acatcatatg tatgatgctt ttgcaaaagt gcgaggtcgc ttgattgttt catgttatgc
     3061 ttcgaacttt atacgtattc agcaagtttt aaatattgct agcaagctaa atcgtaaagt
     3121 gtcattttta ggaagatcac ttgaaagttc atttaatatt gctcgtaaaa tggggtattt
     3181 cgacattcct aaagatttgc taattcctat aacagaagtt gataattatc ctaaaaatga
     3241 agtgataatt atagctactg gtatgcaagg agaacctgta gaagccttaa gtcaaatggc
     3301 gcaacataag cataaaatta tgaatatcga agaaggcgat tctgtatttt tagcaattac
     3361 ggcttctgct aatatggaag ttatcattgc gaatacatta aatgagcttg tacgtgctgg
     3421 cgcacatatt attccaaata acaagaagat tcatgcttca agtcatggtt gcatggaaga
     3481 attaaaaatg atgattaata ttatgaaacc tgaatacttt attcctgtac aaggtgaatt
     3541 taaaatgcag atagcacatg cgaagctagc agctgaagca ggtgttgcac cggaaaagat
     3601 tttccttgtg gaaaaaggag atgtcattaa ttacaacggt aaagatatga tattaaatca
     3661 aaaggtaaat tcgggaaata ttttaataga tggtattggt attggggatg taggaaatat
     3721 cgtgttgaga gaccgtcatc ttttagcaga agatggtatc tttattgctg tagtaacgtt
     3781 agatcctaaa aatagacgta tagctgcggg tcctgaaatt caatctcgtg ggtttgtata
     3841 tgtacgtgaa agtgaagact tattacgtga agcagaagag aaagtacgtg aaatagtaga
     3901 agctggttta caagaaaaac gcatagaatg gtctgaaatt aagcaaaata tgcgtgatca
     3961 aattagtaaa ctattattcg aaagtacaaa acgtcgtcct atgattattc cagtaatttc
     4021 tgaaatttaa tcaaaaagtc attaacataa aagaggtcag aacaagtcac cgaaatataa
     4081 tggttgtcat ggacaattta cttatatttt atgatggtca attgaaaggg gtaacgatta
     4141 atctgttatc ttaagtaaat tgatacatag atgatattgt tctaacctct ttcatcgtct
     4201 gtttggacta catattctaa acatcaaata ggaaattata tataataacg tcgttttaac
     4261 taaggcaaca taaggaggtg cgtcaattgg cacaagcaaa aaagaaatcg acagctaaga
     4321 aaaaaacagc atcaaaaaaa agaacaaatt caaggaaaaa gaagaatgat aatccgatac
     4381 gttatgtcat agctatttta gtagttgtat taatggtgtt gggtgttttc caattaggaa
     4441 taatcggtcg tctaattgac agcttcttta attatttatt tgggtacagt agatatttaa
     4501 catatatttt agtactctta gcaactggtt ttattacata ctctaaacgt attcctaaaa
     4561 ctagacgaac ggctggttcg attgtattgc aaattgcatt gctatttgta tcacagttag
     4621 tttttcattt taatagtggt atcaaagctg aaagagaacc tgtactttct tatgtatatc
     4681 agtcatacca acacagtcat tttccaaatt ttggtggcgg tgtattaggt ttttatttat
     4741 tagagttaag cgtaccttta atttcattat ttggtgtatg tattattact attttattat
     4801 tatgctcaag tgttatttta ttaacaaacc atcaacatcg tgatgttgca aaagttgcac
     4861 tggaaaatat aaaagcttgg tttggttcat ttaatgaaaa aatgtcggaa agaaaccaag
     4921 aaaaacaatt gaagcgtgaa gaaaaagcga gacttaaaga agaacaaaag gcacgtcaaa
     4981 atgaacagcc acaaataaaa gatgtgagtg attttacgga agtgcctcaa gaaagagata
     5041 ttccaattta tgggcatact gaaaatgaaa gtaaaagcca gtgtcaacca agtcgaaaaa
     5101 aacgagtgtt tgatgcagag aatagttcga ataacatcgt aaatcatcaa gcagatcagc
     5161 aagagcaatt aacagaacaa actcataaca gtgttgaaag tgaaaacact attgaagaag
     5221 ctggtgaagt tacgaatgta tcgtatgttg ttccaccgtt aactttactt aatcaacctg
     5281 caaaacaaaa agcaacatct aaagctgaag tacaacgtaa aggacaagta ctagagaata
     5341 cattaaaaga ttttggggta aatgcaaaag tgacacaaat taaaattggt cctgcagtaa
     5401 ctcaatatga aattcaacca gctcaagggg ttaaagtgag taaaattgta aacttgcata
     5461 atgatattgc attagcttta gcagcaaaag atgttagaat cgaagcacca atacctggtc
     5521 gctctgcagt aggtattgaa gtgccaaatg agaaaatttc attagtttca ctaaaagaag
     5581 ttttagatga aaaattcccg tctaataata aactagaagt tggattagga agagatatat
     5641 caggtgatcc aattactgtt ccactaaatg aaatgccaca cttattggtg gcaggatcga
     5701 cgggtagtgg taaatctgtt tgtataaatg gtattattac aagtatttta ttaaatgcta
     5761 agccgcatga agttaaactt atgttaatcg atccgaaaat ggttgaacta aatgtttata
     5821 acggaattcc acatttatta attccggttg ttacaaatcc tcataaagct gctcaagctt
     5881 tagaaaaaat tgtagctgag atggaaagac gttatgattt attccaacat tcatcaacta
     5941 gaaatattaa aggttataac gaattaatcc gtaagcaaaa tcaagaatta gatgagaagc
     6001 aaccagaatt accttatatc gttgttattg tagatgagct tgcagattta atgatggtag
     6061 ctggtaaaga agttgaaaat gcgattcaac gtatcacaca aatggcacgt gcagcaggta
     6121 tacatttgat tgtagcaaca caaagacctt ctgtggatgt aattacaggt atcattaaaa
     6181 ataacattcc atctagaatt gcttttgctg tgagttctca aacagattca agaactatta
     6241 ttggtactgg cggcgcagaa aagttacttg gtaaaggtga catgttatac gttggaaatg
     6301 gtgattcatc acaaacacgt attcaagggg cgtttttaag tgaccaagag gtgcaagatg
     6361 ttgtaaatta tgtagtagaa caacaacagg caaattatgt aaaagaaatg gaaccagatg
     6421 caccagtgga taaatcggaa atgaaaagtg aagatgcttt atatgatgaa gcgtatttgt
     6481 ttgttgttga acaacaaaag gcaagtacat cattgttaca acgccaattt agaattggtt
     6541 ataatagagc atctaggttg atggatgatt tagaacgcaa tcaggtaatc ggtccacaaa
     6601 aaggaagcaa gcctagacaa gttttaatag atcttaataa tgacgaggtg taaaaaaatg
     6661 tcagaaatga atgcggtata taacgttaaa caatacattt taaatttgat taagcaaaat
     6721 aaattggaat atggtgacca acttccaagt aatttatcaa ttgccagaga attaaatgta
     6781 aaaaccgacg atgtttatga agcaattcag gcattgatta ctgaacaagt cattaaagat
     6841 aattttgaag agggcacaag tgttaaatca ctgccccctt tcttttatcc attgaatgaa
     6901 cttataagta ttgggcaaat gattaaaaat gcaggatttg aatgcggaac tgaatactta
     6961 aattttgatg agcaaccagc aactatgtta gatgcaaatt tgttgagcgt tgaagaagga
     7021 tatccagtaa ccattataga acgattacga actgccgatg gagaaccggt cgtctattgt
     7081 ttagataaaa ttgctaaaaa agaattaaca tgtacagagt atcaaatgag caatggatcg
     7141 atactaagtg cgataaaaga acaaagtaat cataatattt gttacgctga tacagaaatt
     7201 gaagcggtaa attatgaacc tcgaatatcc gaagtactga atgcttcgcc acacgaaggt
     7261 ttgattttat taaaaattac gcactacaat gaattagatg aacctatttt gtattcatta
     7321 aattatatga aaaatagctt agttcaattt aaaatcacta gaaaaatata gatgtaagaa
     7381 gatatagagg aggctatata tttgagtaga caatctcaac caaatataca tatcaaagtt
     7441 tcaccaacaa ctaaatttaa aacaactact atagttttta aatttatggc acctttagaa
     7501 tatgacacaa ttacagctag atcattatta agcaaattat tagttcgagc aactaagaaa
     7561 tggccaaccg ataagtcgtt taataatcac ttagccgatt tatatggtgc gtatgtgaat
     7621 agtacaattt caaaattcaa agatcagcat gtcattacat tttcattaga aattgttaat
     7681 gaacgttatt taagaaacgg tgaatcatta tttaatcaag gattagattt attacaagaa
     7741 atcatttgga atccattaat tgaaaataaa gcattcaatg ataattttgt taaccaagag
     7801 aaaacattat tagccaaaaa aatagaagca atggtagata ataaagcaca atattcgttt
     7861 ttaaaattac tcgaccatat gtttgaaaat gaagcatata aatacttatc tacaggacaa
     7921 ctagaacaaa tcccacatat tactgctgaa acactatatc atacatatca atcaatgatt
     7981 aataatgatc aatgttctgt ttatgttgtc ggcaatgtag aacctgaaag tgttgagaaa
     8041 caaatacgtg aaaaatttgc acttaaacca ttcgataaac atcaattcca acattctact
     8101 catcatttac acgatgaaga agttgattat attgttgaat atgatgacgt ggatcaagct
     8161 aaattaaatg tgggataccg ttttccaaca caatatggac aaagtggata tgctgcgttt
     8221 gttgtattta acatgatgtt tggaggagat ccttcatctg ttttatttaa tgaagtgcga
     8281 gaaaagcaaa gtttagcgta ctctatacat tcacaaattg atggcaaaaa tggctattta
     8341 tttgttttga gtggggtttc aagtgataag tacgaaactg caaaagacac tattataagt
     8401 gaatttgaaa aaataaaagc aggagatttc actgaagaaa aattagagtt agctaaaaaa
     8461 gtaatcattt ctcatcgata tgaatctgaa gatcgtccga aaagtattat agagattatg
     8521 cataaccaaa tattattaga gcaaccacaa agcaaagaaa catttataaa tgatatacag
     8581 aaggtaagtc gcgaagatat tgtttctgtt gctgaaaaag catttttaga tacaatctat
     8641 gtgttgacaa aaggagggga taaataatga aagagcgtta ttatgaatta atagacgaaa
     8701 gagtattcga acaagaatta gaaaatggtt tacgattatt tattatcccc aaaccaggtt
     8761 ttcaaaagac atttgtcact tacactacac aatttggttc attagataat caattcaaac
     8821 cccttggaca agaccaattt gttactgtac ctgatggagt tgctcacttt ttagaacata
     8881 aattatttga aaaagaagaa gaagacttat ttactgcgtt tgctgaagat aatgcacaag
     8941 caaatgcgtt tacaagcttt gatcgtacaa gctacttgtt cagtgcaact gataatattg
     9001 aaaacaacat taaacgttta cttacaatgg ttgaaacgcc ttattttaca aaagaaactg
     9061 ttgataaaga aaaaggtatt attgcagaag aaataaaaat gtatcaagaa caacctggat
     9121 ataaattaat gtttaataca ttgcgtgcaa tgtatcaacg acatccaata cgtgttgata
     9181 ttgccggtag tgtagaaagt atatacgata ttacaaaaga tgatttgtat ctatgttatg
     9241 aaacgtttta tcatccatca aatatggttt tatttgttgt tggtgatgtg aatccagaag
     9301 aaatatgtcg aatagtaaaa caacacgagg atgctcgtaa taaagttaac caacccaaaa
     9361 tcgaacgagg acttgttgat gaaccggagg atgttaaaga agcatttgtt actgaatcta
     9421 tgaaaattca atcaccaaga ctaatgcttg gttttaaaaa taaaccatta caagaagcgc
     9481 ctcaaaaata tgtacaacgt gatttagaaa tgtcattatt ctttgagtta atttttgggg
     9541 aagaaacaga tttttatcag aatttattaa acgaaggact tatcgatgat acatttggtt
     9601 atcaatttgt actagagccg acgtatagtt tttcaatcgt gacaagcgct actgaagaac
     9661 cagataaatt aaaaaaatta ttattagatg agttgcgtga taaaaaaggc aatttccaag
     9721 atgcagaagc atttgaactt ttgaaaaagc aatttatagg tgaattcata tcaagtttaa
     9781 actcacctga atatattgct aatcaatata ctaaattgta ttttgaaggt gttagcgtgt
     9841 ttgacatgtt agacattgtt gaaaatatca cgttagatag tattaacgaa acgtcatcgt
     9901 tatatttaaa tttagatcag caagtcgata gtcgtttgga gattaaaaag taatgaaagc
     9961 attagtatta ggtggttctg gttcaattgg ttctgagata gtcaaacaat tattaactga
    10021 tggatttgaa gtttatgtgc aatattatcg tactgatata aatgaattaa ctagcaaatt
    10081 taatgatgat aaagttcgtt ttatacaagc ggatttatct caaacaattg atattgacaa
    10141 aacatttggt gacattaaat cattagactg tttaatatat gcaagtggtc agtctttata
    10201 tggtgtttta caagatatga aagaccatga tattgatgca tgttatcagt taaatgtctt
    10261 gcaattaatt cgattatgta gatatttcgt tgatgtttta cgtcaaagtg ccaatggaag
    10321 aattattgta atttcatcaa tttggggtga gacaggagct agtatggaaa ctatttattc
    10381 ggcgatgaaa agtgcacaat taggtttcgt taaggcgctt agtcaagagc ttgcactaac
    10441 atcagtgaca gtaaatgcta tcgcacctgg atttgtagcc ggtaatatgg caagtgagtg
    10501 gcaagaagat gaacttcaag caatgataac tgaattacca caacagcgat tggttttacc
    10561 gagtgaggtt gctcatacat gcgcctattt atatcactca aatgctagaa gcgttactgg
    10621 aactatacag aaagttaatg gtgcttggta tatttaaaat atagaactat ttttatgagc
    10681 aaatgttttt atgattgatg ttataatatg aatattacaa tgaatcataa tttgttaatt
    10741 aaacaagggg tgaggttcac catgacagtt gcagagaaaa aagaatggta cctagaatac
    10801 gaaattgaaa ttaatagacc gggtctttta ggtgatgtat ctagtttatt aggtatgtta
    10861 ggtataagta ttgttacaat taatggtgtt gatcaaggta aacgaggcct tttaattaaa
    10921 acagacaatc ttgaaaaagt tgaacgtttt gagcaaatag ctcgtggtat aaatgaaatt
    10981 gaaataacaa agcttaaaaa accagaatta agagaccgtc ttgcagttag acatggtaga
    11041 tatattgagc aagatgcaaa agataagaaa acctttcgat ttgagcgtga agatttaggc
    11101 ttgttagtag actttttagc tgaattgttc aaagaagaag gtcataagtt gattggcatt
    11161 agaggtatgc cacgggttgg taaaactgaa tcaattgttg cgggaagtgt ttgtgcacat
    11221 aagagatggt tatttattag ttctacttta ataaaacaaa ctgtacgtag ctctctaatt
    11281 aaaggggaat atgatgccaa tcatgtatac attattgatg gtgcagttac tgccagagaa
    11341 tctaatccaa aacatcaaga gcttgttaac gaagttatga cgttaccatc aatcaaagtc
    11401 gttgaacatc cagatttatt tgttgaaaca agtacttgta caatggaaga ttttgactat
    11461 attatcgaat tgagagaaaa tgaaaatcaa gaaatacatt acgaagaaat gaagaaacag
    11521 acagtccaaa gtaagaataa tttagatttt ggagatccgt ttggtggtgg ttttggtttc
    11581 ttcgagtaag tgtaaggagg ctatgaattg aaaacggtcg gtgaagcgct aaaaggtaga
    11641 cgtgaaaggt taggaatgac tttaacagaa ttagagcaac gtactggaat taaacgtgaa
    11701 atgctagtgc atattgaaaa taatgaattc gatcaactac cgaataaaaa ttacagcgaa
    11761 ggatttatta gaaaatatgc aagcgtagta aatattgaac ctaaccaatt aattcaagct
    11821 catcaagatg aaattccatc gaaccaagcc gaatgggacg aagtaattac agttttcaat
    11881 aataataaag acttagatta taagagtaaa tcaaaagagc c
//
LOCUS       DAOBBH010000070        11826 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.070, whole genome shotgun sequence.
ACCESSION   DAOBBH010000070 DAOBBH010000000
VERSION     DAOBBH010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11826
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.070"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            56..358
                     /locus_tag="P6L01_001380"
     CDS             56..358
                     /locus_tag="P6L01_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030454.1"
                     /translation="MQIEKLRGAALDELFDAILTLENREECYQFFDDLCTVNEIQSLS
                     QRLQVAKMIKQGYTYATIEQESGASTATISRVKRSLQWGNDAYTMILDRMNIETNE"
     gene            530..1222
                     /locus_tag="P6L01_001381"
     CDS             530..1222
                     /locus_tag="P6L01_001381"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HDP3030455.1"
                     /translation="MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVT
                     EDNVIHLMSRIRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVAFHNGTLLEALK
                     TYGHSIDFEEVIFEGYVVCNADSKVAKHTKANTDLTTEDLEAYAQMVNHMYRLPVMYI
                     EYSGIYGDVSKVQAVSEHLTETQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKK
                     ALKTVKIKESSK"
     gene            1219..3411
                     /gene="pcrA"
                     /locus_tag="P6L01_001382"
     CDS             1219..3411
                     /gene="pcrA"
                     /locus_tag="P6L01_001382"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDP3030456.1"
                     /translation="MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     LDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADR
                     IGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
                     ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHV
                     DEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTI
                     FLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIR
                     EIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLS
                     YLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSK
                     KVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENID
                     EFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIV
                     FIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQS
                     NMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVSSSDWNVGD
                     KVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED"
     gene            3415..5418
                     /gene="ligA"
                     /locus_tag="P6L01_001383"
     CDS             3415..5418
                     /gene="ligA"
                     /locus_tag="P6L01_001383"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDP3030457.1"
                     /translation="MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIK
                     IEEEHPEYKTVDSPTVRVGGEAQASFKKVNHDTPMLSLGNAFNEDDLRKFDQRIREQI
                     GNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
                     NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFI
                     YSVNDFTDFNARSQSEALDELDKLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYD
                     IDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTA
                     ILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDA
                     VTYHMPTHCPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKI
                     IQQLYQSELIKDVADIFYLTEEDLLPLDRMGQKKVDNLLAAIQQAKDNSLENLLFGLG
                     IRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENEDIRALIQ
                     KLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSS
                     VTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVDKQNELNS"
     gene            5431..6630
                     /locus_tag="P6L01_001384"
     CDS             5431..6630
                     /locus_tag="P6L01_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HDP3030458.1"
                     /translation="MKRTLVLLITAIFILAACGNHKDDQAGKDNQKHNNSSNQVKEIA
                     TDKNVQGDNYRTLLPFKESQARGLLQDNMANSYNGGDFEDGLLNLSKEVFPTDKYLYQ
                     DGQFLDKKTINAYLNPKYTKREIDKMSEKDKKDKKANENLGLNPSHEGETNPEKIAEK
                     SPAYLSNILEQDFYGGGDTKGKNIKGMTIGLAMNSVYYYKKEKDGPTFSKKLDDSEVK
                     KQGKQMASEILSRLRENDDLKDIPIHFAIYKQSSEDSITPGEFITQATAEKSQTKLNE
                     WHNINEKSALLPSSTAADYDENLNNNFKQFNDNLQSYFSNFTQAVGKVKFVDKKPQRL
                     VVDLPIDYYGQAETIGITQYVTEQANKYFDKIDNYEIRIKDGNQPRALISKTKDDKEP
                     QVHIYSN"
     gene            complement(6719..8257)
                     /gene="putP"
                     /locus_tag="P6L01_001385"
     CDS             complement(6719..8257)
                     /gene="putP"
                     /locus_tag="P6L01_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDP3030459.1"
                     /translation="MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNL
                     SEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSAMWITIGLTLGAYINY
                     FVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
                     GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAM
                     MNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIVRFMSIKSHK
                     MLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVG
                     GFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVV
                     AIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALV
                     VIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDI
                     VREK"
     gene            8624..8926
                     /gene="gatC"
                     /locus_tag="P6L01_001386"
     CDS             8624..8926
                     /gene="gatC"
                     /locus_tag="P6L01_001386"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDP3030460.1"
                     /translation="MTKVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDS
                     ADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTIMNEEDA"
     gene            8928..10385
                     /gene="gatA"
                     /locus_tag="P6L01_001387"
     CDS             8928..10385
                     /gene="gatA"
                     /locus_tag="P6L01_001387"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDP3030461.1"
                     /translation="MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFL
                     ALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPI
                     YESTVMEKLHKENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
                     AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTR
                     NVKDNAIVLEAISGADVNDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDV
                     KEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSK
                     EAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKV
                     FENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRP
                     IGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL"
     gene            10398..11825
                     /gene="gatB"
                     /locus_tag="P6L01_001388"
     CDS             10398..11825
                     /gene="gatB"
                     /locus_tag="P6L01_001388"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDP3030462.1"
                     /translation="MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAY
                     PGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGEN
                     GYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
                     KEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFN
                     YVRKGLEYEEKRQEEELLNGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIV
                     PLYIDDAWKERVRQTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHG
                     ADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPE
                     LAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQ
                     IMKASKGQANPQLVNQLLKQELDKR"
BASE COUNT         4437 a         1756 c         2205 g         3428 t
ORIGIN      
        1 atagattaat gcgttaaaat gacataaaga ataaaattat aggggttgtt tcgatatgca
       61 aattgaaaaa ttacgaggtg cagcgttaga tgaattgttt gatgcaatac taacgttaga
      121 aaatagagaa gaatgttacc aatttttcga tgatttgtgt actgtaaatg aaattcaatc
      181 actgtctcaa agattacaag ttgctaaaat gattaagcaa ggttatacct atgcaacgat
      241 tgaacaagaa tctggagcat cgactgcaac gatttctaga gtgaaacgtt cattacaatg
      301 gggtaatgat gcttatacaa tgattttaga tcgtatgaat attgaaacaa atgaataata
      361 aatgaacgat aaacaatggt tatctatctg cactaataag gtagataatc attgtttttt
      421 cacgaaaaaa tttacagagt aaaagaactt aaaattcata ttaagtcttt agaactcgac
      481 acttaaaaat gctataatca tatgtatgtt aaaaaaggag tttcggaaaa tgtatgacat
      541 taaaaaatgg cgccatattt ttaaattaga cccagctaaa catatttcag atgatgattt
      601 agatgcgatt tgtatgtctc aaacagatgc aattatgatt ggtggaactg atgacgttac
      661 tgaagataat gtcattcatt taatgagcag aataagaaga tacccattgc ctttagtact
      721 tgaaatatca aatatcgaaa gtgtaatgcc tggttttgat ttttattttg tacctacagt
      781 actgaacagt acagatgttg catttcacaa tggtacatta ttagaagcgc ttaaaacata
      841 tggacatagt atagattttg aagaagtaat atttgaaggg tatgtcgtgt gcaatgctga
      901 tagcaaagtg gcaaaacata ccaaagcaaa tacagattta acaacagaag atttagaagc
      961 atatgcccaa atggtcaatc atatgtatcg attaccggtt atgtatatag agtatagtgg
     1021 catttatggc gacgtatcaa aggttcaagc tgtctcagaa catctaacag aaacgcaact
     1081 tttttatggt ggcggtattt cctcagaaca acaagcgaca gagatggcag ctattgcaga
     1141 tacaattatc gtcggtgata ttatttataa agatattaaa aaagctttaa aaacagtaaa
     1201 aataaaggag tctagtaaat gaatgcgtta ttaaatcata tgaatacaga gcaaagtgaa
     1261 gctgtaaaga caacagaagg accattgtta attatggcag gtgctggttc agggaagaca
     1321 cgtgttttaa cacatagaat tgcttattta ttagacgaaa aagatgtctc accatacaat
     1381 gttttggcta ttacttttac aaataaagct gcaagagaaa tgaaagaacg tgttcaaaaa
     1441 ttagtaggtg atcaagcaga agttatttgg atgtcaacat tccactcaat gtgtgttcgt
     1501 attttacgtc gtgatgcaga tcgaataggt atagaacgca attttacgat aattgatcct
     1561 acagaccaaa aatctgttat taaagacgtc ttaaaaaatg aaaatattga tagtaaaaag
     1621 tttgaacctc gtatgtttat cggtgcgatc agtaatttga aaaatgaact taaaacacct
     1681 gcagatgctc aaaaagaagc cacagattat cactcgcaaa tggtagcaac ggtttatagt
     1741 ggatatcaac gccaattgtc gcgtaatgaa gcgttagatt ttgatgacct tattatgaca
     1801 acgattaact tatttgagcg tgtaccagaa gttctagaat attatcagaa caaattccaa
     1861 tatattcatg tagatgagta tcaagatact aataaagcac aatatacatt agttaaatta
     1921 ttagcaagta agtttaaaaa cttatgtgtt gtaggtgact cagatcagtc aatttatggt
     1981 tggcgtggtg ctgatattca aaatatctta tcatttgaaa aagactaccc agaagcgaat
     2041 acaatctttt tagagcaaaa ttatcgttcg acgaaaacga ttttaaatgc ggctaacgaa
     2101 gtgattaaaa ataattctga acgtaagcca aaaggactgt ggactgcaaa tacgaatggt
     2161 gagaaaattc attactatga agcaatgaca gaacgtgatg aagcggaatt tgtaatacga
     2221 gaaattatga agcatcaacg taatggtaag aaatatcaag atatggcaat tttatataga
     2281 acaaatgcac aatcacgtgt acttgaggaa acattcatga aatctaatat gccatacaca
     2341 atggttggtg gccaaaagtt ctatgaccgt aaagaaatca aagatttatt aagttatttg
     2401 cgtataattg ccaatagtaa tgatgacatt agtttgcaac gtattattaa tgttccaaaa
     2461 agaggtgtag gtccttcatc tgttgaaaaa gttcaaaact atgcacttca aaacaatatc
     2521 agtatgtttg atgcacttgg agaagctgat tttatcggat tgtcaaaaaa ggtgacacaa
     2581 gagtgtctta atttttacga gttaatccaa agcttgataa aagaacaaga atttttagaa
     2641 attcatgaaa tagttgatga agtattacaa aagtctggct accgagaaat gcttgaacgt
     2701 gaaaatacac tagaatctcg aagtagatta gaaaacatcg atgaatttat gtcagtacca
     2761 aaagactatg aggaaaatac cccattagaa gaacagtcat taattaactt tttaacggat
     2821 ttatcgttag tagctgatat tgatgaggca gatactgaaa atggcgtaac actaatgacg
     2881 atgcactcgg ctaagggcct tgaatttcca atcgtcttta taatggggat ggaagaatct
     2941 ttattcccac atattagagc gattaagagt gaagatgatc acgaaatgca agaagaacgt
     3001 cgtatttgtt atgtagcaat tacaagggct gaagaggtgt tatatatcac tcatgcgaca
     3061 tcaagaatgt tatttggtcg ccctcagtcg aatatgccat ctagattttt aaaggaaatt
     3121 ccagaatcac tattagaaaa tcattcaagt ggcaaacgac aaacgataca acctaaggca
     3181 aaaccttttg ctaaacgcgg atttagtcaa cgaacaacgt caacgaaaaa acaagtatcg
     3241 tcatctgatt ggaatgtagg tgacaaagtg atgcataaag cctggggaga aggcatggtg
     3301 agtaatgtaa acgagaaaaa tggctcaatc gaactagata ttatctttaa atcacaaggg
     3361 ccaaaacgtt tgttagcgca atttgcacca attgaaaaaa aggaggatta agggatggct
     3421 gatttatcgt ctcgtgtgaa cgagttacat gatttattaa atcaatacag ttatgaatac
     3481 tatgtagagg ataatccatc tgtaccagat agtgaatatg acaaattact tcatgaactg
     3541 attaaaatag aagaggagca tcctgagtat aagactgtag attctccaac agttagagtt
     3601 ggcggtgaag cccaagcctc tttcaaaaaa gtcaaccatg acacgccaat gttaagttta
     3661 gggaatgcat ttaatgagga tgatttgaga aaattcgacc aacgcatacg tgaacaaatt
     3721 ggcaacgttg aatatatgtg cgaattaaaa attgatggct tagcagtatc attgaaatat
     3781 gttgatggat acttcgttca aggtttaaca cgtggtgatg gaacaacagg tgaagatatt
     3841 accgaaaatt taaaaacaat tcatgcgata cctttgaaaa tgaaagaacc attaaatgta
     3901 gaagttcgtg gtgaagcata tatgccgaga cgttcatttt tacgattaaa tgaagaaaaa
     3961 gaaaaaaatg atgagcagtt atttgcaaat ccaagaaacg ctgctgcggg atcattaaga
     4021 cagttagatt ctaaattaac ggcaaaacga aagctaagcg tatttatata tagtgtcaat
     4081 gatttcactg atttcaatgc gcgttcgcaa agtgaagcat tagatgagtt agataaatta
     4141 ggttttacaa cgaataaaaa tagagcgcgt gtaaataata tcgatggtgt tttagagtat
     4201 attgaaaaat ggacaagcca aagagagtca ttaccttatg atattgatgg gattgttatt
     4261 aaggttaatg atttagatca acaggatgag atgggattca cacaaaaatc tcctagatgg
     4321 gccattgctt ataaatttcc agctgaggaa gtagtaacta aattattaga tattgaatta
     4381 agtattggac gaacaggtgt agtcacacct actgctattt tagaaccagt aaaagtagct
     4441 ggtacaactg tatcaagagc atctttgcac aatgaggatt taattcatga cagagatatt
     4501 cgaattggtg atagtgttgt agtgaaaaaa gcaggtgaca tcatacctga agttgtacgt
     4561 agtattccag aacgtagacc tgaggatgct gtcacatatc atatgccaac ccattgtcca
     4621 agttgtggac atgaattagt acgtattgaa ggcgaagtag cacttcgttg cattaatcca
     4681 aaatgccaag cacaacttgt tgaaggatta attcactttg tatcaagaca agccatgaat
     4741 attgatggtt taggcactaa aattattcaa cagctttatc aaagcgaatt aattaaagat
     4801 gttgctgata ttttctattt aacagaagaa gatttattac ctttagatag aatggggcag
     4861 aaaaaagttg ataatttatt agctgccatt caacaagcta aggacaactc tttagaaaat
     4921 ttattatttg gtctaggtat taggcattta ggtgttaaag cgagccaagt gttagcagaa
     4981 aaatatgaaa cgatagatcg attactaacg gtaactgaag cggaattagt agaaattcat
     5041 gatataggtg ataaagtagc acaatctgta gttacttatt tagaaaatga agatattcgt
     5101 gctttaattc aaaaattaaa agataaacat gttaatatga tttataaagg tatcaaaaca
     5161 tcagatattg aaggacatcc tgaatttagt ggtaaaacga tagtactgac tggtaagcta
     5221 catcaaatga cacgcaatga agcatctaaa tggcttgcat cacaaggtgc taaagttaca
     5281 agtagcgtta ctaaaaatac agatgtcgtt attgctggtg aagatgcagg ttcaaaatta
     5341 acaaaagcac aaagtttagg tattgaaatt tggacagagc aacaatttgt agataagcaa
     5401 aatgaattaa atagttagag gggtatgtcg atgaagcgta cattagtatt attgattaca
     5461 gctatcttta tactcgctgc ttgtggtaac cataaggatg accaggctgg aaaagataat
     5521 caaaaacata acaatagttc aaatcaagta aaagaaattg ctacggataa aaatgtacaa
     5581 ggtgataact atcgtacatt gttaccattt aaagaaagcc aggcaagagg acttttacaa
     5641 gataacatgg caaatagtta taatggcggc gactttgaag atggtttatt gaacttaagt
     5701 aaagaagtat ttccaacaga taaatatttg tatcaagatg gtcaattttt ggacaagaaa
     5761 acaattaatg cctatttaaa tcctaagtat acaaaacgtg aaatcgataa gatgtctgaa
     5821 aaagataaaa aagacaagaa agcgaatgaa aatttaggac ttaatccatc acacgaaggt
     5881 gaaacaaatc ctgaaaagat tgcagaaaaa tcaccagcct atttatctaa cattttagag
     5941 caagattttt atggtggtgg agatacaaaa ggtaagaata ttaaaggtat gacgattggt
     6001 ttagctatga atagtgttta ttactataaa aaagaaaaag atggaccgac ttttagtaaa
     6061 aaactagatg atagcgaagt taaaaagcaa ggtaaacaaa tggctagtga gatattatca
     6121 aggttacgtg aaaatgatga tttaaaagat ataccaattc attttgcaat ttataagcaa
     6181 tcaagtgaag attcaatcac accaggtgaa tttatcactc aagcgactgc agaaaagagt
     6241 caaacaaagc ttaatgaatg gcataatatc aatgaaaaat cagctttatt accttcttca
     6301 acagcagcag attatgatga aaatttaaat aataatttca agcaatttaa tgataatttg
     6361 caatcatatt tttctaattt cacacaagca gtagggaaag ttaaatttgt tgataaaaag
     6421 ccacaacgat tagtagtaga tttaccaatc gattactatg gacaagctga aacaattggt
     6481 attacacagt acgttactga acaagcgaat aaatatttcg ataaaatcga taactatgaa
     6541 attcgtatta aagatggtaa ccaaccacgt gctttaatta gtaagacaaa agatgacaaa
     6601 gaaccgcaag ttcatattta cagtaattaa ttgtaaatta aagataataa aaaggtacca
     6661 atgttagtca taattttgat taacattagt acctttttaa tttgttaaga cttatgaatt
     6721 atttttctct aacgatgtca cgaactttgt ttaagtcagt ttcaacaaat gcaccaggtt
     6781 ttttagtaag tttacttaca acatatgtaa cgattacact tacaataaat ccaggaataa
     6841 tttcatataa gccgaatatt tcgttgatat gtgccaatgg tttaatccac gcaatccaaa
     6901 cgataacgac taaggcacct gaaaccattc cacttacagc accggcacgt gtcaaacctt
     6961 tccagtaaag tgcaaatagc acaagtggac tgaacgatgc accaaatccg gcccaagcgt
     7021 tacctactaa gtttagaatt gtgtcgtttg gattccatgc aatcgcgatg gcaacaattg
     7081 ctacaactaa tacagataat cttccaatca taacaaattc tttttggtgc gttttagctt
     7141 tttcttcacc acgaattaat ttataaaagt cttccgttag tgaactagat gttacaagta
     7201 attgtgaaga aatcgtgctc ataattgcag ctagaatcgc agcaagtaag aaaccaccta
     7261 caagaggatg gaagagtact tgactcatca cgatgaataa tgtctcagga tcttctagtt
     7321 taatatgata agcaggtacg aatgcaatac ctgttaaacc aacagccaca gcgcctaata
     7381 aaccaacagc catccagcta atacctaaac gtctagcttt aggtagcatc ttgtgtgatt
     7441 taatagacat aaaccttaca atgatatgag gttgaccgaa ataacctaat ccccatgaaa
     7501 atagagagat aattcctata aatgataacc ctttaaataa atttaaattt gtaggtttca
     7561 tagctgctac atcatgaaac gttccccagc cgtttaaatt catcatagca acaataggga
     7621 ccataaccat cgcaattaac ataatgacac cttggaagaa atctgtaatt gatacagcta
     7681 aatatccacc aaagaaagta tagaaaatga caatgaaagc aactaatatt aaaccgaaat
     7741 gataatctaa tccaaaagca ctttcaaata atttaccacc agatacgaaa ccagaatgtg
     7801 tatataatgt aaagaatact acgataatca atccagaaat aatctttaac acattatttt
     7861 tatcgtttaa acgattttta aagaaatctg gtaatgtaat tgcatctcca gctaattcgg
     7921 tataaacacg aagtctagga gcaacaacaa agtaatttat ataagcacct aatgttaaac
     7981 cgattgtaat ccacatagct gatagaccag tgctatagac agaaccaggt agccccataa
     8041 tcatccatcc actcatatct gaagctccag ctgataatgc agtaatatac ggtccaatac
     8101 tacgtccacc taacatgtac tcgcttaggt taccagttgc ttgcttgtaa ccgtaaaagc
     8161 caataacgat tagtatcaag aagtagacgg caatcataat ataagtttgc caattggcat
     8221 ctacttgttg acttaatgct gtccccattg taagcatgat aaacactccc tttatttaat
     8281 ttataattta gttacttgaa aacattatac ataaagataa atgtttagaa aagtaggaaa
     8341 attaaattta ttctttttga aatacttgca taccaatata ggaagcgctt tcgtattttt
     8401 tggatatgag caaattttta taattaaaat tgcataatca taatattgaa ttgggttgtc
     8461 taatattaaa agagtgttgt tttcataaaa aatgatttga taaacgaatg ataatgttta
     8521 tacagtttat atgaaattaa agtagcacct cataaatact tagattttta attggaaatt
     8581 tgatacaatt tagtgatgaa tgacttaaag gaggctttta ttaatgacaa aagtaacacg
     8641 tgaagaagtt gagcatatcg cgaatcttgc aagacttcaa atttctcctg aagaaacgga
     8701 agaaatggcc aacacattag aaagcatttt agattttgca aaacaaaatg atagcgctga
     8761 tacagaaggc gttgaaccta catatcacgt tttagattta caaaacgttt tacgtgaaga
     8821 taaagcaatt aaaggtattc cgcaagaatt agctttgaaa aatgccaaag aaacagaaga
     8881 tggacaattt aaagtgccta caatcatgaa tgaggaggac gcgtaagatg agcattcgct
     8941 acgaatcggt tgagaattta ttaactttaa taaaagacaa aaaaatcaaa ccatctgatg
     9001 ttgttaaaga tatatatgat gcaattgaag agactgatcc aacaattaag tcttttctag
     9061 cgctggataa agaaaatgca atcaaaaaag cgcaagaatt ggatgaatta caagcaaaag
     9121 atcaaatgga tggcaaatta tttggtattc caatgggtat aaaagataac attattacaa
     9181 acggattaga aacaacatgt gcaagtaaaa tgttagaagg ttttgtgcca atttacgaat
     9241 ctactgtaat ggaaaaacta cataaagaga atgccgtttt aatcggtaaa ttaaatatgg
     9301 atgagtttgc aatgggtggt tcaacagaaa catcttattt caaaaaaaca gttaacccat
     9361 ttgaccataa agcagtacca ggtggttcat caggtggatc tgcagcagca gttgcagctg
     9421 gcttagtacc atttagctta ggttcagaca caggtggttc aattagacaa ccggctgcat
     9481 attgtggcgt tgtcggtatg aaaccaacat acggtcgtgt atctcgattt ggattagttg
     9541 cttttgcatc ttcattagac caaattggtc cattgactcg aaatgtaaaa gataatgcaa
     9601 tcgtattaga agctatttct ggtgcagatg ttaatgactc tacaagtgca ccagttgatg
     9661 atgtagactt tacatctgaa attggtaaag atattaaagg attaaaagtt gcattaccta
     9721 aagaatactt aggtgaaggt gtagctgatg acgtaaaaga agcagttcaa aacgctgtag
     9781 aaactttaaa atctttaggt gctgtcgttg aggaagtatc attgccaaat actaaatttg
     9841 gtattccatc atattacgtg attgcatcat cagaagcttc gtcaaacctt tctcgttttg
     9901 acggaattcg ttatggttat cattctaaag aagctcattc attagaagaa ttatataaaa
     9961 tgtcaagatc tgaaggtttc ggtaaagaag taaaacgtcg tattttctta ggtacatttg
    10021 cattaagttc aggttactac gatgcttact ataaaaaatc tcaaaaagtt agaacattga
    10081 ttaaaaatga ctttgataaa gtattcgaaa attatgatgt agtagttggt ccaacagcgc
    10141 ctacaactgc gtttaattta ggtgaagaaa ttgatgatcc attaacaatg tatgccaatg
    10201 atttattaac aacaccagta aacttagctg gattacctgg tatttctgtt ccttgtggac
    10261 aatcaaatgg ccgaccaatc ggtttacagt tcattggtaa accattcgat gaaaaaacgt
    10321 tatatcgtgt cgcttatcaa tatgaaacac aatacaattt acatgacgtt tatgaaaaat
    10381 tataaggagt ggaaatcatg cattttgaaa cagttatagg acttgaagtt cacgtagagt
    10441 taaaaacgga ctcaaaaatg ttttctccat caccagcgca ttttggagca gaacctaact
    10501 caaatacaaa tgttatcgac ttagcatatc caggtgtctt accagttgtt aataagcgtg
    10561 cagtagactg ggcaatgcgt gctgcaatgg cactaaatat ggaaatcgca acagaatcta
    10621 agtttgaccg taagaactat ttctatccag ataatccaaa agcatatcaa atttctcaat
    10681 ttgatcaacc aattggtgaa aatggatata tcgatatcga agtcgacggt gaaacaaaac
    10741 gaatcggtat tactcgtctt cacatggaag aagatgctgg taagtcaaca cataaaggtg
    10801 agtattcatt agttgacttg aaccgtcaag gtacaccgct aattgaaatc gtatctgaac
    10861 cagatattcg ttcacctaaa gaagcatatg catatttaga aaaattacgt tcaattattc
    10921 aatacactgg tgtatcagac gttaagatgg aagagggatc tttacgttgt gatgctaaca
    10981 tctctttgcg tccatatggt caagaaaaat ttggtactaa agccgaattg aaaaacttaa
    11041 actcatttaa ctatgtacgt aaaggtttag aatatgaaga aaaacgccaa gaagaagaat
    11101 tgttaaatgg tggagaaatc ggacaagaaa cacgtcgatt tgatgaatct acaggtaaaa
    11161 caattttaat gcgtgttaaa gaaggttctg atgattaccg ttacttccca gagcctgaca
    11221 ttgtaccttt atatattgat gatgcttgga aagagcgtgt tcgtcagaca attcctgaat
    11281 taccagatga gcgtaaggct aagtatgtaa atgaattagg tttacctgca tacgatgcac
    11341 acgtattaac attgactaaa gaaatgtcag atttctttga atcaacaatt gaacacggtg
    11401 cagatgttaa attaacatct aactggttaa tgggtggcgt aaacgaatat ttaaataaaa
    11461 atcaagtaga attattagat actaaattaa caccagaaaa tttagcaggt atgattaaac
    11521 ttatcgaaga cggaacaatg agcagtaaaa ttgcgaagaa agtcttccca gagttagcag
    11581 ctaaaggtgg taatgctaaa cagattatgg aagataatgg cttagttcaa atttctgatg
    11641 aagcaacact tctaaaattt gtaaatgaag cattagacaa taacgaacaa tcagttgaag
    11701 attacaaaaa tggtaaaggc aaagctatgg gcttcttagt tggtcaaatt atgaaagcgt
    11761 ctaaaggtca agctaatcca caattagtaa atcaactatt aaaacaagaa ttagataaaa
    11821 gataat
//
LOCUS       DAOBBH010000071        11708 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.071, whole genome shotgun sequence.
ACCESSION   DAOBBH010000071 DAOBBH010000000
VERSION     DAOBBH010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11708
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.071"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            61..2193
                     /locus_tag="P6L01_001389"
     CDS             61..2193
                     /locus_tag="P6L01_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDP3030463.1"
                     /translation="MLLSTREKEMIALLIKYHSQYITIHDIAQQLAVSSRTIHRELKG
                     VEAYLTSFSLTLERANKKGLRIAGADSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALI
                     QAKEPVKQYSLAQEIGVSVQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKR
                     EFLSQLMVNNLNSTSVYSVIENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPY
                     QLTESSYLTLTVHIVLSISRIKNGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYD
                     VTFNQAEIAFITIHLRGAKRKNLNDTSLNNRCEENKIKAFVNKVEMISGMTFADLDTL
                     VDGLTLHLNPAINRLQANIETYNPLTDMIKFKYPRLFENVRLALNDCWPDLIFPESEI
                     AFIVLHFGGSIKNQGNRFLNILVVCSSGMGTSRLLSTRLEQVFSEIERITQASVSDLK
                     SLDLSQYDGIISTVNLDIDSPYLTVNPLLPDSDISYVAQFLNTKSTFQETHDKSSNMI
                     DKDDVHVETKDVDGNTSFENEQTSYLTSVFEKHLSDEKSEQLLHHMRSGLTLLDSVKI
                     VSTEVKQWQTYIADYLYQCDVINDPTSFAELLEQRLIDNPGWILSPYPVAIPHLRDNM
                     IKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFIPDNDFMASLVSDLSEFLSLKLES
                     IDTFMENPQELETLLRNKFLERIKKQFI"
     gene            2205..2639
                     /locus_tag="P6L01_001390"
     CDS             2205..2639
                     /locus_tag="P6L01_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDP3030464.1"
                     /translation="MSELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAM
                     KDREQVVSTFMGNGLAIPHGTDEAKTNVIHSGLTLLQIPEGVDWDGEVVKVVVGIAGK
                     DGEHLDLLSKIAITFSEEENVDRIVQAKSAEEIKQVFEEADA"
     gene            2639..3745
                     /locus_tag="P6L01_001391"
     CDS             2639..3745
                     /locus_tag="P6L01_001391"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDP3030465.1"
                     /translation="MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALAHDH
                     QYDVILADESKTTTRVNNVDAINSMQPSEALKQAILEADIITTAVGVNILPIIAKSFA
                     PFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNEN
                     ILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFA
                     GKATVLDAVKDSSIEAGLRRVLAETSQYITNEFDFTEAEQAGYVEKIIDRFNNSYLSD
                     EVTRVGRGTLRKIGPKDRIIKPLTYLYNKDLERTGLLNTAALLLKYDDTADQETVEKN
                     NYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLS"
     gene            complement(4064..11509)
                     /locus_tag="P6L01_001392"
     CDS             complement(4064..11509)
                     /locus_tag="P6L01_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HDP3030466.1"
                     /translation="MNLFRQQKFSIRKFNVGIFSALIATVTFISTNPTTASAAEQNQP
                     AQNQPAQPADANTQPNANAGAQANPAAQPANQGGQANPAGGAAQPAGQGNQADPNNAA
                     QAQPGNQAAPANQAGQGNNQATPNNNATPANQTQPANAPAAAQPAAPVAANAQTQDPN
                     ASNTGEGSINTTLTFDDPAISTDENRQDPTVTVTDKVNGYSLINNGKIGFVNSELRRS
                     DMFDKNNPQNYQAKGNVAALGRVNANDSTDHGNFNGITKTVNVKPDSELIINFTTMQT
                     NSKQGTTNLVIKDAKKNTELATVNVAKTGTAHLFKVPTDADRLDLQFIPDNTAVADAS
                     RITTNKDGYKYYSFIDNVGLFSGSHLYVKNRDLAPKATNNKEYTINTEIGNNGNFGAS
                     LKADQFKYEVTLPQGVTYVNDSLTTTFPNGNEDSTVLKNMTVNYDQTANKVTFTSQGV
                     TTARGTHTKEVLFPDKSLKLSYKVNVANIDTPKNIDFNEKLTYRTASDVVINNAQPEV
                     TLTADPFSVAVEMNKDALQQQVNSQVDNSHYTTASIAEYNKLKQQADTILNEDANHVE
                     TANRASQADIDGLVTKLQAALIDNQAAIAELDTKAQEKVTAAQQSKKVTQDEVAALVT
                     KINNDKNNAIAEINKQTTSQGVTTEKDNGIAVLEQDVITPTVKPQAKQDIIQAVTTRK
                     QQIKKSNASLQDEKDVANDKIGKIETKAIKDIDAATTNAQVEAIKTKAINDINQTTPA
                     TTAKAAALEEFDEVVQAQIDQAPLNPDTTNEEVAEAIERINAAKVSGVKAIEATTTAQ
                     DLERVKNEEIFKIENITDSTQTKMDAYKEVRQAATARKAQNATVSNATDEEVAEANAA
                     VDAAQTEGLHDIQVVKSQQEVADTKAKVLDKINAIQTQAKVKPAADTEVENAYNTRKQ
                     EIQNSNASTTEEKEAAYTELDAKKQEARTNLDAANTNSDVTTAKDNGIAAINQVQAAT
                     TKKSDAKAEIAQKASERKTAIEAMNDSTTEEQQAAKDKVDQAVVTANADIDNATANTD
                     VDNAKTTNEATIAAITPDANVKPAAKQAIADKVQAQETAIDANNGSTTEEKEAAKQQV
                     QTEKTAADAAIDAAHSNVEVEAAKNAEIAKIEAIQPATTTKDNAKQAIATKANERKTA
                     IAQTQDITAEEIAAANADVDNAVTQANSNIEAANSQNDVDQAKTTGETSIDQVTPTVN
                     KKATARNEITAILNNKLQEIQATPDATDEEKQAADAEANTENGKANQAISAATTNAQV
                     DEAKANAEAAINAVTPKVVKKQAAKDEIDQLQATQTNVINNDQNATNEEKEAAIQQLA
                     TAVTDAKNNITAATDDNGVDTAKDAGKNSIQSTQPATAVKSNAKNEVDQAVTTQNQAI
                     DNTTGATTEEKNAAKDLVLKAKEKAYQDILNAQTTNDVTQIKDQAVADIQGITADTTI
                     KDVAKDELATKANEQKALIAQTADATTEEKEQANQQVDAQLTQGNQNIENAQSIDDVN
                     TAKDNAIQAIDPIQASTDVKTNARAELLTEMQNKITEILNNNETTNEEKGNDIGPVRA
                     AYEEGLNNINAATTTGDVTTAKDTAVQKVQQLHANPVKKPAGKKELDQAAADKKTQIE
                     QTPNASQQEINDAKQEVDTELNQAKTNVDQSSTNEYVDNAVKEGKAKINAVKTFSEYK
                     KDALAKIEDAYNAKVNEADNSNASTSSEIAEAKQKLAELKQTADQNVNQATSKDDIEV
                     QIHNDLDNINDYTIPTGKKESATTDLYAYADQKKNNISADTNATQDEKQQAIKQVDQN
                     VQTALENINNGVDNGDVDDALTQGKAAIDTIQVDATVKPKANQAIEAKAEDTKESIDH
                     SDQLTAEEKTEALAMIKQITDQAKQGITDATTTDEVEKAKAQGLEAFDNIQIDSTEKQ
                     KAIEELETALDQIEAGVNVDADATTEEKEAFTNALEDILSKATEDISDQTTNAEIATV
                     KNSALEQLKAQRINPVVKKNALEAIREVVNKQIEIIKNADADASAKEIARTDLGRYFD
                     RFADKLDKTQTNTEVAELQNVTIPAIEAIVPQNDPDANDTNNGTDNNDATANSNANAT
                     PENTGQPNVSETTDNGKADASPTTPNNSDAATGETTVTSATDDAKDKPQANNNSSADA
                     STNSPTMDNDVTSKPEVESTNNGTTDKPVTETDNATPAESTTNNNSTTTATNENAPTG
                     STATAPTTASTEAASSADSKDNASVNDSKQNAEVNNSAESQSTNGKVAQPKSENKAKA
                     EKDGRDSTNQSMVESTTETLPSADITEPNVPSNTSKDKEESTTNQTDAGQLKSETNVA
                     SNEADKSPSKADTEVSNKPSTSASSEAKDKMTSTNVSQKDDTATADTNDTQKSVGPVA
                     NNKAKDMQTNDTQKSVGSAANNKATQNDGANASPATVSNGSHSMHQDMLNVTKPEENK
                     ANAKSDQQGKVNKPKQQAKTLPDTGMSHNDDLPYAELALGAGMAFLIRRFTKKDQQTE
                     E"
BASE COUNT         3127 a         1966 c         2051 g         4564 t
ORIGIN      
        1 atctaaaaaa agatgatcaa gcataataat taaataaatt aaaaaatgga ggataccgcc
       61 atgttattga gtacacgtga aaaagaaatg atagccctat tgattaagta ccacagtcaa
      121 tatatcacta tacacgacat tgctcagcaa cttgcggtgt cctctcgtac tattcaccgt
      181 gaattaaaag gtgttgaagc atatttaact tcattttcat taactttaga acgcgcaaac
      241 aaaaaagggc tacgcattgc tggcgcagat tctgatttaa acgatttgaa gcaatcgatt
      301 gcacaacatc aaaccattga cttatctgtt gaagagcaga aagtaattat tatatacgct
      361 ttgatacaag ccaaggagcc agttaaacaa tatagtttag cgcaagaaat tggcgtttct
      421 gtccagactt tagcaaagat gttagatgat ttagagcttg atttaaataa gtaccaacta
      481 tctttatctc gaaagcgtgg cgaaggcatt tacttggtag gtactgaatc aaagaaacgt
      541 gaatttttaa gtcaattaat ggtgaataac ttaaatagta ctagcgttta ttcagtaatt
      601 gaaaatcatt ttgtctttca ttcattaaat caaatccaca aagactttgt tgacttagag
      661 cgcattttta atgttgaaag actattaatg gactacctaa gtgccttacc ctaccaactt
      721 accgaatcaa gttatttaac tttaactgtc catatcgtgc tatccatttc acgtataaaa
      781 aatggagagt atgtcgcatt aaacgatgat atttatgatt ctgtacaaaa cacatttgaa
      841 cacaaagtag caagcgaact tgctgataaa cttggtcaaa tatatgacgt cacgtttaat
      901 caggcagaaa ttgctttcat tactatccat ttacgtggag ctaaacgaaa aaatcttaat
      961 gatacatcat taaataatcg ttgtgaagaa aacaaaatta aagcgtttgt taacaaagta
     1021 gaaatgattt ccggtatgac atttgcagat ttggatactt tagtagatgg actgacgcta
     1081 caccttaatc ctgcaatcaa tcgtttgcaa gctaatatcg agacctataa tccgttaaca
     1141 gacatgatta agttcaaata tccaagacta tttgaaaatg taagattagc tttaaatgat
     1201 tgttggcctg atttgatttt tccagagagt gaaattgctt ttatagtttt acactttggt
     1261 ggctcgatta aaaaccaagg taatcgattt ttaaacatat tagtcgtttg cagcagtggt
     1321 atgggaacta gtcgtctatt atcaactcgt ctagagcaag tttttagtga gattgagcgt
     1381 attacacaag catcagtcag cgatttgaag tcactagatt taagtcaata tgatggcatt
     1441 atttctactg tgaatttaga catcgactcc ccctatttaa cggtaaaccc attattacca
     1501 gatagtgata tcagttatgt cgcacagttt ttaaatacaa agtctacgtt ccaagagacg
     1561 catgataaat catcaaacat gattgataag gatgatgttc atgttgaaac gaaagatgtt
     1621 gatggcaaca catcttttga aaatgaacaa acttcatact taacttcagt tttcgaaaaa
     1681 catttaagtg acgaaaaatc agaacaatta ttgcatcata tgcgttcggg tttaactttg
     1741 cttgattcag tgaaaatagt tagtaccgaa gttaaacagt ggcaaacata tatcgcagat
     1801 tatctatatc aatgcgatgt aataaacgat ccaacgtcat tcgctgaact actagagcaa
     1861 cgattgattg acaatccagg atggatatta agtccatatc ctgttgcaat accacaccta
     1921 agagacaata tgattaaaca ccctatgatt ctaatcacag ttttagaaga accgttaaca
     1981 ttgcctagta ttcaaaatga caatcaaaca attaaatata tgatttccat gtttattcct
     2041 gacaatgatt ttatggcatc actggtaagt gacttgtccg aatttttaag tttgaaatta
     2101 gaatctattg atacttttat ggaaaatcca caggaacttg aaacattatt aagaaacaaa
     2161 tttttagaac gaattaaaaa acaatttatt taggagtgta caaaatgagc gaattattta
     2221 gtaatgacaa tatcttttta aatgtaaatg ttaacagcca aaatgaagca attgaaaaag
     2281 caggtaaagc cttagttgat agtggtgctg taacagatgc ttatattcaa gcaatgaaag
     2341 atcgtgagca agttgtatca acatttatgg gaaatggctt agcaattcct catggcacag
     2401 atgaagctaa aacaaatgtg attcactcag gtttaacatt attacaaatc cctgaaggcg
     2461 ttgactggga tggcgaagta gttaaagttg tagtgggaat cgctggtaaa gatggcgaac
     2521 atttagactt gttatctaaa attgcaatta catttagcga agaagaaaat gtggatcgta
     2581 tcgttcaagc aaaatctgca gaagaaatta aacaagtatt cgaggaggca gatgcataat
     2641 gaaagcagtt cactttggtg ctggtaacat aggtcgtggt ttcattggtt atattcttgc
     2701 agacaacaat gttaaagtaa catttgcaga cgtcaatgaa gaaatcatta atgctttagc
     2761 tcatgatcat caatacgatg ttattttagc tgatgagtct aaaacaacga cgcgcgtgaa
     2821 taatgttgat gcaattaatt caatgcaacc ttctgaagcg ttgaaacaag caattctaga
     2881 agctgatatt attacaacag ctgttggtgt taacatacta cctattattg ctaaatcttt
     2941 tgcgcctttc ttaaaagaaa aaacaaacca tgttaatatt gttgcttgtg agaatgctat
     3001 tatggcaact gatacattga aaaaagcagt acttgatatt actggccctc ttggtaacaa
     3061 tattcatttt gctaactcag cagttgatag aattgtacca ttacaaaaga atgaaaatat
     3121 attagacgtt atggttgagc cattttacga atgggttgtt gaaaaagatg catggtatgg
     3181 tccagaacta aaccatatta aatatgttga tgatttaaca ccatatattg agcgtaaatt
     3241 attaactgtg aatacaggac atgcatattt agcgtatgct ggtaaatttg caggtaaagc
     3301 taccgtatta gacgctgtaa aagatagttc aattgaagct ggcttacgcc gtgttttagc
     3361 tgaaacaagt caatatatta ctaatgaatt tgattttact gaagcggaac aagctggtta
     3421 tgttgaaaaa ataatagacc gtttcaacaa ttcttattta tctgatgaag tgacacgtgt
     3481 cggacgaggt acgttacgta aaattggccc taaagataga attataaaac cattaacata
     3541 tctttataat aaagatttag aacgcactgg tttattaaat acagctgcat tgttattgaa
     3601 gtatgatgat acagcagacc aagaaactgt tgagaaaaat aattacatta aagaacacgg
     3661 tttaaaagcg tttttaagcg aatatgctaa agttgacgat ggcttagccg atgaaataat
     3721 tgaagcgtac aattcacttt cataatttat tgagctttgt ttgaaacaag aagtttccaa
     3781 cgttattcgt taacaatcag taataatgta gtagttccgt tgaattaaca atattaaatt
     3841 tctgaacata aaaaatactc ccttcaacat agacacttaa cttgtgttat gtatgaaagg
     3901 agtatttttg cgttactaat ttgttttatt ttcgagccac agcgacctat tcaattgcta
     3961 ttggtcatta ctaaaacaaa ttcatattaa ctgttagact tggttactta gtaaggaata
     4021 tttccctatg aaataactag atgttcacat tcttgaataa attttattct tcagtttgtt
     4081 ggtctttctt agtgaatctt ctaattaaga atgccatacc tgcacctaga gctaattcag
     4141 catatggtaa atcgtcatta tgtgacatac cagtatctgg taaagtttta gcttgttgtt
     4201 taggcttatt aacttttcct tgttggtctg attttgcatt tgctttgttt tcttcaggct
     4261 tagttacatt aagcatatct tgatgcatac tatgactacc atttgaaact gttgctggag
     4321 atgcattggc accgtcgttt tgcgtagctt tattgtttgc agctgaacca actgattttt
     4381 gcgtatcatt agtttgcata tctttagctt tattgtttgc aactgggcca actgattttt
     4441 gcgtatcatt agtatctgct gttgccgtat catctttttg gctaacatta gttgaagtca
     4501 ttttatcttt tgcttcagaa gatgcagatg ttgatggttt attcgaaact tcagtatcag
     4561 ctttgcttgg cgatttatct gcttcgttag atgcaacgtt agtttcagac ttaagttgtc
     4621 ctgcatcagt ttgatttgtc gtactttctt ctttatcttt tgatgtatta gaaggtacat
     4681 ttggttctgt tatgtctgct gaaggcaatg tttcagttgt tgattcaacc atactttgat
     4741 ttgttgaatc tctaccatct ttttctgcct tagctttatt ttcagatttt ggttgtgcaa
     4801 ccttgccatt agttgattga gattcagcac tattatttac ttcagcattt tgttttgaat
     4861 catttacaga tgcattatct ttactatcag cagatgatgc tgcttctgtg ctcgcagttg
     4921 ttggagccgt tgctgttgat cctgttggtg cattctcgtt tgttgctgta gttgtactat
     4981 tgttatttgt tgtgctttct gctggcgttg cattatctgt ttctgttaca ggtttatcag
     5041 ttgtgccgtt attagttgat tctacttctg gtttactagt tacatcgtta tccattgtcg
     5101 gactgtttgt tgatgcatct gcactagaat tgttattagc ttgcggttta tcctttgcat
     5161 catcagttgc tgatgttact gttgtttcac ctgttgccgc atcactatta tttggtgttg
     5221 tcggagaagc gtctgctttg ccattatctg tcgtctcaga tacgttaggt tgtccagtgt
     5281 tttctggtgt tgcattagca tttgaatttg ctgttgcatc attattatct gtaccattat
     5341 tagtatcatt agcatctgga tcattctgag gcacaatcgc ttcaattgca ggtatcgtta
     5401 cattttgtaa ttcagcaacc tctgtatttg tttgtgtttt atctaattta tcagcaaatc
     5461 tgtcaaaata tctacctaaa tctgtacgtg caatttcttt cgccgatgca tctgcatctg
     5521 catttttaat tatttctatt tgcttgttaa ccacttctct aattgcttcc aaagcatttt
     5581 tcttaactac tggattaata cgttgtgctt taagttgttc aagcgcacta tttttgacag
     5641 tagcgatttc tgcatttgta gtttgatcag aaatatcttc agttgctttt gataaaatgt
     5701 cttctaaagc attcgtaaac gcttcttttt cttcagttgt agcatcagcg tcgacattta
     5761 cacctgcttc aatctggtct agtgcagttt ctaattcttc gatagctttt tgtttttctg
     5821 ttgagtcaat ttgaatgtta tcaaatgcct caagtccttg agctttcgct ttttcaactt
     5881 catcagttgt tgttgcatca gtaatacctt gtttagcttg atctgtaatt tgtttaatca
     5941 ttgctaatgc ttcagttttt tcttcagcag ttaactggtc actatgatca attgattctt
     6001 tcgtatcttc tgctttagct tcaatagctt ggttcgcttt aggtttaaca gtagcatcta
     6061 cttgaatagt atcaattgct gctttacctt gtgttaatgc atcatcaacg tcaccattat
     6121 ccacaccatt attaatgttt tctaatgcag tttgaacatt ttggtcaact tgcttaattg
     6181 cttgttgctt ttcatcttgt gttgcattag tgtcagctga aatattattt ttcttctgat
     6241 ctgcataagc atataaatct gttgtagctg attctttttt acctgttgga attgtgtaat
     6301 cgttaatatt atctaagtca ttatgaattt gaacttcaat gtcatcttta gaagtagctt
     6361 gattaacatt ttgatccgca gtttgtttta attcagcaag tttttgtttc gcttcagcaa
     6421 tttcacttga agtcgatgcg ttagagttat ccgcttcgtt tactttagca ttatatgcat
     6481 cttcaatttt agctaaagca tcttttttgt actcactaaa tgttttaact gcattaattt
     6541 tagcttttcc ttctttaact gcattatcaa catattcatt tgttgatgat tgatcgacat
     6601 ttgttttcgc ttgatttaat tcagtatcaa cttcttgttt tgcatcatta atttcttgtt
     6661 gtgatgcatt tggtgtttgt tctatttgtg ttttcttatc agctgcagct tgatctaatt
     6721 cttttttacc tgctggtttc ttaacaggat ttgcatgaag ttgttgaact ttttgtactg
     6781 ctgtatcttt agcagtagtt acatcacctg tagtagttgc tgcattaata ttatttaaac
     6841 cttcttcata tgctgctcta actggtccaa tatcgttacc tttttcttca ttagtagtct
     6901 cattattatt aagtatttca gttattttat tttgcatttc agttagcaat tctgctcttg
     6961 cattcgtttt aacatctgtt gatgcttgaa ttgggtcaat tgcttgaatt gcattatctt
     7021 ttgcagtgtt tacatcatcg attgactgtg cattttcaat attttgatta ccttgtgtta
     7081 attgtgcgtc tacttgttga tttgcttgtt ctttttcttc agtagtcgca tctgcagttt
     7141 gtgcaataag cgctttttgt tcgtttgctt ttgttgctaa ttcatctttc gcaacatctt
     7201 taattgttgt atctgcagta ataccttgaa tatcagcaac tgcttgatct ttaatttgcg
     7261 taacatcatt agttgtttgt gcatttaaga tatcttgata cgctttttct ttagctttta
     7321 aaactaaatc ttttgctgca tttttctctt cagttgtagc gccagttgta ttatcaattg
     7381 cttgattttg agttgtcaca gcttgatcaa cttcattttt cgcattcgat ttaactgctg
     7441 ttgctggttg tgtgctttga attgaattct ttccagcgtc tttcgctgta tctacaccat
     7501 tatcatcagt tgcagctgta atattatttt tcgcgtctgt aactgctgtt gctaattgtt
     7561 gaatagctgc ttctttttct tcatttgtag cgttctgatc attattgata acatttgttt
     7621 gcgttgcttg taattgatca atctcatctt tagccgcttg tttcttaaca acttttggtg
     7681 ttaccgcatt aattgctgct tctgcattag ctttcgcttc atcaacttgt gcgttagtag
     7741 ttgctgctga aatggcttga tttgctttac cattttcagt atttgcttct gcatcagctg
     7801 cttgtttttc ttcatctgtt gcatctggtg tagcttgaat ctcttgcaat ttgttattta
     7861 aaattgctgt gatttcatta cgtgcagttg cttttttatt aactgttggt gttacttgat
     7921 caatactagt ttcacctgtc gttttcgctt ggtctacatc attttgacta ttagcagctt
     7981 caatgttgct atttgcttgt gtcacagcat tatctacgtc cgcattagcc gctgcaattt
     8041 cttcagcagt aatatcttgc gtttgagcga ttgctgtttt acgttcattc gctttcgtag
     8101 caattgcttg tttcgcatta tctttagttg ttgttgctgg ctgaatcgct tcaattttag
     8161 caatttctgc atttttagcc gcttcaactt ccacatttga atgagctgca tctattgctg
     8221 catcagcagc cgttttttca gtttgaactt gttgtttagc agcttctttt tcttctgttg
     8281 ttgaaccgtt attagcatca attgctgttt cttgagcttg tactttatct gcaattgctt
     8341 gttttgctgc tggtttaaca tttgcatctg gcgtaattgc agctattgta gcttcattag
     8401 tagtttttgc attatctaca tctgtatttg ctgtagcatt atctatatca gcgtttgcag
     8461 taactactgc ttgatcaact ttatcttttg cagcttgttg ttcttcagta gtcgaatcat
     8521 tcattgcttc aattgcagtt ttacgttcac ttgctttttg agcgatttcc gctttagcat
     8581 ccgatttctt agttgtggca gcttgcactt gattaattgc agcaatacca ttgtctttag
     8641 ctgttgttac atcactgttt gtatttgcag catcaagatt tgttcttgct tcttgctttt
     8701 tagcatctaa ttctgtatat gcagcttctt tttcttctgt agttgaagca ttgctatttt
     8761 ggatttcttg tttacgtgta ttatatgcgt tttctacttc tgtatcagct gcaggtttaa
     8821 cttttgcttg tgtttgaatt gcattgattt tatctaatac ttttgcttta gtatcagcca
     8881 cttcttgttg tgatttaaca acttggatgt catgtaaacc ttctgtttga gctgcatcta
     8941 ctgctgcatt tgcctctgct acttcttcat cagttgcatt tgaaactgta gcattttgcg
     9001 cttttctagc tgtagctgct tgtctaactt ctttataagc atccatttta gtttgagttg
     9061 aatccgtaat attctcaatt ttgaatattt cttcgttttt aacgcgttct aaatcttgtg
     9121 cagtcgttgt cgcttcaatt gctttaacac cagaaacttt agctgcatta atacgttcaa
     9181 tagcttccgc tacttcttca tttgttgtat caggatttaa aggtgcttga tcaatttgtg
     9241 cttgaacaac ttcgtcaaat tcttcaagag ctgctgcttt agctgttgta gcaggtgtag
     9301 tttgattaat atcattgatt gcttttgttt taatggcttc tacttgtgca tttgttgttg
     9361 ctgcatcaat atctttaatt gcctttgttt caattttacc aattttatca tttgctacat
     9421 ctttttcatc ttgtaatgat gcatttgact ttttaatttg ttgtttacga gttgtaactg
     9481 cttggataat atcttgtttc gcttgaggtt taactgttgg tgtaatcaca tcttgttcta
     9541 acactgcgat accattatct ttttcagttg tgacaccttg tgatgttgtt tgtttattaa
     9601 tttctgcgat tgcattattt ttatcattgt taattttagt cacaagcgct gcaacttcat
     9661 cttgcgtaac ttttttactt tgttgtgctg ctgtaacctt ttcttgagct ttagtatcta
     9721 attcagcaat tgctgcttga ttatcaatta atgcagcttg taatttagtt actaaaccgt
     9781 caatatccgc ttgagatgca cgatttgcag tttcaacgtg attcgcatct tcatttaaaa
     9841 tagtgtctgc ttgttgttta agtttattgt attctgcaat tgatgctgtt gtgtaatgac
     9901 tattatcaac ttgtgagttt acttgttgtt gcaacgcatc tttgttcatt tcaaccgcta
     9961 ctgaaaatgg atctgcagtt agtgtaactt ctggttgcgc attattaatt acaacatctg
    10021 aagcagtacg atatgttaat ttttcattaa aatcaatatt tttaggtgta tcgatattcg
    10081 caacattaac tttatatgat aattttaaag atttatctgg gaataaaact tctttagtgt
    10141 gtgtaccacg tgccgttgtc acaccttggc ttgtaaatgt aactttattt gcagtttgat
    10201 cataattaac agtcatattt ttcaatactg tactgtcttc attaccatta gggaatgttg
    10261 tagttaatga atcattaacg taagttacac cttgtggtaa tgttacttca tatttaaatt
    10321 gatctgcttt taatgaagca ccaaaattac cattgttacc gatttcagta ttaatagtat
    10381 attctttatt gttagttgct ttcggtgcta aatctctatt tttgacatat aaatgtgatc
    10441 ctgagaatag acctacatta tcaatgaatg aatagtattt ataaccatct ttatttgttg
    10501 taattcttga agcatcagca actgctgtat tgtcaggaat aaattgtaaa tctaaacgat
    10561 cagcatcagt tggtacttta aataaatgtg cagtaccagt cttagcaaca tttacagttg
    10621 ctaattcagt atttttctta gcatctttaa taactaaatt tgttgtacct tgcttactat
    10681 tcgtttgcat agtagtaaag ttaataatta attctgaatc tggttttaca tttacagttt
    10741 ttgtaatacc gttaaagtta ccatgatctg tagaatcatt tgcattcaca cgacctaatg
    10801 cagccacgtt tcctttagct tgatagtttt gagggttatt cttatcaaac atatcgcttc
    10861 gtcttaattc tgagttaacg aaaccaatct taccgttgtt aattaatgaa taaccattta
    10921 ctttatctgt aacagttaca gttggatcct gtctattctc atctgttgat atggcaggat
    10981 catcaaatgt taatgtcgta ttaatactgc cttcaccagt attgctagca tttggatctt
    11041 gagtttgtgc gtttgctgct acaggtgctg ctggttgcgc tgctgctgga gcattcgctg
    11101 gctgtgtttg atttgccggt gttgcattat tattaggtgt tgcttggtta tttccttgac
    11161 ctgcttggtt tgccggtgct gcttgatttc caggttgtgc ttgtgcagcg ttattcggat
    11221 cagcttgatt accttgtcca gctggttgtg ctgctcctcc tgctggatta gcctgtccac
    11281 cttggtttgc tggctgtgct gcaggattag cttgagcacc agcatttgcg ttaggctgtg
    11341 tattggcatc agctggttgt gctggttgat tttgtgcagg ctgattttgc tctgctgcag
    11401 acgctgttgt cgggttagta gatataaaag taacagtggc aattaaagct gaaaaaatac
    11461 cgacattaaa ttttctgata ctaaattttt gttgtctgaa taaattcatt aagtcatcct
    11521 cctggttgat tattctcgct gttaaatgat ttcacttaat caactgttaa gataagtagt
    11581 agcatctgcg ttaaaaacac aaagcaactc tatctaatta aaattaattt tatcatcatt
    11641 atatattgag taccagtgta ttttatatta catattgatt actttgtttt tattttgttt
    11701 atatcatt
//
LOCUS       DAOBBH010000072        11605 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.072, whole genome shotgun sequence.
ACCESSION   DAOBBH010000072 DAOBBH010000000
VERSION     DAOBBH010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11605
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.072"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..736)
                     /locus_tag="P6L01_001393"
     CDS             complement(<1..736)
                     /locus_tag="P6L01_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDP3030467.1"
                     /translation="MKPLHLKLNNFGPFLKEEIDFSKIDNNELFLISGKTGSGKTMIF
                     DAMTYALFGKASTEQREENDLRSHFADGKQPMSVTFEFQLNHRIYKVHRQGPYIKEGN
                     TTKTNAKFDVFEMVDGKYEIRESKVISGTQFIIELLGVNADQFRQLFILPQGEFKRFL
                     ISNSREKQGILRTLFDSEKFEAIREILKEELKKEKAQIENRYQQIDLLWQEIESFDDG
                     KIKGLLELATQQIDKLIENIPLLQARS"
     gene            complement(740..1861)
                     /locus_tag="P6L01_001394"
     CDS             complement(740..1861)
                     /locus_tag="P6L01_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDP3030468.1"
                     /translation="MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIV
                     IAGDLYDTTYPSKDAIMLLEQAIGKLNLELRIPIIMISGNHDGKERLNYGASWFEHNQ
                     LFIRTDFTSINSPIEINGVNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPE
                     IDEDAVNILISHLTVQGGKTSDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIE
                     DDKIKYSGSLLQYSFSEAGQAKGYRRLTINDGIINDVFIPLKPLRQLEIISGEYNDVI
                     NEKVHVKNKDNYLHFKLKNMSHITDPMMSLKQIYPNTLALTNETFNYNEENNAIEISE
                     KDDMSIIEMFYKHITDKELSDIQSKKIKNILENELRKED"
     gene            complement(1985..2452)
                     /locus_tag="P6L01_001395"
     CDS             complement(1985..2452)
                     /locus_tag="P6L01_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdC family protein"
                     /protein_id="HDP3030469.1"
                     /translation="MLYLIFSIIVALFMGTIVIVIRMKAQNYPVNEKKIVLPPFFMAT
                     GALMYVVPYFRLTGSEMLEAFIIGLLFSTVLIWTSRFEVKGTEIYMKRSKAFPVILIS
                     LLIIRTVMKIFISNEIDPGELGGMFFLLAFCMIVPWRAAMLYKYKKLKKTLIN"
     gene            complement(2546..2644)
                     /locus_tag="P6L01_001396"
     CDS             complement(2546..2644)
                     /locus_tag="P6L01_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030470.1"
                     /translation="MRKSKFAIDRGYFPDMEMASFYNQINKNKYVY"
     gene            complement(2631..2873)
                     /locus_tag="P6L01_001397"
     CDS             complement(2631..2873)
                     /locus_tag="P6L01_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDP3030471.1"
                     /translation="MATWLAIIFIVAALILGLIGGFLLARKYMMDYLKKNPPINEEML
                     RMMMMQMGQKPSQKKINQMMTMMNKNMDQNMKSAKK"
     gene            complement(3151..5139)
                     /gene="tkt"
                     /locus_tag="P6L01_001398"
     CDS             complement(3151..5139)
                     /gene="tkt"
                     /locus_tag="P6L01_001398"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDP3030472.1"
                     /translation="MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWT
                     RHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHP
                     EYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLM
                     EGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKD
                     GNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEVERKLTFEN
                     YGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISG
                     KLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDAT
                     DYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIM
                     GLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALES
                     ESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVE
                     AAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVG
                     TAGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL"
     gene            complement(5260..5499)
                     /locus_tag="P6L01_001399"
     CDS             complement(5260..5499)
                     /locus_tag="P6L01_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDP3030473.1"
                     /translation="MSNSDLNIERINELAKKKKEVGLTQEEAKEQTALRKAYLESFRK
                     GFKQQIENTKVIDPEGNDVTPEKIKEIQQKRDNKN"
     gene            complement(5636..5869)
                     /locus_tag="P6L01_001400"
     CDS             complement(5636..5869)
                     /locus_tag="P6L01_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030474.1"
                     /translation="MFYNKYKNVSTYIIIFLVSSAAFAIFLLSANVSAHSEQVYEMTD
                     HQIKNNTINKAYEHKDPTNNGEQRDGKVFALIN"
     gene            6012..6635
                     /gene="lexA"
                     /locus_tag="P6L01_001401"
     CDS             6012..6635
                     /gene="lexA"
                     /locus_tag="P6L01_001401"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDP3030475.1"
                     /translation="MRELTKRQSEIYNYIKQVVQMKGYPPSVREIGEAVGLASSSTVH
                     GHLSRLEEKGYIRRDPTKPRAIEIVSDQTNDNINMEETIHVPVIGKVTAGVPITAVEN
                     IEEYFPLPEHLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMT
                     EEDEATVKRFYKEKNRYRLQPENSTMEPIYLDNVAVIGKVIGLYREM"
     gene            complement(7015..8031)
                     /locus_tag="P6L01_001402"
     CDS             complement(7015..8031)
                     /locus_tag="P6L01_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HDP3030476.1"
                     /translation="MKKFIVTVVAFLSIIIIAPITEFKPFIHLQNEVRQYIDIHINKE
                     TISAENKLDTPKKQQFAFNNIQMNMSKSDVEKTLNKPKRVTFNEYGTKWYTYYDDDYN
                     NFIMISYMKDKVNALYTNQNIITSKSKIKYNTPKSVVRQRLGEPETEIVKGRVRYEQN
                     NKEYDVFHKNHIYTTVFYDKHRRNNVTAVLQVSDAMENRLKEQYGAPSKSLADSFELQ
                     NFDLVNAERKQHQLSTLKYSKQNSETARKHSKDMAKNHYFDHTNLKGQSPFDRLKKDG
                     ITFNSAGENLAYGQVSSIYAHQGLMNSIGHRKNILNDTFKILGVGVDFNDEKQPFWTE
                     NYTG"
     gene            complement(8053..9030)
                     /gene="guaC"
                     /locus_tag="P6L01_001403"
     CDS             complement(8053..9030)
                     /gene="guaC"
                     /locus_tag="P6L01_001403"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDP3030477.1"
                     /translation="MKIFDYEDIQLIPNKCIVESRSECDTTIQFGPKKFKLPVVPANM
                     QTVMNEKLAKWFAENDYFYIMHRFDEEARIPFIKHMQNSGLFASISVGVKKAEFDFIE
                     KLAQEKLIPEYITIDIAHGHSDSVINMIKHIKNHIPDSFVIAGNVGTPEGVRELENAG
                     ADATKVGIGPGRVCITKIKTGFGTGGWQLAALNICSKAARKPLIADGGIRTHGDIAKS
                     IRFGASMVMIGSLFAAHEESPGETVELDGKQYKEYFGSASEFQKGEHKNVEGKKMFVE
                     HKGSLMDTLKEMQQDLQSSISYAGGKDLKSLRTVDYVIVRNSIFNGDRD"
     gene            complement(9187..9456)
                     /gene="rpsN"
                     /locus_tag="P6L01_001404"
     CDS             complement(9187..9456)
                     /gene="rpsN"
                     /locus_tag="P6L01_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDP3030478.1"
                     /translation="MAKKSKIAKERKREELVNKYYELRKELKAKGDYEALRKLPRDSS
                     PTRLTRRCKVTGRPRGVLRKFEMSRIAFREHAHKGQIPGVKKSSW"
     gene            complement(9910..10059)
                     /gene="rpmG"
                     /locus_tag="P6L01_001405"
     CDS             complement(9910..10059)
                     /gene="rpmG"
                     /locus_tag="P6L01_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDP3030479.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERIEMKKYCPRLNKYTL
                     HRETK"
     gene            complement(10150..>11605)
                     /locus_tag="P6L01_001406"
     CDS             complement(10150..>11605)
                     /locus_tag="P6L01_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HDP3030480.1"
                     /translation="SMTAGPRGPLLMQDIYFLEQMSQFDREVIPERRMHAKGSGAFGT
                     FTVTKDITKYTNAKIFSEIGKQTEMFARFSTVAGERGAADAERDIRGFALKFYTEEGN
                     WDLVGNNTPVFFFRDPKLFVSLNRAVKRDPRTNMRDAQNNWDFWTGLPEALHQVTILM
                     SDRGIPKDLRHMHGFGSHTYSMYNDSGERVWVKFHFRTQQGIENLTDEEAAEIIATDR
                     DSSQRDLFEAIEKGDYPKWTMYIQVMTEEQAKNHKDNPFDLTKVWYHDEYPLIEVGEF
                     ELNRNPDNYFMDVEQAAFAPTNIIPGLDFSPDKMLQGRLFSYGDAQRYRLGVNHWQIP
                     VNQPKGVGIENICPFSRDGQMRVVDNNQGGGTHYYPNNHGKFDSQPEYKKPPFPTDGY
                     GYEYNQRQDDDNYFEQPGKLFRLQSEDAKERIFTNTANAMEGVTDDVKRRHIRHCYKA
                     DPEYGKGVAKALGIDINSIDLETENDETYENFEK"
BASE COUNT         3626 a         2127 c         1567 g         4285 t
ORIGIN      
        1 tcgacctagc ttgtaaaagt ggtatatttt caatcaattt gtctatctgc tgagtggcaa
       61 gctctaataa gccttttatt ttgccatcat caaatgattc aatttcttgc cataaaaggt
      121 caatttgttg atatctattc tcgatttgag ctttttcttt ttttagttct tcttttaata
      181 tttctcgtat agcttcaaat ttttcactgt caaacagtgt tcttaatatc ccttgctttt
      241 cacgactgtt tgatattaaa aagcgtttga attcaccttg aggcaaaata aacaattgtc
      301 ggaattgatc tgcatttact cctaataatt caataatgaa ttgggtacct gaaattactt
      361 tactttctct aatttcatac ttgccatcca ccatctcaaa tacatcaaat ttagcgttcg
      421 tttttgttgt attaccttct ttgatataag ggccttgtct atgcacttta taaattcgat
      481 gatttaattg aaattcaaat gttactgaca tcggctgttt accatcagcg aaatgacttc
      541 tcaaatcatt ttcttctctt tgttcagttg atgctttacc aaacaaggca taagtcattg
      601 catcaaaaat cattgtttta cccgatccag tcttaccact tattaaaaac aattcattat
      661 tatcaatttt agaaaaatca atttcttctt ttaaaaaggg gccgaaatta ttcaacttta
      721 aatgtaatgg tttcattcat taatcctcct ttctcaattc gttttctaaa atatttttta
      781 tctttttaga ttggatatcc gataattctt tatcagttat atgtttataa aacatttcga
      841 taattgacat gtcatctttt tcacttattt ctatagcatt attttcttca ttataattaa
      901 aagtttcatt cgtcagcgct aaagtattag gataaatttg ttttaaactc atcattggat
      961 cagtaatatg agacatattt ttaagtttaa aatgtaaata attatcttta tttttcacat
     1021 gaactttttc attaataaca tcattatatt cgcctgagat aatttccaat tgtctaagtg
     1081 gcttaagagg aataaataca tcgttaataa tgccatcatt aattgttaaa cgtctatacc
     1141 ctttagcttg acccgcttcc gaaaatgaat actgcaataa ggagccacta tatttaattt
     1201 tgtcgtcttc tatactaaat ggatgatgca agtgacctag catgacataa tcaaatatat
     1261 caaaaacacc tttctgaact gattcaaccg ttccaatagt taatggtctt tcagaatcag
     1321 atgtctttcc accttgaaca gtcagatgac taattaaaat attgacggca tcttcatcaa
     1381 tttccggtgc tattgtttca atacagcgcg taattccctg ttgatgtgtt tcaatggtgt
     1441 catcttcaaa gtagtgtttc atttcgctca cagtagcata agggagtgta taaaaattaa
     1501 ccccatttat ctctattggt gaattaatcg atgtaaaatc tgttcttata aataactgat
     1561 tatgttcaaa ccaactcgcc ccatagttta acctctcttt accatcgtga tttccactaa
     1621 tcataattat tggtatacgc agttctaaat ttaactttcc aatcgcttgt tctaataaca
     1681 tgattgcatc tttacttgga tatgttgtgt catataaatc tccagctatc acaatgatat
     1741 caggttcttc ttctttcatt ttttctacga acatatctaa aatatacgct tgatcttcta
     1801 aaagctgttt gccatttaat attttcccta agtgccagtc tgctgtatgt ataattttca
     1861 ttactgcctc catatagaac atttgttcgc ttttatttta atataatcat ttcacctttt
     1921 caagtaaaat taaaaccact cattgactga tttagaagtc gatcacaagt ggttttaaaa
     1981 gtaattaatt gattaatgtt ttctttagtt ttttgtattt atatagcatt gctgctctcc
     2041 aaggaacaat catacagaat gctaataaaa agaacatgcc gcctaattct ccaggatcta
     2101 tttcattact aatgaatatt ttcatcacag tacgaatgat aagtaatgaa atcaaaataa
     2161 ctggaaatgc tttagatcgt ttcatataaa tttctgtacc tttgacttca aatcgagaag
     2221 tccaaattag aactgtagaa aaaagcaaac caattataaa ggcttctagc atttccgatc
     2281 ctgttagcct aaaatatgga acgacgtaca tcaatgcacc ggtcgccata aaaaacggtg
     2341 gcaaaactat ttttttctca tttaccggat aattttgagc tttcatacga ataactataa
     2401 ctatagttcc cataaataaa gctacaatga ttgaaaatat taaatagagc acgcagacac
     2461 cttctcgtta aataaactct ctgtaaatca tacaaaatga attatagcat ttctcagtct
     2521 attcgtcatt ttgttaactt taattttaat aaacatattt gtttttatta atttgattat
     2581 aaaaagaggc catttccata tctggaaaat agcctctatc aattgcgaat ttactttttc
     2641 gcactcttca tattttgatc catattttta ttcatcatcg tcatcatttg attaattttc
     2701 ttctgagaag gtttttgacc catttgcatc atcatcatac gaagcatttc ttcgttgatt
     2761 ggtgggtttt tcttcaagta gtccatcata tattttctag ctaaaaggaa acctccaatt
     2821 aaacctaaaa ttaatgcagc tactataaaa ataattgcta accaagttgc cattgtttca
     2881 cccgctttct ttatcctact gaattttact aaattcatca cattatttca agaactagtt
     2941 tattcaagta ttttaatatc gatactaaaa ataatatcaa aaatcatgct gttataacag
     3001 agcttttaaa gattaatctg aaatagtata ctttcataac ttatcactta tgatgtagtt
     3061 taagtgctat actctaaatt tcaacacttt aaaagaagcc taagattatg catcctagac
     3121 ttctaaacat actcgcttta taaattattc ttataagctc ataacttggt ttaagatatt
     3181 ttctttagta aatccatatt tttcaactac taaatcgcca ggtgcacttg cgccaaagcc
     3241 gtcaatagca ataactttac ctgcagtacc tacatattta tgccatccaa gcggtgaagc
     3301 catttcaatc gcaacacgtt ttgttacgct tgatggaata actgattctt tatattcttc
     3361 agattgttgt tcaaatgcat tccagttagg cattgaaaca acacgcactg atttaccttg
     3421 tttttcaaga tctttagcag cttcaactgc aagactaact tctgaacctg aagctaataa
     3481 taggaattct ggtgtctctt cagagccata aactgtatag gcaccttttc gaacgccttc
     3541 ttcaactaca tcttctggta catctaaaac cggtaagttt tgacgtgtca atactaatga
     3601 agtaggtgta gattcagatt ctaaggcaac ttcccatgct actcttgttt cattaccatc
     3661 agcaggacgg ataacattca tatttggaat agctcttaat ccagctaatt gttcaattgg
     3721 ttcatgagta ggaccatctt cacctactgc aattgaatca tgtgtgaaga tgaacgttgc
     3781 atttaatccc ataattgatg ataaacgtaa cgctggtttt aaataatcac taaatacgaa
     3841 gaatgttgca ccatatggat gtaaacctcc atgtgcagcc ataccattta cagcagcacc
     3901 catagcaaat tcacgtacac caaaccacac atttttacct tcaggtgttt cagaactata
     3961 atctgttgca tcatttacat tggatttgtt tgaaccagca aggtctgctg atccaccaaa
     4021 gaatgaaggg acagttttac tgattgcttg aataacagta ccagaatcag cacgagatgc
     4081 accattatga cccagttcaa aacgtggtaa ttcatcctta taatttttag gcaatttacc
     4141 actaatcgct aatttaaatt cttctgctaa ttcaggatat gtttctgcat atttttctaa
     4201 taatgaattc cattgagatt catcttcatt agcacgtttt aacatagtat tttggaaaat
     4261 ttcgtatacc tcttctgaaa cattaaaacg tttttcagga tctaaaccgt aattttcgaa
     4321 tgttaatttt ctttcaactt cacctaaagg tgccccatga acaccattag ttcctgcttt
     4381 attcggtgaa ccaaatccga ttgttgtttt aacttcaata atcgttggtc cttcttgaga
     4441 tttagctgta gtaatcgctt tatcaatttc ttctaaatca ttaccatctt taactagtaa
     4501 gtaattccaa ccatatgctt caaaacgagc ttttgtgttt tcagaaaaag ctttgtttaa
     4561 ttcgccatct aatgaaatat catttgaatc gtataaaaca actaatttac ttaatttatt
     4621 atgtccagca aatgaagctg cttcatgcga tataccttcc attaaatcac cgtcagaagc
     4681 taatacatat gtgtaatgat ctacaacatt atatccttct ttattaaatt tccctgctag
     4741 gtgatcttct gctaaagcta atcctactga catagcaaaa ccttgtccaa gtggtccagt
     4801 agtaacttct acaccatcag tatgtctgta ttcaggatgg cctggtgttt tagaacccca
     4861 ttgtctaaat tgctttaatt cttctaattc taaactacca gaaacatgta acaagctata
     4921 caataatgct gaaccatgcc ctgccgataa tacgaaacgg tctctattga agtaatcttt
     4981 agattgtgga ttaaaattca gatgacgtgt ccacaaagtg taagccattg gggcagctcc
     5041 cataggtaat cctggatgac cagaattcgc tttttcgatt gtgtcgatac ttagtgcacg
     5101 tagcgtatca acagctaatt gatctttttc attaaacatc ttgtgtatct tcccttcttc
     5161 aattttatca aatacattat aacttatttt aaaataaaaa gataagtaaa atacttattt
     5221 caagttataa tgtcagaaaa ttctttacag atttgtgatt taatttttat tatctctttt
     5281 ttgttgtatc tctttaattt tttcaggtgt tacatcatta ccttctggat caattacttt
     5341 agtattttca atttgttgtt taaacccttt tctaaaactc tcaagataag cttttcttaa
     5401 ggctgtttgc tcctttgctt cttcttgagt taatcctact tcttttttct ttttagctaa
     5461 ctcgttaatt ctttcgatat tcaaatcaga attactcaat taaaaaacct accttcaatt
     5521 tgtttattca atcattataa tactttaaca aaatataaca aatttaagac cagcaaaaaa
     5581 gtaataaaaa aacgctattt cgcaaaaata ggcaaaataa cgttgtgaca atgtatcaat
     5641 ttattaaagc gaacactttc ccatctcttt gttcgccatt gtttgtaggg tctttatgtt
     5701 cgtatgcttt atttatcgta ttgttcttaa tttgatggtc agtcatttcg tacacttgtt
     5761 ccgagtgagc actaacgttc gcacttaaca agaatattgc aaaggctgca cttgaaacta
     5821 aaaatatgat gatatatgtt gatacgtttt tatatttatt gtaaaacatc atttccactc
     5881 ctagaacatt tgtttgtata agtaatttac cagaacttat gttctgtgtc aatattttaa
     5941 cgaacaaatg tttgttttgg ttgtgaatta atgatatact gtatttaaat taatttaggg
     6001 agtgcctata tatgagagaa ttaacaaaac gacaaagcga aatatataac tatattaaac
     6061 aagttgttca aatgaaaggt tatccgccta gtgttcgcga aattggtgaa gcagttggct
     6121 tagcatccag ttcaactgtt catggtcatc tttcacgtct tgaagaaaaa ggatatataa
     6181 gaagagatcc aacgaaacca cgtgctatag aaattgtaag tgatcaaaca aatgataata
     6241 ttaatatgga agaaacgatt catgtgccag ttattggtaa agtcacagca ggtgttccta
     6301 ttaccgctgt agaaaatatt gaagagtatt ttccattacc tgaacactta acatcgacac
     6361 ataatagcga catattcata ttaaacgtcg taggcgacag tatgattgag gctggtatat
     6421 tagacggaga caaagtaatt gttcgcagtc aaaccatagc agaaaatgga gacattattg
     6481 ttgctatgac tgaggaagat gaagcaactg tcaaacgctt ctataaagaa aaaaatcgtt
     6541 atcgattaca acctgaaaat agtacaatgg agccaattta cctcgacaat gttgctgtaa
     6601 ttgggaaagt aattggtttg taccgcgaaa tgtaatattt taaaccgtta tatattatcg
     6661 taattgttaa accctcattt ttataaattt tggacctctt gaaaaagttt cgttttcaag
     6721 aggttttatt tattctaatc taaattcagt tcaaacagaa attgcgaatt gttagataat
     6781 ctcattcttt aatataaatt ttgaattaca gattcctgca aaatgttaca caaattaaaa
     6841 ctcgcccccg taataattta caggagcgag ccattattat ttttttcttt cttttttatt
     6901 aagtatatct ttgacgcgtt caagtatatc cgcatctttt ggtttatctg ttgtcatcat
     6961 gtttatctac ttccttacag acaaacatta ccctttaaac tcataccaaa aacattaacc
     7021 agtataattt tctgtccaaa aaggttgttt ttcatcatta aaatcaacac caacacctaa
     7081 tattttaaac gtatcattta aaatattttt tctgtgacca atagaattca ttaatccttg
     7141 atgtgcatag atactactaa cttgaccata tgctaaattc tctccggctg agttaaatgt
     7201 aataccatct tttttcaatc gatcaaatgg tgattgacct tttaaatttg tatgatcaaa
     7261 ataatgattt ttggccatat ctttactatg cttacgagca gtttcagaat tctgtttaga
     7321 atacttcaat gtagataatt gatgttgttt tctttcagca ttaactaaat caaaattttg
     7381 tagttcaaaa ctatctgcaa gcgatttcga aggtgctcca tattgttctt ttaatctatt
     7441 ttccatagca tcacttactt gtaaaacagc tgttacatta ttacgtcgat gcttatcata
     7501 aaataccgtc gtataaatgt gatttttatg gaaaacatca tattctttat tattttgttc
     7561 gtaacgcact ctacctttaa caatctctgt ttctggttcg cctaatcttt gccttacaac
     7621 cgatttaggt gtattgtatt taatttttga ttttgaagtg attatatttt gatttgtata
     7681 taacgcatta actttatctt tcatgtaact tatcattata aaattattgt aatcgtcatc
     7741 ataatacgta taccacttcg taccatattc attaaatgtc actctttttg gtttatttaa
     7801 tgttttctca acatctgatt tcgacatgtt catttgtata ttattaaagg caaattgttg
     7861 tttcttcggt gtatccaatt tattttccgc agaaattgtt tctttattga tgtgaatgtc
     7921 aatatattgt cttacttcat tttgtaaatg aatgaatggt ttaaattctg ttattggcgc
     7981 aatgataata attgataaaa acgcaacaac agttactatg aattttttaa tttacatcac
     8041 ctactataaa tattaatctc tatcaccgtt gaaaatagag tttctaacaa taacataatc
     8101 tacagtacgt aatgatttca agtcttttcc accggcatat gaaattgagc tttgtaaatc
     8161 ttgttgcatt tcttttaagg tatccattaa tgaaccctta tgttctacaa acattttttt
     8221 accttctaca tttttatgtt cgcctttttg aaattcagat gcactaccaa aatattcttt
     8281 atactgttta ccatcaagtt ctacagtttc accaggtgat tcttcgtgtg ccgcaaataa
     8341 tgaaccaatc atgaccattg atgcaccaaa tctaattgat ttagcgatgt cgccatgcgt
     8401 tcttatacca ccatcggcaa tcaaaggttt acgagctgct ttactacata tgtttaatgc
     8461 cgctaactgc caaccaccag taccaaaacc tgttttaatc tttgtaatac aaactctacc
     8521 aggacctata ccgactttgg tagcatcagc accagcattt tctaattctc taacaccttc
     8581 tggcgtacca acattaccag caataacaaa actatcaggt atatggtttt ttatatgttt
     8641 aatcatgttt atcactgaat ctgagtgacc atgcgcaata tctattgtaa tatattcggg
     8701 gattaatttt tcttgagcta acttttcaat aaaatcaaat tccgctttct ttacaccaac
     8761 tgaaatagat gcaaataagc ctgaattttg catatgtttt ataaaaggta ttcttgcttc
     8821 ttcatcaaaa cgatgcatga tataaaagta atcattttca gcaaaccatt tcgctaattt
     8881 ctcattcata actgtttgca tatttgcagg aactacaggt agcttgaatt ttttcggacc
     8941 aaattggata gttgtatcac attcagacct actttcaact atgcatttat taggtattaa
     9001 ttgaatatct tcgtaatcaa atattttcac tttaaatagc ccctttaaaa actaattatt
     9061 tttaaatgca aattaaaaac cgaatgtttc cttattaata tgagaaaaca ttcagtttat
     9121 agcattcgta tatactgtac attgttttat tgatattgta aatgggctaa agtgcgaaat
     9181 ttgattttac caacttgatt ttttaacacc tggaatttgt cctttgtgcg catgttctct
     9241 aaacgcaata cgagacattt caaatttacg taatacacct ctaggtcttc cagttacttt
     9301 acatcttcta gttaaacgtg taggtgatga atctcttggt aattttctta acgcttcgta
     9361 atcacctttt gcttttaact ctttacgtaa ttcgtaatat ttatttacta actcttctct
     9421 ttttctctct tttgctattt tagatttctt agccatatat agttacctca ctttttatca
     9481 aatcgtaatt attacgattt atataataac acaatcctca cataattcaa cagtaaagtt
     9541 tcatttcttt gttaaatcat tttctaaacc atcatattta agattaaagt gtattatcga
     9601 acatttataa aacataccca gaattacttc gtacttatta aattccgctt tgaaatttta
     9661 aattctacta tattaaatac tctttcaagc attcgctaac aaagtgactt tataaaatca
     9721 gtaatctatt tccttctata aatattctca aattttaata aagtaacaac taatacaatc
     9781 atatattgtt ctaccgatga aactcaatcc attaatcact cttcacaaaa atttcataaa
     9841 aaagcgagct ccccaatata ataggtaagc ccgctttatt ttcaaatcgt cgtatttgaa
     9901 tgataagaat tacttagttt cacgatgtaa cgtatatttg tttaatcttg ggcaatattt
     9961 tttcatttca atacgctcag gattattacg tttattttta gtagtgatat agttacgatc
    10021 gccacattct gtgcatgcta atgttacgtt tacgcgcacg ttaaacaccc tctcttgtta
    10081 ggaatcttta cgatttatta tgatatcata aactgctcaa ctacgcaata tagaaactac
    10141 atatcaaatt tatttttcaa agttttcgta tgtttcatca ttttcagttt caagatcaat
    10201 agaatttata tcaataccta atgcttttgc aacaccttta ccatattctg ggtcagcttt
    10261 gtaacaatga cgaatatgac gtcgtttaac atcatccgtt acgccttcca ttgcatttgc
    10321 tgtatttgta aaaattcttt ctttagcgtc ctctgattgt aatctaaaca atttacctgg
    10381 ttgttcaaaa taattatcat catcttgacg ttgattatat tcatagccgt atccatcagt
    10441 tgggaatggt ggctttttat attcaggttg agaatcaaat ttaccatggt tatttggata
    10501 ataatgtgtt cctccacctt ggttattgtc aactacgcgc atttgaccat ctctactaaa
    10561 aggacaaata ttttcaatac caacaccttt aggttggttt acaggaatct gccaatgatt
    10621 aactcctaat cgatatcttt gcgcatcgcc atatgagaat aaacgccctt gcaacatttt
    10681 gtctggagaa aaatctaatc ctggaataat attagttggt gcaaacgcag cttgttcaac
    10741 atccataaag taattatctg gatttctatt taattcaaac tctccaactt caattagagg
    10801 atactcatcg tgataccata cttttgttaa atcaaatgga ttatctttat ggtttttagc
    10861 ttgttcctca gtcattactt gaatatacat tgtccatttt ggataatcac ctttttcaat
    10921 ggcttcgaat aaatcgcgtt gagatgaatc acgatctgta gctataattt cagcagcttc
    10981 ttcatcagtt aagttttcaa taccttgttg cgttctaaaa tggaatttaa cccaaacacg
    11041 ttcaccagaa tcattataca tagagtatgt gtgagaaccg aacccatgca tatgacgtaa
    11101 atctttagga atccctctat ctgacattaa gatcgttact tggtgcaatg cttctggaag
    11161 acccgtccag aaatcccagt tattttgtgc atctctcata tttgttctag gatctcgttt
    11221 caccgcacga tttaaactaa caaataactt tggatctcta aagaagaata ctggtgtgtt
    11281 attccctact aaatcccagt tcccttcttc agtgtagaac tttaacgcaa atcctcgaat
    11341 gtcacgctcc gcatcagcag caccacgttc tcctgctaca gtagagaaac gggcaaacat
    11401 ttcggtttgc ttacctattt cagagaatat tttagcattc gtatattttg ttatatcttt
    11461 agttacagta aatgtcccaa atgcaccaga acctttggca tgcattcgac gttctggtat
    11521 tacttctcta tcaaattgag acatttgctc taaaaagtaa atatcttgca ttaaaagagg
    11581 tcccctaggc cctgctgtca tacta
//
LOCUS       DAOBBH010000073        11538 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.073, whole genome shotgun sequence.
ACCESSION   DAOBBH010000073 DAOBBH010000000
VERSION     DAOBBH010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11538
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.073"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(224..319)
                     /locus_tag="P6L01_001407"
     CDS             complement(224..319)
                     /locus_tag="P6L01_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030481.1"
                     /translation="MGGFVNSLIMKDLSDNDMRFKYEYYNREKDT"
     gene            complement(395..1000)
                     /locus_tag="P6L01_001408"
     CDS             complement(395..1000)
                     /locus_tag="P6L01_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HDP3030482.1"
                     /translation="MNMPLMIDLTNKNVVIVGGGVVASRRAQTLNQYVEHMTVISPTI
                     TEKLQNMVDNGVVIWKEKEFEPSDIVDAYLVIAATNEPRVNEAVKQALPEHALFNNVG
                     DASNGNVVFPSALHRDKLTISVSTDGASPKLTKSIMAELEALYPPSYSSYIDFLYTCR
                     QKIKVLDITYNEKQQLLSQIVSQEYLNHDKQAQFLAWLDVR"
     gene            complement(1063..2943)
                     /locus_tag="P6L01_001409"
     CDS             complement(1063..2943)
                     /locus_tag="P6L01_001409"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HDP3030483.1"
                     /translation="MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDT
                     TSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMR
                     LAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
                     EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDALLENLGAERICKRVDCDIDYEED
                     AEKWMADIINMIDTTSEGIQSESVISESIKSAKEKKYSKLNPYQAEVLANINLNGTDS
                     NKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLISMLGWDPQSPVPINDRGDT
                     VPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITD
                     FPTIELQPENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSV
                     HFAERIKPGDIVPIYLKKNPNFKFPMKQDIPVIMIGPGTGIAPFRAYLQEREELGMTG
                     KTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIMEESERF
                     NEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRD
                     VY"
     gene            3137..3229
                     /locus_tag="P6L01_001410"
     CDS             3137..3229
                     /locus_tag="P6L01_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030484.1"
                     /translation="MSVINAYSNASLLFFIYLLFIMYQISLSVK"
     gene            complement(3337..3834)
                     /locus_tag="P6L01_001411"
     CDS             complement(3337..3834)
                     /locus_tag="P6L01_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDP3030485.1"
                     /translation="MNNRNVYDIEVSDYKGLTYKLEAFRGKVILVVNTATECIYSEQL
                     KKLETLFQKYKDRGFVVLSFPNNNFDNRQPGSNEEILKISREKFGVTFPVLAKISVNG
                     NDEHPLFTHLKNEQPGIFGSPIKWNFTKFIIDRQGNVVNRFLPMEDPLDISTNIEILL
                     QESSS"
     gene            complement(3844..4104)
                     /locus_tag="P6L01_001412"
     CDS             complement(3844..4104)
                     /locus_tag="P6L01_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030486.1"
                     /translation="MIIMRGYNERYCSWTNTAIQCDYFNKVRQVKKDAKSDRLSNNIN
                     SNIIYRVLEKCITLYNVCSRCKRALILSEHSKKNIFNIKKAV"
     gene            complement(4486..4599)
                     /locus_tag="P6L01_001413"
                     /pseudo
     CDS             complement(4486..4599)
                     /locus_tag="P6L01_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501402.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5258..7257)
                     /locus_tag="P6L01_001414"
                     /pseudo
     CDS             complement(5258..7257)
                     /locus_tag="P6L01_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501404.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(7254..8009)
                     /locus_tag="P6L01_001415"
     CDS             complement(7254..8009)
                     /locus_tag="P6L01_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3030487.1"
                     /translation="MLLEVKHVKKVYGKGLNATTALNQMNLSVGAGEFVAIMGESGSG
                     KSTLLNLIASFDGLTEGDIIVDGAHLNNMKNKSKALYRQQMVGFVFQDFNLLPTMTNK
                     ENIMMPLILAGAKRKDIEQRVHQLAVQLHLEGFLNKYPSEISGGQKQRIAIARALVTK
                     PTILLADEPTGALDSKTSKALMMLFQEIHQLEQTILMVTHSNIDASYAERVIFIKDGR
                     LYHEIYRGEESQLAFQQRITDSLALVNGGSVNI"
     gene            complement(8117..9004)
                     /gene="nsaS"
                     /locus_tag="P6L01_001416"
     CDS             complement(8117..9004)
                     /gene="nsaS"
                     /locus_tag="P6L01_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system sensor histidine kinase NsaS"
                     /protein_id="HDP3030488.1"
                     /translation="MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILL
                     LLLIFIGIKYLSFVKTISQQQQIENLETALYQLKNEQIEYKNDVESYFLTWVHQMKTP
                     ITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIR
                     PIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLMIEQLINNALKYARGKDIWIEFD
                     EQSNQLHVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPV
                     SVVSKQNEGTTFTIQFPDE"
     gene            complement(9015..9680)
                     /gene="nsaR"
                     /locus_tag="P6L01_001417"
     CDS             complement(9015..9680)
                     /gene="nsaR"
                     /locus_tag="P6L01_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system response regulator NsaR"
                     /protein_id="HDP3030489.1"
                     /translation="MKILIVEDDFVIAESLASELKKWNYGVIVVEQFDDILSIFNQHQ
                     PQLVLLDINLPTLNGFHWCQEIRKTSNVPIIFISSRIDNMDQIMAIQMGGDDFIEKPF
                     NLSLTIAKIQALLRRTYDLSVANDSLTVKGCTLILDEAKVVYQEQNIQLSLTELQILK
                     LLFQNEDKYVSRTALIEKCWESENFIDDNTLAVNMTRLRKKLNTIGVNDFIITKKNVG
                     YKV"
     gene            complement(9706..9906)
                     /locus_tag="P6L01_001418"
     CDS             complement(9706..9906)
                     /locus_tag="P6L01_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030490.1"
                     /translation="MILVMLSPLLIIFFIVLSILEERKRTKKKQLEKEKANTLNQNTN
                     DTESSNQEPSLQQTKEQKDNKG"
     gene            10104..11528
                     /locus_tag="P6L01_001419"
     CDS             10104..11528
                     /locus_tag="P6L01_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDP3030491.1"
                     /translation="MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVG
                     NTKNPKNVIFLVGDGMGPSFNTAYRYYKNDSHAKEMTPTAFDSYLKGTNRTYSNDPKQ
                     NITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
                     PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDG
                     YDLATNSKDLSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGF
                     FLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGL
                     TIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKE
                     NKKLQKLLKKEKDEKSADVEKQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYG
                     PRSESFSGNIENTESAKIIFDIFK"
BASE COUNT         3741 a         2015 c         1652 g         4130 t
ORIGIN      
        1 tttatcaaaa aaatcttttt tgcgtaacta tcaatagtga agtttgattt tcctacttga
       61 taacatggca tgactaatcc ttgtatatcc tgtcctgtat ttcattttga tctatcattt
      121 ttattattgc tttctattgc acaaaaaatc tggctaaagg tcgctatgat agtaacgact
      181 tcaaccagat ttcaatttat ccatctattt tattaagttt gttctacgta tctttttctc
      241 tattgtaata ttcatattta aaacgcatat cattatcgct taaatccttc attattagac
      301 tattcacaaa gccacccatg tcttaatttc aatgattaag cgttaaagtt aaaataagaa
      361 gacttacctt agacggttag acggtccgct attattatct tacatccaac cacgctaaaa
      421 attgagcttg tttgtcatga tttaaatatt cttgtgacac aatttgtgac agtaactgtt
      481 gcttttcgtt atatgttata tcaagtactt ttattttctg tcggcaagta tataaaaagt
      541 cgatatacga actgtatgat ggtggatata acgcctcaag ctctgccata attgattttg
      601 tcaacttagg actcgcacca tcagttgata cactgatagt tagcttgtcg cggtgtagtg
      661 cacttggaaa tacaacattg ccatttgatg catctccaac attattaaaa agggcatgct
      721 caggtaaggc ttgttttacc gcttcattga cacgtggctc attggttgct gcaataacta
      781 gatacgcgtc tacaatatcg cttggttcaa attctttttc tttccatatg acgacaccgt
      841 tatctaccat attttgaagt ttttcagtga ttgtcggact gatgaccgtc atatgttcaa
      901 cgtattgatt taatgtttgt gcccgacgac ttgcaacgac gcctccacca actatgacga
      961 catttttatt tgttaaatct atcattaatg gcatattcat gttgagcaac atcctttata
     1021 tctttttaca tcattacacc ttaaaatatt taacaccaat cgctaataaa catcacgttg
     1081 atagcgttgt tgttgtttca tttgtcgcaa taataactct gcttcttctt gagaaatatg
     1141 acgttctttt accaatacat ctttaatggc ttgatggaca tctttcgcca tacatttttc
     1201 atcgccacaa atatagattg ctgcgccttg ctcaatccat tcattgaaac gttcactttc
     1261 ttccattata cgatgctgta catattcttt gtgttcttgg tctcttgaaa atgctaaatc
     1321 tacgcgtgtt aagtttccat tttcaagcca ttcttctatt tcttcttcat ataaaaagtc
     1381 agaactacgg tgttgatcac caaagaacaa ccatgttttt ccagtcatac caagttcttc
     1441 acgttcttgt aaatatgctc taaaaggagc aattccagta cctggtccaa tcataataac
     1501 cggtatatct tgcttcatcg gaaatttgaa gttcggattt ttcttcaaat aaattggtac
     1561 tatatcgcct ggtttaattc gctcagcaaa atgaaccgag catacacctt ttctctcacg
     1621 tccatgtgct tgataacgaa ccgtaccaac ggtaatatgc acttcatctg gcgttgccat
     1681 aaaactacta gaaatcgaat actctcttgg tggtaatttt ctaaggattt gatacatatt
     1741 ctcaggttgt aattctatag ttggaaaatc tgttattaaa tctataaagt cccgatttat
     1801 aacatattca cgcgcccatg actcatcttg aatacgttca gataattctt cattgtcaaa
     1861 atagatatct gcatttttca ataatggcaa tgttaattta gtaaattcaa aatgtgatgt
     1921 tagtgcttca acaataggaa ctgtatcacc acgatcatta attggcaccg gagattgcgg
     1981 atcccaacct aacatggata ttagtttttc aaccaattca gggtcgtttt gcggtaatgc
     2041 tactatacaa tctcctggtt catatgattc actaaaatca tcaagtaaaa attctatatg
     2101 tcgtgtttct ttatttgaat cggtaccatt taaattgata ttcgctaaaa cttctgcttg
     2161 gtatggattt aatttagaat atttcttttc tttggcagac ttaattgatt cacttatcac
     2221 cgattcactt tgaatacctt ctgatgtagt atcaatcata ttaatgatgt ctgccatcca
     2281 cttttctgcg tcttcttcat aatcaatatc acaatctaca cgcttacata tacgctcagc
     2341 gcctagattt tctagtaaag catctacatc tttaccagct tgacagaaaa attcatatgt
     2401 ttgatcacct aaagctagta ctgaatatct cacatgattt aaattaggtg cgttatcgtc
     2461 ttctaaaaat tcaaagaaat cccatgcatt atcaggcggt tctccttcac catgagtaga
     2521 cgtaataata aataaatctt ctaactgtgc gatgtttgtc gtatcatatt catccattga
     2581 catcaaaaca acttgatgtc cgatatcact taaacgttct gaaaaaattt cagctaaacg
     2641 catggcatta cctgattcag aaccatatag cactgtaaca tgccgctgat tagcttcgat
     2701 atgtggctct ttattttgta acatatacgt tgttgaagtt gcttgttctt ctgatatttg
     2761 tgattgttgc cacacttctg tctctaattg ttgttgatta ctatcacttg tcgtatcgtt
     2821 actattagct agtaaaaaac cactaagcca ctgttgttgg ctctctgtta atgtttgcag
     2881 cagattgtta atctctgtga cttgtttttc tgtaaatgga ctattagatg tatttaattt
     2941 caaatgtttc acctcaataa acttgaaata gatgattgac tcttttttta tcgatagttt
     3001 agtcaatttt ttaatatgta tggtataaat taaaacaaac ttgagttgtg tctgatagtt
     3061 atacttgcaa atatttacaa ctttcatgtt gcttgaatga cattatcagt aattcagaca
     3121 aaactttgat tatgtcgtgt cagttattaa tgcatacagt aatgcatcgt tgttattttt
     3181 tatttattta ctatttatta tgtaccaaat cagcctttca gtaaaatagg tcaaattact
     3241 gattttctaa atataaaatg cctcctaatg acatactatt agtacatcat taagaggctc
     3301 ttgtgttatt tgcatactaa gcgctcaatt ttaaatttaa gatgaagatt cttgcaataa
     3361 tatttctata tttgtcgata tatccaatgg atcttccatt ggcaagaatc gatttacaac
     3421 attgccttgt cgatcgatta taaattttgt gaaattccat tttattgggg acccaaagat
     3481 tcctggttgt tcattcttta aatgcgtaaa taacggatgt tcatcgttcc cgttcacaga
     3541 tattttagct agcactggaa atgtaacacc aaatttctca cgactaattt tcaagatttc
     3601 ttcattagaa cctggttgtc gattgtcaaa attattattc ggaaaactca acactacaaa
     3661 cccacgatcc ttatattttt gaaacaaagt ctctagtttt ttcaattgtt cgctatatat
     3721 acattctgtt gcagtattaa caactaaaat cactttacct ctaaatgctt ctaatttata
     3781 agttaagcct ttataatcac ttacttcgat atcatacaca tttctattat tcataagaca
     3841 cccctacaca gcctttttta tattgaatat attcttttta gaatgttctg ataaaataag
     3901 tgcgcgttta caccgcgaac atacattata tagcgtgata catttttcga gcacacgata
     3961 aataatgttc gagtttatgt tgttgctcaa cctatccgat ttagcgtctt ttttcacctg
     4021 tcttacttta ttaaaataat cacattgtat agctgtatta gtccagctac aatatctttc
     4081 gttataaccc ctcattataa tcatccttat tttctatttt taaaaagaca attagaccgc
     4141 tctttaaact atagattaat acttaagtta aactcataca tactgatacc atacgttaga
     4201 tctaacaatt taaaattcgt tataactatg gattaaagag ctgcccaact catataatcc
     4261 ttaaaaactt cacatgtgat tgtttattaa gccctccttt atcttattaa atatccttat
     4321 aaccctttta aaattaaact gacacactca tacattgtta cacgcgacga taaacgtcat
     4381 attattgcat atcattaaaa caagtcactt tgtatcgtgt gcgaccgaat acatattaaa
     4441 tcatcaataa tgattattca attattaaaa ataaaaacga acattctata tagagtttct
     4501 acttattgtt ttttcagcat tgtctacatt acttaacatt cgtgtttata agtaatattg
     4561 accgccaata tttagacact ttataagtat gccattcatc atttttaatc atattatttt
     4621 tcttattgat atttctattc atatatttct gattgtatat ttagatataa taatttgtta
     4681 ttggaaaata tatgaaacaa atttgactca tctttttcaa tatctaaatg acacgatgca
     4741 tgatttttga attttgtaat taacttcctt ttaaattaga ctgaattgtg acaattttca
     4801 aacattttaa tgtgtaaatc attggcaaaa tatacgaatt gcatttaaat cgtcaattta
     4861 catatatttt tgcgtagtaa tcaatcgtta tcattgttat ttatcgttac attatttcga
     4921 gtatcagtat gtatttcggg cttcgtttga taacgacatt tctttgtgac atcgcttcat
     4981 cagtgtaaca acaaatacaa tgatttcgtg atgttagtta cccattttat gtgttgcata
     5041 aaatatgttg ttataaaaca ttttaaatca ttttatataa acaatctata tatttttggt
     5101 atttccaaaa tatcacttgt tatattaaaa accgacaaga cattttatct tatcggttca
     5161 aatttggtat tgttatttgt aatgttttta ggtttctttt taatataata tatttcagtg
     5221 aaaatacatg attgattgtg atttcactga aacatggtta attacgttgt tgatgaataa
     5281 ctttagcata aatataggaa gttattttgt acatcgccat atatagaaac gaaattataa
     5341 tgacagctag tacgtaactt gttaaaaata tatgatggtt attaatacct atcatattta
     5401 gtaacgtata tacaatgtta ctagaaatta atgtgtgaat cagtgctact gttattggta
     5461 ttgcgaacaa gaaagtcatt tgatttcgtg ttatctttgc tattcttcca ttatctaaac
     5521 caagtttttt cataatgtca taattgtgct gatttgcata ggcttctgag acttgtttat
     5581 agtacatcat tagaaatatt ccaatgacaa gtacaattga tactacgcca cctacaaata
     5641 ttagtccacc tgtcaaacga tcccaaacca ttagcatttc tgatttagaa tttattgacg
     5701 catcgtattt tttcgatagc ttttgtattt gtgatgttgt taatttcgtc tttccaaata
     5761 cattaaagtt tatcgatatt gtcgaattct tttgttcatg tgcataaaat ccttttactt
     5821 tttgtaattg attgttatct ttaacaatta aaaccatact atcttgaatc atcatcaaat
     5881 ttaacgcatg tgcgtcaagt tgtttcactt taaagacttc attattaaga ccgaccttag
     5941 actgcttttt aaaaataggt acattcgtaa tcatacctat actttgattg tctaagtgta
     6001 atttgttttg gtgatatcga ttataatctt gtgcagtaaa agctgtaatc attactgatt
     6061 gtcttgaaaa tatcgcgcct tcattaccaa tgaagtctat tggaatatca tcactagcct
     6121 gttttaactt gtaataagca cgattctttt ctaaagaagc tctaaataat gtcgtttcat
     6181 atactttgaa gtcttttgca ttaaccgaac cctgtatatc acgtttaata ttctcaattg
     6241 tttgttgacg ttctatttgt gacttagaat tgtcgctaaa tgacaaatca taatcattcg
     6301 taatcaattt cgtaatggta tgattcatat cacgataggt tgttaatgtc attgtgagcg
     6361 ttacaatcaa aatgtacaca gcaaagtgat tgttgcaaga ctgactgcat tcattttaag
     6421 acgtacacgc atcccaacta ccacaaaaaa ataccgtgga tgatagtaaa cttttgatac
     6481 ctgctgcaat atactaataa ttatttcact tatacctaca aaaaatgcat aagtaccgat
     6541 aataactaat cctatgacaa tccatatttt aaaaaaggca ccaaacgtcg tatcttgttg
     6601 taatgcaata aagtaaccta aatatagtgc tgcgcttcct ataacaatta aaacgtaacg
     6661 caaccatcgt gatatgacac tagaatcgga acgatgttga taagttatcg gccgttgaaa
     6721 attaatactg aacaagttga atataagtag catgcccatt aatacagcaa tgataatcaa
     6781 agtaataaac atcgctacag agtcaaatgg ataatacctt aacgtcgcaa cttcactgcc
     6841 cattattttc tgtataaaca agaaaaatat cgcaccaagt aagtatccgc cggcaatact
     6901 aacgactgaa attatcacaa attgtaagat agtttccatc actacaatta aacgcatact
     6961 tttcttggtc atgcccaatg tcataaaaat gctaaactct tttcgtcttt gtgacatcat
     7021 aaagtgattt gcatagaaaa taaaaataaa agttaaaagc gccataaaaa aattagctat
     7081 gataataaat ggtactagga agtcattctt ctgttttatg tagctattta acccaattga
     7141 cacaagcgta tattcaatca tgaatagtac acttaccgct aaaatgaatg gaatgattac
     7201 aagtcgctgc gtaataaatt gacgtcgcac tatgtgccat aacaatctta acttcatata
     7261 ttgacacttc ctccattcac aagtgctaag ctatctgtta ttcgttgttg aaaagctaat
     7321 tgactttctt caccacgata tatttcatga tatagacgcc catctttaat aaaaatgact
     7381 cgctctgcat aagacgcatc gatatttgaa tgagttacca ttaaaattgt ctgttccaat
     7441 tgatgaatct cttgaaataa catcatcaat gcctttgacg tttttgaatc aagtgcacct
     7501 gtaggttcat cggctagtaa aatcgtcggc ttagtaacta atgcacgtgc aatggcaatg
     7561 cgttgcttct gacccccaga gatttcagaa ggatacttgt ttaagaatcc ctctaaatgt
     7621 aattgtactg ccaactgatg taccctttgt tctatatctt ttcgtttagc accagctaaa
     7681 attaatggca tcattatatt ttctttattc gtcattgttg gtaaaagatt aaaatcttga
     7741 aaaacaaaac ctaccatttg ttgacgatac aatgctttac ttttattttt catattattt
     7801 aaatgtgcgc catccacaat aatgtcacct tcagttagtc catcaaaaga agcaattaaa
     7861 tttagtagtg tagacttccc tgacccagac tcacccataa ttgcaacaaa ttcaccagct
     7921 ccaactgata aattcatttg attaagtgct gtcgtagcat tcaaaccttt accataaacc
     7981 ttttttacat gtttcacttc tagcaacatg gcatgcacct catttctttg ttagttctat
     8041 attaaaacaa aaaaatacta ataacattta aattagtaaa aaaatgttac tagtatactt
     8101 acaatattga aagtttttat tcatctggaa attgaatcgt aaatgttgta ccctcatttt
     8161 gtttagatac gactgaaaca ggatggtttg tgtgtgttga aatttgtttt acgataaata
     8221 aaccaatccc acttgagtta ctttggcgct ggccattata acctgaatag cccttatcaa
     8281 atattttagg caagtccgct tcactaatac cgataccatt atcttttacg tgtaattgat
     8341 tggattgctc atcaaattca atccatatat ctttacctct cgcatactta agtgcattat
     8401 ttattaattg ttctatcatt aaagaggtcc atctaacgtc agttaatact tcgtgatgac
     8461 aaggttcata atggattttt gttttttgat caataaactg tattgaatat ttcataataa
     8521 ttgggcgaat gatattatta atcgaaattt tagtgacaga aatatcagaa gtttcattta
     8581 ataactttaa ataactaagt gctaaacttg tatagttatc aatttgaata acctcttgac
     8641 gaacacgatt aacaacatta ggctcatctc tttcaagtaa cagttgtgct gcagtaatgg
     8701 gtgttttcat ttgatgtacc catgttaaaa agtagctctc tacatcattt ttatattcaa
     8761 tttgttcatt tttaagctga tacaacgcag tttctaagtt ttcaatttgt tgttgttggc
     8821 ttatagtttt tacaaaactt aaatatttaa tacctatgaa tatgagtaat aataataaaa
     8881 taacgccaag tgctagtaaa tatgcttcta atggcaaatg atacaggtaa aacattaagc
     8941 caaataaagc aaaaataact atgactattg ctatttcctg agtaatactt ttaagaaagg
     9001 tcattgcatt caccctatac tttatatccg acatttttct ttgtaatgat aaaatcatta
     9061 acgccaatag tatttaattt tttacgcagg cgcgtcatgt taacagctaa tgtgttatca
     9121 tctatgaagt tttctgattc ccaacatttt tcaattaaag cagttctact tacatattta
     9181 tcttcatttt gaaataataa ctttaatatt tgtaattcag tcaaagatag ctgtatgttt
     9241 tgttcttgat acacgacttt tgcttcatct aatattaatg tacaaccttt aaccgtcaat
     9301 gaatcattag ctactgacaa gtcataagtt cgtctcaata atgcttgaat tttggcaatc
     9361 gttaatgaca agttaaatgg cttttcgata aaatcatctc cccccatttg tattgccata
     9421 atttggtcca tattatcaat acgggaacta ataaatataa ttggcacatt agatgttttt
     9481 cggatttctt gacaccaatg aaaaccattt aacgttggca aattaatatc tagcaataca
     9541 agctgaggtt gatgttggtt aaagatagac agtatatcat caaattgttc aacgacaata
     9601 acaccgtaat tccatttttt aagttcagat gctaaacttt ctgctataac aaaatcatct
     9661 tcaacaatta atattttcat ctataatctt cttccttcaa ttgaattatc ctttgttatc
     9721 tttttgttct ttagtctgct gcaatgacgg ctcttgattt gaactttccg tgtcatttgt
     9781 attttgattt agtgtatttg ctttttcttt ctcgagttgc tttttcttcg tacgtttacg
     9841 ctcctctaaa atagacaaca ctataaagaa tatgattaat aatggagata acattactag
     9901 tatcattttt ccacctcaaa ttatatttaa ttctatattt tcacacgctt ctattctaac
     9961 gctttctata acaaattgtc atatatattc attttttgtt attctaaatc actctgttta
    10021 catattttta actaaaacta cataattgct caaaattcct cttttacact gaagacatag
    10081 tcatatactc aggaggatgt attatgttat cagttaaaaa agccacaaaa tttgcagttg
    10141 ctaaccttat tatcgtgtca acagttttca gttctgtcac acctgcaatt gcgcaatccg
    10201 ataaaagttc taaagatgaa agtttcgcgg ttggtaatac taaaaatcca aaaaatgtta
    10261 tctttttagt tggcgatggc atgggtccat catttaacac agcttatcgt tattataaaa
    10321 atgattcgca tgccaaagaa atgaccccta ctgcttttga ttcatattta aaaggcacaa
    10381 atcgtacata ttcaaatgac ccaaaacaga atattactga ttctgcagct ggcggaacag
    10441 cctttagttc tggccataag acatataatg gtgccatcgg tgttgacagt aacaagcaga
    10501 aagtaaagac agtgcttgaa cgtgcaaaag aaaaaggtaa gtcgactggt cttgtttcaa
    10561 cagctgaatt aacagatgca acaccagcag catatgctgc acacgtgact tctagagatg
    10621 ataaaaacga aatcgctaaa caattttata aagataaaat caacggaaag cataaagttg
    10681 atgttttatt aggcggcggc gctaaatatt tcggtaaaag taatggcaat ttagataaaa
    10741 aattcaaaaa agatggttac gaccttgcca cgaacagtaa ggatttatca aaatctgata
    10801 aagataaagt tcttggtttg tttgcagata aaaatatgcc acttgcaata gatgcttcta
    10861 aagacgaacc atcattagct gatatgcaac aaagtgcact tagtaagctt gagcgtaata
    10921 aaaaaggttt ctttttaatg gtcgaaggtg cttctataga taaatcagcg cattccaacg
    10981 atattacagg cgttatgtct gaaatggaag gttttgaaaa agcattcgat gacgccattc
    11041 aatatgctaa gaaacataaa gatacacttg tcgttgcaac agcagatcac tctactggtg
    11101 gtctaacaat tggtaaagat aaaggatacg aatggaatcc tcaaccgatt aaatcgatga
    11161 aacactctgg atcatatatg acagaaaaaa tggctaaagg tgaagaccct gaaaaagtta
    11221 ttaatgaagg ttatggcttt gaattttcac aagatgatat gaaaaaggtt aaaaaagaga
    11281 ataaaaagtt gcaaaaatta cttaaaaaag aaaaagacga aaaatctgca gatgtagaaa
    11341 aacaaacaaa agccttacaa cacgcaattc agaagccagt aaatgataaa tcttatacag
    11401 ggtggacttc cgatggacat acaggcgagg atgttaatac ctacgcttac ggacctagat
    11461 cagaatcatt tagtggtaat atcgaaaata cagaaagcgc aaaaataata tttgatatat
    11521 tcaagtaaag cataaaaa
//
LOCUS       DAOBBH010000074        11367 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.074, whole genome shotgun sequence.
ACCESSION   DAOBBH010000074 DAOBBH010000000
VERSION     DAOBBH010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11367
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.074"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            177..1052
                     /gene="rsgA"
                     /locus_tag="P6L01_001420"
     CDS             177..1052
                     /gene="rsgA"
                     /locus_tag="P6L01_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDP3030492.1"
                     /translation="MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIV
                     EFEVQNINEGYIHQVFERKNELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHS
                     YQLNARVLVTKKDKTPIEKQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLI
                     VLSGQSGVGKSTFLNHYRPELNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGF
                     SALDFDHIDKDEIKDYFLELNRYGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDH
                     YLQLFNEISNRKVRY"
     gene            1053..1697
                     /locus_tag="P6L01_001421"
     CDS             1053..1697
                     /locus_tag="P6L01_001421"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDP3030493.1"
                     /translation="MTKLYPSLLSVDFLDLQHELKRLEEAGVDGVHFDVMDGQFVPNI
                     SIGLPILDAVRKGTTLPIDVHLMIENPEKYIASFAEHGADMISIHVESTPHIHRAIQM
                     IKHLDKKAGVVINPGTPISQIEPILDIVDYVLVMTVNPGFGGQSFIDQCVEKIAGLNA
                     IKMERQLNFDIEVDGGVNTDTAKVCVENGATMLVTGSFFFKQEDYKKVTQALKG"
     gene            1704..2345
                     /locus_tag="P6L01_001422"
     CDS             1704..2345
                     /locus_tag="P6L01_001422"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDP3030494.1"
                     /translation="MHINLLCSNRHLPQDIWAKSNEGKWGGVDRGALILLKHQIIPFF
                     SVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGR
                     LDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYPYISFIPMTD
                     DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSTDLN"
     gene            complement(2726..2914)
                     /locus_tag="P6L01_001423"
     CDS             complement(2726..2914)
                     /locus_tag="P6L01_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDP3030495.1"
                     /translation="MGKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKP
                     KKVWVSARALKSGKVTRV"
     gene            3357..3731
                     /locus_tag="P6L01_001424"
     CDS             3357..3731
                     /locus_tag="P6L01_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDP3030496.1"
                     /translation="MTLEISNDYGKIDISNEVIASVVGGKAVECYGIVGMASRQQVRD
                     GIAEILGHENYAKGIKVTENNGVVDIDMYIIVSYGVKISEVANNVQSTVKYTLEKSLN
                     VSVNSINIYVQGVRVNNTGKKA"
     gene            3746..5392
                     /gene="fakA"
                     /locus_tag="P6L01_001425"
     CDS             3746..5392
                     /gene="fakA"
                     /locus_tag="P6L01_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase catalytic subunit FakA"
                     /protein_id="HDP3030497.1"
                     /translation="MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTN
                     MNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESES
                     EINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAIEKANNTEDCIELMEYI
                     IVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKIDKDE
                     FVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDE
                     EIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETA
                     IITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNIL
                     MASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKVQMADSVNNVKSGSLT
                     YAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLAEDSEILTVIIGQ
                     DAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE"
     gene            5582..7642
                     /gene="recG"
                     /locus_tag="P6L01_001426"
     CDS             5582..7642
                     /gene="recG"
                     /locus_tag="P6L01_001426"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDP3030498.1"
                     /translation="MAKVNLIESPYSLLQLKGIGPKKIEVLQQLNIHTVEDLVLYLPT
                     RYEDNTVIDLNQAEDQSNVTIEGQVYTAPVVAFFGRNKSKLTVHLMVNNIAVKCIFFN
                     QPYLKKKIELNQTITVKGKWNRVKQEITGNRVFFNSQGTQTQENADVQLEPVYRIKEG
                     IKQKQIRDQIRQALNDVTIHEWLTDELREKYKLETLDFTLNTLHHPKSKEDLLRARRT
                     YAFTELFLFELRMQWLNRLEKSSDEAIEIDYDLDQVKSFIDRLPFELTEAQKSSVNEI
                     FRDLKAPIRMHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLM
                     ALFGDSMNVALLTGSVKGKKRKILLEQLENGTIDCLIGTHALIQDDVIFHNVGLVITD
                     EQHRFGVNQRQLLREKGAMTNVLFMTATPIPRTLAISVFGEMDVSSIKQLPKGRKPII
                     TTWAKHEQYDKVLMQMTSELKKGRQAYVICPLIESSEHLEDVQNVVALYESLQQYYGV
                     SRVGLLHGKLSADEKDEVMQKFSNHEIDVLVSTTVVEVGVNVPNATFMMIYDADRFGL
                     STLHQLRGRVGRSDQQSYCVLIASPKTETGIERMTIMTQTTDGFELSERDLEMRGPGD
                     FFGVKQSGLPDFLVANLVEDYRMLEVARDEAAELIQSGVFFENTYQHLRHFVEENLLH
                     RSFD"
     gene            7845..8417
                     /gene="fapR"
                     /locus_tag="P6L01_001427"
     CDS             7845..8417
                     /gene="fapR"
                     /locus_tag="P6L01_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HDP3030499.1"
                     /translation="MRGETLKLKKDKRREAIRQQIDSNPFITDHELSDLFQVSIQTIR
                     LDRTYLNIPELRKRIKLVAEKNYDQISSIEEQEFIGDLIQVNPNVKAQSILDITSDSV
                     FHKTGIARGHVLFAQANSLCVALIKQPTVLTHESSIQFIEKVKLNDTVRAEARVVNQT
                     AKHYYVEVKSYVKHTLVFKGNFKMFYDKRG"
     gene            8422..9408
                     /gene="plsX"
                     /locus_tag="P6L01_001428"
     CDS             8422..9408
                     /gene="plsX"
                     /locus_tag="P6L01_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDP3030500.1"
                     /translation="MVKLAIDMMGGDNAPDIVLEAVQKAVEDFKNLEIMLFGDEKKYN
                     LNHERIEFRHCSEKIEMEDEPVRAIKRKKDSSMVKMAEAVKSGEADGCVSAGNTGALM
                     SVGLFIVGRIKGVARPALVVTLPTIDGKGFVFLDVGANADAKPEHLLQYAQLGDIYAQ
                     KIRGIDNPKISLLNIGTEPAKGNSLTKKSFELLNQDHSLNFVGNIEAKTLMDGDTDVV
                     VTDGYTGNMVLKNLEGTAKSIGKMLKDTIMSSTKNKLAGAILKKDLAEFAKKMDYSEY
                     GGSVLLGLEGTVVKAHGSSNAKAFYSAIRQAKIAGEQNIVQTMKETVGESNE"
     gene            9401..10327
                     /gene="fabD"
                     /locus_tag="P6L01_001429"
     CDS             9401..10327
                     /gene="fabD"
                     /locus_tag="P6L01_001429"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDP3030501.1"
                     /translation="MSKTAIIFPGQGAQKVGMAQDLFNNNDQATEILTSAAKTLDFDI
                     LETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVL
                     SFEDAVKIVRKRGQLMAQAFPTGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANIN
                     CPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFE
                     WRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPG
                     KVLSGLIKKINRDVKLTSIQTLEDVKGWNEND"
     gene            10320..11054
                     /gene="fabG"
                     /locus_tag="P6L01_001430"
     CDS             10320..11054
                     /gene="fabG"
                     /locus_tag="P6L01_001430"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDP3030502.1"
                     /translation="MTKSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVV
                     EEIKAKGVDSFAIQANVADADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQ
                     EWDDVIDTNLKGVFNCIQKATPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVI
                     GLTKSAARELASRGITVNAVAPGFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIAN
                     TVAFLASDKAKYITGQTIHVNGGMYM"
BASE COUNT         4128 a         1496 c         2249 g         3494 t
ORIGIN      
        1 ttatgttgag attgcggtag ttttaaaatg cttaatgtca tcatttacag ttgagcattg
       61 tctatcaatt ctgtatcact ctcacaatat ttatatcatt tgaaatgatc atttatatat
      121 taattttcca gtctttgata taataaatgc agtaaaatta atcgagaggt gccattttga
      181 agacaggtcg aatagtgaaa tcaattagtg gggtatatca agtagacgtt aatggcgaac
      241 gtttcaatac aaaaccacga ggattattta gaaagaaaaa attttcaccg gtagttggtg
      301 atatagtgga atttgaagta caaaacatta acgaaggcta tattcatcaa gtgtttgagc
      361 ggaaaaatga gttgaaaaga ccacctgtaa gtaatataga tacactagta attgtaatga
      421 gtgctgtcga gccaaatttt tcaacgcaat tattagatcg atttttagtt attgcacatt
      481 cgtatcagtt aaatgcgaga gttttggtga ctaaaaaaga taaaacacca attgaaaagc
      541 agttcgaaat taatgagttg ttgaaaatat atgaaaatat tggctatgag actgaattta
      601 ttggaaatga tgatgatcga aaaaaaattg tagaagcttg gccagctgga cttatagtac
      661 ttagtggtca atcaggtgtc ggtaagtcca ctttcttaaa tcattatcgt ccagaactta
      721 atcttgagac aaatgatata tcaaaatcat taaatcgagg aaagcatact acaagacatg
      781 tcgaactatt cgaacgtcaa aacggttata ttgcagacac acctggattc agtgctttag
      841 attttgatca tatagataaa gatgaaataa aagattattt tcttgaatta aatcgatatg
      901 gtgaaacatg taagtttagg aattgtaatc atatcaaaga acctaattgt aatgttaagc
      961 atcaattaga gatagggaat attgcgcaat ttagatacga ccattattta caactattta
     1021 atgaaatttc aaatagaaag gttagatatt aaatgacaaa actatatcca tcattattat
     1081 ctgttgattt tttggattta caacatgaat taaaacgact tgaagaagca ggtgtcgacg
     1141 gagttcattt tgatgttatg gatggtcaat ttgtgcctaa tatatctatt ggtttaccaa
     1201 tattagatgc agtaagaaaa ggcacaacat tacctataga cgtacatttg atgattgaaa
     1261 atccagaaaa gtatattgca tcatttgcag aacatggtgc cgatatgatt tcaattcatg
     1321 tcgaatcaac gcctcatatt catcgtgcta ttcaaatgat taaacattta gataaaaaag
     1381 ctggtgtagt aattaatcct ggtacaccaa tatcacaaat tgaacctatt ttagacattg
     1441 ttgattatgt attagtgatg acagttaacc cagggtttgg tggtcaatca tttattgatc
     1501 aatgcgtaga aaaaatagcg ggtcttaatg ctattaaaat ggaacgtcaa ttaaactttg
     1561 atattgaagt tgatggaggc gtaaataccg atacagcgaa agtttgtgtt gaaaatggtg
     1621 ctacaatgct agtaacaggt tcatttttct ttaaacaaga agattataaa aaagtcacac
     1681 aagcattgaa aggttgagtg tatatgcata taaatttatt atgttctaat cgacacttgc
     1741 cgcaagatat ttgggccaaa agtaatgaag gtaaatgggg cggcgttgat agaggtgctt
     1801 tgattttatt gaagcatcaa attatccctt ttttctcagt gggagacttt gattcagtca
     1861 gtaaagaaga acgccaactt ctaacagaac agttacaaat caaaccagtt caagctgaaa
     1921 aagctgatac ggatttagct ttagcggttg ataaagctgt tgcacttgga tttgatagta
     1981 ttacaattta tggtgcaaca ggcggacgat tagatcactt ttttggggca attcagttat
     2041 tattgaaaaa agcatattat aaacatgatg ttcatataga agttatcgat caacaaaata
     2101 aaattgaatt attgcctaaa ggtcaacata cagttgaaaa agataagagt tatccgtaca
     2161 tttcatttat accgatgact gatgatgtag aactttctct agcaggtttt aaatataatt
     2221 tagctagaca aatgcttaat ataggttcta ctttaactat ttcaaatgaa attgagtctt
     2281 tgcaagcgaa agtaactgta catgatgggt tgattttgca aattagaagt acagatttaa
     2341 attaagtata ttcaaatttg tgaggatttt taataaagtg catgtgttat ttaataaagt
     2401 gacgtttata gatagaattt ataacataat aatagatgaa tcacgctgta attaatgaag
     2461 attatggcgt gatttattat tggaaggaaa attgtaggtc ctagtacatt gatttatgtg
     2521 ccaaaatcga tattgaaccc ataagtccaa tttgacagag aaccattatt aaacgtatta
     2581 accaaattat attgatagtt cgaatttcac tatgaaccat tatgtattgt ttggtaacaa
     2641 aaaaagaact aagaggttga gctcttagtt cttaagtaaa tgtgtcctcc acataaggtc
     2701 acatttttta tgtgcaatat atttattaaa ctctagttac tttaccagat tttaaagcac
     2761 gtgcagaaac ccaaactttt ttaggtttac cgtcaactag gattctaact ttttgaaggt
     2821 tagcgttcca tctacgttta gtagagttta aagcgtgtga acgtctgtta ccagtcgaag
     2881 ctttacgacc tgttacgaaa cattgtttac ccatatgagt acctccttta ataaatataa
     2941 atacacataa ctacttatat acttaattaa gatagcatag tttcatttga aaaacaatga
     3001 ataattttca cataaaagtc aaaaatactg agttttgtga tataattgta gactgtgaag
     3061 ttatgtagta tgatatttta gagaaataag tgaatgaaac atttaaaatt tttatttaat
     3121 gatactacat ctattaaaag aaaacgccta tagataatcg tcatgagtgt aaattcagtt
     3181 tactcaaaat ataataggtg atttaagcgt aagtcattct gataagttgt accattgata
     3241 ttttataaaa ataatcaatg aaacttaaag cgtttatgac tacactaagt ggtaaaattt
     3301 taaaagtgat tagggaaaac gaataataaa ctttgaaatt taggagggac aaagatatga
     3361 cattagagat ttcaaatgat tacggtaaaa ttgatatttc aaacgaagtg attgcttcgg
     3421 ttgtaggtgg aaaggccgtt gaatgttatg gtattgtagg tatggcatct agacaacaag
     3481 ttagagatgg tattgcggaa atactaggac atgaaaatta tgctaaaggc atcaaagtaa
     3541 ctgaaaataa tggcgtagtg gatatagata tgtacattat tgtaagttat ggtgtgaaaa
     3601 tatctgaagt tgccaataat gtacaatcaa cagtgaaata tactttggaa aaatcactta
     3661 atgtatcagt aaattcaatc aatatatatg tacaaggtgt acgtgtgaat aatacaggca
     3721 agaaagctta ggaggacaac ttgaaatgat tagcaaaatt aatggtaaat tatttgccga
     3781 tatgattata caaggggcac aaaatttatc taacaatgca gatttggtag attctttgaa
     3841 tgtgtatcca gtgccagatg gtgatacagg aacaaatatg aatcttacta tgacttcagg
     3901 tcgcgaagaa gtagagaata atttgtcgaa aaatatcggc gaattaggta aaacattctc
     3961 gaaaggttta ctaatgggtg caagaggtaa ctctggtgtc atcttgtcac aattattcag
     4021 aggattttgt aaaaatattg aaagtgaatc tgaaattaat tcaaaattgt tagctgaaag
     4081 ttttcaagct ggtgttgaaa cggcatataa agctgttatg aaaccagttg aaggtacaat
     4141 acttacagtt gcaaaagatg ctgcgcaagc tgcaatagaa aaagcaaata atactgaaga
     4201 ttgtatagaa ttaatggagt acattattgt aaaagccaat gaatcacttg aaaacacacc
     4261 aaacttatta gctgtactta aagaagttgg tgttgttgat agtggcggta aaggtttgtt
     4321 atgcgtttac gaaggattct taaaagcgct taaaggtgaa aaagttgaag ccaaagttgc
     4381 aaagatagat aaagatgaat ttgtacatga tgaacatgat ttccatggtg taattaatac
     4441 tgaagatatt atttatggct attgtactga aatgatggtt cgttttggaa agaataaaaa
     4501 agcctttgat gaacaagaat tcaggcaaga tatgagtcaa tttggtgatt ctttattagt
     4561 cattaatgat gaagaaattg tgaaagttca cgtgcatacc gaatacccag gtaaagtgtt
     4621 taattatggt caacaatatg gtgaattaat taaacttaag gttgaaaata tgagagaaca
     4681 gcaccgtgaa gtgattcgaa aagaacagca cacagctaaa ccgaaaatgg aaacggttga
     4741 aacagcaatt attactattt ctatgggtga aggtatttca gagatattca aatcaatggg
     4801 tgccacacat atcattagtg gtggacaaac gatgaatcct tctacagaag atatcgttaa
     4861 agtcattgaa caatcaaaat gtaaacgtgc aattatttta ccgaataata aaaatatttt
     4921 aatggcaagt gaacaagcag cgagtattgt tgatgcagaa gcagttgtta ttccaacgaa
     4981 atctattcct caaggtataa gcgcactatt ccaatatgat gtggacgcaa cacttgaaga
     5041 aaataaagtg caaatggctg attcagtaaa taacgttaaa tctggttcat taacgtacgc
     5101 tgttcgtgat acgaaaattg atggcgttga gattaaaaaa gacgcgttta tgggcttgat
     5161 tgaagataag attgtaagca gccaaagtga tcaattaaca acggttactg agttgttaaa
     5221 tgagatgtta gcagaagata gtgaaatatt gactgtgatt attggtcaag atgcagagca
     5281 agcagttaca gataacatga taaactggat cgaagagcaa tatccagatg tagaagtgga
     5341 agttcatgaa ggtggacaac caatttatca atatttcttt tcagtagaat aaaaatttaa
     5401 aataaaaaac taccaatgat aaatcatcag ttggtagttt tttattttgc tattttagtg
     5461 ttattgcggg ttaaaagtat cattctcgag ttgctaacaa tgtcatgttc aacttagtca
     5521 tgataaaata aataacatac taaatgatac gtaaaatcaa ataaaacata ggtgatttat
     5581 tttggctaaa gtaaacttaa tagaaagtcc atattctctt ttacaattaa aaggtatagg
     5641 tcctaagaaa atagaagtat tgcaacaact aaatattcat acagtggaag atcttgttct
     5701 ttatttgcca actagatatg aagataatac agtgattgat ttgaatcaag cagaagatca
     5761 atctaacgtt acgatagaag gacaagtata tacagctcca gtagttgcat tttttggaag
     5821 aaataaatca aaattaaccg ttcatttaat ggtaaataat attgctgtca aatgtatttt
     5881 tttcaatcaa ccgtatttaa aaaagaaaat cgaattaaat caaactataa ctgttaaagg
     5941 taagtggaat agagttaaac aggaaattac tggtaatagg gttttcttta attcacaagg
     6001 gacacaaact caagaaaacg cagatgttca attagaacca gtctatcgta ttaaggaagg
     6061 tattaaacaa aagcaaatac gagaccaaat tagacaagcg ttaaatgatg tgacaattca
     6121 tgaatggtta actgatgaac taagagaaaa atataaatta gagaccttgg actttacttt
     6181 gaacacatta catcatccta aaagtaaaga ggatttatta cgtgctcgta gaacctatgc
     6241 atttactgaa ctgtttttat tcgaattacg tatgcaatgg ctaaatagat tagaaaagtc
     6301 atctgacgaa gcaattgaaa ttgattatga cttagaccaa gttaaatcat ttattgatcg
     6361 tttacctttt gaactaactg aagcacagaa atccagtgtt aatgaaattt ttagagattt
     6421 aaaagcacca atacgtatgc atcgattact tcaaggtgat gtaggttcag gaaaaacagt
     6481 agttgctgca atttgtatgt atgcgttaaa aacggctggt tatcaatcag cattgatggt
     6541 accaactgaa attttagcag agcaacatgc tgaaagttta atggctttat ttggagattc
     6601 tatgaacgtt gcattgttaa ctgggtcagt aaaaggtaag aaacgaaaga tacttttaga
     6661 acaacttgaa aatggtacga ttgattgttt aattggaacc catgctttga ttcaagatga
     6721 tgtgattttc cataatgttg gtttagtaat tacagatgaa caacatcgat ttggtgtgaa
     6781 tcaacgccag cttttaagag aaaaaggtgc aatgacgaat gtgttattta tgacagcaac
     6841 gccgatacca agaacactag caatatcagt ttttggtgag atggatgtgt cttcaattaa
     6901 acaattacca aaaggtcgta aacctatcat tactacttgg gcaaagcatg agcaatacga
     6961 taaagttttg atgcaaatga cctcagagtt gaaaaaaggt cgtcaagcat atgtcatttg
     7021 cccgctaata gaaagttctg agcatctcga agatgttcaa aatgttgtcg cattgtacga
     7081 gtctttacaa cagtattatg gtgtttcccg tgtagggtta ttgcatggta agttatctgc
     7141 cgatgaaaaa gatgaggtca tgcaaaagtt tagtaatcat gagatagatg ttttagtttc
     7201 tactactgtt gttgaagtag gtgttaatgt accgaatgca acttttatga tgatttatga
     7261 tgcggatcgc tttggattat caactttaca tcagttacgc ggtcgtgtag gtagaagtga
     7321 ccagcaaagt tactgtgttt taattgcatc ccctaaaaca gaaacaggaa ttgaaagaat
     7381 gacaattatg acacaaacaa cggatggatt tgaattgagt gaacgagact tagaaatgcg
     7441 tggtcctgga gatttctttg gtgttaaaca aagtgggttg ccagatttct tagttgccaa
     7501 tttagttgaa gattatcgta tgttagaagt tgctcgtgat gaagcagctg aacttattca
     7561 atctggcgta ttctttgaaa atacgtatca acatttacgt cattttgttg aagaaaattt
     7621 attacatcgc agttttgact aattaccatg ctgatttgtc aatttgagtg caacacttcg
     7681 ttaattgagt gatatgacac ttgaactatt taaatgtaaa gtggtatttt aacaatttat
     7741 aaatttccga ctaaataata gctaaataat acagttattt gttgagtcgg ttaaatagaa
     7801 agtgttatga tatgtgagga atgtttaaga ctaggtacta aaaaatgagg ggtgagacgt
     7861 tgaaactaaa gaaagataaa cgtagagaag caatcagaca acaaattgat agcaatccct
     7921 tcatcacaga ccatgaacta agcgacttat ttcaagtgag tatacaaaca attcgtttag
     7981 atcgcactta tttaaacata ccagaattaa ggaagcgtat taaattagtt gctgaaaaga
     8041 attatgacca aataagttct attgaagaac aagaatttat tggtgatttg attcaagtca
     8101 atccaaatgt taaagcgcaa tcaattttag atattacatc ggattctgtt tttcataaaa
     8161 ctggaattgc gcgtggtcat gtgctgtttg ctcaggcaaa ttcgttatgt gttgcgctaa
     8221 ttaagcaacc aacagtttta actcatgaga gtagcattca atttattgaa aaagtaaaat
     8281 taaatgatac ggtaagagca gaagcacgag ttgtaaatca aactgcaaaa cattattacg
     8341 tcgaagtaaa gtcatatgtt aaacatacat tagttttcaa aggaaatttt aaaatgtttt
     8401 atgataagcg aggataaaat tatggttaaa ttagcaattg atatgatggg tggcgacaat
     8461 gcgcctgata tcgtattaga agccgtacaa aaggctgttg aagactttaa aaatctagaa
     8521 attatgcttt tcggtgacga aaaaaagtat aatctgaacc atgaacgaat cgaatttaga
     8581 cattgttctg aaaagattga aatggaagat gagcctgtta gagcgattaa acgtaaaaaa
     8641 gatagctcaa tggtaaaaat ggctgaagct gtgaaatctg gtgaagcaga tggatgtgtg
     8701 tcagcaggta atactggtgc tttaatgtca gttggtttat tcattgttgg acgtattaaa
     8761 ggtgtagcta gaccggcttt agtagtaaca ttgccaacga ttgatggaaa aggttttgtc
     8821 tttttagacg ttggtgcaaa tgctgatgct aaacctgaac acttattaca gtatgcgcaa
     8881 ctaggggata tttatgctca aaaaattaga ggtattgata atccgaaaat ctcattatta
     8941 aatataggta ccgagccagc taaaggtaat agtttaacga aaaaatcatt tgagttatta
     9001 aatcaagatc attcattgaa ttttgttggg aatattgaag cgaagacatt aatggatggc
     9061 gatacagatg ttgtagttac cgatggctat actgggaaca tggtccttaa aaatttagaa
     9121 ggtactgcaa aatcaatcgg taaaatgtta aaagatacga ttatgagtag tactaaaaat
     9181 aaattagcag gtgcaatatt gaagaaagat ttagcagaat tcgctaaaaa gatggattac
     9241 tcagaatacg gtggttcagt attattagga ttggaaggta ctgtagttaa agcacacggt
     9301 agttcaaatg ctaaagcttt ttattctgca attagacaag cgaaaatcgc aggagaacaa
     9361 aatattgtac aaacaatgaa agagactgta ggtgaatcaa atgagtaaaa cagcaattat
     9421 ttttccggga caaggtgccc aaaaagttgg tatggcacaa gatttgttta ataacaatga
     9481 tcaagcaact gaaattttaa cttcagcagc aaagacgtta gactttgata ttttagagac
     9541 aatgtttact gatgaagaag gtaaattggg tgaaactgaa aacacgcaac cagctttatt
     9601 gacgcatagt tcggcattat tagcagcgct aaaaaatttg aatcctgatt ttactatggg
     9661 gcatagttta ggtgaatatt caagtttagt tgcagctgac gtattatcat ttgaagatgc
     9721 agttaaaatt gttagaaaac gtggtcaatt aatggcgcaa gcatttccta ctggtgtagg
     9781 aagcatggct gcagtattgg gattagattt tgataaagtc gatgaaattt gtaagtcatt
     9841 atcatctgat gacaaaataa ttgaaccagc aaacattaat tgcccaggtc aaattgttgt
     9901 ttcaggtcac aaagctttaa ttgatgagct agtagaaaaa ggtaaatcat taggtgcaaa
     9961 acgtgtcatg cctttagcag tatctggacc attccattca tcgctaatga aagtgattga
    10021 agaagatttt tcaagttaca ttaatcaatt tgaatggcgt gatgctaagt ttcctgtagt
    10081 tcaaaatgta aatgcgcaag gtgaaactga caaagaagta attaaatcta atatggtcaa
    10141 gcaattatat tcaccagtac aattcattaa ctcaacagaa tggctaatag accaaggtgt
    10201 tgatcatttt attgaaattg gtcctggaaa agttttatct ggcttaatta aaaaaataaa
    10261 tagagatgtt aagttaacat caattcaaac tttagaagat gtgaaaggat ggaatgaaaa
    10321 tgactaagag tgctttagta acaggtgcat caagaggaat tggacgtagt attgcgttac
    10381 aattagcaga agaaggatat aatgtagcag taaactatgc aggcagcaaa gagaaagctg
    10441 aagcagtagt cgaagaaatc aaagctaaag gtgttgacag ttttgcgatt caagcaaatg
    10501 ttgccgatgc tgatgaagtt aaagcaatga ttaaagaagt agttagccaa tttggttctt
    10561 tagatgtttt agtaaataat gcaggtatta ctcgcgataa tttattaatg cgtatgaaag
    10621 aacaagagtg ggatgatgtt attgacacaa acttaaaagg tgtatttaac tgtatccaaa
    10681 aagcaacacc acaaatgtta agacaacgta gtggtgctat catcaattta tcaagtgttg
    10741 ttggagcagt aggtaatccg ggacaagcaa actatgttgc aacaaaagca ggtgttattg
    10801 gtttaactaa atctgcggcg cgtgaattag catctcgtgg tatcactgta aatgcagttg
    10861 cacctggttt tattgtttct gatatgacag atgctttaag tgatgagctt aaagaacaaa
    10921 tgttgactca aattccgtta gcacgttttg gtcaagacac agatattgct aatacagtag
    10981 cgttcttagc atcagacaaa gcaaaatata ttacaggtca aacaatccat gtaaatggtg
    11041 gaatgtacat gtaatatatt tgagctaaag ctcattgacg cagtggttga ctggtcatcc
    11101 aatggagaat tgtctgacct agtcaacttt gcgggggaaa ttctaagcaa cctagataag
    11161 gttccagaat ttctccctaa gaaacactaa tcaataaatt gataagaaga ttagaggaac
    11221 gcttgagcta aagctcaaga taaagacgca gaaatcaata aattgataac tgggatagag
    11281 gtacgcctga gctaaagctc aagataaaga cgcagtaatc aataaattga taactgggat
    11341 agaggtacac ctgagctaaa gctcatg
//
LOCUS       DAOBBH010000075        11286 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.075, whole genome shotgun sequence.
ACCESSION   DAOBBH010000075 DAOBBH010000000
VERSION     DAOBBH010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11286
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.075"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(145..2121)
                     /gene="sfaD"
                     /locus_tag="P6L01_001431"
                     /gene_synonym="sfnaD"
     CDS             complement(145..2121)
                     /gene="sfaD"
                     /locus_tag="P6L01_001431"
                     /gene_synonym="sfnaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine--citrate ligase SfaD"
                     /protein_id="HDP3030503.1"
                     /translation="MNLNLIFKEQTLKFNKEEQETYLFLQQHNSDWANIFKEMILQGR
                     DKVTQRLVTSMHRENLVKARTQSKKILSRDLIMLDISTTHILEIQFPQAKQTLYAPIT
                     GEHAFDRIDVEGPFYIKDDITNTITRVHHPNEILECILIEAPDLKNAASDQFQQDLIN
                     SATNMTFAISYQALSMQHDSAPLFNIIENSEDSYLRSEQAVIEGHPLHPGAKLRKGLN
                     ALQTFLYSSEFNQPIKLKIVLIHSKLSRTMSLSKDYDTTVHQLFPDLIKQLENEFTPN
                     FNFNDYHIMIVHPWQLDDVLHSDYQAEVDKELIIEAKHTLDYYAGLSFRTLVPKYPAM
                     SPHIKLSTNVHITGEIRTLSEQTTHNGPLMTRILNDILEKDVIFKSYASTIIDEVAGI
                     HFYNEQDEVDYQTERSEQLGTLFRKNIYQMIPQEVTPMIPSSLVATYPFNNESPIVTL
                     IKRYQSAASLSDFESSAKSWIETYSKALLGLVIPLVTKYGIALEAHLQNAIATFRKDG
                     LLDTMYIRDFEGLRIDKAQLNEMGYSTSHFHEKSRILTDSKTSVFNKAFYSTVQNHLG
                     ELILTISKASNDSNLERHMWYIVRDVLDNIFDQLVLSTHKSNQVNENRINEIKDTMFA
                     PFIDYKCVTTMRLEDEAHHYTYIKVNNPLYRENN"
     gene            2223..3416
                     /gene="sfaA"
                     /locus_tag="P6L01_001432"
     CDS             2223..3416
                     /gene="sfaA"
                     /locus_tag="P6L01_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A export MFS transporter"
                     /protein_id="HDP3030504.1"
                     /translation="MTKYFFSSSFLLFLGNWIGQIGLNWFVLTTYHNAVYLGIVNFCR
                     LVPILLLSVWAGAIADKYDKGRLLRITISSSFLVTAILCVLTYSFTAIPISVIIIYAT
                     LRGILSAVETPLRQAILPDLSDKISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAP
                     TTFLAQAICYFIAALLCLPLHFKVTKIPEDATRYMPLKVIIDYFKLHMEGRQIFITSL
                     LIMATGFSYTTLLPVLTNKVFPGKSEIFGIAMTMCAIGGIIATLVLPKVLKYIGMVNM
                     YYLSSLLFGIALLGVVFHNIVIMFICITLIGLFSQWARTTNRVYFQNNVKDYERGKVL
                     SIIMMDRGMIPLGSLLMSICADVFGIVRTFSIMGISTICITMVFYFINRKLKLKLEES
                     NHGIS"
     gene            3403..5160
                     /gene="sfaB"
                     /locus_tag="P6L01_001433"
     CDS             3403..5160
                     /gene="sfaB"
                     /locus_tag="P6L01_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A synthetase SfaB"
                     /protein_id="HDP3030505.1"
                     /translation="MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKGSLIEIQY
                     HMHVLTIEVVRKSALERYEFTGDITYLNKGETSLIITLEGLLDVLNHDFDIPISERLR
                     EELIHSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYS
                     ESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAMDGNDQ
                     FIIDEIIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPT
                     PFTILSKATLSFRTMSLIDKPYHVKLPVDAQATSAVRTVSTVTTVDGPKLSYALQNML
                     NQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQK
                     VIVDSYLEWLNQGITKESITTFIERYAQALIPPLIAFIQNYGIALEAHMQNTVVNLGP
                     HFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAE
                     LIHHFNQYDCVEETELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRME
                     NKVKQYLNIELDNPIKKEV"
     gene            5167..6237
                     /gene="sfaC"
                     /locus_tag="P6L01_001434"
     CDS             5167..6237
                     /gene="sfaC"
                     /locus_tag="P6L01_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A biosynthesis protein SfaC"
                     /protein_id="HDP3030506.1"
                     /translation="MAHVNINISKIKYNAKVLQTVFQSKNIQFTPVIKCIAGDRTIVE
                     SLKALGINHVAESRLDNIISIADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIH
                     QINEVAEVLGKKHKILLMVDWKDGREGVLTYDVLDYIKEIIHLKNIHFVGLAFNFMCF
                     KSDAPSDDDIFMINRFVSAVEREIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGET
                     LFRGVDTTTNQAIAMLYQDAITLEAEILEIKPRVNTQTHESFLQAIVDIGYLDTKVDN
                     ISPMDQHINILGASSDHLMLDLNGQGHYQVGDHISFSLNYEALSHSMYMKNLHKVYID
                     DSKIDTLLQNFDVKSPAMVNQY"
     gene            complement(6338..6463)
                     /locus_tag="P6L01_001435"
     CDS             complement(6338..6463)
                     /locus_tag="P6L01_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030507.1"
                     /translation="MYHCYIIFALIQLTPFSMTVIPVIQLILSVAILCDELLHLI"
     gene            6629..7612
                     /locus_tag="P6L01_001436"
     CDS             6629..7612
                     /locus_tag="P6L01_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDP3030508.1"
                     /translation="MRGLKTFSILGLIVALLLVAACGNTDNSSKKESSTKDTISVKDE
                     NGTVKVPKDAKRIVVLEYSFADALAALDVKPVGIADDGKKKRIIKPVREKIGDYTSVG
                     TRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNINSFKTI
                     AKALNKEKEGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQF
                     LNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTGHAKKDSAEFKK
                     LQEDATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKELVELSKKEQK"
     gene            7624..8655
                     /locus_tag="P6L01_001437"
     CDS             7624..8655
                     /locus_tag="P6L01_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDP3030509.1"
                     /translation="MAIKEISSQSAIDHKRKRRTTLTYIVSLCFLFICIYLNMAIGSS
                     KINFSDIIHYVTGHTDTKATFLLHNVRMPRMIAGLFIGGALAVSGLLMQAMTRNPLAS
                     PKIFGVSSGASFFIVFVTIIIPSLEYYALYLGVIGAFIGGLTVYTLSGATKGMTPIKL
                     ALAGMAIHLFFSSMTEGIIILNENSNEQVMFWLVGSLSSMKWDEILTILPWIIGALIV
                     TIFIGRQLTIMELGDDIAKGLGQNINKVRIIIGLLVIILTGMSVSVAGPIGFVGLIVP
                     HIVKRYVSKNYLVMIPLTFIIGADLLLLSDVLSRLITYPYESPVGIVTSFVGALYFLF
                     ITIKGVKRI"
     gene            8652..9620
                     /locus_tag="P6L01_001438"
     CDS             8652..9620
                     /locus_tag="P6L01_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDP3030510.1"
                     /translation="MMTTKIIRRYSLITILLIVSIFISLCVGSVMIHPIDAIKGIFTQ
                     NDFILNEYRIPRTLLGIIIGSSLAISGAIIQAVIRNPLASPDVIGISKGASLAAVIII
                     MIFPTAPLFVLPIGSFLGAFAVSLFLSFLISKYDVKGSKLALIGLAIGAICTAIVQFL
                     LIRNPLEANKALVWLTGSLYGHDMSNIYTILPWFLITIPIIILLSYQLDVLNLGDAVA
                     TALGLKVKTIKMLLLILAVILAGSAISVVGGISFLGLIAPHIARQLIGNKTLHVIIMS
                     GLIGAILLTFGDGLARGIHPPLDIPVGVIIAIIGAPYFLILLRRMK"
     gene            9856..11226
                     /locus_tag="P6L01_001439"
     CDS             9856..11226
                     /locus_tag="P6L01_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HDP3030511.1"
                     /translation="MDSFSRKEIVIGRLKFITMSLIGILLFLVPIPVEQDGQKQTTLP
                     VAFLAGVLKDVLGGVMPFLIVTIITLSGIITLICSTILKDKLKPDGLMNNAFNVRIGW
                     LILRILAVVFAWMTFLRIGSKVIYSDETGGLLFSSLLPTLVAVFLFAALFLPLLMEYG
                     LLEMLGPIFRPVMRPLFTLPGRSTVDNLASFIGDGTVGVLITSRQYGEGYYSRREATV
                     ISTTFSVVSITFAIVVAETVHMQNQFFAFYLSVIVSCLVAAVIMPRIWPLNKIPDEYA
                     KEVPESARTEALPEGKTALRHGFDTATEVGIKAPGVIDFFKSGLKTVIDMWFVILPVV
                     MSIGTIATIIANYTPFFVILGKPFVPFLELMQIPEAAQASQTIIIGFADMFLPSILIE
                     GVQNDITRFVIGALSISQLIYLSEVGGVILGSKIPVSIGKLFMIFLIRTIITLPIISL
                     MAHLLL"
BASE COUNT         3740 a         1578 c         2065 g         3903 t
ORIGIN      
        1 gctcggcaca ccattttagt gccgagcttt tttgtgattt ttaataattt gaatatggaa
       61 aatgtattat tctctcattt gtatagattg tatttaataa gttaatgtaa tccttgagct
      121 cacgattaat aaaattctat aattttaatt attttctcga tacaaaggat tattaacttt
      181 aatataagta taatgatgtg cctcatcttc aagacgcatt gttgtaacac acttataatc
      241 tataaatggc gcgaacatgg tatctttaat ttcatttatg cgattctcat ttacttgatt
      301 agatttgtgt gtcgaaagta caagttgatc aaaaatgtta tctagtacat cacgaacgat
      361 ataccacata tgtctttcta agtttgaatc atttgatgct ttagagattg taagaattaa
      421 ttcgcctaaa tggttttgaa cggtagaata aaaggctttg ttaaacacag acgtttttga
      481 atcagtaaga attcttgatt tttcatggaa atgagatgta ctgtatccca tttcatttag
      541 ttgtgcttta tcaattctta aaccttcaaa gtctctaata tacatcgtgt ccaatagacc
      601 atctttccga aatgttgcaa tagcattttg caagtgagcc tctaatgcaa tgccatattt
      661 agtaactagt gggattacga gaccaagcaa tgctttacta taagtttcaa tccacgattt
      721 cgccgatgat tcaaaatcag acaatgatgc agctgattga taacgtttaa tcaatgtcac
      781 gataggtgat tcattgttaa atgggtaggt tgcaactaag cttgaaggaa tcattggtgt
      841 gacttcttgt ggaatcattt ggtatatgtt ttttctaaat aatgtaccta attgttcact
      901 tcgttcagtt tgatagtcta cttcgtcttg ctcattataa aaatgaatac cagcaacttc
      961 atcaataatt gttgatgcat aggacttaaa tatgacatct ttctccaaaa tatcatttaa
     1021 aatacgtgtc attagtggac cattgtgcgt cgtttgttct gataatgtac gaatttcacc
     1081 tgtaatatga acgtttgtcg acaatttgat gtgtggcgac atagctgggt atttaggaac
     1141 taatgttctg aaagataaac cggcataata atccaacgta tgttttgctt caatgattaa
     1201 ttctttatct acttctgctt gataatcaga atgtaatacg tcatctaatt gccatggatg
     1261 tacaatcata atgtgataat cattaaagtt aaagtttggc gtaaattcat tttctaattg
     1321 tttaattaag tccggaaata gctgatgaac agttgtatca taatctttag acagtgacat
     1381 agtacgactt aatttactgt gaatcagtac tattttcaac ttaataggtt gattaaattc
     1441 tgaagaatat aggaatgttt gtaatgcatt taaaccttta cgtaatttag ccccaggatg
     1501 tagcggatga ccttcaataa cggcttgctc tgaacgcaag taactatctt cgctattttc
     1561 gataatatta aataaaggtg cagaatcatg ttgcattgac agtgcttgat agctaattgc
     1621 aaatgtcata ttagttgcac tgtttattaa atcttgctga aattgatcac tagcagcatt
     1681 ttttaaatct ggtgcttcaa ttaaaataca ctcaagaatt tcatttggat ggtgtactcg
     1741 tgtaatcgta tttgtaatgt catctttaat gtagaaaggg ccttcaacat caattcgatc
     1801 aaaggcgtgt tctccagtga taggagcata taatgtttgc ttagcttgtg ggaactggat
     1861 ttctagtata tgagtcgttg agatatctaa cataatcaaa tcacgactca atattttctt
     1921 actttgagtg cgcgctttaa ctaagttttc gcgatgcatt gatgtgacta atctctgagt
     1981 gactttatct cttccttgta aaatcatctc tttaaaaata ttagcccaat cgctattatg
     2041 ttgttgtaaa aataaatatg tttcttgttc ttctttgtta aattttaatg tctgttcttt
     2101 aaaaattaag ttcaagttca taattcacct ctatgaaata ttttacaaaa gcaagataga
     2161 tttgtataat ccatattaat gataatgatt cttattatca acagaatgcg ggtgtaagtt
     2221 ttatgacaaa atattttttt agcagttctt ttctactatt tctaggtaat tggattggac
     2281 aaatagggct aaattggttt gtacttacca cttatcataa cgcagtttat ctggggattg
     2341 tcaatttttg cagacttgta ccaatattat tactaagtgt gtgggcaggg gcaattgccg
     2401 ataaatatga taaagggcga ttgctgagaa ttacaatttc atcatcattt ttagtaactg
     2461 caattttatg tgtgctcacg tatagtttca ctgcaattcc aattagcgtc attattatat
     2521 atgcgacatt aagagggatt ttaagtgcgg ttgaaacacc tttaagacaa gcaatcttac
     2581 cagatttatc agataaaata tctactacac aagctgtatc atttcattca ttcatcatta
     2641 atatttgtcg ttcaataggg cctgccattg ctggtgtcat attagcagtc tatcatgcgc
     2701 caacaacatt tcttgcacag gcaatttgtt attttatcgc agctttatta tgcttaccat
     2761 tacattttaa agtaactaaa atacctgaag atgcaacaag atacatgccg ttaaaagtta
     2821 ttatagatta cttcaaatta catatggaag gtcgacaaat atttataaca tcattattga
     2881 ttatggcgac aggtttttca tatacgacac ttttaccagt tttgacaaac aaagtatttc
     2941 cggggaaatc tgaaatattt ggtatcgcta tgacgatgtg tgccattggt ggtattattg
     3001 caacgctagt tttacctaaa gtacttaaat atattggtat ggtaaatatg tattatttaa
     3061 gttcactttt atttggcatt gctttgttag gtgtggtatt tcacaatatt gtcatcatgt
     3121 tcatttgtat tacattgatt gggttattta gtcaatgggc acgtacgaca aatcgcgttt
     3181 attttcaaaa taatgttaaa gattatgaac gtggtaaagt actgagtatt attatgatgg
     3241 atagaggtat gattccattg ggaagtctgt taatgagtat atgtgcagat gtgtttggca
     3301 ttgttagaac tttttcaata atgggaataa gtactatatg cattacaatg gtattctatt
     3361 ttataaatag aaagttgaag ttaaagttgg aggaaagtaa tcatggtata tcttgaatgg
     3421 gcaaaggcag atagaaatat tcaatatcgt gtaattaacg ccattattaa agaacgtatt
     3481 taccccgagc aaacatttat ttcgcaaaaa ggatctttaa ttgaaattca gtatcatatg
     3541 catgtgttga ctattgaagt tgttagaaaa agtgcattag aacgctatga gtttacaggt
     3601 gatattactt atttaaataa aggtgaaacg tcattaatta taactttaga aggtttgtta
     3661 gatgtgttga atcatgactt tgatatccct atttcagagc gactacgcga agagttaata
     3721 cacagtcgag atagtttagt tgaaacatat aagcaaatgt ctcacagaca aacattaata
     3781 agtcaaagtt ttaaattttc aagattacca caagatatta actttttttc atggttacaa
     3841 catgtaaaag atagtgataa gacagatgat ttaacttatt ctgagagctt ggtaccagag
     3901 gggcatccaa cacacccttt aaccaaaacg aaattgccct taactatgga agaagtacga
     3961 gcatatgcac ctgagtttga aaaagaaatc cctttgcaaa ttatgatgat tgaaaaagat
     4021 catgttgtgt gcacagctat ggatggtaat gatcaattta ttattgatga aataattccc
     4081 gaatactaca atcagattcg tgtgttttta aagagtttag gtttgaaaag tgaagactat
     4141 agagcgattt tagtacatcc ttggcaatat gatcatacga tagggaaata ttttgaagca
     4201 tggattgcta aaaaaatatt aattccaacg ccgtttacaa tactttcaaa agcaacttta
     4261 tcatttagga cgatgtcttt aattgataaa ccataccatg ttaagttgcc cgtcgatgca
     4321 caagcaacaa gtgccgttag aacagtctca actgtgacta ctgtagatgg accaaagtta
     4381 agttatgctt tacaaaacat gttgaatcaa tatccaggat ttaaagttgc tatggaaccg
     4441 ttcggtgaat atgcaaatgt tgataaagat agggcacgtc agcttgcttg tattataaga
     4501 caaaagcctg aaattgatgg aaaaggtgca acagtagtta gtgcaagtct agttaataaa
     4561 aatccaatag atcaaaaagt tatcgtggat agttacttag agtggttaaa tcaaggaatt
     4621 actaaagaaa gtattacgac atttattgaa cgatacgctc aagcattaat cccgccttta
     4681 attgctttta ttcaaaatta tggaattgca ttagaagcac acatgcaaaa tacagtagtg
     4741 aacttggggc cacattttga cattcaattt ttagtgagag atttaggtgg ttctagaatt
     4801 gatttagaaa cattacaaca tcgtgtatca gatattaaaa ttacaaatga tagtttaata
     4861 gctgattcta tagatgcagt gattgcaaaa ttccaacatg ctgttattca aaatcaaatg
     4921 gcagaattaa tccatcattt taatcagtat gattgtgttg aagaaaccga attatttaac
     4981 atagtacagc aagtagtagc gcatgctatt aacccaacac taccacatgc aaatgagtta
     5041 aaagatattt tgtttggacc aacaattact gtcaaagcgt tgttaaatat gagaatggaa
     5101 aataaagtaa agcaatattt aaatattgag ttagataatc cgataaaaaa agaggtgtag
     5161 tactacatgg cacacgttaa cataaatata tcgaagatta aatataacgc caaagtactt
     5221 caaacagttt ttcaaagtaa aaatatacaa ttcacaccag taattaagtg tatagctggt
     5281 gaccgtacaa ttgtagaaag cttaaaagcg ttaggtatca atcatgttgc agaatccaga
     5341 ttggataaca taattagtat tgcagatcag gatttaacat atacgttatt gcgtacgcct
     5401 gccaaaaaag agatttcaga tatgatagaa aaagttgata tgagtatcca aacagaatta
     5461 tctaccatac atcaaataaa tgaagtagca gaagttttag gaaaaaaaca taagatttta
     5521 ttaatggtag attggaaaga tggccgtgaa ggtgttctga cttatgatgt tttagattat
     5581 attaaagaga ttattcactt gaaaaatatc cattttgtag gtttagcatt caattttatg
     5641 tgttttaaat ctgatgcgcc aagtgatgat gatatcttta tgattaatcg attcgtctca
     5701 gctgttgaaa gagaaatagg atatcgattg aaaattattt caggtggtaa ttcgagcatg
     5761 ttaccacaac ttttatataa tgatttgggt aaaataaatg aattgcgaat aggtgaaacg
     5821 ttgtttagag gtgttgatac aacgacaaat caagcgatcg ctatgttata tcaagatgcg
     5881 attacattgg aggctgagat tttagaaatt aaacctcgag ttaatacaca aacgcatgaa
     5941 tcattcttac aagctattgt agatattggt tatttagata caaaagtcga taatatttca
     6001 cctatggatc agcatatcaa tattttaggc gcttcgagtg atcatttaat gcttgattta
     6061 aatggtcaag gacactatca agttggagat catatatctt tttcattaaa ttatgaagca
     6121 ctttctcatt cgatgtatat gaaaaattta cataaggtat atattgatga ttcaaagatt
     6181 gatacgctac ttcaaaattt tgatgtgaaa tctcctgcta tggtaaacca atattaatat
     6241 atttatcaat aattctaagt tgacaagtta tactacatat cgtttgcacg gttgtatcat
     6301 aattgttcaa cttagatttt ttgtatttgt tgatttatca aattaagtgc aacagttcgt
     6361 cacataaaat tgcaacagat aatatcagct gaattacagg gataacggtc atgctaaatg
     6421 gtgtcaattg tattaatgca aagatgatat agcaatgata cattatcagt attttgtcta
     6481 aggaaatgtg ctaattgtag tcataattat tagaggaaat atatagtcat acattttaga
     6541 aatataaaaa agattgaacg ctacttgaca atgataattg ttatcaataa aataataaat
     6601 gaagttatac atattaagga gtggaacgat gagaggtcta aaaactttta gtatattggg
     6661 attaatagtt gccttacttt tagttgcagc ttgtggtaat acggataatt caagtaaaaa
     6721 agaatcatca actaaagata ctatttcggt aaaagatgaa aatggtacag taaaagtacc
     6781 taaagatgca aaacgtatcg ttgtattaga gtactcattt gcagatgcat tagcagcatt
     6841 agacgttaaa ccagttggta ttgctgatga tggtaagaaa aaacgtatca ttaaaccagt
     6901 tagagaaaaa attggggatt atacttctgt aggtacacgt aaacagccaa acttagagga
     6961 aattagtaaa ttaaaaccgg atttaattat cgctgatagc agtagacata aaggtattaa
     7021 taaagaatta aacaaaattg caccaacatt atcattaaag agttttgatg gagactacaa
     7081 acaaaatatt aattcgttca aaacaattgc taaagcttta aataaagaaa aagaaggcga
     7141 aaaacgtctt gctgagcatg ataaattaat caataagtat aaagatgaaa ttaaatttga
     7201 tagaaatcaa aaagtgcttc cagcagtagt tgctaaagct ggtttattag cacatccaaa
     7261 ctattcatat gttggacaat ttttaaacga actaggattt aaaaatgcat taagtgacga
     7321 tgtaacaaaa ggtttaagta aatatttgaa aggaccttac ttacaattag acactgaaca
     7381 tttagctgat ttaaatccag agcgtatgat cattatgaca ggtcatgcta aaaaagattc
     7441 tgctgaattc aagaagttac aagaagatgc aacatggaaa aagttgaatg cagttaaaaa
     7501 taatcgcgtg gatattgttg accgtgatgt ttgggcaaga tctcgtggct taatttcttc
     7561 tgaagaaatg gctaaagaac ttgttgaatt atcaaaaaaa gaacaaaagt aaggtggaag
     7621 taaatggcta taaaagaaat aagtagccaa tctgccatag atcataaaag aaaaagacgc
     7681 acaacactca cgtatatagt gagtttgtgc tttcttttta tttgtatata tttaaatatg
     7741 gcgattggtt cttcgaaaat taattttagc gatatcattc actatgttac tggtcataca
     7801 gatacgaaag caacgttttt attgcataat gtacgtatgc caaggatgat tgcagggtta
     7861 tttattggcg gtgcattagc ggtatctggt ttgttaatgc aagcaatgac acgcaatccg
     7921 ctagcatcac ctaaaatttt tggtgtcagt tctggtgcat cgtttttcat tgtatttgtg
     7981 acgattatca ttccatcatt agaatattat gcattatatt taggtgtgat aggtgcattt
     8041 ataggtggtt taactgttta tacactttca ggtgctacga aaggtatgac acccattaaa
     8101 ttagcattgg ctggtatggc catccatttg ttttttagta gtatgactga aggtattatt
     8161 attttgaatg agaattcaaa cgaacaagtg atgttttggc ttgtcggttc attatccagt
     8221 atgaagtggg atgaaatttt aaccatttta ccatggatta ttggtgcttt aattgtaacg
     8281 atttttattg gacgtcaact aacgattatg gaactaggcg atgacattgc taagggattg
     8341 ggtcaaaata taaataaagt tagaattatc attgggctat tagtcattat tcttacaggt
     8401 atgtcggtgt cagtggcagg accaattgga tttgttggct taatcgtacc gcatatagtc
     8461 aaacgctatg tgagtaagaa ttatttagtc atgattccat taacatttat tataggtgca
     8521 gatttgttac tactatcaga tgtattaagt cgtttaatca cttatcctta cgaatcacct
     8581 gtaggtattg tgacatcatt cgtaggtgca ctttacttct tatttataac gattaaaggg
     8641 gtgaaaagaa tatgatgaca acgaaaatta tacgtcgtta tagtttaatc acaatccttt
     8701 taattgtgag tatattcata agtttatgtg tagggtctgt catgattcat ccgattgatg
     8761 ctattaaggg aatctttaca caaaatgatt ttattttaaa tgagtatcgt atacctagaa
     8821 cattacttgg tattattatc ggtagtagct tggctatatc aggtgcaatt atacaagctg
     8881 taataagaaa cccgttagcc tcacctgatg ttataggtat ttctaaaggt gccagtttag
     8941 ctgctgtaat tattattatg atctttccaa cagcaccatt gtttgtttta cctataggtt
     9001 cattcttagg tgcctttgca gtaagtttat ttttatcatt tttaatttca aaatatgatg
     9061 ttaaaggttc aaagcttgcc ttaattggtt tagcaatagg cgcaatatgt acggcgattg
     9121 tgcagttctt attaatcaga aatccactgg aagcaaacaa agcgcttgtt tggttgacag
     9181 gtagtctata tggacatgat atgtcgaata tttatactat attaccttgg tttttaataa
     9241 cgataccaat catcatttta ttgagttatc aattagatgt attgaatcta ggtgatgctg
     9301 ttgcaaccgc actcggttta aaagttaaaa ctattaaaat gctattgcta attttagcag
     9361 taatattagc aggatccgca atttcagttg tcggtggtat tagcttttta ggattaattg
     9421 cacctcatat tgcgagacaa ttaataggaa ataaaacatt acacgttatt atcatgtcgg
     9481 ggttaattgg tgctatattg ttaacatttg gagatggttt agcccgtggc attcatccgc
     9541 cactggatat tcctgtaggg gttatcattg caattatcgg tgcaccatac ttcttaatat
     9601 tattacgaag aatgaaataa ctaacatatg attagagttt aaaaaaggtt aagtacgaaa
     9661 tcagtaattg tacttaacct tttttgaatt catcttgaaa aattacaagt aatagcgtat
     9721 atttaataac tgtaattatt cattattttg catacataaa cattttgaaa gatagaatga
     9781 taaggaatat gtttgttaac tgaatagaat tctgtatata cttaaaacga atacatagag
     9841 aaatggattg attttatgga tagcttttca aggaaagaga tagttattgg gagacttaag
     9901 tttattacga tgtctttaat tggtatctta ttatttctag taccaattcc agtcgaacaa
     9961 gatggacaga agcaaacaac gttgcccgta gcatttttag caggcgtgct taaagatgta
    10021 ttaggtggcg taatgccatt tttaattgtg acgataatta ctttatcggg tatcataact
    10081 ctgatttgtt caactatttt aaaagataaa ttgaaacctg acgggctaat gaacaatgct
    10141 tttaacgtta gaattggttg gttaatttta cgaatattag cagttgtttt tgcatggatg
    10201 acatttttac gaattggttc aaaggttatt tactcagatg aaacgggtgg attattgttc
    10261 tcaagtttat tgccaacatt agtggcagta ttcttatttg cagctctatt tttaccgcta
    10321 ttaatggaat acggtttgtt agagatgttg ggtcctattt ttagaccggt tatgagacct
    10381 ttatttacat tgccaggacg ttcgacggta gataatttag catcatttat tggagacgga
    10441 actgttggtg tcttaattac aagtagacaa tatggtgaag gatattattc tcgtcgtgag
    10501 gcaacagtta tttcaacgac atttagtgta gtttctatca cctttgcaat tgtagttgct
    10561 gaaacagtgc acatgcaaaa tcaatttttt gctttctatt tatcagtgat agtatcctgc
    10621 ctcgttgcag cagtcattat gccaagaatt tggcctttaa ataaaattcc tgatgagtat
    10681 gctaaggagg tacctgaaag tgcgcgtact gaggcattgc cagaaggaaa gacggcattg
    10741 agacatggct ttgatactgc aacagaagtt ggtattaaag caccgggcgt tattgacttt
    10801 tttaagtcag gattaaaaac agtcattgat atgtggtttg ttattttgcc tgtagtgatg
    10861 agtattggta ctattgctac cattattgcg aactacacgc ccttttttgt catattgggt
    10921 aaaccttttg ttccattttt agagttaatg caaatacctg aagcggcaca ggcttctcaa
    10981 acgattatta tcggttttgc cgatatgttt ttaccttcta tattaattga aggtgtacaa
    11041 aatgatataa ctcgttttgt tattggggca cttagtatat cgcagttaat atacttgtct
    11101 gaagtaggtg gcgtcatact tggttcgaag attcctgtaa gcatcggcaa attgtttatg
    11161 attttcttga ttagaacaat cattacacta ccaattattt ctttgatggc acatcttttg
    11221 ttataaatat atgtgaaaaa ggacaactgt taaaattatg agaacagttg tccttttgta
    11281 tttgtt
//
LOCUS       DAOBBH010000076        11285 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.076, whole genome shotgun sequence.
ACCESSION   DAOBBH010000076 DAOBBH010000000
VERSION     DAOBBH010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11285
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.076"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(37..585)
                     /gene="hxlB"
                     /locus_tag="P6L01_001440"
     CDS             complement(37..585)
                     /gene="hxlB"
                     /locus_tag="P6L01_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HDP3030512.1"
                     /translation="MAKFSDYQLILDELKMTLSHVEADEFSTFASKILHAEHIFVAGK
                     GRSGFVANSFAMRLNQLGKQAHVVGESTTPAIKSNDVFVIISGSGSTEHLRLLADKAK
                     SVGADIVLITTNKDSAIGNLAGTNIVLPAGTKYDEQGSAQPLGSLFEQASQLFLDSVV
                     MGLMTEMNVTEQTMQQNHANLE"
     gene            complement(587..1219)
                     /gene="hxlA"
                     /locus_tag="P6L01_001441"
     CDS             complement(587..1219)
                     /gene="hxlA"
                     /locus_tag="P6L01_001441"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HDP3030513.1"
                     /translation="MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAV
                     KHMADNISNVKVLADMKIMDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKN
                     NKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLESLRTVKSVIKNS
                     KVAVAGGIKPDTIKDIVAESPDLVIVGGGIANADDPVEAAKQCRAAIEGK"
     gene            complement(1296..2054)
                     /gene="nagB"
                     /locus_tag="P6L01_001442"
     CDS             complement(1296..2054)
                     /gene="nagB"
                     /locus_tag="P6L01_001442"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDP3030514.1"
                     /translation="MKVLNLGSKKQASFYVACELYKEMAFNQHCKLGLATGGTMTDLY
                     EQLVKLLNKNQLNVDNVSTFNLDEYVGLTASHPQSYHYYMDDMLFKQYPYFNRKNIHI
                     PNGDADDMNAEASKYNDVLEQQGQRDIQILGIGENGHIGFNEPGTPFDSVTHIVDLTE
                     STIKANSRYFKNEDDVPKQAISMGLANILQAKRIILLAFGEKKRAAITHLLNQEISVD
                     VPATLLHKHPNVEIYLDDEACPKNVAKIHVDEMD"
     gene            2330..2692
                     /locus_tag="P6L01_001443"
     CDS             2330..2692
                     /locus_tag="P6L01_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YojF family protein"
                     /protein_id="HDP3030515.1"
                     /translation="MLEPIKEQEVIDLLASFEHKPVYLHVETTNGAYANHFDQRVFNA
                     GTFLRNIQVTYTHAQLKGGNKEPYRIGLKLSNGGWVYVQGLTHFEVNEHDEFLIAGFN
                     YEGQLAAALQISERPFNL"
     gene            2706..3371
                     /gene="bshB2"
                     /locus_tag="P6L01_001444"
     CDS             2706..3371
                     /gene="bshB2"
                     /locus_tag="P6L01_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB2"
                     /protein_id="HDP3030516.1"
                     /translation="MTDERHVLVIFPHPDDETFSSAGTLASYIQKGIPVTYACLTLGQ
                     MGRNLGNPPFATRESLPSIRERELEEACKVIGITDLRKMGLRDKTVEFEPYEHIDGMI
                     KSLIDDTNPSLIISFYPGYAVHPDHEATADAVIRTVERMPKEERPRLTLVAFSNDATE
                     ALGEPDIQNDITDFKELKIKAFEAHASQTGPFLKQLASPEIDGKQHSFLTVEPYWTYH
                     FKS"
     gene            3384..4262
                     /locus_tag="P6L01_001445"
     CDS             3384..4262
                     /locus_tag="P6L01_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HDP3030517.1"
                     /translation="MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFL
                     FEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIEL
                     EFNTLYQVLRTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDK
                     VTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQNIVDDYKNKILDAM
                     EANASSILYPILKRPDEKRVTERAYENPRFVEDLIRLIAADLVEFDWLDGFDIECRNE
                     ESIHQHDAFAKLKYRK"
     gene            4544..6034
                     /locus_tag="P6L01_001446"
     CDS             4544..6034
                     /locus_tag="P6L01_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDP3030518.1"
                     /translation="MNYFVGNSLGVNLTGIEKAIINRLNLFKEMGRPAQCVFLSWNRY
                     LYRNAQNYITSSDYINMYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRI
                     YDQERFLMYAHFQDPKYRILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLC
                     EFFYNPEGDTKLEKYFSYKDGKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGD
                     LFFSDRNVYTAPIFNLTPESIPVVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYR
                     AIIVSTEQQKLDVEKRINHTIPIVNIPVGYSETIDTPVQTLDQRSVKLISVARYSPEK
                     QLHQQIELIKRLVSYVPKIELHMYGFGSESKKLNELIQKYGLENHVYLRGFLSNLNQE
                     YSDAYLSLITSNMEGFSLALLESLAHGVPVISYDIKYGPNELITPDFNGYLITKNDED
                     ALFDKVKYVIDHPEVQQRLSKGSLAKAQQYSKASLIKQWDQFVRLI"
     gene            complement(6159..7631)
                     /locus_tag="P6L01_001447"
     CDS             complement(6159..7631)
                     /locus_tag="P6L01_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDP3030519.1"
                     /translation="MNYILGTILESKITGVEKAQINRLKLFKQHGISSKCVYVKWNPY
                     SYTYAKQHQIENDVFTMYDYFQKAINYKKTKQVNWIQYWEKSCRYTLKFVENSNDVRI
                     YDEEQFVMYAHFLDKQYHQLNYVNYFDHKRRKVKRELYDGRGFLSCSRILGEGQRIVL
                     ENYYTPNGEIVIQKYFDDIKGKNTLTKVILNEDQQQQFFDTEDELVQYFLHQLCKNND
                     QIILDRPHELGNVIAGLNQSIPVVVVLHSTHLSGTGNGIKSFYKTVFNNLTRYKAIVV
                     STEQQCQDISQYIENKIPVINIPVGYVANLKYQFDINQKEKNHIISIARLVENKQIKH
                     QIEVIKQLVTKHPNIQLNIYGHGNGLSEYRQLVEDYHLSEHVKFHGFKTHINEEIAKA
                     ELMLSTSKMEGFGLAILESLSVGTPVISYDIDYGPSELIQDGFNGYLVPKGDINQMVE
                     KVDQLLNNTQKLQQFSINSIESAQQYNATTISTKWQNILN"
     gene            complement(7751..11248)
                     /gene="sdrE"
                     /locus_tag="P6L01_001448"
     CDS             complement(7751..11248)
                     /gene="sdrE"
                     /locus_tag="P6L01_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrE"
                     /protein_id="HDP3030520.1"
                     /translation="MINRDNKKAITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAENTSTENAKQDDATTSDNKEVVSETENNSTTENDSTNPIKKETNTDSQP
                     EAKEESTTSSTQQQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNY
                     TNNDVTTKPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPVNVSKE
                     ELKNNPEKLKELVRNDNNTDRSTKPVATAPTSVAPKRLNAKMRFAVAQPAAVASNNVN
                     DLITVTKQTIKVGDGKDNVAAAHDGKDIEYDTEFTIDNKVKKGDTMTINYDKNVIPSD
                     LTDKNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKARLTLYSYIDKQA
                     VPNETSLNLTFATAGKETSQNVSVDYQDPMVHGDSNIQSIFTKLDENKQTIEQQIYVN
                     PLKKTATNTKVDIAGSQVDDYGNIKLGNGSTIIDQNTEIKVYKVNPNQQLPQSNRIYD
                     FSQYEDVTSQFDNKKSFSNNVATLDFGDINSAYIIKVVSKYTPTSDGELDIAQGTSMR
                     TTDKYGYYNYAGYSNFIVTSNDTGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQGTDSK
                     EKPMANVLVTLTYPDGTTKSVRTDANGHYEFGGLKDGETYTVKFETPAGYLPTKVNGT
                     TDGEKDSNGSSVTVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDANEPGIK
                     DVKVTLKDSTGKVIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTVKNTTAEDKD
                     SNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKDVKVTL
                     LNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTVTNTTEDDKDADGGEV
                     DVTITDHDDFTLDNGYFEEDTSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDAGKHTPVKPMSTTKDHHNKAKALPETGSENNGSNNATLFGG
                     LFAALGSLLLFGRRKKQNK"
BASE COUNT         3433 a         1948 c         1783 g         4121 t
ORIGIN      
        1 ttctgcctag gcatcatatt atcgactatc tttattttat tctaaattag catgattttg
       61 ttgcatcgtt tgttccgtaa cattcatttc agtcatcaat cccattacaa cactatctaa
      121 aaataattga gatgcttgtt caaacaaact tcctaatggt tgtgccgagc cttgttcatc
      181 atattttgta cctgcaggca aaacgatgtt cgtcccagct agattgccta ttgcagaatc
      241 tttatttgta gtaattaata cgatgtcagc acctactgat tttgctttgt ctgctaataa
      301 tcttaaatgt tccgtggaac ctgaaccaga gataattaca aatacatcat tcgacttaat
      361 cgcaggtgtc gttgattctc caacaacatg tgcctgtttg ccgagctgat ttaagcgcat
      421 tgcaaaacta ttcgccacga atcctgaacg tcctttgcca gctacaaata tatgttcagc
      481 atgtagtatt ttggatgcaa aagttgaaaa ctcatccgct tcaacatgtg acaaagtcat
      541 ctttaattca tctagaatta attgatagtc actaaattta gccatattac ttaccttcga
      601 ttgcagcgcg acattgtttt gcagcttcta ctggatcatc tgcatttgcg attccgccac
      661 caacaataac aagatcagga ctttcagcga caatatcttt aattgtatct ggtttaattc
      721 cacctgctac tgcaacttta gaatttttaa taacagattt aacggttctt aaactttcta
      781 atggtgattg cccttctgct tgtaaatcat aaccagtgtg tactgcaata taatcagcac
      841 ccatttcatc tagttctttt gcacgttttt ctaaatcttg aacagcaatc atatcaacta
      901 gtaattgttt attattttta tgagcttctt caatagctgc tttaattgat gcatcttctg
      961 caacacctag tattgtaatt acatccgcgc caaatttaat tgcttggcta acttcataat
     1021 cagctgcatc cataattttc atgtctgcta atacttttac attactaatg ttgtctgcca
     1081 tatgtttaac tgctggtaaa ccttcgttgt aaatgattgg cgtaccgatt tctacgatat
     1141 ctacataatc ttttacttta tttgctaact cagccgcgtc ttctttgttt aataaatcaa
     1201 ttgctaattg taattccact aaaaatcatc cttccaatgc tttttatctt tctattggaa
     1261 cctttcccga ggcatttctt aattaaacat tgcaatcaat ccatttcatc gacatgaatt
     1321 ttcgcaacat ttttcgggca agcttcgtcg tctaaatata tctcaacatt cgggtgtttg
     1381 tgaagtaatg tggctggaac atcaacagaa atttcctgat ttaataaatg tgtaatagca
     1441 gcacgtttct tttcaccaaa tgcgagtaaa atgatacgtt tggcttgaag aatattagca
     1501 agtcccatcg aaatggcttg ctttggaaca tcatcttcgt ttttaaaata tcgactatta
     1561 gccttaatag tactttcagt caaatcaacg atatgagtaa cgctatcaaa cggcgtacca
     1621 ggttcattaa atccaatatg accattttca ccaataccta aaatttgaat atcacgttga
     1681 ccttgttgtt ctaaaacgtc attatatttt gacgcttccg cattcatatc atcggcatct
     1741 ccatttggaa tatgaatgtt ctttctatta aaataaggat attgtttgaa aagcatgtca
     1801 tccatataat agtgataact ttgcggatgt gatgcggtta aacctacata ttcgtctaaa
     1861 ttaaacgtgg atacattgtc tacgtttaac tgatttttat ttaataactt aacaagttgt
     1921 tcatacaaat ctgtcattgt accaccagtt gctaaaccta gtttacagtg ctgattaaat
     1981 gccatctctt tatataactc acatgcaaca tagaatgatg cttgtttttt cgatcctaag
     2041 tttaatactt tcattgttta catgcctcca caaatttaat acattttaaa aacttatgaa
     2101 ttcattcata caccattaat atagcatcat ttttaatgtt taacactttt caaattccaa
     2161 caaatttttg atttttaaaa gtaaaaaact aatgctgtat gattacgtaa tcctgagtaa
     2221 ttacccttca tgaaaattaa tttataaata attgagcaat cgtatacaat tatcgaatag
     2281 tttttagtaa aatagaaaga taaacacatt gatagtgtaa ggagtgaacg tgttggaacc
     2341 aattaaagaa caagaagtta tagatttact tgcttctttt gaacataaac ctgtgtatct
     2401 gcatgttgaa acgacaaatg gggcctatgc gaaccatttc gatcaacgtg tttttaatgc
     2461 tggtacattt ttaagaaaca tacaagtaac atatacgcat gcacaactaa aaggtggtaa
     2521 taaagaacct tatcgcattg gtcttaaact atcaaatggt ggttgggttt atgtacaagg
     2581 tctaacgcat tttgaggtca atgaacatga tgaattttta attgcaggtt ttaattacga
     2641 aggtcaactc gctgcagcac ttcaaattag tgagcgacca tttaatttat agaggaggca
     2701 tcaacatgac tgacgaaaga catgtattag taatatttcc acatcctgat gatgaaacct
     2761 tctcatctgc aggcacatta gcaagttata tccaaaaagg catacctgta acatatgcat
     2821 gtttaaccct aggacaaatg ggcagaaatt taggtaatcc cccatttgca acacgcgaat
     2881 cattaccaag tattcgtgag cgtgaactag aagaagcttg taaagttatt ggtattactg
     2941 atttaagaaa aatgggttta cgtgacaaaa ctgtcgaatt tgaaccctac gaacatatag
     3001 atggtatgat taaatcttta atcgatgata caaatccatc attgattatt tcattttatc
     3061 ctggatatgc agtacatcct gatcacgaag caacagcaga cgctgtaatt cgaacggttg
     3121 aacgcatgcc taaagaagaa cgccctcgtt taactttagt tgcgttcagt aatgatgcaa
     3181 ccgaagctct tggagaacct gatattcaaa atgatattac agattttaaa gaacttaaaa
     3241 ttaaggcatt tgaagcacat gcttctcaaa caggtccatt tttaaaacaa ttagcaagtc
     3301 ctgaaatcga tggtaaacaa cacagctttt taacagtcga accatattgg acatatcact
     3361 ttaaatctta acggaggaaa aaaatgactg aatttgattt atcgacacga gaaggtcgtt
     3421 ggaaacactt tggttccgta gatcctattg aaggcacgaa acctactact aagaacgaaa
     3481 tgacagattt acaaagtaca cataaagatt ttttatttga aatagaagaa gttggtatta
     3541 aaaacttagt gtaccctgta cttgttgacc agtatcaaac agctggcaca tttagttttt
     3601 caacaagttt aacaaaagac gaaaaaggta ttaatatgag ccgtataatt gaaagtgttg
     3661 aaaagcacta tgataatggt attgaacttg aatttaatac actttatcag gtgttacgca
     3721 ctttacaaac gaatatgaaa caaaatgcgg caggcgttga tgtttctggg aaatggttct
     3781 ttgaccgcta tagtccaacc acaaatatta aagcagttgg taatgccgat gtcacttatg
     3841 gattagcaat tgacggtgat aaagttacac gtaaagaact tactattgaa gcaactgtca
     3901 caacgctatg cccttgttca aaagaaataa gtgagtattc agcgcacaac caacgtggtg
     3961 ttgtcactgt taaaacatat attaataaag atcaaaacat agttgatgac tataaaaaca
     4021 aaattttaga tgcaatggaa gctaatgcaa gttcaattct ataccctatt ttaaaacgtc
     4081 ctgatgaaaa acgtgtgaca gagcgtgcat atgaaaatcc acgttttgtc gaagacttaa
     4141 ttcgtttaat tgccgcggac ttagttgaat ttgattggtt agacggtttc gatatcgaat
     4201 gtcgtaatga agagtctatc catcaacatg atgctttcgc taaattaaag tatcgcaaat
     4261 aatatcatat gattaattca aacgcgtaac attagttgcg cgtttttatt ataccttcta
     4321 gagttgtata tctcttttat aaatcttctc atttgatgtg cctgaggcat aaatcaatat
     4381 cctaagctct acaatgatat attggtcgta gttgattgaa taactactat cattataaat
     4441 atcaaaattt gttgttttat aaataaacac atcatccagt taaaagaagt aattaaatat
     4501 tatgtgatat aattataaaa aatagatata agtggtgttg taaatgaatt attttgtagg
     4561 taatagttta ggtgttaatt taactgggat agaaaaagca attattaatc gtttaaattt
     4621 atttaaagaa atgggaagac cagcgcaatg tgtatttctg tcatggaata gatatttata
     4681 tagaaatgcc caaaattata ttactagttc tgactatatt aatatgtatg atttcttcca
     4741 agaagcaaca tatttagaac gcaatgaacc atttgactgg ttgtcgtatt ggactgatga
     4801 atgtcattat actttaaaac atgttgaaaa tagtcatgac tttagaatat atgatcaaga
     4861 gcgtttctta atgtatgccc attttcaaga tcctaaatat cgtatattag attatgtgaa
     4921 tcattttgat agtcaaagac gaaaagtaaa acgtgatttt tatgatgtgc gtggcttttt
     4981 aagttgtagt aggattcttg ttgataaaca acaaacatta tgtgaatttt tctataatcc
     5041 agaaggtgac acaaaattag aaaagtattt ttcttacaag gatggcaaac cagaagtaca
     5101 aaaaattatt gtttactatg ctaacaaaca atatttcttt aataatgaaa cggaattagg
     5161 cgcatttttc attaaacaac tatatcaaca tggggattta tttttcagtg accgtaatgt
     5221 gtatacagca cctattttca acttaacacc tgagtcaatc ccagtagtag ctgtgcttca
     5281 cagtacacac attaaaaata ttgatgcatt agattcatcg ccatttaaaa atgtatataa
     5341 agcgatgttt gaaaatttat ctcgatatcg tgcaattatc gtttctactg agcagcaaaa
     5401 attagatgtt gaaaaacgta ttaaccatac tattccaatt gttaatattc ctgtcggcta
     5461 tagtgaaaca attgatacac cagtccaaac attagatcag cgcagtgtta aattaatttc
     5521 cgttgctcgt tattcacctg aaaaacaatt acatcaacaa attgaattaa ttaaacgctt
     5581 agtatcgtat gtcccaaaaa tagagcttca tatgtatggt tttggttctg aaagtaaaaa
     5641 attaaatgaa ttgatacaaa aatatggatt ggaaaatcat gtttacttac gtggcttttt
     5701 aagcaatcta aaccaagaat acagcgatgc ttacttaagt ttaataacaa gtaacatgga
     5761 aggtttctct ttagcgctac ttgaatcttt agcacatggt gttccagtta ttagttatga
     5821 tattaaatat ggacctaacg aattgattac gcctgatttc aatggttatt taattactaa
     5881 aaatgatgaa gatgccttat ttgataaagt aaaatatgtg attgatcatc ctgaagtaca
     5941 acaacgactg tcaaaaggta gtttggcaaa agcgcaacaa tatagtaaag cgtcgttaat
     6001 taaacaatgg gaccaatttg ttcgtcttat ataaatgtac ttcaccttta tttttataaa
     6061 aaaaagcgtt ttcctatgtc atttaactaa acatgtaagt tcgtatgaac gttgtttgtt
     6121 aaatagatga ttctaggaaa atgctttttt cttttgactt agtttaaaat attttgccac
     6181 tttgtactga tagtagttgc attgtactgt tgtgcagatt ctatgctatt aatcgaaaac
     6241 tgttgcaatt tttgagtatt atttagtaat tgatcgacct tttcaaccat ttgattgatg
     6301 tcacctttag gtactaaata gccattaaat ccatcttgaa tcagttctga tggcccataa
     6361 tctatatcat aactgatcac tggtgtacct actgaaagcg actctaaaat tgctaagcca
     6421 aaaccttcca ttttacttgt cgataacatc agttctgctt tagcaatctc ttcattaata
     6481 tgcgtcttaa aaccatgaaa tttaacatgt tccgataaat gataatcttc tacaagttgt
     6541 cgatattctg acaaaccatt tccatgtcca taaatattca attgaatatt gggatgtttt
     6601 gttactaatt gcttgatcac ttcaatttga tgtttaattt gtttattttc aacgaggcga
     6661 gcaattgata tgatatgatt tttctccttt tgattgatgt caaattgata ctttaaattt
     6721 gccacgtagc caaccggaat attgataact ggtattttat tttcaatata ttgtgaaata
     6781 tcttggcatt gctgttctgt tgatacaaca atcgctttat aacgtgttaa attattaaat
     6841 actgttttat aaaaactttt tataccatta ccggtaccgg ataaatgtgt actatggagc
     6901 acaacaacaa ctggaatact ttgatttaat cccgctataa catttcctaa ttcatgagga
     6961 cgatctaata tgatttgatc attattttta cataattgat ggagaaaata ttgaactaat
     7021 tcatcttctg tatcaaaaaa ttgttgctgc tggtcttcat ttaagataac ctttgtgagc
     7081 gtgttttttc cttttatatc gtcgaaatat ttttggatga cgatttcccc attaggtgta
     7141 tagtaatttt cgagtacaat ccgttgtcct tcacctaaaa ttcgagaaca acttaaaaag
     7201 cctcttccat catacaattc gcgttttact tttcttcttt tatgatcaaa ataattcaca
     7261 tagtttaatt gatgatactg tttatctaaa aaatgagcat acattacaaa ttgctcttca
     7321 tcatatattc tgacatcatt tgaattttcc acaaatttca atgtgtacct acatgacttt
     7381 tcccaatact gtatccagtt aacttgcttt gtctttttat aattgattgc tttttgaaaa
     7441 tagtcataca ttgtaaatac atcattttca atctgatgtt gcttcgcata tgtgtatgaa
     7501 taaggattcc atttaacata tacacatttt gaagatatgc cgtgttgttt gaacaacttc
     7561 aatctattta tttgcgcttt ttctacacct gtaattttac tttctaaaat tgttcctaaa
     7621 atgtaattca tattatcgcc tcatataagt tttattccgt atctttattg tttattttat
     7681 atgaaaaata catctattgc atgtgtaatc ataaaaaaaa ccaggccaca aggacctggg
     7741 tcatattgta ttatttgttt tgttttttgc gacgaccgaa taacaataat gaacctaatg
     7801 ctgcaaataa tccaccaaat aacgttgcgt tatttgagcc gttattttca ctacctgttt
     7861 ctggtaatgc ttttgcttta ttgtgatggt ctttagtagt actcattggt ttaacaggtg
     7921 tatgttttcc tgcatccgag tccgaatcgc tatctgagtc tgaatcactg tctgagtctg
     7981 agtcgctatc tgagtctgag tcgctgtccg aatctgagtc gctatctgag tctgagtcgc
     8041 tgtctgaatc tgagtcgcta tctgagtctg aatcactgtc cgaatctgag tcgctatctg
     8101 aatctgaatc gctgtctgag tctgagtcac tgtctgagtc tgagtcgcta tctgagtctg
     8161 agtcactgtc tgaatctgag tcgctatctg agtctgaatc gctgtctgaa tctgagtcgc
     8221 tatctgagtc tgaatcgctg tccgaatctg agtcgctatc tgagtctgaa tcgctgtccg
     8281 aatctgagtc gctatctgag tctgagtcgc tgtctgagtc tgagtcgcta tctgagtctg
     8341 agtcgctgtc tgaatctgaa tcgctatctg agtctgaatc actgtctgaa tctgagtcgc
     8401 tgtctgagtc tgaatcactg tctgagtctg aatcactgtc tgatgtatct tcttcgaagt
     8461 atccgttatc aagtgtgaaa tcatcatgat ccgtaattgt tacgtcaact tcgccaccat
     8521 cggcatcttt atcatcttca gttgtatttg taactgtttg tgttaagcca gcaggctttt
     8581 caaaaataac tttgtattta ccgctatcta aattatcgaa acgatattta ccattttcat
     8641 ctgttttagt tgttccaatt acttcgcctt tttcatttaa taaagtaact ttaacatctt
     8701 tgataccttt ttcagttgaa tcttgtttac cgtctttatt actgtcgtac caaacataat
     8761 cacctaaact gtattttggt gttttataga aaccactgtc taatgtcata ttatctgcat
     8821 ctttaatgac acctgttgtt gttaaaccat tagaatcttt atcttcagct gtagtatttt
     8881 taaccgttgg cgtgtaacct gctggtgttt caaattctac tgtatagtta ccattatcta
     8941 aatctgtaaa tttatattta cccgaggcat cagtagtagt tgtaccaata acttttccag
     9001 tactatcttt taatgtaacc ttaacatctt tgataccagg ttcattagca tcttggatac
     9061 catctttatt tgtatcttcc catacatagt caccaagatt atatttaggt tctttataaa
     9121 aaccagtgtc taaagacata tcatctttac cattaatttt aacagttaca gaactaccat
     9181 ttgagtcttt ttcaccatca gttgttccat ttacttttgt tggaagatat ccagctggcg
     9241 tttcgaattt aactgtataa gtttctccgt ctttcaaacc accgaattca taatgaccgt
     9301 tagcatctgt tcttactgat tttgtagtac cgtccgggta agttaatgta actaaaacgt
     9361 ttgccattgg cttttctttc gaatctgtac cttggacacc gtctttatca acgtcttccc
     9421 atacatagtc accaattttg tataactttt cttcaggttt aacagtaccg tcgccaccgc
     9481 cagtgtcatt agaagttacg atgaagtttg aatatcctgc ataattataa taaccatatt
     9541 tatcagttgt tctcatacta gtaccttgag caatatctag ttcgccatct gatgtaggtg
     9601 tatatttact aacaactttg ataatatagg ctgaattaat atcaccaaaa tccaatgttg
     9661 ctacattatt actaaatgat tttttattat caaattgact tgttacatct tcgtattgac
     9721 taaaatcata gattctatta ctttgaggca attgttgatt agggttaact ttataaactt
     9781 ttatttctgt attttggtca ataatggtac taccatttcc tagtttaata tttccataat
     9841 catctacttg actaccagct atatcaactt tagtgttagt tgctgttttt ttcaaaggat
     9901 taacataaat ttgttgttca atagtttgtt tgttttcatc taactttgta aagatagatt
     9961 gaatgtttga atcaccatga accattgggt cttgataatc aacagaaacg ttttggctag
    10021 tttctttacc tgctgttgca aacgttaaat tcaaactagt ttcattaggt actgcttgct
    10081 tatcaatata tgagtataaa gttaaacgtg cttttatatc ttcatattta tctacataat
    10141 ctgtaaatgt atatgtgatt tgcttagtcg ctttatcaaa tgttcctttg gcaatgacct
    10201 ctcctgatgg atcagtaata tcgataggat catttttatc tgttaaatcc gaaggaatta
    10261 cattcttatc ataattaatc gtcattgtat cgcctttttt gactttattg tcaattgtaa
    10321 actctgtatc atattcaata tctttaccgt catgcgctgc tgccacatta tctttaccat
    10381 cgccaacttt gatcgtctgt ttcgtaactg taattaagtc atttacatta tttgaagcaa
    10441 ctgctgctgg ttgtgcaact gcaaaacgca ttttcgcatt taatcgtttt ggtgcaacac
    10501 ttgttggagc tgtagcaact ggtttagttg aacgatctgt attgttatca tttctaacta
    10561 attcttttaa tttctcagga ttatttttaa gttcttcttt tgacacattt actggttctt
    10621 ttggattagt tgcatctgta acttgattgt ctacttttga aggcgttggt tgcggttgtg
    10681 aattttcaat atttgtagat tcttgaggtg tagttggttt tgtttgaatt tcacttgtag
    10741 atggttttgt agttacatcg ttatttgtat aatttggtgc tttcttctct tctaaaataa
    10801 cagatgtatc ttctgtcgca gttttatcag ttgaaggttt aacattttct ttttcaatgt
    10861 tttgaggctt agtttcagtt gtagctgtaa cgttattttg ctgttgttga gtacttgatg
    10921 tagttgattc ttctttagct tctggttgtg aatcagtatt tgtttctttc ttaattggat
    10981 ttgttgaatc attttctgtt gtcgaattat tttcagtttc cgacactact tctttattat
    11041 cactagtcgt tgcatcatct tgtttcgcat tttctgtact agtgttttca gcagctttag
    11101 cttcttggtt ccctagacca aaaatcaatg tcgtacctac taaaatcgat gcagttccta
    11161 cagtatactt tctaatcgaa aatttgttta agcgatttga aatcataccc ttttttgtta
    11221 ttgccttttt attatccctg ttaatcatat aaatctcctt taaaatatca atatttgcaa
    11281 aaaat
//
LOCUS       DAOBBH010000077        11244 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.077, whole genome shotgun sequence.
ACCESSION   DAOBBH010000077 DAOBBH010000000
VERSION     DAOBBH010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11244
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.077"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            33..1403
                     /locus_tag="P6L01_001449"
     CDS             33..1403
                     /locus_tag="P6L01_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HDP3030521.1"
                     /translation="MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMI
                     LGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLILMTRGNVV
                     IVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMV
                     PWGGPMARVAAVLKAKSVNELWYGLIPIQIIGFILVMLFAVYLGFKEQKRIKKAIERN
                     ELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTKSWIKWVNTALTLAVILSMLINIAP
                     PEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLK
                     AIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSV
                     STAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAML
                     MGIVTI"
     gene            1593..3443
                     /gene="nrdD"
                     /locus_tag="P6L01_001450"
     CDS             1593..3443
                     /gene="nrdD"
                     /locus_tag="P6L01_001450"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDP3030522.1"
                     /translation="MNQIEEALTGLISKDPAIVNENANKDSDTFSTMRDLTAGIVSKS
                     YALNYLLPKHVADAHQRGDIHFHDLDYHPFQPLTNCCLIDAKNMLHNGFEIGNANVTS
                     PKSIQTASAQLVQIIANVSSSQYGGCTVDRVDELLSTYARHNEEQHRNIAKQFVKESE
                     IDRYVDQQVTKDINDAIESLEYEINTLYTSNGQTPFVTLGFGLGTDHLSRKIQQAILN
                     TRIKGLGKDRTTAIFPKLVFSIKKGTNFSPQDPNYDIKQLALKCSTKRMYPDILNYDK
                     LVEILGDFKAPMGCRSFLPSWKDAEGHFENNGRCNLGVVTLNLPRMALESAGNMTKFW
                     EIFYERIDVLHDALLYRINRLKDAVPNNAPILYKSGAFNYKLKETDDVAELFKNKRAT
                     ISMGYIGLYETATVFYGPDWETSQEAKAFTLEILKEMKRYQTKWTELYDIWFSIYSTP
                     SESLTDRFCRLDQERFGDIKDITDKGYYQNSFHYDVRKDVTPFEKLDFEKDYPYYASG
                     GFIHYCEYPKLQHNLKALEAVWDYSYDKVGYLGTNIPIDHCYECDYDGDFEATEKGFK
                     CPNCGNDNPKTVDVVKRTCGYLGNPVQRPVIKGRHKEICARVKHMKAPKE"
     gene            3440..3976
                     /gene="nrdG"
                     /locus_tag="P6L01_001451"
     CDS             3440..3976
                     /gene="nrdG"
                     /locus_tag="P6L01_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDP3030523.1"
                     /translation="MTLLDIKQGQGYIAKIESNSFVDGEGVRCSVYVSGCPFNCVGCY
                     NKASQKFRYGEKYTDEILAEILDDCDHDYISGLSLLGGEPFCNLDITLNLVKAFRARF
                     GNTKTIWVWTGFLYEYLANDCTERRELLSYIDVLVDGLFIQHLFKPDLPYKGSLNQRI
                     IDVQQSLSHARMIEYIVS"
     gene            4076..4186
                     /locus_tag="P6L01_001452"
     CDS             4076..4186
                     /locus_tag="P6L01_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030524.1"
                     /translation="MIEYDLTIILIPILKVAYPFFKKVERKKWYFSGKEV"
     gene            complement(4202..4363)
                     /locus_tag="P6L01_001453"
     CDS             complement(4202..4363)
                     /locus_tag="P6L01_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030525.1"
                     /translation="MAIIEYLYNFSRNKQNFNSAKTAVLSYDLTNLTNFTRTAFKNIY
                     TFLTNIYLL"
     gene            4495..6117
                     /locus_tag="P6L01_001454"
     CDS             4495..6117
                     /locus_tag="P6L01_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDP3030526.1"
                     /translation="MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAI
                     SDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSD
                     ILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLV
                     FVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIE
                     RLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIG
                     PTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAP
                     FIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNT
                     HGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRA
                     NRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDR
                     IKLETRAEKLKEVERRSLRIVQVSEEEQDDNL"
     gene            complement(6288..6851)
                     /locus_tag="P6L01_001455"
     CDS             complement(6288..6851)
                     /locus_tag="P6L01_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HDP3030527.1"
                     /translation="MTRSQNKQQQLEQAKDIVINSIGQTMDLYGTNRSVGNLYGTMVF
                     EGSMTLDEMRHQLQMSKPSMSAGVKKLQEFDIVKQQFTRGSRKQHFIAEKDFFIFFRN
                     FFTKKFQREIDINVEAVKDAQAIINPLLESSDLTEAETQEAQKIKAQLDHTHVYYEWL
                     EQLTEAIESGEIFKYFPIPEQPSDSEN"
     gene            complement(6940..7035)
                     /locus_tag="P6L01_001456"
     CDS             complement(6940..7035)
                     /locus_tag="P6L01_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030528.1"
                     /translation="MKNVSIYKTTQELSFVSSLDFYLTKHLTLML"
     gene            7104..8594
                     /gene="betB"
                     /locus_tag="P6L01_001457"
     CDS             7104..8594
                     /gene="betB"
                     /locus_tag="P6L01_001457"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HDP3030529.1"
                     /translation="MELLKHLSQRQYIDGEWVESTNKNTRDIINPYNQEVIFTVSEGT
                     KEDAERAILAARRAFESGEWSQETAEKRGKKVRAIADKIKEHREALARLETLDTGKTL
                     EESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPL
                     LQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGD
                     VMSGHKEVDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQ
                     ALNGGYFHAGQVCSAGSRILVQNSIKDKFEQALIDRVKKIKLGNGFDADTEMGPVIST
                     EHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVF
                     GPVVTVEGFETEQEAIQLANDSIYGLAGAVFSKDIGKAQRVANKLKLGTVWINDFHPY
                     FAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK"
     gene            8855..10564
                     /gene="betA"
                     /locus_tag="P6L01_001458"
     CDS             8855..10564
                     /gene="betA"
                     /locus_tag="P6L01_001458"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HDP3030530.1"
                     /translation="MSNKNKSYDYVIIGGGSAGSVLGNRLSEDKDKEVLVLEAGRSDY
                     FWDLFIQMPAALMFPSGNKFYDWIYSTDEEPHMGGRKVAHARGKVLGGSSSINGMIYQ
                     RGNPMDYEGWAEPEGMETWDFAHCLPYFKKLEKTYGAAPYDKFRGHDGPIKLKRGPAT
                     NPLFQSFFDAGVEAGYHKTPDVNGFRQEGFGPFDSQVHRGRRMSASRAYLHPAMKRKN
                     LTVETRAFVTEIHYEGRRATGVTYKKNGKLHTIDANEVILSGGAFNTPQLLQLSGIGD
                     SEFLKSKGIEPRVHLPGVGENFEDHLEVYIQHKCKEPVSLQPSLDIKRMPFIGLQWIF
                     TRTGAAASNHFEGGGFVRSNNEVDYPNLMFHFLPIAVRYDGQKAAVAHGYQVHVGPMY
                     SNSRGSLKIKSKDPFEKPSIRFNYLSTEEDKKEWVEAIRVARNILSQKAMDPFNGGEI
                     SPGPEVQTDEEILDWVRRDGETALHPSCSAKMGPASDPMAVVDPLTMKVHGMENLRVV
                     DASAMPRTTNGNIHAPVLMLAEKAADIIRGRKPLEPQYIDYYKHGVHDENEGAIEVKP
                     YAK"
     gene            10842..11054
                     /locus_tag="P6L01_001459"
     CDS             10842..11054
                     /locus_tag="P6L01_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HDP3030531.1"
                     /translation="MSFYEYIQTFKDDKTPLGELAIWIKEDDSFPKQEKLTENILSYF
                     HQMSNIDHEFLEIVKRSLSLYDQLKS"
BASE COUNT         3804 a         1669 c         2205 g         3566 t
ORIGIN      
        1 aaattgtatt aaaagtggag ggagaaaata atatgaatag tgataatatg tggttaacag
       61 taatggggct cattattatt atttcaattg taggtttact cattgccaaa aagataaatc
      121 cagttgtagg tatgacaatc ataccttgct taggggcaat gattttagga tatagtgtga
      181 cagatttggt tggatttttt gctaaagggt tagatcaagt catcaacgtt gttattatgt
      241 ttatctttgc cattattttc tttggcatca tgaacgatag tggtttattc aagccgcttg
      301 tcaaacgctt aatattaatg acacgaggca atgtcgtcat tgtctgtgca atgacagctt
      361 taattggcac aatagcccaa ttagatgggg ccggtgcggt aacatttttg ctttctattc
      421 ctgcattatt acctttatat aaagcgttaa atatgaataa atatttattg attttactat
      481 tagcattaag cgcggcgatt atgaacatgg taccttgggg aggtccaatg gctcgtgtag
      541 ctgcagtgtt aaaagccaaa agtgtcaatg aattatggta tggattaata cctattcaaa
      601 taataggttt cattcttgtt atgttgtttg cggtatatct tggatttaaa gaacagaaac
      661 gtatcaaaaa agcaatagag agaaatgaat taccgcaaac acaagatata gatgtacata
      721 aattagttga agtatatgaa cgagatcaag atgtaaggtt tcctgtaaaa ggacgtgcaa
      781 gaacaaaatc atggataaaa tgggtgaata cagctttaac tttagctgtt attctatcga
      841 tgttaataaa tattgcgcca cctgaatttg cattcatgat aggtgtctca ttggcgcttg
      901 ttattaattt taaatcagtg gatgaacaaa tggagcgatt aagagcgcat gcgccgaatg
      961 cattaatgat ggctgcagtg attattgcag caggtatgtt tttaggtgta ctaaatgaaa
     1021 ccggtatgct taaagcgatt gcgaccaatt taatcaaagt gattcctgca gaagtaggac
     1081 catacttgca tattattgta ggtttacttg gcgtaccatt agatttacta actagcacag
     1141 acgcttatta ttttgctgtg ttaccgattg tcgaacaaac agcagggcaa tttggtgtac
     1201 cgtctgtatc aacagcttat tcaatggtca tagggaatat tataggtaca tttgtcagcc
     1261 cattttcacc agccttatgg ttggcaattg gtttagcaga ggcaaacatg ggcacgtata
     1321 ttaagtatgc attcttttgg atttggggat tcgccatcgt tatgttagta attgcaatgt
     1381 tgatgggcat tgtgacgatt taagttgcat aaacatgtcg aaatgacaga acaattttaa
     1441 aaattaatga aataattgtg tagaaatatg aatttcacta aatgttaata actttgtgac
     1501 gttttagtta acagactaat aaaaatttga aaatactata tatagtggta taacgtaatg
     1561 agtagacaca atatatagga agaaggggta aaatgaatca aatcgaagaa gcattaacgg
     1621 gtttgatttc taaagatcct gctattgtta acgaaaatgc caacaaagat agtgatacat
     1681 tttcaacaat gagagattta acagcaggta tcgtttctaa atcttacgca ttaaattatt
     1741 tattaccaaa gcacgttgca gatgcacatc aaagagggga catacatttt cacgacttag
     1801 attatcatcc attccaaccg ttaacaaact gttgtttaat agatgctaaa aatatgctac
     1861 ataatggatt tgaaataggc aacgcgaatg taacttcacc aaaatcaata caaactgcat
     1921 cagcgcagct tgtacaaatt atagccaatg tttccagcag tcaatatggt ggctgtacgg
     1981 ttgaccgcgt tgacgaatta cttagtacat atgcacgaca taatgaagaa caacatagaa
     2041 atatcgcaaa gcaatttgtc aaagaatctg aaattgatcg ttatgttgat caacaagtca
     2101 ctaaagacat caatgatgcg attgaaagtc tagaatatga aattaatacc ttatatacat
     2161 ctaatggaca gacacctttt gtaacattag gattcggctt aggtacagat catttaagtc
     2221 gcaaaattca acaagctatc ttaaatactc gtatcaaagg cttaggaaaa gaccgcacga
     2281 cagcgatttt cccaaaactt gtattttcaa ttaaaaaagg aaccaacttt agtccgcaag
     2341 atccgaacta tgacattaaa caactagcat taaagtgttc aacgaaacgt atgtatccag
     2401 atattttaaa ttatgacaaa ctcgtagaaa tattaggtga tttcaaagcg ccaatgggtt
     2461 gtcgttcatt tttaccaagt tggaaagatg cggaaggtca ttttgaaaat aatggtcgtt
     2521 gtaatcttgg tgttgttaca cttaatttac ctagaatggc attagaatct gccggtaata
     2581 tgacgaaatt ctgggaaatc ttttatgaac gtatcgatgt gttacatgat gcattacttt
     2641 atcgtataaa tcgtttgaaa gatgctgtac cgaataacgc accaatttta tataaaagtg
     2701 gcgcttttaa ctataaatta aaagaaacag atgatgttgc tgagttattt aaaaataaac
     2761 gtgcaacgat ttcaatgggc tatatagggt tgtatgaaac agctactgtt ttctatggtc
     2821 cagactggga aacatctcaa gaagcaaaag catttacgct tgaaattctt aaagaaatga
     2881 aacgttatca aacgaaatgg acagaattat atgacatttg gttcagtatt tacagtacgc
     2941 cgagtgaatc gctaacggat cgtttttgtc gtttagacca agagagattt ggagatatta
     3001 aagacattac agataaagga tattatcaaa actctttcca ttatgatgta cgtaaagatg
     3061 ttacaccttt tgaaaagtta gattttgaaa aagattatcc ttattatgcg agtggtggtt
     3121 tcattcacta ttgtgagtat ccgaaattgc aacacaattt gaaagcacta gaagcggtat
     3181 gggactactc ttatgacaaa gttggttact taggtacaaa tattccgatt gatcattgtt
     3241 atgaatgtga ttacgatgga gattttgaag caactgaaaa aggatttaaa tgcccgaact
     3301 gtggcaatga taatcctaaa acagttgatg tcgttaaacg aacatgtggt tacttaggca
     3361 atccagttca acgtccagta attaaaggcc gtcataaaga aatttgcgca cgagtaaaac
     3421 atatgaaagc gcctaaagaa tgacactttt agacattaaa caaggacaag gttatattgc
     3481 taaaatagaa tcaaatagct ttgttgacgg tgaaggagta agatgcagtg tttatgtatc
     3541 aggatgtcca tttaattgtg ttggatgtta taacaaagcc tcacaaaagt tcagatatgg
     3601 cgagaaatac actgatgaaa tattagcaga aatattagat gattgcgatc atgattatat
     3661 atctgggcta agtctattag gtggcgaacc attttgtaat ttggatatta cattaaatct
     3721 tgtcaaagca tttcgagcac gttttggaaa tacaaagaca atttgggtat ggactggatt
     3781 tttatatgaa tatttagcaa atgattgtac agaacgtcga gagttattat catacattga
     3841 cgttttagta gatggtctat ttatacaaca cttattcaaa cctgatttac catataaagg
     3901 ttctttaaat caacgcatta tagatgtaca acaatcactc tcgcatgcgc gtatgattga
     3961 atatatagtt agttgaatat gtattagaag tcaaggtaac attcgttgcc ttggcttctt
     4021 tttaggttag gtacataatt gaaagttaat aaaagcaatt ctttagaaaa atatattgat
     4081 agaatatgac ctaacaatca ttttgatacc aatactaaaa gttgcatatc cgttttttaa
     4141 aaaagttgaa agaaaaaagt ggtactttag tgggaaggaa gtctaacttt ttggtagcgt
     4201 tttacaataa ataaatattc gttaaaaacg tataaatatt cttaaatgcc gtcctagtaa
     4261 aatttgttaa attcgttaaa tcataactta acacagctgt tttagcgcta ttaaagtttt
     4321 gtttattacg agaaaaatta tataaatatt caataattgc caagtttcaa attgtatgaa
     4381 atttgcatta ttattaaata ttagttattg tcaattttgt gaatcaatat aattattaca
     4441 ttttgagata aatcgaaaca ggattcataa aattaataat tagggggagc acaattgaaa
     4501 aaagagaaag ttatggactg gacgaccttt atagggacag tagctgtact tctttttgca
     4561 gttataccta tgatggcttt tccaaaagca agtgaagata tcatcactgg tattaatagt
     4621 gccatttctg attcaattgg ttcgatatat ttatttatgg ggctggcgat tttttgcttt
     4681 gtaatgtata tcgcatttgg taaatatggt aatgtcacac ttggtaaagc aagtgataaa
     4741 ccagaattta atacatttac atgggcggca atgctgtttt gtgcaggcat aggctctgat
     4801 attttatact ggggcgttat tgaatgggct ttttactatc aagttccacc aaatggcgcg
     4861 aaaagtatga gtgatgaagc actccaatat gcgacgcaat atggtatgtt ccactgggga
     4921 ccaattgctt gggctattta cgttttacca gcattaccaa ttggttattt agtgtttgtt
     4981 aaaaaacaac cggtgtataa aattagtcaa gcttgtcgtc cgattttaaa aggtcaaaca
     5041 gataaatttg taggtaaagt tgtagatatc ttatttatct ttggattgct aggtggtgcg
     5101 gcaacatcac tagcgttagg tgtgccatta atttctgcag gcatagaaag attaactggt
     5161 ttagatggta aaaatatgat tttacgttcg gctattttat taacgattac ggtcatattt
     5221 gctattagtt catatacagg attgaaaaaa ggtattcaaa agttaagtga tatcaacgtt
     5281 tggctatcat ttgtactttt agcctttata tttattattg gaccgactgt ttttattatg
     5341 gaaacgacag tgacagggtt cggaaatatg ttgagagatt ttttccatat ggcaacatgg
     5401 ttagaaccat tcggtggtat taaaggtcga aaagaaacga atttcccaca agactggaca
     5461 atattctact ggtcatggtg gttagtatat gcgccattta tcggtttatt tatcgctaga
     5521 atttcaaaag gtagacgcct taaagaagtc gtgctaggaa caattattta tggaacgctt
     5581 ggatgcgtat tattctttgg tatttttggt aactatgctg tgtatttaca aatttctgga
     5641 cagtttaatg taacacaata tttaaataca catggtacag aggcaaccat tattgaagtg
     5701 gtgcatcatt taccattccc atcattgatg attgtactat tcttagtatc tgctttctta
     5761 ttcttagcaa caacatttga ttcgggttca tatattttag cggcagcatc tcagaaaaaa
     5821 gtggtaggcg aaccattacg tgccaatcgt ttattctggg catttgcatt gtgcttattg
     5881 ccattttcat tgatgctagt tggtggcgaa cgtgcattag aagtattgaa aactgcttca
     5941 atactggcaa gtgtgccatt aattgttatt tttattttca tgatgatatc atttttaatc
     6001 attttagggc gcgatagaat taaacttgaa acgcgtgctg aaaaattaaa agaagttgaa
     6061 cgtcgttcat tgcgaatcgt tcaagtatca gaagaagaac aagacgataa tttataattc
     6121 aaataatgat gcataagatt ttgaatttta taagtatata attgattgtc atataaaaca
     6181 aaaacggtaa tttctattta ttgagataga aattaccgtt tatttcgtgg acctattgta
     6241 ttgttttcat catgcataat catcattgcc gctgtccgag gctaatttta attttcagaa
     6301 tcagaaggct gttctggaat tgggaaatat ttgaaaattt caccgctttc aatcgcttcg
     6361 gttaactgtt ctaaccattc gtaataaaca tgtgtatgat caagctgagc tttaattttt
     6421 tgtgcctctt gtgtttcagc ttcagttaaa tcactgcttt caagtaatgg attgataata
     6481 gcttgagcat cttttactgc ttcgacattg atgtcaattt cacgctgaaa ttttttagtg
     6541 aaaaagtttc ggaaaaagat gaaaaagtct ttctcggcga taaaatgttg tttgcggctt
     6601 cctctcgtaa attgttgttt aacaatatca aattcctgca atttcttaac gccagcactc
     6661 atacttggtt tgctcatttg caattgatga cgcatttcat caagcgtcat actgccttca
     6721 aacaccatag tgccatataa gtttcctaca cttctattag tgccatacaa atccattgtc
     6781 tgtccaattg aattaattac aatatctttt gcttgttcta attgttgctg tttgttctga
     6841 gaacgagtca tcattgcacc tccgtacatc attttgttca cgttaaaata aatactaata
     6901 cattataaaa ccttttctaa aaaaagacat taaaaatatt taaagcatta aagttaaatg
     6961 tttcgttaaa taaaaatcta acgaacttac aaaacttaat tcttgagttg ttttgtaaat
     7021 tgacacattt ttcatttcta tgctaacata agtttgtaaa attcgttaaa taaaaattta
     7081 acaaacttaa cggaggttgt tgaatggaac ttttaaaaca tttatctcag cgtcaatata
     7141 ttgatggtga gtgggttgaa agcacgaata aaaatacaag agatattatc aatccttaca
     7201 atcaagaagt gatatttacg gtttctgaag ggacaaaaga ggatgcagaa cgtgcaatct
     7261 tagctgcaag acgtgcgttt gagtctggtg aatggtcgca agaaactgct gaaaaaagag
     7321 gtaaaaaagt acgtgccatc gcggacaaga tcaaagaaca tcgcgaagcg ttagcacgat
     7381 tagaaacatt agatactgga aaaacgttag aagaatcata tgcagatatg gatgatattc
     7441 ataatgtgtt tatgtatttt gctggattag cagataaaga tggtggcgaa atgattgatt
     7501 caccaattcc agatacagaa agcaaaattg ttaaagaacc agtaggtgta gttacacaaa
     7561 ttacaccttg gaattatccg ttattacaag catcatggaa aattgcgcca gcgcttgcta
     7621 cgggttgttc actagttatg aaaccaagtg aaattacacc attaacaaca atacgtgttt
     7681 ttgaattaat ggaagaagtt ggtttcccta aaggaacaat taatcttatt ctaggtgcag
     7741 gttctgaagt tggtgacgta atgtcaggtc ataaagaggt tgaccttgta tcatttacag
     7801 gtggcattga gactggtaag catattatga aaaatgctgc taataatgtt acgaatattg
     7861 ccttggaact tggcggtaaa aatccaaaca ttatctttga tgatgctgat tttgaattgg
     7921 cagtagacca agcgttaaat ggtggatatt ttcatgcagg tcaagtttgt tcagcaggat
     7981 caagaatatt agtacaaaac agtattaaag acaaatttga gcaagcactt attgatcgcg
     8041 tgaaaaaaat caaattaggt aatggttttg atgctgatac tgaaatggga ccagtgattt
     8101 caacagaaca tcgtaataag atcgaatctt atatggatgt agctaaagca gaaggcgcaa
     8161 caattgctgt tggtggtaaa cgtccagata gagacgattt aaaagatggt ctattcttcg
     8221 agccaacagt cattacaaat tgtgatacgt caatgcgtat tgtacaagaa gaggttttcg
     8281 gacctgtcgt tactgtagaa ggctttgaaa ctgaacaaga agcgattcaa ttagcgaatg
     8341 attctatata tggtttagca ggtgctgtat tttctaaaga tattggaaaa gcacaacgcg
     8401 ttgctaacaa gttgaaactt ggaacggtgt ggattaatga tttccatcca tattttgcac
     8461 aagcgccatg gggtggatac aaacaatcag gtatcggtag agaattaggc aaagaaggct
     8521 tagaagagta ccttgtttca aaacacattt taacaaatac aaatccacaa ttagtgaatt
     8581 ggtttagcaa ataaaaatta gataaggtga gtgccattgt aagaacacaa gacactcact
     8641 ttgttttgta taagtggcga aatgttgatt gataatttgg actaaacgca aaatgaatca
     8701 tagattattt catgactgtt agtaacaatc gtaaaaggaa aatcaagtgt tttggttagc
     8761 caagtttagc aattcaacga taaccaatca gccactaaca aatatttcat gcaatactca
     8821 ctttgaaata caacaaactt tggaggtcat aacgatgagt aacaaaaata aatcatatga
     8881 ttatgtcatc attggaggag gcagtgcagg ttctgtacta ggtaatcgtc tgagtgaaga
     8941 taaagataaa gaagtcttag tattagaagc gggtcgcagt gattattttt gggatttatt
     9001 tatccaaatg cctgctgcgt taatgttccc ttcaggcaat aaattttacg attggattta
     9061 ttcaacagat gaagaaccac atatgggcgg tcgtaaagta gcacatgccc gaggtaaagt
     9121 tttaggtgga tcaagttcga ttaatggcat gatttatcaa cgtggtaatc caatggacta
     9181 tgaaggctgg gcagaaccag aaggtatgga aacttgggat tttgcgcact gtttaccgta
     9241 ttttaaaaaa ttagaaaaaa catacggtgc agcgccttat gataaattta gaggccatga
     9301 tggaccaatt aagttaaaac gagggccagc aacgaatcct ttattccagt cattctttga
     9361 tgcaggtgtt gaagcaggtt atcataaaac acctgatgtg aatggattta gacaagaagg
     9421 ttttggaccg ttcgatagtc aagtacatcg tggtcgccga atgtcagctt caagagcata
     9481 tttacatcca gcgatgaagc gtaaaaactt aaccgttgaa acacgtgcct ttgtaactga
     9541 aattcattat gaaggtagaa gagcaactgg tgttacgtat aagaaaaatg gcaaactaca
     9601 taccatcgat gctaatgaag tcattttgtc tggtggggca ttcaatacgc cacaattact
     9661 acaattatct ggtatcggtg attcagagtt cctaaaatca aaaggcattg agccacgtgt
     9721 tcatttacca ggtgtgggtg aaaactttga agatcactta gaggtataca ttcaacataa
     9781 atgtaaggaa cctgtatcat tacagccaag cttagatatc aagcgcatgc catttattgg
     9841 tttacaatgg attttcacac gtacaggtgc agcagcatct aaccattttg aaggtggcgg
     9901 atttgtacgt tcaaataacg aagttgatta tcctaactta atgttccatt tcttaccaat
     9961 agctgtaaga tatgatggtc aaaaggcagc ggtcgcacat ggttatcaag ttcacgtagg
    10021 accaatgtat tcaaactctc gtggtagctt gaaaatcaaa tctaaagatc cattcgaaaa
    10081 accaagtatc cgctttaatt atttatcaac agaagaagat aaaaaagaat gggtagaagc
    10141 aattcgtgta gcaagaaata tcttatctca aaaagcaatg gatccattca atggtggaga
    10201 aatttcacca ggaccagaag tgcaaacaga cgaagaaatt cttgattggg ttcgtagaga
    10261 tggtgaaaca gcattacacc catcttgtag tgctaaaatg ggtcctgctt cagatccaat
    10321 ggcagtagtt gacccgttaa caatgaaagt ccacggtatg gaaaatttac gtgtagttga
    10381 tgcatccgct atgccacgta cgacaaatgg caatatacac gcacctgttt taatgttagc
    10441 tgaaaaagca gcagacatta tacgtggcag aaagccttta gagccacaat atattgatta
    10501 ttataagcat ggtgttcatg atgaaaatga aggtgcaatc gaagtaaaac catacgctaa
    10561 ataaatttat atagtagact cttagtctgg catctattgt attggtgcca gacttttttg
    10621 cgttttatct aaatgagaag ttaatgtgtt gtttaaaaac ggtgtgtgag taagtgaact
    10681 gaaaatagag ttaattgtac attgtgatga ttaattgacc taattggcag acattaaaag
    10741 agtgtacaac cttaaaaagt tcactaaata gacatttaat tgtgttttct tgaaagcttt
    10801 cgtggtagga tttatataag taaattgaga gaaggaatac tatgagcttt tacgaatata
    10861 tacaaacatt taaagatgat aaaacaccat taggcgaatt agcgatttgg attaaagaag
    10921 atgattcatt cccaaaacaa gagaagctaa cagaaaacat tttgtcttat tttcatcaaa
    10981 tgtccaatat agatcatgag tttttagaaa ttgtaaaaag atcactttct ctgtatgatc
    11041 aattaaaatc gtaaaatcag aaataaagtt agctaaaata aaaggccggt gatttcacag
    11101 atgaagttgt cggctatttt catgactaat gttaaataat gatcatgaaa ttgaagacac
    11161 aaaaatagtg ggcgactcct gtccccattt gcatgaaata ttaatacttt ttacaaagtc
    11221 ttatctttta aaaagaagcc aaac
//
LOCUS       DAOBBH010000078        11153 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.078, whole genome shotgun sequence.
ACCESSION   DAOBBH010000078 DAOBBH010000000
VERSION     DAOBBH010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11153
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.078"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            154..960
                     /locus_tag="P6L01_001460"
     CDS             154..960
                     /locus_tag="P6L01_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDP3030532.1"
                     /translation="MKIGTIADLHIDRHNKKTSEDYLEALVEIVKYKKLDILLIAGDI
                     SNHYQLTHQFITQLTKQLDIPVKFVPGNHDLWEVESMTTQDIWNNYKSMSQCLVGKPF
                     IVNEEWAIIGHTGWYDYSFAAQRFSLEHLQKGKHYGATWQDKERISWGISDQNLSKIA
                     AEQVKKDILEVGNRRVILVTHVVTHPDFIVPMPHRIFDFYNAFIGTSDFNPLYAMFDI
                     PYSIMGHVHFRKSVMDDGRCYLCPCLGYPRQWRSEDIYQEINETIQIIEI"
     gene            complement(1023..1679)
                     /locus_tag="P6L01_001461"
     CDS             complement(1023..1679)
                     /locus_tag="P6L01_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDP3030533.1"
                     /translation="MNRKILPVKPISQLFPIPMVMGCEGVSAAMMLQYNNQHIPATEI
                     MRHWPTHPNNPHKGYVGHHLFIKLGNYHQTIFPEAYVPFLQKYNPNIVDGTGKSLEDL
                     KGIIDQGQPVLIYHTNLGSKPLLRVFRFDNKPAKQVSNIHVTLLIGYDDYYYYYIDPL
                     WSHIRRGLVLPAIIPNRKQIIKIRKEKMEYSFNSPGRKCIYVQPHSYTIENQQQNKHT
                     "
     gene            1792..2265
                     /locus_tag="P6L01_001462"
     CDS             1792..2265
                     /locus_tag="P6L01_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDP3030534.1"
                     /translation="MEYQLQQLASLTLVGIKETYENGRQAQQHIAGFWQRCYQEGVIA
                     DLQLKNNGDLAGILGLCIPELDGKMSYMIAVTGDNSADIAKYDVITLASSKYMVFEAQ
                     GAVPKAVQQKMEEVHHYIHQYQANTVKSAPFFELYQDGDTTSEKYITEIWMPVKG"
     gene            complement(2388..3599)
                     /locus_tag="P6L01_001463"
     CDS             complement(2388..3599)
                     /locus_tag="P6L01_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HDP3030535.1"
                     /translation="MQSTKTKTKHFSFLLLITLGVMTAFGPLTIDMYVPSLPKVQGDF
                     GSTTSEIQLTLSFTMIGLALGQFIFGPLSDAFGRKRIAVSILIIFILVSGLSMFVDQL
                     PLFLTLRFIQGLTGGGVIVIAKASAGDKFSGNALAKFLASLMVVNGIITILAPLAGGL
                     ALSVATWRSIFTILTIVALIILIGVASQLPKTSKDELKQVNFSSVIKDFGSLLKKPAF
                     IIPMLLQGLTYVMLFSYSSASPFITQKLYNMTPQQFSIMFAVNGVGLIIVSQVVALLV
                     EKLHRHILLIILTIIQVVGVALIILTLTFHLPLWVLLIAFFLNVCPVTSIGPLGFTMA
                     MEERTGGSGNASSLLGLFQFILGGAVAPLVGLKGEFNTSPYMIIIFITAILLVSLQII
                     YFKMIKKQHVA"
     gene            complement(3781..4440)
                     /locus_tag="P6L01_001464"
     CDS             complement(3781..4440)
                     /locus_tag="P6L01_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030536.1"
                     /translation="MKRTDKYRDSYQYDNQNQNHRRQSEDASYRQQYAKGDPEEHPER
                     YYNGRDYRREQILEEENEKSRRSKKWLYIIIAILLIIVAIFVTRALLNNDSDKVSNDP
                     KVSQNYKKQVENQDGQINQQVDNAKENIKNNQKTDDIIKNLQNQIDNLKQQEQNKADS
                     KLTQFYQDQINKLTEANNALKNNASQGKIESMLNDINTKFDSIKSKLESLFKDDNGGA
                     N"
     gene            complement(4500..5642)
                     /locus_tag="P6L01_001465"
     CDS             complement(4500..5642)
                     /locus_tag="P6L01_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDP3030537.1"
                     /translation="MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGNTLHYDIIP
                     MADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDL
                     LEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVKFTDV
                     NGDLLQMNGANLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGA
                     DAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVV
                     FDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENY
                     QHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ"
     gene            5910..6296
                     /gene="gtxA"
                     /locus_tag="P6L01_001466"
     CDS             5910..6296
                     /gene="gtxA"
                     /locus_tag="P6L01_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase GtxA"
                     /protein_id="HDP3030538.1"
                     /translation="MKLTQTHAEILKFIIVGGINTLNYYVVYLLLLKLLHIEYMISHI
                     TGFLVAFVISYYLNCYFVYRVKPTWRKFISFPITQIVNVSLQTVLLYVFVSWLNLPAE
                     IAPFAGLVITIPITFVLSKWILKDSN"
     gene            6430..6537
                     /locus_tag="P6L01_001467"
     CDS             6430..6537
                     /locus_tag="P6L01_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDP3030539.1"
                     /translation="MFNLLINIMTSALSGCLVAFFAHWLRTRNNKKGDK"
     gene            7240..9003
                     /locus_tag="P6L01_001468"
     CDS             7240..9003
                     /locus_tag="P6L01_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3030540.1"
                     /translation="MNNQTNWIKILSGFASDSKWKIMLSILLSIISVFSGLVPYWAVF
                     KIILMMINNAYTINSIMVYIFIALITYISQVCCFGASTMLSHITAYEILSEIRKKLAQ
                     KLMRLPLGVVESKKIGELKNIFVDKVETIELPLAHMIPEVIGNLLLSAAIFLYIMLID
                     WRMASALLVTIPISIFAFKKVMSGFNETYAEQMKSNNYMNSAIVEFIEGIEVIKTFNQ
                     SQSSYKKYKDAVDNYKIHTLNWFKNTWGYMNLGASVLPSTFLGILPVGMYLISINQLN
                     YAEFFLCIVLSLGVVAPIKNFTNYVNHLKSIQYALTEVNQILSLEELVLSTKFKKPQH
                     YEIAFNNVGFSYNKDKDDLVFKHLSFTVPENNFTAIVGASGSGKSTIAKLISRYWDVT
                     SGEITIGGINIKDIESKQLNDLVGFVGQDNFLLNLTFKENIKLGNPEATDEAVEKAAK
                     LAQCHEFIEKLPDGYDTNVGTVGDKLSGGEKQRVTIARMILKDAPIIVLDEATAYVDP
                     DNEQKIQEALNVLTQDKTLIVIAHRLSTIQHADQIIVLGKQQILEKGSHHLLLKLNGN
                     YKKMWDTHMHTKDWGINTGHN"
     gene            9028..10761
                     /locus_tag="P6L01_001469"
     CDS             9028..10761
                     /locus_tag="P6L01_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3030541.1"
                     /translation="MFQITFKILNWIRPYKARMILGFSMSFLNAIFIALPIFLAAKIF
                     NNVLSHKPIYMKDILNVVIIMVLLVIGRFITAYFKSKSHESIAYEMSAKERLDIGDKL
                     KNVRLGYFNSHHSNELTTIVTTDLTFLENFAMKMVDIVVNGYILITVLILSLLVVSWQ
                     VSLLACIGVLLSFFAIQLLERKSRQNAPAYHNVQNQLVEKVLEVIRGIQVIKSFAKEN
                     TSLKSFNQAVNESKRINTKIEMQYIPFNLLHLLSLKVVSIMIVLVACLLYMNHSIDLP
                     TLIMISIFSFVIFDSVENINSAAHVLEMIDMTIDDIEKIKNAPELDENGKNLTIKNEN
                     IAFQNVNFSYDDKQVIKNVNFEIPTQTSTAIIGPSGSGKSTLCHLLLRFYDIDDGNIR
                     IDGVDIKDMTLSTLMSKISAVFQKVYLFNDTIENNILFGNPGATKEEIIRAAKQACCH
                     DFIMSLPEGYQTMLNEKGSNLSGGEKQRISIARAILKDAPIIILDEATASIDPENEQL
                     IQTAINELSKGKTVITIAHKLETIKNADQIIVLNEGEIIQKGSHDELIRKPGMYQDFI
                     RIKSKSAGWKL"
     gene            10992..11153
                     /locus_tag="P6L01_001470"
                     /pseudo
     CDS             10992..11153
                     /locus_tag="P6L01_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501179.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3846 a         1619 c         2032 g         3656 t
ORIGIN      
        1 gtatgataac taaaacttat cgattttttg aaaagatata agtttttatg caaaaatata
       61 aaataatcat ctaaatgaag cagttaacta tatatattag ataagctatg gcataataaa
      121 taacatttta atttgagtaa agaaggtatg ggtatgaaga tagggacaat cgccgattta
      181 catattgatc gtcataataa aaaaacatcc gaagattact tagaggcatt ggttgaaata
      241 gttaagtata agaaattgga tatattgttg attgcgggag atatctcaaa tcattatcaa
      301 ttgacgcacc aatttataac gcaattgaca aaacaattag atatacctgt gaaatttgtt
      361 cctggaaacc atgatttatg ggaagttgaa agtatgacta cgcaagacat ttggaataat
      421 tataagagca tgtcacagtg cttggtagga aaaccattta tagtaaatga agaatgggca
      481 ataataggac atacaggttg gtatgattat agctttgcag cacaacgatt ttcattagaa
      541 catttacaaa aaggaaaaca ttatggtgcg acttggcaag ataaagaacg aatatcttgg
      601 ggcatatcag atcaaaattt atctaaaata gcggctgaac aagtgaagaa agatatatta
      661 gaagtaggaa atagacgagt gattttagtc acacatgttg tgacgcaccc tgatttcatt
      721 gttcctatgc cacatcgtat attcgatttt tataatgcat ttattgggac aagtgatttc
      781 aatcctttgt atgcgatgtt cgatatacca tatagtatta tgggccatgt tcattttcgt
      841 aaaagtgtga tggatgatgg cagatgttat ctctgtccgt gtctaggcta tccaagacaa
      901 tggcgttcag aagatattta ccaggaaata aatgagacga tacaaataat agaaatttaa
      961 aatgcgcaaa cctgacccag tttgcgcatt ttatgtttta cacacgcgag taatgtgttt
     1021 acttacgtgt gtttattttg ttgttgattt tcaattgtat atgaatgtgg ttgcacataa
     1081 atgcattttc ttcctggtga attaaagctg tattccattt tctctttacg aattttaata
     1141 atttgtttgc gatttggaat gatggcaggt aaaactaggc cacgacgaat atgactccaa
     1201 agtggatcga tataatagta atagtaatca tcgtacccaa tgagtaacgt tacgtgaata
     1261 tttgaaactt gtttcgctgg cttgttatca aagcggaaaa cacgtagtaa tggtttagag
     1321 ccaagattag tatggtatat taacacaggt tgaccttgat cgataatacc tttaagatct
     1381 tctaaggatt taccagtgcc gtctacgata ttaggattgt atttttgtaa aaatggtaca
     1441 tatgcttctg gaaatatcgt ttgatgataa ttgccaagct taatgaataa gtgatgtcca
     1501 acataacctt tatgtggatt gttcggatgt gtcggccaat gtctcataat ttctgtagca
     1561 gggatatgtt ggttattgta ttgcaacatc atggctgcgg aaacaccttc acaccccatg
     1621 accataggga taggaaatag ctgactgata ggtttaactg gtaatatttt tcggttcata
     1681 atatagtcct cgcattgatt caataaatat ttaatataat tatatagcgt caatgcaaaa
     1741 tgtcctaaac atatgtttta catgagtgaa taaaattaat ggagtgataa aatggaatat
     1801 caattacaac aacttgcgtc gttaacgtta gtaggtatta aagaaacgta tgaaaatgga
     1861 cgacaggctc agcaacatat agccgggttt tggcaaaggt gttatcaaga gggagtaatt
     1921 gcggatttac agttaaaaaa taatggtgat ttagccggga tacttggctt atgtataccc
     1981 gaattagacg gtaagatgtc atatatgatt gcagttaccg gagataatag tgctgatatt
     2041 gcaaaatatg atgtcataac attagcaagt tcaaagtata tggtatttga agcacagggc
     2101 gcagtaccta aagcagttca acaaaaaatg gaagaggttc atcactacat acatcaatat
     2161 caagcaaata cggtaaaatc agcaccattt tttgagttgt atcaggatgg tgatactaca
     2221 agtgaaaaat atattacaga aatttggatg cctgttaaag ggtgattgaa tagaaatgta
     2281 cttgatatga aggcgtaatt ttagttgaag attggatttc ataattcata aagaagaacc
     2341 ccgacaaaga tagatatctt tgctagggtt ctaattatgt tgaagtgtta tgcgacatgt
     2401 tgctttttaa tcattttaaa gtaaatgatt tgtagactga ctaatagaat ggctgtaatg
     2461 aagataataa tcatatatgg tgatgtatta aattcgcctt ttaagccaac taatggtgca
     2521 acagcgccac ctaagataaa ttggaataag ccaagtaaac ttgatgcgtt accactgcca
     2581 cctgttcgtt cttccatagc cattgtgaaa ccaagtggtc caattgacgt cacaggacac
     2641 acatttaaga agaatgcgat gagtaagacc caaagtggta aatggaatgt aagtgtcagg
     2701 ataattaaag caacacccac cacttgtata atagttaaaa tgattaataa tatgtggcga
     2761 tgtaattttt ctactaataa agcaacgact tgactgacaa tgattaaacc tacaccgtta
     2821 acagcaaaca tgatactaaa ttgttggggt gtcatattat acaatttttg agtaataaat
     2881 ggcgatgcag atgaataact aaaaagcatt acataagtta aaccttgtaa tagcattgga
     2941 ataataaatg ctggtttttt caaaagactt ccaaagtctt taatgacgct actaaaattc
     3001 acctgcttta attcatcttt agatgtttta ggtaattgag aagcgacgcc aattaaaatg
     3061 atgagtgcca caatagttaa aattgtgaaa atagaacgcc atgttgctac ggataaagct
     3121 aatccaccag ctaatggtgc aagaatagtg atgatgccat ttactaccat taaagatgct
     3181 aaaaatttag cgagtgcgtt gccactaaat ttatcaccag cagaggcttt ggcaatcacg
     3241 atgacgccac caccagttaa accttgaata aatcgtaaag ttaagaataa tggcaattga
     3301 tcaacaaaca tagacaaacc tgataccaaa atgaaaatga tcaaaatgga tacagcaatc
     3361 cgtttgcgac caaaagcatc ggataaaggt ccaaagataa attggcctag tgcaagacca
     3421 atcattgtga atgataatgt taattgaatt tctgatgtag tagaaccaaa atcaccttgc
     3481 actttaggta atgatggtac gtacatatct atagttagtg ggccaaaagc ggtcatgacg
     3541 cctaacgtaa ttagcaataa aaatgaaaaa tgcttcgttt tggttttcgt tgattgcatg
     3601 atcagactcc tttaaatgat ataattatat aaattatata cgttttgtgt gtatttgtat
     3661 gtagttagaa tacaagttta tataaaaaat ccctatactc ttaatgcatc atcacgaata
     3721 acgtagtgat acagataaag tatagggaat taatgtttat catcaaagca ggtgtaataa
     3781 ttaattagcg ccaccattgt catctttaaa taagctttct aatttagatt taatactgtc
     3841 gaattttgta ttaatatcat ttaacatgct ttcaatttta ccttggcttg cattgttttt
     3901 aagtgcatta tttgcctctg tcaatttgtt gatttggtct tgataaaatt gagttagctt
     3961 agaatcagct ttgttttgtt cttgctgctt caagttgtcg atttgatttt gtaaattttt
     4021 aataatgtca tcagtttttt ggttgttttt aatattttct ttagcattat ctacttgctg
     4081 gttaatttgg ccgtcttgat tttcaacttg ttttttataa ttttgagaga ctttagggtc
     4141 attactaact ttatcgctat cattgttaag taaggcgcgt gtgacaaaaa tagcgacaat
     4201 aattaagaga atggcaataa tgatatataa ccattttttt gaacggcggg atttctcgtt
     4261 ttcttcttca agaatttgtt ctcttcgata atctctacca ttatagtatc gttccgggtg
     4321 ttcttcagga tcgcctttag catattgttg tctatacgat gcgtcttcag attgacgacg
     4381 atgattttgg ttttgattgt cgtattgata tgaatcacgg tatttatcag ttcttttcat
     4441 gggtttcacc tcattcaagt tttaactgca aataataaag ctgatgattt actttgacat
     4501 tattgtaatt tcagaatacg agcaatgtca gttgcagtgt ttaataaatt ttgttcgcta
     4561 ttttgtagga catcttctaa agtgctaggt gaagagatta tagaataggc actatcaata
     4621 ccgaaatcgt aaacatgttg ataattttcg cctagactgc cacaaatcgc gatgacagga
     4681 atatgatatt gttttgcagc taacgcaacg cctacgggtg ttttaccaaa gatggtctga
     4741 taatccatgc gtccttctcc agtaataacg aggtctgcat ctttaattct ttgatgaaaa
     4801 tctgtaatgt caaagacgac atcaatacct tttgttaaag ttgtctcaca aaacgctaat
     4861 aatgctgcgc ccataccgcc agctgcacca gaacctggta tttgattaac ggactttcct
     4921 gtgcacattt ttatcttatc atgataatgc gacattgcga aatccaactt tggtatcatc
     4981 tttgcatcag cgcctttttg aggaccataa atataggtag caccattttc acccaataaa
     5041 ggatttgaaa catcacaggc cactttaaag gtcacctctt ttaatcgcga atctagattg
     5101 gttatatcga tttgtgcaat gtgagcaaga ttagcaccat tcatttgtaa taagtccccg
     5161 tttacatcag taaactttac gcctagtgca cttagcatac ctgtaccacc atcatttgtt
     5221 gcactgccac caatccctaa aataatggtc ttagcaccat gatttaatgc atctttaatt
     5281 agttcaccgg taccatatga tgatgtgtat aaaggattac gttcctcttt ttctaataaa
     5341 tccaaacctg acgctgccgc catttcaata attgcaattt gttgttcgtc tgcgcgtgca
     5401 taacatgctt caataggtcg cattaaaggg tcattaacga tgactgtata cttagtggca
     5461 cctgttgcat gcattaaagc atctgtggta ccttcaccac catcagccat cggaatgata
     5521 tcataatgaa gggtattccc ataaacatta gtaaatgcct gttttataat attgccaact
     5581 tgctgtgcgg tcatactttc cttaaatgag tcaggagcga tgacaatttt ttgtaattgc
     5641 atagtagaca tcctctcatt actgttgtcg ctcttattgt atataaaatt actagtgaac
     5701 taaagtaatc tctatagtgc taatgagtca cgcgataaat cagttacatt ataatggcgt
     5761 ataaggatga gaggaaagag actaaatttg ctgtgaaagg gtataaaggt tgaaataagt
     5821 aaatttgaag tgaagcggat tggctcgcat attcgagtat gttggagaac gtagctatgc
     5881 atatattttg atgttgagga gttacacata tgaaactaac acaaacgcat gctgaaattt
     5941 taaaatttat cattgttggc ggcattaata cgttaaatta ttatgttgtc tatttattgc
     6001 tgttaaaatt attacacatt gaatatatga ttagtcatat taccggattt ctagtcgctt
     6061 ttgtgatttc gtattatttg aattgttatt ttgtttacag ggtaaaacct acttggagaa
     6121 aattcattag ttttccaatt acgcagattg tcaacgtaag cttacaaaca gttttattat
     6181 atgtttttgt atcctggcta aatttgccag cggaaattgc gccatttgca ggtttagtga
     6241 ttacgatacc catcacattt gtattatcta aatggatttt aaaagatagt aattaaagta
     6301 gcgtaaacca ctaaaaaacg ctttactcga actttaatat tgcgtgaaat ataataaata
     6361 aatgatataa tataatggtc gcctaacatc tcgggcgtta ctttaacgca gagaggaggt
     6421 gtatgatgga tgttcaattt attaattaac atcatgactt cagctttaag cggctgtctt
     6481 gttgcgtttt ttgcacattg gttacgaacg cgcaacaata aaaaaggtga caaataacca
     6541 acacctaaat aaatcccctc actatggcag tagtgagggg atttggtgta tttagatgtt
     6601 caatttatta tgtaattaat tatagcattg ataaacaagg tgtgcaatgt attttgcaat
     6661 attacaataa atgaatttaa ttatattaga attaagtatt tcgaaaaatg atttaatagt
     6721 tgagtcatag gatcattaga taaagcgaat aatcaattat agggacattg ggatacaaat
     6781 caatgtacta gactctacaa ggctatactc agggacccaa catagagaat ttggatccac
     6841 agtttctaca gacaatgcaa gttggcgggg ccccaacaca gtagctggcg gaaagtcagc
     6901 ttacaataat gtgcaagttg gcggagcccc aacatagagt tttactcatg tattcctatt
     6961 tttaagtaca tattagctgt ggctaaggat aaagaatcat tacataataa atcattagtg
     7021 gctctttatc atttctgccc cataaccttg tgaattttta aaagaaatgt gtcacaaatc
     7081 cacgcgacat tgataatcgt tatcaactaa tatatgatgt acttgtaata agtattaatt
     7141 tttaacaaat gacaaattta tattatgtat atcatccagt gaaataaata atataagcgg
     7201 cttaatttag attattaatt gaaaggagtt gcatatacga tgaacaatca gacaaattgg
     7261 ataaagatac tcagcggatt tgcttctgat agtaaatgga aaattatgtt atcaattttg
     7321 ttgtctatca tcagtgtctt ttctggatta gttccatatt gggcagtctt taaaattatt
     7381 ttaatgatga ttaacaatgc atatacgatt aattcgatta tggtttatat ctttattgct
     7441 ttaatcactt atatttcaca agtgtgttgt tttggagcgt caacgatgct atcacatatt
     7501 acggcatatg aaattttatc tgaaattcgt aaaaaattag ctcaaaaatt aatgcgcctc
     7561 cctttaggcg tagtggaatc taagaaaata ggtgaattaa aaaatatatt tgtcgataag
     7621 gttgaaacaa tagaattacc tttagcacat atgattcctg aagttatagg aaacttactt
     7681 ttgtcagctg ctatcttttt atacataatg ctcattgatt ggcgtatggc tagtgcctta
     7741 ttagtaacga taccaatttc aattttcgct tttaaaaaag ttatgtctgg atttaatgag
     7801 acatatgctg aacaaatgaa atcaaataat tatatgaata gcgcgattgt agagtttatc
     7861 gaaggcattg aagtgattaa aacatttaat caatctcaaa gttcatataa aaaatataaa
     7921 gatgcagtag ataattataa gattcacact ttgaattggt ttaaaaatac atgggggtat
     7981 atgaatttag gagctagtgt attaccttca acatttttag ggattttacc ggtcggcatg
     8041 tatttgatat ctatcaacca attaaactat gcggaatttt tcctatgcat cgtattatct
     8101 ttaggtgtag tagcaccgat taagaatttt actaattatg taaatcattt aaagtctata
     8161 caatacgcat taactgaagt gaatcaaata ttaagtctag aagagttagt attgtcaaca
     8221 aaatttaaga agcctcaaca ttatgaaatt gcttttaata atgttggatt ttcatataac
     8281 aaagataagg acgatctggt ctttaagcat ttatcattta cagtaccaga aaataatttc
     8341 acagctatcg tcggggcttc tggtagcggt aagtcaacca ttgccaagct tatatcacga
     8401 tactgggatg tgacttcagg tgaaattacg attggcggta taaatataaa agatattgaa
     8461 tcgaaacaac ttaacgattt agttggtttt gttggacaag ataacttttt attaaatctt
     8521 acttttaaag aaaatattaa acttggcaat ccagaagcta cggatgaggc agttgaaaaa
     8581 gctgccaagt tagcacaatg tcatgaattt attgaaaagt tgccagatgg atatgatacg
     8641 aatgttggta cagtgggaga taaattgtct ggtggtgaaa aacaacgagt cactattgca
     8701 agaatgatat taaaagatgc gcctatcatt gtattagatg aagcgactgc ttacgttgat
     8761 ccagacaacg aacaaaaaat tcaagaagca ttaaatgtgt tgacgcagga taaaacattg
     8821 attgttattg cacatcggtt atctacgatt caacatgcag atcaaattat tgttttaggt
     8881 aaacaacaaa ttttagaaaa agggtcacat cacttattgc taaaattaaa cggtaattat
     8941 aagaaaatgt gggatacgca catgcataca aaagattggg gaatcaatac tggacataat
     9001 taaactaatg agaaaaggag gtcaaatatg tttcaaatta catttaaaat tttaaactgg
     9061 ataagaccat ataaagcaag aatgatttta ggatttagca tgtcattttt aaatgctatt
     9121 tttattgcgt tacctatctt tttagctgca aaaatattta ataatgtgct atctcataaa
     9181 cctatttata tgaaagatat attgaatgtt gtaattatca tggttttatt agtgattgga
     9241 cgatttatta cagcatattt caaaagcaaa agccatgaga gcatcgctta tgaaatgagt
     9301 gcgaaagaac gtttagatat aggggataaa ttgaaaaatg taaggttagg ttattttaat
     9361 tcgcatcatt caaatgagtt aacaacaata gtaacaacag atttaacatt tctagaaaac
     9421 tttgccatga aaatggtaga tattgttgtt aatggataca tattaattac agtactcata
     9481 ttgtctctac ttgtagtttc gtggcaagta tcattattag catgcattgg cgtattacta
     9541 tcattttttg cgattcaatt attagaaaga aagagtcgac aaaatgcgcc agcgtatcat
     9601 aatgtacaaa accaattagt ggaaaaggta ttggaggtta ttcgtggtat tcaagtaata
     9661 aaatcattcg cgaaagaaaa tacgagtctt aaaagtttta accaagcagt caatgaaagt
     9721 aaacgtataa atacaaaaat agaaatgcaa tatatcccat tcaatttatt gcatttactt
     9781 agtttaaaag ttgtttcaat aatgattgta ttagttgcat gcttattata tatgaatcat
     9841 agtattgatt tacctaccct tattatgatt tcaatttttt catttgtgat atttgatagt
     9901 gttgaaaata ttaatagtgc tgcacacgta cttgaaatga tagatatgac gatagatgat
     9961 attgaaaaga taaaaaatgc tccagaactg gatgagaatg gaaaaaattt gacgattaaa
    10021 aatgaaaata tcgcttttca aaacgtgaac ttttcatatg atgataaaca agtgataaag
    10081 aatgtgaatt ttgagatacc tacacaaaca tcaacagcaa taattggacc ttcaggaagt
    10141 gggaaatcta cattatgtca cttactcttg cgcttttatg atatcgatga tggaaatatt
    10201 cgcatcgatg gtgttgatat taaagatatg acattaagta cgttaatgtc gaaaattagt
    10261 gcagtatttc aaaaggtgta tttatttaat gatacgattg aaaataacat attgtttggc
    10321 aatccaggtg caacgaaaga agaaattatt cgtgcagcga agcaagcatg ttgccacgac
    10381 ttcatcatgt cattacctga gggatatcaa acaatgctaa atgaaaaagg tagtaattta
    10441 tctggcggag aaaagcaaag gatttctatt gctagggcga tattaaaaga tgcaccaata
    10501 attattttag atgaagcaac tgcaagtatt gaccctgaaa atgaacagct gattcaaacg
    10561 gcaattaatg aattaagtaa aggcaaaaca gtaattacaa ttgcacataa acttgaaact
    10621 attaaaaatg cagatcagat tatagtgctc aatgaaggtg aaataattca aaaaggtagt
    10681 catgatgaat taattcgaaa accaggaatg tatcaagact ttataagaat aaaaagtaaa
    10741 tcagcaggct ggaaattata aataatagtt taatatggaa tcagaatttg gaaataaaaa
    10801 gggtgataga taatcaacag caaaataaat cccctcacta gggcaatagt gaggggattt
    10861 ggtatattta gatgttcaat ttattatgta actaattata gcattgatga acaaggcgct
    10921 caacgtaata ttacaattaa tggattgttt cacaacatgg taaagctagg atgtcttttt
    10981 gttaagaaat aatacaatta ttaattttaa ctgacatatt ttaacgaatc gctgtttata
    11041 tctgcccaaa tttcgtaaga agccttagtc atttcaatag caaggtcaaa attttgccaa
    11101 aaattatagt tatactgaga agtttaaatt catgttttct tgaaaaacat gcg
//
LOCUS       DAOBBH010000079        11108 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.079, whole genome shotgun sequence.
ACCESSION   DAOBBH010000079 DAOBBH010000000
VERSION     DAOBBH010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11108
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.079"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(22..984)
                     /locus_tag="P6L01_001471"
     CDS             complement(22..984)
                     /locus_tag="P6L01_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HDP3030542.1"
                     /translation="MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVA
                     DTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFEKAL
                     QQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEG
                     LDDKEAKQKAEEIQKEVSKDPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEK
                     ALFKLKDGEVSEVVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAY
                     KDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGAQGGQSGMSQ"
     gene            complement(1189..1746)
                     /locus_tag="P6L01_001472"
     CDS             complement(1189..1746)
                     /locus_tag="P6L01_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HDP3030543.1"
                     /translation="MFLCKRQIDINARFGLPRIAFMSAVATIIMFLVSYEVMYFLSNT
                     PLSDRHFLIFLLLVFMTYPLHKSIHLLFFLPYRKSFKVHKLTKRKWLIFYNTYVNQPV
                     HKFYFCINLILPLIILSAMFVYLTISFPQYGHYFMFLLALNFGISITDLLYLKIIIFS
                     NYGQYIEEHSTGINILKKIKNPYHL"
     gene            complement(2481..2846)
                     /locus_tag="P6L01_001473"
     CDS             complement(2481..2846)
                     /locus_tag="P6L01_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDP3030544.1"
                     /translation="MKASRILFGIGVGVAAGFVVALQGRDDKSVKNNTIDRTAPTGSK
                     SELQREFETIKQSFNDILNYGVQIKNESAEFGSSIGGEIKSLLGNFKSDINPNIERLQ
                     SHIENLQNRGEDIGNEISK"
     gene            complement(2988..3410)
                     /locus_tag="P6L01_001474"
     CDS             complement(2988..3410)
                     /locus_tag="P6L01_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDP3030545.1"
                     /translation="MSETIFGKILTGEIPSFKVYEDDYVYAFLDISQVTKGHTLLIPK
                     KASANIFETDEETMKHIGAALPKVANAIKRAFNPDGLNIIQNNGEFADQSVFHIHFHL
                     IPRYENDIDGFGYKWETHEDILDNDAKQQIAEQIQAQF"
     gene            3544..4284
                     /locus_tag="P6L01_001475"
     CDS             3544..4284
                     /locus_tag="P6L01_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3030546.1"
                     /translation="MTVKVEQLTGGYGKRPVIKDINFELNKGEIVGLIGLNGAGKSTT
                     IKHMLGLLTPMEGSLSISDININDDIEAYRRKLSYIPESPVIYEELTLEEHIEMTAMA
                     YDIDRDEAMNRAMPLLKTFRLENELKVFPSHFSKGMKQKVMIICAFIVNPELYIIDEP
                     FLGLDPLGIQSMLDLMVEKKNEGRTVLMSTHILATAERYCDRFIILDEGEVVAFGDLE
                     ALRQQTGLHNQTLDDIYIHVTQGGDVHA"
     gene            4277..5500
                     /locus_tag="P6L01_001476"
     CDS             4277..5500
                     /locus_tag="P6L01_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3030547.1"
                     /translation="MHNQATTLFNKRLHALRKEKNYYNKFIFNGHFMVFLLILLGAFI
                     FGYGEWLKHIPTNINFALIAAVIVALTSIFPMRPLLKEADKIFLLPFEKHMSQFMRHA
                     ILYSYFARILIQLIIVIVMFPLFYNINQHNVAFYICFGFSALIFPYVGLRLRWQWYQS
                     GLKTWQVNLISFIIFALTYYLLLAPKWYIVFVMVALPVLIEFLVKKYKPGFLYPWEKM
                     IAIEHRHHMNYYKFVNMFTDVKHLKESAVRRSYLDILLPVPKGSKFNSNAMYLFLFIR
                     SFIRGRDAFNIIFRLVIIAVLLMVWLSYPLVTGVIGSLFVYIILLQMAQFYSQQAYGL
                     WPQVWPVPEEKVIKGYEQFLYRLMFVICTVFAVTFIIKHMTLFYVVLIFYIVGLLTIR
                     SIIKKLKYQETLLRD"
     gene            complement(5624..6127)
                     /gene="traP"
                     /locus_tag="P6L01_001477"
     CDS             complement(5624..6127)
                     /gene="traP"
                     /locus_tag="P6L01_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein TRAP"
                     /protein_id="HDP3030548.1"
                     /translation="MKKLYTSYGTYGFLHQIKINNPTHQLFQFSASDTSVIFEETDGE
                     TVLKSPSIYEVIKEIGEFSEHHFYCAIFIPSTEDHAYQLEKKLISVDDNFRNFGGFKS
                     YRLLRPAKGTTYKIYFGFADRHAYEDFKQSDAFNDHFSKDALSHYFGSSGQHSSYFER
                     YLYPIKE"
     gene            6290..6400
                     /locus_tag="P6L01_001478"
     CDS             6290..6400
                     /locus_tag="P6L01_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030549.1"
                     /translation="MIKCQFYDIYLLKIRTNERYDNIDKNDFNLGGLYGA"
     gene            6390..7427
                     /gene="hemE"
                     /locus_tag="P6L01_001479"
     CDS             6390..7427
                     /gene="hemE"
                     /locus_tag="P6L01_001479"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDP3030550.1"
                     /translation="MVHNKNNTILKMIKGEETSHTPVWFMRQAGRSQPEYRKLKEKYS
                     LFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPI
                     KTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSK
                     NYNFTKAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYR
                     RYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQQL
                     GVTKTLQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRK
                     VSEFVHTYTQR"
     gene            7485..8408
                     /gene="hemH"
                     /locus_tag="P6L01_001480"
     CDS             7485..8408
                     /gene="hemH"
                     /locus_tag="P6L01_001480"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDP3030551.1"
                     /translation="MTKKMGLLVMAYGTPYKESDIEPYYTDIRHGKRPSEEELQDLKD
                     RYEFIGGLSPLAGTTDDQADALVSALNKAYADVEFKLYLGLKHISPFIEDAVEQMHND
                     GITEAITVVLAPHYSSFSVGSYDKRADEEAAKYGIQLTHVKHYYEQPKFIEYWTNKVN
                     ETLAQIPEEEHKDTVLVVSAHSLPKGLIEKNNDPYPQELEHTALLIKEQSNIEHIAIG
                     WQSEGNTGTPWLGPDVQDLTRDLYEKHQYKNFIYTPVGFVCEHLEVLYDNDYECKVVC
                     DDIGANYYRPKMPNTHPLFIGAIVDEIKSIF"
     gene            8432..9832
                     /locus_tag="P6L01_001481"
     CDS             8432..9832
                     /locus_tag="P6L01_001481"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HDP3030552.1"
                     /translation="MTKSVAIIGAGITGLSSAYFLKQQDPNIDVTIFEASNRPGGKIQ
                     SYRKDGYMIELGPESYLGRKTIMTELAKDIGLEQDIVTNTTGQSYIFAKNKLYPIPGG
                     SIMGIPTDIKPFVTTKLISPLGKLRAGLDLIKKPIQMQDGDISVGAFFRARLGNEVLE
                     NLIEPLMGGIYGTDIDKLSLMSTFPNFKEKEEAFGSLIKGMKDEKNKRLKQRQLYPGA
                     PKGQFKQFKHGLSSFIEALEQDVKNKGVTIRYNTSVDDIITSQKQYKIVYSNQQEDVF
                     DGVLVTTPHQVFLNWFGQDPAFDYFKTMDSTTVATVVLAFDEKDIENTYDGTGFVIAR
                     TSDTDITACTWTSKKWPFTTPEGKVLIRAYVGKPGDTVVDDHTDNELVSIVRRDLSQM
                     MTFKGDPEFTIVNRLPKSMPQYHVGHIQQIRQIQAHIKQTYPRLRVTGASFEAVGLPD
                     CITQGKVAAEEVIAEL"
     gene            10155..10709
                     /locus_tag="P6L01_001482"
     CDS             10155..10709
                     /locus_tag="P6L01_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HDP3030553.1"
                     /translation="MTDVIIVHSMHGNSRNHWYQWLEHNLTLEGYDVTLFNFESPEAK
                     TVDQWIEAMTKQINVRKKDTYFVTHGLGSITALKYIEMIDQPIEGFFSIAGFKEDAEN
                     IDLDIDLSNVTIDYDNIKKKVDNFYGLSSKNDKYVSYKETQRLMNALEGNLRIVEDGG
                     HFLEEDGFETFTALQDRMQDYMTR"
BASE COUNT         3836 a         1560 c         1951 g         3761 t
ORIGIN      
        1 ccacggtcgc tcggttttgt gttattggct catgccggat tgtccgcctt gtgcgccacc
       61 ttgtttaagt ttttcagggt ttaagatttt atcttcgaca actgatttaa tatcacgatc
      121 tttaaagtca acatcgtatt cttttaatag atctttgtat gcatcagtta ataattttgg
      181 atttttttgt actttctgat cgactaattt ttctttcagg ctttgttttt cactgttaaa
      241 gtctgttggt ttatcagctt taataatatg atatccaaag cttgatttaa caacctctga
      301 tacttcacca tctttaagct taaatagtgc tttttcaaaa tctttatcag tttgtccttt
      361 aagaacataa cctaattcgc catctttttt agctgaacca gtatccattg attctttttt
      421 agcgatttca ccaaatttac ttggatcttt tgaaacttct ttttgaattt cttcagcttt
      481 ttgtttcgct tctttatcgt ctaagccttc tttgtcgctt ttcttagatt taactttaat
      541 taaaatgtgt gaagctttct tgctgtcttc tttaatttca gaatcagaga ttttaatttt
      601 atctgatagt aattctttat gataagcagc agtacgtaaa ttttctttat atttatcggc
      661 tgttaaacct tgctgttgaa gggccttttc aaatttatct ttaccgccgt attgcttttg
      721 cattttttca atttgttcgt caatcttctt atcattaact ttatttttat atttatcagc
      781 taaaatttta tttaacattt cagtaaatga tgcatttgca atttgatctt taccgatttt
      841 tttcattgta tctgcaactg ttacatctcc agctttagaa gaaattaatg tattttcttt
      901 agagtctgtg gcactagcgc cacaagcgcc taataataaa gcactagctg ttaccggaac
      961 gattaatttg tttatcatct tcatagttga aactcctttg taagttaatc atcgtgaata
     1021 taacatagtt ttatattatc accaatcaat aacccatgac atcagactag agcattaggt
     1081 ttaggggtag aaattgatga aaataaaaaa acaaggtcta tatacatttg attgtaagct
     1141 attacatagc ctaaaaacaa ataataacac cttgctataa ttattttgtt ataaatgata
     1201 tggattttta atttttttca aaatattaat acctgtacta tgttcttcta tatattgtcc
     1261 ataattagaa aatataatta tttttaaata taataaatct gtaatggaaa taccgaaatt
     1321 caatgccaat aagaacataa aataatgtcc atattgcggg aatgaaattg ttagataaac
     1381 gaacattgca gataagataa ttaacggcaa tattaagtta atgcaaaaat aaaatttgtg
     1441 tacaggttga ttgacgtagg tattatagaa tataagccat tttcttttag ttaacttatg
     1501 aactttaaac gattttctat atggtaagaa aaataataaa tgtatacttt tatgtaatgg
     1561 atacgtcata aatacaagta ataaaaagat gagaaaatgt ctatctgata atggcgtatt
     1621 agataaaaaa tacattactt cataactaac taaaaacata atgatggttg caactgcact
     1681 cataaatgca attctaggca aaccaaatcg tgcattgata tcaatttgtc ttttacataa
     1741 aaacatatct tacgcctcat ctcttttcaa tataagttag attataacta actcaggttc
     1801 agtaattcaa tagtaacata tcatgttaga agaattgtta tataagaaga aaggggagac
     1861 acgtattgtt tgatttgaaa cgcgaaagtg gagattggta aaattggctg attgactagc
     1921 gtagaagtag aatgcagaac aaatttctgc gaacacatct tcttgctaat aaagcatcac
     1981 ttatgaaaaa ggcgcgtgtg acatgagtat ttgaaaactt gaataatata aaaaatggat
     2041 atggcagggc atatgcaaaa gcatcttttg accaactaag ttatacaaat gctaaaaata
     2101 tagtgatatg taattataat aaataccaga ttaataattt gtgcaagtaa ggtatatgta
     2161 atgtgtatat aaggttcggc tcaatgaaaa tataagtatg ttgtttgtcg ataatgatat
     2221 aagtaaagcg taatcaaagt gcaaggataa cacaatgaat cctaagcaat gttttcaatc
     2281 aattcataaa tatgatttaa gcgaaaatca ctacaaacga tgtattcctg taatttgtaa
     2341 acataagttc atttgagtta taaaaaagag atgacaagaa atatctaatt tctaatgtca
     2401 tctcgtttat ttggcattta aattaaaaaa ataaatatgc tgttactaat aaaaatagtt
     2461 gtgatcgaga acgtaacgtg ctacttagaa atttcgtttc caatatcctc gccacgattt
     2521 tgtaaatttt cgatgtgtga ctgtaaacgt tcaatattag gattaatgtc agatttgaag
     2581 tttccaagta atgacttaat ttcaccacca attgaactac caaattccgc actttcgttt
     2641 ttaatttgaa caccatagtt taaaatgtca ttaaaacttt gtttaatcgt ttcaaattca
     2701 cgttgtagtt ctgattttga accagtaggg gcagtacgat cgatcgtgtt gttcttgaca
     2761 cttttgtcgt ctcgtccttg aagtgcaact acaaaaccag ctgctacgcc aacaccgata
     2821 ccgaatagaa tgcgtgatgc tttcatttat attctcctca cttttaacat ctgttatctt
     2881 gataactcat tttgtttaaa attgctaacc ttgatgttat cacttcaaac gcaattgtta
     2941 taatattaat catattatac ccgttcgagc ttagattaag catacattta aaattgtgct
     3001 tgaatttgtt cagcaatttg ttgttttgca tcgttatcta aaatgtcttc atgtgtttcc
     3061 cacttataac caaatccatc aatatcattt tcgtatcgag gaattaagtg gaaatgaata
     3121 tgaaatacag attgatctgc aaactcacca ttattttgaa taatgtttaa accatcagga
     3181 ttaaatgcac gcttaatagc atttgctact ttaggtaatg ctgcaccgat atgtttcatt
     3241 gtttcttcat cagtttcaaa gatattagca gaagcttttt taggaattaa taacgtatgt
     3301 cctttagtaa cttgtgatat atctaaaaag gcatagacat aatcgtcttc atatacttta
     3361 aagctaggaa tttctccagt taaaattttg ccgaaaattg tttctgacat gatgactaca
     3421 ctccttttca actagtatta ccattcatta taacattgtc gctaaaaatt cgatattagg
     3481 acatgtgtat ttaatatgag cattttataa tttttggtac aatataagtc atggaggtgc
     3541 cttatgacag ttaaagtaga acagcttaca ggtggatatg gaaaacgccc tgtaattaaa
     3601 gatataaatt ttgaattaaa caaaggcgaa atcgttggac ttattggcct taatggcgct
     3661 ggtaagagta caacgattaa acatatgcta ggattgctta cacctatgga aggatctcta
     3721 tcaatttcag atatcaacat taatgatgac atagaggcat atagaagaaa gttatcttat
     3781 attccggaat caccggttat ttatgaagaa ctcacattag aggaacatat agagatgaca
     3841 gcaatggcat atgatattga tcgtgatgaa gcgatgaatc gagcaatgcc attattaaag
     3901 acattccgtt tagaaaatga attgaaagtt tttccgagtc atttttctaa ggggatgaaa
     3961 caaaaagtca tgattatttg tgcgtttatc gttaatcccg aattgtatat tatagacgaa
     4021 ccattccttg gactagatcc attaggaatt caatccatgt tagatttaat ggttgaaaag
     4081 aaaaacgaag gtagaaccgt tctaatgagt acacatattt tagcaacagc tgaacgttat
     4141 tgtgatcgtt ttatcatact agacgaaggt gaagtcgttg catttggaga tttagaagca
     4201 ttgagacaac aaactggctt acataatcaa acattagacg atatatatat tcatgtgacg
     4261 caaggtggag atgtacatgc ataaccaagc gaccacatta tttaataaaa gattacatgc
     4321 ccttcgtaaa gagaaaaatt attataataa gtttatattt aatggtcatt tcatggtctt
     4381 tttacttatt ttgctgggtg catttatttt tggatatggt gaatggttaa aacatattcc
     4441 aacgaatatc aattttgcat taatagcagc agtgattgtt gcgttgacat ccatttttcc
     4501 aatgcgacca ctcctaaaag aagcggataa aatcttttta ttaccatttg aaaaacacat
     4561 gtctcaattt atgcgccatg cgattttata tagttacttt gcgcgcatat taatccaact
     4621 cattattgtt attgtaatgt tcccgttgtt ttacaatatt aatcaacata atgtggcatt
     4681 ttatatttgt tttggattca gtgcattaat ttttccatat gttggtttac gcttaagatg
     4741 gcaatggtat caatcgggac ttaaaacatg gcaagttaat ttaatatcat ttatcatctt
     4801 tgctctaaca tattacttac tattagctcc aaaatggtac atcgtatttg tgatggttgc
     4861 tttgcctgtg ttaatagaat ttttagttaa aaaatataaa ccaggctttt tgtatccttg
     4921 ggagaaaatg attgctatcg aacatcgaca tcatatgaat tattataaat ttgttaatat
     4981 gttcaccgat gtaaaacatt tgaaagaatc tgcagtcaga agaagttatc tagatatact
     5041 tttaccagtt ccaaagggta gtaaatttaa ctctaatgcg atgtatttat tcttatttat
     5101 tagaagcttt ataagaggga gagacgcatt taatattatc tttaggttag taattattgc
     5161 agttttactt atggtttggt tatcatatcc attagtgaca ggggttattg gaagtctttt
     5221 tgtgtatatt atattgttgc aaatggcaca attttattca caacaagcat atggcttatg
     5281 gcctcaagtg tggcctgtac ctgaagagaa ggtaatcaaa gggtatgaac agtttttata
     5341 tagattaatg tttgttattt gtactgtgtt tgcagtgaca tttattataa aacatatgac
     5401 gttattctat gttgtactca ttttctatat tgtaggttta ttaacgatta gaagtattat
     5461 taaaaagttg aaatatcagg aaacattatt acgagactaa aaaacttcca ttggcatgta
     5521 tgttgtaaag gtgcatgtaa tgttgaacgc caaatgatac ggcgttcaga ttacattagc
     5581 atctatacgt taacagcata accaatggaa gttttcttcg agtctattct tttattgggt
     5641 atagatatct ttcaaaataa cttgaatgtt gtccgcttga accaaagtaa tgacttaatg
     5701 cgtcttttga aaaatggtca ttaaaggcat cagattgctt aaagtcttcg tatgcatgtc
     5761 gatcagcaaa tccgaaataa attttatatg ttgtaccttt agcaggtctt aacaaacgat
     5821 agcttttaaa gccaccaaag tttctgaaat tatcgtctac actaatcagt ttcttttcaa
     5881 gttgatatgc atgatcttct gttgaaggaa tgaagattgc acaatagaaa tgatgttcac
     5941 tgaattcacc aatttcttta ataacttcat atattgaagg tgattttaaa acagtctcac
     6001 catcagtttc ttcaaaaata actgaagtat ctgatgctga aaattggaat agttgatggg
     6061 tcgggttatt gatttttatt tgatgtaaaa atccataagt gccataagat gtatatagtt
     6121 tcttcatatg aaaggtcccc cttatgtttt tatttattat acgatgataa actagtcatt
     6181 accactatta ataattgatt aaaattacta taatgaatct tgcgagatat ttagaagtaa
     6241 tgatacaaaa taatggactt tagaatttaa atttacaata aatagttcta tgattaaatg
     6301 tcagttttat gacatttatt tattgaaaat acgaacgaat gagcgatatg ataatataga
     6361 taagaatgat tttaatttag gaggccttta tggtgcataa taaaaacaat acaattttaa
     6421 aaatgatcaa aggtgaagaa acatcacata cacctgtttg gtttatgcga caagctggcc
     6481 gttcgcaacc agaatatcga aaattgaaag aaaaatattc actattcgat attacacatc
     6541 agccggagtt gtgcgcttat gtaacacatt taccagttga taattatcat acagatgcag
     6601 caattttata caaagatatt atgacaccat taaagccaat tggtgtcgat gtagaaatta
     6661 aatcgggtat tggtccagtg attcataatc ctatcaaaac aattcaagat gttgagaaac
     6721 tttctcaaat agaccccgaa cgagatgtac catatgtatt agatacaatt aaacttttaa
     6781 cagaagaaaa gttaaatgtg ccgctaatag gatttactgg ggcaccattt acattagcgt
     6841 catatatgat tgaaggcgga ccatcgaaaa attacaattt tacaaaagcg atgatgtata
     6901 gagatgaagc aacatggttt gctttaatga atcatttagt tgatgtatct gttaaatatg
     6961 taacagctca agtcgaagca ggtgccgaat tgattcaaat tttcgattca tgggtaggtg
     7021 cattaaatgt cgaggattac agacgttaca ttaaaccaca tatgattcga ttaatcagtg
     7081 aggttaaaga aaaacatgat gtgccggtaa ttttattcgg tgtaggtgcc agtcatttaa
     7141 tcaatgaatg gaatgattta ccgattgatg tattaggcct agattggaga acgtctatta
     7201 atcaggctca acaattaggc gttactaaaa cattacaagg gaatttagat ccatcaattt
     7261 tattagcacc atggaatgtc attgaagaga gattgaaacc aatattagat caaggtatgg
     7321 agaacggtaa acacatcttt aatttaggac acggtgtttt cccagaagtg caaccagaga
     7381 cgttacgtaa agtaagcgaa ttcgtacaca catatacaca aagataatca tagtattaca
     7441 ttgaagcaca tgaaagataa ttcatttaaa ggaaggtatt atcaatgact aaaaaaatgg
     7501 ggttattagt tatggcttat ggcacacctt ataaagaaag tgacatagag ccatattata
     7561 cagatattag acatggtaaa cgtccatctg aagaagaact tcaagatttg aaagatagat
     7621 atgaatttat aggtggttta tcaccattag caggtacaac agatgaccaa gctgatgcac
     7681 tagtttcagc attaaataaa gcatatgcag atgttgaatt taaactatac ttaggattaa
     7741 aacacatttc accatttatc gaagatgcgg ttgaacaaat gcacaatgat ggtattactg
     7801 aagcaatcac ggtagtacta gcaccacatt attcttcatt ttcagtagga tcatatgaca
     7861 aacgtgctga tgaagaagct gcaaaatatg gtattcaact tacacatgtg aaacattatt
     7921 atgaacaacc taaatttatt gaatattgga cgaataaagt caacgaaaca ttagctcaaa
     7981 taccggaaga ggaacataaa gacacggtat tagttgtttc ggcacatagt ttgccaaaag
     8041 gtttaatcga aaagaataat gatccatatc cacaagaact agaacatact gcgcttttaa
     8101 ttaaagaaca atctaatatt gaacatatcg cgattggttg gcaatctgaa ggtaatacag
     8161 gtacaccttg gttagggcca gatgtacaag atttaacacg tgatttatat gaaaaacatc
     8221 agtataaaaa ctttatatat acgccagtag gttttgtatg tgagcattta gaggtgcttt
     8281 atgacaatga ttatgaatgt aaagttgttt gcgatgatat tggtgcgaat tattatcgtc
     8341 caaaaatgcc gaatacacat ccattattta tcggtgcaat tgttgatgaa atcaagtcta
     8401 tattttaatg acgaaagaag cgtgaaacgt tgtgactaaa tcagtggcta ttataggagc
     8461 ggggataaca ggtttatcaa gtgcatattt tttaaaacag caagatccta atattgatgt
     8521 aaccatcttt gaagcatcga atcgtccggg gggaaagatt caatcgtatc gtaaagatgg
     8581 ttatatgatt gaactagggc ctgaatctta tttaggtaga aaaacgatta tgacagaatt
     8641 agcgaaagat attggattag aacaagatat tgttacaaat acgactggac aatcatatat
     8701 ttttgcgaaa aataaattat atccgattcc aggtggttca attatgggta ttccaacaga
     8761 tattaaacca tttgttacta caaaattaat atcgccactt ggtaaattaa gagcaggatt
     8821 agatttaatc aaaaagccta tacaaatgca agatggtgac atttctgttg gtgcattttt
     8881 cagagcaaga ttaggtaatg aggtacttga gaacttaatt gagcctttaa tgggtggtat
     8941 ttatggtacc gatattgata aattaagttt gatgagtacg tttcctaatt ttaaagaaaa
     9001 agaagaggca ttcggaagtc tgataaaagg tatgaaggat gagaaaaata agcgtctgaa
     9061 acaaagacaa ttatatcctg gcgcaccaaa aggacaattc aaacaattta agcatggttt
     9121 aagctcattt attgaagcat tagaacaaga tgtgaaaaat aaaggtgtga caatacgcta
     9181 caatacgtca gtggatgata ttattacatc tcaaaagcaa tataaaattg tttacagtaa
     9241 tcaacaagaa gatgtattcg atggggtatt agtgacaaca ccgcatcaag tctttttgaa
     9301 ttggttcgga caagatccag catttgatta ctttaaaacg atggatagta cgactgttgc
     9361 aactgttgta ttggcatttg atgaaaaaga cattgaaaat acttatgatg gtactggctt
     9421 cgtgattgcg agaacgagtg atacagacat taccgcatgt acttggacat cgaaaaaatg
     9481 gccatttact acaccagaag gtaaggtttt gattcgtgcg tatgtaggta aaccaggtga
     9541 tactgtggtt gatgatcata cagataatga attagtatcg attgtacgta gagatttaag
     9601 tcaaatgatg acatttaaag gtgatcctga atttacaatt gtcaatcgtt tgccgaaaag
     9661 tatgccacag taccatgtcg gtcatattca acaaattaga cagattcaag cacatattaa
     9721 acaaacatat ccacgactta gagtaactgg tgcatctttt gaagcggttg gactacctga
     9781 ttgtattaca caaggtaaag ttgctgctga agaagtaatc gcagagttgt aagttaattg
     9841 ttggatggtg ttggtgtagg tgcgacgcct ttagataatg tttgatgaaa tatagtcatt
     9901 tttaagttat ataatggtgg aagtaatgtg taaatataga tactttcagc gggaattatt
     9961 tttgtgaaat tgaagcagag acaaaggttt agctgttttt gaatgatatt gaaatgatga
    10021 caatacactt taattagaaa tgtcactttg cgatataaca tttctaatta aggtgttttt
    10081 attttacaat tataggtaat cagtcatttt tttaaaaata gaaaagaagt agtaagttga
    10141 aaggagagaa tgagatgaca gatgtaatta ttgtacattc tatgcatgga aatagtagaa
    10201 atcattggta tcagtggttg gagcataact taactttaga aggatatgac gtaacattat
    10261 ttaattttga atcccctgaa gcgaagacag tggatcagtg gattgaagct atgacgaaac
    10321 aaattaatgt acgcaaaaag gatacatatt ttgtaacaca tggtttaggt tcaatcacag
    10381 ctttaaaata tattgaaatg attgatcagc cgattgaagg cttttttagt atagcgggct
    10441 ttaaagaaga tgcagaaaat atagatttag atattgattt aagtaatgta accattgatt
    10501 acgataatat taaaaagaaa gttgacaatt tttacggatt gagttctaaa aatgacaaat
    10561 acgtttcata taaagaaacc cagcgtttaa tgaatgcatt agaggggaac ttgcgaatcg
    10621 tggaagatgg agggcacttc ttagaagagg atggcttcga aacatttaca gcgttgcaag
    10681 atcggatgca agattacatg acacgatagg taatgtttgg ttgctatgta ttgtgttatg
    10741 tgtgtcagta cgaagcatag cactttagtg aaaaagatta taattatatt ttaatagttt
    10801 agaggtgtag gcattataaa atttatgatt cctacatctt tttcaatagt tcatgagtaa
    10861 ttatgacaac tattagacac ttatattata aaagggaaga agaagcgttt ttttacacaa
    10921 aaaactttca aaatgtcgtt gacgtacact acaaaaatga atatactaat atttgtgttc
    10981 aacagagaac atgttgctac gatatttaag taattattta agagataaca tttaaattaa
    11041 attcaaaaag ttcttgactt taggtttttg attatggtat attaattagg taattcaaaa
    11101 taatatta
//
LOCUS       DAOBBH010000080        10987 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.080, whole genome shotgun sequence.
ACCESSION   DAOBBH010000080 DAOBBH010000000
VERSION     DAOBBH010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10987
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.080"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            51..812
                     /gene="sufC"
                     /locus_tag="P6L01_001483"
     CDS             51..812
                     /gene="sufC"
                     /locus_tag="P6L01_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDP3030554.1"
                     /translation="MASTLEIKDLHVSIEDKEILKGVNLTINTDEIHAIMGPNGTGKS
                     TLSSAIMGHPSYEVTKGEVLLDGVNILELEVDERAKAGLFLAMQYPSEITGVTNADFM
                     RSAINAKREEGQEINLMQFIKKLDKNMDFLDIDKDMAQRYLNEGFSGGEKKRNEILQL
                     MMLEPKFAILDEIDSGLDIDALKVVSKGINQMRGENFGALMITHYQRLLNYITPDKVH
                     VMYAGKVVKSGGPELAKRLEEEGYEWVKEEFGSAE"
     gene            910..2217
                     /gene="sufD"
                     /locus_tag="P6L01_001484"
     CDS             910..2217
                     /gene="sufD"
                     /locus_tag="P6L01_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDP3030555.1"
                     /translation="MTTDILNISEEQLVDYSKAHNEPSWMTELRKKALKLTETLEMPK
                     PDKTKLRKWDFDSFKQHDVKGDVYQSLSQLPESVREIIDVDHSKNLVIQHNNTIAYTQ
                     VDDNASKDGVIVEGLADALMNHSDLVQKHFMKDAVTVDEHRITALHTALVNGGVFVYV
                     PKNVVVEHPVQYVVLHDDKNASFYNHVIIVTEESAEVTYVENYLSNASGEGNQLNIIS
                     EVIAGANSNITYGSVDYMDKGFTGHIIRRGITEADASINWALGLMNEGSQIIDNTTNL
                     FGDRSTSSLKSVVVGTGEQKINLTSKIVQYGKETDGYILKHGVMKEHASSVFNGIGYI
                     KHGGTKSIANQESRVLMLSEHARGDANPILLIDEDDVQAGHAASVGRVDPDQLYYLMS
                     RGISQREAERLVIHGFLDPVVRELPIEDVKRQLREVIERKVSK"
     gene            2323..3573
                     /locus_tag="P6L01_001485"
     CDS             2323..3573
                     /locus_tag="P6L01_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDP3030556.1"
                     /translation="MNEVAEHSFDVNEVIKDFPILDQKVNGKRLAYLDSTATSQTPMQ
                     VLNVLEDYYKRYNSNVHRGVHTLGSLATDGYENARETVRRFINAKYFEEIIFTRGTTA
                     SINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKNATLKFIPMTADGELNI
                     EDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIAHQHGAIISVDGAQAAPHMKLDM
                     QEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIEFGGDMIDFVSKYDATWADLP
                     TKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTIYAYEQMSAIEGIEIYGPP
                     KDRRAGVITFNLQDVHPHDVATAVDTEGVAVRAGHHCAQPLMKWLNVSSTARASFYIY
                     NTKEDIDQLINALKQTKEFFSYEF"
     gene            3563..4027
                     /locus_tag="P6L01_001486"
     CDS             3563..4027
                     /locus_tag="P6L01_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDP3030557.1"
                     /translation="MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDR
                     IRLTFDIEDGIIKDAKFEGEGCSISMASASMMTQAVKGHSLGEAMQMSQEFTKMMLGE
                     DYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE"
     gene            4178..5575
                     /gene="sufB"
                     /locus_tag="P6L01_001487"
     CDS             4178..5575
                     /gene="sufB"
                     /locus_tag="P6L01_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDP3030558.1"
                     /translation="MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENIVREISNMKNEP
                     EWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRT
                     FDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKY
                     FASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLII
                     ADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRT
                     FVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHK
                     APNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYN
                     EVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAV
                     EMNRLIKFEMEGSIG"
     gene            complement(5643..6692)
                     /locus_tag="P6L01_001488"
     CDS             complement(5643..6692)
                     /locus_tag="P6L01_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDP3030559.1"
                     /translation="MPVYKDDNTGKWYFSIRYKDVYGNNKRKMQRGFSTKREAKRAEA
                     IFLNDVNEGYSDSKTFDYVFHHYLENSDLRPKTKRRKQNEYHKHFKAKFGHIKMNKIT
                     QNQCQEFRKYLIENVASTNSARTIWSGFKVVINYAKKYFGLRTDPTISIKPIPRVKPK
                     PKFMMREEFEERIKDIEDQDYRELFTLMFYTGLRIGEAMALVWTDYNKYKKEISINKT
                     MDISNRTIYPRPKTDSSEDIVPLPKFINTMLTERHQREKELNKYFDERSYFIFGGMAP
                     KHYSHVQKKFQKAFPHYNIHALRHSYASYLANNGVDIFVLQSLMRHAQITETMGTYSH
                     LYTQKKHDAIAIFDK"
     gene            6804..7004
                     /locus_tag="P6L01_001489"
     CDS             6804..7004
                     /locus_tag="P6L01_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDP3030560.1"
                     /translation="MTFKNNHNFNELVLTNEDIRILKNVLEDAVSVYDEYSVCNEESD
                     FAYCLLRDLYTLDSLAISSNNV"
     gene            complement(6941..7846)
                     /locus_tag="P6L01_001490"
     CDS             complement(6941..7846)
                     /locus_tag="P6L01_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030561.1"
                     /translation="MEEKQYLWRYNDIEKRMNELHKKYKELVDIFFGDVIDKNTGYFP
                     FYSGIKYSYADLNISFYRGLIYIHGGSEGMQAKKINISVDDVLKRTERSFEKLSEYIK
                     STFIFEKVIHDFQMFNLSDLASIYNDDAIAHYYFETHSDLYLSDKSTKIYRTPNSLLE
                     NANDLPASLRKYTQLLETVNDKDFETHIVEAYDCFMSEKRLATSLLLGRALELMCRLI
                     LNKFDKDIIKQTPDYKRNIRTFLNEMENNDLIEEHLKHSVKAAIEHRNSIMHGIKIEE
                     YNSIIQTLFDEIAKLSNVYKSLNKQ"
     gene            complement(7878..8603)
                     /locus_tag="P6L01_001491"
     CDS             complement(7878..8603)
                     /locus_tag="P6L01_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015977653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDP3030562.1"
                     /translation="MKELPKSRLTFKESMIESQYLATKTKEEKKQYKQLSVEDKREIL
                     KEYQSKPRKEVKFESEINKSDENLSKIYQRFSEIGVEDLFGTKKEVKELPMILKDNEN
                     IMYVTSGLYNNNTYLIVCTDLRLLFLDKGMIYGLKFHEFPFEKINSVSYKKGLLFGEI
                     IIHHGSSSIAIGSISKNTVSRMAETIQEQISIRESSMKPSNSEKMSFSVADELIKYKE
                     LLDVGVISQEEFDKKKQQLLDID"
     gene            complement(8631..9305)
                     /locus_tag="P6L01_001492"
     CDS             complement(8631..9305)
                     /locus_tag="P6L01_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDP3030563.1"
                     /translation="MKLNYEKSFFKSAKAVYEITNGLYNLSFPLDIFEIISKDKRIKL
                     VTFSEFSQNTGTLYFKIPSIFGSEEAFHIRKGDKAIIVYNDLLPMNRLRFTLAHEYGH
                     FIMGHTGVNLNKTFTYKDYYRRIAEEYEANSFASCLLFPLHIRYKYINNFNIEQISYK
                     YQMSFQAIHIAVKVIRRHIHNGLNDYMSNNENYHAENYLSFLEEKMESKSDFINEFKY
                     AYDLTI"
     gene            complement(9322..9654)
                     /locus_tag="P6L01_001493"
     CDS             complement(9322..9654)
                     /locus_tag="P6L01_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDP3030564.1"
                     /translation="MNLVQRIRNLCNSKGMTFAELERTLGFSNGQIRRWEKTKPGIDK
                     VQKIADHFDVSVDYLLGREKDEYSGEDKSEDILIMHRATENMTEAQRQKALTILEAMF
                     DDWDDLTK"
     gene            9917..10111
                     /locus_tag="P6L01_001494"
     CDS             9917..10111
                     /locus_tag="P6L01_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDP3030565.1"
                     /translation="MSTTDFGLKVRTELLKRNMTNKQLAEMLEISSAYLSDILRGRRD
                     AFEQKKRIAKILEIKEEVKS"
     gene            10111..10875
                     /locus_tag="P6L01_001495"
     CDS             10111..10875
                     /locus_tag="P6L01_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HDP3030566.1"
                     /translation="MNEIKTFSNDMFSILIKQDNENNLFDLETVAKSLGFTQFKNGKQ
                     YIRWETINKYLGKYLSQEVGKGDFIPEPMVYKLAFKAGNAVAEKFQDWLAMEVLPAIR
                     KHGIYATDNVIEQTLKDPDYIITVLTEYKKEKEQNLLLQQEIGELKPKADYVDEILKS
                     TGTLATTQIAADYGISAQKLNKLLHEARLQRKVNKQWVLYSEHMGKSYTESDTIAIVR
                     SDGREDTVLQTRWTQKGRLKIHEIMTEFGYEANVTA"
BASE COUNT         3754 a         1679 c         1908 g         3646 t
ORIGIN      
        1 ttattataaa ctagttaaga aaactttata ttttacggag ggaatataaa atggcatcaa
       61 cattagaaat caaagaccta catgtgtcta ttgaggataa agaaatctta aaaggtgtta
      121 acttgacaat taacactgat gaaatacatg cgattatggg accaaacggg acaggtaaat
      181 caactttatc atctgcaatt atgggacacc caagctatga agtaactaaa ggagaagtac
      241 ttttagacgg tgtaaatatt ttagaattag aagttgatga aagagcaaaa gcaggattat
      301 tcttggcaat gcaatatcca tcagaaatta caggtgttac aaatgctgat ttcatgcgtt
      361 cagcaatcaa tgcgaaacgt gaagaaggac aagaaatcaa cttaatgcaa tttattaaga
      421 aattagataa aaacatggat tttctagaca tagataaaga catggcacaa cgttatttaa
      481 atgaaggttt ctcaggtgga gagaagaaac gtaacgaaat cttacaatta atgatgttag
      541 aacctaagtt tgcaatctta gatgaaatcg attcagggtt agacatcgat gcattaaaag
      601 ttgtatctaa aggtattaac caaatgcgtg gggaaaactt tggtgcatta atgattacac
      661 actatcaacg attattaaat tacattactc ctgataaagt acatgtaatg tatgctggta
      721 aagtcgttaa atctggtggt ccagaattag caaaacgtct tgaagaagaa ggatatgaat
      781 gggttaaaga agagttcggt tcagctgaat aatcttatta atacagtatc catgagatgt
      841 tcatctatat atgatgaaaa tgaacattta tacgaaatag taaatttcat caagtaggag
      901 gaaaaagtta tgacaactga tattttgaac atttctgaag aacaacttgt tgattattct
      961 aaagcccaca atgaaccttc ttggatgaca gaattacgta aaaaagcttt gaaattaaca
     1021 gaaactttag aaatgccaaa acctgataaa acaaaattaa gaaaatggga ttttgattct
     1081 tttaaacaac acgatgtaaa aggtgatgtt tatcaatctt tatcacaatt acctgagtca
     1141 gtaagagaaa ttattgacgt agatcattct aaaaacttag taattcaaca taataatacg
     1201 attgcgtaca cacaagttga tgataatgca tcgaaagatg gcgttatcgt tgaaggttta
     1261 gcagacgctc ttatgaacca tagtgattta gtacaaaagc actttatgaa agatgcagta
     1321 acagtagatg aacatcgtat cacagcgcta cacacggcat tagttaatgg tggcgtattt
     1381 gtttatgttc ctaaaaatgt agttgtagaa catccagtac aatacgttgt gttgcacgac
     1441 gacaaaaatg caagctttta taaccatgtt atcatcgtta ctgaagaaag cgccgaagtc
     1501 acatatgttg aaaattactt atcaaatgca tctggtgaag gaaatcaatt aaatattatt
     1561 tctgaagtga ttgctggtgc aaattcaaat atcacatatg gctcagtgga ctatatggat
     1621 aaaggcttta caggtcatat cattcgacgt ggtattactg aagcggatgc ctcaattaat
     1681 tgggcactag gtttaatgaa tgagggtagc caaattattg ataatacaac aaatttattt
     1741 ggtgatcgtt caacaagttc acttaaatca gtagttgtag gtacaggaga acaaaaaatt
     1801 aatctaacat ctaaaatcgt acaatatggt aaagaaacag atggttatat ccttaaacat
     1861 ggtgttatga aagaacatgc atcatctgta tttaatggta tcggctacat taagcatggt
     1921 ggaactaaat caattgctaa tcaggaatca cgtgtattaa tgttatctga acatgctcgt
     1981 ggtgacgcga atcctatttt attaattgat gaagatgatg tacaagctgg tcatgctgca
     2041 tcagtaggtc gtgttgatcc agatcaactt tactatttaa tgagtcgtgg tatttctcaa
     2101 agagaagcgg aacgtcttgt tatacatggt ttcttagatc cagtagtacg tgaattacct
     2161 atcgaagacg ttaaacgtca attgagagaa gtaattgaac gcaaagtttc taaataatat
     2221 tttgaaaata aaagtttgta atagatatag actgtcgata ttggtataag actaatacaa
     2281 cgtcagtatt taaatgatta ggatttttat ttaagaaagg tcgtgaatga agtggccgaa
     2341 cactcatttg acgttaatga agtaatcaag gattttccga tattagatca aaaagtcaat
     2401 ggcaaacgtt tagcatatct tgattcaaca gcgacaagtc aaacgcctat gcaagtgtta
     2461 aatgttttag aggattacta caagcgttat aattcaaacg ttcatcgtgg tgttcataca
     2521 ttaggatcat tggcaactga tggttatgaa aatgcccgtg aaaccgttcg tcgttttatt
     2581 aatgcgaagt attttgaaga aatcattttc acacgcggaa caactgcgtc gattaacctt
     2641 gtagcacata gctatggtga tgcaaatgtt gaagagggcg atgaaattgt tgtcactgaa
     2701 atggagcatc atgccaatat tgttccttgg caacagttag caaagcgtaa aaatgcgaca
     2761 ttgaaattta taccaatgac agctgacggt gaattaaaca tcgaagatat taagcaaacg
     2821 attaatgata aaacaaagat cgttgctatt gcacatattt ctaatgtact cggtacaatt
     2881 aatgatgtta aaaccattgc agaaatagct catcaacatg gcgcaattat cagtgttgat
     2941 ggggcgcaag cagcaccaca tatgaaactt gatatgcaag aaatgaatgc tgatttttat
     3001 agttttagtg gtcataaaat gcttggacca acaggtattg gcgtattatt tggtaaacgt
     3061 gagttactac aaaaaatgga accgattgag ttcggtggtg acatgattga ttttgtaagt
     3121 aagtatgatg caacatgggc tgatttacct actaaatttg aggcgggtac tccattaatt
     3181 gctcaagcaa ttgggcttgc agaagctatt cgctatttag aacgcatagg ttttgatgca
     3241 attcataaat atgaacaaga attaacgata tatgcttatg agcaaatgtc tgcaattgaa
     3301 ggaattgaaa tttatggccc gcctaaggat cgtcgtgcag gtgtaataac gtttaattta
     3361 caagatgtac atccacacga tgttgctaca gccgtagata cagaaggtgt agcggttaga
     3421 gctgggcatc attgtgcgca accgttaatg aaatggttaa atgtgtcttc aacagctaga
     3481 gcgagttttt atatatacaa cacgaaagaa gacattgatc agttaataaa tgccttgaaa
     3541 caaacgaagg agtttttctc ttatgaattt taataatcta gatcaattat atagatctgt
     3601 cattatggat cattataaaa atcctagaaa taaaggtgta ttagataacg ggtctatgac
     3661 agtagatatg aataacccga catgcggtga ccgtatacga ctaacatttg atatagaaga
     3721 cggcattata aaagatgcta agtttgaagg tgaaggttgt tcgatttcaa tggcaagtgc
     3781 atcgatgatg acacaagctg ttaaaggtca ttcacttgga gaagcaatgc aaatgagcca
     3841 agaatttacg aaaatgatgc ttggtgaaga ctatgtgatt acagaagaaa tgggagatat
     3901 tgaagcattg caaggtgtat ctcaattccc agctcgtatt aaatgtgcca cattagcttg
     3961 gaaagcattg gaaaaaggta ctgttgctaa agaaggtaaa gcagaaggta cgactgaaga
     4021 agaatagaat gctgttaatc atagatgatt ttgatattag acatataaaa gtataaaaat
     4081 ttttataaga tgtcatgtca ttgttataat atggtttaca tcatgaatta aaaacttacg
     4141 cacgccgttg taaatatatt tttaaggagt gattgaaatg gctaaaaaag cacctgatgt
     4201 tggggattat aaatatggat tccacgacga tgatgtatcc attttcagat cagaacgtgg
     4261 tttaactgag aatatcgtta gagaaatttc taacatgaaa aatgagccgg aatggatgtt
     4321 agatttccgt cttaaatcat taaaattgtt ttataaaatg ccaatgcctc aatggggtgg
     4381 cgacttatca gaattgaatt tcgatgacat tacttactat gtaaagcctt cagaacaagc
     4441 tgaacgttca tgggatgaag tgccagaaga aattaaaaga actttcgata aattaggaat
     4501 tcctgaagct gaacaaaaat atttagctgg tgtttctgct caatatgaat ctgaagttgt
     4561 ttaccataat atggaaaaag aacttgaaga aaaaggtatt atctttaaag atacagatag
     4621 tgctttacaa gaaaatgaag aattattcaa aaaatacttt gcttctgtag tacctgcagc
     4681 agataacaaa tttgcggcgt taaactcagc agtatggtca ggtggttcgt tcatttatgt
     4741 acctaaaaat atcaaactag atacgccact acaagcttat ttccgtatta actctgagaa
     4801 catgggtcaa tttgaacgta cattaatcat tgctgatgaa ggtgcttctg tacattacgt
     4861 agaaggttgt actgcaccag tttatacaac tagttcttta cactctgctg ttgtggaaat
     4921 cattgtgcat aaagatgcgc acgttcgtta tactacgatt caaaactggg cgaacaatgt
     4981 atacaattta gttacaaaac gtacttttgt ttatgaaaac ggaaatatgg aatgggtaga
     5041 tggtaactta ggttctaagt taacgatgaa atatccaaac tgtgttcttt taggtgaagg
     5101 tgcaaaaggt agtacattat ctattgcatt tgctggtaaa ggacaagttc aagatgccgg
     5161 tgctaaaatg attcataaag cgccaaatac atcttcaacg attgtttcta aatctatttc
     5221 taaaaatggt ggtaaagtta tttatcgcgg tattgttcat tttggacgta aagcaaaagg
     5281 tgctcgttca aatattgaat gtgatacatt aatcttagat aacgaatcaa catcagatac
     5341 aattccatat aacgaagtat tcaacgatca aatatcatta gaacatgaag ccaaggtttc
     5401 aaaagtttct gaagaacaat tattttatct aatgagtcgt ggtatttctg aagaagaagc
     5461 gacagaaatg attgttatgg gattcatcga accatttaca aaagaacttc caatggaata
     5521 cgcggtcgaa atgaaccgtt taatcaagtt cgaaatggaa ggtagtattg gatagcttta
     5581 aaccgcgttg ttaagccatt cttaacttcc gaaaatggct attgatacca ttttgatacc
     5641 atttacttgt caaaaatggc tattgcatcg tgttttttct gagtatataa atggctgtaa
     5701 gtgcccatcg tttcagtgat ttgagcatgt ctcataagtg actgtaaaac gaaaatatct
     5761 acaccattat ttgcaagata agatgcataa gaatgtctta acgcgtgaat gttataatgg
     5821 gggaaagctt tttggaattt cttttgaaca tgactgtaat gtttgggagc cattcctccg
     5881 aaaataaaat aactacgttc atcaaaatat ttgtttaact ctttttcacg ttggtgtcgt
     5941 tcagttaaca ttgtattgat gaatttaggt aaaggaacaa tatcctctga actatctgtt
     6001 tttggtctcg gatatatagt tctattagag atgtccattg ttttatttat ggatatctct
     6061 tttttatatt tattgtagtc tgtccaaaca agcgccatag cttcgccaat ccttaaacct
     6121 gtataaaaca ttaatgtaaa taactctctg taatcttgat cttcaatgtc tttgattctt
     6181 tcttcaaatt cttcacgcat cataaactta ggttttggct ttacacgcgg aataggttta
     6241 attgatattg ttggatctgt acgtaatcca aagtattttt tagcataatt aattacaact
     6301 ttaaaacctg accaaattgt acgagcagaa tttgttgatg ctacattctc tattagatat
     6361 ttacgaaact cttggcattg attttgtgtt atcttattca tttttatgtg cccgaactta
     6421 gctttaaagt gtttatgata ttcattttgt ttgcgtcgtt ttgttttagg tctcaaatcg
     6481 ctattttcta agtagtgatg aaaaacataa tcaaatgttt tcgaatcact atatccttcg
     6541 tttacgtcat tcaaaaaaat agcctctgct ctcttagctt cacgcttagt tgaaaaaccg
     6601 cgttgcatct tacgtttgtt attaccgtat acatctttat atctaatgga aaaataccat
     6661 ttacctgtat tatcatcctt atatactggc attttgcttc tccctcctca aaattggcaa
     6721 aaaataataa gggtaggcgg gctacccgaa atttagtact aggtactaaa tgtgatataa
     6781 taaaataaaa agtaggtgat gttatgacat ttaaaaacaa tcataatttc aatgaattag
     6841 ttttaacgaa tgaagacatt agaattttaa aaaatgtctt agaagatgca gtcagtgttt
     6901 atgatgaata ttcggtatgt aatgaagaat ccgattttgc ttactgttta ttaagagact
     6961 tatatacatt agacagctta gctatttcgt caaataatgt ttgaattatc gaattgtact
     7021 cttcgatttt aataccatgc ataatagagt ttctgtgttc aatagcagct ttgactgaat
     7081 gttttaaatg ttcttctatt aaatcgttgt tttccatttc gtttaaaaat gttcttatat
     7141 tcctcttgta atcaggtgtt tgtttaatta tatctttatc aaacttgttc aatatcagcc
     7201 tacacattag ttctagcgct ctacctaaaa gtagtgatgt agctagcctt ttttcagaca
     7261 taaagcaatc ataagcttca actatatgag tttcaaaatc cttgtcatta actgtttcta
     7321 ataattgagt gtattttctt aaagaagccg gtaaatcatt tgcgttttct aacaaagaat
     7381 taggtgttcg ataaattttt gtagatttat ctgataaata taagtcagaa tgagtttcaa
     7441 aataataatg cgcaattgca tcgtcgttgt atatactagc taaatcgctc aagttaaaca
     7501 tttgaaaatc atgtatgact ttttcgaaaa tgaaagtact ttttatatat tcacttaact
     7561 tctcgaaaga tctttctgtt ctttttaaaa catcatctac agaaatattt attttcttcg
     7621 cttgcatgcc ttctgaccca ccatgaatat aaattaaacc tctgtaaaaa ctgatattca
     7681 aatctgcgta gctatatttt ataccggaat aaaaggggaa gtatccagta tttttatcta
     7741 ttacatcacc aaaaaatata tccactaatt ctttatattt tttgtgaagt tcattcattc
     7801 ttttttctat atcgttatat ctccataagt attgtttctc ttccatccct catcctcctc
     7861 acgccacaca agcgctatta atcaatatcc aataattgtt gttttttctt atcgaactct
     7921 tcctgagaaa ttactccgac atctaataat tctttatatt ttattaattc atcagcaaca
     7981 gaaaaactca ttttttcaga attggatggt ttcatagaac tttctcgaat agagatttgt
     8041 tcttgtattg tttccgccat tctagataca gtgttttttg atatgcttcc tatagcgata
     8101 cttgatgaac cgtgatgtat aattatttcg ccaaaaagaa gtcctttttt atacgaaaca
     8161 gaattgattt tctcgaatgg aaattcatga aatttcaaac catatatcat acctttatct
     8221 aagaataaca atcttagatc agtacatact attaagtagg tattattatt gtacaatccc
     8281 gaagttacat acattatgtt ttcattatct tttaaaatca taggtagttc tttcacttct
     8341 ttttttgtac caaacaaatc ctctacacct atttcgctaa atctttggta gattttagat
     8401 aagttttcgt cagatttatt gatttcactt tcaaatttca cttcttttct aggtttactt
     8461 tggtattctt ttaaaatttc tcttttgtct tcaacagata gttgcttgta ttgtttcttt
     8521 tcttcttttg ttttagttgc taaatattga ctctcaatca tactttcttt gaacgttaat
     8581 ctgctcttag gtaattcttt catgttcatt tctcctttat tttttgattg ttaaatcgtt
     8641 agatcataag catatttaaa ttcatttata aaatcagatt tgctttccat tttctcttct
     8701 aaaaaactta agtagttttc tgcgtggtaa ttttcgttat ttgacatata gtcgtttaac
     8761 ccattgtgta tatgtcttct gattactttt accgctatat ggatcgcttg aaaactcatt
     8821 tgatacttgt acgaaatttg ctcaatatta aagttgttta tatatttgta tcttatatgt
     8881 aaaggaaaca ataaacatga agcaaatgag tttgcttcat attcttcagc aatccttcta
     8941 taataatctt tatatgtgaa tgttttattt aaattaactc cagtatgtcc cattataaaa
     9001 tgaccatatt catgagctaa agtaaatctt agacgattca taggcagtaa atcgttataa
     9061 actataatcg ctttgtctcc ttttctaata tgaaacgctt cttctgaacc gaaaatagaa
     9121 ggtattttaa aatataaagt gccagtattc tgagaaaatt cagagaaagt cactaattta
     9181 atacgtttat cttttgagat aatttcaaat atatctaaag gaaaagataa gttatataga
     9241 ccatttgtga tctcgtaaac tgctttcgca gatttaaaaa aagatttttc ataatttaat
     9301 ttcaattaaa aagccccttt gttacttagt taaatcatcc caatcatcaa acattgcttc
     9361 taatatagtc aaagcttttt gcctttgtgc ctccgtcata ttttctgtag ctcgatgcat
     9421 aataagaata tcttcacttt tatcttctcc ggagtactca tctttttctc tacctaataa
     9481 gtaatcaact gatacatcga agtgatcggc aattttttgc accttatcaa tgcctggttt
     9541 ggttttctcc catcttctga tttgtccgtt tgaaaaccct aaagttctct ctaattcagc
     9601 aaaagtcata ccttttgaat tgcacaaatt acggattctt tgtactagat tcataaattt
     9661 ctcctatcac agattaactt tttcgctatt tttgttgaca attagcataa aagttaatat
     9721 actgtattta agctttaaat ttagcttact aaacacataa caattattcg ttggggaacg
     9781 agtattcaat acctttatga caggcattac gaattgttat aggtttatta aactatgctt
     9841 aaatattagc ataaaagtta tcggtgttca acagataatt tatttgctta gaaaaaatgt
     9901 tataggaggt gctaatatgt cgacaacaga tttcggcttg aaagtgagaa cggaattatt
     9961 aaaacgcaac atgacaaaca agcaacttgc ggaaatgcta gaaatttcaa gtgcttactt
    10021 atcggatatt ttacgtggac gtagagatgc ttttgaacaa aagaaacgta ttgcgaaaat
    10081 tttagaaatt aaagaagagg tgaagagtta atgaatgaaa ttaaaacttt cagtaacgac
    10141 atgttttcaa tcttaatcaa acaagataat gaaaataatt tattcgattt agaaactgtc
    10201 gcaaaaagtt tggggttcac tcagtttaaa aacggcaaac aatatattcg ttgggaaact
    10261 atcaataaat atttaggtaa atatctttcc caagaagttg ggaaaggcga tttcatacca
    10321 gaaccaatgg tatataagtt ggctttcaaa gcaggtaatg ctgtagcaga aaaatttcaa
    10381 gattggttgg cgatggaagt cctaccagct attcgcaaac acggtatcta cgcaacagac
    10441 aatgtaattg aacaaacatt aaaagatcca gactacatca ttacagtgtt gactgagtat
    10501 aagaaagaaa aagagcaaaa cttactttta caacaagaaa tcggagagct aaaacccaaa
    10561 gcagattatg ttgatgaaat cttaaaatca actggcacat tagctacaac tcaaatcgcg
    10621 gcagactacg gtatatcagc acaaaagtta aacaaactac tacacgaagc tagattacaa
    10681 cgaaaagtaa ataaacagtg ggtactttac tcagaacaca tgggcaagag ttacacagaa
    10741 tcagacacta tagcaattgt acgctctgac ggtagagaag acacagtttt acaaactaga
    10801 tggacacaaa aaggcagatt gaaaatacat gaaatcatga ctgaattcgg ttatgaagct
    10861 aacgtaactg cttaacagga gggcgcagca aatgcaagct caaaacaaaa aagtcattta
    10921 ttactactat gacgaagccg gtaatagacg actattatca attggtaatt tagataccta
    10981 tttatta
//
LOCUS       DAOBBH010000081        10827 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.081, whole genome shotgun sequence.
ACCESSION   DAOBBH010000081 DAOBBH010000000
VERSION     DAOBBH010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10827
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.081"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            24..1712
                     /gene="ilvD"
                     /locus_tag="P6L01_001496"
     CDS             24..1712
                     /gene="ilvD"
                     /locus_tag="P6L01_001496"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDP3030567.1"
                     /translation="MRSDMIKKGDHQAPARSLLHATGALKSPTDMNKPFVAICNSYID
                     IVPGHVHLRELADIAKEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADA
                     AETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTL
                     SSMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLALPYNG
                     TALAVSDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLH
                     TLAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKD
                     GTLHPDRITVTGKTLRENNEGKEIKNFDVIHPLDAPYDAQGGLSILFGNIAPKGAVIK
                     VGGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAP
                     TSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTN
                     RTLNVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI"
     gene            1740..3509
                     /gene="ilvB"
                     /locus_tag="P6L01_001497"
     CDS             1740..3509
                     /gene="ilvB"
                     /locus_tag="P6L01_001497"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HDP3030568.1"
                     /translation="MSKTQHEVNQNIDPLKMAESLELEQLNEKTLNDMRSGSEVLVEA
                     LLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVV
                     VTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
                     QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTE
                     PENKDIDTFISLLKEAKKPVVLAGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVP
                     YEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDAFAPNAKIVHVDID
                     PSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVKHCQNNKQKHPFKLGEEDQ
                     VFCKPQQTIEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGI
                     PSSIGAKLANPDKTVVCFVGDGGFQMTNQEMALLPEYGLDVKIVLINNGTLGMVKQWQ
                     DKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDAAFAYQGPVLIEVR
                     ISPTEAVTPMVPSGKSNHEMEGL"
     gene            3509..3763
                     /locus_tag="P6L01_001498"
     CDS             3509..3763
                     /locus_tag="P6L01_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDP3030569.1"
                     /translation="MTRILKLQVADQVSTLNRITSAFVRLQYNIDTLHVTHSEQPGIS
                     NMEIQVDIQDDTSLHILIKKLKQQINVLTVECYDLVDNEA"
     gene            3885..4904
                     /gene="ilvC"
                     /locus_tag="P6L01_001499"
     CDS             3885..4904
                     /gene="ilvC"
                     /locus_tag="P6L01_001499"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDP3030570.1"
                     /translation="MEEFIMTTVYYDQDVKTDALQSKKIAVVGYGSQGHAHAQNLKDN
                     GYDVVIGIRPGRSFDKAKEDGFDVFPVAEAVKQADVIMVLLPDEIQGDVYKNEIEPNL
                     EKHNALAFAHGFNIHFGVIQPPADVDVFLVAPKGPGHLVRRTFVEGSAVPSLFGIQQD
                     ASGQARNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETL
                     VEAGYQPELAYFEVLHEMKLIVDLMYEGGMENVRYSISNTAEFGDYVSGPRVITPDVK
                     ENMKAVLTDIQNGNFSNRFIEDNKNGFKEFYKLREEQHGHQIEKVGRELREMMPFIKS
                     KSIEK"
     gene            4934..6463
                     /locus_tag="P6L01_001500"
     CDS             4934..6463
                     /locus_tag="P6L01_001500"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDP3030571.1"
                     /translation="MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIE
                     AGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIAT
                     SPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
                     AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAI
                     EGGARRIEGTVNGIGERAGNAALEEVALALYVRNDHYGAQTALNLEETKKTSDLISRY
                     AGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGK
                     HAFSEKLKALGYNIDKEAQIDLFKQFKTIADKKKSVSDRDIHAIIQGSEHEHQALYKL
                     ETLQLQYVSSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYR
                     INSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVG
                     N"
     gene            6466..7512
                     /gene="leuB"
                     /locus_tag="P6L01_001501"
     CDS             6466..7512
                     /gene="leuB"
                     /locus_tag="P6L01_001501"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDP3030572.1"
                     /translation="MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFG
                     GASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFAN
                     IRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
                     EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDA
                     CSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGS
                     APDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYNMIEHGQTTADLGGKLN
                     TTDIFEILSQKLNH"
     gene            7526..8896
                     /gene="leuC"
                     /locus_tag="P6L01_001502"
     CDS             7526..8896
                     /gene="leuC"
                     /locus_tag="P6L01_001502"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDP3030573.1"
                     /translation="MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGL
                     RLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSD
                     EQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQT
                     KPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTI
                     CNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELD
                     VSTLEPQVTWGTNPEMGVNFSEPFPEINDINDQRAYDYMGLEPGQKAEDIDLGYVFLG
                     SCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWR
                     EPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVD
                     VRKVVV"
     gene            8897..9469
                     /gene="leuD"
                     /locus_tag="P6L01_001503"
     CDS             8897..9469
                     /gene="leuD"
                     /locus_tag="P6L01_001503"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDP3030574.1"
                     /translation="MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPF
                     AFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIII
                     AGSFSDIFYMNCTKNAMLPIVLEKSAREHLAQYEEIEIDLPNQTVSSPDKRFHFEIDE
                     TWKNKLVNGLDDIAITLQYESLIEKYEKSL"
     gene            9484..10752
                     /gene="ilvA"
                     /locus_tag="P6L01_001504"
     CDS             9484..10752
                     /gene="ilvA"
                     /locus_tag="P6L01_001504"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HDP3030575.1"
                     /translation="MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYL
                     KREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIF
                     MPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTI
                     SGQGTLAKEMLEQSKTDNVNFDYLFAAIGGGGLISGISTYFKTYSPTTKIIGVEPSGA
                     SSMYESVVVNNQVVTLPNIDKFVDGASVARVGDITFEIAKENVDDYVQVDEGAVCSTI
                     LDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCVISGGNNDINRMKEIEERSLLY
                     EEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHD
                     DLIQLKQRVNHFDPSNIYINENKMLYSLLI"
BASE COUNT         3669 a         1787 c         2109 g         3262 t
ORIGIN      
        1 taaaatttac gggggaattt actatgcgaa gcgacatgat caaaaaagga gatcaccaag
       61 caccagcaag aagtctttta catgccacgg gcgcgctaaa aagtccaact gatatgaaca
      121 aaccatttgt agctatttgt aactcttata ttgatattgt tcctggacat gttcacttga
      181 gagagcttgc agatatagct aaagaagcaa ttagagaagc cggtgccatt ccatttgaat
      241 tcaatacaat tggtgttgat gatggaatag ctatgggaca tatcggaatg cgatattctc
      301 taccatcacg tgaaattatt gcagatgcag ctgaaactgt aattaacgct cattggtttg
      361 acggcgtatt ttacattcct aattgtgaca agattacacc cggtatgatt ttagcagcca
      421 tgaggacaaa cgtaccagct atcttttgct ctggtggacc aatgaaagct ggcttatctg
      481 cacatggaaa agcattaaca ctttcatcaa tgtttgaagc agtcggcgca tttaaagaag
      541 gatcgatttc taaagaagaa tttttagata tggaacaaaa tgcctgccct acttgtggtt
      601 catgtgctgg gatgtttact gcaaattcaa tgaactgttt gatggaagtt ttaggtctag
      661 cattaccata caacggtact gcacttgcag tcagtgatca gcgacgcgaa atgattcgcc
      721 aagcagcttt taaattagtt gaaaatatta aaaatgattt aaaaccacgt gatatcgtta
      781 ctcgcgaagc aattgatgat gcatttgcac ttgatatggc tatgggtggt tcaacaaaca
      841 cggtactgca tacgttagcc attgccaatg aagctggtat tgattatgac ttagagcgca
      901 ttaatgctat tgccaaacgc acgccatatt tatcaaaaat agcacctagt tcatcgtatt
      961 caatgcatga tgtgcatgaa gctggtggcg tcccagcaat tattaatgaa ttgatgaaga
     1021 aagatggcac gttacaccca gatagaatca cagttactgg caaaacgtta cgtgaaaata
     1081 acgaaggcaa agaaattaag aactttgatg tcattcaccc tcttgatgca ccatatgatg
     1141 cacaaggcgg tttatctatc ttatttggta atatcgcccc taaaggcgca gttattaaag
     1201 ttggcggcgt tgatccatct atcaaaacat ttactgggaa agcaatttgt ttcaattcgc
     1261 atgatgaagc tgttgaagca atagacaatc gtaccgttcg tgcaggccac gtcgttgtca
     1321 ttagatatga aggacctaaa ggtggaccag gtatgcctga aatgttagca cctacttcct
     1381 ctattgttgg tcgcggctta ggtaaagatg ttgcattaat tactgatggg cgtttttccg
     1441 gtgccacaag aggtattgca gttggtcata tttcccctga agctgcatct ggtggaccaa
     1501 ttgccttaat tgaagatgga gatgagatta ctattgattt aacaaatcgt acattaaacg
     1561 taaaccagcc tgaagatgtt ctagcgcgtc gccgagaatc tttaacaccg tttaaagcga
     1621 aagtaaaaac aggttatcta gctcgttata ctgccctagt aactagcgca aatacaggtg
     1681 gcgtcatgca agtccctgag aatttaattt aatttatttt tatattggag atggttaaaa
     1741 tgtctaaaac tcaacatgaa gtaaaccaaa atattgaccc tttaaaaatg gctgaatcac
     1801 ttgaacttga acaactaaat gaaaaaactt taaatgacat gcgttcagga tcagaagtgc
     1861 tagtagaagc tctacttaaa gaaaatgtgg attatttatt cggttatcct ggtggtgccg
     1921 tactaccttt atacgacaca ttttatgatg gtaaaatcaa acatatttta gcaagacacg
     1981 aacaaggtgc tgttcatgct gcagaaggtt atgcacgtgt atctggtaaa actggcgtcg
     2041 ttgtagttac aagcggtcca ggtgcaacta atgtaatgac aggtattacg gatgcacatt
     2101 gcgactcttt acctctagtt gtattcactg gacaagttgc tacaccaggc attggtaaag
     2161 atgcattcca agaagcggat attctatcta tgacttcacc aattacaaaa caaaattatc
     2221 aagtgaaacg tgttgaagat atccctaaaa tcgtacacga agctttccat gtagctaatt
     2281 ctggacgcaa aggtcctgta gtgattgatt ttccaaaaga tatgggtgtt ttagctacaa
     2341 atgtggattt atgcgacgaa atcaatattc caggttatga agttgttaca gaaccagaaa
     2401 ataaagacat tgacactttc atctcacttt taaaagaagc gaaaaagcct gtcgtattag
     2461 ccggcgcagg tattaatcaa tcaaaatcaa atcaattatt aacacagttt gttaataaac
     2521 atcaaatccc aacagttact actttactag gtctaggtgc tgtaccttat gaggatacac
     2581 tatttttagg tatgggaggt atgcatggtt cttatgctag taacatggca ttaactgagt
     2641 gtgatttact cattaattta ggtagccgct tcgatgatag attagcaagc aaacctgatg
     2701 cctttgcacc taacgccaaa attgtacatg tagatattga tccttcagaa atcaataaag
     2761 ttattcatgt agatttaggt attattgcag actgtaaaag atttttagaa tgtttaaatg
     2821 ataaaaatgt tgagactata gaacacagtg actgggttaa acattgtcaa aataataagc
     2881 agaaacaccc atttaaactt ggtgaagaag atcaagtatt ttgtaagcca caacaaacaa
     2941 tcgaatatat cggcaaaatt acaaatggtg aagcaattgt tactacagac gtgggacaac
     3001 atcaaatgtg ggcagctcaa ttttatccat ttaaaaatca cggacaatgg gttacaagcg
     3061 gtggtttagg aacaatggga ttcggtattc cttcgtcaat tggtgccaaa ttagctaatc
     3121 ctgataaaac agtcgtatgt ttcgtcggtg acggtggttt ccaaatgaca aaccaagaaa
     3181 tggcactttt acccgaatat ggtttagatg tcaaaatcgt actaatcaat aatggaacat
     3241 taggtatggt taaacaatgg caagataagt tctttaatca acgcttctca cactcagtat
     3301 ttaatggtca acctgatttt atgaaaatgg cagaagcata tggcgtcaaa ggtttcttaa
     3361 tcgataagcc agaacaactg gaagaacaat tagatgcagc gtttgcttat caaggaccag
     3421 ttttaattga ggttcgtatt tcccctactg aagctgtaac cccaatggtt ccgagtggca
     3481 aatcaaatca tgaaatggag ggcttataat gacaagaatt cttaaattac aagttgcgga
     3541 tcaagtcagc acgctaaatc gaattacaag tgcttttgtt cgcctacaat ataatatcga
     3601 tacattacat gttacacatt ctgaacaacc tgggatttct aacatggaaa ttcaagtcga
     3661 tattcaagat gatacatcac ttcatatatt aattaaaaaa ttaaaacaac aaattaatgt
     3721 tttaacggtt gaatgctacg accttgttga taacgaagct taattttaag acaaaggcaa
     3781 tgatgcgcta attagttata gatatatcat aggctgctag ttaacatctg ccactattac
     3841 aaagttatat ttcagaattt tcgaaaaaca aaacatttaa ttatttggag gaatttatta
     3901 tgacaacagt ttattatgat caagacgtaa aaacggacgc tttacaaagc aaaaaaattg
     3961 cagtagtagg ttatggatca caaggccacg cgcatgcaca aaacttaaaa gacaatggat
     4021 atgatgtagt catcggtatt cgcccaggtc gttcttttga caaggctaaa gaagatggat
     4081 ttgatgtgtt ccctgttgca gaagcagtta agcaagctga tgtaattatg gtgctattac
     4141 ctgatgaaat tcaaggtgat gtatacaaaa acgaaattga accaaattta gaaaaacata
     4201 atgcgcttgc atttgctcat ggctttaaca ttcattttgg tgttattcaa ccaccagctg
     4261 atgttgatgt atttttagta gctcctaaag gaccgggtca tttagttaga cgtacatttg
     4321 ttgaaggttc tgctgtacca tcactatttg gtattcaaca agacgcttca ggtcaagcac
     4381 gtaatattgc tttaagttat gcaaaaggta ttggtgcaac tcgtgcaggt gttattgaaa
     4441 caacatttaa agaagaaact gagacagatt tatttggtga acaagcagta ctttgcggtg
     4501 gtgtatcgaa attaattcaa agtggctttg aaacattagt agaagcgggt tatcaaccag
     4561 aattagctta ttttgaagta ttacatgaaa tgaaattaat cgttgatttg atgtatgaag
     4621 gcggtatgga aaatgtacgt tactcaattt caaatactgc tgaatttggt gactatgttt
     4681 caggaccacg tgttatcaca ccagatgtta aagaaaatat gaaagctgta ttaactgata
     4741 tccaaaatgg taacttcagt aatcgcttta tcgaagacaa taaaaatgga ttcaaagaat
     4801 tttataaatt acgcgaagaa caacatggtc atcaaattga aaaagttggt cgtgaattac
     4861 gcgaaatgat gccttttatt aaatctaaaa gcattgaaaa ataagataga cctacaatga
     4921 ggagttgtta aatatgagta gtcatattca aatttttgat acgacactaa gagacggtga
     4981 acaaacacca ggagtgaatt ttacttttga tgaacgcttg cgtattgcat tgcaattaga
     5041 aaaatggggt gtagatgtta ttgaagctgg atttcctgct tcaagtacag gtagctttaa
     5101 atctgttcaa gcaattgcac aaacattaac aacaacggct gtatgtggtt tagctagatg
     5161 taaaaaatct gacatcgatg ctgtatatga agcaacaaaa gatgcagcga agccggtcgt
     5221 gcatgttttt atagcaacat cacctattca tcttgaacat aaacttaaaa tgtctcaaga
     5281 agacgtttta gcatctatta aagaacatgt cacatacgcg aaacaattat ttgacgttgt
     5341 tcaattttca cctgaagatg caacgcgtac tgaattacca ttcttagtga aatgtgtaca
     5401 aactgccgtt gacgctggag ctacagttat taatattcct gatacagtcg gctacagtta
     5461 ccatgatgaa tatgcacata ttttcaaaac cttaacagaa tctgtaacat cttcaaatga
     5521 aattatttat agtgctcatt gccatgacga tttaggaatg gctgtttcaa atagtttagc
     5581 tgcaattgaa ggcggtgcga gacgaattga aggcactgta aatggtattg gtgaacgagc
     5641 aggtaatgca gcacttgaag aagtcgcgct tgcactatac gttcgaaatg atcattatgg
     5701 tgctcaaact gcccttaatc tcgaagaaac taaaaaaaca tcggatttaa tttcaagata
     5761 tgcaggtatt cgagtgccta gaaataaagc aattgttggc caaaatgcat ttagtcatga
     5821 atcaggtatt caccaagatg gcgtattaaa acatcgtgaa acatatgaaa ttatgacacc
     5881 tcaacttgtt ggtgtaagca cgactgaact tccattagga aaattatctg gtaaacacgc
     5941 cttctcagag aagttaaaag cattaggtta taacattgat aaagaagcgc aaatagattt
     6001 atttaaacaa ttcaagacca ttgcggacaa aaagaaatct gtttcagata gagatattca
     6061 tgcgattatt caaggttctg agcatgagca tcaagcactt tataaattgg aaacactaca
     6121 actacaatat gtctctagcg gccttcaaag tgctgttgtt gttgttaaag ataaagaggg
     6181 tcatatttac caggattcaa gtattggtac tggttcaatc gtagcaattt acaatgcagt
     6241 tgatcgtatt ttccagaaag aaacagaatt aattgattat cgtattaatt ctgtcactga
     6301 aggtactgat gcccaagcag aagtacatgt aaatttattg attgaaggta agactgtcaa
     6361 tggctttggt attgatcatg atattttaca agcctcttgt aaagcatacg tagaagcaca
     6421 tgctaaattt gcagctgaaa atgttgagaa ggtaggtaat taattatgac ttataacatt
     6481 gttgccctac ctggtgatgg aatcggtcca gaaattttga acggatctct atcattgctt
     6541 gaaattataa gtaataaata taactttaat tatcaaatag agcaccacga atttggtggt
     6601 gcctctattg atacattcgg cgagccttta actgagaaaa ccttaaatgc gtgtaaaaga
     6661 gcagatgcta ttttactggg tgcaatcggt ggacctaaat ggacagatcc taacaatcga
     6721 ccagaacaag gattattaaa attgcgtaaa tccttaaatt tatttgcaaa tattcgcccc
     6781 actaccgttg tcaaaggcgc tagttcttta tcacctttaa aggaagaacg cgttgaaggc
     6841 acagatttag ttatagtccg tgaattgaca agtggtattt attttggaga acctagacat
     6901 tttaataatc acgaggcctt agattctctt acttatacaa gagaagaaat agaacgcatt
     6961 gttcacgtag catttaaatt ggccgcttca agacgaggaa aactaacatc agttgataaa
     7021 gaaaatgtat tagcttctag taaattgtgg cgcaaagtcg taaatgaagt aagtcaatta
     7081 tatccagaag taacagtaaa tcacttactt gttgatgctt gtagtatgca tttaatcaca
     7141 aatccaaaac aatttgacgt catcgtatgt gaaaacttat ttggcgatat tttaagtgat
     7201 gaagcttcag tgattcctgg ttcacttggt ttatcacctt ctgctagttt tagtaacgat
     7261 ggtccaagat tgtatgagcc tattcatgga tcagcaccag atattgcagg taaaaacgtt
     7321 gccaatccat ttggaatgat tctatcttta gcgatgtgtt tacgtgaaag cttaaatcaa
     7381 ccagatgctg cagatgaatt agaacaacat atttataaca tgattgaaca tgggcaaacg
     7441 acagcagatt taggcggcaa attgaatact actgatattt tcgaaattct atctcaaaaa
     7501 ttgaatcact aagggggaga tgtaaatggg tcaaacatta tttgacaagg tgtggaacag
     7561 acatgtgtta tacgggaaat tgggcgaacc gcaactatta tacattgatt tacaccttat
     7621 acatgaagtt acttctcctc aagcatttga aggacttagg cttcaaaaca gaaaattaag
     7681 acgcccagat ttaacatttg caacactcga tcacaatgtt cctactattg atattttcaa
     7741 tattaaagat gaaattgcaa acaaacaaat cacaacatta caaaaaaacg ccatagattt
     7801 tggggtgcat atttttgata tgggctctga tgaacaaggt attgttcaca tggtaggacc
     7861 tgagacagga cttacacagc ccggcaaaac aatcgtttgt ggtgactctc acacagcaac
     7921 acatggagca tttggtgcta ttgcatttgg aattggaaca agtgaagttg aacatgtttt
     7981 cgcaactcaa acgctatggc aaacaaaacc caaaaactta aaaatcgata ttaatggtac
     8041 cttaccaaca ggcgtctatg ctaaggacat tattctgcat ttaattaaaa cgtatggtgt
     8101 tgattttggt acaggctatg ctttggaatt tactggcgaa acaattaaaa acctttcaat
     8161 ggacggtcga atgactattt gtaacatggc tatcgaaggt ggtgccaaat acggcataat
     8221 ccaacctgat gatataacat ttgaatatgt taaagggaga ccatttgccg ataacttcgc
     8281 taaatcagtt gataagtggc gtgagctata ttctgatgac gacgcgatat ttgatcgtgt
     8341 aattgaactt gatgtttcaa cattagaacc acaagtgaca tggggaacta atcctgaaat
     8401 gggtgttaat ttcagtgaac cattccctga aatcaatgat atcaacgatc aacgtgcgta
     8461 tgattatatg gggttagaac caggtcaaaa agctgaagac atcgacttag ggtatgtttt
     8521 tctcggttca tgtacaaatg ctagactatc agatttgatt gaagctagtc atattgttaa
     8581 aggaaataaa gttcatccaa atattacagc tattgtcgta ccaggttctc gtacagtaaa
     8641 aaaagaagca gaaaaattag gtctagatac tatctttaaa aatgcaggat ttgaatggcg
     8701 tgaaccagga tgttcaatgt gtttaggcat gaatcctgac caagtacctg agggcgtaca
     8761 ttgtgcatct acaagtaatc gaaactttga aggacgacaa ggcaaaggtg caagaacaca
     8821 tttagtatcc cctgctatgg cagcagcagc agctattcat ggtaaatttg tggacgtaag
     8881 aaaggtggtt gtttaaatgg cagcaatcaa acctattaca acatataaag gtaaaatagt
     8941 ccctctcttc aacgacaata tcgatacaga ccaaatcatt cctaaggtac acttaaagcg
     9001 tatttcaaaa agtggctttg gtccatttgc ttttgatgaa tggcggtact tacctgatgg
     9061 ttcagataat cctgatttca atcctaacaa accacaatat aaaggggctt ctattttaat
     9121 tactggagat aattttggat gtggttcaag tcgtgaacat gctgcttggg ctcttaagga
     9181 ctatggtttt catattatta ttgcaggaag tttcagtgac atattttata tgaattgcac
     9241 taaaaatgcg atgttgccta tcgttttaga aaaaagtgct cgtgaacatc ttgcacaata
     9301 tgaggaaatt gaaatagatt taccaaatca aactgtgtca tcaccagaca agcgtttcca
     9361 ttttgaaatt gatgaaactt ggaaaaataa acttgtaaat ggcttagatg acattgcaat
     9421 caccctacaa tatgaatcat taatagaaaa atatgaaaaa tcactttaag ggagttgaat
     9481 attatgacag tcaaaacaac agtttctacg aaagatatcg atgaagcatt tttaagactt
     9541 aaagatattg tcaaagaaac acctttacaa ttagaccatt acttatctca aaagtatgat
     9601 tgtaaagttt atttaaaacg agaagattta caatgggtac gttcttttaa attaagaggt
     9661 gcttacaacg ctatttctgt tttatcagat gaagctaaaa gtaaaggtat tacatgtgcg
     9721 agtgcaggta atcatgctca aggtgttgcc tatacagcta aaaaacttaa tttaaacgct
     9781 gttatcttta tgccagtcac tacaccttta caaaaggtaa atcaagtaaa gttctttgga
     9841 aatagtaacg ttgaagttgt actcactggt gatacatttg atcactgttt agctgaagct
     9901 ttaacttata caagtgaaca tcaaatgaac tttatagatc cattcaataa tgttcataca
     9961 atttctggac aaggtacgct tgctaaagaa atgctagaac aatcaaagac tgacaatgtt
    10021 aactttgatt atttatttgc cgctattggt ggtggtggat taatttcagg tattagtact
    10081 tactttaaaa cctattcacc tactacgaaa attataggtg ttgaaccttc aggtgcaagt
    10141 agtatgtatg aatctgttgt cgtaaataat caggtagtca cattgcctaa tatcgataaa
    10201 tttgtggacg gtgcatctgt agctagagtt ggcgatatta catttgaaat tgcaaaagaa
    10261 aatgtagatg attacgttca agtagatgaa ggtgcagttt gttctacgat tttagacatg
    10321 tattcaaaac aagcaattgt agcagaacct gctggcgcat taagtgtgag tgctcttgaa
    10381 aactacaaag accacattaa gggtaaaact gttgtttgtg tcattagtgg tggtaataat
    10441 gatattaatc gtatgaagga aattgaagaa cgttctttat tatatgaaga aatgaaacat
    10501 tattttattt taaacttccc tcaacgccct ggtgcattaa gagaatttgt aaatgatgtt
    10561 ttaggcccac aagacgatat tacaaaattt gaatacttaa aaaaatcttc tcaaaataca
    10621 ggtactgtca ttattggtat tcaacttaaa gatcatgatg atttaataca actcaaacaa
    10681 cgtgtaaatc atttcgatcc ttccaatatt tatattaatg aaaataagat gttatattca
    10741 ttgttaattt aacacatagt aagaaaaaca gtcataaatt gatttctaat tgaaatcatc
    10801 ttatgactgc tttttattat actttac
//
LOCUS       DAOBBH010000082        10783 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.082, whole genome shotgun sequence.
ACCESSION   DAOBBH010000082 DAOBBH010000000
VERSION     DAOBBH010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10783
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.082"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(112..543)
                     /locus_tag="P6L01_001505"
     CDS             complement(112..543)
                     /locus_tag="P6L01_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HDP3030576.1"
                     /translation="MRRLLYSFLFYMIIGLFSGFFYRELTKAYDFTGTTQLSLVHTHT
                     LILGMFMFLILLPLEKLFKLSSYYLFNWFFYVYNIGVIVTIGMMVTKGFFQVTGKSYS
                     PEAFAGFAGIGHTGMLAGLLLLFFLLRQAILKEPRDESFKK"
     gene            complement(750..2027)
                     /locus_tag="P6L01_001506"
     CDS             complement(750..2027)
                     /locus_tag="P6L01_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDP3030577.1"
                     /translation="MNIFKNKLLWIAPIATMIILVIFSLAFYPAYNPKPKDLPIGILN
                     EDKGTTIQDKNVNIGKKLEDKLLDSDSNKIKWVKVDSEKDLEKDLKDQKIFGVAIIDK
                     DFSKDAMSKTQKVVMDSKKEEMQQKVASGEIPPQVVQQMKQKMGNQQVEVKQAKFKTI
                     VSEGSSLQGSQIASAVLTGMGDNINAQITKQSLETLTSQNVKVNAADINGLTNPVKVD
                     NEKLNKVKDHQAGGNAPFLMFMPIWIGSIVTSILLFFAFRTSNNIVVQHRIIASIGQM
                     IFAIVAAFAGSFVYIYFMQGVQGFDFDHPNRIAIFVAFAILGFVGLILGVMVWLGMKS
                     VPIFFILMFFSMQLVTLPKQMLPESYQKYVYDWNPFTHYATSVRELLYLNHHIQLNST
                     MWMFIGFMIFGAVSSLVSAIVRKHSTKRTEVPS"
     gene            2188..2811
                     /locus_tag="P6L01_001507"
     CDS             2188..2811
                     /locus_tag="P6L01_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDP3030578.1"
                     /translation="MKEDRRIRKTKSSIKQAFTKLLQEKDLEKITIRDITTRADINRG
                     TFYLHYEDKYMLLADMEDEYISELTTYTQFDLLRGSSIEDIANTFVNNILKNIFQHIH
                     DNLEFYHTILQLERTSQLELKINEHIKNNMQRYISINHSIGGVPEMYFYSYVSGATIS
                     IIKYWVMDKQPISVDELAKHVHNIIFNGPLRIMAENRLHKSNLDSLT"
     gene            2857..3801
                     /locus_tag="P6L01_001508"
     CDS             2857..3801
                     /locus_tag="P6L01_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDP3030579.1"
                     /translation="MITSFRHSEDIDKHIIKTPLDHTASWINVVEPDREEIENLMEQY
                     NIPEDFIRDPLDSEESSRIEYDEDTGYSLIIIDLPIVNSTNRSVLSFVTIPLGIIIGN
                     GIIVTVCDAENEFLENLPKRDINLKFHSRFALEILTTIADHYNRNLRLLNKSRIRIEK
                     ELKNNITNKQLFKLMEVEKSLVYFLAALKGNDTIIKKLFRLPAIKRFEEDEELLEDLI
                     IENNQAIETTELHQRILESITTSYASLLSNDMNTIMKTLTLFTVLLTLPTLVFSFFGM
                     NVPLPIDDHSYISWIIVVGISLILVVIVSIFLWRKQKL"
     gene            complement(3886..5328)
                     /locus_tag="P6L01_001509"
     CDS             complement(3886..5328)
                     /locus_tag="P6L01_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HDP3030580.1"
                     /translation="MNYKQSAEDILNAIGGEENLDAMAHCATRLRLVLNDESLVNEEA
                     LNNMDVVKGTFSTGGQYQIIIGSGTVNKVFSELEKLTGKEASTTSEVKAQSAKNMNPL
                     QRFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAKDLFFSGKSLIDVYSQFAGLAEMIN
                     VFANAPFTLLPILIGFSAAKRFGGNPFLGAVLGMILVHPSLMSAYDFPKAVEAGKAIP
                     YWDVFGLHINQVGYQGQVLPMLVAAYILASIEKGLRKVIPTVLDNLLTPLLSIFITAF
                     LTFSFVGPITRQLGYWLSDGLTWLYEFGGAIGGLIFGLLYAPIVITGMHHSFIAVETT
                     LIADATKTGGSFIFPIATMSNVAQGGAAIAAFFIIKQNKKLKGVASAAGISALLGITE
                     PAMFGVNLKLRYPFIGAIVGSGIGSAYIAFFKVKAIALGTAGLPGFISINPVHAGWLH
                     YFVGMTISFIIAITVTLILSKRKANKEVVE"
     gene            5511..5894
                     /locus_tag="P6L01_001510"
     CDS             5511..5894
                     /locus_tag="P6L01_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HDP3030581.1"
                     /translation="MKERGHIEQLWREEKYHVLLHSQQSYQMIRNALKTDLSLHQVQQ
                     MIDDALLIEPSIGSVCNAFDHMWGYFKKCANEEERKQSKLLKADFINGKIDTQTLLDF
                     LAELANKYDVQYLLQSRVLNTKRKR"
     gene            7382..9487
                     /gene="rsp"
                     /locus_tag="P6L01_001511"
     CDS             7382..9487
                     /gene="rsp"
                     /locus_tag="P6L01_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator Rsp"
                     /protein_id="HDP3030582.1"
                     /translation="MTCQLKIHHTLSTIPSRNINNIMVLFSLTSKLNITINGETKDVS
                     NYIILINHGDIYNINHGENIIELMIPVFYFYQQDDDFFNGYLDRHLLQSSNYIKSLIA
                     DLISTPTSSSLMGKNIGQSIIDTLLKEAFIRIDHEYLPNIALSNPVFIDCVNYIHDNI
                     DAHLSLKDIAMHCNISESYCSNLFVRYLSMNFKDYFTSIKLVNAINLLLSTKHSITTV
                     SELAGFNSHTNFANQFKNYLHFSPKQFRSLVSKITEPPQIHFQQDNVSQFTELISTID
                     LTAQLATNTTDIHIDDFNPKDRSQRAKVFVRFSNFNELFQFIFNEYYDINFEHLPKPV
                     VFIDDIHDIEISQTNYNLLNRCFEKLFEKNIGLAIAIKSTQQFETMKQLILTFLQGNQ
                     DYKTSKKLVKFMLVFCSNSMTAEEIHLCHLKIKNKNKEIKYSVTVDGFLETYSTVEQV
                     YDVMQRLKFHYYFIDIENSKTATHLITKNQHYHQTDTHFEQYKKFILDSGISSTQFVY
                     NNLSVSGFKYTNDGKNPIQLSDIVYHLIALLRYGGGISYQLLDDHSNYISLYNKYGSP
                     LPLMHLYKMFRPFVNEDIEITNNYVLSRKDNNYHFLLFNKINDRYMSDVKQDFIFHNE
                     LPQDSLMIIKTLNHEHGSIQHLLPISDQLVYIEKEILDELDKTNYPKTELAVQEETGR
                     TFELKLNHDEVKYICFKPS"
     gene            9558..9980
                     /locus_tag="P6L01_001512"
     CDS             9558..9980
                     /locus_tag="P6L01_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDP3030583.1"
                     /translation="MSNSQAIQAIENVLATSKVGVLSTAYNNKPNSRYMVFYNDGLTL
                     YTKTNIHSAKVKEIKDNPAAYVLLGYNDTTNRSFVEMEATIEVVTDQKVIDWLWETQD
                     KSFFSSKEDPELCVLKVTPQSVKLMNDKSLDTPIKIDL"
     gene            10244..10600
                     /locus_tag="P6L01_001513"
     CDS             10244..10600
                     /locus_tag="P6L01_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HDP3030584.1"
                     /translation="MKKKKGFGLGISLIAIMLIVCIVLVIMMMTGGKKDTYYGIMKDN
                     TTIEKMISEKDESIEKNVKLPSDSDVKVKKGDFVIVYKLADSDKIVKVKKVDHDDVPH
                     GLMMKIHDMGKMHMKH"
BASE COUNT         3788 a         1711 c         1561 g         3723 t
ORIGIN      
        1 taatcatgaa ttatcaataa ctatcccatc acaatataaa aacacacctt tcaaagtaag
       61 aaacaacaat gttcctttga aagatgtgta tattttttag ttctagttat attatttttt
      121 aaaagactca tcacgtggtt ctttaagaat tgcttgtctt aaaaggaaaa atagcaacaa
      181 taaacctgca agcatacctg tgtgcccaat acctgcaaag cctgcaaatg cttctggaga
      241 gtatgattta ccagtgactt ggaagaatcc ttttgtcacc atcataccta tagtaacgat
      301 aacacctatg ttatatacat agaagaacca gttaaataag tagtaacttg ataatttaaa
      361 taacttctcc aatggtaata atattaaaaa cataaacata cctaaaataa gtgtatgtgt
      421 gtgtacaagt gataattgtg ttgtaccagt aaaatcatat gcttttgtta gctctctata
      481 gaaaaagcca ctaaataaac ctatgatcat ataaaaaaga aatgaatata ataacctacg
      541 cattttgaat cctccctcac cttttcatta tatccgatta tacctattat aacagtgaca
      601 aaaaagcgaa gatatgacac aactgaattt ggtttagcac atagtatgac agctaaaata
      661 actttacatc aagcgctata caaataaaat agctatacca tgacgctcgc tacaaacgtc
      721 ttgtatagct attatttcta tttattaaat tatgacggca cttcagtacg ttttgtgcta
      781 tgttttctaa caatagctga aactaaactt gatacagcgc caaaaatcat aaaccctata
      841 aacatccaca ttgtactatt taattgaata tgatgattca agtataatag ttctcttaca
      901 cttgttgcat agtgtgtgaa tggattccaa tcatatacat atttttgata actttcaggc
      961 aacatttgtt taggtaacgt tacaagttgc atactaaaga acattaaaat gaagaaaatt
     1021 ggaactgact tcatacctag ccataccata acacctaaaa taaggcccac gaaaccaaga
     1081 atcgcaaatg ctacaaaaat tgcgatacga tttggatggt caaaatcaaa tccttgaacg
     1141 ccttgcatga aataaatata aacaaagcta cctgcaaatg ctgcaacaat tgcaaatatc
     1201 atctgtccaa ttgaagcaat gatacgatgt tgcacgacga tattgttact agttctaaat
     1261 gcaaagaaca ataagataga cgttacgatt gaacctatcc aaattggcat aaacattagg
     1321 aatggtgcat taccacctgc ttggtgatct ttaactttat taagtttttc attatccact
     1381 tttactggat tcgtcaaacc attgatgtcc gcggcattga ctttaacatt ttgactcgtt
     1441 aatgtttcca aactttgctt cgtaatttga gcattaatat tatcacccat accagttaac
     1501 acagctgatg caatttgtga accttgtaag cttgatcctt cacttacaat cgttttaaat
     1561 ttagcctgct taacctctac ttgttgattc cccatttttt gtttcatttg ttgaaccact
     1621 tgtggcggaa tttcacctga agcaactttt tgttgcattt cttctttttt actatccata
     1681 actacttttt gtgttttact catagcatct tttgaaaagt ctttatcaat aatagctact
     1741 ccaaagattt tttgatcttt caaatctttt tcaaggtctt tttcactatc aaccttaacc
     1801 catttaattt tattagaatc actatctaat aatttatcct ctaatttttt accaatgtta
     1861 acatttttat cttgaatcgt tgtaccttta tcctcgttta atataccaat tggtaaatct
     1921 tttggtttag gattatatgc aggataaaaa gctaaagaaa agattaccaa gataatcata
     1981 gttgctattg gcgcaatcca taataattta tttttaaata tattcatttt attaattacc
     2041 tccttattag acaatgtgtt gaatatttcg catgttgtct attataatat gtaagtggga
     2101 ataaacaata cacaatgttc atcaatacgt tcattaatag acaaatacac ccacattgtt
     2161 caataacaca aggaggttga tttatttttg aaagaagata ggcgaattag aaagacaaag
     2221 tcctccatta aacaagcgtt cactaaatta ctacaagaaa aagatttaga aaagattacc
     2281 attcgcgaca taacaacacg cgctgatatc aatagaggga cattttactt acattacgag
     2341 gataaatata tgttactcgc agatatggaa gatgagtata tttcagaact aacgacatat
     2401 actcaatttg atttgttacg cggttcttca attgaagaca ttgcgaatac ttttgtaaat
     2461 aatatactca aaaatatttt ccaacatatt catgataatt tagagtttta tcatactatt
     2521 ttacaattgg aacgcacgag tcaattagaa ttgaaaatca acgaacatat caaaaataat
     2581 atgcaacgtt atattagtat caatcattct atcggaggcg ttccagagat gtatttttac
     2641 agttacgttt ccggagcaac aatttcaatt attaaatact gggtaatgga caaacagccc
     2701 atttcagttg atgaattagc taaacatgtg cataatatta tttttaatgg tccattaaga
     2761 ataatggcag aaaaccgttt gcataaatca aaccttgatt cactaactta agtaatactc
     2821 gcttttccta aatcctttag aaaggaacta tatatcatga ttacttcatt cagacattct
     2881 gaagatatag ataaacatat tataaaaaca ccattagatc atacagcgtc atggattaat
     2941 gtagtagaac cagaccgaga agaaattgaa aatcttatgg aacaatataa tatacctgaa
     3001 gactttatac gtgacccttt ggactcagaa gaaagttccc gtattgaata tgacgaagat
     3061 actggttact cattaatcat tattgattta cctatcgtca attcaactaa tcgtagcgtt
     3121 ctatcttttg taacgattcc attaggcatt attattggca acggtattat cgtaacagtt
     3181 tgtgacgctg aaaatgaatt tttagaaaat ttacctaagc gtgatattaa tttaaaattt
     3241 cacagcagat ttgcattaga aattttaact actattgccg accattataa tcgtaactta
     3301 cgattactta ataaaagtag aattcgtatt gagaaagaac taaaaaataa cattactaac
     3361 aaacaacttt tcaaattaat ggaagttgaa aaaagtttag tatacttttt agctgcctta
     3421 aaaggtaacg atacaattat taaaaagtta ttccgtttac ctgcaattaa acgttttgaa
     3481 gaagatgaag aattactaga agatttaatc attgaaaata accaagccat cgaaacaaca
     3541 gaattacatc aacgtatctt agaaagtatc acaacatcat acgcttcctt attatctaat
     3601 gatatgaata cgattatgaa gacattaaca cttttcacgg tactattaac gttaccaacg
     3661 ctcgtattta gtttcttcgg tatgaatgtg ccgttgccaa ttgatgacca tagttatata
     3721 tcttggatta ttgtggtagg tatttcatta attcttgtcg taattgttag tattttctta
     3781 tggcgtaaac agaaattata actaaattaa aaggtatgac agcttaaagt ttcatcctat
     3841 tgcatttgca atgactgata ctttgattac tgtcatacct cttttttatt ctacaacttc
     3901 tttatttgct tttcttttag ataaaattaa agtaactgtt atagcaatga tgaatgatat
     3961 tgtcatacca acaaagtagt gtaaccatcc tgcatgtact ggattgattg aaataaatcc
     4021 tggcaatcca gcagttccta atgcgattgc tttaaccttg aagaaagcaa tatatgctga
     4081 accaatacct gatccaacga tagcgccaat aaatggatat cttagtttta agttaacacc
     4141 aaacatagcc ggttctgtaa taccaagtaa tgctgaaata cctgcggcag atgccacacc
     4201 ttttaacttc ttattttgtt taataataaa gaacgctgca attgctgcac caccttgtgc
     4261 aacattagac attgtcgcaa tcgggaatat aaatgatcca cccgttttag tggcatctgc
     4321 aattaatgtc gtttctacag ctataaagct atgatgcata cctgtaataa caatcggagc
     4381 atacaataat ccgaatatta atccaccaat tgcaccacca aattcataaa gccatgttaa
     4441 accatctgat aaccagtaac ctaattgtcg agtgattgga cctacaaatg aaaatgttag
     4501 aaatgctgtt ataaaaatag ataacaatgg tgttaacaaa ttatctaaca ccgttggaat
     4561 aactttgcgt aacccttttt caattgaggc taagatataa gctgctacaa gcataggtaa
     4621 cacttgtcct tgataaccta cttgattaat atgcaaacca aaaacatccc aatatggaat
     4681 agccttacct gcttcaactg cttttgggaa atcgtatgcg ctcattagcg atggatgaac
     4741 tagtatcata cctaatacag cacctaaaaa tggattgcca ccaaagcgtt ttgctgcact
     4801 aaatccaatt aaaattggta ataatgtaaa tggtgcattc gcaaaaacat ttatcatttc
     4861 agctaatcca gcaaattgac tatatacatc tatcaatgat ttacctgaaa agaataaatc
     4921 tttcgcagtc aaaatgttat ttaaccccat taataaacca ccagcaacga tggctggtat
     4981 aatcggaaca aagatatctg aaagcatttt tacaaatcgc tgtaacggat tcatattttt
     5041 agcagattgt gctttgactt ccgaagtggt tgatgcttct tttccagtta atttttccag
     5101 ttcactaaat actttattga ctgtaccaga cccaataata atttggtatt gtcccccagt
     5161 agaaaacgtc cctttaacta catccatatt gtttagcgcc tcttcattta ctaaactttc
     5221 atcatttaaa actaatcgta gtctcgttgc acaatgcgcc attgcatcta aattctcttc
     5281 tccgcctatc gcgttcaaaa tatcttctgc ggattgctta taattcatat atttagcctc
     5341 ctaattagaa ccggttccat ttattccact ttattgtaac cggttccaaa aatatttgca
     5401 acactctatt atcattttat ataaacacct acaccatttt tgatagcgca ttcattttta
     5461 ttgctatact atatgcatgt atattaacat taattatcgg aggtaatttt atgaaagaac
     5521 gtggccatat cgaacagctt tggcgtgagg aaaaatatca tgtattgtta cacagtcaac
     5581 agtcttacca aatgataagg aatgctttaa aaacggattt atcacttcat caagtccaac
     5641 aaatgattga tgatgctttg ttaattgaac cgagtatagg aagtgtatgt aatgcttttg
     5701 atcatatgtg gggatatttt aaaaaatgtg cgaatgaaga agaaagaaaa caatcaaaac
     5761 tacttaaagc tgattttatt aatggtaaaa tcgacaccca aactttatta gattttttag
     5821 cagaacttgc taataaatat gatgttcaat atttactaca aagtcgtgtt ttaaacacaa
     5881 aaagaaagag atagattgaa actttagttg taacggacaa atagtagtca ttggagttca
     5941 gcaattcata ctttgtacta aagtttacta taatctatct ctcaaggttt aaatgtacat
     6001 atctatatta agatgcgttt catgatgtta gaagatacca tacacattta attagttaca
     6061 gcataaatca atttataccc ttaattatta cattgtttat attttaaatt cgggggagta
     6121 attttaagta atatcttgtt gctgctaatt ctattgttaa taaattgata ttagatgtag
     6181 aaattgagtg tgaaagttaa taatagatga tttagctagt aacataatta attcatatat
     6241 agtcaaaaag ttctgttaga tactgttctt gatgaatatt ctttgatgtt aggtgttagc
     6301 tattctagat gagtttctta gatgtggatg cctgtcctga tgaagttatc tatgatgtta
     6361 atataatact tgatgttgat tcttagatgt tagtgcgatt tcaagatgct gattctttgt
     6421 tgttgcacta tcttggtgac gtttctagtt gtttatagtt atcgcatctc ttgatgtaaa
     6481 atctaagatg tttatgcatt tctagatgtt aattcttcgc tgcttaagaa ttatcttgat
     6541 ggtaaatcta gttgtgccat gcattttctc atgatgaatc taaggtgtta attcattgat
     6601 gtgtgctgtt tcttgttgct tcattatctt gatggtgaat ttcgttgtct aatgcacttt
     6661 ttcaaatgat tattctaatt gttacggcaa taactagatg gattttctta gatgttcact
     6721 tttctagatg tttatactta tcttgatgtt ttctaaacag aacttttaaa cgctttaatt
     6781 tttagtagat tgtttaaaat ttcttagttg atggttattg ttgttgattt gaataatttg
     6841 tagcgaatat tttgtaacag ggctactaag aatttttata acaagaagtg atttcaatcg
     6901 tactaatttt cagcgtttga tgacaaaagt gtatgtccaa acttaaataa tggcgataaa
     6961 atttaagtca tgggtacatt taatgttttt tgattcaaac ttccgttttg gtcaatttgc
     7021 tttttttata acatagttga atatcaataa aagattagca agttagttca aaatggctat
     7081 gtattgtttt ttgaaataca taggtttgaa atctatatta attatgcacc aaataattta
     7141 attagactct atcgaaaatt tccaaatttt gcttactatc tttctaattt tacttttata
     7201 ttgtgcacaa tgcataaaaa caagcgtttt cctaggagtt ttcattaatt tcttttattt
     7261 ttcactatta ccttttcaaa aatattcaac tataataggt atactatcca tgattttaaa
     7321 aatttccatg ttttacattt aaatgataga tttcaatata tattaggcag gagagagaaa
     7381 tatgacatgc caacttaaaa tacatcatac attatcgaca ataccaagtc gtaatattaa
     7441 taatattatg gtgctatttt cacttacaag taaactcaat attacgatta atggtgaaac
     7501 caaagacgta agtaattata ttatcttaat taaccatggt gatatttata atattaacca
     7561 cggcgaaaat ataatcgaat taatgatacc cgttttttat ttttatcaac aagatgatga
     7621 tttctttaat ggttatttag atcgtcattt attacaatca agtaattata ttaagtcatt
     7681 aattgccgat ttaatcagta caccaacaag ttcttcttta atggggaaaa atataggtca
     7741 aagtattata gacactttac ttaaagaagc ttttataaga atagatcatg aatatttacc
     7801 aaatattgct ttaagcaatc cggtgttcat agactgtgta aactatattc atgacaatat
     7861 tgatgcgcac ttgtctttaa aagatatagc gatgcattgc aacatctctg aatcatattg
     7921 ttctaatctt tttgttcgat atttgagtat gaattttaaa gattatttta caagtattaa
     7981 acttgttaac gccatcaatt tattactttc tacaaaacat tctattacaa ctgtttctga
     8041 attggccggt tttaacagtc atacaaattt tgcaaatcaa tttaagaatt acttgcattt
     8101 tagtcctaaa cagttccgtt cactcgtttc caagattact gaaccaccac aaatacactt
     8161 ccaacaagat aacgtatcac aattcactga gttaatttca acaattgatt taacagctca
     8221 actggctaca aatacaacag atattcatat agatgatttt aatccgaaag atcgaagtca
     8281 gcgtgccaaa gtatttgttc gatttagtaa tttcaatgaa ctatttcaat ttattttcaa
     8341 tgaatattac gatattaact ttgagcactt accaaaacct gtcgttttca ttgacgatat
     8401 tcacgatatt gagattagcc aaacgaatta caacctgtta aatagatgtt ttgaaaagct
     8461 tttcgaaaaa aatataggtt tagcaatcgc tattaaatca acacaacaat tcgaaacgat
     8521 gaagcagtta atactaacat ttttacaagg caatcaagac tataagacaa gcaagaaatt
     8581 agttaaattt atgctcgtat tttgctctaa ttctatgaca gcagaagaaa ttcatttatg
     8641 tcatcttaaa attaaaaaca aaaataaaga aattaaatat agtgtgacag tcgatggctt
     8701 tttagaaact tattcaaccg tggaacaagt ttatgatgtc atgcagcgtc ttaaattcca
     8761 ttattacttt attgatattg aaaattcaaa aactgcaaca catcttatca ctaaaaacca
     8821 acactatcat caaactgaca ctcattttga acagtataag aaatttattt tagattcagg
     8881 tatatcatca acacaatttg tatataataa cctgtctgta agcggattta aatatactaa
     8941 tgatggtaag aatccaattc aattatctga catagtgtat cacttaatcg cattattacg
     9001 atatggcggt ggcattagct atcaattatt agatgaccat tcaaattata tttccttgta
     9061 caacaaatat ggtagccccc taccattaat gcatttatat aaaatgttta gaccttttgt
     9121 taatgaagat attgaaatta caaataatta tgtattgagt cgtaaagata ataactacca
     9181 tttcttatta ttcaataaaa ttaatgatcg atatatgtca gacgtaaaac aagatttcat
     9241 tttccataat gaattacctc aagactcttt gatgattatt aaaacattga atcatgaaca
     9301 tggttcaatt caacatttgc ttccaataag cgatcaactt gtttatatag aaaaagaaat
     9361 tttagatgaa ttagacaaaa cgaattaccc taaaacggag cttgcagttc aagaagaaac
     9421 tggtcgtaca tttgaactca agttaaatca cgacgaggtt aaatatattt gctttaaacc
     9481 aagctaaata ctaacagtcc tcttgtgttt agtttcttac gttaaaggct atttatatca
     9541 taaggagatg atatgtaatg agtaactcac aagcaattca agcaattgaa aacgtgttag
     9601 caacgtcaaa agttggtgta ttatcaactg catataataa caaacctaat agtagatata
     9661 tggtctttta taatgatggt cttactttat atactaaaac gaatatccat tctgctaagg
     9721 tcaaagaaat taaagataat ccagcagcat atgttttgtt aggctataac gacacaacta
     9781 atcgtagttt cgttgaaatg gaagccacga tagaagtagt tactgatcaa aaagtaatcg
     9841 attggttatg ggagactcaa gataaaagct ttttcagttc aaaagaagac ccagaacttt
     9901 gtgttttaaa ggtgacacca caatctgtta aattgatgaa tgacaaatca ttagatacac
     9961 ctatcaaaat cgatttataa cacaaagtat atataggaaa taacttttat gaattctaga
    10021 tataacaatg ttaaatactt aaaataactc gctataatta aagtgtttaa tatgtttaca
    10081 atccaatttg caatgtattg tttttagaca cactttattc acatttaacg tgattagggt
    10141 gtgtcttttt aaattaattt tatgtaatat aaatactgca tttgcaaact gttgcacttt
    10201 taggtataac agaattaact acatttaagg agattgatga accatgaaaa agaaaaaagg
    10261 ttttggtctt ggtattagtt taatcgccat catgttaatt gtatgtattg tattagtaat
    10321 catgatgatg actggcggaa agaaagatac atactatgga attatgaaag ataatactac
    10381 tattgaaaaa atgattagtg aaaaagatga aagtattgaa aaaaatgtta aattaccttc
    10441 agattcagat gttaaagtta aaaaaggtga ttttgtaatt gtttataaat tagcagattc
    10501 agataaaatt gttaaagtta aaaaagttga ccatgatgac gtccctcacg gtttaatgat
    10561 gaaaattcat gacatgggca aaatgcacat gaaacactaa ttaagcttta attttgagta
    10621 atatagaaga tattaaaagc agcagtaaga tgttttctaa ttgaaaatta tcttactgct
    10681 gctttttaag aattactgtg tcagtctatt ttcggttcgc ctgcgctttt gcgcatgcat
    10741 aaaagcccct aacaacctga ggtcactgcg cttcggttcg cct
//
LOCUS       DAOBBH010000083        10542 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.083, whole genome shotgun sequence.
ACCESSION   DAOBBH010000083 DAOBBH010000000
VERSION     DAOBBH010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10542
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.083"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            3..1289
                     /gene="rseP"
                     /locus_tag="P6L01_001514"
     CDS             3..1289
                     /gene="rseP"
                     /locus_tag="P6L01_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDP3030585.1"
                     /translation="MSYLVTIIAFIIVFGVLVTVHEYGHMFFAKRAGIMCPEFAIGMG
                     PKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILD
                     DHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRD
                     RQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGL
                     QKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTERKLTK
                     VSSETKYVLGFQPASEHTLFKPIVYGFESFLKGSTLIFTAVVGMLASIFTGGFSFDML
                     NGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFR
                     KPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL"
     gene            1309..3012
                     /locus_tag="P6L01_001515"
     CDS             1309..3012
                     /locus_tag="P6L01_001515"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDP3030586.1"
                     /translation="MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLP
                     LATRVLNNITAIVRQEMERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGR
                     QFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
                     YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGE
                     DTIVYSKESDYAANIEKAEVVYEPNHKHSTVQPLEKIETPNVKTAQELADFLGRPVDE
                     IVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQDEIVNLVGANPGSLG
                     PVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKL
                     SDGSGVAHFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQPLIMGCYGIGISRTLSA
                     IVEQNHDDNGIVWPKSVTPFDLHLISINPKKDDQRELADALYAEFNTKFDVLYDDRQE
                     RAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVITNKYDNL
                     K"
     gene            3276..7586
                     /locus_tag="P6L01_001516"
     CDS             3276..7586
                     /locus_tag="P6L01_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDP3030587.1"
                     /translation="MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEF
                     HITLPQFLAHEDYLLFINAIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTA
                     LSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIF
                     ETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKAKQQDNNESAVDKCQIGK
                     PIQIENIKPIESIIEEEFKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFT
                     RKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRV
                     EFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMI
                     YGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEI
                     IDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASF
                     DMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAI
                     YDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKN
                     LFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIA
                     KYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFE
                     HDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELA
                     DRIERVVPIKDELYTPRMEGANEEIRELSYTNARKLYGEDLPQIVIDRLEKELKSIIG
                     NGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKT
                     SEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEY
                     QPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGC
                     TGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLD
                     ILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVP
                     EFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRD
                     DIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFP
                     KAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYS
                     RYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGDTLIPPFISVPG
                     LGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM
                     "
     gene            7876..8343
                     /gene="rimP"
                     /locus_tag="P6L01_001517"
     CDS             7876..8343
                     /gene="rimP"
                     /locus_tag="P6L01_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDP3030588.1"
                     /translation="MSKITEQVEVIVQPIMEDLNFELVDVEYVKEGRDHFLRISIDKE
                     GGVDLNDCTLASEKISEAMDANDPIPEMYYLDVASPGAERPIKKEQDFQNAITKPVFV
                     SLYVPIEGEKEWLGILQEVNNETIVVQVKIKARTKDIEIPRDKIAKARHAVMI"
     gene            8364..9539
                     /gene="nusA"
                     /locus_tag="P6L01_001518"
     CDS             8364..9539
                     /gene="nusA"
                     /locus_tag="P6L01_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDP3030589.1"
                     /translation="MSSNELLLATEYLEKEKKIPRAVLIDAIEAALITAYKKNYDSAR
                     NVRVELNMDQGTFKVIARKDVVEEVFDDRDEVDLSTALVKNPAYEIGDIYEEDVTPKD
                     FGRVGAQAAKQAVMQRLRDAEREILFEEFIDKEEDILTGIIDRVDHRYVYVNLGRIEA
                     VLSEAERSPNEKYIPNERIKVYVNKVEQTTKGPQIYVSRSHPGLLKRLFEQEVPEIYD
                     GTVIVKSVAREAGDRSKISVFSENNDIDAVGACVGAKGARVEAVVEELGGEKIDIVQW
                     NEDPKVFVKNALSPSQVLEVIVDETNQSTVVVVPDYQLSLAIGKRGQNARLAAKLTGW
                     KIDIKSETDAREAGIYPVVEAEKVTEEDIALEDADTTESTEEVNDVSVETNVEKESE"
     gene            9560..9844
                     /locus_tag="P6L01_001519"
     CDS             9560..9844
                     /locus_tag="P6L01_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDP3030590.1"
                     /translation="MKKKKIPMRKCILSNEMHPKKDMIRVVVNKEGEIFADVTGKKQG
                     RGAYVSKDVAMVEKAQQKEILEKYFKASKEQLDPVYKEIIRLIYREEIPK"
     gene            9841..10158
                     /locus_tag="P6L01_001520"
     CDS             9841..10158
                     /locus_tag="P6L01_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ family RNA-binding protein"
                     /protein_id="HDP3030591.1"
                     /translation="MSIDQILNFLGLAMRAGKVKTGESVIVNEIKKGNLKLVIVANDA
                     SDNTAKLITDKCKSYKVPFRKFGNRNELGIALGKGERVNVGITDPGFAKKLLSMIDEY
                     HKE"
     gene            10163..>10542
                     /locus_tag="P6L01_001521"
     CDS             10163..>10542
                     /locus_tag="P6L01_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HDP3030592.1"
                     /translation="MSKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIK
                     ALDKKFKKEQKNDNKQSTQNNHQKSNNQNQNKGQQKDNKKNQQQNNKGNKGNKKNNRN
                     NKKNNKNNKPQNQPAAPKEIPSKVT"
BASE COUNT         3842 a         1460 c         2110 g         3130 t
ORIGIN      
        1 gagtgagcta tttagttaca ataattgcat ttattattgt ttttggtgta ctagtaactg
       61 ttcatgaata tggacatatg ttttttgcga aaagagcagg cattatgtgt ccagaatttg
      121 cgatcggtat ggggccaaaa atttttagtt ttagaaaaaa tgaaacactt tacactatta
      181 ggttattgcc tgttggtgga tatgttcgta tggcaggaga tggcttagaa gagccaccag
      241 tcgagcccgg tatgaacgtt aaaattaaac tgaatgaaga aaatgaaata acacatatca
      301 tattagatga tcatcataag tttcaacaaa ttgaagcgat cgaagttaaa aaatgtgatt
      361 ttaaggatga cttattcata gaaggtatca ctgcttatga taatgaaaga catcatttta
      421 aaattgctag aaagtctttc tttgttgaaa atggtagctt agttcaaatt gctccgagag
      481 acagacaatt tgcacataaa aagccatggc cgaaattttt aacattattt gcgggaccgt
      541 tatttaactt tatattagct ttagtcctat ttattggtct tgcatattat caaggtacac
      601 ctacgtctac tgtagaacaa gtcgcagata agtatccagc tcaacaagca ggattacaaa
      661 aaggtgataa aattgttcaa attggcaaat ataaaatatc tgaatttgat gatgttgata
      721 aggcgttaga taaagttaaa gataataaga cgactgttaa atttgaacgt gatggtaaaa
      781 cgaagtcagt tgaattaaca cctaaaaaga ctgaaagaaa actgactaaa gtaagttcag
      841 agacgaagta tgttctcgga ttccaaccag cgagtgaaca tacacttttc aaaccaattg
      901 tatatggatt tgaaagcttt ttgaaaggta gtacactaat ttttacagct gtagtcggta
      961 tgttggctag tatatttacg ggcggattct catttgatat gttaaatggt ccggttggta
     1021 tttatcataa cgtcgactca gttgttaaag cgggtatcat tagcttaatt ggttacactg
     1081 cgttattaag tgtaaactta ggtattatga atttaattcc tattcctgca ctagacggtg
     1141 gtcgtatttt atttgttata tatgaagcga ttttcagaaa accagttaat aaaaaagcgg
     1201 aaacaacgat tattgctatt ggtgccattt tcatggtcgt tattatgata ttagtaacgt
     1261 ggaatgatat tcgacgatat ttcttataat ttaggaggat aaataattat gaagcaatcc
     1321 aaagttttta taccaacgat gcgtgatgtg ccatcagaag cagaagcaca aagtcatcgt
     1381 ttattattga aatcgggttt gataaaacaa agtacaagtg ggatttatag ttatttaccg
     1441 ctagcaacac gtgtgttaaa taatattact gcaattgtgc gacaagaaat ggaacgtatc
     1501 gattctgttg aaattttaat gccagcgtta caacaagctg aattatggga agaatcagga
     1561 cgttggggtg catatggccc agaattaatg cgtttacaag atagacatgg aagacaattt
     1621 gcattaggtc caacacatga agaattagtt acatcaatag taagaaatga attgaaatca
     1681 tacaaacaat taccgatgac attattccaa attcaatcta aattccgtga tgaaaagaga
     1741 ccacgttttg gtttacttcg tgggcgtgaa tttattatga aagatgcgta ttcattccat
     1801 gctgacgagg catcattaga tcaaacgtat caagatatgt atcaagcgta tagccgtatt
     1861 tttgagagag ttggcattaa tgctagacca gttgtagcag attcaggtgc tataggcggt
     1921 agccatacac atgaatttat ggcattaagt gctatcggtg aggatacaat cgtttacagt
     1981 aaagaaagtg actatgctgc taatatcgaa aaagcagaag tcgtttacga accaaatcat
     2041 aagcattcta ctgtgcaacc tttagaaaaa attgaaacac caaatgttaa gactgcacaa
     2101 gaattggcag acttcttagg tagaccagta gatgaaatcg ttaaaacgat gattttcaaa
     2161 gttgatggcg aatatattat ggttttagtg cgtggccatc atgaaattaa tgacattaaa
     2221 ttaaaatctt atttcggcac agataatatt gaattagcaa cacaagacga aattgttaat
     2281 ttagttggtg caaatccggg ttcactaggt cctgttattg ataaagaaat caaaatttat
     2341 gcagataatt ttgtgcaaga tttaaataat ttagttgtcg gtgctaacga agatggctat
     2401 cacttaatta atgtaaatgt aggtagagac ttcaacgttg atgaatatgg cgatttccgt
     2461 tttattttag aaggcgaaaa gttaagtgat ggttcaggcg ttgcacattt tgctgaaggt
     2521 attgaagttg gtcaagtatt caaattgggt actaagtatt cagaatcaat gaatgctaca
     2581 ttcttagata accaaggaaa agctcaacct ttaattatgg gctgttacgg tattggaatt
     2641 tctagaacgc taagtgcgat tgttgaacaa aatcacgatg ataatggaat tgtttggcct
     2701 aaatcagtta ctccatttga tttacattta atttctatta atcctaagaa agatgatcaa
     2761 cgagagctag cagatgcact atatgctgaa tttaatacta aatttgatgt gttgtacgat
     2821 gatcgtcagg aacgtgcagg tgtcaaattt aatgatgccg atttaattgg tttaccactg
     2881 cgaattgttg ttggtaaacg tgcatcggaa ggtattgtag aagttaaaga acgtttaaca
     2941 ggtgatagcg aagaagttca cattgatgac ttaatgactg tcattacaaa taaatatgat
     3001 aacttaaaat aattaagatc gaatgaatta taagagtagg aaaaagctga aagaaatctg
     3061 atgcttatgt cctgctctta ttatttttga tataatgatt attcgatgaa aaatgactga
     3121 agacatagta taattaaaga taaatttgtt ttaacaatat tatgattagc caaatataaa
     3181 gcatttaatt ttctatcatt actatgctca cataatctaa atattgttcg aacacgtaaa
     3241 agtaatttct atttaaggtg gtaattgtct tggcaatgac agagcaacaa aaatttaaag
     3301 tgcttgctga tcaaattaaa atttcaaatc aattagatgc tgaaatttta aattcaggtg
     3361 aactgacacg tatagatgtt tctaacaaaa acagaacatg ggaatttcat attacattac
     3421 cacaattctt agctcatgaa gattatttat tatttataaa tgcaatagag caagagttta
     3481 aagatatcgc caacgttaca tgtcgtttta cggtaacaaa tggcacgaat caagatgaac
     3541 atgcaattaa atactttggg cactgtattg accaaacagc tttatctcca aaagttaaag
     3601 gtcaattgaa acagaaaaag cttattatgt ctggaaaagt attaaaagta atggtatcaa
     3661 atgacattga acgtaatcat tttgataagg catgtaatgg aagtcttatc aaagcgttta
     3721 gaaattgtgg ttttgatatc gataaaatca tattcgaaac aaatgataat gatcaagaac
     3781 aaaacttagc ttctttagaa gcacatattc aagaagaaga cgaacaaagt gcacgattgg
     3841 caacagagaa acttgaaaaa atgaaagctg aaaaagcgaa acaacaagat aacaacgaaa
     3901 gtgctgtcga taagtgtcaa attggtaagc cgattcaaat tgaaaatatt aaaccaattg
     3961 aatctattat tgaggaagag tttaaagttg caatagaggg tgtcattttt gatataaact
     4021 taaaagaact taaaagtggt cgtcatatcg tagaaattaa agtgactgac tatacggact
     4081 cattagtttt aaaaatgttt actcgtaaaa acaaagatga tttagaacat tttaaagcgc
     4141 taagtgtagg taaatgggtt agggctcaag gtcgcattga agaagataca tttattagag
     4201 atttagttat gatgatgtct gatattgaag agattaaaaa agcgacaaaa aaagataagg
     4261 ctgaagaaaa gcgtgtagaa ttccacttgc atactgcaat gagccaaatg gatggtatac
     4321 ccaatattgg tgcgtatgtt aaacaggcag cagactgggg acatccagcc attgcggtta
     4381 cagaccataa tgttgtgcaa gcatttccag atgctcacgc agcagcggaa aaacatggca
     4441 ttaaaatgat atacggtatg gaaggtatgt tagttgatga tggtgttccg attgcataca
     4501 aaccacaaga tgtcgtatta aaagatgcta cttatgttgt gttcgacgtt gagacaactg
     4561 gtttatcaaa tcagtatgat aaaatcatcg agcttgcagc tgtgaaagtt cataacggtg
     4621 aaatcatcga taagtttgaa aggtttagta atccgcatga acgattatcg gaaacgatta
     4681 tcaatttgac gcatattact gatgatatgt tagtagatgc ccctgagatt gaagaagtac
     4741 ttacagagtt taaagaatgg gttggcgatg cgatattcgt agcgcataat gcttcgtttg
     4801 atatgggctt tatcgatacg ggatatgaac gtcttgggtt tggaccatca acgaatggtg
     4861 ttatcgatac tttagaatta tctcgtacga ttaatactga atatggtaaa catggtttga
     4921 atttcttagc taaaaaatat ggcgtagaat taacgcaaca tcaccgtgcc atttatgata
     4981 cagaagcaac agcttacatt ttcataaaaa tggttcaaca aatgaaagaa ttaggtgtat
     5041 taaatcataa cgaaatcaac aaaaaactca gtaatgaaga tgcatataaa cgtgcaagac
     5101 ctagccatgt cacattaatt gtacaaaacc aacaaggtct taaaaatcta tttaaaattg
     5161 taagtgcatc attggtgaag tatttctacc gtacacctcg aattccacgt tcattgttag
     5221 atgaatatcg tgagggatta ttggtaggta cagcgtgtga tgaaggtgaa ttatttacgg
     5281 cagttatgca gaaggaccag agccaagttg aaaaaattgc caaatattat gattttattg
     5341 aaattcaacc accggcactt tatcaagatt taattgatag agagcttatt agagatactg
     5401 aaacattaca tgaaatttat caacgtttaa tacatgcagg tgacacagcg ggtatacctg
     5461 ttattgcgac aggaaatgca cactatttgt ttgaacatga tggtatcgca cgtaaaattt
     5521 taatagcatc acaacccggc aatccactta atcgctcaac tttaccggaa gcacatttta
     5581 gaactacaga tgaaatgtta aacgagtttc attttttagg tgaagaaaaa gcgcatgaaa
     5641 ttgttgtgaa aaatacaaac gaattagcag atcgaattga acgtgttgtt cctattaaag
     5701 atgaattata cacaccgcgt atggaaggtg ctaacgaaga aattagagaa ctaagttata
     5761 caaatgcgcg taaactgtat ggtgaagacc tgcctcaaat cgtaattgat cgattagaaa
     5821 aagaattaaa aagtattatc ggtaatggat ttgcggtaat ttacttaatt tcgcaacgtt
     5881 tagttaaaaa atcattagat gatggatact tagttggttc ccgtggttca gtaggttcta
     5941 gttttgtagc gacaatgact gagattactg aagtaaaccc gttaccgcca cactatattt
     6001 gtccgaactg taaaacgagt gaatttttca atgatggttc agtaggatca ggattcgatt
     6061 tacctgataa gacgtgtgaa acttgtggag cgccacttat taaagaagga caagatattc
     6121 cgtttgaaac atttttagga tttaagggag ataaagttcc tgatatcgac ttaaacttta
     6181 gtggtgaata tcaaccgaat gcccataact acacaaaagt attatttggt gaggataaag
     6241 tattccgtgc aggtacaatt ggtaccgttg ctgaaaagac tgcttttggt tatgttaaag
     6301 gttatttgaa tgatcaaggc atccacaaaa gaggcgctga aatagatcga ctcgttaaag
     6361 gatgtacagg tgttaaacgt acaactggac agcatccagg aggtattatt gtagtacctg
     6421 attacatgga tatttatgat tttacgccta tacaatatcc tgccgatgat caaaattcag
     6481 catggatgac gacacatttt gatttccatt ctattcatga taatgtatta aaacttgata
     6541 tacttggaca cgatgatcca acaatgattc gtatgcttca agatttatcg ggcattgatc
     6601 caaaaacgat acctgtagat gacaaagaag tcatgcaaat atttagtaca cctgaaagtt
     6661 taggtgttac tgaagatgaa attttatgta aaacaggtac gtttggggtt ccagaattcg
     6721 gtacagggtt cgtgcgtcaa atgttagaag atacaaagcc aacaacattt tctgaattag
     6781 ttcaaatctc aggattatct catggtacag atgtgtggtt aggcaatgct caagaattaa
     6841 ttaaaaccgg tatatgtgat ttatcaagtg taattggttg tcgtgatgat atcatggttt
     6901 atttaatgta tgctggttta gaaccatcaa tggcttttaa aataatggag tcagtacgta
     6961 aaggtaaagg tttaactgaa gaaatgattg aaacgatgaa agaaaatgaa gtgccggatt
     7021 ggtatttaga ttcatgtctt aaaattaagt acatgttccc taaagcccat gcagcagcat
     7081 acgttttaat ggcagtacgt atcgcatatt tcaaagtaca tcatccactt tattactatg
     7141 catcttactt tacaattcgt gcgtcagatt ttgatttaat cacgatgatt aaagataaaa
     7201 caagcattcg aaatactgta aaagacatgt attctcgcta tatggatcta ggtaaaaaag
     7261 aaaaagacgt attaacagtc ttggaaatta tgaatgaaat ggcgcatcga ggttatcgaa
     7321 tgcaaccgat tagtttagaa aagagtcagg cgttcgaatt tatcattgaa ggcgatacac
     7381 ttattccgcc gttcatatca gtgcctgggc ttggcgaaaa cgttgcgaaa cgaattgttg
     7441 aagctcgtga cgatggccca tttttatcaa aagaagattt aaacaaaaaa gctggattat
     7501 ctcagaaaat tattgagtat ttagatgagt taggctcatt accgaattta ccagataaag
     7561 ctcaactttc gatatttgat atgtaaaatg aaataatcaa ggtatttatt taatgcgtat
     7621 ggcgtagtta aagaaataca aaattggtgc tggacataaa attatgcctg tatttctttt
     7681 caacgtctta cgagtctatt cgaatgtaat ggtgaaataa atgaacaaac ttttacaaga
     7741 atctctgatt aatagtgaag tcatttgttt caagcataaa cttatgctat aattaagttg
     7801 cttaaaaatt agtgaactca ggcagaagag tgggagattc ccgctctttt ctatttgcca
     7861 aaaagggagg cctgtatgag taaaattaca gaacaagtag aagtgattgt tcaaccaatt
     7921 atggaagact tgaattttga acttgtagac gttgaatatg tcaaagaggg tagagatcat
     7981 tttcttagaa tctctattga taaagaaggt ggcgtagatt taaatgattg tacgctagct
     8041 tctgaaaaaa taagtgaagc tatggatgca aatgatccta ttcctgaaat gtattattta
     8101 gacgtagcgt cacctggtgc agaacgtcca attaaaaaag aacaagattt ccaaaatgca
     8161 ataactaaac ctgtatttgt ttctttatat gtaccaattg aaggtgaaaa ggaatggtta
     8221 ggcattttac aagaagtcaa taatgaaaca attgtagtac aagttaaaat caaagcaaga
     8281 acgaaagata tagagatacc gagagacaaa atagcaaaag cacgtcacgc agttatgatt
     8341 taacgtgatg aggaggaaaa aacgtgtcaa gtaatgaatt attattagct actgagtatt
     8401 tagaaaaaga aaagaagatt cctagagcag tattaattga tgctattgaa gcagctttaa
     8461 ttactgcata caaaaagaat tatgatagtg caagaaatgt ccgtgtggaa ttaaatatgg
     8521 atcaaggtac tttcaaagtt atcgctcgta aagatgttgt tgaagaagta tttgacgaca
     8581 gagatgaagt ggatttaagt acagcgcttg ttaaaaaccc tgcatatgaa attggtgata
     8641 tatacgaaga agatgtaaca cctaaagatt ttggtcgtgt aggtgctcaa gcagcgaaac
     8701 aagcagtaat gcaacgtctt cgtgatgctg aacgtgaaat tttatttgaa gaatttatag
     8761 acaaagaaga agacatactt actggaatta ttgaccgtgt tgaccatcgt tatgtatatg
     8821 tgaatttagg tcgtatcgaa gctgttttat ctgaagcaga aagaagtcct aacgaaaaat
     8881 atattcctaa cgaacgtatc aaagtatatg ttaacaaagt ggaacaaacg acaaaaggtc
     8941 ctcaaatcta tgtttctcgt agccatccag gtttattaaa acgtttattt gaacaagaag
     9001 ttccagaaat ttacgatggt actgtaattg ttaaatcagt agcacgtgaa gctggcgatc
     9061 gctctaaaat tagtgtcttc tctgaaaaca atgatataga tgctgttggt gcatgtgttg
     9121 gtgctaaagg cgcacgtgtt gaagctgttg ttgaagagct aggtggcgaa aaaatcgaca
     9181 tcgttcaatg gaatgaagat ccaaaagtat ttgtaaaaaa tgctttaagc ccttctcaag
     9241 ttttagaagt tattgttgat gaaacaaatc aatctacagt agttgttgtt cctgattatc
     9301 aattgtcatt agcgattggt aaaagaggac aaaacgcacg tctagctgct aaattaaccg
     9361 gctggaaaat tgatattaaa tcagaaacag atgcgcgtga agcgggtatc tatccagtag
     9421 ttgaagctga aaaagtaact gaagaagata ttgctttaga agatgctgac acaacagaat
     9481 caaccgaaga ggtaaatgat gtttcagttg aaacaaatgt agagaaagaa tctgaataat
     9541 aggttggagt gaagtatcta tgaaaaagaa aaaaattccg atgcgaaaat gtattctttc
     9601 aaatgaaatg catcccaaaa aagatatgat tcgtgttgtt gttaataaag aaggcgaaat
     9661 ctttgcggat gttactggaa agaaacaagg ccgtggcgca tatgtttcta aagatgttgc
     9721 tatggttgaa aaagcacaac aaaaagaaat tttagaaaaa tattttaaag catctaaaga
     9781 gcaattggat cctgtttaca aagaaattat tagattaatt tatagagaag agattccaaa
     9841 atgagtatag atcaaatatt aaacttttta ggattagcaa tgagagctgg taaagtaaaa
     9901 acaggtgaat cagtcattgt taatgagatt aaaaaaggaa atttgaagct cgttattgtt
     9961 gcaaatgatg cgtctgataa tacagctaaa ttaattacag ataaatgtaa gagttacaaa
    10021 gttccattca gaaagtttgg aaatcgaaat gaattgggaa tagcacttgg aaaaggtgag
    10081 cgtgttaatg tagggattac tgacccaggc tttgctaaaa agttgctatc aatgatagat
    10141 gaatatcata aggagtgatt atatgagtaa acaaagaatt tacgaatatg cgaaagaatt
    10201 aaatctaaag agtaaagaga ttatagatga gttaaaaagc atgaatatag aggtttcaaa
    10261 tcatatgcaa gctttggaag atgaccaaat taaagcatta gataaaaagt tcaaaaaaga
    10321 acaaaagaac gacaataaac aaagcactca aaataatcac caaaaatcaa acaatcaaaa
    10381 ccaaaataaa gggcaacaaa aagataacaa aaagaatcaa caacaaaata ataaaggcaa
    10441 caaaggcaat aaaaagaata atagaaataa taagaaaaat aacaagaata ataaaccaca
    10501 aaatcaacca gctgctccaa aagaaatacc atcaaaagtg ac
//
LOCUS       DAOBBH010000084        10501 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.084, whole genome shotgun sequence.
ACCESSION   DAOBBH010000084 DAOBBH010000000
VERSION     DAOBBH010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10501
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.084"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            5..241
                     /locus_tag="P6L01_001522"
     CDS             5..241
                     /locus_tag="P6L01_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030593.1"
                     /translation="MLEIFLIGFGVYLFYRIAIIFLKSKKTIHTNIYEMLMLATIFMI
                     STFAYKHQKTHILIAFLVMFFMSKLKQVQGSYEE"
     gene            238..444
                     /locus_tag="P6L01_001523"
     CDS             238..444
                     /locus_tag="P6L01_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HDP3030594.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAKEKY
                     EKQVKRDAVIKVGQLFENIRECGK"
     gene            441..827
                     /locus_tag="P6L01_001524"
     CDS             441..827
                     /locus_tag="P6L01_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030595.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGITALLLVVALPIYTVASYQNK
                     EVHQGTITDKYNKRQDKEDKFYIVLDDKQVIENSDLFFKGKFDSADIQARLKVGDKVK
                     VKTIGYRIHFLNLYPVLYEVKKVDKK"
     gene            824..973
                     /locus_tag="P6L01_001525"
     CDS             824..973
                     /locus_tag="P6L01_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDP3030596.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYVFR
                     AELSE"
     gene            973..1173
                     /locus_tag="P6L01_001526"
     CDS             973..1173
                     /locus_tag="P6L01_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HDP3030597.1"
                     /translation="MWITMTIVFAILLLVCISINSDRAREIQALRYMNDYLLDEVVKT
                     KGYNGLKEYRIELKRMNNDIKK"
     gene            1201..1617
                     /locus_tag="P6L01_001527"
     CDS             1201..1617
                     /locus_tag="P6L01_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDP3030598.1"
                     /translation="MIKIEKHDIKKLEEYIQHIDNYRRELKMREYELLESHEPDNAGA
                     GKSNLPGNPIERCAIKKFSDNRYNTLRNIVNGVDRLIDESDEDTLELLRFRYWDCPIG
                     CYEWEDIAHYFGTSKTSILRRRNALIDKLAKYIGYV"
     gene            1849..2148
                     /locus_tag="P6L01_001528"
     CDS             1849..2148
                     /locus_tag="P6L01_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDP3030599.1"
                     /translation="MMTKDERIRFYKSKEWQTTRKRVLERDNYECQQCKRDGKLTTYD
                     KSKRKSLDVDHILSLEHHPEFAHDLNNLETLCIKCHNKKEKRFIKKENKWKDEKW"
     gene            2278..2622
                     /locus_tag="P6L01_001529"
     CDS             2278..2622
                     /locus_tag="P6L01_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030600.1"
                     /translation="MEIIVDENLVLKEKERLQVLYKDIPSNKLKVVDGLIIQAARLRV
                     MLDYMWEDIKEKGDYDLFTQSEKAPPYERERPVAKLFNARDAAYQKIIKQLSDLLPEE
                     KEDTETPSDDYL"
     gene            2619..4280
                     /locus_tag="P6L01_001530"
     CDS             2619..4280
                     /locus_tag="P6L01_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDP3030601.1"
                     /translation="MISNKYVDEYINLWKQGKIILNKERIDLFNYLQKHIYSRDDVYF
                     DEQKIEDCIKFIEKWYFPTLPFQRFIIANIFLIDKNTDEAFFTEFAIFMGRGGGKNGL
                     ISAISDFLSTPLHGVKEYHISIVANSEDQAKTSFDEIRTVLMDNKRNKTGKTPKAPYE
                     VSKAKIINRATKSVIRYNTSNTKTKDGGREGCVIFDEIHYFFGPEMVNVKRGGLGKKK
                     NRRTFYISTDGFVREGYIDAMKHKIASVLSGKVKNSRLFAFYCKLDDPKEVDDRQTWE
                     KANPMLHKPLSEYAKTLLSTIEEEYNDLPFNRSNKPEFMTKRMNLPEVDLEKVIAPWK
                     EILATNREIPNLDNQMCIGGLDFANIRDFASVGLLFRKNDDYIWLGHSFVRQGFLDDV
                     KLEPPIKEWEKMGLLTIVDDDVIEIEYIVDWFLKAREKYGLEKVIADNYRTDIVRRAF
                     EDAGIKLEVLRNPKAIHGLLAPRIDTMFAKHNVIYGDNPLMRWFTNNVAVKVKPDGNK
                     EYIKKDENRRKTDGFMAFVHALYRADDIVDKDMSKALDALMSIDF"
     gene            4296..5483
                     /locus_tag="P6L01_001531"
     CDS             4296..5483
                     /locus_tag="P6L01_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDP3030602.1"
                     /translation="MSILEKIFKTRKDITYMLDLDMIEDLSQQAYVKRLAIDSCIEFV
                     ARAVAQSHFKVLEGNRIQKNDVYYKLNIKPNTDLSSDSFWQQVIYKLIYDNEVLIVVS
                     DSKELLIADSFYREEYALYDDIFKDVTVKDYTYQRTFTMQEVIYLKYNNNKVTHFVES
                     LFEDYGKIFGRMIGAQLKNYQIRGILKSASSAYDEKNIEKLQAFTNKLFNTFNKNQLA
                     IAPLIEGFDYEELSNGGKNSNMPFSELSELMRDAIKNVALMIGIPPGLIYGETADLEK
                     NTLVFEKFCLTPLLKKIQNELNAKLITQSMYLKDTRIEIVGVNKKDPLQYAEAIDKLV
                     SSGSFTRNEVRIMLGEEPSDNPELDEYLITKNYEKANSGENDEKEKDENTLKGGDEDE
                     SGD"
     gene            5467..6204
                     /locus_tag="P6L01_001532"
     CDS             5467..6204
                     /locus_tag="P6L01_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDP3030603.1"
                     /translation="MKAEIKGVIVSNEDKWVYEMLGMDSTCPKDVLTQLEFSDEDVDI
                     IINSNGGNLVAGSEIYTHLRAHKGKVNVRITAIAASAASLIAMAGDHIEMSPVARMMI
                     HNPSSIAQGEAKDLNHAAETLEHVGQIMAEAYAVRAGKNKQELVEMMARETWLNADEA
                     IEQGFADSKMFENDNMQIVANNTQVLSKDVLNRVTALVSKTPEVNIDIDAIANKVIEK
                     INMKEKESEIDVADSKVSANGFSRFLF"
     gene            6228..7373
                     /locus_tag="P6L01_001533"
     CDS             6228..7373
                     /locus_tag="P6L01_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDP3030604.1"
                     /translation="MTINLSETFANAKNEFINAVNNGEPQERQNELYGDMINQLFEET
                     KLQAKAEAERVSSLPKSAQSLSANQRSFFMDINKNVNYKEEKLLPEETIDRIFEDLTT
                     NHPLLADLGIKNAGLRLKFLKSETSGVAVWGKIYGEIKGQLDAAFSEETAIQNKLTAF
                     VVLPKDLNDFGPAWIERFVRVQIEEAFAVALETAFLKGTGKDQPIGLNRQVQKGVSVT
                     EGAYPEKEEQGTLTFANPRATVNELTQVFKYHSTNEKGKSVAVKGNVTMVVNPSDAFE
                     VQAQYTHLNANGVYVTALPFNLNVIESTVQEAGKVLTYVKGLYDGYLAGGINVQKFKE
                     TLALDDMDLYTAKQFAYGKAKDNKVAAVWKLDLKGHKPALEGTEETL"
     gene            7393..7677
                     /locus_tag="P6L01_001534"
     CDS             7393..7677
                     /locus_tag="P6L01_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030605.1"
                     /translation="MVKFKVVRAFKDIEHNQHKYKVGELYPAEGYNNPRVELLTNQIK
                     NKYDKVYIVPLDKLTKQELLELCESLQKKASSSMVKSEIVDLLNGEDNDD"
     gene            7667..7951
                     /locus_tag="P6L01_001535"
     CDS             7667..7951
                     /locus_tag="P6L01_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HDP3030606.1"
                     /translation="MTIDDLLVKFKSLERIDHNSEDEYLKQLLKMSYERIKNQCGVFE
                     LENLIGQELILIRARYAYQDLLEHFNDNYRPEIIDFSLSLMEVSEDEESV"
     gene            7935..8297
                     /locus_tag="P6L01_001536"
     CDS             7935..8297
                     /locus_tag="P6L01_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HDP3030607.1"
                     /translation="MKKVFKKPRITTKRLNTRVHFYKYTENNGPEAGEKEEKLLYSCW
                     ASIDGVWLRELEQAISNGTQNDIKLYIRDPQGDYLPSEEHYLEIESRYFKNRLNIKQV
                     SPDLDNKDFIMIRGGYSS"
     gene            8294..8698
                     /locus_tag="P6L01_001537"
     CDS             8294..8698
                     /locus_tag="P6L01_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDP3030608.1"
                     /translation="MSVKVIGDKALERELEKRFGIKEMVKVQDKALIAGAKVIVEEVK
                     KQLKPSKDTGALINEVSFSKPEWINGKRTITVHWRGSKDRYKIVHLIEYGHVQKGTGK
                     FIKPKAMGGVNRAIRQGQNKYFETLKRELKKL"
     gene            8695..9102
                     /locus_tag="P6L01_001538"
     CDS             8695..9102
                     /locus_tag="P6L01_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030609.1"
                     /translation="MIDILYKVHEVISQDRIIREHVNINNIKFNKYPNVKDTDVPFIV
                     IDDIDDPIPTTYTDGDECAYSYIVQIDVFVKYNDEYNARIIRNKISNRIQKLLWSELK
                     MGNVSNGKPEYIEEFKTYRSSRVYEGIFYKEEN"
     gene            9103..9747
                     /locus_tag="P6L01_001539"
     CDS             9103..9747
                     /locus_tag="P6L01_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDP3030610.1"
                     /translation="MAVKHASAPKAYINITGLGFAKLTKEGAELKYSDITKTRGLQKI
                     GVETGGDLKTAYADGGPIESGNTDGEGKISLQMHAFPKEIRKIVFNEEYDEDGVYKET
                     QGKQNNYVAIWFRQERRDGTFRTVLLPKVMFTNPKIDGETAEKDWDFSSEEVEGEALF
                     PLVDNKKSVRKYIFDSANMTNHDGDGEKGEEAFLKKILGEEYTGNVTEDSEETL"
     gene            9789..10013
                     /locus_tag="P6L01_001540"
     CDS             9789..10013
                     /locus_tag="P6L01_001540"
                     /inference="COORDINATES: protein motif:HMM:NF014427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HDP3030611.1"
                     /translation="MPDSIKTLKVGDTYDLNVVVEPSNQSKLLKYTTDQTNIVSINSD
                     GQVTAEAQGIATVKATVGNMSDTITINVEA"
     gene            10063..10413
                     /locus_tag="P6L01_001541"
     CDS             10063..10413
                     /locus_tag="P6L01_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030612.1"
                     /translation="MAKLKRNIIQLVEDPKANEIKLQTYLTPHFISFEIVYEAMDLID
                     DIEDENSTMKPREIADRLMDMVVKIYDNQFTVKDLKERMHAPDGMNALREQVIFITQG
                     QQTEETRNFIQNMK"
BASE COUNT         4054 a         1375 c         2129 g         2943 t
ORIGIN      
        1 cgacatgtta gaaatatttt taatagggtt tggcgtttat ctcttttatc gcatagcaat
       61 tatttttctt aagagtaaaa agactataca cacaaacata tatgaaatgt taatgcttgc
      121 tactatcttt atgatatcta catttgctta taaacatcaa aagacgcata tcttaatagc
      181 atttttagta atgtttttta tgagtaagct caaacaagtt caagggagct atgaggaatg
      241 acacaatact tagtcacaac attcaaagat tcaacaggac gtaaacatac acacataact
      301 aaagctaaga gtaatcaaag gtttacagtt gttgaggcag agagtaaaga agaagcgaaa
      361 gagaagtacg agaaacaagt taaaagggat gcagttatta aagtgggtca gttgtttgaa
      421 aatataaggg agtgtgggaa atgattaaaa aacttaaaaa tatggatggg ttcgacatct
      481 ttattgttgg aatactgtca ttattcggta taaccgcatt gctacttgtt gtcgcattgc
      541 ctatctatac agtggctagt taccaaaaca aagaagtaca tcaagggaca attacagata
      601 aatataacaa aagacaagat aaagaggaca aattctatat tgtattagat gataaacaag
      661 tcatcgaaaa ctcagactta ttcttcaaag gaaagtttga tagcgcagac atacaagcta
      721 ggttaaaagt aggtgataaa gtaaaagtta agacgattgg atatagaata cactttttaa
      781 atttatatcc ggtcttatac gaagtaaaga aggtagataa aaaatgatta agcaaatact
      841 aagattatta ttcttactag cgatgtatga gttaggtaag tatgtaactg agcaagtata
      901 tattatgatg acggctaatg atgatgtaga ggcgccgagt gattacgtct ttcgagcgga
      961 gttaagtgag tgatgtggat tactatgact attgtatttg ctatattgct attagtttgt
     1021 atcagtatta atagtgatcg tgcaagggag atacaagcgc tcagatatat gaatgattat
     1081 ctacttgatg aagtagttaa aactaaagga tacaacgggt taaaagaata caggattgaa
     1141 ttaaagcgaa tgaataacga tattaaaaag taatttatat tatcggaggt attgcattga
     1201 atgataaaga ttgagaaaca cgatatcaaa aagcttgaag aatacattca gcacatcgat
     1261 aactatcgaa gagagttgaa gatgcgagaa tatgaattac ttgaaagtca tgaaccagat
     1321 aatgcgggag ctggcaaaag taatttgccg ggtaacccga ttgaacgatg tgcaataaag
     1381 aagtttagtg ataacaggta caatacatta agaaatatag ttaacggtgt agatagattg
     1441 atagatgaaa gtgatgagga tacgcttgag ttattaaggt ttagatattg ggattgtcct
     1501 attggttgtt atgaatggga agatatagca cattactttg gtacaagtaa gacaagtata
     1561 ttacgtagaa ggaatgcact gatcgataag ttagcaaagt atattggtta tgtgtagcgg
     1621 acttttaccc tatgtaagtc cgcattaaaa cagtttatta tgttagtatc agattaatat
     1681 ttaaagttat taaatgctaa tacgacgcat gaacaagagg cgcatcacta tgtgatgtgt
     1741 ctttttattt atgaggtatg aacatgttca aactaatagt aaatacatta ctacacatca
     1801 agtatagatg agtcttgata ctacttaagt tatataaggt gaaacattat gatgactaaa
     1861 gacgaacgca tacgattcta taagtctaaa gaatggcaaa caacaagaaa aagagtgcta
     1921 gaaagagata attatgaatg tcaacaatgt aagagagacg gcaagttaac gacatatgac
     1981 aaaagcaagc gtaagtcgtt ggatgtagat catatattat cgctagaaca tcatccggag
     2041 tttgctcatg acttaaacaa tttagaaaca ctgtgtatta aatgtcacaa caaaaaagaa
     2101 aagagattta taaaaaaaga aaataaatgg aaagacgaaa aatggtaaat acccccgggt
     2161 caaaaaaatc aaaagcgatc aaaatacttg gggaacgggc aggggctcga cttcgcgata
     2221 attttaaaaa tccatgtata acccccctct tataaccatt ttaaggcagg tgatgaaatg
     2281 gagattatag ttgatgaaaa cttagtgctt aaagaaaaag aaaggctgca agtattatat
     2341 aaagacatac ctagcaataa attaaaagta gttgatggtt taattattca agcagcaagg
     2401 ctacgtgtaa tgcttgatta catgtgggaa gacataaaag aaaaaggtga ctatgattta
     2461 tttactcaat ctgaaaaggc gccaccatat gaaagggaaa gaccagtagc caaactattt
     2521 aatgctagag atgctgcata tcaaaaaata atcaaacaat tatcggattt attgcccgaa
     2581 gagaaagaag acacagaaac gccatctgat gattacctat gattagtaat aaatacgttg
     2641 atgaatatat aaatttgtgg aaacaaggaa agataatttt aaataaagaa agaattgatc
     2701 tctttaatta tctacaaaaa catatatatt cacgagatga tgtatatttt gatgaacaga
     2761 aaatcgagga ttgtatcaaa tttattgaaa aatggtattt tccaacatta ccatttcaaa
     2821 ggtttatcat agctaatata tttcttatag ataaaaatac agatgaagct ttctttacag
     2881 aatttgctat tttcatggga cgtggaggcg ggaaaaacgg tctaataagt gctattagtg
     2941 attttctttc tacgccctta cacggagtta aagaatatca catctccatt gttgctaata
     3001 gtgaagatca agcaaaaaca tcgtttgatg aaatcagaac cgttttaatg gataacaaac
     3061 gaaataagac gggtaaaacg ccaaaagctc cttatgaagt tagtaaagca aaaataataa
     3121 accgtgcaac taaatcggtt attcgatata acacatcaaa cacaaaaacc aaagacggtg
     3181 gacgtgaggg gtgtgttatt tttgatgaaa ttcattattt ctttggtcct gaaatggtaa
     3241 acgtcaaacg tggtggatta ggtaaaaaga aaaatagaag aacgttttat ataagtactg
     3301 atggttttgt tagagagggt tatatcgatg caatgaagca caaaattgca agtgtattaa
     3361 gtggcaaggt taaaaatagt agattgtttg ctttttattg taagttagac gatccaaaag
     3421 aagttgatga cagacagacg tgggaaaagg cgaacccaat gttacataaa ccgttatcag
     3481 aatacgctaa aacactgcta agcacgattg aagaagaata taacgattta ccattcaacc
     3541 gttcaaataa gcccgaattc atgactaagc gaatgaattt gcctgaagtt gaccttgaaa
     3601 aagtaatagc accatggaaa gaaatactag cgactaatag agagatacca aatttagata
     3661 atcaaatgtg tattggtggt ttagactttg caaacattcg agattttgca agtgtagggc
     3721 tattattccg aaaaaacgat gattacattt ggttaggaca ttcgtttgta agacaagggt
     3781 ttttggatga tgtcaaatta gaacctccta ttaaagaatg ggaaaaaatg ggattattga
     3841 ccattgtcga tgatgatgtc attgaaattg aatatatagt tgattggttt ttaaaggcta
     3901 gagaaaaata tgggcttgaa aaagtcatag ctgataatta tagaactgat attgtaagac
     3961 gtgcgtttga ggatgctggc ataaaacttg aagtacttag aaatccaaaa gcaatacatg
     4021 gattacttgc accacgtatc gatacaatgt ttgcgaaaca taacgtaata tatggagaca
     4081 atcctttgat gcgttggttt actaataatg ttgcagtaaa ggttaaaccc gatggtaata
     4141 aagaatatat taaaaaagat gaaaatagaa gaaaaaccga tgggttcatg gcttttgttc
     4201 acgcattata tagagcagac gatatagtag acaaagacat gtctaaagcg cttgatgcat
     4261 taatgagtat agatttctaa tagaggaggt gagacatgag tattctagaa aagatattta
     4321 aaactaggaa agatataaca tatatgcttg atttagatat gatagaagat ctatcacaac
     4381 aagcgtatgt gaaacgttta gcgattgata gttgtattga atttgttgcg cgagctgtcg
     4441 ctcaaagtca ttttaaagta ttggaaggta atagaattca aaagaatgat gtttactaca
     4501 agttaaatat aaaaccaaat actgacttat caagcgatag tttttggcaa caagttatat
     4561 ataaactaat ttatgataac gaggttttaa tcgtagtaag tgacagcaaa gaattactta
     4621 tcgcagatag cttttacaga gaagagtacg ctttgtatga tgatatattc aaagatgtaa
     4681 cggttaaaga ttatacttat caacgtactt tcacaatgca agaggtcata tatttaaagt
     4741 acaacaacaa taaagtgaca cactttgtag aaagtctatt cgaagattac gggaaaatat
     4801 tcggaagaat gataggtgca caattaaaaa actatcaaat aagagggatt ttgaaatctg
     4861 cctctagcgc atatgacgaa aagaatatag aaaaattaca agcgttcaca aataaattat
     4921 tcaatacttt taataaaaat caactagcaa tcgcgccttt gatagaaggt tttgattatg
     4981 aggaattatc taatggtggt aagaatagta acatgccttt ttctgaattg agtgagctaa
     5041 tgagagatgc aataaaaaat gttgcgttga tgattggtat acctccaggt ttgatttacg
     5101 gagaaacagc tgatttggaa aaaaacacgc ttgtatttga gaagttctgt ttaacacctt
     5161 tattaaaaaa gattcagaac gaattaaacg cgaaactcat aacacaaagc atgtatttga
     5221 aagatacaag aatagaaatt gtcggtgtga ataaaaaaga cccacttcaa tatgctgaag
     5281 caattgacaa acttgtaagt tctggttcat ttacaaggaa tgaggtgcgg attatgttag
     5341 gtgaagaacc atcagacaat cctgaattag acgaatacct gattactaaa aactacgaaa
     5401 aagctaacag tggtgaaaat gatgaaaaag aaaaagatga aaacactttg aaaggtggtg
     5461 atgaagatga aagcggagat taaaggcgtc atcgtttcca acgaagataa atgggtttac
     5521 gaaatgcttg gtatggattc gacttgtcct aaagatgttt taacacaact agaatttagt
     5581 gatgaagatg ttgatattat aattaactca aatggtggta acctagtagc tggtagtgaa
     5641 atatatacac atttaagagc tcataaaggc aaagtgaatg ttcgtatcac agcaatagca
     5701 gcaagtgcgg catcgcttat cgcaatggct ggtgaccaca tcgaaatgag tccggttgct
     5761 agaatgatga ttcacaatcc ttcaagtatt gcgcaaggag aagcgaaaga tctaaatcat
     5821 gctgcagaaa cattagaaca tgttggtcaa ataatggctg aggcatatgc ggttagagct
     5881 ggtaaaaaca aacaagaact tgtagaaatg atggctaggg aaacgtggct aaatgctgat
     5941 gaagccattg aacaaggttt tgcggatagt aaaatgtttg aaaacgacaa tatgcaaatt
     6001 gtagcaaaca atacacaagt gttatcgaaa gatgtattaa atcgtgtaac agctttggta
     6061 agtaaaacgc cagaggttaa cattgatatt gacgcaatag caaataaagt aattgaaaaa
     6121 ataaatatga aagaaaagga atcagaaatc gatgttgcag atagtaaagt atcagcaaat
     6181 ggattttcaa gattcctttt ttaatacaaa aaataggagg tcataaaatg actataaatt
     6241 tatcggaaac attcgcaaat gcgaaaaacg aatttattaa tgcagtaaac aacggtgaac
     6301 cgcaagaaag acaaaatgaa ttgtacggtg acatgattaa ccaactattt gaagaaacta
     6361 aattacaagc aaaagcagaa gctgaaagag tttctagttt acctaaatca gcacaatctt
     6421 tgagtgcaaa ccaaagaagt ttcttcatgg atatcaataa aaacgttaac tataaagaag
     6481 aaaaactttt gccagaagaa acaattgata gaatttttga agatttgacg acgaatcatc
     6541 cgttattagc tgatttaggt attaaaaacg ctggtttgcg tttgaagttc ttaaaatctg
     6601 aaacttctgg cgtagccgtt tggggtaaaa tctatggtga aattaaaggt caattagatg
     6661 ctgcgttcag tgaagaaaca gcaattcaaa ataaattgac agcgtttgtt gttttaccaa
     6721 aagatttaaa tgattttggt cctgcgtgga ttgaaagatt tgttcgtgtt caaatcgaag
     6781 aagcatttgc agtggcgctt gaaactgcgt tcttaaaagg tactggtaaa gaccaaccaa
     6841 tcggcttaaa ccgtcaagta caaaaaggtg tatcggtaac tgagggtgct tatccagaga
     6901 aagaagaaca aggtacgctt acatttgcta atccgcgcgc tacggttaat gaattgacgc
     6961 aagtgtttaa ataccactca actaacgaga aaggtaaatc agtagcggtt aaaggtaatg
     7021 taacaatggt tgttaatccg tccgatgctt ttgaggttca agcacagtat acacatttaa
     7081 atgcaaatgg cgtatatgtt actgctttac catttaattt gaatgttatc gagtctacag
     7141 tccaagaagc aggtaaggtt ttaacgtacg ttaaaggttt atatgatggt tatttagctg
     7201 gtggtattaa tgttcagaaa tttaaagaaa cacttgcgtt agatgatatg gatttataca
     7261 ctgcaaaaca atttgcttac ggcaaagcga aagataataa agttgctgct gtttggaaat
     7321 tagatttaaa aggacataag ccagctttag aaggtaccga agaaacacta taaaatttta
     7381 tgaggtgata aaatggtgaa atttaaagtt gttagagctt ttaaagacat agagcacaat
     7441 caacacaagt acaaagtagg ggagttgtat ccagctgaag ggtataacaa tcctcgtgtt
     7501 gaattgttga caaatcaaat caaaaataag tacgacaaag tttatatcgt acctttagat
     7561 aagctgacaa aacaagaatt attagaacta tgcgaatcat tacaaaaaaa agcgtctagt
     7621 tcaatggtta aaagtgaaat cgtcgactta ttgaatggtg aagacaatga cgattgatga
     7681 tttgcttgtc aaatttaaat cacttgaaag gattgaccat aattcagagg atgagtactt
     7741 aaagcagttg ttaaaaatgt cgtacgagcg tataaaaaat cagtgcggag tttttgaatt
     7801 agagaattta ataggtcaag aattgatact tatacgcgct agatatgctt atcaagattt
     7861 attagaacac ttcaacgata attacagacc tgaaataata gatttttcgt tatctctaat
     7921 ggaggtatca gaagatgaag aaagtgttta aaaaacctag aattacaact aaacgtttaa
     7981 atactcgtgt tcatttttat aagtatactg aaaataatgg tccagaagct ggagaaaaag
     8041 aagaaaaatt attatatagc tgttgggcga gtattgatgg tgtctggtta cgtgaattag
     8101 aacaagctat ctcaaacgga acccaaaatg acattaaatt gtatattcgt gatccgcaag
     8161 gtgattattt acccagtgaa gaacattatc ttgaaattga atcaagatat ttcaaaaatc
     8221 gtttgaatat aaagcaagta tcaccagatt tggataataa agactttatt atgattcgtg
     8281 gaggatatag ttcatgagtg tgaaagtgat aggtgataaa gcattagaaa gagaattaga
     8341 aaaacgtttt ggcataaaag agatggtaaa agttcaagat aaggcgttaa tagctggtgc
     8401 taaggtaatt gttgaagaag taaaaaaaca actaaagccc tcaaaagata cgggagcatt
     8461 aattaatgag gtaagtttta gtaaacctga atggataaac ggaaaacgta caattactgt
     8521 tcattggcga ggttctaaag accgttataa aatcgtacat ttaattgaat atggacacgt
     8581 tcaaaaagga acaggtaaat ttatcaaacc taaagctatg ggcggtgtta atagagcaat
     8641 aagacaaggg caaaataagt attttgagac gctaaaaagg gagttgaaaa aattgtgatt
     8701 gatattttgt acaaagttca tgaagtgatt agtcaagaca gaattattag agagcacgta
     8761 aatatcaata atattaagtt caataaatac cctaatgtaa aagatactga tgtacctttt
     8821 attgttattg acgatatcga cgacccaata cctacaactt atactgacgg agatgagtgt
     8881 gcatatagtt atattgtcca aatagatgtt tttgttaagt acaatgatga atataatgcg
     8941 agaatcataa gaaataagat atctaatcgc attcaaaagt tattatggtc tgaactaaaa
     9001 atgggaaatg tttcaaatgg aaaaccggaa tatatagaag aatttaaaac atatagaagc
     9061 tctcgcgttt acgagggcat tttttataag gaggaaaatt aaatggcagt aaaacatgca
     9121 agtgcgccaa aggcgtatat taacattact ggtttaggtt tcgctaaatt aacgaaagaa
     9181 ggcgcggaat taaaatatag tgatattaca aaaacaagag gattacaaaa aattggtgtt
     9241 gaaactggag gagacttgaa aacagcatat gctgatggtg gtccaatcga atcagggaac
     9301 acagacggag aaggtaaaat ttcgttacaa atgcatgctt tccctaaaga gattcgcaaa
     9361 atagtattca atgaagagta tgatgaagac ggtgtttata aagaaactca aggtaaacaa
     9421 aacaactatg tagcaatttg gttcagacaa gagcgtcgag acggcacatt tagaacggtt
     9481 ttattaccta aagtcatgtt tacaaatcct aaaatcgatg gagaaacggc tgagaaagat
     9541 tgggatttct caagtgaaga ggttgaaggt gaggcacttt tccctttagt tgataataaa
     9601 aagtcagtac gtaagtatat ctttgattca gctaacatga caaatcatga tggagacggt
     9661 gaaaaaggcg aagaggcttt cttaaagaaa attttaggcg aagaatatac tggaaacgtg
     9721 acagaggata gcgaagaaac tttgtaacga aaccggcttc atcggaaact gcggtaaagt
     9781 cggttaatat accagatagc attaaaacac ttaaagttgg cgacacatac gatttaaatg
     9841 ttgtagtaga gccatctaat caaagtaagt tattgaaata cacaacagat caaacgaata
     9901 ttgtatcaat caatagtgat ggtcaagtta ctgcggaagc acaaggcatt gctacggtta
     9961 aagcaacagt tggtaatatg agtgacacta taacaataaa tgtagaagca taagaggggg
    10021 caacccctct attttatttg aaaataagga gagtattata aaatggcaaa attaaaacgt
    10081 aacattattc aattagtaga agacccgaaa gcaaatgaaa ttaaattaca aacgtactta
    10141 acaccacact tcatttcatt tgaaattgta tacgaagcaa tggatttaat cgatgatatt
    10201 gaggacgaaa atagcacgat gaaaccaaga gaaatcgctg acagattgat ggatatggtt
    10261 gtaaaaattt acgataacca attcacagtt aaagacctaa aagaacgtat gcatgcacct
    10321 gatggaatga atgcacttcg tgaacaagta attttcatta ctcaaggtca gcaaactgag
    10381 gaaactagaa attttatcca gaacatgaaa taaagcctga agatttaaca tataaagcaa
    10441 tgttgaaaaa tatggatact ctcatgatgg acttaattga aaatggtaaa gacgctaacg
    10501 a
//
LOCUS       DAOBBH010000085        10356 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.085, whole genome shotgun sequence.
ACCESSION   DAOBBH010000085 DAOBBH010000000
VERSION     DAOBBH010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10356
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.085"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            68..1147
                     /locus_tag="P6L01_001542"
     CDS             68..1147
                     /locus_tag="P6L01_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HDP3030613.1"
                     /translation="MWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYAL
                     LIAITIQSIGLLILTATDILQIPAHSFPILGTIIGSFIFGIGIVLAGGCATGTWYRAG
                     EGLIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVNSDMSQTTGIPFWGLVVILT
                     IITIFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIVIGLIALLAWPM
                     SASTGRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGSREFKWR
                     LPDKITIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTMS
                     HFIFVRPMKKVQQQSAKVKQQTQIV"
     gene            1207..1431
                     /locus_tag="P6L01_001543"
     CDS             1207..1431
                     /locus_tag="P6L01_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDP3030614.1"
                     /translation="MIHELGTVGMVCPFPLIEAQKKMATLQSGDELKIDFDCTQATEA
                     IPNWAAENGYPITNYEQIDNASWTITIQKV"
     gene            1639..2889
                     /locus_tag="P6L01_001544"
     CDS             1639..2889
                     /locus_tag="P6L01_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDP3030615.1"
                     /translation="MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSM
                     AAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQ
                     MMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
                     GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFT
                     FDNIAMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVT
                     YLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKAN
                     PDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGL
                     DKIVHGEHAYFEGELNRFNKHIRY"
     gene            3072..4022
                     /locus_tag="P6L01_001545"
     CDS             3072..4022
                     /locus_tag="P6L01_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDP3030616.1"
                     /translation="MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTRIIAEHDY
                     QPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEA
                     EIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKA
                     GQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVE
                     AQKDVANVLENVEQVDAVVGATDTIALAAYKYYSDKNDVMKPHQIYGFGGDPMTQLVS
                     PSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI"
     gene            4171..5655
                     /locus_tag="P6L01_001546"
     CDS             4171..5655
                     /locus_tag="P6L01_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDP3030617.1"
                     /translation="MTEWTREERYQRIEDVDTEYFKTLKQQVDQSKFRQQFHIQPETG
                     LLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKPEGPILNPDTKYD
                     SHGVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQP
                     EGYTSHFRDPKVFKYGEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDF
                     GYMWECPDYFNLDNQDVILICPQGIEPKGNQFKNIYQSGYILGKFDIEKLTYEHENFV
                     ELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQL
                     KQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTS
                     KTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTTRSTILDTPLKQLQIFVDTSSIEIF
                     CNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK"
     gene            5652..6611
                     /locus_tag="P6L01_001547"
     CDS             5652..6611
                     /locus_tag="P6L01_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDP3030618.1"
                     /translation="MRRLFSIGEALIDFIPNVTNANLKDVQTFTKQIGGAPCNVACTV
                     QKLGQQAYMITQLGNDAFGDSIIETISSIGVDVSKVYRTNEANTALAFVSLTEAGERD
                     FSFYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGT
                     VVFDPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTG
                     NVTVVIYTKGADGAAVYLKNGINHYHSGYKVKPVDTTGAGDAFIGAVISRILATDVLN
                     LTQLFENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQAF"
     gene            complement(6799..6908)
                     /locus_tag="P6L01_001548"
                     /pseudo
     CDS             complement(6799..6908)
                     /locus_tag="P6L01_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6980..7696)
                     /locus_tag="P6L01_001549"
     CDS             complement(6980..7696)
                     /locus_tag="P6L01_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDP3030619.1"
                     /translation="MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVL
                     EQAKNMNDIGCYFLDIQLSTDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKV
                     AAMDFIFKDDPAELRTRIIDCLETAHTRLQLLSKDNSVETIELKRGSNSVYVQYDDIM
                     FFESSTKSHRLIAHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKER
                     IVYFKNKEHCYASVRNVKKI"
     gene            complement(7715..8998)
                     /locus_tag="P6L01_001550"
     CDS             complement(7715..8998)
                     /locus_tag="P6L01_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDP3030620.1"
                     /translation="METINNLAMATFQLVILFTVAKFISFVKFNLRDYFIIVGIIIPT
                     MFLYYFYGSRAVLIPTFSSIIFLFFKLKYYAIVTILVTMIIMYLSNFATVGLFLTLRK
                     YTTDPAILLPLYILSFSSVSLLATYLVRISLKKFKKSYLSLNKTYMIIISFVLFATFA
                     FFYIYSTNTSSNGDSLIPYALVFIGLIIFISVVILIMSLFTLKEMKYKRNQEEIETYY
                     EYTLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMIGLRAYFNKNIVPMKDNLQMNA
                     IKLNGIENLKVREIKGLITAKILRAQEMNIPISIEIPDEVSSINLNMIDLSRSIGIIL
                     DNAIEASTEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGL
                     GLSTLKEIADNADNVLLDTIIENGFFIQKVEIINN"
     gene            complement(9028..9171)
                     /locus_tag="P6L01_001551"
     CDS             complement(9028..9171)
                     /locus_tag="P6L01_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDP3030621.1"
                     /translation="MNTLVNMFFDFIIKLAKAIGIVGGVNACSSLFDEPKVPAELTNL
                     YDK"
     gene            complement(9174..9737)
                     /locus_tag="P6L01_001552"
     CDS             complement(9174..9737)
                     /locus_tag="P6L01_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator ArgB-like protein"
                     /protein_id="HDP3030622.1"
                     /translation="MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQIIVGNFFKIL
                     VTYSISIFLSVFLFTLVTHLSYMLIRYNAHGAHAKSSILCYIQSILTFVFVPYFLINI
                     DINFTYLLALSIIGLISVVIYAPAATKKQPIPIKLVKRKKYLSIIMYLLVLILSLIIH
                     PFYAQFMLLGILVESITLLPIFFPKED"
     gene            9973..10107
                     /locus_tag="P6L01_001553"
     CDS             9973..10107
                     /locus_tag="P6L01_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lysin family phenol-soluble modulin"
                     /protein_id="HDP3030623.1"
                     /translation="MSCLILRIFILIKEGVISMAQDIISTIGDLVKWIIDTVNKFTKK
                     "
BASE COUNT         3656 a         1412 c         1829 g         3459 t
ORIGIN      
        1 ggttgaaaac aagaaaatca tcacctataa tacatactaa acttatcgga aatgaggggt
       61 taggattatg tggatgattt ttagtggctt aattgtcggg ggactcttag gttttgtcat
      121 gcaaagaaca agattttgtt taacaggtgg ctttcgagat atgtatgtgc aaaagaataa
      181 taagatgttc tatgcattat taatcgctat tactattcaa agtataggat tattgatttt
      241 gacggcaaca gatattttac aaattcctgc acatagtttt ccaatattgg gaacaattat
      301 aggttctttt atttttggaa ttggaatagt attggctgga gggtgtgcaa caggtacttg
      361 gtatcgcgct ggtgaagggc taattggtag ttggattgca ttagtattat atgctgttac
      421 tgcagcaatc actaaaacag ggattttaaa gccagtaatg gataaaatta atcaaccaac
      481 gaatgtaaat agtgatatgt ctcaaacaac tggcattccg ttttggggat tagtcgttat
      541 attaactata atcaccattt ttctagttgt aagaacactc aataataaga aagttcgagt
      601 tgctgttcca aaactaaagc aacgttatac aggtattaga tattaccttt tcgaaaaacg
      661 ataccatcca tttattgcag caattgtaat tggacttatc gcactcttag cttggccaat
      721 gagtgcatca actggaagaa atgacggttt aggtataaca acgccttcag caaatttagt
      781 acactttttg attacaggtg aaactaaatt tattgattgg ggtgtctttt tagttctagg
      841 aattttcatt ggttcatata ttgcagctag aggatcaaga gaatttaaat ggcgattgcc
      901 agacaagatt acaatacgaa acagtgccat tggtggcata tgtatgggat ttggtgcgtc
      961 agttgctggt ggttgttcta tcggtaacgg tttggttgaa acggcaacga tgacttggca
     1021 aggatggatt gcgctagcat cgatgatagt tggtgtatgg acaatgagtc attttatctt
     1081 tgttcgtcca atgaaaaaag tacaacaaca atccgcaaag gttaaacagc aaacgcaaat
     1141 agtatagaag attattatgc aaatgatgtt gatcaaataa aagtgattgg aaaaggagaa
     1201 ataattatga tacacgaatt aggtacagta ggaatggtat gtccatttcc gttaattgaa
     1261 gcgcaaaaga aaatggcaac attgcaatct ggagatgaat taaaaattga ttttgattgc
     1321 acgcaagcga cggaagccat tccaaattgg gctgcagaaa atggttatcc tataacaaac
     1381 tatgaacaaa ttgataatgc ttcatggaca attacaattc aaaaagttta acgttatcat
     1441 tttaacaata aaatagatat tagattctat ggctacttcc gctaatttaa aagtgagtaa
     1501 gtagtctttt ttgttttagt tcatgaaatc atttttatat agtgtggcat attttatttc
     1561 aaaaaatgta ataaaactta acgcattttt gctttttata aattgtcaga ttattatgaa
     1621 aacaagggag tggtaagtat gaatcttaac gatacgatat ttatgttttt gtgtacatta
     1681 ttagtttggt taatgacacc aggattaagt ttattttatg gtgggttagt tcaatctaaa
     1741 aatgcgctta atactgtcat gcaaagtatg gcagcaattg tgcttgttac atttgtatgg
     1801 ataacagttg gttttacaat tagttttggg aatgggaatt tatggttcgg aaattgggaa
     1861 tatacttttc ttaatcatgt aggttttgcg actcaagaag atattagccc acatattcct
     1921 ttcgctttgt ttatgttatt tcaaatgatg ttttgtacga ttgcaatttc tattttatct
     1981 ggttcaatcg ctgagaaaat gaagtttatt ccttatttat tattcgtagt aatatggact
     2041 gctcttgtat acagtccagt agcacattgg gtttggggcg gtggttggat taataaactc
     2101 ggtgtattag atttcgctgg aggtacggtt gttcatatta catcaggtgt ttctggttta
     2161 gtattagcta ttatgattgg aaaaggaaac aaacattctg aatcaacacc acataatctt
     2221 atcattacgt tgattggcgg tatattcgtg tggattggtt ggtatggatt taatgtaggt
     2281 agtgctttta catttgataa tattgcgatg cttgcattta caaatactgt catttcagcc
     2341 agtgcaggtg ctataggttg gttaatttta gaatatattt ttaaaaagac gacaagttta
     2401 cttggacttt tactcggtgc attagcagga ttagttgtta ttactcctgc agcaggatat
     2461 gtaacatatc ttagtgcaac aataatggct ttaataggag gtatctgttg ttatattgtc
     2521 attaattaca tcaaggtaaa actaaaatat catgatgcat tagatgcatt tggtattcat
     2581 ggtgttggtg gtattattgg tgctgtttta acagcagttt tccaaagtaa aaaagccaat
     2641 cctgacattg agaatggctt tatttatact ggtgacatac atattatact tgtacaaata
     2701 ttatgtgtaa cagcagttgt aatttttagt atcgtcatga cgtttattat tgcgaaagta
     2761 attaaattaa ttacaccatt atctgttacg gaacaagaaa cgaatatagg attagacaag
     2821 attgttcacg gtgaacatgc ttactttgaa ggtgagctaa atagattcaa taaacatatt
     2881 cgatattaga atatatttac atagaatatt cattgtcctg acatttaact aaaggttgat
     2941 gttgggacat tttgttatac aaaagtttta ttttgaaatc tttttatgaa agaagcagaa
     3001 atattattta aagcggttac acatatgcta aaataaggct aagtgtcaca aataatgata
     3061 ggtgaataag tatgaaaaat atatctgata ttgccaaatt ggcaggcgtt tcaaaaagca
     3121 cagtatctag atttttaaat aatggatctg tcagtaaaaa aacaagtgaa aaattaacaa
     3181 gaattatagc agaacatgac tatcaaccga atcaatttgc tcaaagttta agagcgagac
     3241 aaacacacct tattggtgcc attataccta gaatgaattc atatgcagta gatgaaacaa
     3301 tcaaaggatt ggcaaaacaa tgccaaaaat atgaatcaca attaatttta aattacacag
     3361 gtttaaatat cgaagcagaa atacaagcgc ttgaaacatt agcacgcagt aaagtagatg
     3421 gtattgtttt aatggctaca gacataacag agagacatat tgaagtcatt aataaaatga
     3481 atgtaccaat cgttattgtt ggtcaacaac atgaacaact tcatagtatt gtgcatgatg
     3541 attataaagc aggtcaaatt ataggcgaat ggattggtca acagggatat caacaagttg
     3601 aagtgtttag tgtaagtgaa aaagatattg cagttggtat acatagaaaa cgtggtttac
     3661 ttgaccagtt agctaaatac caaattaaac ctaatataca tgaaacaaat tttacttatg
     3721 tggaagcaca aaaagatgtt gcaaatgttt tggaaaatgt ggagcaagta gatgcggttg
     3781 ttggagcaac tgatacgatt gcattagctg cctataaata ttattctgat aaaaatgatg
     3841 ttatgaaacc acatcaaata tatggttttg gtggtgaccc aatgacacaa ttagtgtctc
     3901 catcgataaa aacaattcat tataattatt ttgaagctgg ccaatgcgcg atggaagaga
     3961 tacaacagat gcttaaaaag caagatatgc catatagcgt cacagtagat gttaatattt
     4021 agacgctgta ttttttaaaa caaatgtgga accgatacca tataactata aatggatagg
     4081 ttaaaagtta aagaacgtag gtaaaatttg ctataataga atataaattg ttaacagcat
     4141 aaattataaa aggaggactg ggtaaatatt atgaccgaat ggactagaga agaacgttat
     4201 caacgaatcg aggacgttga tactgagtat tttaaaacat taaaacaaca agttgatcaa
     4261 tcaaaatttc gtcaacaatt tcatatacaa ccagaaacag gtttattaaa tgatcccaac
     4321 ggacttattt tttataaagg gaagtattat gtttcacatc aatggttccc attaggcgca
     4381 gtacatggct taaagtattg gtataactac acgagtgatg acttaataaa ctttaaacct
     4441 gaagggccaa tattaaatcc agatactaaa tatgacagcc atggtgttta tagcggtagc
     4501 gcttttgaat ataacgggca tttatattat atgtacacag gaaatcatcg agataatcat
     4561 tggcaacgac atgcgagtca gatgatcgca cgattgaaag aagacggttc agttgaaaag
     4621 tttccaaagc cagtaattag ccagcaacca gaaggatata caagtcattt tagagatcct
     4681 aaagttttta aatatggtga gaaatattat gcaatcattg gcgcacaaaa taatgatcag
     4741 caaggtcgat tattacttta taatactgaa gatataatta attggcatta tttaggtgaa
     4801 ataaatacag agttagatga ttttggatat atgtgggaat gcccagatta ctttaattta
     4861 gataatcaag atgtcatact tatttgtcca caaggtattg aaccaaaagg caatcagttc
     4921 aaaaatattt atcaaagtgg ttatatactt ggaaagtttg atattgaaaa gttaacatat
     4981 gaacatgaaa attttgtcga gcttgataat ggctttgatt tctatgcacc tcaaacattt
     5041 ttagatgaaa aaggccgacg agtactaatt ggatggatgg ggttaccgga aatcgaatat
     5101 cctactgata atgaaggatg ggcccattgc ttaacaattc ctcgtgtatt aaatgtagaa
     5161 aatggtcaac ttaagcaacg tccatatcca gcgttggaaa aattacgtca caataaagag
     5221 acagcattag gctacgcaaa taaatttact cgaaaattac atccgtatga aggtaaacag
     5281 tatgaattaa tcatagatat tttggataat gatgctaccg aagtgtactt tgaattacgt
     5341 acatctaaga cttcttcaac attaattgct tataacaagc gtgaaaataa aataacatta
     5401 gatcgcagcg acagtggttt attgccgaca aatgttgaag gtacgacgcg tagtacgata
     5461 ttagacacgc cattaaagca attacaaatt tttgttgata catctagtat agaaattttc
     5521 tgtaatgatg gggaacgtgt tttgacatct cgaattttcc cgacagaaga cgcactaggt
     5581 attaaaacat caacagaatc agggcaagta tatttacaat ttactaaata tgatttaaag
     5641 gatgaacata aatgagacgt ctattttcaa taggagaagc gttaattgat tttattccaa
     5701 atgttacaaa tgcgaattta aaagatgttc aaacatttac gaagcaaatt ggtggcgcgc
     5761 catgcaatgt agcttgtaca gttcaaaagt taggacaaca agcgtatatg attacacaat
     5821 taggtaatga tgcatttgga gatagcatta ttgaaacgat atcaagtatt ggtgtagatg
     5881 tatcaaaagt gtatcgcacg aatgaagcaa acactgcgtt agcttttgtg agtttgactg
     5941 aagcgggcga gcgagatttt tctttttatc gtaagccctc tgctgatatg ttatttgaac
     6001 caagttttgt gaatgatatt gatgtgaatg aaaatgatgt agtacatttt tgttctgttg
     6061 atttggtcga tagtccgatg agagatgcac attatcaatt gattaccaaa acattaaatg
     6121 caaatggtac tgtcgtattt gatcctaatg taagattgcc attatgggat aacgctgaag
     6181 atttaagaca aactattcat acctttttgc cattggcgca tattgtaaaa gtttcagatg
     6241 aagaattaga atttattaca ggtattcatg atgaaaatga agccattcaa tcattattta
     6301 caggtaatgt tactgtagtc atttatacga agggagcaga tggtgctgcc gtatatttga
     6361 aaaatggtat taatcattat catagtggct ataaggtaaa acctgttgat acaactgggg
     6421 caggggatgc ttttattggt gcagttatta gcaggatttt agcaaccgat gtgttaaatt
     6481 taactcagtt atttgaaaat gaaggtgagg aaattttagc gtttagcaat cgcgttgctg
     6541 caatagtgac aacgaagtat ggtgccatta atagtttgcc aacattacaa gaggttgaac
     6601 aagcatttta aataattcat ttataaaaca gtgtaggaaa caagttgaat cttgtgctac
     6661 actgtttttt aaaacaaaat aagattcacg gggtaggaaa ttgtagcatt ttatttatgg
     6721 aaactgtcaa tttcaaattt agtatatttt gttaaaaatt gcgccatagg attgtagagt
     6781 gaaatagaaa ttgataaatt atgtagtaat aggaataggt atacaaagaa acgttacatc
     6841 attttttaat tcacgcgtca tatttaattt tgttattaaa caattaaaaa gccagctata
     6901 cagtgcattt gctagttatc ttgttaaaat ccaacaagat ttacaattga atacgccgtt
     6961 aactgacttt attatcttat tatatttttt taacgtttct caccgatgca tagcagtgtt
     7021 ctttattttt aaaatagaca attcgctctt tcgaatctat agattcaata ttatggcgat
     7081 tgacgacaaa gctattatga catctaaaga aacgatcatc taattgactc agttctttta
     7141 aattaccata aaattcaatt tgacggttat ctaaatgggc aatgagtctg tgagattttg
     7201 ttgatgattc aaaaaacata atatcatcat attgaacata cactgaatta ctgccacgtt
     7261 ttaattcaat cgtttcaacg ctattatctt tagacaacaa ttgtaagcgt gtatgtgcag
     7321 tttctaaaca atctataatt cgagttctta attctgctgg atcatcttta aaaataaaat
     7381 ccatcgctgc aactttgtag acaaatgtta aataggtaag ttcactgtga ctcgtaacga
     7441 aaataatgtt accaactggg tcatgcttac gaatttcact gcctaatttg ataccattaa
     7501 tatcagttga aagttgaata tctaaaaagt aacagcctat gtcattcata tttttagctt
     7561 gctcaagcac ctcataagga ttatcagttg cgagggcaat ttccataggc ttttcttcta
     7621 tcattatata atttttaata atggtaacca tgttttctct ttgttttgga tcgtcttcgc
     7681 aaatgaaaat tttcatacat tcacatcctt atggctagtt gttaataatt tcaacttttt
     7741 gaataaagaa accattttcg ataattgtat ctaataagac attgtctgca ttatcagcaa
     7801 tttcttttaa agttgataga cctaaaccac gaccttcacc tttagtagaa aaactttctt
     7861 ggaacaattc atgaatgcgt ggtatatcat cagcgcattt attcataaca ataaacgtta
     7921 ctgaattttc actttcaata aatgcaacgc gaatgatagg gtcatcaatt tcagttgatg
     7981 cctcaattgc attatcaaga ataataccaa tactgcgact taaatcgatc atattcaagt
     8041 taatgctact tacttcatcg ggtatttcga tactaattgg aatattcatt tcttgtgcac
     8101 gtaaaatttt cgcagtaatt aagcctttta tctcacgtac tttaagattc tcgataccat
     8161 ttaatttaat agcattcatc tgcaaattat ctttcatagg tacaatattt ttattgaaat
     8221 aagcgcgtag accaatcatg tcatcttcgc gaatgtattc tgaaagtgtc gttaagatat
     8281 tgacataatc atgacggaac ttgcgcattt cattattaat tgcttcaatc tttaatgtgt
     8341 attcgtaata ggtctcaatt tcttcttgat ttcgtttgta tttcatctct ttaagggtga
     8401 aaagcgacat tataagtatt acaacagaga taaaaattat taatcctata aatacaagtg
     8461 catatggaat taagctatca ccgtttgaac ttgtatttgt agaataaata taaaaaaatg
     8521 caaatgttgc aaataatacg aatgaaatta taatcatata agttttattt aaagataagt
     8581 atgatttttt aaatttcttt agagatattc ttaccaaata tgtcgctaat aatgaaactg
     8641 atgaaaaaga aagtatatat aatggtaaga gtattgcagg atcagtagtg tattttctta
     8701 aagttaaaaa caaacctact gttgcaaaat tactcaaata cattatgatc atggtcacta
     8761 aaattgttac tattgcatag tactttaatt taaagaataa aaatatgata gatgaaaaag
     8821 ttggaatcaa tacggctcta cttccataga agtaatacaa aaacatggtt ggaattataa
     8881 ttccaactat aataaaataa tcccttaaat taaatttaac aaaggaaatg aatttggcta
     8941 ctgtaaaaag tattactaat tgaaaagtgg ccatagctaa gttgtttatt gtttccatat
     9001 gctacacact ttctaaaata acttttacta tttgtcgtat aaattcgtta attcagcggg
     9061 tactttaggt tcatcaaata aactgctgca agcgtttacg ccaccgacaa ttccgattgc
     9121 tttagctaat ttaattataa aatcaaaaaa catattaaca agtgtattca tgattaatcc
     9181 tccttaggga aaaaaatagg taataatgta atagattcca ctaaaattcc taataacata
     9241 aattgagcat aaaatggatg tattattaag gatagaatca aaactaataa atacataatt
     9301 atagataaat actttttacg ttttactaat tttattggta taggttgctt tttcgttgct
     9361 gcaggtgcat aaataacaac ggaaatcaat cctataatag ataaggccag taaataagta
     9421 aaattaatgt ctatattaat tagaaaataa ggtacaaata caaatgttaa aatactttgt
     9481 atgtaacaaa gtatcgaaga ctttgcatga gcaccatgtg cattatagcg tattaacata
     9541 taggataagt gtgtaactaa ggtaaacaaa aatacactta agaatataga aatactatat
     9601 gttacaagta ttttaaaaaa attaccaact ataatttgca tccctaaacg tacttgcaaa
     9661 aattgaatat gatctaagtt atttctcttt tgaagatacg tggcaaactg gtcaattttg
     9721 ttatcaaaat aattcaattt tacaccactc tcctcactgt tattatacga tttagtacaa
     9781 tcttttatca ttatattgcc taactgtagg aaataaatac ttaactgtta aatgtaattt
     9841 gtatttaata ttttaacata aaaaaattta cagttaagaa taaaaaacga ctagttaaga
     9901 aaaattggaa aataaatgct tttagcatgt tttaatataa ctagatcaca gagatgtgat
     9961 ggaaaatagt tgatgagttg tttaatttta agaattttta tcttaattaa ggaaggagtg
    10021 atttcaatgg cacaagatat catttcaaca atcggtgact tagtaaaatg gattatcgac
    10081 acagtgaaca aattcactaa aaaataagat gaataattaa ttactttcat tgtaaatttg
    10141 ttatcttcgt atagtactaa aagtatgagt tattaagcca tcccaactta ataaccatgt
    10201 aaaattagca agtgagtaac atttgctagt agagttagtt tccttggact cagtgctatg
    10261 tatttttctt aattatcatt acagataatt atttctagca tgtaagctat cgtaaacaac
    10321 atcgatttat cattatttga taaataaaat tttttt
//
LOCUS       DAOBBH010000086        10351 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.086, whole genome shotgun sequence.
ACCESSION   DAOBBH010000086 DAOBBH010000000
VERSION     DAOBBH010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10351
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.086"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            252..1481
                     /locus_tag="P6L01_001554"
     CDS             252..1481
                     /locus_tag="P6L01_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDP3030624.1"
                     /translation="MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAW
                     FFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFASLTNVKMMDMAVAALFPILLIVP
                     LFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
                     NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEE
                     KEDIIYSLKNNEVERQPFFSFLGDSVLAAGKLVLIIIAFVISFVALADLFDRFINLIT
                     GLIAGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGE
                     ISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLS
                     GILVSLLTAAFVGLFAW"
     gene            1736..1828
                     /locus_tag="P6L01_001555"
     CDS             1736..1828
                     /locus_tag="P6L01_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030625.1"
                     /translation="MFCYYKQHKGDNFSIEEVKNIIADNEMKVN"
     gene            2006..2839
                     /locus_tag="P6L01_001556"
     CDS             2006..2839
                     /locus_tag="P6L01_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDP3030626.1"
                     /translation="MNKTVKDLILVVLGSFIFAAGVNAFIISGNLGEGGVTGLAIILY
                     YAFHISPAITNFLVNAVLIAIGYKFLSKRSMYLTILVTILISIFLSLTESWQVETGNS
                     IVNAIFGGVSVGLGIGVIILAGGTTAGTTILARIATKYLDVSTPYALLFFDMIVVAIS
                     LTVIPLDKVLVTVISLYIGTKVMEYVIEGLNTKKAMTIISTNPDKLAKAIDEQIGRGL
                     TILNGHGYYTREEKDVLYVVISKTQVSKAKRLIKQIDKDAFLVIHDVRDVYGNGFLAD
                     E"
     gene            3121..3918
                     /locus_tag="P6L01_001557"
     CDS             3121..3918
                     /locus_tag="P6L01_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3030627.1"
                     /translation="MNRLHGQQVKIGYGDNTIINKLDVEIPDGKVTSIIGPNGCGKST
                     LLKALSRLLAVKEGEVFLDGENIHTQSTKEIAKKIAILPQSPEVADGLTVGELVSYGR
                     FPHQKGFGRLTAEDKKEIDWAMEVTGTDTFRHRSINDLSGGQRQRVWIAMALAQRTDI
                     IFLDEPTTYLDICHQLEILELVQKLNQEQGCTIVMVLHDINQAIRFSDHLIAMKEGDI
                     IATGSTEDVLTQEILEKVFNIDVVLSKDPKTGKPLLVTYDLCRRAYS"
     gene            3954..4958
                     /locus_tag="P6L01_001558"
     CDS             3954..4958
                     /locus_tag="P6L01_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDP3030628.1"
                     /translation="MTNRENPTPLKFLSYIIGLSMILLITLFISTLIGDAKIQASTII
                     EAIFNYNPSNQQQNIINEIRIPRNIAAVIVGMALAVSGAIIQGVTRNGLADPALIGLN
                     SGASFALALTYAVLPNTSFLILMFAGFLGAILGGAIVLMIGRSRRDGFNPMRIILAGA
                     AVSAMLTALSQGIALAFRLNQTVTFWTAGGVSGTTWSHLKWAIPLIGIALFIILTISK
                     QLTILNLGESLAKGLGQNVTMIRGICLIIAMILAGIAVAIAGQVAFVGLMVPHIARFL
                     IGTDYAKILPLTALLGGILVLVADVIARYLGEAPVGAIISFIGVPYFLYLVKKGGRSI
                     "
     gene            4955..5971
                     /locus_tag="P6L01_001559"
     CDS             4955..5971
                     /locus_tag="P6L01_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDP3030629.1"
                     /translation="MISSNNKRRQLIALAVFSILLFLGCTWSITSGEYNIPVERFFKT
                     LIGQGDAIDELILLDFRLPRMMITILAGAALSISGAIVQSVTKNPIAEPGILGINAGG
                     GFAIALFIAIGKINADNFVYVLPLISILGGITTALIIFIFSFNKNEGVTPASMVLIGV
                     GLQTALYGGSITIMSKFDDKQSDFIAAWFAGNIWGDEWPFVIAFLPWVLIIIPYLLFK
                     SNTLNIIHTGDNIARGLGVRLSRERLILFFIAVMLSSAAVAVAGSISFIGLMGPHIAK
                     RIVGPRHQLFLPIAILVGACLLVIADTIGKIVLQPGGVPAGIVVAIIGAPYFLYLMYK
                     TKNV"
     gene            6205..7173
                     /gene="dhaK"
                     /locus_tag="P6L01_001560"
     CDS             6205..7173
                     /gene="dhaK"
                     /locus_tag="P6L01_001560"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDP3030630.1"
                     /translation="MMKKLINKKETFLTDMLEGLLIAHPELDLIANTVIVKKAKKEHG
                     VAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPTPDKILEAIKAVDTGDGVLLVV
                     KNYAGDVMNFEMAQELAEMEGINVQTVIVRDDIAVTNEVQRRGVAGTVFVHKLAGYLA
                     EKGYSLTEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKG
                     IHREEVKDIDHIVGTLLDELYKEVTANDVILMVNGMGGTPLSELNIVTKYIQQNLAAR
                     TVNVAKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYFK"
     gene            7215..7799
                     /gene="dhaL"
                     /locus_tag="P6L01_001561"
     CDS             7215..7799
                     /gene="dhaL"
                     /locus_tag="P6L01_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDP3030631.1"
                     /translation="MKVNDMKARLLNLEETFKKHESELTELDRAIGDGDHGVNMVRGF
                     SSLKDKLDDSSMQSLFKSTGMALMSNVGGASGPLYGFSFVKMSAVTKDDMDNQDFITL
                     IQAFAEAVESRGKVTLNEKTMYDVVARAAEKLENGETLTFNDLQQLADNTKDMVATKG
                     RAAYFGEESKGYIDPGAQSMVYILNALIGDEDNA"
     gene            7792..8154
                     /locus_tag="P6L01_001562"
     CDS             7792..8154
                     /locus_tag="P6L01_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDP3030632.1"
                     /translation="MPKIILVSHSKEIASGTKSLLKQMAGDVDIIPIGGLPDGSIGTS
                     FDIIQEVLTKLEDDALCFYDIGSSEMNVDMAIEMYDGNHRVLKVDAPIVEGSFIAAVK
                     LSIGGSIDDALAEIKQSF"
     gene            8270..8767
                     /locus_tag="P6L01_001563"
     CDS             8270..8767
                     /locus_tag="P6L01_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030633.1"
                     /translation="MQHLIKKHVLNGEFDLVRQLMSETDFMEFEEAYISSAHEVESMM
                     FYTCILDMIKYEESSEMHDLAFLLLVYPLSEYEGALDSAYYHADASIKLTDGKEVKSL
                     LQMLLLHAIPTPVISDKKAFDIAKQILKLDPNNNVARNVLKDTAKRMDNVVVDINELH
                     QRNAR"
     gene            9224..10291
                     /locus_tag="P6L01_001564"
     CDS             9224..10291
                     /locus_tag="P6L01_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030634.1"
                     /translation="MMMNKEATKIGFAYVGIVVGAGFSTGQEVMQFFTKYGLWAYLGV
                     IISGFILAFIGRQVAKIGTAFEATNHESTLQYVFGEKFSKVFDYILIFFLFGIAVTMI
                     AGAGATFEESYNIPTWLGALIMTLAIYITLLLDFNKIVRALGIVTPFLIVLVVLIAGV
                     YLFKGHVSLAEVNQVVPEASIWKGIWFGTIYGGLAFSVGFSTIVAIGGDTEKRTVSGA
                     GAMYGGIIYTVLLALINFALQSEYPTIKNASIPTLTLANNIHPLIATVLSVIMLAVMY
                     NTILGLMYSFAARFTEPYSKNYHIFIIIMMVAGYLLSFVGFAELINKLYTIMGYVGLF
                     IVVAVIIKYFKRKNADKKHIA"
BASE COUNT         3450 a         1417 c         2001 g         3483 t
ORIGIN      
        1 tctattttaa atcatttgag aagttacgac gtaataattc ttaaattaat aaaatcgata
       61 ttttaagaaa aaaatgctca tggtataata caagttataa gcaaacatac atatattaaa
      121 tactgtagcc acgagtcata attcttcata ttttacatag caatttaact gattttagag
      181 tccacggtac agaagtttga tatttcaatg tttctaaatt tttaaaaaat taaatcatag
      241 gtgggtgcta aatgttttta ttaatcaaca ttattggtct aattgtattt cttggtattg
      301 cggtattatt ttcaagagat cgcaaaaata tccaatggca atcaattggg atcttagttg
      361 ttttaaacct gtttttagca tggttcttta tttattttga ttggggtcaa aaagcagtaa
      421 gaggagcagc caatggtatc gcttgggtag ttcagtcagc gcatgctggt acaggttttg
      481 catttgcaag tttgacaaat gttaaaatga tggatatggc tgttgcagcc ttattcccaa
      541 tattattaat agtgccatta tttgatatct taatgtactt taatatttta ccgaaaatta
      601 ttggaggtat tggttggtta ctagctaaag taacaagaca acctaaattc gagtcattct
      661 ttgggataga aatgatgttc ttaggaaata ctgaagcatt agccgtatca agtgagcaac
      721 taaaacgtat gaatgaaatg cgtgtattaa caatcgcaat gatgtcaatg agctctgtat
      781 ccggagctat tgtaggtgcg tatgtacaaa tggtaccagg agaactggta ctaacggcaa
      841 ttccactaaa tatcgttaac gcgattattg tgtcatgctt gttgaatcca gtaagtgttg
      901 aagagaaaga agatattatt tacagtctta aaaacaatga agttgaacgt caaccattct
      961 tctcattcct tggagattct gtattagcag caggtaaatt agtattaatc atcatcgcat
     1021 ttgttattag ttttgtagcg ttagctgatc tatttgatcg ttttatcaat ttgattacag
     1081 gattgatagc aggatggata ggcataaaag gtagtttcgg tttaaaccaa attttaggtg
     1141 tgtttatgta tccatttgcg ctattactcg gtttacctta tgatgaagcg tggttggtag
     1201 cacaacaaat ggctaagaaa attgttacaa atgaatttgt tgttatgggt gaaatttcta
     1261 aagatattgc atcttataca ccacaccatc gtgcggttat tacaacattc ttaatttcat
     1321 ttgcaaactt ctcaacgatt ggtatgatta tcggtacatt gaaaggcatt gttgataaaa
     1381 agacatcaga ctttgtatct aaatatgtac ctatgatgct attatcaggt atcctagttt
     1441 cattattaac agcagctttc gttggtttat ttgcatggta atatgtcgaa gagtgactat
     1501 gataatacat tttaactaat aaatatgtcc aggcatgtcg tctattgata taggtgagat
     1561 gcttggactt ttttattatt gatataaagg tatttaaata tttttaaagt taccgaaatt
     1621 gaagcattat aaaaaccaag tgcacatggt aatacacttg gcttttatgg gaaatgaata
     1681 ttattgtaca tatgacagta aggactaggt acagtcatag tacttcgagc aaaatttgtt
     1741 ttgttattat aaacaacaca aaggagataa cttctctatt gaagaagtta aaaacattat
     1801 agcagacaat gaaatgaaag taaattaaaa attcagaata tttttaatta ttatattgtg
     1861 agtgatattt attagggaaa gctattcttc atataaatta gttaaatagt aaatctttgt
     1921 tagaactctt tccatagagt tgtgacagca tttttgttgt gctactattt ttatagtcta
     1981 ataaatatat aaaggggatg gtttcgtgaa taaaacggtt aaagatttaa tactagttgt
     2041 cttaggttca tttatctttg ctgcaggtgt aaatgcattt attatttctg gtaacttagg
     2101 tgaaggcggg gttacaggtt tagcaattat tttatattat gcgtttcata tttcaccagc
     2161 catcactaac ttcttggtca acgcagtatt gattgccata ggttataaat ttttgagtaa
     2221 gagaagtatg tacttaacta ttcttgtaac aattcttatt tcaatatttt tgagtttaac
     2281 agaatcatgg caagtagaaa ctggaaacag cattgtgaat gccatttttg gtggtgtaag
     2341 cgttggacta ggaatcggag taattatcct tgcaggcggt acaacagcag gtacaacaat
     2401 tttggcgaga attgcaacga aatacctcga tgtaagcacg ccatatgctt tgcttttctt
     2461 cgatatgatc gttgttgcaa tttcacttac agttattcca cttgataaag tattagtaac
     2521 agtaatatca ctttatatag gaacaaaagt gatggaatat gtcatagaag gtttaaacac
     2581 taaaaaagct atgacgatta tttcaactaa tcccgacaaa cttgccaaag caatagacga
     2641 gcaaattgga agaggtttaa ccattttaaa cggacatggc tattatacgc gtgaagaaaa
     2701 agatgtctta tacgttgtta tttctaaaac acaagtttca aaagcaaagc gattaattaa
     2761 acaaatcgat aaagatgcat tcctcgtaat tcatgatgta agagatgtct atggtaatgg
     2821 ctttcttgca gatgaataaa taaatggtat gagcacacat acttaaatag aagtccacgg
     2881 acaagttttt gaactatgaa gacttatctg tgggcgtttt ttattttata aaagtaatat
     2941 acaagacatg acaaatcgag ctatccaatt taaaaagtaa tgttagtcaa taagattgaa
     3001 aaatgttata atgatgttca tgataatcat tatcaattgg gatgcctttg aaaattgata
     3061 atttaaaaat agaaattatt ttttataaac agaaagaatt ttattgaaag tagggaaatt
     3121 atgaatcgtt tgcatggaca acaagttaaa attggttacg gggataacac gattataaat
     3181 aaattagatg ttgaaatacc agatggcaaa gtgacgtcaa tcattggtcc taacggctgc
     3241 gggaaatcta ctttgctaaa ggcattgtca cgtttattgg cagttaaaga aggcgaagta
     3301 tttttagatg gtgaaaatat tcatacacaa tctacgaaag agattgcaaa aaaaatagcc
     3361 attttacctc aatcacctga agtagcagat ggcttaactg ttggggaatt agtttcatat
     3421 ggtcgttttc cacatcaaaa aggatttggt agattaactg ctgaggataa gaaagaaatt
     3481 gattgggcaa tggaagttac aggaactgat acattccgac accgttcaat caatgattta
     3541 agtggtggtc aaagacaacg tgtttggatt gcaatggcat tagcacaaag aactgatatt
     3601 atctttttag acgaaccaac aacatattta gatatctgtc atcaattaga aatactagaa
     3661 ttagttcaga agctaaatca ggaacaaggt tgtacaattg tcatggttct tcatgatatc
     3721 aaccaagcga ttcgtttctc agatcatctt attgcgatga aagaagggga tatcatcgct
     3781 acaggttcaa cagaagacgt attaacacag gaaatattag aaaaagtttt taatattgat
     3841 gttgttttaa gtaaagatcc taaaactgga aaacctttac tggtaactta tgacttatgt
     3901 cgcagagctt attcttaatt aagtaagtta atatgataaa aaggacaatt aacatgacaa
     3961 atagagagaa cccaacgcca ttgaagtttt tatcctatat tataggttta agtatgatac
     4021 tactaatcac actatttatt tctacattaa taggtgacgc caaaattcaa gcctctacaa
     4081 ttatagaggc tatttttaat tataatccta gcaatcaaca gcaaaacatc atcaatgaga
     4141 ttaggattcc cagaaatata gcagcagtaa ttgtaggtat ggcgcttgca gtttctggtg
     4201 cgattataca aggtgttact cgtaatggtc ttgctgatcc ggcgctcata ggtttaaatt
     4261 caggtgcttc atttgcttta gcattaacat atgcagtttt accaaacact tcatttttaa
     4321 tattgatgtt tgctggattt ttaggtgcta ttctaggagg tgctattgta ttaatgatag
     4381 gccgatctag acgtgatgga tttaatccga tgcgtattat tttagcgggt gcagcagtaa
     4441 gtgctatgtt aacagcgcta agtcaaggta ttgcattagc ttttagacta aatcaaacag
     4501 taacattttg gactgctgga ggcgtttcag gcacaacatg gtcacacctt aagtgggcaa
     4561 ttccattaat tggtattgcg ttattcatta tattaacaat tagtaaacaa cttaccattt
     4621 taaatcttgg tgaatcatta gctaaaggtt taggtcaaaa tgtaacaatg atcagaggca
     4681 tatgtttaat tattgctatg attctagcag gtattgcagt tgctatcgct ggacaagttg
     4741 catttgtagg tttgatggta cctcatatag caagattttt aattggaact gattatgcta
     4801 aaattctacc attaacagcc ttgttaggtg ggatactcgt gcttgttgcc gatgtgatag
     4861 cacgatattt aggagaagcg cctgttggtg caatcatttc atttatcggt gttccttact
     4921 ttttatattt agttaaaaaa ggaggacgct caatatgatt agttcaaata ataaacgcag
     4981 acaattgata gcactggctg tttttagcat tctactattt ctaggttgta cttggagtat
     5041 tacctcaggt gaatacaaca tacctgttga aagatttttc aaaactttaa ttggacaagg
     5101 tgatgccatt gatgagttaa tcttattaga tttcaggtta cctcggatga tgattactat
     5161 tttggctggc gcagcgctta gtattagtgg tgcaatagtg caaagtgtca caaaaaatcc
     5221 aatagctgaa ccaggtatat taggtattaa cgcaggtggc ggatttgcaa tcgcattatt
     5281 tattgcaatt ggtaaaatta atgctgacaa ctttgtttat gtactgccgt taataagtat
     5341 actaggtggt atcaccactg cattgattat ttttattttc agttttaata aaaatgaagg
     5401 tgttacacct gcgagtatgg tattaatagg tgtaggttta caaacagcat tatatggtgg
     5461 ctcaattaca attatgtcaa aatttgatga taagcaatct gatttcatcg ctgcttggtt
     5521 tgcaggtaat atttggggtg acgaatggcc atttgtcatt gcatttttac cgtgggtgtt
     5581 gattattatt ccttacttac tatttaaatc gaatacacta aatattattc atacgggtga
     5641 taatattgca cgaggtctag gtgtaaggtt aagcagagaa cgtttaatat tattctttat
     5701 cgcagtgatg ttatcatctg ctgctgtagc agtagcaggt tcaatttcgt ttatcggatt
     5761 aatgggtccg catattgcca aacgtatcgt tggaccacgt caccagttgt ttttaccaat
     5821 tgccatttta gtaggggcat gtttacttgt tatagctgat acaattggca aaattgtatt
     5881 acaaccaggt ggggttccag caggtattgt cgtagcaatt attggtgcac cgtatttctt
     5941 atatttaatg tacaaaacga aaaatgtata gtgtcaaagg acacaactta ttgctatgaa
     6001 aggcacttta ttataaggct tttcatagca ttttttattt aatgagccac tcaagactat
     6061 ttattttttc aataatgaac cattaagtta tcaagaggat cttatcaaaa atatatttga
     6121 taacggtatc aggttaattc tttatgatag cgcattcatt tattctgttt tatactatga
     6181 ctgataatac caaggaggta caacatgatg aaaaagttaa tcaataaaaa agaaacattt
     6241 ttaactgata tgcttgaagg attgttaatt gcgcacccag agttagatct gattgctaat
     6301 acagttattg taaaaaaagc taagaaagaa catggtgtag caatagtctc tggaggtgga
     6361 agcggacatg aacctgcgca tgccggtttt gttgcagaag gtatgctaga tgcagcggtt
     6421 tgtggcgaag tatttacatc acctacacct gataaaatat tagaagctat taaagcagta
     6481 gatactggtg atggtgtatt actagttgta aaaaactatg caggtgacgt gatgaatttc
     6541 gaaatggcac aagagcttgc agaaatggaa ggtataaatg ttcaaactgt tattgttcgt
     6601 gacgacattg ctgtgacaaa cgaagtacaa cgtcgtggtg ttgcaggaac agtgtttgtt
     6661 cataagcttg ccggttatct tgctgaaaaa ggttattcat taacagagat aaaatcgcgt
     6721 gtagaagcgt tgttacctga aattaaaagt attggtatgg caattgagcc accgcttgtt
     6781 ccaactactg gaaaatatgg ctttgatatt gaagacgaca aaatggaaat cggtattggt
     6841 atacatggtg aaaaaggtat tcatagggaa gaagtaaagg atattgatca tattgttgga
     6901 acattgttag acgaattgta taaagaagtt actgccaatg atgtcatatt aatggtaaat
     6961 ggtatgggtg gtacgccgtt atctgaatta aatatcgtaa ctaaatatat tcaacaaaat
     7021 ttagctgcaa gaacggttaa tgttgctaaa tggtttgttg gtgattatat gacatcttta
     7081 gacatgcaag gtttttctat aactatcgtg cctaataaac cagaatattt ggaagcattt
     7141 ttagcaccaa caacaagtca atactttaaa taagaagtga atatgaataa atatacattt
     7201 atgaggtggc acaaatgaaa gtgaatgata tgaaagcacg tttattaaat ttagaagaaa
     7261 cgtttaaaaa acatgaatct gaattaactg aattagatcg agcaattggt gatggtgacc
     7321 acggggttaa catggttcgt gggtttagta gtcttaaaga caaacttgat gatagctcaa
     7381 tgcaatcatt gttcaaatcg actggtatgg cattgatgtc aaatgttggg ggtgcatcag
     7441 gaccactgta tggctttagc tttgttaaaa tgtctgcagt caccaaagat gatatggata
     7501 atcaagattt cattacacta attcaggcat ttgccgaagc ggttgaatca cgtggtaaag
     7561 ttactttaaa tgaaaagaca atgtatgatg tagtagcgcg agcagcagag aagcttgaaa
     7621 atggtgaaac tttaacattc aatgatttac agcaattagc agataataca aaagatatgg
     7681 tagcaacgaa aggtagagct gcatattttg gagaagaatc aaaaggttat attgatccag
     7741 gtgctcaaag tatggtttat attttaaacg ctttgattgg agatgaagat aatgcctaaa
     7801 attatacttg ttagccacag taaagaaatt gcaagtggta caaaatcttt gttaaagcaa
     7861 atggcaggtg acgttgatat tataccaatc gggggattac cagatggttc aattggaact
     7921 tcatttgata tcatccaaga agttttgact aaattagagg atgatgcatt gtgtttttac
     7981 gatattggat cttcagaaat gaatgtagat atggcaattg aaatgtatga tggtaatcat
     8041 cgtgtgttaa aagttgatgc accaattgtt gaaggcagtt ttatcgcagc agtaaagcta
     8101 tcaatcggcg gttcaattga tgatgcatta gcagaaatca aacaatcatt ttagttaaaa
     8161 tttactaata atgaaaaatg taaacctttt tcaaatgaaa ctttataaaa aatatgatag
     8221 tatatatgta aatgtttaat aaaatctgga gaaataggag gacattgcca tgcaacacct
     8281 tataaaaaaa catgtattga atggcgagtt tgatttagta cgacaattga tgtccgaaac
     8341 agattttatg gaatttgaag aagcatatat ttcaagtgcg catgaagtag aaagtatgat
     8401 gttttataca tgtattttag atatgattaa gtacgaagaa tcatctgaaa tgcatgactt
     8461 agcattttta ttgcttgtgt atccactaag tgaatatgaa ggtgctttgg attctgctta
     8521 ttatcatgca gacgcttcca taaaacttac tgacggcaaa gaagttaaaa gtttgttaca
     8581 aatgttatta ttgcatgcga taccaacacc tgttatttca gataagaagg cttttgatat
     8641 cgccaagcaa attttaaaat tagatcctaa taataatgtt gctcgtaacg tcttaaaaga
     8701 cactgccaaa cgtatggaca acgttgttgt tgatataaat gaattacacc aacgtaatgc
     8761 acgttaatta catttcaatt atattagctt aataatagtt ttaacatttg gttgggttgg
     8821 gacatatgtt ccagcctttt ttaatactta aaaactaacg aagtatactt gtgtgcacaa
     8881 atggttttta tacaacattt tataaattta tacattttaa taaagaacat acgatatatg
     8941 gtttaaacct tgttaactga gagattttga tatgtattct tcgaaattta actaaattta
     9001 cgaaattcaa gaagcacaat aattaatcaa ttttcctata caaaagttcg tatgactgca
     9061 ttataaaagc ataaatttat aattttttta aatgtcattg aacgtgataa tgtgaatgga
     9121 ttgagcaatt ttgaaaaagt gaaaaataac ctatgcgact tgcaattaat tttcagtacg
     9181 ttataatgca cactgtgcaa aattaaggag gtctattatt cacatgatga tgaataaaga
     9241 agcaacaaaa attggatttg cctacgtcgg cattgtagtg ggcgcaggat tttcaactgg
     9301 acaagaagtt atgcaatttt tcactaaata tggcttgtgg gcttatttag gtgttattat
     9361 atctggtttt attttagctt ttattgggcg ccaagtagca aaaattggta ctgcctttga
     9421 agcgacaaac catgaatcaa cattacaata cgtattcggt gaaaagttta gtaaagtctt
     9481 tgattatatt ttaatcttct tcttatttgg tatagctgta accatgatag ctggtgcagg
     9541 cgcaacattt gaagaaagtt ataacatacc tacatggcta ggtgctttaa ttatgacatt
     9601 agcgatttat attacgttgc tattagactt taataaaata gtacgtgcac ttggtatcgt
     9661 tacaccattt ttaattgttt tagttgtatt aatcgctggc gtttatttat ttaaaggtca
     9721 tgtttcatta gcagaagtta accaagtagt gcctgaagca agtatttgga agggaatctg
     9781 gtttggtaca atatatggtg gattagcttt ttctgtaggt tttagtacca tcgtagcaat
     9841 cggtggggat acagaaaagc gtacagtgtc aggtgcaggc gcgatgtatg gtggtattat
     9901 ctatactgta ttactagcat tgatcaactt tgcattgcaa agtgaatatc caactattaa
     9961 aaatgcctca attccaacat tgacgttagc aaataatatc catcctttaa tagcaacagt
    10021 gttatctgtt attatgctgg cggttatgta taatactatt ctaggactaa tgtattcatt
    10081 tgcagcacgt tttacagaac catacagtaa aaattatcat atctttatta ttataatgat
    10141 ggtagcaggt tatttattaa gtttcgtagg atttgctgaa ttaattaata agttatatac
    10201 aattatggga tatgtaggct tatttattgt agtagctgta attattaaat atttcaaacg
    10261 taaaaatgcg gataaaaaac atattgctta atatcatatg atggatatcc gaaactttac
    10321 aattgaatca ctttgtttta accttaaaag c
//
LOCUS       DAOBBH010000087        10141 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.087, whole genome shotgun sequence.
ACCESSION   DAOBBH010000087 DAOBBH010000000
VERSION     DAOBBH010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10141
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.087"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(16..1026)
                     /locus_tag="P6L01_001565"
     CDS             complement(16..1026)
                     /locus_tag="P6L01_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HDP3030635.1"
                     /translation="MSIKILQPGLFSTVQDLGRIGYEHIGFSGAGAMDQFSFKVAQSL
                     INNDGPAIEYTLIGPTIQFNTQNTFVITGGSINASLNNKTISMNSVILAEKGDILKIG
                     AITKGARGYLTFGHSINVPSIAESYATHTRSSIGGFKGRKLLANDVITVKINNDFKEN
                     IGKTIHLQDDLLPENNIIHILQGPQFEAFSEEARAKIVNHPYLITEQSDRMGYRLEGD
                     SVAPINQADIISEPVALGSVQVPNDGQPIILLNDKQTIGGYTKIATVCKFDLPKLAQM
                     KPKDTIQFKWISFQEAVDKNREQMSLFNEILKSHQKTPIFDTSSLRHTSKKLSTILKG
                     DL"
     gene            complement(1016..1750)
                     /gene="pxpB"
                     /locus_tag="P6L01_001566"
     CDS             complement(1016..1750)
                     /gene="pxpB"
                     /locus_tag="P6L01_001566"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDP3030636.1"
                     /translation="MDVRFINEQTIMIYFENKISEETYRNVTAMVRWIREKEILEIQD
                     IVPSYRAVLIYFDEQAITSSKLIENLELNKFNEKNVHAVNQTNRIIKIPVQYGGTYGP
                     DIEEVAKHNRITVEQVIEKHTSKPYLIYMLGFMPGFPYLGGLDEQLHTPRRNQPRLKI
                     HAGSVGIANNQTGLYPSDSPGGWQIIGRTPLKVFSSEREPMSMYEAGEWIQFYAIDEQ
                     KFIQIERDISDGNFNVDDWVVIENVN"
     gene            complement(2076..2552)
                     /gene="greA"
                     /locus_tag="P6L01_001567"
     CDS             complement(2076..2552)
                     /gene="greA"
                     /locus_tag="P6L01_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDP3030637.1"
                     /translation="MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGD
                     LSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIEDTGDNNVVKIGKTVTFVELPGDE
                     EESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ"
     gene            complement(2580..3203)
                     /gene="udk"
                     /locus_tag="P6L01_001568"
     CDS             complement(2580..3203)
                     /gene="udk"
                     /locus_tag="P6L01_001568"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDP3030638.1"
                     /translation="MKATTIIGIAGGSGSGKTTVTNEIMKNLEGHSVALLAQDYYYKD
                     QKHLTFDERLETNYDHPFAFDNDLLIENLKDLKNGKAVEVPTYDYASHTRSDITIDFK
                     PKDVIIVEGIFALENKVLRDMMDVKIYVDTDADLRILRRLTRDTKERGRSMDSVINQY
                     LSVVRPMHDQFIEPTKKYADIIIPEGGSNKVAIDIMTTKIQSLVSKQ"
     gene            complement(3203..4471)
                     /locus_tag="P6L01_001569"
     CDS             complement(3203..4471)
                     /locus_tag="P6L01_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDP3030639.1"
                     /translation="MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLG
                     GQEYGLRSNADNFTMEEIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKT
                     GATGIIVADPLIIETCKEVAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGA
                     MEMREMKEKVDIEIEAFIHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLE
                     VDDNGELDVFYNQGEVTPFAMSPKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATV
                     VSVYRKVIDAYAADPDNFKINPEWLIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSK
                     KSPFDFCGLVLDYNEDTKIATIQQRNNFKPGQEIEFFGPEIETFTQVVEAIYDEEGNS
                     LDAARHPLQIVQIKVDRPIYPNNMMRKEIG"
     gene            complement(4483..5406)
                     /locus_tag="P6L01_001570"
     CDS             complement(4483..5406)
                     /locus_tag="P6L01_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDP3030640.1"
                     /translation="MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQ
                     MTEAVALAHKNDKKVYAAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVK
                     AQENPIPLHWNAETLVTNHFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQ
                     VQGMTCMFQSKRMLLGNYYTFQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTH
                     IMSPNDICLIEELAPFFEAGIDSFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYE
                     DEKFMLIDPIEEIQPDHRPFDEGFLYKQTVY"
     gene            complement(5409..6047)
                     /locus_tag="P6L01_001571"
     CDS             complement(5409..6047)
                     /locus_tag="P6L01_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDP3030641.1"
                     /translation="MDDLNKKYLIDLHQHQNSSIEVLREFAEGNEVPIVDRLTLDLIK
                     QLIRMNNVKNILEIGTAIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFEN
                     QVRIIEGNALEQFENVNDKVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYH
                     GFVSDIGIVRSRNVRQMVKKVQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE"
     gene            complement(6332..6640)
                     /locus_tag="P6L01_001572"
     CDS             complement(6332..6640)
                     /locus_tag="P6L01_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDP3030642.1"
                     /translation="MTEHNHDSQLEINNEEELLTLFDEEGNEVLYRKVLEFYHPEFKK
                     EYVILAEEGAQSDEDDMIELVPMINEPDESGDGGKLVPIETDEEWDMIEEVVNTEMEE
                     "
     gene            complement(6655..7083)
                     /gene="ruvX"
                     /locus_tag="P6L01_001573"
     CDS             complement(6655..7083)
                     /gene="ruvX"
                     /locus_tag="P6L01_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDP3030643.1"
                     /translation="MLQHKILGLDVGSRTVGIAISDIMGWTAQGLDTLRINEENNELG
                     IEQLVDIIKKHNVGTVVIGLPKNMNNSIGFRGEASLTYKEKLLEAYPSIEIVMWDERL
                     STMAAERSLLEADVSRQKRKQVIDKMAAVFILQGYLDSLH"
     gene            complement(7087..7347)
                     /locus_tag="P6L01_001574"
     CDS             complement(7087..7347)
                     /locus_tag="P6L01_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDP3030644.1"
                     /translation="MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVG
                     YLLSGDPAYIPRQNEARNQIRHIDRDVIMEELVSYYLKEQNK"
     gene            complement(7410..10040)
                     /gene="alaS"
                     /locus_tag="P6L01_001575"
     CDS             complement(7410..10040)
                     /gene="alaS"
                     /locus_tag="P6L01_001575"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDP3030645.1"
                     /translation="MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSG
                     VATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQ
                     EAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
                     IGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYT
                     PLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFK
                     VIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVAD
                     IMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAF
                     KLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLK
                     NITSASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAVSGGQVAD
                     TGIVYNDNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHS
                     ATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKG
                     IDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFK
                     IVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQMKVKSDDQVVEKLTQLQDE
                     EKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSK
                     LQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGG
                     TQPENISKSLSFIKDYIKNL"
BASE COUNT         3015 a         1978 c         1387 g         3761 t
ORIGIN      
        1 ttttcaatat tcattttaca aatccccttt taaaattgtt gataattttt tcgaagtatg
       61 tcgcaagcta gatgtatcaa aaattggagt cttttgatga ctcttaagaa tttcattaaa
      121 cagagacatt tgttcccgat tcttatctac agcttcttgg aatgatatcc atttaaattg
      181 aattgtatct tttggtttca tctgtgctaa ctttggcaga tcaaatttgc atacagttgc
      241 aattttggta taaccaccta tcgtttgttt atcattaagc agaataatag gttgaccatc
      301 atttggtact tgaacactac caagagcaac cggttcagaa atgatatctg cttgattaat
      361 tggtgcaacg ctgtcacctt ccaaacgata gcccatacgg tctgattgtt cagtaattaa
      421 atatggatga tttacaattt tcgctctagc ctcttcagaa aatgcctcga attgaggtcc
      481 ttgaagaatg tgtataatat tattttctgg caataaatcg tcctgtaaat gaatagtctt
      541 tccaatgttt tctttaaagt cattatttat tttcactgtt attacatcat tagctaataa
      601 ctttctacct ttgaatcctc ctatactgct tcgggtatgt gttgcataac tttcagcaat
      661 agaaggtacg ttgatagaat gaccaaaagt aagataaccg cgtgcacctt tggttatagc
      721 acctattttt aaaatgtcac ctttctcagc taatatgaca gaattcattg atatagtttt
      781 attatttagc gaagcattaa tactaccacc ggttataaca aatgtatttt gcgtattaaa
      841 ttgaatggta ggaccaatca aagtatattc aatcgctgga ccatcattgt taattaatga
      901 ctgcgcaacc ttaaaactaa attgatccat ggcacctgcg cctgaaaatc caatatgttc
      961 ataacctatt cttcctagat cttgtaccgt tgaaaagaga cctggttgta aaatcttaat
     1021 tgacattttc aatcaccacc cagtcatcaa cattaaagtt gccatctgat atatctcttt
     1081 cgatttgtat aaatttctgt tcatctattg cataaaattg tatccattct cctgcttcgt
     1141 acattgacat tggttcacgc tcgctgctaa atacttttaa cggtgtgcgt ccaataattt
     1201 gccatccgcc aggagaatct gatggatata gtcctgtttg attattcgca atacctacag
     1261 aacctgcatg aatttttaac cttggctgat tacgtctagg tgtatgtagt tgttcatcaa
     1321 gtccgcctaa gtatggaaat cctggcataa atcctagcat atatattaaa taaggtttac
     1381 ttgtatgttt ttcaataact tgctcaacag ttattcgatt atgctttgct acttcttcaa
     1441 tatctggtcc atatgtacca ccatattgaa caggtatttt aataatacga ttggtttgat
     1501 tcacagcatg aacatttttt tcattaaatt tgttaagttc taaattttca attaatttag
     1561 aagatgttat agcttgttca tcaaaatata ttagaactgc tcgatacgaa gggacaatat
     1621 cttgaatttc taatatttct ttttctcgta tccaccgtac cattgctgtg acattacgat
     1681 atgtctcttc ggatatttta ttttcaaaat aaatcataat tgtctgctcg ttaataaatc
     1741 ttacatccac tttaaatccc cctttgtatt gcaataaacc agtattgaat accttttcat
     1801 tgtatcattg agaagcacaa gttgtttaat aagtaattca aatcgcatac aataacaaat
     1861 gataatcaat ttgttttctc ggaaatattt tgctttttta aaatggagta gttaatattg
     1921 taactcttac actaatttag gttctgctat cattcggtct gatggaaaat ttttactttt
     1981 catctgtccg attttttgat tttgaatata aaaaagcacg accgaagtat cattaacaca
     2041 cttcaatcgc gcaattaaac aatctatttg atcatttatt ggatattaac aatttttacg
     2101 ttcatttcgc caccattagg tagtggaaca cgaacttcat catctaaacc tttaccaatt
     2161 aacgctttag ccattggtga ttcatttgaa atcttaccat taaatgcatc tgattcagct
     2221 gaaccaacga tttgataact ttcctcttca tcacctggta attctacaaa cgttactgtt
     2281 ttaccaattt taacaacgtt gttatctcca gtatcttcaa tgattaatgc atttcttaac
     2341 atatgctcaa ttctttgaat atcttgttcg atgaatcctt gttcatcttt tgctgcatca
     2401 tactcagagt tctctgataa gtcaccaaat gaacgtgcaa ctttaatttt ctctacaact
     2461 tcaggacgct taactgtttt taattcttca agttcacgct ctaatttttc aaaaccttct
     2521 tgagtcattg gatattgctt ttgattttcc atattgtcat cttcctttac tgaattatac
     2581 tattgcttgc taactaaaga ctgaattttt gttgtcataa tatctattgc aactttattg
     2641 ctcccacctt caggaataat tatatcagca tatttcttag tcggttcaat aaattggtca
     2701 tgcataggtc taacaacact taaatattga ttgataacag agtccattga acgcccacgc
     2761 tctttagtat ctcgtgttaa acggcgtaat attctcaagt ctgcatctgt atcaacatat
     2821 attttaacat ccatcatatc acgtaatacc ttattttcta aagcgaaaat accttctacg
     2881 ataataacat ctttaggttt aaaatcaatg gtaatgtcac ttcttgtatg actagcataa
     2941 tcatatgtcg gtacttctac tgctttacca tttttcaagt ctttaagatt ttcaattaat
     3001 aaatcattat cgaatgcaaa tggatggtca taattggttt ctaggcgctc gtcgaaagtc
     3061 aagtgctttt gatctttata atagtaatct tgagcaagta aagcgacact atgaccttct
     3121 aagtttttca taatttcgtt agttacagtt gtttttcctg agccagatcc accagctatg
     3181 ccaatgattg tagtagcttt cattagccaa tttcctttct catcatgttg tttggatata
     3241 tcgggcgatc cactttaatt tgaacgattt gtaatggatg gcgcgccgcg tctaagctgt
     3301 taccttcttc atcataaatt gcttctacta cttgtgtaaa tgtttcaatt tctggaccaa
     3361 agaattctat ttcttgacct ggtttaaagt tatttcgttg ttgaatagtc gcaatttttg
     3421 tatcttcatt atagtctaat accaaaccac aaaaatcaaa tggtgatttt ttagattgtt
     3481 gttgaccaaa catctgttct tcataaccag gtgttccctc aaagaatgct ggtgcagtgt
     3541 ctctatttgc acatttatct aactcaatta accattccgg attaatctta aagttgtcag
     3601 gatctgccgc atacgcatca atgactttac gatatactga gacaactgtt gcaatataat
     3661 gaattgactt catacgtcct tcaattttta atgagtccac accaatatcc atcatttgag
     3721 gaattgattc gattaatttt aaatctttag gactcatcgc aaacggtgta acttcacctt
     3781 gattataaaa tacatcaagt tcaccattat catcaacttc taataattca taatcccaac
     3841 ggcaactttg acagcaaccg cctctgttgg aatcccttgc agtcatatga ttacttaatg
     3901 tacatctacc tgaataggcg atacacatag caccatgaat aaatgcttcg atttcaatat
     3961 ctactttttc cttcatttca cgcatttcca tcgcgccggt ctcacgtgct aatacaacac
     4021 gatccaatcc ttcttctttc caatattcta cagctttgta attagaaagt gattgttgag
     4081 tagataaatg aatttcaagt tttggcgcaa cttctttaca tgtttcgata attaaaggat
     4141 ctgcaacaat gatacctgtc gcaccagtct tttccaaatt acgcaaatat gattctagac
     4201 cttcaatatt ctcatcatgt gcaataatat ttgtcgtaac ataaattttg gcaccgtaac
     4261 ggttcgcaaa ttcaacacct tcagctattt cttccatcgt gaaattatca gcatttgaac
     4321 gtaatccata ttcttgacca cctaaaaata cggcatcagc gccataatgt actgctattt
     4381 ttaacttttc taagtttcca gcaggtgcta ataattctgg tttcttcata actgttttag
     4441 gagttgattt aatctcttct attgtcttca tgattaacct ccttaatata ctgtttgttt
     4501 atataagaaa ccttcgtcaa atggtcgatg atcaggttga atttcttcaa ttggatcaat
     4561 caacataaat ttctcatctt catagatttc aggatcttca ttgtacaaat ctatcgcttg
     4621 acgatactgt tccgttacca cattaatata ttcttccgtt tgtagaatac catcgatttt
     4681 aaatgaatct atacccgcct caaaaaatgg tgctaattct tcaattaaac aaatgtcgtt
     4741 tggtgacata atgtgcgtac cattgtaatc ttcgtaaact gggtaattat tttgtctttc
     4801 ttcatcataa agtaataaag attgttcatc attgcgacgt tcaattttca tttggcgatc
     4861 ttggaacgta taataattgc ctagtagcat acgctttgat tggaacatac aagtcattcc
     4921 ttgaacttgc acctcaattt ccacatttga attttctttt atattaataa tttcatccaa
     4981 attcagctca cgtgctaaga cagctcttga tgcgcctctt ttaccccagt aattacattg
     5041 aaaatgatta gttactaacg tctctgcatt ccaatgaagt ggtattggat tttcttgcgc
     5101 cttcacatac attactactg ctggatcccc gaaaataatt ctgtcaactc gtatttcatg
     5161 taaaaaatta atataatctt ctacagcatc taaatgataa ttatgaaata atccattcac
     5221 tgccgcatat acttttttat cgtttttgtg agctaatgcg acagcctctg tcatttgttg
     5281 tctattgaat tcccctggaa gtcttaaacc aaacttttgc tcgccaatta caaaagcatc
     5341 tgcacctaaa tcaataagtg tttccatatg gcttaatgac ttgggtgtga caagtaattc
     5401 tgtcatagtc attctccttt aattgaaatc gctaatccat cgtctatatt taaaaaattc
     5461 gttgtatatc ctggttgctt tattaaccac tcattataat cttgaacctt tttaaccatt
     5521 tgtcttacat ttctcgatct aacaatccca atatccgata caaaaccgtg atataaaaca
     5581 ttatctgtaa ttacgagacc ttggtgcttt aaaagtggtg tatatatttc aaaaaatttc
     5641 tttgattgcg cttttgctgc atcaataaat atcatatcat aaactttgtc atttacattt
     5701 tcaaattgct ctaaagcatt accttcaata attcgaacct ggttttcaaa atgataagta
     5761 gctaaatttt gtttagcata ttgaatcatc gtttcattac gctctatcgt tgtgacatga
     5821 atgtcatcag atatagaagc gaattgcata gaactatagc cgattgctgt accaatttct
     5881 aaaatatttt taacattatt catacgaatt aattgcttaa ttaaatctaa tgttaaacga
     5941 tctacaattg gcacttcatt tccctcggca aattcacgca aaacttcgat tgaactattt
     6001 tgatgttgat gtaaatctat taaatatttt ttatttaggt catccatgtt ttaaacttcc
     6061 tttatgtaaa ataagtcaat atgattatga caataaaata aatcagcctt cacaattgat
     6121 tataattttg ccaaccaatt aaatgactga tttcttgtta gacgcaaagc tattttattt
     6181 atagaagcga atcattcata taaaatttaa ctttagatat tttaccatat tttcaataaa
     6241 attataagcg ttaattattt atacattgct tgacttaaaa aaatactctt gcctacccat
     6301 cttaaggtta gcaagagtaa aatcttttta attattcttc catttcagta tttacaactt
     6361 cttcaatcat gtcccattct tcatcagttt cgattggtac taacttacca ccgtcacctg
     6421 actcatctgg ttcattgatc attggtacaa gctcaatcat atcgtcttca tctgattgag
     6481 caccttcttc agctaagata acatactctt ttttgaattc aggatgataa aattctaaaa
     6541 cttttcggta taaaacttca tttccctctt catcgaataa agttaataat tcttcttcgt
     6601 tattaatttc tagttgtgaa tcatgattat gttcagtcat agtaaaatct ccttttaatg
     6661 tagtgaatct aaatagcctt gtaaaataaa taccgctgcc attttatcaa tcacttgttt
     6721 tcttttttgt cttgaaacat ctgcttctaa taatgatcgt tcagcagcca ttgtgcttaa
     6781 tctttcatcc cacatcacaa tctcaataga aggataagct tctaataatt tttctttata
     6841 tgttaacgaa gcttcgcctc gaaatcctat tgaattattc atgtttttag gtagtcctat
     6901 tacgactgta cccacattat gttttttaat aatgtctact aattgctcaa tacctaattc
     6961 attattttct tcattgattc ggagtgtgtc taatccttgt gccgtccaac ccattatatc
     7021 actaattgca attcctaccg ttctactacc gacatcgagt cctaaaattt tatgttgtaa
     7081 cataaattat ttattttgct cttttaaata gtaagaaaca agttcttcca taataacatc
     7141 tctatcaata tgacgaattt gatttcttgc ttcattctgg cgtggaatat acgcagggtc
     7201 acctgataat aaataaccta caatttggtt tacggcatta tatcctcgtt catctaatgt
     7261 tcgataaaca ttatttaaaa catctcttac atcttgcgtt ggaagttctt catagtcgaa
     7321 tttcattgtt ttatcaaagt tttccatttg cgacactcct ttaattacaa atataactca
     7381 ctatcatcat acaatattat ggctttaaat tatagatttt taatgtaatc tttaataaag
     7441 cttaatgatt ttgagatatt ttcaggttgt gtaccgccac cttgagccat atctggacga
     7501 ccgccacctt taccaccaac gattggtgcc atttgtttga taagatcacc ggctttaacg
     7561 ttatttgtta aagatttagg gacagttgca accatcgata ctttatcatc aacattactt
     7621 gcaagaatga taattgtatc ttgtagttta gatttaaaat catccattgt cgagcgaatt
     7681 gctttcgcat ttggtacatc cacttcagta accaatactt tatagccatt gatttcttca
     7741 acttgatctt caatattacc cattttaagt gatgtgattt ctttgtcacg ttgctctaat
     7801 tgttttaata atgctttttc ttcatcttgt aattgtgtta acttttcgac tacttgatca
     7861 tcagatttca ctttcatctg tgatttcatc gtattaaatt tctcttgaat atcttctaaa
     7921 tataagaaag ctgctttacc tgttaatgct tcaatacgac gcacaccagc tcctgtacct
     7981 gactcactta ctattttgaa taagccaatt tcagaagtat tgcggacatg aataccacca
     8041 cataattcaa ttgaaaatgg tgccatattt actacacgca caacatcacc atatttttca
     8101 ccgaataatg ccattgcgcc catttcttta gctgaagcaa tatccatttc ttgaatgtta
     8161 acgtcaatac ctttccaaat ttcttcattt actaagcgtt caacttgatc aatttcatca
     8221 ttagtcattg gaccaaaatg agagaaatca aaacgtaaac gatctgcttc tactagtgaa
     8281 ccagcttggt taacatgatc acccagtact gatttcaacg ctgcatgtaa taaatgtgtt
     8341 gcactatggt tcttttgaat gtcacgtcga tcattttggt tcacttcagc agacactgta
     8401 gcgccaacat ttacttggcc aaattgtact actcctttat gcaagttttg accatttggt
     8461 gctttggtta cttcactaac agcaatttca aaattgtcat tataaacaat acctgtatcc
     8521 gcaacttgtc caccactgac tgcataaaat ggtgtttccg ttaacatgaa gtatactgtt
     8581 tcacccgctt caacttgtga aacttcttca ccattatata tcaagtgtgt tagtgttgtt
     8641 tgagctgtcg cagtatcata accaacaaaa gtacttgcag atgtaatatt tttcaatact
     8701 tcactttgaa cttgcattga ttgagaattt tgacgtgctt gacgtgcacg atcacgttgt
     8761 tgttgcattt ctgactcaaa tgttgtcata tcaactttca atcctgcttg cactgctatt
     8821 tcttcagtta attcaattgg gaacccgtac gtatcataca atttaaatgc atctttccca
     8881 ttaatttcat ttgttgtcgc tttagctttt ttaattaatt catttaaaat cgctaaacca
     8941 tcttctaatg tttcatggaa tcgttcttct tcagacttta taacacgctt aatgaaatct
     9001 gctttttcct taacatttgg ataatatggt tccataatgt ctgcaacaat atcaacaagt
     9061 ttgtacataa atggctcatt gattcctaac gtttgactaa aacgaacggc acgacgtaac
     9121 aatcgacgta atacatatcc tctaccttca ttggcaggta atgcaccatc agaaattgca
     9181 aatgcaatcg tacgaatgtg gtcagcaatt actttaaatg ccacatcttg ttcgttgttt
     9241 actaaatatt gtttacctga tactttttcg atttcattca ttataggcat aaataaatct
     9301 gtttcatagt tagtacgtac attttgagaa actgaggcca tacgctcaag ccccatgccg
     9361 gtatcaatat ttttattagg taatggtgtg taactatgat ctttattatg attgaattca
     9421 ctaaatacta agttccatac ttcaagatag cgttcatttt ctccacctgg atacatttct
     9481 tctgccggat cgtcttgtcc atatgcttct ccgcgatcat agaaaatctc agtgttcggt
     9541 cctgaaggcc cttcaccaat atcccagaag ttaccttcaa tgcgaataat acgactttct
     9601 tcaagcccaa tatctttatg ccaaatgtta tatgcttcca tatcttccgg atgaatcgta
     9661 acgtacaatt tatctggctc catacccatc catttatcac tcgttaaaaa ttcccaagca
     9721 aattcaatcg cttcttgttt aaaataatca ccaattgaga agttacctaa catttcaaag
     9781 aatgtatggt gacgcgctgt gaaaccaaca ttttcaatat catttgtacg aatagctttt
     9841 tgagagttta caattcttgg ctttttaggt gtttcacgtc catcaaaata tttctttaat
     9901 gttgctacac ctgaattaat ccataataat gtatcatcat caattggcac taatggtgca
     9961 gaaggttcaa ccatatgtcc tttttcaaca aagaaatcta gatatttttg tctaatttca
    10021 ctcgctttta actttttcat cttttacaca tcctatttac tgtttttaaa ttaccattcc
    10081 ataaaaattg atgacacaga tagtcgattt gaaaaactag tataaatcaa tatcattttt
    10141 t
//
LOCUS       DAOBBH010000088        10028 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.088, whole genome shotgun sequence.
ACCESSION   DAOBBH010000088 DAOBBH010000000
VERSION     DAOBBH010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10028
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.088"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(56..772)
                     /locus_tag="P6L01_001576"
     CDS             complement(56..772)
                     /locus_tag="P6L01_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030646.1"
                     /translation="MRINDKILLENIEDYFNHKGLSPHLIDDIKEKVITDIKNSEKKD
                     QDYIEYKRKSPAQIILMIQRNLFALQMNPVIFFIINFILISYLYDKQYVQFQAITGMS
                     LFYCLVIFPMTIVVYLRVSQKNYLRSNKIEMIMGTIIAIISLLLIILQAFNITWGVIP
                     ITNFGHQFFFFIGIILVIAGIFYKRLEFSGIGLLFCQKTVDAMIHNPQSAQIFSLIIW
                     ILLVVLVIYFTIRLSSRTRL"
     gene            complement(934..1440)
                     /locus_tag="P6L01_001577"
     CDS             complement(934..1440)
                     /locus_tag="P6L01_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030647.1"
                     /translation="MKKLLTASIIACSVVMGVGLVNTSAEAASGNSIDTVKQLIKGDQ
                     SLENVKIGESIKDVLTKYKNPMYSYNEDGTEHYYEFHTKKGMLLVTTDGKKNNGKVTH
                     ISMMYNDANGPTYQAVKNYVGKAVTHTEYSKVAGNFGYIEKGKTTYQFASAPKDKNIK
                     LYRIDLEK"
     gene            complement(1576..2376)
                     /locus_tag="P6L01_001578"
     CDS             complement(1576..2376)
                     /locus_tag="P6L01_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDP3030648.1"
                     /translation="MDLFTRKDGTSIHYSTLGEGYPIVLIHTVLDNYSVFNKLAAQLA
                     KSFQVVLIDLRGHGYSDKPRHIEIKDFSDDIVELLKYLYIEEVAFVCHEMGGIIGADI
                     SVRYPEFTSSLMLVNPTSIEGELPEERLFRKYAHIIRNWDPEKQDKFLNKRKYYRPRK
                     MNRFLKHVVDTNEISTKEEIQAVKEVFKNADISQTYRNVVVPTKIIAGEFGERTTRLE
                     AKEVADLIQNADFEVYQESSAFPFVEEQERFVEDTASFINKHHDEKHV"
     gene            complement(2360..3079)
                     /locus_tag="P6L01_001579"
     CDS             complement(2360..3079)
                     /locus_tag="P6L01_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDP3030649.1"
                     /translation="MDLNQIKAVVFDLEGTLLDRVKSREKFIEEQYERFHDYLIHVQL
                     ADFKKAFIELDDDEDNDKPDLYKEIIKRFHVDRLTWKDLFNDFEMHFYRYVFPYYDTL
                     YTLEKLSQKGFQIGVIANGKSKIKQFRLHSLGLMHVINYLSTSETVGFRKPHPKIFED
                     MIDQLGVLPEQIMYVGDDALNDVAPARAMGMVSVWYKQEDAEIEPLEEEVDFTITTVE
                     ELLTILPIKNDNKGENYGSIY"
     gene            complement(3134..4084)
                     /locus_tag="P6L01_001580"
     CDS             complement(3134..4084)
                     /locus_tag="P6L01_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDP3030650.1"
                     /translation="MTFNKVLLSWIVILIITTSIYLFWQLGDINDVFNQSILINVRLP
                     RLLEALLTGMILTVAGLIFQTVLNNALADSFTLGLASGATFGSGLALFLGLTTLWIPV
                     FSITFSLITLITVLVITSVLSQGYPVRILILSGLMIGALFNSLLYFLILLKPRKLNTI
                     ANYLFGGFGDAEYSNVSIIAITFIIALFGIFIILNQLKLLQLGELKSQSLGLNVQLIT
                     YIALCIASMITAINVAYVGIIGFIGMVIPQLIRKWQWKQSLGRQLALNIVIGGQIMVM
                     ADFIGSHILSPVQIPASIIIALIGIPVLFYMLISQSKRLH"
     gene            complement(4233..5120)
                     /locus_tag="P6L01_001581"
     CDS             complement(4233..5120)
                     /locus_tag="P6L01_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDP3030651.1"
                     /translation="MKKSLIAFILIFMLVLSGCGMKDNDKQGSDDNGSSKSPYHRIVS
                     LMPSNTEILYELGLGKYIVGVSTVDDYPKDVKEGKKQFDALNLNKEELLKAKPDLILA
                     HESQKATANKVLSSLEKQGIKVVYVKDAQSIDETYNTFKQIGKLTHHDKQAEQLVEET
                     KDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGW
                     NPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSR
                     PGPRIDEGLKELRDAIYRK"
     gene            complement(5372..6007)
                     /locus_tag="P6L01_001582"
     CDS             complement(5372..6007)
                     /locus_tag="P6L01_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HDP3030652.1"
                     /translation="MLGTDELYKLLYRHMGPQNWWPADNDIELMLGAILVQNTRWRNA
                     EIALNQIKEHTHFNPNHILELPIETLQSLIHSSGFYKSKTLTIKTLLTWLARHHFNYQ
                     EINERYKGGLRKELLSLKGIGSETADVLLVYIFGRIEFIPDSYTRKIYDKLGYENTKN
                     YDQLKKVVTLPNHFTNQDANEFHALLDVFGKHYFRDKDIKNYDFLEPYFKK"
     gene            complement(6277..7938)
                     /locus_tag="P6L01_001583"
     CDS             complement(6277..7938)
                     /locus_tag="P6L01_001583"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDP3030653.1"
                     /translation="MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDY
                     ATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAII
                     PEAIEKGDQFGHVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYN
                     VTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHP
                     EIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKGEILEVLAK
                     MKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILI
                     DLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLR
                     EIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAK
                     EQGIEVTAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLAAHFH
                     KFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM"
     gene            complement(7935..8351)
                     /locus_tag="P6L01_001584"
     CDS             complement(7935..8351)
                     /locus_tag="P6L01_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDP3030654.1"
                     /translation="MDKKVSIQTKQVLKQHNEKEKFEFTTEGTWQQRQSNFIRYVEQI
                     EDATVNVTIKVDDDSVKLIRKGDINMNLHFVEGQTTTTFYDISAGRIPLEVKTLRILH
                     FVSGDGGKLKIHYELYQDNEKMGSYQYEINYKEIGE"
     gene            complement(8444..8552)
                     /locus_tag="P6L01_001585"
                     /pseudo
     CDS             complement(8444..8552)
                     /locus_tag="P6L01_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499172.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8627..9637)
                     /gene="adhP"
                     /locus_tag="P6L01_001586"
     CDS             complement(8627..9637)
                     /gene="adhP"
                     /locus_tag="P6L01_001586"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HDP3030655.1"
                     /translation="MRAAVVTKDHKVSIEDKKLRALKPGEALVQTEYCGVCHTDLHVK
                     NADFGDVTGVTLGHEGIGKVIEVAEDVESLKIGDRVSIAWMFESCGRCEYCTTGRETL
                     CRSVKNAGYTVDGAMAEQVIVTADYAVKVPEKLDPAAASSITCAGVTTYKAVKVSNVK
                     PGQWLGVFGIGGLGNLALQYAKNVMGAKIVAFDINDDKLAFAKELGADAIINSKDVDP
                     VAEVMKLTDNKGLDATVVTSVAKTPFNQAVDVVKAGARVVAVGLPVDKMNLDIPRLVL
                     DGIEVVGSLVGTRQDLREAFEFAAENKVTPKVQLRKLEEINDIFEEMENGTITGRMVI
                     KF"
BASE COUNT         3212 a         1876 c         1259 g         3681 t
ORIGIN      
        1 aaaaaactat tcatctcaat taatacaatt aatcaagtga atagttttat catatttata
       61 atcttgtacg tgaagataat ctaattgtga aatatataac tagaactact aataatatcc
      121 atataattaa tgaaaaaatc tgggctgatt gtggattatg aatcattgca tcgacggttt
      181 tttgacaaaa taataagccg attcccgaaa actcaagtcg cttataaaat atgccggcaa
      241 ttactaaaat aataccaatg aaaaagaaaa attgatgtcc aaaatttgta attggtataa
      301 cgccccaagt aatattaaat gcttgtaata taattaataa caaggatatg atagcgataa
      361 ttgtacccat aatcatttct attttattac tacgcaagta attcttttgt gacaccctta
      421 ggtatacaac aatagtcatt ggaaaaatca ctaaacaata aaatagactc attccagtaa
      481 tagcttgaaa ctgaacatac tgtttatcat ataaatagga tatgagaatg aagtttataa
      541 taaagaaaat aactggattc atttgtaaag caaataaatt cctttgaatc attaatatga
      601 tttgtgctgg agattttctt ttatattcaa tataatcttg atctttcttt tcagaatttt
      661 ttatatcagt aattactttc tctttaatat catcaatcaa atgcggtgat aaacctttat
      721 gattaaagta atcttctata ttttctagta aaattttatc gttaatacgc attggtagta
      781 ctcctttaaa atgaaacatc tttatttatt gtattggttt aatttcccct tcgcaaatca
      841 taaaaattaa tattcactgt attatgccat gtaataaaat aaccgacaag tattgcgatt
      901 taacttgtcg gttaagcacg attaatttaa ttattatttt tccaaatcaa tacgatataa
      961 ttttatgttt ttatcttttg gtgctgaggc aaattgataa gtcgttttgc ctttttcaat
     1021 atatccaaaa ttaccagcaa ctttgctata ttccgtatgt gttactgctt tgccaacata
     1081 atttttaaca gcttgatatg ttggaccatt agcatcatta tacatcattg aaatatgagt
     1141 tactttacca ttgtttttct taccatcagt agttactaat aacatacctt ttttagtatg
     1201 gaattcgtaa taatgttcag ttccatcttc attgtaagaa tacataggat ttttgtactt
     1261 agttaaaaca tctttaatag attcgccaat tttcacattt tctaatgact gatcaccctt
     1321 aattaattgt ttaacagtat caatagagtt gccacttgct gcttcggcac tagtgttcac
     1381 taagcctact cccattacaa cagaacatgc aattatactt gccgttagta gctttttcat
     1441 aagcactctc tccttttatt tatatcgtct tgtgctcaca accattatac aacaatcatt
     1501 tctaaaacaa cagtcatatt acaattatat tacaaataat aattactact tttatatttc
     1561 acttatcact aaaaattaaa catgcttttc atcgtgatgt ttgttgataa atgaagctgt
     1621 atcttcgacg aatctttctt gctcttcaac aaatgggaat gcacttgatt cttgatatac
     1681 ttcaaagtcc gcattttgga ttaaatcagc tacttcttta gcttccaatc ttgttgttct
     1741 ttcaccgaat tctcctgcaa taatttttgt cggtactacg acatttctat aagtttgaga
     1801 aatatcagcg tttttgaata cctctttaac tgcttgaatt tcttctttag ttgatatttc
     1861 atttgtatct acgacatgtt taaggaatcg attcattttt ctcggacgat aatacttacg
     1921 cttatttaaa aatttatctt gtttttcagg atcccagttt cgaataatat gggcatattt
     1981 tctaaataaa cgttcttccg gtaattcacc ttcaatagat gttggattta ccaacataag
     2041 tgatgatgta aattcaggat aacgtactga aatatccgca ccaatgattc cacccatttc
     2101 atggcataca aatgcaactt cttcaatgta taaatattta agtaattcaa caatgtcatc
     2161 agaaaaatct tttatttcaa tgtgacgagg tttatcagaa tagccatgtc cacgtaaatc
     2221 aattaacaca acttgaaatg attttgctaa ttgtgctgct aatttattaa acacagaata
     2281 attatcaagt acagtatgaa tcaatacgat aggatagcct tcacctaatg tactgtaatg
     2341 tatcgatgtt ccatcttttc tagtaaatag atccataatt ttctccttta ttatcatttt
     2401 ttattggtaa aatggttaat aattcttcca ctgttgtaat tgtaaaatca acttcttctt
     2461 cgagtggttc aatttcagca tcttcttgtt tataccatac actaaccatg cccatagctc
     2521 gtgctggagc tacatcattt aacgcatcat cgccaacata cataatttgc tcaggtaata
     2581 cccctagttg atcaatcata tcttcaaaaa ttttaggatg tggtttacga aaaccaactg
     2641 tttctgatgt tgataaataa ttaataacat gcatcaaacc aagtgaatgt aatcgaaatt
     2701 gtttaatctt agatttacca tttgcgataa caccaatttg aaagcctttt tgcgatagct
     2761 tttctagtgt atacaaagta tcgtaataag gaaatacata acgataaaaa tgcatttcaa
     2821 aatcattaaa taagtctttc caagttaacc tatctacatg gaaacgttta atgatttctt
     2881 tatataaatc aggtttatca ttatcttcat cgtcatctag ctcaataaat gcttttttaa
     2941 aatctgccag ttgaacatga attaagtagt catgaaatcg ttcatattgc tcttcgataa
     3001 atttctctcg agatttaact ctgtccaaca acgtaccttc taaatcaaat acaactgctt
     3061 taatttgatt caaatccatc gcttatgccc tctttatgtt tatagttatt tttattttag
     3121 caaatgtcat gtgctagtgt aaccgtttcg actgagatat tagcatgtaa aataacactg
     3181 gtataccaat taatgcaata ataatgcttg ccggtatttg tactggtgac agtatatggc
     3241 taccaataaa atctgccata accattattt gtcctccaat tacaatattc aaagccaatt
     3301 gtcttcctaa tgattgtttc cactgccatt ttctaatgag ttgtggtatc accataccaa
     3361 tgaatccaat gatgccaaca tatgcgacat ttatcgccgt tatcatagaa gctatacata
     3421 acgcgatata tgtaatcaat tgaacattta agcctagtga ctgacttttt agttctccta
     3481 attgcaataa ctttagttga ttaagaatga taaatatacc aaacagtgca atgataaatg
     3541 tgattgctat tatggataca tttgagtatt ctgcatcacc aaagccacca aacaaataat
     3601 tggcaattgt atttaatttg cgaggtttta ataaaatcaa aaaatataga agtgaattga
     3661 ataatgcacc aatcattaaa ccacttaata ttaagattct aactggatag ccttggctca
     3721 ataccgacgt aatgactaat acagttatta atgttatcaa actaaatgtt attgaaaata
     3781 caggaatcca taacgttgtt aaacctaaaa ataatgctaa tcctgaacca aatgtagcac
     3841 cgcttgccaa tcctaatgta aagctatctg ccaatgcatt atttaaaact gtttgaaata
     3901 taagacctgc aactgttaat atcatacctg tcaacaatgc ttctaataat ctcggtaatc
     3961 taacattgat taaaatagac tggttaaata catcattgat atcccccaac tgccaaaata
     4021 gatatatgct agttgttata atcaatatga ctatccagct caataatact ttattaaatg
     4081 tcatagcatt ctcttttctt tcatcgataa ttacattcat tatagcaatc acatactcgc
     4141 gttgctatca ttcactaaat atattttaaa aaccccttat ctcaaacatg tattttttta
     4201 catgtagcaa taaggggcat aattagaatg gtttattttc tataaattgc atctcttaat
     4261 tcttttaatc cttcatcaat acgtggacct ggtcttgata cttcatcacc atttacaact
     4321 tcaatacgtg tattcttgac agcattaatt ttattgaatc cacccctttt tttgatgata
     4381 tccatataat ctgatcttgt cttagcttcc gtcgaaatta atatatctgg gttcttttta
     4441 ataatacttt ccttcgttac agggttccaa ccattaatgt cactataaac attttgggct
     4501 tctaattttt ctaacatatc attaaaaaat gtatgcttcc ctgctgtata tatttcaggc
     4561 tttgatgaaa cctcaataaa tacttttgat tttttatgat gagcaggaat tgaatctatg
     4621 actttatcga tattatcttt agtttcctca acaagttgtt cagcctgctt atcatgatgc
     4681 gttaatttcc caatttgctt aaatgtgttg tatgtttcat caattgattg tgcatcttta
     4741 acatacacta ctttgatgcc ttgtttctct aatgatgaca atactttatt agcagttgcc
     4801 ttttgcgact catgcgcaag aattagatct ggctttgcct ttaaaagttc ctctttattt
     4861 agattcaaag catcaaattg tttcttaccc tctttcacat cttttggata atcatcaacc
     4921 gttgaaacac caactatgta tttacctaat cctaattcat ataaaatttc agtattacta
     4981 ggcattaacg aaacaattct atggtacggc gatttagacg agccattatc atcgctacct
     5041 tgtttatcat tatctttcat accacagcca ctcaggacaa gcataaaaat caaaataaaa
     5101 gcaattaacg atttcttcac attatcacat ctccttttta atcccacttt tcgctttagc
     5161 gattagaggg atttatgcag ttgtgcctta gcatccaact gcgttcctct tcacctttag
     5221 tgattagagg cttttatgca gttgctgcac aaacaaacaa ctgttttttt ctttatatgc
     5281 ttctatttat tatataagtc cacatactaa aagaaaatag taaattattt tttatatttc
     5341 atataaactc atctatctaa cttcacagcg tttacttttt aaagtaaggt tctaaaaaat
     5401 cataattctt tatatctttg tctctaaagt aatgtttacc aaatacatct aacagagcat
     5461 gaaattcatt agcatcttga tttgtaaaat gatttggtaa tgtgactact ttttttaatt
     5521 gatcataatt tttagtgttt tcatatccta atttatcata tatttttctt gtatagctat
     5581 ctggaataaa ttcaatacgt ccgaatatat aaacaagtaa gacatctgct gtttcacttc
     5641 caataccttt caaagataat aattcttttc ttaatccacc tttatatcgc tcattaatct
     5701 cttgataatt gaaatgatgt cgtgctaacc atgttaataa tgttttaatc gtcagtgttt
     5761 tacttttata aaagccactt gaatgtatca atgattgtaa cgtttcaata ggtagttcta
     5821 atatatgatt gggattaaaa tgcgtatgtt ctttaatctg attcaatgca atttctgcat
     5881 ttcgccatct agtattttga actaaaattg cacctaacat cagttcaatg tcattatcag
     5941 caggccacca attttgcggt cccatatgcc tatataacaa tttatacaat tcatcagttc
     6001 ctaacattta caccctcctt ttagagcccc taattaataa attaattagg ggctcttatg
     6061 cagttgctgc gcaagcaagc caactgtatt cctttactcc ccttgagctt tagcgattta
     6121 ggctctctta tgcagttgtg cagtagcatc caactgcgtc cctcttaacc ttattcacat
     6181 actaagcatt ccccaatatg tcacatacct aattaaaaac actccaacac aaagtatctt
     6241 tcgagaaact ctacgttgga gtgtatataa atgttcttac attgattcag gtgcgcttac
     6301 accgaccatt gccaatgcat ttttcaatgt aattctgacc gcttcaatca tagcaacatg
     6361 tgcttttgtt ttttcaatat catctgttaa cactttttca gcattataga atttatggaa
     6421 atgagcagcc aagtcttgaa tatagttagt aattctatgt gctgatctat gctcagcagc
     6481 actttcaatt gtaggttcga aatcagctac ttttttcaac aattcaatcg ctttttcatt
     6541 tgtaatcgtt gtaaaatcat tcgcagcagt cacttcaata ccttgctctt tcgcttgttt
     6601 taaaattgaa caaatacgcg catgtgcata ttgagcatag taaactggat tgtcttgaga
     6661 ttgctctttc gctaattcca tatcaaaatc aaagtgacta tcaggactac gcatagttaa
     6721 gaaataacgt gcagcgtcaa cgccaacttc gtccataatt tctcttaatg taatcgcatt
     6781 accagtacgt ttactcatct tcacttcttt accattttcc attaaacgaa ccatttgcat
     6841 gatttgaatt tctaaacgat tactatctac accaaacgtt tcaagagatg ctttcaaacg
     6901 attaatataa ccatgatgat cagcaccaaa taaatcgatt aaaatgtcat taccacgttt
     6961 aactttatcg aagtggtacg caatatctgg taagaaatac gtatatgtac cgtcattttt
     7021 aattaatact ctgtctttgt cgtctttaaa atcagttgta cgtaaccatg tagcgccatc
     7081 agcttcatac gtataaccta attctttcat ttttgctaaa acttcaagaa tttcgccttt
     7141 ttcatataaa gatgtttcac taaaccaatt atcaaaatgc gtattgaact ctgctaaatc
     7201 atttttcaat ttagccattt cgtattctac gcctaatttt ctaaattctt tcaaacgtgc
     7261 ttcttcagaa taatctttaa tttcagggtg tttctctgct aaatctttac ctatttcaat
     7321 aatatctttt ccattatagc catcttctgg cattgaataa ctattgtcac ctaaagcttc
     7381 aaagaaacgt gtttcaatcg aacgcgctaa gttagtaatt tgattaccag catcattaat
     7441 ataatattca cgtgttacat tatagccagc tgcagttaaa atattagcta aagcatcacc
     7501 aactgctgca tttctagcat gaccaatatg taaatctcct gtagggttag ctgaaacata
     7561 ctcaagcaat acattttgac cttttgattc atttacatgt ccaaattgat cacctttttc
     7621 aattgcttca ggaataattg ctgttaaata ctgattatct aagtaaaaat taatgaatcc
     7681 tggaccagca atgtcaattt gttttacatg tgctttttca gtatctaagt tatcaacaat
     7741 cgcttgagca atttcacgag gattacgctt tgcaatctta gtcagtacca tcgcaatatt
     7801 agtagcataa tctccatttt ttgtatcttt aggaacttca attttaatat caggaatctc
     7861 atctgctaat cctgctttgt taatacttgc tgcaatttct tctactaatg tttgtttcac
     7921 ttgatcaata atattcattc gcctatctcc ttatagttaa tttcatattg ataagaaccc
     7981 attttttcat tatcttgata taattcataa tgaatcttta gcttgccacc gtctccactt
     8041 acgaaatgta aaatgcgtaa tgttttaact tctagtggaa ttcgtccagc cgatatatcg
     8101 taaaaagttg ttgtcgtttg tccttcaaca aaatgcaaat tcatattaat gtcgccttta
     8161 cgaatcaact taacgctatc atcatccact tttattgtaa cattaactgt tgcatcctca
     8221 atttgttcta cataccgaat aaagttagat tgcctttgtt gccaagttcc ttcagtagta
     8281 aattcaaatt tttctttttc gttgtgctgt ttcaacactt gctttgtttg aatacttact
     8341 tttttatcca atttactact caccttaatc ttcgcataat agaaatatta tagcataaaa
     8401 taaggtcatc ttgttatgcc ttcttaaagc taaataggtt caattaatta acaattccat
     8461 acttaatcaa cgcttcatat atgtaagcca ttttataaaa tacaaacaac acctcaaaac
     8521 caaaaaaaca cgagattttc actatctttc atgaaaacct cgtgtttgat tataatgata
     8581 gtgttcagag cactaccttc tttatctata tagtcagttg atatttttaa aatttaataa
     8641 ccattctacc agttatagta ccattttcca tttcttcaaa aatatcattg atttcttcta
     8701 attttcttaa ttgaacttta ggtgttactt tattttcagc agcaaattca aacgcttcac
     8761 gtaagtcttg tcttgtacca acaagtgaac ctactacttc aataccgtca agcactaatc
     8821 ttgggatatc taagttcatt ttatcaacag gtaaaccaac ggcaacaact cttgcaccag
     8881 cttttacaac atcaaccgct tggttaaatg gcgtcttagc aactgaagtt accactgttg
     8941 catctaatcc tttgttatca gttaatttca taacttctgc aactggatca acatctttag
     9001 aattaataat agcatctgca cctaattctt tcgcgaatgc taatttatca tcattaatgt
     9061 cgaaggcaac aattttagcc cccataacgt ttttagcata ttgtaaagct aggttaccta
     9121 aaccacctat accaaaaaca cctaaccatt gaccaggttt tacattactt acttttacag
     9181 ctttataagt tgtcacacct gcgcatgtaa tagaagacgc tgctgctgga tctaattttt
     9241 caggtacttt cacagcatag tctgcagtaa caataacttg ttcagccatt gcaccatcta
     9301 ctgtataacc agcatttttc acactacggc aaagtgtttc acgacctgtt gtacaatatt
     9361 cacatcttcc acagctttcg aacatccaag cgatagacac acggtctcca atttttaatg
     9421 attctacatc ttctgcaact tcgatgactt taccaatacc ttcatgacct aaagtaacgc
     9481 ctgtaacatc accaaaatca gcattcttaa catgtaaatc ggtatgacaa acaccacaat
     9541 attccgtttg taccaatgct tcaccaggtt ttaaagctct taactttttg tcctcaatac
     9601 ttactttgtg atctttcgtt acaactgctg ctctcataat aatgtcctcc ttatagtcac
     9661 tccagtatgt atgatattaa ttggcgatta ctttcaaaaa tgaaaacgaa taattataga
     9721 tgtaattttt atttgtaata gacgccagat taaaacatac tactgtgact ttgtttttag
     9781 ttagatatca accgttaact tttaacgttt ccctagttaa tatgttaaaa gaaatgacat
     9841 aatcgatatg ctaacgtgta atttttactt cacatatatt tatttaaaga taatttataa
     9901 tactcttatc ttcactctta ttataatgtg aattaattca caaagtatat agctatgatc
     9961 aaaaaaatgt gtcagttatt caaatttttt attaactaca gattaattta atttcaaaaa
    10021 aattaaaa
//
LOCUS       DAOBBH010000089        10007 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.089, whole genome shotgun sequence.
ACCESSION   DAOBBH010000089 DAOBBH010000000
VERSION     DAOBBH010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10007
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.089"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(145..237)
                     /locus_tag="P6L01_001587"
     CDS             complement(145..237)
                     /locus_tag="P6L01_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030656.1"
                     /translation="MTKNSKYTNEVSKEQFQEIENVNNKVKEFY"
     gene            201..335
                     /locus_tag="P6L01_001588"
     CDS             201..335
                     /locus_tag="P6L01_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030657.1"
                     /translation="MKLHWYIYYFLSISVILFMIIYHYLATSMTVDYEITVSIAKLLL
                     "
     gene            complement(540..1019)
                     /gene="rlmH"
                     /locus_tag="P6L01_001589"
     CDS             complement(540..1019)
                     /gene="rlmH"
                     /locus_tag="P6L01_001589"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDP3030658.1"
                     /translation="MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAP
                     ENMSDKEIEQVKEKEGQRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSD
                     FVFVIGGSNGLHKDVLQRSNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK"
     gene            complement(1387..3705)
                     /gene="adsA"
                     /locus_tag="P6L01_001590"
     CDS             complement(1387..3705)
                     /gene="adsA"
                     /locus_tag="P6L01_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adenosine synthase AdsA"
                     /protein_id="HDP3030659.1"
                     /translation="MKALLLKTSVWLVLLFSVMGLWQVSNAAEQYTPIKAHVVTTIDK
                     ATTDKQQVTPTKEAAHQFGEEAATNVSASAQGTADEINNKVTSNAFSNKPSTAVSTKV
                     NETHDVDTQQASTQKPTQSATFTLSNAKTASLSPRMFAANVPQTTTHKILHTNDIHGR
                     LAEEKGRVIGMAKLKTIKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDA
                     MAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVT
                     TPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGD
                     YLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALAQTGTALANIGKVTFNYRNG
                     EVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDV
                     RTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFG
                     NTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDMNKPSG
                     KRINAIQILNKETGKFENIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVLA
                     SYLKTANIAKYDTTEPQRMLLGKPAVSEQPAKGQQGSKGSESGKDVQPIGDDKAMNPA
                     KQPATGKVVLLPTHRGTVSSGTEGSGRTLEGATVSSKSGNQLVRMSVPKGSAHEKQLP
                     KTGTNQSSSPAAMFVLVAGIGLIATVRRRKAS"
     gene            complement(3932..4732)
                     /locus_tag="P6L01_001591"
     CDS             complement(3932..4732)
                     /locus_tag="P6L01_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDP3030660.1"
                     /translation="MSRLIRMSVLASGSTGNATFVENEKGSLLVDVGLTGKKMEELFS
                     QIDRNIQDLNGILVTHEHIDHIKGLGVLARKYQLPIYANEKTWQAIEKKDSRIPMDQK
                     FIFNPYETKSIAGFDVESFNVSHDAIDPQFYIFHNNYKKFTILTDTGYVSDRMKGMIR
                     GSDAFIFESNHDVDMLRMCRYPWKTKQRILGDMGHVSNEDAGHAMTDVITGNTKRIYL
                     SHLSQDNNMKDLARMSVGQVLNEHDIDTEKEVLLCDTDKAIPTPIYTI"
     gene            complement(5121..5909)
                     /locus_tag="P6L01_001592"
     CDS             complement(5121..5909)
                     /locus_tag="P6L01_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDP3030661.1"
                     /translation="MNWKLTKTLFIFVFILVNIVLVSIYVNKVNRSHINEVESNNEVN
                     FQQEEIKVPASILNKSVKGIKLEQITGRSKDFSSKAKGDSDLTTSDGGKLLNANISQS
                     VKVSDNNLKDLKDYVNKRVFKGSEYQLSEINSGSVKYEQTYDNFPILNNSKAMLNFNI
                     EDNKAASYKQSMMDDIKPTDGADKKHQVIGVRKAIEALYYNRYLKKGDEVINARLGYY
                     SVVNETNVQLLQPNWEIKVKHDGKDKTNTYYVEATNNNPKIINH"
     gene            complement(5910..7244)
                     /locus_tag="P6L01_001593"
     CDS             complement(5910..7244)
                     /locus_tag="P6L01_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030662.1"
                     /translation="MNNKEHIKSVILALLVLMSVVLTYMVWNFSPDIANVDNTDSKKS
                     ETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHV
                     RRDHNLMIPDLSSDFTLFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDHDADDN
                     IVLYAISKDRHDYVKLTTTTKNDHFLDALAAVKKDMQPYTDIITNKDTIDRTTHVFAP
                     SKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKY
                     HYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGY
                     PTFNKEGSNQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKTLPKLESVRSSLANNSDI
                     NFEKVTNIAIGYEMQDNSDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE"
     gene            complement(7237..9063)
                     /gene="walK"
                     /locus_tag="P6L01_001594"
     CDS             complement(7237..9063)
                     /gene="walK"
                     /locus_tag="P6L01_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDP3030663.1"
                     /translation="MKWLKQLQSLHTKLVIVYVLLIIIGMQIIGLYFTNNLEKELLDN
                     FKKNITQYAKQLEISIEKVYDEKGSVNAQKDIQNLLSEYANRQEIGEIRFIDKDQIII
                     ATTKQSNRSLINQKANDSSVQKALSLGQSNDHLILKDYGGGKDRVWVYNIPVKVDKKV
                     IGNIYIESKINDVYNQLNNINQIFIVGTAISLLITVILGFFIARTITKPITDMRNQTV
                     EMSRGNYTQRVKIYGNDEIGELALAFNNLSKRVQEAQANTESEKRRLDSVITHMSDGI
                     IATDRRGRIRIVNDMALKMLGMAKEDIIGYYMLSVLSLEDEFKLEEIQENNDSFLLDL
                     NEEEGLIARVNFSTIVQETGFVTGYIAVLHDVTEQQQVERERREFVANVSHELRTPLT
                     SMNSYIEALEEGAWKDEELAPQFLSVTREETERMIRLVNDLLQLSKMDNESDQINKEI
                     IDFNMFINKIINRHEMSAKDTTFIRDIPKKTIFTEFDPDKMTQVFDNVITNAMKYSRG
                     DKRVEFHVKQNPLYNRMTIRIKDNGIGIPINKVDKIFDRFYRVDKARTRKMGGTGLGL
                     AISKEIVEAHNGRIWANSVEGQGTSIFITLPCEVIEDGDWDE"
     gene            complement(9076..9777)
                     /locus_tag="P6L01_001595"
     CDS             complement(9076..9777)
                     /locus_tag="P6L01_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDP3030664.1"
                     /translation="MARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYE
                     EEPDIVLLDIMLPGRDGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTK
                     PFSTRELIARVKANLRRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTH
                     REFELFHYLSKHMGQVMTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEY
                     IVTRRGVGYFLQQHE"
BASE COUNT         2789 a         1975 c         1508 g         3735 t
ORIGIN      
        1 tacacccctt aattatattt tacatctatt tccattatta cattttatga gtctcgcaaa
       61 ttgtcagttt ttaaattatg ataattattt tccaaatagt ttattaaaaa actacatctt
      121 tcttgattga tactctttca aatcttaata aaattctttg accttattat ttacattctc
      181 aatttcttgg aattgttctt ttgaaacttc attggtatat ttactatttt ttgtcaatat
      241 ctgtaatttt atttatgatt atttatcatt acttagctac gtcaatgact gttgattatg
      301 aaataactgt ttctattgca aagttacttt tataatttaa taaggacaaa aagaagcatt
      361 ctatattaat cattttagat ataaaccaat ttgatagggc ctaatttcaa ctgttagcta
      421 ctacttaagt tatatgcgca attatcgtga tatatcttat atattgaatg aacgtggatt
      481 taatgtccac catttaacac cctccaaatt attatctcct catacagaat tttttagttt
      541 tacttatgat acgcctctcc acgcataatc ttaaatgctc tatacacttg ctcaattaac
      601 acaacccgca tcatttgatg tgggaatgtc attttgctga atgatagtgc gtagttactg
      661 cgttgtaaga cgtccttgtg caggccgttt gatccgccaa tgacgaatac aaagtcgctt
      721 tgcccttggg tcatgcgttg gttcaattct tgggccaatc cttcggaaga tagcatcttt
      781 ccttgtattt ctaatgtaat gactgtggat tgtggtttaa ttttggctag tattcgttgg
      841 ccttcttttt cttttacttg ctcaatttct ttgtcgctca tattttctgg tgctttttcg
      901 tctggaactt ctatgatgtc tatcttggtg tatgggccta aacgtttttc atattctgct
      961 atggcttgct tccaatattt ctcttttagt ttccctacag ctaaaatggt gattttcatg
     1021 tcgtttggtc ctccaaattg ttatcaactt tccagttatc cacaagttat taacttgttc
     1081 acactgttcc ctcttattat accaatattt tttgcagttt ttgatatttt cctgacattt
     1141 atgcccaatt tttagtgtat aacttgtgta tttgtggata actctgtggg cattgtgggt
     1201 atttctcttt aaattgtaca caattctgga taactatccc catttctgtg gataaacacc
     1261 ttgatgtctt attacttatc cccgttttta tatcccatgt ttatcaacgt ttatagcttt
     1321 tttccataaa aaaacactac cgtacctctt atttaagaaa tacagtagta ttgttttcaa
     1381 tatattttaa ctagcttttc tacgtcgtac agtcgcgatt aaacctatac ctgctactaa
     1441 tacaaacatc gctgctgggc ttgagctttg attagttcca gtttttggta actgtttctc
     1501 atgcgcgcta cctttaggca ctgacattct aaccaattgg ttcccactct tgcttgatac
     1561 agtagctcct tctaatgtgc gaccagaacc ttctgtaccg ctactaacag ttcctctatg
     1621 cgttggtaac aatacaactt tacctgtcgc tggttgtttc gctggattca tcgctttgtc
     1681 gtcaccaatt ggttgtacat ctttaccaga ctcactacct ttgctacctt gttgtccttt
     1741 agctggttgt tcacttactg ctggtttacc taataacata cgttgtggtt ctgtcgtatc
     1801 atacttagct atgttagctg tttttaaata acttgctagt acttgatcta atgaaatacc
     1861 ttcttctcta gggccaccga acatactata tccgtcgcca cctgatgctg tgaagtcatt
     1921 catcgttaca tgatatacac gttttaaatc aatattttca aacttacctg tctctttatt
     1981 taaaatttga atagcgttaa ttcgtttgcc agacggttta ttcatatcat agtaaacacg
     2041 aattgaatca gagatatgta gtaaaccgcc attcgctgtt aatactgtct taccgtcttt
     2101 ttgtgttgtt ggtgcaccta aactatgttc gaaagctgtc cagacgtctg aaccttttac
     2161 atcaatttgc gcaatcgtat ttccaaatgg taatactgag attaaatcat agcgtgtcac
     2221 cttacctttt gcgatagagg cacgaatacc tccaccattt gtcacggcaa agtcagtctt
     2281 tttagagaaa ttcttaacgc catacgcttc catagcatct gcaatcgcgt ttcctaaatt
     2341 tgtttcacgc gttctaacgt catctctttc tcctttgaaa tcaatggtat tatttggaat
     2401 aataacctct gctgtttgtg ctctaaatgt ttgatcagct tgattaattt gttcagctaa
     2461 tgctttgttc ggtgttacat tttcaacgtc tttaacatta atcaatgacg gtttaatatt
     2521 tgatacctct ccattgcggt aattaaatgt aaccttaccg atattcgcaa gtgctgtacc
     2581 tgtttgtgct aatgcatcat tgttataaat ttgaccattt tgaagtacgg tatgtgaatg
     2641 accatcaatg actgtaatac gtttcttcaa ttgtggattt tgacttaatt gtttcactaa
     2701 gtaatcacca cgccatgttt cttgtgttga aggatcaatc cctaaatgtg atataacaac
     2761 aaatgtatct acgtctttat aaatacgcat catttctgct gtcacacttt gtaatggatc
     2821 tctaaattca acacctttaa tgccctcagg tcttgttttc gtctttgttt ctggtgtcgt
     2881 tacgccaata attccataac gaataccatt tttcgttaca attgttgaag gcttaaacgc
     2941 gcgtttccca tctttgtaaa cgttagtact tagcatcggg aagtctaaca taccctctaa
     3001 ctttttcaac tgatcgtatc caaagtcaaa ttcatggtta cccactgcca tagcatcata
     3061 acctactgca ttcattgctt tagccatttc ttcaccttta gactggtttg aaagtggtaa
     3121 accttggaag gcgtctcctg cgtctaacat taaatcaggc ttttcttgtt cttttattgt
     3181 ttttaattta gccataccga tgacacgccc tttttcttcg gctagtcggc catggatatc
     3241 atttgtatgt aatattttat gtgttgttgt ttgtggtaca ttggcagcaa acattcgtgg
     3301 tgaaagtgat gctgttttag catttgataa tgtgaatgtt gctgattgag ttggtttttg
     3361 tgttgaggct tgttgtgtat ctacatcgtg cgtttcgttt acttttgttg aaactgctgt
     3421 agatggtttg ttagaaaatg cgttggatgt tactttattg tttatttcat cagctgttcc
     3481 ctgtgctgat gctgatacgt tggttgccgc ttcttcacca aattgatgag ccgcttcctt
     3541 tgttggcgtt acttgttgct tatctgttgt tgctttgtct atcgttgtta ctacatgtgc
     3601 tttgattggt gtatactgct cagccgcgtt cgagacttgc cataatccca tcacactaaa
     3661 aagcaaaacg agccatacac ttgttttaag taataaagct ttcatttcgt tccctccatt
     3721 aactgtcata caccaatata ttatcataat ccttatatta aaatgattga ttttatgtaa
     3781 attatttata aattacattt aatatcgaat tattttttac atttcaaata gaaccttaat
     3841 tccaacaaca gccatttccc accaataaca taaaaaagca cccgataaag tcgtctcaca
     3901 gcagtgccga actttatagg gtgactctca tttatattgt atatattggc gttggaatag
     3961 ctttatccgt atcacatagc aatacttctt tttccgtatc aatatcgtgt tcgttcaata
     4021 cttggccaac actcatacgc gccaaatctt tcatgttatt gtcttgtgat aaatgcgata
     4081 ggtaaatacg tttcgtgtta cctgtaatca catctgtcat cgcatgaccc gcatcctcat
     4141 tagatacatg acccatatcg cctaaaatac gttgtttcgt cttccatgga taacgacaca
     4201 ttctcaacat atcgacgtca tgattactct caaaaataaa cgcatcgctg ccacgtatca
     4261 tacctttcat acgatcagac acgtaacccg tatccgttaa aatcgtaaac ttcttatagt
     4321 tattatggaa aatataaaat tgcggatcta ttgcatcatg tgacacgtta aacgattcaa
     4381 catcgaaacc tgcaatagat tttgtttcat aaggattaaa aatgaatttc tgatccatag
     4441 ggatgcgact atctttcttt tcaattgcct gccaagtctt ttcattcgca taaattggca
     4501 attgatattt acgcgccaaa acacctaatc ctttaatatg atcaatatgt tcatgggtta
     4561 ctaaaatacc atttaaatct tgaatattac ggtcaatttg actaaacaat tcttccattt
     4621 tcttgccagt caaaccaaca tcaactaata gactaccttt ttcattttct acaaaagtgg
     4681 cgttacctgt actaccactt gctaatacac tcatgcgtat caagcggctc atcctttctc
     4741 tttaaacagt caatctctct atatgactgc aatatacatc agtgcaaata tacgattttc
     4801 cctacagaaa ctatcagtat attagctgtt ttgtatacct ttcctattat gtaactttag
     4861 ttcatcgacc ggtttcttat atttaaattt tgataatcac gtgcaatgaa tttcttaatt
     4921 attataacaa tgattagaca aatactaaag tttttaaaat agcagttacg ttaattttaa
     4981 aatgcggttc tacttaaaat atgaataaat gcgcacatta gagacgcaga aatgtcatcc
     5041 atgaaaaccc ataccagcaa aaagctagca ccgcttctca catatgatga gcttgcaata
     5101 ctagcttatt acgattcata ttaatgatta ataattttag ggttattatt tgtcgcttcg
     5161 acatagtaag tattcgtttt gtccttaccg tcatgcttca ctttaatttc ccagtttggt
     5221 tgtaacaatt gaacattcgt ttcgttcacg actgagtagt aaccgagtct agcattaatg
     5281 acttcatcac cttttttcaa gtaacgatta taatataatg cctcgattgc ttttctcact
     5341 ccaataactt gatgcttctt atctgcacca tctgtgggct taatgtcatc catcattgat
     5401 tgtttataac tagccgcttt gttatcttct atattaaagt tcaacatcgc tttactattg
     5461 ttcaaaatcg ggaaattatc atacgtttgt tcgtatttta cagaaccaga attaatttca
     5521 cttaattgat attctgaacc tttgaacaca cgtttgttaa cataatcttt caaatctttt
     5581 aagttattgt cactgacctt taccgattga ctaatgttcg cattcaataa ttttccacca
     5641 tctgatgtgg tcaaatccga atcgccctta gctttagaac taaagtcttt tgatcgccct
     5701 gtaatttgct ctaattttat acctttaact gatttattca atatactagc cggtacttta
     5761 atttcttctt gctgaaaatt aacttcattg ttactctcaa cttcattaat gtgtgagcga
     5821 ttgactttat taacataaat cgatactaac acgatgttga caagaataaa cacgaaaatg
     5881 aaaagtgtct ttgtcagttt ccagttcatt tattcaagcc tcccatcgtt ataaacatac
     5941 cattcaccat catattctac ataccaacgc ggtacgagtt cactgttaat ctgcacttca
     6001 atgtgattat gatctgagtt atcctgcatt tcgtaaccga tagcgatgtt tgttactttt
     6061 tcaaaattaa tatcactatt gttcgctaag cttgaacgta cagactctaa tttcggcaac
     6121 gttttattat cctcactatt taacacaacg tcggtgcgca ataacgaacg acgataatca
     6181 aagacgcctt tttcacccca agtgacttga atttgattag agccttcttt attaaacgtt
     6241 ggataaccgt taaggaaacg ttgatatgtt aactcgcctg actgattatt cgtactaaac
     6301 aatctgaagt cttcgtttaa gaaaccacca tgaccattaa taaaatcaaa ggttcctgga
     6361 atcgtttctt ccattttgct ggaacttgct tcatcttcgg acaggttttt ataatgatat
     6421 ttttcatttt tatcgttata gtttgcgaca cctgtattat tgttgtaggt tgtaacacca
     6481 ctctttgaac tacgaacgat ggttgaatcg tcaaatagta tagcattcat tttctcaaca
     6541 ctaatcgtgt taaataccat gcgatatgtt tttaactttt caggtttact tggtgcaaaa
     6601 acatgcgtcg tacgatcaat tgtatctttg tttgtgatga tatctgtgta tggttgcata
     6661 tcttttttca ctgctgctaa tgcatctaaa aaatgatcat ttttcgttgt agttgttaat
     6721 tttacgtaat cgtggcggtc tttacttata gcataaagca cgatattatc atcagcatca
     6781 tgatctatga ctaaacgatt gaaattgaaa tgatttggta ctttcgcatt catgttcagt
     6841 acttggccaa gatatgttga taacggtaaa tcatacgtaa aatcgaataa tgtaaaatca
     6901 ctgcttaaat ccggaatcat caagttatga tcacgacgaa catgttccac ggacttcact
     6961 tctttatttt tcaaaggttt cgtcagttta ttcacattag ataccgtcgc aatcgtacct
     7021 tctggatgat catttttcga atgaataatc tgaaatggcg taatagttgt atccattttg
     7081 gctgtcatag gtgtcgttaa aggtttcgtt tcactcttct tactatctgt attgtcgaca
     7141 tttgcaatat caggagaaaa gttccatacc atatatgtca atacgacact catcaagacg
     7201 agtagcgcta aaatgacaga tttaatatgt tccttattat tcatcccaat caccgtcttc
     7261 aatgacttca catggaagtg tgataaagat agatgtacct tgaccttcta cactgtttgc
     7321 ccaaatacga ccattgtgcg cttccacaat ctctttcgaa atggctagtc ctaatccagt
     7381 accacccatt ttacgcgtac gtgccttatc tacacgatag aatcggtcga atatcttatc
     7441 gactttattg ataggaatac cgatgccatt atctttaata cgaatcgtca ttcgattata
     7501 aagtggattt tgtttcacgt ggaactcgac acgtttatcg cctctagaat atttcatcgc
     7561 atttgtaatg acattatcga atacttgcgt cattttatca ggatcaaatt ctgtgaaaat
     7621 cgtctttttc ggaatatctc gaataaatgt tgtgtctttc gcagacattt catgtcgatt
     7681 aataatttta ttaatgaaca tgttaaagtc gataatttct ttgttgattt gatcagactc
     7741 attatccatt ttagataact gtagcaagtc attgaccagt cgaatcattc gttctgtttc
     7801 ttcacgggta acagataaaa attgtggcgc aagttcctca tctttccatg caccttcttc
     7861 aagtgcttca atgtaactat tcatagaagt taaaggtgta cgtaactcat gtgatacatt
     7921 ggcaacaaat tcacgacgct cacgttcaac ttgttgttgt tcagtaacgt catgtaacac
     7981 agcgatataa ccagttacaa atcctgtttc ctgcacaatc gtactaaagt taacacgtgc
     8041 gattagacct tcttcttcat ttaaatctaa taaaaaacta tcattattct cttgaatttc
     8101 ttcaagttta aattcatctt caagacttaa tacacttaac atgtaatatc cgatgatgtc
     8161 ttctttcgcc ataccaagca tcttgagtgc catatcattg acgatacgaa tacgtccacg
     8221 gcggtctgtt gcaataatac catcactcat atgggtgata actgagtcca gtctacgttt
     8281 ctcactttca gtattagcct gcgcttcttg tacacgttta gacaagttat taaatgctaa
     8341 agctaattcg ccaatttcat cattaccata aatcttcaca cgttgcgtat agttacctct
     8401 ggacatttcg accgtctggt tacgcatatc ggtgattggt ttggtaatcg ttcgcgctat
     8461 aaagaatcct aggatgactg tgattaataa tgaaatagct gtaccaacaa tgaatatttg
     8521 atttatatta tttaattggt tataaacgtc attaattttt gattcgatat aaatattacc
     8581 aattaccttt ttatcgactt taactgggat attatatacc cagacacggt ccttaccacc
     8641 gccataatct tttaaaatta aatgatcgtt tgattgtcct agtgatagtg ctttttggac
     8701 agaactatca ttcgcttttt gattgattag actacggtta gactgcttcg tcgtcgcaat
     8761 aataatttgg tctttatcta taaaacgaat ttctccaatt tcttgacggt tggcatactc
     8821 acttaataaa ttttgaatat ctttttgtgc atttacggag cccttttcgt catatacttt
     8881 ttcaatacta atttctaatt gtttagcgta ctgcgtaata ttcttcttaa aattatcaag
     8941 cagctctttt tcaagattat ttgtaaaata cagcccgata atttgcatac caatgataat
     9001 cagtaataca taaacaatta caagtttagt atgaagggat tgtagttgtt ttagccactt
     9061 cattcgtttc gacctctact catgttgttg gaggaaatat ccaacgcctc tacgcgtcac
     9121 aatatattca ggatgtgacg gatcatcttc aatcttttca cgtaaacgac gaatcgttac
     9181 atcgaccgta cgtacatcgc caaagtaatc atagccccat actgtttgta ataaatgttc
     9241 acgtgtcatt acttgtccca tatgttttga taaataatgg aacaattcaa attcacgatg
     9301 tgttaattca atatcttcgc cacgtttttt aatagaatat gcgtctggat aaatcacaat
     9361 atctttaatt gtgatttcat tcgttacatt tccagtgtct tgtgctggtt gtgagtaatg
     9421 acgacgtaag ttcgctttca cacgtgcgat taattcacgc gtactaaacg gtttcgttac
     9481 atagtcatct gcacctagtt ctaaaccaag cactttatca atttctgaat ctttagcagt
     9541 aagcattatt attggcattt cgtatttttt gcgcacttca cgacatactt ccataccatc
     9601 acgaccaggt aacatgatat ctagtaatac gatgtctggt tcttcttcat aaattaagtc
     9661 gactgcatca ttaccatcgt atgcacagta cacatcgtat ccttcttttt ttaagttaaa
     9721 ttctaaaata tcagcaatcg gtttttcatc atcaactaca acaacttttc tagccatttg
     9781 cataaacctc ttttcttaaa tcattaatat tattttatca ttttttgttc tttatcgttt
     9841 atgtttgtgt aaaaaatcac aggatgtcgc ttactatttt tgttattgtg cgcattttcc
     9901 gtcttataac gtatagtaac ctattatata atttaccatt tataagctat taattctatt
     9961 ttccaatata aacttatata ttaatatttt ttattagttt ctcacgg
//
LOCUS       DAOBBH010000090        10003 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.090, whole genome shotgun sequence.
ACCESSION   DAOBBH010000090 DAOBBH010000000
VERSION     DAOBBH010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10003
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.090"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..326)
                     /locus_tag="P6L01_001596"
     CDS             complement(<1..326)
                     /locus_tag="P6L01_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDP3030665.1"
                     /translation="MNIGNKIKNLRRIKNLTQEELAERTDLSKGYISQIESEHASPSM
                     ETFLNIIEVLGTTPSEFFKDSENEKVLYKKEEQVIYDEYDEGYILNWLVSKSNEYDME
                     PLILTL"
     gene            complement(470..745)
                     /locus_tag="P6L01_001597"
     CDS             complement(470..745)
                     /locus_tag="P6L01_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDP3030666.1"
                     /translation="MEYEYPIDLDWSNEEMISVINFFNHVEKYYESGVTAGDFMGAYK
                     RFKEIVPAKAEEKQIFNTFEKSSGYNSYKAVQDVKTHSEEQRVTAKK"
     gene            complement(913..2319)
                     /gene="lpdA"
                     /locus_tag="P6L01_001598"
     CDS             complement(913..2319)
                     /gene="lpdA"
                     /locus_tag="P6L01_001598"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDP3030667.1"
                     /translation="MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLG
                     GVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGG
                     VEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNAIIATGSRPIEIPNFKFG
                     KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQ
                     MTQPVKKGMKEKGVEIVTEAMAKSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRP
                     NTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAE
                     AIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLD
                     DTNGFVKLITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLG
                     EMTMEAAEKAIGYPIHTM"
     gene            complement(2323..3615)
                     /locus_tag="P6L01_001599"
     CDS             complement(2323..3615)
                     /locus_tag="P6L01_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDP3030668.1"
                     /translation="MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKS
                     VVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKE
                     EAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
                     YLNGGAPTASNESAASATSEEVAETPAAPAAVSLEGDFPETTEKIPAMRRAIAKAMVN
                     SKHTAPHVTLMDEIDVQALWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALN
                     TSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQISDEINELAVKARDG
                     KLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPV
                     LALSLSFDHRQIDGATGQNAMNHIKRLLNNPELLLMEG"
     gene            complement(3706..4683)
                     /locus_tag="P6L01_001600"
     CDS             complement(3706..4683)
                     /locus_tag="P6L01_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDP3030669.1"
                     /translation="MAQMTMVQAINDALKTELKNDQDVLIFGEDVGVNGGVFRVTEGL
                     QKEFGEDRVFDTPLAESGIGGLAMGLAVEGFRPVMEVQFLGFVFEVFDAIAGQIARTR
                     FRSGGTKTAPVTIRSPFGGGVHTPELHADNLEGILAQSPGLKVVIPSGPYDAKGLLIS
                     SIRSNDPVVYLEHMKLYRSFREEVPEEEYTIDIGKANVKKEGNDISIITYGAMVQESM
                     KAAEELEKDGYSVEVIDLRTVQPIDVDTIVASVEKTGRAVVVQEAQRQAGVGAAVVAE
                     LSERAILSLEAPIGRVAAADTIYPFTQAENVWLPNKNDIIEKAKETLEF"
     gene            complement(4687..5799)
                     /gene="pdhA"
                     /locus_tag="P6L01_001601"
     CDS             complement(4687..5799)
                     /gene="pdhA"
                     /locus_tag="P6L01_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDP3030670.1"
                     /translation="MAPKLQAQFDAVKVLNDTQSKFEMVQILDENGNVVNEDLVPDLT
                     DEQLVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILP
                     GYRDVPQIIWHGLPLTEAFLFSRGHFKGNQFPEGVNALSPQIIIGAQYIQAAGVAFAL
                     KKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAA
                     ETLAQKAIAVGIPGIQVDGMDALAVYQATKEARDRAVAGEGPTLIETMTYRYGPHTMA
                     GDDPTRYRTSDEDAEWEKKDPLVRFRKFLENKGLWNEDKENEVIERAKADIKAAIKEA
                     DNTEKQTVTSLMEIMYEDMPQNLAEQYEIYKEKESK"
     gene            complement(5970..6596)
                     /locus_tag="P6L01_001602"
     CDS             complement(5970..6596)
                     /locus_tag="P6L01_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkyA family protein"
                     /protein_id="HDP3030671.1"
                     /translation="MKFGKTIAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPI
                     KTVGKKIAELDEKKKKLTEDVNSKDTAVRGKAVKDLIKNADDRLKEFEKEEDAIKKSE
                     QDFKKAKSHVDNIDNDVKRKEVKQLDDVLKEKYKLHGEYAKAYKKAVNSEKTLFKYLN
                     QNDATQQGVNEKSKAIEQNYKKLKEVSDKYTKVLNKVGKEKQDVDQFK"
     gene            6961..7512
                     /locus_tag="P6L01_001603"
     CDS             6961..7512
                     /locus_tag="P6L01_001603"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDP3030672.1"
                     /translation="MLTMKDIIRDGHPTLRQKAAELELPLTKEEKETLIAMREFLVNS
                     QDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVKE
                     AYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLN
                     GVMFYDHIDKNHPLQPHTDAVEV"
     gene            7843..7965
                     /locus_tag="P6L01_001604"
     CDS             7843..7965
                     /locus_tag="P6L01_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030673.1"
                     /translation="MNDMMNLVINQIVIKLNYRDDWNEFIDIYSNTILLGLTIN"
     gene            7994..8212
                     /locus_tag="P6L01_001605"
     CDS             7994..8212
                     /locus_tag="P6L01_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDP3030674.1"
                     /translation="MAVFKVFYQHNRDEVIVRENTQSLYVEAQTEEQVRRYLKDRNFN
                     IEFITKLEGAHLDYEKENSEHFNVEIAK"
     gene            8212..9909
                     /locus_tag="P6L01_001606"
     CDS             8212..9909
                     /locus_tag="P6L01_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDP3030675.1"
                     /translation="MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPD
                     DNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALG
                     LIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVV
                     HTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKI
                     FRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPP
                     ETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPI
                     PGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGE
                     YRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDI
                     GNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKI
                     KTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESNNK"
BASE COUNT         3074 a         1865 c         1526 g         3538 t
ORIGIN      
        1 tttaaagtta atattaatgg ctccatatca tattcatttg actttgaaac taaccaattt
       61 aatatataac cttcatcata ctcatcataa ataacttgtt cttccttctt gtataatact
      121 ttttcatttt cactgtcttt aaaaaattca cttggcgtcg ttcctaacac ctctataata
      181 tttaagaaag tttccatact tggtgaggca tgttcacttt ctatttgtga aatgtagcct
      241 ttcgataagt ctgtacgttc agcaagttct tcttgcgtta aatttttaat tcttctaaga
      301 tttttaattt tattacctat gttcatttat ttttccacct aaaaaagcta tattaagttt
      361 acttttatca aacattttgt ttaacacttg ataaaggtat caaaatatag ctttattagc
      421 aatagtttta aagaaaaaat cctattaaat tgtgttaatt tcgaacgaat tattttttag
      481 ctgttactct ttgttcttca gagtgagttt ttacatcttg aactgctttg taactattat
      541 agccactact tttttcgaaa gtattaaaaa tttgtttttc ctctgcttta gcaggcacaa
      601 tttctttaaa tcttttatat gcacccataa agtcgcctgc cgtcacgccg gattcataat
      661 acttctctac atgattaaag aaatttatca ctgaaatcat ctcttcatta ctccagtcta
      721 aatcaattgg atactcatat tccattgttg aaccctccat ttcaattctt ttgttagcat
      781 cgctagtata acatgcatgt cacatgttaa cgatatctca aatcaatttc cttacattac
      841 tttacctaaa acaaaaaagc cgtagcatat gctacagctt ttaatgactg aatctttata
      901 gataatcagt tattacattg tatggattgg gtatccgata gctttttctg ctgcttccat
      961 agtcatctca cctaatgttg gatgtgcatg gattgttaat gcgatatctt cagcattcat
     1021 accagcttca attgctaaac ctaattcaga gataatatct gatgcaccag taccaactac
     1081 ttgagcaccg attaaagtat catcttcttt aagtgtaata agtttaacaa atccgttagt
     1141 atcatctaat gataatgcac gaccatttgc tgcatatggg aatttagaag ctttaattgc
     1201 taaaccttct tctttagctt gcgcttctga ataaccaact gtagctaatt ctggttcagt
     1261 aaagcatact gctggcatac caatgtaatc aacttcagca gcttgaccat caattgcttc
     1321 agcagcaact ttagcttcat agctagcttt gtgagcaagt ggtaaacctg gaacgatatc
     1381 accaattgca tagatattgc tgatagacgt acggctttgt ttatcaactt ctaataatcc
     1441 acggtcagcg aatttaacac ctaattcttc taggcctaat tcgtctgtgt ttggacgacg
     1501 acctacagtt actaatacat aatcagcttc gattgttttc tcttcgcctt tagcttcata
     1561 agtaacttta actccgttat ctgtttcttc agctgattta gccatagctt cagtaacgat
     1621 ttcaacacct ttttctttca tacctttttt aacaggttgt gtcatttgtt tttcgaagcc
     1681 acctaagata tctttagcac cttcaaggat ggttacttct gaaccaaagt tagcaaatgc
     1741 tgtacctaat tctgatccaa tgtatcctcc accaactaca actaatttac ctggtacttc
     1801 ttgtaagttt aaagcacctg ttgagtcgat aacacgttta ccgaatttga aattaggaat
     1861 ttcaattggt cttgaacctg ttgcaatgat tgcattttta aagttgtatg tttgtgcgct
     1921 cttttcgtcc ataacacgta agctattgtt atctacgaaa tatgcttcac ctttaacgat
     1981 gttaactttg ttacctttaa gtaagccttc aacaccacca gttaatttat taacaactga
     2041 tgatttgaat tcttgaactt tttggaagtt taaagaaaca ctttcagcaa taacacctaa
     2101 gttttcagaa tgttgtgctt caacaaaacg gtgagaagca tgtagtaatg cttttgaagg
     2161 aatacatcct acgtttaagc aaacaccacc aagattacct ttctcaacga ttgttacttt
     2221 ttgtcctaat tgagctgcac gaattgctgc aacgtatcca ccaggacctg ctccgattac
     2281 tatagtatct gtttcaattg ggaaatctcc aactaccatg ttttacccct ccattaataa
     2341 taattctgga ttatttaata aacgtttaat gtgattcatt gcattttggc cagttgcacc
     2401 atcaatttgt ctgtggtcaa agcttaatga taatgctaat actggtgctg caacaatttc
     2461 tccatcttta acgataggtt tttgagcaat acggccaatt cctaagattg ctacttctgg
     2521 gtgattgata actggagtga accattgtcc accagctgaa ccgatattac tgattgtgca
     2581 tgttgcacct ttcatttcat cggctgttaa tttaccatca cgtgctttaa cagctaattc
     2641 attaatttca tctgaaattt ggaaaataga cttacgatca gcatgtttca ctacaggtac
     2701 taataaacct ctatcagtgt ccgccgcaat accgatattc caataatgtt tatgcacaat
     2761 ttcaccagct tcttcattga atgaagtgtt aagtgctggg tattttttca atgcagaaac
     2821 aagtgcttta acaacataag gtaagaatgt taacttagta ccttgttcag ctgcgatttc
     2881 tttaaatttc ttacggtgat cccataatgc ttgaacatca atttcatcca ttaatgttac
     2941 atgaggtgca gtatgcttag agttaaccat tgctttcgca attgctctac gcatagcagg
     3001 gattttttca gttgtttctg ggaagtcgcc ttctaatgat actgctgcag gtgctgcagg
     3061 agtttcagca acttcttcac ttgtagctga agcagctgat tcatttgaag ctgttggtgc
     3121 accaccattt aagtatgcat ctacatcttc ttttgtaata cgaccatttt taccagatcc
     3181 agaaactgct ttaatgttaa cacctttttc acgtgcgtat ttacgtactg aaggcattgc
     3241 tttaacagtt ctgttttcat ctacttcttc agtttgagta gctacaggtg cttgctctgc
     3301 tggcgcttcc tctttcgcag gttcttcttt agatgatgaa tcatcatcat gacctttaaa
     3361 ttgcatatct tctgcatcag gtgcatcgat tttaacaata acgtcaccaa ctacagctac
     3421 tgtaccttct tctaccataa cttcttctac agtaccagat actggtgatg ggatttctac
     3481 tactgattta tcgttttgta cctcagctaa aacatcgtct tcttcaatag tatctccagc
     3541 tttaacaaac cattttacaa tttcaccttc gtggatacct tccccgatat cgggtaatct
     3601 aaattcaaat gccacgtttt tgccctccta agatttcgta aattaaacat ttcattttaa
     3661 tcaatgagac taaaatacgc taacttcgtt aacttttaaa atgtattaaa attctaaagt
     3721 ttcttttgct ttttcgatga tgtcattttt gtttggtaac caaacatttt cagcttgagt
     3781 gaatggataa attgtatctg ctgctgcaac tcttccaata ggtgcttcta atgaaaggat
     3841 tgcacgttca cttaattcag ctacaactgc tgcaccaaca ccagcttgac gttgtgcttc
     3901 ttgaactaca actgcacgac cagttttttc aactgaagct acaattgtgt caacatcgat
     3961 tggttgaaca gtacgtaagt caattacttc aacagaataa ccatcttttt caagttcttc
     4021 tgcagctttc attgattctt gaaccattgc accgtatgtg atgattgaaa tgtcattacc
     4081 ttcttttttc acattagcct taccaatgtc aattgtatat tcttcttcag gtacttcttc
     4141 acggaatgaa cgatacaatt tcatatgctc taagtatacg actgggtcat tacttctaat
     4201 agaagaaatt aataaacctt tcgcatcgta tgggcctgaa ggaataacaa cctttagacc
     4261 tggagattga gctaaaatac cttctaagtt atctgcgtgt aattctggtg tgtgtacgcc
     4321 accaccaaat gggctacgaa ttgttacagg tgcagtttta gtaccgcctg aacggaaacg
     4381 agtacgtgca atttgtccag caatcgcatc aaatacttcg aatacgaaac ctaagaattg
     4441 tacttccata accggacgga atccttcaac tgcaagaccc atcgctaaac caccaatacc
     4501 tgattcagct aaaggtgtat cgaatactct atcttcacca aattcttttt gtagtccttc
     4561 agtaacacgg aaaacaccgc cgttaacacc aacgtcttca ccaaaaatta aaacatcttg
     4621 gtcattttta agttcagttt taagcgcatc attaatcgct tgaaccattg tcatttgtgc
     4681 catggcttac ttcgactcct tctctttgta aatttcatat tgttctgcta agttttgagg
     4741 catatcttca tacataattt ccattagaga agtaacagtt tgtttttcag tgttatcagc
     4801 ctctttaatt gctgctttaa tatcagcttt tgcacgttca ataacttcat tttctttgtc
     4861 ttcattccat aaacctttgt tttcaaggaa tttacggaaa cgtactaatg ggtctttttt
     4921 ctcccattca gcatcttcgt ctgaagttct gtaacgagtt ggatcgtcac cagccattgt
     4981 atgaggacca taacgatatg tcatagtttc aattaatgtt ggaccttcac ctgcaactgc
     5041 gcggtcacgt gcttctttag ttgcttgata tacagctaac gcatccatac catcaacttg
     5101 gataccagga atacctacag caattgcttt ttgagctaat gtttcagcag cagtttgctt
     5161 gcttcttggt gttgaaattg catagttatt gttttgaata acgaaaattg caggtgcttt
     5221 ataagctgct gcaaagttaa taccttcgta gaaatcacct tgtgaagaac caccgtcacc
     5281 agtgtaagtg attgcaactg catttttacc acgtttttta agtgcaaatg caacaccagc
     5341 agcttgaatg tattgtgcac cgataataat ttgtgggctc aatgcattaa cgccttcagg
     5401 gaattgattt cctttgaagt gacctcttga gaataagaaa gcttcagtta atggtaaacc
     5461 atgccaaata atttgaggaa catctctgta tcccggtaaa atgtaatctt ctttttctaa
     5521 agcgtattgt gacgctaatt gtgatgcttc ttgaccagca gttggtgcat agaaacctaa
     5581 acgtccttgt ctgtttaatg agatagaacg ttgatcaagg atacgagtcc ataccattct
     5641 ttccattaat tctactaatt gttcatccgt aagatcaggt actaagtctt catttacgac
     5701 gttaccattc tcatccaaaa tttgaaccat ttcaaatttc gattgagtat catttaaaac
     5761 ttttactgca tcgaattggg cttgtaactt aggagccatt caattcacca tacctttccc
     5821 ataattgtat agaaattcat tttatcctta ctaattgtac cacaaataaa tgggactgtt
     5881 aaaccatttg aaaaagttct gttttaataa aaaaagatac agataatagg aactgtatta
     5941 gttgtttcct accatctgta ttatattaat tatttaaatt gatcaacgtc ttgcttttct
     6001 ttaccaacct tatttagtac tttcgtatac ttatctgata cttcttttaa ctttttatag
     6061 ttctgttcta ttgcttttga tttttcgtta acaccttgtt gtgtcgcgtc attttgattt
     6121 aaatatttaa ataatgtttt ctctgagttt acagcctttt tatatgcttt cgcgtattca
     6181 ccatgcaatt tgtatttttc ttttaataca tcatctaatt gttttacttc tttacgttta
     6241 acatcattat caatgttatc aacgtgactt tttgctttct taaagtcttg ttcagacttc
     6301 ttaattgcgt cttcttcttt ttcaaattcc tttagacgat catcggcatt tttaattaaa
     6361 tcttttactg ctttaccgcg aactgctgta tctttactat tgacatcttc agttaatttt
     6421 ttctttttct catctaattc agcaattttc ttaccaacag ttttaattgg ttcttcatcg
     6481 tctttaattt tatccacttg ctttaaatat gccttaattt cttttttatc cgtagtacat
     6541 cctgcaagca agacactaga tgctaatact actgcgattg tttttccaaa tttcattact
     6601 tgtacctcca cttgctattt aaattaaatg ataaataaaa tctgcttgga aatcaatttt
     6661 aaacaattac attacacatc ttcattacta agtatatctg ttgacagtta cgtcatttat
     6721 aatataattt ttaaagtcaa gatgataaat aaatttctaa agtatcattg cttggaacca
     6781 acactttaat cattttttaa gcaattagca tatctgaaaa tttacatata ctatctaaaa
     6841 taaacattgg cagtagttgg ttaactttgt agatatttac acataaaaat gtatgagtat
     6901 cttaacaacc tttttactgt cattcaacta aaacaaatta aaataaggaa ggtgcaatat
     6961 atgttaacaa tgaaagacat cattagagat ggtcatccaa ctttgcgtca aaaagcagct
     7021 gagttagaat taccattaac taaagaagaa aaagaaacat taatcgccat gagagagttt
     7081 ttagtaaata gtcaagatga ggaaatcgcg aaacgatatg gtttacgttc aggcgttggt
     7141 ttggctgcac ctcaaattaa tatttctaaa cgtatgattg ctgttttaat accagatgat
     7201 ggcagtggca aatcttatga ctatatgctt gtgaacccaa aaattgtaag tcatagcgtt
     7261 aaagaagctt atttaccaac tggtgaaggt tgccttagtg tcgatgataa tgttgctggt
     7321 ctagttcacc gtcataatag aattacaatt aaagccaaag acatcgaagg taatgatata
     7381 caattacgac taaaaggata tccagcaatt gttttccaac atgaaattga ccatttaaat
     7441 ggtgtaatgt tctatgatca cattgacaaa aatcacccat tacaaccaca tacagatgct
     7501 gtagaagttt aaagcacatt tttactaatt atcaaagctt aggataaata tgatgtccta
     7561 agctttttct tgtacaactt tttcgaataa acaacagtta aatatattca cctttctacc
     7621 aaacttttta tcccctcatt taaattttac cgtttcatat aaaatccttt aattctttct
     7681 taacattaat ttataatctc tacatttatt ttaataaata gaactgcaca tttattcgaa
     7741 atacttagat ttctagtgag ataaactgct ttatttatta tcattcatca tgtaaaataa
     7801 gatttaactg aaattttagt gttatttcac taatttttta aaatgaacga catgatgaac
     7861 ctagttatta accaaatcgt tattaagtta aattatagag atgattggaa tgaatttatc
     7921 gatatatact ccaatacgat tttactaggg ttaacaataa attaaacaaa cattcttagg
     7981 aggaattttt aacatggcag tatttaaagt tttttatcaa cataacagag acgaggtaat
     8041 tgtgcgtgaa aatacacaat cactttatgt tgaagctcaa acagaagaac aagtacgtcg
     8101 ttacttgaaa gatcgtaatt ttaatatcga atttatcact aaattagagg gcgcacattt
     8161 agattacgaa aaagaaaact cagaacactt taatgtggag attgctaaat aatgaaacaa
     8221 ttacatccaa atgaagtagg tgtatatgca cttggaggtc taggtgaaat cggtaaaaat
     8281 acttatgcag ttgagtataa agacgaaatt gtcattatcg atgcaggtat caaattccct
     8341 gatgataact tattagggat tgattatgtt atacctgact acacatatct agttcaaaac
     8401 caagataaaa ttgttggcct atttataaca catggtcacg aagaccatat aggcggtgtg
     8461 cccttcctat taaaacaact taatatacct atttatggtg gtcctttagc attaggttta
     8521 atccgtaata aacttgaaga acatcattta ttacgtactg ctaaactaaa tgaaatcaat
     8581 gaggacagtg tgattaaatc taagcacttt acgatttctt tctacttaac tacgcatagt
     8641 attcctgaaa cttatggcgt catcgtagat acacctgaag gaaaagtagt tcataccggt
     8701 gactttaaat ttgattttac acctgtaggc aaaccagcaa acattgctaa aatggctcaa
     8761 ttaggcgaag aaggcgttct atgtttactt tcagactcaa caaattcact tgtgcctgat
     8821 tttactttaa gcgaacgtga agttggtcaa aacgtagata agatcttccg taattgtaaa
     8881 ggtcgtatta tatttgctac cttcgcttct aatatttacc gagttcaaca agcagttgaa
     8941 gctgctatca aaaataaccg taaaattgtt acgttcggtc gttcgatgga aaacaatatt
     9001 aaaatcggta tggaacttgg ttatattaaa gcaccacctg aaacatttat tgaacctaat
     9061 aaaattaata ccgtaccgaa gcatgagtta ttgatactat gtactggttc acaaggtgaa
     9121 ccaatggcag cattatctag aattgctaat ggtactcata agcaaattaa aattatacct
     9181 gaagataccg ttgtatttag ttcatcacct atcccaggta atacaaaaag tattaacaga
     9241 actattaatt ccttgtataa agctggtgca gatgttatcc atagcaagat ttctaacatc
     9301 catacttcag ggcatggttc tcaaggtgat caacaattaa tgcttcgatt aatcaagccg
     9361 aaatatttct tacctattca tggtgaatac cgtatgttaa aagcacatgg tgagactggt
     9421 gttgaatgcg gcgttgaaga agataatgtc ttcatctttg atattggaga tgtcttagct
     9481 ttaacacacg attcagcacg taaagctggt cgcattccat ctggtaatgt acttgttgat
     9541 ggtagtggta tcggtgatat cggtaatgtt gtaataagag accgtaagct attatctgaa
     9601 gaaggtttag ttatcgttgt tgttagtatt gattttaata caaataaatt actttctggt
     9661 ccagacatta tttctcgagg atttgtatat atgagggaat caggtcaatt aatttatgat
     9721 gcacaacgca aaatcaaaac tgatgttatt agtaagttaa atcaaaataa agatattcaa
     9781 tggcatcaga ttaaatcttc tatcattgaa acattacaac cttatttatt tgaaaaaaca
     9841 gctagaaaac caatgatttt accagtcatt atgaaggtaa acgaacaaaa agaatcaaac
     9901 aataaataat caaaaagcta ctaactttga agtgaagttt taattaaact caccacccat
     9961 tgttagtagc tttttcttta tatatgatga gcttgagaca taa
//
LOCUS       DAOBBH010000091         9942 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.091, whole genome shotgun sequence.
ACCESSION   DAOBBH010000091 DAOBBH010000000
VERSION     DAOBBH010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9942
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.091"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(40..495)
                     /locus_tag="P6L01_001607"
     CDS             complement(40..495)
                     /locus_tag="P6L01_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HDP3030676.1"
                     /translation="MKNLKNSLFISLIIGLSLSLFFSMLFADGKYYPLNPQSTIGILY
                     YNHFTETTVMLISIILWLLIGVVFFLGDFIFKYTDWSITKATIMHFITTYVGFLPLAM
                     LAGWFPLTVHYLIIFTIIFIVIYVLIWIIQFFKNKNYVDTINEQLKQLK"
     gene            complement(492..935)
                     /locus_tag="P6L01_001608"
     CDS             complement(492..935)
                     /locus_tag="P6L01_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDP3030677.1"
                     /translation="MMKLNLFINANETESYIDIHAPKMNDHVQSIINAVNDLDKSHTL
                     VGYIDKEIHIINVSDVITFQVINKNVTAITSNQKFKLKLRLYELEKQLPQHFIRISKS
                     EIVNKYYIEKLLLEPNGLIRMYLKDAHYTYSSRRYLKSIKERLSI"
     gene            complement(1098..2471)
                     /gene="hssS"
                     /locus_tag="P6L01_001609"
     CDS             complement(1098..2471)
                     /gene="hssS"
                     /locus_tag="P6L01_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme sensor histidine kinase HssS"
                     /protein_id="HDP3030678.1"
                     /translation="MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKI
                     MKTLKEARQYEQSAKPTHIQQYFKHLGQMNYQIMTVDQKGHKTFYGEPFREDTLSQNA
                     INNVLNNKDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTKDGSIAVFMRPDIGETFS
                     EFRTFLAVLLMLLLFISISLVIASTYSIIRPVKKLKLATERLIDGDFETPIKQTRKDE
                     IGTLQYHFNKMRESLGQVDQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDKTLRQQ
                     YINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVDQLIKDIIRHEQFAADEKS
                     LIILADLESINFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSHNNIIFTISND
                     GSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNEYVTTFTI
                     TLPNNSH"
     gene            complement(2464..3138)
                     /gene="hssR"
                     /locus_tag="P6L01_001610"
     CDS             complement(2464..3138)
                     /gene="hssR"
                     /locus_tag="P6L01_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding heme response regulator HssR"
                     /protein_id="HDP3030679.1"
                     /translation="MVQCLVVDDDPRILNYIASHLQTEHIDAYTQPSGEAALKLLEKQ
                     RVDIAVVDIMMDGMDGFQLCNTLKNDYDIPVIMLTARDALSDKERAFISGTDDYVTKP
                     FEVKELIFRIRAVLRRYNINSNSEMTIGNLTLNQSYLELQVSNKTMTLPNKEFQLLFM
                     LAARPKQIFTREQIIEKIWGYDYEGDERTVDVHIKRLRQRLKKLNATLTIETVRGQGY
                     KVENHV"
     gene            3274..4329
                     /locus_tag="P6L01_001611"
     CDS             3274..4329
                     /locus_tag="P6L01_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3030680.1"
                     /translation="MKLAIKEIMFYKFRYILITLIILLLSIMVLFISGLAQGLGRENI
                     SLFEHFDNDEYVVQKMKEPQIEKSQLSDTQQNQIKKVIHQEPYKMNIQTLKLSNKEQD
                     VITMNDVKQQRIQLKKGDYPKNAHEVAINDKLAADNIRVGDRLHFKNNSTSYRVSGIL
                     NDTMYAHSSIVLLNDNGFNALNKVNTAFYPVKNLTQQQRDELNKINDVQVVSEKDLTG
                     NIASYQAEQAPLNMMIVSLFAITAIVLSAFFYVMTIQKISQIGILKAIGIKTRHLLSA
                     LVLQILTLTIIGVGIAVIIIVGLSFMMPVTMPFYLTTQNILLMVGIFILVAILGASLS
                     FIKLFKVDPIEAIGGAE"
     gene            4329..4994
                     /locus_tag="P6L01_001612"
     CDS             4329..4994
                     /locus_tag="P6L01_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3030681.1"
                     /translation="MALVVKDIVKNFGEGLSETKVLKGINFEVEQGEFVILNGASGSG
                     KTTLLTILGGLLSQTSGTVLYNDAPLFDKQHRPSDLRLEDIGFIFQSSHLVPYLKVIE
                     QLTLVGQEAGMTKQQSSTRAIQLLKNIGLEDRLNVYPHQLSGGEKQRVAIMRAFMNNP
                     KIILADEPTASLDADRATKVVEMIRQQIKEQQMIGIMITHDRRLFEYADRVIELEDGK
                     ITD"
     gene            complement(5225..6193)
                     /locus_tag="P6L01_001613"
     CDS             complement(5225..6193)
                     /locus_tag="P6L01_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDP3030682.1"
                     /translation="MLLNLLLIFILIVICPLIFNYTLTLNLTCYILVVISIFLSLIYS
                     IMTYTFPWSMFVTFIIISIIMLFKHRYLFSHTSSRLFIKKLLSFSYKFVLYTSALLFI
                     STIQSPIVKGLSMWLAILCLSLLLTFICYLVWTVSYRHRSYNKEVDIIIVLGAGIFTE
                     FVTPMLAARLDRALDIYQQQASATKIIVSGGQGPDEPISEALAMQRYLIAHGVDKTDI
                     LMESHSINTYTNFLYSKQIINNLYHEAVKIVCVTSQFHVLRALRLAQKLGLSIDGVGS
                     HTPYHFFLHVLIKDYLGVMYQYRLLLTLYCSSSWFICLYAAFIYNS"
     gene            6388..6843
                     /locus_tag="P6L01_001614"
     CDS             6388..6843
                     /locus_tag="P6L01_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDP3030683.1"
                     /translation="MVKHLQDHIQFLEQFINNVNALTAKMLKDLQNEYEISLEQSNVL
                     GMLNKEPLTISEITQRQGVNKAAVSRRIKKLIDAKLVKLDKPNLNIDQRLKFITLTDK
                     GRAYLKERNAIMTDIAQDITNDLNSEDIENVRQVLEVINHRIKTYSNHK"
     gene            7083..8465
                     /locus_tag="P6L01_001615"
     CDS             7083..8465
                     /locus_tag="P6L01_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDP3030684.1"
                     /translation="MKSCPKCGQQAQDDVQICTQCGHKFDSRQALYRKSTDEDIQTNN
                     IKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTK
                     DNKVDSEEAKVYINYIKDEVGLKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRV
                     SKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKMVIAEANKVTPIGNFIP
                     GTYRIPAMKSTENGDFAGYLKFDFRQSNSETVDVTEDFEEANITVTLKGDTKLNDSSK
                     KVTINDREMTFSSSKTYGPYPQNKDITISASGKAKGKTFTTQTKTIKASDLKYNTEIT
                     LNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSS
                     FYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLINENGKQVDKEYELEQGSQD
                     RLQLIKTSEK"
     gene            8496..8624
                     /locus_tag="P6L01_001616"
     CDS             8496..8624
                     /locus_tag="P6L01_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030685.1"
                     /translation="MEYKQHPFKVFTISVNYFEGIVFCEKIYGVLRFISFYTFYKL"
     gene            8734..9942
                     /locus_tag="P6L01_001617"
     CDS             8734..9942
                     /locus_tag="P6L01_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HDP3030686.1"
                     /translation="MNDLTHFNKRSPIFIIILGSLTAIGALSIDMFLPGLPDIRHDFQ
                     TTTSNAQLTLSMFMIGLAFGNLFAGPISDSTGRRKPLIIAMIIFTLASLGIVFVHNIW
                     LMVALRFLQGVTGGAAAVISRAIASDMYSGNELTKFMALLMLVNGIAPVVAPTIGGII
                     LNYSVWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPR
                     FVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYL
                     VDFIDSQKLMRGMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTI
                     AMDESSSGRGSSSSLLGLVQFLFGGVASPLVGVKGEDNPIPYIIIIIATAVILIILQI
                     YNMKVFKTNR"
BASE COUNT         3517 a         1334 c         1761 g         3330 t
ORIGIN      
        1 atgtgataga cattaccgtt ttttagtggt taataaaaat cattttaatt gtttcagttg
       61 ctcgttaata gtgtctacgt agttcttgtt tttaaagaat tgaattatcc aaattaatac
      121 ataaatcaca atgaagataa ttgtgaatat gattagataa tgcactgtta gtggaaacca
      181 accggcaagc attgctaaag gcaagaatcc gacatacgtt gttatgaaat gcattatagt
      241 tgctttagta atgctccaat ctgtgtattt aaagataaaa tctccaagga aaaagacgac
      301 gcctatgagt aaccataaaa tgatagaaat caacattacg gtagtttctg tgaaatgatt
      361 ataatacaaa atgccaatag ttgattgtgg gttcagtgga taatatttgc cgtctgcaaa
      421 taacatacta aagaacagtg aaagggacaa accaatgatt aagctaataa ataatgagtt
      481 tttcaaattt ttcatattga taagcgctcc tttatagatt ttaaataacg tctagaagaa
      541 taggtgtagt gtgcatcttt aagatacata cgtataagtc catttggctc taataataat
      601 ttttcaatgt aatacttgtt gacgatttct gatttggaaa tgcgaatgaa atgttgtggt
      661 aactgttttt ctagttcata aagtcgtaat tttagtttga atttttgatt gcttgtaatg
      721 gctgttacat ttttattaat aacttggaat gttataacat ctgatacatt aatgatatgg
      781 atttctttgt ctatgtatcc gactaatgta tgtgatttgt ctaaatcatt gactgcatta
      841 ataatacttt gaacgtgatc attcatttta ggtgcatgta tatcaatata agattccgtc
      901 tcatttgcat tgataaataa attgagtttc atcataggtt aatgcctcct tcaaaattat
      961 taaaccataa atgaccatcg atatatttaa attttgttga atggtagaaa ttaaatgtta
     1021 agtggctaga aagcgctaat caatataaaa gatacctcct gaaataaaaa cagaaatgtt
     1081 ttttcaggag gtagagatta atgtgaatta tttggcagtg taatagtaaa ggtggttaca
     1141 tactcgttac tttgtgtgaa ttggattgta ccatgatgca attcaatgat ggattttgta
     1201 attgcaagac ctaaaccatt gctattatca tgtttgctca ctttataaaa acgttcaaat
     1261 aaacgtgctt cagcttgtgg actaattggt gaaccatcat tacttattgt gaaaatgata
     1321 ttgttgtgac tatgttgcaa agcgatgtca atggcaccac caacatctgt atacttaata
     1381 gcatttatta ataaattact caatgcttga tgtaacaaac gttgatttcc taggaaattg
     1441 attgattcta ggtcagctaa aatgattaac gacttttcat cagcagcaaa ttgttcatgt
     1501 cgaatgatat ctttaatgag ttgatcgact tgaatcttat catcgaataa taaatgttgg
     1561 tgattatcta attcggataa gagtaacaat tcagttgtta atccgctcag ctgcgttgta
     1621 atggtataaa tatcatttat atattgttgt cttaatgttt tatcagaagt ttgttgtaac
     1681 tcgcttaata aatgatgaat atgtgttaat ggtgttttga tttcatgaga tacattttgt
     1741 acaaaatgct gcctcatttg atcaacttgt cccaatgact ctctcatctt attaaagtga
     1801 tactgtaatg ttccaatttc atctttgcgt gtttgtttga taggtgtttc aaaatcacca
     1861 tcaattaagc gttcggtcgc tagctttaac ttttttactg gacgaatgat tgaataggtt
     1921 gatgcgataa ctaatgaaat cgagataaat aataacaaca ttaacaagac ggctaagaat
     1981 gttctaaact cgctaaatgt ttctccaata tctggacgca tgaatactgc tattgaaccg
     2041 tcttttgtct taaagttaat accaacagta ttatctgtta cattgtcgaa aaatccagtt
     2101 acgaataatg cgaatggttt atctttaata ccatggtaat ctttattatt taatacgttg
     2161 ttaatagcat tttgtgataa agtatcttct ctaaatggct ccccataaaa tgtcttatga
     2221 cctttttgat caacggtcat tatttggtaa ttcatttgcc ctaaatgttt gaaatattgt
     2281 tgaatgtgcg ttggtttagc tgattgttca tattgtcttg cttctttaag tgtcttcatg
     2341 attttcgcgt cattagatgc ttttaaatta taatgatagt aaacatttgt taatacaaaa
     2401 cttattaatg cactaaataa aataaccgta atggaataaa tcgcaattct agcatagagt
     2461 gttttaaaca tgattctcca ccttatagcc ttgtcctctt actgtttcaa ttgtaagtgt
     2521 ggcatttaat ttttttaatc tttggcgtag tcgcttaata tgaacgtcaa ctgttcgttc
     2581 atctccttca taatcatagc cccaaatttt ttctattatt tgttcacgag taaagatttg
     2641 cttaggacgt gctgcaagca taaataataa ttgaaattcc ttgttcggta acgtcatcgt
     2701 tttattagat acttggagtt ccaaatagga ttggtttagc gttaagttgc caatagtcat
     2761 ttctgaattt gaattgatat tatatcgacg taataccgca cgaattctaa aaataagttc
     2821 cttaacctca aagggtttgg ttacataatc gtcagtacca cttataaacg cacgctcttt
     2881 gtcactaagt gcatcccgcg ctgttaacat aataactggt atatcataat cattttttaa
     2941 tgtattacat aattgaaagc cgtccatacc atccatcata atatctacca ctgcaatatc
     3001 gacacgctgt ttttctaaaa gttttaatgc agcttcacca cttggttgtg tgtatgcatc
     3061 aatgtgctct gtttgtaaat ggctagctat ataatttagg attcgaggat cgtcatcgac
     3121 aacaagacat tgcaccatag ctataaactc ccttatcttt ttcatttatt atacatgtaa
     3181 aatatttttg cgtaaaaaaa caattgttca tattgagttc atatttcaac cttatactga
     3241 cgctaaagaa gaaataggga gaagtgaatc gatatgaaat tagcgataaa agagattatg
     3301 ttttacaaat ttcgttatat tttaatcaca ttaatcattc ttttattaag tattatggtg
     3361 ttatttatta gtggtttagc tcaagggctt ggtagggaga atatttcgtt atttgaacac
     3421 tttgataatg atgaatatgt tgttcaaaaa atgaaagagc cgcaaattga gaaatcgcaa
     3481 cttagtgata cgcaacagaa tcaaattaaa aaggtaattc atcaagaacc atataaaatg
     3541 aatattcaaa cattgaaatt atctaataaa gaacaagatg tgattacaat gaatgacgtt
     3601 aaacaacaac gcatacaact taaaaaaggt gattatccta aaaatgctca tgaggtcgct
     3661 attaatgata agttagctgc agacaacatt agagtcgggg atagattaca ttttaaaaat
     3721 aattcaacta gttatagagt ttctggtatt ttaaacgaca caatgtatgc gcatagttcc
     3781 attgtgctat tgaacgataa cggatttaat gcattgaata aggttaatac ggcattttat
     3841 ccagtgaaaa atttaacaca acaacaacgt gatgagctta ataaaataaa tgacgttcaa
     3901 gttgtgagtg aaaaagattt aacaggtaat attgcgagtt atcaagcaga gcaagcaccg
     3961 ttaaatatga tgattgttag tttgtttgct attacagcaa tcgttctaag tgcatttttc
     4021 tatgttatga cgattcaaaa aatatcacaa attggcattt tgaaagcaat tggtattaag
     4081 acaagacatt tattgagtgc gttagtttta caaattttaa cactaacaat aattggggta
     4141 ggtattgctg tgatcatcat agtaggacta tcatttatga tgccggtaac gatgcctttt
     4201 tacttaacaa cgcaaaatat tttattaatg gtggggatat ttatattagt agcgatttta
     4261 ggtgcctcac tatcatttat caaattattt aaagtggatc ctatcgaagc aattggaggt
     4321 gcagaataat ggcattagtc gttaaagata tcgtcaaaaa tttcggagaa ggtttgtctg
     4381 aaacaaaagt tttaaaaggt attaattttg aagtggaaca aggggaattt gtcattttaa
     4441 atggtgcctc tggttctggg aaaacaacat tgctaacgat attaggcgga ttgttaagtc
     4501 aaacgagtgg tacagtgctt tacaatgatg caccattgtt tgataaacag catcgtccta
     4561 gtgatttgcg attggaagat attggtttta tttttcaatc ttcacattta gttccttatt
     4621 taaaagtgat agagcaattg acactcgtag gccaagaagc gggaatgacc aaacaacaaa
     4681 gttcaacaag agcaatacaa cttttgaaaa atattgggtt agaagatcgc ttgaatgtat
     4741 atccgcatca gttatctggc ggtgaaaagc aacgtgttgc gattatgaga gcatttatga
     4801 ataatccgaa aatcatttta gcagatgagc ccacagcaag tttagatgcc gatagagcaa
     4861 caaaagttgt tgagatgata cgtcaacaaa ttaaagaaca acaaatgatt ggtattatga
     4921 ttacacacga tcgaagatta tttgaatatg cagatcgagt gattgaatta gaagatggca
     4981 aaataactga ttagtggctt gtaaagacgc taaatgttaa tgatttaaga catagtagta
     5041 taaaagttag ataacagaat acgatttggg tttacaaaaa agcgcgtcac tattatataa
     5101 aatgatgacg tgctttagtt tatttatttt caatataaca attgtctaat caaggattaa
     5161 cgaatattta aagatagttt gacgcaatat tagaaacaac ctataataat agtttgtttg
     5221 tggattaact attataaata aaagcggcgt aaagacatat aaaccaacta cttgaacaat
     5281 ataacgttaa taacaatcta tactgataca ttacgcctag ataatcttta atgagcacat
     5341 gtaagaaaaa gtgatatggt gtatgacttc cgacaccatc gatagataaa cctaattttt
     5401 gggctagtcg taaggcgcgc aatacatgaa actgacttgt tacacaaaca attttaactg
     5461 cttcatgata caaattgttg atgatttgtt tagaatataa aaagtttgtg tatgtattta
     5521 tagagtgaga ttccattagt atatctgttt tatcaacacc atgtgcaatc aaataacgtt
     5581 gcatagctaa agcttcagaa attggttcgt ctggtccttg tccgccagat acaataatct
     5641 ttgttgctga tgcttgttgt tgatagatat caagtgcacg gtctaaacgc gctgcaagca
     5701 ttggtgtgac aaattcggta aaaataccag cacctaacac aattatgata tcaacttctt
     5761 tgttgtatga tctatgtcta tatgatactg tccaaacgag ataacaaata aaggttagta
     5821 acagggaaag acataatata gctaaccaca tagacaaacc tttcacaata ggtgactgaa
     5881 tcgtacttat aaatagaagt gctgatgtgt agagtacaaa tttatatgaa aaagataata
     5941 attttttaat aaataagcga ctagaagtat gagaaaataa atatctatgt ttgaatagca
     6001 tgataatact gattattata aatgttacaa acatagacca agggaaagta taggtcatga
     6061 tgctatagat gagtgacaaa aatatcgata tgacaactaa gatgtagcat gttaaattta
     6121 acgtcagagt atagttgaaa attaacggac aaataacgat aagtataaat attaataata
     6181 aattcaataa catactgaca cctcgcttat aataaatatt aaatataaat gtagatgatt
     6241 taatttatta aagcaaggag aacgcagcaa catgtaagtc ttaatttgtt atattatata
     6301 tgggtcaata tttttgtgtt ttttagatgt gcgatacaat gattgctgag gatactttat
     6361 aaaaatagta attagggaga aataattatg gtaaaacatt tacaagacca tattcaattt
     6421 ttagagcagt ttataaataa cgttaacgca ttaactgcaa aaatgttgaa agatttacaa
     6481 aatgaatatg aaatttcatt agagcagtct aacgtattag gtatgttaaa taaagaacct
     6541 ttgacaatta gtgaaatcac gcaaagacaa ggagtaaata aggccgcagt aagccgacga
     6601 attaaaaagt taatcgatgc taaattagtt aagttagata aaccaaattt aaatattgat
     6661 caacgtttga aattcataac cttaactgac aaaggtagag catatttgaa agaacgtaat
     6721 gcgattatga cagatattgc gcaagatatt actaatgatt taaattctga agatattgaa
     6781 aatgtaaggc aagtattaga agtcatcaat catcgcatta aaacatatag caatcataaa
     6841 taggtttttg aaggtgttca aatataagac taaatatgta tgtattgaac accttctttt
     6901 tagttataat tgagagagta ctttcataaa taatgtaata aggtacaatt ctaaagtaaa
     6961 gttaatcatt tcttgaattg tattcttggg ataatatttg taaaataata atggtaaaat
     7021 aatgaaataa tttttgtgat atacatataa caattgaaga ggaacagaat tgaggtgaaa
     7081 gtatgaaatc ttgcccgaag tgcggacaac aagcacaaga tgatgtacag atatgtacac
     7141 aatgtggaca taaatttgat agtcgtcaag cattatatag aaagtcaacc gatgaagata
     7201 tacaaactaa taacatcaag atgagaaaaa tggtgccttg ggcaattggt tttttcattt
     7261 taatcttaat tattatattg ttctttttac taagaaactt caattcacct gaagcgcaaa
     7321 ctaaaatatt agtgaatgcg attgagaata atgataaaca aaaagtagca acattattaa
     7381 gtactaaaga taataaagta gattctgaag aagcgaaagt atacattaac tatatcaaag
     7441 atgaagttgg gcttaagcaa tttgtcagcg accttaaaaa tacggtacat aaattgaata
     7501 agagtaagac cagcgtagct tcttatattc aaaccagatc tggtcaaaat atattacgtg
     7561 taagtaaaaa tggcacgcgt tatatctttt tcgataatat gagctttact gcacctacca
     7621 agcaaccaat tgttaagccg aaagaaaaaa caaaatatga gtttaaatct ggtggtaaga
     7681 aaaagatggt tatagctgaa gcaaataaag tgacgccaat aggtaatttt ataccgggga
     7741 catatagaat tccagctatg aaatcaactg agaacggtga ttttgcaggc tatttaaaat
     7801 ttgattttag acaaagtaat tctgaaacgg tagatgttac tgaagatttt gaagaagcaa
     7861 atataactgt aactttaaaa ggcgatacaa aattaaatga tagttctaaa aaagtaacta
     7921 taaatgaccg tgaaatgaca ttttcaagtt ccaaaacgta tggtccatat ccacaaaata
     7981 aagatattac catttcagct tcaggtaaag cgaaaggtaa aacatttaca acacagacga
     8041 agacgattaa agccagcgat ttaaaataca atacagagat aactttgaat tttgacagtg
     8101 aagatatcga agactatgtt gaaaagaaag aaaaagaaga aaacagcttg aagaacaaat
     8161 tgatagaatt ctttgctgga tattctttag cgaataatgc tgcgtttaat cagtcagatt
     8221 ttgattttgt atcgtcatat ataaaaaaag gatcatcttt ttatgatgat gtaaagaagc
     8281 gtgtatctaa aggaagttta atgatgatta gttcaccaca aattatagat gctgaaaaac
     8341 atggtgataa gattaccgca accgtaagat taataaatga aaacggtaag caagtagata
     8401 aagaatatga gcttgaacaa ggctcgcaag accgcttgca attaatcaag acatcagaaa
     8461 aatagagtat aaaacttaaa taagatgcat gtgtcatgga atacaaacaa catcctttca
     8521 aagtatttac aattagtgtg aattactttg aagggattgt tttttgtgaa aaaatttatg
     8581 gagtgctgag gtttatatca ttttatacct tttataagtt gtaatgatgt tcatgaaact
     8641 tgtgagtaat gctaatatat aaaagagcat atttaaattt agtttctgtt cgtgatagtt
     8701 ataaactaat cattatataa aggagatagt catatgaatg atttaacgca ttttaacaag
     8761 cgttcaccga tatttattat tattttagga tctttgacgg ccattggagc attatcgata
     8821 gatatgtttt tgcctggatt accagatatt agacatgatt ttcaaacaac gacgtctaac
     8881 gcacaattaa cattatcgat gtttatgatt ggtttagcgt ttggtaattt gtttgctggt
     8941 ccaatttcag attcaactgg gcgcagaaag ccgcttatta tagctatgat tatttttaca
     9001 ttagcaagtt taggtattgt ttttgtacat aatatttggc tgatggttgc attaagattt
     9061 cttcaaggag tgacaggtgg cgcagcagca gttatttcaa gagctatagc aagtgatatg
     9121 tatagtggta atgaactaac taagtttatg gcattattga tgcttgttaa tggtattgca
     9181 ccagttgttg cgccaacaat agggggcata attttaaatt attctgtctg gcgtatggtc
     9241 tttgtcattc tgactatctt tggtttcgtc atggtcatag gttctttatt aaaagtacct
     9301 gaatcattaa cagtgacaaa tcgtgagtca agtagtggat taaaaacaat gtttaaaaac
     9361 ttcaaaatat tattgaagac gccacgtttt gtattaccaa tgctcattca aggtatgaca
     9421 tttgtaatat tatttactta tatttctgcg tctccgttta ttattcaaaa aatttatggc
     9481 atgactgcaa tacaatttag ttggatgttt gcaggaattg gtattacgct cattatatca
     9541 agtcaactta ctgggtacct tgttgatttt atagactcac aaaaattgat gagaggcatg
     9601 acgatgattc agattatcgg tgtaatttta gtgacgattg tattgcttaa tcattggaac
     9661 ttttggattt tagcgatagg atttatcatt ttaatagcac cagtgacagg tgttgcgaca
     9721 cttggtttta caatagctat ggatgaaagt agtagtggta gagggagttc atcaagttta
     9781 ttaggtttag tgcagttttt atttggtggt gttgcatcgc cattagtggg ggtaaaaggt
     9841 gaagataatc ctatacctta cataattatt attatagcta cagcagttat attaattatt
     9901 ttacagattt ataatatgaa ggtatttaaa acaaatcgat ga
//
LOCUS       DAOBBH010000092         9923 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.092, whole genome shotgun sequence.
ACCESSION   DAOBBH010000092 DAOBBH010000000
VERSION     DAOBBH010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9923
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.092"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(490..1644)
                     /locus_tag="P6L01_001618"
     CDS             complement(490..1644)
                     /locus_tag="P6L01_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDP3030687.1"
                     /translation="MKLSLNSNSKYLRAPSIRQFSNRMNNLDDCVNLTIGQPDFPMPD
                     VVKKAYIDAINNDKTSYSHNKGLLETREAISQYFKNRYHFSYDPEEIIVTNGASEAID
                     TTLRSIIEPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKITPDALESHISPK
                     TKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPIFIISDEIYAENTFSGKHVSFAEFE
                     DIRDQLILIGGLSKSHSATGIRIGFLLGPQYLIDKLTFMHAYNCICANVPAQIACITA
                     LNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPSIKHITDDDFEFC
                     VDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMKRLAKYLNTK"
     gene            complement(1840..2850)
                     /locus_tag="P6L01_001619"
     CDS             complement(1840..2850)
                     /locus_tag="P6L01_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDP3030688.1"
                     /translation="MTSLKERDYFFDNARAILILLVVFGHMLQPYTSGDKYLSALYLV
                     IYSFHMPTFLFISGYFAKNIDKPYYLEKISKRLIVPYMIFFAFFSIYYFLTGKSDELQ
                     LDPFNPVFALWFLITLFFFHVILVIVRRFNPYKVLSVSIIISIGAGFSDNIDSYLSIS
                     RTIVFFPIFYLGYIFTKKHTAIFKNKKLIPVSIITFILFFIIYVIHPINADWLLGSSP
                     YTSLENEGQSIFSPFKRLILYGIILIAMTAFLNLMSTKKKLYTYIGSRTLYVYLLHGL
                     IIGIVRGFEWYPFDNPISLMTYLYLISISVIIVYVLSTNFVCKWTNPIINLQRPSQFK
                     DS"
     gene            3003..3422
                     /locus_tag="P6L01_001620"
     CDS             3003..3422
                     /locus_tag="P6L01_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDP3030689.1"
                     /translation="MYKQLEKLITLTNNDLNLVNRRFGQRTDITSEQLELLRILFNYD
                     RLSQYDLTMKISREQSIVSRWIKKLVLKGYITSQQSSEDLRCKELILTDQARTLISQI
                     NNARCELIEARCQCLSEVELDNLNQLLDKLNQRRISL"
     gene            3449..3559
                     /locus_tag="P6L01_001621"
     CDS             3449..3559
                     /locus_tag="P6L01_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030690.1"
                     /translation="MSLCTQQKLNHELQAKVAVIVKIDGKQSPLFLLVYV"
     gene            complement(3629..7375)
                     /locus_tag="P6L01_001622"
     CDS             complement(3629..7375)
                     /locus_tag="P6L01_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional autolysin"
                     /protein_id="HDP3030691.1"
                     /translation="MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLD
                     SNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVN
                     GDTRANQSATTNNTQPVAKSTSTTAPKTNTNVTNAGYSLVDDEDDNSEHQINPELIKS
                     AAKPAALETQYKAAAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYKPQVNSSIN
                     DYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTINGEISYMKNN
                     YQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASFARSMNNYA
                     DYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSHNYSYDQLY
                     DLINEKYLIKMGKVAPWGTQFTTTPTTPSKPTTPSKPSTGKLTVAANNGVAQIKPTNS
                     GLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVKEGDVVYNT
                     AKSPVNVNQSYSIKSGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQIDKSIYLYG
                     SVNGKSGWVSKAYLVDTAKPTPTPIPKPSTPTTNNKLTVSSLNGVAQINAKNNGLFTT
                     VYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVIYNNAKSPV
                     NVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIYLFGTVNGK
                     SGWVSKAYLAVPAAPKKAVAQPKTAVKAYTVTKPQTTQTVSKIAQVKPNNTGIRASVY
                     EKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNVQNLGKEVK
                     TTQKYTVNKSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDVYLYGTINNR
                     TGWVNAKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYSLKAFNEQP
                     FAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVAQIQAGLQY
                     KPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISIDKINQFLKG
                     KGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNKVVTNSNTK
                     YHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQNTLYKMRW
                     NPAHPGTHQYATDVDWANINAKIIKGYYDKIGEVGKYFDIPQYK"
     gene            complement(7603..8037)
                     /locus_tag="P6L01_001623"
     CDS             complement(7603..8037)
                     /locus_tag="P6L01_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDP3030692.1"
                     /translation="MFSKVNNQKMLEDCFYIRKKVFVEEQGVPEESEIDEYESESIHL
                     IGYDNGQPVATARIRPINETTVKIERVAVMKSHRGQGMGRMLMQAVESLAKDEGFYVA
                     TMNAQCHAIPFYESLNFKMRGNIFLEEGIEHIEMTKKLTSLN"
     gene            complement(8192..8662)
                     /locus_tag="P6L01_001624"
     CDS             complement(8192..8662)
                     /locus_tag="P6L01_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499548.1"
                     /note="Essential protein for resistance to osmotic stress
                     from salt; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic stress response protein"
                     /protein_id="HDP3030693.1"
                     /translation="MSRKTYEKIANINGMFNMLEQQIIHSQDMAHFRSEFFYVNHEHR
                     ENYEALLIYYKNSIDNPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMK
                     SLSIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL"
     gene            complement(8710..>9923)
                     /locus_tag="P6L01_001625"
     CDS             complement(8710..>9923)
                     /locus_tag="P6L01_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase"
                     /protein_id="HDP3030694.1"
                     /translation="KFLKYFLILLALVLIVVPIVFATLLFKTSQDAFESSQDSKNANR
                     QSNLRDNKVNPEEQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQIRM
                     LSIPRDTISYIPKVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFVEA
                     VNELGGIYYDVPYDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRGQR
                     QMELIKILFQKAQEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIKTA
                     QLKGKDDMLNGIYYYHPSVESIQKYANLLRKDLELSPINDKNDFLDQRVINHYGSLIP
                     LTPLDSSLLRKEQNDTTDKDKTSNENNDSTNNSDSSNQQQPATDQNLNQNQGGTQQAP
                     QASNNQNGVVN"
BASE COUNT         3131 a         1538 c         1606 g         3648 t
ORIGIN      
        1 acactaaaaa tttttaagtc aataagaata tataaataaa ttaaaaagtt tttgtaaaga
       61 ttagagttta caaaaaacat taagggaatt aaaattgaat tgtattatac tttattcaat
      121 tgagttaaat tgataagatc gtcaattgtt tgcatttatc taaaagaata aataagaaag
      181 gaattgagag cgaatggaaa gtatttgttt ataataataa atagtaactt ttgaataata
      241 attgtttggc caaaggtaga ttaatatgaa tggtgatata ttatttgagt gcttaagttt
      301 cgaagaacaa ttataagtta gcatattaaa accttttgat agaatcattt tgagtgaggt
      361 gtttaaagga gaggaatata actcggtagt atttacagtc tctatagagt attatttaaa
      421 acttatagaa tataaaaaac tgaacgtact atgaatttag ttcacgacgt ccagttcaac
      481 atatcatttt tatttagtat ttaaatattt agcaagtctc ttcatacctt cttttaaaac
      541 atccatttca taagcatagg aaatccttac aaatccttta ccgaattctg taaaggatga
      601 gcctggaacg attgctaaat gtgttgattc aagtaaatcg acacaaaatt cgaaatcgtc
      661 atcggtgata tgtttaatac ttgggaaaat ataaaacgcg ccttcaggtt gagcggtaat
      721 ctcaaaacct aatttagtta attcagatac taaataattt cttcgttcta cataagcttc
      781 gttcatatat ttaggagctt ctaatccttc gttaagtgct gttatacatg ctatttgagc
      841 tggaacattg gcacaaatac aattataggc gtgcataaat gttaatttat caatcaaata
      901 ttgaggccct aatagaaaac caattcttat tccggttgct gagtgtgatt tacttaaacc
      961 accaattaat attaattgat cacgaatgtc ttcaaattca gcgaaggata catgtttacc
     1021 actaaatgta ttttcagcat aaatctcatc gctaataata aatatcggat atttttttaa
     1081 tacatttacg atatttaaaa cttcatttct ttttaaaact acgccagttg gattagtcgg
     1141 ataatttaac aagacagctt ttgtctttgg agaaatatga ctttctaatg catcaggtgt
     1201 aattttgaat tgtgttgctg ttgtatcaat ataaattggt ttaccaccta gtacttcgat
     1261 gagtggtatg tagcctgcat aaattggtcc cggtattata atttcatctc caggctctat
     1321 gatagaacgt aacgttgtgt ctattgcttc acttgctcca tttgtcacaa taatttcttc
     1381 aggatcatag gaaaaatgat aacgattttt gaagtattga ctaattgctt cgcgagtttc
     1441 taataaccct ttattgtgag agtatgatgt cttgtcattg ttaatagcat caatatatgc
     1501 ctttttcaca acatcaggca tcgggaagtc tggttggcca atagttaaat taacacaatc
     1561 atccaaatta ttcatacgat ttgaaaattg gcgaatactt ggtgctctta aatatttaga
     1621 attagaattt aaagaaagtt tcatttgtga cacctcaaat ataaatcaaa tattgtctaa
     1681 aaatttagaa attaataata tcataacata cttttaaacc aaaaaagggt aaattactta
     1741 agctttattt gaaaaacgaa aaatgtctaa atacactgta gtaactactt ttcaaaatga
     1801 ataaagtggt tacagttaat gtacttagac actataaaat tatgaatctt taaactgtga
     1861 tggtctttgt aaattaatta ttggatttgt ccatttacaa acaaagtttg tagataatac
     1921 atatacgatg attacagata tacttattaa ataaagataa gtcattaatg atataggatt
     1981 atcgaatggg taccattcaa atcctctaac aatgccaata attaaaccat gtaataaata
     2041 tacgtatagc gtacgactac caatataagt atataatttt ttctttgttg acattaaatt
     2101 tagaaacgca gtcattgcga ttaatataat tccatataat ataagtcgtt taaaaggact
     2161 gaatatactc tgtccttcat tttcaagtga agtatatggt gaacttccca ataaccaatc
     2221 tgcattgata ggatgaatca cgtaaatgat aaaaaacaaa ataaaggtaa tgatagatac
     2281 tggtattagt tttttatttt taaaaatagc cgtatgtttt ttggtgaaaa tgtaacctag
     2341 gtaaaatatt gggaaaaata cgattgtcct tgaaatgctc aagtagctat cgatgttatc
     2401 tgaaaaacct gctccaatag atataataat tgaaactgat agcactttat atggattaaa
     2461 tcttctaact attactaaaa tgacatgaaa gaaaaatagc gtgatcaaaa accataacgc
     2521 aaatactggg ttaaaaggat caagttgtaa ttcgtcactt ttacctgtta agaaataata
     2581 tattgaaaag aatgcaaaaa atatcatata aggtactatc aaacgttttg aaattttttc
     2641 taaatagtat ggtttatcaa tatttttcgc gaaataacca gatataaaca aaaatgttgg
     2701 catatggaaa ctataaataa ctaggtataa tgctgataaa tatttatcgc cactagtgta
     2761 gggttgtaac atatgtccaa atacgactaa taatattaaa attgctcttg cgttgtcaaa
     2821 aaaataatct ctctctttta atgaagtcat attttatctc ctttatgtcc ataagactct
     2881 tactaattta ttatattttg tattagttat gcaacaaatt tgtctattgt gtcgaatagt
     2941 attgtaaaaa ttataaaaaa agcatataat attataaata atatgtagtg gagtagatca
     3001 acatgtataa acaacttgaa aaacttatta cactgactaa caatgactta aacttagtga
     3061 atagaagatt tggacaacgc acggatatca catctgaaca gctagaactt ctccgtattt
     3121 tatttaatta cgatcgctta tcacagtatg atttgacgat gaagattagc agggaacaat
     3181 ctatagtttc aaggtggatt aagaaattag ttttgaaagg ttacatcaca agtcaacaat
     3241 ctagcgaaga tttaagatgt aaagaattaa ttttgactga tcaagcacgt acattaattt
     3301 cacaaataaa taatgcacgt tgcgaattga ttgaagcaag atgtcaatgt ttatcggaag
     3361 tcgaattaga caatttaaat caattacttg ataagttaaa tcaacgacgc atatcgttgt
     3421 aacacgacaa tagcaaacac ataatttagt gtcattgtgc acacaacaaa aattgaatca
     3481 tgaattacaa gcaaaagtag cggtgattgt taaaattgat ggtaaacaat caccgctatt
     3541 tttgcttgtg tatgtataaa aaagggatca aaggtcatcc cccatgattg atagtggggg
     3601 atgacttttg atcctatgtt catgttgctt atttatattg tgggatgtcg aagtatttgc
     3661 cgacttcgcc aattttatca tagtagcctt tgatgatttt agcattgatg ttagcccaat
     3721 ctacatctgt agcatattgg tgtgttcctg gatgtgcagg attccatctc attttgtaaa
     3781 gtgtattttg accagctttt acatatgagt tgccgatgaa tttagcacca ccaacgattg
     3841 cttttgatac tgtgtcccaa ccagcttgtt tagcatattt aataccttca cgtaaaggat
     3901 cgttatcata tgcagcaata ccaaatacgt tatggtattt cgtgtttgag ttagttacaa
     3961 ctttgttgtt cactacatct gcacctttcg ctaattgaga agtaccgtta cctgtttcta
     4021 atagggcatg tgagataaga taaacttcat taatgccata catttgagca gctttgttaa
     4081 atgcagcacc ttggttttct aatacacctt taccttttaa gaattgatta attttatcaa
     4141 tagaaatatt ttgtggttgg tctaagcgta agaattgata ttttaatgct ggatcttgag
     4201 ctaaacgctt cgtatccatt gcatgcttaa catcattaaa gttagcatct gtccacttac
     4261 ctggtacacg ttgtacttgt ggtttatatt gtaaaccagc ttgtatttga gcaacttggt
     4321 ttaatgtcat acctgtttga ttatacttaa ttaattcttt agctaaatca gttgatttaa
     4381 tccatgctaa tttaccgtta gataatttac catagtacca agtttgtcca ttaatgactt
     4441 gttctttaac aactgcgaat ggttgttcat taaatgcttt taatgagtat ttagctgtat
     4501 cagaatttgg tgttacatag taataaccat taccattttt aattacataa gtgtagttat
     4561 aatctttggc agctgatgta gttggtttca cagcagttgg tgcagttaaa tcttttgcat
     4621 ttacccaacc agtgcggtta ttaatagtac cgtataaata aacatctttg cctacagata
     4681 cttgtttcgt tgcattaaat gtaccttgag caatgttatt gcctgttaaa atgacttggt
     4741 ttttagtacc ccaaggaacc attgataagc cgttatttga tttattaaca gtatattttt
     4801 gagtcgtttt aacttctttg cctaggtttt gaacatttaa gtcttttaca ttgaaccaac
     4861 ctaatgggat gttatggctt gtattgttta ataatacata cgtttcatta ccatgagcac
     4921 gctcttttgt tacatagaac gtacggtctg catatttcgc accgtttttc gctgtttttt
     4981 cataaacaga agcacgaata ccagtgttgt ttggtttaac ttgagcaatc ttgctaactg
     5041 tttgagtcgt ttgtggttta gtaacagtat aagcttttac agctgttttt ggttgtgcta
     5101 ctgctttttt aggtgcagca ggtacagcta aatatgcttt acttacccaa ccagatttac
     5161 catttacagt tccaaataaa tagatagatt tatcaatttg ttgttgctta gtcgctttaa
     5221 aagtttggtt acctgtacca gaaactgcac cagcttcttg tttataagtg ccccaaggta
     5281 ctgaatataa tttagtgcct ggttttactg tatatgtttg cattacattt acaggtgatt
     5341 ttgcattgtt ataaataacg tcaccttgtt taacccaacc aattaaagtt ggactattgt
     5401 aatctttaac taagtagaat ttgtttccac ctaaacttgc ttcttttgtt acagcaaatg
     5461 ttttttgaac ttctttcgtt ggcttaccag ttttgtcata aactgtagtg aataagccat
     5521 tgtttttagc attaatttga gcaacaccgt ttaatgatga aactgttaat ttattatttg
     5581 ttgtaggtgt tgatggctta ggtattggtg taggcgtagg tttagcagta tcaactaaat
     5641 atgctttact tacccaacca gatttaccat tcacagagcc atataaataa attgatttat
     5701 caatttgttg ttgctttgaa gccttaaatg tttggtttcc agagccagac acgctaccag
     5761 caacttgttt agatgtaccc caaggtactg tataaagttt cgtaccagat ttgattgaat
     5821 atgattgatt tacatttaca ggtgatttag ctgtgttgta aaccacatcg ccttctttaa
     5881 cccaaccaaa tttattacca gaattgtaat cttgaacaag atagaatttt tgattaccta
     5941 atgtagctgt tttagataca gcaaatgttt tttgaacttc attagttgct ttaccagttt
     6001 tgtcgtaaac agtagtatat aaaccactat ttgttggttt gatttgtgcg acaccattgt
     6061 ttgctgcaac tgttaattta ccagttgatg gtttcgacgg tgttgttggt tttgatggtg
     6121 tagtaggggt agttgtaaat tgcgtacccc atggcgccac tttacccatt tttattaaat
     6181 atttttcatt aattaagtca tataattgat cataactata attatgactt cttaaatatc
     6241 catgtggatc ggcatggtcc gtaccaccta aatatttact tacagcgtag tgagtccata
     6301 ctgtaccatt tccatcatac tcagcactgt ctggttttaa accataatat tgtaattgtg
     6361 tagctgcata gtcagcatag ttattcattg aacgtgcaaa tgaagcatag tcgtgtgtgt
     6421 gtacgatttc aacattgatg aatctagggt taccgactgc accgacaccc caagataagt
     6481 aatccgttgg tgctgtttcg attatacgat ccccatcaac aaatgcatgt acgaatgcgt
     6541 tttgatagtt atttttcata taactaattt caccatttat cgtcgaacga tcattagctg
     6601 tatcatgaac tacgatacct tcaggacgac ctacgccgtt acggtatgcg tatttaggga
     6661 agtaagatgt ataatcttct tcaattttag gtgcttttaa gttattttta cgaatgtaat
     6721 cgttaattga agagtttact tgtggtttat attttggcaa actcgttttt ggtgttgcag
     6781 caactgatct tggttgtgct gaagcgctaa aagtagttac tttaggtgtc gcttcagttt
     6841 tagctttagg tgctgcggct ttatattgcg tttcaagagc tgcaggttta gcagctgatt
     6901 taattaattc tggattaatt tgatgttctg aattatcatc ttcatcatca actaaactat
     6961 aaccagcatt tgtaacatta gtgttagttt taggtgctgt agtgcttgtt gactttgcaa
     7021 caggctgcgt attatttgta gtcgctgatt gattagcacg agtgtcacca tttacttgtg
     7081 cagtatcaac tttttgactt acttgagcat tgcctgtttt gttatttgct gtttttggtt
     7141 ggacaatagc agggtcttga tatacttgag tgccagaaat gttttgcgtt ggatttttta
     7201 cctcagcttt tgcttgttca gtagttgctt taactttatt actatctaaa acatttttat
     7261 tagtagtttg atcttgtgtc gtctcagctg cttgaacttg atgtgcagtg actgctgaac
     7321 ctacaagcgt taatgcaacc attgatggta gtttgtaatt gaattttttc gccattctat
     7381 ttattactcc taacatttat taattattac taacattata gtacctgttt tatatacctg
     7441 tgcgtattta accaatttgt aatatactct taatttacat tttcactgta aaaaaatgaa
     7501 aaaaattttg aatctcaatg gtgacgtgac aaatcaaata gaattgatgt caatttaaga
     7561 attggtttat tacaaatgtt tctaaaatag atacaacttt ttttaattaa gcgaggttaa
     7621 cttttttgtc atttcaatat gctcgatgcc ttcctcaaga aatatattac ctctcatttt
     7681 aaagtttaaa ctttcataaa atgggatagc atgacattgg gcattcatag tagctacgta
     7741 aaaaccttca tctttagcta atgattctac agcttgcata agcattctac ccattccttg
     7801 tccacgatgt gatttcatca cagctactcg ttctattttg acagttgttt cattaatagg
     7861 gcgtattcga gcagtggcaa ctggctgtcc attatcatat ccaatgaggt gaatagattc
     7921 agattcatat tcatcaattt cactttcctc agggacgcct tgttcttcta caaacacttt
     7981 ctttcttata tagaagcaat cttctaacat cttttgattg tttacttttg aaaacataaa
     8041 attcctccac atcctaacaa aatcatttac atttaattta ctaataataa catagaataa
     8101 cgattgttat tttcctcaaa aataattaga cataaaaaag ctataaagat gattcacaag
     8161 aatcatcttt atagctaatt aattattaat gttatagtgc ttcttttcta ataattgctg
     8221 tatattgcca aaagattcgc atttgagcaa tattccaatt attcattccc attgtaaatc
     8281 ctgaaggctt aaatatattc acatcagtta cttctaatgc tgctgcaact aaatattgta
     8341 atggaatgct tagtgatttc attgtttcgg taatgccatt ttcatcatat acccatgaaa
     8401 atggtaactc tgattcgaaa acatcaacac tattgaaaat ttcaggtagg gctaaaatat
     8461 aacatgcacc atctacaata ggattgtcga tactattttt gtaataaatt aggagtgctt
     8521 catagttttc tcgatgctca tgattgacgt aaaaaaattc acttctaaaa tgagccatat
     8581 cttggctatg aatgatttgt tgttctaaca tattaaacat gccatttata tttgcaatct
     8641 tttcgtatgt ttttctactc atgtatgatg ctcctttcat aaatagtaaa tgttaatcgt
     8701 catataatat taatttacaa caccattttg gttatttgaa gcttgtggcg cttgttgtgt
     8761 gccaccttga ttttgattta agttttgatc tgtagcaggt tgttgttgat tgctggaatc
     8821 actgttatta gttgaatcat tgttctcgtt agatgtctta tctttatctg tcgtatcatt
     8881 ttgttctttt ctcaataaac tactatctaa aggcgttaac ggtattaatg aaccataatg
     8941 attaatgaca cgttgatcta agaaatcatt tttatcatta ataggtgata attctaagtc
     9001 tttacgaagt aagtttgcat atttttgaat gctttcaaca cttggatgat aatagtaaat
     9061 accatttaac atatcatctt taccttttaa ttgcgcagtt ttaatttcaa catcatttgt
     9121 taagtacatt tttgctaaag ctttaatttc agagttagtt aaattatgct ttgcattttt
     9181 gcctacaatt tgaatcacgt tatcaagttt atcaatagaa tcaacttcct gtgctttttg
     9241 gaataaaatc ttaattaatt ccatttgacg ttgtccacgt tttaagtctg aatcatgatg
     9301 tctagttcta gcaactgcta aagcctcatc accatttaat ttttggtacc cttttttaat
     9361 tttaatctta ccagtatcat ctgtgttagg ttcatttaag tcgtatggca catcatagta
     9421 tatgccacca agctcattta cagcctcgac aaatgctttc atattgactc tcacataata
     9481 atcaacaggt acattcatgg tagcttctac cgaatccatt gcggcaattg gaccaccata
     9541 tgcatgtgca tgggtaatct tatcgtaata gccaacttta ggaatgtagc tgatagtatc
     9601 acgtggaata ctaagcattc taatttgatg ttttgattga ttaaaagtag ttaaaatcat
     9661 agcgtctgat ctagagtgtt cagcatcctg tccttttttt cttcttccat cgttatcatc
     9721 gatacctaag aaaagaatag agataggttg ttcttcggga ttgactttat tatctcttaa
     9781 gttggattga cgattagcat ttttgctgtc ttgagaagat tcgaatgcat cttgggacgt
     9841 tttaaaaagt aacgtagcga agactattgg aacaacaatg agaaccaatg ctagaaggat
     9901 caaaaagtat tttaaaaatt tat
//
LOCUS       DAOBBH010000093         9852 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.093, whole genome shotgun sequence.
ACCESSION   DAOBBH010000093 DAOBBH010000000
VERSION     DAOBBH010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9852
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.093"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            318..890
                     /locus_tag="P6L01_001626"
     CDS             318..890
                     /locus_tag="P6L01_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase component 1"
                     /protein_id="HDP3030695.1"
                     /translation="METTVSKLERQIEERLKGVSEYESININHRLGKLLDSYDIPDVA
                     KVACLTIDTSMRHLDDITYNHLSKHSILIGDLISAHFYTLLAEINDLSFQNEISKAIV
                     EINELKSSLHHQALNDYEISQAIVKIETLFPYITLSHFGINIDESEIYNYLFEDMSDY
                     YPSYFKKYNQSEVKHYLHDIQKSYLKSRGN"
     gene            893..1618
                     /locus_tag="P6L01_001627"
     CDS             893..1618
                     /locus_tag="P6L01_001627"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HDP3030696.1"
                     /translation="MADNKANKEQVHRVFQNISKKYDRLNNIISFEQHKVWRKRVMKD
                     MGVRKGTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLEVGKEKTASMENVKL
                     VHGDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVVCLETSQPTLP
                     VFKQMYALYFKFVMPIFGKLFAKSKEEYEWLQQSTFNFPGKEELKRMFEEAGFINVRV
                     RSFTGGVAAMHLGYKEKDNTKGD"
     gene            1620..2579
                     /locus_tag="P6L01_001628"
     CDS             1620..2579
                     /locus_tag="P6L01_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDP3030697.1"
                     /translation="MAKLNMNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVR
                     PAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWD
                     QTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIIN
                     YLRRINRKTALLIQISTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTE
                     KKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSERKEFEECIQIIRKSDS
                     IDEAKAVSSKYLSKALNLISELPDGHPKSLLLSLTKKMGSRNT"
     gene            2671..3120
                     /gene="ndk"
                     /locus_tag="P6L01_001629"
     CDS             2671..3120
                     /gene="ndk"
                     /locus_tag="P6L01_001629"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDP3030698.1"
                     /translation="MERTFLMIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMEL
                     AETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIR
                     GDLGLTVGRNIIHGSDSLESAEREINLWFNENEITSYASPRDAWLYE"
     gene            3329..3529
                     /locus_tag="P6L01_001630"
     CDS             3329..3529
                     /locus_tag="P6L01_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030699.1"
                     /translation="MKNIISIILGILMFLKLMELLYGAIFLDKPLNPITKIIFILTLI
                     YIFYVLVKELIIFLKSKYNKSA"
     gene            3915..5081
                     /gene="aroC"
                     /locus_tag="P6L01_001631"
     CDS             3915..5081
                     /gene="aroC"
                     /locus_tag="P6L01_001631"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDP3030700.1"
                     /translation="MRYLTSGESHGPQLTVIVEGIPANLEIKVEDINKEMFKRQGGYG
                     RGRRMQIEKDTVEIVSGVRNGYTLGSPITMVVTNDDFTHWRKIMGAAPISEEERENMK
                     RTITKPRPGHADLVGGMKYNHRDLRNVLERSSARETAARVAVGALCKVLLQQLDIDIY
                     SRVVEIGGIKDKDFYDSETFKANLDRNDVRVIDDSIAQAMRDKIDEAKNEGDSIGGVV
                     QVVVENMPVGVGSYVHYDRKLDGKIAQGVVSINAFKGVSFGEGFKAAEKPGSEIQDEI
                     LYNSEIGYYRGSNHLGGLEGGMSNGMPIIVNGVMKPIPTLYKPLNSVDINTKEDFKAT
                     IERSDSCAVPAASIVCEHVVAFEIAKALLEEFQSNHIEQLQQQIADRRQLNVEF"
     gene            5107..6171
                     /locus_tag="P6L01_001632"
     CDS             5107..6171
                     /locus_tag="P6L01_001632"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDP3030701.1"
                     /translation="MKLQTTYPSNNYPIFVEHGAIDHISTYIDQFDQSFILIDEHVNQ
                     YFADKFDDILSYENVHKVIIPAGEKTKTFEQYQETLEYILSHHVTRNTAIIAVGGGAT
                     GDFAGFVAATLLRGVHFIQVPTTILAHDSSVGGKVGINSKQGKNLIGAFYRPTAVIYD
                     LDFLKTLPFEQILSGYAEVYKHALLNGESTTQEIEQHFKDREILQSLNGMDKYIAKGI
                     ETKLDIVVADEKEQGVRKFLNLGHTFGHAVEYNHKIAHGHAVMIGIIYQFIVANILFN
                     SNHDIQHYINYLTKLGYPLETITDIDFETIYQYMLSDKKNDKQGVQMVLIKHFGDIVV
                     QHIDQTTLQHACEQLKTYFK"
     gene            6181..7479
                     /gene="aroA"
                     /locus_tag="P6L01_001633"
     CDS             6181..7479
                     /gene="aroA"
                     /locus_tag="P6L01_001633"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDP3030702.1"
                     /translation="MVSEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGTSNIYK
                     PLLGEDCRRTMDIFRLLGVDIKEDEDKLVVNSPGYKAFKTPHQVLYTGNSGTTTRLLA
                     GLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGIN
                     YQMEVASAQVKSAILFASLFSNDTTVIKELDVSRNHTETMFRHFNIPIEAERLSITTT
                     PDAIQHIKPADFHVPGDISSAAFFIVAALITPESDVTIHNVGINPTRSGIIDIVEKMG
                     GNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELPVIALLCTQAVGTST
                     IKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIG
                     MMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG"
     gene            7486..8730
                     /locus_tag="P6L01_001634"
     CDS             7486..8730
                     /locus_tag="P6L01_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030703.1"
                     /translation="MEDIYKLIDDINLQKLENLDSRVNEAITTDNDDALFILGETLYN
                     FGLMPQGLEVFRVLYHKYPDESELLIYFIEGLMSENQTDEALEYLSYVEPSPEKLMLE
                     ADLYQQINMMEVAIDKLQEALELEPNDPIIHFALAEMLYYDGQYLRATSEYETVLETG
                     EYQVNGVNLFSRMADCSLQSGNYSDAIRLYDEINEDEMTSEDYLKKAISYDKNDITQE
                     AIKIMTTLLSKDPDYIQGYLYLQSLYENEKNYPDAIETGKEGLRLSQFYKELMYTTGC
                     LEIEHGDANEGVLLLKQALEVDNAYQEPLLILSDLYRNEEDYEAIIELLTYVDEEDLD
                     PTFMWHLAFAYGQEERDKEAQHFFELAYPTMKTNIDFMSDYYFYLIEIGQKEQAITIL
                     NQLLELEPSNENWHDELSRLQS"
     gene            8744..9319
                     /locus_tag="P6L01_001635"
     CDS             8744..9319
                     /locus_tag="P6L01_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDP3030704.1"
                     /translation="MSETLNQIKESFIEYLLFQYRFKSRIAVWVLNYIKVNEAKLANI
                     HFVDTKINHHETLEIAEVGSHASAIQFTKRNIKLMNTNEIFDYIANHNCAFDIQIHFA
                     NVSKREQRLDDLIVAQLTESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMN
                     EKQRFYQLTQILNTLKLVNKHNQFEDLADDD"
     gene            9309..>9852
                     /locus_tag="P6L01_001636"
     CDS             9309..>9852
                     /locus_tag="P6L01_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HDP3030705.1"
                     /translation="MTINTFWQYTLYQRSWLMMLLICNILGMIYGYIWYGEQLSHTPW
                     HFKIFVPDSPTAILYLVISISLILIQKQNSIIEALAFVTLFKYGIWAVIMNILFIIEQ
                     GDITVNGLVLMFSHSIMAVQAIYFYPRFKRSMIGISVAMIWVFLNDYIDYFHLQFPYY
                     DFITTHVWQIGVLSCCLSVFG"
BASE COUNT         3578 a         1312 c         1815 g         3147 t
ORIGIN      
        1 aagcgttcaa aatattacat gacataatag ttttgataaa aatagcaact acaaaatata
       61 catatgaata atgagattaa ttgttagcgt tgaatttact taaaaggtaa ccatgtctac
      121 tatagtattt tacgttattt aaaaagatga ataatgtaaa tgaagtaaag gttattatga
      181 gaattacaaa agctacataa attactgtta gtttaaattg aaatttaaaa atgataagta
      241 atcttaatgc ttcaaaagat tttaatttct tacatataat gttaatatga atagtgataa
      301 tatcgtgagg tgtagacatg gaaacaactg ttagcaaatt ggaaagacaa atagaagaaa
      361 gattaaaggg tgtatcagaa tatgaatcca ttaatattaa tcatcggtta ggtaagttac
      421 ttgattcata tgatattcca gatgttgcca aagtagcatg cctcacaatt gacacatcaa
      481 tgagacacct tgatgacatt acatataatc atttgtctaa acattccatt ttgattggag
      541 atttaattag tgcacatttt tatacgctac tagcagaaat caatgattta tcatttcaaa
      601 atgaaattag taaagcaatt gttgaaatta atgaattgaa atcttcatta catcatcaag
      661 ctttaaatga ttatgaaatt tctcaagcaa tagtaaaaat tgaaacactt ttcccatata
      721 ttacgttatc tcatttcggt atcaatattg atgagtcgga aatatataat tatttatttg
      781 aagatatgtc tgattactat ccatcatact tcaagaaata taatcaaagt gaagtaaagc
      841 attacttaca cgatatccaa aaaagttatt taaaaagtag aggtaattaa gcatggctga
      901 caataaagca aataaagagc aagtacatcg cgtttttcaa aatatttcaa aaaaatatga
      961 tagattaaat aatattatta gttttgagca gcataaagta tggagaaaac gcgtcatgaa
     1021 agacatgggt gttagaaaag ggacgaaagc attagatgtt tgttgtggta ctggtgattg
     1081 gacaatcgca ttaagcaaag ctgtaggacc aacaggtgaa gttactggta ttgactttag
     1141 tgagaatatg ttagaagtag gaaaagaaaa aactgcttca atggaaaatg ttaaacttgt
     1201 tcatggtgat gcaatggaat tgccgtttga agacaattct tttgattatg taacaattgg
     1261 gtttggatta agaaatgtgc cagactattt agtcgcgtta aaagaaatga atagagtact
     1321 taaaccaggt ggtatggtgg tatgtcttga aacgagccaa ccaactttgc cagtctttaa
     1381 acaaatgtat gcactatact ttaaatttgt tatgcctatt tttggaaaat tatttgcaaa
     1441 atcaaaagaa gaatatgaat ggttacagca atctacgttt aattttcctg gaaaagaaga
     1501 gttgaagcgc atgtttgaag aagcaggttt cataaatgta agagtacgta gttttacagg
     1561 gggcgttgct gcaatgcacc ttggctataa agaaaaagat aataccaaag gtgattaacg
     1621 tggcaaagtt aaacatgaac aatgaaatta agaaagtgga acaacgactt gaaaaggcaa
     1681 taaaaagtaa agattctgta ttagaacagg catcattaca cttattgtct tctggtggta
     1741 aaagagtacg cccagcattt gttattctga gtagccaatt tggcaaagat gaacaaacgt
     1801 ctgaacaaac gtatcaagtt gcagtcgcat tagagttaat tcatatggca acacttgttc
     1861 atgatgacgt tattgataaa agcgacaagc gtcgaggcaa gttaaccata tcaaagaaat
     1921 gggatcagac aactgctatt ttaactggga attttttatt ggcattagga cttgaacact
     1981 taatggccgt taaagataat cgtgtacatc aattgatatc tgaatctatc gttgatgttt
     2041 gtagagggga acttttccaa tttcaagacc aatttaacag tcaacagaca attattaatt
     2101 atttacgacg tatcaatcgc aaaacagcac tgttaattca aatatcaact gaagttggtg
     2161 caattacttc tcaatctgat aaagagactg tacgaaaatt gaaaatgatt ggtcattata
     2221 taggtatgag cttccaaatc attgatgatg tattagactt cacaagtacc gaaaagaaat
     2281 taggtaagcc ggtcggaagt gatttgctta atggtcatat tacgttaccg attttattag
     2341 aaatgcgtaa aaatccagac ttcaaattga aaatcgaaca gttacgtcgt gatagtgaac
     2401 gcaaagaatt tgaagaatgt atccaaatca ttagaaaatc tgacagcatc gatgaggcta
     2461 aggcagtaag ttcgaagtat ttaagtaaag ctttgaattt gatttctgag ttaccagatg
     2521 gacatccgaa atcactactt ttaagtttga cgaaaaaaat ggggtcaaga aacacatagt
     2581 atttatgaaa agtattgaaa gcgctttacc aacctgttaa tatataatag taatatacat
     2641 aaacctatta aacacagggg gatacataaa gtggaacgta catttttaat gattaaacca
     2701 gacgcagtac aaagaaatct aattggtgaa gtaatttcaa gaattgaaag aaaaggacta
     2761 aaacttgtcg gtggtaaatt aatgcaagta ccaatggaac ttgctgaaac acattatggt
     2821 gaacaccaag gcaaaccgtt ttataatgat ttaatttcat ttattacatc agcaccagtg
     2881 ttcgcaatgg tagttgaagg tgaagatgca gttaatgtat ctagacatat tattggcagc
     2941 accaatcctt cagaagcttc accaggatca attagaggtg atttaggttt aactgttggt
     3001 agaaatatca ttcacggttc agattcatta gagtctgctg aacgtgaaat taatctatgg
     3061 tttaatgaaa atgaaattac tagctatgct tcaccacgtg atgcatggtt atatgaataa
     3121 aatataaact gtaaaccttt acgatttatt tataaaggta gaaagggttt tgttatgtgg
     3181 ttagtcatta tgattataca taacaaggcc cgttttttat gttgtagtaa attacttgaa
     3241 aaattttata gttttttggt aacacgtatt aaaaagagag gaatattctt tatcaaatga
     3301 aactaaacag agagaagggg ttgttaaaat gaagaatatt atttcgatta ttttggggat
     3361 tttaatgttc ttaaaattaa tggaattact atatggtgct atatttttag ataaaccact
     3421 taatcctata acaaaaatta tttttatact gactctcatt tatatttttt atgtattagt
     3481 aaaagaattg attatatttt tgaagtcaaa gtataacaaa agcgcttaac atatgtatat
     3541 tttaatatca taattttttt aaacggactg attaacttta ttaataatta acagttcgtt
     3601 cttttgtatt aagaaatgta gtcagtatat tatttgctaa agttgcgata cgattatatt
     3661 aaaacggcta atcattttta attaatgatt atatgatgca actgtttaga aattcatgat
     3721 acttttctac agacgaatat attataatta attttagttc gtttaatatt aagataattc
     3781 tgacatttaa aatgagatgt catccatttt cttaattaag cttgaaaaca aacatttatg
     3841 aatccacaat gaatgtgata agattaacaa catattataa tgttattgcg gaagtatgaa
     3901 aggagcgagt gtgtatgaga tatctaactt caggagaatc acatggacct caattaacag
     3961 ttattgtcga aggtatacca gcaaatttag aaattaaagt tgaagatatt aataaagaaa
     4021 tgttcaagcg tcaagggggt tatggtcgtg gccgacgcat gcaaattgag aaagatacag
     4081 tagaaatagt atcaggcgtt agaaatggtt atacattagg tagtccaatt actatggttg
     4141 taaccaatga tgactttacg cattggagaa aaattatggg agcagctcca ataagtgaag
     4201 aagaacgtga aaatatgaaa cgtactatta caaaaccaag acctggtcat gcagatttgg
     4261 ttggaggtat gaaatataat catcgtgatt tacgaaatgt gctagagcga tcatctgcta
     4321 gagaaacagc agctcgagtt gcagtcggtg ccttatgtaa agtgttatta caacagttag
     4381 atatcgatat atacagtcgt gttgttgaaa taggtggcat taaagataaa gatttttatg
     4441 attcagaaac atttaaagca aatcttgatc gtaatgatgt tcgtgtaatt gatgacagta
     4501 tcgcacaagc aatgcgagat aaaattgacg aagctaaaaa tgaaggagat tcaattggcg
     4561 gtgtcgttca agttgtagtt gaaaatatgc ctgttggtgt tggtagttat gtgcattatg
     4621 atcgtaagtt agatggtaag attgcacaag gtgttgtcag cataaatgct tttaaaggtg
     4681 taagctttgg agaaggattt aaagcagctg aaaagccagg tagtgagatt caagatgaaa
     4741 ttctatataa tagtgaaatt ggttattatc gtggatctaa tcatttaggt ggtttagagg
     4801 gtggtatgtc aaatggtatg ccaattattg taaatggtgt aatgaaacct attcctacgt
     4861 tatataaacc attaaattca gttgatataa atactaaaga agactttaag gcaacaattg
     4921 aacgttctga tagttgtgct gtacctgcag caagtatagt gtgtgaacac gttgttgcat
     4981 ttgaaatagc gaaagcatta cttgaagagt ttcaatcaaa ccatattgaa caacttcaac
     5041 aacaaattgc tgaccgcaga caattaaatg ttgagtttta acaacgagaa caattgaggt
     5101 gtgatcatga aattacaaac aacctatcct tcaaacaatt atccaatatt tgttgaacat
     5161 ggtgcaattg accatattag cacgtatatt gatcagtttg atcaaagttt tatattaatt
     5221 gacgagcatg taaatcaata ttttgctgat aaatttgatg atattttatc atatgaaaat
     5281 gtgcataaag tcattattcc agctggtgaa aagacgaaaa catttgagca atatcaagaa
     5341 acattagaat acattttgtc acatcatgta acgcgtaata cagcgattat agctgttggt
     5401 ggtggtgcga caggagattt tgcaggattt gtagcagcaa cactattaag aggtgtccat
     5461 tttatacaag ttcctacaac gattttggcg catgattcta gtgttggcgg taaagtgggt
     5521 attaactcaa aacaaggtaa aaaccttatc ggtgcatttt atcgtccaac tgctgtgatt
     5581 tatgatttag actttttaaa gacgttacca tttgagcaaa tattaagtgg ctatgcagaa
     5641 gtttataagc atgcgttatt gaatggtgaa tcaacgacgc aagaaatcga acagcacttt
     5701 aaagatagag agatattaca gtcattaaat ggtatggata aatatattgc taaaggtatt
     5761 gaaacgaagc tggatattgt tgttgcagat gaaaaagaac aaggtgtacg taaattttta
     5821 aatttaggtc atacatttgg tcatgccgtg gagtataacc acaaaattgc acacggtcat
     5881 gccgtaatga taggcataat atatcaattt attgttgcga atatattgtt caattctaat
     5941 cacgatatcc aacattatat taattattta acgaaattag gttatccttt agaaacaata
     6001 accgacatag atttcgaaac gatatatcaa tacatgctaa gtgataaaaa gaacgacaaa
     6061 caaggagttc aaatggtatt gattaaacat tttggcgata ttgtagtgca acacattgat
     6121 caaacaacac tgcaacatgc gtgtgaacaa ctaaaaacat attttaagta ggtgaagtaa
     6181 atggtaagtg aacaaattat cgatatttca gggccactta aaggtgaaat agaagtgcct
     6241 ggagataagt cgatgacaca tcgagcaatc atgttagcat ctttagctga aggaacttcc
     6301 aatatttata aaccattact tggtgaagat tgtcgtcgta caatggatat attccgatta
     6361 ttaggcgtag atattaaaga agatgaagat aaattggttg tgaattcacc tggatataaa
     6421 gcttttaaaa cacctcatca agttttatat actggaaact ctggcacaac gactcgattg
     6481 ttagctggtt tgttaagtgg tttaggtatt gaaagtgttt tgtctggcga tgtttcaatt
     6541 ggtaaaaggc caatggatcg tgtcttgaga ccattgaaac ttatggatgc gaatattgaa
     6601 ggtattgaag ataattatac accattaatt attaagccat ctgtcataaa aggtataaat
     6661 tatcaaatgg aagttgcaag tgcacaagta aaaagtgcca ttttatttgc aagtttgttt
     6721 tcaaatgata caacggtaat aaaggaatta gatgtaagtc gaaatcatac tgagacgatg
     6781 ttcagacatt ttaatattcc aattgaagca gaaaggttat caataactac aacccctgat
     6841 gccattcaac atattaaacc tgcagatttt catgttcctg gcgatatttc atctgcagcg
     6901 ttctttattg ttgcagcact tatcacacca gaaagtgatg taacaattca taatgttgga
     6961 atcaatccaa cacgttcagg tattattgat attgttgaaa aaatgggcgg taatatccaa
     7021 cttttcaatc aaacaactgg tgctgaacct actgcttcta ttcgtattca atacacacca
     7081 atgcttcaac caataacaat cgaaggagaa ttagttccaa aagcaattga tgaactgcct
     7141 gtaatagcat tactttgtac acaagcagtt ggcacgagta caattaaaga tgccgaggaa
     7201 ttaaaagtaa aagaaacaaa tagaattgat acaacggctg atatgttaaa cttgttaggg
     7261 tttgaattac aaccaactaa tgatggattg attattcatc cgtcagaatt taaaacaaat
     7321 gcaacagttg atagtttaac tgatcatcga ataggaatga tgcttgcagt tgcttctcta
     7381 ctttcaagcg agcctgtcaa aatcaaacaa tttgatgctg taaatgtatc atttccagga
     7441 tttttaccaa aactaaagct tttagaaaat gagggataat ataaaatgga agatatctat
     7501 aaattaatag acgatatcaa tctacaaaaa ctagaaaatt tagactctcg tgttaatgaa
     7561 gcaataacta ctgacaacga tgacgcatta tttattctag gagagacact ttacaatttt
     7621 ggattaatgc cacaaggttt ggaagtattc cgcgtgttat atcacaaata tccagacgaa
     7681 agtgaattgc tgatttattt tattgaaggt ttaatgtctg aaaatcaaac tgacgaagcg
     7741 ttagaatatt taagttatgt tgaaccatca cctgaaaagt tgatgttaga agcagattta
     7801 tatcaacaaa ttaatatgat ggaagttgct attgataaat tacaagaagc acttgaacta
     7861 gagccaaatg atccaataat ccattttgca ttggctgaaa tgttatatta tgatggtcaa
     7921 tatttacgtg ctacctctga atacgaaacc gttttagaaa ctggtgaata tcaagttaat
     7981 ggtgtaaact tattctctcg tatggcagat tgtagtttac aaagtggtaa ctatagtgat
     8041 gcgattcgct tatacgatga aattaatgaa gatgaaatga cttcagaaga ttatctcaaa
     8101 aaagccattt cttacgataa aaatgacatc actcaagaag caattaaaat aatgactaca
     8161 ttactttcta aagatcctga ttatattcaa ggctacttgt atttacaatc attatatgaa
     8221 aatgaaaaaa attatccaga tgccattgag acgggtaaag aaggattacg actaagtcaa
     8281 ttttataaag agttaatgta tacaactgga tgtttggaaa tagaacacgg cgatgctaat
     8341 gaaggtgtat tattgttaaa acaagcatta gaggttgata atgcttacca agaaccttta
     8401 ttgattttaa gcgatttata tcgtaatgaa gaagattatg aagcaatcat tgaattatta
     8461 acatatgtag atgaagaaga tttggatcct acattcatgt ggcatttagc gttcgcctat
     8521 ggtcaagaag agcgagataa agaagctcaa catttctttg aattagcata tccaacgatg
     8581 aaaacaaata ttgattttat gagtgattat tatttctatt taattgaaat tggtcaaaag
     8641 gaacaagcaa ttactatttt aaatcaattg ttggaactag agccaagtaa cgaaaattgg
     8701 catgacgaat tatcaagatt gcaatcttaa aatggggtat ttgatgtcag agacactcaa
     8761 tcaaattaaa gaaagtttta ttgaatattt actttttcaa tatcgattta agtcgcgtat
     8821 tgcagtttgg gtgcttaact atataaaagt gaatgaggcg aaattggcaa acattcattt
     8881 tgtagataca aaaattaatc atcatgaaac attagaaatt gcagaagtgg gaagtcacgc
     8941 ttctgcaatt caatttacta agcgtaatat aaagttaatg aatacaaatg aaatatttga
     9001 ttatatcgct aatcataact gtgcttttga tattcaaatt cacttcgcaa acgtgtcaaa
     9061 gcgtgagcaa aggctagatg atttaatagt tgctcaacta acggaatcgc ccagttatca
     9121 aacttatctt catgacttaa atagtatggc tatagatcgg cataaacatg cgttattaat
     9181 agattacttg ttacacaata ttgatttaag tttacagatg aatgaaaagc aacgttttta
     9241 tcaactaacg caaattttaa atacactcaa gctagtaaat aaacataatc aatttgagga
     9301 cttagcagat gacgattaat acattttggc aatatacact ttatcaacgt tcttggttaa
     9361 tgatgctact catttgtaat atattaggga tgatttatgg ttatatatgg tatggtgaac
     9421 aattaagtca tacgccgtgg cattttaaga tttttgttcc agatagccca actgcaatat
     9481 tatacttagt tatttctatc tcattgatat taattcaaaa acaaaatagt attatcgaag
     9541 cactggcatt tgtaacatta ttcaaatatg ggatttgggc agttataatg aatatattat
     9601 ttattattga acaaggtgat attacagtaa atggtttggt attaatgttt tcccacagta
     9661 taatggctgt tcaggcaatt tacttctatc ctaggtttaa acgttctatg attggaatca
     9721 gtgtagcgat gatttgggtg tttttgaatg attatattga ctattttcac cttcaatttc
     9781 catattatga ttttataact acacatgtgt ggcaaattgg tgtattatca tgttgtctaa
     9841 gtgtttttgg ac
//
LOCUS       DAOBBH010000094         9829 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.094, whole genome shotgun sequence.
ACCESSION   DAOBBH010000094 DAOBBH010000000
VERSION     DAOBBH010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9829
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.094"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            90..1124
                     /locus_tag="P6L01_001637"
     CDS             90..1124
                     /locus_tag="P6L01_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDP3030706.1"
                     /translation="MKDVTIIGGGPSGLYASFYAGLRDMSVRLIDVQSELGGKMRIYP
                     EKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKAERHFEVETEAGE
                     IYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTA
                     LDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHI
                     SEVVLEHVESGDRHTVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTS
                     IPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEA
                     NKTIVNKHLY"
     gene            1160..1561
                     /locus_tag="P6L01_001638"
     CDS             1160..1561
                     /locus_tag="P6L01_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDP3030707.1"
                     /translation="MVKVTYDIPTCEDYCALRINAGMSPKTREAAEKGLPNALFTVTL
                     YDKDRLIGMGRVIGDGGTVFQIVDIAVSKSYQGQDYGSLIMEHIMKYIKNVSVESAYV
                     SLIADYPADKLYAKFGFMPTEPDSGGMYIKY"
     gene            complement(1607..2611)
                     /locus_tag="P6L01_001639"
     CDS             complement(1607..2611)
                     /locus_tag="P6L01_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDP3030708.1"
                     /translation="MIETFKAFVIDKDESGKVTPTFKQLSPTDLPKGDVLIKVHYSGI
                     NYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGG
                     FSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGA
                     SGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASST
                     WQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVF
                     TAMKLRQRVWRRLAKDLKPENLHEIKQVITFDELPEQLNKVINHENKGRIVIDFGVDK
                     "
     gene            2802..3317
                     /locus_tag="P6L01_001640"
     CDS             2802..3317
                     /locus_tag="P6L01_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDP3030709.1"
                     /translation="MAGIIKEISEQNASELVELATRTFYDTFGSYYDDKDFDQFFKDN
                     YTVEKFTQEINHVDSFHYFYQEDGANVGYIKMNINSAQTEEMGETYLEVQRIYFLKDF
                     QGGGRGSQLIELAEKIAQEHNKHKIWLGVWEHNPRAQAFYKRHGFKVVGEHHFQTGDV
                     TDTDLIMEKEL"
     gene            3631..4245
                     /locus_tag="P6L01_001641"
     CDS             3631..4245
                     /locus_tag="P6L01_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030710.1"
                     /translation="MKRLVTGLLALSLFLAACGQDSDQQKDSNKEKDDKAKTEQQDKK
                     TNDSSKDKKDNKDDSKDVNKDNKDNSANDNQQQSNSNATNNDQNQTNNNQSSNNQKSS
                     YVAPYYGQNAAPVARQIYPFNGNKTQALQQLPNFQTALNAANNEANKFGSNNKVYNDY
                     SIEEHNGNYKYVFSFKDPNANGKYSIVTVDYTGQAMVTDPNYQQ"
     gene            complement(4399..6072)
                     /locus_tag="P6L01_001642"
     CDS             complement(4399..6072)
                     /locus_tag="P6L01_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDP3030711.1"
                     /translation="MIKQINVSNMQKFESQLMKAQSEGYTHVVPYANEIMIYQSMLDA
                     VQLYPKSIVVDYTVDGQYKNDCHYFGQSSINIADWAQNNNYYPNLIYAIQQTLDLIHY
                     YSVETIFDLALLTLLKGDLSIDGHVVFDFKAPIATSASIWETIKTIEDFDMMSQFYLN
                     KMAYIDHHPIPFRNLFIEDSEQLNSPDNWLYSTKFMLPKWLYKIAKQRADNKQLQNFG
                     LYTKQPNVLKDHIVFIGDHHQYIGNSKYLFTYFVKHNPMTACYFVTDDRRGPHFISPK
                     SEKADELINSARVVIVENDIPETLQPNGTLIQLHQGTPIMQLFLDSKEPIKNIETPFY
                     RAKRYNRWLQFDYVIHSADDISHFYQTAFPSHQANVLAYGNPKHQYLLQKRNESTTQQ
                     QYKKSFKINDQKPVLLYAPIGLVSAQQLPLSDALFKAYHVVVQGVDEAILPEEALVAP
                     KYLSAQDLILMSDVVITDYSNIIFDAMAIDKTVALYTPNHSQYIESQGVNEDIWRHLS
                     KIWYTDRQLLINNLISQAIPVIKYPQIQHKEQPLESISQLILSKMTSNQ"
     gene            6389..7987
                     /locus_tag="P6L01_001643"
     CDS             6389..7987
                     /locus_tag="P6L01_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDP3030712.1"
                     /translation="MLVNTFNPFDNLLLSSLIAAIPIVLFLLCLTVFKMKGIYAAITT
                     LVVTLLIAIPFFKLPVGIASGAVVEGFFQGIIPIGYIVMMAVLLYKITVESGQFLTIQ
                     DSITNISQDQRIQVLLIGFAFNAFLEGAAGFGVPIAICALLLTQLGFNPLKAAMLCLV
                     ANAASGAFGAIGIPVGVVETLKLPGDVSVLGVSQSATLTLAIINFIIPFLLIFIIDGF
                     RGVKETLPAILVVSITYTLTQGLLTVFSGPELADIIPPLLTMLALAVFSKKFQPKHIY
                     RVNKDEEIEPAKAHSAKAVLHAWSPFIVLTVIVMIWSAPFFKNLFLPNGALSSLVFKF
                     NLPGTISEVTHKPLVLTLNIIGQTGTAILLTIIITILMSKKVNFKDAVRLFGVTFKEL
                     WLPVLTICFILAISKITTYGGLSAAMGQGIAKAGNVFPVLSPILGWIGVFMTGSVVNN
                     NSLFAPIQASVAQQIGTSGSLLVSANTVGGVAAKLISPQSIAIATAAVKQVGKESELL
                     KMTLKYSVCLLIFICIWTFILSLL"
     gene            8367..>9829
                     /gene="mqo"
                     /locus_tag="P6L01_001644"
     CDS             8367..>9829
                     /gene="mqo"
                     /locus_tag="P6L01_001644"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDP3030713.1"
                     /translation="MTTQHSKTDVILIGGGIMSATLGTLLKELSPEKNIKVFEKLAQP
                     GEESSNVWNNAGTGHSALCELNYTKEGKDGTVDCSKAIKINEQYQISKQFWAYLVKTG
                     QLDNPDRFIQAVPHMSFVIGEDNVAFIKSRVATLKKSVLFEKMKLSQDEEEMKSWVPL
                     MIEGRKSDEPIALTYDETGTDVNFGALTAKLFENLEQRGVGIQYKQNVLDIKKQKSGA
                     WLVKVKDLETNETTTYESDFVFIGAGGASLPLLQKTGIKQSKHIGGFPVSGLFLRCTN
                     QEVIDRHHAKVYGKAAVGAPPMSVPHLDTRFVDGKRSLLFGPFAGFSPKFLKTGSHMD
                     LIKSVKPNNIVTMLSAGIKEMSLTKYLVSQLMLSNDERMDDLRVFFPNAKNEDWEVIT
                     AGQRVQVIKDTEDSKGNLQFGTEVITSDDGTLAALLGASPGASTAVDIMFDVLQRCYR
                     DEFKGWEPKIKEMVPSFGYRLTDHEDLYHKINEEVTK"
BASE COUNT         3393 a         1426 c         1805 g         3205 t
ORIGIN      
        1 taatagtata atggctttaa atgataataa ttatcaattt gagcacgtta atgaaagaaa
       61 tgaatataga gagtcatagg aggcacataa tgaaagatgt tacaatcatt ggtggcggtc
      121 cgtctggttt gtatgcaagc ttttatgctg gattgcgaga tatgtctgta agattgatag
      181 atgttcaatc tgaattaggg ggtaagatga gaatttaccc tgaaaaaatt atttgggata
      241 ttggcggcat tgcgccaaaa ccttgtcatg aaattttaaa agatacaatt aagcaaggat
      301 tatattttaa accggaagtt catttgaatg aacgtgtggt agatattagg aaaaaggcag
      361 aacgtcattt cgaagttgaa acagaagcgg gagaaatata tacatcgaaa gcagttatca
      421 ttgcaattgg tgcgggtatt attaatccaa aacaattaga tgttaaaggt gtagagagat
      481 atcaattaac taatttacat tatgttgtac aaagttacag acgtttcaaa gataaagatg
      541 tattaatatc aggtggagga aatacagcat tagattgggc gcatgatatt gccaagattg
      601 ctaaaagcgt gacagttgtt tatcgaaaag aagatgtaag tggtcatgaa gcgatgaaaa
      661 cgttggtgac agatttaaat gtgaaactat gcccaaaaac acgtattaaa tacttggtcg
      721 gcaatgatga tgaaacgcat ataagtgaag ttgttttgga acatgttgaa agtggcgata
      781 ggcatacagt taaatttgac gatgtcatta ttagtcatgg gtttgatcgt tgtaatacat
      841 tattgagtga aacatcttca aagctagata tgcatgatga ttgtcgtgtt aaaggatttg
      901 gtaatacgac aactagtata cctggtatct atgcgtgtgg agatattgtt tatcatgatg
      961 caaaatcaca tctaattgca agtgcattta gtgatggtgc aaatgcagcg aaccttgcta
     1021 aaacatatat ccaaccagat gcgaatgcag agggttatgt ttcaagtcat catgaagtgt
     1081 ttaaagaagc gaataagact attgtaaata aacatttata ctaggttgat atattgtgtg
     1141 atgaaaagtg gggtaaataa tggttaaagt gacatatgat attccgactt gcgaggatta
     1201 ttgcgcatta aggattaacg caggtatgag tccaaagacg cgcgaagcag ctgaaaaagg
     1261 attacctaat gccttattta cagtaacctt gtatgataaa gatcggttaa ttggtatggg
     1321 tagagtgatt ggcgatggcg gaactgtttt tcaaattgtt gatattgctg tttcgaaaag
     1381 ttatcaaggt caagattacg gcagtctaat tatggaacat attatgaagt atattaaaaa
     1441 tgtatctgtc gaaagtgcat acgttagtct gatcgcagac tacccagcgg ataaattata
     1501 tgcaaaattt ggatttatgc ctaccgaacc agattcaggc ggtatgtata tcaaatacta
     1561 aattaaaaaa gacatctcgc ataacgggat gtctttttca tgaatactat ttatctacac
     1621 cgaaatcgat aacaatgcgg cctttatttt catgattaat tactttgtta agttgttctg
     1681 gaagttcatc aaatgtaata acttgcttga tctcatgtaa attttcaggc tttaaatctt
     1741 ttgcgagacg acgccaaacg cgctgtctta atttcatagc agtaaatact gagtcaattc
     1801 ctaaaatgtt tacgcctctt aaaatgtgag ggaatacaga attagtataa gtattgccgg
     1861 cagtcatacc aataactgca attgatccac tatgatttaa acgttttgta acataattaa
     1921 taccttcgcc accaacaggg tctacacaag cttgccaagt tgatgatgcg agtggctttt
     1981 tatgatcatc ttcaacagga agtcgatcga taacttcttt ggcaccaagt tcaagtaatt
     2041 gatcgctaac atcttgttta cctgtacttg cgataacttt ataaccaagt tcattaagca
     2101 tgagtactgc taaagtaccg acaccacctg aagcaccgcg aacgagtaca ggaccatctt
     2161 caatattcat tccaactttt tcaagtcttt caattgctaa accggcagta taaccagctg
     2221 tgccatatat cattgattct tctaatgtta aagtatcagg aagcttgata atccattctg
     2281 attttacacg cgcatattca ctaaaaccgc cataatggct aacacctagg tcataactcg
     2341 ttacaattac ttgttctcct ttttcaaagc ctggtgcttc ggattcaaca attgttccag
     2401 ctaaatctat tcctggaatc ataggatacg attttacgac tgcattatga tcttgagtcg
     2461 ctaaagcatc tttataattt ataccagagt aatgtacttt aatcagcaca tctcctttag
     2521 gtaaatcagt aggcgataat tgtttgaaag ttggtgtcac tttaccgctc tcatctttat
     2581 caattacaaa cgctttaaat gtttctatca caacgataca tcctctcttt ttctcttttg
     2641 aaaatacggc cattttactg gaataaatgt atcatttatt gatgttacac acaatcaata
     2701 caatttgtaa ttgatttgtg ttaatgtatt tttaaatagt tttggctgaa aaataacata
     2761 agtaatattt tgattcgatc actgtttatt ggaggattta tatggctggg ataatcaaag
     2821 aaatttcaga acaaaatgct agtgaattag ttgaattagc aactagaaca ttttatgaca
     2881 cgtttggttc ttactatgat gacaaagatt ttgatcaatt ttttaaagac aattatactg
     2941 tagaaaaatt tacacaagag attaaccatg tagattcatt tcattatttt tatcaagaag
     3001 atggtgcgaa tgttggttat ataaagatga atattaatag tgctcaaact gaagaaatgg
     3061 gggagaccta tttagaagtg cagcgcatat attttttgaa agactttcaa ggtggcggaa
     3121 gaggttcaca attgatagaa ttggccgaaa aaattgctca agaacataat aaacataaaa
     3181 tttggctagg agtttgggag cataatcctc gtgctcaagc attttataaa cgtcatggat
     3241 ttaaagtggt tggtgaacat cattttcaaa caggtgatgt aacggataca gatttaatta
     3301 tggaaaaaga gttataatga ttgtttttgc agcagataaa atgaaatcat agtaataaat
     3361 attgtgtatg ccttgaaatg ctaaatgcgt aatgaattgt taaagtaaaa taattaaatt
     3421 tatagaaaac ataaaattca caaaaattaa ataataatga acaaaaatta gggtataata
     3481 aaaagaacct tagattgtag ttatgggtat tgtgataatt caaatcatat taatgttgac
     3541 gtctttggta acaagccatg aaatgataca catggtagat atgtatttca agcttattca
     3601 atgaatatcg aattatagga ggagatatgt atgaaaagat tagttacagg gttactagca
     3661 ttatcattat ttttagctgc atgtggtcaa gatagtgacc aacaaaaaga cagtaataaa
     3721 gaaaaagatg ataaagcgaa aactgaacaa caagataaaa aaacaaatga ttcatctaaa
     3781 gataagaaag acaataaaga tgatagtaaa gacgtaaaca aagataataa agataatagt
     3841 gcaaacgata accagcaaca atctaattca aatgcaacaa acaatgacca aaaccaaaca
     3901 aataataacc aatcaagtaa taatcaaaaa tcaagttacg ttgcaccata ttatggacaa
     3961 aatgccgcgc cggttgcacg tcaaatttat ccgtttaatg gaaataaaac tcaagcttta
     4021 cagcaattgc caaatttcca aacagcttta aatgcggcta ataatgaagc aaataaattt
     4081 ggtagtaata ataaagtgta taatgattat tctattgaag aacataatgg caactataag
     4141 tatgtgttta gttttaaaga cccaaatgca aatggaaaat attcaattgt aacggttgat
     4201 tatactggac aagcaatggt tactgatcca aactaccaac aataatgtta atataactat
     4261 gatgcaagtt aaaaaaataa aacggtaaac tctataatat gaattagggt ttaccgtttt
     4321 ttgcgtattt taaagtataa aagtttacgt tttatagcac ttttaaacct cattcacagc
     4381 cactgagaaa tttaaacact attgattaga tgtcatctta gacaatataa gttgtgaaat
     4441 agattctaat ggttgttctt tatgttgtat ttgtggatat ttaatcactg gtattgcctg
     4501 ggaaattaaa ttattaatta ataattgtcg gtctgtatac caaatttttg atagatgacg
     4561 ccaaatatct tcgttaacac cttgagactc aatgtattga ctgtgatttg gagtgtataa
     4621 tgctactgtt ttgtctatag ccatcgcatc aaatattata tttgagtaat cagtgataac
     4681 gacatcagac attagaatta agtcttgagc actaagatac tttggtgcaa caagcgcctc
     4741 ttctggtaag atcgcttcat caacaccctg aaccacaaca tgataggctt tgaataaagc
     4801 atctgatagt ggtaattgtt gcgcactcac taatccaata ggtgcgtata ataaaactgg
     4861 tttttgatca tttattttaa acgatttctt atactgttgt tgagtagtac tttcgttacg
     4921 cttttgtagt aaatattggt gtttaggatt accataagcc aatacatttg cttggtgact
     4981 aggaaaagcc gtttgataaa agtggctaat atcatcagca gaatgtataa cataatcgaa
     5041 ttgcaaccag cgattatacc ttttagcacg ataaaaaggt gtttctatat ttttaatagg
     5101 ctctttacta tctaaaaata attgcattat tggtgtgcct tgatgtaatt gaattaaagt
     5161 accgtttggt tgtaacgttt caggtatatc attttcaact ataactacgc gtgcgctgtt
     5221 aatcaattcg tcagcttttt cagacttagg tgatatgaaa tgtggtccgc gacgatcgtc
     5281 agtaacaaaa taacaggcag tcattggatt atgtttaacg aagtaagtaa ataaatattt
     5341 tgaattacct atatattgat gatgatcacc gataaaaacg atgtgatctt ttaaaacgtt
     5401 tggttgcttc gtatacaggc cgaagttttg taattgtttg ttatcagcac gttgctttgc
     5461 tattttatat aaccatttag gtagcataaa cttggtagaa tacaaccaat tatctggcga
     5521 attaagttgt tctgaatcct cgataaaaag gttgcgaaaa ggtataggat gatgatcaat
     5581 ataagccatt ttattcaaat aaaactgaga catcatatca aaatcttcaa tagttttaat
     5641 agtttcccat attgaagcac ttgttgctat aggggcttta aaatcaaaaa cgacatgacc
     5701 atcgattgat aaatcacctt tcagaagtgt caataaagct aaatcgaaaa tcgtttctac
     5761 agagtaataa tgtattaaat caagagtctg ttgaatcgca tatatcaggt taggataata
     5821 attattgttt tgagcccaat cggcaatgtt gatcgatgat tgaccgaaat agtgacaatc
     5881 atttttgtat tgtccatcca cagtataatc aaccacgata gacttaggat ataattgaac
     5941 cgcatctaac atcgattgat aaatcattat ctcatttgca tatggaacga catgtgtata
     6001 accttcagac tgagctttca ttaattgaga ttcaaacttt tgcatattag aaacatttat
     6061 ctgtttaatc attgctataa cttccatttc tcatcgtagt atcttcctta ttttagcacg
     6121 gtcttatgta aaattcatga atagtataag ttacttcatt acattaagta caattcattt
     6181 tgatgaacag atttaaaatg aaaaataaaa caattaaata ggaaagtgat attgttaata
     6241 gtctgaaaaa atggtatatt ttaattgtga aaaaaatcac attaatctca ctgttctgta
     6301 aaatgttatc gataacaaaa tgctatagcc agagcatgat tttaatgaag caattacact
     6361 actactttat ggaaagaaga gtcagcctat gttagtaaat acgtttaacc catttgataa
     6421 tttattatta tcgtctttaa ttgcagcaat accgattgta ctgtttttac tatgtttaac
     6481 tgtttttaaa atgaaaggta tttatgcagc tattacaaca cttgttgtaa cattactaat
     6541 tgcaatacca tttttcaaat tgccagttgg tatcgcttct ggtgcagtag tcgaaggttt
     6601 cttccaaggt atcattccga ttggctatat cgttatgatg gcagtattgt tatacaaaat
     6661 tactgttgaa tctggacaat ttttaacaat tcaagatagt attacaaata tttcacaaga
     6721 ccaacgtatt caagttttac ttattggatt tgcattcaac gcatttttag aaggtgcagc
     6781 aggatttggt gtaccaattg caatttgtgc acttttatta acacaattag gatttaatcc
     6841 attaaaagct gcgatgttat gtttagtcgc aaatgcagcg tctggtgctt ttggtgcgat
     6901 tggtatccct gtaggtgttg tagaaacgtt gaaattacct ggagatgttt cagtattagg
     6961 tgtttctcaa tcagcaactt taacattggc aatcataaat ttcattattc cattcttact
     7021 tatctttatt attgatggtt ttagaggtgt taaagaaaca ttaccagcaa ttttagtagt
     7081 ttcaatcact tatacactta ctcaaggatt attaactgta ttcagtggac ctgaattagc
     7141 agatattatt ccaccgttat taacaatgtt agcattagca gtattttcta aaaaattcca
     7201 accaaaacac atttatcgtg ttaataaaga tgaagaaatt gaacctgcaa aagcacattc
     7261 tgcaaaagca gtattacatg catggagccc attcattgta ttaacagtca ttgtaatgat
     7321 ttggagtgcg ccattcttta aaaacttatt cttaccaaat ggtgctttat catcattagt
     7381 atttaaattc aacttacctg gaacaatcag cgaagttacg cataaaccat tagtattgac
     7441 tttaaatatt attggacaaa caggtacagc tattttatta actattatta ttacaatttt
     7501 aatgtctaaa aaggttaact ttaaagatgc tgttagatta ttcggcgtta catttaaaga
     7561 gttgtggtta ccagttctta caatttgttt catcttagca atttctaaaa tcacaactta
     7621 tggtggttta agtgcagcaa tgggtcaagg tatagctaaa gcaggtaatg tcttcccagt
     7681 tctatcacca atcttaggtt ggataggtgt gtttatgaca ggatcagttg taaataacaa
     7741 ttcattattt gcaccaattc aagcttctgt tgcacaacaa attggaacaa gtggttcact
     7801 tcttgtatct gctaatacag ttggtggtgt agcggcaaaa ttgatttcac cacaatcaat
     7861 tgcaattgca actgcagcag taaaacaagt tggtaaggaa tcagaattat taaaaatgac
     7921 attgaaatac agtgtatgtt tactaatatt catctgtatt tggactttca tcttgtcatt
     7981 attataaaaa aacgtatttc aaaatataaa tatacagaag gtgagatgtt ttctaacatc
     8041 tcatcttttt ttatggatca ttaatgaaag aagtttgaca ttataataat ggtagcgctt
     8101 tatgttaaaa tgaatagtga gtaatcagca atcaaattaa attggttgat agctgttaag
     8161 gtttgtggtt ttgtctttgt gctatcgcac ataaagtata taattaaagt agtttcgtta
     8221 ttataaaata ttaatataca tagtagatag taatagagca tcaccatggg aacctattga
     8281 gacacttatt gatttaaagt ggtattaata tgtcgtattt ctcgaacgtt ccattattca
     8341 ttttaaaaag ggggactgta tttgttatga caacacaaca tagcaaaaca gatgtcatct
     8401 taattggtgg cggtattatg agtgcaacat taggaacatt acttaaagaa ttatcacctg
     8461 agaaaaatat taaagtgttt gaaaaattag cacaacctgg cgaagagagt tcaaatgtat
     8521 ggaataatgc cggtacaggg cattcagcac tttgcgagtt gaactataca aaagaaggta
     8581 aggatggcac agttgattgt agtaaagcaa ttaagataaa tgagcagtac caaatttcaa
     8641 aacagttttg ggcatattta gttaaaacag gacaattaga taacccagat cgctttattc
     8701 aagcggtgcc acacatgagt tttgtcattg gcgaagataa tgtagctttt ataaaaagtc
     8761 gtgttgcaac gttaaagaaa agtgttttat tcgaaaaaat gaaattatcg caagatgaag
     8821 aagaaatgaa atcttgggta ccgttaatga ttgaaggtcg taagtctgat gaaccaattg
     8881 ctttaactta tgatgaaact ggtacagatg ttaactttgg tgcgttaact gcaaagttat
     8941 ttgaaaattt agagcaacgt ggtgtgggaa ttcaatataa gcagaatgta ttagacatca
     9001 agaaacagaa atctggggca tggctagtta aagttaaaga tttagaaact aatgaaacga
     9061 caacatatga atctgatttt gtatttattg gtgctggcgg tgcgagttta ccattactcc
     9121 aaaagactgg gattaaacaa tcaaaacata ttggtggttt cccagtaagt ggattattcc
     9181 tgcgctgtac aaatcaagaa gtgattgatc gtcatcatgc taaagtgtac ggaaaagcag
     9241 cagtgggtgc gccaccaatg tcagtgccgc acttagatac acgttttgta gacggcaagc
     9301 gttcattgtt atttggtcca tttgcaggtt tctcacctaa atttttaaaa acaggttcac
     9361 atatggattt aattaaatcg gttaaaccaa ataatatcgt gacgatgtta tctgcaggta
     9421 tcaaagaaat gagtcttacg aagtatttag tgtcacaatt gatgttatct aatgatgagc
     9481 gtatggatga tttaagagtc tttttcccaa atgctaaaaa tgaagattgg gaagtgatta
     9541 cagcagggca acgtgtccaa gtaatcaagg atactgagga ttctaaaggt aacttacaat
     9601 ttggtaccga agtcattacg tcagatgatg gcacattagc tgcattactt ggtgcatcac
     9661 ctggtgcgtc aacagctgta gatattatgt ttgatgtttt acagcgatgc tatcgtgatg
     9721 aattcaaagg gtgggaacca aagattaaag aaatggtgcc gtcatttggt tatcgcttaa
     9781 cagatcatga ggatttatat cacaaaatta atgaagaagt aactaagta
//
LOCUS       DAOBBH010000095         9693 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.095, whole genome shotgun sequence.
ACCESSION   DAOBBH010000095 DAOBBH010000000
VERSION     DAOBBH010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9693
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.095"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..298)
                     /locus_tag="P6L01_001645"
     CDS             complement(<1..298)
                     /locus_tag="P6L01_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDP3030714.1"
                     /translation="MKTKEVVALMNISQDTLRYYEKVGVIPPVNRDENGYRIYNDSDL
                     NWIYLVKNLRNAGVSIESLIEFCRLAQLPKNKNIQTQQKQILNKQLEELNENLKT"
     gene            542..709
                     /locus_tag="P6L01_001646"
     CDS             542..709
                     /locus_tag="P6L01_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030715.1"
                     /translation="MRKIWVIDRLYASTFFVDCLTVDSHLYTLTNFENLNHYLIILLI
                     AHAQHKKETCQ"
     gene            706..3135
                     /locus_tag="P6L01_001647"
     CDS             706..3135
                     /locus_tag="P6L01_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase 8 family protein"
                     /protein_id="HDP3030716.1"
                     /translation="MTYRMKKWQKLSTITLLMAGVITLNGGEFRSIDKHQIAVADTNV
                     QTPDYEKLRNTWLDVNYGYDKYDEKNDAMKKKFDATEKEAEKLLSSMKTESGRTYLWD
                     SAKDLDNKSADMTRTYRNIEKIAEAMRHPKTTLNTDENKKKVKDALEWLHKNAYGKEP
                     DNKVKELTENFKITDSSKKKALNWWDYEIGTPRSLTNTLILLNDQFSNEEKKKFTAPI
                     KTFAPKSDEILSSVGKAEPAKGGNLVDIAKVKLLESIIEEDKDMMKNSIDSFNKVFTY
                     VQDSATDKERNGFYKDGSYIDHKDVPYTGAYGVVLLEGISQMMPMIKETPFNDKTQNN
                     TTLKSWIDDGFLPLIYKGEMMDLSRGRAISRENETSHSASATVMKSLLRLSDAMDDST
                     KAKYKKIVKTSVKSDSSYGQNDTLSSYSDISKMKSLMEDSTISTNGLTQQLKIYNDMD
                     RVTYHNKDLDFAFGLSMTSKNVARYESINGENLKGWHTGAGMSYLYNSDVKHYRDNFW
                     ATADMKRLAGTTTLENEEPKGTDVKKSSKTFVGGTKFDDQHASIGMDFENQDKTLTAK
                     KSYFILNDKIVFLGTGIKSTDSSKNPVTTIENRKANGYTLYTDDKQTTASDNQGTNSV
                     FLESTNKPKNNIGYHFLNESKITVKKESHTGKWSDINKSQKQDSKTNQYYEVTQKHSN
                     TDSKYAYVLYPGLSKDDFNTKKDKVTVVKQDDDFHVVKDNESVWAGVNYSNSTQTFDI
                     NNTKVEVKAKGMFILKNKDDNTYECSFYNPESTNTASDIESKISITGYSITNKNTSTS
                     NESGVHFELTK"
     gene            complement(3195..4049)
                     /locus_tag="P6L01_001648"
     CDS             complement(3195..4049)
                     /locus_tag="P6L01_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDP3030717.1"
                     /translation="MNKTNFGDYLNKSKSRKIYKIFDQNLKREFSYWQFRKLFKSYTR
                     QSDVHELKVCTQNDDTMEQIWIDKANQFGVAVTWKDNSIIGFVMRPLINYDAIKNETT
                     LKYAVPTTDCYEVFWGGDNELYNYHYLYLHQRYAIDLVIKENHKTYKNQGKVNTDYFC
                     FGKDIIAPANGTVEKVVNGVQDNSIGSTNESQFLGNYIVIKHAENEYSLIAHLQQYSI
                     IVNEGQNVKYGDFLGKVGNSGNSTEPHIHFQVMNDKNIEACTSLKIRFLNNLELIKGD
                     VVCGLQGE"
     gene            complement(4260..5075)
                     /locus_tag="P6L01_001649"
     CDS             complement(4260..5075)
                     /locus_tag="P6L01_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HDP3030718.1"
                     /translation="MSKRLLLFDFDETYFKHNTNEEDLSHLREMEKLLEKLTNNNEVI
                     TAVLTGSTFQSVMDKMDQVNMTFKPLHIFSDLSSKMFTWNNGEYVESETYKKKVLSEP
                     FLFEDIEDILRHISAQYNVEFIPQRAFEGNETHYNFYFHSTGNHNNDSRILEALVRYA
                     NDQNYTARFSRSNPLAGDPENAYDIDFTPSNAGKLYATQFLMKKYNIPVKSILGFGDS
                     GNDEAYLSYLEHAYLMSNSRDEALKQKFRLTKYPYYQGITLHVKEFVEGKYDY"
     gene            5370..5795
                     /locus_tag="P6L01_001650"
     CDS             5370..5795
                     /locus_tag="P6L01_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDP3030719.1"
                     /translation="MKLKSFVTATLALGLLSTVGAALPSHEASADSNNGYKELTMDGK
                     HTVPYTISVDGITALHRTYFVFPENKKVLYQEIDSKVKNELASQRGVTTEKINNAQTA
                     TYTLTLNDGNKKVVNLKKNDDAKNSIDPSTIKQIQIVVK"
     gene            complement(6179..6883)
                     /gene="budA"
                     /locus_tag="P6L01_001651"
     CDS             complement(6179..6883)
                     /gene="budA"
                     /locus_tag="P6L01_001651"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDP3030720.1"
                     /translation="MTNVLYQHGTLGTLMAGLLKGTASINELLQHGDLGIATLTGSNG
                     EVIFLDGKAYHANEHKEFVELKGDELTPYATVTKFVADTSYETKDKSSEAVFAEIKEK
                     MLSENLFSAVKISGLFKKMHVRMMPAQEPPYTRLIDSARRQPEQTETYVKGSVVGFFT
                     PELFHGIGSAGFHVHFANDDRNFGGHVLDFEVEDVKVEIQNIETFEQHFPIQDKDFTK
                     ANIDYKDIADEIREAE"
     gene            complement(6920..8584)
                     /gene="alsS"
                     /locus_tag="P6L01_001652"
     CDS             complement(6920..8584)
                     /gene="alsS"
                     /locus_tag="P6L01_001652"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDP3030721.1"
                     /translation="MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGP
                     ELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGG
                     QVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
                     SIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEE
                     TNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTI
                     GYDPIEYEASNWNKELETQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISEKVDEP
                     FINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSH
                     YIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQ
                     DLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGL
                     RVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN"
     gene            complement(9078..9260)
                     /locus_tag="P6L01_001653"
     CDS             complement(9078..9260)
                     /locus_tag="P6L01_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030722.1"
                     /translation="MKDSIFWKKAFIPVYFIVAMLVFLLFRFYIKTDNFSIYLMIIFL
                     ICLGTASIIYNYKNNR"
BASE COUNT         3308 a         1641 c         1425 g         3319 t
ORIGIN      
        1 ttgtctttaa gttttcattt aattcttcga gttgcttatt taaaatttgc ttttgctgtg
       61 tttgaatatt tttattttta ggaagctgcg ctaatctaca aaattcgata agcgattcaa
      121 tactgacgcc tgcatttcgc aaatttttca ctaaataaat ccaatttaaa tcactatcat
      181 tatatattct atatccattt tcatctcgat taacgggtgg aatcacacca accttttcat
      241 aatatcttaa agtgtcttga gatatattca tgagcgctac gacttcttta gttttcattg
      301 cgactatcct ttcagttata tttgggcatc taaagtaatg ttgttttata tattgccatc
      361 tttgtttgaa tacttcttat tttattactc aaatttaaat ttgtctcttt tttaacattt
      421 tacatttcat cgtttttaat tactttaaaa attgtataac ttaaatattt aaaatgatat
      481 aatcactaag attgataata tttaattttt taaaaattat ttttgttgca atttttgggt
      541 gatacggaaa atttgggtga tcgatcgttt atatgcatca acattctttg tggattgttt
      601 gacagtagac agtcatttgt atacgttaac gaattttgaa aatttgaatc attatttaat
      661 catattatta attgcgcacg cacaacataa aaaggagaca tgtcaatgac atatagaatg
      721 aagaaatggc aaaaattatc caccattacg ttattaatgg ctggtgtgat tactttgaat
      781 ggtggtgaat tcagaagtat tgataaacat caaatcgctg tggctgatac gaatgttcaa
      841 acgccagatt atgaaaagtt gaggaacaca tggttagatg ttaactacgg ttatgataag
      901 tatgatgaga agaatgacgc aatgaagaag aagtttgatg ctacggagaa agaggcagag
      961 aaattactca gtagcatgaa aactgaaagt ggaaggactt acttgtggga tagtgcaaaa
     1021 gatttagata acaagtctgc ggatatgact cgtacctatc gtaatattga gaaaatcgca
     1081 gaagcgatga ggcatcctaa aaccacttta aatactgacg aaaataagaa gaaagtgaaa
     1141 gatgcccttg agtggctgca taaaaatgca tatggaaaag aaccagataa taaagtaaaa
     1201 gaattgactg agaattttaa aataactgat tcatcaaaga aaaaagcttt aaattggtgg
     1261 gattatgaaa ttggaacacc tagatcatta acaaatacgc ttatattgct gaatgatcaa
     1321 ttttcaaatg aagaaaagaa aaaattcact gcccctatta aaactttcgc cccaaaaagt
     1381 gatgaaatat tatcttctgt aggaaaagct gaacctgcta aaggcggaaa tttagtagac
     1441 attgctaaag taaaactttt agaaagtatt atcgaagaag ataaagatat gatgaaaaat
     1501 tcaatagatt catttaataa agtcttcact tacgttcaag attctgccac tgataaagaa
     1561 cgtaatggat tttataaaga tggttcatat atcgaccata aagatgtccc atacactggt
     1621 gcttatggcg ttgtactctt agaggggatt tctcaaatga tgccgatgat aaaagaaaca
     1681 ccttttaatg ataaaaccca aaataataca accttaaaat catggattga cgacggattt
     1741 ttaccactca tttataaagg tgaaatgatg gatttatcac gtggtagagc cattagccgt
     1801 gaaaatgaaa cgagtcactc agcatctgca actgtaatga aatcattgtt gagattaagt
     1861 gatgccatgg atgattcaac aaaagctaag tataaaaaaa ttgtcaaaac ttcagttaaa
     1921 tctgattcaa gttacggcca aaatgataca ttaagctctt attcggatat aagcaaaatg
     1981 aagtctttaa tggaagacag cactatttct actaacggtt taacacaaca acttaaaata
     2041 tataatgaca tggatcgtgt cacctatcac aataaagact tagactttgc atttggttta
     2101 agtatgacgt cgaaaaacgt cgcacgttac gaaagtatca acggagagaa cttaaaaggt
     2161 tggcacactg gtgctggaat gtcttattta tacaatagcg atgtgaaaca ctaccgtgat
     2221 aacttctggg cgacagctga tatgaaacgt ttagcaggta caacaacttt agaaaatgaa
     2281 gaacctaaag gtacggatgt taaaaagtcg agtaaaactt ttgtaggcgg aacaaaattc
     2341 gatgaccaac atgctagtat cggaatggat tttgaaaatc aggacaaaac tttaactgcc
     2401 aaaaaatcat atttcatatt aaacgataaa attgtcttct taggaactgg cattaaaagt
     2461 actgattcat caaagaatcc agttacaacg attgaaaatc gcaaagcgaa tgggtatacg
     2521 ttatatacag acgataaaca aacaaccgct tccgataatc agggaaccaa ttccgtcttt
     2581 ttagagtcaa ccaataaacc taaaaacaat ataggctatc attttttaaa cgaatcgaaa
     2641 ataactgtaa aaaaagaaag tcatactggt aagtggagtg atataaataa aagtcaaaag
     2701 caagacagta aaactaatca atattatgaa gtaactcaaa agcattctaa tacagatagt
     2761 aaatatgcat atgtgttgta tccaggctta tctaaagatg actttaatac aaaaaaagat
     2821 aaagtaactg tcgttaaaca agatgatgac ttccacgttg tgaaagataa tgaatcggtt
     2881 tgggctggtg ttaattatag taatagcact caaacttttg acattaacaa cactaaagtc
     2941 gaagttaaag ccaaaggaat gtttattctt aaaaacaaag atgataacac ttatgaatgt
     3001 agcttctata atcccgaatc tacaaatacc gcttcagata ttgaatctaa aatttcaata
     3061 actggatact ctattacaaa caaaaataca tcaacttcta atgaatccgg cgtacacttt
     3121 gaattaacta aataagcaag aaaatctcga cactatacca tttttatagt gtcgagattt
     3181 tataagtatc gccatcattc accttgtaat ccgcagacca catccccttt gataagttct
     3241 agattattta aaaatcgaat tttcaaagac gtacatgctt caatattctt atcattcatt
     3301 acttggaaat gtatatgagg ttcagtagaa ttgccagaat tcccaacctt cccaaggaag
     3361 tcaccatatt taacattttg cccctcattc acaatgattg aatattgttg taaatgagct
     3421 attaagctat actcattttc tgcgtgctta attacaatat aatttcctaa aaactgtgat
     3481 tcattcgtac ttccaatact gttgtcttga accccattca ctactttttc aactgtacca
     3541 tttgctggcg caatgatatc ttttccaaaa caaaaataat cagtatttac ttttccttga
     3601 ttcttatatg ttttatgatt ttctttaata actaaatcaa tagcatacct ttgatgtagg
     3661 tataaatagt gataattata taattcatta tctccacccc aaaaaacctc atagcaatct
     3721 gttgtcggca ctgcgtattt taaagtcgtt tcattcttaa tcgcatcata attgattaac
     3781 ggacgcataa caaatccgat aatactatta tctttccaag tcactgccac tccaaattga
     3841 tttgctttat ctatccatat ttgctccata gtatcatcat tttgagtaca cacttttaac
     3901 tcatgtacat cagactgcct tgtataagat ttgaaaagtt tcctaaactg ccaataacta
     3961 aactctcgct tcaaattttg atcaaaaatc ttatatattt tcctgctctt acttttattt
     4021 aaataatccc caaagtttgt tttattcata tatacttaac ctcttatatt aattttggta
     4081 tcaattcttt ctccagttcc tgcgtcattt ttgcgctacc tgaatcgtct acacttaatt
     4141 ggacaaatca acatataaat aattttttaa aactaaagtg tagtctattc accatatttt
     4201 ctaatcatcc cttaaatatt aatatttgca cttaaaaagt atacatatca ctcaactaat
     4261 caataatcat acttaccttc aacaaattct ttaacatgca gtgttatccc ttgataatat
     4321 ggatattttg ttaatctaaa tttctgcttt aatgcttcat ctctactatt cgacatcaaa
     4381 tatgcatgtt caagatagct taaatatgct tcgtcattgc cgctatctcc aaaacctaat
     4441 attgatttta ctggtatatt gtattttttc atcagaaatt gagtagcata taattttcca
     4501 gcatttgacg gtgtgaagtc aatatcatac gcattttcag gatcaccagc taatggattg
     4561 cttcgactaa atctcgctgt atagttttgg tcatttgcgt atctgacaag tgcttctagg
     4621 atacgactat cattgttgtg attacctgtt gaatgaaaat aaaaattata atgtgtttca
     4681 ttaccttcaa atgctctttg tggaataaat tcgacattat attgcgcaga aatatgacgt
     4741 aatatatctt caatatcttc aaataaaaat ggttcgctca agactttctt tttatatgtt
     4801 tctgattcaa catattcgcc attattccaa gtgaacattt tagaactcaa atcagaaaag
     4861 atatgtaatg gtttaaacgt catattaact tgatccattt tatccatgac actttgaaat
     4921 gtacttcctg tcaatacagc agtgatcact tcattattat tagttagttt ttccaacaat
     4981 ttttccattt ctcttaaatg acttaaatct tcttcatttg tattatgttt aaaatatgtt
     5041 tcatcaaaat caaacaataa tagacgcttt gacatactca ttcccccata atattctaat
     5101 gaatttatct ataacttaaa tacactataa gtctcaatta aaagtataag atcgactata
     5161 tgcttttcat gcttattata tacaactaat gaatccctat aaactaaaaa cattgcttac
     5221 cacttttcaa aatacacggg tcatatccct cgttaaaaac tattactaaa aatcaaaaat
     5281 ataaaaaaat caaaaagtat gttttaaact ttatgaaacg tgttatataa atagtgtaat
     5341 ataccacaac tttaaaagga gattgagata tgaaactaaa atcatttgtt actgccactt
     5401 tagcattggg attattatca acagtaggtg cagctttacc gagtcatgaa gcatctgcag
     5461 atagtaataa cggttataag gaattgacaa tggatggaaa acacactgtg ccttacacga
     5521 tctcagtaga tggtattact gcattacatc gaacttactt tgtttttcca gaaaataaaa
     5581 aggtacttta tcaagaaatt gacagtaaag taaaaaatga attagcttca caacgtggtg
     5641 ttacaacaga aaaaattaat aatgcacaaa cagcaacgta tacacttact ttgaatgatg
     5701 gtaataaaaa agtagtgaat ctaaagaaaa atgacgacgc taaaaattca attgatccaa
     5761 gtacaatcaa acaaatacaa attgtagtta aataataatt tgaaaatgaa aatagttaca
     5821 cagggcaaac gctgaaaaat tgcgtttgtc tttttagtat gaaatgaatg atcgaactta
     5881 taatttctta ttgattgctt ctactgacaa tagattagat cggcctatac taaagtaaat
     5941 tctatattaa tgtcttccgc aatttataat ctaacataac agcaaatctc aaatatctgg
     6001 aacctaataa atcccctcac tactgctaat agtgaaggga tttattttat tacttagacg
     6061 taattataaa tatatttgtt ctattgtagt catggttaat catatttaca aactatcatt
     6121 tctaaatcgc gcattatgaa gtcaattttc ataaaccgtc attatatttc tggattcatt
     6181 attcagcttc tctaatttcg tctgcaatat ctttatagtc aatatttgct ttagtgaaat
     6241 ctttatcttg aattggaaaa tgctgttcaa atgtttctat attttggatt tctactttaa
     6301 catcttctac ttcaaaatct aagacatgtc caccaaagtt acgatcatca ttcgcaaagt
     6361 gtacatgaaa tcctgctgat ccgataccat ggaataattc tggtgtaaag aaaccaacga
     6421 ctgaaccttt gacatacgtt tcagtttgtt caggttgtct tctagctgaa tcgattaaac
     6481 gtgtataagg tggttcttga gccggcatca tacgtacatg cattttttta aataagcctg
     6541 aaatttttac tgctgaaaat aaattttcac tcaacatctt ttccttaatt tctgcaaaaa
     6601 ctgcttctga agatttatct ttcgtttcat agcttgtatc tgctacaaat ttagttacag
     6661 ttgcatatgg tgttaactcg tcacctttta attctacaaa ttctttatgt tcatttgcat
     6721 ggtaagcttt tccatctaaa aagattacct caccgtttga acctgttagt gtagcgatac
     6781 ctaagtcacc atgttgcaat aattcattta ttgatgcagt tccttttaat aatcctgcca
     6841 ttaatgtgcc taatgtacca tgttggtata aaacattagt cataaaatct cccactcctc
     6901 tttaacattt atctttattt tagttaaata cgtcaggtaa catatttgtt gaaagtttga
     6961 tattatcttt gtaatttaca ggtatatcaa ttaatactgg accatctgtt tcatagccct
     7021 ctttaattgc cgcttctaat tcttcttgat tagtaactcg taaacctttc gcgccaaatg
     7081 attctgcata ttttacaaaa tctacaggac cgaagtctac acctgacgaa cgtttatatt
     7141 tcatttcttc ttggaattca accatgttat attttccatc attccaaata agctggatga
     7201 tatttaaatt tttacgtacg gccgtttcta aatcttgtga tgaaaataaa aagccaccat
     7261 cgccagcaac ggacacaact tgcgtattag ggcgcacaag tgcagctgaa attgcccacg
     7321 gtaatgctac accaagcgtt tgcataccat tgctaaataa taaatgtctt ggattgtaac
     7381 ttctgaaatt acgtgccatc caaatatagt gacttccaac atcaactgtt acagtagtat
     7441 catcagttaa taccttttgc atagattcaa taatttccac tggatgtaga attccttctt
     7501 catgcgtcgc tttaatacca gtttcttcat caatatgtgt tcttaattgt tctaattcgt
     7561 ctaaatgttg ttgatttata aatggctcat ccactttttc agaaatcatt tcaatcgttt
     7621 tagcaatatt accaatcaac tcttttttcg gttgcatata attagtaatt tcagcttgaa
     7681 cttcgtcaat attgataatt tgtgtttcta attctttatt ccagttacta gcttcgtatt
     7741 caattggatc ataaccgatt gtaacaacta aatcactttt acgtaataat tcatcaccaa
     7801 cttgattgcg gaataagccc acacgaccga agaaatgatt ttctaattca cgactaatta
     7861 cacctgcacc ttggaatgtt tctacaactg gtaaattcgt gcgctcaact aatttgcgaa
     7921 tggcatttgt ttcttctgca cttgaacttc tcataccagc taataaaaca ggaaatgatg
     7981 catttttaat cgcttcaatg acatcattaa tatcttgttc actcggtact cctaaatttg
     8041 gtttttggca aagtgatata gctttagatt caactggtga agaaataacg tcttgcggaa
     8101 tactaataaa acttgcgcca ttttttcctg aagtagcaat tcgaattgca tttgtcataa
     8161 cttctgataa tgattcagga tcttgtactt cttcactgta ttttgatgaa tattttaata
     8221 gcgcagcatt atcaatactt tgatgcgtta atcgtaataa atcattacgt ttcacttggc
     8281 cacctaacgc taatacagga tccccttcag atgtagctgt taatagtcca gtcgttaaat
     8341 tacttacacc agggccactt gtaacaagta ctacacccgg tttacctgtt aatcttccaa
     8401 taccttgtgc catcattgca gcattttgtt catgacgagt gacaataagt tcaggaccat
     8461 catcaattaa agcattaaat agatagtcta tctttgcacc cggaatacca aaaacatatt
     8521 ctaccccatt atttttcaaa gtatcaataa ccatatcggc tgcagtgtac tttttatcag
     8581 tcatttatat tcatttcctt ccaattgtga tgtatagatg tgtattacat gtaattacat
     8641 tgtgatttgt aatctaatga tagttacgca aaattaatga tgtttatcaa aattttacat
     8701 gtacgattag atttgtaata cataaaaatt gccttcatta cattatataa cctttgttag
     8761 atgaataaaa atcgttattt gtaatactaa gtattccaaa aatgcatgag tagattttgt
     8821 gcgttgtgat tatgagggat gttggattga ttgatgtata ggattgttac gatattttgg
     8881 gatgaggtgt ttagtcaatg gcggtttgaa attgtcgttt tatggtgggc tgaaaatgct
     8941 gttttatgtc gggcttgaat ttgtcgtttt atggagatat aaaaatgctg ttttatggcg
     9001 gtttcaaaat ggtgtttcga ttaataagcc acagcaagac ttagatctaa ctatgactta
     9061 tacatttcac acttatatta gcgattattt ttatagttat aaatgataga tgcagttcct
     9121 aaacaaatta agaagataat cattaagtat atagaaaagt tatctgtttt aatataaaac
     9181 ctaaaaagta gaaacaccag catcgcaaca ataaaataaa caggaataaa agctttcttc
     9241 caaaatatag aatctttcaa ttaggtcact ccttattttg ataatactct ttaattaagg
     9301 ctcttgagtt atcgtccggt cgttctttcc cttgaaccac tatgattaga acacaatcgt
     9361 ctggttatcg tccaccctcc gcaagctaaa tgacaattta ataatgtaca cttttgattg
     9421 tgtaaacatg tacaaattat acgtgtaaat caataatcca tagttgaatt aattctattc
     9481 ctaagacatt aaataaagga aagtccattt atactctaca catttcatat tccccacaca
     9541 aacgcaaaaa aacacctcga cataacatcg aggtgttttg tatatatcgt ccgacaatta
     9601 acgttttaag aaatgaggtg aacgacgagc tgctttaaga cctggtttac tttaggattt
     9661 cgtatagttg catatactgt cttaaggcat gat
//
LOCUS       DAOBBH010000096         9691 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.096, whole genome shotgun sequence.
ACCESSION   DAOBBH010000096 DAOBBH010000000
VERSION     DAOBBH010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9691
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.096"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            37..654
                     /locus_tag="P6L01_001654"
     CDS             37..654
                     /locus_tag="P6L01_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HDP3030723.1"
                     /translation="MVTAIIHGFILAIGLILPLGAQNVFIFNQGANQPKYRYVLPAII
                     TAGLSDSLLIIIAVVGVSIIIMSLPVLQAIIYIVGLIFLMYMAWTIWHDKPSTDGEAQ
                     IISPMKQVSFALSVSLLNPHAILDTIGVIGSSAALYSGSNKIAFTIACISVSWLWFFL
                     LAILGKMVGSIDKTGKLLTIINKISSIIIIIVALMILQKLIQLLF"
     gene            complement(727..1308)
                     /locus_tag="P6L01_001655"
     CDS             complement(727..1308)
                     /locus_tag="P6L01_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDP3030724.1"
                     /translation="MAKTLYLMRHGQTLFNFKGLIQGFGDSPLTELGIAQAQKARSYY
                     ETKGINFDLYASSTQERASDTLENVAPNQSYQRFKGLKEWHFGIFEGESVYLFDNLYK
                     PEDLFGDRIVPFKGEARKQVEERIVKTLHDIMSQTKNNALVVSHGTIMGVFLRYCLKL
                     DEALKHNIGNCNILKFEYDNGTFKFVELIDPNL"
     gene            1533..1769
                     /locus_tag="P6L01_001656"
     CDS             1533..1769
                     /locus_tag="P6L01_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030725.1"
                     /translation="MDHHNQCNYVNPQNVSLDWECFIISKSEMLLDGVPNELINTWLD
                     KDIITPFSIRNDEINFKTKDIWDALIHHNWYYSN"
     gene            complement(1766..1969)
                     /locus_tag="P6L01_001657"
     CDS             complement(1766..1969)
                     /locus_tag="P6L01_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HDP3030726.1"
                     /translation="MTFYNFIMGFQNDNTPFGILAEHVSEDKAFPRLEERHQVIRAYV
                     MSNYTDHQLIETTNRAISLYMAN"
     gene            2310..2570
                     /locus_tag="P6L01_001658"
     CDS             2310..2570
                     /locus_tag="P6L01_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030727.1"
                     /translation="MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICG
                     VILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPIFYSFIG"
     gene            2599..2787
                     /locus_tag="P6L01_001659"
     CDS             2599..2787
                     /locus_tag="P6L01_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030728.1"
                     /translation="MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVI
                     LYIFGFAILIYTFIFGVL"
     gene            2984..3544
                     /locus_tag="P6L01_001660"
     CDS             2984..3544
                     /locus_tag="P6L01_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030729.1"
                     /translation="MKRTIILLILFVVVSPINSPKTIADNKYSEIQDDKFQLQPGDII
                     VTKGPVMWGFFGHCSIAIDDKTILQIEGPGDKPTTQSFESFKYNYASGKNDWMKVYRC
                     SYPGAGKKAAEWVKKNYENTNHRYLVTLNLNSKKFTYCTKIIYQAYKFGVSEKSVKSY
                     GLHIISPYAIKDNFIDPYKLRLVKAY"
     gene            3632..3916
                     /locus_tag="P6L01_001661"
     CDS             3632..3916
                     /locus_tag="P6L01_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030730.1"
                     /translation="MNELTKEQKYTIAKFYKLYIERSNNGETETVANFFGDAKDAREN
                     YFCDRDYQDFLTNCQILIQNKYLTGEILDDNIYNISILNKTFIEFEQNFG"
     gene            3978..4196
                     /locus_tag="P6L01_001662"
     CDS             3978..4196
                     /locus_tag="P6L01_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030731.1"
                     /translation="MSEWHINNQSTKPFLIQQAEKQLSIVKPLPNGSFQILTEINLEN
                     GNISNIDHSLLVSVDPKALEISIFDAVN"
     gene            complement(4688..4888)
                     /locus_tag="P6L01_001663"
     CDS             complement(4688..4888)
                     /locus_tag="P6L01_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDP3030732.1"
                     /translation="MNNGTVKWFNAEKGFGFIEREDGSDVFVHFSAIAEDGYKSLEEG
                     QKVEFDIVEGDRGEQAANVVKM"
     gene            complement(5245..5931)
                     /locus_tag="P6L01_001664"
     CDS             complement(5245..5931)
                     /locus_tag="P6L01_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDP3030733.1"
                     /translation="MTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFFFATSCL
                     VLTLVVVSSLSSSANASQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTV
                     KLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENANKIEVEFDKGQR
                     TDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNI
                     WSEDNADSGQ"
     gene            complement(6272..6793)
                     /locus_tag="P6L01_001665"
     CDS             complement(6272..6793)
                     /locus_tag="P6L01_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001627793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030734.1"
                     /translation="MKRKVLVLTMGVICATQLWHSNHANALVTESVETNFVVKDSDNK
                     NILQTHTEITTEEKFSIVEKSQLNKLKALSNDNYIEYDLHTNQTGIKKGWLYGYSEID
                     SSHFTDRDKRVIRRDHVKEAQSLINDYKDTQSYEDLAKATAKVSTLSQSHQNYLNKQI
                     DKVNNKIEKTEKR"
     gene            complement(7128..8150)
                     /gene="emp"
                     /locus_tag="P6L01_001666"
     CDS             complement(7128..8150)
                     /gene="emp"
                     /locus_tag="P6L01_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix protein-binding adhesin
                     Emp"
                     /protein_id="HDP3030735.1"
                     /translation="MKKKLLVLTMSTLFATQLINSNHAKASVTESVDKKFVVPESGIN
                     KIIPAYDEFKNSPKVNVSNLTDNKNFVVSEDKLNKIVDSSAASKIVDKNFAVPESKLG
                     NIVPEYKEINNRVNVATNNPASQQVDKHFVAKGPEVNRFITQNKVNHHFITTQTHYKK
                     VITSYKSTHVHKHVNHAKDSINKHFIVKPSESPRYTHPSQSLIIKHHFAVPGYHAHKF
                     VTPGHASIKINHFCVVPQINSFKVIPPYGHNSHRMHVPSFQNNTTATHQNAKVNKAYD
                     YKYFYSYKVVKGVKKYFSFSQSNGYKIGKPSLNIKNVNYQYAVPSYSPTHYVPEFKGS
                     LPAPRV"
     gene            complement(8503..>9691)
                     /gene="vwb"
                     /locus_tag="P6L01_001667"
     CDS             complement(8503..>9691)
                     /gene="vwb"
                     /locus_tag="P6L01_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001628779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="von Willebrand factor binding protein Vwb"
                     /protein_id="HDP3030736.1"
                     /translation="KIKNADISRKSNDLLGLTHERYSYIFDTLKKNKQEFLKDIEEIQ
                     LKNSDLKDFNNTEQHNADVEINNLENKVLMVGYTFYNTNKDEVEELYSELDLIVGEVQ
                     DKSDKKRAVNQRMLNRKKEDLEFIIDKFFKKIQQERPESIPALTSEKNHNQTMALKLK
                     ADTEAAKNDVSKRSKRSLNTQNNKSTTQEISEEQKAEYQRKSEALKERFINRQKSKNE
                     SVVSLIDDEDDNENDRQLVVSAPSKKPTTPTTYTETTTQVPMPTVERQTQQQIVYKTP
                     KPLAGLNGESHDFTTTHQSPTTSNHTHNNVVEFEETSALPGRKSGSLVGISQIDSSHL
                     TEREKRVIKREHVREAQKLVDNYKDTHSYKDRLNAQQKVNTLSEGHQKRFNKQINKVY
                     NGK"
BASE COUNT         3084 a         1477 c         1360 g         3770 t
ORIGIN      
        1 tcagaatatt ctatgtttta ggggtgtcaa aacttaatgg taaccgcaat tattcatgga
       61 tttatcttag cgattggtct aattttacct ttaggtgcac aaaatgtatt tatttttaac
      121 caaggagcta atcaaccaaa atatagatat gtattgcctg caataattac agccgggttg
      181 tcagacagct tacttattat tattgcagtg gtaggagtat ctatcattat tatgtcttta
      241 cctgtacttc aagcaattat ttatatagtt ggtttaattt tcttgatgta tatggcttgg
      301 accatttggc atgataaacc ctcaacagat ggagaagctc aaattatatc tccaatgaaa
      361 caagtaagtt ttgctttatc agtttcatta ctcaatccac atgctatttt agatacaatt
      421 ggtgtaattg gtagtagtgc tgcattatat agtggcagca ataaaattgc gtttacaatc
      481 gcttgtatta gcgtttcatg gttatggttc tttttactcg caattttagg aaaaatggtt
      541 ggatcaattg ataaaactgg aaaattatta acaataatca ataaaatatc aagcataata
      601 attattattg ttgctctaat gatattacaa aaacttattc aattattatt ctaaagcgtt
      661 atgtagttgt attttaaaag cgttatacaa aagacatact tgtgtctatg tataacgctt
      721 tatttattat aaatttggat caattaactc aacaaattta aatgttccat tgtcatattc
      781 aaatttcagg atattacaat taccgatatt atgctttaat gcttcatcta gtttaaggca
      841 atatcttaaa aatactccca ttattgttcc atgactcacg actaatgcat tattctttgt
      901 ttgagacata atgtcatgta aagttttcac aatgcgttct tcaacttgtt tccttgcctc
      961 tcctttgaaa ggaacaattc gatctccgaa taagtcctca ggcttgtata gattatcaaa
     1021 tagatagact gactcacctt caaatattcc aaaatgccat tccttcagtc ccttaaaacg
     1081 ttgatacgat tggttaggtg caacattttc aagtgtgtca cttgcgcgtt cttgcgttga
     1141 tgatgcatat aaatcgaagt ttatcccctt agtttcataa taactacgtg ccttttgagc
     1201 ttgagcaatt ccaagttctg ttagcggcga atctccaaat ccctgaatta gtcccttaaa
     1261 attaaacaaa gtttgtccgt ggcgcattaa atataacgtt ttagccaaca ataacacctc
     1321 ttcaataaaa agtattgata gctcaaaatg tattctcttt ttattaaaat gagtaccaaa
     1381 tatattaaac cattatttaa actttttcgg tagcgaaaac ctttgctttt gatgtacatt
     1441 tttttcataa gtaatgatga ttttagttta aaagataatg tgacggggta aaacgcaatg
     1501 aagagtacag aattttaaag gagtgagtgt ttatggatca tcataatcaa tgtaattatg
     1561 tgaatccaca aaatgtttca cttgactggg aatgttttat aattagtaaa agtgaaatgt
     1621 tgttagatgg tgtaccaaat gaacttatca acacttggtt agataaagac attattacac
     1681 cattttcgat aagaaatgat gaaataaact ttaaaacaaa agatatttgg gatgcactaa
     1741 tacatcataa ttggtactac tcaaattaat ttgccatata taagctaata gctctatttg
     1801 tagtttcaat taattgatga tctgtgtaat tagacatcac atatgctcta attacttggt
     1861 gtctttcttc taatcgaggg aatgctttat cttcactaac gtgttcggcc aatataccaa
     1921 atggtgtgtt atcattttga aaacccatga tgaaattgta aaacgtcata agacttgccc
     1981 ccttcattta ttaagcgcta tttaaagtgt tattattaat cttatcagaa aactagtatt
     2041 tgtaaatgtg caaaagtgat tttgcacatt tttttgatat ctgattctaa aaatattaac
     2101 gaattataca actttaagag tttttagtat gtaaatattc ttttcatatt aatactatac
     2161 atccctatat atgctcactt gtttgaatta ataaacttta gcattctaca aataatattt
     2221 taaaaataac tatacttttt atttacctat tggaaaatag tgttatcata tttattaaga
     2281 agctattttt atactagtag gtgtcagtca tgaataacat tttgttaaat gctatcaata
     2341 tagttattac taccactttt gttatcttta atattttaat cacatataat aaagatttag
     2401 atgatttatg ttggctcctg cctggtatta tcatttgtgg tgtgatactc atcgtatcct
     2461 ttaccattgc aatgataact aaaaactggt taagtgaaat attatttttt ataaatatcg
     2521 tactcgttct ctattacatt tatcctattt tttatagttt tataggttaa taattacggt
     2581 atataaggag ggtttcacat gtctttacat tttgcaattc tgttttggct agcattaatt
     2641 ttcttagttg ccgctacgtt tatactcgta ttaatgaaaa aaactggcaa agaatctaaa
     2701 aaagagtcct atttaagttt cactgtcatt ctctatattt ttggattcgc tatattaata
     2761 tacacattta tatttggtgt gctataaatg aaattcattt tatatccagt ctgaaacgtc
     2821 tcaatgatgt ttcagactgt tttttattct taatagacat ttcacactct catactttaa
     2881 tatttaagat atgttttagt cttacaagct tattaaagat tacaattcta atttaagact
     2941 atttacatat atttcttcaa caaggagctt ttaagcgaac tatattaaac gaacgataat
     3001 tttacttata ttattcgttg tcgtatcccc tataaactca ccaaaaacaa ttgcagataa
     3061 taagtattca gaaattcaag atgacaaatt ccaattacaa cctggtgata ttattgttac
     3121 aaaaggccct gtcatgtggg gattttttgg tcattgtagt atcgcgattg atgataaaac
     3181 gattttacaa attgaggggc ctggcgacaa accaactaca caatccttcg aatcttttaa
     3241 atataattat gcaagtggca aaaatgattg gatgaaagtt tatcgttgca gctatcctgg
     3301 tgcaggtaaa aaagcggctg aatgggttaa gaaaaactat gaaaatacaa atcatcgtta
     3361 tctagttaca ttaaatttga acagcaaaaa atttacgtat tgtactaaaa ttatctacca
     3421 agcttataaa tttggtgtaa gtgaaaaatc agttaaaagt tatggactac atattatttc
     3481 accctatgca attaaagata attttataga tccatacaaa ctaaggcttg taaaagctta
     3541 ttaacacagt ttcatcaaat agttaaatta gtttgtgttt tgcaataaat tgggtataga
     3601 ttacaaatga tatttcagga ggcccaatat tttgaatgaa ctaactaaag aacaaaagta
     3661 tacaatagct aaattttaca aattatatat tgaacgttcg aataatggag aaacagaaac
     3721 agtcgctaac ttttttggcg atgctaaaga tgcacgtgaa aactattttt gcgatcgtga
     3781 ttatcaagac ttcttaacta actgccaaat tttaattcaa aacaaatact taactggtga
     3841 aattttggac gataacattt acaatatttc tattttaaac aagacattta ttgaatttga
     3901 acaaaatttt ggttgaacat gaatgtctca actataaact ttttaaccct attttaaagc
     3961 aggagtgaaa aacatacatg agtgaatggc atattaataa tcaatctaca aaaccttttt
     4021 taatacaaca agcggaaaag caattgtcta ttgttaaacc attgcctaat ggtagcttcc
     4081 aaatactcac agaaataaat ttggagaatg gcaatattag taacatcgat cattctttac
     4141 ttgtttcagt agaccccaaa gcattagaaa taagtatatt tgacgctgta aattaaacgc
     4201 aatattttta tttaattatt ttaatgtttc gccttttaaa tgaaaacaag attactgaat
     4261 tattatgaga ttttaaaaac aataaatatt ctcaataatt aatcagtctt aataaaaaat
     4321 taacaaaaag ttgattagtt aaattattaa ttaagtagta agatattatg cgatgtggta
     4381 tcctattatt gttaaacatt ttgagtgttt aacattactt cttgttatcg ccatatacag
     4441 actttaattc tcgtcattat taaagttctg tattggcgct ttttttattc aaaatcaaaa
     4501 gtcggacaga tgaaaagtaa aaaacttttc catcaattcg atttattata gaaccattat
     4561 atattgttag atctccacaa tttattggac aaaaaaataa cggctagtgc ttttaccaca
     4621 ccaactgtta tcgatttaaa gcataaaaaa acagccctat aataaggact gtttgaataa
     4681 gttaaaatta cattttaact acgtttgcag cttgctcgcc acggtcgcct tcaacgatgt
     4741 cgaattcaac tttttggcct tcttctaatg atttgtatcc atcttcagcg attgctgaga
     4801 agtgtacgaa tacgtcgcta ccatcttctc tttcgatgaa accaaaacct ttttctgcat
     4861 taaaccattt aactgtaccg ttattcatat tgaatacctc cgtcgtgctt ttgcactaaa
     4921 tattttgtaa caaattcata aatgaaaagg agaatattct atataaataa ctcacatttt
     4981 tcttcacgct ctttattact taactttcat tatacacttt taaatataga agtaaagcat
     5041 tattttattt tctgtgataa tacacattga actatatagt aacattccat tttaatacta
     5101 aatttaaaat ttttattttc taatattaaa tacacttgct tttgatacta tttacttttt
     5161 taattctgaa ttgataataa atcatatcgt gatctaaaaa ttataaaagg gccactagca
     5221 gcagtgacac ttttacaatg agcattattg acctgaatca gcgttgtctt cgctccaaat
     5281 atttaatttc tcttttttcg cttgtgcttc actttttctt aaaagttgtt catgtgtatt
     5341 gttaggttta taaacataag caactttagc caagccttga cgaactaaag cttcgtttac
     5401 catttttcca tcagcataaa tatacgctaa gccacgtcca tatttatcag ttctttgacc
     5461 tttgtcaaac tcgacttcaa ttttatttgc attttctacc atttttttcg taaatgcact
     5521 tgcttcaggg ccatatttct ctacaccttt tttaggatgc tttgtttcag gtgtatcaac
     5581 taataatagt ctgaatgtca ttggttgacc tttgtacatt aatttaaccg tatcaccatc
     5641 aatcgcttta attaatgtcg caggttcttt atgtaatttt ttagttgaag ttgcactata
     5701 tactgttgga tcttcagaac cacttctatt tacgccgtta tctgtttgtg atgcatttgc
     5761 tgagctactt agacttgaaa ctacaactaa agttaacact aagcaactag tagcgaaaaa
     5821 gaaaaacctc tttgcgtatt gccctttcga aacattactg atagccatcc ctataagtaa
     5881 tattgaaaca attgccatac atatgccagc acttaataag tattctgtca taactaacac
     5941 ctctttcttt ttagttaatt ttaatattaa acgttaatta attataattc tacattttta
     6001 caattaatta ataaaaattt aataattata ataaaataac tatacttact tttatgtatt
     6061 ttctatattt taggtgtcag aacttatata tttagcctta tttgtaaaat taaaaagcct
     6121 acaacatgaa tcgttgcaga ctatcatatc gagacaaaga ggtttattag cgtctctata
     6181 tctatgcgga atttgaaaag attatttatt atatttacgg ggataaattg tcatttaaaa
     6241 acgagatgta taacttataa tttactttgg attagcgttt ttcagttttc tctattttat
     6301 tattcacttt atcaatttgt ttatttaaat aattttggtg agactgacta agtgtactta
     6361 cttttgcagt tgccttagcg agatcttcat agctttgtgt atctttataa tcgttaatta
     6421 agctttgtgc ttctttaaca tgatcacgtc taataacgcg tttgtcacgg tctgtgaaat
     6481 gtgatgagtc aatttcacta taaccatata accaaccttt tttaatacct gtttgattag
     6541 tatgtaaatc atattctatg tagttgtcat tagatagtgc ttttaattta tttaattgac
     6601 tcttttctac tattgaaaat ttctcctcag tagtaatttc agtatgggtt tgtaaaatat
     6661 ttttattatc cgaatctttt acaacaaaat tagtttcaac actctctgtc actaatgcgt
     6721 ttgcgtgatt agaatgccat aattgagttg cacaaattac acccattgtt agtacaagta
     6781 ctttcctttt catttattta tgctccctta tttgctttga ttgtaaaacc gactttttca
     6841 ccctgttttc ttcgccattg aaaattatat accaatattt tagaaacaat aattaacttg
     6901 cgatgaacaa actattaaca ataatcttga gtcttatatt tatcttaatt aataatatat
     6961 ttatttaaga ttatgttact tccaactttc aaagtagaaa aacggtataa tttgttgatg
     7021 ggtgtttatt gataaactgc aaaaaataca gcctacaacc acatagattg tagactatat
     7081 ttaaaataat aggtatttat catatctcgt aaacttagtg ccaattttta tactcgtggt
     7141 gctggtaagc tacccttaaa ttcaggaacg tagtgtgtag ggctataact tggaacagca
     7201 tattgataat ttacattttt gatatttaat gatggtttcc caattttata accatttgat
     7261 tgtgaaaatg agaaatattt cttcacacct ttaactactt tataagaata gaagtattta
     7321 tagtcatatg ctttatttac tttagcattt tgatgtgttg ctgttgtgtt attttggaaa
     7381 cttggtacat gcatacgatg tgaattgtga ccatatggtg gaattacctt gaaactattt
     7441 atttgtggca caacacaaaa gtgattaatt ttaatgctag catgccctgg tgtaacaaat
     7501 ttatgcgcgt gatatccagg aactgcaaaa tgatgcttga taattaaaga ttgagatgga
     7561 tgtgtatatc taggcgattc tgatggttta acaataaagt gtttattaat agaatccttt
     7621 gcatgattta catgtttatg tacatgtgtt gatttgtatg aagtaataac tttcttgtag
     7681 tgggtttgcg tagtaatgaa gtggtggttt actttgtttt gcgtaataaa tctatttact
     7741 tctgggcctt tagcaacaaa atgcttatca acttgttgtg aagctggatt gtttgttgct
     7801 acattcacgc gattattgat ttctttgtac tctggtacaa tgtttcctaa ctttgattct
     7861 ggtacggcaa agtttttatc tacaatttta ctagctgccg atgaatctac aatcttattc
     7921 aatttgtctt cagaaactac aaagttttta ttgtcagtta aattactaac atttactttt
     7981 ggcgaattct taaattcatc gtaagctgga ataattttat taattcctga ttctggaact
     8041 acaaattttt tgtcaacact ctctgtcact gatgctttag cgtgatttga attgataagt
     8101 tgtgtagcaa atagcgtgct catagttaaa actaataatt tctttttcat ctgttatttc
     8161 tcctttatat agactcaata ttataaccaa tataatttcc ctgttatatt cactaacagc
     8221 attatatacc agaattttca gtataataat taacttgaag taaacgttgt cttaacattt
     8281 ttattgtttt tcagcttaaa attaattatt gatattgata gttaagcata ataatttttt
     8341 cgtaatataa agtgaaaaaa gtaatagtcc acacctgttt agaatgtgga ctatactaga
     8401 ttgcatcatt gaaatgatga ctttgatatt atttattgct agtttaaaat gttgttattt
     8461 ttacgacaaa ctcattattt gctttgcagc catgcattaa tattatttgc cattgtatac
     8521 tttattgatt tgtttattaa aacgtttttg atgaccttca cttaaagtat ttactttttg
     8581 ttgtgcattt aatcggtctt tataactatg tgtatcttta taattatcaa ctaacttttg
     8641 agcttctcta acgtgttcac gcttgattac acgcttctca cgttcagtta gatgagaaga
     8701 atcaatttga cttataccaa ccagtgatcc tgattttcta ccaggtaaag cagacgtttc
     8761 ttcaaattca acaacattat tatgcgtatg atttgaagtt gttggtgatt gatgcgttgt
     8821 tgtgaaatca tgactttcac catttaatcc agctaatggt tttggtgttt tgtaaacgat
     8881 ttgttgctga gtttgacgct caactgtagg cattggtacc tgagtcgttg tttcagtata
     8941 tgtagtcggt gttgttggtt tctttgatgg cgcagaaacc acaagttgcc tgtcgttttc
     9001 gttgtcgtct tcgtcatcga ttagtgaaac cacagactca tttttagatt tttgtctgtt
     9061 tataaatctt tcttttaatg cctctgactt tctttgatat tcagcttttt gttcttcaga
     9121 aatttcttgt gttgtagatt tattattttg agtatttaaa cttcttttac ttctttttga
     9181 tacgtcattt ttagcagctt ctgtatctgc ttttaacttt aatgccatag tctgattatg
     9241 atttttttca ctagttaatg ctggtatact ctctggacgt tcttgttgaa tttttttaaa
     9301 aaatttatct ataataaatt ctaaatcctc tttttttcta tttaacatcc tttgatttac
     9361 tgctcttttt ttatccgact tatcttgaac ttctccaaca atcaaatcta actcactata
     9421 taattcttca acttcgtcct tatttgtatt atagaatgta taccctacca ttaatacttt
     9481 attttctaaa ttgtttattt ctacgtcggc attatgttgc tctgtattgt taaagtcctt
     9541 taaatcacta tttttcagtt gtatttcttc aatatctttt aaaaactctt gtttattttt
     9601 ctttaatgta tcaaaaatat aagaatatct ttcatgtgtt aaacctaata aatcattcga
     9661 ttttctgcta atatctgcat tttttatctt c
//
LOCUS       DAOBBH010000097         9470 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.097, whole genome shotgun sequence.
ACCESSION   DAOBBH010000097 DAOBBH010000000
VERSION     DAOBBH010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9470
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.097"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(94..846)
                     /locus_tag="P6L01_001668"
     CDS             complement(94..846)
                     /locus_tag="P6L01_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDP3030737.1"
                     /translation="MQALQTKSNIGEMFNIQEKENGEIAISGRELHQALEVKTPYKKW
                     FERMSDYGFEENIDYIVTDIFVHNPLGGRQNQTDHALTLDTAKEIAMIQRSEPGKRAR
                     QYFIQVEKAWNSPEMIMQRALKIANNTINQLETKIERDKPKIVFADAVATTKTSILVG
                     ELAKIIKQNGINIGQRRLFEWLRQNGFLIKRKGVDYNMPTQYSMERELFEIKETSITH
                     SDGHTSISKTPKVTGKGQQYFVNKFLGEKQTT"
     gene            897..1226
                     /locus_tag="P6L01_001669"
     CDS             897..1226
                     /locus_tag="P6L01_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030738.1"
                     /translation="MTDEAKFVLLQLYSIYLDRIDEGMSKRSASYFGSDESSFNAFFL
                     GFNFEDYIDAVLELKHRDFVIASAEDGGFLEMALSREGIAYSESESKKDYKTLMGLIR
                     DLKKLII"
     gene            complement(1215..1430)
                     /locus_tag="P6L01_001670"
     CDS             complement(1215..1430)
                     /locus_tag="P6L01_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDP3030739.1"
                     /translation="MNIQEATKLAMEKGISIRRENQDVYGILPTNLQRYQCLVVSRHY
                     KKKRQTAAGRWQPSADDLIADDWILDY"
     gene            complement(1446..1709)
                     /locus_tag="P6L01_001671"
                     /pseudo
     CDS             complement(1446..1709)
                     /locus_tag="P6L01_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500089.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(1706..1879)
                     /locus_tag="P6L01_001672"
     CDS             complement(1706..1879)
                     /locus_tag="P6L01_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDP3030740.1"
                     /translation="MFLAIIIFLSLFILLTSFFLIIRNFHIIVKFFTKKTYLMLTIRK
                     FRIILGLRKAVTT"
     gene            1842..2555
                     /locus_tag="P6L01_001673"
     CDS             1842..2555
                     /locus_tag="P6L01_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDP3030741.1"
                     /translation="MNKERNIIIAKNIRKFLNDSNMSQKKLAELINIKPSTLSDYLNL
                     RSNPSHGVIQRIADVFEVGKSDIDTTYKDDNDITSIYNKLTPPRQENVLNYANEQLEE
                     QNSKGDNVVDINSYKQEKTPVNVNGCVSAGVGERLHDETLFTEMVKGPIPTHDLALKV
                     NGDSMEPMFKDGEIIFVEKTHNIKNGQIGIFIIEEEAYVKKVFVEDDRLTLVSLNKDY
                     DDLHFYRNESVRLIGKVIL"
     gene            2571..3503
                     /locus_tag="P6L01_001674"
     CDS             2571..3503
                     /locus_tag="P6L01_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDP3030742.1"
                     /translation="MKKYDIAVLDFETMNEHMNSPCEVAVSLIKDLSIVKVYSSYINP
                     PNNRYNLKNAKIHKIPEDVILKAPKYPDIYQEILYLLKESHLIIAHNALFDISVLKNT
                     NNYYDLPVPNFMYVDSINIFRSFHAISSFKLENLCSLYDIDKEKLHSAKFDVLALSKM
                     LISLAKNNQHYSVLKLIHYMPKQYIRFSKYSNSPTKLFDSGFQKIHMKISEINKIEVE
                     SVIPILKDKNVVFTGNFDTEKQDLMILTRKKGAYIRSDVTAKTDILVEGVQDDKYKDV
                     NGLVSKQRKAREYVGNGAKIQFLNEEDLINLIKE"
     gene            3509..3850
                     /locus_tag="P6L01_001675"
     CDS             3509..3850
                     /locus_tag="P6L01_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030743.1"
                     /translation="MIKKIFTKKHVFLVIEDENHNHSDAVFGKSILLSIYVGVNKKTN
                     SKSGKFIYLDRSKRIVRQSDITKIESANENDVDFYNLLKKEKEIVYSKNIVDKYNLAN
                     YIIYYEVSTKE"
     gene            4054..4236
                     /locus_tag="P6L01_001676"
     CDS             4054..4236
                     /locus_tag="P6L01_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030744.1"
                     /translation="MKITNCKIKRETVIYEVLTSGNQPFTYELPKDLSSHNARKYLEF
                     ISQKLDGDKLTKEDSL"
     gene            4336..4800
                     /locus_tag="P6L01_001677"
     CDS             4336..4800
                     /locus_tag="P6L01_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030745.1"
                     /translation="MKRLLGLLLVSTLVLSACGNDENQEESKKEVKSKEKKIEKEKEN
                     KSKKDKEKEVATQQQPDNQTVEQPQSQEQSVQQPQQQIPQNSVPQQNVQVQQNKKQKV
                     DLNNMPPTDFSTEGMSEQAQKQIEELSMQKDYHGLSQREYNDRVSEIINNDN"
     gene            4859..5896
                     /locus_tag="P6L01_001678"
     CDS             4859..5896
                     /locus_tag="P6L01_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDP3030746.1"
                     /translation="MKTRCYDGKKWQYEFKHEGKRYRKKGFRTKREANSAGLDKLNEL
                     RSGFNIDNYITLEEYFENWIKTYKQPVVKENTYRHYRNALQHIQKHKIGKMELSKINR
                     QVYQKFINDYSKEHAKETIRKTNGAIRSALDDALYDGLIFKNPAYKVNYKAGKPTKSE
                     QEKFISVTEYEILKDHVRKKRTRSSLALFIMICTGCRVSGARNIKIEHINQVKNTIFI
                     DERKTNTSPRYISIAKSDMKHIMDVISTFAISYDGYIFKEGGSIINLHAINNALKSAC
                     RVNNIPIITSHALRHTHCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEE
                     DKKTTKILESM"
     gene            5953..6777
                     /gene="sph"
                     /locus_tag="P6L01_001679"
                     /pseudo
     CDS             5953..6777
                     /gene="sph"
                     /locus_tag="P6L01_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            complement(7015..8031)
                     /gene="lukG"
                     /locus_tag="P6L01_001680"
     CDS             complement(7015..8031)
                     /gene="lukG"
                     /locus_tag="P6L01_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit G"
                     /protein_id="HDP3030747.1"
                     /translation="MIKQLYKNITICSLAISTALTVFPATSYAKINSEIKAVSEKNLD
                     GDTKMYTRTATTSDSQKNITQSLQFNFLTEPNYDKETVFIKAKGTIGSGLRILDPNGY
                     WNSTLRWPGSYSVSIQNVDDNNNTNVTDFAPKNQDESREVKYTYGYKTGGDFSINRGG
                     LTGNITKESNYSETISYQQPSYRTLLDQSTSHKGVGWKVEAHLINNMGHDHTRQLTND
                     SDNRTKSEIFSLTRNGNLWAKDNFTPKDKMPVTVSEGFNPEFLAVMSHDKKDKGKSQF
                     VVHYKRSMDEFKIDWNRHGFWGYWSGENHVDKKEEKLSALYEVDWKTHDVKFVKVLND
                     NEKK"
     gene            complement(8053..9108)
                     /gene="lukH"
                     /locus_tag="P6L01_001681"
     CDS             complement(8053..9108)
                     /gene="lukH"
                     /locus_tag="P6L01_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit H"
                     /protein_id="HDP3030748.1"
                     /translation="MKNKKRVLIASSLSCAILLLSAATTQANSAHKDSQDQNKKEHVD
                     KSQQKDKRNVTNKDKNSTVPDDIGKNGKITKRTETVYDEKTNILQNLQFDFIDDPTYD
                     KNVLLVKKQGSIHSNLKFESHKEEKNSNWLKYPSEYHVDFQVKRNRKTEILDQLPKNK
                     ISTAKVDSTFSYSSGGKFDSTKGIGRTSSNSYSKTISYNQQNYDTIASGKNNNWHVHW
                     SVIANDLKYGGEVKNRNDELLFYRNTRIATVENPELSFASKYRYPALVRSGFNPEFLT
                     YLSNEKSNEKTQFEVTYTRNQDILKNRPGIHYAPPILEKNKDGQRLIVTYEVDWKNKT
                     VKVVDKYSDDNKPYKEG"
BASE COUNT         3317 a         1485 c         1422 g         3246 t
ORIGIN      
        1 ttaccaattg ataatagtcg tctattattt gcttcgtcat agtagtaata gatgactttt
       61 ttgttttgag cttccatttg ctgtgccctc ctgttaagtt gtttgttttt ctcctaaaaa
      121 cttattaaca aagtattgtt gtcctttgcc tgttactttt ggcgtcttac taattgatgt
      181 gtgaccgtct gaatgtgtga ttgatgtttc tttaatttcg aataactcac gttccattga
      241 atactgtgta ggcatgttat aatccacacc cttgcgttta ataaggaatc cgttttgacg
      301 taaccactca aacaatctgc gttgcccgat gtttataccg ttttgtttaa tgatctttgc
      361 taactctcca actaaaattg atgtcttagt agtagctact gcatctgcaa atacaatttt
      421 tggtttatca cgttcaatct ttgtttctaa ttgattgatt gtgttgttag caatttttaa
      481 agcacgttgc ataatcattt ctgggctatt ccatgctttt tcaacttgga tgaagtattg
      541 tcttgcacgt ttgccaggtt cactacgttg aatcattgca atctcttttg cagtgtctag
      601 tgtgagtgcg tggtcagttt gattctgacg acctcctagt gggttatgga caaaaatgtc
      661 cgtgactata taatcgatat tttcttcaaa tccgtaatca ctcattcttt caaaccattt
      721 tttgtatgga gtcttaacct ctaatgcttg atgaagttct cgaccgctga ttgcgatttc
      781 tccattttct ttttcttgta tgttgaacat ttcgcctatg ttcgattttg tttgtaatgc
      841 ttgcatattg tttgtgctcc tttctgctat actcctatta aggaggtgaa tgacttatga
      901 ctgatgaagc taaatttgtc cttttacaac tttattcaat ttaccttgat agaattgacg
      961 aaggtatgtc taaacgttct gcatcttatt tcggtagtga tgaatcctca tttaacgctt
     1021 tctttttagg ttttaatttt gaagactata tcgatgcagt tcttgaatta aagcatagag
     1081 attttgtaat tgcttctgct gaagatggcg gttttcttga gatggctctt tctcgagaag
     1141 gtatcgccta ctcagaatca gaatccaaaa aagattacaa aacacttatg ggtttaatta
     1201 gagatttgaa aaaattaata atctaaaatc caatcatctg ctattaaatc gtctgcgcta
     1261 ggctgccacc ttccggcggc ggtttgtctt tttttcttat agtgtctaga tacgactagg
     1321 cattgataac gctgcaaatt agttggtaat atcccataca catcttgatt ctctctcctt
     1381 atacttattc ctttctccat cgctagcttc gttgcttctt gaatgttcat ttgttattcc
     1441 tcctattaag atgtttgttt ttctttaaat gctaaaataa ttgatttctt tttatcattc
     1501 gtaaatacga aattttcgta ttaattacct aaaaaaatat catcatattt aacattaaaa
     1561 gcactcatat acttagaaag taaactatct ttaatgtttg tagagtcttt ttccatattt
     1621 tgaattgtac gtgatgagac cttaaataaa tctcctaact ctttttgagt caatccgtaa
     1681 tcagtcctca actcttttaa tgttttcatg ttgtcaccgc ctttcgtaaa cctaatataa
     1741 tacgaaattt tcgtattgtc aacattaaat acgttttttt cgtaaaaaac tttactatga
     1801 tatgaaaatt tcgtataata agaaaaaagg aggtaagtaa tatgaacaaa gaaagaaata
     1861 ttattatagc caaaaacatt agaaaatttc tcaacgattc aaatatgtct caaaagaaac
     1921 ttgctgaact cattaacata aaaccatcta ctttaagcga ttatttaaat ttacgttcca
     1981 acccctctca cggcgttata caaaggatag ctgatgtttt cgaggttggt aaaagcgaca
     2041 tagatactac atacaaagac gataacgaca tcacttccat atacaacaaa ctcacacctc
     2101 cccgccaaga aaacgtactt aactatgcaa atgaacaatt ggaagaacag aattctaaag
     2161 gagataacgt tgtagatatt aattcatata aacaggagaa aactccagtt aacgtcaatg
     2221 gttgcgtctc tgctggtgta ggagaacgtt tacacgatga aacgctattt actgaaatgg
     2281 ttaaaggacc tatccccaca cacgatttag cgttaaaagt aaatggtgat tctatggaac
     2341 ctatgtttaa agatggcgaa atcatatttg tggagaaaac tcacaatata aagaatggac
     2401 aaattggtat attcatcatt gaagaagaag cgtacgttaa gaaagtcttt gttgaagatg
     2461 atagattgac tctagtttca ctaaataaag attacgacga tctacacttt tatagaaatg
     2521 aaagtgtgag gttaattgga aaagttattt tataaaagga gcacttgcaa atgaaaaaat
     2581 atgatattgc agtcttagac tttgaaacta tgaatgaaca tatgaacagc ccttgcgaag
     2641 ttgctgtatc tttaattaag gatttatcaa tagtaaaagt ttattcatct tatattaatc
     2701 ctcctaataa tagatataac ttgaaaaacg ctaaaataca taaaatacct gaagatgtca
     2761 tattaaaagc acctaaatat ccagatattt accaagaaat tctctatctt ttaaaagaat
     2821 cacatttaat tattgctcat aatgcacttt ttgatatttc agtattaaaa aatactaata
     2881 attattatga cttacctgtt ccaaacttca tgtatgtcga tagtataaat atctttagaa
     2941 gcttccacgc aatctctagt tttaaattag aaaatttgtg tagcttatat gatatcgata
     3001 aagaaaaatt acattctgct aaatttgacg tgctagcttt atcgaagatg ttgatatcac
     3061 tcgctaaaaa caatcagcat tatagtgtat taaaattaat acattatatg cctaagcaat
     3121 acattagatt tagcaaatat tctaactctc caactaaact tttcgattca ggatttcaaa
     3181 aaattcatat gaaaatatct gagattaata aaatagaagt ggaaagtgta atccctattt
     3241 taaaagataa aaatgttgtt tttacaggta attttgacac tgaaaaacaa gatttaatga
     3301 tattaactag aaagaaagga gcttatatca gaagtgacgt aactgcaaaa acagatattt
     3361 tagtcgaagg tgttcaagat gataaatata aagatgtgaa cggactagtt tcaaaacaac
     3421 gaaaagctcg agaatatgtt ggaaatggtg caaaaattca atttttaaat gaagaagact
     3481 taataaattt aataaaggaa taataacgat gatcaaaaaa atttttacaa aaaagcatgt
     3541 attcttagtt atagaagatg aaaaccataa tcacagtgat gctgtttttg gaaaaagtat
     3601 attactttca atttacgtcg gtgtgaataa aaagactaat tctaaatcag ggaaatttat
     3661 ataccttgac agatctaaaa gaatcgttag acaatctgat atcaccaaaa tagaatcagc
     3721 taacgaaaat gatgtagatt tttataattt actgaagaaa gaaaaggaaa ttgtttattc
     3781 caaaaatata gtagataaat acaatttagc gaactatata atttactacg aagttagtac
     3841 taaagaataa accaatccat tatttcataa tactaacctt aaatttacag aggttttaat
     3901 tatgaaacat gaaaaaagca atcttaactt taagtcttat atttattacc tactacctca
     3961 cttttaaata tatgtggatt aaagaattga agtattaatt atgcttattt aaaaaagacg
     4021 tctatttcag cagtgtttga aaggaagttt ataatgaaaa taactaattg caaaataaaa
     4081 agagaaactg taatatacga agttttaact agtggtaatc aaccattcac ttatgagtta
     4141 cctaaagatt tatcgtcaca taatgcgcgt aaatacttgg aatttatttc acaaaaatta
     4201 gatggcgata agttaaccaa agaagattca ttatgatttt actaaacaaa aaaacgccta
     4261 ctagtgtgaa aacgtattga ttaatagcgc ctatatggag ttttaatata aaaagtaagc
     4321 aaaggagaaa tgagaatgaa aagattgtta ggtttattat tagtgagcac gttagtgtta
     4381 agtgcatgtg gaaatgatga gaatcaggaa gaatctaaaa aagaagttaa atcaaaagaa
     4441 aagaaaattg agaaggaaaa ggaaaataaa tcgaaaaaag ataaggaaaa agaagttgca
     4501 acacaacaac aaccagacaa tcaaaccgtt gaacaacccc aatcacaaga gcaatcggtt
     4561 caacaaccgc aacaacagat accacaaaat agtgttcctc agcaaaatgt ccaagttcaa
     4621 caaaacaaaa agcaaaaagt tgatttaaat aatatgcctc ccactgattt ttctacagag
     4681 ggtatgtctg agcaggctca aaaacaaatt gaagagcttt caatgcaaaa agactatcat
     4741 ggtctgtcac aaagagaata caatgataga gtttctgaaa ttataaataa tgataattga
     4801 ttccacaggg tagcccgcct acccttatta ttttttgcca attttgagga gggaacgcat
     4861 gaaaacacgt tgttacgatg gtaaaaaatg gcaatatgaa tttaagcatg aaggaaaaag
     4921 ataccgtaag aaaggtttta gaacaaagcg tgaagctaat tctgctggac tagacaagtt
     4981 aaatgagtta agaagtggtt ttaatataga taactatata actcttgaag aatacttcga
     5041 aaattggatt aaaacatata aacaacctgt tgttaaagaa aatacctacc gtcattatag
     5101 aaatgcatta caacatatac aaaaacataa aataggtaaa atggagttat caaagataaa
     5161 tagacaagtt tatcagaaat tcataaacga ctattcaaaa gaacacgcaa aagaaactat
     5221 aagaaaaaca aacggtgcta ttcggtcagc tttagatgac gcattatatg atggacttat
     5281 ttttaaaaac cccgcttata aagttaatta taaagccgga aaacctacga agtcagaaca
     5341 agaaaaattc atctcggtaa ctgaatatga aatactaaaa gatcacgtca gaaagaagag
     5401 aactcgttca tcattagcgc tattcataat gatttgtacg ggttgtcgtg tcagtggtgc
     5461 aagaaatata aagattgagc atatcaacca agtgaaaaac actatattta ttgacgagcg
     5521 aaaaaccaat acttccccta gatatatcag tatcgctaaa tctgatatga aacacattat
     5581 ggacgtcata agtacatttg caattagcta tgatggttac attttcaaag aaggcggatc
     5641 tataattaac cttcatgcta tcaataatgc tttgaaatca gcctgtagag tcaataatat
     5701 accaattatt acatcgcacg cattaagaca cactcattgt tcttatttac tagcaaaagg
     5761 tgtatctata cattacattt ctaaaagatt aggtcataaa aatatagcaa taactacatc
     5821 tgtgtattct catttgttag aagaaaaatt taatgaagag gacaaaaaaa caactaaaat
     5881 tttagaaagt atgtaattta gggacccatt agggaatcca aacccaataa atactgttgt
     5941 tacaaggttt ctatgtatcc aaactgggga caatataaac gcgctgattt aatcggacaa
     6001 tcttcttata ttaaaaataa tgatgtcgta atattcaatg aagcatttga taatggtgca
     6061 tcagacaaat tattaagtaa tgtgaaaaaa gaatatcctt accaaacacc tgtactcggt
     6121 cattctcaat caggttggga caaaactgaa ggtagctact catcaactgt tgctgaagat
     6181 ggtggcgtag cgattgtaag taaatatcct attaaagaaa aaatccagca tgttttcaaa
     6241 agcggttgtg gattcgataa tgatagcaac aaaggctttg tttatacaaa aatagagaaa
     6301 aatggtaaga acgttcacgt tatcggtaca catacacaat ctgaagattc acgttgtggt
     6361 gctggacatg atcgaaaaat tagagctgaa caaatgaaag aaatcagtga ctttgttaaa
     6421 aagaaaaata tccctaaaga tgaaacggta tatataggtg gcgaccttaa tgttaataaa
     6481 ggcactccag agttcaaaga tatgcttaaa aacttgaatg taaatgatgt tctatatgca
     6541 ggtcataata gcacatggga ccctcaatca aattcaattg cgaaatataa ttaccctaat
     6601 ggtaaaccag aacatttaga ctatatattt acagataaag atcataaaca accaaaacaa
     6661 ttagtcaatg aagttgtgac tgaaaaacct aagccatggg atgtatatgc gttcccatat
     6721 tactacgttt acaatgattt ttcagatcat tacccaatca aagcctatag taaatagtgc
     6781 tcaactaact aataactcgc ttcgttctaa aaggacgaag cgagttatat tgttaaaatt
     6841 tgaattgact tacattttaa taaaatcatc ttaacaactt taatttttca ttaatacaag
     6901 tctttactct acactcaaac aagattcata cactgcacat cataataaat ctatctactc
     6961 aaatataaat aaaagttacc tactacattc tatgtagcag gcaactttta ttacttattt
     7021 cttttcatta tcattaagta cttttacaaa cttcacatca tgtgtcttcc aatcaacttc
     7081 atataatgct gataattttt cttctttttt atctacatgg ttttcaccag accaatagcc
     7141 ccagaaacca tggcgattcc aatctatttt aaactcatcc attgatcttt tataatgaac
     7201 aacaaattgt gatttacctt tgtctttttt atcatgtgac ataacagcta aaaattctgg
     7261 attaaaccct tcagacacag ttacaggcat tttgtcttta ggtgtgaaat tatctttcgc
     7321 ccataaattt ccatttcgtg ttaaagaaaa gatttcactt ttagttctat tatcactatc
     7381 attagttaat tgtctcgtat ggtcatgtcc catattattt atcaaatgtg cttctacttt
     7441 ccaacctaca cctttatgtg acgtagattg atcaagtaat gtacgatatg atggttgttg
     7501 ataactaatc gtctctgaat aattactctc ttttgtaata tttccagtta agcctccacg
     7561 attaatcgaa aaatctccac ctgttttata accatacgta tatttaactt ctcttgattc
     7621 atcctgattt tttggtgcaa agtcagtcac atttgtattg ttgttgtcat caacattttg
     7681 aattgaaact gaataagatc caggccatct taatgtacta ttccaataac catttgggtc
     7741 taaaattctc aaaccactac caattgtacc ttttgcttta ataaatactg tttctttatc
     7801 ataattaggt tcagttaaga aattaaattg taagctttga gtaatatttt tttgactatc
     7861 acttgttgta gctgtacgtg tatacatttt agtatcacca tcaagattct tctcagaaac
     7921 agctttaatt tcagaattaa tttttgcata agaagttgcc ggaaatacag ttaatgcagt
     7981 agatattgct aaactacaaa ttgtgatgtt tttgtataat tgtttaatca ttagtaatcc
     8041 gccctttcaa tattatcctt ctttataagg tttattgtca tcagaatatt tatcaacgac
     8101 tttaactgtt ttatttttcc aatcaacttc ataagtgaca attaaccttt gaccatcttt
     8161 atttttttct aaaattggag gtgcataatg tattccaggt ctgtttttca aaatatcttg
     8221 atttcgtgtg tatgttactt caaattgcgt tttctcattt gacttttcat tagataaata
     8281 agttaaaaat tctggattaa agccacttct tactaatgct gggtatctat attttgaagc
     8341 aaagcttagt tcagggtttt ctacagtagc aattctcgta tttctataga ataataattc
     8401 atcatttcta tttttcactt ctccaccata cttcaagtca ttcgcaataa ctgaccagtg
     8461 tacatgccag ttattatttt taccgctggc aattgtgtca taattttgct gattataact
     8521 aatcgttttg gagtagctat ttgatgaagt tcgtccaata cctttcgttg aatcgaattt
     8581 accacctgag ctatatgaaa atgtactgtc tactttcgca gttgaaattt tatttttcgg
     8641 caattggtct aatatttcag ttttacgatt tctttttact tgaaaatcta catggtactc
     8701 acttggatac tttaaccaat ttgaattttt ttcttcttta tgagattcaa actttaaatt
     8761 tgaatgaatt gagccttgtt ttttaacaag taatacattc ttgtcataag ttggatcatc
     8821 gataaagtcg aattgtaaat tttggagtat atttgttttc tcatcatata ctgtttcagt
     8881 tcgttttgtg attttaccgt ttttcccaat atcatcaggt actgttgaat ttttatcttt
     8941 attagtaaca ttacgtttgt ctttttgttg agacttatca acatgttctt tcttattttg
     9001 atcttgcgag tctttatgag ctgaatttgc ttgagtcgtt gctgctgata acaataaaat
     9061 tgcacatgat aatgatgacg ctattaaaac acgtttttta tttttcattg ttataacctt
     9121 ctttcgtatg attgatattt gttgatatgt atcgacatgt gaataatatc acaaaaatag
     9181 agaatatata tttaactatt tattaaatga ttttgttaat attattaaat actttatcct
     9241 ctttaaaaat aatgtgtgta caaagtcatt aatttagcaa atatttttat ttagtagtta
     9301 ataaccatcg atttgaaatt tatatataat tattagctaa ataatatcct gcatctttct
     9361 catacaattt actataaaat taagcatacc cgatatcagt gttaataaga tcgttgatac
     9421 tagacagtta atttcataga acgaaatcaa ataacacgct actttctgca
//
LOCUS       DAOBBH010000098         9399 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.098, whole genome shotgun sequence.
ACCESSION   DAOBBH010000098 DAOBBH010000000
VERSION     DAOBBH010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9399
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.098"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            80..355
                     /gene="rpsP"
                     /locus_tag="P6L01_001682"
     CDS             80..355
                     /gene="rpsP"
                     /locus_tag="P6L01_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDP3030749.1"
                     /translation="MAVKIRLTRLGSKRNPFYRIVVADARSPRDGRIIEQIGTYNPTS
                     ANAPEIKVDEALALKWLNDGAKPTDTVHNILSKEGIMKKFDEQKKAK"
     gene            543..1046
                     /gene="rimM"
                     /locus_tag="P6L01_001683"
     CDS             543..1046
                     /gene="rimM"
                     /locus_tag="P6L01_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDP3030750.1"
                     /translation="MRVEVGQIVNTHGIKGEIKVKSNSDFTDVRFQPGQVLTVVHNNN
                     DLEYTVKSHRVHKGLHMLTFEGINNINDIEHLKGSSIYQERDHEDIVLEENEFYYSDI
                     IGCTVFDDQETPIGRVINIFETGANDVWVIKGSKEYLIPYIADVVKEVDVENKKIIIT
                     PMEGLLD"
     gene            1046..1783
                     /gene="trmD"
                     /locus_tag="P6L01_001684"
     CDS             1046..1783
                     /gene="trmD"
                     /locus_tag="P6L01_001684"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDP3030751.1"
                     /translation="MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAIN
                     KHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSK
                     ADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
                     EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKR
                     PDLIEKYPLTNEDKQILERYKIGLKKG"
     gene            1886..2236
                     /gene="rplS"
                     /locus_tag="P6L01_001685"
     CDS             1886..2236
                     /gene="rplS"
                     /locus_tag="P6L01_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDP3030752.1"
                     /translation="MTNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQV
                     FEGIVIKRRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYYL
                     RSLRGKAARIQEIR"
     gene            complement(2480..5086)
                     /gene="yfhO"
                     /locus_tag="P6L01_001686"
     CDS             complement(2480..5086)
                     /gene="yfhO"
                     /locus_tag="P6L01_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosyltransferase
                     YfhO"
                     /protein_id="HDP3030753.1"
                     /translation="MLKKWLNSNVKQFFVITFISVILTLILFSTHIYDYIVNGTVFSG
                     AGDGFRQMMPFQMYLYEHLSSFSSLYDASFGLGGDYMKGLSYYYSLSPLMWLNFLFIK
                     IGETVGIFNPTTIHFWPTNQLIMAMIRAIITFVVTFYLFKILHFKRSTNMIATILYGM
                     STVVIYFNFTWSFYGNLLYLLPLSILGLERYFQQRKIGIFIVAIALTLFSNFYFSYYQ
                     AIIIGCYYLYRLIFTYKYDIVSRTQKFICVISATVLSVLSSVFGLFTGISAFLENDRK
                     QNPNVDIPFLTPLDYHYFFFSDGFYITISILTIVALLSFKLYRFYFYRLFAIVTWILF
                     IGSLSQYFDSAFNGFSFPERRWVYILALSSSALCGLFIQHLSTLNMKYYLIRTIPVSI
                     IALLYVLLSPTHPLALIVGIILLMVLAVILKFSLWRYKKLTVAILVLIVMIQQIVILD
                     NNKNMAIKPYQQSLSTLKQHDYHSNYVNQLIKKINQNATGPFNRIDYMSDYALNSPFI
                     YHYNGISLYSSIFNGDILKYYDKTLQINMPIDKNSTYRLLGNRQNLLSLWNVNDRIRV
                     NHDDNLPYRFKINSEHKDNKVRWIHSKNTIHYPSAHITNKVFSNKELKSPLDKEQAML
                     QGIVSNNTKDVNTHFKANKNLLSDSTIKLNSSAWQSPTKHLLQVKQNNGGLTVQLPKS
                     VSNQFKDLYFEMDLELLSPDKAHDVKVNEYTQERNKLTYKYRRFVTPVTIRIKASDRI
                     RLSLPKGKYRVNLKGIYGEDYTTLKDASNSLEAVKVSKTKQGYTITKNKNSSGYIVLP
                     TAYNQGMKATSGDQSLKVAQVNGVMTGIKAPKNITKIQLSYTPPYYYLLITITIFGII
                     CSIIFTRWARQK"
     gene            5487..6371
                     /gene="ylqF"
                     /locus_tag="P6L01_001687"
     CDS             5487..6371
                     /gene="ylqF"
                     /locus_tag="P6L01_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDP3030754.1"
                     /translation="MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMI
                     DEVINQKPRVVILNKKDMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIK
                     ATAEKFEREKAKGLKPRAIRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQW
                     IKVGNALQLLDTPGILWPKFEDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQNDL
                     ARLKSHYNIEVPEDAEIIAWFDAIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNY
                     CFDIFKDMTEELANDANN"
     gene            6355..7122
                     /locus_tag="P6L01_001688"
     CDS             6355..7122
                     /locus_tag="P6L01_001688"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDP3030755.1"
                     /translation="MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKA
                     LEKEQALKEKYVEMTYFENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNY
                     LGLDDSKKVPITKRLELNEALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGL
                     SVQPTHLLIDAMTLDNALPQVSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEY
                     GFEKNAGYGTKQHLLAIDDIGIMKEHRKSFEPIKSLL"
     gene            7231..8397
                     /gene="sucC"
                     /locus_tag="P6L01_001689"
     CDS             7231..8397
                     /gene="sucC"
                     /locus_tag="P6L01_001689"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDP3030756.1"
                     /translation="MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEAVEKAKELNSDVYV
                     VKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGC
                     AIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQA
                     RRIAFNINIPKESVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINF
                     DDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTI
                     NHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVE
                     AVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA"
     gene            8419..9327
                     /gene="sucD"
                     /locus_tag="P6L01_001690"
     CDS             8419..9327
                     /gene="sucD"
                     /locus_tag="P6L01_001690"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDP3030757.1"
                     /translation="MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKG
                     GQVVEGVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPV
                     LDMVKVKRYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYE
                     AVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEA
                     AEWIKANMTKPVVGFIGGQTAPPGKRMGHAGAIISGGKGTAEEKIKTLNSCGVKTAAT
                     PSEIGSTLIEAAKEAGIYESLLTVNK"
BASE COUNT         3400 a         1307 c         1785 g         2907 t
ORIGIN      
        1 tacaaacatt catacacctg ttaatattat ttcttgtaga aaataaaaat taaaacatga
       61 cttaaaggag attttataaa tggcagttaa aattcgttta acacgtttag gttcaaaaag
      121 aaatccattc tatcgtatcg tagtagcaga tgctcgttct ccacgtgacg gacgtatcat
      181 cgaacaaatc ggtacttata acccaacgag cgctaatgct ccagaaatta aagttgacga
      241 agcgttagct ttaaaatggt taaatgatgg tgcgaaacca actgatacag ttcacaatat
      301 cttatcaaaa gaaggtatta tgaaaaaatt tgacgaacaa aagaaagcta agtaatttag
      361 cgtaaaattg ttctaacaat aagaataact cgtttacact gacagttatt actcaatgat
      421 acgttgggaa tatcacatgt tagtaatata gaacgtattg ggtaccataa tggtgccctt
      481 tttctttgaa ttattttcaa ttaaaataga agtgttcaaa gcatagagtt ggaggtaata
      541 gaatgagagt tgaagttggt caaattgtta acacacatgg tattaaaggt gaaattaaag
      601 taaaatccaa ttcagacttt acagacgttc gttttcaacc cggtcaagtg ctgacagttg
      661 tgcataacaa taacgacctt gaatatactg ttaagtcaca tagagtgcat aaagggcttc
      721 atatgcttac atttgaaggt attaataata ttaatgatat tgagcattta aaagggagtt
      781 ctatttatca agagcgtgat catgaagata tcgtacttga ggaaaatgaa ttttattatt
      841 cagatattat aggatgtaca gtttttgatg atcaagaaac accaataggt cgtgtaatta
      901 atatatttga aacaggtgcg aatgatgtgt gggtgattaa aggatctaaa gaatatttga
      961 ttccttatat tgctgatgtt gtaaaagaag tggatgttga aaataaaaaa attatcatca
     1021 cgccaatgga aggattgttg gattaatgaa aattgattat ttaactttat ttcctgaaat
     1081 gtttgatggt gttttaaatc attcaattat gaaacgtgcc caagaaaaca ataaattaca
     1141 aatcaatacg gttaatttta gagattatgc aattaacaag cacaaccaag tagatgatta
     1201 tccgtatggt ggcggacaag gtatggtgtt aaagcctgaa cctgttttta atgcgatgga
     1261 agacttagat gtcacagaac aaacacgcgt tattttaatg tgtccacaag gcgagccatt
     1321 ttcacatcag aaagctgttg aattaagcaa ggctgaccac atcgttttca tatgcggaca
     1381 ttatgaaggt tacgatgaac gtatccgaac acatcttgtc acagatgaaa tatcaatggg
     1441 tgactatgtg ttaactggcg gtgagttacc ggcaatgaca atgactgatg ctattgttag
     1501 actgattcca ggtgttttag gtaatgaaca gtcacatcaa gacgattcat tttcagatgg
     1561 gttattagag tttccgcaat atacacgtcc gcgtgaattt aagggtctaa cagttccaga
     1621 tgttttattg tctggaaatc atgccaatat tgatgcatgg agacatgagc aaaagttgat
     1681 ccgcacatat aataaaagac ctgacttaat tgaaaaatat ccattaacta atgaagataa
     1741 gcaaatatta gaaagatata aaataggatt gaaaaaaggt tagtattgtg ctaacatatt
     1801 ctgagtgtgg aaacacaaaa atcactatga tccgctgcta tatatttgtc gaggcaagaa
     1861 cataggttga agaggagaat ttataatgac aaatcacaaa ttaatcgaag cagtaactaa
     1921 atcacaattg cgtacagact taccaagttt ccgtcctggt gatactttac gtgtacacgt
     1981 acgtatcatt gagggtactc gtgagcgtat ccaagtattc gaaggcattg taattaaacg
     2041 tcgtggcggt ggcgtttctg aaacgtttac agttcgtaaa atttcatcag gtgttggcgt
     2101 ggaacgtaca ttcccattac acacaccaaa aattgaaaaa atcgaagtta aacgtcgtgg
     2161 taaagtacgt cgtgctaaat tatattactt acgtagttta cgtggtaaag ctgctagaat
     2221 ccaagaaatt cgttaatcag catttaaaca aagctatgta tgagtcaaat tcgactcaaa
     2281 caataaagcc atctaggtca cttttatgga gtgatataga tggctttttt tgctgtttag
     2341 ttaatgtaaa gttgataatg taattgtttt ttcttattta ttcataacgt gtttgctcaa
     2401 aaatgcttat ataaacaaaa atctactaag tgccattgaa aaggacttag tagatttatt
     2461 aaatatatgc gtagcaacat tatttttgtc ttgcccatct cgtgaaaata atactacata
     2521 taatgccaaa tatagtaatt gttataagta aataatagta tggtggggta tagctcaatt
     2581 gaatctttgt tatattttta ggtgctttaa tgccggtcat aacaccattt acttgtgcaa
     2641 ctttaagact ttgatcacct gatgtcgctt tcataccttg attatatgct gttggcaaaa
     2701 caatataccc agatgaattt ttatttttag taatagtata accttgcttt gtcttactaa
     2761 ctttgacagc ctctaatgaa tttgaagcgt ctttaagcgt ggtataatct tcgccgtata
     2821 tcccttttaa atttactcga tacttacctt taggcaatga taatctaatt ctatctgaag
     2881 ctttaatgcg tatcgttacg ggtgttacaa agcgtcgata tttataagtg agtttatttc
     2941 tttcttgtgt atattcattc actttaacat catgagcttt atccggcgaa agtaattcta
     3001 aatccatttc aaaatacaaa tctttaaatt gattagaaac tgattttggc aactgtacag
     3061 ttagaccacc attattttgt ttaacttgta ataaatgttt tgtaggagat tgccaggctg
     3121 aactatttaa tttaattgtt gaatctgata gtaaattttt attggcttta aaatgtgtat
     3181 taacatcttt agtattgtta gaaacaatcc cttgcaacat tgcttgttct ttatctaatg
     3241 gagattttaa ttctttattg gaaaagacct tatttgtaat atgtgcactt ggataatgga
     3301 tggtattttt agaatgaatc caacgaactt tattgtcttt gtgttcagag ttaattttaa
     3361 atctatatgg taagttgtca tcatgattca ctctaattcg atcattaaca ttccaaagtg
     3421 atagtaaatt ttgacgattg ccaagtaatc tataagtgct gtttttatca attggcatat
     3481 taatttggag tgtcttgtca taatatttta aaatgtctcc attaaaaata ctagaatata
     3541 atgaaatgcc attataatga tatataaatg gtgaatttaa tgcatagtct gacatataat
     3601 caatgcgatt aaatgggcct gttgcatttt gatttatctt ttttataagc tggtttacat
     3661 agttactatg gtaatcatgt tgtttcaacg ttgataatga ttgttgataa ggtttgattg
     3721 ccatattttt gttgttatct aaaatgacga tttgttgaat cataacgatt aatactaata
     3781 ttgcaacggt taatttttta taacgccata aactaaattt taaaataacg gcaagcacca
     3841 ttagcaggat aatacctact ataagtgcaa gtgggtgtgt cggtgatagt aatacataaa
     3901 gtaatgcgat gatacttact ggtattgttc tgattaaata atatttcata tttaatgttg
     3961 ataaatgttg aataaacaat ccgcaaagag cgcttgatga tagtgctaag atatacaccc
     4021 aacgcctttc tggaaatgaa aaaccattaa aagcactgtc gaaatactgt gataatgaac
     4081 caataaataa tatccatgtt actattgcga aaagtctata aaagtaaaaa cgatacagtt
     4141 tgaatgacaa taatgcaacg atagtaagaa ttgaaatcgt aatataaaat ccatcgctaa
     4201 agaaaaaata atgataatca agtggtgtca aaaacggtat atcaacattg ggattttgct
     4261 ttctgtcatt ttccaaaaac gcagaaatgc cagtgaataa accaaataca cttgataaca
     4321 cactcaaaac tgtagcagat atgacgcaaa taaatttttg tgttctagag acaatgtcat
     4381 atttgtaagt gaaaatgagt cgatataaat agtagcaacc tataataata gcttgataat
     4441 aactgaaata aaaattgcta aatagtgtta aggctatcgc aacaatgaaa ataccgattt
     4501 tgcgttgttg aaaatatctt tccaaaccaa gaatcgataa tggcaataaa tataataaat
     4561 ttccataaaa tgaccaagta aaattaaagt atataacgac agttgacatg ccgtataaaa
     4621 tcgtagcgat catatttgtt gagcgtttaa agtgtaatat tttaaataag tagaaggtca
     4681 cgacaaatgt tatgatagct cgtatcatag ccataataag ttggtttgtc ggccaaaaat
     4741 gtattgttgt cggattaaat ataccaaccg tttctcctat tttaatgaat agaaaattta
     4801 gccacattaa aggtgacagc gaataataat atgatagtcc tttcatataa tcgccaccta
     4861 atccaaacga tgcatcatat aaactagaaa aactacttag atgttcatac aaatacattt
     4921 gaaatggcat catttgacgg aatccatctc cagccccact aaaaacagta ccattcacaa
     4981 tataatcata gatatgagta gaaaataaaa taagcgttaa tattacacta atgaaagtta
     5041 taacaaagaa ttgtttgacg tttgaattta gccacttttt taacacaaca ttatcctcaa
     5101 ctttcaaatt taaaattaag tttaactgaa actaaagtta atgaggttct tgataggtaa
     5161 agacgaagat gactgtggaa cagatacctt atcatagtta cttaaacttt ggatcatttt
     5221 cagtttatca ttaaacaaat atattgaata ataaaaatgt catactgata aagatgaatg
     5281 tcacttaata agtaacttag atttaacaaa tgatgatttt taattgtaga aaacttgaaa
     5341 taatcacttt tacctaaatc taaagcattg ttaagaagtg tgacaatgtt aaaataaata
     5401 tagttgaatt aatgaatttg ttctataatt aacaggttat agattttaat aatgagaaaa
     5461 gaattgacga aagtaaggtg aattgaatgg ttattcaatg gtatccagga catatggcga
     5521 aagccaaaag agaagtaagt gaacaattaa aaaaagtaga tgtagtgttt gaactagtag
     5581 atgcaagaat tccatatagt tcaagaaacc ctatgataga tgaagttatt aaccaaaaac
     5641 cacgtgttgt tatattaaat aaaaaagata tgtctaattt aaatgagatg tcaaaatggg
     5701 aacaattttt tattgataaa ggatactatc ctgtatcagt ggatgctaag cacggtaaaa
     5761 atttaaagaa agtggaagct gcagcaatta aggcgactgc tgaaaaattt gaacgcgaaa
     5821 aagcgaaagg acttaaacct agagcgataa gagcaatgat cgttggaatt ccaaatgttg
     5881 gtaaatccac attaataaat aaactggcaa agcgtagtat tgcgcagact ggtaataaac
     5941 caggtgtgac caaacaacaa caatggatta aagttggtaa tgcattacaa ctattagaca
     6001 caccagggat actttggcct aaatttgaag atgaagaagt cggtaagaag ttgagtttaa
     6061 ctggtgcgat aaaagatagt attgtgcact tagatgaagt tgccatctat ggattaaact
     6121 ttttaattca aaatgattta gcgcgattaa agtcacatta taatattgaa gttcctgaag
     6181 atgcagaaat catagcgtgg tttgatgcga tagggaaaaa acgtggctta attcgacgtg
     6241 gtaatgaaat tgattacgaa gcagtcattg aactgattat ttatgatatt cgaaatgcta
     6301 aaataggaaa ttattgtttt gatattttta aagatatgac tgaggaatta gcaaatgacg
     6361 ctaacaatta aagaagttac gcagttgatt aatgcggtta atacaataga agaattagaa
     6421 aatcatgaat gctttttaga tgagcgaaaa ggtgttcaaa atgccatagc taggcgcaga
     6481 aaagcgttag aaaaagaaca agctttaaaa gaaaagtatg ttgaaatgac ttactttgaa
     6541 aatgaaatat taaaagagca tcctaatgct attatttgtg ggattgatga agttggaaga
     6601 ggacctttag caggtccagt cgttgcatgc gcaacaattt taaattcaaa tcacaattat
     6661 ttgggccttg atgactcgaa aaaagtacct attacgaaac gtctagaatt aaatgaagca
     6721 ctaaaaaatg aagttactgc ttttgcatat ggtatcgcga cagctgaaga aatagatgaa
     6781 tttaatattt ataaagctac tcaaatcgcc atgcagcgag ctattgatgg attatcagta
     6841 caaccaacgc atttattgat agacgcgatg acgcttgata atgcactgcc tcaagtatct
     6901 ttaatcaagg gtgatgcaag aagtgtgtcc attgcagctg caagtatcat ggcgaaggtt
     6961 tttcgtgatg attatatgac acagttatct aaagattatc ctgaatacgg ttttgaaaaa
     7021 aacgcgggtt atggtaccaa acaacattta ctagcaatcg atgatattgg cattatgaaa
     7081 gagcatagaa aaagctttga acctataaaa tcgttactgt aaatcgtaaa attcaattaa
     7141 atgataaaaa agtagcattt aagtttacaa tagcgcttac attttttata attgtaaggg
     7201 aacttaacct aagtaacagg aggatggaag atgaatatcc acgagtatca aggtaaagaa
     7261 atatttcgtt caatgggcgt tgcagttcca gaaggacgag tagcatttac tgctgaagaa
     7321 gcggtggaga aagcaaaaga attaaattct gatgtatatg ttgtaaaagc acaaattcat
     7381 gctggaggta gaggtaaagc aggcggagta aaaattgcta aatctttatc tgaggtagaa
     7441 acatatgcaa aagaattatt agggaaaact ttggtgacac atcaaactgg tccagaaggt
     7501 aaagaaatta aacgtttata tatcgaagaa ggttgtgcta ttcaaaaaga atattacgtt
     7561 ggattcgtta ttgatcgtgc gactgaccaa gtaacattga tggcgtctga agaagggggc
     7621 actgagattg aagaagttgc tgcgaagact cctgaaaaga tcttcaaaga aactatcgat
     7681 ccagtaatcg gactttcacc attccaagca agacgaattg cgtttaatat taatattcct
     7741 aaagaatctg ttaacaaagc tgctaaattc ttattagcac tttataatgt attcattgaa
     7801 aaagattgtt caatcgtaga aatcaaccca ttagttacaa cagctgatgg tgatgtattg
     7861 gcattagatg ctaaaattaa ttttgatgat aatgcattat tcagacataa agatgttgta
     7921 gaattacgtg atttagaaga agaagatccg aaagagattg aagcgtctaa acatgattta
     7981 tcatacattg cattagatgg tgacatcgga tgtatggtta atggtgcagg tttagccatg
     8041 gcaacaatgg atacgattaa tcatttcggt ggaaacccag ccaatttctt agatgcaggc
     8101 ggaagcgcta ctagagaaaa agtaactgaa gcatttaaaa tcattttagg tgatgaaaat
     8161 gttaaaggta tttttgtaaa cattttcggt ggcattatga aatgtgatgt tatcgcagaa
     8221 ggtatcgttg aagctgtaaa agaagtagat ttaactttac cactagttgt acgcttagaa
     8281 ggtacaaatg ttgagttagg taaaaaaatc ttaaaagact caggattagc aattgaacca
     8341 gcagcaacaa tggctgaagg tgcacaaaaa attgttaaac tagtcaaaga agcataagga
     8401 aaggatggga gcactaagat gagtgtattt atagataaga atactaaagt aatggtacaa
     8461 ggtattacag ggtctactgc ccttttccat acaaaacaaa tgcttgatta tggtacgaaa
     8521 atagtagcag gtgtgacgcc tggtaaaggt ggtcaagttg ttgaaggcgt tcctgttttc
     8581 aacactgttg aagaagctaa aaatgaaact ggggcaacgg tttcagtcat ttacgttcca
     8641 gcaccatttg ctgcagactc aattttagaa gcagctgatg cagacttaga tatggttatt
     8701 tgtatcactg aacatattcc tgtattagac atggttaaag ttaaacgcta cttacaaggt
     8761 agaaaaacac gtttagttgg tccgaactgt ccaggtgtga ttacagcaga tgaatgtaaa
     8821 attggtatta tgcctggcta tattcacaaa aaaggtcatg ttggtgtagt atcacgttca
     8881 ggtacactaa catatgaagc agtgcaccaa ttgactgaag aaggtattgg tcaaactaca
     8941 gctgttggta ttggtggaga cccagtcaac ggaacaaact ttattgatgt tttaaaagca
     9001 ttcaatgaag atgacgaaac gaaagcagtt gttatgattg gtgaaatcgg tggtacagct
     9061 gaagaagaag cagctgaatg gattaaagcg aatatgacaa aaccagttgt aggctttatc
     9121 ggtggacaaa cagcacctcc tggaaaacgt atgggacatg ctggtgcaat catttcaggt
     9181 ggtaaaggta ctgctgaaga gaaaataaaa acattaaata gttgtggtgt gaaaacagcg
     9241 gcaacacctt cagaaattgg ttcaacatta attgaagctg ctaaagaagc aggtatttat
     9301 gaatcattat taacagttaa taaataaagt taaaagatga tataaatggc tatagcccat
     9361 tccatacttt ataaaagtat cggaaatggg ctatttgtc
//
LOCUS       DAOBBH010000099         9353 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.099, whole genome shotgun sequence.
ACCESSION   DAOBBH010000099 DAOBBH010000000
VERSION     DAOBBH010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9353
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.099"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            76..849
                     /locus_tag="P6L01_001691"
     CDS             76..849
                     /locus_tag="P6L01_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDP3030758.1"
                     /translation="MKAHKIFWLNLAAIIIISIVVSGGMFLAMKWEQIHLKDGLKKVL
                     STYPIKNLETLYEIDGHDNPHYENNDQDTWYIESSYSVVGSDELLKEDRMLLKVDKNT
                     HKITGEYDTTTNDRKDATDSTYKSYPVKVVNNKIVFTKDVKDPALKQKIENNQFLIQS
                     GDLTSILNSNDLKVTHDPTTDYYNLSGKLSNDNPNVKQLKRRYNIPSNASTKVELKGM
                     SDLKGNNHQDQKLYFYFSSPGKNQIIYKESLTYNKLSEH"
     gene            complement(1162..3168)
                     /gene="ggt"
                     /locus_tag="P6L01_001692"
     CDS             complement(1162..3168)
                     /gene="ggt"
                     /locus_tag="P6L01_001692"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDP3030759.1"
                     /translation="MVINLNDKQTKTSKEGLISVSHPLAAKIGKDVLDQGGNAMDAVI
                     AIQLALNVVEPFASGIGGAGYLLYYEQSTGSITAFDARETAPEHVDKQFYLDDSGEYK
                     SFFDMTTHGKTVAVPAIPKLFDYIHKRYAKLSLEDLINPAIELAIEGHAANWATEKYS
                     RQQHARLTKYHETAQVFTHENQYWREGDWIVQPELGKTFQILREQGFNAFYKGDIAKQ
                     LVNVVKACGGTIILEDLANYDIQIKAPISATFKDYDIYSMGPSSSGGITVIQILKLLE
                     HVDLPSMGPRSVDYLHHLIQAMHLAYSDRAQYLADDNFHEVPVQSLIDDDYLKARSTL
                     INSNKANIDIEHGVVSDCISHTDVEENHTETTHFCVIDKEGNIASFTTSIGMIYGSGI
                     TIPGYGVLLNTTMDGFDVVDGGINEIAPYKRPLSNMAPTIVMHHGKPILTVGAPGAIS
                     IIASVAQTLINVLVFGMDIQQAIDEPRIYSSHPNRIEWEPQFSQSTILALIARGHAME
                     HKPDAYIGDVHGLQVDLNTRDASGGADDTREGTVIGGDVLSIRKQPLPSPKIYDNDTH
                     RVYFNDMQLPLYAEQVRWMHDKYWVDESVIRIIFPEVSVHIEDLRSYEIAGKNYIDIA
                     WLARKKGYQVTLKDDSLYLTDETYHSVKANTNAYYRYDRDSITR"
     gene            complement(3206..4981)
                     /locus_tag="P6L01_001693"
     CDS             complement(3206..4981)
                     /locus_tag="P6L01_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDP3030760.1"
                     /translation="MKKIISIAIIVLALVLSGCGVPTKSEVAQKSSKVEVKGERPTIH
                     FLGQASYENDMNIVKDQLENAGFNVKMNIQPDYGSYRTQRQAGNYDIQIDDWMTVFGD
                     PNYAMTALFSSTGSNSLLKDKHVDQLLNKASTQNEADVKQTYKQIEDEVVFDKGYMAP
                     LYGSKKNLVYDNKVLDKNSVGLPNSRALIWQQFDYNNSRERDTRPLVMTQQDGEIPTL
                     DPIRSIAPSVYSINMNMYTRLLLLDENDHLTTKGSLSRDYAVNKDNKAFYFLLRDDDY
                     FAKVVNGQARNTGERVSAEDVKFSLDRARDKKSVPNNNTYNMHKHINDIKILKDEDID
                     QLRKEKDKDDKSIYDKLIKAYNVKSLTTDGQKVNNKDGIYQIVKITTDQSMPREVNYL
                     THSSAGILSKKFVNQVNQEYPKGYGDSSTIPANSDGKNALYASGAYIMTQKNAYQATF
                     QRNPGFNETEKGSYGSPKIKNITLKFNGDPNNALSELRNHSIDMLADVNQKHFDLIKS
                     DKNLSIIRKNGRKSVFLMLNIKKGIFKTHPNLRQAVVNAIDQDQFIKFYRGDKFKIAS
                     PITPLVDTGNEQRQDLEKVEKAINQ"
     gene            complement(4998..6161)
                     /locus_tag="P6L01_001694"
     CDS             complement(4998..6161)
                     /locus_tag="P6L01_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3030761.1"
                     /translation="MVKLTTKIASLKLFASYAIATYILVMLTSALNLFKGYVADTFYI
                     AETLLIILTIILIIILTTEQTWKHHDLWRRIVEVLLLLMALTGNVFTLLMFVSIRRYQ
                     RTSQIHSYNGWESFIRKTTRHRIAIIGLLILVYMLTLSIVSQFTFDTTLATKNQFNAL
                     LHGPSLAYPFGTDDFGRDLFTRVVVGTKLTFSISIISVVIAVIFGVLLGTIAGYFNHI
                     DNLIMRILDVVFAIPSLLLAVAIIASFGASIPNLIIALSIGNIPSFARTMRASVLEIK
                     RMEYVDAARITGENTWNIIWRYILPNAIAPMIVRFSLNIGVVVLTTSSLSFLGLGVAP
                     DVAEWGNILRTGSNYLETHSNLAIVPGVCIMFVVLAFNFIGDAVRDALDPRIH"
     gene            complement(6167..7645)
                     /locus_tag="P6L01_001695"
     CDS             complement(6167..7645)
                     /locus_tag="P6L01_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3030762.1"
                     /translation="MNCTFRLLTSNKFDMMLCLPIHKCVNLFYTFVYHSNLLKIILQG
                     VIIIITYLESFKSLYHKAFYKFVLSVLSLPIFLYAVIKFFFSAKRKNFYANNSEISEI
                     ERALHQKYKYLSQQKSSTQIHKEALKIFKAQSSNTSSKNIEQAHFSTYFENLLFHKFI
                     MIKVILALPMFILLTIYLQPLVRYIFERIVMAVIVIIGVIVSVFTILYFSPLDAAYSI
                     LGQNATKAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNEVVTKAVGERIP
                     ITIIVAVLALIVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILA
                     FSLKLDILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAK
                     GLSTTQVVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIP
                     DIPAVMGGVVYISIVISLANLIIDIFYALIDPKLRSEINERK"
     gene            7602..9194
                     /locus_tag="P6L01_001696"
     CDS             7602..9194
                     /locus_tag="P6L01_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3030763.1"
                     /translation="MSNLLEVNSLNVQFNYDETTVQAVKNVSFELRKKHILGIVGESG
                     SGKSITAKSILGLLPDYPDHTLTGEIIFNGQSLNNLSTSALQQIRGKDISMIFQDPLS
                     SLNPRLTIGKQITEVLFQHKRVSKSEAKSMTINILEKVGIKHATRQFDAYPHELSGGM
                     RQRVMIAMALILKPQILIADEPTTALDASTQNQLLQLMKSLYEYTETSIIFITHDLGA
                     VYQFCDDVIVMKDGSVVESGTVESIFKSPQHTYTKRLIDAIPDIHQTRPPRPLNNDIL
                     LKFDRVSVDYTSPSGSLYRAVNDINLAIRKGETLGIVGESGSGKSTLAKTVVGLKEVS
                     EGFIWYNELPLSLFKDDELKSLRQEIQMIFQDPFASINPRFKVIDVIKRPLIIHGKVK
                     DNDDIIKTVVSLLEKVGLDQSFLYRYPHELSGGQRQRVSIARALAVEPKVIVCDEAVS
                     ALDVSIQKDIIELLKQLQLDFGITYLFITHDMGVINEICDRVAVMKNGEIVELNNTED
                     IIKHPQSDYAKQLISEVAVIAK"
BASE COUNT         3092 a         1725 c         1477 g         3059 t
ORIGIN      
        1 taatactgat gtaaatattt ttcaaaatat attattatag tgttaatgaa gtattttgag
       61 aaacggtggg aatgtatgaa agcacataaa attttctggt taaacttagc agcaattatt
      121 attatttcaa ttgtagtcag tggcggtatg tttttggcaa tgaagtggga acaaattcat
      181 ttgaaagatg gtcttaaaaa agtactaagt acgtatccta ttaaaaactt agaaacgctt
      241 tatgaaattg atggtcacga taatccccat tatgaaaata atgatcaaga cacatggtat
      301 atagaatcct cttattcagt tgttggatca gatgagcttt taaaagaaga ccgtatgttg
      361 ttaaaggtag ataagaacac acataaaata actggtgaat atgacacaac tacaaacgat
      421 agaaaagatg ctactgactc gacatacaaa agttatccag taaaagtagt taataataaa
      481 atcgttttca caaaagacgt aaaagatccc gcgctgaaac aaaaaatcga aaacaatcaa
      541 tttttgattc aaagtggtga tttaacaagc attttaaata gcaatgattt gaaagtcaca
      601 catgatccta ccactgatta ttataattta tctggtaagt tgtcgaatga taatccaaac
      661 gttaaacaat taaaacgtag atataatatt cccagtaatg catcaacaaa ggtggaatta
      721 aagggaatga gtgatttaaa aggcaataat catcaagatc agaaacttta tttttatttt
      781 tcaagtcctg gaaaaaacca aattatatac aaagaaagcc ttacttataa taaattaagt
      841 gaacattaat acttatgatg taattataga aataaccaaa tcatttatta tcgtttaaca
      901 tattaaaatc acacattata aaaacgcctc acatcattca tttaattgta ccgaatcaca
      961 tcaagttaga ttttagacta actattgtgg tgtacatcaa aaacgaattt gtggggcgtt
     1021 ttatatttta caaaggcgac tagcgtttct tataacaact tcaacattac tacactcaag
     1081 catcactcta aagtttgaaa tcgaaccctt tcgttcaaca aaaatcaaaa tccacttcaa
     1141 actatatatc aaatacataa attatcttgt gatactatct cgatcatatc tatagtatgc
     1201 atttgtgttt gctttcactg aatgatatgt ttcatcagtt aagtataaac tgtcatcctt
     1261 taaggtaact tgataacctt tcttccgtgc caaccaggca atatctatat aattttttcc
     1321 ggcgatttca taacttctta aatcttcaat atgcacacta acttcaggga aaatgattct
     1381 aataacactt tcatcaaccc aatatttgtc atgcatccac cgcacttgtt cagcatataa
     1441 tggcaactgc atatcattga aatatactcg atgggtgtca ttatcgtata ttttcggact
     1501 aggtaatggt tgttttctaa ttgataatac atcgccacct atcactgtgc cttccctcgt
     1561 atcatcagca cctcccgacg cgtcacgtgt attcaagtca acctgtaacc catgtacatc
     1621 tccaatatag gcatctggtt tatgttccat tgcatgtcca cgtgcaatca atgctaatat
     1681 tgtagattgt gaaaattgag gctcccattc aatgcgatta ggatggctac tataaattct
     1741 aggttcatct atagcctgct gaatatccat gccaaacact aatacattga ttaatgtttg
     1801 cgcaacacta gcaatgatac ttatggcacc aggtgcacct actgttaata tcggcttccc
     1861 gtgatgcatc acaatcgttg gagccatgtt acttagtggt cgtttatatg gtgcaatttc
     1921 gttaatacca ccatctacta catcaaagcc atccattgtc gtattcaata acacaccgta
     1981 gcctggaatc gtgatacctg aaccataaat cataccaatt gatgtcgtaa atgaagcaat
     2041 attaccttcc ttatcaatca cacaaaaatg agttgtttcg gtatgatttt cttcaacatc
     2101 tgtatgacta atgcaatcag acacaacacc atgctctata tcaatatttg ctttattgct
     2161 attaatgagc gtactgcgtg cttttaaata atcgtcatca attaatgact gtacaggcac
     2221 ctcatgaaaa ttatcatccg ccaagtattg cgcgcgatca ctatatgcta aatgcatcgc
     2281 ttgtatcaaa tgatgcaagt aatcgacaga tcttggaccc atagatggta aatcgacatg
     2341 ttctaataac ttcaatattt gaatgaccgt gataccgcca gaactagatg gtcccattga
     2401 ataaatgtca tagtctttaa atgtcgcact gattggcgct ttaatctgaa tgtcataatt
     2461 ggctagatcc tctaaaatga ttgtcccacc acatgcttta acaacattga ctaattgttt
     2521 cgcaatgtca cctttataaa atgcattaaa cccttgttct cttaatattt gaaatgtctt
     2581 acctaattcg ggttgtacaa tccaatcacc ttcacgccaa tattgatttt catgcgtaaa
     2641 tacttgtgcc gtttcatgat actttgtcaa tcgtgcgtgt tgctggcgcg aatatttttc
     2701 agtagcccaa ttggctgcat gaccttcaat ggctagttca attgcaggat taattaaatc
     2761 ttccaatgac aatttagcat aacgcttgtg aatataatca aacagctttg gaattgctgg
     2821 cacagcgaca gttttaccat gtgtagtcat atcaaaaaat gatttatatt cgcctgaatc
     2881 atctagataa aattgtttgt ctacatgttc aggtgctgtc tcacgtgcat caaacgcagt
     2941 tatactgcca gtactttgct cataatatag caagtacccg gcaccaccaa tacctgatgc
     3001 aaatggttct accacattca atgccagttg aattgcaatc acggcatcca tggcgttgcc
     3061 accttgatct aatacatcct taccaatttt agccgcaaga ggatgtgata cggaaattaa
     3121 cccttcttta gatgtttttg tctgtttgtc atttaagtta atgaccatac tatatcctcc
     3181 tactttctgt taaatattta aaacattatt gattaatggc tttttctact ttttctaaat
     3241 cttgacgttg ctcgttacca gtatcgacaa gtggtgtaat cggtgatgca attttaaatt
     3301 tatcgccacg ataaaactta ataaattgat cctgatctat cgcattaact actgcttgtc
     3361 tcaagtttgg atgcgtctta aatatacctt ttttaatatt tagcattaaa aagactgact
     3421 tgcgtccatt tttgcgaata atgcttaaat ttttatccga cttaattaaa tcaaaatgtt
     3481 tttgattcac atctgccaac atatcaattg aatgatttct aagttctgac aatgcattat
     3541 tcggatcacc attaaacttc aatgtaatat ttttaatttt aggtgatcca taactacctt
     3601 tttctgtttc gttgaatcct ggattacgtt gaaacgttgc ttgatatgca tttttctgtg
     3661 tcataatgta tgcgccactt gcataaagcg cattttttcc atctgaattt gcaggaattg
     3721 tactgctatc cccatatcct tttggatatt cttgatttac ttgattaaca aattttttag
     3781 ataaaatgcc tgccgaagag tgtgttaagt aatttacctc tcgaggcatc gattgatctg
     3841 tcgtaatttt aacaatttga taaataccgt ctttattatt tactttttga ccatctgtcg
     3901 ttaacgattt gacgttataa gctttaatca acttatcata gattgattta tcatccttgt
     3961 ctttctcttt acgcaactga tcgatgtcct catcttttaa tatcttgatg tcatttatat
     4021 gtttgtgcat attgtaagta ttattgttag gcacagactt tttatcacgt gctctatcta
     4081 aagaaaactt aacatcttca gccgatacac gctctccagt attacgtgct tgtccattga
     4141 ccactttcgc aaaataatca tcatctctta acaagaaata aaatgcttta ttgtccttat
     4201 tcacagcata atcacgactt aacgaacctt tcgttgttaa gtgatcattt tcatctaata
     4261 ataataacct tgtgtacata ttcatattaa ttgaatatac tgacggcgca attgaacgta
     4321 ttggatccaa tgtaggaatt tcaccatctt gttgtgtcat cacaagtggc cgcgtatctc
     4381 gttctctact attgttgtaa tcaaattgtt gccatattaa tgcacgtgaa tttggcaatc
     4441 caacactatt tttatctaac actttattgt catatactaa attctttttt gatccatata
     4501 aaggcgccat atacccttta tcaaatacaa cttcatcttc aatttgctta tatgtttgtt
     4561 taacatctgc ttcattttga gtagaagctt tatttaacaa ctggtctaca tgtttatctt
     4621 tcaataaact atttgatcct gtagaactaa ataatgccgt catagcatag ttcgggtcac
     4681 caaacactgt catccagtca tcaatttgga tatcataatt gccggcttga cgttgtgtac
     4741 gatagctacc ataatctggt tggatattca tcttcacgtt aaatcctgca ttttccaatt
     4801 gatctttaac gatattcata tcattttcat aacttgcttg tcctaggaaa tgtattgttg
     4861 gtcgctcgcc tttcacttca actttcgatg acttttgagc cacttctgat ttcgtaggga
     4921 caccacaacc acttaatacc aacgctaaaa ctataattgc gatactaatg attttcttca
     4981 catctatccc taccttttta atgaattctt ggatctagtg catcacgcac tgcatcacct
     5041 ataaaattaa atgctaaaac gacgaacata atacaaacac caggtacaat agctaaatta
     5101 ctgtgcgttt ccaagtagtt actaccggta cgtaaaatgt tgccccattc agctacatca
     5161 ggtgcaacac caagtcctag gaaacttaaa ctacttgttg ttaatacaac cacacctata
     5221 tttaatgaaa aacgtacaat cataggcgca atcgcattcg gtaaaatata acgccatatg
     5281 atattccaag tgttttcacc agtgatacgt gctgcatcta catattccat gcgtttaatt
     5341 tctaaaacac tggcacgcat tgtccgtgca aatgatggta tattaccgat acttaaagca
     5401 ataattaaat ttggaatact tgctccaaat gatgcaataa ttgccaccgc taacaataat
     5461 gatggaattg caaacactac atctaaaatt cgcattatta aattatcaat atgattaaaa
     5521 taacctgcga tagtgcctag taacacacca aaaataactg caataactac tgaaataatt
     5581 gaaattgaaa atgtcagctt cgttcctaca actacgcgtg taaataagtc tctaccgaaa
     5641 tcatcagtac caaacggata ggctagactc ggtccatgta acagtgcatt gaactgattt
     5701 ttagtagcca atgtcgtatc aaatgtaaat tgtgacacaa ttgataatgt cagcatgtag
     5761 actaaaataa gtaacccgat aatcgcaata cgatgtctag tagtttttcg tataaacgat
     5821 tcccacccgt tataactatg tatttgcgat gtacgttggt aacgtctaat acttacaaac
     5881 attaataatg taaatacgtt gcctgttaat gccatcaaca ataacaacac ttcgacgata
     5941 cgtcgccata ggtcatgatg cttccatgtt tgttccgttg ttaaaatgat aattaaaatg
     6001 atggttaaaa tgattagcaa tgtttcagca atatagaacg tatcggccac ataaccttta
     6061 aaaagattta atgcactcgt taacataact aaaatataag ttgctatagc ataacttgcg
     6121 aatagtttta aggaagctat ctttgttgta agttttacca tatgcctcac ttcctttcgt
     6181 taatttcact acgtaatttt ggatcgatta aagcataaaa tatatcaata attaagtttg
     6241 ctaaagatat tacaattgat atatatacga ccccacccat gactgctggg atatcaggta
     6301 ttagttgttt ttggacgata taacgaccga taccattaat gttaaatact tgttccgtca
     6361 ctgctgaacc gcctagtaac tctgccacta gaagaccaac taacgttaca attggaataa
     6421 tggcattttt caaaatatgt ttaataacaa cttgtgtcgt cgataatcct tttgcataag
     6481 cagttaaaac ataatcgctg cgcattactt caagtacaga agaccttgtc atacgcgtga
     6541 tagaagcagc aatacttgtt ccaatgacaa gtacaggtaa aatcaacgat attggatgtt
     6601 ctggcatata agatggtggc aaaatatcca atttcaatga gaacgctaaa atgaataata
     6661 gcccttgcca gaaacttgga atagataaac caattaatgc aattatcatt aacgtgatat
     6721 caagccaact atttcgcttc atcgcactga taataccaat tggtattgca ataattaatg
     6781 ccacaattag cgctaatact gcgacaatta ttgtaattgg aattctttcg ccaactgctt
     6841 tagtcacaac ctcattccct ttgtaagtcg tacctaagtc aaaggtaaaa acacccttga
     6901 tggtatccca caattgaata aaataaggtt cgttaagatg atgtaataca ttgaattgat
     6961 gtatctgtgc ctttgttgca ttttgtccca gtatgctata agccgcatca agcggtgaaa
     7021 aatacagaat ggtaaacaca ctgacaataa caccaatgat gacaatcaca gccatgacaa
     7081 ttcgctcaaa aatatatcta actaatggct gtaaataaat agtcaataag atgaacatcg
     7141 gcaaggccaa tattactttg atcatgatga acttatgaaa taataaattt tcaaagtatg
     7201 ttgaaaaatg tgcttgttca atattctttg aactcgtatt agaactttgt gccttgaata
     7261 tttttaatgc ttctttatgt atttgtgtgg atgacttttg ctgcgataaa tatttatatt
     7321 tttgatgtaa cgctcgttca atttctgaaa tttcagaatt attagcgtaa aaattttttc
     7381 tcttagcaga aaagaaaaac tttatcactg catataaaaa tattggcaag cttaataccg
     7441 ataatacaaa cttgtaaaat gccttgtgat acaacgactt aaaagattct aaataagtaa
     7501 ttatgataat cactccttgt aaaataatct tgagtagatt actatgatat acaaaagtat
     7561 agaataaatt tacacatttg tgaataggga ggcacaacat catgtcaaat ttattagaag
     7621 tcaacagtct gaatgtacaa ttcaattatg atgaaactac agttcaagcg gtaaaaaatg
     7681 tctctttcga attacgaaaa aaacatatcc taggtattgt tggtgaatca ggatcaggaa
     7741 aaagtattac tgctaaatct attttagggc tactaccaga ttatccagat cacacattaa
     7801 caggagaaat tatttttaat gggcaatcgt taaataattt atcaacttca gcgttacaac
     7861 aaattcgagg taaggatatt tcaatgattt ttcaagatcc actctcttcg ttgaatccaa
     7921 gattaacgat tggcaaacaa attacagaag tactatttca acataaacgt gtatctaaat
     7981 ctgaagcaaa gtcgatgaca ataaacattt tagaaaaagt aggtataaaa catgcaactc
     8041 gacaatttga tgcttatcca catgaacttt ctggtggtat gcgtcaacgt gtcatgatag
     8101 caatggcatt gattttaaag ccacaaattt taatcgcaga tgaaccaaca acggcattag
     8161 atgccagtac acaaaatcaa ttactgcagt taatgaagtc cctttatgag tacacagaaa
     8221 catctattat ttttatcact cacgatttag gcgctgtgta tcaattttgc gacgatgtga
     8281 ttgtaatgaa agatggaagt gtcgttgaaa gtggcacggt tgaaagtatt tttaaatcgc
     8341 cacaacatac ctatacaaaa cgcttaatag atgcgattcc tgatattcat caaacgcgtc
     8401 cgccaagacc gttaaacaat gatattttat taaaattcga tcgcgtgagc gtggattaca
     8461 catcaccgag tggcagccta taccgagcag ttaatgatat taacttggct attagaaaag
     8521 gcgaaacatt aggcattgtc ggtgaatcag ggtcagggaa atcgacatta gctaagacgg
     8581 tcgtcggtct aaaggaagtg tcagaaggct ttatttggta taacgaatta ccattaagtt
     8641 tatttaaaga tgatgaattg aaatctttac gacaagagat acaaatgatt tttcaagatc
     8701 cattcgcatc tattaatcca agatttaaag tcattgatgt gattaaacga ccactaatca
     8761 ttcatgggaa agtcaaagat aatgatgaca ttattaaaac tgtcgtatcg ttgttagaaa
     8821 aggttggcct agatcaaagt ttcttatatc gctatccaca cgaattatct ggtgggcaac
     8881 gtcagcgtgt aagtatcgcg agagcacttg ctgtagaacc taaagtgatt gtttgcgacg
     8941 aggcagtgtc cgctttagac gtttcaattc aaaaagatat catcgagtta ttaaaacaat
     9001 tacagttaga cttcggcatc acttatttat tcatcacaca tgacatgggt gttatcaatg
     9061 aaatatgtga tcgcgttgca gttatgaaaa atggcgaaat cgttgaactg aataacacag
     9121 aagatattat caaacatccg caatcagact atgcaaagca acttatttca gaagtagcag
     9181 ttattgctaa ataaaagtca tgcgttgtgt aactttatca ctgtatggtc tggaataaat
     9241 cgggcaacct ctgatgtgtt atgttagatg actttgtcat actaactata ttgtgctaag
     9301 ttagggctcg gcaccatatc aagttttaag tgccttgcca tgatttaagg gtc
//
LOCUS       DAOBBH010000100         9132 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.100, whole genome shotgun sequence.
ACCESSION   DAOBBH010000100 DAOBBH010000000
VERSION     DAOBBH010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9132
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.100"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..532
                     /locus_tag="P6L01_001697"
     CDS             <1..532
                     /locus_tag="P6L01_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDP3030764.1"
                     /translation="LETIIKAVENLEPVEGRLEVLDPSLPIDLIIDYAHTADGMNKLI
                     DAVQPFVKQKLIFLVGMAGERDLTKTPEMGRVACRADYVIFTPDNPANDDPKMLTAEL
                     AKGATHQNYIEFDDRAEGIKHAIDIAEPGDTVVLASKGREPYQIMPGHIKVPHRDDLI
                     GLEAAYKKFGGGPVDQ"
     gene            513..773
                     /locus_tag="P6L01_001698"
     CDS             513..773
                     /locus_tag="P6L01_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueH family protein"
                     /protein_id="HDP3030765.1"
                     /translation="MALLINKRFIDEGKTIDVYLFEALNNQIIIAIPDWFWSYQMAMT
                     LDEETCFEAILMQLFVFKEEEEAESIASQLTDWIETYKKEKD"
     gene            773..2335
                     /locus_tag="P6L01_001699"
     CDS             773..2335
                     /locus_tag="P6L01_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDP3030766.1"
                     /translation="MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVK
                     GKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMA
                     VDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLN
                     IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSAFE
                     QAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQ
                     TKEDVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQ
                     KITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEI
                     FMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKN
                     EYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFE
                     EKFLEIKLYSLL"
     gene            2637..3440
                     /locus_tag="P6L01_001700"
     CDS             2637..3440
                     /locus_tag="P6L01_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDP3030767.1"
                     /translation="MLMDPSLILPYLWVLVVLVFLEGLLAADNAIVMAVMVKHLPPEQ
                     RKKALFYGLLGAFVFRFLALFLISIIANFWFIQAAGAVYLIYMSIKNLWQFFKHPEIE
                     SPEAGDDHHYDESGEEIKASNKSFWGTVLKIEFADIAFAIDSMLAALAIAVTLPKVGI
                     HFGGMDLGQFVVMFLGGMIGVILMRYAATWFVELLNKYPGLEGAAFAIVGWVGVKLVV
                     MVLAHPDIAVLPEHFPHGVLWQSIFWTVLIGLVIIGWLGSVVKNKKSHK"
     gene            3659..5983
                     /locus_tag="P6L01_001701"
     CDS             3659..5983
                     /locus_tag="P6L01_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDP3030768.1"
                     /translation="MEWTLVDIGKKHVIPKSQYRRKRREFFHNEDREENLNQHQDKQN
                     IDNTTSKKADKQIHKDSIDKHERFKNSLSSHLEQRNRDVNENKAEESKSNQDSKSAYN
                     RDHYLTDDVSKKQNSLDSVDQDTEKSKYYEQNSEATLSTKSTDKVESTEMRKLSSDKN
                     KVGHEEQHVLSKPSEHDKETRIDSESSRTDSDSSMQTEKIKKDSSDGNKSSNLKSEVI
                     SDKSNTVPKLSESDDEVNNQKPLTLPEEQKLKRQQSQNEQTKTYTYGDSEQNDKSNHE
                     NDLSHHTPSISDDKDNVMRENHIVDDNPDNDINTLSLSKIDDDRKLDEKIHVEDKHKQ
                     NADSSETVGYQSQSTASHRSTEKRNISINDHDKLNGQKTNTKTSANNNQKKATSKLNK
                     GRATNNNYSDILKKFWMMYWPKLVILMGIIILIVILNAIFNNVNKNDRMNDNNDADAQ
                     KYTTTMKNANNTVKSVVTVENETSKDSSLPKDKASQDEVGSGVVYKKSGDTLYIVTNA
                     HVVGDKENQKITFSNNKSVVGKVLGKDKWSDLAVVKATSSDSSVKEIAIGDSNNLVLG
                     EPILVVGNPLGVDFKGTVTEGIISGLNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNS
                     GGAVVNREGKLIGVVAAKISMPNVENMSFAIPVNEVQKIVKDLETKGKIDYPDVGVKM
                     KNIASLNSFERQAVKLPGKVKNGVVVDQVDNNGLADQSGLKKGDVITELDGKLLEDDL
                     RFRQIIFSHKDDLKSITAKIYRDGKEKEINIKLK"
     gene            6000..7358
                     /locus_tag="P6L01_001702"
     CDS             6000..7358
                     /locus_tag="P6L01_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDP3030769.1"
                     /translation="MSIFSQFLKRSSPQQGIVLYYIVAIVIAFLLLNLPYVHKPGVEV
                     NPIDTLFVAVSGISVTGLSPISIVDTYSTFGQLIILVILNIGGIGVMAIGTMLWVVLG
                     KHIGIRERQLIMLDNNKNTMSGTVKLIIDIVKSIFVIELVGAMLLAFYFYRDNPDLKY
                     AIMQGVFVSISATTNGGLDITGKSLIPYAHDYFVQAIVIFLIILGSIGFPVLLEVKAY
                     IQNRVTNFRFSLFTKITTSTYLFLFIVGVLAILLFEHNHAFKGLSWHQSLFYSLFQSA
                     TTRSAGLQTIDVTTLSDPTNIIMGILMFIGSSPSSVGGGIRTTTFAILILFLINFSNN
                     ADKTSIKVYNREVHIMDIQRSFAVFTMATILTFLGMLIISATENGKLTFLQVFFEVMS
                     AFGTCGLSLGVTSDISDISKVVLMVLMFIGRVGLISFIIMIAGRREPDKFHYPKERIQ
                     IG"
     gene            7497..9008
                     /locus_tag="P6L01_001703"
     CDS             7497..9008
                     /locus_tag="P6L01_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDP3030770.1"
                     /translation="MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQIRAQN
                     HRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFL
                     TRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
                     EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLM
                     EELGVIDLMITAHQHQTIVGQDHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDS
                     KVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQL
                     LHDAIQLAFDNDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDI
                     LEYSYSHLDFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLTKSYRV
                     TMTDYCYRNYKNYLKNAIIHESYDETMSTLIAEKLRDPNYRISCSDNFVVKNR"
BASE COUNT         3286 a         1211 c         1759 g         2876 t
ORIGIN      
        1 tttagaaacg attattaaag ctgttgaaaa tttagaacct gttgaagggc gattagaagt
       61 tttagatcct tcgttaccta ttgatttaat tatcgattat gcacatacag ctgatggtat
      121 gaacaaatta atcgatgcag tacagccttt tgtaaagcaa aagttgatat ttttagttgg
      181 tatggcaggc gaacgtgatt taactaaaac gcctgaaatg gggcgagttg cctgtcgtgc
      241 agattatgtc attttcacac cggataatcc ggcaaatgat gacccgaaaa tgttaacggc
      301 agaattagcc aaaggtgcaa cacatcaaaa ctatattgaa tttgatgatc gtgcagaagg
      361 gataaaacat gcaattgaca tagctgagcc tggggatact gtcgttttag catcaaaagg
      421 aagagaacca tatcaaatca tgccagggca tattaaggtg ccacatcgag atgatttaat
      481 tggccttgaa gcagcttaca aaaagttcgg tggtggccct gttgatcaat aaaagattta
      541 ttgatgaagg taaaactatt gatgtttatt tattcgaagc attaaataac cagataatca
      601 ttgctatacc agattggttt tggtcatatc agatggcaat gacattagat gaagaaactt
      661 gttttgaagc aatactcatg caattgtttg tttttaaaga agaggaagag gcagaatcga
      721 ttgcatcaca actaacagat tggatagaaa catataaaaa ggagaaagac taatgaactt
      781 aaagcaagaa gttgagtcta gaaagacttt tgcgattatt tcacatcccg atgcagggaa
      841 aacaacgtta actgaaaaac tattgtactt cagtggtgct attcgtgaag cgggtacagt
      901 taaagggaag aagactggta aatttgcgac aagtgactgg atgaaagttg aacaagagcg
      961 tggtatttct gtaactagtt cagtaatgca atttgattac gatgattata aaatcaatat
     1021 cttagataca ccaggacatg aagacttttc agaagatacg tatagaacat taatggcagt
     1081 tgacagtgct gtcatggtca tagactgtgc aaaaggtatt gaaccacaaa cattgaagtt
     1141 atttaaagtt tgtaaaatgc gtggtattcc aatctttaca ttcattaata aattagaccg
     1201 agtaggtaaa gaaccatttg aattattaga tgaaatcgaa gagacattaa atattgaaac
     1261 ataccctatg aattggccaa ttggtatggg acaaagtttc tttggcatca ttgatagaaa
     1321 gtctaaaaca attgaaccat ttagagatga agaaaatata ttacatttga atgatgattt
     1381 tgagttggaa gaagatcatg caattacaaa tgatagtgct tttgaacaag cgattgaaga
     1441 attaatgttg gttgaagaag cgggtgaagc ctttgataat gacgcgctgt tgagtggaga
     1501 cttaacacct gtatttttcg gttcagcttt agctaacttt ggtgtacaaa atttcttaaa
     1561 tgcatatgtt gattttgcgc caatgccaaa tgcgagacaa acaaaagaag acgttgaagt
     1621 aagcccgttt gatgattcat tttcaggatt tatctttaaa attcaagcca acatggaccc
     1681 taaacaccgt gatagaattg cctttatgcg tgtcgttagt ggtgcatttg aacgtggtat
     1741 ggatgttact ttgcaacgta ctaataaaaa gcaaaagatc acacgttcaa cgtcatttat
     1801 ggcagacgat aaagaaactg tgaatcatgc tgtagcaggc gatatcattg gactatatga
     1861 tactggtaat tatcaaattg gagatacttt agttggtgga aaacaaacct acagtttcca
     1921 agatttacca caatttacgc cagaaatttt tatgaaagtt tctgctaaaa acgtcatgaa
     1981 acagaagcat ttccataaag gtattgaaca attagtacaa gaaggtgcga ttcaatacta
     2041 taaaacatta cacacaaacc aaattatttt aggtgctgtt ggtcagttac aatttgaagt
     2101 tttcgaacat agaatgaaaa acgaatataa tgttgatgtt gttatggagc cagtaggccg
     2161 taaaattgca cgttggattg aaaatgaaga ccaaattaca gataagatga acacatcaag
     2221 atcgatttta gtgaaagata gatatgacga tttagtattc ttatttgaaa atgaatttgc
     2281 aacaagatgg tttgaagaga aattccttga aattaaattg tatagtttac tttaacagct
     2341 caattgtata atcgaatttg ttacattaaa aataattgtt tcgttgaaga aaaataaatt
     2401 gtatatttta aaagaaaaag gtatactatg atgtatcaaa tgaataacct atggcatttt
     2461 gtcagagggg agtaacttaa gaatcatgac cgtataaatg attcgacact ttatcgtcat
     2521 tacgaagata tcttccggta aagtgggcaa tttaaattgc ttagtgagac ctttgctatt
     2581 tatttagcat aggtcttttt gtttgtactt aacttattta tttaaaggag ttgtacatgt
     2641 taatggatcc aagtttgatc ttaccttatt tatgggtact tgtcgtttta gtatttttag
     2701 aaggcttatt agcagcagat aacgcgattg ttatggctgt aatggttaag cacttaccac
     2761 ccgaacaacg taaaaaagct ttgttttacg gtttgttagg tgcatttgta tttagatttt
     2821 tagcattatt cttaattagt attatcgcga acttttggtt tattcaagct gcaggagcgg
     2881 tttacttaat ttatatgtca atcaaaaatc tgtggcagtt ctttaaacac ccagaaattg
     2941 aaagtcctga agctggagat gatcatcatt atgatgaatc tggtgaagag attaaagcaa
     3001 gtaacaaatc attctgggga actgtgttga aaatagaatt tgcagatatc gcatttgcca
     3061 ttgattctat gcttgctgct ttagctattg ctgtaacact tcctaaagtt ggtattcact
     3121 ttggtggtat ggacttaggt cagttcgtag tcatgttcct aggtggaatg attggtgtta
     3181 ttctaatgcg ttatgcagca acatggtttg tagagctatt aaacaaatat ccaggacttg
     3241 aaggtgcagc cttcgcgatc gttggttggg taggtgttaa attagttgtc atggtattag
     3301 cgcacccaga catcgctgta ttgcctgagc acttcccaca tggcgtatta tggcaatcta
     3361 ttttctggac agtactaatt ggattagtaa ttatcggttg gttaggttca gttgttaaaa
     3421 ataaaaaatc gcataaataa ttgatgtgaa gcggacaatc ttaatttagt ttaaggttgt
     3481 cctttttcat ttaattgagt gatttatgaa aaatggattt tgaagaatgt gaatcaaaag
     3541 atgcgatata gtattaagaa aatgtgcctt ttatatttag cattttttca atagaaatta
     3601 tatagatttt aaagcaaatt aggtgttaat gtgtcataat gataagtgat tttattgaat
     3661 ggagtggaca ttagtggata ttggtaaaaa acatgtaatt cctaaaagtc agtaccgacg
     3721 taagcgtcgt gaattcttcc acaacgaaga cagagaagaa aatttaaatc aacatcaaga
     3781 taaacaaaat atagataata caacatcaaa aaaagcagat aagcaaatac ataaagattc
     3841 aattgataag cacgaacgtt ttaaaaatag tttatcatcg catttagaac agagaaaccg
     3901 tgatgttaat gagaataaag ctgaagaaag taaaagtaat caggatagta agtcagcata
     3961 taacagagat cattatttaa cagacgatgt atctaaaaaa caaaattcat tagattcagt
     4021 ggaccaagat acagagaaat caaaatatta tgagcaaaat tctgaagcga ctttatcaac
     4081 taaatcaacc gataaagtag aatcaactga aatgagaaag ctaagttcag ataaaaacaa
     4141 agttggtcat gaagagcaac atgtactttc taaaccttca gaacatgata aagagactag
     4201 aattgattct gagtcttcaa gaactgattc agacagctcg atgcagacag agaaaataaa
     4261 aaaagacagt tcagatggaa ataaaagtag taatctgaaa tctgaagtaa tatcagacaa
     4321 atcaaataca gtaccaaaat tgtcggaatc tgatgatgaa gtaaataatc agaagccatt
     4381 aactttaccg gaagaacaga aattgaaaag acagcaaagt caaaatgagc aaacaaaaac
     4441 ctatacatat ggtgatagcg aacaaaatga caagtctaat catgaaaatg atttaagtca
     4501 tcatacacca tcgataagtg atgataaaga taacgttatg agagaaaatc atattgttga
     4561 cgataatcct gataatgata tcaatacact atcattatca aaaatagatg acgatcgaaa
     4621 acttgatgaa aaaattcatg ttgaagataa acataaacaa aatgcagact cgtctgaaac
     4681 ggtgggatat caaagtcagt caactgcatc tcatcgtagc actgaaaaaa gaaatatttc
     4741 tattaatgac catgataaat taaacggtca aaaaacaaat acaaagacat cggcaaataa
     4801 taatcaaaaa aaggctacat caaaattgaa caaagggcgc gctacgaata ataattatag
     4861 tgacattttg aaaaagtttt ggatgatgta ttggcctaaa ttagttattc taatgggtat
     4921 tattattcta attgttattt tgaatgccat ttttaataat gtgaacaaaa atgatcgcat
     4981 gaatgataat aatgatgcag atgctcaaaa atatacgaca acgatgaaaa atgccaataa
     5041 cacagttaaa tcggtcgtta cagttgaaaa tgaaacatca aaagattcat cattacctaa
     5101 agataaagca tctcaagacg aagtgggatc aggtgttgta tataaaaaat ctggagatac
     5161 gttatatatt gttacgaatg cacacgttgt cggtgataaa gaaaatcaaa aaataacttt
     5221 ctcgaataat aaaagtgttg ttgggaaagt gcttggtaaa gataaatggt cagatttagc
     5281 tgttgttaaa gcaacttctt cagacagttc agtgaaagag atagctattg gagattcaaa
     5341 taatttagtg ttaggagagc caatattagt cgtaggtaat ccacttggtg tagactttaa
     5401 aggcactgtg acagaaggta ttatttcagg tctgaacaga aatgttccta ttgatttcga
     5461 taaagataat aaatatgata tgttgatgaa agctttccaa attgatgcat cagtaaatcc
     5521 aggtaactcg ggtggtgctg tcgtcaatag agaaggaaaa ttaataggtg tagttgcagc
     5581 taaaattagt atgccaaacg ttgaaaatat gtcatttgca atacctgtta atgaagtaca
     5641 aaagattgta aaagatttag aaacaaaagg taaaattgac tatcccgatg taggtgttaa
     5701 aatgaagaat attgccagtc taaatagttt tgaaagacaa gcagttaaat tgccaggaaa
     5761 agttaagaac ggtgttgttg tagatcaagt tgacaacaat ggtttagcag atcaatctgg
     5821 tctgaaaaaa ggtgatgtaa ttactgaatt agatggcaaa cttttagaag atgatttacg
     5881 ctttaggcag attatattta gtcataaaga tgacttgaaa tcaattacag cgaagattta
     5941 tagagatggt aaagagaaag aaattaatat taaactaaaa taatgttgag gtgaacaacg
     6001 tgtcaatttt tagccagttt ttaaaaagat caagccctca acaaggtatt gtattgtact
     6061 atatcgtcgc aattgtcatt gcatttttat tattaaactt accgtatgtt cataaaccag
     6121 gtgtagaagt aaatccaatt gacacattat ttgttgccgt atccggaatt agtgttactg
     6181 gattgtctcc gataagtatt gtcgatacct attctacatt tggacaatta attatcctcg
     6241 tgatattaaa tattggtgga attggcgtca tggcaattgg tacgatgtta tgggtggtac
     6301 taggtaaaca tattggaatt agagaacgtc agttaattat gttagataat aacaaaaaca
     6361 caatgagtgg taccgtcaaa ttgattattg atattgtaaa atcaatattt gtaatcgaac
     6421 tcgtaggagc catgttatta gcattttact tttatcgaga taatccagat ttaaaatatg
     6481 caatcatgca aggtgttttt gtttctattt ctgccactac caatggtgga ttagatatta
     6541 caggtaagtc attaattcct tatgcacatg attattttgt acaagcgata gttatatttt
     6601 taataatttt aggatcaatc ggcttcccag tattattaga agttaaagct tatattcaaa
     6661 atagggttac taattttaga ttttcattat ttactaaaat tacgacatca acatatttat
     6721 tcctatttat tgttggggta ttagccattc tattatttga acataaccat gcgttcaaag
     6781 gtttaagttg gcatcaatcg ttattctatt cgctgtttca atcagcgact acaagaagtg
     6841 cgggtcttca aacaattgat gtgacaacac taagtgaccc cactaatatt atcatgggta
     6901 ttttaatgtt tataggatct tcgccaagtt cggttggtgg cggtattcgt acaacaactt
     6961 tcgctatttt aattttgttt ttaattaact ttagtaataa tgccgataaa acatccatta
     7021 aagtttacaa tagagaagta cacattatgg atattcaacg ttcatttgca gtatttacaa
     7081 tggcgacaat tttaacattt ttaggaatgc taattatatc agctactgaa aatggtaagc
     7141 ttacattttt acaagtattt tttgaagtca tgtctgcatt tggaacttgt ggactatcgc
     7201 ttggtgtcac aagtgatatt agtgatattt ctaaggtcgt actaatggta ctcatgttta
     7261 taggacgtgt tggcttaata tcatttatca ttatgatagc aggacgtcga gaaccagata
     7321 aattccatta tccaaaagaa cgtattcaaa taggataata taatagcaat ctaagtttag
     7381 ttaatataga ttttaactgg aacttagatt gcttttttag tttgtatttt taacttattt
     7441 tataagacga ttggttttca aaatggtaaa ctagtaacaa tgagaggtgt aacatgatgg
     7501 aaaaaaatga aaacattaat gtagagattt taactacgtc agatatgcat agtcatttct
     7561 taaatggtga ttatggttca aatatttata gagctggtac ttatgttaac caaataagag
     7621 cacaaaatca tcgcgtcatt ttattagata gtggcggaag tttagctggc tcgttagcgg
     7681 cctattatta tgctattgtt gcaccttata aacgacatcc aatgataaag ttaatgaaca
     7741 gaatgcatta tgatgctagc ggtgtgagtc caagtgaatt caagtttggt ttatcatttt
     7801 taactcgttc aattgctttg gcacgttttc catggttatc agcaaatatt gaatacaatg
     7861 ttactaagga gccttatttt tcaactccat attgtattaa acattttggt gacttaaaaa
     7921 ttgctatcgt aggcgtcaca gcagatggtt taatggaaaa tgagtattct gaaatggagc
     7981 aagatgtatc tattgaaaag acattagtgg catcaaaacg ttggattaga tatatccatg
     8041 aagttgaaga gccagatttt ttgattgtaa tttatcatgg tggattgaat aaaattagta
     8101 atagtacgaa aaataaaaag gcaagttcga atgaagctga aaaattaatg gaagaactcg
     8161 gtgttataga tttaatgatt acagctcatc agcatcaaac aatagtaggt caagatcatg
     8221 aaacgtatta tgttcaggct ggtcaggatg ccaaagagct tgtacatctt tcgattaatt
     8281 ttaaaaagag aacaacaact tatgatgttg aaagcattga ttctaaagtg attgacttaa
     8341 atgagtatga agaggatcaa gaattattag atttaacatt ctatgataga aaagcagtgg
     8401 cttattggtc acaggaaatc ataagtgata aaggtttgat gttatcagtg aatgggttac
     8461 aagatttagt ctgtcaaaca catccatttt cgcaattatt acatgatgca attcagcttg
     8521 catttgataa tgatataaca tgtgtccacg tgcctatgaa cggagagaag gggttgagtg
     8581 gacagattcg aaatgaagat ttgtatcatg catacccata tccagataag ccaatggata
     8641 tgacaattag tggtcaaaat atcaaagata tattggagta tagttattca catttagatt
     8701 ttgttaacga gcaattaagc ttaacaatta ttgatgaaac gttatgtaca atgtggcaag
     8761 gattcaatta tgagattgat atgaatcaag aacctgggca acgagtaatg ttagatcaaa
     8821 ttgatttgac taagagttat agagttacaa tgactgacta ttgttatcgt aactacaaga
     8881 attatttaaa aaatgctatt atacatgaat cgtacgatga aacaatgagt acattaattg
     8941 cagagaagtt aagagatccg aattatcgta tttcatgtag tgataatttt gtagttaaaa
     9001 acaggtaaat attgctatgt aataaattaa ttataagttc aacaagtata aaaacctttc
     9061 ttagagaaga ttatctagga aaggttttta gaattttaaa attgatataa aagaacagtg
     9121 aaattctcat tc
//
LOCUS       DAOBBH010000101         8991 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.101, whole genome shotgun sequence.
ACCESSION   DAOBBH010000101 DAOBBH010000000
VERSION     DAOBBH010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8991
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.101"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     regulatory      255..389
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            451..996
                     /locus_tag="P6L01_001704"
     CDS             451..996
                     /locus_tag="P6L01_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDP3030771.1"
                     /translation="MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSL
                     LATFTFGPVAGIVVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTK
                     SLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKG
                     IVISIVFILLYRRLANFLKRI"
     gene            complement(1102..1350)
                     /locus_tag="P6L01_001705"
     CDS             complement(1102..1350)
                     /locus_tag="P6L01_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDP3030772.1"
                     /translation="MAKYTIVDMDTCIACGACGAAAPDIYDYDDEGIAFVILDDNQGT
                     AEVPEELYEDMEDAIDGCPTDSIKIADESFDGDALKFE"
     gene            1458..2411
                     /locus_tag="P6L01_001706"
     CDS             1458..2411
                     /locus_tag="P6L01_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDP3030773.1"
                     /translation="MQHIIKTALQQTFNYKTNKSIYNILVGKKSHQTFFDACSQQQLS
                     LYHSLPLLKYPSFELFLEKINEFNAEMEIMLHPRYTFESMGQTFQAIQLLVQTMSNTK
                     QHVFHFVPISQNNKIQETVKIVYNYIKENQLQIDFENELHNLFKAITLKGPCYLHYYL
                     QGYDEPMYTRQQVSLIEKLSQQQLFEYEMNNLVTMMFELESGEYTILSKIIMKPTLLN
                     QTYITYTKLLEQFTMEDIAAQQQVKINTIEDHVLEILIKGYMSNYDDYVELEDQLQFL
                     NFYQQHRGERLKFYKEQFDTLSYFQLKVLIVGFERGDLNVA"
     gene            2401..3780
                     /locus_tag="P6L01_001707"
     CDS             2401..3780
                     /locus_tag="P6L01_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDP3030774.1"
                     /translation="MLHDILRNKFGFENFKPGQQEIIESIMSQQHTLGILPTGSGKSL
                     CYQIPTYLSGKPTLIISPLISLMDDQVMQLKINGEKRVTCIHSGMDEIEKKHNIKCLL
                     HSRFIFLSPEFLLQPSNFKLISMIDFGMIVLDEAHCLSEWGYDFRPHYALIGKVTKHF
                     KEAVVLALTATAPPHLQDDLTEMLAIQFNVIKTTMNRPNISFKHLNFHDDEDKIEWLL
                     PFLQQSGPTIIYVSSKKMCLNLAQLIYDSGFLTGIYHGDMNYQERHTVQQQFLNNDIP
                     IIVATSAFGMGINKKDIRTIIHFHLSTSPSNYIQEIGRAGRDGELSQAISLFQPDDKY
                     ILETLLFADMITEEDVQNFEIGEFLAPDKQDVLTTLHSFYSIGALKQIFKQSFKRKQL
                     GFFRMIGYCKLDQCRRKYLLEFFGEYPPAQDRCCDNDSNITDIVILNKKKVIRSIGFD
                     EKLQNLFLR"
     gene            3933..5393
                     /gene="ebpS"
                     /locus_tag="P6L01_001708"
     CDS             3933..5393
                     /gene="ebpS"
                     /locus_tag="P6L01_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elastin-binding protein EbpS"
                     /protein_id="HDP3030775.1"
                     /translation="MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENT
                     EQQFPPRNAQRRKRRRDLATNHNKQVHNESQTSEDNVQNEAGTIDDRQVESSHSTESQ
                     EPSHQDSTPQHEEEYYNKNAFAMDKSHPEPIEDNDKHETIKDAENNTEHSTVSDKSKA
                     EQSQQPKPYFATGANQANTSKDKHDDVTVKQDKDESKDHHSGKKGAAIGAGTAGVAGA
                     AGAMGVSKAKKHSNDAQNKSNSDKSNNSTEDKASQDKSKDHHNGKKGAAIGAGTAGLA
                     GGAASKSASAASKPHASNNASQNHDEHDNHDRDKERKKGGMAKVLLPLIAAVLIIGAL
                     AIFGGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKEDQD
                     KATKDESDNDQNNANQANNQAQNNQNQQQANQNQQQQQQRQGGGQRHTVNGQENLYRI
                     AIQYYGSGSPENVEKIRRANGLSGNNIRNGQQIVIP"
     gene            5518..5634
                     /locus_tag="P6L01_001709"
     CDS             5518..5634
                     /locus_tag="P6L01_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030776.1"
                     /translation="MLFNKVLSRILAYQLNFLNSHQKDKTPQSTNLTRENKK"
     gene            5795..6781
                     /locus_tag="P6L01_001710"
     CDS             5795..6781
                     /locus_tag="P6L01_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HDP3030777.1"
                     /translation="MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNY
                     PTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKK
                     MNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
                     IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNA
                     NVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYN
                     KETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQNMLAKKQTPLES"
     gene            complement(6896..7864)
                     /locus_tag="P6L01_001711"
     CDS             complement(6896..7864)
                     /locus_tag="P6L01_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDP3030778.1"
                     /translation="MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQI
                     DELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILG
                     IEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTK
                     THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGD
                     IFSFYSREGIDGMVIEALGQGNIPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAY
                     DGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKAEIKSYFEL"
     gene            7941..8600
                     /locus_tag="P6L01_001712"
     CDS             7941..8600
                     /locus_tag="P6L01_001712"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDP3030779.1"
                     /translation="MKAINIALDGPAAAGKSTIAKRVASELSMIYVDTGAMYRALTYK
                     YLKLNKTEDFAKLVDQTTLDLTYKADKGQCVILDNEDVTDFLRNNDVTQHVSYVASKE
                     PVRSFAVKKQKELAAEKGIVMDGRDIGTVVLPDADLKVYMIASVEERAERRYKDNQLR
                     GIESNFEDLKRDIEARDQYDMNREISPLRKADDAVTLDTTGKSIEEVTDEILAMVSQI
                     K"
     gene            8631..8783
                     /locus_tag="P6L01_001713"
     CDS             8631..8783
                     /locus_tag="P6L01_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030780.1"
                     /translation="MQRLTHIQNECADNDLLKLKGDFYSMMNESCLFIFGKSSCVSEL
                     CKFFKK"
BASE COUNT         3344 a         1411 c         1531 g         2705 t
ORIGIN      
        1 aatagaagta ggtgtattta atcatgaaaa agctaacacg aatgattaaa ctaataacaa
       61 taaaaaggta ggttcaaaaa agccatctca acaacagtta aaaccacaaa ccaatcaaac
      121 ctatccaacc aatgaactca aatcaccaat gtatcactct ccaataatgt aactatgatc
      181 taattaaagc atagttattg aggttttgtg atatatagta taaaattaat gagaattaaa
      241 tttaataatg taaaattcat cttcggggtc gggtgtaatt cccaaccggc agtaaataaa
      301 gcctgcgacc tgctagtatg tatcatatta gtggctgatc tagtgagatt ctagagccga
      361 cagtatagtc tggatgggag aagatggagg ttttttgttg tgcaataatc ctcctattct
      421 tacgagatga atggaaggag aaaattgaat atgcaacaaa ataaacgtct tatcacaata
      481 agtatgttga gcgcgattgc gtttgtgtta acttttatca agtttcctat accatttttg
      541 ccaccatact taactttaga ttttagtgat gtaccgtcac tactagctac atttacgttt
      601 ggaccagttg ccggtatcgt agttgcactg gttaaaaact tgctgaacta cttatttagt
      661 atgggcgatc cagttgggcc gtttgctaac tttttagcag gcgcaagttt cttattaact
      721 gcttacgcca tctataaaaa taaacgttca acaaaatctt tgattactgg attaatcatt
      781 gcaacaatcg ttatgactat cgtgttgagt attttgaact atttcgttct attacctttg
      841 tacggtatga tatttaactt agctgatatc gcaaataatc ttaaagtaat cattgtatca
      901 ggaattatac cattcaatat tattaaaggt atcgtcattt ctattgtatt tattttacta
      961 tatagaaggc ttgcgaattt cttgaaaaga atttaatcaa attaaagcaa aataatatac
     1021 acataataat aaaaagcagg tgactatcaa taaacgatag cttgcctgct ttttctatag
     1081 aacatttgtc taaaaaatca attattcaaa ttttaaagcg tccccatcaa atgattcgtc
     1141 tgcaatttta atagaatctg tagggcatcc atcaattgca tcttccatat cttcatataa
     1201 ttcctcaggt acttctgcag taccttggtt atcgtcaagg attacgaaag caataccttc
     1261 gtcgtcgtaa tcatatatat ctggcgctgc tgcaccgcat gcaccacatg caatacaagt
     1321 atccatatca acgattgtat attttgccaa tgtcttcgcc tcctttgata aaaatgctaa
     1381 aatagtaatg tgactaaaat tttagacagc atcattttta ttttcaaatt atccgtttta
     1441 cagagtgagg gttaaatttg caacacatta taaaaacagc attacaacaa acatttaact
     1501 ataaaacaaa taaaagtatt tacaatatct tagttggtaa gaaatctcac caaacctttt
     1561 ttgacgcttg tagtcaacaa cagttgtcat tatatcacag tttaccacta ttaaaatatc
     1621 cgtcttttga gctattttta gaaaaaatca atgaatttaa tgctgaaatg gaaatcatgt
     1681 tgcatcctag atatacattt gaaagcatgg ggcaaacatt tcaagcaatt caactattag
     1741 tgcaaaccat gtctaatacc aaacaacatg tttttcattt tgtaccaatc tctcaaaata
     1801 ataagataca agaaactgta aagattgtct ataactacat taaagaaaac caattacaaa
     1861 ttgactttga aaatgaacta cataacttgt ttaaagcaat aactttaaaa gggccatgtt
     1921 acttacatta ttatttgcaa ggctatgatg aaccaatgta tacgagacag caagttagtt
     1981 taatagaaaa actatctcaa cagcaattgt ttgaatacga aatgaataat ttagtgacaa
     2041 tgatgtttga attagaaagt ggagaatata ctattttatc aaaaataata atgaaaccta
     2101 cattattaaa tcaaacttat attacttata caaaattgct tgaacaattc acgatggaag
     2161 atatagcggc tcaacaacaa gttaaaatca atactatcga agatcatgta cttgaaatct
     2221 taatcaaagg ttacatgtct aattacgatg attatgttga actagaagat caactccagt
     2281 ttttgaattt ttatcaacaa catcgtggcg aacgattaaa attttacaaa gaacaatttg
     2341 acacgttatc atattttcaa ttaaaagtat taatcgttgg atttgaaaga ggtgatctga
     2401 atgttgcatg atattttacg aaacaaattt ggcttcgaga attttaaacc gggacaacag
     2461 gaaattatag aaagtataat gtctcaacaa cacactctag gtattcttcc aactggaagt
     2521 ggaaagagtt tgtgttatca aatacctacg tatttatcag gtaagccgac attaattatc
     2581 tcaccgttaa tatctttaat ggatgaccaa gttatgcagt tgaaaataaa tggagaaaaa
     2641 cgtgtaacat gtattcactc tggtatggat gaaattgaga aaaagcataa tattaaatgt
     2701 ttactacata gccgcttcat ctttctaagt ccagaatttc tcctgcaacc gtcaaatttt
     2761 aaattaatat ctatgataga ctttggcatg attgttctag atgaagcaca ttgcctatct
     2821 gaatggggat atgatttcag accacattat gctctaatag gaaaagtaac aaagcatttt
     2881 aaagaagcgg ttgtcttagc gttgacagca actgcaccac cgcatttaca agatgatttg
     2941 acggaaatgt tagcgattca attcaacgtt attaaaacta caatgaatcg cccaaacata
     3001 agctttaagc atcttaattt tcatgatgat gaagataaaa ttgagtggtt gctgccgttt
     3061 ctacaacagt cgggaccaac gattatctat gtctcatcga aaaagatgtg tctgaattta
     3121 gcgcaactta tttatgattc aggttttctt actggcattt atcatggtga tatgaattat
     3181 caagagcgac acacagttca acaacaattt ttaaataatg atattccgat tatagtcgca
     3241 acgagtgctt ttggtatggg tattaataaa aaagatattc gcacaatcat tcactttcat
     3301 ctttcaacaa gtccttctaa ctacattcaa gaaattggcc gtgcaggtcg cgatggtgaa
     3361 ctaagtcagg caattagttt atttcaaccg gacgataaat atattttaga aacgttatta
     3421 tttgcagata tgataacaga agaagatgta caaaatttcg aaataggaga atttttagca
     3481 cccgataaac aagacgtttt gacaacgttg cattcattct atagtatcgg cgccttgaaa
     3541 cagatattta agcaatcatt taaacgaaag caattaggat tctttcgcat gattggctat
     3601 tgcaaattgg atcaatgtag acggaagtat ttattagaat ttttcggtga atatccaccg
     3661 gcacaagatc gatgttgtga caatgattct aatataactg atatcgtaat tttaaataag
     3721 aagaaggtaa ttagaagtat tggatttgat gaaaagttgc aaaatttatt tctcagatag
     3781 tattacttta ctaaaagaaa attgacaagc tataattagt gtatacacaa ttgaaaaatg
     3841 attgaaataa ttttgaaaaa tatacataaa catatgtcat gtgggtatat tttatgtaaa
     3901 atcattgtaa tagaatagaa aggaagatgg ctatgtctaa taattttaaa gatgactttg
     3961 aaaaaaatcg tcaatcgata gacacaaatt cacatcaaga ccatacggaa gatgttgaaa
     4021 aagaccaatc agaattagaa catcaggata caatagagaa tacggagcaa cagtttccgc
     4081 caagaaatgc ccaaagaaga aaaagacgcc gtgatttagc aacgaatcat aataaacaag
     4141 ttcacaatga atcacaaaca tctgaagaca atgttcaaaa tgaggctggc acaatagatg
     4201 atcgtcaagt cgaatcatca cacagtactg aaagtcaaga acctagccat caagacagta
     4261 cacctcaaca tgaagaggaa tattataata agaatgcttt tgcaatggat aaatcacatc
     4321 cagaaccaat cgaagacaat gataaacacg agactattaa agatgcagaa aataacactg
     4381 agcattcaac agtttctgat aagagtaaag ctgaacaatc tcagcaacct aaaccatatt
     4441 ttgcaacagg tgctaaccaa gcaaatacat caaaagataa acatgatgat gtaactgtta
     4501 agcaagacaa agatgaatct aaagatcatc atagtggtaa aaaaggcgca gcaattggtg
     4561 ctggaacagc gggtgttgca ggtgcagctg gtgcaatggg tgtttctaaa gctaagaaac
     4621 attcaaatga cgctcaaaac aaaagtaatt ctgacaagtc gaataactcg actgaggata
     4681 aagcgtctca agataagtct aaagatcatc ataatggcaa aaaaggtgca gcgatcggtg
     4741 ctggaacagc aggtttggct ggaggcgcag caagtaaaag tgcttctgcc gcttcaaaac
     4801 cacatgcctc taataatgca agccaaaacc atgatgaaca tgacaatcat gacagagata
     4861 aagaacgtaa aaaaggtggc atggccaaag tattgttacc attaattgca gctgtactaa
     4921 ttatcggtgc attagcgata tttggaggca tggcattaaa caatcataat aatggtacaa
     4981 aagaaaataa aatcgcgaat acaaataaaa ataatgctga tgaaagtaaa gacaaagaca
     5041 catctaaaga cgcttctaaa gataaatcaa aatctacaga cagtgataaa tcaaaagagg
     5101 atcaagacaa agcgactaaa gatgaatctg ataatgatca aaacaacgct aatcaagcga
     5161 acaatcaagc acaaaataat caaaatcaac aacaagctaa tcaaaatcaa caacagcaac
     5221 aacaacgtca aggtggtggc caaagacata cagtgaatgg tcaagaaaac ttataccgta
     5281 tcgcaattca atactacggt tcaggttcac cggaaaatgt tgaaaaaatt agacgtgcca
     5341 atggtttaag tggtaacaat attagaaacg gtcaacaaat cgttattcca taatataact
     5401 atataaattg taactgaact gctatataca aacgtgcata ttataagtat ctctaaatat
     5461 tttggagaac ttaaattgcg cttataagta tgtagcggtt ttttcatttt tcaaagtttg
     5521 ttatttaaca aggtcttgtc tcgaatattg gcatatcaat taaacttttt aaatagtcat
     5581 caaaaagata aaacaccaca atcaacaaat ttaacgaggg agaataaaaa ataaatcaac
     5641 atattaaatt gtagtgttat tcaactccgt agctaacaat tctctatgca cattaaacaa
     5701 attgtcaaaa atatatcata aatcttcaag cacagactta gcgcatcaat cactgaactg
     5761 ttataatagt ttgggattaa aggaggccga aacaatgcaa aaagttgaaa gtatcataat
     5821 tggtggaggg ccatgcggat taagtgcggc tattgaacaa aaaagaaaag gtattgatac
     5881 cttaattatt gaaaagggga atgtcgttga atcaatctat aattatccta ctcaccaaac
     5941 atttttctca tcaagtgata aattaagtat tggggacgta ccgtttatcg ttgaagaaag
     6001 taaaccaaga cgtaatcaag cgctagttta ttaccgagaa gttgtaaaac atcatcaatt
     6061 aaaagtaaat gcatttgaag aagtattaac tgttaaaaaa atgaataata aatttactat
     6121 tactacgacg aaagatgttt atgaatgtcg atttttaaca atcgcgacag gctattatgg
     6181 tcagcataat acattagaag ttgaaggagc ggatttacct aaagtgttcc attattttaa
     6241 agaggcacat ccgtattttg atcaagatgt tgttattatt ggtggtaaga attcggctat
     6301 cgatgctgct ttggagttgg aaaaagctgg tgctaacgtg acggttctat atcgtggtgg
     6361 agattattcg ccttcaatta aaccgtggat acttccaaat ttcacagcat tagtaaatca
     6421 tgaaaaaatt gacatggaat ttaatgctaa tgttacccaa ataactgaag atactgtgac
     6481 ttatgaagta aatggtgaaa gtaaaacaat acacaatgat tatgtatttg cgatgattgg
     6541 ttatcatccc gattatgaat ttttaaaatc tgtaggcatt caaattaata caaatgaatt
     6601 tggaacagcg cctatgtata ataaagaaac atacgaaaca aatatcgaaa attgctatat
     6661 tgcaggtgta attgctgcag ggaacgatgc gaataccatt tttattgaaa atggtaaatt
     6721 ccacggtggc attattgctc aaaacatgct agctaagaaa caaacgccgt tagaatcata
     6781 aaaataaagg tctatgtact aaataactta gttttacaac gactgacatt catgatatgt
     6841 caaatgaggt tgatgactaa ttattgtaca tagacctttt tatgttacgt attcattata
     6901 attcaaaata tgatttgatt tcagctttat ctaaattgtt gcttaacgca actaataatt
     6961 ttaatcttgc ttttggacca ttcaaaccgt tagaaaaaat aaaaccttgt tgtgcgagtt
     7021 ggtaaccacc accatcgtat gcgtaagttg gactcacaat accattaaag gaacgtgaaa
     7081 ctagcacaat aggtatattt aaagatacta attgttgaat gccttctaat gcgcttggag
     7141 gtatgttgcc ttgtcctaac gcttcaataa ccataccatc gataccttca cgactataaa
     7201 aactaaaaat gtcacctggc atacccatat atgcttttac taatggtaca tttagtttgt
     7261 cattgacatt ttccaatgct tgttggcgat atggcatatg atggaattgc acacgatcct
     7321 ttgtcaatac acctagcgga ccatgatttg gactttgaaa tgtgtttgta ttagacgtat
     7381 gtgttttggt aacattacgc gccgtatgaa tttcatcatt aaatacaacc atcacgcctt
     7441 tatgacgggc cttttcatca gaggcaacgc gaatagcgga aatataatta tataatccgt
     7501 cagaaccaat ttcattagac gagcgcattg cgccagtaat aacaacaggt tgctcgatac
     7561 ctaatattaa atcaagtaaa aaggcagttt cttctaacgt atcggtacca tgcgtgataa
     7621 cgaaaccatc ataatattta tttgttactg cttctaaaat aatatccttt aattgtttaa
     7681 catgttggat tgtcatatga ggtgatggta cattaaaagg atttaattca tctatttgtg
     7741 catattgatt tatgacatct tgatgcattg aaatagggtt gatatcattt gttactactt
     7801 tattagattg gtcttgtgac atactaatgg tgccaccagt atgaataaca agtagatgtt
     7861 tcatatattt cctcctatat ttaatttacc taattatgat aaaatattat tcataaaacg
     7921 acaaggaagg gaaatgacgc atgaaagcca ttaatattgc attagatggt ccagctgctg
     7981 ccggaaaaag tacaattgcg aaacgtgtag ccagcgaact atcaatgatt tatgtcgata
     8041 caggagcaat gtatcgtgca ttaacataca aatatttaaa attaaacaaa actgaggact
     8101 ttgcaaaact agttgaccaa acaacattag atttaactta taaagcagat aaaggtcaat
     8161 gtgtcatttt agataacgaa gatgtaacag actttttaag aaataatgat gtgacgcaac
     8221 atgtttcata cgttgcatct aaagagccag tacgttcatt cgccgttaaa aaacaaaaag
     8281 agttagctgc agaaaaaggt atcgtaatgg atggtcgcga tatcggaact gtagtgctac
     8341 cagatgcaga tttaaaagta tatatgattg catcagttga agagcgagca gaaagaagat
     8401 ataaagataa tcaattaaga ggtatcgaat caaattttga agatttaaaa cgtgatattg
     8461 aagctcgtga tcaatatgac atgaaccgtg aaatatcacc attaagaaaa gcagatgatg
     8521 cagtgacatt agatacgaca ggcaagtcga ttgaagaagt tactgacgaa attttagcga
     8581 tggtgagtca aattaaataa tctaataagt cataactatc tatttgtaat gtgcaacgct
     8641 taacgcatat acaaaatgaa tgtgctgata atgatttact caaattaaaa ggtgattttt
     8701 attcaatgat gaatgaaagt tgccttttta tttttggtaa aagttcatgc gtcagtgaat
     8761 tgtgtaagtt tttcaaaaag taaaaagaaa taataaaggt gaattattag aattccaaaa
     8821 ataattcatt acattcataa agcattttac aaatggtaag aaaatgagtg ttacaaatct
     8881 aaatattgca aaagaacttg atttagtcac aaaaaatgtc ctatgtaata attcgagaaa
     8941 gatgcactat atacggtctt cttactattc aaatgtaaaa gttgcttatt t
//
LOCUS       DAOBBH010000102         8958 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.102, whole genome shotgun sequence.
ACCESSION   DAOBBH010000102 DAOBBH010000000
VERSION     DAOBBH010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8958
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.102"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..320)
                     /locus_tag="P6L01_001714"
     CDS             complement(<1..320)
                     /locus_tag="P6L01_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDP3030781.1"
                     /translation="MLVALNEEKERVLATTALRKTQYFCPVCGKQVILKRGLKVISHF
                     AHKHLAEQKCFNNETIKHYKSKLILAQMIQQQGCKVEIEPFLKEIKQIPDILINNKYV
                     IELQ"
     gene            complement(441..1160)
                     /gene="mecA"
                     /locus_tag="P6L01_001715"
     CDS             complement(441..1160)
                     /gene="mecA"
                     /locus_tag="P6L01_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDP3030782.1"
                     /translation="MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSM
                     MDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQEL
                     LAQTLEGEDQLEELFEQRTKEKEAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHS
                     NPITTEFEDLLYMVDGTYYYAVHFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYA
                     KIIMSHNVTAQVRRYFPETTE"
     gene            complement(1531..1926)
                     /gene="spxA"
                     /locus_tag="P6L01_001716"
     CDS             complement(1531..1926)
                     /gene="spxA"
                     /locus_tag="P6L01_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDP3030783.1"
                     /translation="MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQ
                     ILKMTEDGTDEIISTRSKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQ
                     VGYNEDEIRRFLPRKVRTFQLQEAQRMVD"
     gene            2220..3209
                     /gene="trpS"
                     /locus_tag="P6L01_001717"
     CDS             2220..3209
                     /gene="trpS"
                     /locus_tag="P6L01_001717"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDP3030784.1"
                     /translation="METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHA
                     ITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGEL
                     ERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVD
                     RFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKI
                     KSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEI
                     VKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKVSFKTVKKMEKAMGLGRKR"
     gene            complement(3251..4132)
                     /locus_tag="P6L01_001718"
     CDS             complement(3251..4132)
                     /locus_tag="P6L01_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3030785.1"
                     /translation="MQNKSKSPFKIALSRFIHNKIAMLSIIFLLIITIVSIIAPLIAP
                     FPVNQQDLLNIKGEMTAQNILGTDSGGRDNFSRLLYAGRISLSIGITSTIGMLLIGIT
                     VGVISGYFGGIVDTLLMRITEFVMLFPFLIFAIVLNAALGDKIKNPYGSAIILVLVII
                     VLSWGGIARLVRGKVLQEKENEYFLAAKSIGTPTYKIILKHLLPNILSVVIVQATLLF
                     AGMIVVESGLSFLGFGISKAIPSWGNMLSDAQEGDVISGKPWIWMPPAIMITLTILSI
                     NFVGEGLKDAFNPRGRR"
     gene            complement(4144..5106)
                     /locus_tag="P6L01_001719"
     CDS             complement(4144..5106)
                     /locus_tag="P6L01_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3030786.1"
                     /translation="MLKLILKRLGLMIPLLILISIVVFSLAIIQPGDPFSDLQNGKIK
                     QEAINAQREKLGLNDSIPHQYIRWVNHVIHGDLGESIKYKRPVIDVIEERIPNTILLG
                     AMSLIITYIISFALGITSGRYSYSLTDYTVQIFNYLMLAIPSFIAGVFAIFIFSFELQ
                     WFPFQGSVDINLKEGTFEYYMSKIYHTFLPALTLGLLSTAGYIQYLRNDIIENSKKDY
                     VLTARSKGLSMNKIYNKHILRNSLIPIITFLGADIVSILGGAVITETIFSYNGIGKLF
                     LESVIGQDYPLMMALTLFFSFLGLLGNLISDITYGFIDPRIRSN"
     gene            complement(5099..6079)
                     /locus_tag="P6L01_001720"
     CDS             complement(5099..6079)
                     /locus_tag="P6L01_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDP3030787.1"
                     /translation="MENILEVNQIKKYYKIKTGLLQKTQYVKAVDDVSFSIKKGQTFG
                     LVGESGCGKSTLGKVIIRLEDATSGSIIVNGEDITRLQGKKLRKSRQQYQMIFQDPYA
                     SLNPMQMVGDIISEPILNYKKLPKEEIKKEVLYLLKCVGLSEDAYYKYAHEFSGGQRQ
                     RVGIARALALRPSLIVADEPVSALDVSVQSQVLNLLKDLQEQFKLSYLFIAHDLSVVK
                     HISDVIGVMYLGHIVEIASDKEIYENPKHPYTKALISSIPQIDKHNNNRIILKGELPS
                     PSNPPQGCPFHTRCPIAKDKCKENIPQLKDIGNEHQVACFYVDKVGDLNA"
     gene            complement(6082..7068)
                     /locus_tag="P6L01_001721"
     CDS             complement(6082..7068)
                     /locus_tag="P6L01_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3030788.1"
                     /translation="MNNVLLEVKDLETSLKINNEWLATVENISFELSKGEVLGIVGES
                     GCGKSILSKSIIKLLPEKISKLSSGEVIFDGKRIDTLNEKQLLDIRGNDIAMIFQEPM
                     TALNPVFTIKNQLVESIKSHKKISKKEANNLAKDLLKKVGIARQDEILNSYPHQLSGG
                     MRQRVMIAMAISCSPKLLIADEPTTALDVTIQAQILDLLKELQKETQMAIMMITHDLS
                     VVAEFCDKVLVMYAGQIVEFGGIKEILHNPKHPYTQKLLSTIPKLKEGQKRLETIEGI
                     VPSIQAFHVNKCRFANRCNKKLDICNNQSPKMHVCEDVIVRCHLYKNEYKEI"
     gene            complement(7119..8834)
                     /locus_tag="P6L01_001722"
     CDS             complement(7119..8834)
                     /locus_tag="P6L01_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDP3030789.1"
                     /translation="MGKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTETSKHKG
                     GTLNVALTAPPSGVYSSLLNSTHADSVVEGYFNESLLATDKKIRPKAYIASWKDIEPA
                     KKIEFKIKKGIKWHDGNELKIDDWIYSIEVLANKDYEGAYYPSVENIQGAKDYHEGKT
                     DHISGLKKIDDYTMQVTFDKKQENYLTGFITGHLLSKKYLSDVPIKDLAKSDKIRKYP
                     IGIGPYKVKKIVPGEAVQLVKFDDYWQGKPALDKINLKVIDQAQIIKAMEKGDIDVAN
                     DATGAMAKDAKSSNAGLKVLSAPSLDYGLIGFVSHDYDKKANKTGKVRPKYEDKELRK
                     AMLYAIDREKWIKAFFNGYASEINSFVPSMHWIAANPKDLNDYKYDPEKAKKILDKLG
                     YKDRDGDGFREDPKGNKFEINFKHYSGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFG
                     KYNEDLANASKDMEVYFRSWAGGTDPDPSDLYHTDRPQNEMRTVLPKSDQYLDDALDF
                     EKVGIDEKKRKDIYVKWQKYMNDELPGLPMFQGKSITIVNDKVRNLDIEIGTDQSLYN
                     LTKEA"
BASE COUNT         2942 a         1568 c         1179 g         3269 t
ORIGIN      
        1 tactgtagct caataacata tttattatta atcaaaatat ccggaatttg ttttatttct
       61 tttaaaaatg gctctatctc tactttacat ccttgttgct gtatcatctg tgctaaaatc
      121 aatttacttt tataatgttt aatcgtttca ttattaaaac atttttgttc cgctaaatgt
      181 ttatgtgcaa aatgactaat tactttgagc ccacgcttta aaataacttg cttgccacac
      241 acaggacaaa aatattgtgt ctttctcaat gcagtagttg ctaacacacg ttccttttct
      301 tcatttaaag ctactaacat atcttcacct caaaatatta cacgtaagtt taactactat
      361 tactttttca gcttttgaaa tcaataaaaa aagagactcc aaagagtctc ctttcatagt
      421 gcgttgctta attaatatcg ttattcagtt gtctctggaa aataacgtcg aacttgagct
      481 gttacgttat gactcataat tattttagca tagtcattta aataaacttc tgttctgtct
      541 gttggatatg caaattcaag caattgactg taactatcat taatgacttc ttgatcaaca
      601 tgactatcaa aatgtacagc ataataataa gtaccatcaa ccatatataa caaatcttca
      661 aactctgtag ttattggatt gctatgatat gcataattaa taacatcttc taaatcgtta
      721 aatttcacaa tgattgttct tgtattttta cgtgctgaag acttttgacg tttagaacct
      781 tgagcttctt tttcttttgt tcgttgctcg aataattctt ctaattgatc ttcaccttct
      841 aatgtttgag ctaacaattc ttgaacttgt tcatcaaatt gatcagttgc atcatcatca
      901 gacatattca tcatatcttc atttttagat ttagaaattg tgacttcgac acctttttca
      961 aaggcatgta cttgaatcca taatggacct tctacaacaa aatcttcttc ttcgttaatt
     1021 tcatccatca ttgaccaaaa gaattcttcg ccacgtttgc gatttgtcca taaatcttca
     1081 cgactaaatc cacgggcctc gatatcgcta tatgttataa acaattttac agttgtatca
     1141 tctactcgtt ctattctcat atcatctcac tccttacagt cgatgaatag tatccacatt
     1201 gtattaaagc atcaaacaaa atacaattta tttgtttgat ttctttctct aaaatatttt
     1261 ggtaacttat aataacatat tcgttgtagc aaaactattt aaatgattta tacagttaaa
     1321 gcattaaagc actttcttaa tttaatttta ccttatcata gcaaatgttt catccaaaac
     1381 actctaatat agagctatat taacaattgc acatttgatt taaaagatac taagcaatta
     1441 ttgtaaaatt aaaaccaaaa aagcaacact atagaaatag tgttgcttga catatctttt
     1501 agtaaagtat gacacatagt taaatggtta ttagtcaacc atacgttgtg cttcttgtaa
     1561 ttggaacgta cgaacttttc taggtaagaa acgtcgaatc tcgtcctcat tataaccaac
     1621 ttgtagtcgt ttattatcta aaataattgg acgacgtaat aagccaggat tatcttgaat
     1681 gattgaatat aagtcttgta atggtagtga atcaatatca acatttaatt tttggtatgt
     1741 tttagaacgt gtagaaatga tttcatcagt accgtcttca gtcattttta atatttgctt
     1801 aatttcatca attgttaaat gttcagaaaa aatattacgc tccgtatacg gaatgtcatg
     1861 ttcttgtaac catgctttcg ctttacggca agatgtgcaa cttggtgaag taaataatgt
     1921 taccatacat ctcactctcc tatttgaatg aataaaattc attgcttaaa atttagttat
     1981 agatcaagaa aaaacatttt tttctaaaat tcttaatcgt tactatttat tataactatc
     2041 taacattaaa attaaatgag aaaaacctaa tttttcagat aagtttctac acttataaaa
     2101 aagattatta atcctttgtt agtagtaagt tatacgtata ttctaacaca tcttacattt
     2161 ttaagaaata ctgttataat gataattatt aaaatattac taagaaagta ggcatttaaa
     2221 tggagacatt attttcaggc atccaaccta gtggaattcc tactattgga aattatattg
     2281 gcgcactaaa acaatttgtt gatgtgcaaa atgactatga ttgttatttc tgtatcgtag
     2341 atcaacatgc aattacaatg ccacaagatc gtttaaaatt acgtaaacag accagacaat
     2401 tagcagcgat ttatttagct tctggtatag atccagacaa agcaacattg ttcatacaat
     2461 ctgaagtccc tgcacacgta caagcaggat ggatgttaac tacgattgct tctgttggag
     2521 aattagagcg tatgacgcaa tacaaagata aagctcagaa agcagttgaa ggtataccag
     2581 ctggtctatt aacatatcca cctttaatgg cagctgatat tgttctttac aatactaata
     2641 tcgttccagt tggagatgac caaaagcagc atatcgaatt gactcgtaac cttgtagata
     2701 gatttaatag tcgctataat gatgtgcttg tgaaacctga aattcgtatg cctaaagttg
     2761 gtggacgtgt catgagttta caagacccaa caagaaaaat gagtaagagt gatgataatg
     2821 ctaaaaactt catttcatta ttggacgagc cgaatgttgc agctaaaaaa attaaaagcg
     2881 cagtaactga ttcagatggt attattaaat ttgatcgtga caacaagcca ggtataacaa
     2941 atttaatttc aatatacgct ggattaaccg acatgccaat taaagatatt gaggcaaaat
     3001 atgaagggga aggttatggt aaatttaaag gtgaccttgc tgaaatagtt aaagcatttt
     3061 tagtagaatt ccaagaaaaa tacgaaagtt tctataactc agataaactt gatgatattt
     3121 tagatcaagg tagagataaa gcacacaaag tttcatttaa aactgtcaaa aaaatggaaa
     3181 aagcgatggg attaggacgt aagagataaa ataaagaggt actagaatta actagtgcct
     3241 cttttattat ttaacgtcta cctctaggat taaaagcatc tttaagccct tcccctacaa
     3301 agtttatact taatatagtt aatgtaatca ttatagcagg tggcatccat atccacggtt
     3361 taccacttat aacatcccct tcttgagcat cactcaacat attaccccaa gatggtattg
     3421 ctttactaat tccgaatcct aaaaagctca atcctgattc cactacaatc ataccggcaa
     3481 ataacaatgt tgcttgtacg ataactacac ttaatatatt cggcaaaaga tgtttcaaaa
     3541 taattttata tgtgggtgta ccaattgatt ttgctgccaa aaagtattca ttttcttttt
     3601 cttgaagtac tttaccacga acaagtcttg caatacctcc ccaacttaat acgataataa
     3661 ctagaacaag aattatggca gatccataag gatttttaat tttatctcca agtgcagcat
     3721 ttaatacaat tgcaaatatt aaaaatggaa ataacataac aaattcggtt attctcatta
     3781 ataatgtatc aacaatacct ccaaaataac cagaaatcac tccaactgta attccaatca
     3841 aaagcattcc tattgtagat gtaattccaa tggataatga aatacgacct gcatataaca
     3901 aacgactaaa gttatcccta ccaccagagt ctgtaccaag aatgttttgt gctgtcattt
     3961 caccttttat atttaataaa tcttgttggt tcactggaaa aggagctatt aatggcgcta
     4021 taattgatac aatagttatg attaataaaa aaataatcga taacattgca attttattat
     4081 gaataaatct agacaatgca attttaaaag gcgattttga cttattttgc attccaatcc
     4141 cccctagtta cttctaattc ttggatctat aaatccataa gtaatatcag aaatcaaatt
     4201 acccaataaa cctaaaaatg agaaaaataa cgttaatgcc atcattaatg gatagtcttg
     4261 acctattacc gattctaaaa ataatttacc gataccgtta tatgaaaaga tagtctcagt
     4321 aatcacagct ccacctaaaa tacttacaat atcagcacct aaaaatgtaa taataggtat
     4381 taaagaattt ctcaatatat gtttattata aattttattc atagataatc cttttgacct
     4441 tgccgtcaat acataatctt ttttagaatt ttcaataata tcattacgta aatattgaat
     4501 ataaccagca gtagataata atcctaaagt taatgcaggc aaaaatgtat gataaatttt
     4561 actcatataa tattcaaaag taccttcttt aaggttaata tcaacagaac cttgaaacgg
     4621 aaaccattgt aattcaaaag aaaaaataaa aattgcaaat actcccgcaa taaaagatgg
     4681 aatagctaac atcaaataat taaatatttg cacagtataa tcagtcaaac tgtaagaata
     4741 tctacctgac gttattccta aagcaaatga gataatataa gtaataatta atgacatagc
     4801 accgagtaat attgtatttg gaattctttc ctcaataaca tcaattaccg gcctcttata
     4861 tttgattgac tcccctaaat caccatgtat aacatgattg acccatctaa tatattgatg
     4921 tggtattgag tcgttgaggc ctaacttttc cctttgtgca tttattgctt cttgttttat
     4981 tttaccgttt tgtaaatctg aaaatggatc tcctggttgt atgattgcta atgaaaatac
     5041 aactattgag attaaaatta gtaacgggat cattaaccct aaccttttaa gtattaattt
     5101 aagcatttaa atcacctact ttatctacgt agaaacaagc aacttgatgt tcgttaccaa
     5161 tgtcctttaa ttgtggtata ttttctttac atttatcttt tgcaatcgga catcttgtat
     5221 gaaaaggaca accttgcggc ggattacttg gcgaaggtaa ttctcctttt aatataattc
     5281 tattgttatt atgtttatca atttgtggta ttgatgaaat taatgctttt gtatatggat
     5341 gtttgggatt ttcataaatt tctttatcag atgcaatttc aactatatga cctaaataca
     5401 taactccaat tacatcacta atatgtttca ctacacttaa atcatgcgcg ataaataaat
     5461 agcttaactt aaattgctct tgtaaatctt ttaataaatt cagtacttga gattgaacag
     5521 atacatctaa tgcacttaca ggctcatcag caacaattaa actcggacgc aaagccaatg
     5581 ctcttgcaat tcccactctt tgtctctgtc cacctgaaaa ttcatgtgca tatttataat
     5641 atgcatcttc acttaggcca acacatttta ataaatatag tacttctttt tttatttctt
     5701 cttttggcaa ttttttataa tttaaaatag gttctgaaat gatatctcca accatttgca
     5761 tcggattcaa tgatgcatac ggatcttgaa atatcatctg atattgttgt cgtgattttc
     5821 tgagtttttt accttgtaat cttgttatat cttcaccatt aacaattatt gaacctgaag
     5881 ttgcatcttc aagcctgata atcactttac ctaacgttga cttaccacaa cccgattctc
     5941 ctactaatcc aaaagtttgt ccttttttta ttgaaaacga tacgtcatca acagctttaa
     6001 cgtactgagt tttttgtaat aatccagttt taattttgta gtattttttt atttggttga
     6061 cttctaaaat attttccatt cttatatctc cttatattca tttttgtaca aatgacaacg
     6121 tacaatgacg tcttcacaaa catgcatttt aggagattga ttattacaaa tatccagttt
     6181 tttgttacat ctatttgcaa atctgcactt attaacgtga aatgcttgga ttgatggcac
     6241 aattccttct atcgtttcaa gtcgtttctg cccttcttta agttttggaa ttgttgataa
     6301 taatttttgg gtataaggat gtttcggatt gtgtagtatt tcttttatgc ctccaaattc
     6361 tacaatttga cctgcataca taactaagac tttatcgcaa aactcagcaa ctacactcaa
     6421 atcatgtgta atcatcataa ttgccatttg cgtttccttt tgcaattctt ttaataagtc
     6481 taatatttgc gcttgaatcg tgacatccaa tgctgttgta ggttcatcag caattaataa
     6541 tttaggagaa catgaaatgg ccattgcaat cattactctt tgtctcatac caccagataa
     6601 ttgatgagga tagctattta atatttcatc ttgtctagca attccaactt tttttagtaa
     6661 atcttttgct aagttatttg cttctttttt agaaattttt ttatgtgatt ttatagattc
     6721 cacaagttga ttttttatgg taaatacagg atttaaagca gtcataggtt cttgaaaaat
     6781 catagcaata tcatttcctc gaatatctag caattgcttc tcattgagcg tatcgattcg
     6841 tttaccatca aatataactt ctccactact tagtttagat atcttttctg gtaataattt
     6901 aataattgac ttacttaata tggacttacc gcaaccagat tcccctacta tacccaaaac
     6961 ttctccttta gataattcaa aagaaatatt ttcaacagtt gctaaccatt cattatttat
     7021 ttttaatgat gtttctaaat ctttaacctc taacaataca ttattcataa catcccccca
     7081 tataagtaaa cctcgacaaa atcgtcgagg ttacattact aagcttcttt agttaaatta
     7141 tataaacttt gatcagttcc aatttcaatg tctaagtttc gtactttatc gttaacaata
     7201 gttatcgatt taccttggaa cattggtaat ccaggtaact catcattcat atatttttgc
     7261 catttaacat aaatatcttt acgtttcttt tcatcaatgc ctactttttc gaagtctaat
     7321 gcatcatcta aatattgatc tgattttggt aaaactgttc tcatttcatt ttgaggtcta
     7381 tcagtgtggt ataaatctga tggatctgga tctgtacctc ctgcccatga tctgaagtac
     7441 acttccatat ctttagatgc atttgctaag tcttcattat atttaccgaa ttctactaac
     7501 ttcacatttg ttttcaagcc aactttttcc cagaaatctt ttatcgcagc agttcttggt
     7561 tcaaaagtag gatttgaacc tgaataatgt ttaaagttaa tctcaaattt attaccttta
     7621 ggatcttctc taaatccgtc accatctcta tctttataac ctaacttatc taagattttt
     7681 ttagcttttt caggatcata tttgtaatca tttaggtcct taggattggc tgctatccaa
     7741 tgcatagatg gtacaaaact attgatttca ctagcgtaac cattgaaaaa cgctttaatc
     7801 catttttctc tatcaattgc ataaagcatt gctttacgta attctttgtc ttcatatttt
     7861 ggtctcactt taccagtttt attagctttt ttatcgtaat catgagatac gaatcctatt
     7921 aaaccgtagt ctaagcttgg cgcagataat accttgagac cagcattaga tgacttagca
     7981 tcttttgcca ttgcaccggt agcatcattc gcaacatcaa tatcgccttt ttccattgcc
     8041 ttaataattt gagcttgatc aataactttt aaattgattt tgtctagtgc aggcttacct
     8101 tgccaataat catcaaattt aacgagttga acagcctcac ctggaacgat tttcttaact
     8161 ttatacggtc caataccaat aggatatttt cggattttat ctgatttcgc taaatcttta
     8221 attggtacat ctgataaata ttttttactt aataaatgtc cagtaataaa tcctgttaag
     8281 taattttctt gttttttatc aaatgtaacc tgcatagtgt agtcatctat tttcttcaat
     8341 ccgctaatat gatcagtttt tccttcatga taatctttcg caccttggat attttctaca
     8401 cttggataat aagcaccttc gtagtccttg ttagctaaga cttcaattga ataaatccaa
     8461 tcatcaattt tcaattcatt accatcatgc catttaatac cttttttaat tttaaattct
     8521 attttcttag ccggctcgat gtccttccat gaagcaatat atgccttagg acgtattttt
     8581 ttatcagttg ctaataagct ttcgttaaaa tatccctcaa ctacagaatc tgcatgtgta
     8641 ctatttaata acgaagaata aacaccactt ggcggtgctg ttaatgctac atttaaggta
     8701 ccacctttat gttttgaggt ttccgtttta gtagcatctt ttactccttc atctttatta
     8761 ctgctttttc cgcaagcact caacactaaa acgacaataa gaagtattga aatatattta
     8821 attagcttcc ccatacatat gcctcctact ttcaatctaa ttttttcttt ttagtttact
     8881 catagaaaaa attattacaa gtttattttt agaatattca gttatttatg catttaatca
     8941 atttcagtaa tttaaaaa
//
LOCUS       DAOBBH010000103         8725 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.103, whole genome shotgun sequence.
ACCESSION   DAOBBH010000103 DAOBBH010000000
VERSION     DAOBBH010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8725
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.103"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            77..1153
                     /locus_tag="P6L01_001723"
     CDS             77..1153
                     /locus_tag="P6L01_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="HDP3030790.1"
                     /translation="MLFKEAQAFIENMYKECHYETQIINKRLHDIELEIKETGTYTHT
                     EEELIYGAKMAWRNSNRCIGRLFWDSLNVIDARDVTDEASFLSSITYHITQATNEGKL
                     KPYITIYAPKDGPKIFNNQLIRYAGYDNCGDPAEKEVTRLANHLGWKGKGTNFDVLPL
                     IYQLPNESVKFYEYPTSLIKEVPIEHNHYPKLRKLNLKWYAVPIISNMDLKIGGIVYP
                     TAPFNGWYMVTEIGVRNFIDDYRYNLLEKVADAFEFDTLKNNSFNKDRALVELNYAVY
                     HSFKKEGVSIVDHLTAAKQFELFERNEAQQGRQVTGKWSWLAPPLSPTLTSNYHHGYD
                     NTVKDPNFFYKKKESNANQCPFHH"
     gene            1173..1967
                     /locus_tag="P6L01_001724"
     CDS             1173..1967
                     /locus_tag="P6L01_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDP3030791.1"
                     /translation="MQLYYLGPKGTFSYLACRQYFSENEATFQPKSNLFEVIKAVADD
                     DTSIGVVPIENSIEGTINIVADALAQQDVFAHGEIRLDINFALYGNGTDSISDIKKVY
                     SIAPAISQTTNYIHQHQFDYDYVDSTIQSLTKIENGVAAIAPLGSGEAYGFTPIDTHI
                     EDYPHNVTRFLVIKNQQQFDQNATSLMFLITPMHDKPGLLASVLNTFALFNINLSWIE
                     SRPLKTQLGMYRFFVQADSAITTDIKKVIAILETLDFKVEMIGAFN"
     gene            2039..2146
                     /locus_tag="P6L01_001725"
     CDS             2039..2146
                     /locus_tag="P6L01_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030792.1"
                     /translation="MVDFLLFVLFLHDAAVYVSLPLVQAIANVTFYFSF"
     gene            complement(2157..3719)
                     /locus_tag="P6L01_001726"
     CDS             complement(2157..3719)
                     /locus_tag="P6L01_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDP3030793.1"
                     /translation="MAYFNQHQSMISKRYLTFFSKSKKKKPFSAGQLIGLILGPLLFL
                     LTLLFFHPQDLPWKGVYVLAITLWIATWWITEAIPIAATSLLPIVLLPLGHILTPEQV
                     SSEYGNDIIFLFLGGFILAIAMERWNLHTRVALTIINLIGASTSKILLGFMVATGFLS
                     MFVSNTAAVMIMIPIGLAIIKEAHDLQEANTNQTSIQKFEKSLVLAIGYAGTIGGLGT
                     LIGTPPLIILKGQYMQHFGHEISFAKWMIVGIPTVIVLLGITWLYLRYVAFRHDLKYL
                     PGGQTLIKQKLDELGKMKYEEKVVQTIFVLASLLWITREFLLKKWEVTSSVADGTIAI
                     FISILLFIIPAKNTEKHRRIIDWEVAKELPWGVLILFGGGLALAKGISESGLAKWLGE
                     QLKSLNGVSPILIVIVITIFVLFLTEVTSNTATATMILPILATLSVAVGVHPLLLMAP
                     AAMAANCAYMLPVGTPPNAIIFGSGKISIKQMASVGFWVNLISAIIIILVVYYVMPIV
                     LGIDINQPLPLK"
     gene            complement(3924..5021)
                     /locus_tag="P6L01_001727"
     CDS             complement(3924..5021)
                     /locus_tag="P6L01_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HDP3030794.1"
                     /translation="MYINMKDYGLTGINKTKDTRAIQRALNRGRCKPTTVYIPKGTYD
                     ICKPLTIYGNTTLLLDNETILRRCHSGPLLKNGHRFGFYRGYNGHSHIHIKGGKFDMN
                     GVSYPYNNTAMCIGHAEDIQLIGVTIKNVVSGHAIDACGINGLYIKSCSFEGFIDYSG
                     ERFYSEAIQLDIQVPGAFPKFGTTDGTITKNVVIEDCYFGPSELPEMGSWNRAIGSHA
                     SRHNRYYENIHIRNNIFEDIQGYALTPLKYKDAFIINNKFINCEGGIRYLGVRDGKNA
                     ADVMTGKDLGSQAGINMNIIGNEFKGSMSKDAIHVRNYNNVKHKDVLIVGNTFNNSTQ
                     SIHLEDIDTVFLSPVEAGIQVTTINIDEIKK"
     gene            5390..5950
                     /locus_tag="P6L01_001728"
     CDS             5390..5950
                     /locus_tag="P6L01_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDP3030795.1"
                     /translation="MTHRALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNY
                     YQDHIFFLMDLHYLHDIHHPESKLFPPHNIVDTSGRELYGKVGKLYETIKAQPNVHFI
                     DKTRYDSFFGTPLDSLLRERSINQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVA
                     SFNQKGHEWALAHFKNSLGAEVEQHV"
     gene            6003..6932
                     /locus_tag="P6L01_001729"
     CDS             6003..6932
                     /locus_tag="P6L01_001729"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDP3030796.1"
                     /translation="MAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVS
                     AETQFALDTFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIAN
                     FETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQ
                     QDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQ
                     VNAVDLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGE
                     AFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK"
     gene            complement(7125..7298)
                     /locus_tag="P6L01_001730"
     CDS             complement(7125..7298)
                     /locus_tag="P6L01_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030797.1"
                     /translation="MIVVAVNFKWSFELYMLKVENRAMNRVHNNIYNFNHNEKGRKKT
                     KIYTQHRKYFKKM"
     gene            7350..>8725
                     /locus_tag="P6L01_001731"
     CDS             7350..>8725
                     /locus_tag="P6L01_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDP3030798.1"
                     /translation="MNIIEQKFYDSKAFFNTQQTKDISFRKDQLKKLSKAIKSYESDI
                     LEALYTDLGKNKVEAYATEIGITLKSIKNARKELKNWTKTKNVDTPLYLFPTKSYIKK
                     EPYGTVLIIAPFNYPFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDA
                     NYIEVIEGGIEETQTLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVI
                     VDETANIKVASERICFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNI
                     QQSPDYGRIVNLKHYHRLTSLLNSAQMNIVFGGHSDEDERYIEPTLLDHVTSDSAIMQ
                     EEIFGPILPILTYQSLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAIND
                     TLMHLANPKLPFGGVGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKF
                     KYIKAFFK"
BASE COUNT         2977 a         1494 c         1355 g         2899 t
ORIGIN      
        1 acaatttaat gacatataca taatttttaa gagtatttta ataatgtaga ctataatata
       61 aagcgaggtg ttgttaatgt tatttaaaga ggctcaagct ttcatagaaa acatgtataa
      121 agagtgtcat tatgaaacgc aaattatcaa taaacgttta catgacattg aactagaaat
      181 aaaagaaact gggacatata cacatacaga agaagaactt atttatggtg ctaaaatggc
      241 ttggcgtaat tcaaatcgtt gcattggtcg tttattttgg gattcgttaa atgtcattga
      301 tgcaagagat gttactgacg aagcatcgtt cttatcatca attacttatc atattacaca
      361 ggctacaaat gaaggtaaat taaagccgta tattactata tatgctccaa aggatggacc
      421 taaaattttc aacaatcaat taattcgcta tgctggctat gacaattgtg gtgatcctgc
      481 tgaaaaagaa gttacacgct tagcaaatca cttaggttgg aaaggaaaag gtactaattt
      541 tgacgtgtta ccactgattt accaattacc taatgagtca gttaaatttt acgaatatcc
      601 tacttcatta attaaagaag tacctattga acataatcat tatccaaaat taagaaaatt
      661 gaacttaaaa tggtatgcag tccctatcat ttccaatatg gacttaaaaa tcggtggcat
      721 tgtatatcca actgcaccct ttaacggttg gtatatggta actgaaattg gcgtacgtaa
      781 ctttattgat gattaccgtt acaatttact agaaaaagtt gcagatgcgt ttgaatttga
      841 tacacttaaa aataattcat ttaataaaga tcgagcactt gttgaattga actatgctgt
      901 gtatcattcc tttaaaaaag aaggcgtatc aattgtcgat catttgaccg ctgcaaagca
      961 attcgaacta ttcgaacgta acgaagcaca acaaggtcgt caagttaccg gaaaatggtc
     1021 ttggctagca ccgccattat ctccaacatt gacgtcaaat tatcatcacg gatatgacaa
     1081 tacagtaaaa gatccaaact ttttctataa aaagaaagaa tcaaatgcta accagtgccc
     1141 tttccatcat taagttagta gaggtgtagc atatgcaatt atattattta ggccctaaag
     1201 gtacattttc atatttggct tgtcgtcagt atttttctga aaatgaagca acttttcaac
     1261 caaagtcaaa tttatttgaa gtcatcaagg cagttgcaga tgatgacaca tcgattggtg
     1321 ttgtccctat tgaaaattct atagaaggaa caatcaacat tgtggcagat gcacttgcac
     1381 agcaagatgt ctttgcacac ggtgaaatac gtttagatat taactttgca ttatatggta
     1441 atggaacaga ttccatttct gatattaaaa aagtgtattc tattgcacca gcaatcagtc
     1501 aaacaacaaa ttacatacat caacatcaat ttgactatga ttatgtcgac agtacaattc
     1561 aaagtttgac taaaattgaa aatggcgttg cagcaattgc acctttaggt agtggcgaag
     1621 cttatggttt tacacctatt gatacacata ttgaagacta tccacataac gtgacacgtt
     1681 ttttagttat aaaaaatcag cagcaatttg atcaaaatgc gacatcattg atgtttttaa
     1741 ttacaccaat gcatgataaa cctgggctgt tagcaagtgt gcttaataca tttgctttgt
     1801 ttaatattaa tttatcctgg attgagtctc gtcctttaaa aacacaatta ggtatgtatc
     1861 gatttttcgt tcaggctgat tctgcaatta caaccgatat aaagaaagtc attgctattt
     1921 tagaaacctt agatttcaaa gtagaaatga ttggtgcttt taattaaact ttattttcta
     1981 ggttctacaa tttaaataaa ataaagagtc aaccaaggat taaaatagga atccatcctt
     2041 ggttgacttt cttttgtttg tattgttttt acacgatgct gcggtatacg tttcattacc
     2101 actcgttcaa gcaattgcta atgtaacctt ttatttttcg ttctaatctg caattactat
     2161 ttcaatggca gtggttgatt tatatcaata cctaaaacta taggcattac ataatacacg
     2221 actaaaataa taattattgc actgattaag tttacccaga atcctactga tgccatttgt
     2281 ttgatagata ttttacctga accaaagata attgcattcg gtggtgtccc tactggtaac
     2341 atgtatgcac agttagccgc catagctgca ggtgccataa gtagtaatgg atgcactcca
     2401 acagcaacag acaacgttgc taaaatcggt aaaatcatcg ttgcagttgc agtattagat
     2461 gtcacttcag ttaaaaataa gacaaagatt gttatgacaa ttacaataag aatcggacta
     2521 acaccattta atgatttcaa ctgttcgcct aaccattttg ctaaaccact ttcagaaata
     2581 cctttcgcta atgctaaacc gccaccaaat aaaattaata caccccaagg gagctctttt
     2641 gcaacttccc agtcaatgat acggcgatgt ttttcagtat ttttagctgg aataataaat
     2701 aataatattg atataaaaat agcaatcgta ccatctgcaa cagatgacgt aacttcccat
     2761 tttttcagaa gaaactctct tgtaatccat aataagctgg caagtacaaa gatagtttgt
     2821 actacctttt cttcatactt cattttgcca agctcgtcta acttttgttt aattaacgtc
     2881 tgaccaccag gtaaatattt caaatcatgt ctaaacgcaa catatcttaa atagagccaa
     2941 gtaataccta acaaaacaat gaccgttgga atccctacaa tcatccattt agcaaaacta
     3001 atttcatgtc caaaatgttg catgtattgt ccttttaaaa taattaatgg cggggttccg
     3061 attaatgtac ccaagccacc aatcgtacct gcatagccaa ttgctaaaac tagagatttt
     3121 tcaaactttt gaatacttgt ttgattcgta ttggcttctt gtaaatcatg tgcttcctta
     3181 ataattgcta aaccaatcgg aatcataatc attacagctg cagtgttcga tacaaacata
     3241 gataagaatc ctgttgccac catgaatcca agtaatattt ttgaagtgct tgcaccaatt
     3301 aaattaataa tagtcaaagc tactctcgta tgtaaattcc atctttccat tgcaattgcc
     3361 aaaataaatc cacctaaaaa caaaaagata atatcattgc catattcgga tgatacttgt
     3421 tctggtgtaa gtatgtgacc taatggtaat aacacaattg gtaataagct cgttgctgca
     3481 ataggaattg cttcagtaat ccaccaagtc gcaatccata aagtaatcgc taagacatat
     3541 acgcctttcc aaggtaagtc ttgtggatga aagaacaata atgttaatag gaaaagtaat
     3601 ggacctaata ttagtccaat aagttgtccc gcactaaacg gtttcttttt ctttgatttt
     3661 gaaaagaatg ttaaatacct tttcgatatc attgattgat gttgattgaa ataagccata
     3721 aatttccccc tcatgatgat tatgacaagc tacttcaact cactatatta gagtatagaa
     3781 agttgtctat cttgtattat cattaccgtc aaaatacccc ttttatcaga taattgggtt
     3841 tattatagca tatttatacc gttttatgaa tgcgctttca aaaattatct actattctta
     3901 atcctaatgt catgcgaaac tttttacttt tttatttcat ctatattgat tgtagtaact
     3961 tgaatacccg cttcaacagg acttaaaaac actgtatcaa tatcttctaa atgaattgat
     4021 tgagtcgaat tattgaatgt attcccaacg attaatacat ctttatgttt aacattatta
     4081 taattacgta catgtatcgc atctttagac attgatcctt taaattcatt tccaattata
     4141 ttcatattta tgcctgcttg ggaacctaag tcttttcctg tcatcacatc tgctgcattt
     4201 ttaccatctc taactcctaa atatctaata ccaccctcac agttaataaa cttattatta
     4261 ataatgaaag catctttata cttcaaggga gttaatgcat aaccttgtat atcttcaaat
     4321 atattatttc taatatgaat attctcatag taacgattat gtctacttgc atgtgagcca
     4381 atagcacgat tccaacttcc catttcgggc aattctgaag gtccaaaata acaatcttcg
     4441 ataacgacat ttttcgttat cgtaccatct gtcgttccga attttggaaa agcaccaggt
     4501 acttgaatgt ctaattgtat tgcttcagaa taaaagcgtt cgccactata gtctatgaat
     4561 ccttcaaatg aacagctttt aatatagagt ccgttaatcc cacaagcatc aattgcatga
     4621 ccactcacta cattcttaat ggtcacacct attaattgaa tatcttcagc atgcccaatg
     4681 cacatagctg tattgttata aggatacgat acaccattca tatcaaactt gccgccttta
     4741 atatgaatat gactgtgtcc attataacca cgataaaaac caaagcgatg accatttttt
     4801 aataaaggac cagaatgaca tcggcgtaaa atagtttcat tatctaacaa aagtgttgta
     4861 ttgccatata tcgttaatgg tttgcaaata tcatacgtcc ctttcggtat ataaactgtc
     4921 gttggtttac atcttccacg atttaacgca cgttgtattg ctcgagtatc tttagttttg
     4981 tttatgcctg ttaacccata atctttcata ttgatataca taaatcgtca tcctttgtct
     5041 attttctaat tactcatata ttaaccaatt attcaaatta taatcgtgtt aggttgattt
     5101 agcagatagt cataaatctc gtgatgccat ctattagcat ttttatattc aaaacatcaa
     5161 cacttgatat tagttctaag tttgtcacat ttagtattct attacagcta ttttaaaact
     5221 atcgcaaaaa cttcttatat tatttgttga ttgcgtgatt tgctcttcca aaaaagttga
     5281 ttaatctata tacttaatct atatttatat attaaccatt agggttaaaa attactctag
     5341 catttatgaa tagatgggag tttattttat tatataggag agatgttgaa tgacacatcg
     5401 cgcactatta gttgttgact attcatatga ctttatcgca gatgacggct tactaacatg
     5461 cggtaaacct ggacaaaata ttgaagattt tattgtttct cgtatcaatg actttaatta
     5521 ttatcaagac catatattct ttttaatgga tttacattat ttacatgaca ttcatcatcc
     5581 tgaaagtaaa ttattcccac cacacaatat cgtagataca agtggtagag aattatacgg
     5641 taaagtaggt aaattatacg aaacaattaa agcgcaacct aatgtacatt tcattgataa
     5701 aacgcgctat gattcgttct ttggtacccc gcttgatagt ttattgagag aaagaagtat
     5761 taatcaagtc gaaatcgttg gtgtatgtac cgatatttgc gtgttacata cagcaatttc
     5821 tgcatacaac ttaggttata aaatttcagt acctgctgag ggagtggctt catttaatca
     5881 aaaagggcat gaatgggcac ttgcacattt caaaaactca ttaggtgcag aggtagaaca
     5941 acacgtttaa atcgtgctaa aataattata aagaatacaa tttacaaggg agatatttga
     6001 caatggctaa aacatatatt ttcggacata agaatccaga cactgatgca atttcatctg
     6061 cgattattat ggcagaattt gaacaactta gaggtaattc aggagccaaa gcataccgtt
     6121 taggtgatgt gagtgcagaa actcaattcg cgttagatac atttaatgta cctgctccgg
     6181 aattattaac ggatgattta gatggtcaag atgttatctt agttgatcat aacgaattcc
     6241 aacaaagttc tgatacgatt gcctctgcta caattaagca tgtaattgat catcacagaa
     6301 ttgcaaattt cgaaactgct ggccctttat gttatcgtgc tgaaccagtt ggttgtacag
     6361 ctacaatttt atacaaaatg tttagagaac gtggctttga aattaaacct gaaattgccg
     6421 gtttaatgtt atcagcaatt atctcagata gcttactttt caaatcacca acatgtacac
     6481 aacaagacgt taaagcagct gaagaattaa aagatattgc taaagttgat attcaaaagt
     6541 acggcttaga tatgttaaaa gcaggtgctt caacaactga taaatcagtt gaattcttat
     6601 taaacatgga tgctaaatca tttactatgg gtgactatgt gactcgtatt gcacaagtta
     6661 atgctgttga ccttgacgaa gtgttaaatc gtaaagaaga tttagaaaaa gaaatgttag
     6721 ctgtaagtgc acaagaaaaa tatgacttat ttgtacttgt tgttactgac atcattaata
     6781 gtgattctaa aattttagtt gtaggtgctg aaaaagataa agtcggcgaa gcattcaatg
     6841 ttcaattaga agatgacatg gccttcttat ctggtgttgt ttctcgaaaa aaacaaatcg
     6901 tacctcaaat cactgaagca ttaacaaaat aatactatat tactgtctaa tcatagacat
     6961 gttgtattta actaacagtt cattaaagta gaatttattt cactttcaat gaactgtttt
     7021 ttatttacgt ttgactaatt tacaaccttt tcaatagtag ttttcattcc tttagctatc
     7081 ctaacccaca gattagtgat ttctatacaa ttcctctttt gtctttacat tttcttaaaa
     7141 tatttgcgat gttgagtata aatttttgtt ttcttcctac ctttttcgtt atgattaaag
     7201 ttataaatat tattatgtac acgattcatc gctctatttt caactttcaa catatataat
     7261 tcgaaagacc atttaaaatt aacggccaca acaatcaaat caattaatca ctttttccaa
     7321 aataatcata taaggaggtt cttttcatta tgaatatcat tgagcaaaaa ttttatgaca
     7381 gtaaagcttt tttcaataca caacaaacta aagatattag ttttagaaaa gatcaattaa
     7441 agaagttaag caaagctatt aaatcatacg agagcgatat tttagaagca ctatatacag
     7501 atttaggaaa aaataaagtc gaagcttatg ctactgaaat tggcataact ttgaaaagta
     7561 tcaaaaatgc ccgtaaggaa cttaaaaact ggactaaaac aaaaaatgta gacacacctt
     7621 tatatttatt tccaacaaaa agctatatca aaaaagaacc ttatggaaca gttttgatca
     7681 ttgcaccatt taactatcct tttcaactag tattcgaacc tttaatcggt gctattgcag
     7741 caggtaatac agcaattatt aaaccatctg agttgacacc aaatgttgca cgagtgatta
     7801 aacgattaat caatgaaaca tttgatgcaa attacattga agttattgag ggaggaattg
     7861 aagaaacgca aacgttaatt cacttacctt ttgactatgt cttctttaca ggaagtgaaa
     7921 atgtaggcaa aatcgtttat caagctgcca gcgaaaattt agttcctgtg acattagaaa
     7981 tgggtggtaa atctccagtc atcgttgatg aaacagcgaa tattaaagtt gctagtgagc
     8041 gcatttgttt tgggaaattc actaatgccg gccaaacatg tgttgcacca gattacattt
     8101 tagtacacga atctgtaaaa gatgatttaa tcacagccct atcaaaaacg ttgcgtgaat
     8161 tttatggtca aaatatacaa caaagtccag attatggccg cattgtaaac cttaaacatt
     8221 atcatcgtct gacttcatta cttaacagtg cacaaatgaa tattgtattt ggtggtcata
     8281 gtgatgagga tgaacgttat atagaaccaa cattgttaga tcacgttaca agtgattcag
     8341 caattatgca agaagaaatt tttggtccta tcttaccgat tttaacgtat cagtcattgg
     8401 atgaagcaat agcctttatt caccaaagac caaaaccttt gagtttatat ttatttagcg
     8461 aagatgaaaa tgctacacaa cgtgtaataa acgagttatc atttggcggc ggcgctatta
     8521 atgatacatt gatgcaccta gcgaatccta aattaccatt tggtggtgtt ggcgcctcag
     8581 gtatgggacg ctatcatggt aaatattcat tcgacacttt tacacatgaa aaaagctaca
     8641 ttttcaaatc tacacgatta gaatcaggtg tccatttacc accatataaa ggtaaattta
     8701 aatacatcaa agctttcttt aaaaa
//
LOCUS       DAOBBH010000104         8668 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.104, whole genome shotgun sequence.
ACCESSION   DAOBBH010000104 DAOBBH010000000
VERSION     DAOBBH010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8668
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.104"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..67)
                     /gene="graF"
                     /locus_tag="P6L01_001732"
     CDS             complement(<1..67)
                     /gene="graF"
                     /locus_tag="P6L01_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance-associated protein GraF"
                     /protein_id="HDP3030799.1"
                     /translation="MSNENQNKKAAEKAKEVEEKLK"
     gene            219..1514
                     /locus_tag="P6L01_001733"
     CDS             219..1514
                     /locus_tag="P6L01_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030800.1"
                     /translation="MSFLRKHAEIIFSYLIGIVSLFTGLIILINLPLIKQLNGGKKVD
                     THVHNVWEFLNAFFSEIIKVMSRFIGNFPIVSAIVIIIFGILVMLIGHTLLRTIKYDY
                     DISIFFLVIGIMYFIITLILMTQVYGFFAVIFIIPFTIHIGYIVYKDELNQENVKNHF
                     MWIIVSYGISYLITQIALYGRIDANEIESIDILSVNAFFIIMWLLGQMAIWNFLFLRR
                     ALPLTKQELGEEEPELSRTSKGNVTNQTKIHLKQLQDKTTEYARKTRRSVDLDKIRAK
                     RDKFKKKVNDIIDIQEDDIPDWMRKPKWVKPMYVELFCGVVIFLFTFLEFNNRNALFV
                     SGDWKLSQTQYVIEWVTLLILLFIIIAYIATTLTFHLKGKFYYLQLFMGSILFFKLLT
                     EFINIMIHGLLLSVFITPTLLLMLLAIIISYSLQLRERP"
     gene            1939..3111
                     /locus_tag="P6L01_001734"
     CDS             1939..3111
                     /locus_tag="P6L01_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDP3030801.1"
                     /translation="MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDK
                     SQYVATAYVGRQHKGLGWVLTYDKAQEINTAFFVKLFNTALAERDYYFNIDGTNAFRL
                     FNAEGDGVGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDS
                     EYSGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRGQYAKEHHV
                     LNLFSYTGAFSVIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDF
                     YKYAARHGHSYDTIVIDPPSFARNKKRTFSVQKDYDKLINGALNILSSEGTLLLCTNA
                     SVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVFVNIRH"
     gene            3165..3707
                     /locus_tag="P6L01_001735"
     CDS             3165..3707
                     /locus_tag="P6L01_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HDP3030802.1"
                     /translation="MGFKNNLTSNLTNKIGNSVFKIENVDGKGAMPTTIQELRERRQR
                     AEAIVKRKSLMSSTMSVVPIPGLDFGVDLKLMKDIIEDVNKIYGLDHKQVNSLGDDVK
                     ERIMSAAAIQGSQFIGKRISSAFLKIVIRDVAKRTAAKQTKWFPVVGQAVSASISYYF
                     MNKIGKDHIQKCENVIKNVM"
     gene            3861..4127
                     /locus_tag="P6L01_001736"
     CDS             3861..4127
                     /locus_tag="P6L01_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDP3030803.1"
                     /translation="MEQNSYVIIDETGIHARPATMLVQTASKFDSDIQLEYNGKKVNL
                     KSIMGVMSLGVGKDAEITIYADGSDESDAIQAISDVLSKEGLTK"
     gene            4130..5848
                     /gene="ptsP"
                     /locus_tag="P6L01_001737"
     CDS             4130..5848
                     /gene="ptsP"
                     /locus_tag="P6L01_001737"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDP3030804.1"
                     /translation="MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAK
                     FNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANA
                     ATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
                     VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGD
                     MIIVDGLNGDVIVNPTEDELIAYQDKRECYFADKKELQKLRDADTVTVDGVHAELAAN
                     IGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMDGKRVVVR
                     TLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMF
                     PMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDF
                     FSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMA
                     GDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIEL
                     VNNYVK"
     gene            complement(6085..6318)
                     /gene="nrdH"
                     /locus_tag="P6L01_001738"
     CDS             complement(6085..6318)
                     /gene="nrdH"
                     /locus_tag="P6L01_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDP3030805.1"
                     /translation="MSEIIVYTQNDCPPCTFVKNYLNEHQIDFEERNINNQQYRNEMI
                     DFDAFSTPFILLNGNPMYHVDLDEINKVLNIQD"
     gene            6516..7877
                     /locus_tag="P6L01_001739"
     CDS             6516..7877
                     /locus_tag="P6L01_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDP3030806.1"
                     /translation="MDTVEISRFLTAMTLAVHIIFATIGVGMPLMFAIAEFLGIRKND
                     LQYIAMAKRWAKAYTITVAVGVVTGTIIGLQLSLIWPTFMEMGGHVIALPLFMETFAF
                     FFEAIFLSIYLYTWDRFKNKWTHFLISIPVIIGGSFSAFFITSVNSFMNTPAGFELKN
                     GKMVNVQPIEAMFNPSFIVRSFHVITTAGMTMAFVIASIAAFKLLRNRQPKDTVYHKK
                     ALKMSMIVGFFSTLLSMLAGDLSAKFLHKFQPEKLAAYEWHFDTSSHAKLLLFGVLDE
                     KTQQVKGAIELPGLLSFLADNSVKTKVQGLNDFPKSLHPPMIVHYFFDLMVTMGILCF
                     VISGVYVLTLMFKKLRKFSTHKWMLYGILLTGPASMLAIEFGWFLTEMGRQPWIVRGY
                     MRVAEAATQAGGITFVTILFGILYIILMYTCAYVLIRMFKNKPAYEDVNRLAKKQGGE
                     IEK"
     gene            7874..>8668
                     /locus_tag="P6L01_001740"
     CDS             7874..>8668
                     /locus_tag="P6L01_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDP3030807.1"
                     /translation="MIYAFIGITVLWLFLFCYIIIASIDFGAGFFALHSKLTGDEKKI
                     NHLISRYLNPVWEVTNVFFVFFFVGFVGFFPESIKYLGTVLLIPGSIALIMISLRNSF
                     YAFENYGQDTKLAWMIMYGVSGLLIPASLSTALTITEGGYINVRNNVIDLDWVQLLLS
                     PFAWSVVFLAIISVLYISSGFLTYYAKKANDEPAYNLTRQWHIFLGPPMIIICLFVFL
                     SLRIQNSEHFYSAVFDYWWMFAISFLFFALASLLTFFKKKHGLAFVF"
BASE COUNT         2972 a         1157 c         1547 g         2992 t
ORIGIN      
        1 cttttaattt ttcttcaact tctttagctt tttctgctgc ttttttattt tgattttcat
       61 tagacatgat taattcctcc caaattggat aattatttat atataaatct tacccggttg
      121 tactttcgtt aaacttttct aagtctatag cactatttat tcatttatct aaagacaaca
      181 acattagatt aatatataat gattttgagg tgaacataat gtcttttctt aggaaacacg
      241 ccgaaattat ttttagctat ttaatcggta tcgtttcact cttcactggt ctcattattt
      301 taattaactt gccattaatt aaacaattaa atggtggtaa aaaagttgat acacatgttc
      361 ataatgtgtg ggaatttctg aatgcatttt tcagtgaaat tattaaagta atgagtcgat
      421 ttataggtaa tttccctata gttagtgcaa ttgtgataat tatattcggt attttagtta
      481 tgttgattgg tcatacatta cttagaacta ttaagtatga ctatgatatt tctatctttt
      541 tcttagttat cggtatcatg tactttatta ttactcttat attaatgact caagtttatg
      601 gattctttgc agtgattttc attattccat ttacaattca tataggatat atcgtctata
      661 aagatgaatt gaatcaggaa aatgtaaaaa atcatttcat gtggataatt gtgagttatg
      721 gtataagtta cttaattaca caaattgcat tgtatggcag aattgatgct aatgaaatag
      781 agtcaattga tatcttaagt gtcaatgctt tctttataat tatgtggtta cttggtcaaa
      841 tggctatttg gaatttcttg ttcttgcgcc gagctttacc tttaacaaag caagaattag
      901 gtgaagagga gccagaatta tcaagaacaa gtaaagggaa tgtcacgaat caaactaaaa
      961 ttcacttgaa acaactccaa gataagacta cagaatatgc acgtaagaca agaagaagtg
     1021 tcgatttaga taaaattaga gctaaaagag ataaattcaa aaagaaagtt aatgatatta
     1081 tcgatattca agaagacgat attcctgatt ggatgagaaa accgaaatgg gttaaaccaa
     1141 tgtatgtcga actattttgt ggtgtcgtca tctttttatt cacattttta gaatttaata
     1201 atcgtaatgc attatttgta tctggtgatt ggaaattatc acagacacaa tatgttattg
     1261 aatgggttac attattaatt ctgttattca ttattatcgc atatatcgct acaacgttaa
     1321 ctttccactt gaaaggtaag ttttattatt tacaactatt tatggggagc attttattct
     1381 ttaaattgtt aacggaattt ataaatataa tgattcatgg actactactt tcagtgttca
     1441 ttacgccaac attactatta atgttattgg caatcatcat ttcttattcg ttacaattac
     1501 gagagcgacc ataattaaaa gcattataaa agtactatct attaaacatt ttgatgtgta
     1561 cgctataagt tagatatatc tctaacttat ttagatacag gtcaatgaag tttatggata
     1621 gtactttttt tgtaactaga tttgattgat tcaggtgatg tgaattaagt attgaaaatt
     1681 gtatacaaag ttttagtgca aataaaatag ttgaaaagtt atccatttgt aaaatcaaga
     1741 aaactagtaa atagttgaag cgacatatgg aatttgcgaa acgatatata gtatttcctt
     1801 tgtagaaatt taacatatat cattcgaatt actaatttgt taaaatcaac agtaagatta
     1861 gaagtagatg atattgaaat ttggcaaaca atttaatcta tataaaacta caacgaaaca
     1921 cagaaaggaa gttgtcagat gaaaatagca actctgaaca aaggcaaaga aacaaaatat
     1981 tttaatggat atcctttaat tgaagaagag gatatctatt cacaagatca tttaaaagaa
     2041 ggagatattt ttcaaattgt gactgataaa tcacaatatg ttgcaacggc ttatgttggt
     2101 cgccaacata aaggattagg ttgggttcta acatacgata aagctcaaga aatcaacaca
     2161 gctttctttg tgaaattgtt taatactgca ttagcagaac gtgattatta ttttaatata
     2221 gatggaacaa atgcttttag attatttaat gctgaaggtg atggtgttgg gggattaaca
     2281 atcgacaatt acgatggtca tttgttgatt caatggtact caaaaggtat ttataaattt
     2341 aaatatgcca ttcttgaagc ggttagaaaa gtatttgatt ataaatctat ttacgaaaaa
     2401 gtaagattta aagacagcga atatagtggt ggttttgttg aaggcgatgc acctgagttt
     2461 ccaattgtta tcgaagaaaa cttcacattt tataatgtag accttgaaga tggtttgatg
     2521 acaggtatct ttttagatca aaaagaagtg cgcaagaaat taaggggtca atatgccaaa
     2581 gaacaccatg ttttaaactt atttagttat acaggtgctt tttctgtaat agcagcaagt
     2641 gaggcatctt caacaacaag tgtagatttg gctaatcgtt ctcgtagttt aactgaagaa
     2701 aattttggat taaatgctat tgatcctaaa tcccaatata tttatgtcat ggacactttt
     2761 gatttctata aatatgctgc acgacatgga catagttatg acacgatcgt gattgatcca
     2821 cctagctttg cacgcaacaa aaaacgtaca ttttcagtgc aaaaagatta tgacaaatta
     2881 attaatggcg ccttaaatat tttatcatct gaaggaacat tattgttatg tacaaatgca
     2941 agtgtatatc cattaaagca atttaaaaat actattaaaa agacgcttga agagagtggc
     3001 gttgattatg aattaactga agttatgggt ttaccaaaag attttaaaac gcatccacat
     3061 tataagccat ctaaatattt aaaagctgtt tttgtaaata ttagacatta atttgataaa
     3121 aaggggtata gtaacttatt gagaaaaaga agggtgataa tattatggga ttcaaaaaca
     3181 atttaacatc aaatttaaca aataaaattg gtaattcagt ctttaaaata gaaaatgttg
     3241 acggaaaagg tgcaatgcca acgacgattc aagaattgag agaaagacga caacgtgctg
     3301 aagcaattgt aaagagaaag tctttaatgt catcaacaat gagcgttgtt ccaattccgg
     3361 gtttagattt tggtgttgat ttaaaattaa tgaaagatat tatcgaagat gtaaataaaa
     3421 tatacggatt agatcataag caagttaata gccttgggga tgatgtgaaa gaaagaatta
     3481 tgtctgcagc agcaattcaa ggtagtcaat ttattggtaa aagaatttca agtgcatttt
     3541 taaaaatagt tattagagat gtagctaaac gtactgctgc aaaacaaaca aaatggtttc
     3601 ctgttgtagg acaagctgtg tctgcatcta ttagttacta ttttatgaat aaaattggaa
     3661 aagatcacat tcaaaaatgc gaaaatgtta ttaaaaatgt catgtaggtg ctataatagt
     3721 tttgcaattt gcaaatttta ctgaaaccgg ttttaaacga attgaattta aagcatggtt
     3781 ttggtaaagt taatgtataa aactaagtta gtattgtaat aatattgaag attctaacta
     3841 tacgaaggag aaatgtaatt atggaacaaa attcatatgt aatcatcgac gagactggta
     3901 ttcacgctag accagcaaca atgttagtac aaacagcttc aaaattcgat tctgatattc
     3961 aattagaata taacggtaag aaagtaaact taaaatcaat catgggtgtt atgagccttg
     4021 gtgttggtaa agatgctgaa attacaattt atgctgacgg tagtgatgaa tctgacgcca
     4081 ttcaagcaat cagtgacgtc ttatcaaaag aaggattgac taaataatca tgtctaaatt
     4141 aattaaaggt attgctgcat ctgatggtgt cgcaattgct aaagcttatt tattagttga
     4201 gccagactta acattcgaca aaaatgaaaa agtcactgat gttgaaggag aagttgcaaa
     4261 gttcaatagc gctatcgaag cttctaaagt tgagttaact aaaattagaa ataatgcaga
     4321 ggttcaacta ggtgctgata aagctgctat ctttgatgca catttattag ttttagatga
     4381 ccctgaatta attcaaccaa tccaagataa gattaaaaat gaaaacgcta atgctgctac
     4441 agcattaacg gatgtaacaa cacaatttgt tacaattttt gaatctatgg ataacgaata
     4501 catgaaagaa cgtgcggctg atattcgcga cgtttctaaa cgtgtgttat cacatatttt
     4561 aggtgtagaa ttaccgaatc cgagtatgat tgatgaaagc gttgttattg tagggaatga
     4621 cttaacgcca tctgatactg ctcaattaaa taaagaattc gtacaaggtt ttgctacaaa
     4681 cattggcgga agaacaagtc actctgcaat tatgagtcgt tctttagaaa ttccagcaat
     4741 tgttggtaca aaatcaatta ctcaagaagt taaacaaggc gacatgatta tcgtagatgg
     4801 attaaatggt gatgtaatcg ttaatccaac tgaagatgag ttaatcgctt atcaagataa
     4861 acgtgagtgt tattttgctg acaagaaaga attacaaaaa ctacgtgatg ctgatactgt
     4921 tacagttgat ggtgttcacg cagagcttgc tgcaaatatt ggtacaccta atgatttgcc
     4981 aggtgttatt gaaaatggtg cacaaggtat cggcttatat agaactgagt ttttatatat
     5041 gggtcgtgac caaatgccta cagaagaaga acaatttgaa gcttataaag aagtattaga
     5101 agcaatggac ggtaaacgtg ttgttgtacg tactttagat ataggtggag ataaagaatt
     5161 atcatactta aacttgcctg aagaaatgaa tccattctta ggttaccgtg cgattcgttt
     5221 atgccttgcg caacaagata ttttcagacc acagctacgt gcattattac gtgcatcagt
     5281 ttatggtaag ttaaatatca tgttcccaat ggttgcaaca attaacgaat ttagagaagc
     5341 aaaagctata ttattagaag aaaaagaaaa ccttaaaaat gaaggtcatg acatttcgga
     5401 tgatatagaa ttaggaatca tggtagagat acctgcaaca gcagcattag ctgatgtctt
     5461 tgctaaagaa gtagatttct tcagtatcgg tacaaatgat ttaattcaat acacattagc
     5521 tgctgaccgt atgtcagagc gtgtatcata tctataccaa ccatataacc cttcaatctt
     5581 acgtttagtt aaacaagtta ttgaagcgtc acataaagaa ggtaaatgga caggtatgtg
     5641 tggtgaaatg gctggagatg aaacagctat tccattattg cttggtttag gtttagatga
     5701 gttctctatg agtgcaacgt ctattctgaa agcaagaaga caaattaatg gtttaagtaa
     5761 aaatgaaatg actgaacttg ctaaccgtgc agtcgactgt gcaacgcaag aagaagttat
     5821 tgaattagtt aacaactacg taaaataatt aattctttcg aaagacagaa ttataaataa
     5881 acccgaagta ttcattgtga gtaattatga atacttcggg ttctttttta tgatttatgt
     5941 taatttcaat ggaatgttgt tgctaactaa ttaaacaact atgtgtcatt aacaaaatgt
     6001 tgttctttaa ttaatgttag ttaatttaaa aatcgatatt taatttatga tagctataaa
     6061 tgatgaagtt gatttagatg aaaattaatc ttggatattt aatactttgt tgatttcatc
     6121 aagatcaaca tggtacattg gattgccatt caacaaaata aacggagttg aaaaagcatc
     6181 aaaatctatc atttcgtttc gatattgttg attgttgata tttctctctt caaaatcaat
     6241 ttgatgctca tttagataat tttttacaaa tgtacaaggt ggacaatcat tctgcgtata
     6301 aacgattatt tctgacatgt atcatgctcc ttcgtgcttt attctaatgt aatatacaca
     6361 attatacatt gaaattcaaa tttgtgaaca cattgtgaac tgacataaat tatacacaat
     6421 ttcatagcgc ataatgtttt aaaaatacac attatagcaa ttataattaa gttcattgtg
     6481 ttgtcatcaa aaaaagaaaa aggtgatgtt tttaaatgga tacagttgaa atcagtcggt
     6541 ttttgacagc tatgacttta gcagttcata tcatttttgc aacgattggt gttggtatgc
     6601 ctttaatgtt cgcaattgca gaatttttag gtattcgcaa aaatgatctt caatatatag
     6661 ctatggccaa aagatgggct aaagcttata caattactgt agcagtggga gttgttacag
     6721 gtacaattat aggacttcaa ttatcattga tttggcctac atttatggaa atgggtggac
     6781 acgttattgc acttccatta tttatggaaa catttgcgtt cttctttgaa gctattttct
     6841 taagtatata tttatatact tgggatcgtt ttaaaaataa atggacacat ttcttaatta
     6901 gtataccagt aattattggt ggctctttct cagccttctt cattacttca gtgaattcat
     6961 ttatgaatac gcctgcaggt tttgagttga agaatggaaa gatggtcaat gttcaaccta
     7021 tagaagcgat gtttaaccca tcgtttatag ttcgatcatt tcacgtaatt actacagccg
     7081 gtatgacgat ggcatttgtt attgcatcaa tagcagcttt taaattattg cgtaatcgtc
     7141 aacctaaaga tactgtctac cataagaaag ctttgaaaat gtctatgatt gttggattct
     7201 tttcaacttt actttctatg ttggcagggg atttatctgc aaaatttttg cataaattcc
     7261 aacctgaaaa attagcagct tatgaatggc atttcgatac atcttcccat gctaaattat
     7321 tattatttgg tgtgttagat gaaaagactc agcaagttaa aggtgcgatt gaattacctg
     7381 gactattgag tttcttagca gataatagtg tcaaaactaa agtgcaaggg ttaaatgatt
     7441 ttccaaaaag tttacatccg cctatgattg tccattattt ctttgattta atggtaacga
     7501 tgggaatttt atgttttgtc atttcaggtg tttatgtttt aactttaatg tttaaaaagc
     7561 ttagaaagtt ttctactcat aaatggatgc tttacggaat attattaaca ggaccagctt
     7621 caatgctagc tatagaattt ggatggttct taacagagat gggtagacag ccttggattg
     7681 ttcgtggtta tatgcgcgtg gcagaagcag caacacaagc aggcggaata accttcgtta
     7741 caattttatt tggcatattg tacatcattt taatgtatac atgtgcatac gtattaattc
     7801 gtatgtttaa aaataaaccg gcgtatgaag atgtaaatcg tttagccaag aagcaaggag
     7861 gagaaataga aaaatgattt atgcatttat aggtataaca gtgttatggt tgtttttatt
     7921 ttgctatatc attattgctt ctatagattt tggggcaggt ttcttcgcat tgcattcaaa
     7981 gttaactggt gatgaaaaga aaattaatca cttaatttca cgttatttga acccagtttg
     8041 ggaagttacg aatgtattct ttgttttctt cttcgtagga ttcgtaggtt tctttccaga
     8101 gtcaatcaaa tatttaggca cggtattgtt aataccaggt tcaatagcac tgattatgat
     8161 atcattgaga aacagttttt atgcatttga aaattatggt caagatacaa aattagcatg
     8221 gatgatcatg tatggggtaa gtggattatt aattccagct tcattatcta ctgctttaac
     8281 tattacagaa ggtggctata ttaatgttcg aaacaatgtt atcgatctag attgggtgca
     8341 gttactatta agcccatttg cttggtctgt agtattcttg gcaattattt cagttttata
     8401 tatttcatca ggatttttga catattatgc taaaaaagca aatgacgaac cagcatataa
     8461 tttaacaaga caatggcaca tatttttagg gccaccgatg attatcattt gtttattcgt
     8521 atttctatca ttacgcattc aaaattctga acatttttat tcagctgttt ttgactactg
     8581 gtggatgttt gctataagtt tcttattctt tgcattagct tcattgttaa cattctttaa
     8641 gaaaaaacat ggtttggctt ttgtattt
//
LOCUS       DAOBBH010000105         8459 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.105, whole genome shotgun sequence.
ACCESSION   DAOBBH010000105 DAOBBH010000000
VERSION     DAOBBH010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8459
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.105"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..696
                     /locus_tag="P6L01_001741"
     CDS             <1..696
                     /locus_tag="P6L01_001741"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDP3030808.1"
                     /translation="WAFENYIKSDEYNGPDMTDFGHRALSDPEFNEQYKEQMKQFKQR
                     ILEDDTFAKQFGDLGNVYGKQWRDWVDKDGNHFDQLKTVIEQIKHNPDSRRHIVSAWN
                     PTEIDTMALPPCHTMFQFYVQDGKLSCQLYQRSADIFLGVPFNIASYALLTHLIAKEC
                     GLEVGEFVHTFGDAHIYSNHIDAIQTQLARESFNPPTLKINSDKSIFDINYEDLEIVD
                     YESHPAIKAPIAV"
     gene            896..1375
                     /locus_tag="P6L01_001742"
     CDS             896..1375
                     /locus_tag="P6L01_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDP3030809.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            1390..2229
                     /gene="fakB2"
                     /locus_tag="P6L01_001743"
     CDS             1390..2229
                     /gene="fakB2"
                     /locus_tag="P6L01_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HDP3030810.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            2315..2848
                     /gene="msrA"
                     /locus_tag="P6L01_001744"
     CDS             2315..2848
                     /gene="msrA"
                     /locus_tag="P6L01_001744"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDP3030811.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNKTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            2841..3269
                     /gene="msrB"
                     /locus_tag="P6L01_001745"
     CDS             2841..3269
                     /gene="msrB"
                     /locus_tag="P6L01_001745"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDP3030812.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMVRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            3281..3781
                     /locus_tag="P6L01_001746"
     CDS             3281..3781
                     /locus_tag="P6L01_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDP3030813.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEYVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEFINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            3781..3999
                     /locus_tag="P6L01_001747"
     CDS             3781..3999
                     /locus_tag="P6L01_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDP3030814.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWKF"
     gene            4191..5681
                     /locus_tag="P6L01_001748"
     CDS             4191..5681
                     /locus_tag="P6L01_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HDP3030815.1"
                     /translation="MDDKQHTSSSDDERAEIATSNQDQETNSSKRVHLKRWQFISILI
                     GTTLITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQDSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     IHVKSVEYKKKGKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLD
                     EAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDSLKEAKDMDISILVNEGSASASEVF
                     TGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPD
                     VTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNESTQFDQALENQV
                     KAFQQANKLEVTGEFNKETNNKFTELLVEKANKHDDVLDKLINILK"
     gene            6081..6590
                     /locus_tag="P6L01_001749"
     CDS             6081..6590
                     /locus_tag="P6L01_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDP3030816.1"
                     /translation="MIRLGKMSDLDQILNLVEEAKELMKEHDNEQWDDQYPLLEHFEE
                     DIAKDYLYVLEENDKIYGFIVVDQDQAEWYDDIDWPVNREGAFVIHRLTGSKEYKGAA
                     TELFNYVIDVVKARGAEVILTDTFALNKPAQGLFAKFGFHKVGEQLMEYPPYDKGEPF
                     YAYYKNLKE"
     gene            6602..7672
                     /locus_tag="P6L01_001750"
     CDS             6602..7672
                     /locus_tag="P6L01_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDP3030817.1"
                     /translation="MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIERE
                     MIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGG
                     FVSVPVVIAAKSLNIPTIIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADF
                     IGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQ
                     VIHLTGKGLKDAQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPM
                     LLVPLGLDQSRGDQIDNANHFADKGYAKTIDEEQLTAQILLQELNEMEQERTRIINNM
                     KSYEQSYTKEALFDKMIKDALN"
     gene            7689..8303
                     /locus_tag="P6L01_001751"
     CDS             7689..8303
                     /locus_tag="P6L01_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDP3030818.1"
                     /translation="MSQWKRISLLIVFALVFGIIAFFHESRLGKWIDNEVYEFIYSSE
                     SFITTSIMLGATKVGEVWAMLCISLLLVAYLMLKRHKIEALFFALTMALSGILNPALK
                     NIFDRERPTLLRLIDITGFSFPSGHAMGSTAYFGSGIYLLNRLNQGNSKGILIGLCAA
                     MILLISISRVYLGVHYPTDIIAGIIGGVFCIILSTLLLRNKLIN"
BASE COUNT         3144 a         1165 c         1536 g         2614 t
ORIGIN      
        1 tgggcttttg aaaattatat aaaatcagac gagtataacg gtccagatat gacagatttc
       61 gggcatcgtg cattgagtga tcctgaattt aacgaacaat ataaagaaca aatgaaacaa
      121 tttaagcaac gtattcttga agatgatact tttgcgaagc aattcggtga tttaggaaat
      181 gtttatggta aacaatggcg agattgggtt gataaagatg gtaatcattt tgatcaactt
      241 aaaacagtaa ttgaacaaat taagcataat ccagattcaa gacgacacat cgtatctgca
      301 tggaatccaa cagaaattga tacaatggca cttccgcctt gtcataccat gttccagttt
      361 tatgtccaag atggtaagtt aagttgccag ttataccaac gtagcgcaga tatcttttta
      421 ggtgtgccat ttaatatcgc aagctacgct ttattgacac accttattgc caaagaatgt
      481 ggacttgaag taggagaatt tgtgcataca tttggagatg cacatattta ttcaaatcat
      541 attgatgcga ttcaaacaca attagcacgt gaaagcttca atcctccaac attaaaaatt
      601 aacagtgaca agtctatttt cgacataaat tatgaagatt tggaaattgt tgactatgaa
      661 tcacatccag caataaaagc tccaatagca gtgtagtcat tgcatagtta gctaaccata
      721 tagacatcaa aatgacatca tagtattttc aagtgcaaaa aagtactttt ttgtgttaaa
      781 cgttttcata aattatgcaa aatcattatt tctatcacac tttatgataa aaattgtgtt
      841 aaattaaaga taacttagta ataaaaaatg aaatgataga agaaggagga taattatgac
      901 tttatccatt ctagttgcac atgacttgca acgagtaatt ggttttgaaa atcaattacc
      961 ttggcaccta ccaaatgatt tgaaacatgt taaaaaatta tcaacaggtc atactttagt
     1021 aatgggtcgt aagacatttg aatcgattgg taaaccacta ccgaatcgtc gaaatgttgt
     1081 acttacttca gatacaagtt tcaacgtaga gggcgttgat gtaattcact ctattgaaga
     1141 tatttaccaa ctaccgggcc atgttttcat atttggaggg caaacattat ttgaagaaat
     1201 gattgataaa gtggacgaca tgtatattac tgttattgaa ggtaaattcc gtggtgatac
     1261 gttctttcca ccttatacat ttgaagactg ggaagttgcc tcttcagttg aaggtaaact
     1321 agatgagaaa aatacaattc cacatacctt tctacattta attcgtaaaa aataaggggg
     1381 aaaacgacca tgacaaaaca gattatagta acagactcaa catccgattt atctaaagaa
     1441 tacttagaag caaacaacat tcatgtaatt cctttaagtt taactattga aggagcttca
     1501 tacgttgacc aagtagatat tacatcagaa gaatttatta atcatattga aaatgatgaa
     1561 gatgtaaaga caagtcagcc agccataggt gaatttatat ctgcttatga agaactagga
     1621 aaagatggct ctgaaatcat aagtattcat ctttcttcag gattaagtgg tacatataac
     1681 actgcttacc aagcaagtca aatggtagat gctaatgtaa ctgttattga ttcaaaatct
     1741 atttcttttg gtttagggta tcaaatacaa cacctagtag agcttgtaaa agaaggtgtc
     1801 tcaacttctg aaatagttaa aaagttaaat catttaagag aaaacattaa attatttgta
     1861 gttatagggc aattgaatca attaattaaa ggtggcagaa ttagtaaaac aaaaggtttg
     1921 attggtaatc ttatgaaaat taaaccaatt ggtacacttg atgatggtcg cttagagctt
     1981 gtgcacaatg cgagaactca aaattctagt atccaatact tgaaaaagga aattgctgaa
     2041 tttataggag atcatgaaat caaatccatt ggtgtcgcac atgctaacgt cattgaatat
     2101 gttgataaat tgaagaaagt ttttaacgaa gcttttcatg tgaataatta cgatataaat
     2161 gtaactacac cagttatttc tgcacatact ggtcaaggtg cgattggcct cgtagtcctt
     2221 aagaagtaaa tttaatcttt tcagtgttaa ttacttccat ttcaatcctt tatagactaa
     2281 atttataatt agatagatag aggaggtaat tcatatgaca aaagaatatg caacattagc
     2341 aggaggatgt ttctggtgca tggttaaacc atttacatca tatccaggca tcaagtcagt
     2401 cgtatctggt tatagtggcg gtcatgttga taaccctact tatgaacagg tatgtacgaa
     2461 taaaaccggc catgtcgaag cagtacaaat tacgtttgat ccagaggtta cttcctttga
     2521 aaatatatta gacatatatt tcaaaacatt tgacccaact gatgatcaag ggcaattttt
     2581 cgatagaggc gaaagctatc aaccagtcat tttctatcat gatgagcatc agaaaaaggc
     2641 tgctgagttt aaaaagcaac aattaaatga acaaggtatt ttcaagaaac cagtgattac
     2701 acctattaaa ccatataaaa atttctatcc agctgaagac taccatcaag attattataa
     2761 aaagaacccg gtacattatt accaatatca acgtggttca ggtagaaaag cgtttataga
     2821 atcacattgg gggaatcaaa atgcttaaaa aagataaaag tgaactaaca gacatagaat
     2881 atattgttac acaagaaaat ggcactgaac caccatttat gaatgaatat tggaatcatt
     2941 ttgctaaagg gatttatgta gataagattt ctggtaaacc tttatttaca tctgaagaaa
     3001 agtttcattc tgaatgtgga tggcctagct tttctaaagc gcttgatgac gatgaaatta
     3061 tagaattagt cgacaaatca tttggtatgg taagaactga agtgcgttca gaagaatcaa
     3121 atagtcattt aggacacgtc tttaatgatg gacctaaaga aagtggcggc ttaagatact
     3181 gtatcaattc cgctgcaatt caatttattc catatgaaaa attagaagaa ttgggttatg
     3241 gcgatttaat atcacatttt gataagtagg agtggaaagt atgtttaaaa aattattcgg
     3301 aaaaggcaaa gaagttcaga aagatatcgc aatttatgca ccattaactg gagaatacgt
     3361 gaaaattgaa gatattccag atcctgtatt cgcacaaaaa atgatgggcg aaggttttgg
     3421 tattaatcca actgaaggag aagttgtgtc tccaattgca ggacgtgttg acaatgtctt
     3481 tccaactaag catgcgattg ggttaaaagc agataacgga ttagaattat tagttcatat
     3541 cggtttagac acagttcaat tagacggtga aggatttgaa gtgttagtgt ctagtggtga
     3601 cgaagttaac gtcggagatc cattagtaag attcaacctt gaatttatta ataataacgc
     3661 taaatctgtt atttcaccaa ttataattac taacactgat caagcagctt caattaatat
     3721 ttatgatgaa aatgctgtga ttaaaggtga aacaaaagtg attgatgtga caatgaacta
     3781 atgaaaaact attcgtttta tcaatttgtc atgacagttc gtggtcgaca cgacgataaa
     3841 ggtcgtctag cagaagagat atttgacgat cttgctttcc caaaacacga tgatgatttt
     3901 aacatactgt ctgattatat tgagacacat ggtgatttca cattgccaat gtctgtattt
     3961 gatgatttat atgaagaata tacggaatgg aaattttaat ataattttta aaaataatag
     4021 ttagaaccag ggtgatgcaa ttcgttatcc tggttttaat ttaaaataaa ctaagttgtg
     4081 actaaaaatt aatcaattat agtgaaatat ggtgcgctat cttgcataaa ttgatatgat
     4141 taactacaca gaatttaaaa gtacataata cataataagg aagtgataca atggatgata
     4201 agcaacacac atcttcatcc gatgatgaac gcgctgaaat tgcaacaagc aatcaagacc
     4261 aagaaactaa ttcatcgaaa cgcgttcact taaaacgttg gcaattcata tcaatattaa
     4321 ttggcacaac cctaataaca gctgtcatca cagttgttgc atatattttt ataaatcaaa
     4381 aaataagtgg tttaaacaaa actgatcaag caaacttaaa taaaattgaa aatgtgtata
     4441 aaatcttaaa tagtgattat tacaaaaaac aggactctga caagttaagt aaagctgcaa
     4501 ttgatggtat ggtcaaagaa ttaaaagatc cttattctga atatttaaca aaagaacaaa
     4561 cgaaatcctt taatgaaggt gtttcaggtg attttgtagg tattggtgca gaaatgcaaa
     4621 agaaaaatga tcaaattatg gttactagcc ctatgaaggg ttctccagca gaacgtgctg
     4681 gcatccgtcc taaagatgtc attactaaag taaatggaaa atcaattaaa ggaaaagcat
     4741 tagacgaagt tgtcaaagac gttcgtggta aagaaaacac tgaagtaact ttaactgttc
     4801 aacgaggcag tgaagaaaaa gacgttaaga ttaaacgtga aaaaattcat gttaaaagtg
     4861 ttgagtataa gaaaaaaggt aaggttggag ttattactat taataaattc cagaatgata
     4921 catcaggtga attgaaagat gcagttctta aagctcacaa agatggtttg aaaaagattg
     4981 ttttagattt aagaaataat cctggtggac tactagatga agctgttaaa atggcaaata
     5041 tttttatcga taaaggaaaa actgttgtta aactagaaaa aggtaaagat actgaagcaa
     5101 ttcaaacttc taatgactcg ttaaaagaag cgaaagacat ggatatatcc atcttagtga
     5161 atgaaggttc agctagtgct tctgaagtgt ttactggtgc gctaaaggac tataataaag
     5221 ctaaagttta tgggtcaaaa acattcggca aaggtgtcgt acaaactaca agagagttta
     5281 aggatggttc attgttaaaa tatactgaaa tgaaatggtt aacgccagat ggtcattata
     5341 ttcacggtaa gggcattaaa ccagacgtta ctattgacac acctaaatat caatctttaa
     5401 atgtcattcc taatacgaaa acatttaaag ttggagacga tgataaaaat attaaaacta
     5461 ttaaaattgg tttatcagct ttaggttata aagttgataa tgaatcaacg caatttgatc
     5521 aagctttaga aaatcaagtt aaagctttcc aacaagcgaa taaactcgag gtaactggtg
     5581 aatttaataa agaaacgaat aataaattta ctgagttatt agttgaaaaa gctaataaac
     5641 atgatgatgt tctcgataag ttgattaata ttttaaaata agcgatacac actactaaaa
     5701 ttgtattatt attatgttaa tgacacgctt cctaaatttg caaagatagc aatttaggag
     5761 gcgtgtttat ttttattgac gtctaaccct aaaagatata aattagacat ttacaaatga
     5821 tgtaaataac gcaatttcta tcatcgctgt taacaattca tgctttaata tactatgagc
     5881 atatactttt taaatagtat tattcactag ttttaacaat cgattaattg gtatatgata
     5941 cttttattgg ttattttcat cccatagtgt gataattact atttttcatt cataataaag
     6001 gtttaaagca tgttaatagt gtgtaagatt aacatgtact gaaaaacatg tttaacataa
     6061 cgatataagg agtgacgtac atgatccgtc taggtaaaat gtcagattta gatcaaatct
     6121 taaatctagt agaagaagca aaagaattaa tgaaagaaca cgacaacgag caatgggacg
     6181 atcagtaccc acttttagaa cattttgaag aagatattgc taaagattat ttgtacgtat
     6241 tagaggaaaa tgacaaaatt tatggcttta ttgttgtcga ccaagaccaa gcagaatggt
     6301 atgatgacat tgactggcca gtaaatagag aaggcgcctt tgttattcat cgattaactg
     6361 gttcgaaaga atataaagga gctgctacag aattattcaa ttatgttatt gatgtagtta
     6421 aagcacgtgg tgcagaagtt attttaacgg acacctttgc gttaaacaaa cctgcacaag
     6481 gtttatttgc gaaatttgga tttcataagg tcggtgaaca attaatggaa tatccgccat
     6541 atgataaagg tgaaccattt tatgcatatt ataaaaattt aaaagaatag aggtaatatt
     6601 aatgacgaaa atcgcattta ccggaggggg aacagttgga cacgtatcag taaatttaag
     6661 tttaattcca actgcattat cacaaggtta tgaagcgctt tatattggtt ctaaaaatgg
     6721 tattgaaaga gaaatgattg aatcacaact accagaaatt aagtattatc ctatttcgag
     6781 tggtaaatta agaagatata tttctttaga aaatgccaaa gacgtattta aagtattgaa
     6841 aggtattctt gatgctcgta aagttttgaa aaaagaaaaa cctgatctat tattttcaaa
     6901 aggtggattt gtatctgtgc ctgttgttat tgcagccaaa tcattaaata taccaactat
     6961 tattcatgaa tctgacttaa caccaggatt agcgaataag atagcactta aatttgccaa
     7021 gaaaatatat acaacatttg aagaaacgct aaactactta cctaaagaga aagctgattt
     7081 tattggagca acaattcgag aagatttaaa aaatggtaat gcacataatg gttatcaatt
     7141 aacaggcttt aatgaaaata aaaaagtttt acttgttatg ggtggaagct taggaagtaa
     7201 aaaattaaat agcattattc gcgaaaactt agatgcatta ttacaacaat atcaagtgat
     7261 acatttaact ggtaaaggat taaaagatgc tcaagttaaa aaatcaggat atatacaata
     7321 tgaatttgtt aaagaggatt taacagattt attagcaatt acggatacag taataagtag
     7381 agctggatca aatgcgattt atgagttctt aacattacgt ataccaatgt tattagtacc
     7441 attaggttta gatcaatccc gaggcgacca aattgacaat gcaaatcatt ttgctgataa
     7501 aggttatgct aaaacgattg atgaagaaca attaacagca caaattttat tacaagaact
     7561 aaatgaaatg gaacaggaaa gaactcgaat tatcaataat atgaaatcgt atgaacaaag
     7621 ttatacgaaa gaagctttat ttgataagat gattaaagac gcattgaatt aatggggggt
     7681 aatgctttat gagtcaatgg aaacgtatct ctttgctcat cgtttttgca ttggtttttg
     7741 gaattatcgc gtttttccac gaatcaagac tagggaaatg gattgataat gaagtttatg
     7801 aatttatata ttcatcagag agctttatta cgacatctat catgcttggg gctactaaag
     7861 taggtgaagt ctgggcaatg ttatgtattt cattacttct tgtggcatat ctcatgttaa
     7921 agcgccacaa aattgaagca ttattttttg cattaacaat ggcactatct ggaattttga
     7981 atccagcatt aaaaaatata ttcgatagag aaagacctac attgctgcgt ttaattgata
     8041 taacaggatt tagttttcct agcggtcatg ctatgggatc aactgcatat tttggaagtg
     8101 gtatctatct attaaatcga ttaaatcaag gtaattcaaa aggtattctt atagggttat
     8161 gtgcagctat gattttattg atttccatat cacgtgtata tctaggtgta cattatccaa
     8221 cagatattat tgccggcatt attggtggcg tattttgcat tattttatca acgttattac
     8281 ttagaaataa attaataaat taaatagtaa aaaaacaaaa gcagtaaacc taaagtgtcg
     8341 taagggttta ctgcttttat aaaacgttgt tataacgtat attgtctttt acgggcatat
     8401 aaaaggggaa tatttgaaaa tgaccaatcc aacaagaacg aaacgttgtg ggggggatg
//
LOCUS       DAOBBH010000106         8427 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.106, whole genome shotgun sequence.
ACCESSION   DAOBBH010000106 DAOBBH010000000
VERSION     DAOBBH010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8427
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.106"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            9..1409
                     /locus_tag="P6L01_001752"
     CDS             9..1409
                     /locus_tag="P6L01_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDP3030819.1"
                     /translation="MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYI
                     GANFFSPVENADIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAF
                     STLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGS
                     GLEIGTLSGYIAASIMIAVLTFFLTDEQMASFGWRIPFLLGLFLGLFDLYLRRKLEES
                     PVFENDVATQPERDNINFLQIIRFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVI
                     KLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHS
                     QSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPL
                     VATWLVTKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYY
                     AEHPKEALWWVKERKN"
     gene            1953..3329
                     /locus_tag="P6L01_001753"
     CDS             1953..3329
                     /locus_tag="P6L01_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDP3030820.1"
                     /translation="MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESL
                     KKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVI
                     GDTGLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
                     LMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCHKISSYKVA
                     MFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETS
                     FISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMMFSGYVSEQCINNDE
                     WFVTNDNGYVKEQYLYLTGRQQDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNE
                     RFGQIGVLLYSGDVTLTHKNVKQFLKKKVKRYEIPSMIHHVEKMYYTASGKIAREKMM
                     SMYLRGEL"
     gene            3331..4470
                     /locus_tag="P6L01_001754"
     CDS             3331..4470
                     /locus_tag="P6L01_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDP3030821.1"
                     /translation="MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEV
                     ISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQA
                     GAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
                     KMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDN
                     FGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSN
                     FPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGAL
                     ASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF"
     gene            4445..4810
                     /locus_tag="P6L01_001755"
     CDS             4445..4810
                     /locus_tag="P6L01_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraC"
                     /protein_id="HDP3030822.1"
                     /translation="MQHYLLDSNQRLNVSFSKDSVAAYYQCCNQPYRKEVPPLMCASL
                     WPKFDLFKKYANSELILTKSAINQTQKIEVDTIYVGHLEDIECRQTRNITRYTMALTL
                     TKNDQHVITVTQTFIKAMK"
     gene            4813..5082
                     /locus_tag="P6L01_001756"
     CDS             4813..5082
                     /locus_tag="P6L01_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030823.1"
                     /translation="MKFNEIWINEYLALVNDDNPIHNEIVPGQLVSQMMLMAMSLETN
                     QCQINYVKPILINENIEFIEQHEHEIIAINDDGEIKIKISLSTKK"
     gene            complement(5283..5450)
                     /locus_tag="P6L01_001757"
     CDS             complement(5283..5450)
                     /locus_tag="P6L01_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraX"
                     /protein_id="HDP3030824.1"
                     /translation="MIIYRQYHHEGAPVYEIITKTFQHVSIKCDDSFSDTEIFKLLSL
                     LQDDIDHMKVS"
     gene            complement(5957..6787)
                     /gene="thiD"
                     /locus_tag="P6L01_001758"
     CDS             complement(5957..6787)
                     /gene="thiD"
                     /locus_tag="P6L01_001758"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDP3030825.1"
                     /translation="MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTM
                     DKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYF
                     VVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKK
                     AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAA
                     ATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
                     "
     gene            6971..7627
                     /locus_tag="P6L01_001759"
     CDS             6971..7627
                     /locus_tag="P6L01_001759"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDP3030826.1"
                     /translation="MEWSQIFHDITTKHDFKAMHDFLEKEYSTAIVYPDRENIYQAFD
                     LTPFENIKVVILGQDPYHGPNQAHGLAFSVQPNAKFPPSLRNMYKELADDIGCVRQTP
                     HLQDWAREGVLLLNTVLTVRQGEANSHRDIGWETFTDEIIKAVSDYKEHVVFILWGKP
                     AQQKIKLIDTSKHCIIKSVHPSPLSAYRGFFGSKPYSKANAYLESVGKSPINWCESEA
                     "
     gene            7628..8008
                     /locus_tag="P6L01_001760"
     CDS             7628..8008
                     /locus_tag="P6L01_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5327 family protein"
                     /protein_id="HDP3030827.1"
                     /translation="MVNRETLIARIEQELVQAEQAQHDHDFEKHMYAIHILTSLYAST
                     SNTPHIGEQQMNRRIANHNQMPQSQITQPTHQVTAAEIEAMGGKVNTHSAHHHNKSYS
                     QPSNQQQRLATDDDIGNGESIFDF"
     gene            8141..>8427
                     /locus_tag="P6L01_001761"
     CDS             8141..>8427
                     /locus_tag="P6L01_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDP3030828.1"
                     /translation="MKLFIILGALNAMMAVGTGAFGAHGLQGKISDHYLSVWEKATTY
                     QMYHGLALLIIGVISGTTSINVNWAGWLIFAGIIFFSGSLYILVLTQIKVLG"
BASE COUNT         2897 a         1285 c         1537 g         2708 t
ORIGIN      
        1 atgaattcat ggattttaat aaagagaata ttaacatggt ggatgcaaag aaagctaaaa
       61 aaaccgttgt tgcaaccggt atcggtaatg caatggaatg gttcgatttt ggtgtctatg
      121 catatacaac tgcgtacatt ggagcgaact tcttctctcc agtagagaat gcagacattc
      181 gacaaatgtt gactttcgca gcattagcca ttgcgttttt attaagacca attggtggtg
      241 tggtatttgg tattattggt gacaaatatg gacgtaaagt tgtattaaca tctacaatta
      301 ttttaatggc attttcaaca ttaaccattg gattattgcc aagctatgat caaattggac
      361 tttgggcacc aatactatta ttgcttgcaa gagtactaca agggttttca acaggtggag
      421 agtatgcggg ggcaatgaca tatgttgcgg agtcatctcc agataagcgt cgtaactcat
      481 taggtagtgg actagaaatt gggacattat caggttacat agctgcttca attatgattg
      541 cagtattaac attcttttta acagatgaac aaatggcatc atttggttgg agaatcccat
      601 tcttacttgg tttattccta ggattattcg acttatattt acgtcgtaag cttgaagaat
      661 caccagtttt cgaaaatgat gttgcaacac aaccagaacg agacaacatt aactttttac
      721 aaatcatcag attttattac aaagatatat ttgtgtgttt tgtagctgtt gtattcttca
      781 atgttacgaa ctatatggta actgcatatt taccaaccta tttagaacaa gttattaaat
      841 tagatgcaac gacaacaagt gtattaatta cttgtgtcat ggcaataatg attccattag
      901 cattaatgtt tggtaagtta gcggataaaa taggtgaaaa gaaagtattt ctaattggta
      961 ctggtgggct aacattattt agtatcatcg catttatgtt attacattca caatcatttg
     1021 ttgtaatagt aatcggtata tttatattag gatttttctt atcaacttac gaagcgacaa
     1081 tgccagggtc gttaccaacg atgttttaca gtcatataag atatcgaact ttatcagtaa
     1141 catttaatat ctctgtttcg atatttggtg gtacgacgcc attagttgca acatggttag
     1201 ttacgaaaac tggagatcca ttagcacctg cgtattattt aacagcaatc agtgttattg
     1261 gctttttagt tattacattc ttacatttaa gtacagcagg aaaatctcta aaaggttcgt
     1321 atccaaatgt agataacgag caagatagag cttattatgc agaacaccca aaagaagcat
     1381 tatggtgggt taaagaacgt aagaattaga gattttaata aaaagtataa atcaatcgta
     1441 tataagcact ttaaagctag taggttctgc taactttaaa gtgcttttta aattgagaac
     1501 tgtaattagc cgtaataaag tttttgtata tacataaacc cccactgcaa tgattatcgc
     1561 aatgggggaa agaggggact taaagcatat gtttagcttt gaatacttaa aattctcttg
     1621 ctattgaaat gttaggatgt aaatatgtct tagagtattt tgtccaacgc aattaatatt
     1681 gagactctaa ccttcaatat tattatagag aacacaaact taaatagatt gggtgactta
     1741 tttgtgtcag ttattgcgat tgcgataact tcttttctct atatacatat agtaacgtct
     1801 tatctaataa aaaacatggt actacagtat caaatttatc tagggcttaa gtttgatttt
     1861 tataataggc aggtttacct gataaaaata cttattcatt atataatgtt aacaagatgt
     1921 attttaaagt ttacattgag tgagggatat tgatgaacgt aattttagaa cagttgaaaa
     1981 cacatactca aaataaacct aatgacatag cattacatat cgatgatgaa acaattacat
     2041 atagtcaact aaatgcccgc atcactagcg cagttgaatc tttgaagaaa tattcactta
     2101 accctgtcgt tgctattaat atgaaatcac cggtgcaaag tattatttgt tatttagctt
     2161 tgcatcgttt acataaagtg cctatgatga tggaaggtaa atggcaaagt actatacatc
     2221 gtcaattgat tgaaaaatat ggtattaaag atgtaattgg agatacaggt ctcatgcaga
     2281 atatagactc accgatgttt attgattcaa cgcaattaca gcactacccc aatttattac
     2341 atattggttt tacttcaggg acaactggac tgccaaaagc atattatcgt gatgaagatt
     2401 catggttggc ttcttttgaa gttaatgaaa tgttgatgtt aaaaaatgaa aatgcaatag
     2461 cagcccctgg accactatcg cactcgttaa cattatatgc gttattgttt gctttaagtt
     2521 ccggtcgtac ttttatagga cagaccactt ttcatcctga aaagttactt aatcaatgtc
     2581 ataaaatatc atcatacaaa gttgctatgt ttcttgttcc aacgatgatt aaatcattat
     2641 tgttagttta caacaatgaa catacaatcc aatcattttt tagcagtgga gataagctgc
     2701 attcttctat ttttaaaaag ataaaaaatc aagcaaatga cataaatttg attgaatttt
     2761 ttggtacatc ggaaaccagt tttatcagct ataacttgaa tcagcaagca ccagttgaat
     2821 cagtaggtgt gctatttcca aatgtggaat tgaaaacaac gaatcacgat cacaatggta
     2881 taggaactat ttgtataaaa agtaatatga tgtttagtgg ctatgtaagt gaacaatgta
     2941 taaataatga tgaatggttt gttactaatg ataatggcta tgtaaaagag cagtatttat
     3001 atttaacggg acgtcaacag gatatgttaa ttattggtgg tcaaaatata tatccagcac
     3061 atgttgaacg ccttttaacg caatcttcga gcattgatga agcaattatc atcggtattc
     3121 caaatgagcg ttttggtcaa ataggcgtat tgctttattc tggtgatgtg acacttacac
     3181 ataaaaatgt aaaacaattt ttaaaaaaga aagtgaaacg ctatgaaatt ccatcgatga
     3241 ttcatcatgt agaaaagatg tattacactg caagtggtaa aattgctaga gaaaaaatga
     3301 tgtcgatgta tttgagaggt gaattataat atgaatcaag cagtcatagt tgcagctaaa
     3361 cgaactgcat ttgggaaata tggtggcact ttaaaacatt tagagccaga acaattgctt
     3421 aaacctttat tccaacattt taaagagaag tatccagagg taatatctaa aatagatgat
     3481 gtagttttag gtaatgttgt tgggaatggt ggcaatattg caagaaaagc attgcttgaa
     3541 gcggggctta aagattcaat acctggcgtc acaatcgatc ggcaatgtgg gtctggactt
     3601 gaaagtgttc aatatgcatg tcgcatgatc caagccggag ctggcaaggt atatattgca
     3661 ggtggtgttg aaagtacaag tcgagcacct tggaaaatca aacgaccgca ttctgtgtac
     3721 gaaacagcat tacctgagtt ttatgagcgt gcatcatttg cacctgaaat gagcgaccca
     3781 tcaatgattc aaggtgctga aaatgtggcc aagatgtatg atgtttcaag agaattacaa
     3841 gatgaatttg cttatcgaag tcatcaactg acagcggaaa atgtaaagaa tggaaatatt
     3901 tctcaggaaa tattacctat aaccgttaaa ggagaaatat tcaatactga tgaaagtcta
     3961 aaatcacata ttccgaaaga taactttggc cgatttaagc ccgtaatcaa aggtgggacc
     4021 gttaccgctg cgaatagttg tatgaaaaat gatggtgcag ttttattgct tattatggaa
     4081 aaagatatgg catacgaatt aggtttcgag catggtttat tatttaaaga tggtgttacg
     4141 gtaggtgttg attctaattt tcctggcatt ggtccagtac cagccatttc caacttacta
     4201 aaaagaaatc aattaacgat agaaaatatt gaagtcattg aaattaacga agcgttcagt
     4261 gcacaggtag ttgcctgcca acaagcttta aatatttcaa atacgcaatt aaatatatgg
     4321 ggtggtgcat tagcatcagg tcatccatac ggtgcaagcg gtgcccaatt agtgactcga
     4381 ttattttata tgtttgacaa agagactatg attgcatcta tggggatagg gggaggtcta
     4441 ggaaatgcag cattatttac tcgattctaa ccagcgatta aatgtgtcat tttctaagga
     4501 tagtgtggct gcatattatc agtgttgtaa ccaaccttat agaaaagaag taccaccatt
     4561 aatgtgtgcg tcattatggc caaaatttga tttatttaaa aaatatgcaa atagcgaact
     4621 gattttaaca aaatcagcaa ttaatcaaac tcaaaagata gaagtagaca caatatatgt
     4681 agggcattta gaagatattg aatgccgaca gactcgcaat atcacacgtt atacaatggc
     4741 tttaacatta actaaaaatg atcaacatgt cataacggtt acacaaactt ttattaaggc
     4801 gatgaagtag agatgaagtt taatgagata tggataaatg aatatttggc gctcgtaaat
     4861 gatgataatc caatacataa tgagattgtg ccaggacaat tagtgagtca aatgatgctg
     4921 atggctatgt cattagagac aaaccagtgt caaattaact acgttaaacc tattttaata
     4981 aatgaaaata tcgaattcat tgaacaacac gaacacgaaa ttatagcaat taatgacgat
     5041 ggagagatta aaataaaaat ttctttgagc acaaaaaaat aaccgatatt agctgcatga
     5101 acgcatatta attaggagat gaaaggacag ctaatatcag ttatgtattg ttattattat
     5161 tgggaacaga gatgaatata ggttacgttt ctttctttgc acggggatgc attaatctaa
     5221 aataataata acaactatat caatgtttaa taaattctgg attattggaa cgattagtca
     5281 atttaactaa ctttcatatg atctatatcg tcttgtaata aagagagcaa tttgaatatt
     5341 tcagtatcac taaatgaatc gtcacattta attgaaacat gctgaaacgt tttggttata
     5401 atttcataaa ctggtgcgcc ttcatggtga tactgtcgat aaataatcat aacctatatt
     5461 acctcctttg ctactctatg gttatattat aaataacatt tttatgtgtg acatcaacct
     5521 taagtatcaa ctttttatca gacatagaac gtatgattta ctaagactat ttatgtataa
     5581 aagttctaaa taaatatata tttatagagt cgcctggcag tcatttggga aatataacat
     5641 atatgattag agaggcatct atcgcaaaag aatgataatg atagaggtat tgagcatata
     5701 gatgagttta agttcatctt gaaaataaag ggttatttag tcatagatgt agatgtatag
     5761 gaaatatttg tatgtattgt tcgatatgta tgaaattttc aataaaagct aataacgctt
     5821 atatgtaact ttcaaattta aattatatac agagcatgat gattataaaa aaataaccac
     5881 atcacataaa ttgagttcat acccaattta agtggtgtgg ctaataatgt tgatttatag
     5941 atgaaccgcc taatcgttaa acctctgtta cttcaacatc gatatgttca atacggttgt
     6001 atgcaccgtg atccacagga ccaacaaaat cattcatttt ccaaccgttt ttaatagcag
     6061 aagcgacgaa agctttcgcg ctaatcacag cttctttcgg tgacttaccg ttagctaaat
     6121 atgcagttgt tgccgcagca aatgtacaac cagcaccatg gttataactt tgttggaaca
     6181 tgtctgttgt tagttgataa aatgtttgac catcatagta taagtcatac gatttatctt
     6241 gatctaaagc tttgccacct ttaatgatga catgctgtgc gcctttatca aagataattg
     6301 ttgcagcctt tttcatatct tcaattgaat ttaatttacc taatcctgat aattgacccg
     6361 cttcaaataa gtttggtgtc actaccgttg ctttaggtag taaatattta atcatcgcct
     6421 cagtatttcc aggattaagc acttcatctt cgcctttaca aaccatgaca ggatctacta
     6481 caaaatattg tgcattagat gcctcatata cttctccagc acgtttgatt atctcctcag
     6541 tacctaacat acctgtttta atagcatcag gtccgattga taaagccgtt tcaagttgtt
     6601 tttcaaatac atccattggt aatggtgtaa catcgtgtga ccatgtatct ttatccatag
     6661 taacgatggc agttaaagcg accatgccat acgtatctaa ttcttggaac gttttcaaat
     6721 ctgcttgcat acctgcgcca gcacttgtgt cagaaccggc aattgttaaa actttcttta
     6781 aagccattga gcttcactcc tacataataa tattgtattc atcatatcat ttttaaccta
     6841 attgaaaaat attaagcatt caatatttga tgattgttga aatgaatcat tcatactatt
     6901 gtaacttttg aaaatgtcat tcactttaga taagtgtgat atgttaaaat atgtcctgag
     6961 gtgagattga atggaatggt cgcaaatttt tcatgacata acaacgaaac atgactttaa
     7021 agctatgcat gattttttag aaaaagaata ttcgactgca atcgtatacc ctgataggga
     7081 aaatatatat caagcgtttg atttaacacc gtttgaaaat atcaaagttg ttatattagg
     7141 acaagacccg tatcatggtc caaaccaagc acatggatta gcattttcag tgcaacctaa
     7201 cgcaaaattc cctccatctt tacgtaatat gtataaagaa ttagcagatg atattggatg
     7261 cgttagacaa acaccgcatt tacaagattg ggcaagagaa ggcgtcttgt tattgaatac
     7321 agttttaacc gtaagacagg gtgaagcaaa ttctcatcgt gatattggtt gggaaacatt
     7381 tactgatgaa attattaaag cagtgtctga ttataaagaa catgttgtct ttattttgtg
     7441 ggggaaacct gcacagcaaa aaataaagct tatcgataca tctaaacatt gtattataaa
     7501 atcagtgcat cctagtccac tgtctgcata tagaggattc tttggatcaa aaccgtattc
     7561 caaagcgaat gcctatttag agtcagtagg aaaatcacca attaattggt gtgaaagtga
     7621 ggcgtagatg gtgaatagag aaactttaat agcacgaatt gagcaagaat tagtacaagc
     7681 agagcaggca cagcatgacc atgactttga aaaacatatg tatgccatac atatattaac
     7741 atctttatat gcttcaacat caaatacacc acatattggt gaacaacaaa tgaatcgtcg
     7801 tattgctaac cataatcaaa tgccacaatc acaaataacg cagccaactc atcaagtgac
     7861 agctgctgaa attgaagcga tgggtggtaa agtaaatacg cattcagcac atcatcataa
     7921 taagtcatat tcacaacctt caaaccaaca acaaagatta gcgacagatg atgacattgg
     7981 caatggtgaa tccatatttg atttttaaaa agcaacaatg aaacataatt acttaatagc
     8041 ttgttaagta tgttggttaa taatcaagac gcatatactt ttattcgagt gttcggattt
     8101 aaacatttat taatactgaa ttatataagg agaggtagca atgaaattat ttattatttt
     8161 aggtgcatta aacgcgatga tggctgtcgg aacaggtgca tttggtgcgc atggtttaca
     8221 aggaaaaata agtgatcact atttatcagt atgggaaaaa gcaacgacgt atcaaatgta
     8281 ccatggctta gcattattaa ttataggtgt aattagtggt acaacttcaa tcaatgttaa
     8341 ctgggctggc tggttaatat ttgctggtat tattttcttt agtggatcat tatatatttt
     8401 agtattaact caaattaaag ttttagg
//
LOCUS       DAOBBH010000107         8396 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.107, whole genome shotgun sequence.
ACCESSION   DAOBBH010000107 DAOBBH010000000
VERSION     DAOBBH010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8396
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.107"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            126..6686
                     /gene="sasC"
                     /locus_tag="P6L01_001762"
     CDS             126..6686
                     /gene="sasC"
                     /locus_tag="P6L01_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored repetitive surface protein SasC"
                     /protein_id="HDP3030829.1"
                     /translation="MNLLKKNKYSIRKYKVGIFSTLIGTVLLLSNPNGAQALTTDNNV
                     QSDTNQATPVNSQDTNVANNRGLANSAQNTPNQSATTNQSTNQALVNHNNGSIANQAT
                     PTSVQSSTPSAQNNNHTDGNTTATETVSNTNNKDVVSNNTTLNVPNKTNENGSGGHLT
                     LKEIQEDVRHSSDKPELVAIAEQASNRPKKRSRRAAPADPNATPADPAAAAAGNGGAP
                     VAITAPYTPTTDPNANNAGQNAPNEVLSFDDNGIRPSTNRSVPSVTVVDNLPGFTLIN
                     GGKVGVFSHAMVRTSMFDSGDAKNYQAQGNVIALGRIKGNDTNDHGDFNGIEKSLTVN
                     PNSELIFEFNTMTTKNYQGVTNLIIKNADNDTVIAEKSVAYGPIWRLFKVPENVSHLK
                     IQFVPKNDAITDARGIYQLRDGYKYYDFVDSIGLHSGSHVYVERRTMEPTATNNKEFT
                     VTTSLKNNGNFGASFNTDDFVYKVQLPEGVEYVNNSLTKDFPSSNSGVDMNDFNVTYD
                     AANRVITIKSTGGGSGNSPARLMPDKILDLKYKLRVNNVPTPRTVTFNDTLTYKTYTQ
                     DFINSPAESHTVSTNPYTIDIIMNKDALQAEVDRRIQQADYTFASLDIFNDLKRRAQT
                     ILDENRNNVPLNKRVSQADIDSLTNQMQHTLIRSVDAENAVNKKVDQMEDLVNQNDEL
                     TDEEKQAAIQVIEEHKNEIIGNIGDQTTDDGVTRIKDQGIQTLSGDTATPVVKPNAKK
                     AIRDKATKQREIINATPDATEDEIQDALNQLATDETDAIDNVTNATTNADVEIAKNNG
                     INTIGAVVPQVTHKQAARDAINQATATKRQQINSNREATQEEKNAALNELTQATNHAL
                     EQINQATTNADVDNAKGDGLNAINPIAPVTVVKQAARDAVSHDAQQHIAEINANPDAT
                     QEERQAAIDKVNAAVTAANTNILNANTNADVEQVKTNAIQGIQAITPATKVKTDAKNA
                     IDKSAETQHNTIFNNNDATLEEQQAAQQLLDQAVATAKQNINAADTNQEVAQAKDQGT
                     QNIVVIQPATQVKTDARNAVNDKAREAITNINATPGATREEKQEAINRVNTLKNRALT
                     DIGVTSTTAMVNSIRDDAVNQIGAVQPHVTKKQTATGVLNDLATAKKQEINQNTNATT
                     EEKQVALNQVDQELATAINNINQADTNAEVDQAQQLGTKAINAIQPNIVKKPAALAQI
                     NQHYNAKLAEINATPDATNDEKNAAINTLNQDRQQAIESIKQANTNAEVDQAATVAEN
                     NIDAVQVDVVKKQAARDKITAEVAKRIEAVKQTPNATDEEKQAAVNQINQLKDQAINQ
                     INQNQTNDQVDTTTNQAVNAIDNVEAEVVIKPKAIADIEKAVKEKQQQIDNSLDSTDN
                     EKEVASQALAKEKEKALAAIDQAQTNSQVNQAATNGVSAIKIIQPETKVKPAAREKIN
                     QKANELRAKINQDKEATAEERQVALDKINEFVNQAMTDITNNRTNQQVDDTTSQALDS
                     IALVAPEHIVRAAARDAVKQQYEAKKQEIEQAEHATDEEKQVALNQLANNEKLALQNI
                     NQAVTNNDVKRVETNGIATLKGVQPHIVIKPEAQQAIKATAENQVESIKDTPHATVDE
                     LDEANQLISDTLKQAQQEIENTNQDAAVTDVRNQTIKAIEQIKPKVRRKRAALDSIEE
                     NNKNQLDAIRNTLDTTQDERDVAIDTLNKIVNTIKNDIAQNKTNAEVDRTETDGNDNI
                     KVILPKVQVKPAARQSVGVKAEAQNALIDQSDLSTEEERLAAKHLVEQALNQAIDQIN
                     HADKTAQVNQDSIDAQNIISKIKPATTVKATALQQIQNIATNKINLIKANNEATDEEQ
                     NIAIAQVEKELIKAKQQIASAVTNADVAYLLHDEKNEIREIEPVINRKASAREQLTTL
                     FNDKKQAIEANIQATVEERNSILAQLQNIYDTAIGQIDQDRSNAQVDKTASLNLQTIH
                     DLDVHPIKKPDAEKTINDDLARVTALVQNYRKVSNRNKADALKAITALKLQMDEELKT
                     ARTNADVDAVLKRFNVALSDIEAVITEKENSLLRIDNIAQQTYAKFKAIATPEQLAKV
                     KVLIDQYVADGNRMIDEDATLNDIKQHTQFIVDEILAIKLPTEATKVSPKEIQPAPKV
                     CTPIKKEETHESRKVEKELPNTGSEGMDLPLKEFALITGAALLARRRTKNEKES"
     gene            complement(6803..8071)
                     /locus_tag="P6L01_001763"
     CDS             complement(6803..8071)
                     /locus_tag="P6L01_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDP3030830.1"
                     /translation="MYQTIIIGGGPSGLMAAVAASEQSSSVLLIEKKKGLGRKLKISG
                     GGRCNVTNRLPYAEIIKNIPGNGKFLYSPFSIFDNESIIDFFESRGVKLKEEDHGRMF
                     PVSNKAQDVVDTLVTTIERQHVTIKEEEAVSRIEVNTDQTFTVHTQNNSYESHSLVIA
                     TGGTSVPQTGSTGDGYKFAQDLGHTITELFPTEVPITSAEPFIKSNRLKGLSLKDVEL
                     SVLKKNGKKRISHQMDMLFTHFGISGPAALRCSQFVYKEQKNQKTQHISMAIDAFPEL
                     NHEQLKQHITSLLSDTPDKIIKNSLHGLIEERYLLFMLEQAGIDENTTSHHLSNQQLN
                     DLVNMFKGFVFKVNGTLPIDKAFVTGGGVSLKEIQPKTMMSKLVPGLFLCGEVLDIHG
                     YTGGYNITSALVTGHVAGLYAGHYSHTSME"
BASE COUNT         3308 a         1394 c         1482 g         2212 t
ORIGIN      
        1 aaaattgagt gaacttaaaa tattgtacaa aataatatag ctataaacat aatatgagag
       61 agcgtatatt tttagcataa ttctaaacaa cacagcagag aacagacaac caggaggaaa
      121 atgaaatgaa tttgttaaag aaaaataaat atagtattag gaagtataaa gtaggcatat
      181 tctctacttt aatcggaaca gttttattac tttcaaaccc aaatggtgca caagccttaa
      241 ctacggataa taatgtacaa agcgatacta atcaagccac acctgtaaat tcacaagata
      301 caaatgttgc taataataga ggtttagcaa atagtgctca gaatacacct aatcaatctg
      361 caacaactaa tcaatcaacg aatcaagcat tggttaatca taataatggt agtatagcaa
      421 atcaagctac gccaacatca gtgcaatcaa gtacgccttc agcacaaaac aataatcata
      481 cagatggcaa tacaacagca actgagacag tgtcaaacac taataataag gatgtagtgt
      541 cgaataatac cacattaaat gtacctaata aaacaaatga aaatggttca ggaggacatc
      601 taactttaaa ggaaattcaa gaagatgttc gtcattcttc agataaacca gagctagttg
      661 caattgctga acaagcatct aatagaccga aaaagagaag tagacgtgcg gcaccggcag
      721 atcctaatgc aactccagca gatccagcgg ctgcagcggc aggaaacggt ggtgcaccag
      781 tagcaattac agcgccatac acgccgacaa ctgatcctaa tgccaataat gcaggacaaa
      841 atgcacctaa cgaagtgctg tcttttgatg acaatggtat tagaccaagt accaaccgtt
      901 ctgtaccatc agtaacagtt gttgataatt taccaggctt tacacttatc aatggtggta
      961 aagtaggtgt gtttagtcat gcgatggtta gaacaagtat gtttgattca ggagatgcta
     1021 agaactatca agcacaagga aatgtaattg cattaggtcg tattaaggga aatgatacaa
     1081 atgatcatgg tgattttaat ggaattgaaa aatctttaac agtaaatcca aattctgaat
     1141 taattttcga atttaatacg atgactacaa aaaactatca aggtgtgaca aatttaataa
     1201 ttaaaaatgc tgataatgat actgttattg cagaaaaatc agtagcttat ggtccgattt
     1261 ggcgtttgtt taaagtgcct gaaaatgtta gtcacttaaa aattcaattt gtacctaaaa
     1321 atgatgcaat aacagatgcg cgtggcattt atcaactaag agacggttat aaatactatg
     1381 actttgttga ctcaattggt ctacattctg gttcgcatgt ctatgttgaa agacgaacaa
     1441 tggaacccac agctactaat aataaagaat ttacagtcac aacttcatta aagaataatg
     1501 gtaacttcgg tgcttcattt aatacagatg attttgtata taaagttcaa ttacctgaag
     1561 gggtagaata tgttaataac tcattgacta aagattttcc aagcagtaat tcaggtgttg
     1621 atatgaatga ttttaatgtg acgtatgatg cggcgaatcg agtgataaca attaaaagta
     1681 ctggtggagg ttcaggtaac tcgccggcac gattaatgcc tgataaaata ttggatttaa
     1741 agtataagtt gcgtgttaat aatgtaccga caccaagaac agtaacattt aatgatacat
     1801 taacatataa aacatataca caagatttta ttaattcacc tgctgaaagt catactgtaa
     1861 gtacaaatcc atatacaatc gatatcatca tgaataaaga tgcattacaa gccgaggttg
     1921 atagacgcat tcaacaagct gattatacat ttgcatcatt agatatcttt aatgatctta
     1981 aaagacgtgc acaaacgatt ttagatgaaa atcgtaacaa tgtaccatta aataaaagag
     2041 tttctcaagc agatattgat tcattaacta atcaaatgca acatacgtta attcgaagtg
     2101 ttgatgctga aaatgcagtt aataaaaaag ttgaccaaat ggaagattta gttaatcaaa
     2161 atgatgaatt gacagatgaa gaaaaacaag cagcaataca agttatcgag gaacataaaa
     2221 atgaaataat tggtaatatt ggtgaccaaa cgactgatga tggcgttact agaatcaaag
     2281 atcaaggtat acagacctta agtggggata ctgcaacacc ggttgttaaa ccaaatgcta
     2341 aaaaagcaat acgtgataaa gcaacgaaac aaagggaaat tatcaatgca acaccagatg
     2401 ctactgaaga cgagattcaa gatgcactaa atcaattagc tacggatgaa acagatgcta
     2461 ttgataatgt tacgaatgct actacaaatg ctgacgttga aatagctaaa aataatggca
     2521 tcaatactat tggagcagtt gttcctcaag tgacacataa acaagctgca agagatgcaa
     2581 ttaaccaagc aacagcaacg aaaagacaac aaataaatag taatagagaa gcaactcagg
     2641 aagagaaaaa tgcagcattg aacgaattaa ctcaagcaac caaccatgct ttagaacaaa
     2701 tcaatcaagc aacaacaaat gctgatgttg ataacgccaa aggagatggt ctaaatgcca
     2761 ttaatccaat tgctcctgta actgttgtta agcaagctgc aagggatgct gtatcacatg
     2821 atgcacaaca acatatcgca gaaatcaatg ctaatcctga tgcgactcaa gaagaaagac
     2881 aagcagcaat tgacaaagtg aatgctgctg taactgcagc aaacacaaac attttaaacg
     2941 ctaataccaa tgctgatgtt gaacaagtaa agacaaatgc gattcaagga atacaagcaa
     3001 ttacaccagc tacaaaagta aaaacagatg caaaaaatgc catcgataaa agtgcggaaa
     3061 cgcaacataa tacgatattt aataataatg atgcgacgct cgaagaacaa caagcagcac
     3121 aacaattact tgatcaagct gtagccacag cgaagcaaaa tattaatgca gcagatacga
     3181 atcaagaagt tgcacaagca aaagatcagg gcacacaaaa tatagttgtg attcaaccgg
     3241 caacacaagt taaaacggat gctcgcaatg ctgtaaatga taaagctcga gaggcgataa
     3301 caaatatcaa tgctacacct ggcgcgactc gagaagagaa acaagaagcg ataaatcgtg
     3361 tcaatacact taaaaataga gcattaactg atattggtgt gacgtctact actgcgatgg
     3421 tcaatagtat tagagacgat gcagtcaatc aaatcggcgc agttcaaccg catgtaacga
     3481 agaaacaaac tgctacaggt gtattaaatg atttagcaac tgctaaaaag caagaaatta
     3541 atcaaaacac aaatgcaaca actgaagaaa agcaagtggc tttaaatcaa gtggatcaag
     3601 agttagcaac ggcaattaat aatataaatc aagctgatac aaatgcggaa gtagatcaag
     3661 cgcaacaatt aggtacaaaa gcaattaatg cgattcagcc aaatattgtt aaaaaacctg
     3721 cagcattagc acaaatcaat cagcattata atgctaaatt agctgaaatc aatgctacac
     3781 cagatgcaac gaatgatgag aaaaatgctg cgatcaatac tttaaatcaa gacagacaac
     3841 aagctattga aagtattaaa caagctaaca caaatgcaga agtagaccaa gctgcgacag
     3901 tagcagagaa taatatcgat gctgttcaag ttgatgtagt aaaaaaacaa gcagcgcgag
     3961 ataaaatcac tgctgaagtg gcgaagcgta ttgaagcggt taaacaaaca cctaatgcaa
     4021 ctgacgaaga aaagcaggct gctgttaatc aaatcaatca acttaaagat caagcaatta
     4081 atcaaattaa tcaaaaccaa acaaatgatc aggtagacac aactacaaat caagcggtaa
     4141 atgctataga taatgttgaa gctgaagtag taattaaacc aaaggcaatt gcagatattg
     4201 aaaaagctgt taaagaaaag caacagcaaa ttgataatag tcttgattca acagataatg
     4261 agaaagaagt tgcttcacaa gcattagcta aagaaaaaga aaaagcactt gcagctattg
     4321 accaagctca aacgaatagt caggtgaatc aagcagcaac aaatggtgta tcagcgatta
     4381 aaattattca acctgaaaca aaagttaaac cagctgcacg tgaaaaaatc aatcaaaaag
     4441 cgaatgaatt acgtgctaag attaatcagg ataaagaagc aacagcagaa gaaagacaag
     4501 tagcactaga taaaatcaat gaatttgtaa atcaagccat gacagatatt acgaataata
     4561 gaacaaatca acaagttgat gatacaacaa gtcaagcgct tgatagcatt gctttagtag
     4621 cgcctgaaca tattgttaga gcagctgcta gagatgcagt aaagcaacaa tatgaggcta
     4681 aaaagcaaga aatagagcaa gctgaacatg cgactgatga agaaaaacaa gttgctttaa
     4741 atcaattagc gaataatgaa aaacttgcat tacaaaacat caatcaagca gtaacgaata
     4801 atgatgtgaa acgtgttgaa acaaatggca ttgctacact aaaaggtgta caacctcata
     4861 ttgtaattaa gcctgaagca caacaagcaa taaaagcaac tgcagaaaat caagtagaat
     4921 caataaaaga tacaccacat gcaacagttg atgaattaga tgaagcgaat caattaatta
     4981 gcgacacact caaacaagcg caacaagaaa tagaaaatac aaatcaagat gctgctgtta
     5041 ctgatgttag aaatcaaaca atcaaggcaa ttgagcaaat aaaacctaaa gtaagacgta
     5101 aacgagctgc gcttgatagc attgaagaaa ataataaaaa tcaactcgat gcaatccgaa
     5161 atacgttgga tactactcaa gatgaaagag atgttgctat tgatacttta aataaaattg
     5221 taaatacaat taaaaatgac attgcacaaa acaaaacgaa tgcagaagtg gatcgaactg
     5281 agactgatgg caacgacaac atcaaagtga ttttacctaa agttcaagtt aaaccagcag
     5341 cgcgtcaatc tgttggtgta aaagccgaag ctcaaaatgc actaatcgat caaagcgatt
     5401 tatcaactga agaagaaaga ctagctgcta aacatttggt agaacaagca cttaatcagg
     5461 ctattgatca gatcaatcat gcagataaga ctgcccaagt taatcaagat agtatagatg
     5521 ctcaaaatat tatttcaaaa attaaaccag cgacaacagt taaagcaaca gcattacaac
     5581 aaattcaaaa tatcgctaca aataaaatta atttaattaa agcaaataac gaagcgacag
     5641 atgaagaaca aaatattgca atagcacaag ttgaaaaaga gttaattaaa gctaaacaac
     5701 aaattgctag tgcagtgact aatgctgatg tggcatattt attgcatgat gagaaaaacg
     5761 aaattcgtga aatcgaacct gttattaaca gaaaggcgtc tgctcgagaa caattgacaa
     5821 cattattcaa cgataaaaaa caagcaattg aagcgaatat tcaagcaacg gtagaagaaa
     5881 gaaatagtat attagcacag ttacaaaata tttatgacac tgctattgga caaattgatc
     5941 aagatcgtag caatgcacaa gttgataaaa cagcatcatt aaatctacaa acaatacatg
     6001 atttagatgt acatcctatt aaaaagccag atgctgaaaa aacgattaat gatgatcttg
     6061 cacgcgtcac tgctttagtg caaaattatc gaaaagtaag taatcgtaat aaggctgatg
     6121 cattaaaagc tataactgct ttaaaattac aaatggatga agaattaaaa acagcacgca
     6181 ctaatgctga tgttgatgca gttttaaaac gatttaatgt tgcattaagc gatatagaag
     6241 cagtaattac tgaaaaagaa aatagcttac tgcgaattga taacattgct caacaaacat
     6301 atgcgaaatt caaagcgatc gcaacaccag aacaattagc taaagtaaaa gtattaattg
     6361 atcaatatgt tgcagatggc aatagaatga ttgatgaaga tgcgacatta aatgacatca
     6421 aacaacacac gcaattcatt gttgatgaaa ttttagcaat taaattacca actgaagcga
     6481 cgaaagtatc accaaaagaa attcagccag ctccaaaagt ttgtacgcct attaaaaaag
     6541 aagagacaca tgaatcgcgc aaagttgaaa aagaacttcc aaatacaggt tctgaaggaa
     6601 tggatttacc attgaaagaa tttgcactga ttacaggtgc ggctttgtta gctagaagac
     6661 gtactaaaaa cgaaaaagaa tcataattaa cgaaatgacc ttaatataac gacacttttt
     6721 agtgactcag ttatattaag gtcattttta attgaatgaa aaagttgatc tactaaagag
     6781 aatcgaacca aattttatac tattattcca ttgatgtatg tgagtaatga ccggcatata
     6841 atccagcgac atgtcctgtt acgagtgcac ttgtaatatt ataaccacca gtataaccat
     6901 gtatatctaa tacttcacca cataaaaata atcccggaac taatttagac atcattgttt
     6961 taggttgaat ttctttaagt gacacaccac cacctgtgac aaatgcctta tctataggta
     7021 atgtcccgtt taccttaaat acaaaccctt taaacatatt tactaagtcg ttcaattgtt
     7081 gatttgataa gtgatgtgat gtggtatttt catcgattcc agcttgttcc agcatgaaca
     7141 gtaagtagcg ctcttcaatt agaccatgca aactgttttt aatgatttta tctggtgtgt
     7201 ccgataataa tgatgtgatg tgttgtttta attgttcatg gtttaattca ggaaatgcat
     7261 cgattgccat agaaatgtgc tgtgtctttt gatttttttg ttctttataa acaaactgac
     7321 tacatcttaa tgcagctgga ccactgatac caaaatgagt aaataacata tccatttgat
     7381 gactgatgcg ttttttacca tttttcttaa gtactgacaa ttcaacatct tttaaactta
     7441 aaccttttag acgattggat ttgatgaaag gttcagctga tgtaattgga acttcggtcg
     7501 ggaataactc agtaatggta tgacctaaat cttgtgcgaa cttataacca tcaccagttg
     7561 aaccagtttg agggacactt gtaccacctg tagcaatcac tagcgaatgg ctttcataac
     7621 tattattttg agtatgtaca gtgaaagttt ggtctgtatt aacttcgatt ctactaacag
     7681 cttcttcttc tttaatcgtt acatgttggc gttcgatagt tgtcactaat gtatcaacca
     7741 cgtcttgtgc tttgttggaa actggaaaca tacgcccgtg atcttcttct tttaatttaa
     7801 cacccctaga ctcaaaaaaa tctatgatgg attcattatc aaaaattgaa aagggactat
     7861 ataaaaattt cccatttcca ggaatgttct taataatttc agcatatggt aatcgattag
     7921 ttacgttaca tctaccgcca ccagatattt tgagtttacg acctagacct ttcttttttt
     7981 caatgagtaa cacactgcta ctttgttcgc ttgcagctac tgccgccatt aagccgctag
     8041 gtccgcctcc gataataatt gtttgataca tatacgcttc ctccaaattt gttactggac
     8101 attcaattgc tattttatag tactttttta aactttgcta tatctcttgt gttatgagat
     8161 gacaattatg ttgaatatat ttataatgtg aacttcataa aaaatgaaga tgtatgtgta
     8221 tgataagaat gatttttcta ctgttatgcg atgttcatct caaatagtag tataatgttt
     8281 atatgaaatt tcgtaatatt ctaatcaaaa tcgagtattc aatgacaatg cgttgataat
     8341 tttgttatga taattcattg aatatacgac aatgaaataa agttttgtga tatatt
//
LOCUS       DAOBBH010000108         8354 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.108, whole genome shotgun sequence.
ACCESSION   DAOBBH010000108 DAOBBH010000000
VERSION     DAOBBH010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8354
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.108"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(89..1816)
                     /locus_tag="P6L01_001764"
     CDS             complement(89..1816)
                     /locus_tag="P6L01_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3030831.1"
                     /translation="MKRENPLFFLFKKLSWPVGLIVAAITISSLGSLSGLLVPLFTGR
                     IVDKFSVSHINWNLIALFGGIFVINALLSGLGLYLLSKIGEKIIYAIRSVLWEHIIQL
                     KMPFFDKNESGQLMSRLTDDTKVINEFISQKLPNLLPSIVTLVGSLIMLFILDWKMTL
                     LTFITIPIFVLIMIPLGRIMQKISTSTQSEIANFSGLLGRVLTEMRLVKISNTERLEL
                     DNAHKNLNEIYKLGLKQAKIAAVVQPISGIVMLLTIAIILGFGALEIATGAITAGTLI
                     AMIFYVIQLSMPLINLSTLVTDYKKAVGASSRIYEIMQEPIEPTEALEDSENVLIDDG
                     VLSFEHVDFKYDVKKILDDVSFQIPQGQVSAFVGPSGSGKSTIFNLIERMYEIESGDI
                     KYGLESVYDIPLSKWRRKIGYVMQSNSMMSGTIRDNILYGINRHVSDEELINYAKLAN
                     CHDFIMQFDEGYDTLVGERGLKLSGGQRQRIDIARSFVKNPDILLLDEATANLDSESE
                     LKIQEALETLMEGRTTIVIAHRLSTIKKAGQIIFLDKGQVTGKGTHSELMASHAKYKN
                     FVVSQKLTD"
     gene            2237..3532
                     /gene="pbp4"
                     /locus_tag="P6L01_001765"
     CDS             2237..3532
                     /gene="pbp4"
                     /locus_tag="P6L01_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP4"
                     /protein_id="HDP3030832.1"
                     /translation="MKNLISIIIILCLTLSIMTPYAQATNSDVTPVQAANQYGYAGLS
                     AAYEPTSAVNVSQTGQLLYQYNIDTKWNPASMTKLMTMYLTLEAVNKGQLSLDDTVTM
                     TNKEYIMSTLPELSNTKLYPGQVWTIADLLQITVSNSSNAAALILAKKVSKNTSDFVD
                     LMNNKAKAIGMKNTHFVNPTGAENSRLRSFAPTKYKDQERTVTTARDYAILDLHVIKE
                     TPKILDFTKQLAPTTHAVTYYTFNFSLEGAKMSLPGTDGLKTGSSDTANYNHTITTKR
                     GKFRINQVIMGAGDYKNLGGEKQRNMMGNALMERSFDQYKYVKILSKGEQRINGKKYY
                     VENDLYDVLPSDFSKKDYKLVVEDGKVHADYPREFINKDYGPPTVEVHQPIIQKANTV
                     AKSMWEEHPLFTIIGGACLVAGLALIVHMIINRLFRKRK"
     gene            complement(3649..4047)
                     /gene="tagD"
                     /locus_tag="P6L01_001766"
     CDS             complement(3649..4047)
                     /gene="tagD"
                     /locus_tag="P6L01_001766"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HDP3030833.1"
                     /translation="MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIK
                     HKKSYYDYEQRKMMLESIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFL
                     KDKCEVIYLKRTEGISTTKIKQELYGKDAK"
     gene            complement(4110..5171)
                     /locus_tag="P6L01_001767"
     CDS             complement(4110..5171)
                     /locus_tag="P6L01_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDP3030834.1"
                     /translation="MRLTIIIPTCNNEATIRQLLISIESKEHYRILCIDGGSTDQTIP
                     MIERLQRELKHISLIQLQNASIATCINKGLMDIKMTDPHDSDAFMVINPTSIVLPGKL
                     DRLTAAFKNNDNIDMVIGQRAYNYHGEWKLKSADEFIKDNRIVTLTEQPDLLSMMSFD
                     GKLFSAKFAELQCDETLANTYNHAILVKAMQKATDIHLVSQMIVGDNDIDTHATSNDE
                     DFNRYIIEIMKIRQRVMEMLLLPEQRLLYSDMVDRILFNNSLKYYMNEHPAVTHTTIQ
                     LVKDYIMSMQHSDYVSQNMFDIINTVEFIGENWDREIYELWRQTLIQVGINRPTYKKF
                     LIQLKGRKFAHRTKSMLKR"
     gene            complement(5168..6271)
                     /locus_tag="P6L01_001768"
     CDS             complement(5168..6271)
                     /locus_tag="P6L01_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDP3030835.1"
                     /translation="MNVLIKKFYHLVVRILSKMITPQVIDKPHIVFMMTFPEDIKPII
                     KALNNSSYQKTVLTTPKQAPYLSELSDDVDVIEMTNRTLVKQIKALKSAQMIIIDNYY
                     LLLGGYNKTSNQHIVQTWHASGALKNFGLTDHQVDVSDKAMVQQYRKVYQATDFYLVG
                     CEQMSQCFKQSLGATEEQMLYFGLPRINKYYTADRATVKAELKDKYGITNKLVLYVPT
                     YREDKADNRAIDKAYFEKCLPGYTLINKLHPSIEDSDIDDVSSIDTSTLMLMSDIIIS
                     DYSSLPIEASLLDIPTIFYVYDEGTYDKVRGLNQFYKAIPDSYKVYTEEDLIMTIQEK
                     EHLLSPLFKDWHKYNTDKSLHQLTEYIDKMVTK"
     gene            complement(6370..7182)
                     /gene="tagG"
                     /locus_tag="P6L01_001769"
     CDS             complement(6370..7182)
                     /gene="tagG"
                     /locus_tag="P6L01_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter permease
                     subunit TagG"
                     /protein_id="HDP3030836.1"
                     /translation="MSAIGTVFKEHVKNFYLIQRLAQFQVKIINHSNYLGVAWELINP
                     VMQIMVYWMVFGLGIRSNAPIHGVPFVYWLLVGISMWFFINQGILEGTKAITQKFNQV
                     SKMNFPLSIIPTYIVTSRFYGHLGLLLLVIIACMFTGIYPSIHIIQLLIYVPFCFFLT
                     ASVTLLTSTLGVLVRDTQMLMQAILRILFYFSPILWLPKNHGISGLIHEMMKYNPVYF
                     IAESYRAAILYHEWYFMDHWKLMLYNFGIVAIFFAIGAYLHMKYRDQFADFL"
     gene            7529..8323
                     /gene="tagH"
                     /locus_tag="P6L01_001770"
     CDS             7529..8323
                     /gene="tagH"
                     /locus_tag="P6L01_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter
                     ATP-binding subunit TagH"
                     /protein_id="HDP3030837.1"
                     /translation="MNVSVNIKNVTKEYRIYRTNKERMKDALIPKHKNKTFFALDDIS
                     LKAYEGDVIGLVGINGSGKSTLSNIIGGSLSPTVGKVDRNGEVSVIAISAGLSGQLTG
                     IENIEFKMLCMGFKRKEIKAMTPKIIEFSELGEFIYQPVKKYSSGMRAKLGFSINITV
                     NPDILVIDEALSVGDQTFAQKCLDKIYEFKEQNKTIFFVSHNLGQVRQFCTKIAWIEG
                     GKLKDYGELDDVLPKYEAFLNDFKKKSKAEQKEFRNKLDEARFVIK"
BASE COUNT         2827 a         1442 c         1233 g         2852 t
ORIGIN      
        1 ttattaacat ctatatccat caatgtgcca cactttaatt tcctcgatgg tatatgtgta
       61 tttgctccaa gcttacttat atataaaatt aatctgttaa tttttgagac actacaaagt
      121 ttttatactt cgcatgtgat gccatcagtt ctgaatgcgt acctttacct gttacctgtc
      181 ctttgtctaa gaatataatt tgaccggctt ttttaattgt agacaaacga tgcgcaatga
      241 caatcgttgt tctaccttcc atcaatgttt ctaaagcttc ttgaattttc aattcacttt
      301 cactatcgag attagctgtt gcttcatcaa gtaacaaaat atcaggattt ttaacaaaac
      361 ttctagcaat atcaatacgt tgacgttgtc cgccagacag tttcaatcct cgttcaccta
      421 caagcgtgtc atatccttca tcaaattgca tgataaaatc atgacagttc gctaatttag
      481 cataattaat aagttcttca tctgaaacat gacgattaat tccgtataaa atattgtctc
      541 taattgttcc actcatcatc gaatttgatt gcataacata tccaattttg cgtcgccact
      601 tagataacgg gatatcatag acactttcaa ggccatattt aatatcacct gactcaattt
      661 catacatacg ttctatcaga ttaaatatcg tacttttacc agacccagaa ggacctacaa
      721 aagcactcac ttgaccttgt gggatttgga acgacacatc atctaatatt ttcttcacat
      781 catatttaaa gtctacatgt tcaaatgaca atacaccgtc atcaattaat acattttcag
      841 aatcttcaag agcttctgtc ggttcaatag gttcttgcat gatttcgtat attctactac
      901 ttgcaccgac tgccttttta taatctgtaa ctaacgtgga aagattgatt aaaggcatag
      961 ataactgaat aacataaaat atcattgcaa ttaatgtacc tgcagtgatt gcaccagtcg
     1021 caatttctaa tgcaccaaaa cctaaaataa ttgcaattgt tagcaacata actatacctg
     1081 aaattggttg tacaactgcc gcaattttag cctgttttaa acctaattta tatatttcat
     1141 tcaaattttt atgtgcatta tctaattcaa gacgctctgt atttgatatt ttaacaagac
     1201 gcatttcagt taggacacgc cctaacaaac cactgaagtt tgcaatttca gattgtgtac
     1261 ttgtcgatat cttttgcata atacgaccta gaggaatcat aattaaaacg aatatcggta
     1321 tcgttataaa tgttaataat gtcattttcc aatctaaaat aaataacatg attagtgacc
     1381 caactaatgt aacgattgat ggtaataagt taggtagctt ttgtgaaata aattcattta
     1441 tcactttcgt atcgtcagtt aatcgactca ttaattgacc actttcattt ttgtcaaaga
     1501 atggcatttt taattgtatg atatgctccc ataaaactga gcgtatcgca taaataatct
     1561 tttcaccaat tttacttaat aaatataaac ctaatccgct taataaagca ttgatgacaa
     1621 agataccacc aaataatgcg attagattcc aattgatatg gctcacggaa aatttatcta
     1681 caattcgtcc agtaaacagt ggcactaata gtccacttaa gctccctagt gatgaaatag
     1741 tgatagctgc aacgataaga cccactggcc atgatagttt tttaaataag aaaaacaatg
     1801 gattttctcg tttcatagtt ttacctcttc tgtttgaaat ttatagttaa ttcatttaga
     1861 tagatacatg catttctatc atatactttc tcccaacaaa actttaaaaa tcaaataaag
     1921 tgttgcccca aaataccgct cataacattt atatgataga atatttctat tgcatttttt
     1981 gtaataactt ctcaaagtct ttatattaaa acatgaaaat taatcgcttt tgagtaagtt
     2041 tgctcttcgt acaaacatta taacaccttt agctacacac gtaatatatt tgcattattt
     2101 ttcaaattaa ctgcagaaaa ctttattttc aacaatttaa ttaaggtgaa gtgaattagc
     2161 aatgatttcc gtaagtagga ataaccattg cttttaggga atatttaaga attggaaaag
     2221 ggaagattaa cgctttatga aaaatttaat atctattatc atcattttat gtttaacatt
     2281 aagtattatg acaccatatg cacaagctac taacagtgac gtaacccctg tacaagcagc
     2341 aaatcaatat ggttatgcag gtttgtcggc tgcatacgaa ccgacgagtg ctgttaatgt
     2401 aagtcaaact ggacaattac tgtatcaata caatatcgat actaagtgga atccagcgtc
     2461 tatgactaaa ttaatgacaa tgtacttaac attggaagct gtaaataagg ggcagctttc
     2521 acttgatgac acagtcacaa tgacgaacaa agaatatatt atgtctacac tacctgagtt
     2581 gagtaatacg aaactatatc ctggacaagt atggacaatc gcagacctat tacaaattac
     2641 agtatctaat tctagtaatg ccgcggcatt aattttagct aagaaggtat caaaaaacac
     2701 cagcgatttc gttgatttaa tgaataacaa agctaaagct atcggaatga aaaatacaca
     2761 tttcgtcaat ccaacgggtg ctgaaaattc aagattacgt tcatttgcac caacaaagta
     2821 taaagaccaa gaacgtactg taacgactgc tagagactat gccattttag atttacacgt
     2881 gattaaagag acacctaaaa tattagactt tacaaagcag ttagcaccaa caacgcatgc
     2941 agttacgtat tacacattca acttttcatt ggaaggtgca aaaatgagtt tgcctggtac
     3001 agatggttta aaaactggat caagtgatac agcaaattac aaccatacga ttactaccaa
     3061 acgaggtaaa tttagaatta atcaagttat catgggtgca ggagactata aaaaccttgg
     3121 tggcgagaag caacgtaata tgatggggaa tgcattaatg gaacgctcat ttgatcagta
     3181 taaatatgta aaaatattgt ctaaaggtga gcaaaggata aatggtaaga aatattatgt
     3241 tgaaaatgat ctttacgatg ttttaccaag tgattttagt aaaaaagatt ataaacttgt
     3301 agtcgaagat ggtaaagtac acgcggacta tccaagagaa tttattaata aagattatgg
     3361 acctccaact gtagaagttc atcagccaat tattcaaaag gcaaatactg ttgctaaaag
     3421 tatgtgggaa gaacatccat tattcactat cattggtggt gcatgccttg tcgctggatt
     3481 agcacttatt gttcatatga taatcaatcg tttatttaga aaaagaaaat aaaacatact
     3541 aaaaacggac aagttgcaca ttataaagct gcgaaacttg tccgttttat atttatttta
     3601 taataaccta agttaattta tcgtttagta tcgatagttc tatataattt atttagcatc
     3661 tttaccatat aattcttgtt tgattttagt cgtcgaaatg ccttctgtac gttttaaata
     3721 aatgacttca catttatcct ttaagaagtc gaattcacct tcccagtcat gtcccataac
     3781 aaaaacatct acatcaaatt tttcgacatc gtcttctttt tgtccccagc ccttttctgg
     3841 aatgactaaa tcgacatagc gtattgattc aagcatcatt tttcgttgtt cataatcata
     3901 ataagatttt ttatgtttaa tttgattaaa ttcatctgtt gataatgcta ctattaaata
     3961 atcgcccatc tctcttgcac gacgaagcaa ttcgatatga ccatagtgaa gtaagtcata
     4021 tgtgccatat gttattacac gtttcattaa gttatcctcc tcatattgtc acatcatagg
     4081 attgcagttt atggtcatca atatacacgt tatcgtttta acattgattt tgttcgatgt
     4141 gcaaactttc tccctttaag ttgtatcaag aattttttat aagtcggcct attaatgccc
     4201 acttgaatta atgtttgtcg ccacaattcg tatatttctc tatcccaatt ctcaccaata
     4261 aattcaactg tatttataat gtcaaacatg ttttgcgata cataatcaga atgctgcata
     4321 gacataatat agtcttttac gagttgaatt gtcgtgtgcg ttactgctgg gtgttcattc
     4381 atataatatt ttaatgaatt attgaataaa atacgatcaa ccatatcact atataatagc
     4441 ctttgttcag gtaatagtaa catttccatg actcgttgtc ttattttcat aatttctatg
     4501 atatatctat taaaatcttc atcgttactt gtagcatgtg tatctatatc gttatctccg
     4561 acaatcatct gtgaaactaa atgtatatcc gtagcttttt gcatcgcctt gacaagtatt
     4621 gcgtgattgt atgtgttagc taaagtttcg tcacactgta attcagcaaa tttagcactg
     4681 aataactttc cgtcaaaaga catcattgat aacaaatctg gttgttccgt taatgtaacg
     4741 attcgattat ctttaataaa ctcatcagca cttttcaatt tccattcacc atggtaattg
     4801 taagctcgct gccctattac catatcaata ttatcattat ttttgaaagc tgcagttaac
     4861 ctatctaatt tacctggcaa tacgattgat gttggattta tgaccataaa tgcgtcacta
     4921 tcatgtggat ctgtcatttt gatatccatc aaacctttat taatacacgt agctatcgaa
     4981 gcattttgta attgtattaa tgaaatatgc ttgagttctc tttgtaaccg ttcaatcata
     5041 ggaattgttt gatcagtaga accaccatca atacaaagga ttctatagtg ttctttactc
     5101 tcaatagata ttaacaattg tcgaattgtt gcctcattat tacatgtagg tatgattatc
     5161 gtaagcctca ttttgtcacc atcttatcta tatattctgt gagctgatgt aaacttttat
     5221 cagtattata cttatgccaa tctttaaata acggacttaa tagatgttct ttttcttgta
     5281 tcgtcattat taaatcttct tcagtataca ctttgtagct atccggtatt gctttgtaaa
     5341 attgattcag gcctctcact ttatcatatg ttccttcatc atacacataa aatatagttg
     5401 gaatatctaa caagctagct tctattggca gcgaactata gtcgctaata attatatctg
     5461 acattagcat taatgtagac gtgtcgattg aagatacgtc atcaatgtct gaatcttcaa
     5521 ttgatggatg taatttatta atcagtgtat atcctggtaa acatttttca aaataagctt
     5581 tatcaatagc cctattatct gctttatctt ctctatatgt tggtacatat aataccaact
     5641 tatttgtaat tccatattta tcctttaact ctgccttaac cgttgctcta tcagctgtgt
     5701 aatatttatt aattctcgga agcccaaaat acagcatttg ctcttctgtt gcacctaaag
     5761 actgtttaaa acattgtgac atttgttcac aacccactaa gtaaaaatcc gtcgcttgat
     5821 aaactttacg gtactgctga accattgcct tgtcagacac atcgacttga tgatctgtta
     5881 atccaaagtt ttttaatgca ccacttgcat gccacgtttg aacaatgtgt tgattagaag
     5941 tcttattata tccacctagt aataggtaat aattatcgat aataatcatc tgcgcgcttt
     6001 tcaaagcctt aatttgtttt accaatgttc gattagtcat ttctatcaca tcaacatcgt
     6061 cgctaagttc agataaataa ggcgcttgtt ttggtgttgt taaaacagtt ttctgatacg
     6121 acgaattatt taatgctttg atgataggct taatatcttc tggaaaagtc atcataaata
     6181 cgatatgcgg tttatcaatc acttgaggcg taatcatttt agaaagtatt cgaactacca
     6241 aatgataaaa tttctttatt aaaacgttca taatacacca acttaatatg ttatttactt
     6301 aaattataaa caaaaatgaa ccccacttcc atttattaat ggttagcggg gtttcgtcat
     6361 ataaatatat tacaagaagt ctgcaaattg atctctatat ttcatgtgta agtacgcacc
     6421 aattgcaaag aaaatggcaa caataccgaa attgtataac attaatttcc aatgatccat
     6481 gaaataccat tcgtgatata aaattgctgc acggtatgat tcagcaataa agtaaactgg
     6541 attatatttc atcatttcat gaattaaacc actgatacca tggttctttg gtagccacaa
     6601 aattggtgaa aagtaaaata atattcttaa tattgcttgc attaacattt gtgtatctct
     6661 aactaacaca ccgagtgttg atgttaataa cgtcaccgag gcagttaaga aaaaacaaaa
     6721 cggtacatat atcaataatt gaatgatatg tattgatgga taaataccag taaacataca
     6781 tgcaattatc acaagtaaaa gtaagcctaa atgtccataa aatctacttg tcacaatata
     6841 tgtcggtatt atcgataacg ggaagttcat tttcgatact tgattaaact tttgtgtaat
     6901 tgctttagta ccttctaaaa taccttggtt gatgaagaac cacatactga taccaaccaa
     6961 taaccaataa acaaaaggta caccatgaat tggtgcatta cttcttattc ctaatccaaa
     7021 aaccatccag taaaccataa tttgcataac agggttaatt aattcccaag ccacacctaa
     7081 atagttacta tgattgataa ttttaacttg aaactgagcc agtctttgaa ttaaataaaa
     7141 gttctttaca tgttctttaa aaactgttcc tattgctgac attccattaa accacacttt
     7201 caaatgttta actatttctc tacttaacta aatagtatta taataattgt tgtaaatact
     7261 atcactatac atggatgcta tcaaaattat tgtctagttc tttaaaatat tagtttatta
     7321 caaatacatt atagtataca atcatgtaag ttgaaataag tttagttttt aaatatcatt
     7381 gttatcattg atgattaaca ttttgtgtca aaacaccaac tctgataata acaaaatctt
     7441 ctatacactt tacaacaggt tttaaaattt aacaactgtt gagtagtata ttataatcta
     7501 gataaatgtg aataaggaag gtctacaaat gaacgtttcg gtaaacatta aaaatgtaac
     7561 aaaagaatat cgtatttatc gtacaaataa agaacgtatg aaagatgcgc tcattcccaa
     7621 acataaaaac aaaacatttt tcgctttaga tgacattagt ttaaaagcat atgaaggtga
     7681 cgtcataggg cttgttggca tcaatggttc cggcaaatca acgttgagca atatcattgg
     7741 cggttctttg tcgcctactg ttggcaaagt ggatcgtaat ggtgaagtca gcgttatcgc
     7801 aattagtgct ggcttgagtg gacaacttac agggattgaa aatatcgaat ttaaaatgtt
     7861 atgtatgggc tttaagcgaa aagaaattaa agcgatgaca cctaagatta ttgaatttag
     7921 tgaacttggt gagtttattt atcaaccagt taaaaagtat tcaagtggta tgcgtgcaaa
     7981 acttggtttt tcaattaata tcacagttaa tccagatatc ttagtcattg acgaagcttt
     8041 atctgtaggt gaccaaactt ttgcacaaaa atgtttagat aaaatttacg agtttaaaga
     8101 gcaaaacaaa accatctttt tcgttagtca taacttaggc caagtgagac aattttgtac
     8161 taagattgct tggattgaag gcggaaagtt aaaagattac ggtgaacttg atgatgtatt
     8221 acctaaatat gaagctttcc ttaacgattt taaaaagaaa tccaaagccg aacaaaaaga
     8281 atttagaaac aaactcgatg aggcccgctt cgttattaaa taaaccgaaa aaaccgagaa
     8341 tctccattta agga
//
LOCUS       DAOBBH010000109         8321 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.109, whole genome shotgun sequence.
ACCESSION   DAOBBH010000109 DAOBBH010000000
VERSION     DAOBBH010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8321
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.109"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(75..259)
                     /locus_tag="P6L01_001771"
                     /pseudo
     CDS             complement(75..259)
                     /locus_tag="P6L01_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(348..1412)
                     /locus_tag="P6L01_001772"
     CDS             complement(348..1412)
                     /locus_tag="P6L01_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HDP3030838.1"
                     /translation="MLTVDQVKELVGEIKDPIIDVPLKETEGIVEVSIKEEKEHVSVK
                     LAMAQLGGAPQLDLQMAVVNALKENGAKTVGIRFETLPEEKVNQFKPKEENKPKTIEG
                     LLSQNNPVEFIAIASGKGGVGKSTVAVNLAVALAREGKKVGLVDADIYGFSVPDMMGI
                     DEKPGIKGKEVIPVERHGVKVISMAFFVEENAPVIWRGPMLGKMLTNFFTEVKWGDIE
                     YLILDLPPGTGDVALDVHTMLPSSKEIIVTTPHPTAAFVAARAGAMAKHTDHSILGVI
                     ENMSYFESKETGNKEYVFGKGGGTKLADELNTQLLGELPLEQPSWNPKDFAPSIYQSD
                     DRLGKIYSSIAQKVIAATNK"
     gene            complement(1560..3002)
                     /gene="lmrS"
                     /locus_tag="P6L01_001773"
     CDS             complement(1560..3002)
                     /gene="lmrS"
                     /locus_tag="P6L01_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter LmrS"
                     /protein_id="HDP3030839.1"
                     /translation="MAKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELN
                     INESTSQWLVTGFMLVNGVMIPLTAYLMDRIKTRPLYLAAMGTFLLGSIVAALAPNFG
                     VLMLARVIQAMGAGVLMPLMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTVTGLI
                     IDQASWRVPFIIIVGIALVAFVFGLVSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAF
                     SSAGDLGFTSPIVIGALIISMVIIYLFIRRQFNITNVLLNLRVFKNRTFALCTISSMI
                     IMMSMVGPALLIPLYVQNSLSLSALLSGLVIMPGAIINGIMSVFTGKFYDKYGPRPLI
                     YTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEIS
                     HGTAIMNFGRVMAGSLGTALMVTLMSFGAKIFLSTSPSHLTATEIKQQSIAIGVDISF
                     AFVAVLVMAAYVIALFIREPKEIESNRRKF"
     gene            complement(3325..3798)
                     /gene="sepA"
                     /locus_tag="P6L01_001774"
     CDS             complement(3325..3798)
                     /gene="sepA"
                     /locus_tag="P6L01_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter SepA"
                     /protein_id="HDP3030840.1"
                     /translation="MIVNYLKHKFYNLLTTMIVLFIFVLSGAIFLTFLGFGLYGLSRI
                     LIYFRLGDFTYNRSMYDNLLYYGSYIIFGYFIIFAVEHLMDYFRKMLPENAYFRGATF
                     HLISYTVATTLFYFIIHLNYVYINIDFWVIMVIIGFLYVCKLQFYPESKNLNNRK"
     gene            complement(3897..5240)
                     /locus_tag="P6L01_001775"
     CDS             complement(3897..5240)
                     /locus_tag="P6L01_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDP3030841.1"
                     /translation="MRLKSIITVIALILIMFMSAIESSIISLALPTIKQDLNAGNLIS
                     LIFTAYFIALVIANPIVGELLSRFKIIYVAIAGLLLFSIGSFMCGLSTNFTMLIISRV
                     IQGFGSGVLMSLSQIVPKLAFEIPLRYKIMGIVGSVWGISSIIGPLLGGGILEFATWH
                     WLFYINIPIAIIAIILVIWTFHFPEEETVAKSKFDTKGLTLFYVFIGLIMFALLNQQL
                     LLLNFLSFILAIVVAMCLFKVEKHVSSPFLPVVEFNRSITLVFITDLLTAICLMGFNL
                     YIPVYLQEQLGLSPLQSGLVIFPLSVAWITLNFNLHRIEAKLSRKVIYLLSFTLLLVS
                     SIIISFGIKLPVLIAFVLILAGLSFGYIYTKDSVIVQEETSPLQMKKMMSFYGLTKNL
                     GASIGSTIMGYLYAIQSGIFGPNLHNVLSAVAVISIGLIVLWVVFFKEQSSQSKE"
     gene            complement(5417..6100)
                     /locus_tag="P6L01_001776"
     CDS             complement(5417..6100)
                     /locus_tag="P6L01_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDP3030842.1"
                     /translation="MSKSTKKSNDTIVETFKDIIPLSFGEEIGNAASHGVAALLTLLV
                     LPYAAVHSYIAHGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMI
                     YVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMG
                     WVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLL
                     HLIAILYFM"
     gene            complement(6119..7306)
                     /locus_tag="P6L01_001777"
     CDS             complement(6119..7306)
                     /locus_tag="P6L01_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HDP3030843.1"
                     /translation="MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAK
                     LYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEEQGLQAIKEGQFAVLLMAGG
                     QGTRLGYKGPKGSFEIEGVSLFELQANQLKTLNHQSGHTIQWYIMTSDINHEETLAYF
                     EAHSYFGYDQEAIHFFKQDNIVALSEEGKLILNQQGRIMETPNGNGGVFKSLDKAGYL
                     EEMSNNGVKYIFLNNIDNVLVKVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNV
                     DCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDEN
                     FGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNKEGKDSVATATEDLRRM
                     GLI"
     gene            complement(7350..7865)
                     /locus_tag="P6L01_001778"
     CDS             complement(7350..7865)
                     /locus_tag="P6L01_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDP3030844.1"
                     /translation="MTGKTHASCGMLVGALTTQYFQTDIFSSVTVIILATLASLLPDI
                     CHTQSKIGRRFKVISFFVRLIFGHRTFTHSILFIAIIAFLLQIIQTPNYYMAAIIIGL
                     VSHVILDMITPRGVKLFYPLPFNVKTPIQFKTGGLVDLSLATALMVGTVYVLFQPFIN
                     DIISNWNTKFL"
     gene            8042..8302
                     /locus_tag="P6L01_001779"
     CDS             8042..8302
                     /locus_tag="P6L01_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030845.1"
                     /translation="MAMTVKKDNNEVRIQWRVADIKIPTSEIKNITQDQDIHAVPKLD
                     SKDVSRIGSTFGKTNRVIIDTEDHEYIIYTQNDQKVYNELTK"
BASE COUNT         2964 a         1530 c         1075 g         2752 t
ORIGIN      
        1 atgttcaatc tcttttattc tacttcattt gtttcttgaa gtcaataact ttttgaacga
       61 ttactttatt tattctatat tgtttttata gttatttcaa tggtaagttt tacacttttg
      121 aaattcttct ttaaaaacaa ctgcgtcgtt tttgacgact ttatcatatt atcaactttg
      181 gaatttaaag tcaataactt ttttaaaact ttttgtgttc acaacccgct tctttttcaa
      241 cgcgtttatt gcttaacaca agaacttatt ttaccagcat tccaaaacaa atcaacataa
      301 aaacgtacaa aataaaagta attttgtacg tttagcatat attataccta tttatttgta
      361 gcagctataa ctttttgtgc aatcgagcta taaattttac ctagacgatc atctgattga
      421 tatattgacg gtgcaaaatc ttttggattc caagatggtt gctctaaagg taattcccca
      481 agtaattgag tattaagttc atcagctaac ttagtaccgc cacctttgcc aaagacatat
      541 tctttattac ccgtctcttt actttcaaaa taactcatgt tttcaattac gccaagaata
      601 gaatgatccg tatgttttgc catcgcacct gcgcgagctg caacaaatgc tgctgtagga
      661 tgaggtgtcg ttacaataat ttccttactt gaaggtaaca tcgtatgaac atctaaagct
      721 acatctcctg ttccaggtgg aagatcgagt attaaatatt caatgtctcc ccatttaact
      781 tctgtaaaga aattcgtcaa cattttacct aacattggcc ctctccatat aactggcgca
      841 ttttcttcca caaaaaaggc cattgatata actttaacgc catgacgttc aactggaatt
      901 acttccttcc ctttaattcc aggcttttca tcaataccca tcatatctgg tacactaaat
      961 ccatatatat cggcatctac taatccgact tttttccctt cacgagctaa ggcaacggct
     1021 aaatttactg caacagtaga tttaccgaca ccacctttac cggaggctat tgcaataaat
     1081 tcgactggat tattttgaga taataggcct tctatcgttt taggtttatt ttcttctttt
     1141 ggtttaaatt gatttacttt ttcttccggc aatgtttcaa atcgtatacc gaccgttttc
     1201 gcaccgtttt cttttaatgc attaacaaca gccatctgta aatctaattg cggtgcacca
     1261 cctaattgtg ccattgctaa tttaacactc acatgttctt tttcttcctt aatagaaact
     1321 tcaacaatac cttctgtttc ttttaaaggc acatctataa taggatcttt aatttctcct
     1381 accaattctt tcacttgatc taccgttaac aaagcaaatc ccccttaatc attttaagac
     1441 atctacatca tagcaaataa caatttttgc ttagtctatc actaaaagta taaaactttt
     1501 tattaaataa aaaaagcagt tagcctatta ttttaaatag accaactact ataattattt
     1561 taaaatttcc ttctattact ttctatttct ttaggttctc ttataaaaag tgctatcaca
     1621 taagctgcca taacaagcac agctacaaaa gcaaatgaga tatccacccc tatagcaatg
     1681 gattgctgtt taatttcagt tgcagttaaa tgcgatggcg atgtagataa aaatattttt
     1741 gcaccaaaac tcattaatgt aaccattaaa gctgtgccta gtgaaccagc cattacacga
     1801 ccaaagttca taatagccgt gccatgtgag atttcttcat ttctcaaaga attaattcct
     1861 gtagtattta tcggcatcat gagtaaagaa actgaaaaca ttctaattgc atatacgact
     1921 attaaatacg tataagatgt gtctgtatgc aagaaacaca acataattgt agtaattgtt
     1981 aaaattgtaa aaccagtata aatcaatggt ctaggaccat acttatcata aaatttacct
     2041 gtaaaaactg acataatacc atttattatt gcaccaggca tgataacaag tcctgataac
     2101 aaggcagata aagataaact attttgaaca tatagcggta taagcagcgc aggtccaacc
     2161 attgacatca ttataatcat tgaactaatc gtacataatg caaatgttct atttttaaaa
     2221 acccttaaat ttaaaagtac attagtaata ttaaattgtc tacgtataaa taaatagata
     2281 ataaccatac ttattatcaa cgcacctatt actattggac ttgtaaatcc taaatctcct
     2341 gcactactaa atgcgtatag cattaacccg aatccaatag ttgaatacat tactgagcgc
     2401 ttatctaatt tcgtatattt cacttcattg taacttgaga ttgaaactaa gccgaaaaca
     2461 aaggcaacta aagcaattcc tacaattata ataaatggta ctcgccaact cgcttgatca
     2521 ataattaatc ctgtaactgt agggcctatt gctggtgcaa attgaattac taaacctgct
     2581 agtcccattg caaaacctcg atgttcttta ctgaacaatg taaataacgt aaattgcatt
     2641 aagggcataa gtacgcctgc acccatcgct tgaattacac gtgctaacat taaaactcca
     2701 aaattcggag ctaaggctgc aacaatagaa cctaataaaa atgtccccat cgccgctaag
     2761 tataaaggtc tagttttaat tctatccatt agatatgccg tcagaggtat catgacgcca
     2821 ttaacaagca taaacccagt aactagccat tgcgatgtac tttcattaat atttaattct
     2881 ctcattatac taggtaacgc tgtatttaat aatgtttgat ttaaaatagc tacaaacgca
     2941 ctaatcaaca taacagcaac tatgaagttt cgtctccggg ttgttaattc aactttagcc
     3001 atcataattc ctccttttta aaacagatat acacttgtcc ctttaataca tatagccatt
     3061 aacataatgg gagtagtact caaatgataa agaagcacta cataataaat cattattggt
     3121 tcttacacat taggcacagc taatgtgtac ttaaaaaact aacaatataa cattgtaggg
     3181 cctaataatt tattttattt ctcagactca tacaatggtt atttaactta tttaccacaa
     3241 attgagcact aagactcact attatcgatt cttctattga acaaaaagaa gttggaacac
     3301 atatgtgccc tcaacttcag atatctattt tctattattt aaatttttac tttcagggta
     3361 aaattgaagc ttacaaacat ataagaaccc tataataacc atgatgaccc aaaaatcgat
     3421 attgatataa acataattta aatgaatgat gaaataaaat aacgttgttg caactgtgta
     3481 agaaatcaaa tgaaaagtcg cgcctctaaa atatgcattt tcaggcaaca tttttctaaa
     3541 ataatccatt aaatgctcga ctgcaaatat gatgaagtac ccaaagatta tataactgcc
     3601 ataatataat aggttatcgt acatacttct attatatgta aagtcaccca acctaaaata
     3661 aataagtatt ctacttaatc catataaacc aaaccctaga aatgttaaaa aaatcgcacc
     3721 agaaagtaca aaaataaaga gaacaatcat tgtagttaac aagttataaa acttatgttt
     3781 aagataattc acaatcattg gcctccttat taataaaata ttgaacgatt tatctagtat
     3841 ctaggaaatt tattatttct tactttaatt acactacatc aattttaatc ataattctat
     3901 tcttttgatt gagatgactg ttctttaaaa aatacgaccc ataaaacaat aagcccaatg
     3961 ctaattacag caacagcact taacacattg tgtaagtttg gaccaaagat tcctgattgt
     4021 atcgcataaa gatatcccat tattgttgaa cctattgatg ccccaagatt tttagttaat
     4081 ccataaaatg acatcatttt cttcatttgt aatgggctag tttcctcttg gacaattaca
     4141 ctatctttcg tataaatata tccaaaactt aatcctgcca aaattaacac aaatgctata
     4201 agtaccggca atttaatacc aaatgaaatg ataatactac ttactagtaa caatgtaaat
     4261 gatagtaagt aaataacttt ccttgatagt tttgcttcaa ttcgatgtaa attaaaattc
     4321 aatgtaatcc aagctacaga taaaggaaaa ataaccaatc cactttgcaa tggagataat
     4381 cctagttgtt cttgaaggta gactggaata tataaattga atcccattaa acaaatagct
     4441 gttagaaggt ccgttataaa aactaaagta atcgaacgat taaattcaac cacaggtaaa
     4501 aatggtgaag aaacatgttt ttctacttta aataaacaca ttgccacaac gatagctaag
     4561 ataaaactca agaaatttaa aagtaatagc tgttggttta ataaagcaaa cataatcaat
     4621 ccaataaata cataaaatag cgtgagccct ttagtatcaa attttgattt agcaactgtt
     4681 tcttcctctg gaaaatgaaa tgtccatata actaatataa tggcaatgat cgcaatcggt
     4741 atatttatgt agaataacca atgccatgtt gcaaattcta atataccacc acctagaagc
     4801 ggtccaatga tactcgaaat accccaaaca ctgccaacta tgcccataat tttatatcgt
     4861 aacggaattt caaatgctaa ctttggaaca atttgcgata atgacatcaa tacacctgat
     4921 ccaaaacctt gtatcacgcg tgatataatt agcatagtaa aattagtact taaaccacac
     4981 ataaaactac ctatactaaa taaaagtaat cctgcaattg ccacataaat aattttaaat
     5041 ctcgaaagta attctcccac tataggattg gcaattacta atgcgataaa atacgctgta
     5101 aaaattaatg aaattaaatt tcctgcattt aaatcttgtt taattgtagg taatgcaagc
     5161 gaaataatag atgattcaat cgctgacata aacataatta atattaaagc tataacagtt
     5221 atgattgact ttaatcgcat gaaatacctc cttctatata tttatttata agattttatt
     5281 tacaaataat tggttttcat atgtataaac acttttgact ttcaaatctt aacgataatt
     5341 ctattacaat acaatcccct attagaatga tttatgtaat aaaaaaagcg gagtttcccc
     5401 cagcttttct aaacgactac ataaaatata agatcgcaat taaatgcaat agtgatgcta
     5461 ttacaataaa aatatgccaa atcatatgaa aatatggtct attcttttgt gcataaaacc
     5521 atgcaccaat tgtataagac acaccaccta agaaaatgaa taatatgaat atccatgatg
     5581 tgcgaataaa aataattggt aacaagataa tacctaccca gcccatcact aaatagacaa
     5641 ttaagcttaa cttttggtta actttgacag ctatggattt atataaaatc ccccaaagtg
     5701 tcgttcccca taataaaata aatactgtcc aacctatcca accacctaca actgttaaca
     5761 aaataggcgt atatgtacca gaaattgcta cgtaaatcat actgtgatcg ataatcctta
     5821 aaatatattt atgaggtgtt tcattttgca tagaatgata aatagtagat gaaataaaca
     5881 tcataaagat agaaataaca taaatagaca tactcacaga cgcaagcgta ccatgtgcaa
     5941 tataactgtg cacagccgca tagggtaaca ctaataatgt taacaaagct gcaacaccat
     6001 gagatgccgc attaccaatt tcttctccaa atgataaagg gatgatatcc ttaaatgttt
     6061 ctacgattgt gtcattactt tttttagttg atttggacat catagtcacc gcctatcatt
     6121 aaattaaacc cattctacgt aaatcttctg ttgcagttgc aacactatct ttaccttctt
     6181 tatttttaag aggtgaaaat tcttcttctc taggaacttg taacgtgaca aaactagttc
     6241 cataagtaaa tatgtcaaaa tagaacaatt cgaattttaa agttggttgt tcaataaccc
     6301 caaagttctc atctaattgt ttcaaattct taatagctaa atgataaggt aactcacgat
     6361 ttacagcgtt taaaataaaa ccaagtttaa atgcatgaat accgatatta gcattgttaa
     6421 attgatttgc aacttctgga tctaattctg aatactccag tactgtatct ttacaatcga
     6481 cattaactaa acgtccaaca ctttctcctg gttttggttg aatcgtttta gaagtaatat
     6541 cgtaatcatg ttctaccgta aatcctgcaa atagtggatc caacactttt actaaaacat
     6601 tgtcgatatt attcaagaaa atatacttaa cgccattatt agacatttct tctaagtatc
     6661 ctgccttgtc taaagattta aacacgccac cattaccatt cggcgtttcc ataatacgac
     6721 cctgttgatt taaaattaac ttcccttctt cactaagcgc cacaatatta tcttgtttaa
     6781 agaaatggat tgcttcttga tcatatccaa aataactatg tgcttcgaaa taagctaaag
     6841 tttcttcatg attgatatca cttgtcataa tataccattg aatcgtatgt ccagattgat
     6901 gattcaatgt ttttaattgg ttagcttgaa gttcaaataa acttacacct tcaatttcaa
     6961 acgaaccttt agggcccttg taaccaagtc ttgttccttg accacctgcc atcaaaagta
     7021 ctgcaaattg tccttcttta atagcttgaa gtccttgttc ctctaaacgc ttaatctcat
     7081 catcactcat ttgtgattta atatcatatt tcacttcaga aactgcagat acatcatcta
     7141 ttgttttctt attgatgtat aaatcgttat ataatttggc aataaaatct aaatctaatg
     7201 acgctacctt ctcttcaagt gcttcttttt cattgttact cattattttt tcatattcac
     7261 acaaatgatc ttgtttatat ttagctaatt gatttttatc tagcatttta tgcctcctgc
     7321 tgagtttgta acctttaata atttattttc tataaaaact tagtattcca gttgcttatt
     7381 atatcattga tgaaaggctg aaataaaaca taaactgttc cgaccattaa cgctgtagct
     7441 aaagataagt ctacaagtcc acctgtttta aattgaatcg gtgtcttcac attaaacggt
     7501 aacggataaa ataatttcac gcctcttggt gttatcatat ctagtataac atgtgacact
     7561 aacccaatta taattgctgc catataataa ttaggcgtct gaataatttg caataaaaat
     7621 gctatgattg cgataaataa tattgaatgc gtaaaggttc gatgtccaaa gatcaatctc
     7681 acaaaaaaac taattacctt aaaccttctg ccaatcttac tttgagtgtg acatatatcg
     7741 ggtaataagc tagctagagt tgctagaatg ataaccgtaa ccgacgaaaa aatatccgtt
     7801 tgaaaatatt gtgttgttag cgctccaacg agcatgccac atgaagcatg tgttttacct
     7861 gtcatatttg ttctccttta atactcacat tttaccacat ccctaacaaa aacacgaaca
     7921 tattttcggg ttaaaattca ttagtatgac acaatttaaa aaagtatcac ataactcttg
     7981 aaaacgatta caaaatcgtt tatgatgtat ttacaaaata tttaaaggat gtgtttgaat
     8041 aatggcaatg acagtaaaga aagataataa tgaagtgcgt attcaatgga gagttgctga
     8101 tatcaaaatt cctacaagtg aaattaaaaa tattacacaa gaccaagata ttcatgcagt
     8161 tcctaaatta gacagcaaag atgtatctag aatcggctca acgtttggta aaacgaatcg
     8221 cgttattatc gatactgaag accacgaata cattatttat actcaaaatg atcaaaaagt
     8281 ttacaatgaa ttaactaaat aaattgtata aaaaaatcat t
//
LOCUS       DAOBBH010000110         8300 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.110, whole genome shotgun sequence.
ACCESSION   DAOBBH010000110 DAOBBH010000000
VERSION     DAOBBH010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8300
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.110"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(32..973)
                     /gene="yhaM"
                     /locus_tag="P6L01_001780"
     CDS             complement(32..973)
                     /gene="yhaM"
                     /locus_tag="P6L01_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM"
                     /protein_id="HDP3030846.1"
                     /translation="MRNIENLNPGDSVDHFFLVHKATQGVTAQGKDYMTLHLQDKSGE
                     IEAKFWTATKNDMATIKPEEIVHVKGDIINYRGNKQMKVNQIRLATTEDQLKTEQFVD
                     GAPLSPAEIQEEISHYLLDIENANLQRITRHLLKKYQERFYTYPAASSHHHNFASGLS
                     YHVLTMLRIAKSICDIYPLLNKSLLYSGIILHDIGKVRELSGPVATSYTVEGNLLGHI
                     SIASDEVVEAARELNIEGEEIMLLRHMILSHHGKLEYGSPKLPYLKEAEILCYIDNID
                     ARMNMFEKAYKKTDKGQFTDKIFGLENRRFYNPESLD"
     gene            complement(970..3906)
                     /locus_tag="P6L01_001781"
     CDS             complement(970..3906)
                     /locus_tag="P6L01_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDP3030847.1"
                     /translation="MIIKSLEIYGYGQFVQRKIEFNKNFTEIFGENEAGKSTIQAFIH
                     SILFGFPTKKSKEPRLEPRLGNQYGGKLVLILDDGLEIEVERIKGSAQGDVKVYLPNG
                     AVRDDAWLQKKLNYISKKTYQGIFSFDVLGLQDIHRNLNEKQLQDYLLQAGALGSTEF
                     TSMREVINRKKDELYKKSGKNPIINQQIEQLKQLESQIREEEAKLETYHRLVDDRDKS
                     SRRLENLKHNLNQLSKMHEEKQKEVALHDHSQEWKSLEQQLNIEPITFPEKGVDRYEK
                     ARAHKQSLERDIGLRNERLAQLKEEATQLEPVKQSDIDAFISLNQQENEIKNKEFELT
                     AIEKDIANKQRDKDELQANIGWSETHHDVDSSEAMKSYVSEQIKNKQEQAAYIKQLER
                     SLEENKIEDNAVHSELDSVEEKIVPEETFEKKKEYSQQVIELNEKENLYSKLKERFEI
                     EQQEKQKRQKLLRTTFILLTLVGIGLTAFSFISNNMLFGIIFAVLTLVFVIGIIMSKS
                     KEVDYSEAITDEIEEIKAQLAILDENYDLDFDLDEQYRIRDHWQQALKNKDILEEKRQ
                     YIEGRLNDAKGRHDELQSTVENVKDELYLSSKISNDLIVDSISTMANIKALDQHISDL
                     NQQRQQLVQELDTFYNHAEAVTKSQFVYFNKLSLFHDVQQWLKSAEDTNEKWRINAEN
                     TKLVTNELNHLNAQLEENNKEITALFDFINVGTEEDFYQHHEDYQTYTSNLSRFNDLT
                     KYLENQNYSYELSSSLSEKTTAQLEEEDHLLATQVDEYNEQYLEMQAQVSDLSAQINH
                     METDTTLANLRHEYHSLKNQLNDIAKDWASLSYLQSLVDEHIKQIKDKRLPQVINEAV
                     EILKHLTDGRYTMINYNEDSITVKHVNGQLYDPVELSQSTKELLYVALRISLIKVLRP
                     YYPFPLIVDDAFVHFDKKRTEKMLNYLRSLSEHYQVLYFTCVKDNIVPSKEVITLNKI
                     EEGGKR"
     gene            complement(3896..5092)
                     /locus_tag="P6L01_001782"
     CDS             complement(3896..5092)
                     /locus_tag="P6L01_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDP3030848.1"
                     /translation="MVKFIHCSDLHLDSPFKSKSHISPKIFEDVQKSAYESFKNIVDI
                     ALQQDVDFVIIAGDLFDSENRTLRAEIFLKQQFERLQNEQIFVYVCHGNHDPLSSKIS
                     SNWPDNVSVFSNKVETYEAITKSGETIYIHGFSYENRASYENKIDEYPSSQGQKGIHI
                     GVLHGTYSKSSVNERYTEFILEDLNSKLYHYWALGHIHERQQLSDMPVINYSGNIQGR
                     HFNEQGEKGCLLIEGDHLKLKTKFYPTQYIRFEEATIETDKTSKQGLYEVIQNFKEQV
                     REEGKAFYRLTLVINSETLISPQDLLQVEEMITDYEENENQFVYIDELKIQYAQNDES
                     PLVNEFSAELLVDQTVFDKAMSDLYLNPRASKFLDDYGTFDHTALVNRAEEILKAEMR
                     GEQNDN"
     gene            complement(5383..5481)
                     /locus_tag="P6L01_001783"
     CDS             complement(5383..5481)
                     /locus_tag="P6L01_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030849.1"
                     /translation="MSVGSLAVLTITEIAFPTEIFEKNQRTLQIKF"
     gene            complement(6020..6364)
                     /locus_tag="P6L01_001784"
     CDS             complement(6020..6364)
                     /locus_tag="P6L01_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HDP3030850.1"
                     /translation="MAVNLYDYANQLEQALRESEEYKAIKEAFANVKANEESKKLFDE
                     FRETQINFQQKQMQGEEIAEEDLQKAQEQAQAIEKDENISALMNAEQKMSQVFQEINQ
                     IIVKPLDEIYAD"
     gene            complement(6433..7557)
                     /locus_tag="P6L01_001785"
     CDS             complement(6433..7557)
                     /locus_tag="P6L01_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HDP3030851.1"
                     /translation="MNALFIIIFMIVVGAIIGGITNVIAIRMLFHPFKPYYIFKFRVP
                     FTPGLIPKRREEIATKIGQVIEEHLLTETLINEKLKSEQSQQAIESMIQQQLQKLTKD
                     QLSIKQITSQIDIDLEQVLQTNGNQYIESQLNNYYTKHQNQTIASLLPNQLVTFLDQH
                     VDNATDLLCDRARNYLSSAKGTQDINDMLDTFFHEKGKLIGMLQMFMTKESIADRIQQ
                     ELIRLTSHPKARTIVTSLITNEYQTFKDKPLNELLDASQFNEIAENLSVYVTTYASNQ
                     ANKPVVTLMPQFVDYLEGQLSSKLANLIIEKLSIHLSTIMKKVDLRGLIEEQINTFDL
                     DYIEKLIIEIANKELKLIMSLGFILGGIIGFFQGLVAIFV"
     gene            complement(7736..8200)
                     /locus_tag="P6L01_001786"
     CDS             complement(7736..8200)
                     /locus_tag="P6L01_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDP3030852.1"
                     /translation="MDRQSFTDLIQTKFKMVRIEAGYTQDTMAQTIGLSKKTLVQIEK
                     ERVLPNWTTCISICALFRDSEVLNSTFGCDPLEIVQTISRNHCAYPNHAPTSDIYWNN
                     IETRNGYILQSNKVSNIYRVLNPDNQPIFGTSKMREAETYFNRNAKEELVHI"
BASE COUNT         2496 a         1453 c         1145 g         3206 t
ORIGIN      
        1 gtatactcat ctgtccgata tcttttaatt cttaatcgag tgattcagga ttgtagaatc
       61 tacgattttc aagaccaaat attttatctg taaactgacc cttgtcagtt tttttataag
      121 ccttttcaaa catattcatt ctagcatcga tgttatcgat atagcataaa atttctgctt
      181 cttttaagta tggcagtttt ggcgaaccat actctaactt accatgatga gataaaatca
      241 tatgtcttaa caacatgatt tcttctcctt caatgttcaa ttcacgagct gcttcaacta
      301 cttcatcact cgcaatcgag atgtgtccta ataagttacc ttcgactgta tacgacgtcg
      361 caacaggacc actcaattct ctaactttac caatatcatg caaaataata ccactatata
      421 acaaactttt gtttaacaat ggataaatgt cacaaattga ttttgcaata cgtaacatcg
      481 tcaatacatg atagcttaag ccactcgcaa agttatgatg atgagaacta gcagctggat
      541 atgtgtaaaa tcgttcttga tattttttca ataaatgacg tgtgatacgt tgtaaattag
      601 cattttcaat atctagcaaa taatgagaaa tctcttcttg tatttctgcc ggtgataaag
      661 gtgcaccatc tacaaattgt tctgttttta attgatcttc agttgtcgct agtctaattt
      721 ggttgacttt catctgttta tttccgcgat agtttatgat gtcaccttta acatgtacaa
      781 tttcttcagg cttgattgtt gccatatcat tttttgtagc cgtccaaaat ttcgcttcaa
      841 tttcaccact tttatcttgc aaatgtaatg tcatataatc tttaccttgt gctgttacac
      901 cctgtgtagc tttatgcact aagaaaaagt gatcaactga atctccggga tttagattct
      961 ctatatttct catcgtttcc cgccttcctc tattttgttt aatgtaatca cttcttttga
     1021 tggaacaata ttatctttta cacatgtaaa gtatagtact tgatagtgtt ctgataatga
     1081 tcgtaaataa ttcaacattt tttcagtacg ctttttatca aaatgaacaa atgcatcatc
     1141 aacaattaat gggaacggat aatatggtct tagtacctta attaaactga tacgtaaagc
     1201 tacataaagt aattcttttg tagattgact tagttcaaca ggatcatata attgaccatt
     1261 aacatgttta accgtaattg aatcttcatt atagttaatc atcgtatatc tgccatctgt
     1321 taaatgcttc aatatttcta ccgcttcatt aataacttga ggcaaacgtt tatctttaat
     1381 ttgtttaatg tgttcatcaa ctaaactttg taaataactt aaacttgccc aatcttttgc
     1441 gatatcatta agttgatttt taagactgtg atattcatgt cttaaattag caagcgttgt
     1501 atcagtttcc atgtgattga tttgtgcact taaatcactg acttgtgctt gcatttcaag
     1561 atattgctca ttatattcgt caacttgagt agccaataaa tgatcttctt cttcaagttg
     1621 tgcagttgtt ttttcactta aactagaact taattcataa gaatagtttt ggttctcaag
     1681 atatttagtt aaatcattaa aacgactcaa attactagta taagtttggt aatcttcatg
     1741 atgttggtaa aaatcttctt cagtaccaac attgataaaa tcgaatagtg ctgtaatttc
     1801 tttattattt tcttctaatt gagcatttaa atgatttaat tcatttgtaa caagtttggt
     1861 attttcagca ttaatacgcc atttttcatt cgtgtcttca gctgatttca accattgttg
     1921 cacatcgtgg aataaagata atttgttgaa ataaacaaat tgtgattttg taacagcttc
     1981 agcatgattg tagaatgtat ctaattcttg aaccaattgc tggcgttgtt gatttaaatc
     2041 actgatatgt tgatctaatg ctttaatatt cgccattgta gaaatactat caacaattaa
     2101 atcatttgaa attttagatg ataagtataa ttcatcctta acgttctcaa ctgtcgattg
     2161 taattcatca tgacgccctt tcgcatcatt taaacgacct tcaatatact gacgtttctc
     2221 ttctaaaata tctttatttt tcaaagcttg ttgccagtga tcacgaatgc gatattgctc
     2281 atcaagatca aaatctaagt cataattttc atctaaaatg gctagttgtg ctttaatttc
     2341 ttcgatttca tctgtgatgg cctcgctata atctacttct tttgatttag acatgatgat
     2401 accgataaca aatactaaag ttaatactgc gaaaataata ccaaacaaca tgttgtttga
     2461 aataaatgag aaggcagtta aaccaatacc tactaatgtt aaaagaataa acgttgttcg
     2521 taacaatttt tgacgttttt gtttttcttg ttgctcaatt tcgaaacgtt ctttcaattt
     2581 gctatacaag ttctcttttt cgtttaattc aatgacttgt tgtgagtatt cttttttctt
     2641 ttcaaaagtc tcttcaggaa ctattttttc ttcaacagaa tctagttcgc tatgaaccgc
     2701 attatcttcg attttatttt cttctaaact acgttctaat tgtttaatgt atgcagcttg
     2761 ttcttgttta ttcttgattt gctcactgac ataacttttc attgcctctg aactatctac
     2821 gtcatgatgc gtttcagacc aaccaatatt tgcttgcaat tcatctttat cacgttgttt
     2881 attcgcaata tccttttcga ttgcagtaag ttcaaattct ttatttttaa tttcattttc
     2941 ttgttgattc aaactaatga aggcgtcaat atcagattgt ttaactggct ctaattgagt
     3001 cgcttcttct ttaagttgag ctaaacgctc atttcttaaa ccaatatctc tttctaacga
     3061 ttgcttatgc gctcgcgctt tttcgtaacg atccacacct ttttctggga atgtgattgg
     3121 ctcaatattt aactgttgtt ctagagactt ccattcttgt gaatgatcat gtaaagcaac
     3181 ctctttttgt ttttcttcat gcatttttga taattgattt aaattatgct ttaaattctc
     3241 taatcgacgt gatgatttat ctcgatcatc tactaagcga tgatatgttt ctagctttgc
     3301 ttcttcttca cgaatttgac tttctagttg ttttaattgc tcaatttgtt gattaatgat
     3361 cggattttta cctgattttt tatataattc atctttttta cgattaatca cttcgcgcat
     3421 tgacgtgaat tcagttgatc ctaaagcccc tgcttgtaat aaataatctt gcaattgttt
     3481 ttcatttaga tttctatgaa tgtcttgaag ccctagtaca tcaaatgaaa agataccttg
     3541 atatgtcttt ttagaaatat aattaagttt cttttgtaac caagcatcat cacgcacagc
     3601 accattaggt aaatatactt tcacatcacc ttgagcactg cctttaattc gttcaacttc
     3661 aatctctaag ccatcatcaa gaataagtac taatttacca ccgtattggt tacctagacg
     3721 tggttctagt cttggctctt tagacttttt agttggaaaa ccaaataata tcgaatggat
     3781 gaatgcttga atcgtcgatt tacccgcttc attttcacca aaaatttcag tgaagttttt
     3841 attaaattca attttacgtt gaacaaattg accgtaacca taaatttcaa gtgatttaat
     3901 tatcattttg ttcacctctc atttcagctt ttaatatttc ttcagcacga ttaactaatg
     3961 ctgtatggtc gaatgttcca taatcgtcta ggaacttaga tgcccttgga tttaaatata
     4021 aatctgacat cgctttatca aaaacagttt gatcgactaa taattccgct gaaaattcat
     4081 taactaaagg tgactcatca ttttgtgcat attgtatttt taactcatca atatatacaa
     4141 attgattttc gttttcttca taatctgtaa tcatttcttc aacttgtaat aaatcttgag
     4201 gtgaaattaa tgtctcacta ttaataacaa gcgttaaacg ataaaaggct tttccttctt
     4261 ctctcacttg ttctttaaag ttttgaatga cctcgtataa accttgctta gatgtcttat
     4321 ccgtttcaat agttgcttct tcaaatctaa tatactgtgt aggataaaat ttagtcttta
     4381 attttaagtg gtcaccctcg attaataagc aacctttttc accttgctca ttaaaatgtc
     4441 taccttgaat attacctgaa tagttaatta caggcatatc acttaattgt tgacgttcat
     4501 gtatatgacc taaagcccaa taatgataca atttactgtt taaatcttct aaaatgaact
     4561 cggtatatct ttcgttaact gaagatttac tatacgtacc atgcaagaca ccaatatgta
     4621 tgcctttttg gccttgactt gatggatatt catcaatctt gttctcataa cttgctctat
     4681 tttcataact aaatccgtga atataaattg tttcaccaga tttagtaatt gcttcatacg
     4741 tctcaacttt atttgaaaat acagaaacat tatctggcca gtttgatgaa atctttgatg
     4801 ataaaggatc gtgattacca tgacaaacat aaacaaagat ttgttcattt tgtaaacgtt
     4861 caaattgctg ttttaagaaa atttcagcac gtaatgttct gttttcacta tcaaataaat
     4921 cacccgcgat aattacaaaa tcaacatctt gttgtaatgc aatatctaca atatttttaa
     4981 aactttcata agcacttttt tgaacatcct caaaaatttt agggcttata tgactcttag
     5041 atttgaaagg actatctaaa tgtaaatccg aacaatgaat aaatttaacc atacatccat
     5101 atctccttaa ttaaaggttc taacatcaat tttgcaaaat ccaactatac aaagcattct
     5161 aatcactata ttcatactta taggtgtagt gattcattag ctgatttatt aaaaccagtc
     5221 gccattttta tttcgttaaa aaattattat ctacacaata ataaagtgct ttagtcaaat
     5281 cgtgatgttt tatttattaa caagtttatt atttcttcat tttaccataa tacgcttcat
     5341 atcgtcgatg aacatatgaa attgagacac atttataaca gtttaaaatt ttatttgtag
     5401 cgtgcgttga tttttttcaa aaatttcagt cggaaatgca atttccgtaa ttgttaacac
     5461 cgctagtgaa ccaactgaca cattaaagat ttacatgatt actctactag atttgctcgg
     5521 cagtaatgac caattagaaa ctaaatgtca aaactatcat tgcacttaat acgccaaaca
     5581 ttttcaggcc gttcttaatg agtcattaaa agtgaaacgc ttttccatca gtccgataca
     5641 tgaccgaact aatagtatta tgttgaaaac tttacacttc aaaatgcaaa aaggaagtta
     5701 ggacacgttt actagcctaa cttccgttac tgaatgcatc tgcccaattt gacatagaac
     5761 ctctaacttc cgttatcaag ctcatctgcc cgttttggca tagagctagc catgtttgat
     5821 tgcccagctt ccgtcgtcca ttctgagcta aagctaggac atgtttagta gcctaaattc
     5881 cgatagtgaa cgcatctgcc caatttgaca gagagcctct aacttccgtt atcaaaacta
     5941 aaaaaactac ccacaaagtc tcggactctg tcggcagtct attcctctat ttaaaggatt
     6001 gaaaaatatt attttaatat tagtcagcgt aaatttcgtc taatggttta acgataattt
     6061 ggttgatttc ttggaatact tgactcattt tttgttcagc attcattaat gcagagatgt
     6121 tttcatcttt ttcaattgct tgcgcttgtt cttgcgcttt ttgtaaatct tcttcagcaa
     6181 tttcttcacc ttgcatttgt ttttgttgga agttaatttg agtttcacgg aactcatcga
     6241 ataacttttt agattcttcg ttagctttta cattagcgaa tgcttctttg attgctttgt
     6301 attcttcgct ttctcttaaa gcttgttcta attgatttgc ataatcatat aaatttactg
     6361 ccatggttat agcactcctt tgttgtgatg tttattaaaa cgttcgatat tactttacca
     6421 catctcgctt ttttagacaa agattgcaac caaaccttgg aaaaatccaa taataccacc
     6481 taaaatgaaa cctaatgaca taatcaactt tagttctttg ttagcaattt ctataattaa
     6541 tttttcaata taatctaagt caaacgtatt aatttgttct tcaattaatc ctcgtaaatc
     6601 cacttttttc ataattgtag ataagtgaat cgataatttt tcaatgatta aatttgctaa
     6661 tttgctagag agttgacctt ctaaataatc aacgaattga ggcattaacg taaccactgg
     6721 cttattcgct tgattgcttg catatgttgt tacatacact gatagatttt cagcgatttc
     6781 attaaactgc gatgcatcta acagttcatt caatggttta tctttaaaag tttgatattc
     6841 attggtaatt aacgatgtca caattgttct tgctttagga tgagatgtta aacgtataag
     6901 ttcttgttga atgcgatctg caatgctctc ttttgtcata aacatttgca acataccaat
     6961 taacttacct ttctcatgga aaaaagtatc caacatatca ttaatatctt gtgtgccttt
     7021 tgcagatgat aaataattcc ttgcacgatc acataataag tctgttgcgt tatctacatg
     7081 ctgatctaaa aatgttacaa gttgatttgg caataaagat gctattgttt ggttttgatg
     7141 ctttgtataa taattattca attgtgattc aatatattga tttccattag tttgtaatac
     7201 ttgttctaaa tcaatatcga tttgagaagt aatttgtttt attgacaatt gatctttcgt
     7261 caacttttgt aactgctgtt gaatcataga ttctattgct tgctgtgatt gctcgctttt
     7321 taatttttca ttaattaaag tttctgtaag caaatgctct tcaatcactt ggccaatttt
     7381 agttgcaatt tcttcgcgtc ttttcggtat taaacctggt gtaaatggga ctctaaattt
     7441 aaatatatag tatggtttaa aggggtgaaa cagcattcta attgcaatta cattagtaat
     7501 gcccccaatt atcgccccta ctacaatcat aaatatgata ataaatagtg cattcataat
     7561 tttgactcct ttgtgacaac atagttattc attataccac tatttttatt ttttaattca
     7621 ttaatcattt cactttctgc tcaatttatt attagtttac atacaaaaag cgatgcctca
     7681 cctctcgaaa caggtgtgtt acatcgcttt tattatttat taaaagtata taggcctaga
     7741 tatgtactaa ttcttcttta gcatttctgt taaagtatgt ttcagcttct ctcattttag
     7801 aagtaccgaa aattggttgg ttatctggat ttaatacacg ataaatattg cttactttgt
     7861 tactttgtaa aatgtaccca ttacgagtct caatgttatt ccaataaata tcacttgttg
     7921 gtgcatggtt tggatatgca cagtgatttc ttgaaattgt ttgaacgatt tctaatggat
     7981 cacagccaaa tgtactgttt aatacttcag agtctctgaa taatgcacaa attgaaatac
     8041 aagttgtcca gtttggtaat actctctctt tttcgatttg tactaaagtc tttttagaaa
     8101 gtccaattgt ttgcgccata gtatcttgcg tataaccagc ctctatacga accattttaa
     8161 attttgtttg aattaaatct gtaaaactct gtctatccat tctgttatct acctttctgt
     8221 ttggggaatt ttatccggac acaagaaatt gcaataatac acatttcttg aaacacagat
     8281 tacatcttaa tatattttta
//
LOCUS       DAOBBH010000111         8262 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.111, whole genome shotgun sequence.
ACCESSION   DAOBBH010000111 DAOBBH010000000
VERSION     DAOBBH010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8262
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.111"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            59..409
                     /gene="sarV"
                     /locus_tag="P6L01_001787"
     CDS             59..409
                     /gene="sarV"
                     /locus_tag="P6L01_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarV"
                     /protein_id="HDP3030853.1"
                     /translation="MSNKVQRFIEAERELSQLKHWLKTTHKISIEEFVVLFKVYEAEK
                     ISGKELRDTLHFEMLWDTSKIDVIIRKIYKKELISKLRSETDERQVFYFYSTSQKKLL
                     DKITKEIEVLSVTN"
     gene            complement(511..1722)
                     /locus_tag="P6L01_001788"
     CDS             complement(511..1722)
                     /locus_tag="P6L01_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDP3030854.1"
                     /translation="MTNQGSLNKSPIFTKSFTINFFVNFIVYLCMYLLLVVIAGYSKQ
                     AFDASDSLAGLVVGLFIVGSLIGRFATGKFVNQIGPKRLLFIGLIALIITQLLYFIDG
                     SLAFLIFVRLINGIATAVVTTATGTIAAYVTPVNRKSEGISLFSLSLVLGTAIGPFLG
                     MLLITKYAIDLLFIICVILGILGLIISLFIKVDFEVTNNKTETDVINKPRFSIHQFIA
                     KEAIPVAVIMLLIGVTYASILTYLQAFALERHLVTAASYFFICYAIASLITRPIAGRL
                     MDDKNENIIVYPAFIMLFLSFVCLISSYQSWLILLAGACLGLGYGNLSSAMQSIAIKV
                     SPPIKYGIATSTFYVGLDAGVGFGPSFLGLFTHMFSYSEIFGFMAALAIITMLVYFLI
                     HGRHVTRNAMN"
     gene            complement(1715..2155)
                     /locus_tag="P6L01_001789"
     CDS             complement(1715..2155)
                     /locus_tag="P6L01_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HDP3030855.1"
                     /translation="MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKH
                     QPTTLIEISHRRAIEKPTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEI
                     VCLDVQKLQQAVVAKTNISQDQMQETINVMNQIHEILLKEAHND"
     gene            2317..3081
                     /locus_tag="P6L01_001790"
     CDS             2317..3081
                     /locus_tag="P6L01_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDP3030856.1"
                     /translation="MILKFDHIIHYIDQLDRFSFPGDVIKLHSGGYHHKYGTFNKLGY
                     INENYIELLDVENNEKLKKMAKTIEGGVAFATQIVQEKYEQGFKNICLRTNDIEAVKN
                     KLQSEQVEVVGPIQMERDTHKDGKVKWQLLYIMNQDDDEIKPPFFIQWEESDSMRTKK
                     LQKYFQKQFSIETVIVKSKNRSQTVSNWLKWFDMDIVEENDHYTDLILKNDDIYFRIE
                     DGKVSKYHSVIIKDAQATSPYSIFIRGAIYRFEPLV"
     gene            complement(3073..3324)
                     /locus_tag="P6L01_001791"
     CDS             complement(3073..3324)
                     /locus_tag="P6L01_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030857.1"
                     /translation="MRTIILSLFIIMNIVAIIMTLSQPLTVNYFSLRVILIFFTFILS
                     IFFILIKSSRLNNILTILSIVLVIIHMGILAHSTYVYLY"
     gene            3713..4978
                     /gene="femX"
                     /locus_tag="P6L01_001792"
                     /gene_synonym="fmhB"
     CDS             3713..4978
                     /gene="femX"
                     /locus_tag="P6L01_001792"
                     /gene_synonym="fmhB"
                     /EC_number="2.3.2.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase"
                     /protein_id="HDP3030858.1"
                     /translation="MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIA
                     VGRDGEVQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKA
                     YAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELL
                     NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYD
                     ALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMETSEKQAKKAQNMIND
                     AQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNH
                     HMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYIL
                     NQPLYQLIEQVKPRLTKAKIKISRKLKRK"
     gene            5095..8262
                     /locus_tag="P6L01_001793"
     CDS             5095..8262
                     /locus_tag="P6L01_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDP3030859.1"
                     /translation="MIKKLLQFSLGNKFAIFLMVVLVVLGGVYASAKLKLELLPNVQN
                     PVISVTTTMPGATPQSTQDEISSKIDNQVRSLAYVKNVKTQSIQNASIVTVEYENNTD
                     MDKAEEQLKKEIDKIKFKDEVGQPELRRNSMDAFPVLAYSFSNKENDLKKVTKVLNEQ
                     LIPKLQTVDGVQNAQLNGQTNREITLKFKQNELEKYGLTADDVENYLKTATRTTPLGL
                     FQFGDKDKSIVVDGQYQSVDAFKNINIPLTLAGGQGQSQSQSDNKQNSAMSDVNQASP
                     QQNSKASAPNNISGMPTAKLGDLADITVGDVRTSISKTNGKDAVNLQITKAQDANTVQ
                     VAKDVQRKIDTFVDENKDFNVTKTMDTAKPVEKSLYTMVEKASLGTIVAIIVILLFLR
                     NIRTTAISIISIPLSLLMALIALKLSDVSLNILTLGALTVAIGRVIDDSIVVVENIYR
                     RLTDSEEQLKGENLIISATTEVFKPIMSSTLVTIIVFLPLVFVSGSVGEMFRPFALAI
                     AFSLLASLLVSITLVPALAATLFKKGVKRRNKQHQEGLGVVSTTYKKVLHWSLNHKWI
                     VIILSTLILVATIVFGGPRLGTSFISAGDDKFLAITYTPKPGETEQAVLNHAKDVEKY
                     LKQKKHVKTIQYSVGGSSPVDPTGSTNSMAIMVEYDNDTPNFDVEADKVIKHVDGFKH
                     PGEWKNQDLGTGAGNKSVEVTVKGPSMDAIKSTVKDIEQKMKQVKGLANVKSDLSQTY
                     DQYEIKVDQNKAAENGISASQLAMHLNENLPEKTVTTVKENGKTVDVKVKQNKQTDWS
                     EDKLNNITLKKPTGGTIKLGDIATLVKTTTPSKLTQEQGDYATTVSAKVTNKDVGGTT
                     RQVMSKINNLDKPNNVKVNIGGASDDINNAMTQLAFAMLAAIIIVYLILVITFKGGLA
                     PFTILFSLPFTVIGVIIALLITGETISVPSLIGMLMLIGIVVTNAIVLIDRVINNEQQ
                     GMEMKEALIEAGGTRIRPILMTAIATIGALVPLLFGQDSSILISKGLAATVIGGLISS
                     TLLTLVVVPVIYEILFTLKKRFTKR"
BASE COUNT         3106 a         1144 c         1542 g         2470 t
ORIGIN      
        1 aattgtataa taatatgaat tacaaacatc taagaagaaa cataggaggc atcatattat
       61 gagtaataaa gttcaacgtt ttatagaagc agaaagggag ttaagtcagt taaagcactg
      121 gttaaaaaca acacataaga tttcaattga agaatttgta gtacttttta aagtgtatga
      181 agctgaaaag attagcggta aagaattgag ggatacatta cattttgaaa tgctatggga
      241 tacaagtaaa atcgatgtga ttatccgtaa aatctataaa aaagagctta tttctaaatt
      301 gcgttctgaa acggatgaaa gacaagtatt ctatttctat agtacttctc aaaagaaatt
      361 gttagataaa attactaaag aaatagaagt gttaagcgtt acaaactaaa aacttaaaaa
      421 gcatgccaat cagtacatca tatttgcgtc ttggggacag acaaatgatg aatagagatt
      481 ggcatgcttt ttatttttga atataaatat ttagttcatg gcatttctag ttacatgacg
      541 tccatgaatt aagaagtaaa caagcatagt aatgattgct aaagcggcca taaagccgaa
      601 gatttcacta tatgaaaaca tatgagtaaa taacccaagg aatgatggac cgaagccgac
      661 acctgcatct agaccaacgt aaaaagtaga tgtcgcgata ccatatttaa tcgggggtga
      721 gacttttatc gcaatagatt gcattgcaga tgataaattt ccatacccta aacctaggca
      781 agcaccagca agtaatatta accagctttg atagcttgaa attaagcaca caaatgaaag
      841 gaaaagcatg ataaatgctg gatagacaat aatattttca tttttatcat ccatcaatct
      901 accagcaata ggtctagtaa ttaacgatgc tatagcatag caaataaaga aatagcttgc
      961 tgcagtgact aggtgtcgct ctaaagcaaa tgcttgtaaa taagttagga ttgacgcata
     1021 ggtaacgcca attaaaagca taattacagc aacaggaatg gcctcttttg caataaattg
     1081 atgaatacta aatcttggtt tattaatgac atcagtttca gttttattat ttgttacttc
     1141 gaaatcaact tttataaata atgagataat gagtccgagt atgcctaata tgacacaaat
     1201 aataaacagt aaatcaattg cgtattttgt aataagtaac atgcctagaa atgggccaat
     1261 cgctgtacct aatactaaac ttaaggaaaa taaactgatg ccttcacttt ttctattaac
     1321 aggggtaacg tatgccgcaa tagtacctgt tgcagttgtc acaactgcag ttgcgatacc
     1381 gtttatgaga cgtacaaaga ttaaaaaagc taaagatcca tcaataaaat aaagtaattg
     1441 cgtgataatt aaagcaatta aaccaataaa taataatcgt ttaggtccga tttgatttac
     1501 aaatttacct gtagcaaatc gaccaattaa agacccgaca ataaatagtc ctacaactaa
     1561 tccggctaat gaatcggaag cgtcgaaagc ttgcttacta tagccagcaa taactacaag
     1621 taacaaatac atgcataggt atacgataaa gttgacaaaa aagttgattg taaaactttt
     1681 ggtgaaaata ggtgatttat ttaatgagcc ttgattagtc attgtgtgcc tcctttaata
     1741 atatttcatg aatttgattc atcacattta tggtttcttg catttgatct tgcgaaatgt
     1801 ttgttttggc aactactgct tgttggagct tttgtacatc aagacaaaca atctcatata
     1861 attcatgccc tttaggtgtt aaagttaaaa acttttgtcg tttatcctct aaactgtttt
     1921 ctactgttat aaggtcattt tctattagag cttttaaagt ttttcttgca gtaggctttt
     1981 caattgcacg tctatgtgaa atttcaatga gagtagtggg ctgatgttta gcgatatcgc
     2041 gtaagattaa ccattgacca taatatatat tgtgtttttc caaaatcggc tctgctaatt
     2101 ttaaataggg gcgatatatt gttataaaac tattgaaaaa ttcttgtgat agcatagtga
     2161 catctcctaa gacaaaatag ttagcttagc taaccttttt acaacaatag taattataaa
     2221 acgggagcaa ttagaaatca atatataatt attaagagca aaaataatta tactttgtta
     2281 aaataagcgt aattacatgt aaataggggg atactaatga tattgaaatt tgatcacatc
     2341 attcattata tagatcagtt agatcggttt agttttccag gagatgttat aaaattacat
     2401 tcaggtgggt atcatcataa atatggaaca ttcaataaat taggttatat caatgaaaat
     2461 tatattgagc tactggatgt agaaaataat gaaaagttga aaaagatggc aaaaacgata
     2521 gaaggtggag tcgcttttgc tactcaaatt gttcaagaga agtatgagca aggctttaaa
     2581 aatatttgtt tgcgtacaaa tgatatagag gcagttaaaa ataaactaca aagtgagcag
     2641 gttgaagtag tagggccgat tcaaatggaa agagatacac ataaagatgg taaggtaaag
     2701 tggcaattgc tttatataat gaatcaggat gatgatgaaa ttaagccacc attttttatt
     2761 caatgggaag aaagtgattc catgcgtact aaaaaattgc aaaaatattt tcaaaaacaa
     2821 ttttcaattg aaactgttat tgtgaaaagt aaaaaccgat cacaaacagt atcgaattgg
     2881 ttgaaatggt ttgatatgga cattgtagaa gagaatgacc attacacaga tttgatttta
     2941 aaaaatgatg atatttattt tagaattgaa gatggtaaag tttcaaaata tcattcggtt
     3001 atcataaaag acgcacaagc aacttcacca tattcaattt ttatcagagg tgctatttat
     3061 cgctttgaac cattagtata aatatacgta agtgctatga gcgagaatgc ccatatgaat
     3121 aatgacaagc acaatggaaa gaatcgttaa tatattattt aatcgggatg acttaattaa
     3181 aatgaaaaag attgataata taaatgtgaa aaagataagt ataacccgta aactaaagta
     3241 attcacggtg agaggttgac tcaatgtcat aatgattgca acgatgttca taattataaa
     3301 tagacttaaa ataattgttc tcatatcaaa cacctcattg ttagattatt gacattataa
     3361 caggggtaat tgtatatgaa cattaatgtg gttgcttgag gaaaaattta ttcattgaag
     3421 tcaagttggt tcattttaga aatgaatatc gtgttagatg atgaaagtat attgaagtat
     3481 aggtaactag ttgaaaagta ttaattgtac gataacatta aatttaacac gaaacataga
     3541 tataaaatga ttcacaatta aaatgggtaa atttgaactt gctaaactat taattggagc
     3601 atggacattt caaaaataag agttcaaatc ttacacaagc tctgaatcga cactataaga
     3661 tacaaactgt ataattaaag gtattgttaa atagaaggag atatcataaa tcatggaaaa
     3721 gatgcatatc actaatcagg aacatgacgc atttgttaaa tctcacccaa atggagattt
     3781 attacaatta acgaaatggg cagaaacaaa gaaattaact ggatggtacg cgcgaagaat
     3841 cgctgtaggt cgtgacggtg aagttcaggg tgttgcgcaa ttacttttta aaaaagtacc
     3901 taaattaccg tatacgctat gttatatttc acgtggtttt gttgttgatt atagtaataa
     3961 agaagcgtta aatgcattgt tagacagtgc aaaagaaatt gctaaagctg agaaagcgta
     4021 tgcaattaaa atcgatcctg atgttgaagt tgataaaggt acagatgctt tgcaaaattt
     4081 gaaagcgctt ggttttaaac ataaaggatt taaagaaggt ttatcaaaag actacatcca
     4141 accacgtatg actatgatta caccaattga taaaaatgat gatgagttat taaatagttt
     4201 tgaacgccga aatcgttcaa aagtgcgctt ggctttaaag cgaggtacga cagtagaacg
     4261 atctgataga gaaggtttaa aaacatttgc tgaattaatg aaaatcactg gggaacgcga
     4321 tggcttctta acgcgtgata ttagttactt tgaaaatatt tatgatgcgt tgcatgaaga
     4381 tggagatgct gaactatttt tagtaaagtt ggacccaaaa gaaaatatag cgaaagtaaa
     4441 tcaagaattg aatgaacttc atgccgaaat agctaaatgg cagcagaaga tggaaacatc
     4501 tgaaaagcaa gctaaaaaag cgcaaaatat gattaatgat gcgcaaaata aaattgctaa
     4561 aaatgaagat ttaaaacgag acctagaagc tttagaaaag gaacatcctg aaggtattta
     4621 tctttctggt gcactattaa tgtttgctgg ctcaaaatca tattacttat atggtgcgtc
     4681 ttctaatgaa tttagagatt ttttaccaaa tcaccatatg cagtatacga tgatgaagta
     4741 tgcacgtgaa catggtgcaa caacttacga tttcggtggt acagataatg atccagataa
     4801 agactctgaa cattatggct tatgggcatt taaaaaagtg tggggaacat acttaagtga
     4861 aaagattggt gaatttgatt atatattgaa tcagccattg taccaattaa ttgagcaagt
     4921 taaaccgcgt ttaacaaaag ctaaaattaa aatatctcgt aaattaaaac gaaaatagat
     4981 taacgactga aatctgaacg ctcataagac tgtcatttgc gttcagattt ttttacacaa
     5041 tatagaatgg ttgagtaaaa tatttttgaa tatagtgaaa gagggggaag tactgtgata
     5101 aaaaagctat tacaattttc tttggggaat aagtttgcta tctttttaat ggttgtttta
     5161 gttgtcttgg gcggtgtata cgcgagtgct aaattgaaat tagaattact accaaatgta
     5221 caaaatccag ttatttcagt tacaacaaca atgccgggtg caacgccaca aagtacccaa
     5281 gatgaaataa gtagtaaaat tgacaatcaa gtaagatcgt tggcatatgt gaaaaatgtt
     5341 aaaacgcaat ccatacaaaa tgcttcaatt gtaacagttg aatatgaaaa taatacagat
     5401 atggataaag cagaagaaca gcttaaaaaa gaaatcgata aaattaaatt taaagatgaa
     5461 gttggtcaac cagaattaag acgtaattcg atggacgctt ttccggtttt agcatattca
     5521 ttttcaaata aagagaatga cttgaaaaaa gtaacgaaag tactgaatga acaattaata
     5581 ccaaaattac aaacggtaga tggtgtgcaa aatgcgcaat taaatgggca gacgaaccgt
     5641 gaaatcaccc ttaaatttaa gcaaaatgaa cttgaaaaat atgggttgac tgctgatgat
     5701 gtagaaaact atctaaaaac agcaacaaga acaacaccac ttggattgtt ccaatttggt
     5761 gataaagata aatcaattgt tgttgatggt caatatcaat ctgttgatgc ttttaaaaac
     5821 ataaatattc cattaacgtt ggcaggaggg caagggcaat ctcaatccca aagtgataat
     5881 aaacaaaatt cagccatgtc agacgttaat caggcatcac cgcagcaaaa ttcaaaagcg
     5941 tcagcaccaa ataatataag tggtatgccg acagcgaaac taggagattt agctgatatt
     6001 acagttggtg atgtgcgtac ttctatttct aaaacgaatg gaaaggatgc ggttaatcta
     6061 caaataacta aagctcaaga tgccaataca gttcaagtag ccaaagatgt acaacgtaaa
     6121 attgatacat tcgttgatga aaataaagat tttaatgtca caaaaacaat ggatactgca
     6181 aagcctgttg agaaatcact ttatacgatg gttgaaaaag catcattagg tacaatcgtg
     6241 gcaattatag ttattttgct gtttttaaga aacattcgta cgacggcaat ttctattata
     6301 tccattccgt tatcacttct tatggcgctt attgctctga aattgagtga tgtttcattg
     6361 aatatactaa cgttaggtgc attaacagta gcgattggac gtgtgataga cgattcgatt
     6421 gtagttgttg aaaatattta tcgacgctta acagattcag aagaacaact aaaaggtgaa
     6481 aatttaatta tcagtgcgac aactgaagta tttaaaccaa taatgtcatc gacactagtt
     6541 actattatcg tcttcttacc acttgtgttt gtatcaggtt cagtaggcga aatgtttaga
     6601 ccttttgcat tggctattgc atttagttta ttagcatcgt tattagtgtc aattacactc
     6661 gttccagctt tggcagcaac actatttaaa aaaggcgtta aacgtcgtaa taaacaacat
     6721 caagaaggat taggtgttgt tagtacaact tataaaaaag tattgcattg gtcactaaat
     6781 cataagtgga ttgtaattat attaagtaca ttaattttgg ttgcaactat tgtatttgga
     6841 ggaccgagac taggcactag ctttatttca gcaggtgacg ataaattttt agctattact
     6901 tatacaccga agcctggtga aacggagcaa gcagtgttga atcatgcgaa agatgttgaa
     6961 aaatatttaa aacagaaaaa gcatgtaaaa acaattcaat actcagttgg tggtagtagt
     7021 ccagtagatc caacgggtag tacaaatagt atggcaatca tggttgaata tgataatgac
     7081 acgcctaatt ttgatgtaga agcggataag gttattaaac atgtagatgg ctttaaacat
     7141 cctggagagt ggaaaaatca agatttagga acaggtgcag gtaataaatc tgtagaggtt
     7201 actgtgaaag gcccatcaat ggatgccata aaatcaactg taaaagatat tgaacagaaa
     7261 atgaaacagg ttaaaggact agccaatgtc aaatctgatt tatcgcaaac atatgatcag
     7321 tatgaaatta aagttgatca aaataaagcg gcagaaaatg gtatttctgc aagtcaactt
     7381 gcaatgcact tgaatgaaaa cttaccagaa aaaacagtta cgactgttaa agaaaatggt
     7441 aaaactgttg atgttaaagt caaacaaaat aagcaaacag actggtcaga agataagttg
     7501 aataatataa ctttgaaaaa gccgactggt ggtacgatta aattgggaga tatcgctacg
     7561 ttagttaaaa caacgacacc aagtaaattg acgcaagaac aaggtgatta tgcaacgacg
     7621 gtatctgcta aagtaacaaa taaagatgtg ggtggcacaa cacgacaagt gatgtctaaa
     7681 ataaataatt tggacaaacc gaataatgta aaggttaata tcggtggtgc atcagatgat
     7741 attaacaatg caatgacaca attagccttt gcaatgttag ctgcaatcat tatcgtatat
     7801 ttaatcctag ttattacatt taaaggtggc ttagcgccat ttacaatttt attctcttta
     7861 ccatttacag ttatcggtgt aattattgca ctattaatca caggagaaac aatatcagta
     7921 ccaagtttaa ttggtatgct aatgttaatt ggaatcgtag taacaaatgc cattgtgtta
     7981 atagaccgtg tcattaataa tgagcagcag ggcatggaga tgaaagaagc attaatcgaa
     8041 gcaggcggta ctagaattag accgatatta atgacggcga ttgcaacaat cggtgcatta
     8101 gttcctttgt tatttggtca agatagctcg attcttattt cgaaaggatt agctgctaca
     8161 gttattggtg gtttaatttc atcaacttta ttaacacttg tagttgtacc agtaatatat
     8221 gaaattctct ttactttaaa aaaacgattc actaaacgat ag
//
LOCUS       DAOBBH010000112         8045 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.112, whole genome shotgun sequence.
ACCESSION   DAOBBH010000112 DAOBBH010000000
VERSION     DAOBBH010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8045
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.112"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            155..871
                     /locus_tag="P6L01_001794"
     CDS             155..871
                     /locus_tag="P6L01_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDP3030860.1"
                     /translation="MGRKWNNIKEKKAQKDKNTSRIYAKFGKEIYVAAKSGEPNPESN
                     QALRLVLERAKTYSVPNHIIEKAIDKAKGAGDENFDHLRYEGFGPSGSMLIVDALTNN
                     VNRTASDVRAAFGKNGGNMGVSGSVAYMFDHVATFGIEGKSVDEILETLMEQDVDVND
                     VIDDNGLTIVYAEPDQFAVVQDALRAAGVEEFKVAEFEMLPQTDIELSEADQVTFEKL
                     IDALEDLEDVQNVFHNVDLK"
     gene            871..1344
                     /locus_tag="P6L01_001795"
     CDS             871..1344
                     /locus_tag="P6L01_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDP3030861.1"
                     /translation="MKSAEQWIDELQLESHPEGGFYRETIREVLKDGRRAPFSSIYFL
                     LTDDNISHFHRIDADEVWYYHAGDSLTIHMINPDGEYTTATLGTDIQNGDVLQYVVPK
                     GTIFASSIEISNTYSLVGCMCQPAFEFKQFELFKQSELITQYPHLKSVIEKYALK"
     gene            1436..1552
                     /locus_tag="P6L01_001796"
     CDS             1436..1552
                     /locus_tag="P6L01_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030862.1"
                     /translation="MLQLFLMKIALNDFENDKSVLFIFLKNHFYEEMCVLSN"
     gene            1697..2341
                     /locus_tag="P6L01_001797"
     CDS             1697..2341
                     /locus_tag="P6L01_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDP3030863.1"
                     /translation="MKVKYIDKRHWRRLIDREYTEVKVNNNRFKGIIGLVTMKKVRDP
                     LEVTVVGQNIIVADDNYKWLQILPEKKRYSITVMFDNKGNPLEYYFDINIKNITQKGN
                     ARTVDLCLDVLALPSGEYELVDEDDLMFALESEQITKKQFHEAYMIAHQIMAELENDF
                     KGFQKKIMYCFNKINAKAQKNHQKPQNKTNIEKSKQIKPKQYNQTKNHQQQKKN"
     gene            2457..3323
                     /locus_tag="P6L01_001798"
     CDS             2457..3323
                     /locus_tag="P6L01_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDP3030864.1"
                     /translation="MKIEDYRLLITLDETKTLRKAAEILYISQPAVTQRLKAIENAFG
                     VDIFIRTKKQLITTTEGTMIIEHARDMLKRERLFFDKMQAHIGEVNGTISIGCSSLIG
                     QTLLPEVLSLYNAQFPNVEIQVQVGSTEQIKANHRDYHVMITRGNKVMNLANTHLFND
                     DHYFIFPKNRRDDVTKLPFIEFQADPIYINQIKQWYNDNLEQDYHATITVDQVATCKE
                     MLISGVGVTILPEIMMKNISKEQFEFEKVEIDNEPLIRSTFMSYDPSMLQLPQVDSFV
                     NLMASFVEQPKA"
     gene            3455..4675
                     /locus_tag="P6L01_001799"
     CDS             3455..4675
                     /locus_tag="P6L01_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HDP3030865.1"
                     /translation="MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGM
                     TTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTI
                     WLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPF
                     IGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKD
                     KTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILG
                     VLSSRLHTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQ
                     DILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFF
                     TKNQKITKEDMIST"
     gene            4672..5160
                     /locus_tag="P6L01_001800"
     CDS             4672..5160
                     /locus_tag="P6L01_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HDP3030866.1"
                     /translation="MTIILWLLIIAAFMLAFVGLIKPIIPSVLVLWVGFLIYQFGFHN
                     QHLSWVFYVSMALLTILILCADFLANKYFVNRFGGSKFGEYAALIGVVIGCFVLPPFG
                     IIIIPFILVFIVELIQGYSFERAVKVSIGSIVAFLTSSIAQAIIMFIMIVWFFIDALL
                     IN"
     gene            complement(5259..5948)
                     /locus_tag="P6L01_001801"
     CDS             complement(5259..5948)
                     /locus_tag="P6L01_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HDP3030867.1"
                     /translation="MKSTAQLTKENNVKSLRLSNTDREIFENYMTYMRSDFRVNPHDT
                     ELIINRILKQLLSAEQHGLLALDFFNHDPKAHAIKELKAMPNETFKNIFKYIYQHIVL
                     LIGIVSFLKGFLGFFMEKNGSNLYFVSFPFSVVVGFFIVFLFIWFSFKTIQLQCFNNS
                     NWIWIFTYLAIILLIVGFFYVFFIPQSLLAFGPYIQVSNWVFIIFSFIVMPIGLRIER
                     NISKKHSHTFL"
     gene            6075..6518
                     /locus_tag="P6L01_001802"
     CDS             6075..6518
                     /locus_tag="P6L01_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDP3030868.1"
                     /translation="MRTLNKDEHNYIKQIANIHETLLSQAESNYKCTKLSIALRYEMI
                     CSRLEHTNDKIYIYENEGQLIAFIWGHFSNEKSMVNIELLYVEPQFRKLGIATQLKIA
                     LEKWAKTMNAKRISSTIHKNNLPMISLNKDLGYQVSHVKMYKDID"
     gene            6585..6980
                     /locus_tag="P6L01_001803"
     CDS             6585..6980
                     /locus_tag="P6L01_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030869.1"
                     /translation="MKKLIISIMAVMLFLTGCGKSQEKATLEKDIDNLQKENKELKDK
                     KEKLQQEKEKLADKQKDLEKEVKDLKPSKEDNKDDKKDEDKNKDKDKDKEASQDKQSK
                     DQTKSSDKDNHKKPTSTDKDQKANDKHQS"
     gene            7118..7417
                     /locus_tag="P6L01_001804"
     CDS             7118..7417
                     /locus_tag="P6L01_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030870.1"
                     /translation="MHEQDFRILEGQDITLPELGRELENITGHTIADSTGEIKRVIAH
                     LPNFESDTDTFVATYRLNHQQDFIDATFTALKSDRARLKEVPVHVELISYISKSK"
     gene            7497..8039
                     /locus_tag="P6L01_001805"
     CDS             7497..8039
                     /locus_tag="P6L01_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDP3030871.1"
                     /translation="MIYCETERLILRDWHEDDLLPFQKMNANYDVRKYFPSLLSYRRS
                     ELDMRTMDAVIKDYGIGLFAVEDKESHQWIGFIGLNYIPETSDYPFKELPLYEIGWRL
                     LPEFWGKGLATEGAKATLKLAEEHQIYDVYSFTAEANKASQRVMEKIGMTVYDHFELP
                     NLSKYHLLKRQVRYYINLRK"
BASE COUNT         2935 a         1039 c         1447 g         2624 t
ORIGIN      
        1 tcggcacatt tttaatttat acatatttta aaactaagta acagtttgaa ggaatcgtag
       61 ttcaataatg ttaattgtga aaatgtatat aaacataaaa aaatcatgta taatatatgt
      121 tgttaattaa acagttcgaa agcgagatga cattatggga cgtaaatgga ataacattaa
      181 agaaaaaaag gcccaaaaag ataaaaacac aagtagaata tatgcgaaat ttggtaagga
      241 gatttatgtt gcagcaaaat ctggtgaacc caatccagaa tctaaccaag ctttaaggtt
      301 ggtgcttgaa cgcgctaaga catattcagt gccgaatcat attattgaaa aagcaataga
      361 taaagctaag ggtgctggag acgaaaactt tgatcaccta agatatgaag gatttggccc
      421 aagcggatca atgctaattg ttgatgcgtt aacaaataat gtaaatcgta ctgcctctga
      481 tgtgcgagct gcttttggta aaaacggcgg taatatgggt gtatctggat cagttgctta
      541 tatgtttgat catgtggcaa catttggtat tgaaggaaag tctgttgacg aaatacttga
      601 aacattaatg gaacaagatg tagatgtaaa tgatgtgatt gacgataatg gattgacaat
      661 agtctatgct gaaccagatc aatttgcagt cgttcaagat gcgcttcgtg cagcaggtgt
      721 tgaagaattt aaagttgctg aatttgaaat gttacctcaa acagatattg aactttctga
      781 agcggaccaa gtaacatttg aaaaattaat cgatgcatta gaagatttag aagatgtaca
      841 aaacgtattc cataatgtgg atttgaaata atgaaatcag cagaacaatg gattgatgaa
      901 ttgcaacttg aatcacatcc tgaaggtggt ttctatagag agacaattcg agaagtattg
      961 aaagatggac gcagagcgcc gtttagtagt atttattttt tacttacaga tgacaatatt
     1021 tcgcattttc atcgaattga tgctgatgaa gtatggtact atcatgctgg tgattctcta
     1081 acaattcata tgataaatcc ggatggggaa tatacgactg caacattggg tactgatatc
     1141 caaaatggag atgtattgca atatgtagtg cctaaaggaa caatttttgc ttcttcaatc
     1201 gaaatttcaa atacttatag tttagtaggt tgtatgtgtc aaccggcatt tgagtttaag
     1261 cagtttgaat tgtttaagca atctgaatta attacacaat atccgcatct taaatcagtg
     1321 attgaaaaat atgctttaaa ataaaaatga tcaatgaagt ggtttgaagg ttgttaataa
     1381 acctttgagt cacttcattt ttatatgtat tcttgattga atcagaatag atttgatgct
     1441 tcagctgttt ttaatgaaaa tagcattaaa tgattttgaa aacgataaga gtgtgttatt
     1501 tatatttttg aaaaatcact tttatgaaga aatgtgtgta ctatctaatt aatattgtct
     1561 attttaagta aattgaagcg agttgtagta aaataataat aaatattttc atatgattaa
     1621 caaaaactat aaaactgtat catgacatga catcatttat gtagttttat ataataatga
     1681 caaggagttg gtgactgtga aagtaaagta tatagataaa cgtcactggc gtcgcctaat
     1741 tgatagggaa tacacagagg taaaagttaa taataatagg tttaagggta ttataggctt
     1801 agtcacgatg aaaaaggttc gtgatccttt agaggtgacg gtagttggac aaaatatcat
     1861 tgtcgcagat gacaattata aatggttgca aatactacct gaaaagaaac gttatagtat
     1921 aactgtaatg tttgataata aaggcaatcc attagaatat tattttgata taaatatcaa
     1981 aaatataacg caaaaaggta atgcgcgtac agtagattta tgtttagatg ttttagcatt
     2041 accaagtggt gaatatgagt tggtagatga agatgattta atgtttgcat tagaaagtga
     2101 gcaaattaca aaaaagcaat ttcatgaagc atatatgatt gcacatcaaa ttatggcaga
     2161 gttagaaaat gattttaaag gattccaaaa gaaaatcatg tactgcttta ataaaattaa
     2221 tgcaaaggct caaaaaaatc atcaaaagcc acaaaataaa actaatattg aaaaaagcaa
     2281 acaaataaaa cctaagcaat ataatcaaac taaaaatcac caacaacaaa agaaaaacta
     2341 agtaattcaa gctgcagcca tgccaataaa attggtgaaa ttattcgtta gtaatgaact
     2401 catgaatgct tgtagccatg aaagttcaat aattgaataa tttatggggg gaaattatga
     2461 agattgaaga ctatcgttta ctaataacat tagacgaaac gaaaacgtta cgtaaagcgg
     2521 ctgaaatttt atatatatct caacctgctg ttacacaaag actaaaagct attgaaaatg
     2581 cttttggagt agatattttt atcagaacaa aaaaacaatt gattacaaca actgaaggaa
     2641 caatgattat tgagcatgcc cgtgacatgt tgaaaagaga gcgattattt tttgacaaaa
     2701 tgcaggcaca tattggtgaa gtgaatggaa caatatcaat cgggtgttct tctttgattg
     2761 gacaaacctt acttcctgaa gttttgagcc tatataatgc ccaatttcct aatgttgaaa
     2821 tacaagtgca agttggttca actgaacaaa ttaaagcaaa tcatagagat tatcatgtta
     2881 tgataactcg tggaaataag gtaatgaatt tagctaacac acatttattt aatgatgatc
     2941 attattttat ttttccaaaa aatagacgag atgatgttac aaagttacca tttatagagt
     3001 ttcaagctga tccgatttat ataaatcaaa taaaacaatg gtataacgat aatttagaac
     3061 aagattacca tgcaactatt acagtggatc aagtagcaac ttgcaaagaa atgttgatta
     3121 gtggtgtagg tgttacaatt ttgccggaaa ttatgatgaa aaatatcagc aaagaacaat
     3181 ttgagtttga aaaagtagaa attgataatg aaccgctgat tcgttcgaca tttatgagtt
     3241 atgatccgag catgttgcaa ttgccacaag ttgattcttt tgtaaatctc atggcgagct
     3301 ttgttgaaca accaaaggcg tagttttaga ctaatttaag gtttgtattt aattttaaac
     3361 tattcggtta aattgaacgt agttgtttgc taacgcacca acagcaaaag agcccctaat
     3421 taataaatta aaaggggctc taagaagcgg ggttatggat atgtttgcag cgttattaca
     3481 aataaagaat tataaactct ttgttgctaa tatgtttcta ctaggtatgg gtattgcggt
     3541 tacggtccca tatcttgttc tttttgcaac taaagattta ggtatgacaa caaatcagta
     3601 tggattactt ctagcatctg cagcgattag ccagtttaca gtaaattcaa ttattgctag
     3661 attttcggat acgcatcact ttaatagaaa aattattatt attctcgcat tattaatggg
     3721 tgcgcttggt ttttcaatat acttttttgt agatacaatc tggttattca tattactata
     3781 tgcgattttc caaggattat ttgcaccagc aatgccccaa ctttacgcat ctgctagaga
     3841 atctatcaat gtttcaagct ctaaagatag agctcaattt gccaacacag tattacgttc
     3901 aatgttctca ttgggctttt tatttggtcc atttattggt gcccaattaa tcggtttaaa
     3961 aggctatgct ggattgtttg gtggaacaat aagtatcatt ttatttactt tagtacttca
     4021 agtgtttttc tataaggatt taaacattaa acaccctatt agtacgcaac aacatgttga
     4081 aaaaattgct cctaatatgt ttaaagacaa aacgctttta ttaccattta ttgcatttat
     4141 tttattacac attggacaat ggatgtatac gatgaatatg cctttatttg ttactgatta
     4201 tttaaaagaa aatgagcaac atgtcggtta tttagctagt ttatgtgctg gtttagaagt
     4261 gccatttatg atcattcttg gcgttttatc atctagatta catactcgaa cattgttgat
     4321 ttatggagcg atttttggtg gtttattcta cttcagcatt ggggtattta aaaacttcta
     4381 tatgatgtta gcaggacagg tatttttagc tattttctta gcggttcttt taggaattgg
     4441 tattagttat ttccaagata tcttaccaga ttttccagga tacgcctcaa cactattttc
     4501 taatgcaatg gttattggac agttaggcgg taacctatta ggtggtgcta tgagtcactg
     4561 ggtaggtttg gaaaatgtat tttttgtatc agcagcatca atcatgttag gtatgatact
     4621 tatattcttt actaaaaatc aaaaaattac aaaagaggat atgatatcaa catgacaatt
     4681 attttatggc tacttatcat cgctgccttc atgttagcat ttgttgggtt gattaagccg
     4741 attattcctt ctgttttagt attatgggtt ggctttctaa tctatcaatt tggctttcat
     4801 aatcagcatt tatcatgggt gttttatgta tctatggcat tgctaacaat actaatttta
     4861 tgtgcggact ttttagctaa taaatatttt gtgaatcgct tcggtggttc taagtttgga
     4921 gagtatgcag ctttaattgg tgtggttatt ggatgttttg ttttaccgcc atttggaatt
     4981 attattatac cttttatttt ggtattcata gttgaattaa tacaaggcta ttcatttgaa
     5041 agagcagtta aagtaagtat aggttcaatc gtagcatttt taacaagtag tatagctcaa
     5101 gcaatcatta tgtttataat gattgtatgg ttctttatag atgctttatt gattaattaa
     5161 taaaaagctt attgcaaaat atgtttttcg gtaactgtaa tttagtgatt ttatcattaa
     5221 cagtaccaaa ttcgcatatg atgtaataag ctttttgttt ataaaaatgt atgagaatgt
     5281 tttttcgaaa tatttctttc aatgcgtaat ccaataggca taactataaa tgaaaatata
     5341 ataaataccc aattgcttac ctgaatatat ggtccaaagg caagtaatga ttgtggaata
     5401 aagaatacat agaaaaatcc tacaattagt agaatgattg ctaaataggt gaatatccat
     5461 atccaatttg aattgttaaa acactgcagt tgtattgttt taaaactaaa ccatataaat
     5521 aaaaagacaa taaagaatcc aacgactact gaaaacggga atgaaacaaa atataaatta
     5581 cttccatttt tttccatgaa aaatcctaaa aatcctttga gaaaactaac aatcccaatt
     5641 aatagaacga tgtgttgata gatatattta aaaatatttt taaatgtttc attaggcatc
     5701 gcttttagtt cttttattgc atgtgctttt gggtcgtgat tgaaaaaatc taaggctaat
     5761 aaaccatgtt gttctgcgct taataattgt ttgagtatac ggttaataat taactctgta
     5821 tcatgagggt tgacgcgaaa gtcagagcgc atataagtca tataattctc gaagatttct
     5881 ctatcagtat tgcttaatct taatgattta acattatttt cttttgttaa ttgcgcagta
     5941 cttttcattg ttacttaagc gctcctttaa aaatgtttaa ttcctaatta aaatggaaat
     6001 gattttatag tattaataaa gtcaatcata tcatattaaa cgcataaata taacgattaa
     6061 tattggagag gaaaatgagg acacttaata aagatgaaca taattatatc aagcaaatag
     6121 ctaatataca cgagacatta ttgtcgcaag cagaatccaa ctataaatgt actaaactga
     6181 gtattgctct taggtacgag atgatatgtt caagattaga acatacaaat gataaaattt
     6241 atatatatga aaatgaaggt caattaatag cgtttatttg gggacatttt agtaatgaaa
     6301 aaagtatggt taacattgaa ctgctatatg ttgaaccaca atttcgcaaa ctgggaatag
     6361 ctacgcaact gaagattgcg cttgaaaaat gggcaaaaac tatgaatgca aagcgaataa
     6421 gcagtacaat tcataaaaat aatttgccaa tgatatcttt gaataaagat ttaggttatc
     6481 aagtgagtca tgtgaaaatg tataaagata ttgattagaa ttaggattat gttgctaatt
     6541 catgttaaaa ttaaaaaaga tttaatgacg ttaaggagtt ttatatgaag aaattaatca
     6601 tcagtattat ggcggtcatg ctatttttaa caggttgtgg taaaagtcaa gagaaagcca
     6661 ctctggaaaa ggatatcgat aatttacaaa aagaaaataa agaattaaaa gacaaaaaag
     6721 aaaagcttca acaagaaaaa gaaaaattag cagataagca aaaagacctt gaaaaagaag
     6781 tgaaagattt aaaaccttca aaagaagata acaaggatga taaaaaagac gaagacaaaa
     6841 ataaagacaa agataaagat aaagaggcat cacaagataa gcaatcaaaa gatcaaacta
     6901 agtcatcgga taaagataat cacaaaaagc ctacatcaac agataaagat caaaaagcta
     6961 atgacaaaca ccaatcataa tcgtattgct tacttgttat agatgaaagg tacagcgttt
     7021 taaaccttat tttaagggta tgtattaatt aaaatgtggt catgattgaa acagaacgta
     7081 aaaatagaca acgtaattaa taaaggagag aaacggcatg catgaacaag attttagaat
     7141 tttagagggt caagatatta ctttgccaga attaggtaga gaattagaaa atattacagg
     7201 acatacgatt gctgattcta ctggcgaaat taagcgtgta attgcacatt taccaaactt
     7261 tgagtccgat acagatactt ttgttgctac atatcgttta aaccatcaac aagattttat
     7321 agatgcaact tttactgcgc tgaaatcaga tagagcacgt ttaaaagaag tgccagttca
     7381 tgttgaactt ataagttata tttctaaatc aaaataaact gctatctaaa acgcaaagtt
     7441 gatcaaaata tcgattttgt gttttttatt gagaaattat ataggagtgt caatcgatga
     7501 tttattgtga aacagagcgt ttaatattaa gagactggca tgaagatgat ctgttacctt
     7561 ttcaaaaaat gaatgcgaat tatgacgtac gtaaatattt tccaagttta ttgagttatc
     7621 gtcgttcaga attagatatg agaactatgg atgcggttat taaagattat ggcattggat
     7681 tatttgctgt agaagataaa gagtcccatc aatggatagg ctttataggt ttgaattata
     7741 ttccagaaac aagcgattat ccatttaaag aattaccgct ttatgaaata ggttggcgct
     7801 tgttgcctga attttgggga aaaggattag caactgaagg cgcaaaggca acgttgaagt
     7861 tagcagaaga acatcaaata tacgatgtct atagttttac agcagaagca aataaagcct
     7921 cacaacgtgt aatggaaaaa attggcatga cagtgtatga tcatttcgaa ttacccaatc
     7981 taagtaagta tcatttatta aaaaggcaag tgcgctatta cattaatctt cgaaagtgaa
     8041 aaatt
//
LOCUS       DAOBBH010000113         8034 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.113, whole genome shotgun sequence.
ACCESSION   DAOBBH010000113 DAOBBH010000000
VERSION     DAOBBH010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8034
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.113"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..1263
                     /locus_tag="P6L01_001806"
     CDS             <1..1263
                     /locus_tag="P6L01_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDP3030872.1"
                     /translation="FSTTIIGALLLYLPFTGKKPISFLDALFIASSAFTVTGLSPVDI
                     GSQFNILGEIVILLLIQIGGLGIVTVTLLTLVFLNRKISMKNRFLIMVTWNIDEPGGV
                     IKLIKHLAIYSLVTELIGMICLCLSFIPKFGIGKGLFLSLFTSVSAFNNAGFALFKNN
                     LIDYSSDPIVIITISILIIFGGIGHFVVIDFINCKKLSKLSLHSKLVLTTTSILIIIG
                     AITFFLLEQFNTMQHMGLVEKIGNSFFQSVTTRTAGFNSIDIASINKSTALMLMLLMF
                     IGGAPLSAAGGIKITTFAVAFIFVLNYIRKENNVSVFNKEISDKHINLSIVTINISFL
                     FISIITFILSIINPNISLIKLLFEVVSAFGTVGLSMNLTTEYHGITKIIIIFVMLCGK
                     VGLLTLLRTFIPPKSPKNYRYTKGQIYL"
     gene            complement(1803..3419)
                     /gene="groL"
                     /locus_tag="P6L01_001807"
     CDS             complement(1803..3419)
                     /gene="groL"
                     /locus_tag="P6L01_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDP3030873.1"
                     /translation="MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFT
                     APLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEG
                     LKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYI
                     SEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYI
                     LVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKA
                     PGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGD
                     GDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKL
                     RIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQ
                     IAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEAGIVDPTKVTRSALQHAASVA
                     AMFLTTEAVVASIPEKNNDQPNMGGMPGMM"
     gene            complement(3495..3779)
                     /gene="groES"
                     /locus_tag="P6L01_001808"
     CDS             complement(3495..3779)
                     /gene="groES"
                     /locus_tag="P6L01_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDP3030874.1"
                     /translation="MLKPIGNRVIIEKKEQEQTTKSGIVLTDSAKEKSNEGVIVAVGT
                     GRLLNDGTRVTPEVKEGDRVVFQQYAGTEVKRDNETYLVLNEEDILAVIE"
     gene            3954..4697
                     /locus_tag="P6L01_001809"
     CDS             3954..4697
                     /locus_tag="P6L01_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDP3030875.1"
                     /translation="MTRLWASLLTVIIYILSQFLPLLIVKKLPFVQYSGIELTKAVIY
                     IQLVLFLIAATTIILINLKIKNPTKLELEVKEPKKYIIPWALLGFALVMIYQMVVSIV
                     LTQIYGGQQVSPNTEKLIIIARKIPIFIFFVSIIGPLLEEYVFRKVIFGELFNAIKGN
                     RIVAFIIATTVSSLIFALAHNDFKFIPVYFGMGVIFSLAYVWTKRLAVPIIIHMLQNG
                     FVVIFQLLNPEALKKATEQANFIYHIFIP"
     gene            complement(4722..5981)
                     /locus_tag="P6L01_001810"
     CDS             complement(4722..5981)
                     /locus_tag="P6L01_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030876.1"
                     /translation="MSYHWFKKMLLSTSILILSSSSLGLATHTVEAKDNLNGEKPTTN
                     LNHNITSPSVNSEMNNNETGTPHESNQTGNEGTGSNSRDANPDSNNVKPDSNNQNPST
                     DSKPDPNNQNPSPNPKPDPDNPKPKPDPKPDPDKPKPNLDPKPDPDNPKPKPDPKPDP
                     DKPKPNPDPKPDPDKPKPNPNPKPDPNKPNPNPSPDPDQPGDSNHSGGSKNGGTWNPN
                     ASDGSNQGQWQPNGNQGNSQNPTGNDFVSQRFLALANGAYKYNPYILNQINKLGKDYG
                     EVTDEDIYNIIRKQNFSGNAYLNGLQQQSNYFRFQYFNPLKSERYYRNLDEQVLALIT
                     GEIGSMPDLKKPEDKPDSKQRSFEPHEKDDFTVVKKQEDNKKSASTAYSKSWLAIVCS
                     MMVVFSIMLFLFVKRNKKKNKNESQRR"
     gene            6178..6804
                     /locus_tag="P6L01_001811"
     CDS             6178..6804
                     /locus_tag="P6L01_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDP3030877.1"
                     /translation="MGLFKKDKKAMTFDNAMEERRSIYNLKDSISISDDELESMIAHA
                     VKHVPSSFNSQSTRIVLLLNDNNNKFWDNTKAILKEVMGENRDFEPTEQKIDNFKHSY
                     GTILFYEDQDVVSGLQEQMPNYYDNFAIWSTQTNAMHQFAIWTALATKGIGASLQHYN
                     PLVDEMTSKEFNIPKSWKLIAQMPFGDIREAAGEKTFNPVEDRFVIKK"
     gene            7165..7950
                     /locus_tag="P6L01_001812"
     CDS             7165..7950
                     /locus_tag="P6L01_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HDP3030878.1"
                     /translation="MKVQIYQLPIIFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWN
                     NGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVAGSVSNIRNYQIFNTAFSVNKSG
                     QLINEYDKVHLVPMLREHEFLTAGENVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
                     SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINP
                     NGDLVGELNESADILTVDLNLNEVEQQRENIPVFKSIKLDLYK"
BASE COUNT         2525 a         1358 c         1235 g         2916 t
ORIGIN      
        1 ttctctacaa ctatcatagg tgctctttta ttatatttac ctttcactgg taaaaagcca
       61 atatcttttt tagatgccct atttatagct tcaagtgcat ttacagttac tggcctatcc
      121 ccagttgata taggatcaca gtttaatata cttggtgaaa tagtaatact attattaatt
      181 caaataggtg gtctgggtat cgtgaccgta accctattga cactagtatt tttaaataga
      241 aagatatcaa tgaaaaatag attcttgatt atggttacat ggaatattga cgaacctggt
      301 ggtgttatta agctaattaa acacttggct atttatagtt tagtcactga attaattggt
      361 atgatttgtt tgtgtttatc ttttatacca aaatttggta taggcaaagg tttattttta
      421 agcttattca catcagtatc agcttttaat aatgctggat ttgccctttt taagaataac
      481 ttaatagatt attctagtga tccaattgtc attattacaa tctcaatact cataatattt
      541 ggaggtattg gacattttgt cgtaatagac tttattaatt gtaaaaaatt gagtaaatta
      601 tctttacatt ctaaattagt cttaactaca actagtatcc taataattat aggagctatt
      661 acattctttt tattagaaca gtttaatact atgcaacata tgggactagt tgaaaaaatc
      721 ggaaattctt ttttccaatc agtaacaaca cgaacagcgg gttttaacag tattgatata
      781 gcaagcatta acaaatctac cgccttaatg ttaatgttac ttatgtttat tggtggtgcc
      841 cctctcagtg cagctggagg aattaaaata actacttttg cagtggcgtt tatatttgtt
      901 ttaaattata tacgtaaaga aaataatgtt tcagtattca ataaagaaat atctgacaaa
      961 catataaatc tatctattgt taccattaat atctcatttc tatttatcag tatcattact
     1021 tttatattat cgataattaa tccgaacata tcattaatca agttattatt cgaagtggtt
     1081 tctgcattcg gaacagtcgg gttaagtatg aaccttacca cagaatatca tggtattact
     1141 aaaataatta ttatattcgt tatgctttgc ggtaaagtag gactattaac tttattaaga
     1201 acatttatac caccaaaaag tcctaaaaat taccgctaca ctaaaggaca aatttatcta
     1261 taaaataact atccatcttt aacaagaaat gaactagtgt tttaatctat tacatttaaa
     1321 aaactaccta aacaggtagt tttttgtttt ttatttattt taggcccatt aaatgtctta
     1381 tctctttaat taagtaaaat gcacaagtac acctttaaaa agatttactc aaaactaaac
     1441 tatacctagt taaccgcttg tgattaagaa attttaaatg tggtttcttt taattatgtc
     1501 aaccacccat aaggatttta atcaagcact cattcattcc tgcactcgca atatgcatta
     1561 cttatagaag tggatgcact ctcatattat ggcaatacca taactcccgc accataatga
     1621 tttaaatacc attatgaatc tcaattacaa attagcacat aaaaaagacg cctctcataa
     1681 gtttagtcaa ctaatggaag gtgtcttaaa ttaaaatatt ttcatacgct ctaatattta
     1741 tttggaacga atcatactcg tctatcatgg aatgttctag ttagtcaaaa ctagaaactg
     1801 ttttacatca ttcccggcat gccacccatg ttaggttggt cattattttt ttctggaatt
     1861 gatgctacaa ccgcttcagt cgttaagaac attgctgcaa cacttgcagc atgttgtaat
     1921 gctgagcgtg ttactttagt tggatcaacg atacctgctt ctaacatatt aacccactcg
     1981 tttgtagcag cgttaaaacc aacacccggc tctgcgtttt tcaaacgttc tacaataaca
     2041 gaaccttcta atcctgcatt ttcagcaatt tgacgaactg gtgcagttag tgctttaagt
     2101 acaatattta cacctgtttc aatgtcacct tctgcttcaa tttcacttac tttttggtaa
     2161 acatttacta atgcagtacc accacctgca acaatacctt cttcaactgc tgcacgtgta
     2221 gaatttaatg catcttcaat acgtaattta cgttctttaa gctctgtttc acttgctgca
     2281 cctactttaa taactgcaac accacctgct aatttagcta agcgctcttg taatttttca
     2341 cgatcaaagt cagattcagt ttcttcaatt tgagatttca attggcttac acgtgcatca
     2401 atgctgtttt cgtcaccgtc accatcaaca acagtggtat tatctttagt tacttctact
     2461 ttacttgcag tacctaacat atcaattgat gcatctttta aatctaagcc taaatcatca
     2521 gtaatcactt gcgcaccagt taaaatagct aaatcttcta acattgcttt acgacgatca
     2581 ccgaaaccag gcgcctttac tgcaacagct gtaaatgtgc cacgcattcg gtttagcacg
     2641 atatttgtta atgcatcgcc ttcaacttca tcagctacaa ttaagattgg acgattagat
     2701 tgaaccactt gttctaataa aggtaagata tcttggaaag acgagatttt cttatctgtt
     2761 actaaaatgt atgggcgttc taattcagca accattttat ctgaatcagt aaccatatat
     2821 ggtgattgat aaccacgatc aaattgcata ccttcaacca cttctagttc agtgtttagt
     2881 ccatttgatt cttcaattgt aatgacacca tcgttaccta ctttttccat agcttcagaa
     2941 atataacgtc caatttcttc atctgctgct gaaatcgcac ctacttgcgc tatttcattt
     3001 ttattttcaa ctttttgaga attttcatgt aatgcttcta cagcaacttt aactgctttg
     3061 tcgatacctt gacgtaaacc aactgggttc gcaccacttg taacattttt caagccttct
     3121 tgaatcattg cttgagctaa tactgttgca gttgtcgtac cgtcaccagc aatttcattt
     3181 gtcttatttg caacttcttg aactagttta gcccccatat tttcatatgg atcttctaat
     3241 tcgatttctt tagcaatcgt tacaccatcg ttcgtaatta aaggtgctgt aaactcttta
     3301 tctaatacaa cattacgtcc tttaggacca atcgttactt taactgcatt tgctaattgg
     3361 tctacaccac gtaacattgc ttgacgtgca tcttcagaga atttcaattg tttaaccata
     3421 aatgataaac ctccatttgt ttagtcatat ttcattttcg ttctttacaa tttatctatg
     3481 aatttaattt tatattattc aattaccgct aaaatatctt cttcatttaa tactagatat
     3541 gtttcattat ctcgtttaac ttctgtacca gcatattgtt ggaacacgac acggtcccct
     3601 tctttcactt caggagtcac tcttgtacca tcatttaata gacgtccagt tcctactgca
     3661 acgataacgc cttcgtttga tttttcttta gcactatcag ttaaaacaat accactttta
     3721 gttgtttgtt cttgttcttt tttctcaata atcacacgat ttccaattgg ttttagcatg
     3781 attgttcctc cttaaaaaac ctaaagttta gcacttaaca ttaaagagtg ctaacataca
     3841 tttataataa tcaaatttgg tcaaaatttc aagtcataac cttttaatta agttttgtaa
     3901 cttcaagtta tttacgataa aataaattat aaacaaatat ttggaggaaa attatgacaa
     3961 gattatgggc atcattgcta actgttatta tttatatatt gtctcaattt ttaccgcttc
     4021 tcattgtaaa aaaattacca tttgtacaat atagtggcat agaactgact aaagcagtca
     4081 tttacataca acttgttcta tttttaatcg ccgccacgac gattatttta attaatttaa
     4141 aaattaaaaa tccaacaaaa ttagaattag aagttaaaga acctaaaaaa tatatcattc
     4201 catgggcatt gcttggattt gcattggtaa tgatttatca aatggtagtg agcattgtat
     4261 taacgcaaat ttatggtgga caacaagtaa gtcctaatac agaaaagcta attattattg
     4321 ctcgaaaaat acctatattt atcttctttg tatctattat cggtccttta ttagaagaat
     4381 acgtattcag aaaagtaatc tttggagaat tatttaatgc gattaaaggt aatcgtatcg
     4441 tagcatttat tattgctaca acagtaagtt cattaatatt tgcattagca cataatgatt
     4501 tcaaatttat tccagtttat tttggtatgg gcgtcatttt ttcattagca tatgtatgga
     4561 caaaacggct tgctgttcca attattatcc atatgttaca aaacggattt gtcgttatat
     4621 tccaattact taatcctgaa gctttgaaaa aagccacgga acaagcgaat tttatatatc
     4681 acatttttat tccataatat aaaaaacgaa tcacacacgg attatcgtcg ctgtgattcg
     4741 tttttatttt tctttttatt tcgctttaca aataagaata gcatgattga aaataccacc
     4801 atcatagaac atacaattgc tagccaactt ttactatatg cagttgacgc acttttctta
     4861 ttatcttctt gttttttaac tactgtaaaa tcgtcttttt catgcggttc aaatgagcgt
     4921 tgttttgaat ccggcttatc ttcgggcttt ttcaaatctg gcattgatcc aatttcacca
     4981 gtaattaatg cgagtacttg ttcatctaaa ttacgatagt acctttctga tttcaatgga
     5041 ttgaaatatt ggaatctaaa gtaattcgat tgctgttgta atccatttaa atatgcattt
     5101 ccgctgaaat tttgttttcg aataatatta taaatgtctt catcagtaac ttctccataa
     5161 tctttgccca acttattaat ttgatttaaa atatacggat tatacttgta agccccattt
     5221 gccaaggcta aaaatcgttg ggatacaaaa tcattaccag taggattttg tgagtttcct
     5281 tgattcccat ttggttgcca ttgaccttga ttagatccat ctgaagcatt tgggttccat
     5341 gttcccccat ttttcgagcc accagaatga tttgaatccc caggttgatc gggatctggt
     5401 gacgggttag gatttggctt attagggtct ggttttggat tcggatttgg ctttggttta
     5461 tctggatctg gttttggatc cggatttggc tttggtttat ctgggtctgg ttttggatct
     5521 ggttttggtt tcgggttatc tggatctggt tttggatcca gatttggctt tggtttatct
     5581 gggtctggtt ttggatccgg ttttggtttc gggttatctg gatctggttt tggattcgga
     5641 cttgggtttt ggttatttgg gtctggtttt gaatctgtac ttgggttttg gttgtttgag
     5701 tctggcttca cattattcga atcaggatta gcatcacgac tattcgaacc tgttccttca
     5761 ttacccgttt gatttgattc gtgaggtgtc ccagtctcat tattattcat ttcactattt
     5821 actgatggtg aagttatatt atgattcaaa ttagtagttg gtttttctcc atttaagtta
     5881 tcctttgctt caactgtgtg cgttgcaagc cctaaactac tactacttaa aattaaaata
     5941 cttgttgaaa gtaacatttt cttaaaccaa tgatatgaca tttcacgaga cacctccctt
     6001 tttcatgtaa gtattaatct aagtataata aatatgcgtt gaaaatgcat ttatatttgg
     6061 aatttgaaaa tgaatattaa tattcttgaa attttcgtaa atattacttt acaagtccga
     6121 gtataaattt tatacttagt ctattaagta ttctttacta aagaaggagc aataattatg
     6181 ggcttattta aaaaagataa aaaagcaatg acttttgaca atgcaatgga agaaagacgt
     6241 tcaatatata atttgaaaga ttcgatatca attagcgatg acgaattaga atcaatgatt
     6301 gcacatgcag ttaaacatgt tccttcatca tttaattctc aatcaactag aattgttctc
     6361 ttgttaaatg acaacaataa taaattttgg gataacacaa aagcaatttt aaaagaagtc
     6421 atgggagaga atcgtgattt cgagccaact gaacaaaaaa ttgataactt caaacattca
     6481 tacggtacaa ttttgtttta tgaagatcaa gatgttgtaa gtggtttaca agaacaaatg
     6541 ccaaactact atgataattt tgcaatatgg tcaactcaaa caaatgccat gcatcaattt
     6601 gcaatttgga ctgcattagc tactaaaggc ataggtgctt cattacaaca ctataatcca
     6661 cttgtcgatg agatgacttc aaaagaattt aatattccta aaagttggaa acttattgct
     6721 caaatgccat ttggtgatat tcgtgaagct gctggcgaaa aaacatttaa tccagtggaa
     6781 gatcgtttcg taataaaaaa ataatcaata tattgttaag aatgggacag aaatgataaa
     6841 gaatcattag ttattcatta tgtagtagtt cttacacatt agccgctgct aatgtgcact
     6901 taagaatagg aatacatgag taaaactcat gtataagaaa tactaatttc taaagaaaaa
     6961 gtatttcttt atgttagagc ccaccccaac ttgcattgtc tgtagaaatt ggtgttccaa
     7021 tttctctatg ttggggcccc atcaactact gtcaatataa cattgtagag cctaggactt
     7081 tgattttatg tcttgggctc gttttttgcg tttcaacata acttttagta agttatatat
     7141 aagatctatc aaggatgcga tgttatgaaa gtccaaattt atcaattacc gattattttt
     7201 ggagatagtt ctaaaaatga aactcaaata acgcaatggt tcgaaaaaaa tatgaatgct
     7261 gaagtagatg tagtcgtttt acctgaaatg tggaataatg gctatgattt agagcattta
     7321 aatgaaaaag cagataataa tctaggacaa agtttttcat ttattaaaca tttagctgaa
     7381 aaatataaag tcgatatcgt agctgggtca gtttctaaca ttagaaacta tcaaatattt
     7441 aatacagctt ttagtgtgaa taaaagtggc cagctgatta atgaatacga caaagttcat
     7501 ttagttccta tgttaagaga gcatgaattt ttaaccgctg gcgaaaacgt agctgaaccc
     7561 tttcaattgt ctgacggtac ttatgtgacc caacttatct gttatgattt acgttttcca
     7621 gaattattaa gatatcctgc tcgtagtggt gctaaaattg cattttatgt ggcgcaatgg
     7681 cctatgtcac gtcttcaaca ttggcattca ttactaaagg cacgtgcaat tgaaaataat
     7741 atgtttgtca ttggaactaa tagcactgga ttcgatggta acacagaata tgctggtcat
     7801 tcgatagtta taaacccaaa tggagattta gttggagaat taaatgaatc cgcagatatt
     7861 ttgactgtag atttaaactt aaatgaagta gaacagcaac gcgaaaatat acctgtattt
     7921 aaaagtatta aattagattt gtataaataa gaagcgcccg aaataatatt taacacatgt
     7981 catgactaaa catagattta tgagagattt ttcataagtc tatgtttatt tttt
//
LOCUS       DAOBBH010000114         7923 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.114, whole genome shotgun sequence.
ACCESSION   DAOBBH010000114 DAOBBH010000000
VERSION     DAOBBH010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7923
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.114"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..119)
                     /locus_tag="P6L01_001813"
     CDS             complement(<1..119)
                     /locus_tag="P6L01_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDP3030879.1"
                     /translation="MFKKLINKKNTINNYNEELDSSNIPEHIAIIMDGNGRWA"
     gene            complement(492..1046)
                     /gene="frr"
                     /locus_tag="P6L01_001814"
     CDS             complement(492..1046)
                     /gene="frr"
                     /locus_tag="P6L01_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDP3030880.1"
                     /translation="MSDIINETKSRMQKSIESLSRELANISAGRANSNLLNGVTVDYY
                     GAPTPVQQLASINVPEARLLVISPYDKTSVADIEKAIIAANLGVNPTSDGEVIRIAVP
                     ALTEERRKERVKDVKKIGEEAKVSVRNIRRDMNDQLKKDEKNGDITEDELRSGTEDVQ
                     KATDNSIKEIDQMIADKEKDIMSV"
     gene            complement(1065..1787)
                     /locus_tag="P6L01_001815"
     CDS             complement(1065..1787)
                     /locus_tag="P6L01_001815"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDP3030881.1"
                     /translation="MAQISKYKRVVLKLSGEALAGEKGFGINPVIIKSVAEQVAEVAK
                     MDCEIAVIVGGGNIWRGKTGSDLGMDRGTADYMGMLATVMNALALQDSLEQLDCDTRV
                     LTSIEMKQVAEPYIRRRAIRHLEKKRVVIFAAGIGNPYFSTDTTAALRAAEVEADVIL
                     MGKNNVDGVYSADPKVNKDAVKYEHLTHIQMLQEGLQVMDSTASSFCMDNNIPLTVFS
                     IMEEGNIKRAVMGEKIGTLITK"
     gene            complement(1924..2805)
                     /locus_tag="P6L01_001816"
     CDS             complement(1924..2805)
                     /locus_tag="P6L01_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDP3030882.1"
                     /translation="MATISAKLVKELRKKTGAGMMDCKKALTETDGDIDKAIDYLREK
                     GIAKAAKKADRIAAEGLVHVETKGNDAVIVEINSETDFVARNEGFQELVKEIANQILD
                     TKAETVEALMETTLPNGKSVDERIKEAISTIGEKLSVRRFAIRTKTDNDAFGAYLHMG
                     GRIGVLTVVEGSTDEEAARDVAMHIAAINPKYVSSEQVSEEEINHEREVLKQQALNEG
                     KPENIVEKMVEGRLRKYLQEICAVDQDFVKNPDVTVEAFLKTKGGKLVDFVRYEVGEG
                     MEKREENFADEVKGQMK"
     gene            complement(2840..2953)
                     /locus_tag="P6L01_001817"
     CDS             complement(2840..2953)
                     /locus_tag="P6L01_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030883.1"
                     /translation="MGDKILMLITFFKKKIEANYKYSIRVLAILPIIMLKS"
     gene            complement(2987..3754)
                     /gene="rpsB"
                     /locus_tag="P6L01_001818"
     CDS             complement(2987..3754)
                     /gene="rpsB"
                     /locus_tag="P6L01_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDP3030884.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKKVDEAYNFLKQVSEDGGQVLFVGTKKQAQESVKSEAERAGQFYINQRWLGGLLT
                     NYKTISKRIKRISEIEKMEEDGLFEVLPKKEVVELKKEYDRLIKFLGGIRDMKSMPQA
                     LFVVDPRKERNAIAEARKLNIPIVGIVDTNCDPDEIDYVIPANDDAIRAVKLLTAKMA
                     DAILEGQQGVSNEEVAAEQNIDLDEKEKSEETEATEE"
     gene            complement(3953..4027)
                     /locus_tag="P6L01_001819"
     CDS             complement(3953..4027)
                     /locus_tag="P6L01_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030885.1"
                     /translation="MTLMIMLKSFLYFCLIFNQIDISH"
     gene            complement(4101..4874)
                     /gene="codY"
                     /locus_tag="P6L01_001820"
     CDS             complement(4101..4874)
                     /gene="codY"
                     /locus_tag="P6L01_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDP3030886.1"
                     /translation="MSLLSKTRELNTLLQKHKGIAVDFKDVAQTISSVTVTNVFIVSR
                     RGKILGSSLNELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPEN
                     RELFIDSRTTIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHS
                     EVEKEARDKAAITMAINSLSYSEKEAIEHIFEELGGTEGLLIASKVADRVGITRSVIV
                     NALRKLESAGVIESRSLGMKGTFIKVKKEKFLDELEKSK"
     gene            complement(4899..6302)
                     /gene="hslU"
                     /locus_tag="P6L01_001821"
     CDS             complement(4899..6302)
                     /gene="hslU"
                     /locus_tag="P6L01_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDP3030887.1"
                     /translation="MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLL
                     DEESKQEISPKNILMIGPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVES
                     MVRDLVDVSVRLVKAQKKSLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGG
                     AIPNFGQNNEDEEEPPTEEIKTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTN
                     QNQQMQEMMNQLMPKKKVEREVAVETARKILADSYADELIDQESANQEALELAEQMGI
                     IFIDEIDKVATNNHNSGQDVSRQGVQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAF
                     HVSKPSDLIPELQGRFPIRVELDSLSVEDFVRILTEPKLSLIKQYEALLQTEEVTVNF
                     TDEAITRLAEIAYQVNQDTDNIGARRLHTILEKMLEDLSFEAPSMPNAVVDITPQYVD
                     DKLKSISTNKDLSAFIL"
     gene            complement(6368..6913)
                     /gene="hslV"
                     /locus_tag="P6L01_001822"
     CDS             complement(6368..6913)
                     /gene="hslV"
                     /locus_tag="P6L01_001822"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDP3030888.1"
                     /translation="MSNTTLHATTIYAVRHNGKAAMAGDGQVTLGQQVIMKQTARKVR
                     RLYEGKVLAGFAGSVADAFTLFEKFETKLQQFSGNLERAAVELAQEWRGDKQLRQLEA
                     MLIVMDKDAILVVSGTGEVIAPDDDLIAIGSGGNYALSAGRALKRHASHLSAEEMAYE
                     SLKVAADICVFTNDNIVVETL"
     gene            complement(6910..7806)
                     /gene="xerC"
                     /locus_tag="P6L01_001823"
     CDS             complement(6910..7806)
                     /gene="xerC"
                     /locus_tag="P6L01_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDP3030889.1"
                     /translation="MNHIQEAFLNTLKVERNFSEHTLKSYQDDLIQFNQFLEQEHLQL
                     KTFEYRDARNYLSYLYSNHLKRTSVSRKISTLRTFYEYWMTLDENIINPFVQLVHPKK
                     EKYLPQFFYEEEMEALFKTVEEDTSKNLRDRVILELLYATGIRVSELVNIKKQDIDFY
                     ANGVTVLGKGSKERFVPFGAYCRQSIENYLEHFKPIQSCNHDFLILNMKGEAITERGV
                     RYVLNDIVKRTAGVSEIHPHKLRHTFATHLLNQGADLRTVQSLLGHVNLSTTGKYTHV
                     SNQQLRKVYLNAHPRAKKENET"
BASE COUNT         2262 a         1563 c         1106 g         2992 t
ORIGIN      
        1 ttagcccatc gcccattacc atccataata atagcgatat gttcaggtat attagacgag
       61 tctaattctt cattataatt atttatagtg ttctttttat ttattagctt tttaaacatg
      121 gtctttcctc cgagcctgat catctctgtc atataatata agttactaaa caatcattat
      181 cattttatca tactcattta atgcattgaa taagcaaata aaaaaactgt accaaagtct
      241 gactcggtac agtttacacc agaaatgtga catacatact gaggttaaaa attacataac
      301 tttattatag caatatacct aatgcctatt atttataaag tatatgttta attaacttgt
      361 ttaaacacca tttttatgct cattgtgaat ttaaattatc acatgcatct ttgatttaat
      421 taaacatgcc cattttaaat gttacattat cgtttaatac tggcatttta atatgtcatt
      481 gtatattagt tttatactga cataatatct ttttctttat cagcaatcat ttgatcaatt
      541 tcttttattg aattgtctgt tgctttctga acatcttcag taccacttct caactcatct
      601 tcagtaatgt cgccattttt ttcatctttt ttcaactgat cattcatatc acgacgaata
      661 tttcgaacag atactttagc ttcttcacca attttcttaa catctttaac gcgctctttt
      721 ctacgctctt ctgttaaggc aggtactgca atacgtatca cttcaccatc acttgttggg
      781 ttgacaccta agttagccgc tattatcgct ttttcgatgt cagctacaga agttttgtcg
      841 tatggagaaa taacaagtaa acgtgcttct ggaacattga tgcttgctaa ttgttgtaca
      901 ggtgttggtg caccatagta atcaactgtt acgccgttta ataaatttga attagctctt
      961 cctgcactga tattagctaa ttcacgtgat aagctttcga ttgatttttg cattcttgat
     1021 ttagtttcat taataatgtc actcattatt ttacacctct aaatttattt tgtaattaac
     1081 gtacctatct tttcacccat aacagcacgt ttaatattcc cttcttccat aatagagaaa
     1141 acagttaacg gaatgttatt atccatacag aatgaggatg ctgttgaatc cattacttgt
     1201 aaaccttctt gaagcatttg aatatgcgtt aaatgttcat attttaccgc atctttgttt
     1261 actttaggat ctgcagaata tacaccatct acattatttt tgcccattaa aataacatct
     1321 gcttcaactt ctgcagcacg taatgccgct gtagtatctg tagagaagta tgggtttcca
     1381 atacctgcag caaaaataac tacgcgtttc ttttctaagt gtctaattgc acgacgacga
     1441 atataaggtt cagccacttg cttcatttca atagatgtta atactcgtgt atcacaatcc
     1501 aattgttcta aactatcttg taatgctaag gcattcatta cagttgcaag catacccatg
     1561 taatcagcag ttccacggtc catacctaag tcactacctg ttttacctct ccaaatgttt
     1621 ccgccaccaa cgattactgc gatttcacag tccattttag caacttcagc cacttgctca
     1681 gcaacacttt taataattac tggatttatg ccaaatcctt tttctccagc taacgcttca
     1741 ccacttagtt tcaaaactac acgtttatat ttagaaattt gagccatttt cttatcctct
     1801 ctatcgtgaa tatatgtaac atatacaagt aaagaagaca cagttgtcgt tttattagaa
     1861 tgatttacat tttaataaag caacattaaa ggtgtcttct tccttgtttt actttatgac
     1921 agattatttc atttgtcctt taacttcatc cgcaaagttt tcttcgcgtt tttccatacc
     1981 ttcgcctact tcatagcgta cgaagtcaac aagttttcca ccttttgttt ttaagaaagc
     2041 ttcaactgtt acatcagggt ttttaacgaa gtcttgatct acagcacaaa tttcttgtaa
     2101 gtatttacgt aaacgtcctt ccaccatttt ttcaacgatg ttttctggtt taccttcatt
     2161 taatgcttgt tgttttaaaa cttctctttc gtggttgatt tcttcttcgc taacttgttc
     2221 agaagaaaca tatttagggt tgattgcagc gatatgcata gcaacgtctc ttgctgcttc
     2281 ttcgtcagtt gaaccttcaa caactgttaa tacaccaatg cgtccaccca tgtgtaagta
     2341 agcgccgaaa gcatcgttat cagttttagt tctgatagca aaacgacgaa cacttaattt
     2401 ttcaccgatt gttgaaattg cttctttaat tctttcatca actgatttac catttggtaa
     2461 agttgtttcc attaaagctt caacagtttc agcttttgta tctaatattt gattagcgat
     2521 ttctttaact aactcttgga atccttcgtt acgagcaaca aagtctgttt cagagttgat
     2581 ttcaacgata actgcgtcgt tacctttagt ttctacatgt actaaacctt cagccgcaat
     2641 acggtctgct tttttagctg ctttagcaat acctttttca cgtaggtaat caatcgcttt
     2701 atcgatgtca ccatcagttt cagttagcgc ttttttacaa tccatcatac ccgcgccagt
     2761 ttttttacgt aattctttaa caagttttgc tgaaatagtt gccattcatt attcctccaa
     2821 taagttatca ttttatatat tatgatttta gcattattat aggcaagatt gccaatactc
     2881 taattgaata tttgtaattt gcctcgattt tctttttaaa aaaggtgata agcattaata
     2941 tcttatcacc catttaaact atatctaagt cagattcaac agttgattat tcttcagttg
     3001 cttctgtttc ttctgatttt tctttttcat ctaaatcgat gttttgttct gcagctactt
     3061 cttcattaga aacgccttgt tgaccttcta agattgcatc tgccatttta gcagttaata
     3121 atttaaccgc acggatagca tcgtcgtttg ctgggataac gtagtcaatt tcgtcaggat
     3181 cacagttagt gtcaacgata cctacgatag gaatatttaa tttacgagct tcagcaattg
     3241 cattacgctc tttacgtggg tcaactacga ataatgcttg aggcattgat ttcatatcac
     3301 gaattccgcc taagaatttg attaaacggt cgtattcttt tttaagttct actacttctt
     3361 ttttaggtaa tacttcgaat aaaccatctt cttccatttt ttcaatttca gaaatacgtt
     3421 tgattcgttt tgagattgtt ttatagttag ttaataatcc acctaaccat ctttggttaa
     3481 tgtagaattg accagcacgt tctgcttcag atttaactga ttcttgtgct tgttttttag
     3541 ttcctacgaa taagacttgt ccaccatctt ctgaaacttg tttcaagaag ttgtatgcct
     3601 cgtctacttt tttcactgtt ttttgtaagt cgatgatata aataccattt ctctcagtga
     3661 agatatattt tttcattttt gggttccaac gacgtgtttg gtgaccgaag tgaacacccg
     3721 cttctagtaa ttgtttcatt gaaattactg ccattataaa ttcctcctat tggttaatta
     3781 cctccatagc aaactattgt aaagacaata tataaatatt gcacccttta cacaatcacg
     3841 tttgatatgt gtgtattggc tttatagccg aaaataaata taacataatg ccgtgtctaa
     3901 agcaaatgtt tttcaagcat ttttccataa atttaaaggt aattctatca agttaatgtg
     3961 aaatatcaat ttgattgaaa attagacaaa aataaagaaa tgacttcaac ataatcatca
     4021 aagtcatttc gttgagttga ctactgccaa gataacatcg tcacgactag gacattgaat
     4081 tatgtcccag actcatcgac ttatttactt ttttctaatt catctaagaa tttttctttt
     4141 ttaactttaa tgaaagtacc tttcattcct aaagaacgtg attcaattac accagcactt
     4201 tctaatttac gtagtgcatt tacaattaca gatctagtaa taccaactct atctgcaact
     4261 tttgatgcga ttaataggcc ttccgtaccg ccaagttctt caaagatatg ttcaatcgct
     4321 tctttttcag aataagataa tgaattaatt gccattgtaa tagcagcttt atcgcgcgct
     4381 tctttttcta cttcactatg cttctcacgt aagatttcca taccaataac tgtagcagca
     4441 tattcaccta gtaccaaatc attttcatta aaatcatcat gtactcgacc aagtactaat
     4501 gtacctaatc tttctcctcc acctaaaatt gggaagatag ttgtacgact atctatgaat
     4561 aattctctgt tttcaggtgg gaatactgtt aatacattgt cgatatcaat atttgattct
     4621 gtttgtttaa cttccattaa tcgttctgta tattcacttg gaatatgtct ttcttccaac
     4681 atttgaataa ttctttgact ttttaataat tcatttagac tcgaccctaa aattttacct
     4741 cgacgcgata caataaatac atttgttaca gttacgctac taatcgtttg tgctacatct
     4801 ttaaaatcaa ccgcaatacc tttgtgtttt tgaagtaacg tgtttaactc tctcgtttta
     4861 gataataagc tcatgaattt ttctcctttt gtatattttt atagaataaa tgcacttaaa
     4921 tctttatttg ttgaaattga ttttaattta tcatcaacat attgtggggt aatatctaca
     4981 actgcattcg gcatacttgg tgcttcgaat gataaatctt ctagcatctt ttctaaaatt
     5041 gtatgaagtc gacgtgcacc aatgttgtct gtatcttgat ttacttgata agcaatctca
     5101 gctaagcgag taattgcttc atcggtaaag tttacagtaa cttcttctgt ttgaagcaat
     5161 gcttcatatt gtttaattaa tgacaatttt ggttctgtca aaattcttac aaaatcttct
     5221 accgataaac tatcaagttc aactctaatc ggaaaacgac cttgcaattc tggtatcaag
     5281 tcactcggct tagatacatg gaaagctcca gcacctataa acagcatatg ttcagtattc
     5341 acagtaccat atttggtttg aataacgcta ccttcaagta taggtaaaat atctctttga
     5401 acaccttgtc ttgagacatc ttgaccacta ttatgattat tcgtcgcaac tttgtcgatt
     5461 tcatctataa agatgatacc catttgttct gctaattcaa gcgcttcttg gttagcgctt
     5521 tcttgatcaa ttagttcatc cgcataacta tcagctaaga ttttccttgc cgtctcaaca
     5581 gcaacttctc gctcaacttt ctttttaggc attaattgat tcatcatctc ttgcatttgc
     5641 tgattttgat ttgtacctag catacctaaa gcaccaggat cttgttcgac tttaattctt
     5701 accttttctt tttcaagttt gccttcttct agctgtctct taatttcaga acgttttgtt
     5761 ttaatttcct cagtaggtgg ttcttcttca tcttcgttat tttgtccgaa atttggaatt
     5821 gcacctccga aaagtgactc taaaggatta ttcgtttgag acgctttctt tttcatactt
     5881 ggaactaata acttaacaag tttttcattg gccttagctg ttgcttcatc ttgtaccaat
     5941 gattttttct gcgccttgac taatcttact gaaacatcaa caagatctct aaccatactt
     6001 tcaacatctc gtcctacata acctacctca gtaaatttag tagcttctac ttttataaat
     6061 ggcgcgccga caactttggc cattcttctt gcaatttcag ttttaccaac gccggttggt
     6121 ccaatcatca aaatattttt aggtgaaatt tcttgctttg attcctcatc taataaactt
     6181 cttctgtatc gattacgtag ggcaattgcc actttacgtt tagcatcatt ttgtccaacg
     6241 atatattcat ttaatttaga tacgatttct tttggagtta atcttattcc agctgtatcc
     6301 attaatttca ttcctccgtc ttttaactaa aattaattgg tcgtaattat ttatcgtgct
     6361 ctgattatta tagtgtttca acaacaatat tatcgttggt aaagacacaa atatcagccg
     6421 ctactttcaa gctctcatat gccatttctt cagcagacaa atgcgatgca tggcgtttca
     6481 atgcacgtcc tgcgcttaat gcgtagttgc cccctgatcc aatagcgata aggtcatcat
     6541 ctggggcaat aacttcgcca gttccactga caactaaaat agcatcttta tccattacaa
     6601 ttagcatagc ttctaattga cgtaattgtt tatcgcctcg ccattcttgt gccaattcaa
     6661 cagcagctct ttctaagtta ccactaaact gttgtaattt tgtttcaaat ttttcaaata
     6721 acgtaaacgc atctgctaca ctacctgcga aaccagctaa cactttacct tcatataaac
     6781 gtcgcacttt tcttgccgtt tgtttcatga tgacttgttg accaagcgtt acttgtccat
     6841 ctccagccat agctgctttc ccattatgtc ttacagcata aattgttgtt gcatgtaatg
     6901 ttgtattact catgtttcat tctccttttt cgctcgagga tgtgcattta gatacacttt
     6961 tcttaattgt tggttagata cgtgtgtata tttaccagtt gttgacaaat taacatgacc
     7021 taataacgat tgtactgttc ttaggtctgc accttgattc aataaatgcg ttgcaaatgt
     7081 atgtctgagc ttgtggggat gaatctcact tacgcctgct gttcgtttaa caatatcatt
     7141 taaaacatat cgtacaccgc gttcagtgat tgcttcaccc ttcatattta aaataagaaa
     7201 atcatgattg catgactgaa ttggtttgaa atgttctaaa taattttcga tgctttgtct
     7261 acaataagca ccaaacggta caaaacgctc tttgctccct tttcctaata cggtaacacc
     7321 attcgcgtaa aaatctatat cttgtttttt aatatttact aattccgaaa cacggatgcc
     7381 tgtagcatac aacaattcaa gaataactcg atcccgtaaa ttttttgaag tgtcctcttc
     7441 tacagttttg aataacgctt ccatttcttc ttcgtaaaag aattgcggaa gatatttttc
     7501 ttttttcgga tgtactaatt gaacaaatgg attaataatg ttctcatcaa gcgtcatcca
     7561 atattcatag aaagttctta acgttgagat tttacgagaa acagatgttc ttttcaaatg
     7621 atttgaatat aaatagctca aataatttct agcatctctg tattcaaaag ttttcaactg
     7681 taaatgttct tgttctaaaa attgattaaa ctgaattaag tcatcttgat atgatttcaa
     7741 tgtatgttcc gaaaaattcc gttcaacttt caatgtattt aaaaacgctt cttgaatatg
     7801 attcaataca tttaccctcc atcaataaga attgtagcat atacagcaaa gggcttacag
     7861 taatttgata atcaattcta aatcttttga gaattaaata attgtaacta atcggaaaac
     7921 ctg
//
LOCUS       DAOBBH010000115         7910 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.115, whole genome shotgun sequence.
ACCESSION   DAOBBH010000115 DAOBBH010000000
VERSION     DAOBBH010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7910
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.115"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..1502)
                     /locus_tag="P6L01_001824"
     CDS             complement(<1..1502)
                     /locus_tag="P6L01_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDP3030890.1"
                     /translation="MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFID
                     NGWLPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPM
                     LLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLM
                     KFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQS
                     ILYTIESNPGPGLGILLAYMIFGKGTAKATSYGAGIIHFLGGIHEIYFPYVLMRPLLF
                     IAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFV
                     VAALIMKFTREPKQDLEAATAQMENTKGKKSSVASKLVSSDKNVNTEENASGNVSETS
                     SSDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGIND
                     ITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYE"
     gene            2183..3988
                     /gene="glmS"
                     /locus_tag="P6L01_001825"
     CDS             2183..3988
                     /gene="glmS"
                     /locus_tag="P6L01_001825"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDP3030891.1"
                     /translation="MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTV
                     FKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGV
                     IENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALG
                     LLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKD
                     EVIIKDADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEG
                     NLKIDQDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMP
                     LLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAG
                     PEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIM
                     EQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIE
                     DGTPVVALATQENVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTP
                     LVSVVALQLISYYAALHRDLDVDKPRNLAKSVTVE"
     gene            4220..5002
                     /locus_tag="P6L01_001826"
     CDS             4220..5002
                     /locus_tag="P6L01_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3030892.1"
                     /translation="MLIQLDQIGRMKQGKTILKNISWQIAKGDKWILYGLNGAGKTTL
                     LNILNAYEPATTGGVNLFGKMPGKVGYSAETVRQHIGFVSHSLLEKFQEGERVIDVVI
                     SGAFKSIGVYQDIDDEVRNEAHHLLKLVGISAKAQQYIGYLSTGEKQRVMIARALMGQ
                     PQVLILDEPAAGLDFIARESLLNILDSLSDSYPTLAMIYVTHFIEEITGNFTKILLLK
                     DGESVQQGLIDDILTSENMSRFFQKNVAVQRWNNRFSMAMLE"
     gene            5070..5927
                     /locus_tag="P6L01_001827"
     CDS             5070..5927
                     /locus_tag="P6L01_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDP3030893.1"
                     /translation="MDNVKAIFLDMDGTILHENNQASTYTKDVINQLREKGYKVFLAT
                     GRSHSEIHQLVPQDFAVNGIISSNGTIGEVDGEIIFKHGLSLAQVQQITNLAKRQQIY
                     YEVFPFEGNRVSLKEDETWMRDMIRSQDPINGVSHSEWSSRQDALAGKIDWVTKFPEG
                     EYSKIYLFSSNLEKITAFRDELKQNHVQLQISVSNSSRFNAETMAYQTDKGTGIKEMI
                     AHFGIHQEETLVIGDSDNDRAMFEFGHYTVAMKNARPEIQALTSDVTAYTNEEDGAAK
                     YLAKHFLAD"
     gene            complement(6585..6743)
                     /locus_tag="P6L01_001828"
     CDS             complement(6585..6743)
                     /locus_tag="P6L01_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDP3030894.1"
                     /translation="MSKSSDALFNSLTQSFELIEGVYNELVTDNLKIVMYKPRKESTS
                     GRINRRFK"
     gene            7423..7527
                     /locus_tag="P6L01_001829"
     CDS             7423..7527
                     /locus_tag="P6L01_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030895.1"
                     /translation="MLLLERTSMSDFEMLMVVLTIIGLVLISTQDHKK"
BASE COUNT         2730 a         1177 c         1442 g         2561 t
ORIGIN      
        1 tcttcatatc ttggtgaatt aaggaaatta tccactgaaa tatggatggc atttggtgtt
       61 tgtttaatag cacgatcagt tagttttttc tgagtgataa ctaattgagc atcttttggc
      121 aattgattaa tcgcagtatt tgtaactgta atatcattaa tgcccgcctt tttaaattta
      181 ttacgtaaca tgcttgcacc cattgcacta gaacccatac ccgcatcgca ggcaaaaata
      241 acatgattta tattattgta attgtgtgca tcaacatctt cagtgttgta attatccaat
      301 agcgcttcag gatcatcatc tgatgaagat gtttcactaa cattaccact agcattttct
      361 tctgtattaa catttttatc agaagatact aacttagaag caacgcttga ttttttccct
      421 ttagtatttt ccatttgagc tgtcgcagct tctaaatcct gctttggttc tctagtgaac
      481 ttcataatta aagcagctac aacgaatgat acaagtgcag caaggaagac accgagcaac
      541 atgtgcaaga attcacctct aggtgcattt aaacagtaaa ctataaatga acctggtgac
      601 gcgggacttt taaatccaaa tcctgttgct tgataagttg caacaccagt cattccacct
      661 aaaataacag cgataaataa taaaggacgc attaatacat atgggaaata aatttcatga
      721 ataccaccta agaagtggat aattccagca ccatatgacg ttgcttttgc agtgcctttt
      781 ccaaaaatca tataagcaag taagatacct aaacctggtc cagggttaga ttcaattgtg
      841 tataaaattg attgaccagc ttttgcagct tgatctgcac caagcggtgt gaatacacca
      901 tggttaatcg cattgtttaa aaatacaatt tttgcaggct ctactaaaat acttacaagt
      961 ggaagtaggt gtgcatgtac taatgcttca actgccactg ataaaatatg cataataaat
     1021 ttcataagtg gtgctaaaat tttaaatcct gcaatcgtca tgataaatcc taaaatacca
     1081 gcagaaaagt tattaaataa catttcaaaa ccttgtggcg ttctaggttg aatcaattgg
     1141 tcggtcttct tcattaacca accaacaagt ggacccataa tcattgcacc aagtaacatt
     1201 ggtgtatcag gtaatgcaac gatgaccccc atagttgctg ttgctgcgat gataccacca
     1261 cgtaaatcat aaattaaacg accaccacta aatgcgatca ataatgggat taaataagta
     1321 atcattggtc ctgctaaagt agctaaatct ttgttaggta accatccatt atctataaaa
     1381 atggccgcga tgaaacccca agcgatgaat gcaccaatgt ttggcataat catactactc
     1441 aaaaatgatc cgaatgcttg aacgcgacga ccaattcctt ttttctcttc agtttgcgac
     1501 atatttacac ctctttttta atatatgtga ctatatatag tgtatagaga cgttacttgt
     1561 tactcaatat aaacaaaaat caactttgtc aaaataaatg tgacaaaatt aaataaagtg
     1621 tcatcaatgt gacagtatag atattttgaa aaagtaaaac aaaaaaattg ttttaggatt
     1681 tttaaaattt tattgtgaaa atatttgcaa aacaaaacaa caccgttgta caataatgat
     1741 taatggaaag ggggaaagtt cggcagtaca gttaaagcgc ctgtgcaaat aaatatttgt
     1801 atttgaagat taaaggttaa tatatgagtg gcctttatag agtgcaatat atgtatttgt
     1861 agacgaggag gatagtgatc gaatagatcg gcggatgcta tcccggatgt ggctcattcg
     1921 ttagcttatt aagtaaaaca ttagggtgac ttaatggaca aagttaataa gatcgccaga
     1981 aattgaatat aaaaaatatt aatatggaaa gtacagtgtg agcaatttgt atagttgtaa
     2041 aaataactat gcttaatttg ttatggatga atgcgataat agcatgttcc tatttatatt
     2101 atgaaagcag attgtcaatc taaattatcg gcaataaatc ataatttacg cgtactattc
     2161 caatatggag gaaaatgtcg ttatgtgtgg aattgttggt tatattggct atgataatgc
     2221 caaagaatta ttattaaaag gtttagaaaa attagaatac agaggttatg actctgcagg
     2281 tatcgcggta gtaaatgatg ataatacaac tgtatttaaa gaaaaaggtc gtattgcaga
     2341 attacgtaaa gttgctgata gtagcgattt tgatggacct gttggaatcg gtcacacacg
     2401 ttgggcaaca cacggtgtac cgaatcatga aaactctcat ccacatcaat catcaaatgg
     2461 ccgttttact ctagttcata acggtgttat tgaaaactat gaagagttaa aaggtgaata
     2521 cttacaaggt gtatcattca tttcagaaac agatacagaa gttatcgttc aattagttga
     2581 atacttttca aatcaaggac tttcaactga agaagcattt acaaaagttg tgtcattatt
     2641 acatggttca tatgcattag gtttattaga tgctgaagac aaagacacaa tctatgttgc
     2701 taaaaataaa tcaccattat tattaggtgt tggtgaaggt ttcaatgtta tcgcatcaga
     2761 cgcacttgca atgttacaag tgacaagcga atataaagaa atccatgacc atgaaatcgt
     2821 tattgttaaa aaagatgaag ttattattaa agatgcagat ggaaacgttg tagaacgtga
     2881 ttcatatatt gctgaaattg atgcatcaga tgctgaaaaa ggtgtttatg cacactacat
     2941 gttaaaagaa attcatgaac aaccagcagt aatgcgtcgt attattcaag aatatcaaga
     3001 tgcagaaggt aacttgaaaa ttgatcaaga catcatcaat gatgttaaag aagcagaccg
     3061 catttacgtt attgcagcag gtacaagcta ccatgcaggt ttagtaggta aagaattttt
     3121 agaaaaatgg gctggcgtac caactgaagt acacgttgca tcagagtttg tctacaacat
     3181 gccattatta tctgaaaaac cattgttcgt ttatatttct caatcaggtg aaacagcaga
     3241 tagccgtgca gtattagttg aaactaataa attaggtcat aaatcattaa caatcactaa
     3301 tgttgcaggt tcaactttat cacgtgaagc agaccacaca ttgttattac atgcgggtcc
     3361 tgaaatcgca gttgcatcta caaaagcata tactgcacaa attgcagtat tatcaatctt
     3421 gtctcaaatc gttgcaaaag agcacggtcg tgaagcagat attgatttat tgagagaatt
     3481 agcaaaagta acaacagcaa tagaagcaat tgttgacgat gcaccaatta tggaacaaat
     3541 tgcaacagat ttcttagaaa caacacgcaa tgcattcttt atcggacgta ctattgacta
     3601 taacgtaagt ttagaaggtg cgttaaaact taaagaaatt tcttacattc aagcagaagg
     3661 ttttgctggt ggcgaactta aacatggtac gattgcatta atcgaagatg gcacaccagt
     3721 tgttgcttta gcaactcaag aaaacgttaa tttatcaatt cgtggtaacg ttaaagaagt
     3781 agtagcacgt ggtgcacatc catgtattat ttctatggag ggtcttgaaa aagaaggcga
     3841 cacttatgtc attcctcatg tacatgaatt attgacacca ttagtttcag tagttgcatt
     3901 acaattgatt tcatattatg cagcattaca cagagattta gatgtggata aaccacgtaa
     3961 ccttgctaaa tcagttactg tagaataatt cactttttta gaatcaatca tgtattaaaa
     4021 ttaaagtata tggcaccctt ttagattaat cgactagaag ggtgcttttt taggtcgact
     4081 tagcttttac ttcatcttaa tttggcagaa atgcgtaaaa aggaagtgtt ttatttattt
     4141 aaatagtctg acaattaagg gtgttatgtt aatatgattt tatgagaagt atggagtagc
     4201 aataaagggg tgacctcgca tgttaattca attagatcaa attgggcgaa tgaagcaagg
     4261 aaaaacaatt ttaaaaaata tttcttggca aattgctaaa ggtgataaat ggatattata
     4321 tgggttgaat ggtgctggta agacaacgct attaaatata ttaaatgcat atgagcctgc
     4381 gacaactggc ggtgttaatc tatttggtaa aatgccaggc aaggtagggt attctgcaga
     4441 gactgtacga caacatatag gttttgtatc tcatagttta ctggaaaagt ttcaagaggg
     4501 tgaaagagta atcgatgtgg tgataagcgg tgcttttaaa tcaattggtg tttatcaaga
     4561 tattgatgat gaggtacgta atgaagcaca tcatttactt aaattagttg gaatatctgc
     4621 taaagcgcaa caatatattg gttatttatc tacaggtgaa aaacaacgag tgatgattgc
     4681 acgagcttta atggggcaac ctcaggtttt aattttagat gagccagcag ctggtttaga
     4741 ctttattgct cgtgagtcac tattgaatat acttgactca ttgtcagatt catatccaac
     4801 gcttgcgatg atttatgtga cgcactttat tgaagaaatt actggtaact ttactaaaat
     4861 tttgctatta aaagatgggg aaagtgtaca acaaggatta atagatgata ttttgacatc
     4921 tgaaaacatg tcacgatttt tccagaaaaa tgtagcagtt caaagatgga ataatcgatt
     4981 ttctatggca atgttagagt aaatattttg caaataataa gtaataatga caaaatttaa
     5041 ttaagataaa atggacagtg gagggcaata tggataacgt aaaagcaata tttttggaca
     5101 tggatggaac aattttacat gaaaataatc aagcatcaac gtatacgaaa gatgtcatta
     5161 atcaattgag agagaaagga tataaagtat ttttggcaac aggacgttcg cattctgaaa
     5221 tacatcaact tgtacctcaa gattttgcgg ttaatggcat cattagttca aatggaacaa
     5281 ttggagaagt agatggagaa attatcttca agcatggttt atcattggct caagtgcaac
     5341 aaattactaa tttagctaag cgccaacaaa tttattatga ggtatttcct tttgaaggta
     5401 atagagtttc tttaaaagaa gatgaaacat ggatgcgaga tatgattcgt agtcaagatc
     5461 ctattaatgg cgtaagtcat agtgaatggt cttcaagaca agatgcgctt gctggtaaga
     5521 tagattgggt aactaagttt cctgaaggtg aatattcaaa aatttatcta ttcagttcta
     5581 atttagaaaa aataacagca tttagagatg aattaaagca aaatcatgtg caactacaga
     5641 ttagtgtttc aaattcatca agatttaatg cggaaacaat ggcttatcaa actgataaag
     5701 gtacaggcat taaagaaatg attgcacatt ttggtattca tcaagaagaa acgttagtta
     5761 ttggagatag cgacaatgat agagcaatgt ttgaatttgg tcattataca gttgctatga
     5821 aaaatgcacg ccctgaaatc caagcattaa cttcagatgt aacggcatac acgaatgaag
     5881 aggatggcgc agcaaaatat ttagcaaagc attttttagc tgattaataa aataggtagt
     5941 tatttattat ttaatttaca atagttgatg agtaatgtac aaagagcagt aaagttattt
     6001 tctattagaa aatgtcttac tgctcctttg tatgctagta atccagatac tagtgttact
     6061 tcttcttttt gtctttcttc tccatatatg gaatgaagaa aatcacaaat agggcacaga
     6121 gtatagctac aattgaaccg tatgtcatat tagcatcacc ttttatatgg tttatgcatt
     6181 taagtatact ataacaacta ccgttatttt aagtaggggt gtatttgcgt tagagcccct
     6241 aacaactcca aaatcgcatt tgtgattttg ggataatcaa acatttcatt ccataagtaa
     6301 gatatgtttc actttatatg agttttttac attcacattc tgtagtggtt gtaagaaaat
     6361 tttttctttc tcttaggcag taataggcaa tttacctgta ctagtatgaa cttagtagtt
     6421 aaatcgttca ttaagattaa aaaaacttag gaatctcttc caaatgcaat tcgtcttggt
     6481 aagcattaat ttcatctaat atttttgtta ttgattcaac tttatctttt tctagggagg
     6541 ccggaaatac aaggttttac ttgaaattta aaaccatcag cgaattattt aaatctacga
     6601 tttattcgac cacttgtact ttcttttctt ggcttataca tgactatttt caagttatcc
     6661 gttacaagtt cattatatac accttctatt agctcaaacg attgtgttaa tgaattaaat
     6721 aaggcatcac ttgatttact cattgttatg catgattata aaacgagaat aataaagtag
     6781 tagcacacct atatatagtg gaatcctttg attatctttc gttttaaagt taatgctttc
     6841 tttccagtca aactgtgctt gatgatcagg ttttgtctcg aatcttgttg tatctttagg
     6901 cgacatctat tgataacatt tcataatata aggagtaaat tcatcatgtt taagtatata
     6961 agctctaaat gtataatatg cacaggttaa tccatgtttt tccttcgaat attgccaaag
     7021 tatacgctta tagtaaaatt tttgttcact agtgtctgaa agtaattttt cgaggatagg
     7081 ataatactta tcaataatag actgacgatt tctctttcaa agttttcatt tttcaatgtc
     7141 tattttgaag ggagcatatc agccgaaaag gttggtgctc ttctttatca gagaataaaa
     7201 aatgagaaat taaattttta tatgatgaac aaacgataac aggggaagaa atagctagtt
     7261 agacaagggt atgtcactca actgtttatc gagttgatta agaaatgaaa aaacttatac
     7321 tatgaattac tgtttaaaag tgtgcatgtt ataatattta ttgagcaagt tggatagatg
     7381 gtggctaatc tcttaataaa ggggtgatgc ctatggttat agttgttact cctagaaagg
     7441 actagcatgt ctgattttga aatgctgatg gttgtattaa caatcattgg tttagtattg
     7501 attagtactc aagaccataa aaaataacct tctattcgct ttgaccggca ttttgaaggc
     7561 tatttttaaa taaaatataa ggtcaccgtc tttttaacgg gctcattagg gtaacatgtt
     7621 tccgagtgtt gccctttttg tgtttcaaga gttaaagatt ttcatctttt gcttctactt
     7681 actacaaata tattttaaca catttttctt atgaattgta gttctgaaca taatcagaat
     7741 taataaaacc aactttccat acagcagaaa atacaattaa aagtatagaa tgtaatcacg
     7801 aattatataa aaagtattgg agacctcttc atatatagat aattcactta gttattttag
     7861 aaagaagccc ctaacaacta aagttgaaaa gtaggagcgc ctgcgctaag
//
LOCUS       DAOBBH010000116         7830 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.116, whole genome shotgun sequence.
ACCESSION   DAOBBH010000116 DAOBBH010000000
VERSION     DAOBBH010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7830
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.116"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            169..1089
                     /gene="mvk"
                     /locus_tag="P6L01_001830"
     CDS             169..1089
                     /gene="mvk"
                     /locus_tag="P6L01_001830"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDP3030896.1"
                     /translation="MTRKGYGESTGKIILIGEHAVTFGEPAIAVPFNAGKIKVLIEAL
                     ESGNYSSIKSDVYDGMLYDAPDHLKSLVNRFVELNNITEPLAVTIQTNLPPSRGLGSS
                     AAVAVAFVRASYDFLGKSLTKEELIEKANWAEQIAHGKPSGIDTQTIVSGKPVWFQKG
                     HAETLKTLSLDGYMVVIDTGVKGSTRQAVEDVHKLCEDPQYMSHVKHIGKLVLRASDV
                     IEHHNFEALADIFNECHADLKALTVSHDKIEQLMKIGKENGAIAGKLTGAGRGGSMLL
                     LAKDLPTAKNIVKAVEKAGAAHTWIENLGG"
     gene            1094..2077
                     /gene="mvaD"
                     /locus_tag="P6L01_001831"
     CDS             1094..2077
                     /gene="mvaD"
                     /locus_tag="P6L01_001831"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDP3030897.1"
                     /translation="MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTE
                     TKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAA
                     GLASSASAYAALAAACNQALDLQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYNDE
                     TSYAVPLESNHFEDDLAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLA
                     EAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGY
                     PCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE"
     gene            2090..3166
                     /locus_tag="P6L01_001832"
     CDS             2090..3166
                     /locus_tag="P6L01_001832"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDP3030898.1"
                     /translation="MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEEADQY
                     KGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHF
                     HLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQS
                     LSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENME
                     VLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHRCVEKLIHAFKTNNIKGVQKM
                     VRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVD
                     KEKIYDEWTKHGIKPLKFNIYHGQ"
     gene            3343..3684
                     /locus_tag="P6L01_001833"
     CDS             3343..3684
                     /locus_tag="P6L01_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HDP3030899.1"
                     /translation="MKNTFLICDECQAVNIRTLQKKLEKLDPDAEIVIGCQSYCGPGR
                     RKTFTFVNNRPLAALTEEELIEKVSQQLKKPRDPEEEERLRKRHEERKRRKEEQDRKL
                     KEKLEKRKAQQ"
     gene            complement(3736..5058)
                     /locus_tag="P6L01_001834"
     CDS             complement(3736..5058)
                     /locus_tag="P6L01_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDP3030900.1"
                     /translation="MKTYDLIVIGFGKAGKTLAKYAASTGQHVAVIEQSSKMYGGTCI
                     NIGCIPSKTLVHDGLEGKSFEASYNRKNDVVNALNNKNYHLLADDNNIDVLDFKAQFK
                     SNTEVNLLDQHGDIVDSITAPHIIINTGATSIIPNIKGLDQAKHVFDSTGLLNISYQP
                     KHLVIVGGGYIALEFASMFANLGSKVTVLERGESFMPREDQDVVAHAITDLENKGIAL
                     HTNVETTELSSDDHHTTVRTNVGNFEADAVLLAIGRKPNTALALENTDIELGDRGEIK
                     VNAQLQTSVPHIYAAGDVKGGLQFTYISLDDYRIIKSALYGNQSRTTDNRGSVPYTVF
                     IDPPLSRVGLTSKEAAAQHYDYTEHQLLVSAIPRHKINNDPRGLFKVVINNENNMILG
                     ATLYGKQSEELINIIKLAIDQNIPYTVLRDNIYTHPTMAESFNDLFNF"
     gene            complement(5045..5485)
                     /locus_tag="P6L01_001835"
     CDS             complement(5045..5485)
                     /locus_tag="P6L01_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDP3030901.1"
                     /translation="MNLEFNIAVHVLAFLTKHHSEKFNSSSLAELTCLNPVQLRRVTT
                     QLVDLKMIDTIRGKDGGYLANDQSADVSLATLYKHFVLEKEHHTRLFTGDEGSHCQIA
                     RNIATTMSHYQQDEQNIIINFYNEKTIKDVIEDIQKEDLCHENI"
     gene            5543..5644
                     /locus_tag="P6L01_001836"
     CDS             5543..5644
                     /locus_tag="P6L01_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030902.1"
                     /translation="MCQYLCLKSVELGVVIVMIYCLKVDALTVRLHN"
     gene            6110..7504
                     /locus_tag="P6L01_001837"
     CDS             6110..7504
                     /locus_tag="P6L01_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDP3030903.1"
                     /translation="MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDAL
                     KGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEED
                     IRSLSQQITRYENLTNNLKKHASDNQQAISSNQQIIQTLGQQKHELEEKLRKLREFNQ
                     KSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMG
                     KIEHKAEKETLNKADFAVIKAYAKEHPEDDIPKSIMKYINDNKDSIKRDIGLDITSTL
                     LEQGGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKG
                     VAGVGFGIGMYDDLANDNKTFGEALSHNGMTLAAGSVAAFGVAALVSNPVGWAVVGIL
                     TAGIIGSTVSDYLYQNNYFGIKDKVDWAGHKIDEGIDAVNKTAEKAVDSVGNAIGDAT
                     KSISNHINPMKWSW"
     gene            7505..>7830
                     /locus_tag="P6L01_001838"
     CDS             7505..>7830
                     /locus_tag="P6L01_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDP3030904.1"
                     /translation="MLLCESKVINKNPKYRIIKYESEYLMIDLASNWIVFFFPFINWL
                     IPKTYVKITKNDYEKLNIVKPVKNKSIGWTIFAGIVLLGGTVRRNTYLFDFQLEKLIV
                     WSSCFIG"
BASE COUNT         2770 a         1111 c         1527 g         2422 t
ORIGIN      
        1 agtcaaatat aaattcaaaa taaagattat acataatatg aataatgtga gataaagact
       61 gcaatgaaat ttaacgcgaa tgcgtatgca acttagaata tgatgtcttt tactaataaa
      121 taaaaataat gaatcaagta taggataaat ataaaaggtg atattgacat gacaagaaaa
      181 ggatatgggg aatcgacagg taagattatt ttaataggag aacatgctgt tacatttgga
      241 gagcctgcta ttgcagtacc gtttaacgca ggtaaaatca aagttttaat agaagcctta
      301 gagagcggga actattcgtc tattaaaagc gatgtttacg atggtatgtt atatgatgcg
      361 cctgaccatc ttaagtcttt ggtgaaccgt tttgtagaat taaataatat tacagagccg
      421 ctagcagtaa cgatccaaac gaatttacca ccatcacgtg gattaggatc gagtgcagct
      481 gtcgcggttg cttttgttcg tgcaagttat gattttttag ggaaatcatt aacgaaagaa
      541 gaactcattg aaaaggctaa ttgggcagag caaattgcac atggtaaacc aagtggtatt
      601 gatacgcaaa cgattgtatc aggcaaacca gtttggttcc aaaaaggtca tgctgaaaca
      661 ttgaaaacgt taagtttaga cggctatatg gttgttattg atactggtgt gaaaggttca
      721 acaagacaag cggtagaaga tgttcataaa ctttgtgagg atcctcagta catgtcacat
      781 gtaaaacata tcggtaagtt agttttacgt gcgagtgatg tgattgaaca tcataacttt
      841 gaagccctag cggatatttt taatgaatgt catgcggatt taaaggcgtt gacagttagt
      901 catgataaaa tagaacaatt aatgaaaatt ggtaaagaaa atggtgcgat tgctggaaaa
      961 cttactggtg ctggtcgtgg tggaagtatg ttattgcttg ccaaagattt accaacagcg
     1021 aaaaatattg tgaaagctgt agaaaaagct ggtgcagcac atacatggat tgagaattta
     1081 ggaggttaat gcgttgatta aaagtggcaa agcacgtgca catacgaata ttgcacttat
     1141 aaaatattgg ggtaaaaaag atgaagcact aatcattcca atgaataata gcatatctgt
     1201 tacattagaa aaattttaca ctgaaacgaa agtcactttt aacgaccagt taacacagga
     1261 tcaattttgg ttgaatggtg aaaaggttag tggcaaagaa ttagagaaaa tttcaaaata
     1321 tatggatatt gtcagaaata gagctggcat cgattggtat gctgaaattg aaagcgacaa
     1381 ttttgtacca acagcagcag ggttggcttc atcagcaagc gcatatgcag ctttagcagc
     1441 agcttgtaat caagcactag acttgcagct gtcagataag gatttatcga gattggcgcg
     1501 aattggttcg ggttctgcgt cgcgtagtat ttatggtgga tttgcagaat gggaaaaagg
     1561 gtataatgat gagacgtcat atgccgttcc acttgaatcg aatcattttg aagatgacct
     1621 tgccatgata tttgttgtga ttaatcaaca ttctaaaaag gtacctagtc gatatggtat
     1681 gtcgttgaca cgaaacacat caaggtttta tcaatattgg ttagatcata ttgatgaaga
     1741 tttagctgaa gcaaaagcag cgattcaaga caaagatttt aaacgccttg gtgaagtaat
     1801 tgaagaaaat ggtttacgta tgcatgccac gaatctggga tcaacaccgc cgttcactta
     1861 tcttgtgcaa gaaagttatg atgtcatggc gctcgttcac gaatgccgag aagcgggata
     1921 tccgtgttat tttacgatgg atgcgggtcc taatgtgaaa atacttgtag aaaagaaaaa
     1981 caagcaacag attatagata aattattaac acagtttgat aataaccaaa ttattgatag
     2041 tgacattatt gccacaggaa ttgaaataat tgagtaagga agagataaaa tgattcaggt
     2101 caaagcaccc ggaaaacttt atattgctgg agaatatgct gtaacagaac caggatataa
     2161 atctgtactt attgcgttag atcgttttgt aactgctact attgaagaag cagaccaata
     2221 taaaggtacc attcattcaa aagcattaca tcataaccca gttacattta gtagagatga
     2281 agatagtatt gtcatttcag atccacatgc agcaaaacaa ttaaattatg tggtcacagc
     2341 tattgaaata tttgaacaat acgcgaaaag ttgcgatata gcgatgaagc attttcatct
     2401 gactattgat agtaatttag atgattcaaa tggtcataaa tatggattag gttcaagtgc
     2461 agcagtactt gtgtcagtta taaaagtatt aaatgaattt tatgatatga agttatctaa
     2521 tttatacatt tataaactag cagtgattgc aaatatgaag ttacaaagtt taagttcatg
     2581 cggagatatt gctgtgagtg tatatagtgg atggttagcg tatagtactt ttgatcatga
     2641 atgggttaag catcaaattg aagatactac ggttgaagaa gttttaatca aaaactggcc
     2701 tggattgcac atcgaaccat tacaagcacc tgaaaatatg gaagtactta tcggttggac
     2761 tggctcaccg gcgtcatcac cacactttgt tagcgaagtg aaacgtttga aatcagatcc
     2821 ttcattttac ggtgacttct tagaagattc acatcgttgt gttgaaaagc ttattcatgc
     2881 ttttaaaaca aataacatta aaggtgtgca aaagatggtg cgtcagaatc gtacaattat
     2941 tcaacgtatg gataaagaag ctacagttga tatagaaact gaaaagctaa aatatttgtg
     3001 tgatattgct gaaaagtatc acggtgcatc taaaacatca ggcgctggtg gtggagactg
     3061 tggtattaca attatcaata aagatgtaga taaagaaaaa atttatgatg aatggacaaa
     3121 acatggtatt aaaccattaa aatttaatat ttatcatggg caataaatga tttgataggg
     3181 gagcaatcat ctaatttaat tagaagataa atgctccatt ttttattgcc agccgtgtta
     3241 cgtatcttga aaaaaatatt aaaaagagta aaatagatag agttaacgaa aaaattaatg
     3301 aaatcgacga tatagatatg ataaagaaag gtgggtagca atatgaaaaa tacattcctt
     3361 atttgtgatg aatgtcaggc agtcaatata agaacgttac aaaagaagtt ggaaaaatta
     3421 gatcccgatg ctgaaatcgt gataggttgt caatcttatt gtggacctgg acgccgaaaa
     3481 acattcactt ttgttaataa ccgcccactg gctgcgctta ctgaagaaga attaatcgaa
     3541 aaagtttctc aacaattaaa gaaaccacgt gatcctgaag aagaagagcg tttaagaaaa
     3601 cgacatgaag aacgtaaacg tcgtaaagaa gaacaagata gaaagcttaa agaaaaatta
     3661 gaaaagcgaa aagcacaaca ataaaacctg atggcagcat cattcaatgc gtgccaccag
     3721 gtttttatgt tttgtctaga aattaaataa atcattaaat gattcggcca tcgtaggatg
     3781 tgtataaata ttatctcgta atacggtata tggaatgttt tgatcaatcg caagtttaat
     3841 tatattaatt aattcttcag attgcttacc atataatgta gcacctaaaa tcatattatt
     3901 ttcattatta atgactactt taaataaacc tcttggatca ttgttaattt tgtgacgagg
     3961 tatagcactt actaaaagtt gatgttcagt gtaatcataa tgttgagcgg cagcttcttt
     4021 actagttaat ccaacacgtg ataatggtgg atctataaat actgtataag gcacgctacc
     4081 tctattgtca gtcgtacgtg actgattacc atataacgct gatttgataa ttcgataatc
     4141 atctaaagat atatacgtaa attgaagtcc gcctttaaca tcacctgcag cataaatatg
     4201 tggcacagat gtttgaagtt gagcattgac tttaatttcg cctctatcac ctaattcgat
     4261 atcagtattt tctaaagcta aggctgtatt cggtttgcgc ccgatagcca aaagtactgc
     4321 atcagcctca aagttgccaa cattagtacg gactgttgta tgatgatcgt cagatgacaa
     4381 ttcagtcgtt tcaacatttg tatgcaatgc aatgccttta ttttctaagt cagtaatagc
     4441 atgtgcaacg acatcttgat cttcgcgtgg cataaatgat tcaccacgtt ctaatactgt
     4501 taccttacta cctaaattcg caaacattga agcaaattct aaggcaatat aaccgccacc
     4561 tacaataacg agatgcttag gttgatagct aatgtttaat aaacctgttg aatcgaagac
     4621 atgtttagct tgatcaaggc cttttatgtt aggaatgata gaggtagcac cggtattaat
     4681 aatgatatga ggtgcagtaa tactatcgac gatatcgcca tgctgatcta ataaattcac
     4741 ttcagtatta gatttaaact gcgctttaaa atccaataca tcaatgttgt tatcgtctgc
     4801 taataagtgg taatttttat tgtttagcgc attgacaaca tcgtttttac ggttataact
     4861 tgcttcaaaa gatttgcctt ctaatccatc atgtacaagt gtcttcgaag gtatacatcc
     4921 tatatttata caagtgccgc catacatttt cgaagattgt tcgataactg cgacgtgttg
     4981 acctgttgat gcagcatatt tcgctaaagt tttaccagct ttcccaaatc ctattacaat
     5041 taaatcatat gttttcatga cataaatcct ccttttgaat gtcttcaatg acatctttga
     5101 ttgttttttc attataaaaa ttaataatga tattctgttc gtcttgctga taatgtgaca
     5161 tggtagttgc aatattacga gcaatttgac agtgactgcc ttcgtcgcca gtaaatagac
     5221 gtgtgtggtg ttctttctct aagacaaaat gtttatataa tgttgctaga gagacatcag
     5281 cactttgatc atttgctaaa taaccgccat ctttacctcg tattgtgtca atcattttta
     5341 aatcgacaag ttgagtcgtc acgcgtcgta attgaacagg atttaaacaa gttaattctg
     5401 ctaatgaact actattgaat ttttctgaat gatgcttagt taaaaaagct aatacatgca
     5461 cggcaatgtt aaattctaaa ttcaatgttt ctcacctcat aattgtaact gtattagtta
     5521 caattaaaaa tcattttgct caatgtgtca atacttatgt ttgaaaagtg ttgaattagg
     5581 tgtggttatt gtgatgattt attgtttgaa agttgatgca ttaacggtga gattgcacaa
     5641 ttgaaatttt aattatgtat gaactgatag atatgaaatg aatattgaaa atgagagcaa
     5701 taaaattttg aatgatgatg ttgtgtgatt gtataaactt attcgtattt tagatacgtt
     5761 ttgatatttc gcatgatgag gaaatacttt taacaactga tgacatattt taatagtgaa
     5821 tatattttga gatattaata tatttaatgt taaggatgtt gtcgcaataa aattaaaata
     5881 ttgaaagata atttttattg attaattgcg aatatacaaa gacaccatta tatccatatg
     5941 ctaaaataag aaaaaaggcg acatgttatc tcaatttacc tgcatctaga ctcctttaca
     6001 taagaaataa tcatcgccga gttttaaaat tttacaactt tgtagatgta aaaagcattt
     6061 gattttaaat agtttgaaaa tcatatattt ttgaaaaagg agcatgccga tgagtattga
     6121 catgtattta gacagatctc gaaaccaagc ttcaagtgtg gggaatttga gtcaaacaat
     6181 gaattcaaat tatgatgcgt tggaaaaagc aattactcaa tttatcaatg atgatgcgct
     6241 taaagggaaa gcgtatacgt cagctaagca attttttagt acggtgttaa ttccattatc
     6301 aacaagtatg aaaacattga gtgatttaac gaagcaagct tgcgataatt ttgtgtcacg
     6361 ttatacgagt gaggttgata gcatatcttt aaaagaatca gagcttgaag aagatatcag
     6421 atcattaagt caacaaatta cgcgatatga aaatttaact aacaatttga aaaagcatgc
     6481 ttccgataat cagcaagcca tttcatcgaa ccaacaaata atacaaacat taggtcaaca
     6541 aaaacatgaa ttagaagaga aactacgtaa attacgtgag tttaatcaaa aatcaccaga
     6601 aatatttaaa gaagttgaag aatttcaaaa aattgtccaa caaggactta cccaagcgca
     6661 gaatttttgg aacttttcaa caaatcaatt taatatacct tcaggtaaag aacttgattg
     6721 ggctaaagca agtcatgaaa aatatttgaa agttgctatg gggaaaattg aacataaagc
     6781 agagaaagaa actttaaata aagcagactt tgctgttata aaggcatatg ccaaagaaca
     6841 tccagaagac gatatcccga aaagtataat gaaatatata aatgacaata aagatagtat
     6901 taaacgagat ataggattag atattacgtc aacactttta gagcaaggcg gtataaatgc
     6961 aagtaaattc ggtgtattta tcaatacagc aggtggagtg aaaggcccag ccggtccaaa
     7021 ttcatttgtg gaagtcaaac gtacatcagg taatgtgttt atagaaaatg gtagtaaatt
     7081 tgcaaaaggc ggaaaatatc tagggaaagg tgttgcaggt gtaggatttg gtataggtat
     7141 gtatgatgat cttgcaaatg ataataaaac atttggagag gcattgtcgc ataatggtat
     7201 gacgcttgca gctggatctg ttgcagcatt tggtgtagcg gctttagtaa gtaatccagt
     7261 cggttgggct gtggtaggta ttttaacagc aggtattatt ggatctactg tttctgatta
     7321 tctttatcaa aataattatt ttggcataaa agataaagtg gactgggcag gacataaaat
     7381 agacgagggt attgatgctg taaacaaaac tgcagaaaaa gctgtagata gtgttggtaa
     7441 tgccattggt gatgccacaa aaagtattag taatcatatt aatcctatga aatggagttg
     7501 gtaaatattg ctttgcgaat ctaaagttat taataaaaat cctaaatatc gaataatcaa
     7561 atacgaaagt gaatacttaa tgattgattt agcaagtaat tggattgttt tcttctttcc
     7621 atttattaac tggctcatac cgaaaacata tgtcaaaatc actaagaatg attatgaaaa
     7681 attaaatatt gtcaaaccag ttaaaaataa atcgatagga tggactatat tcgcgggtat
     7741 tgtgttactt ggtggtactg taagaagaaa tacttattta tttgattttc aattagaaaa
     7801 actaattgtt tggagcagct gttttattgg
//
LOCUS       DAOBBH010000117         7758 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.117, whole genome shotgun sequence.
ACCESSION   DAOBBH010000117 DAOBBH010000000
VERSION     DAOBBH010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7758
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.117"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            68..511
                     /locus_tag="P6L01_001839"
     CDS             68..511
                     /locus_tag="P6L01_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDP3030905.1"
                     /translation="MNIYDEYRSYLIEELDDCLTIQKNNDTAYYDVLEAINDLSNDSL
                     CVLNHLYINEGQEETFEQKFLQRNKHLKNVDGFKALRFLRPRTAGRHYIIITLWENRQ
                     AFYHWQNSAEYKHTHKHRGTSKGADVKIINRELSYNIRIELADMV"
     gene            705..2303
                     /locus_tag="P6L01_001840"
     CDS             705..2303
                     /locus_tag="P6L01_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDP3030906.1"
                     /translation="MQKSALFAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTY
                     QQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRT
                     KDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASN
                     KLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWAT
                     AAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMA
                     KLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDT
                     PQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKAQ
                     TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA
                     KPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS
                     GKIRRVELRDSEIEKWQQQKDSNQ"
     gene            complement(2363..2692)
                     /locus_tag="P6L01_001841"
     CDS             complement(2363..2692)
                     /locus_tag="P6L01_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030907.1"
                     /translation="MAYSILNLYSILIQYKCKINHKKEIQYLHPRKTLQLPLILKIIL
                     NLALIGIVIIVLTHTELQLNAGDSNVFLLNWDIRGLTSYIFSFLILVALVSKLILIFI
                     TRRQRTS"
     gene            2988..4484
                     /gene="mqo"
                     /locus_tag="P6L01_001842"
     CDS             2988..4484
                     /gene="mqo"
                     /locus_tag="P6L01_001842"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDP3030908.1"
                     /translation="MAKSNSKDIVLIGAGVLSTTFGSMLKEIEPDWNIHVYERLDRPA
                     IESSNERNNAGTGHAALCELNYTVLQPDGSIDIEKAKVINEEFEISKQFWGHLVKSGS
                     IENPREFINPLPHISYVRGKNNVKFLKDRYEAMKAFPMFDNIEYTEDIEVMKKWIPLM
                     MKGREDNPGIMAASKIDEGTDVNFGELTRKMAKSIEAHPNATVQFNHEVVDFEQLSNG
                     QWEVTVKNRLTGEKFKQVTDYVFIGAGGGAIPLLQKTGIPESKHLGGFPISGQFLACT
                     NPQVIEQHDAKVYGKEPPGTPPMTVPHLDTRYIDGQRTLLFGPFANVGPKFLKNGSNL
                     DLFKSVKTYNITTLLAAAVKNLPLIKYSFDQVIMTKEGCMNHLRTFYPEARNEDWQLY
                     TAGKRVQVIKDTPEHGKGFIQFGTEVVNSQDHTVIALLGESPGASTSVSVALEVLERN
                     FPEYKTEWAPKIKKMIPSYGESLIEDEKLMRKIRKQTSKDLELGYYEN"
     gene            complement(4678..5568)
                     /locus_tag="P6L01_001843"
     CDS             complement(4678..5568)
                     /locus_tag="P6L01_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HDP3030909.1"
                     /translation="MNKEQLEKMKNGKGFIAALDQSGGSTPKALKEYGVNEDQYSNED
                     EMFQLVHDMRTRVVTSPSFSPDKILGAILFEQTMDREVEGKYTADYLADKGVVPFLKV
                     DKGLAEEQNGVQLMKPIDNLDSLLDRANERHIFGTKMRSNILELNEQGIKDVVEQQFE
                     VAKQIIAKGLVPIIEPEVNINAKDKAEIEKVLKAELKKGLDSLNADQLVMLKLTIPTE
                     PNLYKELAEHPNVVRVVVLSGGYSREKANELLKDNDELIASFSRALASDLRADQSKEE
                     FDKALGDAVESIYDASVNKN"
     gene            complement(5691..6107)
                     /locus_tag="P6L01_001844"
     CDS             complement(5691..6107)
                     /locus_tag="P6L01_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030910.1"
                     /translation="MRLKVLFHFIAAIFISFMLLWMTMLFDLISNQSHLKALLLNLDF
                     LIPSDNTPYILEIICHLLIGSVIYFVFVLLFHTSKRLYYLCYIPLFFLFIALYPFLVF
                     IAQRPIFQFSVTELIGWIITHIFFMSLMALVIPRIK"
     gene            6365..7702
                     /locus_tag="P6L01_001845"
     CDS             6365..7702
                     /locus_tag="P6L01_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDP3030911.1"
                     /translation="MSKAHQLIQEDEHYFAKSGRIKYYPLVIDHGYGATLVDIEGKTY
                     IDLLSSASSQNVGHAPREVTEAIKAQVDKFIHYTPAYMYHEPLVRLAKKLCELAPGDF
                     EKRVTFGLTGSDANDGIIKFARAYTGRPYIISFTNAYHGSTFGSLSMSAISLNMRKHY
                     GPLLNGFYHIPFPDKYRGMYEQPQANSVEEYLAPLKEMFAKYVPADEVACIVIETIQG
                     DGGLLEPVPGYFEALEKICREHGILIAVDDIQQGFGRTGTWSSVSHFNFTPDLITFGK
                     SLAGGMPMSAIVGRKEIMNCLEAPAHLFTTGANPVSCEAALATIQMIEDQSLLQASAE
                     KGEYVRKRMDQWVSKYNSVGDVRGKGLSIGIDIVSDKKLKTRDASAALKICNYCFEHG
                     VVIIAVAGNVLRFQPPLVITYEQLDTALNTIEDALTALEAGNLDQYDISGQGW"
BASE COUNT         2724 a         1212 c         1427 g         2395 t
ORIGIN      
        1 gggaatgaat tagtcataga tataaagtaa tatgaccaaa aatataaatg aaatgaaggc
       61 atttattatg aacatttatg atgaatatag aagttattta atagaagaac tggatgattg
      121 tctcacaata caaaaaaata atgacactgc atattacgat gtgctagaag cgataaatga
      181 tttatcaaat gattcattat gtgtgttaaa tcatttatat attaacgaag gacaagaaga
      241 aacattcgag caaaaatttt tgcaacgtaa taagcattta aaaaacgttg atgggtttaa
      301 agcattaagg tttttaagac ctagaacagc tggtagacac tatatcatta ttacgctgtg
      361 ggaaaatagg caagcatttt atcattggca aaattcagct gagtacaagc atacacacaa
      421 gcatcgtggc acttcgaaag gtgcagatgt aaaaataatt aatcgtgaat tatcatacaa
      481 tattcgtatt gaattagctg atatggtcta gcccgagata tcaaccctca atcatttgaa
      541 agtccttgaa tgctacgtca tggcaaagtg tagtgccgag gactttggta tttaacccta
      601 tttaattgaa aattctgtaa agataggcgt atgatatata taattcattt ataaaattac
      661 aatgttcatc acagggaaag tggatgtatg aaaggaagtt agttatgcaa aagtcagctc
      721 tattcgcgcc tgaaaaatat aacattattt cggaaatcga aaaatatagc cacataccag
      781 ataaaaaggc aattttatat cataatactg aaggcgaaga tatatcagtc acttaccaac
      841 agctcattga acagtctaat aaagtaggta atgttttagc atcgcacggt ttatctaaag
      901 gcgataaagt actcattatg atgccacgat ctattgcgac gtatgaatta tatattgctg
      961 ctttaaaatt aggtgttgca atcattccat gttctgaaat gctaagaact aaagatttac
     1021 aataccgtat cacgcacggt gaaataaatg cagttattgc actagagcca ttcacagttg
     1081 aatttgaaaa aattaaagaa tatgatgcgt taactaaatt tgttattgcc ggtcaaaaag
     1141 atggatggat ttcacttgaa gctgaaaaag aaaacgcaag taacaaacta gaaatggcag
     1201 atacgacgcg tgatgatatg gcgattctgt catatacatc tggtacaact ggcaatccta
     1261 aagccgttac acattgtcat ggatggggat ttgcacattt acaaatggca ccaaaacatt
     1321 ggttatgtat taatgaagat gacttagttt gggcaacagc agcaccaggt tggcaaaaat
     1381 gggtatggag tcctttctta tctgtattag gttcaggtgc aactgctttt gtatttaatg
     1441 gaaggttcag tcctgaaaca tacttagaat tgcttcaaaa atatcaaatt aatgttttat
     1501 gttgtacacc gacagaatat cgtatgatgg ctaaattaca gaacttaaat gattatgact
     1561 taacgcattt acatagtgcc gtttctgcag gtgagccact aaatcgagaa gtagttgaac
     1621 aattcaagaa atactttaat ttaactgtaa gagatggata tggccagact gaaagtacct
     1681 tgcttatagg ctttttaaaa gatacaccac agaggattgg atcaatgggt aaaggcattc
     1741 caggtagttc ggtcactgta gtagatgatg aggggaatag cgtacctgct aatacgaaag
     1801 ggaatattgc agtaccactt gatttgccag cacttttcaa agggtattat aaagagccag
     1861 aacgtacggc gaaagcgcaa acaggtaaat actacatcac tggtgactta gctcatgtag
     1921 atgatgatgg ttatttctgg tttgaaggac gtcgagatga cattattatt agttcaggct
     1981 atacgattgg gccttttgaa gttgaagatg cgttaacgaa tcatccagcc gttaaagaat
     2041 gtgctgtagt agcaaaacct catgatatac gaggaaatat tgttaaagca ttcgttattt
     2101 tacaggacca tactgcgggt gatgatacgt tagttaaaga actacaacaa tttgttaaaa
     2161 atgaagttgc accatataaa tatccgagag agattgaatt cgttgacgac ttaccaaaaa
     2221 caaactcagg taaaattcgt cgtgttgaac tacgagattc tgaaatagaa aaatggcaac
     2281 aacaaaaaga tagcaatcaa taaacatagc aaagcagtcc aatgcgtaag gtatgatgat
     2341 acgcactgga ctgcttgttt atttatgatg ttctttgtcg tctagttata aatattaaaa
     2401 ttagtttaga aacaagcgca accagtatta agaatgagaa aatataagat gttaaacctc
     2461 gaatatccca gtttaataaa aacacattac tatcaccggc attaagttga agctcagtat
     2521 gtgttagaac gataattaca atacctataa gtgcaagatt caaaataatt ttcaatatta
     2581 aaggtaattg taatgttttt cttggatgca gatattgaat ctccttttta tgattgattt
     2641 tacacttata ttgtatcaaa atagagtata ggtttaaaat gctataagcc aaatgtagat
     2701 taaaacaatc gttagctatt tgttgtcaaa taattatgat tgatttgata tttatcttct
     2761 atgaataaaa gatttaatat attgcaaagt taatgaataa ttataattta catttaccac
     2821 tgcttgcttt aacatataca tgtaagcact aactatagta ttttgttaca gcgtatcaat
     2881 taaaacagga agttatctat ggcgttgata ttttaaagaa atatgtcagt tttatgtaac
     2941 tttcatattg atgatacaca tttacaattt ttggagaggt gaaaccaatg gctaagtcta
     3001 atagtaaaga catcgtttta attggagccg gtgtacttag cacaacattt ggttcaatgt
     3061 taaaagaaat tgagccagac tggaatatcc acgtttacga acgcttggat cgtcctgcaa
     3121 tcgaaagttc aaacgaaaga aataatgctg gtacgggtca tgcagcatta tgtgagttga
     3181 actacacagt tttacaacct gatggttcta tcgacatcga aaaagcgaaa gtgattaacg
     3241 aagagtttga gatttcaaaa caattctggg gtcacttagt gaaaagcggt agcatcgaga
     3301 acccaagaga atttatcaat ccattaccac acatcagtta tgttagaggt aaaaacaatg
     3361 ttaaattctt aaaagatcgt tacgaagcga tgaaagcttt ccctatgttc gataatatcg
     3421 aatatactga agacatcgaa gtaatgaaaa aatggattcc attgatgatg aaaggccgtg
     3481 aagataaccc tggtatcatg gcggcaagta aaattgacga aggtacagat gtaaacttcg
     3541 gtgaattaac acgtaaaatg gctaaaagca ttgaagcaca tccaaatgct acagtgcaat
     3601 ttaaccatga agttgttgat tttgaacaat tatcaaatgg tcaatgggaa gttactgtta
     3661 aaaatcgcct aactggtgag aaattcaaac aagtaactga ctatgtattc atcggtgctg
     3721 gcggtggagc gattccatta ttacaaaaaa caggtatccc tgaaagtaaa catttgggtg
     3781 gattccctat cagtggtcaa ttcttagctt gtacaaaccc acaagttatt gaacaacacg
     3841 atgccaaagt ttatggtaaa gagccacctg gtacaccacc aatgactgta cctcatttag
     3901 atacgcgtta cattgatggt caaagaacat tattatttgg accatttgct aatgttggac
     3961 ctaaattctt gaaaaatggt tctaacttag atttattcaa gtctgttaaa acatacaaca
     4021 ttacaacttt attagcagca gcagttaaaa acttaccttt aattaaatac tcatttgatc
     4081 aagtgattat gacaaaagaa ggttgtatga accacttacg tactttctat ccagaagcac
     4141 gtaatgaaga ttggcaatta tacactgctg gtaaacgtgt acaagttatc aaagatacac
     4201 ctgaacacgg taaaggattc atccaattcg gtacagaagt ggttaactca caagaccaca
     4261 ctgtaattgc attattaggt gaatcaccag gggcttcaac ttcagtttca gttgcgttag
     4321 aagtattaga acgtaacttc cctgaataca aaactgaatg ggcacctaaa atcaagaaaa
     4381 tgattccatc atacggtgaa tcattaattg aagacgaaaa attaatgaga aaaatccgta
     4441 aacaaacttc aaaagactta gaattaggtt actacgaaaa ctaaattcta atagctatat
     4501 ttaaaatcgg acaactgaga aatcttaaag atttttcagc tgtccgattt ttttacgtaa
     4561 tctaatacat acggcaaatt caactttaat caaaaaagac tcatacacaa tgcccttaaa
     4621 gcacatgtat gagtcctttt tagtagttta tatcaaaaaa tacgtttaat gtataaatta
     4681 gtttttgttt acagatgcgt cgtagattga ttctacagca tcacctaaag ctttatcgaa
     4741 ttcttcttta gattgatcag ctcttaaatc actagctaat gcacgtgaga aacttgcgat
     4801 aagttcatcg ttatctttaa gtaattcatt tgctttttct ctgctgtaac cacctgataa
     4861 tacaacgaca cgaacaacat taggatgttc agctaactct ttgtataagt ttggttcagt
     4921 aggaattgtt aatttcaaca ttactaattg atcagcattt aagctatcta aacctttttt
     4981 aagttcagct tttaatactt tttcaatttc agctttgtct tttgcattaa tattaacttc
     5041 tggttcgata attggcacta aacctttagc aataatttgt ttagcaactt caaattgttg
     5101 ttcaacaacg tctttgatac cttgctcatt taattctaaa atgttagaac gcatttttgt
     5161 accaaaaatg tgacgttcgt ttgcacggtc taataaactg tctaagttgt cgattggttt
     5221 cattaattga acaccatttt gctcttcagc aagacctttg tctactttta agaacggaac
     5281 aacaccttta tcagctaagt aatctgcagt gtatttgcct tctacttcgc gatccattgt
     5341 ttgttcgaaa agaatagcac ccaaaatttt atctggtgag aatgaaggtg aagttaccac
     5401 acgtgtacgc atatcgtgaa caagttggaa catttcgtct tcattgctat attgatcttc
     5461 gtttacacca tattctttaa gtgcttttgg tgtactacca ccactttggt ctaatgcggc
     5521 aataaagcct tttccatttt tcattttttc taattgctct ttattcatac tttccactcc
     5581 ttaacttttc aatacacctc cagtatgata aaaatgagaa catttctcaa gtcataaacc
     5641 ttgaaaagtg tataaaatgt gaaaaataat tgtcagttta attaaaaata ttattttatt
     5701 ctaggtatga ctaacgccat taatgacata aagaaaatat gtgtaataat ccaaccgatt
     5761 aattctgtca cactaaattg aaaaattgga cgttgcgcaa taaatactaa aaagggatac
     5821 aatgctataa ataagaaaaa taaagggata taacatagat agtaaagcct tttagaagta
     5881 tgaaacagca acacaaatac aaaataaatg acgctaccaa ttagtaagtg acatattatt
     5941 tctagaatat atggtgtatt gtcagaagga attagaaaat ctaaattcaa taataaagct
     6001 tttaaatgtg attgattgct tattaagtca aacaacatgg tcatccatag caacatgaat
     6061 gatatgaaaa tagcagcaat gaaatggaac aatactttta aacgcattcg tatcacctca
     6121 gagtcaaatg gaatatagta aacaattccc taatcgaaca aaattatgcg cataaacaaa
     6181 gtagattgat ataaaattct taattatcag aatatattta caaatctgaa ttttattagt
     6241 atattggata gtattcatag aggcatgacg gtatttgagc aagattttaa atcggatttt
     6301 ataatcgatt taagagaggc cactttgctt gcacattaat actgtcaatg ggaggggaat
     6361 gtatatgagt aaagcacatc aattaattca agaggatgaa cattattttg cgaaatcagg
     6421 acgtattaaa tattatccgt tagtgattga tcatggatat ggagcaacat tggttgatat
     6481 tgaggggaag acatatatcg atttgttatc gagtgcaagt tctcaaaacg taggtcatgc
     6541 acctagagaa gtaacagaag cgataaaggc acaagttgat aaatttattc attatacacc
     6601 agcatatatg tatcatgaac cgttggtacg tttagctaag aagctttgtg agcttgcacc
     6661 tggagatttt gaaaaaagag tgaccttcgg attaaccgga tcagacgcaa atgatggcat
     6721 cattaaattt gccagagcat atacagggcg tccttatatc attagtttca ctaatgcata
     6781 tcatggttca acttttggct cattgtctat gtcagctatt agtttaaata tgcgcaaaca
     6841 ttatggtccg ttattgaatg gtttttatca tattccgttt ccagataaat atcgtggtat
     6901 gtacgagcag ccacaagcta attcagtaga agaatattta gcacccttaa aagaaatgtt
     6961 tgcgaaatat gtacctgctg acgaagtagc atgtattgtt attgaaacga tacaaggcga
     7021 tggtggactt ttagaaccag ttccagggta ttttgaagcg ttagaaaaga tttgtcgtga
     7081 acatggtatt ttaatcgctg tcgatgatat tcaacaaggt tttgggcgaa caggtacatg
     7141 gagttcagtc tcgcatttta attttacgcc tgatttaatc actttcggaa aatccttagc
     7201 aggtggtatg cccatgtcag caattgttgg acgcaaagag attatgaatt gtttagaagc
     7261 accagcacat ttatttacaa caggtgctaa tccagttagt tgtgaagctg cattagccac
     7321 aattcaaatg attgaagatc agtcgcttct tcaggctagt gcggaaaaag gggaatatgt
     7381 taggaaacga atggatcaat gggtatctaa atacaatagt gtaggcgatg ttagaggtaa
     7441 aggtctgagc attggtattg atattgtttc cgacaaaaaa ctcaaaacac gtgatgccag
     7501 tgcggcactt aaaatttgta attactgctt tgagcatggc gtagttatta tagctgtagc
     7561 aggaaatgtg ttgcgattcc aaccgccatt ggtgataaca tatgagcaat tagacacggc
     7621 gttaaacact atagaagatg cactgactgc tttggaagca ggtaacttag atcaatatga
     7681 catatctgga caaggttggt aatagcgatt atcttaatat aaaataaaaa atcatttcca
     7741 catctggatg ttaatcag
//
LOCUS       DAOBBH010000118         7723 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.118, whole genome shotgun sequence.
ACCESSION   DAOBBH010000118 DAOBBH010000000
VERSION     DAOBBH010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7723
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.118"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..211
                     /locus_tag="P6L01_001846"
     CDS             <1..211
                     /locus_tag="P6L01_001846"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="catalyzes the formation of D-glyceraldehyde
                     3-phosphate and acetaldehyde from
                     2-deoxy-D-ribose-5-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-deoxyribose-5-phosphate aldolase"
                     /protein_id="HDP3030912.1"
                     /translation="TSTGFAGGGATAEDVKLMKDTVGADVEVKASGGVRNLEDFNKMV
                     EAGATRIGASAGVQIMQGLEADSDY"
     gene            490..1791
                     /locus_tag="P6L01_001847"
     CDS             490..1791
                     /locus_tag="P6L01_001847"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDP3030913.1"
                     /translation="MRMIDIIEKKRDGHTLTTEEINFFIDGYVKGDIPDYQASSLAMA
                     IYFQDMNDDERAALTMAMVNSGDMIDLSDIKGVKVDKHSTGGVGDTTTLVLAPLVAAV
                     DVPVAKMSGRGLGHTGGTIDKLEAIDGFHVEIDEATFVKLVNENKVAVVGQSGNLTPA
                     DKKLYALRDVTGTVNSIPLIASSIMSKKIAAGADAIVLDVKTGSGAFMKTLEDAEALA
                     HAMVRIGNNVGRNTMAIISDMNQPLGRAIGNALELQEAIDTLKGQGPKDLTELVLTLG
                     SQMVVLANKAETLEEARALLIEAINSGAALEKFKTFIKNQGGDETVIDHPERLPQAQY
                     QIEYKAKKSGYVTELVSNDIGVASMMLGAGRLTKEDDIDLAVGIVLNKKIGDKVEEGE
                     SLLTIHSNRQDVDDVVKKLDSSITIADHVVSPTLIHKIITE"
     gene            1805..2155
                     /locus_tag="P6L01_001848"
     CDS             1805..2155
                     /locus_tag="P6L01_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030914.1"
                     /translation="MNILRYLLGIGFSVIGVLHFTRERQFRNIIPKCLPLRKTAVLVT
                     GIFEILFGLALLIKRPSQCLKNTINLFLLAVFPANIYMAVKKLPLGDKQLPTWQLYLR
                     LPMQFVLMGLIKKL"
     gene            complement(2307..2777)
                     /locus_tag="P6L01_001849"
     CDS             complement(2307..2777)
                     /locus_tag="P6L01_001849"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDP3030915.1"
                     /translation="MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQ
                     PNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFINHDNYDDVLNIV
                     EATLNDVLNATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK"
     gene            3088..4272
                     /locus_tag="P6L01_001850"
     CDS             3088..4272
                     /locus_tag="P6L01_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDP3030916.1"
                     /translation="MSEKQQILDYIETNKYSYIEISHRIHERPELGNEEIFASRTLID
                     RLKEHDFEIETEIAGHATGFIATYDSGLDGPAIGFLAEYDALPGLGHACGHNIIGTAS
                     VLGAIGLKQVIDQIGGKVVVLGCPAEEGGENGSAKASYVKAGVIDQIDIALMIHPGNE
                     TYKTIDTLAVDVLDVKFYGKSAHASENADEALNALDAMISYFNGVAQLRQHIKKDQRV
                     HGVILDGGKAANIIPDYTHARFYTRAMTRKELDILTEKVNQIARGAAIQTGCDYEFGP
                     IQNGVNEFIKTPKLDDLFAKYAEEVGEAVIDDDFGYGSTDTGNVSHVVPTIHPHIKIG
                     SRNLVGHTHRFREAAASVHGDEALIKGAKIMALMGLELITNQDVYQDIIEEHAHLKGN
                     GK"
     gene            4272..5465
                     /locus_tag="P6L01_001851"
     CDS             4272..5465
                     /locus_tag="P6L01_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDP3030917.1"
                     /translation="MTNNGNEPNLTLSDLYDKDVVYTSRPSYISNPWLKPDEHQSNFL
                     TGRELLIANQLPVIVHEASATDKLHQLFQVIGKEVPNSIFTFNNQQSYENLIKQLAHK
                     ENKKIYFQYIHDETILNQQYYALDKTLFVALNNKARIPEWTNGKFLPKRKVVKIEQFE
                     NEIKNWEFPLVIKPGDDLPTAGGYGVMICYHDADLQKAITRIKEATAETNSLIIEQKI
                     EEKANYCVQFAYSESLGIQYLGAATQLTDKYGFYNGNENTTNVPEHVIEAGRQIMENG
                     VNQGFFGVAGFDLLVDEDDNVYAIDLNFRQNGSTSMLLLANELNSGYQKFYSYHSKGD
                     NTHFFNTILKYVKEGSLYPLSYYDGDWYGEDKVKSRFGCIWHGDSKETVLENERAFLA
                     ELEHY"
     gene            5864..6535
                     /locus_tag="P6L01_001852"
     CDS             5864..6535
                     /locus_tag="P6L01_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HDP3030918.1"
                     /translation="MSYKAYPFFRDILINECIYFASKNKKLVRLNYKSEAYVGVWTEE
                     SVAVSFLTSRDIPFDKVVKMDVDRFATYELDELFDEQDHIIMNQTMEEEGHLLNVVAV
                     TQEVMTELDKIRIKEFVQDVAKYDEVYGLTKKGSKQFILISENDSDEKKPHIMPVWSI
                     KNRALKVRDEDFEECDLITIEGSVFGEWLDELRDDHKAVAIDLKTGVVGTIVSAQKLS
                     NELTF"
     gene            complement(6666..7469)
                     /gene="coaW"
                     /locus_tag="P6L01_001853"
     CDS             complement(6666..7469)
                     /gene="coaW"
                     /locus_tag="P6L01_001853"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II pantothenate kinase"
                     /protein_id="HDP3030919.1"
                     /translation="MKVGIDAGGTLIKIVQEQDNQRTFKTELTKNIDQVVEWLNQQQI
                     EKLCLTGGNAGVIAENINIPAQIFVEFDAASQGLGILLKEQGHDLADYIFANVGTGTS
                     LHYFDGQSQRRVGGIGTGGGMIQGLGYLLSQITDYKQLTDMAQHGDRNTIDLKVRHIY
                     KDTEPPIPGDLTAANFGHVLHHLDADFTPSNKLAAVIGVVGEVVTTMAITVAREFKTE
                     NIVYIGSSFHNNALLRKVVEDYTVLRGCKPYYVENGAFSGAIGALYLGK"
BASE COUNT         2665 a         1086 c         1532 g         2440 t
ORIGIN      
        1 aacttcaaca ggttttgcag gtggcggtgc gactgcagaa gacgttaaat taatgaaaga
       61 tacagtaggt gctgatgtag aagtaaaagc atcaggtggc gtacgtaatt tagaagattt
      121 caataaaatg gttgaagcag gtgcgacacg tattggtgcg agcgcaggcg ttcaaattat
      181 gcaaggttta gaagcagatt cagattacta attttataaa aatatgtaag catgggacat
      241 cgtaaccata tataagtatg tcgatatcag ctttaagaca tcataaatgc catgatgatt
      301 ctaagataat ttaataggga ctttaacgtt ataaccaatc tatttaagaa taataacatc
      361 gcattgaaat tgtgatatta aaaatgaaat cgatagcttg ctataataat tttcgcttgt
      421 gcttttaaaa atggactaga gaaagcgcag atgctttgtc ctaagctttt taagaaaagg
      481 atgataaaaa tgagaatgat agacattatt gagaaaaagc gtgacggtca tacactaaca
      541 acggaagaaa ttaatttctt tattgacggc tatgttaaag gggatattcc tgattaccaa
      601 gcatcaagtt tagcaatggc gatttatttc caagatatga atgatgatga gcgtgcagca
      661 ttaacgatgg ctatggttaa ttctggtgat atgatagatt tgagtgatat taaaggtgtc
      721 aaagtagata agcactcaac aggtggtgta ggagatacaa ctacattggt tttagcacca
      781 ttagtagcag ctgtagatgt tcctgttgca aaaatgagtg ggcgtggatt aggtcataca
      841 ggtggtacga ttgataaatt agaagcaatt gatggttttc atgttgaaat agatgaagca
      901 acatttgtga aattggttaa tgaaaataaa gtggcagttg taggacaatc aggaaattta
      961 actcctgcag acaaaaaatt atatgcctta agggatgtta ctggtactgt caattcaata
     1021 ccattaattg cctcttcaat tatgagtaaa aagattgctg ctggtgcaga tgcaattgta
     1081 ttagatgtaa aaactggtag cggtgcattt atgaaaacat tagaagatgc tgaagcatta
     1141 gcgcacgcaa tggtacgtat tggtaataat gtgggacgta atacgatggc gattatttct
     1201 gatatgaatc agccacttgg acgtgcgatt ggcaatgcgc ttgagttaca agaagcaatt
     1261 gatacgttga aaggacaagg tccgaaagat ttaacagaac ttgtattaac attaggttct
     1321 caaatggttg tacttgcaaa caaagctgaa acgcttgaag aagcgagagc gttgctaatt
     1381 gaggcaatta attcaggtgc agcattagaa aaattcaaaa catttattaa aaaccaaggt
     1441 ggcgacgaaa ctgttattga ccatccagag cgtttgccac aagctcaata tcaaattgaa
     1501 tataaagcta aaaaatcagg ttatgtgact gaattagtct ctaacgatat aggtgtcgct
     1561 tcgatgatgt taggagcggg acgtttaaca aaagaggatg atattgattt agcggttggt
     1621 attgttttaa ataaaaaaat tggtgataaa gtagaagaag gagaatcatt attaacgatt
     1681 cacagtaatc gccaagatgt agacgatgtt gttaaaaaat tagattcaag cataacaatt
     1741 gctgatcatg ttgtatcgcc tacattaatt cataaaatca ttacagagta ggagttgaag
     1801 ctgattgaac atactaagat atttattagg aataggtttt agtgtaatag gtgtattgca
     1861 ttttacacga gaacgacaat ttagaaatat cataccgaaa tgtttgccac ttcgaaaaac
     1921 agcggttctt gttacaggaa tctttgagat attatttgga cttgcacttt taattaaaag
     1981 accatctcaa tgcttgaaaa atacaatcaa tctattcttg ctagctgtat tcccagctaa
     2041 tatctatatg gcagttaaaa agttaccttt aggagataaa caattgccaa catggcaact
     2101 atatttaagg ttacctatgc agttcgtgtt aatgggacta attaagaaat tatagtggtt
     2161 ctcaaataaa tcggacaaat gttaaagatt ttactttttc atttgttcga ttttttgact
     2221 ttgaagcata ataaaaagca gttggacaat ggcgattgta acatcattgt tcgactgctt
     2281 ttctgattaa cttgattgac taagatttat tttcctgtac cgaaaacatc atgccattcg
     2341 tttcgtttgt ctagaaatgc ttgagcgata gttttagcac cttctaatga atgacttgct
     2401 gcccagccac attgtacttc attacaagca ggcacttcag tagcatttag cacatcattt
     2461 aaagttgctt caacaatatt taatacatca tcataattat catgattaat aaatgataca
     2521 tagaaaccag tttggcaacc cataggactt aaatcaacaa ctttgtcact atgatttcta
     2581 atattttcag ccattaaatg ttctaatgaa tgtagtcctg gcatatccat atgttctttg
     2641 tttggttgtt tgaaacgaat gtcgtatttg tgaatgacat ctccgtttaa tccttccatc
     2701 gttcccgcta atctaataaa tggggcaacc actttagtat gatctaaatt gaaactttca
     2761 acattcattt ttgtcatttt gaatttcctc ctatttacta ctcaaaacta taaaacgctt
     2821 atgatttgtt gcaatataaa acttaagttt gttgtaagtt gtgtttctaa caatacttaa
     2881 attgtaacaa tagggcttat gttttacaat ttgaaactgt tttataaatt tatgaaaggc
     2941 ttttagttat tagaaaattt tgtaaataaa gaaaaactag atgacatcat gagataaaac
     3001 cgatagaata agtaataatt cattttgaaa gaacaaaata ttgtggatga ttaaaatgag
     3061 tgtagggtaa aagggagata gtaaaaaatg agtgaaaaac aacaaattct cgattatata
     3121 gaaacaaata aatatagtta tatcgaaatc agtcatagaa ttcatgaacg tcctgaactt
     3181 ggtaatgaag aaatatttgc gtctcgaact ttaatagatc gtttgaaaga gcatgatttt
     3241 gaaattgaaa ccgagattgc tgggcatgca actgggttta tagcgacata tgattcaggt
     3301 cttgacgggc cagctatagg ttttttagca gaatacgatg ctttgccagg gttgggtcat
     3361 gcttgtggtc ataatatcat tggaactgca agtgtgcttg gtgcaattgg tttgaagcaa
     3421 gtgattgacc aaattggtgg taaagtagtc gttcttggat gtccagctga agaaggtggg
     3481 gaaaatggta gcgctaaagc ttcttatgtc aaggctggtg tgattgatca aatagacatt
     3541 gccttaatga ttcatccggg aaatgaaact tataaaacga ttgatacttt ggcagtggat
     3601 gttttagatg ttaaatttta cggaaaaagt gctcatgcct ctgaaaatgc agatgaagcg
     3661 ttaaatgcat tagacgctat gattagttat tttaatggtg tagcacaact acgacaacat
     3721 attaaaaaag atcaacgtgt gcatggtgtg attttagatg gcgggaaagc agctaatatt
     3781 attccagact atacacatgc tcgtttttat actagagcaa tgacgcgtaa agaattggat
     3841 atattaacag aaaaagtaaa tcaaatcgca cgtggagctg cgatacagac tggttgtgat
     3901 tatgaatttg gtccaattca aaacggtgtg aatgaattca ttaaaacgcc gaaattagat
     3961 gatttatttg ctaaatatgc tgaagaagtt ggtgaagcag ttattgatga tgattttggt
     4021 tatggctcta cggatacagg gaacgtaagt catgttgtgc caacaataca tcctcatatt
     4081 aaaataggat cacgtaattt agtaggacat acgcatagat ttagagaggc ggctgcgagt
     4141 gtacatggtg atgaagcatt aattaaaggt gctaaaataa tggcgttgat ggggttagag
     4201 ttaattacaa atcaagacgt ttatcaagac attattgaag agcatgcgca tttgaaaggg
     4261 aatgggaagt aatgacaaat aatggtaatg agccgaactt aacgttaagt gatttatatg
     4321 acaaagatgt tgtctatacg tcaagacctt cgtatatatc gaatccatgg ttgaaaccgg
     4381 acgaacatca atctaatttt ttaactggta gggagttatt aatagcaaat caattacctg
     4441 ttattgttca cgaagctagt gcgacagaca aattgcatca gctatttcaa gttattggta
     4501 aggaagtacc gaactcaatt tttacattta acaatcagca atcttacgaa aacttgatca
     4561 aacaattggc acataaggaa aataaaaaga tttattttca atatattcat gatgaaacaa
     4621 tcttgaatca gcaatattat gcattagata aaacgttatt tgttgcttta aataataaag
     4681 caagaatacc tgaatggaca aatgggaagt ttttaccaaa aagaaaagtt gtaaaaattg
     4741 agcaattcga aaacgagatt aagaattggg aattcccgtt agtaattaag ccgggagatg
     4801 atcttccgac agcaggtggc tatggcgtta tgatttgtta tcatgatgca gatttacaaa
     4861 aagcgataac gcgtattaaa gaggcaactg ctgaaacgaa ttcgcttatt atcgaacaga
     4921 agattgaaga aaaagcgaat tattgcgttc agtttgcata ctcggaatcg ctaggcattc
     4981 aatatcttgg tgcggcaacg caattaacgg ataagtacgg attttataac ggaaatgaaa
     5041 atactactaa tgtaccagag cacgttatag aagcaggtag acaaattatg gaaaacggtg
     5101 tgaatcaagg attctttggt gtagctggtt ttgacctact cgtcgatgag gatgataacg
     5161 tttatgcgat tgatttaaac tttagacaaa atggttcaac gagcatgtta ttacttgcta
     5221 acgagttgaa ttcaggatat caaaagtttt atagttatca ttcaaaaggt gataacacac
     5281 atttcttcaa tacgattttg aaatatgtca aagaaggtag tttatacccg ttatcttatt
     5341 atgatggtga ttggtacggt gaagataaag ttaaatcaag gtttggctgt atttggcatg
     5401 gtgattcaaa agaaacagta ctggagaatg aacgcgcatt tttagctgaa cttgaacact
     5461 attagagttc ggaacataag gcgctacaat gttgtgttgc cagtagttga ctgaatatgc
     5521 gtttgtaaca agcttttttc gattctagtc aacagtaatt aaatttatga tatggcaata
     5581 ctttgtaata ctaatattaa atggcgactt ttatttcact atgttataag agttgccatt
     5641 ttgttgataa aggtatacta aaggttatcg ttttgaaatt tttagtaact agatatgttt
     5701 cgtgttatag accgaatttg tgtatacgta aaatttaatg ctattgaatt tttaaaatga
     5761 aaaacatgac attaaattga attcataata tgtctaattg actaacttgt tggagtcatt
     5821 tactatttta tgtatgacat attttaaaaa gtgagggtca agcatgtctt ataaagcata
     5881 tccattcttt agagatatat taataaatga atgtatttat ttcgcctcta aaaataaaaa
     5941 actagtacgc ctaaattata aaagtgaagc gtatgtaggc gtttggacag aagaaagtgt
     6001 ggccgtatca tttttaacaa gtcgtgatat tccatttgat aaagttgtaa aaatggacgt
     6061 tgatcgtttt gctacttatg aattagatga attgtttgat gaacaagacc atattattat
     6121 gaatcaaaca atggaagaag aagggcatct actaaacgtt gtagctgtta cacaagaagt
     6181 gatgacggaa ttagataaaa ttagaatcaa agaatttgtc caagatgtag caaaatatga
     6241 tgaagtatac ggcttaacta aaaaaggtag taagcagttt attctcatta gtgaaaatga
     6301 tagcgacgaa aaaaagccgc atattatgcc tgtatggagt attaaaaaca gagcgttaaa
     6361 agttcgagat gaagattttg aagagtgtga tttaattacg attgaaggtt ctgttttcgg
     6421 agaatggcta gatgaactta gagatgatca taaagccgtt gcaatagatt taaaaactgg
     6481 cgtggttggt acaattgttt cagcgcaaaa attgtctaat gaacttacat tttaattgaa
     6541 ttgagttgta agtaattaag tgataataaa tggaacaata cgtattccaa aacacgtaga
     6601 ccataaaata aaaggccatt tatatagcgt ttatttaaaa caacgcgcat ataaatggtc
     6661 tttttctatt tacctaaata taatgcacca atagcacctg aaaatgcgcc gttttcaaca
     6721 tagtacggtt tgcaaccgcg taacacagta taatcttcca caactttgcg taataaagcg
     6781 ttattatgga atgaagaacc gatataaacg atattttcag ttttaaattc acgtgcaaca
     6841 gtaatggcca ttgtcgtaac aacttcgcca acgacaccaa taacggctgc taatttattg
     6901 ctaggtgtaa aatcagcatc taaatgatgt agtacatgac caaaattagc tgctgttaaa
     6961 tcaccgggaa tgggtggttc ggtatcttta taaatatgtc taacctttaa atcgatagtg
     7021 ttacgatcac cgtgttgtgc catgtcagtt aactgtttat aatcagtgat ttgacttagt
     7081 aaataaccga gtccttgaat catgcctcca cctgtaccga taccgcctac acgacgttgt
     7141 gattggccgt cgaaataatg tagtgacgta ccggtaccaa catttgcaaa aatataatct
     7201 gctaagtcat ggccttgctc ttttaacaaa atacctagtc cttgagatgc agcatcaaac
     7261 tctacaaaaa tttgtgcagg aatgttgatg ttttcagcaa tgacacctgc attacctcca
     7321 gttaagcata atttttcaat ttgctgttgg tttaaccatt ccacaacttg atcaatattt
     7381 ttagttaatt cagttttaaa agtacgttgg ttatcttgct cttgaacgat tttaattagt
     7441 gtaccgccag cgtcaatgcc aactttcata agattcccac ctcattatta atgtctatcc
     7501 ttaaataata gtatagtaaa atgactaaaa aacaagtaat aatagtaatt attaacaaat
     7561 ttgatgccat tgtatttcga tattgtaagc gcatcgcaat taatgtatta caaacgtaga
     7621 cgttaagtta tatatattat tttctaggaa ttttgaagtt gtataggatt gttagttagt
     7681 gacgcaatat taaaagtagt tcgtacgcag tgtatttgta agt
//
LOCUS       DAOBBH010000119         7634 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.119, whole genome shotgun sequence.
ACCESSION   DAOBBH010000119 DAOBBH010000000
VERSION     DAOBBH010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7634
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.119"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            120..1622
                     /locus_tag="P6L01_001854"
     CDS             120..1622
                     /locus_tag="P6L01_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDP3030920.1"
                     /translation="MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVP
                     FQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMKSYL
                     SMFTKKYPNISTNYNKVQEIYTNSNIDETNLTYYICTTNSEQSWQAYDYVFLTCGTFA
                     YHDPYNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPI
                     TMTSRSAHLPSVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTIVSLFLKECAEYDI
                     DFKKLVHRRTGNHIADLKYDLARPTEMGIFQSMIEHLKENLNWIWNSLSIEDQHQFNQ
                     KYSKMIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDM
                     YNVVINATGAKSHLNELDQDDQLIKNLENRQIVQAHPMGGIQIIPETNQVISPRFGTL
                     TNMIAIGQMTNGVNKLRNGVKMIVEQVAHTVSQLYDALESNEQQQRSYNQ"
     regulatory      1841..1976
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            2103..3146
                     /gene="ribD"
                     /locus_tag="P6L01_001855"
     CDS             2103..3146
                     /gene="ribD"
                     /locus_tag="P6L01_001855"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDP3030921.1"
                     /translation="MSQFMDYAIQLANMVQGQTGVNPPVGAVVVNEGRIVGIGAHLRK
                     GDKHAEVQALDMAQQNAEGATIYITLEPCSHFGSTPPCVNKIIDCKIAKVVYATKDNS
                     LDTHGDETLRAHGIEVECVDDERASQLYQDFFKAKAKQLPQITVKVSASLDGKQANDN
                     GQSQWITNKEVKQDVYKLRHRHDAVLTGRRTVELDDPQYTTRIQDGKNPIKVILSKSG
                     NIHFNQQIYQDESTPIWIYTENPNLTSNQTHIEIIYLKSCDLTTILHNLYKRGVGTLL
                     VEAGPTTTSEFLQSNYIDEFILYYAPKLIGGSGNYQFYQTNDVIEIPDANQFEIVHSE
                     LLNQNVKLTLRKK"
     gene            3153..3785
                     /locus_tag="P6L01_001856"
     CDS             3153..3785
                     /locus_tag="P6L01_001856"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDP3030922.1"
                     /translation="MFTGIVEEIGVVKSVQIRQSVRTIEIEAHKITADMHIGDSISVN
                     GACLTVIDFDQTSFTVQVIKSTENKTYLADVKRQSEVNLERAMSGNGRFGGHFVLGHV
                     DELGTVSKINETANAKIITIQCSQHINNQLVKQGSITVDGVSLTVFDKHDNSFDIHLI
                     PETRRSTILSSKKLGDKVHLETDVLFKYVENILNKDKDQLSVDKLRAFGF"
     gene            3796..4977
                     /gene="ribB"
                     /locus_tag="P6L01_001857"
     CDS             3796..4977
                     /gene="ribB"
                     /locus_tag="P6L01_001857"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HDP3030923.1"
                     /translation="MQFDNIDSALMALKNGETIIVVDDENRENEGDLVAVTEWMNDNT
                     INFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYE
                     RTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
                     ICEIMNDDGTMAKGQDLQNFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFG
                     TFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMK
                     YINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQ
                     ILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMVTKKIKMGHL
                     I"
     gene            4990..5454
                     /locus_tag="P6L01_001858"
     CDS             4990..5454
                     /locus_tag="P6L01_001858"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDP3030924.1"
                     /translation="MNFEGKLIGKDLKVAIVVSRFNDFITGRLLEGAKDTLIRHDVNE
                     DNIDVAFVPGAFEIPLVAKKLASSGNYDAIITLGCVIRGATSHYDYVCNEVAKGVSKV
                     NDQTNVPVIFGILTTESIEQAVERAGTKAGNKGAEAAVSAIEMANLLKSIKA"
     gene            complement(5576..6577)
                     /locus_tag="P6L01_001859"
     CDS             complement(5576..6577)
                     /locus_tag="P6L01_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HDP3030925.1"
                     /translation="MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPELIN
                     TIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLG
                     AEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVI
                     QAYLYDSHKLVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNF
                     TSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYG
                     DDWFAYFMRRLAERPQNLSLAVKEFVKPAGLKRVGIIAALGATVMLGLSTIKKLCRK"
     gene            6689..6808
                     /locus_tag="P6L01_001860"
     CDS             6689..6808
                     /locus_tag="P6L01_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030926.1"
                     /translation="MMLISFIYNNMDNNNTINRNLHSEFAIIMLLKINEKGAV"
     gene            6811..>7634
                     /locus_tag="P6L01_001861"
     CDS             6811..>7634
                     /locus_tag="P6L01_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDP3030927.1"
                     /translation="MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMG
                     DLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEK
                     VELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVE
                     ETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPMPTLLMYGTKDLIVDTRAIDEF
                     KEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVE"
BASE COUNT         2743 a         1064 c         1416 g         2411 t
ORIGIN      
        1 tggttgtgaa agtgaaataa tagcttacct taataaattt tattggaaat accaaattgc
       61 tataattata ggtaatagtt taaatataat attttgaatt acaaaaggat ggatgatata
      121 tgcgtgtagc aataataggc atgggaactg ctggtgtaag tgtattgaga gagttagtta
      181 agcacccgaa gtttaatcaa ttagatatag atttatacga tgataaagta aatatgggac
      241 agggcgtacc atttcaaaat gatagttctg agctgctaat taatatgcct tcgaaaaaga
      301 tgagtttaaa tttagatgat gagactgaat tttggaagtg gtataaacaa caaactgatt
      361 ttaattttga tgaacctgca tatttaccga gatttgtatt cggacattat atgaaatctt
      421 atttatctat gttcacgaaa aaatatccaa atatatcaac taattataat aaagttcaag
      481 agatttatac aaattctaat atcgatgaaa cgaatttaac ttattacata tgtactacaa
      541 attcagaaca atcatggcaa gcgtacgatt atgtattttt aacatgtggt acatttgcct
      601 accatgatcc atataactta aagggtaaaa aaggatatat cgctacgcct tatccaacat
      661 ataatacgtt agacgaagta aatgaactgg acgatattgc gattattggt acaggccttg
      721 ccagtttaga tgttgtccgt tatgttgctg ctcatcatcc gaaattacca attactatga
      781 cgagtcgttc tgctcattta ccaagtgtaa gaggtacaat gatagacgtg acattcaagt
      841 acttaactaa agacaaatta aatgacatta aaaaacatca tttcggcaat gcaccgcttg
      901 atactatagt ttctttattt ttaaaagaat gtgctgaata tgacattgat tttaaaaaat
      961 tagtacatag acgtacagga aatcatattg cagatttgaa gtatgattta gcgcgtccaa
     1021 cagaaatggg cattttccaa agtatgattg aacatttgaa agagaattta aattggatat
     1081 ggaatagctt aagtattgaa gatcaacatc aatttaatca aaaatactca aagatgattc
     1141 aattaaattc aaatccaatg cctccaagaa ctgctgaact aattattgaa ttaatagaaa
     1201 ataagtcttt aattttgaaa aaagatttag aagatgtgaa acatgatggc aagttgtatt
     1261 atttttcata caaaaaccaa gaatcagtag acatgtataa tgttgtcatt aacgcgacag
     1321 gtgcaaaaag tcatttaaat gaattggatc aagatgatca attaattaaa aatcttgaaa
     1381 atagacaaat tgtacaagca catccaatgg gaggcataca aatcataccg gaaaccaatc
     1441 aagttattag tccaaggttt ggaacattaa caaatatgat tgcgataggt caaatgacaa
     1501 acggtgttaa taaactacga aatggtgtga aaatgatagt agaacaagtt gcgcatacag
     1561 tatctcaatt atatgatgct ttagaatcga atgagcaaca acagcgcagt tacaatcaat
     1621 aatttgtaac tagaagataa taaagagaac gctctataga gacgaattga aggtttgatt
     1681 ttaatgtctg ttagtaagaa tcatatcaat gagatgccta tagtactcag attatattaa
     1741 attaaaaccg tcattaattg ttttttcaga aaacatatag tatcatttta aatgtagttg
     1801 acatactacg tactcaaata atctataaca atttcatata taattctttc ggggcagggt
     1861 gaaattccca accggcagta aataaagcct gcgacctgct aatatgtttc atattagtgg
     1921 ctgatctagt gagattctag agccgacagt taaagtctgg atgggagaaa gaatgttaat
     1981 tatcgacaaa gataatgtag cgtatttgta aaaatgtgta caaataggct tatttaacga
     2041 taaatttttc tccttgcatc ttaattcatg atgtgaggaa ttttgtttat agaggtgatc
     2101 atttgagtca atttatggat tatgcgattc aacttgcaaa tatggtacaa ggtcaaacag
     2161 gtgttaatcc acccgttggc gctgttgtag ttaatgaagg taggattgtt ggtattggtg
     2221 cacacttgag aaaaggtgac aagcatgcgg aggttcaagc acttgatatg gcacaacaaa
     2281 atgctgaagg tgcgacgatt tatattacgt tagagccatg tagtcatttt ggttcaacac
     2341 caccctgtgt taacaaaatt attgattgta agatagcaaa agtagtatac gcaacaaaag
     2401 acaattcgtt agacacacat ggtgatgaga cgttacgggc tcacggtatt gaggttgaat
     2461 gcgttgatga tgaacgggca tcacaattat accaagactt ttttaaagca aaagcaaagc
     2521 aactgccaca aattacagtg aaagtatctg caagtttaga tggtaaacaa gcgaatgata
     2581 atggacaaag tcaatggatt actaacaaag aggttaaaca agatgtctat aagttaagac
     2641 atcgacacga cgcagtgtta actggaagac gtacagttga attagatgat ccacaatata
     2701 ctacacgtat tcaagatgga aaaaacccta taaaagtaat attgtctaag tctgggaata
     2761 ttcattttaa tcagcaaatt tatcaagatg aatcaacacc aatttggata tatactgaaa
     2821 atccaaattt aacaagcaat caaacacata ttgaaattat ttacttgaag tcttgtgatt
     2881 taacaacaat tcttcacaat ttatataaaa gaggagttgg aactttgcta gtcgaggcag
     2941 gtccaaccac tacttcagaa tttctccaat ctaattatat agatgaattt attctctatt
     3001 atgccccgaa attaattggc ggatctggaa attatcaatt ttatcaaaca aatgatgtga
     3061 ttgagatacc agatgcgaac caatttgaaa ttgttcattc cgagttatta aatcaaaatg
     3121 ttaaattaac tttacgaaag aagtgatgat gcatgtttac tggcatcgtt gaagaaatag
     3181 gtgtcgttaa aagtgttcaa attcgtcaat cagtcaggac gattgaaatc gaagcacata
     3241 agattacggc agatatgcat attggtgatt ccatcagtgt taatggtgca tgtttaacag
     3301 tgattgattt tgatcagaca tcttttactg ttcaagtaat taaaagcact gaaaataaaa
     3361 cctatttagc agatgttaag cgacaatcag aagtaaattt agagcgtgcc atgagtggta
     3421 acggtaggtt tggtggacat tttgtcctcg gtcatgtaga tgaactagga acagtttcaa
     3481 aaataaatga aacagccaat gccaaaatta ttacgattca atgtagccaa catattaata
     3541 atcagttagt taagcaaggg tctattactg tggatggtgt aagtctaacg gtatttgata
     3601 agcatgataa cagttttgac attcatctta ttccagaaac gaggcgttca acgattttat
     3661 catccaaaaa attaggagat aaagtacatt tagaaacaga cgttttgttt aaatatgttg
     3721 aaaatatttt aaataaagat aaagaccaat tatctgtaga taaattaaga gcatttggtt
     3781 tttaggaggg gtagcatgca attcgataat attgacagtg ctttaatggc tttaaaaaat
     3841 ggagaaacaa ttattgtagt agatgatgag aatcgtgaaa atgaaggtga tttagtagcg
     3901 gttactgaat ggatgaacga taataccatt aattttatgg cgaaagaagc aaggggatta
     3961 atatgcgcac cagtgtctaa agatattgca caacgtttgg atttggtaca aatggttgat
     4021 gataactccg acatctttgg tacgcaattt acagtgagta ttgatcatgt agatacaaca
     4081 acaggaatta gtgcttatga acgtacattg actgccaaaa agctcattga tcctagtagt
     4141 gaagctaaag attttaatcg tcctggtcat ttatttccat tagtagcaca agataaaggc
     4201 gtattagcta gaaatggaca cacagaagcg gctgttgatt tagctaaact tactggtgcc
     4261 aagcccgctg gtgtcatttg tgagattatg aatgatgacg gcacgatggc gaaaggacaa
     4321 gatttacaaa attttaaaga aaaacatcaa ttaaagatga ttacgattga tgatttaatt
     4381 gaatatcgta aaaaattaga accagaaatt gaatttaagg caaaagtgaa aatgcctaca
     4441 gatttcggaa catttgatat gtatggtttt aaagcgacat acacagatga agagatagtt
     4501 gtactgacaa aaggtgcaat tcgacaacat gaaaatgtac gcttacattc tgcgtgcctt
     4561 acaggcgata ttttccatag tcaacgttgt gattgtggtg ctcaacttga atcgtctatg
     4621 aagtatatca atgaacatgg tggcatgatt atttatctac ctcaagaagg tcgtggcata
     4681 ggattgttaa acaaattacg cgcatatgaa ttaattgagc aaggatatga tacagtaact
     4741 gcaaatttag cattaggttt tgatgaagat ttacgagatt atcatattgc tgcacagatt
     4801 ttaaaatatt ttaacatcga acatatcaat ttattaagta ataatccaag taaatttgag
     4861 ggattaaaac aatatggcat tgatattgca gaaagaattg aagttatcgt accagaaacg
     4921 gtacataatc atgattatat ggtaacgaaa aaaataaaaa tgggtcattt aatataggag
     4981 gactttaaca tgaattttga aggtaaatta attggaaaag atttgaaagt tgcaatcgta
     5041 gttagtcgat ttaatgattt tatcactgga agattacttg aaggtgcaaa agatactttg
     5101 attcgacatg atgttaatga agacaatatt gatgtagcat ttgttcctgg tgcgtttgaa
     5161 attcctttag tagctaaaaa attagcctca tcaggaaatt atgatgcaat aattacatta
     5221 ggatgcgtaa ttcgcggtgc tacgtctcat tatgattatg tttgtaatga agtcgcgaaa
     5281 ggtgtttcta aagtaaatga tcaaactaat gtaccagtca tatttggtat tttaacgact
     5341 gaaagtattg aacaagctgt ggaaagagca ggtacgaaag ctggtaataa aggtgccgaa
     5401 gcagcagtaa gtgcaattga aatggctaat ttattaaaat ctataaaagc atagagcgct
     5461 gttgtgagtt cataatcaaa gatttataaa tgtttcagaa ctgccaatat aacatttagg
     5521 agcctagaac attgattatt gttccagact cctaaattat tgtttatgcc atgctctatt
     5581 tacggcataa ttttttaatt gtacttaaac ctaacataac tgtagctcct aaagctgcaa
     5641 ttatgccaac acgttttaag ccagcaggtt tcacaaattc ttttacagca agagataggt
     5701 tttgtgggcg ttctgctaat cttctcataa aatacgcaaa ccaatcatcg ccataaggta
     5761 cataaatagt gaaattatag ccttcatttg cgatttcttc tgctaactct gatctaaaac
     5821 catagagcat ttggaattcc atacgatctt tttcaatgtg attttctttc ataaattgtt
     5881 ttacatgatt aatgatgcga tggtcatgtg ttgcaattga agtgaaattg cgtgcgttta
     5941 acaaacgttg ttcaattatt ttgatgtaat ttgcatctac gtcttcctta gattgaaatg
     6001 caattgattc gttttcttta tatgcacctt taaccaaacg taatcgtaaa tcttggtact
     6061 tatcaactaa tttgtggcta tcgtataaat atgcttgaat tacagtacca acatttctaa
     6121 attcgccttt taagcgatct aaaacttgaa caatttgttg caggctagca tatttttcag
     6181 tatcaatatt tatatgcata ttgttgtaag tatttgcttt aagtaaaatt tctcttaaat
     6241 tttggtaagc taattctaag tcgaattctg cacctaactg actcaattta acagacatat
     6301 gtgcctttac gccatgttga tgaagcgcgt ccataattgt taaaatttgt tctttagcat
     6361 gattactttc ttcaactgta ccgacaaatt cccctaaatt gtctaccgta acagcgatat
     6421 tcttgtcatt taagtattcg attgtattaa ttaactctgg aattgtattt ccggcaacga
     6481 ctttattggc gcccaaacgt gggcccactt tttttgctgc gttgtttaaa aaactattat
     6541 tagataatcc gataaaaaaa ttctttaata gtgccattgc tactcctcca aacccttgtg
     6601 tttacaataa gtttagcatg aaaaaaatat tcgagaaagc gttttcataa aaaacaatca
     6661 aaaataatta tgtataatta cactattatt gatgttgatt tcatttatat ataataatat
     6721 ggacaacaat aatactataa atagaaatct acatagtgaa tttgctataa ttatgttatt
     6781 gaagattaat gaaaaagggg cagtataata atgtggaagt gggaagctga aaatgatgcg
     6841 aaaggcgtcg ttgttattgc tcataatata ttagaacata cgggaagata tgcatatgtt
     6901 attacgatgt taagaagaaa tggctatcat gtgattatgg gagatttgcc tggtcaaggt
     6961 caaacttcaa gagctaataa aggacaaatt gaaaactttc aaacgtatca cgaaagtttg
     7021 ctagattggt taaagatagc taatgaatat aaaattccta cttatgtatt aggggtagga
     7081 cttggcggtt tgattttatt aaatttactt gaaaaagtag aattgccaat cgaggggatg
     7141 atgttaattt ctcctatgct agaacttcaa aagaatggaa aaaatcgtaa agataaactt
     7201 gtttcaaata taggtaaaat ttcaaaagac acacgtttta atgttggtgt agaaccaaaa
     7261 gatttaacac gtaatttaga aattgttgaa gaaacagtta acgatggttt aatgttaaaa
     7321 aaagcaacgt atcattggta taatacaatt aatgagacga tgaaagatac gatggctcat
     7381 attcatgaca ttcaaccaat gccaacgcta ttaatgtatg gaacgaagga tttaattgtt
     7441 gatactaggg caattgatga gtttaaagaa aaatatcaaa cacctgaatt atattttaaa
     7501 gcttggcaag gtttttacca cgaggttcac aatgagccag aacgtgatga agtaatgcgt
     7561 tatattctga ctttcttaaa taacagcgtc aatacaatgg gctttattgt tgaagatgac
     7621 gaaattgtag aaat
//
LOCUS       DAOBBH010000120         7483 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.120, whole genome shotgun sequence.
ACCESSION   DAOBBH010000120 DAOBBH010000000
VERSION     DAOBBH010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.120"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(58..133)
                     /locus_tag="P6L01_001862"
     tRNA            complement(58..133)
                     /locus_tag="P6L01_001862"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(98..100),aa:Ala,seq:tgc)
     gene            complement(157..230)
                     /locus_tag="P6L01_001863"
     tRNA            complement(157..230)
                     /locus_tag="P6L01_001863"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(194..196),aa:Pro,seq:tgg)
     gene            complement(251..327)
                     /locus_tag="P6L01_001864"
     tRNA            complement(251..327)
                     /locus_tag="P6L01_001864"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(291..293),aa:Arg,seq:acg)
     gene            complement(333..421)
                     /locus_tag="P6L01_001865"
     tRNA            complement(333..421)
                     /locus_tag="P6L01_001865"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:taa)
     gene            complement(429..503)
                     /locus_tag="P6L01_001866"
     tRNA            complement(429..503)
                     /locus_tag="P6L01_001866"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(469..471),aa:Gly,seq:gcc)
     gene            complement(537..609)
                     /locus_tag="P6L01_001867"
     tRNA            complement(537..609)
                     /locus_tag="P6L01_001867"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(574..576),aa:Lys,seq:ttt)
     gene            complement(616..691)
                     /locus_tag="P6L01_001868"
     tRNA            complement(616..691)
                     /locus_tag="P6L01_001868"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(656..658),aa:Thr,seq:tgt)
     gene            complement(708..783)
                     /locus_tag="P6L01_001869"
     tRNA            complement(708..783)
                     /locus_tag="P6L01_001869"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Val,seq:tac)
     gene            complement(796..910)
                     /gene="rrf"
                     /locus_tag="P6L01_001870"
     rRNA            complement(796..910)
                     /gene="rrf"
                     /locus_tag="P6L01_001870"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(1468..2955)
                     /gene="lysS"
                     /locus_tag="P6L01_001871"
     CDS             complement(1468..2955)
                     /gene="lysS"
                     /locus_tag="P6L01_001871"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDP3030928.1"
                     /translation="MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEW
                     DQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEF
                     DLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
                     QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTH
                     HNALDAMLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAY
                     ADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFY
                     EVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLA
                     KKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDE
                     DYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK"
     gene            complement(3008..3100)
                     /locus_tag="P6L01_001872"
     CDS             complement(3008..3100)
                     /locus_tag="P6L01_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030929.1"
                     /translation="MVNDKVLETSKEMYVEQKCLIFYKTLKENV"
     gene            complement(3494..3970)
                     /gene="folK"
                     /locus_tag="P6L01_001873"
     CDS             complement(3494..3970)
                     /gene="folK"
                     /locus_tag="P6L01_001873"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDP3030930.1"
                     /translation="MIQAYLGLGSNIGDRESQLNDAIKILNEYDGISVSNISPIYETA
                     PVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEECLHRIRKERWGPRTLDVDILLYG
                     EEMIDLPKLSVPHPRMNERAFVLIPLNDIAANVVEPRSKLKVKDLVFVDDSVKRYK"
     gene            complement(3967..4332)
                     /gene="folB"
                     /locus_tag="P6L01_001874"
     CDS             complement(3967..4332)
                     /gene="folB"
                     /locus_tag="P6L01_001874"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDP3030931.1"
                     /translation="MQDTIFLKGMRFYGYHGALSAENEIGQIFKVDVTLKVDLAEAGR
                     TDNVIDTVHYGEVFEEVKSIMEGKAVNLLEHLAERIANRINSQYNRVMETKVRITKEN
                     PPIPGHYDGVGIEIVRENK"
     gene            complement(4310..5113)
                     /gene="folP"
                     /locus_tag="P6L01_001875"
     CDS             complement(4310..5113)
                     /gene="folP"
                     /locus_tag="P6L01_001875"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDP3030932.1"
                     /translation="MTKTKIMGILNVTPDSFSDGGKFNNVETAINRVKAMIDEGADII
                     DVGGVSTRPGHEMVTLEEELNRVLPVVEAIVGFDVKISVDTFRSEVAEACLKLGVDMI
                     NDQWAGLYDHRMFQIVAKYDAEIILMHNGNGNRDEPVVEEMLTSLLAQAHQAKIAGIP
                     SNKIWLDPGIGFAKTRNEEAEVMARLDELVATEYPVLLATSRKRFTKEMMGYDTTPVE
                     RDEVTAATTAYGIMKGVRAVRVHNVELNAKLAKGIDFLKENENARHNLS"
     gene            complement(5329..6261)
                     /gene="cysK"
                     /locus_tag="P6L01_001876"
     CDS             complement(5329..6261)
                     /gene="cysK"
                     /locus_tag="P6L01_001876"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDP3030933.1"
                     /translation="MAQKPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGS
                     VKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQ
                     ERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTG
                     PELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPG
                     PHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAA
                     IQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD"
     gene            complement(6440..7321)
                     /gene="hslO"
                     /locus_tag="P6L01_001877"
     CDS             complement(6440..7321)
                     /gene="hslO"
                     /locus_tag="P6L01_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDP3030934.1"
                     /translation="MTHDYIVKALAFDGEIRAYAALTTETVQEAQTRHYTWPTASAAM
                     GRTMTATAMMGAMLKGDQKLTVTVDGQGPIGRIIADANAKGEVRAYVDHPQTHFPLNE
                     QGKLDVRRAVGTNGSIMVVKDVGMKDYFSGASPIVSGELGEDFTYYYATSEQTPSSVG
                     LGVLVNPDNTIKAAGGFIIQVMPGAKDETISKLEKAISEMTPVSKLIEQGLTPEGLLN
                     EILGEDHVQILEKMPVQFECNCSHEKFLNAIKGLGEAEIQNMIKEDHGAEAVCHFCGN
                     KYKYTEEELNVLLESLA"
BASE COUNT         2267 a         1609 c         1028 g         2579 t
ORIGIN      
        1 ggaattaacg ttgacatatt gtcattcaat tttcaatgtt catcgtgtaa aaataaatgg
       61 tggagactag cgggatcgaa ccgctgacct cctgcgtgca aagcaggcgc tctcccagct
      121 gagctaagcc cccataaaat gaaatttaag aagtaatcgg gaagacagga ttcgaacctg
      181 cgaccccttg gtcccaaacc aagtgctcta ccaagctgag ctacttcccg ttattcaatt
      241 aatttaaaaa tggcgcgccc gataggagtc gaacccataa cctcttgatc cgtagtcaaa
      301 cgctctatcc aattgagcta cgggcgctaa aatggtgccg aggaccggaa tcgaaccggt
      361 acggtgatct ctcaccgcag gattttaagt cctgtgcgtc tgccagttcc gccaccccgg
      421 caaaataatg gagcagaaga cgggattcga acccgcgacc ccaaccttgg caaggttgta
      481 ttctaccgct gaactacttc tgcattattt tgctaaatat aaaaattaaa tcgtaatgag
      541 ccatagagga ttcgaacctc tgaccctctg attaaaagtc agatgctcta ccagctgagc
      601 taatggctca aaagatggtg ccggccagag gacttgaacc cccaacctac tgattacaag
      661 tcagttgctc taccaattga gctaggccgg ctatatctaa gaataaatgg tggagaatga
      721 cgggttcgaa ccgccgaccc tctgcttgta aggcagatgc tctcccagct gagctaattc
      781 tccgatttgt catttgcctg gcaacgttct actctagcgg aacgtaagtt cgactaccat
      841 cgacgctaag gagcttaact tctgtgttcg gcatgggaac aggtgtgacc tccttgctat
      901 agccaccaga caaaataact tacttattta tgatacacgc ttaattaata aggtgcaata
      961 gttaatttta cactttgttg tgaactttgt cacatcagct tttagtgtgt ttcgtctgtt
     1021 ttttacgact tttatatatt acttcattca attgataaag tcaacacttt taatcaactt
     1081 ttttattctt caaaactgac atttctcgtg ctctctaatt cataacactt ttagtattct
     1141 atcatacaca tatgtacaaa accatacaca ttctatacaa ttttaaagta cttttatagc
     1201 tgattaagat aaattatact aatatagcaa tttttgttat aagatatctc aatgtctatc
     1261 gttaacattt ctcatccgaa ctactctata tacgtatcgt cattttcatc tatcagccaa
     1321 ctaccttgtt taacagtcaa aaatagtacc actatcgtta actattacta tcaaaaacat
     1381 tcaaacacga actaattacc ataattaaga aaaccctcgc atacacatgt tgtatacacg
     1441 agagctctta ctaacaatca acgtcattta tttttgtctc atataaggga ataataacac
     1501 gtctctgatt gatggagaat cagttaataa cataaccaat ctgtcaatac cgataccaag
     1561 accacctgtc ggaggcatac catattctaa cgcttcaatg taatcttcat ccatttcatg
     1621 cgcttcatca ttaccttgcg ccttttcaac aagttgcgct tcaaaacgac ctttttgatc
     1681 aataggatca tttaattcag taaatgcatt tgcatgctct ctacctacaa tgaacaattc
     1741 gaaacgatca gtaaatctag gatcttcagg atttttcttc gctaaaggtg aaatttcagt
     1801 cggatgacca tagataaacg ttggctgaat aagtgtttct tcaacttttt gctcaaagaa
     1861 ttcatttaaa atatgaccat atttcattgt atctttaatt tcaataccat gttctttagc
     1921 taaagcttta gcttcttcat cacttttaac ttcataaaaa tccacaccag tagcttcttt
     1981 tacagcatca acaatatgca aacgagtcca agcagattct aaatcaatcg tttccccatt
     2041 gtattgtact tttgcagaac ctaatacttc attggcaata tgtctcacca tagattctgt
     2101 taaatccata atgtcatgat aatctgcata tgcttcatat aattcaatca ttgtgaattc
     2161 agggttatgt ctagttgata caccttcatt acggaatact ctaccaattt catatacttt
     2221 ttcaagtcca ccgacaatta aacgttttaa atgcaactca atagcaatac gcatgtataa
     2281 cattgcatct aatgcattat gatgtgttac aaatggtcta gcagctgcgc caccagcaat
     2341 ttggtgcatc ataggtgttt ctacttccaa gaaaccttta ttatttaaat aattacgcat
     2401 ttcttgaatg attttactac gattaataaa tgtacgagtg ctatcttcgt tcgtaattaa
     2461 atctaaatat ctttgacgat atctctgttc aatatcctgt aaaccgtgga atttatccgg
     2521 taatggtcgc aatgatttag ttagtagcgt gaatttcttc gctttaaccg ataattcgcc
     2581 agtatttgtt ttgaacatta caccttcaac accaacgata tcgcctaaat cagcattttt
     2641 ccataaatca aattcgtcat cgccaacttg atctttacga acgtaaattt gaatttgtcc
     2701 agctaagtcc tgaacgtgtg caaatcctgc tttaccttta ccacgcttag tcattaatcg
     2761 tccagctata gcgacatgac tatccgcttc tttttctacc aattcttctt tagaatactg
     2821 gtcccactct tctttcaaat cactagataa acctgaacgg tcaaatttag aaccaaacgg
     2881 gtctatacca agatcatata attcttgtaa tttttgacgt cgaaccaaca tttggtcatt
     2941 catttcttct gacataactt tctctccttt aacttgttgt aatcctttag tattccaact
     3001 ggtacactca aacattttcc tttaaagttt tataaaatat cagacatttt tgctcaacat
     3061 acatctcttt cgatgtttct aatactttat cattaaccat tacaccttaa agttatgatg
     3121 tggcatgttt tcttatattc ataacatcaa ttttatcact tttttaattt tacaagagct
     3181 tgtaaatgta ataaagcaat ttctgttttc gtttatgaat tacttattca tttaaaatac
     3241 gattatcatt aatacaaccc taagtaaatg tataaaaatt atcttccaca aacttcaaca
     3301 aaagcccaaa taaattacag caatttatca aatattgctt actttgattt tatgaaataa
     3361 cttaattcta acacatacta aatcatcata tactaattcg taatcaaatg catttagaga
     3421 taatcaaaat gcggaaacat ctccaataat caatcatcta ttcccaataa atatgaatgt
     3481 tctcaacaat acattattta tatctcttta cactgtcatc gacaaaaact aaatctttca
     3541 ctttcaattt cgaacgtggt tctacgacat ttgctgctat atcatttaat gggattaaaa
     3601 caaatgcacg ttcattcatt ctcggatgtg gcaccgacag ttttggtaaa tctatcattt
     3661 cttctccata caacaaaata tccacatcta aagttctagg accccatcgt tcctttctaa
     3721 tacggtgtaa acattcttct gtcttcaaac aacattccaa cagttgtaat actgtgagtg
     3781 ttgtttgaat ttcaacacac aaatttaaaa agttaggttg ctcagtatac ccaactggtg
     3841 ctgtttcata aatcggagaa atattagata cgctaatacc atcatattca ttcaaaatct
     3901 ttatagcatc gtttaactgg ctttctctat caccaatatt actacctaat cctaagtatg
     3961 cttgaatcat ttattctccc tcactatttc gatacctact ccatcataat gacccggaat
     4021 cggtgggttt tctttagtga ttctcacttt tgtttccatt acacgattat attgtgaatt
     4081 tatacgattt gcaatacgtt cagctagatg ctcaagtaaa ttaacggcct taccttccat
     4141 aattgattta acctcttcga acacttcacc ataatgaact gtatcaataa cattatcagt
     4201 acgcccagct tcagctaagt ctactttcaa agttacatcc actttgaaaa tttgccctat
     4261 ttcattttca gctgataaag caccatgata tccataaaag cgcatacctt taagaaagat
     4321 tgtgtcttgc attttcattc tcctttaaaa aatctatacc tttagctaat ttagcattca
     4381 actcgacatt atgaacgcgt actgctctaa cgcctttcat aataccatat gcagtcgtag
     4441 ctgcagttac ttcatctctt tcaaccggtg ttgtatcata acccatcatc tctttagtga
     4501 aacgtttccg gcttgtcgct aataaaactg gatattctgt tgcaacaagt tcatccagtc
     4561 ttgccataac ttcggcttct tcatttctag ttttagcgaa acctatacct ggatctagcc
     4621 aaattttatt tgaaggtata ccagctattt tagcttgatg tgcttgtgct aacaaagatg
     4681 ttaacatttc ttcgacaacc ggttcatcac gattaccatt tccattatgc attaaaataa
     4741 tttccgcgtc atatttagct acaatttgga acatacgatg atcatacaga cccgcccatt
     4801 gatcattaat catatcaacg cctaatttta aacatgcttc agcaacctca cttcgaaatg
     4861 tatcgactga aatttttaca tcaaaaccga caatagcttc aacaacaggt aatactctgt
     4921 tcagctcttc ttctaatgta accatttcat gaccgggtct cgttgaaaca cctccaacat
     4981 ctataatgtc agcaccttca tctatcatgg ctttcactct atttatagct gtttcaacat
     5041 tattaaattt tccaccatct gagaatgaat caggtgtgac gtttaatatg cccataattt
     5101 ttgttttagt catgttaacc accctttaat tatgtattgt ttaactctaa taaaatttac
     5161 tcctgcgatt tactagttat taatatgtac gtttattatc ttatcattca ctggatgttt
     5221 ttatcagaaa aatgtttaat ttaacgctca tatttttaaa attatacatg gtagaaaaaa
     5281 agagcaagtt atctcaaaaa gataactcac tcttttaaat gacattaatt agtcatcgaa
     5341 tgaatataaa ggtgttgata agtagcgttc accattactc ggcaatactg ttactactgt
     5401 tttacctttt cctaattctt ttgctttttg aatggcagca taaatcgcag cacctgatga
     5461 aatacctgct aaaatacctt cctctttagc aactcgacga gacatttcca tcgctgtatc
     5521 atttcctact ttaataatac tgtcatagat ttctgtattc aaagtgcctg gaataaatcc
     5581 agcacctaaa ccttgtaatt tatgtggacc tggctcacca ccgctcaata ctggagaagc
     5641 ctcaggctct atagcaacaa tttcgatgtt aggatattct tttttcagaa ctttacctac
     5701 accagataac gtaccaccag taccaacacc agctaggaac gcatcgatag ttttcccttc
     5761 aaattgttgt aataactcag gacctgtagt taactcatga acttcagggt tcgcagggtt
     5821 ttcaaattgt tgtggctcga agtaaccatg ttcttctttt aattctttag cttttttaat
     5881 tgcacctttc atcgcttctg atccaggcgt taaaactaat tccgcaccgt atgcttttaa
     5941 taaattacga cgctcttggc tcattgtttc gggcatagta aatactgctt tatatccttt
     6001 agcagcacat acaaatgcta aaccgatacc tgtattacca cttgttggtt ctacaattgt
     6061 gtcgccaggt ttaattttgc cttctcgctc tgctttttca atcattgcta aagcaattct
     6121 atcctttaca gaaccacctg gattttgata ttccaatttt acataaacat ctgctgcatt
     6181 gtcatctact acatttctca atttgactac cggtgtaccg ccaataattt gagtaatatt
     6241 atctactggt ttttgtgcca ttaagaacac tcctttttag ttagttgttg aaatcttagt
     6301 ttatttatcg gatatagaat tattttatca aataaattct agaaagcaaa caaaatcact
     6361 aaggattata caactattta gttctacatt tcactaaacc gctaaaaata aacattttag
     6421 cgtattgatt taaattaaat tacgctaaac tttctagcaa cacgtttaat tcttcttcag
     6481 tatatttata tttatttcca cagaaatgac atactgcttc agcaccatga tcttctttaa
     6541 tcatattttg aatctcagcc tcgcccaatc ctttaatagc atttaaaaat ttctcatgac
     6601 tacaattaca ttcaaattga acaggcattt tctctaaaat ttgcacatgg tcttcaccta
     6661 agatttcgtt tagtaatcct tctggcgtta atccttgttc aattaattta gaaactggtg
     6721 tcatttcact aattgctttt tctaattttg aaattgtttc atctttggca cctggcataa
     6781 cttgaatgat aaatcctcct gctgctttaa tcgtattatc aggatttacc aatacaccaa
     6841 gacctaccga tgaaggtgtt tgttcacttg tagcataata ataagtaaaa tcttcaccaa
     6901 gttctcctga aacaattgga cttgctccag agaaatagtc tttcattcca acgtctttaa
     6961 caaccataat agatccattt gtccctactg ctcgtcttac atcaagttta ccttgctcat
     7021 ttaatggaaa atgagtttgt ggatggtcta cataagcacg cacctcgcct ttagcatttg
     7081 catcggcaat aattcgtcca ataggtcctt ggccatctac agtgacagtt aatttttgat
     7141 cacctttcaa cattgcgccc atcatagctg ttgctgtcat tgttcttccc attgcagcag
     7201 atgctgtcgg ccatgtataa tgtctcgttt gtgcttcttg aacagtttca gttgtcaaag
     7261 cagcataagc cctaatctcc ccatcaaatg ctaatgcttt aacaatataa tcgtgtgtca
     7321 ttatttcaat ctcctctatt actctatatt ttaaaattac tttacttcat aaaatgcaac
     7381 aattgtactt attctacacc catcattcta aataatgaag taacttgttt tacaattatt
     7441 ttctgctata acaattcaat gacttaaaat ctaatacgta ttt
//
LOCUS       DAOBBH010000121         7390 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.121, whole genome shotgun sequence.
ACCESSION   DAOBBH010000121 DAOBBH010000000
VERSION     DAOBBH010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.121"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            2..793
                     /locus_tag="P6L01_001878"
     CDS             2..793
                     /locus_tag="P6L01_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDP3030935.1"
                     /translation="MALKHYKNSDSTVFNDAKALFDLNKNILLKGPTGSGKTKLAETL
                     SEVVDTPMHQVNCSVDLDTESLLGFKTIKTNAEGQQEIVFVDGPVIKAMKEGHILYID
                     EINMAKPETLPVLNGVLDYRRQITNPYTGEVIKAVPGFNVIAAINEGYVGTLPMNEAL
                     KNRFVVIHVDYIDGDILKNVIKEQSLLQDDKQIEQIIKFNEDLRTMSKQGQISEEAAS
                     IRALLDLCDLITVMPVERAIKRTIIDKLEDEREQQAIYNAVELNF"
     gene            807..2693
                     /locus_tag="P6L01_001879"
     CDS             807..2693
                     /locus_tag="P6L01_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation protein NorD"
                     /protein_id="HDP3030936.1"
                     /translation="MSDRFIKFNDEQLDAKQVMMLQDLARLLLKNEQTQVKIQKFPYY
                     NPVQNVLITSWFWSHRPSHIEMAGLKTDVMLAAYGYHMMDVQIVNEVVQDKTFKHPKF
                     YQQLFKLLEDMRVLNSIKVERPSTAKLIDLRLDTRVSYTESQIKVYRTKTQYTDLLFL
                     YLEHAFLSQDFFDIPSIHSDLDDILVNMFLYLPNFFQNQNSEDNMYLAQRIMYQVDDI
                     LKEDMLNEYYYLPKTLYNTLASPEFDDLKRTDASQVDGQDDTSEDDDNESEKADSKSA
                     DSESKGGAYLEMELHEGQNSETLGNDEAREGDATDDMTDMMTKKGKGSNDTLNREEGD
                     AVGQSQAFQLDGVNKNVEIKWQIPEIEPQYVLEYQESKQDVQYEIKDLIQIIKKTIER
                     EQRDARFNLTKGRLQKDLINWFIDDQYKLFYKKQDLSKSFDATFTLLIDASASMHDKM
                     AETKKGVVLFHETLKALNIKHEILSFSEDAFDSDDHAQPNIINEIINYDYSTFEKDGP
                     RIMALEPQDDNRDGVAIRVASERLMRRNQHQRFLIVFSDGEPSAFNYSQDGIIDTYEA
                     VEMSRKFGIEVFNVFLSQDPITEDVEQTIHNIYGQYAIFVEGVAHLPGHLSPLLKKLL
                     LKSL"
     gene            2918..4261
                     /gene="brnQ"
                     /locus_tag="P6L01_001880"
     CDS             2918..4261
                     /gene="brnQ"
                     /locus_tag="P6L01_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDP3030937.1"
                     /translation="MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFV
                     LTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSF
                     EMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
                     KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGI
                     TKTNQIFKQTLTAGLIAAVALIFIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLT
                     TMASTGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPKVSYKAFVLVFILMS
                     FIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSI
                     FSVISKLGWLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE"
     gene            complement(4334..5470)
                     /locus_tag="P6L01_001881"
     CDS             complement(4334..5470)
                     /locus_tag="P6L01_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDP3030938.1"
                     /translation="MTENKSFKESHPLDDFISDKELSNTTIQKEKLTIEQQKQVDTIS
                     KQINPLDNEGLLAFGSDLQKQMSQFSHQMLDEVQSKDVGPIGDTLSDLMSKLKSVNPN
                     ELNTDKPSMLKRIFSRAKSSINEIFSRMQSVSAQVDRITIQLQKHQTHLTRDIELLDT
                     LYDKNKQYFDDLSLHIIAAQQKKLQLENEKLPQLQQQAQQSTNQMDIQQVADMQQFID
                     RLDKRIYDLQLSRQIALQTAPQIRMIQNVNQALAEKIQSSILTSIPLWKNQMAIALTL
                     MRQRNAVAAQRAVTDTTNDLLTANAEMLKQNAIETATENERGIVDLDTLKRTQRNIIE
                     TIEETLIIQQHGREERQLAEKELQQLEQDLKSHLVNIKGPNKQS"
     gene            complement(5502..6131)
                     /locus_tag="P6L01_001882"
     CDS             complement(5502..6131)
                     /locus_tag="P6L01_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDP3030939.1"
                     /translation="MTVRYNISHIFGVLVGIPVAFLTSIFGMIALDVSFLIDMSIGIV
                     GFLMTYLPIQKLTSRKYLNEIGLTRKDYRYIRNQLNHTHQKLRGILKTYVNIRSIKDF
                     RQINDIYQISRSIYTTVRQRPASFYKVEGFFYSHIDNALNLVDAYTRLAKMPKKSINE
                     QQKLEQTRITLDEVKRTLIADLKRLNEDDYERLDIEMELNKLHQKHHQD"
     gene            complement(6150..6419)
                     /locus_tag="P6L01_001883"
     CDS             complement(6150..6419)
                     /locus_tag="P6L01_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDP3030940.1"
                     /translation="MRHIHLQVFGRVQGVGFRYFTQRIAMNYNIVGTVQNVDDYVEIY
                     AQGDDADIERFIQGVIEGASPASNVTSHQLEELELNQKLSDFRSI"
     gene            6582..6890
                     /locus_tag="P6L01_001884"
     CDS             6582..6890
                     /locus_tag="P6L01_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDP3030941.1"
                     /translation="MWTVTKIRADYEGWWLFSDWPENIVEKYQYQDFDDMFKHYQQLI
                     NQCKVQFDNYVTGKYNIYAFYNNCDMNYCEDCEEDLQIFYSFIVLQNNEVYYKLPIID
                     "
     gene            7061..7261
                     /gene="cspA"
                     /locus_tag="P6L01_001885"
     CDS             7061..7261
                     /gene="cspA"
                     /locus_tag="P6L01_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspA"
                     /protein_id="HDP3030942.1"
                     /translation="MKQGTVKWFNAEKGFGFIEVEGENDVFVHFSAINQDGYKSLEEG
                     QAVEFEVVEGDRGPQAANVVKL"
BASE COUNT         2469 a         1078 c         1236 g         2607 t
ORIGIN      
        1 aatggcacta aaacattata agaattcaga ttcaacagtt ttcaatgatg cgaaggcatt
       61 atttgattta aataaaaata ttttacttaa aggtccaaca ggttcaggga aaacaaagtt
      121 ggcagaaaca ttaagtgaag ttgttgatac acccatgcat caagtcaatt gttctgttga
      181 tttagataca gaaagcttat taggctttaa aacaattaaa acaaatgcgg aaggtcaaca
      241 agaaattgtc tttgtagatg gtccagttat taaagctatg aaagaggggc atattttata
      301 tattgatgaa ataaatatgg ctaaacctga aacattgcct gtattaaatg gtgtattaga
      361 ttatcgtcgt caaattacga atccatacac tggtgaagta atcaaagctg taccaggatt
      421 taacgttata gcagcgataa atgaaggtta tgttggtact ttgccaatga atgaagcact
      481 aaaaaatcgc tttgttgtta ttcacgttga ttatattgac ggggacattt taaaaaatgt
      541 gattaaagag caaagtttat tacaagatga taaacaaatc gaacaaatta ttaagtttaa
      601 cgaagattta cgtactatgt ctaagcaggg acaaatttct gaagaagccg ctagtatccg
      661 tgcattatta gacttgtgtg atttaatcac tgtaatgcca gttgaacgtg caattaaacg
      721 tacaattatt gataaattgg aagatgaacg tgaacaacaa gcaatatata atgctgtaga
      781 actaaacttt taaagaggga taaaacatga gtgatcgttt cataaaattt aacgacgaac
      841 agttagatgc caagcaagta atgatgttgc aagacttagc tcgactctta ttaaaaaatg
      901 aacaaacaca agttaaaatc caaaaattcc cttactataa tcctgttcaa aatgttttga
      961 ttacgagttg gttttggtca catcgaccaa gccatatcga aatggctggt ttaaaaacag
     1021 atgttatgtt agctgcttac ggttatcata tgatggatgt acaaattgta aatgaagtag
     1081 tacaagataa aacttttaaa caccctaaat tttatcaaca actttttaaa ttattagaag
     1141 atatgcgcgt attaaattct attaaagttg agcgcccaag tactgctaaa ttaattgatc
     1201 ttcgcttaga tacacgggta tcatatacgg aatctcaaat taaagtgtat cgtactaaaa
     1261 cacaatatac agatttatta tttttgtatt tagaacatgc atttttaagt caagatttct
     1321 ttgatatacc atctatacat tcagatttag atgatatttt agtaaatatg ttcttatatt
     1381 tacctaattt tttccaaaat caaaattctg aagataatat gtatctggct caaagaatta
     1441 tgtatcaagt agatgatatt ttaaaagaag atatgctaaa cgaatattat tacttaccta
     1501 aaacactata taatacatta gctagtccag agtttgatga tttaaagaga actgatgcaa
     1561 gtcaagtcga tgggcaagat gatacttcag aagatgatga taacgaaagt gaaaaagctg
     1621 actctaaatc tgcagatagt gagtcaaaag gtggcgccta tttagaaatg gagcttcacg
     1681 aaggacaaaa tagtgaaaca ttaggtaatg atgaagcacg tgaaggcgat gctacagatg
     1741 atatgactga tatgatgacc aaaaagggca aaggatcaaa cgatacgcta aatcgtgaag
     1801 aaggcgatgc tgttggtcaa agccaagcat ttcaacttga tggtgtgaac aaaaatgtag
     1861 aaataaaatg gcaaattcct gaaattgagc cacaatatgt tttagaatat caagaatcga
     1921 aacaagacgt acaatatgaa atcaaagact taattcaaat tataaagaaa acgattgaac
     1981 gagaacaacg agatgcacgt tttaatttaa caaaagggcg attacaaaag gatttaatta
     2041 attggtttat cgacgatcaa tataaattgt tttacaaaaa acaagattta agtaaatcat
     2101 ttgatgcgac gtttacattg ttaattgatg cgtcagcaag tatgcatgat aaaatggctg
     2161 aaacgaagaa aggtgtagtg ttattccacg agacgttaaa agcattgaac attaaacatg
     2221 aaattttatc attcagtgag gatgcctttg attcagacga tcatgcacaa ccaaatatca
     2281 ttaatgaaat tattaactat gattactcaa cctttgaaaa agatggccca cgtattatgg
     2341 cacttgaacc tcaagatgat aatcgtgatg gtgttgctat tagagttgca agtgaacgat
     2401 taatgcgtcg aaatcaacat caacgatttt taatcgtatt ttcagacggc gaaccgtctg
     2461 catttaatta tagtcaagat ggtattattg atacgtatga agctgtagaa atgtcacgta
     2521 aatttggtat tgaagtattt aatgtatttt tgagtcaaga tccaataact gaagatgttg
     2581 aacaaacaat tcataatatt tatggtcaat atgctatttt cgttgaaggt gttgcgcatt
     2641 tacctggaca tctctctcca ttattaaaaa aattactact taaatcttta taaataagtt
     2701 taaaatatga gtttatatga aattgtgaat aaaagtcatc agttactatt taaaaagtga
     2761 atttgaagaa ttttattttc tggcataatt tgatttaaac aacacatgtg ttttgtttct
     2821 gctatgtaca acatatttca tatagactct ttattatttt ttgtgctaaa atatttactt
     2881 gtaaattatt tcgtgtttta aaaaaaggag taactctatg aataaaaata catgggtcat
     2941 tgggtttacg ctcttcgcta tgttttttgg cgcaggcaat ctaatttttc ctcctaatct
     3001 cggattggat agtggtcaat ttttctggcc cgccatttta gcgtttgttc taactgggat
     3061 tggtttacca ttattaggtg tgattgtagg tgcacttgat aaagaaggat atattggcgc
     3121 attaaataaa atttcaccta aattttcaat attgttctta atcatcattt atttgactat
     3181 aggaccactt ttcgcaatac ctagaactgc atctacatct tttgaaatga caattacacc
     3241 aattatacat agcaatagta gtatcgcttt atttatattt acgattatct acttcatagt
     3301 cgttttgtat atttgtttaa atccatctaa gttaatcgat cgtattggtt cattattaac
     3361 accattatta ttgattacta ttttagcgat gattattaaa ggatacttag actttagcgg
     3421 taatagtgct ggaaagggca atgaagcact atatcattct aatttttcaa gttttgctga
     3481 aggctttaca caaggctatt taacaatgga tgccattgca gcaattgctt tttcaatgat
     3541 tgttgttaat gcagtaaaac taacaggcat tactaaaaca aatcaaatat tcaaacaaac
     3601 tttgactgct ggtttaattg cagccgtagc tttaattttc atatatattt cattaggtta
     3661 tattggtaat catatgccag taagtgacat gacgttagat caattgaaat ccaaagatcg
     3721 aaacattggg acatatttat taacgacaat ggcttcaaca ggatttggtt cattcggaaa
     3781 atatttattg ggcatcattg tggcgctggc atgtctaact acagcatgcg ggcttattgt
     3841 tgcagtttct gaatatttcc atagaatcgt acctaaagta tcatacaaag catttgtatt
     3901 agttttcatt ttaatgagtt ttattattgc taaccaaggt ttaaatgctg ttatctcaat
     3961 gtcaattccg gtattaagca ttgtataccc agtagcaata actgttgtat tattaatttt
     4021 aattgccaaa ttcataccga caaaacgcat ttcacaacaa attccagtta ttatcgtatt
     4081 tatattgtcg attttcagtg ttattagtaa gttaggttgg ctgaaaatta actttataga
     4141 atcattgcct ctaagagcgt attctttaga gtggttccca gtagcaatta ttgcaacgat
     4201 attaggctat ctagtcggca tatttgtaaa acaagatcca attaaatatc aacaggaata
     4261 acgaataata ttaaaggaag catctccaat ttaatcgtag gatgcttcct ttttgttata
     4321 tctaattcgt aatttatgat tgtttattcg gtcctttgat gttcactaaa tgtgacttta
     4381 aatcttgttc taattgttgt aattcttttt cagctaattg tcgttcttcg cgaccgtgtt
     4441 gttgaataat taatgtttct tcaattgtct caataatgtt acgctgtgta cgtttcaatg
     4501 tatcaagatc aacaatgcca cgctcatttt ctgttgcagt ttcaatcgca ttttgtttca
     4561 acatttcagc atttgctgtt aataaatcat tagttgtatc agtgacagct cgttgtgcag
     4621 caactgcatt acgctgtctc attaatgtaa gcgcaatggc catttgattt ttccatagtg
     4681 gaatacttgt caaaattgaa ctttgtatct tctcggcaag tgcttgatta acattttgaa
     4741 tcatacgaat ttgtggcgca gtttgcaaag ctatttgtct tgaaagctgt aagtcatata
     4801 tgcgtttatc tagtctatct ataaattgct gcatatctgc aacttgttga atatccattt
     4861 gattagtgga ttgctgcgct tgctgttgca attgtggtag cttttcattt tctaattgca
     4921 actttttttg ctgtgcagca atgatatgca atgataagtc atcaaagtat tgtttgtttt
     4981 tatcatatag cgtatctaat aattcaatat ctcttgttaa atgtgtttga tgtttctgca
     5041 gttgaatcgt tatgcgatcg acttgagcac taactgattg cattcttgaa aagatttcat
     5101 tgatagacga ctttgctctg ctaaaaattc tttttaacat agatggttta tcagtattta
     5161 actcatttgg attaactgac tttagttttg acattagatc tgacaaagta tctccaatag
     5221 gaccaacatc tttactttgt acttcatcca acatttgatg tgaaaattga gacatttgtt
     5281 tctgtaaatc agaaccaaac gctaataaac cttcattgtc taaagggtta atttgtttac
     5341 tgattgtgtc tacctgtttt tgttgttcaa ttgttaactt ttctttttga atagtagtat
     5401 tcgataattc tttatcgctt ataaaatcat ctagtggatg gctttctttg aaacttttat
     5461 tttcagtcat caatcatctc tccaatagtt gctattttac atcaatcttg atgatgtttt
     5521 tgatgtaact tatttaattc catttcaata tctaaacgtt cataatcatc ttcgttgaga
     5581 cgctttaaat cagcgattaa tgttcgtttg acctcatcca aagtaattcg tgtttgttct
     5641 aacttttgct gttcattaat tgattttttg ggcatttttg ctagacgtgt atatgcatca
     5701 accaaattta aagcattatc aatatgagaa taaaaaaagc cttcaacttt ataaaatgat
     5761 gcaggtctct gtctaactgt cgtataaata gaacgtgaaa tttggtatat atcattaatc
     5821 tgcctaaaat ctttaattga tcttatattg acatacgttt ttaaaatacc tctaagtttt
     5881 tggtgtgtat gatttaactg atttcgaata tagcgatagt cttttctagt caaaccaatt
     5941 tcgtttaaat atttgcgtga agtgagtttt tgtatcggta ggtatgtcat taaaaagcca
     6001 acaataccaa tagacatatc aattaaaaaa gatacatcaa gtgcaatcat cccaaatatg
     6061 cttgttaaaa acgctacagg aattcccact aacaccccaa atatatgaga aatattatat
     6121 ctcactgtca tcttccttta tttagcattt tatattgatc gaaaatccga taatttttga
     6181 tttagttcta actcttcaag ttgatggctt gttacatttg atgctggtga ggcaccttca
     6241 attacacctt gaataaatct ctctatatct gcgtcatccc cttgtgcata tatctctaca
     6301 tagtcatcta cattttgaac agtaccgaca atgttatagt tcattgcaat gcgttgtgta
     6361 aaatatctaa atccgacgcc ttgaacgcgt ccgaatactt gtaaatgtat atgtctcatt
     6421 tttaccacct cataatgtta ttatacgtag ttttacttaa aaaaactaat aattactata
     6481 gttactactt tgtttgtttc aagtcgtcaa acttgatttt cagaggataa aggtataaaa
     6541 ataagtatag agtttttgaa gtatggaagg ggtctttaat aatgtggaca gttaccaaaa
     6601 ttagagccga ttatgaggga tggtggttat tcagtgactg gccagaaaac attgttgaaa
     6661 aatatcaata tcaagatttt gatgacatgt ttaagcacta tcaacaattg attaatcaat
     6721 gtaaagttca gttcgataat tatgtcacag gcaaatataa tatttatgca ttttataata
     6781 attgtgacat gaactattgc gaagattgtg aagaggattt acaaatattt tatagtttca
     6841 ttgttttaca aaataatgaa gtatattata aactaccaat aattgattaa atgaatattt
     6901 tttattattg tattgcaatt tggtaataac tgcactataa tacaagttaa gcagatgatt
     6961 attccatatt gcaaagaata tttgagtgaa atttgcatgt attttttgta atatgcgaat
     7021 aagcatattg aatgaatttt agtcttggag gtttcagatt atgaaacaag gtacagttaa
     7081 atggtttaac gctgaaaaag gattcggctt tatcgaagtt gaaggagaaa atgacgtatt
     7141 cgtacatttt tcagcaatta accaagatgg ttacaaatca ttagaagaag gtcaagctgt
     7201 tgagtttgaa gtagttgaag gcgaccgcgg tccacaagct gcaaacgttg ttaaactata
     7261 attcttagat ttgaatcatt gattttaact aacaccttac caagaagtaa ggtgttttta
     7321 tatgcttatt acaaatactt aacgcgatga gtttctcagc caaattgaga actctttttt
     7381 tatatcacca
//
LOCUS       DAOBBH010000122         7386 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.122, whole genome shotgun sequence.
ACCESSION   DAOBBH010000122 DAOBBH010000000
VERSION     DAOBBH010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7386
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.122"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            107..860
                     /locus_tag="P6L01_001886"
                     /pseudo
     CDS             107..860
                     /locus_tag="P6L01_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503564.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
     gene            complement(1062..1331)
                     /locus_tag="P6L01_001887"
     CDS             complement(1062..1331)
                     /locus_tag="P6L01_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030943.1"
                     /translation="MAENNQNSLVTKIATYGSFIAIASFVILFISIFLKFSFNIEFIA
                     TIMNICRYTLILGFILMSLPDVVDKNIKKIIFDAFIIIVFIFFLL"
     gene            1645..2331
                     /gene="mtnN"
                     /locus_tag="P6L01_001888"
     CDS             1645..2331
                     /gene="mtnN"
                     /locus_tag="P6L01_001888"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDP3030944.1"
                     /translation="MIGIIGAMEEEVTILKNKLTQLSEISVAHVKFYTGILKDREVVI
                     TQSGIGKVNAAISTTLLINKFKPDVIINTGSAGALDESLNVGDVLISDDVKYHDADAT
                     AFGYEYGQIPQMPVAFQSSKPLIEKVSQVVQQQQLTAKVGLIVSGDSFIGSVEQRQKI
                     KKAFPNAMAVEMEATAIAQTCYQFNVPFVVVRAVSDLANGEAEMSFEAFLEKAAVSSS
                     QTVEALVSQL"
     gene            2351..2878
                     /locus_tag="P6L01_001889"
     CDS             2351..2878
                     /locus_tag="P6L01_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDP3030945.1"
                     /translation="MGLVRKFFMPNSYVQSIFQIDLDKLVDKGVKGIITDLDNTLVGW
                     DVKEPTERVKAWFKEANEKGITITIVSNNNESRVASFSQHLDIDFIFKARKPMGKAFD
                     KAITKMNIRPDQTVVIGDQMLTDVFGGNRRGLYTIMVVPVKRTDGFITKVNRLIERRL
                     LRHFSKKGYITWEEN"
     gene            2879..3979
                     /gene="yqeH"
                     /locus_tag="P6L01_001890"
     CDS             2879..3979
                     /gene="yqeH"
                     /locus_tag="P6L01_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDP3030946.1"
                     /translation="MSDILKCIGCGAPLQSEDKNKPGFVPEHNMFRDDVICRRCFRLK
                     NYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILA
                     ANKLDLLPKQINKRRVKEWLKRTARKYGLEADDVVLISAEKGWGIDDLLSSIANIREN
                     EDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDESSFMYDTP
                     GIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLV
                     CFFSNDLNIHRTKTEKANDLWRNQLGDLLTPPGNPQNFDLNELKAVRLETGKEKRDVM
                     ISGLGFITIGPGAKVIVRVPKNVDVVLRNSIL"
     gene            3993..4799
                     /gene="aroE"
                     /locus_tag="P6L01_001891"
     CDS             3993..4799
                     /gene="aroE"
                     /locus_tag="P6L01_001891"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDP3030947.1"
                     /translation="MKFAVIGNPISHSLSPVMHRANFNSLGLDDTYEALNIPIEDFHL
                     IKEIISKKELDGFNITIPHKERIIPYLDYVDEQAINAGAVNTVLIKDGKWIGYNTDGI
                     GYVKGLHSVYPDLENAYILILGAGGASKGIAYELAKFVKPKLTVANRTMARFESWNLN
                     INQISLADAEKYLAEFDIVINTTPAGMAGNNESIINLKHLSPNTLMSDIVYIPYKTPI
                     LEEAERKGNHIYNGLDMFVYQGAESFKIWTNKDADINSMKTAVLQQLKGE"
     gene            4803..5093
                     /gene="yhbY"
                     /locus_tag="P6L01_001892"
     CDS             4803..5093
                     /gene="yhbY"
                     /locus_tag="P6L01_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDP3030948.1"
                     /translation="MLTGKQKRYLRSLAHNIDPIFQIGKGGINENMIKQIDDTLENRE
                     LIKVHVLQNNFDDKKELAETLSEATRSELVQVIGSMIVIYRESKENKEIELP"
     gene            5096..5665
                     /gene="nadD"
                     /locus_tag="P6L01_001893"
     CDS             5096..5665
                     /gene="nadD"
                     /locus_tag="P6L01_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HDP3030949.1"
                     /translation="MKKIVLYGGQFNPIHTAHMIVASEVFHELQPDEFYFLPSFMSPL
                     KKHNNFIDVQHRLTMIQMIIDELGFGDICDDEIKRGGQSYTYDTIKAFKEQHKDSELY
                     FVIGTDQYNQLEKWYQIEYLKEMVTFVVVNRDKNSQNVENAMIAIQIPRVDISSTMIR
                     QRVSEGKSIQVLVPKSVENYIKGEGLYEH"
     gene            5655..6239
                     /gene="yqeK"
                     /locus_tag="P6L01_001894"
     CDS             5655..6239
                     /gene="yqeK"
                     /locus_tag="P6L01_001894"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDP3030950.1"
                     /translation="MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVE
                     LAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEV
                     LMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISK
                     RTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD"
     gene            6240..6593
                     /gene="rsfS"
                     /locus_tag="P6L01_001895"
     CDS             6240..6593
                     /gene="rsfS"
                     /locus_tag="P6L01_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDP3030951.1"
                     /translation="MNSQELLAIAVDAIDNKKGEDTISLEMKGISDMTDYFVVTHGNN
                     ERQVQAIARAVKEVANEQNIEVKRMEGYNEARWILIDLADVVVHVFHKDERNYYNIEK
                     LYQDAPLESYGQVAY"
     gene            6596..7312
                     /locus_tag="P6L01_001896"
     CDS             6596..7312
                     /locus_tag="P6L01_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDP3030952.1"
                     /translation="MSQYAEMSLVYDQLTQDQPYEKWFEIVKNHCKDESNILDIGCGT
                     GSLTVQLEALGNVTGMDLSVDMLTVAAQKSANVNWLEGDMTSFDLQQQFDIITIFCDS
                     LNYLQDETAVIETFINVYHHLTDSGVFIFDVHTVYKMMTLFNNQSYIDDKGDIFLAWD
                     AVQGDLPLSVYHDMTFFIRHEDETYSRFDESHFQRTFDEKTYLSWLAQVGFKHVETFT
                     DFNIDEHNEDTERLFFIAKK"
BASE COUNT         2744 a          911 c         1395 g         2336 t
ORIGIN      
        1 tcagtcaact actgccaata taacttcgta gagcatagaa cattgattta tgtcccagcc
       61 tgtttatcat ttatgtctcg ctctcaaatg taaattaata attgttttgg gggagtttga
      121 agttaaatat ttaacaggat ttattttaat attattgtta gaaggaattt ttacaaattc
      181 agcgagtgca atcgaatatt cagacttaca tcataaaagt aagtttgatt caaagcgtct
      241 aagtaatgct aagatgtcat tcatcaatcc aactcagctt gaaaataaaa acacaaacga
      301 tagactgttg aagcatgatt tgttatttca tgacatgttc gtaaatgttg cttcgaaaaa
      361 ggattttaaa gttgaattcg aaaatgaggc actttcaaag aaatttataa ataagaatat
      421 cgatatatat gctggaagct atagctatga atgtcatggg ggagcaacca ataaaacgca
      481 atgtagttat ggtggtgtta ctttaagtga caataataaa taagatgatt ataagaatat
      541 accttctaat ttatggattg acggacatca aacagaaata gaactaactg cagtaaaaac
      601 gaaaaaagaa aattgttacc attcaagaat tagatgttca attaagaaat tatttgaatg
      661 agaagtataa gttgtacgaa caaggtggcg acattgttaa agggtatgtt aaatatcata
      721 atgatgatga acaaaatgta gaatatgatt tttataattt aaatggtgag tatggttatg
      781 aggtattaaa aatgtatgct gataataaaa ctatcaatag agacaaattg catttagata
      841 tctatttatt caaatcataa tttaattaag ttaatcatta ataatctttg acagtgaaat
      901 ataaagtacc tgacattata ttgttacaaa tgaaaacagc agtaaggatg ctaacactta
      961 gaaaagtctt actgctattt tctattagaa ttatctttaa tgcacacagg aattcgatac
     1021 gaaacaaata tgattaactg tgtaattaca aaataaaact actatagtaa aaagaagata
     1081 aagacgatga taataaatgc atcgaatata attttcttga tgtttttatc aacaacatca
     1141 ggtaaggaca taagtataaa tcctagaatt agagtgtagc gacatatgtt cataattgtt
     1201 gctataaact cgatattaaa tgaaaacttt aaaaatatac ttataaataa aatgacaaac
     1261 gacgcaattg caataaaact gccgtatgta gctatctttg ttacgagact attttgatta
     1321 ttttcagcca ttgtaaaatc ctcttatttc atcattatta ttttaaacat atcattttcc
     1381 tgttatttaa taaagtttct tgacacgatt ggttgatgta attgatatga ttagtttttg
     1441 aaaaatagac aaattagaag catcgaattc actgttatga gttcaaaaat aattatttat
     1501 gataccaagt tattgaaaga gctttttata aaaataaagc aaaagtttta tacagtaatg
     1561 aaaatcatga tacttgtcgt tgtagtgatt aaaatgtgat aaaataacaa atgctaggat
     1621 tttttaaaag aggtgaatca aaggatgatt ggtataattg gtgccatgga agaagaagta
     1681 acaatattaa aaaataaatt aacacaatta agcgaaattt cagttgcaca tgttaaattt
     1741 tatactggca ttttaaaaga tagagaagta gtgattaccc aaagtggcat tggaaaagtt
     1801 aatgctgcaa tttctacgac attattaatt aataagttta aaccggacgt cattattaat
     1861 acaggttctg ctggagcttt agatgaaagt ttaaatgtag gtgacgttct tataagtgat
     1921 gatgtaaaat atcatgatgc ggatgcaaca gcatttggtt atgaatatgg acaaatacca
     1981 cagatgccgg tagcatttca atcaagtaaa cctttaatag aaaaagtatc tcaagttgta
     2041 caacaacaac aattaacagc taaagtaggc ttaattgtaa gtggtgatag ctttatcggt
     2101 agtgttgaac aacgccaaaa aattaaaaaa gcatttccaa atgcgatggc ggttgaaatg
     2161 gaagcaactg caattgcaca aacatgttat caatttaatg taccatttgt tgtagttcgt
     2221 gcagtttcag acttagcaaa tggagaagcg gaaatgagct tcgaagcatt tttagaaaaa
     2281 gcagctgtat catcaagtca aactgttgaa gcattagtgt ctcaattata aaaaggtaaa
     2341 ggtgagttaa atgggtttag ttcgcaagtt ttttatgccg aattcatatg ttcaatcaat
     2401 atttcaaatt gatttagaca agttagtgga caaaggcgtt aaaggtatta ttacagattt
     2461 agataatacg ctagtaggtt gggatgttaa agaacctaca gaacgtgtta aagcatggtt
     2521 taaggaagct aatgaaaaag gaatcactat tacaatcgtg tctaataata atgagtctcg
     2581 tgttgctagt tttagtcagc atttagacat cgattttatt tttaaagcga gaaagccaat
     2641 ggggaaagcg tttgataaag caataactaa gatgaatatc agaccagatc aaactgttgt
     2701 tataggtgac caaatgctta ctgatgtatt tggtggtaat cgtcgaggtc tatatacaat
     2761 tatggttgtt ccagttaaac gaactgatgg ctttattact aaggttaata gattaattga
     2821 aagacgatta ttacgtcatt tcagtaaaaa aggttatatc acatgggagg aaaattgatt
     2881 gtctgacatt ttaaaatgta tcggttgtgg tgcgccactt caatctgaag ataaaaataa
     2941 acctggtttt gtaccagagc ataatatgtt tcgtgatgac gtgatttgca gacgttgttt
     3001 ccgcttgaaa aattataacg aagttcaaga tgtaggatta gaaagtgaag actttttaaa
     3061 attattatca ggacttgcgg ataaaaaggg tattgtcgtc aatgtcgtgg atgtatttga
     3121 ctttgaagga tcatttatta atgcagttaa acgtattgtc ggaaacaaaa aaatcatttt
     3181 agcagcaaat aaattggatt tattgccgaa acaaattaat aaacgtcgag ttaaagaatg
     3241 gttaaaacga acagcaagaa aatatggttt ggaagctgac gatgtcgtat taatttcagc
     3301 tgaaaaaggc tggggcatag acgacttatt atcatcaatt gcgaatattc gagaaaatga
     3361 agatgtgtat attgtaggga caacgaatgt tgggaaatct acattgatta ataaactgat
     3421 tgaagctagt gttggtgaaa aagatgtagt aacaacttca agattccctg gaacaacttt
     3481 agatatgata gatattcctt tagatgaatc atcatttatg tatgatacac caggtattat
     3541 tcaagatcat caaatgacgc atttagttag tgaaaaagaa ttgaaaatta ttatgcctaa
     3601 gaaagaaata aaacaacgcg tatatcaatt aaatgaggcg cagacattat tcttcggcgg
     3661 tctagcgcgc atagattatg tatcaggtgg taaacgtccg ttagtttgtt tcttttctaa
     3721 tgacttgaat atacatcgta ctaaaacgga gaaggctaat gatttatggc gtaatcaact
     3781 tggcgattta ttaacgccac ctggaaatcc acaaaatttt gatcttaatg agttaaaggc
     3841 tgttagactt gaaacaggca aagagaaacg tgatgttatg atctctggtc taggctttat
     3901 aactatagga ccaggagcta aagtaatcgt tcgtgttcct aaaaatgttg atgttgtatt
     3961 aagaaattct attttataag gtgatttaaa aaatgaaatt tgcagttatc ggaaatccta
     4021 tttcacattc cttgtcgccc gttatgcata gagcaaattt taattcttta ggattagatg
     4081 atacttatga agctttaaat attccaattg aagattttca tttaattaaa gaaattattt
     4141 cgaaaaaaga attagatggc tttaatatca caattcctca taaagaacgt atcataccgt
     4201 atttagatta tgttgatgaa caagcgatta atgcaggtgc agttaacact gttttgataa
     4261 aagatggcaa gtggataggg tataatacag atggtattgg ttatgttaaa ggattgcaca
     4321 gcgtttatcc agatttagaa aatgcataca ttttaatttt gggcgcaggt ggtgcaagta
     4381 aaggtattgc ttatgaatta gcaaaatttg taaagcccaa attaactgtt gcgaatagaa
     4441 cgatggctcg ttttgaatct tggaatttaa atataaacca aatttcattg gcagatgctg
     4501 aaaagtattt agctgaattc gatatcgtta ttaatacaac accagcgggt atggctggaa
     4561 ataacgaaag tattattaat ttaaaacatc tttctcccaa tactttaatg agtgatattg
     4621 tttatatacc gtataaaaca cctattttag aggaagcaga gcgcaaggga aaccatattt
     4681 ataatggctt agatatgttt gtttaccaag gtgcggaaag ctttaaaatt tggactaata
     4741 aagatgctga tattaattct atgaaaacag cagttttaca acaattaaaa ggagaataac
     4801 atatgcttac tggcaaacaa aaaagatact taagaagttt agcacacaat attgatccga
     4861 tttttcaaat tggaaaaggc ggtatcaacg aaaatatgat taaacaaata gatgatacgt
     4921 tagaaaacag agaattgatt aaagtacatg tactacaaaa taactttgat gataaaaaag
     4981 aattagctga aacattaagc gaagctacgc gtagtgaatt agtgcaagtg attggatcta
     5041 tgatagtgat ttatagagaa tctaaagaaa ataaagaaat tgaattgcca taataatgaa
     5101 aaagatagta ctttacggcg gtcagtttaa ccctatccat actgcacata tgatagtagc
     5161 tagcgaagta tttcatgaat tacagccaga tgaattttat tttttaccta gttttatgtc
     5221 tccattgaaa aagcacaata attttataga tgttcagcac agattaacaa tgatacagat
     5281 gattatcgac gagcttggtt ttggagatat ttgcgacgat gaaattaaac gtggtggtca
     5341 gagttatacc tatgacacga tcaaggcatt caaggagcaa cacaaagaca gtgagttgta
     5401 ctttgttatt gggacggatc agtataacca actagagaaa tggtatcaaa ttgaatactt
     5461 aaaagaaatg gttacttttg tagttgtaaa tcgagacaaa aatagtcaaa atgttgaaaa
     5521 tgctatgatt gcaattcaga tacctagggt agatataagt tcgacaatga ttcgacaaag
     5581 agttagtgaa gggaaatcta tccaagttct tgttcctaaa tccgttgaaa actatattaa
     5641 gggggaagga ttatatgaac attgaaaaag caaaacggct tgcaaaagag aaactgccag
     5701 agaaacgtta taatcattct ttaagagttg cagaaacagc gattaaattg gcagaaattt
     5761 atgatggaga cactagtaaa gtagaattag caggtgtatt acatgatttc tgtaaatatg
     5821 atgatttagg taaaatgtat caaattgttc gacaatacga attaggtaat gatctactga
     5881 gttatggtag tgaaatattg catggccctg tgtgtgcagc aatcatggaa catgaatatg
     5941 gtatcaatga tgaggaagta ttaatggcta tcaaatacca tactactgga cgtcaacaaa
     6001 tgacaaaaac tgaaaaactg atttttattg ctgattacat cgaacctgga agaacaatcc
     6061 caggagttga tgatattcga gatatggcat acaatcaagg tagtttagat aagacaattt
     6121 atgaaatttc taaacgcaca gtactatttt taatacagaa agatattacg gtatataata
     6181 agacgattga ctgtttaaat tattataact atagtgacga aagaataaag gatgattaaa
     6241 tgaattcaca agaattatta gcaattgctg tggatgcaat tgacaataaa aaaggcgaag
     6301 atacgatttc tttagaaatg aaaggtatca gcgatatgac agattatttt gttgtaacgc
     6361 acggaaataa tgaacgacaa gttcaagcga ttgctagagc ggtgaaagaa gtagccaatg
     6421 aacaaaatat agaagtaaaa cgtatggaag gatacaatga agcgcgttgg atattaattg
     6481 acttagctga tgttgtggta catgttttcc ataaagacga aagaaattat tataatattg
     6541 aaaagttata tcaagatgca ccattagaat catatggtca ggttgcgtat taattatgtc
     6601 gcaatatgca gaaatgagcc tagtgtacga tcaattgact caagatcaac catatgaaaa
     6661 atggtttgaa attgtaaaaa atcactgcaa agatgaatca aatattttag atattggatg
     6721 cggtactggt agtttaacag ttcaattaga agctttaggt aatgttactg gtatggattt
     6781 aagtgttgat atgttgactg ttgcagctca aaaatcagca aatgtaaatt ggcttgaagg
     6841 agatatgaca tcttttgatt tgcaacaaca atttgatatc atcactattt tttgtgactc
     6901 tctaaattat ttgcaagatg aaactgcagt gattgaaaca ttcatcaatg tttatcatca
     6961 tctgactgat tcaggtgtat ttatttttga tgtacatact gtttataaaa tgatgacttt
     7021 gtttaataat caaagttata ttgatgataa aggggacatt tttttagcat gggatgctgt
     7081 gcaaggagat ttacctttaa gtgtttatca tgatatgaca tttttcattc gacatgaaga
     7141 tgaaacatat tcaagatttg atgagtctca ttttcagaga acatttgatg aaaagacata
     7201 tttgtcttgg ttagctcaag tcggttttaa acatgttgaa acatttactg attttaatat
     7261 agatgaacat aatgaagata cagaaagatt gtttttcatt gcgaaaaaat aaaagtaaaa
     7321 ccactcgctc agacgtgtat aaagttgagg agtggtttta ttgtatcaat ttttattacg
     7381 ctataa
//
LOCUS       DAOBBH010000123         7312 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.123, whole genome shotgun sequence.
ACCESSION   DAOBBH010000123 DAOBBH010000000
VERSION     DAOBBH010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7312
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.123"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(312..1973)
                     /locus_tag="P6L01_001897"
     CDS             complement(312..1973)
                     /locus_tag="P6L01_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDP3030953.1"
                     /translation="MSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFT
                     SNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFI
                     IGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASL
                     DGFKQLMLDMSSNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLG
                     DNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDVWHL
                     KPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHA
                     PGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYL
                     VRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGM
                     DKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTT
                     KGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI"
     gene            complement(2298..4073)
                     /locus_tag="P6L01_001898"
     CDS             complement(2298..4073)
                     /locus_tag="P6L01_001898"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDP3030954.1"
                     /translation="MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRD
                     GQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEI
                     DNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKTAIKEILDLCLTNEED
                     LDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHIGGLADFSAL
                     KFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVEDIKVDVTTSQKIAREILIHRHGKAE
                     SIKLTVDDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDK
                     FCQNIPKKSWFVSATSTTNNKEIIDTIESICKRDPLAGKTVTGGIITINDSAWQMSFT
                     INRQQQFKDQPENEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVSTDKI
                     EDLAKHVSNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLSFIGNFAETERDTVFTT
                     EYSVRTAMEAVYQLLNIDRGIPEVINSTFDLRVLMDAVYELNDHQDLREITKDSKMQK
                     LALAGFLKKIKGTYIETLLKEHKLL"
     gene            complement(4247..5119)
                     /locus_tag="P6L01_001899"
     CDS             complement(4247..5119)
                     /locus_tag="P6L01_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDP3030955.1"
                     /translation="MNKLRDTTFLSYLFTIILWGSAFPMIKIALNDFSAESLSAFRLI
                     LATIILLPFVIIKKLPTPELRDIPVIFILGFCGFVIYHTALNFGETLISAGISGILVS
                     TTPIFSSALAYIFLKEHFSKWNWLSSLVAFIGISIISISKDDYTTINVLGVFIILLAS
                     FSESLYFTFQKKYIEKYGFIAFTLYTIMASSPFMLIFIPEIINDIHGATFTSIVSVLY
                     LAIFPTIIPYVLLAYIVKSVGVSDATMSLYLTPIVSLLLSYLLLDELPTTLAIIGGII
                     TLLGVSLSNFFQNT"
     gene            5232..6629
                     /locus_tag="P6L01_001900"
                     /pseudo
     CDS             5232..6629
                     /locus_tag="P6L01_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            6819..7292
                     /locus_tag="P6L01_001901"
     CDS             6819..7292
                     /locus_tag="P6L01_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HDP3030956.1"
                     /translation="MSKIRSFTILSLLIYLAMMCYTVVTYSKLPTKVPIHYNLAGDAD
                     NFADKWVLLLINSAFIVIWLIFFIAGRYYERFAKWSHYNHTPREIRAIKLFLSTLNLE
                     IMSYMSIFTVLEIWQIQHHHQLNLLWFNMIFIIIIGLTLVIFCLLPTIHKMRDSQ"
BASE COUNT         2499 a         1166 c         1211 g         2436 t
ORIGIN      
        1 tttattgcgt tgttctaaaa atttggacat ttatatagaa ggaactatat ataatgtgaa
       61 ggttatggca attcgcttca atgaaggcga cgtgaaagtg aatactaaat gattcgaggt
      121 cagactccga gaacagtgtc cgtcatgctt accgcatact aatgaagcat tattatatca
      181 gtgtttatag ccgatttaag ataataaagt ttgctaaaca aaaaagccaa tccattgatg
      241 ttggattgac cttgtatatg cctctgaatc tctaatttta aaaaatatga atataaataa
      301 gacattaaaa attaaatttc agttgttgca atttcttcat ctgtaggtac atcatcgtta
      361 agaccaacaa gtgcttcaga aacatttcgt gaatgataac cgatacgttc aagaacacca
      421 atcatatcga tatatagtaa tccgcctttt gttgtacatt cgccacgatt aaggcgttta
      481 atatgacctt tgcgtagttt atgttcaata ttaaatgatt ctctactacg ttctacaatt
      541 tcatcttttt tcgttttgtc ataaacatct aacatgtcga tggctttatc aaatgactca
      601 gcaacatggt tgaataattt atccataccg cgttgtgcat cttctgtaat gcgaatatct
      661 tcatcatgtt ggcgttttaa ttgagcgaca tactcttctg ttagctctgc tacttttaaa
      721 atagagcgat tgacatcaaa cataactgct aaacgctcaa cgtctgcctt cgtaatggct
      781 tttgtagaaa ttctaactaa ataatttcga atgctatcat tgattgtttc aacagcttgg
      841 tgcttttgtt caagcttctt gatcaatttt ttatcgtctt ttgtaatttc gcgaatgtct
      901 tcaaacattg ataagacaat ctgacccaca ttttgtaatt ctttttgagt ttcttgtaat
      961 gcaacaccag gtgcgtgata aacaagatct ttgtttaagt gctgaggttt atagtcatca
     1021 gcaatatctt tacctgggac aagctttgta actatccatg ctaaacctgc tacaaatggt
     1081 aattgaatca aagtatttgt tatgttgaag ataccatgtg atactgcaat cgtcatcgct
     1141 ggttttaagt gccatacatc ttgtaacaaa ctaatcaaat gaatcacaac tggcaagaaa
     1201 attgtgaaga taattacccc gattaaatta aagatgacgt gtacaagcgc cgcacgtttt
     1261 gcagcgattg agccggctaa actagctaag atagctgtaa ttgttgtacc aatgttatca
     1321 cctagtaaca cagggattgc tgcgtttaag ctgattaaat cttgttgata aaattcttgt
     1381 aaaataccaa tcgtcgcact tgaactttga actagtgctg ttaaccctgc gccgacaatg
     1441 acagcaagta ttggatttga tgacatatca agcattaatt gcttaaatcc atctaatgat
     1501 gctaaaggtt taacggcatc acccataaat tctagaccga agaatagaga accgaaaccg
     1561 aatagtatgc ggccaatgtt attgatttta gagcgtttaa agaaaaagat taaaaatgca
     1621 cctaatgcta aaattggcat tgcatattcg cctaaatcta taccgataat aaatgcagtt
     1681 accgttgttc cgatattggc acccattatc actccaatag cttgttttaa cgtcataaat
     1741 ccagctgtta ccagtccgat tgtgataacg gtcgtacctg aactactttg tattaaaata
     1801 gttacaacga tacctgcaat aacacctaat actggatttg atgtaaattt gtttaaaata
     1861 tctcgtagcc tgtctcctgc tgatgcttga agcccgtctc ccatgatttt taagccgtaa
     1921 aggaaaatac ctaaaccacc taaaaaggag aaaatgactt ctgtaaccga catttccatt
     1981 atttcacctc aaataagctt tatatttaga ttatcgctta taattgtaaa tttaatgtta
     2041 agattaggta aaattattta acaatatatg ttatttgtat atgacttgta aaatatcgtc
     2101 acttattatg taaattttca gtgtgaaatg gcaggtttgc aataacttgt ttaacaaaat
     2161 gatgcaatca atcatgtaat tatgtttcat caaaaaaatc atgtgagtgg gataacgaaa
     2221 taaagtttgt gaacatatca tttctatccc actccatgat ttgaaatcac caaataaaaa
     2281 tctattaatg gttttcgtta taacaatttg tgttctttta ataatgtctc aatgtacgta
     2341 ccttttatct ttttaaggaa tcctgctaat gcgagtttct gcattttcga atctttagta
     2401 atctcgcgca aatcttgatg gtcattcagt tcgtatacgg catccattaa gacgcgaaga
     2461 tcaaatgtac tattgatgac ctctggaata ccacgatcta tatttagtaa ttgataaaca
     2521 gcttccatgg cagtacgaac cgaatattct gttgtaaata cagtatctcg ctctgtttct
     2581 gcaaagttac caataaatga taaattttgt gattgatgcg ggacgactaa aggtctgtcg
     2641 ccgatagcac gcgtcatgaa ataagaggtg atatatggca tataaacagg aatcgtatta
     2701 gatacatgtt ttgctaagtc ttcaattttg tcagttgata cacctaagtg atacagccat
     2761 tcttggcata tttcattacc actacattct gtaattggct ttttaatata atcgccgttt
     2821 acatctgaat ataaggcata aatccatgta gatatttcat tttcaggttg gtctttaaac
     2881 tgttgctgac gattgattgt aaaactcatt tgccatgcag aatcattgat tgtaataata
     2941 ccgcctgtaa ctgttttgcc tgcaagtggg tcacgtttac aaatactttc tattgtatcg
     3001 ataatctctt tattgtttgt tgtagaagtt gctgaaacaa accaactttt tttaggaata
     3061 ttttggcaaa acttatcagg attaccaaat tcaggacttt gtcgcgctaa atttttccat
     3121 agtgtccaac taccacctaa ttcgtcagtt ggtggtgctg gtgtatcatt atcaccatac
     3181 gtagagcttt ccgtaatact accgtttgtc acaaagacaa gatcatctac agtcagttta
     3241 attgactctg ctttgccatg tcggtgaatt aatatttctc gggcaatttt ttgacttgtc
     3301 gtaacatcta ctttaatatc ttctactttt acatcgtatt caaattgaac cccatgcgat
     3361 tttaaatatt caaccatagg taatactaaa gattcatatt gattatattt agtgaatttt
     3421 aaagctgaaa agtctgcgag accaccaata tgatgaacga atcgcattag ataacgacgc
     3481 atttccatag cagaatgcca cggttcaaat gcaaacatcg ttttccagta aatccaaaag
     3541 tttgaattaa agaagtcatc ggaaaataca tctgttattt tgacatcatc taaatcttct
     3601 tcattcgtta agcataaatc taaaatttct ttaatcgccg ttttagtcaa agtgaagtct
     3661 ccgtctgtga ctaaacgttg accctgtttc tcaataacac gacagcgaga atagttaggg
     3721 tcttctttgt ttagccaata gaactcatct aatacagacg cgttatcgat ttctaatgaa
     3781 gggatagatc tgaataagtc ccacaaacat tcaaagtggt tctccatttc acgaccaccg
     3841 cggacaacat agccttttaa aggcatattt tcaccatcaa gactaccacc tgctttaggt
     3901 aactcttcta aaatatgaat cttcgaacct tccatttgac catccctaat taagaaacaa
     3961 gctgccgcaa gtgaagctaa cccagacccg attaagtaag cagatttgtt ttctacattt
     4021 tcaggttttt tagggcgcgc aaatgcttca taatttccat aactgtaata catatccacc
     4081 aagtcctttc gtattagaat acactcagac tatacccctt tgtgaattgt aaatattagt
     4141 atttatattg gtaaatttga cgaaattaga tcatttttat atttttgatt tttaaaattt
     4201 tatcactcga atgtttttgg attctgaggt gcgggacttg gaataattat gtattttgaa
     4261 agaagttact taaactaacg cctagtagag tgataattcc gcctataata gcaagggttg
     4321 ttggtagctc gtctaataac agataagata ataataaaga aacgataggt gttaaataaa
     4381 gagacattgt tgcatcagag acaccaactg acttcacaat ataagcaagc aaaacgtatg
     4441 gaattatagt agggaatata gctaaataaa gtaccgatac tattgatgta aaagtggcgc
     4501 cgtgtatatc gttgatgatt tcaggaataa aaataagcat aaatggtgag cttgccatta
     4561 ttgtatatag tgtgaaagcg atgaagccgt atttttctat gtattttttc tggaaagtaa
     4621 aatacaaact ttcactaaaa gatgcaagta aaataataaa aacacctaat acattaatag
     4681 ttgtgtaatc atctttactt attgaaataa tggatattcc tataaatgcg acgagtgaac
     4741 ttaaccaatt ccattttgaa aaatgctctt ttaaaaatat ataagctaaa gcactagaaa
     4801 aaataggcgt tgtagagact agaattccag atatacctgc actaatcaaa gtttcaccaa
     4861 aatttaaagc tgtgtgatat atcacaaatc cacaaaatcc taaaataaaa ataacaggga
     4921 tatctcttag ttcaggggta ggcaatttct ttataattac gaacggcaag agaattattg
     4981 ttgctaaaat taaacgaaat gccgacaatg attctgcact aaaatcattt aacgcaatct
     5041 ttatcattgg aaatgcagat ccccacaata tgatagtaaa taaatatgat agaaaagtag
     5101 tgtctcgaag tttattcatt aatatcatca ctcctttaat tatgtgtttc tatattaaaa
     5161 aatatgattt aaaatgagta caaccaattt tgaatggatt tacctatcca attttaaaag
     5221 ggagggagaa gatggctaaa tattaagata ttgctagtga cataagagat aaaataatca
     5281 caggggattg gttttatgga atgaagatac ctccacagag gcagttggcg atacaataca
     5341 acgtaaatag agtgacgatt attaaaagta ttgagttatt agaggctgaa ggatttatct
     5401 atactaaagt ggggagtgga acatatgtta atgactattt gaatgaagca catattacaa
     5461 ataagtggtc tgaaatgatg ttatggtcct ctcaacaaag aagtcagtat acggtgcaat
     5521 taattaataa aattgagaca gatgattcgt atatacatat aagtaaaggt gaattgggta
     5581 tatcgttaat gccacatatt caattgaaaa aagccatgtc taatacagcc agtcatattg
     5641 aagacttatc ttttggttat aataatggct atggttatat caagttaaga gatattatcg
     5701 ttgaacgaat gtcaaagcaa ggtataaatg taggtagaga aaatgtaatg atcacttcag
     5761 gcgctttaca tgccattcaa cttttatcta ttgggttttt aggtcaagat gccataataa
     5821 tttcgaatac accatcatat attcactcta caaatgtttt tgagcaattg aattttagac
     5881 atattgatgt tccttataat caaattaatg aaattaatac catcattgat agatttatta
     5941 attttaaaaa taaagcgatt tatatagaac ctaggtttaa taacccgaca ggtcgttctt
     6001 taacgaatga gcaaaagaaa aatataatta cttatagcga aagacataat attcctatca
     6061 ttgaagatga tatctttaga gatattttct ttagcgatcc aactcctgct atcaaaactt
     6121 atgataaatt gggaaaagtt atacatataa gcagtttttc aaaaacgatt gcaccagcaa
     6181 taagaatagg ttggattgtt gcttctgaaa aaataataga gcaattggca gatgtaagaa
     6241 tgcaaattga ctatggatcc agtattttgt cacaaatggt tgtatatgag atgttgaaaa
     6301 ataagtctta tgataaacac ttagtaaagt taaggtatgt tttaaaagat aaacgagact
     6361 ttatgttaaa catcctcaat aatttattta aggatatagc acattgggag gttccaagtg
     6421 gaggttattt tgtatggtta gtctttaaaa tagatataga tattaaatat ttattttacg
     6481 aattgttaag taaagaaaaa atattaatca atccgggtta catttatggc agtaaagaaa
     6541 agagtataag gctatctttt gcctttgaat caaatgaaaa tattaagcat gcgctctata
     6601 aaatttatac atatgtgaaa aaggtttaat taaaacaata attcgaatca ttatgtggca
     6661 tgttaaacag ctaaatataa gctatgcaca tttaacaagc ggatactcct gacgcaatta
     6721 tagtatcatt acatattgtt taatcgtttg gggtaatgta attaaaaggt aaaataggca
     6781 gagtgttata cataaaatgt aaagaaagag gcgatagaat gtctaaaatc aggtctttta
     6841 caatattaag tctacttatt tacttagcta tgatgtgcta tacagtagtg acctattcaa
     6901 aattaccaac caaagtacct attcactata atttagcagg ggatgctgat aactttgctg
     6961 ataaatgggt actgcttttg ataaatagcg catttatagt gatttggctt atatttttca
     7021 ttgcaggtag atactatgaa cgatttgcca aatggtcaca ttataatcat acaccacgtg
     7081 aaattcgagc gattaaatta tttttaagta cgttaaattt agagattatg agctatatgt
     7141 ctatcttcac agtattagaa atttggcaaa tacaacacca tcatcaatta aatttactat
     7201 ggtttaatat gatatttatc attatcattg gtttaacgct tgtcatattt tgtttacttc
     7261 ctacaattca caaaatgaga gattctcaat aaaaatataa actgtcatga tg
//
LOCUS       DAOBBH010000124         7261 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.124, whole genome shotgun sequence.
ACCESSION   DAOBBH010000124 DAOBBH010000000
VERSION     DAOBBH010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7261
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.124"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            49..837
                     /locus_tag="P6L01_001902"
     CDS             49..837
                     /locus_tag="P6L01_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDP3030957.1"
                     /translation="MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLA
                     SSYKVLTYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLF
                     TDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKSEAYILLFSKLFRNQEK
                     TMKWYQKQRIYSIKSEDDSAVAIRSLILHKDEPMYLKKRTCVPTLLINGEHDPLIKDK
                     NHFKLEAHFLNVTKKIFEHSGHAPHIEEPEAFMNYYLNFLKSVS"
     gene            complement(1088..1462)
                     /locus_tag="P6L01_001903"
     CDS             complement(1088..1462)
                     /locus_tag="P6L01_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HDP3030958.1"
                     /translation="MAITKINDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYIS
                     ENKEKEYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRK
                     KIESLLSRVNKRITEANNEIEL"
     gene            complement(2410..3339)
                     /locus_tag="P6L01_001904"
     CDS             complement(2410..3339)
                     /locus_tag="P6L01_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDP3030959.1"
                     /translation="MLLFYIIGVLVGMLIPIQTSVNSRLSLYTKSPFYTSFISFSVGT
                     ICSIILNIIINPEVFTIHFYNNQSFNYTWVVGGLLGVSFLTGNLLLLPKLGATLTVIA
                     TVAGQIIMGVIIDTFGLFGATIHDFNLIKAIGVLLLIVGIVIMNQFNKNNLLLTDQKY
                     LLFWLLLGFIFGFFPPIQTTINSALASHTHSPAFASLVSFTIGSITLLILTAIFNSSL
                     KLKTSHLKFGKLKPIYFTGGILGMAFVTANIILMPHMGAALTTLIGMFGQILMGILID
                     HFGLFGSPKIAMTSRKTIGLLCILTGIILLRLF"
     gene            complement(3534..3758)
                     /locus_tag="P6L01_001905"
     CDS             complement(3534..3758)
                     /locus_tag="P6L01_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HDP3030960.1"
                     /translation="MTKTLELSFKSSLDKPVKLQLPQLNNVVTEQVARDSMNALVDLN
                     IIKSNSGTITKVHSAQIIDKTTTVLFEDKK"
     gene            complement(3775..3978)
                     /locus_tag="P6L01_001906"
     CDS             complement(3775..3978)
                     /locus_tag="P6L01_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030961.1"
                     /translation="MSKINHITIVLSFTKVDANGKQTEFKRRFANINPDASNDQIKTF
                     SKLIERLTGETYNNIELIKSLSI"
     gene            4137..4697
                     /locus_tag="P6L01_001907"
     CDS             4137..4697
                     /locus_tag="P6L01_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDP3030962.1"
                     /translation="MNKVEAIKFNDDIVKMYEALKIKSERDYLFFKLAIHSGLKVSEL
                     LTITVSQVKRLIEKCTLSEMCKAHFHSLIKIRLPETLSKELLQYIEDRSLSNEDVLFQ
                     SLRTNQVLSRQQAYRIIHQASIEAGIDNVGLTTLRKTFAYHAYQKGIPIPVIQKYLGH
                     QSAIETLNFIGLENECEHSIYISLQL"
     gene            4716..7118
                     /gene="mnhA2"
                     /locus_tag="P6L01_001908"
     CDS             4716..7118
                     /gene="mnhA2"
                     /locus_tag="P6L01_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit A"
                     /protein_id="HDP3030963.1"
                     /translation="MSLVYLLIAILVIMAMILLMSKRRALAKYAGYIALVAPVISSIY
                     FLIQIPSVAKLQYLSTSIPWIKTLDINLDLRLDGLSLMFSLIISLIGIAVFFYATQYL
                     SSRKDNLPRFYFYLTLFMFSMIGIVLSDNTILMYIFWELTSVSSFLLISYWYNNGDSQ
                     FGAIQSFMITVFGGLALLVGFIMLYIMTGTNNITDILGQADHIKNHGLFIPMIFMFLL
                     GAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLRFTPLLGLSNMYIYI
                     VTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGYAQHQQDAIASI
                     YVFVLFGALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMRQLFPKMNLVMTIA
                     ALSMAGVPFLNGFLSKEMFLDALTQTGQLSQFSLISMIAIVFVGVIASIFTFTYALYM
                     VKEVFWTKYDSKVFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVPALRGV
                     SGGNHQIDPLAPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSK
                     GYEMVYRHFEKFATKRFKRVMQDRLNQYIIMTLGIFMIIIGYGYIRIGLPKVHQLHVS
                     EFGALEIILAIVTVTIGISLIFIRQRLTMVILNGVIGFVVTLFFIAMKAPDLALTQLV
                     VETITTILFIVSFSRLPNVPRSNANKKREIIKISVSLLMALIVVSLIFITQQTDGLSS
                     ISDFYLKADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDE
                     RE"
     gene            7105..>7261
                     /gene="mnhB2"
                     /locus_tag="P6L01_001909"
     CDS             7105..>7261
                     /gene="mnhB2"
                     /locus_tag="P6L01_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit B"
                     /protein_id="HDP3030964.1"
                     /translation="MKENDVVLRTVTKLVVFILLTFGFYVFFAGHNNPGGGFIGGLIF
                     SSAFILMF"
BASE COUNT         2518 a          993 c         1164 g         2586 t
ORIGIN      
        1 tatatagaat gaaggttggg tatatagttt attgagggag gtgtcacaat gaataaagtc
       61 acaattaatc ctcaaatcca attaacttat caaattgaag gtaaagggga tcctataata
      121 ttacttcatg gattggatgg taatttagct ggatttgaag atttgcaaca tcaactagca
      181 tcatcatata aagtacttac ttacgattta agaggtcatg gcaagtcttc taaaagtgaa
      241 tcatacgatt taaacgatca cgttgaggat ttaaaaattc taatggagaa gttaaatatt
      301 catgaggcac atattctagg acatgattta ggtggggtag ttgctaagtt atttacagat
      361 aaatatgctt atcgtgtaaa atcattaact accattgcat cgaagaaaga tgacttaata
      421 cacagcttta ctcaattgtt aatacaatat caagatgata tagcgggttt taataagtct
      481 gaagcgtata ttcttttatt ttctaaattg tttagaaatc aagagaagac gatgaaatgg
      541 tatcaaaaac aaagaatata tagcattaag tctgaggatg atagtgcggt ggcaattcgt
      601 tcattaattt tgcataaaga tgaacctatg tatttaaaaa aacgtacatg tgtacctact
      661 ttgttaatta atggggaaca tgatcctttg attaaagata agaatcattt taaattggaa
      721 gcgcattttt taaatgttac gaaaaaaatc ttcgaacatt caggacatgc accgcatatt
      781 gaagaaccag aagcatttat gaattattat ttaaattttt taaaaagcgt atcataatat
      841 gtgatatata aacctagggc ataaagtcct taggcaatgt gaaaaagctg attactattc
      901 attatttgat agaaatcagc ttttttgaaa tgtatttgat atatactgct cgttatgcgg
      961 ctatcttcct tatattaagt gccattagtg caaaacctct taacaattag gtaaaaaaag
     1021 acataaaaaa aggaagttta atagaatgta tcatctatca aacttcacca aattgcgcta
     1081 aacaaaatta tagttcaatt tcgttgtttg cttcagtgat tcgtttattt actcgactca
     1141 ataatgattc gattttttta cgttgttgtg cattaacaag aattaataca gttctttcat
     1201 catgctcatt acgtttttta tcgaagtaat cttcttgaga taaaatttta actgctttaa
     1261 caacttgtgg ttgtttgtag tttaaatgat taataatatc tttaagatag tattctttct
     1321 ctttgttttc gctgatgtat gtcaatacag cgaattcttc aaagctaatt gaaaattcct
     1381 ttttaattaa actttttaat ttgtcagcat aagtgaccat tgataacaac tcaaagcaat
     1441 cattgatttt tgtaattgcc atgtttaaaa cctccctatt tgatgcatct tgctcgatac
     1501 atttgcccga taatatattg atatctaatc tttatttatt atagatatgt tagtcataat
     1561 tttgcattaa ataagtttta ttaaatatat ttaatgctct attatttagt taattataac
     1621 taattaaaaa tgagaagtaa acaaaaaagt gtttataaaa caaattattc attagacaca
     1681 gtgattgtat ttctgggtta gcatttggtt tagtcaaaaa tatcagcatt ttttataatt
     1741 taccttaatt taatcgccaa tacaaggtaa tttatttaat gaaacgattt agcgcaatta
     1801 aatgtttcga tattcaatgt gtgtctaaag agattctttg ttatagctta actcttccaa
     1861 gttaacttct tctactattt atacccattt ttcagaattt tatcactaaa aatgaaaaat
     1921 ttttataatt tttttaattt agtaattctc agtgcagttt taatgataag aaaaacacat
     1981 taatttagtg attaaaacca atctatagca cgttaaagtg tatatacgtt attccgattg
     2041 ataacgggta acaaataata gaaagtacga taattgatca tgcattaaag cacttttatt
     2101 tttcaaactc gactcaatga attataaaaa gttgtgcatt ttgacttatt tttaattaat
     2161 tgaataacca actatgaaat attagcacga ataacataat ttttgctatt aatttggtgt
     2221 aaattattaa aaaataacat taatttaggt cttaagaatg agttgactat cgaatttgct
     2281 aaattaaaat ttgttgtgtc aaagtatact acccaaatca acgcttttct aaagattttt
     2341 agaccacaaa aaaatagctt atcactttta aaaataatta caagttaaaa tgataagcta
     2401 aaagttaatt taaaataatc ttaaaagtat aatgcctgtc aaaatacata atagaccaat
     2461 agtttttctg gatgtcattg ctattttagg tgaaccaaat aatccaaagt gatctatcaa
     2521 tatgcccatt agaatctggc caaacatccc aataagtgtt gttaatgctg cacccatatg
     2581 aggcattaag ataatgttag ctgttacaaa agccatacca agtataccgc cagtaaaata
     2641 gataggcttt aatttaccga attttaaatg acttgttttt agttttaaag aactattaaa
     2701 aatagcggtt aaaatcaata gcgttattga cccaattgta aatgatacta atgatgcaaa
     2761 ggctggtgaa tgagtatgac ttgctaaagc actattaatt gtcgtttgaa taggtggaaa
     2821 gaaaccaaaa ataaatccta agagaagcca aaacagtaaa tacttttgat cagttagtaa
     2881 taaattattc ttgttaaatt gattcattat gacgatgccg acaatgagta acaatactcc
     2941 aattgcttta attaaattaa aatcatgaat tgtagcgcca aataagccaa atgtatcaat
     3001 aatgacaccc ataataattt gacccgcaac tgttgcaatt acagttaatg ttgcacctaa
     3061 ttttggcaat aacaataaat tgccagttaa aaagctaacc ccaagcaaac caccgactac
     3121 ccatgtgtag ttaaatgatt gattattgta aaagtgaata gtaaatactt ctggattgat
     3181 aatgatattt aaaataattg aacaaattgt tccaactgaa aatgaaatga atgaagtata
     3241 gaaaggcgat ttagtataca gtgatagtct tgaattgaca gatgtttgga taggaataag
     3301 cattccaact aggacaccaa tgatatagaa aagaagcata tgaataaagt ctcactttcc
     3361 aaagttaata ataaaatcat atgaatacat tatcattata gagaaaatat acaactcatt
     3421 tacaataatg ttgaaaaaaa caggacatta tcataacaaa atcaccctag acaatcatga
     3481 tgaaagtcta gggtgttagt atttatacta aattaattgc gctttcttta tatctatttc
     3541 ttatcttcaa ataaaacagt tgttgtttta tcaataattt gagcagaatg tactttggtg
     3601 atagtaccac tatttgactt tataatattt aaatctacta atgcattcat actatcacgt
     3661 gcaacttgct cggtcactac gttgtttaat tgtggtaatt gcagttttac aggcttgtct
     3721 agtgaagatt taaaacttaa ttcaagtgtt ttagtcatga atatgtcctc ctgattaaat
     3781 tgataaagat ttgatgagtt cgatattgtt atatgtttct ccagtaagtc gctcaataag
     3841 tttgctgaat gttttaattt ggtcgtttga tgcatcaggg ttaatgttag cgaatcgacg
     3901 cttaaattct gtttgtttgc cgttagcgtc tactttagta aatgataata caatagtgat
     3961 gtggtttatt ttactcatat tttaaaacct cctttcacac tatatatcga aataaaataa
     4021 taaaatggct aattttattt tctatgttta aaatctataa aaaaggcaat agatatatgt
     4081 aactaaaata taaaatagct atgattaaga cacttaaagc aaggggaggt tgagtaatga
     4141 ataaagtaga agcgattaaa tttaatgatg atattgttaa aatgtatgaa gcgctcaaga
     4201 taaaatctga acgtgactat ttattcttta agttagctat acatagtgga ttgaaagtat
     4261 cagaattatt aacaattaca gtctctcaag ttaagagact aattgaaaag tgtacgttat
     4321 cagaaatgtg taaagcacat tttcattcgt tgattaaaat taggttacca gaaacattat
     4381 cgaaagaact acttcaatat atagaggaca ggagtctttc gaatgaagac gttctttttc
     4441 aatcactacg aacaaatcaa gtattatcta gacagcaagc atatcgaata attcaccaag
     4501 catcaattga agctggtata gataatgtag gactaacgac attgcgtaag acatttgcat
     4561 atcatgctta tcaaaaaggt atacctatac cagtcattca aaagtattta gggcatcaat
     4621 ctgctattga aacactaaat tttatcggtt tagaaaatga gtgtgaacat agtatttata
     4681 tttcattaca attatagaaa caaaggaggc taataatgag tttggtttat ttattaattg
     4741 ctatacttgt gattatggcg atgatacttc taatgtctaa acgtagagca ttggctaaat
     4801 atgccgggta catagcgttg gttgcacctg taatttcatc tatctatttt ttgattcaaa
     4861 taccatcagt agctaaactg caatatcttt ctacctctat tccatggatt aagacattag
     4921 atattaattt agatttacgt ttagatggtt taagtttaat gttttctctt attatttcac
     4981 ttattggaat tgcagtattc ttctatgcaa ctcaatattt atcctctcga aaagacaatt
     5041 taccaaggtt ttatttttat ttaacgttat ttatgttcag tatgattggt attgtattat
     5101 cagacaatac gatattgatg tacatttttt gggaattaac gagtgtatca tcatttttat
     5161 tgatttcata ttggtataac aatggtgaca gtcaatttgg tgcgattcaa tcatttatga
     5221 ttacagtatt tggtggattg gcgttattag ttggttttat tatgctgtat atcatgacag
     5281 gaacgaataa tatcactgac atattaggac aagcagatca tattaagaat catggattgt
     5341 ttatccctat gatttttatg tttttattag gtgcatttac aaaatcagca caatttccat
     5401 ttcatatttg gctacctaga gcaatggctg cacctacacc tgtaagtgct tatttacatt
     5461 cagccacgat ggtaaaagct ggtatctttt tattacttcg atttacacca ttattaggtc
     5521 ttagcaatat gtacatatat atcgtcacgt ttgttggttt aataacaatg ttatttggtt
     5581 caatcacagc tttaaaacaa tgggatttaa aaggaatcct agcatattca acaatcagtc
     5641 aactaggaat gattatggca atggtcggta tcggtggtgg atatgctcaa caccaacaag
     5701 acgcaatagc atctatttat gtatttgtat tatttggtgc gctatttcat ctaatgaatc
     5761 atgccatctt taaatgtgcg cttttcatgg gagtaggtat tttagatcat gaagcaggtt
     5821 caagggatat acgaatttta agtggaatgc gtcaactatt tcctaaaatg aatctagtca
     5881 tgacgatagc ggctctatct atggctggag taccattttt aaatggattt ttaagtaaag
     5941 aaatgttttt agatgcatta acacaaactg gacaattatc ccaatttagt ttgatttcaa
     6001 tgatagctat cgtgtttgtt ggtgttattg ctagtatttt tacattcaca tatgcacttt
     6061 acatggttaa agaagtattt tggactaaat atgattctaa agtgtttact aaaaaaaata
     6121 tccacgaacc atggttgttt agtttaccct ctcttatatt aatggtgcta gtacctgtaa
     6181 tcttttttgt gccaaatata tttgggaagg ggattatcgt tccagcatta agaggtgtat
     6241 caggtggtaa tcatcaaatt gatccattgg caccacatgt ttcgcaatgg catggattta
     6301 acataccgct tcttttaacc atcatcatta ttttattggg tagtgtacta gcaatcaaag
     6361 tagattggaa aaaagtgttc acaggtaaaa ttagacagat ttcagtttca aaagggtatg
     6421 agatggtata tcgacatttt gaaaagtttg ctacgaagcg atttaaacgt gttatgcaag
     6481 atcgtttaaa ccaatacatt attatgacct taggcatatt tatgattatc attggatatg
     6541 gttatattcg aattggactt cctaaagtac atcagttaca tgtttctgaa tttggggcat
     6601 tagaaattat attagcaatc gtaactgtca caattggtat ttctttaatt tttatacgtc
     6661 aacgactgac aatggtcatt ttaaatggag tcatcggatt tgttgtgacc ttattcttta
     6721 tagcaatgaa agcccctgat ttagcattga ctcagctagt agttgaaaca ataacgacga
     6781 tactatttat tgtcagtttt tcaagattac caaacgtacc aagatctaac gctaacaaaa
     6841 aaagagaaat aattaaaatt tctgtatcac tcttgatggc acttattgtt gtatcattaa
     6901 tttttattac acaacaaaca gatggtttat catcaatatc agacttttat ttaaaagctg
     6961 acaaactaac aggtggtaaa aatattgtaa atgcgatact tggtgacttt agagcattag
     7021 atacattatt tgaaggatta gtgttaatta ttactgggct aggtatttac acattattaa
     7081 attatcaaga tcggagggga caagatgaaa gagaatgatg tcgtgttaag aacggtcacg
     7141 aaacttgttg tatttatttt attgactttc ggattctatg tcttcttcgc aggtcataat
     7201 aatcctggtg gtggctttat tggtggttta atatttagtt cagcgtttat tttaatgttt
     7261 c
//
LOCUS       DAOBBH010000125         7221 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.125, whole genome shotgun sequence.
ACCESSION   DAOBBH010000125 DAOBBH010000000
VERSION     DAOBBH010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7221
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.125"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..623)
                     /locus_tag="P6L01_001910"
     CDS             complement(<1..623)
                     /locus_tag="P6L01_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDP3030965.1"
                     /translation="MVVLLSYSCSCIFSFLYQFISIEETSFDYLHRRSKCDYCNSSLK
                     WYELMPIISFLLLKGRCRNCRKRISLTHFLGETFALIPIVFIKYDFTYVNATLFITTY
                     VFLLIFTMTDITSLMLDCRLIIIYCIVSLSLSMIYPVAFIIISMTTHIFYFLFRAYIG
                     YGDVLLISALSLFFPLQFTIYVILFTFVIAGLVALITMIFKPIKLLPL"
     gene            complement(893..2164)
                     /locus_tag="P6L01_001911"
     CDS             complement(893..2164)
                     /locus_tag="P6L01_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDP3030966.1"
                     /translation="MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHV
                     GGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRI
                     KPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPV
                     LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVSVIYAVKNEDALKYVRERAIEQHA
                     KPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGL
                     IELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVD
                     ILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDEIQLVDDYVE
                     FIKNYQGDTLVITGSLYFISEVKSKLDFEAL"
     gene            complement(2177..4807)
                     /locus_tag="P6L01_001912"
     CDS             complement(2177..4807)
                     /locus_tag="P6L01_001912"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDP3030967.1"
                     /translation="MEMKPKYDPREVEAGRYEEWVKNGYFKPSEDKSKETYTIVIPPP
                     NVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGIT
                     RYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVD
                     LYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTR
                     PETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPA
                     HDPNDFEIGQRHQLENIIVMDENGKMNDKAGKYEGMDRFDCRKQLVKDLKEQDLVIKI
                     EDHVHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFN
                     QWMENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSS
                     ALWPFSTLGWPDLESEDFKRYYPTNALVTGYDIIFFWVARMIFQGLEFTDRRPFNDVL
                     LHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVES
                     VWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDK
                     YEFGEVGRALYNFIWDDFCDWYIEMSKIPMNSNDEEQKQVTRSVLSYTLDNIMRMLHP
                     FMPFVTEKIWQSLPHEGDTIVKASWPEVRESLIFEESKQTMQQLVEIIKSVRQSRVEV
                     NTPLSKEIPILIQAKDKEIETTLSQNKDYLIKFCNPSTLNISTDVEIPEKAMTSVVIA
                     GKVVLPLEGLIDMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRK
                     KQDYQEKYDGVKARIEQLKA"
     misc_binding    complement(4851..5152)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5210..5770
                     /locus_tag="P6L01_001913"
     CDS             5210..5770
                     /locus_tag="P6L01_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDP3030968.1"
                     /translation="MNECAFGTKDPVYLNYHDHVWGQPLYDSKALFKLLALESQHAGL
                     SWLTILKKKEAYEEAFYDFEPEKVAQMTAQDIDRLMTFPNIVHHRKKLEAIVNQAQGY
                     LKIEQAYGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPV
                     TVFSFLEAAGLYDAHLKDCPSKPKHN"
     gene            complement(5993..7066)
                     /locus_tag="P6L01_001914"
     CDS             complement(5993..7066)
                     /locus_tag="P6L01_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDP3030969.1"
                     /translation="MTMIYRNNFIVFVLSFFISIILYSSHVLLPFMFGPIIASIICVK
                     VFKLDIKWPFLLSELGIVLLGVQIGSTFTKNVVMDIKNNWLSIIVVSISILLIAIVMA
                     FIFKKIARINTETAILSVIPGALTQMLVMAEQDKRANLLVVSLTQTSRIIFVVVLVPF
                     ISYFFHDGNMHANGKLTKVLPLSQVLNIGQIVILAIAIFIVYLIMSKIKFPTFQLLAP
                     LIVLIVWNFSTGLTFTLDHWLLNMAQLIYMIRIGVQIAHLLSDLKGRLAIAITIQNIM
                     LIIGALIMVYIIHFFDNNPINELFLGAAPGGVNQIVLVALATGADVAMITSYHIFRIF
                     FILFIIAPGINYFLKYRSNKRED"
     gene            complement(7100..7201)
                     /locus_tag="P6L01_001915"
     CDS             complement(7100..7201)
                     /locus_tag="P6L01_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030970.1"
                     /translation="MGGDLIVKVHICFVVKTASGYCYLNKREAQAAI"
BASE COUNT         2439 a         1334 c          990 g         2458 t
ORIGIN      
        1 aggggtaata gtttaatcgg cttaaatatc atggtaatta aagcaactaa accagcaatg
       61 acaaatgtaa ataaaatgac ataaatagtg aattggagag ggaaaaacaa agaaagtgca
      121 gatattagta aaacgtcacc ataaccaata tatgcccgaa ataaaaagta gaatatatgc
      181 gtggtcatac taataatgat aaaagctact ggataaatca tacttaacga gagagaaacg
      241 atacaataaa ttataattaa gcgacaatct aacattaaag aagtgatatc ggtcatagta
      301 aaaataagca gaaaaacata tgtagttata aatagcgtag catttacgta tgtgaaatca
      361 tacttaataa agacgatagg tattaaagca aaggtttccc ctaagaaatg tgttagggaa
      421 atacgctttc gacagtttcg acatcgccct tttaataata aaaaactaat aatcggcatt
      481 aattcatacc atttgagtga cgaattacaa taatcacatt tcgatcttct atgtaaataa
      541 tcaaatgacg tttcttctat agatataaat tgatatagaa aactaaaaat acaactgcag
      601 ctataagata acaatactac caaatgacaa cctccttatg taaattatag ttagttatta
      661 ccaaaatgta aatatacact atttttcaag aattgaaccg ctttttcatt taaatttttc
      721 aatattgcta agcataattg atggatactt taacaaccca ttactgctcg gcaaaattaa
      781 taatggcaag aaattgaacc ttataaacac atacgattta gagcataaaa aataaccatg
      841 aagctctacc tattgattaa atagattctt catggctatt ttagttttag ttttataatg
      901 cttcaaagtc taattttgat ttaacttcac ttatgaaata cagactaccg gtaattacta
      961 atgtatcacc ttgataattt tttataaatt caacgtagtc atctactaat tgtatttcat
     1021 cattttcaat actacctaca atttcttctt tgcgtaacgc tttcggaaaa tcaaattcag
     1081 ttgcataaaa cgtatgcgca attaaactta aatgtttgac catctcgtta atcggttttc
     1141 cgtttattgc tgagaacaaa atatctactt tttctttatc atggtactgt ttaattgtat
     1201 caattagagc atctatactc tctgaattat gtgcgccatc caaaatgatt aaaggtttgt
     1261 catgcacctg ctcaatacgt ccagtccaac gaactgattc aataccgtct atcatcttat
     1321 tgaaatctaa ttcaattaat ccttgttcat ttaattcaat aagagctgtt atggctaatg
     1381 cagcattttg tttctgatgt tcacctaaca tgcttaaaat gattgtttct aattcataat
     1441 ctttataacg gtaagtaaat tcatcatttt gcgatacaac aacaatttct ctatctaatt
     1501 caattggctt tgcatgttgt tcaattgcgc gttcacgaac atattttaat gcatcttcat
     1561 ttttaacagc atatatcact gaaacgttag gctttataat cgcgccttta tccctagcaa
     1621 tatctagata agtaccacct aaaatatctg tatggtctag accgatacta gttaagattg
     1681 ataaaaccgg tgtaaagaca tttgtcgaat cgttctttat acccaatcca gcctcaacaa
     1741 tgacaaaatc aacaggatgt atttcaccaa aatataaaaa catcatcgct gtgattattt
     1801 cgaattcagt tgcaacacct aaatctgttt cacgttccat catttcactt actggtttaa
     1861 tacgtgatac taattctaca atagcgtcat ttgatattgg cacaccattt agactaattc
     1921 gttcattaaa tgtttcaata aacggcgacg taaatgtacc tacttcataa ccattttcaa
     1981 ctaaagctgt tctaaggtaa gcaactgtag agcctttacc atttgtgcca cctacatgaa
     2041 tacccttaat gttattttga ggattattaa attgtgctag catccattcc atacgtttaa
     2101 cacctggttt gatgccaaat ttagttcttt cgtgtatcca atacaagctc tctaggtaat
     2161 tcattgttac taactcctat gcttttaatt gttcaattct tgccttcaca ccatcatatt
     2221 tttcttgata atcttgtttt ttacgttttt cttcatttat aaccttttca ggtgctttac
     2281 ttacaaagtt ttcattagag agctttttat ctactctatc taattcgctt tgaagtttag
     2341 ctaattcttt ttccaaacgg ctgatttcct tatccatatc aattagccct tctaatggta
     2401 ataccacttt acctgcaatt acaactgatg tcattgcttt ctcaggaatt tccacgtcag
     2461 tgctaatatt taaggtacta ggattacaga atttgattaa ataatctttg ttttgtgata
     2521 aagttgtttc aatttcttta tctttagctt gaattaaaat aggtatttct ttagacaatg
     2581 gcgtatttac ttctacacgt gattgtctta cagatttaat gatttcaaca agttgttgca
     2641 ttgtttgttt actttcttca aaaatcaatg attcacgcac ttctggccat gaagctttaa
     2701 caattgtgtc accttcatgt ggtaaacttt gccatatttt ctctgttaca aatggcatga
     2761 atggatgtag cattctcata atattgtcta aagtataact caatactgaa cgtgtaactt
     2821 gtttttgttc ttcatcatta ctattcattg gaattttact catttcaatg taccaatcac
     2881 agaaatcatc ccaaatgaaa ttatataatg cacgtccaac ttcgccgaat tcatatttgt
     2941 cacttaaatc agtaactgtt gcaatcgttt catttaaacg tgttagaatc catttatctg
     3001 ctaatgataa gttaccactt aaatcgatat cttcaacttt aaagtcttca ccgatattca
     3061 ttaaactgaa acgtgcccca ttccagattt tattgataaa gttccacact gactcaactt
     3121 tttcagttga gtatcttaaa tcatgtcctg gagatgaacc tgttgctaag aagtaacgca
     3181 agctatcagc accgtattcg tcaataacat ccattggatc cacaccatta cctaatgatt
     3241 tactcatctt acgcccgtct tcagcacgaa ctaaaccgtg taataataca tcattaaatg
     3301 gacgacgatc tgtaaattct aagccttgga atatcatgcg tgcgacccag aaaaagataa
     3361 tatcgtaacc tgtaactaag gcatttgttg ggtagtatcg tttaaagtct tcactttcta
     3421 aatcaggcca acctaacgta gagaaaggcc ataaagcact tgagaaccac gtatctaata
     3481 catcttcatc ttgttgccaa ttttcaatat cagttggcgc ttcttctcca acatatattt
     3541 cgcctgtttc tttatgatac caagccggaa tttgatgacc ccaccataat tgtcttgaaa
     3601 tcgtccaatc tctaatattt tccatccatt ggttaaatgt atgttcgaaa cgttgcggat
     3661 aaaaatcaat acgatcatct gttttttggt tatctaatga acgtttcgct aagtcttcca
     3721 tgcgcacaaa ccattgtgtt gataaatatg gttcaacaac agcgccagat cgttctgaat
     3781 gacctacaga atgaacatga tcttcaatct tgataactaa atcttgttct tttaaatctt
     3841 taactagctg tttacgacaa tcaaaacggt ccataccttc atatttaccc gctttgtcgt
     3901 tcattttacc attttcatcc ataacgataa tattttctaa ttgatgtctt tgaccaattt
     3961 caaaatcatt agggtcatgt gctggtgtca ctttcatagc accagaaccg aagtctatat
     4021 caacatactc atctgctaaa ataggcagtt cgcgtcctac gattggtaat ataacagttt
     4081 taccgattac atctttgtat cgttcgtcat tagggttaac aacaatcgct gtatcaccta
     4141 acatcgtttc tggtcttgtt gttgcaattt caataaaacc ttcaccatca gcgtaaggat
     4201 atttaaaatg ataaaacgca ccttgaacat cttcatgtat tacttcaata tcagataaag
     4261 ctgtacgtgc tttaggatcc caatttataa tacgttcgcc acgataaata attcctttat
     4321 tgtataaatc aacaaaaact tttttaactg ctttacttaa accttcatct aaagtaaaac
     4381 gttctctact ataatctaaa cctagaccta atttagccca ttgcgcacga ataaatgacg
     4441 catactcttc tttccaatcc catgcctgtt ctaaaaactt ttcacgacca agatcatatc
     4501 tagttattcc ttgttcattt aatttagctt ctacctttgc ctgtgtcgca ataccagcat
     4561 gatccatacc tggtaagtat aacgtatcgt atccttgcat acgtttcata cgtgtaatga
     4621 tatcttgtaa agtcgtatcc catgcatgtc ctaaatgtaa tttaccagtt acatttggtg
     4681 gcgggataac aattgtatat gtttcttttg atttatcttc tgacggttta aaataaccat
     4741 tctttaccca ttcttcataa cgtcccgctt caacttcacg aggatcatat tttggtttca
     4801 tttccatttg ctatacctcc taaaaaaata aaaatatcca tcctatatac aaataggacg
     4861 gatattccgt ggtaccacct atattcaaga aggatgttta atatcaaatt cactctttta
     4921 acataattgg aataatcata ccaatactat catcgtgaaa tttgaaatgc ttcatctctt
     4981 caagcactct agattatgat taacgctcaa acacgtctta gcctactatt aatcacgttc
     5041 agctaagata ctctgtgggc taccttcagt aaaaatcatt tacatactca caccaaatca
     5101 tatgctctct ttaaaataat ttgaacttac tcttcccaaa tcctatatta aactcttaac
     5161 ttatagtata atgattgaca aaataagtca atgtataggt gggaataaaa tgaatgaatg
     5221 cgcatttggt actaaagatc cagtctactt aaactatcat gatcatgtat ggggacaacc
     5281 gctctatgat agcaaggcat tgtttaaact tttagcatta gaatcacaac atgctgggct
     5341 atcttggtta actattttaa aaaagaaaga agcctatgaa gaagcatttt atgatttcga
     5401 accagaaaag gtagcacaaa tgaccgctca agatatcgac cgcttaatga cttttccaaa
     5461 tatcgttcat catcgtaaaa aattagaagc aattgttaat caagctcaag ggtatttaaa
     5521 aattgaacaa gcatatggta gttttagtaa atttttatgg tcttatgtaa atggtaagcc
     5581 taaagatttg cagtatgaac atgcttctga tcgtatcaca gttgatgata ctgcaacaca
     5641 actatctaaa gatttaaaac aatacgggtt taaattttta ggtccagtaa cagtattttc
     5701 gtttttagaa gcagctggtt tatatgatgc acatttaaaa gattgtccat caaagcctaa
     5761 acacaattaa tttgctatag catcataaag taaacgagac atcatctatt tttaactaat
     5821 agactgtctc gttttttgat gtgaaaattt attcaaatca taagttaaag ttccaagtta
     5881 atgcactgct aatattagat atcttaatct aaaaatagat gtacaacctt tgcttaaacc
     5941 ttaataatga attctatcct aagcataatt caacttaaat ggatgatagt ttctaatctt
     6001 cacgcttatt cgaacgatat tttaaaaagt aattaattcc tggtgcaata ataaatagga
     6061 tgaaaaagat tcggaaaata tgataactcg taatcatagc aacatcggca ccagtagcta
     6121 atgcaactaa aactatctga ttaacccctc ctggtgctgc accaagaaac aattcattaa
     6181 taggattatt atcaaagaaa tgtatgatat aaaccatgat tagcgcacca attatcaaca
     6241 taatattttg aattgtaatt gcgattgcta gtctaccttt taaatctgac aataaatgcg
     6301 caatttgaac tccaattcta atcatatata ttagttgtgc catgttcaac aaccaatgat
     6361 ctagtgtaaa tgttaaacct gtagaaaaat tccaaacaat taatacaatg agtggtgcta
     6421 ataattgaaa tgttggaaac tttattttag acataattag ataaactata aagatagcta
     6481 tcgctaaaat aactatttgc cctatgttta atacttgtga taaaggcaag acttttgtta
     6541 actttccatt cgcatgcatg ttaccatcat gaaaaaaata tgaaatgaac ggtactaaaa
     6601 caacaacaaa tataattcgt gatgtttgcg ttaagctaac aactaacaaa ttagcacgtt
     6661 tgtcttgttc agccatgacc agcatttgtg ttagtgctcc tggtataaca cttaaaatag
     6721 ctgtttctgt attaatacgt gcaatttttt taaaaataaa tgccattact attgcaatta
     6781 ataatatcga aatagataca acaataatcg aaagccaatt gtttttaata tccataacga
     6841 catttttcgt aaacgttgat ccgatttgca cccctaatag tacaatccct aattcactaa
     6901 gtaagaatgg ccatttaata tcaagtttga aaactttcac acaaatgatt gatgcgataa
     6961 taggaccaaa cataaatggg agtaatacgt gcgatgaata caatataata ctaataaaaa
     7021 atgataaaac gaacacaatg aaattatttc tatatatcat tgtcatgttt tccacttctt
     7081 tcaataaaaa ataaaatgac taaattgctg cttgagcttc acgtttgtta agataacaat
     7141 atccgctagc agttttgact acaaagcata tatggacttt cactatcaag tcgccgccca
     7201 tgcctgatat acatttaaaa a
//
LOCUS       DAOBBH010000126         7111 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.126, whole genome shotgun sequence.
ACCESSION   DAOBBH010000126 DAOBBH010000000
VERSION     DAOBBH010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7111
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.126"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            138..6953
                     /gene="sasA"
                     /locus_tag="P6L01_001916"
     CDS             138..6953
                     /gene="sasA"
                     /locus_tag="P6L01_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin SasA"
                     /protein_id="HDP3030971.1"
                     /translation="MSKRQKAFHDSLANEKTRVRLYKSGKNWVKSGIKEIEMFKIMGL
                     PFISHSLVSQDNQSISKKMTGYGLKTTAVIGGAFTVNMLHDQQAFAASDAPLTSELNT
                     QSETVGNQNSTTIEASTSTADSTSVTKNSSSVQTSNSDTVSSEKSEKVTSTTNSTSNQ
                     QEKLTSTSESTSSKNTTSSSDTKSVASTSSTEQPINTSTNQSTASNNTSQSTTPSSVN
                     LNKTSTTSTSTAPVKLRTFSRLAMSTFASAATTTAVTANTITVNKDNLKQYMTTSGNA
                     TYDQSTGIVTLTQDAYSQKGAITLGTRIDSNKSFHFSGKVNLGNKYEGHGNGGDGIGF
                     AFSPGVLGETGLNGAAVGIGGLSNAFGFKLDTYHNTSKPNSAAKANADPSNVAGGGAF
                     GAFVTTDSYGVATTYTSSSTADNAAKLNVQPTNNTFQDFDINYNGDTKVMTVKYAGQT
                     WTRNISDWIAKSGTTNFSLSMTASTGGATNLQQVQFGTFEYTESAVTQVRYVDVTTGK
                     DIIPPKTYSGNVDQVVTIDNQQSALTAKGYNYTSVDSSYASTYNDTNKTVKMTNAGQS
                     VTYYFTDVKAPTVTVGNQTIEVGKTMNPVVLTTTDNGTGTVTNTVTGLPSGLSYDSAT
                     NSIIGTPTKIGQSTVTVVSTDQANNKSTTTFTINVVDTTAPTVTPIGDQSSEVYSPIS
                     PIKIATQDNSGNAVTNTVTGLPSGLTFDSTNNTISGTPTNIGTSTISIVSTDASGNKT
                     TTTFKYEVTRNSMSDSVSTSGSTQQSQSVSTSKADSQSASTSTSGSIVVSTSASTSKS
                     TSVSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSGSVSKSTSLSDSISNS
                     NSTEKSESLSTSTSDSLRTSTSLSDSLSMSTSGSLSKSQSLSTSISGSSSTSASLSDS
                     TSNAISTSTSLSESASTSDSISISNSIANSQSASTSKSDSQSTSISLSTSDSKSMSTS
                     ESLSDSTSTSGSVSGSLSIAASQSVSTSTSDSMSTSEIVSDSISTSGSLSASDSKSMS
                     VSSSMSTSQSGSTSESLSDSQSTSDSDSKSLSLSTSQSGSTSTSTSTSASVRTSESQS
                     TSGSMSASQSDSMSISTSFSDSTSDSKSASTASSESISQSASTSTSGSVSTSTSLSTS
                     NSERTSTSVSDSTSLSTSESDSISESTSTSDSISEAISASESTSISLSESNSTSDSES
                     QSASAFLSESLSESTSESTSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSASIS
                     TSTSISESTSTFKSESVSTSLSMSTSTSLSNSTSLSTSLSDSTSDSKSDSLSTSMSTS
                     DSISTSKSDSISTSTSLSGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTS
                     TSLSDSTSTSLSLSASMNQSGVDSNSASQSASNSTSTSTSESDSQSTSTYTSQSTSQS
                     ESTSTSTSLSDSTSISKSTSQSGSTSTSASLSGSESESDSQSISTSASESTSESASTS
                     LSDSTSTSNSGSASTSTSLSNSASASESDSSSTSLSDSTSASMQSSESDSQSTSASLS
                     DSLSTSTSNRMSTIASLSTSVSTSESGSTSESTSESDSTSTSLSDSQSTSRSTSASGS
                     ASTSTSTSDSRSTSASTSTSMRTSTSDSQSMSLSTSTSTSMSDSTSLSDSVSDSTSDS
                     TSASTSGSMSVSISLSDSTSTSTSASEVMSASISDSQSMSESVNDSESVSESNSESDS
                     KSMSGSTSVSDSGSLSVSTSLRKSESVSESSSLSGSQSMSDSVSTSDSSSLSVSTSLR
                     SSESVSESDSLSDSKSTSGSTSTSTSGSLSTSTSLSGSESVSESTSLSDSISMSDSTS
                     TSDSDSLSGSISLSGSTSLSTSDSLSDSKSLSSSQSMSGSESTSTSVSDSQSSSTSNS
                     QFDSMSISASESDSMSTSDSSNISGSNSTSTSLSTSDSMSGSVSVSTSTSLSDSISGS
                     TSVSDSSSTSTSTSLSDSMSQSQSTSTSASGSLSTSISTSMSMSASTSSSQSTSVSTS
                     LSTSDSISDSTSISISGSQSTVESESTSDSTSISDSESLSTSDSDSTSTSTSDSTSGS
                     TSTSISESLSTSGSGSTSVSDSTSMSESDSTSVSMSQDKSDSTSISDSESVSTSTSTS
                     LSTSDSTSTSESLSTSMSGSQSISDSTSTSMSGSTSTSESNSMHPSDSMSMHHTHSTS
                     TSRLSSEATTSTSESQSTLSATSEVTKHNGTPAQSEKRLPDTGDSIKQNGLLGGVMTL
                     LVGLGLMKRKKKKDENDQDDSQA"
BASE COUNT         2587 a         1390 c         1307 g         1827 t
ORIGIN      
        1 catctagacg aataaaaatg aatttttaca aattttaaca aaatgagtat ttttgtatca
       61 aaaaagagtt gtattaaatt gaatagtggc atatattata atctaatgaa tgtaaagata
      121 atacaaggag ttattacatg agtaaaagac agaaagcatt tcatgacagc ttagcaaacg
      181 aaaaaacaag agtaagactt tataaatctg gaaaaaattg ggtaaaatcc ggaattaaag
      241 aaatagaaat gttcaaaatt atggggctac catttattag tcatagttta gtgagtcaag
      301 ataatcaaag cattagtaaa aaaatgacgg gatacggact gaaaactacg gcggttattg
      361 gtggtgcatt cacggtaaat atgttgcatg accagcaagc ttttgcggct tctgatgcac
      421 cattaacttc tgaattaaac acacaaagtg aaacagtagg taatcaaaac tcaacgacaa
      481 tcgaagcatc aacatcaaca gccgattcca caagtgtaac gaaaaatagt agttcggtac
      541 aaacatcaaa tagtgacaca gtctcaagtg aaaagtctga aaaggtcact tcgacaacta
      601 atagtacaag caatcaacaa gagaaattga catctacatc agaatcaaca tcctcaaaga
      661 atactacatc aagttctgat actaaatctg tagcttcaac ttcaagtaca gaacaaccaa
      721 ttaatacatc aacaaatcaa agtactgcat caaataacac ttcacaaagc acaacgccat
      781 cttcggtcaa cttaaacaaa actagcacaa cgtcaactag caccgcacca gtaaaacttc
      841 gaactttcag tcgcttagct atgtcaacat ttgcgtcagc agcgacgaca accgcagtaa
      901 ctgctaatac aattacagtt aataaagata acttaaaaca atatatgaca acgtcaggta
      961 atgctaccta tgatcaaagt accggtattg tgacgttaac acaggatgca tacagccaaa
     1021 aaggtgctat tacattagga acacgtattg actctaataa gagttttcat ttttctggaa
     1081 aagtaaattt aggtaacaaa tatgaagggc atggaaatgg tggagatggt atcggttttg
     1141 ccttttcacc aggtgtatta ggtgaaacag ggttaaacgg tgccgcagta ggtattggtg
     1201 gcttaagtaa cgcatttggc ttcaaattgg atacgtatca caatacatct aaaccaaatt
     1261 cagctgcaaa ggcgaatgct gacccatcta atgtagctgg tggaggtgcg tttggtgcat
     1321 ttgtaacaac agatagttat ggtgttgcaa caacgtatac atcaagttca acagctgata
     1381 atgctgcgaa gttaaatgtt caacctacaa ataacacgtt ccaagatttt gatattaact
     1441 ataatggtga tacaaaggtt atgactgtca aatatgcagg tcaaacatgg acacgtaata
     1501 tttcagattg gattgcgaaa agtggtacga ccaacttttc attatcaatg acagcctcaa
     1561 caggtggcgc gacaaattta caacaagtac aatttggaac attcgaatat acagagtctg
     1621 ctgttacaca agtgagatac gttgatgtaa caacaggtaa agatattatt ccaccaaaaa
     1681 catattcagg aaatgttgat caagtcgtga caatcgataa tcagcaatct gcattgactg
     1741 ctaaaggata taactacacg tccgtcgata gttcatatgc gtcaacttat aatgatacaa
     1801 ataaaactgt aaaaatgacg aatgctggac aatcagtgac atattatttt actgatgtaa
     1861 aagcaccaac tgtaactgta ggcaatcaaa ccatagaagt gggtaaaaca atgaatcctg
     1921 ttgtgttgac tacaacggat aatggtactg ggactgtgac aaatacagtt acaggattac
     1981 caagcggatt aagttacgat agtgcaacga attcaatcat tgggacacca acaaaaattg
     2041 gtcaatcaac agtgacagtt gtgtctactg accaagcaaa taacaaatcg acgacaactt
     2101 ttacaataaa tgttgtggat acgacagcac caacagtgac accaatagga gatcaatcat
     2161 cagaagtgta ttcaccaata tccccgatta aaattgctac gcaagataac agtggaaatg
     2221 cggtgacgaa tacagtgact ggattgccat ccggactaac atttgatagt acaaataata
     2281 ctattagtgg tacaccaaca aacattggta caagtactat atcaatcgtt tctacagatg
     2341 cgagcggtaa caaaacgacg acaactttta aatatgaagt aacaagaaat agcatgagtg
     2401 attccgtatc aacatcagga agtacacaac aatctcaaag tgtctcaaca agtaaagctg
     2461 actcacaaag tgcatcaacg agtacatcag gatcgattgt ggtatctaca tcagctagta
     2521 cctcgaaatc gacaagtgta agcctatctg attctgtgag tgcatctaag tcattaagca
     2581 catctgaaag taatagtgta tcaagctcaa caagcacaag tttagtgaat tcacaaagtg
     2641 tatcatcaag catgtcaggt tcagttagta aatcaacatc attaagcgat tcgatttcga
     2701 actctaacag tactgaaaaa tccgaaagtc tatcaacaag tacatctgat tcattgcgta
     2761 catcaacatc actcagtgac tcattaagta tgagtacatc aggaagcttg tctaagtcac
     2821 aaagcttatc aacgagtata tcagggtcgt ctagtacatc agcatcatta agtgacagta
     2881 catcgaatgc aattagtaca tcaacatcat tgagcgagtc agctagcacc tcggactcta
     2941 tcagtatttc aaatagcata gccaactctc aaagtgcgtc aacaagcaaa tcagattcac
     3001 aaagtacatc aatatcatta agtacaagtg attcaaaatc gatgagtaca tcagaatcat
     3061 tgagcgattc gacgagcaca agtggttctg tttctggatc actaagcata gcagcatcac
     3121 aaagtgtctc aacaagtaca tcagactcga tgagtacttc agagatagta agtgactcta
     3181 tcagtacaag tgggtcatta tctgcatcag acagtaaatc aatgtctgta agtagttcaa
     3241 tgagcacgtc tcagtcaggt agtacatcag aatcattaag tgattcacaa agtacatctg
     3301 attctgatag taagtcatta tcactaagta ctagtcaatc aggttcaaca agtacatcaa
     3361 cgtcgacaag tgcttcagta cgtacttcgg aatcgcaaag tacgtctggt tcaatgagtg
     3421 caagtcaatc cgattcaatg agcatatcaa cgtcgtttag tgattcaacg agtgatagca
     3481 aatcagcatc aactgcatca agtgaatcaa tatcacaaag tgcttctacg agcacatctg
     3541 gttcggtaag tacttcgaca tcgttaagta caagtaattc agaacgtaca tcaacatctg
     3601 tgagtgattc cacaagctta agtacatcag agtctgattc aataagtgaa tcaacgtcaa
     3661 cgagcgattc tataagtgaa gcaatatctg cttcagagag cacgtctata tcattaagtg
     3721 aatcaaatag tactagcgat tcagaatcac aaagtgcatc tgccttttta agtgaatcat
     3781 taagtgaaag tacgtctgaa tcaacatcag agtcagtgag tagttcgaca agtgagagta
     3841 cgtcattatc agacagtaca tcagaatctg gtagcacatc aacatcatta agtaattcaa
     3901 caagtggtag tgcgtccatt tcaacatcga caagtatcag tgaatcaacg tcaacgttta
     3961 agagcgagag tgtttcaaca tcactgagta tgtcaacgag tacaagtttg tctaactcta
     4021 caagtttgtc aacatcatta agtgattcca caagtgatag taagtctgat tcattaagta
     4081 catcaatgtc gacaagtgat tcaatcagta caagtaaatc tgattccatt agtacatcta
     4141 catcattaag tggttctaca agtgaaagtg aatccgactc aacatcatca agtgaaagta
     4201 aatccgattc aacatcaatg agcataagta tgtctcaatc aacatcagga agtacaagta
     4261 cgtcaacgag tacaagtttg tctgactcaa cgagtacgtc attgtcacta agtgcatcaa
     4321 tgaatcaaag cggagtagac tcaaactcag caagccaaag tgcctcaaac tcaacaagta
     4381 caagcacgag cgaatccgat tcacaaagca catcaacata tacaagtcag tcaacaagcc
     4441 aaagtgaatc aacatcgaca tcaacgtcac taagcgattc aacaagtata tctaaaagta
     4501 cgagccaatc aggttcaaca agcacatcag catcattaag tggttcagag agtgaatctg
     4561 attcacaaag tatctcaaca agtgcaagtg aatcaacatc agaaagtgcg tcaacatcac
     4621 tcagtgactc aacaagtaca agtaactcag gatcagcaag tacgtcaaca tcgctcagta
     4681 actcagcaag cgcaagtgaa tccgattcgt cgtcaacatc tttaagtgat tcaacatctg
     4741 cgtcaatgca aagcagtgaa tccgattcac aaagcacatc agcatcatta agtgattcgc
     4801 taagtacatc aacttcaaac cgcatgtcga ccattgcaag tttatcaaca tcggtaagta
     4861 catcagaatc tggctcaaca tcagaaagta caagtgaatc cgattcaaca tcaacatcat
     4921 taagcgattc acaaagcaca tcaagaagta caagtgcatc aggatcagca agtacatcaa
     4981 catcaacaag tgactctcgt agtacatcag cttcaactag tacttcgatg cgtacaagta
     5041 ctagtgattc acaaagtatg tcgctttcaa caagtacatc aacaagtatg agtgattcaa
     5101 cgtcattatc tgatagtgtg agtgattcaa catcagactc aacaagtgcg agtacatctg
     5161 gttcgatgag tgtatctata tcgttaagtg attcgacaag tacatcaaca tcggctagtg
     5221 aagtaatgag cgcaagcata tctgattcac aaagtatgtc agaatctgta aatgattcag
     5281 aaagtgtaag tgaatctaat tctgaaagtg actctaaatc gatgagtggc tcaacaagtg
     5341 tcagtgattc tggctcattg agcgtctcaa cgtcattaag aaaatcagaa agtgtaagcg
     5401 agtcaagttc attgagtggc tcacaatcga tgagcgattc agtaagcaca agcgattcgt
     5461 catcattaag tgtatcgacg tcactaagaa gttcagaaag cgtgagtgaa tctgattcat
     5521 taagtgattc aaaatcaaca agtggttcga cttcaacaag tacatctggt tcattgagta
     5581 cctcaacatc attaagtggt tcagaaagcg taagcgagtc tacctcgcta agtgattcaa
     5641 tatcaatgag tgattctact agtacaagtg actccgactc attaagtgga tcaatatctt
     5701 taagtggttc cacaagtctt agcacttcgg attcattaag tgattcaaaa tcattgagta
     5761 gctcgcaaag tatgagtgga tcagaatcaa cgtcaacaag tgtgagcgat tcgcagtcaa
     5821 gctcaacaag taatagtcaa tttgactcta tgagcatcag tgcatcagaa agcgactcaa
     5881 tgtctacaag tgattcgtct aacatcagtg gatcaaattc aacgagtaca tcactttcaa
     5941 catctgactc aatgagcgga agcgtatcag tttcaacatc gacaagttta agtgactcaa
     6001 tatcaggttc aacaagtgta agtgactcga gctcaacaag cacatctaca tcattaagtg
     6061 attcaatgtc acaaagccag tcaacaagta caagtgcatc tggttcctta agtacatcga
     6121 tatcaacatc aatgtcaatg agtgctagta catcgtcatc acaaagcaca tcggtgtcga
     6181 catcattatc aacatcagac agtatcagtg attctacttc aataagtatc agtggttcac
     6241 aaagtacagt agaatcagaa tctacaagtg attcaacttc tatcagtgac tcagaatcat
     6301 tgagtacatc agattcagac tcgacatcga caagtacatc ggactcaaca agtggttcaa
     6361 cttcaacaag catatctgaa tcattaagta cgtctggttc aggttcaacg agcgtatctg
     6421 actcaacatc aatgagtgaa tccgattcaa caagtgtttc aatgtcacaa gacaaatccg
     6481 actcaacatc aattagcgac tcagaatcag tgtcaacaag cacatcaacg tcattgagca
     6541 catccgattc gacaagcaca tccgaatcgc tgagtacatc tatgtctggt tcacaaagca
     6601 tttctgactc aacatcaaca agtatgtccg gctcaacaag tacatctgaa tctaactcaa
     6661 tgcatccgtc agactcaatg agtatgcatc atactcacag cacgagcaca tctcgcttat
     6721 caagtgaagc aacaacgagc acgagtgaat ctcagtctac attaagtgca acatctgaag
     6781 tgactaaaca taatggcacg cctgcacaaa gtgaaaaaag attgccagat acaggtgact
     6841 caataaaaca aaatggatta ctaggtggcg ttatgacatt attagttggt ttaggtttaa
     6901 tgaagagaaa gaaaaagaaa gatgaaaatg atcaagatga ttctcaagca taaattgaat
     6961 ttatagctgg ctaaaattaa aaagactgag gcaccaatca ctagaaaaat cgatatagga
     7021 ttttaataat tagtgatgaa cccttagtct ttttaatgtt tttgatataa aagaggtgca
     7081 acatgttaaa acttttacaa caatacgaat a
//
LOCUS       DAOBBH010000127         7076 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.127, whole genome shotgun sequence.
ACCESSION   DAOBBH010000127 DAOBBH010000000
VERSION     DAOBBH010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7076
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.127"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            112..702
                     /locus_tag="P6L01_001917"
     CDS             112..702
                     /locus_tag="P6L01_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDP3030972.1"
                     /translation="MKVNPNNIELIISAVKEEQYPETELSEVALSGRSNVGKSTFINS
                     MIGRKNMARTSQQPGKTQTLNFYNIDEQLIFVDVPGYGYAKVSKTQREKFGKMIEEYI
                     TKRENLQLVIQLVDLRHDPTQDDILMYNYLKHFDIPTLVICTKEDKIPKGKVQKHIKN
                     IKTQLDMDPDDTIVSYSSIQNNKQQQIWNLIEPYIS"
     gene            919..2265
                     /locus_tag="P6L01_001918"
     CDS             919..2265
                     /locus_tag="P6L01_001918"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDP3030973.1"
                     /translation="MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILEN
                     VILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLR
                     VTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIAD
                     NAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIEN
                     AMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVL
                     IDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNE
                     WISMLGVVPVIRALREKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKD
                     PIKQAKELSSDKKSNEKLELFQNIFDIEAECPHEQAKQQKESKVKEISARRIFSFE"
     gene            2287..3102
                     /gene="ccsA"
                     /locus_tag="P6L01_001919"
     CDS             2287..3102
                     /gene="ccsA"
                     /locus_tag="P6L01_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HDP3030974.1"
                     /translation="MQENLFIRFNEIILLIYLISIICYFYDFVQKSHKIRSLGIYLLG
                     IVWVLQTISLSIFIIQTRHIPLGSISDVFYTLSWLIISISLILNLIKVLNFSVFFLNL
                     IGLTLLGMNTFQPTHYTNKVQKIAVVDELLLVHIGLAVLSYVFFALAFVNALLYIMQY
                     RNLKEKRFDQKYFRIGSVATLESIVFYSTLSGWIILIFSIILGTQWGVISVGERIFID
                     PKVILSSIITVLYGSYILLRINKWLNSRYLIYYNIILFCLNMINLFFATHFVN"
     gene            3144..4070
                     /gene="hemC"
                     /locus_tag="P6L01_001920"
     CDS             3144..4070
                     /gene="hemC"
                     /locus_tag="P6L01_001920"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDP3030975.1"
                     /translation="MRKLVVGSRRSKLALTQSQQFINKLKAVEPNLEIEIKEIVTKGD
                     RIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELP
                     FDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
                     DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKV
                     HNDEVAKCVTAERTFLAEMDGSCQVPIAGYATISDQKEIEFTGLIMTPDGKERFEYTM
                     NGTDPVELGKTVSNKLKEQGAYEIIKRLNEQH"
     gene            4092..4760
                     /locus_tag="P6L01_001921"
     CDS             4092..4760
                     /locus_tag="P6L01_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDP3030976.1"
                     /translation="MKPVVVMTQTNDMQSDLVSIIHKPFIDIKPLNFDIHLLNQRYDW
                     LIFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLKSF
                     NQTNQKILLPSSELARPLLLAALSKDNEVVKIDLYTSVPNKQNIQDVKEMIEHQQIDA
                     LTFSSSSAVRYYFNEGFVPKFKSYFAIGEQTARTIKTYQQPVTIAEIQTLESLIEKIL
                     ESRG"
     gene            4763..5737
                     /gene="hemB"
                     /locus_tag="P6L01_001922"
     CDS             4763..5737
                     /gene="hemB"
                     /locus_tag="P6L01_001922"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001633925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDP3030977.1"
                     /translation="MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKE
                     IKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATR
                     IAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
                     IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRK
                     TYQMDPANRLEALRELESDLKEGCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGE
                     YSMTKAAAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICRYLDK"
     gene            5785..7071
                     /gene="hemL"
                     /locus_tag="P6L01_001923"
     CDS             5785..7071
                     /gene="hemL"
                     /locus_tag="P6L01_001923"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDP3030978.1"
                     /translation="MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGS
                     KIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVI
                     DRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
                     TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGDDIAGVIVEPVAGNMGVVPPI
                     EGFLQGLRDITTEYGALLIFDEVMTGFRVGYHCAQGYFGVTPDLTCLGKVIGGGLPVG
                     AFGGKKEIMDHIAPLGNIYQAGTLSGNPLAMTSGYETLSQLTPETYEYFNMLGDILED
                     GLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVF
                     LPPSQFEGTFLSTAHTKEDIEKTIQAFDTALSRIVK"
BASE COUNT         2530 a          960 c         1357 g         2229 t
ORIGIN      
        1 acgctcaaat agtttaactt tgatcggagc ttttttataa tgtataccga aaggttcaaa
       61 tattatataa tggtagagtt gaaagagaat ataaaaaagt gaggatataa aatgaaagtt
      121 aatcctaata atattgaatt aatcattagt gcagtaaaag aagaacaata tccagaaaca
      181 gaattgtctg aagttgcact gagcggtcga tctaatgtag gtaagtctac atttatcaat
      241 agtatgattg gcagaaaaaa tatggcacgt acatcacagc aacccggcaa aacgcaaacg
      301 ttaaattttt ataatataga tgaacaactt atttttgtgg atgttccagg gtatggatat
      361 gctaaagtaa gtaaaacaca acgtgaaaaa tttgggaaaa tgattgagga atatataact
      421 aagagagaga atttgcaatt agttattcaa ttagttgatt taagacatga tccaacacaa
      481 gatgatatct taatgtacaa ttatttgaaa cattttgata ttcctacttt agttatatgc
      541 actaaagaag acaaaattcc aaaaggtaaa gttcaaaagc atattaaaaa tattaagaca
      601 caattagata tggacccaga cgatacaatt gtaagttatt catcaattca aaataataaa
      661 caacaacaaa tatggaattt aattgaaccg tatatttcat agtttttgta cgacaaaact
      721 tatacaaaaa ttttaaaaat aatgtaagca cgaaactttt aattagtaca caattgataa
      781 catttttcaa cgttcatcat tttgtcaaaa actcaaaagt aaattagaaa gattataatt
      841 tatttaagca tcgtacttaa ttggatttta aattatgtta taatatttgt attgttagta
      901 tatatggggg cgtttcaaat gcattttatt gcaattagta taaatcatcg cacagctgat
      961 gttgcactaa gagagcaagt tgcttttaga gatgatgcct tacgaattgc ccatgaagat
     1021 ttatatgaaa ctaaatctat tttagaaaat gtcatattat caacatgtaa tcgaactgaa
     1081 gtatatgctg ttgttgatca aattcacaca ggtcgttact atattcaacg atttctagct
     1141 cgtgcatttg gatttgaagt agatgatatt aaagcaatgt cagaagtaaa agtgggggac
     1201 gaagcagtag aacatttatt gcgtgtcact tctggtttag attcaatcgt acttggagaa
     1261 actcaaattt taggtcaaat aagagatgca tttttcttag cgcaaagcac aggtacgaca
     1321 ggaacaattt ttaatcatct atttaaacag gcaattactt ttgcaaaaag agcacataat
     1381 gaaacagata tagctgataa tgctgtaagt gtgtcttatg ctgcggtcga gttggcgaaa
     1441 aaagtatttg gcaaattgaa aagtaagcaa gctatcatta ttggtgcagg ggaaatgagt
     1501 gaattatcac tattaaatct tcttggttct ggaattactg atattacagt agtaaataga
     1561 acaattgaaa atgctatgaa attagcagca aagcatcaag tgaaatatga tgaactatca
     1621 tcattaccaa atttacttga aagtgcagat attgtgatta gttcaacgag tgcacaatct
     1681 tatatcatta caaatgaaat gatagaaaga attgcagaaa atagaaagca agattcacta
     1741 gtattgattg atattgcagt tcctcgagat attgaacctg gtattagtgc catcacaaac
     1801 atctttaatt atgatgttga tgacttaaaa ggtttagttg atgcaaactt acgtgagcga
     1861 caattagcgg ctgcaacaat ttcggaacaa attcctgcag aaatacatgc acacaatgag
     1921 tggattagta tgttgggtgt tgttccagtg attagagctt tacgtgaaaa agctatggca
     1981 attcaagcag aaacgatgga cagtattgat cgtaaattgc cagggttaag cgaaagagaa
     2041 cgtaaaatta tatctaaaca tacaaaaagt atcatcaatc aaatgttgaa agatcctatt
     2101 aaacaggcca aagaattaag tagtgataag aaaagtaatg aaaaattaga gctatttcaa
     2161 aatatatttg atatcgaagc tgaatgtcct catgagcaag cgaagcaaca aaaagaaagc
     2221 aaggtcaagg aaatttcagc gcgacgtatc tttagttttg aataagcata ttaatatggt
     2281 gataatatgc aagaaaacct gtttattcga ttcaatgaaa ttatattatt aatatactta
     2341 atcagtatca tttgctattt ttatgatttt gtccaaaaaa gtcataagat tagaagttta
     2401 ggcatatatt tattggggat tgtttgggtt ttacaaacaa tctctttatc tatttttatt
     2461 atacaaacta gacatattcc attagggtct atttcagatg tattctatac tttaagttgg
     2521 ctgattattt caatctcttt aattcttaac cttatcaaag tattgaattt ctctgtgttt
     2581 ttcttgaatt taatagggtt aacactttta gggatgaata catttcaacc gacacattat
     2641 actaataagg ttcagaaaat tgctgttgta gacgagttac tgttagtcca tatcggtcta
     2701 gctgtgttaa gttatgtctt ctttgcctta gcttttgtca atgcattact gtatatcatg
     2761 caataccgta atttgaagga aaaacgtttt gatcaaaaat actttagaat aggtagcgtt
     2821 gctacacttg aatcaattgt gttttactca acgttaagtg ggtggataat tctcatattt
     2881 agtattattt taggtacaca gtggggtgta atttcagttg gtgaacgtat attcattgac
     2941 ccgaaggtaa tactctcttc aattattaca gtattatatg gaagttatat attgcttaga
     3001 atcaacaaat ggttgaattc aaggtattta atatactata acattatttt attttgctta
     3061 aatatgatta atttattttt tgcaacacat tttgtgaatt gagaaaataa tctagagcaa
     3121 tccgttccta ggaggaattg aacatgcgta aattagtcgt tggctccaga agaagtaaat
     3181 tagctttaac acaaagccag caatttatta ataaattaaa agctgtcgag ccaaatctag
     3241 aaattgaaat taaagaaatt gtcacgaaag gcgatcgtat agtagataaa caattgtcta
     3301 aagtcggagg caaaggctta tttgttaaag aaatacaaca tgaacttttt gaaaaaaata
     3361 tcgatatggc aatacactcg cttaaagacg taccaagtgt aattccggaa ggtttaacat
     3421 taggttgtat ccctgataga gaattacctt ttgatgcgta tatttctaaa acacatacac
     3481 cactatccca attgccagaa ggcagtatta ttggtactag ttcattacgt cgtggtgcac
     3541 aaatattatc taagtatcct aatttagaga ttaaatggat tagaggtaat atagatacac
     3601 gattagaaaa gttacaaact gaagattatg atgcgattat tttagctgca gctggtttaa
     3661 gaagaatggg ctggtcagat gatattgtaa catcttatct tgatagagat acattgttac
     3721 ctgcaatcgg acaaggtgct ttagggatag aatgtcgtag tgacgatgaa gaactattaa
     3781 cattattaag caaagtacat aatgatgagg ttgcaaaatg tgtgactgct gaacgaacgt
     3841 ttttagcaga aatggatggt agttgtcagg tgccaatcgc aggatatgct acaatctcag
     3901 atcaaaaaga aatcgaattt acaggtttaa ttatgacccc agatggtaaa gaacgatttg
     3961 aatatacaat gaacggaaca gatccggttg agttaggcaa aacagtgagt aacaaattaa
     4021 aagagcaagg tgcttatgaa attataaaac gcttaaatga acaacattaa ataatggagt
     4081 gacgtttaaa tatgaagcca gttgtagtta tgacacaaac aaatgacatg caaagtgatt
     4141 tagtatcaat tattcacaaa ccctttattg atataaagcc actaaatttt gatattcatt
     4201 tgttaaatca acgctatgac tggcttattt tttcatctaa aaatgctgtg aaattctttt
     4261 ataaatatct aaaaggaatt aatgttgata acattgctgt gataggaagt aagacagcgc
     4321 aatattgtga atcacttggc attcgagttg attttatgcc aaacgacttt tctcaagaag
     4381 gatttttaaa atcatttaat caaactaacc aaaaaatact tttgccttcg agtgaattgg
     4441 cgagaccatt gttattagca gcgttatcta aagataatga agttgttaaa atagatttat
     4501 atacttcagt gcctaacaaa caaaatatac aagatgttaa agaaatgata gaacatcaac
     4561 aaatcgatgc attaacattt tcaagttcgt cggcagtacg ttattatttt aatgaaggat
     4621 ttgtaccaaa attcaagtcg tattttgcta ttggagaaca aacagcacgg accattaaaa
     4681 catatcaaca accagtaaca attgcagaaa ttcaaacact cgaatcacta attgaaaaga
     4741 ttttagaaag taggggctaa aaatgaaatt tgatagacat agaagattga gatcatcagc
     4801 gacaatgaga gatatggtta gagagaatca tgtaagaaaa gaagatttaa tatatccaat
     4861 ttttgtagtt gaaaaagacg atgtgaaaaa agaaattaag tcattgccag gtgtatacca
     4921 aatcagtttg aatttacttg aaagtgaatt aaaagaagct tatgacttag gcatacgtgc
     4981 cattatgttt ttcggtgttc caaactcaaa agatgatata ggtactggtg catacattca
     5041 cgatggtgtt attcaacagg caacacgtat tgctaaaaaa atgtatgatg acttattaat
     5101 tgttgcagac acttgtttat gtgaatatac tgatcatggt cattgtggcg tgattgatga
     5161 ccatacacat gacgttgaca atgataaatc attgccacta cttgttaaaa cagcaatttc
     5221 tcaagtggaa gctggtgctg atattattgc gccaagtaat atgatggatg gttttgttgc
     5281 tgaaattcgt cgtggattag atgaagccgg ctattacaat attcctataa tgagttatgg
     5341 tgtcaagtat gcatcaagtt tctttggacc ttttagagat gcagcagatt cagcgccatc
     5401 atttggggat agaaaaacgt atcagatgga ccctgctaac cgtttggaag cacttcgtga
     5461 attagaaagt gatcttaaag aagggtgcga catgatgatt gttaaacctg ctctaagtta
     5521 tttagacata gttcgagatg ttaaaaatca tacgaatgtt ccagttgttg catataatgt
     5581 gagtggagaa tatagtatga ctaaagcagc ggcacaaaat ggttggatag atgaagaacg
     5641 tgtcgttatg gaacaaatgg tttcaatgaa acgtgcaggt gctgatatga ttattacgta
     5701 ttttgcaaag gacatttgtc gctatttaga taaataaggt tttatatgta tgattttcca
     5761 taaactatag gaggaattta ctttatgaga tatacgaaat cagaagaagc aatgaaggtt
     5821 gctgaaactt taatgcctgg tggtgtaaat agtccagtac gcgcatttaa atcagtagat
     5881 acaccagcaa tttttatgga tcacggtaaa ggctcaaaaa tttatgatat cgatggtaac
     5941 gagtatatcg actatgtact aagttggggg ccgcttattt taggacatag agaccctcaa
     6001 gttattagtc atttacatga agcaattgat aaaggtacaa gttttggtgc atcaacatta
     6061 cttgaaaata aattggcgca gctcgttatt gaccgagtac cttcaataga aaaagtgcgt
     6121 atggtgtcat ctggtacaga agctacattg gatactttaa gattagcacg tggttatact
     6181 ggcagaaata aaattgtgaa atttgaaggt tgctatcatg gtcatagtga ttcgttatta
     6241 atcaaagctg gttctggggt ggcaacatta ggattgccgg attctcctgg tgtgcctgaa
     6301 ggtattgcta aaaatacaat tacagttcca tacaatgatt tagatgcact taaaatcgct
     6361 ttcgaaaaat ttggagacga tattgctggt gtaatcgtag aacctgttgc tggtaatatg
     6421 ggtgtcgtac caccgattga aggtttttta cagggattaa gagatattac gactgaatac
     6481 ggcgcattgc taattttcga tgaagtaatg actggtttca gagtcggtta tcattgtgca
     6541 caaggttact ttggtgtgac accagattta acttgcttag gaaaagttat cggtggagga
     6601 ctacctgtag gtgcttttgg tggtaaaaaa gaaatcatgg atcatatagc accattagga
     6661 aatatttatc aagcgggtac gttatcagga aatcctcttg caatgacaag tggttatgaa
     6721 acgttaagcc aattaacgcc agagacatat gagtatttta atatgttagg cgatatactt
     6781 gaagacggtt taaagcgtgt atttgctaaa cacaatgtac caataactgt aaatagagca
     6841 ggttcaatga ttggttattt cttaaatgaa ggacctgtaa ctaattttga acaagcgaat
     6901 aaaagtgatt tgaaattatt tgcagaaatg tatcgagaaa tggcaaaaga aggtgtgttt
     6961 ttaccaccat ctcaatttga aggtacattc ttatctacgg cacacacgaa agaagatatt
     7021 gaaaaaacga ttcaagcatt tgatacggct ttaagtcgta ttgtaaaata aatata
//
LOCUS       DAOBBH010000128         6909 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.128, whole genome shotgun sequence.
ACCESSION   DAOBBH010000128 DAOBBH010000000
VERSION     DAOBBH010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6909
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.128"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            55..3621
                     /gene="smc"
                     /locus_tag="P6L01_001924"
     CDS             55..3621
                     /gene="smc"
                     /locus_tag="P6L01_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDP3030979.1"
                     /translation="MVYLKSIDAIGFKSFADQTNVQFDKGVTAIVGPNGSGKSNITDA
                     IKWVLGEQSAKSLRGSKMEDIIFSGAEHRKAQNYAEVQLRLDNHSKKLSVDENEVIVT
                     RRLYRSGESEYYINNDRARLKDIADLFLDSGLGKEAYSIISQGRVDEILNAKPIDRRQ
                     IIEESAGVLKYKKRKAESLNKLDQTEDNLTRVEDILYDLEGRVEPLKEEAAIAKEYKT
                     LSHQMKHSDIVVTVHDIDQYTNDNRQLDQRLNDLQGQQANKEADKQRLSQQIQQYKGK
                     RHQLDNDVESLNYQLVKATEAFEKYTGQLNVLEERKKNQSETNARYEEEQENLMELLE
                     NISNEISEAQDTYKSLKSKQKELNAVIRELEEQLYVSDEAHDEKLEEIKNEYYTLMSE
                     QSDVNNDIRFLKHTIEENEAKKSRLDSRLVEVFEQLKDIQGQIKTTKKEYQQTNKELS
                     AVDKEIKNIEKDLTDTKKAQNEYEEKLYQAYRYTEKMKTRIDSLATQEEEYTYFFNGV
                     KHILKAKNKELKGIHGAVAEIIDVPSKLTQAIETALGASLQHVIVDSEKDGRQAIQFL
                     KERNLGRATFLPLNVIQSRVVATDIKSIAKEANGFISIASEAVKVAPEYQNIIGNLLG
                     NTIIVDHLKHANELARAIKYRTRIVTLEGDIVNPGGSMTGGGARKSKSILSQKDELTT
                     MRHQLEDYLRQTESFEQQFKELKIKSDQLSELYFEKSQKHNTLKEQVHHFEMELDRLT
                     TQETQIKNDHEEFEFEKNDGYTSDKSRQTLSEKETHLESIKASLKRLEDEIERYTKLS
                     KEGKESVTKTQQTLHQKQSDLAVVKERIKTQQQTIDRLNNQSQQTKHQLKDVKEKIAF
                     FNSDEVMGEQAFQNIKDQINGQQETRTRLSDELDKLKQQRIELNEQIDAQEATLQVCH
                     QDILAIENHYQDIKAEQSKLDVLIHHAIDHLNDEYQLTVERAKSEYTSDESIDALRKK
                     VKLMKMSIDELGPVNLNAIEQFEELNERYTFLSEQRTDLRKAKETLEQIISEMDQEVT
                     ERFKETFHAIQGHFTAVFKQLFGGGDAELQLTEADYLTAGIDIVVQPPGKKLQHLSLL
                     SGGERALTAIALLFAILKVRSAPFVILDEVEAALDEANVIRYAKYLNELSDETQFIVI
                     THRKGTMEFADRLYGVTMQESGVTKLVSVNLNTIDDVLKEEQ"
     gene            3621..4871
                     /gene="ftsY"
                     /locus_tag="P6L01_001925"
     CDS             3621..4871
                     /gene="ftsY"
                     /locus_tag="P6L01_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDP3030980.1"
                     /translation="MSFFKRLKDKFATNKENEEVKSLREEQGQDKLEDTHSEGSTQDV
                     NDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQ
                     LNNLIARYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREV
                     IVEKIVEIYHQEDDNSEAMNIEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVM
                     LAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDT
                     AGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVT
                     GIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQ
                     NDEITTVENDQIVTEEKDDNHGAK"
     gene            4858..5190
                     /locus_tag="P6L01_001926"
     CDS             4858..5190
                     /locus_tag="P6L01_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDP3030981.1"
                     /translation="MGQNDLVKTLRMNYLFDFYQSLLTNKQRNYLELFYLEDYSLSEI
                     ADTFNVSRQAVYDNIRRTGDLVEDYEKKLELYQKFEQRREIYDEMKQHLSNPEQIQRY
                     IQQLEDLE"
     gene            5216..6583
                     /gene="ffh"
                     /locus_tag="P6L01_001927"
     CDS             5216..6583
                     /gene="ffh"
                     /locus_tag="P6L01_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002457378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDP3030982.1"
                     /translation="MAFEGLSERLQATMQKMRGKGKLTEADIKIMMREVRLALFEADV
                     NFKVVKEFIKTVSERALGSDVMQSLTPGQQVIKIVQGELTKLMGGENTSINMSNKPPT
                     VVMMVGLQGAGKTTTAGKLALLMRKKYNKKPMLVAADIYRPAAINQLQTVGKQIDIPV
                     YSEGDQVKPQQIVTNALKHAKEEHLDFVIIDTAGRLHIDEALMNELKEVKEIAKPNEI
                     MLVVDSMTGQDAVNVAESFDDQLDVTGVTLTKLDGDTRGGAALSIRSVTQKPIKFVGM
                     SEKLDGLELFHPERMASRILGMGDVLSLIEKAQQDVDQEKAKDLEKKMRESSFTLDDF
                     LEQLDQVKNLGPLDDIMKMIPGMNKMKGLDKLNMSEKQIDHIKAIIQSMTPAERNNPD
                     TLNVSRKKRIAKGSGRSLQEVNRLMKQFNDMKKMMKQFTGGGKGKKGKRNQMQNMLKG
                     MNLPF"
BASE COUNT         2704 a          904 c         1365 g         1936 t
ORIGIN      
        1 aatattaaaa tatcgtatac atgtgttctt taaaattgtg ataaggagtt taggatggtt
       61 tatttaaaat caatagatgc cattggattt aagtcttttg cagatcaaac caatgttcaa
      121 ttcgataaag gtgtaactgc aattgttggt ccaaatggaa gcggtaaaag taatattaca
      181 gatgctatta aatgggtgtt gggtgaacaa tcggctaaat cattacgtgg ctcaaaaatg
      241 gaagatatta tcttctcagg tgcagaacat cgcaaagctc aaaattatgc tgaagtacag
      301 ttaagattag ataatcattc taaaaagctc agtgttgatg aaaacgaagt tattgtaaca
      361 agaagattgt atcgaagtgg tgaaagtgag tactacataa ataatgaccg tgcaagatta
      421 aaagatattg ccgatttatt tttagattct ggattgggaa aagaagcgta tagcattatc
      481 tcgcaaggta gagttgatga aatactaaat gctaaaccaa ttgatagacg tcaaattatt
      541 gaagaatcgg ctggtgtact taaatataaa aaacgtaagg ctgaatcatt aaataaactt
      601 gaccaaacag aagataattt aacgagagta gaagacattt tatatgattt ggaaggtcgc
      661 gtagaacctc taaaagagga ggcagctata gctaaagaat ataagacact ttcacatcaa
      721 atgaaacata gtgacattgt agttacagtg cacgatattg atcaatatac aaatgacaat
      781 agacaattag atcaacgttt aaatgattta caaggtcaac aagcaaataa agaagctgac
      841 aagcaacgtt taagccaaca aattcaacaa tataaaggta aacgacatca acttgataac
      901 gatgttgaat cgcttaatta tcaattagta aaagctacgg aagcctttga aaaatatacg
      961 ggacaattaa atgttttaga agaacgtaag aaaaatcaat ctgaaacaaa tgcacgatat
     1021 gaagaagaac aagaaaattt aatggagctt ttagaaaata tatcaaatga gatttctgaa
     1081 gctcaagata cttataagtc tctgaaaagt aaacaaaaag aactcaatgc tgtcattcgt
     1141 gaacttgaag aacaactata tgtttcagac gaagcacatg atgaaaaatt ggaagaaatt
     1201 aaaaacgaat actatacatt aatgtcagag caatcagatg ttaacaatga tattcgtttt
     1261 ttaaagcata ctatagaaga gaatgaggct aaaaaatcaa gactagattc tcgattagtt
     1321 gaagtttttg agcaattgaa agatattcag ggtcaaataa aaacgacaaa aaaagaatat
     1381 caacagacca acaaagaact ttctgctgta gataaagaaa ttaaaaatat agaaaaagat
     1441 ctcactgata caaaaaaagc acaaaatgaa tacgaagaga aattgtatca agcatatcga
     1501 tataccgaaa aaatgaaaac acgtattgat agtttggcaa cgcaagagga agaatacact
     1561 tattttttca atggcgtcaa acatattttg aaagctaaaa ataaagaatt aaagggtatt
     1621 catggtgcag ttgcggaaat tattgatgtg ccatctaaat taactcaggc aattgaaaca
     1681 gcattaggtg cttcattaca acatgtcatt gtagattcag aaaaagatgg acgccaggct
     1741 attcaatttt taaaagaacg taatttaggt cgtgcgacgt ttttaccatt aaatgttata
     1801 cagagtagag tggtagcgac tgatattaaa tctattgcta aagaggcaaa cggatttatt
     1861 agtattgctt cggaagcagt gaaagtagca ccagaatatc aaaatattat cgggaattta
     1921 ttaggtaata cgattatcgt tgatcattta aagcatgcaa atgaattggc acgtgcgatt
     1981 aaatatcgaa ctcgtattgt tactttggaa ggtgatattg taaaccctgg tggttctatg
     2041 actggtggtg gcgctcgtaa gtccaaaagt attctgtctc aaaaagacga gttgacaaca
     2101 atgagacacc aattagaaga ttacttgcgt caaacagaat catttgaaca acaatttaaa
     2161 gagttgaaga taaaaagtga tcaattaagt gaactgtatt ttgaaaaaag tcaaaagcat
     2221 aatacactta aagagcaagt gcatcatttt gaaatggagc tcgatagatt aactacacaa
     2281 gaaacacaaa taaaaaatga tcatgaagaa ttcgaatttg aaaaaaatga tggttatacg
     2341 agtgacaaaa gtcgacaaac tttgagtgaa aaagaaactc atttagaaag tattaaagca
     2401 tctttaaaac gactagaaga tgaaattgaa cgctacacaa aactttctaa agaaggtaag
     2461 gaaagcgtaa ctaaaacaca acaaacgtta catcagaaac aatctgatct tgctgtggtt
     2521 aaagagcgta ttaaaacaca acaacagaca atagatcgat taaataatca aagtcaacaa
     2581 actaaacatc aattaaaaga tgttaaagaa aaaattgcat tctttaattc ggatgaagtg
     2641 atgggcgaac aagcttttca aaatattaaa gatcaaatta atggtcaaca agaaacgaga
     2701 acacgcttat ctgatgaatt agataaattg aaacaacaac gtattgagtt gaatgaacaa
     2761 atcgatgcgc aggaagctac actacaagtt tgtcaccaag atattttagc tatcgaaaat
     2821 cactaccaag atattaaagc tgaacaatca aagttagatg tattaattca tcatgcgata
     2881 gatcatttaa atgatgaata tcaattgact gttgaacgtg cgaaatctga atatacgagt
     2941 gatgaatcga ttgacgcatt acgtaaaaaa gttaagttaa tgaagatgtc gattgatgaa
     3001 ctaggtcctg taaacttaaa tgcaattgaa caatttgaag agttaaatga acgttataca
     3061 tttttaagtg aacaacgtac agatcttcgt aaagctaaag aaacattaga gcaaattata
     3121 agtgaaatgg atcaagaggt tactgaaaga tttaaagaaa ctttccatgc tattcaagga
     3181 cattttacag ctgtgttcaa acaattgttt ggtggaggcg atgcagaatt gcaattaact
     3241 gaagccgatt atttaacagc tggtattgat attgtggtac aaccaccggg taaaaagttg
     3301 caacatttat cgttactgag tggtggtgag cgtgcattaa ctgctattgc tttactattt
     3361 gcaattttaa aagtaagatc tgcacctttt gttatattag atgaggttga agctgcacta
     3421 gatgaagcaa atgttattag atacgcaaaa tatttaaatg agttatcaga cgaaacacaa
     3481 ttcattgtta ttacacaccg taaaggaaca atggaatttg cagataggtt atacggtgta
     3541 acaatgcaag aatcaggtgt tactaaactt gtgagtgtga atttaaatac aatagatgat
     3601 gtgttgaagg aggagcaata atgagctttt ttaaacgctt aaaagataag tttgcaacaa
     3661 ataaagaaaa tgaagaagtt aaatccttaa gagaagaaca aggtcaagac aaattagaag
     3721 atacacattc tgaaggttca acgcaggacg taaatgattt agcagaaaat gctgaagtga
     3781 aaaagaagcc acgcaagttg agtgaagcgg attttgatga cgatggctta atatcaattg
     3841 aagattttga agaaattgaa gctcaaaaaa tgggtgctaa atttaaagca ggactcgaaa
     3901 aatctcgtca aaatttccaa gaacaattaa ataatttgat agcgagatat cgtaaagtag
     3961 atgaagactt ttttgaagct ttagaagaaa tgttaatcac tgcagacgtc ggttttaata
     4021 cagtgatgac gttaactgaa gaattacgta tggaagcaca acgacgtaat attcaagata
     4081 ctgaagattt gcgtgaagtc attgttgaaa aaatcgtaga gatttaccat caagaagatg
     4141 ataattcaga agctatgaac atagaagatg gtcgtttaaa tgtcatttta atggtcggtg
     4201 tgaatggtgt tggtaaaaca acaacaattg gaaaattagc ttaccgatat aaaatggaag
     4261 gtaaaaaagt aatgttagct gcgggcgata cttttagagc gggtgctatt gatcaattga
     4321 aagtttgggg cgaacgtgtt ggtgtagatg taattagcca aagtgaaggt tctgatccag
     4381 ctgctgttat gtatgatgcg attaatgccg ctaaaaacaa aggtgttgat attttaatct
     4441 gtgataccgc tggacgttta caaaataaaa caaatctaat gcaagaatta gaaaaagtta
     4501 agcgtgtaat taatcgagca gtgccagatg cgcctcatga agcattacta tgtttagatg
     4561 ctacaactgg tcagaatgcg ttgtcacaag ctagaaactt taaagaagta acaaatgtta
     4621 caggtattgt attaacgaaa ttagatggta cagccaaagg tggtatcgta ttagccattc
     4681 gtaatgaatt gcacattcca gttaaatatg taggtttagg tgagcaatta gatgacttac
     4741 aaccatttaa ccctgaaagt tatgtctacg gcttattcgc tgatatgatt gaacaaaatg
     4801 acgaaataac aacagttgaa aatgatcaaa ttgtaacaga agaaaaggac gataatcatg
     4861 gggcaaaatg atttagttaa aacgttacga atgaattatt tgtttgattt ttatcaatcc
     4921 ttattgacga ataaacaacg taattatttg gaattatttt atctcgaaga ttattcttta
     4981 agtgaaatcg cagatacttt taatgtgagt agacaagcag tttatgataa tataagaaga
     5041 actggcgatt tagttgaaga ttatgaaaag aaattggaat tataccagaa atttgagcaa
     5101 cgccgagaaa tatatgatga aatgaaacaa catttaagta atccagaaca aatacaacgt
     5161 tatattcaac aattagaaga cttagaatag atataaattg agggaggaca tcgatatggc
     5221 atttgaaggc ttatcagaac gcctgcaagc gacgatgcaa aaaatgcgtg gtaagggtaa
     5281 acttactgaa gctgatataa agataatgat gcgtgaagta agattagcgt tatttgaggc
     5341 tgacgtaaac tttaaagtgg taaaagaatt tattaaaaca gtatcagaac gcgcattagg
     5401 ttccgatgta atgcaatcat taacaccagg gcaacaagtt attaaaatag ttcaaggtga
     5461 attaacgaag ttgatgggtg gagaaaatac atcgattaat atgtcaaata aaccacctac
     5521 tgttgttatg atggttggtt tacaaggtgc tggtaaaaca acaactgcag gtaaattagc
     5581 attattgatg cgtaaaaaat acaacaaaaa acctatgtta gttgcagcag atatttatcg
     5641 tccagcagcg ataaatcaat tacaaacagt agggaaacaa attgatattc ctgtatacag
     5701 tgaaggagat caagtaaagc cacaacaaat tgtaactaat gcattaaaac atgctaaaga
     5761 agaacattta gactttgtaa tcattgatac agcaggtcga ttacacatcg atgaagcatt
     5821 gatgaacgaa ttaaaagaag taaaagaaat tgctaaacca aacgaaatta tgttagttgt
     5881 cgattcaatg acgggtcaag atgctgtcaa tgttgcagaa tcttttgacg atcaacttga
     5941 tgtcacaggt gttaccttaa ctaaattaga tggtgataca cgtggtggtg cagctttatc
     6001 tattcgttcg gtgacacaaa aaccaattaa atttgttggt atgagtgaaa agttagatgg
     6061 tttagagcta ttccatcctg aacgtatggc atcacgtatt ttaggtatgg gtgatgtgtt
     6121 aagtttaatt gaaaaagcgc aacaagatgt ggatcaagaa aaagcaaaag atttagagaa
     6181 aaagatgcgt gagtcatcgt ttactttaga tgatttttta gaacaacttg atcaggtgaa
     6241 aaatctagga ccactggatg atattatgaa aatgattcca ggtatgaata aaatgaaagg
     6301 gctagataag cttaatatga gtgaaaagca aattgatcat attaaagcga ttatccagtc
     6361 aatgacgccg gctgaaagaa acaatccaga cacattgaat gtatcacgta aaaagcgtat
     6421 tgctaaaggg tctggtcgtt cattacaaga agtcaatcgt ttgatgaaac aatttaacga
     6481 tatgaagaaa atgatgaaac aattcactgg tggcggtaaa ggtaaaaaag gtaaacgcaa
     6541 tcaaatgcaa aatatgttaa aaggtatgaa tttaccgttt taatgtttaa tataacacgc
     6601 tgatttttgt tgcaatgaga tgtgcatccc gaaagttata ctaaagaata taactttatg
     6661 gggtgcatat ttttagatac acaataataa ttaaaaactg aaattgcgga tttgctagaa
     6721 catgaggtta ttgttgctaa tattaataag ttatattggt ggaacatgtg cgttagaagt
     6781 ggtaccattc atgtccttac gagataaatt aacttaacac gtaggtggtg aaggctacta
     6841 tgaattttta taacctatta acagtctaat ttagtaatcg catagttatt tttgtaagaa
     6901 aaaggttca
//
LOCUS       DAOBBH010000129         6897 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.129, whole genome shotgun sequence.
ACCESSION   DAOBBH010000129 DAOBBH010000000
VERSION     DAOBBH010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6897
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.129"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..525
                     /locus_tag="P6L01_001928"
     CDS             <1..525
                     /locus_tag="P6L01_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDP3030983.1"
                     /translation="FWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKVV
                     ATDEVMTSPSFIKQKWFGEQRTPVYVYKWEDVQIQHGIGDLHIDLTKAANIKENNTIV
                     VRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHIEEMMKPDNYTVNI
                     YVSTFIGDVEVIYR"
     gene            522..1565
                     /locus_tag="P6L01_001929"
     CDS             522..1565
                     /locus_tag="P6L01_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDP3030984.1"
                     /translation="MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIF
                     GIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYS
                     ETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVS
                     QQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLG
                     EGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVE
                     LFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIE
                     VKAPLNKEDSYDD"
     gene            1555..2184
                     /gene="vraR"
                     /locus_tag="P6L01_001930"
     CDS             1555..2184
                     /gene="vraR"
                     /locus_tag="P6L01_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator VraR"
                     /protein_id="HDP3030985.1"
                     /translation="MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKA
                     HELKPDLILMDLLMEDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSY
                     ILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
                     KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ"
     gene            complement(2297..3514)
                     /locus_tag="P6L01_001931"
     CDS             complement(2297..3514)
                     /locus_tag="P6L01_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDP3030986.1"
                     /translation="MSKKDHSSSKYLNSVKEAQEESKKKNKSNPKIDVDRTYIEPQQF
                     QSKKPKKDDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMF
                     PMLLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILA
                     IWSASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTLGSVI
                     SHFLFGPLGFDEQVKWIFNLIRIVLPIIIIFIIFIVLYSVAPNVKTKLKSVLPGAVFT
                     SIIWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQR
                     SVIKGKTPEEAALEHDDNNKNHYNENTRYEYDEDSNATHQRTYHVDEHPSDTYPEDDK
                     NIKDKIVDKLKKD"
     gene            complement(3792..4067)
                     /locus_tag="P6L01_001932"
     CDS             complement(3792..4067)
                     /locus_tag="P6L01_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDP3030987.1"
                     /translation="MENKFVPGILIGAVIGGAISLADKSTRQALVQSVKDAKNGNRTR
                     KPSKVSKIKDEVLYWKDVVEEIRRNNPELERSLKDAKETFVNRKNQR"
     gene            complement(4074..4538)
                     /locus_tag="P6L01_001933"
     CDS             complement(4074..4538)
                     /locus_tag="P6L01_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDP3030988.1"
                     /translation="MVDVAFVCLGNICRSPMAEAIMRQRLKDRNIHDIKVHSRGTGSW
                     NLGEPPHEGTQKILNKHNIPFDGMISELFEATDDFDYIVAMDQSNVDNIKSINPNLKG
                     QLFKLLEFSNMEESDVPDPYYTNNFEGVYDMVLSSCDNLIDYIVKDANLKEG"
     gene            4685..4885
                     /locus_tag="P6L01_001934"
     CDS             4685..4885
                     /locus_tag="P6L01_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1128 family protein"
                     /protein_id="HDP3030989.1"
                     /translation="MTNEEKVLAIREKLNIVNQGLLDPEKYKNANEEELTDIYDFVQS
                     RERLSPSEVTAIADALGQLRHD"
     gene            4888..6144
                     /locus_tag="P6L01_001935"
     CDS             4888..6144
                     /locus_tag="P6L01_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDP3030990.1"
                     /translation="MGILTNYKEKLQQYAELLVKVGMNVQPKQPVFIRSSVETLELTH
                     LIVEEAYHCGASDVRVVYSDPTLKRLKFENESVEHFANHEIKSYDVEARMDYVKRGAA
                     NLALISEDPDLMDGIDSQKLQAFQQQNARAFKGYMESVQKNQFPWVVAAFPSKAWAKR
                     VYPELSVEEAYIKFIDEVFDIVRIDGNDPVENWRQHIANLSVYAQKLQQKNYHALHYV
                     SEGTDLTVGLAKNHIWEDATSYVNGEEQAFIANIPTEEVFTAPDRNRVDGYVTNKLPL
                     SYNGTIIDQFKLMFKDGEIIDFSAEKGEAVLKDLINTDEGSRRLGEVALVPDDSPISN
                     RNTIFYNTLFDENAACHLAIGSAYAFNIQGGTEMTVEEKIASGLNDSNVHVDFMIGSS
                     DLTIYGIFEDGSKELVFENGNWASTF"
     gene            6234..6764
                     /locus_tag="P6L01_001936"
     CDS             6234..6764
                     /locus_tag="P6L01_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDP3030991.1"
                     /translation="MTNQDRPMKSMSESKCYKNRQVFPQDTNHHHTMFGGTLMANIDE
                     IAAITAMKHAGAQVVTASTDSVDFLKPIKTGDILQYVAMVSYAGTSSMEVVVQIRIDD
                     VFNNKHDLAALSYLTFVALDDEGKPKHVPGVYPEDDVEKWFYDTAPQRVERRKARRIE
                     SKQTIEYLAQVQHIRD"
BASE COUNT         2439 a         1018 c         1214 g         2226 t
ORIGIN      
        1 ttttggatag gcgcgctatt aattgcattc acattattgt ctaataagta tacaatcatc
       61 atcttgttcg tctttttatt actacttatt gtgcgttatt taatacataa atttaaacca
      121 aaaaaagtag ttgcgacgga tgaggttatg acttcaccat cttttattaa acaaaagtgg
      181 tttggtgagc aacgtacacc agtttatgta tataagtggg aagatgtcca aattcaacat
      241 ggaattggcg acctacatat tgacttaaca aaagctgcaa atattaagga aaataatacc
      301 attgttgtta gacacatttt aggtaaagtg caggttatat tgccggttaa ttacaatatt
      361 aatttacatg tagctgcttt ttatggaagt acttacgtga atgaaaaatc atataaagtt
      421 gaaaataaca atattcatat tgaagaaatg atgaaaccgg ataactatac agttaatatc
      481 tacgtatcaa cgtttatcgg agacgtagag gtgatttatc gatgaaccac tacattagaa
      541 caattggttc aatgctcatc ttagtatata gcatgctagc tgcatttcta ttcatcgata
      601 aagtttttgt aaatatcatc tattttcaag gtatgtttta tacacaaata ttcggaatac
      661 cagtcttttt atttttaaat ctcatcatca tattattgtg tattattgtt ggttcggtac
      721 tcgcatacaa aatcaatcag caaaatgatt ggattaagac gcaaattgag cgttcaatgg
      781 aaggcgaaac agttggcatt aatgatcaaa atatagaaat atatagtgaa acgttagatt
      841 tataccatac actcgtacct ttaaatcaag aattgcataa gttgcgactt aaaactcaaa
      901 acttaaccaa tgaaaattat aatattaatg atgtgaaagt taaaaagatt attgaagatg
      961 aacgtcaaag actagctcga gaacttcacg attctgttag tcagcaactt tttgcggcaa
     1021 gtatgatgct ttctgctatc aaagaaacga agttagaacc accattagac caacaaattc
     1081 cgattttaga gaaaatggtt caagattcgc agttagaaat gcgtgctttg ctgttacatt
     1141 taagaccgct tggtttaaaa gacaaatctt taggtgaggg tattaaagat ttagttattg
     1201 atttacaaaa aaaagtgcca atgaaagttg tgcatgaaat acaagatttt aaagtgccta
     1261 aaggtattga agatcatttg ttcagaatta cacaggaagc aatttcgaat acattgcgtc
     1321 attcaaacgg tacaaaagtg acagtagaat tgtttaataa agacgattat ttattgttga
     1381 gaattcaaga taatggtaaa ggttttaatg ttgatgaaaa attagaacaa agttatggac
     1441 ttaaaaatat gcgtgaaaga gctttggaaa ttggtgcaac gttccatatt gtatcattgc
     1501 cagattcagg tacacgtatc gaggtgaaag cacctttaaa taaggaggat tcgtatgacg
     1561 attaaagtat tgtttgtgga tgatcatgaa atggtacgta taggaatttc aagttatcta
     1621 tcaacgcaaa gtgatattga agtagttggt gaaggcgctt ctggtaaaga agcaattgcc
     1681 aaagcccatg agttgaagcc agatttaatt ttaatggatt tacttatgga agacatggat
     1741 ggtgtagaag cgacgactca gattaaaaaa gatttaccgc aaattaaagt attaatgtta
     1801 actagtttta ttgaagataa agaggtatat cgtgcattag atgcaggtgt cgatagttac
     1861 attttaaaaa caacaagtgc aaaagatatc gccgatgcag ttcgtaaaac ttctagagga
     1921 gaatctgttt ttgaaccgga agttttagtg aaaatgcgta accgtatgaa aaagcgcgca
     1981 gagttatatg aaatgcttac agaacgagaa atggaaatat tattattgat tgcgaaaggt
     2041 tactcaaatc aagaaattgc tagtgcatcg catattacta ttaaaacggt taagacacat
     2101 gtgagtaaca ttttaagtaa gttagaagtg caagatagaa cacaagctgt catctatgca
     2161 ttccaacata atttaattca atagttcgta tcgaattaag aaaagttact tacgccaatc
     2221 acaatataac atcaaataga caccaaaaaa cagaggtctt cgcaatcatt agcgaaaacc
     2281 tctgttttgt tggtgtttag tcttttttaa gcttatcaac gattttgtct tttatatttt
     2341 tatcatcttc tggataggta tcactaggat gttcatcaac atgatacgta cgttgatgtg
     2401 ttgcattact atcttcatca tattcgtatc tagtgttctc attataatga tttttattat
     2461 tgtcatcatg ttctaatgct gcttcttcag gtgttttacc tttaattaca ctacgctgat
     2521 gaatgattgc attgatttca gcaccgacaa ttataataaa acttgtgata tataaccata
     2581 gtaataaaat gatgataccc gcgatactgc catatgtttt agaatagtta ccaaaatttg
     2641 aaatatacca accaaaacca aatgaaccag ctaaccaaat aattgaagta aatactgcac
     2701 ctggtaatac tgacttaagc ttcgttttaa cgttaggtgc aaccgaatat aacacgataa
     2761 atatgataaa tataataatg attggtaaca caattctaat aaggttaaaa atccatttca
     2821 cttgttcgtc aaatccaagt ggaccgaata ggaaatgact aattacagaa ccaagcgttg
     2881 gtaatgctag agcaactaca aacacaacgc ccataactac agtgaagaca acacttagta
     2941 gttttaaaac gattccatta cggctatctt ctacatcgta agcaacattg aaagaattca
     3001 taattgcagt cattccattt gaagctgacc aaattgctaa aatcaaaccg atagataata
     3061 agccaccact ggagttttga gttatatcac caattacact cttaattaga gtagatgttt
     3121 cagcgggtgc attgcttaac atattagtaa tttgactctg cttaatattg aaaaatggta
     3181 ataatgttaa taggaaaagc aacataggga acatagcaag tacgaaatgg taagtcattt
     3241 gcgctgctag tcctgaggca tcatcttttc ctatgcgata gatgagatat gataagaaat
     3301 tagagtcttt cttatatttt gcaggtttat ttaatcttga taagaagaaa acctgatcat
     3361 cttttttagg tttcttagat tggaattgtt gaggttcaat atatgtacga tcaacatcaa
     3421 ttttgggatt acttttattt ttcttttttg actcctcttg cgcttcctta acagaattaa
     3481 ggtattttga agaagagtga tcctttttcg acatgtgcac tccttaacct ttctctatgt
     3541 aatgttttat aaagtaacta ttagtattta tccaattata aaaaaactaa acaatgatat
     3601 gctttaaaaa tgataatatt atagaaatta attgatttaa atctttcata ggctattttg
     3661 atttaaaatt aaaacaaagg caatactgaa acaatgtagt ttggccaatc attgtattat
     3721 catttaataa tgataactat gaaaggcgta caccaaattg ttaagtattg ccttataaaa
     3781 acaaaatcat cttagcgttg attttttcta ttaacaaatg tttctttcgc atcctttaat
     3841 gaacgttcta attcaggatt attacgacga atttcttcaa caacatcttt ccagtataaa
     3901 acttcgtctt taatcttgct gactttagaa ggcttacgag tgcggttacc attttttgca
     3961 tctttaactg attgaactaa agcttgacgt gtagatttat cagctaaact aattgcacca
     4021 ccaattacgg caccaattaa aataccagga acaaatttat tttccataaa aaactacccc
     4081 tctttcaaat ttgcatcttt tacgatgtag tctattaaat tatcacaaga tgataatacc
     4141 atgtcgtata caccttcaaa attattcgtg tagtatggat ctggtacatc actctcttcc
     4201 atattactaa attctaacag tttgaacaat tgtcccttaa gattaggatt gatagattta
     4261 atattatcaa cgttactttg atccatagcc acaatgtaat caaaatcatc tgtcgcttcg
     4321 aataattcac taatcatgcc atcaaatgga atattgtgtt tgttgagaat tttttgtgta
     4381 ccttcatgag gcggctctcc taaattccag ctaccagtac ctcttgaatg tactttaata
     4441 tcatgaatat ttctgtcttt aagtctttgt cgcatgattg cttctgccat tggagaacga
     4501 catatattgc caagacagac aaatgctaca tctaccattt tgattcctcc aaactatgta
     4561 gttatatccc cattttatag cgactttaaa caataagaaa gcagattata taaaattcta
     4621 ttaaagttta ttaaattgtg atactttgat aacataacta ttattagagg tgaacattgt
     4681 ggctatgaca aatgaagaga aagtattagc tattagagag aagttaaata ttgttaatca
     4741 aggattatta gatcctgaaa aatataaaaa tgcaaatgaa gaagaattaa cagatatata
     4801 tgattttgtt caatcaagag aaagattgtc gccaagtgaa gtgacagcta ttgctgacgc
     4861 tttaggacaa ttgcgacacg actaggagtg ggaattttga ctaattacaa agaaaagtta
     4921 caacaatacg ctgaactatt agttaaggtt ggtatgaatg ttcaaccaaa acagccagta
     4981 tttattagat catcagtaga aacattagaa ttaacgcatt taattgtaga agaagcatat
     5041 cattgtggcg cttcagatgt acgtgttgta tatagcgatc caaccttaaa acgcttaaaa
     5101 tttgaaaatg aatctgtgga acattttgct aatcatgaga taaaatcata tgatgttgaa
     5161 gcgagaatgg attacgttaa acgtggcgct gctaatttag cacttattag tgaagatcct
     5221 gatttaatgg atggtatcga tagccaaaaa ttacaagcat ttcaacaaca aaacgcacgc
     5281 gcatttaaag gatatatgga aagtgtacaa aaaaatcaat ttccatgggt cgtagctgct
     5341 tttccatcta aagcttgggc gaagagagta tatcctgaac tatctgtaga agaagcatat
     5401 attaagttta ttgacgaggt atttgatatt gtgcgaattg atggtaacga tccagttgaa
     5461 aattggcgtc aacacatcgc taatttaagt gtttacgctc aaaagttaca acaaaaaaat
     5521 taccatgcgt tgcattatgt atctgaaggt actgatttaa cagtaggttt agcgaaaaat
     5581 catatctggg aagatgctac aagttatgtt aacggcgagg aacaagcttt tattgctaat
     5641 ataccaactg aagaagtatt tacagcacca gatcgaaata gagtagatgg atatgttact
     5701 aataaattgc ctttaagtta taatggaacg attatcgatc aatttaaatt gatgtttaaa
     5761 gatggagaaa ttattgattt ttcagctgaa aaaggtgaag cggtattgaa agatttaatc
     5821 aataccgatg aaggttcaag aagattaggt gaagtagcgt tagtacctga tgattcaccg
     5881 atatcaaatc gtaataccat tttttacaat actttatttg atgaaaatgc agcatgtcat
     5941 ttagcgattg gatctgctta cgcctttaat attcaaggtg gaacggaaat gactgttgag
     6001 gaaaagattg caagcggatt aaatgattca aacgtacatg tcgatttcat gattggtagt
     6061 agtgatttga ctatttatgg catattcgaa gatggttcaa aagaactagt atttgaaaat
     6121 ggaaattggg catcaacatt ttaataaatg ttattttgag gtgctgagta ggaaatgaaa
     6181 catgtattta aaggtcaaat acgtgattaa agtatagatt gggagataaa ataatgacaa
     6241 atcaggacag accaatgaaa tctatgtcag aatcaaaatg ttataaaaat agacaagttt
     6301 tccctcaaga tacgaatcac catcatacaa tgtttggtgg tacattgatg gctaatattg
     6361 atgaaattgc agcaatcaca gctatgaaac atgctggtgc gcaagtagtt accgcatcta
     6421 cagactcagt agatttctta aagccgatta aaacagggga catattacaa tacgtagcga
     6481 tggtttcata cgctgggact agttcaatgg aagtggtcgt tcaaattaga attgacgatg
     6541 tatttaataa caagcatgat ttagctgcat taagttactt aacatttgtt gcgttagatg
     6601 atgaaggcaa acctaaacat gtacctggtg tatatccaga agatgatgtt gaaaaatggt
     6661 tttatgatac agcaccacaa cgtgttgaac gaagaaaagc acgtcgcatt gaaagtaaac
     6721 aaacaattga atatttagct caagtgcaac atattagaga ctaaaaatat aaagacagat
     6781 tgttaaggat gaattgaact gttatgttcg aatattccac ctacaatctg tctttttgat
     6841 tatgcacgtt tatgctttta gttaaaattt aaccttttta ttatagacta ttagtcc
//
LOCUS       DAOBBH010000130         6871 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.130, whole genome shotgun sequence.
ACCESSION   DAOBBH010000130 DAOBBH010000000
VERSION     DAOBBH010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6871
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.130"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            92..394
                     /locus_tag="P6L01_001937"
     CDS             92..394
                     /locus_tag="P6L01_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HDP3030992.1"
                     /translation="MTKNYKDMTQEEIKDLLSEKTAELYELAKEIKGESKFDILLFSS
                     IGVIDGDYLAGSSSVIGHTFDLAYLLDSTKSYKDIVNVLQMCKSQKILGIDDDKED"
     gene            399..659
                     /locus_tag="P6L01_001938"
     CDS             399..659
                     /locus_tag="P6L01_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HDP3030993.1"
                     /translation="MYYKTGDVCRKIFNVDGFDFQLRVKKRAYSVEIVVLDHEGNSID
                     GLLVSDENDLYTALDILKQSIYEWIENNTDEQDRLINLVMKW"
     gene            668..931
                     /locus_tag="P6L01_001939"
     CDS             668..931
                     /locus_tag="P6L01_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001638663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3030994.1"
                     /translation="MRDTERNILNIFKTLFDEYTLSNQRALLEIERNHHGYLSINFLH
                     YHDSYKTNNKLVQIHEINPDSHERIKNLIIEVLRGHRKIKKGA"
     gene            940..2883
                     /locus_tag="P6L01_001940"
     CDS             940..2883
                     /locus_tag="P6L01_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDP3030995.1"
                     /translation="MKINKLTISNFAGIKEEKFNFDGKDAKIYGNNATGKTTTATALQ
                     WLLFDKGLDGSTKSFNPVPLNEKNEENYELIPTVFAEFEIDGKITTFKKESHPKYTIN
                     QKTNRKEYSRSRTKKQYINDESIKVKDYKARIDELIDEDVFKLITNPQAFNLLDWKKR
                     RSLLFEIAKPINDEDVIKTNDDFKELNNILGDHEIETKKKILTDKIKQINKDIKDIPI
                     RINQTQQNKQDVPEFDNDRHTIIKQEIEQLENERIDIQNGAEEINLRNQLADKQSELK
                     RIEANNSASNENKIHALTNELHVENGTVANLKTRLKQNKQQITHEENRRNQLLENHKG
                     LKSDLEKAKNQKFEYLDDNVCSCCGQQLPAEQVSEVREKALQKFNANKSKELETIQTS
                     INHIISEGKKIKPIIEKLEDDNNNLQIKINEAEERSARIQNKINKLKTTHVDVTQTDE
                     YKAVMLEINEINQKRSNIRKTIQDKVSGIDDKISELTQEKSEIEVSISIEKSNKHLDD
                     VISELRNEEDRLLDEKEKYSHDLYILKEFTTTKVKMLTENINNEFDIAEFKLFNTLVN
                     GELEETCSTTVNGVEYDSGLNNASRINVGLDIINTLSKHFKVTAPIFIDNAESVTELI
                     KTESQQIQLIVNEQDKKLRMETI"
     gene            2885..3805
                     /locus_tag="P6L01_001941"
     CDS             2885..3805
                     /locus_tag="P6L01_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDP3030996.1"
                     /translation="MTENNKLQTIEQQLVQEKNVSDNVLNKVRVLESQGNLELPNDYS
                     PSNAMKQAWLQISQDNKLMSCNDTSKANALLDMVTQGLNPAKNQCYFIPYGNKMQLQR
                     SYHGNVMMLKRDAGAQDVVAQVIYKGDTFKQEMGETGRIKAIKHEQDFFNIDKENIIG
                     AYCTIVFNDGRDNYIEVMTIEQIKQAWMQSSMIKDEKALQNSKTHNNFKEEMAKKTVI
                     NRAAKRYINTSTDSNLFKYAQESEQRQRKEVLDAEVEENANQEQLDFEQPVLEEAQYT
                     ELENDKPIDVSDFEEIKEPATEKESEEEPF"
     gene            3886..4503
                     /locus_tag="P6L01_001942"
     CDS             3886..4503
                     /locus_tag="P6L01_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDP3030997.1"
                     /translation="MKFERVQKHFKYKTRHIAGCLITHEHGDHAKYTKQFVDNGVISY
                     MTAGTQQAMNFESHRLCTIKAKQELRIGTWSILPFDIEHDANEPVAFLLQSTLGYKVL
                     YVTDTKYLKYKFNGITHMMLEVNYIYEQMQENIKNGSVHSTLANRIMESHFSLEHAIG
                     MLKANDLTRLEEIHLIHLSSQNSNAKYIKSEIQKVTGAPVYFGGL"
     gene            4504..4974
                     /gene="ssb"
                     /locus_tag="P6L01_001943"
     CDS             4504..4974
                     /gene="ssb"
                     /locus_tag="P6L01_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDP3030998.1"
                     /translation="MLNRTILVGRLTRDPELRTTQSGVNVASFTLAVNRTFTNAQGER
                     EADFINIIVFKKQAENVNKYLSKGSLAGVDGRLQTRNYENKEGQRVYVTEVIADSIQF
                     LEPKNSNDTQQDLYQQQVQQTRGQSQYSNNKPVKDNPFANANGPIELNDDDLPF"
     gene            5004..5897
                     /locus_tag="P6L01_001944"
     CDS             5004..5897
                     /locus_tag="P6L01_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HDP3030999.1"
                     /translation="MTGWISIDRSIQNHWLFKEKRTFSKFEAWIYLLMEANHSEAKVP
                     IGNQIVTVERGQRLTSILTLSDLFNWSRFKVKTFLDLLESDGMLEVKTTSKYTLITIV
                     NYDFYQSEQGRNQHQNDINPTSKQHQSNINPTSKQHQTNTNNNDNKDNNEKNVNNEKK
                     KVTAFDFFQDNGFGFITPYNLDDLNYYLDSFENDSDEIVTASLKIAKDRNKVTWGYAK
                     SILNTWLNANLKSIEQVRAFEKQQLESKKQNYKPFVKQSKEKTPKWLTDSTRETKTPE
                     VDENLEKDREAFIKRLNSKWE"
     gene            5904..6122
                     /locus_tag="P6L01_001945"
     CDS             5904..6122
                     /locus_tag="P6L01_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031000.1"
                     /translation="MDAFDKYYLFDHDGNKMFSVTPHFKDGRHLVVGLKHTKFNGRRW
                     YLDDYELKTLIDNEQMELGHQTSLFEYI"
     gene            6225..6533
                     /locus_tag="P6L01_001946"
     CDS             6225..6533
                     /locus_tag="P6L01_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDP3031001.1"
                     /translation="MPTKYTEHKKYLQNQMPKLNLENALKIELEFYFTPPKSWSKKKK
                     TQAIGQLKVTKPDIDNLMKTVLDACNNYLWKDDNQIAEITSSKRYGIEPKIIIRIEEL
                     "
     gene            6546..>6871
                     /locus_tag="P6L01_001947"
     CDS             6546..>6871
                     /locus_tag="P6L01_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031002.1"
                     /translation="MARKARIVTINDKPYRFTKSEMELIESHGITAGMVSKRVKDGWE
                     LHEAMDAPEGTRLSEYREKKTIERLEQARLERKLERKRKKEAELRRKKPHLFNVPQKH
                     PRGRYA"
BASE COUNT         2870 a         1047 c         1230 g         1724 t
ORIGIN      
        1 aaaactgcta cttgcgccaa caagtaacag taatgtaatt agaaatataa acttacgttc
       61 aatataaaac gaaacaagga ggaagtcaac catgactaaa aactataaag acatgacgca
      121 ggaagaaata aaagacttat tatctgaaaa aacggcagaa ttgtatgaat tagcgaaaga
      181 aattaaggga gaaagtaaat ttgatatttt gcttttctca tcaataggag ttatcgacgg
      241 agattattta gcaggttcaa gttctgtgat tggtcatact tttgatcttg cttacttatt
      301 ggatagcact aagagttata aagatattgt caatgttctc caaatgtgta aatcacaaaa
      361 aattctcggt atagatgacg acaaggagga ctaaaacaat gtattacaaa acgggtgacg
      421 tatgtcgaaa aatatttaat gtagatggct ttgattttca attaagagtt aagaagcgag
      481 catatagtgt cgaaatagtc gttttagatc atgaaggaaa ttcaattgac gggctactag
      541 tttctgacga gaacgatcta tacacagctt tagatatttt gaaacaaagt atttatgaat
      601 ggattgaaaa taacacagat gaacaggaca gactaattaa cttagtcatg aaatggtagg
      661 tataagcatg agagatacag aaagaaatat attgaatatt tttaagacgt tattcgacga
      721 atatactttg tcaaaccaac gagcattatt ggaaattgaa cgtaatcatc acggatactt
      781 atcgattaat ttcttgcact atcacgacag ttacaaaaca aacaataagc ttgtgcagat
      841 acatgaaatc aatccagaca gccatgaacg aataaaaaat ttaattatcg aggtgctaag
      901 aggtcatcgg aagattaaaa aaggagcatg aggaaagata tgaaaataaa taagttaact
      961 atatcgaact ttgctggaat caaagaagaa aaatttaact ttgacggtaa agatgcaaaa
     1021 atatacggca ataatgcgac tggcaagact acaacagcaa ccgcattaca atggctgctt
     1081 ttcgataagg gtttagacgg ttcaaccaaa tcatttaacc ctgtaccttt aaacgaaaaa
     1141 aacgaagaaa attatgagtt aattccgact gttttcgcag aatttgaaat cgacggaaaa
     1201 attacgactt ttaaaaaaga gtcacatcct aaatacacaa taaatcaaaa aacgaatcgc
     1261 aaggaatact cacgaagtcg aacgaagaaa caatatatca atgatgaatc aataaaagta
     1321 aaggattata aagctcgtat tgatgaactg attgatgaag atgtattcaa gttaattacg
     1381 aaccctcaag catttaactt actagattgg aagaaacgaa gaagtttgtt gtttgaaatc
     1441 gctaaaccaa tcaatgatga ggatgtcatt aaaacaaatg atgattttaa agaactaaat
     1501 aatattcttg gagatcacga aattgaaaca aagaaaaaga ttcttacaga caagataaaa
     1561 cagattaaca aagatatcaa agatattccg atacgtatta accaaacgca acaaaataag
     1621 caggatgtac cggaattcga taatgataga cacacaatca taaaacaaga aattgagcaa
     1681 cttgaaaatg agcgtataga tattcaaaac ggtgcagaag aaattaattt gcgtaaccaa
     1741 ttagctgata aacaatcaga attgaagcgc atagaagcta ataatagcgc cagtaatgag
     1801 aacaaaatac atgctttaac aaatgagcta cacgttgaaa atggaacggt tgcgaatctt
     1861 aaaacaagat taaagcaaaa caaacaacaa attacacatg aagaaaatcg acgtaatcaa
     1921 ttattagaaa atcataaagg attaaaaagt gatttagaaa aagctaaaaa tcaaaaattt
     1981 gaatatcttg atgacaatgt atgtagttgt tgtggtcaac agttaccagc tgaacaagtg
     2041 agtgaggtaa gagaaaaagc attgcagaaa ttcaatgcaa acaaatcgaa agaattagaa
     2101 acaatacaaa catctatcaa tcacattatt tcagagggca agaaaataaa gccaattatc
     2161 gagaaattag aggatgacaa caataattta caaattaaaa tcaacgaagc agaagagcgt
     2221 tcagcaagaa tacaaaacaa aattaataag ttgaaaacaa ctcacgttga cgttacgcaa
     2281 actgacgaat acaaagcagt aatgttagag ataaatgaga ttaatcaaaa acgctctaac
     2341 atcaggaaaa ctattcaaga taaagtttca ggaatagatg acaaaataag cgaacttact
     2401 caagaaaaat cagaaattga agtgtcaata tcaatcgaaa aatcaaataa acatctagat
     2461 gatgttattt ctgaattaag aaatgaagaa gacagattat tggatgaaaa agaaaagtat
     2521 tcacatgacc tttatatctt aaaagaattt acaacaacaa aagtcaaaat gcttactgaa
     2581 aacatcaata acgaatttga tattgctgaa tttaagctat tcaatacctt agttaacggc
     2641 gaattagaag aaacatgttc aacaacggtt aatggtgtcg agtatgacag cggtttaaat
     2701 aacgcctcaa gaattaatgt tggcttagat atcatcaaca cactatcaaa acattttaaa
     2761 gttacagcgc caatatttat tgataatgct gaatcagtaa cagagcttat caaaacagaa
     2821 tcacaacaaa ttcaattgat agtaaatgaa caagataaaa aattaagaat ggagactata
     2881 taaaatgact gaaaataata aattacaaac tattgaacaa caattagtac aagaaaagaa
     2941 cgtatctgac aacgtattaa acaaagtgag agttttagag tcacaaggca atttggaatt
     3001 gccaaatgat tattcaccaa gtaatgccat gaaacaagca tggttacaaa tcagccaaga
     3061 taacaaatta atgagttgta acgatacaag caaagcaaat gccttattag acatggtaac
     3121 gcaaggttta aatccagcta aaaatcaatg ctactttatt ccttacggca acaaaatgca
     3181 gttacaacgt agctatcacg gtaatgtaat gatgttaaaa cgtgatgcag gtgctcaaga
     3241 tgttgttgct caagtgattt ataaaggcga tacattcaag caagaaatgg gagaaacagg
     3301 acgtatcaaa gcgattaaac acgaacaaga cttctttaac atcgacaaag aaaacattat
     3361 cggtgcgtac tgcacaatcg tatttaatga tggacgagat aactatattg aagtcatgac
     3421 tattgaacaa attaaacaag catggatgca gtcatcaatg attaaagatg aaaaagcatt
     3481 acaaaattct aaaacacata ataatttcaa agaagaaatg gctaaaaaaa cagttatcaa
     3541 tagagctgct aaacgttata tcaacacatc aacagatagc aatcttttca aatacgcaca
     3601 agaatccgaa caacgtcaac gcaaagaagt gttggacgca gaagttgaag aaaatgcaaa
     3661 tcaagaacaa ttggactttg aacaaccagt tcttgaagaa gcacaataca cagaattaga
     3721 aaatgataag cctattgatg tatctgactt tgaagaaata aaagaacctg caacagaaaa
     3781 agaaagcgaa gaagagccat tttaattgaa acaatagcaa ctggttcaag tggtaactgc
     3841 tacgtcttaa atgatggacg tactacgtta ctatttgagg caggtataaa atttgaacgt
     3901 gttcaaaagc atttcaaata taaaacaaga catatagcag ggtgtcttat cacacacgaa
     3961 catggtgatc atgcaaagta tacaaagcag tttgtcgaca atggtgtaat cagctatatg
     4021 actgctggaa cacaacaagc tatgaatttt gaaagtcatc gcttatgcac gattaaggca
     4081 aagcaagagc tgcgaatagg cacatggtca attctaccgt ttgacatcga acatgatgct
     4141 aacgagcctg tggctttctt attacaaagc acactaggtt ataaggttct gtatgttact
     4201 gatacaaagt atttgaaata caaatttaat ggcattacgc acatgatgct agaagttaat
     4261 tatatctatg aacaaatgca agaaaacata aaaaacggca gtgtgcacag cacattagca
     4321 aacagaatta tggagtctca ttttagctta gaacatgcta tcggaatgtt aaaagcaaat
     4381 gatttaacta gactcgaaga aatacattta attcatttaa gtagtcaaaa ttcaaatgca
     4441 aaatacatta aaagtgaaat acaaaaagtg acgggcgcgc ccgtttattt tggaggttta
     4501 taaatgctaa acagaacaat attagttggt cgtttaacta gagacccaga attaagaacc
     4561 actcaaagtg gtgtaaatgt agcatcattc acattagcag ttaaccgtac atttacaaat
     4621 gcacaaggcg agcgcgaggc agactttatt aatatcatcg tatttaaaaa acaagcagag
     4681 aacgttaata aatacctatc taaaggatcg ttggcgggcg tagatggtag gttacaaacg
     4741 cggaactatg aaaataagga aggtcaacgt gtatacgtta cggaagttat tgctgatagt
     4801 attcaatttt tagaaccgaa aaactcaaat gacactcaac aagatttata tcaacaacaa
     4861 gtacaacaaa cacgtggaca atcgcaatat tcaaataaca aaccagtaaa agataatccg
     4921 tttgcgaatg caaatggtcc gattgaacta aatgatgatg atttaccatt ctaatttaac
     4981 cggtttgaaa gtgaggtgtg tatatgactg gttggataag tattgatcgc tcaattcaaa
     5041 atcattggct atttaaagaa aagagaacat tttcaaagtt cgaagcatgg atatatttac
     5101 tcatggaagc gaatcattca gaggcaaaag tgcctatagg aaaccaaatt gtaaccgttg
     5161 aaagaggaca aagattaaca tctattttga ccttgtctga cctttttaac tggtcacgat
     5221 ttaaagtgaa aaccttcctt gacttactcg agagtgatgg aatgttagaa gtcaaaacaa
     5281 catcaaaata tacccttata accattgtca attatgactt ttatcaaagt gagcagggca
     5341 ggaaccaaca tcaaaacgac atcaacccaa catcaaaaca acatcagtca aacatcaacc
     5401 caacatcaaa acaacatcaa accaacacaa acaataatga taataaagat aataatgaaa
     5461 agaatgtgaa taatgagaag aagaaggtaa ccgccttcga cttcttccaa gataacggat
     5521 tcggtttcat aactccttac aatttagacg atttaaatta ctatcttgat tcatttgaaa
     5581 atgattcaga tgaaatagtt accgcatcac ttaaaatcgc taaagacaga aacaaggtta
     5641 cttggggata tgctaaaagc attttgaata cttggcttaa tgcaaacttg aaatctattg
     5701 aacaagtacg tgcatttgaa aagcaacaac ttgaaagcaa aaaacaaaat tataaacctt
     5761 tcgttaaaca atcaaaagaa aaaacaccca aatggctcac agacagcacg agagaaacga
     5821 aaacgccgga agtagatgaa aaccttgaga aagacagaga agcttttatt aagcgtctaa
     5881 atagcaaatg ggagtgattg aaaatggatg catttgataa atactatcta tttgatcatg
     5941 acggcaacaa aatgttttca gttacaccac attttaaaga tggacggcat ttagttgttg
     6001 gattaaaaca tacaaaattc aatggtcgac gttggtactt agatgattat gaattaaaaa
     6061 cacttattga taatgaacaa atggagttag gacaccaaac aagcttattt gaatatatat
     6121 gagggattac atggagatag aaattaaatt taacgaaact ttcgaggcac ctatgggctc
     6181 gcctcgaccg cgttttagca caaaaggtag atatgcacac atatatgcct acaaaatata
     6241 cagaacataa aaaatattta caaaatcaaa tgccaaagct aaatctagaa aatgcattaa
     6301 aaattgaatt agagttttac tttactccac ctaaatcatg gtcgaagaaa aagaaaactc
     6361 aagcgattgg acaattaaaa gtaactaaac cagatatcga taatttgatg aagacagttc
     6421 tagacgcttg taacaattat ttgtggaaag atgacaatca aattgcagaa ataactagct
     6481 caaagcgtta tggaattgag cccaaaataa tcatacgaat agaagaacta taagaggtgg
     6541 ataaaatggc gagaaaagca aggattgtaa caataaatga taaaccatat aggtttacca
     6601 aatctgaaat ggaattaata gaaagtcacg gtataaccgc tggaatggtt tctaagagag
     6661 taaaagacgg ttgggaacta catgaagcaa tggacgcacc agaaggtacg cgtttaagcg
     6721 agtacagaga aaagaaaaca atagaaagac tggaacaagc tagactcgaa cgcaaattgg
     6781 aaagaaagcg aaagaaagag gctgagctaa gaagaaagaa gccacatttg tttaatgtgc
     6841 ctcagaaaca tccaagagga cgttatgcgt g
//
LOCUS       DAOBBH010000131         6813 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.131, whole genome shotgun sequence.
ACCESSION   DAOBBH010000131 DAOBBH010000000
VERSION     DAOBBH010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6813
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.131"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(56..271)
                     /locus_tag="P6L01_001948"
     CDS             complement(56..271)
                     /locus_tag="P6L01_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HDP3031003.1"
                     /translation="MKVNKAIYVIVALFLGGLGIHKFYADKFGQGLFHLLFFWTGIPT
                     IISIIHAIFVIFTKKADKDGYIIFPKN"
     gene            436..987
                     /locus_tag="P6L01_001949"
     CDS             436..987
                     /locus_tag="P6L01_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDP3031004.1"
                     /translation="MIRQARPEDRFDIAKLVYMVWDDMELELVKHLPKDMVLDAIEKS
                     CVDATYRTFYQHILVYEVENKVAGCIISYSGENELKYEKAWELLDLPEEIKQYGTPLP
                     VKEAKDDEYYIETIATFAAYRGRGIATKLLTSLLESNTHVKWSLNCDINNEAALKLYK
                     KVGFISDGQIELYKHMYHHLIVK"
     gene            complement(1039..1977)
                     /locus_tag="P6L01_001950"
     CDS             complement(1039..1977)
                     /locus_tag="P6L01_001950"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDP3031005.1"
                     /translation="MSNQYQQYSTVKKYWHLMRPHTLTASVVPVLVGTAASKIYFLGS
                     EDHIKISLFIAMLLACLLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELV
                     LRLAIAFYILAAILGLFLAANSSFWLLPVGLVCMAVGYLYTGGPFPISWTPFGELFSG
                     VFMGMFIIVIAFFIQTGNIQSYVIWLSVPIVITIGLINMANNIRDRVKDKASGRKTLP
                     ILLGKNASLTFMAIMYFIAYAFIVLTIIIKPGGSLFYLLALLSFPMPVKVIRRFKKND
                     TPPTMMPAMAAAGKTNTFFGLLYALGIYISALFAGI"
     gene            2159..3520
                     /locus_tag="P6L01_001951"
     CDS             2159..3520
                     /locus_tag="P6L01_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HDP3031006.1"
                     /translation="MATGVLEDDIVKEIYGSSKEWVSVEVKLSQSLDPSTLFHLTDNE
                     AGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHL
                     RVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDL
                     MDLFNNISDIDVDEQIGEITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFD
                     KDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKR
                     SQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYH
                     LYTEIKAPLKDDSYISLIDHLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYID
                     IYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMN
                     GKS"
     gene            3507..5180
                     /gene="menD"
                     /locus_tag="P6L01_001952"
     CDS             3507..5180
                     /gene="menD"
                     /locus_tag="P6L01_001952"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDP3031007.1"
                     /translation="MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEA
                     HPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLI
                     VLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETINAIYYQMQIASQY
                     LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGL
                     IIVGDMQHQEVDQILTYSTIYDLPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVD
                     FVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISYEISANDFFRSLME
                     DTTINRVSWLEKWQCLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMP
                     IRDVDNLLLNKNIDVYANRGANGIDGIVSTALGMAVHKRITLLIGDLSFYHDMNGLLM
                     SKLNNIQMNIVLLNNDGGGIFSYLPQKESATDYFERLFGTPTGLDFEYTAKLYQFDFK
                     RFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL"
     gene            5167..5970
                     /gene="menH"
                     /locus_tag="P6L01_001953"
     CDS             5167..5970
                     /gene="menH"
                     /locus_tag="P6L01_001953"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDP3031008.1"
                     /translation="MTHYKYYEANVETNQVLVLLHGFLSDSRTYYNHIDKYTDICHVI
                     TIDLPGHGEDQSSMDETWNFDYITTLLDRILDKYKDKSITLFGYSMGGRVALYYAING
                     HIPISNLILESTSPGIKEEANQLERRLVDDARAKVLDIAGIELFVNDWEKLPLFQSQL
                     ELPVEIQHQIRLQRLSQSPQKMAKALRDYGTGQMPNLWPRLKEIKVPTLILAGEYDEK
                     FVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND"
     gene            5963..6784
                     /gene="menB"
                     /locus_tag="P6L01_001954"
     CDS             5963..6784
                     /gene="menB"
                     /locus_tag="P6L01_001954"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDP3031009.1"
                     /translation="MTNRQWETLREYDEIKYEFYEGIAKVTINRPEVRNAFTPKTVAE
                     MIDAFSRARDDQNVSVIVLTGEGDLAFCSGGDQKKRGHGGYVGEDQIPRLNVLDLQRL
                     IRIIPKPVIAMVKGYAVGGGNVLNVVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLA
                     RIVGHKKAREIWYLCRQYNAQEALDMGLVNTVVPLDKVEDETVQWCKEIMKHSPTALR
                     FLKAAMNADTDGLAGLQQMAGDATLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP"
BASE COUNT         2470 a          991 c         1265 g         2087 t
ORIGIN      
        1 atcttacgaa ctatataccc attcatatat gctttttaaa cgtcattgtc acaatttaat
       61 ttttagggaa tataatataa ccatctttat ctgctttttt agtaaaaatg acaaaaattg
      121 catgtattat tgagatgatg gtagggatac ctgtccagaa aaataataag tgaaaaagac
      181 cttgtccaaa tttatcagca taaaatttat gaatacctaa acctccaaga aataatgcaa
      241 caataacata aatggcttta ttgactttca tttgtaaatc ctccttaact ataattctac
      301 ttaaattcgt tgtgaaaacc aatatttcta actttagaat tttcaaactt tctaaaatta
      361 taagtatatc tttttaaaat aagctagaat ttctatataa taaatgttaa taacgtaaaa
      421 gggaatgatg acatagtgat acgtcaagca cgtccagagg accgatttga tattgcgaag
      481 ttagtttata tggtttggga tgatatggaa ttagaattgg taaagcatct acctaaagac
      541 atggtattag atgcaattga aaaaagctgt gttgatgcaa catatcgaac tttttatcag
      601 catattttag tttatgaagt agaaaataaa gtagcaggtt gtattattag ctatagtggt
      661 gaaaatgaat tgaaatacga aaaagcatgg gaactacttg acttgccaga agaaataaaa
      721 caatatggca cgccattacc tgtaaaagaa gctaaagacg atgagtatta tatagaaaca
      781 attgcgacat ttgcagcata tagaggtaga ggcatcgcga caaagttatt aacgtcatta
      841 cttgaatcaa atacacatgt taaatggagt ttgaattgcg atattaataa tgaagcagca
      901 ttaaagttat ataaaaaagt aggctttata tctgatggac agattgaatt atacaagcac
      961 atgtatcatc atttaattgt taaataaaaa aactcgacag ttcgatgtaa gtcgattgcc
     1021 gagtagtatc attttctatt aaatgcctgc aaataatgca ctaatataaa tacctaatgc
     1081 atataataaa ccgaaaaatg tatttgtttt accagcagca gccattgctg gcatcattgt
     1141 aggcggtgta tcattcttct tgaaacgtct gataacttta acaggcattg ggaatgataa
     1201 caacgcaagt aagtaaaata atgagccacc aggtttaata atgatcgtaa gtacaataaa
     1261 ggcataagcg ataaagtaca tgattgccat aaatgttaaa gaagcatttt tacctaatag
     1321 aatgggtaaa gttttgcgac cacttgcttt atctttgaca cggtcgcgaa tattgttagc
     1381 catattaatt aaaccgatag tgattactat aggtacactt aaccaaatta cataactttg
     1441 aatattgcca gtttgaataa agaatgcaat aacgataata aacataccca taaatacgcc
     1501 tgagaataat tcaccgaaag gcgtccatga aatagggaaa gggccacctg tatataggta
     1561 accaacagcc atacatacta atccaactgg taataaccaa aatgaagagt tagcagctaa
     1621 aaacaaacct aatattgctg ctaagatgta aaatgcaatg gctaatcgta gcacaagctc
     1681 tgggctcata ccgttgcgaa caatggcacc accaatgcct acagattcat gatcatcaag
     1741 gcctttttta taatcatagt attcattaaa catattagtt gctgcttgaa taagtaagca
     1801 tgctagtaac atggcaatga ataggctgat tttaatatga tcttcgctac caagaaaata
     1861 tattttagat gctgctgtac caactaaaac gggtactacg gaagcagtta atgtatgagg
     1921 acgcattaaa tgccaatatt tcttaactgt agaatattgc tgatattgat tactcattga
     1981 atacaaaacc tctttaaatc atatttttta taacaaagat attttaatag aaagtagcat
     2041 aaaggtcaat tgacgtgaaa aattgcaaga taagtttaag caattcttgt aacacaatta
     2101 tccaaaaatg atacaatata ataatgagtg aaaaaagacg aagaaagaag tgaaatggat
     2161 ggctacgggc gtattagagg acgatattgt caaagagata tatggcagct caaaggaatg
     2221 ggtttcagtt gaagtgaaat tatcacagtc actagacccg agcacattat ttcatctcac
     2281 tgacaatgag gcaggagatc gcttttatat gcgtttgaat gataatcgaa cgtcatattt
     2341 tggctacaaa gcaattcaat tattcaaaaa taattctaaa aataaacaat ctatttttaa
     2401 agactgggaa aaattaaaac ataacatcac atttatacat ccgcaatctg agaaacatca
     2461 tcttcgagtt gttggagggt ttcaattttc aagtcataaa tcagatgatg aatggcgaga
     2521 gtttggacta aatcattttg tattacctga agttttaatt tcaactgata ataatgggac
     2581 atttttaact tatacagtta aaagggaaag ttttactgtt gaggcattga acgatttaat
     2641 ggatttgttc aacaatatat cggatataga tgtggacgag caaattgggg aaattactag
     2701 aaatgaagat atttataaag atgactggcg tcaacttgta gtagaagcta tagaatctat
     2761 taataatgaa gaaaaaattg tactagcacg tagacggtta ataaagttcg ataaagatat
     2821 cagtattcca tatattctaa agcaagcata ttctaaagaa aaaaacagtt atatattctt
     2881 gttagaatca caagattcta ttttcttttc acaaacacct gaacaattaa taaaggtcaa
     2941 taataaaata ctatcgacta aagctgtagc aggtacaatt aaacgttcac aagatgagga
     3001 cgaagataca aaaaatgttg aagcattttt aaaagataat aaaaacttaa tcgaacatcg
     3061 atttgttgtt gacagtattt tacatgatat taaaccttat atcactgaat tacattatga
     3121 taagacgcct aaaattctaa aaaatgatca tttatatcac ttgtacactg aaataaaggc
     3181 gccactgaag gatgattcgt atattagttt aattgatcat ttacatccaa cacctgcttt
     3241 aggtggctat ccaaaagaat ttgcgatgga ttttattgaa cagaaagaat ttggtacacg
     3301 aggattatat ggtgcgccgg ttggctatat agatatatat gatgattgtg aatttattgt
     3361 tgcaattcgt tcgatgctta ttaagaaagc acaagcaact ttatttgctg ggtgtggcat
     3421 tgttaaagat tctgatccag atagtgaatt ggcagaaacg aaccttaagt tcacacctat
     3481 gatgaatgca ttaggagtcg atatgaatgg gaaatcataa agcagcttta acgaagcaag
     3541 tttttacatt tgcatctgag ttatatgcat acggcgtaag ggaagtagtt atcagtccgg
     3601 gatcacgctc aacgccactt gcacttgcat ttgaagcaca tccaaatatt aaaacatgga
     3661 tacaccccga tgagcgaagt gcagcatttt ttgcagttgg gttaattaaa ggcagtgaaa
     3721 gacctgtcgc tatattatgt acgtcaggta cagcagcagc gaattatacg cctgcaattg
     3781 ctgaaagcca aattagtaga attccattaa tcgttttaac aagtgaccgt ccgcatgaat
     3841 taagaagtgt aggcgcacca caagcgatta atcaagtaaa tatgtttaat aattatgtaa
     3901 gttatgagtt cgatatgcct attgcggatg atagtaaaga gaccattaat gcaatttatt
     3961 atcaaatgca aattgctagt caatatttat atggaccaca taaagggcca attcatttta
     4021 acttgccatt tagagatccg ttaacacctg atttgaatgc aacagaattg ttaacttctg
     4081 agatgaagat tttaccgcac tatcaaaaaa gtatagatgc atcggcatta agacacattt
     4141 taaataagaa aaaaggttta attattgtag gggatatgca gcaccaagaa gttgatcaaa
     4201 tactaacgta ttcaacgata tatgatttgc ctattttagc tgatccttta agtcatttaa
     4261 gaaaatttga tcatccgaat gttatctgta catatgattt gctgtttaga agcggcttag
     4321 acttaaatgt ggatttcgta attcgtgttg ggaaaccagt gatttctaaa aagttgaatc
     4381 aatggttaaa gaaaactgat gcatttcaaa tattagtgca aaacaatgat aagattgatg
     4441 tctttcctat agcgccagat atttcatatg agatttctgc aaatgatttc tttagatcat
     4501 taatggaaga cacgaccatc aatcgcgtaa gttggttaga aaaatggcaa tgcttagaga
     4561 aaaaagggcg taaagaaatt aaatgttatt tggaacaagc tacagatgag agtgcattcg
     4621 ttggtgaatt gattaagaaa acatctgaaa aagatgcatt atttattagt aatagtatgc
     4681 ctatcagaga tgtagataac ttgttattga ataaaaatat agatgtctat gcgaatcgtg
     4741 gtgcgaatgg tattgatggt atcgtttcaa ctgcactggg tatggctgtg cataaacgaa
     4801 taacattatt gataggtgat ttgtcatttt atcatgatat gaatggacta ttaatgtcaa
     4861 aattaaataa tattcagatg aatattgtat tattgaacaa cgatggtggc ggtatttttt
     4921 catatttacc acaaaaagaa agtgcaactg actattttga acggttgttt ggcacaccga
     4981 cgggattgga tttcgagtat acagctaagt tatatcaatt cgattttaaa cgttttaaca
     5041 gtgtttcaga atttaaaaat gccacattgt tatctgaaac ttcgacgatt tatgaattga
     5101 taacgaatcg cgaagataac tttaaacagc atcaaatttt atatcagaaa ttgagtgaaa
     5161 tgattcatga cacattataa atactatgaa gcaaacgttg agaccaatca agttttagta
     5221 ttactgcatg gttttcttag cgacagtcgt acttattata atcacatcga taaatatact
     5281 gatatctgtc atgtcatcac tatagactta ccaggccatg gcgaagatca gtcttcaatg
     5341 gatgaaacgt ggaattttga ttatattacg acgttgttag accgaatttt agataaatat
     5401 aaagataaat caataacatt gtttggatat tcaatgggtg ggcgtgttgc attatattat
     5461 gcaattaatg gtcacatccc tatatctaat ttgatattag aaagtacgtc accaggtatt
     5521 aaagaagaag caaatcaatt ggaacgccgt cttgttgatg atgcacgtgc taaagtatta
     5581 gacatagcag gtattgaatt atttgttaat gattgggaaa agttgccatt atttcaatcg
     5641 caactagaat taccagttga aatacaacat caaataagac tacaacgatt gtctcaatcg
     5701 ccacagaaaa tggccaaagc attaagagat tatggtacag gtcaaatgcc aaacttatgg
     5761 ccgcgcctga aagaaattaa agtaccaaca ttaatattag ctggagaata tgatgaaaaa
     5821 tttgtacaga ttgcgaaaaa aatggcaaat ttaattccta atagtaaatg taaattaatt
     5881 tctgctacag gtcatacaat tcatgtggaa gatagtgatg aatttgatac aatgatatta
     5941 ggatttttaa aggaggagca aaatgactaa cagacaatgg gaaacactta gagaatatga
     6001 tgaaatcaaa tatgaatttt acgaagggat tgctaaggta acaataaatc gccctgaagt
     6061 acgcaatgcg tttacaccta aaacagttgc tgaaatgatt gacgcatttt cacgtgcacg
     6121 tgatgatcaa aacgtttcag ttatcgtatt aactggtgaa ggtgatttag cattctgttc
     6181 tggtggtgac cagaagaaac gtggacatgg tggttatgta ggtgaagacc aaatccctcg
     6241 cttaaatgta ttagatttac agcgtttaat tcgtattatt ccaaaaccgg ttatcgcgat
     6301 ggtaaaaggt tatgctgtag gtggcggtaa tgtactaaat gttgtttgtg acttaacgat
     6361 tgctgctgat aatgctattt ttggacaaac tggtcctaaa gtaggttcat ttgatgcagg
     6421 ttatggttca ggatatttag cacgtatcgt tggacataag aaagcacgtg aaatctggta
     6481 cttatgtcgt caatacaatg cacaagaagc tttagatatg ggcctagtaa atacagtggt
     6541 acctttagat aaagttgaag atgaaactgt gcaatggtgt aaagagatta tgaaacactc
     6601 accaacagct ttacgattcc ttaaagcagc tatgaatgct gacacagatg gtttagctgg
     6661 tttacaacaa atggctgggg atgcaacatt gctttattac acaactgatg aagcgaaaga
     6721 aggccgtgat gcgtttaaag aaaaacgtga tcctgacttc gatcaattcc ctaaattccc
     6781 ataagttatt ttgaatatga tattaagtca ctt
//
LOCUS       DAOBBH010000132         6771 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.132, whole genome shotgun sequence.
ACCESSION   DAOBBH010000132 DAOBBH010000000
VERSION     DAOBBH010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.132"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(55..128)
                     /locus_tag="P6L01_001955"
     tRNA            complement(55..128)
                     /locus_tag="P6L01_001955"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(94..96),aa:Gly,seq:tcc)
     gene            complement(150..232)
                     /locus_tag="P6L01_001956"
     tRNA            complement(150..232)
                     /locus_tag="P6L01_001956"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(197..199),aa:Leu,seq:gag)
     gene            complement(879..1334)
                     /locus_tag="P6L01_001957"
     CDS             complement(879..1334)
                     /locus_tag="P6L01_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDP3031010.1"
                     /translation="MNNDKLQRMVENLSEEKFGRTFRHCAYFNKRLRTTGGRYLLKSH
                     DIEINPKQYEHYGEDAVVKIILHELCHYHLHIAGKGYQHKDQDFKRLSQQVGAPRFCN
                     SIESYQQRANYEYYCTKCHAKYIRIRKVDTNRMRCGHCNGKLRMKRQLK"
     gene            complement(1327..3477)
                     /locus_tag="P6L01_001958"
     CDS             complement(1327..3477)
                     /locus_tag="P6L01_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDP3031011.1"
                     /translation="MDNQLINSIIEKYQFSKKQIEAVLTLLEEKNTVPFIARYRKEQT
                     GGLDEVQIKQIDDEYQYMVNLQKRKEEVIKNIEQQGLLTEELKKDILKQNKLQRVEDL
                     YRPFKQKKKTRATEAKRKGLEPLAIWMKARKHEVSIEEKAQQFINEEVQSVEDAIKGA
                     QDIIAEQISDNPKYRTKILKDMYHQSVLTTSKKKNAEDEKGIFEMYYAYSEPIKRIAN
                     HRVLAVNRGEKEKVLSVKFEFDTTSVEDFIARQEINHNNVNRSYILEAIKDSLKRLIV
                     PSIEREIHADLTEKAENHAIDVFSENLRNLLLQPPMKGKQILGVDPAFRTGCKLAVIN
                     PFGTFIAKGVIYPHPPVSKKEAAEKDFVQMVKAYDVQLIAIGNGTASRETEQFVADLI
                     KKHQLPVQFIIVNEAGASVYSASEIARDEFPDFQVEERSAVSIGRRVQDPLSELVKID
                     PKSIGVGQYQHDVNQKALENALTFVVETAVNQVGVDVNTASSSLLQYVSGLSSQIAKN
                     IIAYREENGAIKHNKELSKIKRLGAKTFEQSIGFLRIVDGSEPLDNTSIHPESYKVTY
                     QLLDKLGFGGNDLGSDALKAKLNSLDMDELAIELQVGVPTLEDIIKSLKAPNRDPRDE
                     FDTPILKSDVLSIEDLKEGMKLSGTVRNVVDFGAFVDIGVKQDGLVHVSKLSKKFVKN
                     PMDIVSVGDIVDVWVYSIDKNKDKVSLTMIDPHE"
     gene            complement(3911..4681)
                     /gene="sigB"
                     /locus_tag="P6L01_001959"
     CDS             complement(3911..4681)
                     /gene="sigB"
                     /locus_tag="P6L01_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigB"
                     /protein_id="HDP3031012.1"
                     /translation="MAKESKSANEVSPEQINQWIKEHQENKNTDAQDKLVKHYQKLIE
                     SLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFERKFEAFLVPTVIGEIKRYLRDK
                     TWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIANRLEVSEEEVLEAMEMGQSY
                     NALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCT
                     FIEGLSQKETGERIGLSQMHVSRLQRTAIKKLQEAAHK"
     gene            complement(4656..5135)
                     /gene="rsbW"
                     /locus_tag="P6L01_001960"
     CDS             complement(4656..5135)
                     /gene="rsbW"
                     /locus_tag="P6L01_001960"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma B factor RsbW"
                     /protein_id="HDP3031013.1"
                     /translation="MQSKEDFIEMRVPASAEYVSLIRLTLSGVFSRAGATYDDIEDAK
                     IAVSEAVTNAVKHAYKENNNVGIINIYFEILEDKIKIVISDKGDSFDYETTKSKIGPY
                     DKDENIDFLREGGLGLFLIESLMDEVTVYKESGVTISMTKYIKKEQVRNNGERVEIS"
     gene            complement(5137..5463)
                     /locus_tag="P6L01_001961"
     CDS             complement(5137..5463)
                     /locus_tag="P6L01_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HDP3031014.1"
                     /translation="MNLNIETTTQDKFYEVKVGGELDVYTVPELEEVLTPMRQDGTRD
                     IYVNLENVSYMDSTGLGLFVGTLKALNQNDKELYILGVSDRIGRLFEITGLKDLMHVN
                     EGTEVE"
     gene            complement(5582..6583)
                     /locus_tag="P6L01_001962"
     CDS             complement(5582..6583)
                     /locus_tag="P6L01_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HDP3031015.1"
                     /translation="MEEFKQHYKGLIDESLTCQDKVELIKKCEKYTDEVIRKDVLPED
                     IVDIHKNYILTLNLTREDVFKTLDVLQEIVKGFGYSYRDYQRLVDKLQVHDKEIDLAS
                     SLQQAMLKTDIPQFDSIQIGVISVAAQKVSGDYFNLIDHNDGTMSFAVADVIGKGIPA
                     ALAMSMIKFGMDSYGHSQLPSDGLKRLNRVVEKNINQNMFVTMFYGLYEEMNHLLYCS
                     SAGHEPGYIYRAEKEEFEEISVRGRVLGISSQTRYQQQEIPIYLDDLIIILTDGVTEA
                     RNSEGTFIDKQKLLEYIKKHKHMHPQDIVQIIYEAILKLQNPNKKDDMTILIIKRVN"
BASE COUNT         2053 a         1294 c          856 g         2568 t
ORIGIN      
        1 gaattaacgt tgacatattg tcattcagtt ttcaatgttc attaaaatta tctatggagc
       61 gggtgatggg aatcgaaccc acaacatcag cttggaaggc tgaggttttg ccattaaact
      121 acacccgctt atttaattaa gaattttgat gcggccgaga ggacttgaac ctccacggga
      181 tttctcccac taggccctca acctagcgcg tctgccattc cgccacgacc gcggatatag
      241 gcatttaaaa catcttcgtt tttcagaacg ttatttatta tattatgttt taaaatattt
      301 gtcaataact tttaatcgtt tttttacaat aaattaattt gtgttatcag ttgttaacac
      361 aacgctattt ataataaaac attaagaact tattgtcaac atattttata aattaaattt
      421 agaattgact gtctttcaaa tcaaagttta tttaatttat tcttttttaa ggaacgtgtt
      481 taatcatact aactataagc tgtacagtca atagaaaagt tcaatcaaaa tgtcattaaa
      541 aatgtataat tatcttttgc gctactttaa agctttaatt gggtaaagtt tatatattat
      601 ttattactaa aatacgtcaa gatttttaat ctaatcaatt taatttcatc ctatcaagtc
      661 acaatttatg ccattaccta tatcataagt agaaaagccg agattgcgaa gtattaaatt
      721 acgacaatct cggctttaaa cattttataa acacaatttc ttcttattca attactatgt
      781 ttaccaatca aaataactca ctgataaatt ctatagaaat tccatacaac tcataaataa
      841 tcttacatat acacgaatta gctctatttg tattgtactt atttcaattg tcttttcatt
      901 cttaattttc cattgcaatg tccgcaacgc atacgattcg tatcaacttt acggatacgt
      961 atatattttg catgacattt agtacaataa tattcataat tcgcccgttg ttgatagctt
     1021 tcaatgctgt tacaaaatct aggcgctcca acctgttggc tcaatcgttt aaaatcttga
     1081 tctttatgtt gataaccttt accagcaata tgcaagtgat aatgacacaa ttcgtgcagt
     1141 ataattttta caacagcatc ttctccataa tgctcatatt gttttggatt aatttcaata
     1201 tcatgggact ttaaaagata acgtccgcct gttgtacgta accttttatt aaaatatgca
     1261 caatgtcgaa acgtacgtcc aaatttttct tccgaaaggt tctcaaccat tcgctgaagt
     1321 ttgtcattat tcatgtggat caatcatcgt taatgatact ttgtctttat ttttgtcaat
     1381 actgtaaacc caaacgtcaa cgatatcacc aacactgaca atatccattg gattttttac
     1441 gaacttctta gaaagtttcg aaacatggac aagtccatct tgtttaacac ctatatctac
     1501 aaatgctcca aaatctacta catttctaac tgtgccactc aatttcattc cttcttttaa
     1561 atcttcaatt gatagcacat cggatttaag gattggtgta tcaaactcgt cccttgggtc
     1621 tcgattaggt gctttcaagg atttaataat atcctctaat gtaggtacac cgacttgtaa
     1681 ttcaatcgcc agttcatcca tatccaatga atttaattta gcttttaatg catcactacc
     1741 taaatcattg ccaccaaatc cgagtttatc taataactga tacgttactt tatagctttc
     1801 gggatgaatc gatgtattat ccaatggctc tgaaccgtct actattctta aaaatcctat
     1861 actctgctca aaagttttag cacccaaacg cttaatcttg cttaactctt tattatgctt
     1921 tattgcgcca ttttcttctc tatatgcaat tatattttta gcaatttgcg aacttagacc
     1981 agacacatat tgtaataatg atgatgatgc tgtattgaca tcaacaccta cttggttaac
     2041 tgctgtttca acaacgaaag ttaatgcatt ttctagtgct ttttgattaa catcatgctg
     2101 gtattgtccg acaccaatag atttaggatc gattttaact aattcactta atggatcttg
     2161 aacgcgtcgt ccaattgata cggcacttct ttcttccact tgaaaatcag gaaattcatc
     2221 tctagcaatt tctgatgctg agtatactga agcgcccgct tcattgacaa tgatgaattg
     2281 tactggcaac tgatgctttt taattaaatc tgcaacaaat tgttctgttt cacgacttgc
     2341 agtaccattg ccaattgcaa ttaattgcac atcatacgct ttaaccattt gtacaaaatc
     2401 cttctctgct gcctcttttt tagaaactgg tggatgcgga taaatcacac cttttgctat
     2461 aaaagtaccg aatgggttaa tgactgctaa tttacaacct gttctaaatg ctggatctac
     2521 gcctaatatt tgtttacctt tcattggagg ttgcagtaat agatttctta agttttcact
     2581 aaaaacatct attgcatgat tttcagcttt ttcagttaaa tcagcatgga tttcacgctc
     2641 tatcgaaggg acaattaagc gtttcaagct atctttaatc gcctctaaaa tataactgcg
     2701 atttacatta ttatgattga tttcttgacg tgcaatgaaa tcctctactg atgtcgtatc
     2761 gaattcaaac tttacagata atactttctc tttttcacca cgattaacag ctaaaactct
     2821 atgattagca atgcgtttaa ttggctcact atatgcatag tacatttcaa aaataccttt
     2881 ttcatcttca gcatttttct ttttagatgt agttaacaca ctttgatgat acatatcttt
     2941 taaaattttt gttctatatt taggattatc tgaaatttgt tccgcaataa tatcttgtgc
     3001 acctttgata gcatcttcaa ccgattgcac ttcttcattt ataaattgtt gtgctttttc
     3061 ttcaattgag acttcatgtt tacgtgcctt catccatatc gctaatggct ctaacccttt
     3121 acgtttcgcc tcagttgccc ttgtcttttt cttttgttta aaaggcctat ataggtcttc
     3181 aacacgttgt aatttgttct gttttaaaat atccttcttt aattcctcag taagtaatcc
     3241 ttgctgttct atatttttga taacttcttc tttacgtttt tgtaaattga ccatatattg
     3301 gtattcgtca tcaatttgct ttatttgaac ttcatctagt ccaccagttt gctcttttcg
     3361 atacctcgca ataaatggta ctgtattttt ttcttctagc agtgttaata ctgcttcaat
     3421 ttgtttttta ctaaattgat atttctctat gattgaatta atcaattgat tgtccattta
     3481 cttaacccac actttttctt aaatttactc atttatttta acatattcta aaatacttct
     3541 attaaggtat gatacttaat gtaattttca cttccaaaac atttaaaacg aattattagg
     3601 tcatactata ttttaaaacg cactaagatt attgaaaatg acacaccatc atttctaaat
     3661 ttcataatct aagactactt tttttattct aatagcaatc cacctattta aaacgacaat
     3721 tagatatctt cttcacttaa attattaata ctatatcgcg acatttatgt ggatacacat
     3781 taaactacgt taataatgta ttaattcgaa aatcttaatt aaattaaaaa tacccctcga
     3841 tttcaattta ataatcaagg gataattata tggatttttc attataaaac gtatcattaa
     3901 taagcaaatt ctatttatgt gctgcttctt gtaatttctt aattgccgtt ctctgaagtc
     3961 gtgatacatg catttgactt aaaccgatac gctcacctgt ctctttttga ctcaatcctt
     4021 caataaacgt acattgtatg atttctcgtt cgcgatcaga taatataggt aatatttttt
     4081 ctaaaatcat acgtttttca gttaagtcat aatggtcatc ttgttgcccc ataatatcta
     4141 atagcgtaac agttgaacca tctttatcag cttcaatgga atgatcaaca cttaacgcat
     4201 tataactttg tcccatttcc attgcttcta acacttcttc ttctgagact tctaatcgat
     4261 tagctatttc actgatagaa ggtgaacgct ctaattcagc ggttagttca tcgctcactt
     4321 ttttgattct tggcccaatt tctttaatac gtctcggaac atgtacactc caagttttat
     4381 ctcgtagata tcttttgatt tcaccgatta cagtaggtac taaaaaggct tcaaacttcc
     4441 gttcaaagga catatcgaat ctatttatgg cacctattaa accaaccata ccaacttgaa
     4501 ctaaatcttc gtgatgtgat tgtcctttag aatatttata tgccaatgac tcaattagtt
     4561 tttggtaatg tttaactaac ttatcctgtg catctgtatt cttattttct tggtgttctt
     4621 taatccattg gttaatttgc tcaggtgaaa cttcattagc tgatttcgac tctttcgcca
     4681 ttatttcgca cctgctcttt ttttatatac ttagtcatac tgattgtcac accagattct
     4741 ttatatactg tgacttcatc cattaaagat tcgattaaaa ataaacctag gccaccttcg
     4801 cgtaaaaagt ctatattttc gtctttatcg taaggaccta tttttgattt agttgtttca
     4861 taatcaaaac tgtcaccttt atcagaaata acaattttaa ttttatcttc taaaatttca
     4921 aaatatatgt taataatgcc cacattgtta ttttctttgt atgcatgttt aactgcattt
     4981 gtcacagctt cactaactgc aatcttggca tcttcaatat catcatatgt agcaccagct
     5041 ctcgaaaaaa cgccagaaag tgttaaacga attaaactta catactctgc cgatgctggc
     5101 acgcgcattt cgataaaatc ttctttagat tgcatgttat tcgacctccg ttccttcatt
     5161 aacatgcatt aaatccttaa gaccagtaat ttcaaatagt ctaccgatac gatctgacac
     5221 acctaaaatg tatagttctt tatcattttg gtttaatgct tttaatgtac ctacgaataa
     5281 acctaaacct gtcgaatcca tataactcac attttctaaa ttaacataaa tatcacgagt
     5341 tccatcttgt ctcataggtg ttaaaacctc ttctaattca ggcacagtat aaacatctaa
     5401 ttctccaccg actttaactt cgtaaaattt atcttgagtg gttgtttcta tattaagatt
     5461 catttcatta cactcctact tattaaaaat atttatcttc attttaagcg ctgtatccac
     5521 catatttata tttcaaatta ttatataccc atcgaaataa tttctaatct tcttttttaa
     5581 attaatttac tctttttata atcaaaatag tcatatcatc ttttttattt gggttttgaa
     5641 gctttaaaat tgcttcatag ataatttgaa caatatcttg tgggtgcata tgtttatgtt
     5701 ttttaatata ttctaaaagt ttttgtttat ctataaaggt accttcacta tttctagctt
     5761 cagtcacacc atccgttaaa atgataatta aatcatcaag gtatatagga atttcttgtt
     5821 gttgatatcg tgtttgtgaa ctgattccta acactctacc tctaactgaa atttcttcaa
     5881 attcttcttt ttcagcgcga taaatatatc caggctcatg accagctgaa ctacaataca
     5941 ataaatggtt catttcttca tataaaccat aaaacattgt gacgaacata ttttgattaa
     6001 tattcttttc aacaacacga tttaaacgtt ttaagccatc actcggtaat tgtgagtgtc
     6061 cataagaatc catgccaaac tttatcatac tcattgctaa agcagctggt atacctttcc
     6121 caatgacatc tgcaacagca aagctcattg tgccatcgtt atggtcaatt aaattaaaat
     6181 aatctccact tactttttgt gctgccactg aaataacgcc aatttgaata ctatcaaatt
     6241 gtggaatatc tgttttaagc attgcttgtt gtaagctaga agctaagtct atctctttat
     6301 cgtgaacttg aagtttatct accaatcttt gataatctcg ataactataa ccaaagcctt
     6361 taacgatttc ttgtaagaca tctaatgtct tgaacacatc ttcacgcgtt aagtttaacg
     6421 tcaatatata gtttttgtga atatcgacaa tgtcttcagg caagacgtcc ttacgaatca
     6481 cttcgtcagt gtatttctca cactttttta tcaattctac tttatcttgg cacgttaaac
     6541 tttcatcaat taaaccctta taatgttgct taaattcttc cacgattagt tgcctcctca
     6601 cttcccttac aaagcaaatg cttaagcaag gataattatg attcacttac taacatttat
     6661 ttaattgcac ttatcaattt tgtcgtttgc aattttgctg tagatgatac ttttaagcta
     6721 aaaaaaactt taggatggaa tacctaaagc ctaaaaaact taagacatcc t
//
LOCUS       DAOBBH010000133         6716 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.133, whole genome shotgun sequence.
ACCESSION   DAOBBH010000133 DAOBBH010000000
VERSION     DAOBBH010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6716
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.133"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(115..495)
                     /gene="blaI"
                     /locus_tag="P6L01_001963"
     CDS             complement(115..495)
                     /gene="blaI"
                     /locus_tag="P6L01_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HDP3031016.1"
                     /translation="MTNKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            complement(485..2241)
                     /gene="blaR1"
                     /locus_tag="P6L01_001964"
                     /pseudo
     CDS             complement(485..2241)
                     /gene="blaR1"
                     /locus_tag="P6L01_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
     gene            2348..3193
                     /gene="blaZ"
                     /locus_tag="P6L01_001965"
     CDS             2348..3193
                     /gene="blaZ"
                     /locus_tag="P6L01_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HDP3031017.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALN
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            complement(3719..4138)
                     /locus_tag="P6L01_001966"
     CDS             complement(3719..4138)
                     /locus_tag="P6L01_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDP3031018.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLIN
                     SNLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWP
                     TLEKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
     gene            4262..5233
                     /locus_tag="P6L01_001967"
     CDS             4262..5233
                     /locus_tag="P6L01_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDP3031019.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKD
                     MLALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEY
                     AQVPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGG
                     VGSVAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLD
                     TVGSNVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIR
                     EDIWSKLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
     gene            complement(5405..5497)
                     /locus_tag="P6L01_001968"
     CDS             complement(5405..5497)
                     /locus_tag="P6L01_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDP3031020.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            complement(5654..6001)
                     /locus_tag="P6L01_001969"
     CDS             complement(5654..6001)
                     /locus_tag="P6L01_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDP3031021.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKK
                     LKIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDD
                     EIREFFSKNHEGF"
     gene            complement(6020..6637)
                     /gene="cadD"
                     /locus_tag="P6L01_001970"
     CDS             complement(6020..6637)
                     /gene="cadD"
                     /locus_tag="P6L01_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter CadD"
                     /protein_id="HDP3031022.1"
                     /translation="MIQTVVAAAVLYIATAVDLLVILLIFFARAKTRKEYRDIYVGQY
                     LGSIILILVSLFLAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKKELNE
                     KGLSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLLTLFVFLILIFFLVFTAQK
                     LANIPGIGEIVEKFSRWIMAIIYIALGLFIIIENDTIQTILGFIF"
BASE COUNT         2444 a          944 c          873 g         2455 t
ORIGIN      
        1 tattttctgg acactctcat ctttaataat atattttaaa tgtcattaac aaaactaatt
       61 taataagagt caagtatggt ttacatatgt attatgtttg aaacatacct ctaattactt
      121 tttactaata tcatttaaaa tgtctcgcaa ttcttcaatt tctttgttat ttaattcttc
      181 atttttcgca aaattcagca ctaaactttt catatcccct ccatacagtt tattaagaaa
      241 ggttttagca gttttcattt taatatcgtc ttctttaata tttgatgagt aaaaataaat
      301 attttctgat ttgtatcgtt ttataatctc ttttttatat agtcttgtga ttaatgttct
      361 aatcgtttta tcactaactt ctttatattt ttgaatttca actacaattt cattagctga
      421 tactgatttt ttatcccata ttatattcat aacatcccat tcagccatag atatttcaac
      481 ttgcttattg gtcatttaaa acacccattc cttttaatat tttttcactt attaattcag
      541 catttttccc agacgggttt ccatctgata aatgtgtagc aaaataatac ttatcatgat
      601 ttgtaattac gtaacctaca aaccacccat tattatactt cccgtttact atacctgtac
      661 ctgttttccc atacagttca tacttttcat ttttcttaat caataatgaa gaagataatt
      721 gattctttgc ttttttacta aaatggttat tttgttccat catattttta aaaactatta
      781 cttgttcaag attagatatt ttcaaactat cttccatcca atagctttta taacttccca
      841 aattttcatt accataattt aattgcttga gttgagccgc agtatagttc tttggtattt
      901 gattactaat acgttcgaag taccaattaa ctgaattttg cattgctgta tttaaatctt
      961 gttccttatt ccaagactcg aaaagataat gtttatgatt ccatgacata cgtgaatttt
     1021 tatcacttat aatatgacgg tcaagtccaa acagtgctaa ataaattttg taagttgaat
     1081 caggagaata tctttttcta ctttcttttt cattataaat atagtatttg tctttcttca
     1141 tgctgtacat aacgaaagat ccactatttg aaccaaaatt tttactttca tctaaaattt
     1201 ggtaatcgct ttgtaagggc tttttataat tataatcagt taaggattgc cccattaaaa
     1261 actggctttg aattatcatg ataaaaaaag taaaaataca aataaaaatt agaattaatt
     1321 ttgactgctt tttcagatcg gcttctttta tattaattaa tctacttttg agtaatgatt
     1381 ttttaccatt aaatgaatgg cttaatatat ttttgttgga aaatggggtt tttaatacgg
     1441 agtccataat agattccgca taagttttaa attcattttt gttaatatta ttaaggacaa
     1501 atctatcggc ttctacttca ttgtcatgaa ttatctttct ttttacaata tgtactaatg
     1561 gattgtaact cataataata gagaagatat taaagataat taaatgtaaa gtatctctat
     1621 ttttagcatg agcatattca tgcagtatta tatattttag tcttttatca attacacttt
     1681 taaaatatga actaggaatc aaaataatat atttcccata ccaaaaagtt attggagatt
     1741 gaatagcctc tgcttttcga atcacaatat ttttttatat tgatggttga aaagtatcgt
     1801 atctatttta tttttttcat tttcgtttag ataaagtgac tgtttcttta aatatttaag
     1861 atataataag gctttcaaaa atttaaaact taaaataata actaaaacta tccaaataac
     1921 tgtgcagata ttatcaattg aatcccaatt aaacttatgg atatctgttg tgaactcttg
     1981 aataggtttg gtggtattta tgttatggtt caaatcgtgt gacttacttt caactgtggg
     2041 cgcttgatta ttcacattat taaattttat aaaagagaat ttaattggga taaaaggaat
     2101 taatccagca agcatagtta gataccaaac tttataattt aaactataat taaaatagcg
     2161 ttttaatatg tacctaaaaa atactaacaa tagaaatata aaacaaaagc ttactacgct
     2221 cattattagt aatttagcca ttttgacacc ttctttcaaa tatttataat aaacaattga
     2281 catcaatatt acaattgtaa tattattgat ttataaaaat tacaactgta atatcggagg
     2341 gtttattttg aaaaagttaa tatttttaat tgtaattgct ttagttttaa gtgcatgtaa
     2401 ttcaaacagt tcacatgcca aagagttaaa tgatttagaa aaaaaatata atgctcatat
     2461 tggtgtttat gctttaaata ctaaaagtgg taaggaagta aaatttaatt cagataagag
     2521 atttgcctat gcttcgactt caaaagcgat aaatagtgct attttgttag aacaagtacc
     2581 ttataataag ttaaataaaa aagtacatat taacaaagat gatatagttg cttattctcc
     2641 tattttagaa aaatatgtag gaaaagatat cactttaaaa gaacttattg aggcttcaat
     2701 gacatatagt gataatacag caaacaataa aattataaaa gaaatcggtg gaatcaaaaa
     2761 agttaaacaa cgtctaaaag aactaggaga taaagtaaca aatccagtta gatatgagat
     2821 agaattaaat tactattcac caaagagcaa aaaagatact tcaacgcctg ctgctttcgg
     2881 caagacttta aataaactta tcgcaaatgg aaaattaagc aaaaaaaata aaaatttctt
     2941 acttgattta atgtttaata ataaaaacgg agacacttta attaaagatg gtgttccaaa
     3001 agactataag gttgctgata aaagtggtca agcaataaca tatgcttcta gaaatgatgt
     3061 tgcttttgtt tatcctaagg gccaatctga acctattgtt ttagtcattt ttacgaataa
     3121 agacaataaa agtgataagc caaatgataa gttgataagt gaaaccgcca agagtgtaat
     3181 gaaggaattt taatattcta aatgcataat aaatactgat aacatcttat agtttgtatt
     3241 atattttgta ttatcgttga catgtataat tttgatatca aaaactgatt ttccctttat
     3301 tattttcgag atttattttc ttaattctct ttaacaaact agaaatattg tatatacaaa
     3361 aaatcataaa taatagatga atagtttaat tataggtgtt catcaatcga aaaagcaacg
     3421 tatcttattt aaagtgcgtt gcttttttct catttataag gttaaataat tctcatatat
     3481 caagcaaagt gacaatagca ttttacaagt attttttatt gttcgattag ggtaaaccta
     3541 aataatacat aaattatatt acaatatgtt tattatagag gctaaagtat cttagaaatt
     3601 tggatagtag gtttttcggg aataaaagta tagttggctt taatatataa ttaagctaag
     3661 ctatcattta tatttactcg attataattc gcacttcaac ttttttataa cgtaaaaatt
     3721 aaaacgtagt tttatttatc tttagtaata tctttttaaa cactacagtt tcttcattag
     3781 ttaaattagc gaagtaatga ttattaagtt gataggcttt cttttctaat gttggccata
     3841 agtcttttgc tttatttgta ctgtttatta gcacagttct tctattttca ttatcagttt
     3901 gacgtgttat tagatccttt ttggacatct tatctaatat tccagatatt gttgaaggtt
     3961 ctaaatttaa agtattacca atcgctttag gtgtattcaa attactatta atcaagcaat
     4021 tcaaaacacc atattctgct ggagtaatat tatagggact taataaactt ttaaaatgtt
     4081 taaaaactct attctgagaa gtactcaaca aataatttat acaattatct aattccacaa
     4141 ctaaaacctc tcttaaatca ttagtattga ctcgcttatc atatcatgat aagttttgta
     4201 ggaaaatagt tttgcaacaa aaacatactt ctacaaaaca attgtgaaag aaagctaata
     4261 aatgttcaat gcggtagtag tgagaaaaag tgcatcaaat gttagttata aagtagaaaa
     4321 agttgatgaa tctcatttat cgaagggtga tgtattagta aaagtggtat actcttcaat
     4381 aaattataag gatatgctag ctttacaata taaaggcggt gtaattagag attatcctat
     4441 gattcctggt atagattttg caggtattgt tgaatcaagt tcgaatgata agtttaaaga
     4501 aggcgataat gtattagtta ctggttatga tgttggcgtg acacatacag gtggattttc
     4561 agaatatgca caagtgcctg gagagtggat tgttcctatt ccccaagaaa tgtctattaa
     4621 agagtctatg gtatatggca cagccggttt tacggcagca ttatcaattt ttgaactaga
     4681 aaaatatgga atgaatatca aatcacaacc tgaaatttta gtgtctgggg ctacaggtgg
     4741 tgtgggcagt gttgctatac aaattttaca taaaattgga tatgaaaata tcactgctat
     4801 cgtaagaaaa gaaaagcaaa tagaaatcgc aaaaaaatta ggtgcaaaac aagtaataat
     4861 aattgaagat gataataaac cgcttaaaaa aggtattttt gattatttct tagatacagt
     4921 ggggagtaat gtaacattat acggtttaaa acgtctaaat tatcaaggtg ttgctactgt
     4981 atgtggaaat gtagcaggaa attcacttaa cgctaatata ttaccgttta ttcttagagg
     5041 tattaaatta attggcattg attctgtata tgttgatcat aatatccgtg aagatatatg
     5101 gagtaagtta gcaaatgaat ggaatatagg aaattcactt ctatacaatg aaattggatt
     5161 tgaagaattc tataaaacga tagatcagct tttaaaagga caacatttag gaagaactat
     5221 tttaaaagta taagtgtgag tattcattaa tacatttact attaattcaa tcattttgca
     5281 tttctctact aaccagtgtt ataatttata taacgaaaat gattaacaat taatgcacca
     5341 atccccacac tattgcagta gtgtggggat tttttcgatg ctggcttata aagtcgccta
     5401 ttaattattt gttgcgttta ctaagccaat aagtataata cgcaacagca cagccactga
     5461 tgactggtgc tatgatgtga acgaaaatga ttagcaaagt aatgcacctc ctctcttcgt
     5521 caattgacgc ctgagagata ggcgactcta ttattatacc atctgtgaat atacaagcat
     5581 atctacttcc gataagtcgt cttatgttat ataactttgt tttttatagt atcaagacaa
     5641 gaagaaactc gtttcaaaaa ccctcatgat ttttagagaa aaactctctt atttcatcgt
     5701 ctttaataaa ataatatgcc atctttccat ctttataaaa atcaagtacc tcatttttat
     5761 ataaaagcct taaatgatgt gaagttgaag caacactcat tttcaatatc aaagaaatat
     5821 cacaaacaca caactcatct tctttaatca aagataatat gattttcaat ttcttctcat
     5881 cacaaatttt ttctaaaatg ttaagtaatt tcttagattt atcatcttct aaaaaactta
     5941 aagcattgtt aactttatcc tcatgtacac agatcacatc acaagtattt tcataactca
     6001 tataaaatca caccctaaat taaaatataa atcctaaaat tgtttgaata gtgtcatttt
     6061 caataataat aaataaacct aaagctatat aaataatagc cataatccaa cgactaaatt
     6121 tctcaacaat ttctccaatt cctggaatat tagctaattt ttgtgcagta aacaccaaga
     6181 agaaaattaa aattaaaaag acaaacagag taagtaataa attagtaaca cttaatgtca
     6241 caaaatacgg aacaaataaa ccaatattat cggcgccaca acttgctatc gtaacaattg
     6301 caaccgtacc aactaattta gacaatcctt tttcattcaa ttctttttta gctctctttt
     6361 ctccttcaca atcatcataa atagccactt taattccaag ataaattggt attaaaccca
     6421 ataaacctaa tatccacttt tctggaacat aatttaatac aaaagctaaa aacaaactaa
     6481 ctaatattaa aataatagac cctaaatatt gaccaacata aatatctcta tattcttttc
     6541 tagtctttgc tctagcaaaa aatattaata aaattactaa taaatcaact gctgtagcaa
     6601 tataaagaac agcagcagca acaaccgttt gaatcataat acacctcatt caaaaatatt
     6661 tttgaatgta ttataactta tatttttaaa taatcatagg tgctttattc caattg
//
LOCUS       DAOBBH010000134         6647 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.134, whole genome shotgun sequence.
ACCESSION   DAOBBH010000134 DAOBBH010000000
VERSION     DAOBBH010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.134"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..515
                     /locus_tag="P6L01_001971"
     CDS             <1..515
                     /locus_tag="P6L01_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3031023.1"
                     /translation="INEKQNEWRQRLMVTQFTFTSYYISKVVKTMLQFAIAILVIFMV
                     GHFYKGVAMSAVQWLESGIFLWLGASLLITFGILFSLLNDIQKTSALANIVTIGLAVL
                     GGLWFPINTFPNWLQHVAHVLPSYHLRKLGVDIASNHHINLISFAIILLYVLGSIIAV
                     YCISHFKRAE"
     gene            519..1610
                     /locus_tag="P6L01_001972"
     CDS             519..1610
                     /locus_tag="P6L01_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDP3031024.1"
                     /translation="MKFLKDTSIAEISSILYLIFPIAGIFFNEVYGPKWLYIISVIVF
                     SLSYLILVIVNNRLNTLMFYILLIIHYFIICYFVFSVHPMLSLFFFYSAFAVPFTFKN
                     NVKKTATNLFILTMIICTIITYLLYNNYFVAMMVYYVVISLIMLDNFKKMKNREYQKE
                     IAEKNRHINTLIAEQERHRIGQDLHDTLGHVFASLSLKSELAYKLIDTDVEKVKAELL
                     AINKLSRESLNKVREIIDDVKLPSFIEEIDSIRKVLKDADIDFTFENKELAQVLSPTK
                     QSMLVMITREAINNVIKHANASKVHGKLKTVNNHKLLLMIEDDGKGIDSDCEVKSISQ
                     RVQHLNGTLAVDSTNGTKIIIEISTGGIA"
     gene            1607..2209
                     /locus_tag="P6L01_001973"
     CDS             1607..2209
                     /locus_tag="P6L01_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDP3031025.1"
                     /translation="MTSLIIAEDQNMLRQAMVQLIKLHGDFEILADTDNGLDAMKLIE
                     EYNPNVVILDIEMPGMTGLEVLAEIRKKHLNIKVIIVTTFKRPGYFEKAVVNDVDAYV
                     LKERSIEELVETINKVNNGEKEYSATLMTSFFVDKNPLTPKEQIVLREIGNGLSSKEI
                     SEKLFLTDGTVRNYTSVIIDKLFADNRFDAWKKANEKGWI"
     gene            complement(2322..2510)
                     /locus_tag="P6L01_001974"
     CDS             complement(2322..2510)
                     /locus_tag="P6L01_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HDP3031026.1"
                     /translation="MNISNENTVTLIAVIIAIIIGIFLQIFFKLPLIVTAVLSILLGI
                     FVGFIVYLIVSFYNKRKN"
     gene            2649..3182
                     /locus_tag="P6L01_001975"
     CDS             2649..3182
                     /locus_tag="P6L01_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDP3031027.1"
                     /translation="MKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLN
                     GKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKT
                     LTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVF
                     IEAQNKAKQQKLNIWSK"
     gene            complement(3327..4181)
                     /locus_tag="P6L01_001976"
     CDS             complement(3327..4181)
                     /locus_tag="P6L01_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031028.1"
                     /translation="MSFYVVLIIIIVALIGILVLNQRYSNSKIDTEVYARKQLIKKNK
                     ALSAENAELRSQMLSSNNDVGHHAYKNAKRELRKILDSYLENGKLKYYDIIVTSNLAT
                     KHPFFEYARSFDFIIVSDIGLINVDVKSWGEKTFYHFDVPDEHDTEMSNSNIEKVVGH
                     YISQQYHDQFNSSRKSIYTFTETVQPNRVIYDFYDYDPYQLAANNAKALKDHIEQNFN
                     FKVQSTGVIYFSDGTVNIIQGSEERDKYVDTVSTKSSLRRIISEAIELSKHPLNKEQV
                     DQITAIFK"
     gene            4512..5087
                     /locus_tag="P6L01_001977"
     CDS             4512..5087
                     /locus_tag="P6L01_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031029.1"
                     /translation="MNAKEQLVDNLMKTSSQLFKFHGEVAMQLFLNDELKLPSIVEIC
                     VERKRLSDIVKVIPQSYALLYIDKQDQAIAKEDLSLSKIAKVYVQYDDTTIMSIFVYD
                     VVNDEWIFRLDPNIRIPKSNIYFHSLNWDVDYIKPEIVLMYDLMQHHQYHHYSNYKRV
                     IDALSYYQFFILKFVVGEQRIKDAIQRTINN"
     gene            complement(5141..6523)
                     /locus_tag="P6L01_001978"
     CDS             complement(5141..6523)
                     /locus_tag="P6L01_001978"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDP3031030.1"
                     /translation="MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDN
                     DIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYIN
                     QLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
                     DSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENF
                     TDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKN
                     TNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLED
                     NNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGE
                     GMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHG
                     VALKNLCDKN"
BASE COUNT         2368 a          892 c         1035 g         2352 t
ORIGIN      
        1 ttattaatga aaaacaaaat gaatggcgcc aaagattaat ggtaacacaa tttactttta
       61 ctagttatta tatttcaaaa gtagtgaaaa ctatgctgca atttgcaata gcgatattag
      121 ttatttttat ggttggacat ttttataaag gtgttgcaat gagtgcagtt caatggttag
      181 agtcaggaat atttttatgg ttaggtgcgt ctctattaat aacttttggc atattatttt
      241 ctttgttaaa tgatattcaa aaaacaagtg ctttagctaa tatcgtaaca attggtttag
      301 cagtattagg tggattgtgg tttccgataa acacatttcc aaattggctt caacatgttg
      361 ctcatgtttt accgagctat catttgcgta aactaggtgt agatattgct tcaaatcatc
      421 atatcaattt aatatcattt gctataatac tcttgtatgt tttagggagt ataatagcag
      481 tatattgtat tagtcatttt aaaagggcgg aataaaatat gaaattttta aaagatactt
      541 caattgctga aatatcgtct atactttatc tgatttttcc tattgccggt atatttttta
      601 atgaagtata tggtcccaaa tggttgtata ttatatcagt cattgtcttt tcgttgtcgt
      661 atcttatatt agttatagta aataatagac ttaatacatt aatgttttac attttgttga
      721 ttattcatta ttttattatt tgttattttg ttttcagtgt acatccaatg ctaagtttgt
      781 ttttctttta tagtgctttt gccgttccat ttacttttaa aaataatgtt aaaaaaacgg
      841 caactaatct tttcatacta acaatgatta tatgtacaat aataacgtac ttattgtata
      901 acaactattt tgttgcaatg atggtttatt atgtcgttat atcgttaata atgctagata
      961 attttaaaaa aatgaaaaac cgtgaatatc aaaaagaaat agcagaaaaa aatagacata
     1021 ttaatacatt aattgctgaa caagagcgac atagaattgg tcaagactta catgatacgt
     1081 tagggcatgt gtttgcaagt ttatcattaa aatcagaatt agcttataaa ctaatagata
     1141 ctgatgtaga aaaagtaaaa gctgaattat tagcaattaa taaattatct cgtgaatcat
     1201 tgaacaaagt tcgagaaatt attgatgatg taaaattacc atcatttatt gaagagattg
     1261 atagtatacg taaagtttta aaagatgctg atattgattt tacatttgaa aataaagaat
     1321 tagcgcaagt attaagtcct actaaacaat ctatgttagt tatgattacg cgtgaagcga
     1381 taaataatgt tattaaacat gcaaatgctt caaaagttca tggtaaatta aaaactgtaa
     1441 acaatcataa attactgctt atgattgaag atgatggcaa aggtatcgat agtgattgtg
     1501 aggtgaaaag tatttcacag cgtgtacaac atttaaatgg aactttagca gtcgactcaa
     1561 caaatggaac taaaataatc attgaaatct caacaggagg aatagcatga catctttaat
     1621 tattgcagaa gatcaaaata tgttacgaca ggcaatggtt caattaatta aactacatgg
     1681 tgattttgaa attttagcag atactgataa tggtctcgat gcaatgaaac ttattgaaga
     1741 atataatcct aacgttgtta ttttagatat agaaatgcca ggcatgactg gacttgaagt
     1801 tttagcggaa attagaaaaa agcatttgaa tattaaagtg attattgtaa caacttttaa
     1861 aagaccggga tactttgaaa aagcagttgt gaatgatgtg gatgcatatg ttttaaaaga
     1921 acgttctata gaagaattgg tggaaaccat taataaagta aataacggag agaaagaata
     1981 tagcgccaca ttgatgactt cattttttgt agataaaaac ccattaacgc ccaaagaaca
     2041 aattgtatta agggaaattg gcaatggttt aagtagtaaa gaaataagtg aaaaattatt
     2101 tttgacagat ggaacagtta gaaattatac atctgttata attgataaat tatttgcaga
     2161 taatcgtttt gatgcttgga aaaaggcaaa tgaaaaaggc tggatctaaa tacaaacaaa
     2221 aaagtattga gtgttttgat ttaagagact tggaaagtga gtcaataatc aaaatcttac
     2281 tcaatacttt tattttggta ctcgcaattt agatttgtta actaattttt tcgcttatta
     2341 taaaacgaaa caatcaagta aacgataaag cctacaaaga tacccaataa aatagatagt
     2401 actgccgtca ctattaatgg taatttgaaa aatatttgta ggaaaatacc aatgataatt
     2461 gcgataatta ctgcaattaa tgtgactgta ttttcatttg aaatgttcat ttattttcac
     2521 tccttaacaa taacattata tcatgctata gctttccaaa atattgaaat atgtagatat
     2581 ggctattgac gatatttctt aacttttata tgattaatcg gaatgaaaaa agagaagtag
     2641 gtggcaatat gaagtcaaat aaatcgcttg ctatgattgt ggtagccatc attattgtag
     2701 gtgtattagc atttcaattt atgaatcata cgggtccttt caaaaagggg acgaatcatg
     2761 aaactgtaca agatttaaat ggtaaagata aagtacatgt tcaaagagtt gtggatggtg
     2821 atacatttat tgcaaatcaa aatggtaaag aaattaaagt taggcttata ggggttgata
     2881 cgccagaaac ggtgaaaccg aatacgcctg tacaaccatt tggcaaagaa gcatcaaatt
     2941 atagtaagaa gacattaaca aatcaagatg tttatttaga atatgataaa gaaaaacaag
     3001 atcgctatgg tagaacattg gcgtatgtat ggataagtaa agatcgtatg tacaataagg
     3061 aattagtgga aaagggactt gctagagaga agtatttttc accaaatggc aaatatagaa
     3121 atgtatttat agaagcacaa aataaagcta aacaacagaa attaaatatt tggagtaaat
     3181 aattaaatag gtgattatat atattaaagt aaaatatact tgaagcatcg atttattatt
     3241 accataactt tatatataaa gaaataattt aaaggcataa taaccatagt gcgtatcgag
     3301 cgtaatggat tattatgcct ttgattttac ttgaatatag ctgtgatttg atcaacctgt
     3361 tctttattta aagggtgttt agatagctct atggcttcgc taataatacg tcgtaatgaa
     3421 gattttgttg aaacggtatc aacatattta tctctttctt cagaaccttg aatgatatta
     3481 actgtaccat cactaaagta aataacaccg gtactttgga ctttaaagtt aaagttttgt
     3541 tcaatatgat cctttaatgc tttagcatta tttgctgcta attgatatgg atcataatca
     3601 taaaaatcat aaataacacg atttggttga actgtttctg taaaagtata gatagacttt
     3661 cttgatgaat taaactgatc atgatattgc tggctaatgt aatgaccgac aactttttca
     3721 atattactat tgctcatttc tgtatcatgc tcatcaggta catcaaagtg ataaaatgtt
     3781 ttttcacccc aactttttac atcaacgttt attaaaccga tatctgaaac aatgataaaa
     3841 tcaaatgaac gtgcatactc gaaaaacgga tgtttagtag ctaaattgct tgtaacgata
     3901 atatcgtaat attttaattt accattttcg agatagctat ctaaaatctt gcgtaattct
     3961 cttttagcat ttttgtatgc atggtgtcca acatcattat tagaactcag catttgactt
     4021 cttaattcag cattttctgc acttaatgct ttattctttt taataagttg ctttcttgca
     4081 taaacttcgg tatctatttt actattacta tacctttgat ttaaaactaa tataccaatt
     4141 aatgctacaa tgataatgat aagtacaaca taaaaagaca ttttttcacc aatccttttt
     4201 gacttcttta actttgtata caataataat taataaagat taattgttat tcaatttccc
     4261 acatttttat tagttgattt tagttcatca ttgttataat caaattataa actgacagat
     4321 attgatgttc aatgaatatg acgtgaaaga ttcgtgaatt caagtttatg tcgaatttat
     4381 gttataacgg tcatttaaat gacagaatta ggtcactcat agtattttga agattgaatt
     4441 cattaatttt aaaatgtata atgatatttg tgaaagcgct tgcttaggag gtgtatttga
     4501 gagtgaatga aatgaatgct aaagaacaat tagtggacaa tttaatgaaa acatcatcgc
     4561 aattatttaa atttcacggt gaagttgcca tgcagctttt cttaaatgat gaattaaaat
     4621 taccttctat tgttgaaata tgcgtggaac gtaagcgttt aagtgatatt gtgaaagtta
     4681 ttccgcaatc atatgcgtta ctatacatag ataagcaaga tcaagcaata gctaaagaag
     4741 atttatcact ttcaaaaatt gcaaaagttt atgtgcaata tgatgataca acaataatga
     4801 gtattttcgt ttatgatgta gtaaacgatg aatggatttt tagattggat ccgaatatac
     4861 gtatacctaa gagtaacata tacttccata gtttaaattg ggatgtggat tatattaaac
     4921 cggagatcgt tctaatgtat gatctaatgc aacaccatca gtatcatcat tattccaatt
     4981 ataaacgagt catagatgca ttaagctact atcaattttt tattttaaaa tttgtagtag
     5041 gtgagcaacg tattaaggat gcaatccaga gaacaataaa taattaagaa aaagcaattc
     5101 ataacgcagt tgaatacatg tgttacgaat tgcttttata ttagttttta tcacacaagt
     5161 tttttaatgc aaccccgtga tagcaaaact catatgtaga taatacagct ttttcagcat
     5221 catctacatg aattccaaac atcatcgaga tttccgaagc accttggtta atcattttta
     5281 agttaatttt tgattcagct aaggcatgtg taattttatt tgcagtaccg atgactttat
     5341 tcataccttc gccgacaatc attaatattg ctaaatcatg ctcaatactt agctcatcaa
     5401 catcacattt ttgacgaatt tcatttaata cttttgtttc tttattttga atttgttttg
     5461 aacgcataac gatactgata gtatcaatac ctgaaggcat atgatcaaat gaaatattat
     5521 tatcctctaa gacacctaat atctttctag taaagccgac ttgtctattc attaaatact
     5581 ttttgatatt aatgacagta aaatctttat cacaacttat accgctaatc acatttttcg
     5641 catttatttc tctatcatgc actataaatg tacccttatc ttgagggcgg ttcgtatttt
     5701 taatcaccac agggatgcga tctttataaa gtggttgtaa ggcttcatca tggaaaacac
     5761 taaaaccagc ataagataat tcacgcattt ctcgataagt gatttcttcg attaattcag
     5821 gatctttgat gatatttgga ttagctttat aaataccaga cacatcggtg aaattttcat
     5881 aaattgtggc tctaacacca cttgatatga tggcgccagt tatatctgat ccgccacgtg
     5941 gaaatgtaac tatatatcct tcatgagata cgccaaaaaa tcctgggata attagttttt
     6001 catcataatc tcttaatttt ttaatttcag agtaagcact atctaatatt tgtgcttctt
     6061 gtgggacgtc agtaacaaaa atacccgctt ccttcggtga tatatatttt gttggtatac
     6121 cttgactatt attatataaa gctatcaatt gcgcattaaa atcttcacca caagaaagta
     6181 atgcatctaa tagtctcttt ggttcatttt ttaattgatt aatataatgt tccaaagtca
     6241 catctatcgt ccgtaaaata ctttcatcca tttgcaattc ttttacaata tcatcataac
     6301 gctgaataat ttctcttttt ttatcatgat aatcaagatg attaatgacc ttttcatata
     6361 atctgattaa caaatcagtt gttttaatat cattatcatg tcttttacct ggagcagaaa
     6421 cgataacaat ctttcgctct ggatcagaat taacaatatt taaaaccttt ttaatttgag
     6481 tagcattgga gacggagcta ccaccgaatt tggaaacttt catagtgcat gccaaccttt
     6541 ctaaaaaatc agaaaattgt attcagtttt gtaagtaaga atgatattat gtatttatat
     6601 tttgaatatt acgaacttta caaaaataaa gaataattct atactat
//
LOCUS       DAOBBH010000135         6640 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.135, whole genome shotgun sequence.
ACCESSION   DAOBBH010000135 DAOBBH010000000
VERSION     DAOBBH010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6640
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.135"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..2968)
                     /gene="clfA"
                     /locus_tag="P6L01_001979"
     CDS             complement(<1..2968)
                     /gene="clfA"
                     /locus_tag="P6L01_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfA"
                     /protein_id="HDP3031031.1"
                     /translation="MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSV
                     TQSDSASNESKSNDSSSVSAAPKTDDTNVSDTKTSSNTNNGETSVAQNPAQQETTQSS
                     STNATTEETPVTGEATTTTTNQANTPATTQSSNTNAEELVNQTSNETTSNDTNTVSSV
                     NSPQNSTNAENVSTTQDTSTEATPSNNESAPQNTDASNKDVVSQVVNPSTPRMRAFSL
                     AAVAADAPAAGTDITNQLTDVKVTIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFK
                     ITVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVDNKENVTANIT
                     MPAYIDPENVTKTGNVTLTTGIGTNTASKTVLIDYEKYGQFHNLSIKGTIDQIDKTNN
                     TYRQTIYVNPSGDNVVLPALTGNLIPNTKSNALIDAKNTDIKVYRVDNANDLSESYYV
                     NPSDFEDVTNQVRISFPNANQYKVEFPTDDDQITTPYIVVVNGHIDPASTGDLALRST
                     FYGYDSNFIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPG
                     SDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSA
                     SDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSE
                     SDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSDNDSDSD
                     SNSDSESGSNNNVVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLIWGLLAS
                     LGSLLLFRRKKENKDKK"
     gene            complement(3231..3761)
                     /locus_tag="P6L01_001980"
     CDS             complement(3231..3761)
                     /locus_tag="P6L01_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDP3031032.1"
                     /translation="MQIRQIHQHDFAQVDQLIRTAFENSEHGYGNESELVDQIRLSDT
                     YDNNLEIVAVLQNEVVGHGLLSEVYLDNGAQREIGLVLAPVSVDIHHQNKGIGKRLIQ
                     ALEREAILKGYNFISVLGWPTYYANLGYQRASMYDIYPPYDGIPDEAFLIKELKVNSL
                     AGKTGTINYTSAFEKI"
     gene            complement(3900..4421)
                     /locus_tag="P6L01_001981"
     CDS             complement(3900..4421)
                     /locus_tag="P6L01_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031033.1"
                     /translation="MINIISAIGSIGTFIMALFYFVSVSVQLYQMKISFLPALGFNQI
                     LLEREKDQLNIMNSATEEHHHKDYIKLYNLGGGAAKKIAIEVLLGNDKVIQKKYVNIL
                     PSKEGYMLPINKNVYEELERTIENNGYEADLNVRMTYYHNVSRKQQEVILKGQIDRFN
                     TYNNKEIYDLQFI"
     gene            complement(4429..4584)
                     /locus_tag="P6L01_001982"
     CDS             complement(4429..4584)
                     /locus_tag="P6L01_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031034.1"
                     /translation="MMVKKLLKAIKQNFLYEQSNITFKMIVFGYKRLSKMLFQFEINH
                     INFLWEG"
     gene            5019..5747
                     /locus_tag="P6L01_001983"
     CDS             5019..5747
                     /locus_tag="P6L01_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HDP3031035.1"
                     /translation="MKKVMGILLASTLILGACGHHQDSAKKESASHKKKENDNEELNE
                     ELKEFKSKKNMDIKIKGDTIVSDKFEAKIKEPFIINEKDEKKKYIAFKMEITAKKDDK
                     DLNPSSISHDYINITQDDKNTVNKLRDGYLLSDKKYKDWTEHNQDQIKKGKTAQAMFI
                     YELRGDGNINLNVHKYSEDKTVDSKSFKFSKLKTEDFSHRAETREEVEKKEKEFEEEY
                     KKEQEREKEKEKQKDDDHSSLDEV"
     gene            5838..6085
                     /locus_tag="P6L01_001984"
                     /pseudo
     CDS             5838..6085
                     /locus_tag="P6L01_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015728980.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6101..6424
                     /locus_tag="P6L01_001985"
     CDS             6101..6424
                     /locus_tag="P6L01_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031036.1"
                     /translation="MNNEELEMRLLLMKQSIEQLQEELAPNLKTRDLVLLRYMYSYKE
                     INMLDSYLFQLATNKEQITKKQFKTKLENIREVPEIPIRQVNDILEGYKNSELYVELI
                     NSILK"
BASE COUNT         2185 a         1126 c         1054 g         2275 t
ORIGIN      
        1 atttcttatc tttattttct ttttttcttc tgaaaagtag taatgaacct aatgatgcta
       61 ataatcccca aattagtgac gtattcgctt catcttcaga acctgtatct ggtaatggtt
      121 ctttactatc tttagcctca tttttattag aagcattagt accattttta ggtgaattag
      181 gcggaactac attattgtta gaacctgact cggaatcgct atttgaatca ctgtctgagt
      241 cgttgtctga tcctgtgtca ctcgttgaat cggaatctga atcacttgat gaatcggagt
      301 cactacctga gtctgaatca ctcgctgaat ccgaatcgct atctgaatcg gagtcactat
      361 ctgattctga atcgctgtct gaatcggagt cactatctga ttctgaatcg ctgtctgagt
      421 ctgagtcact atctgaatct gaatcactgt ctgagtctga gtcactatct gattctgaat
      481 cgctatctga atctgaatca ctcgctgaat ccgagtcact atcggaatct gagtcactgt
      541 cggaatctga atcgctatcg gaatctgaat cgctgtctga gtctgagtca ctgtcggaat
      601 ctgaatcgct atctgagtct gaatcactgt ctgaatctga atcgctatcg gaatctgagt
      661 cactgtctga gtcagaatcg ctgtctgaat ctgaatcgct gtctgaatct gagtcgctat
      721 ctgagtcgga atcgctatct gagtcggaat cactgtcgga atctgaatcg ctatcggaat
      781 ctgagtcact gtctgagtcg gaatcgctat cggaatctga gtcactgtct gagtcggaat
      841 cgctatcgga atctgagtca ctgtctgagt cagaatcgct gtctgagtca gaatcgctgt
      901 ctgaatctga gtcgctatct gagtcggaat cgctatcgga atctgagtca ctatctgagt
      961 ctgaatcgct cgctgagtcg gaatcactcg ctgaatctga atcgctcgct gaatctgaat
     1021 cgctcgctga atctgaatcg cttgctgaat ctgaatcact tgctgaatct gaatcacttg
     1081 ctgagtctga atcacttgct gaatctgaat cacttgctga atctgaatca cttgctgaat
     1141 ctgaatcact cgctgagtct gaatcacttg ctgaatctga atcactcgct gaatctgaac
     1201 cactatctga tgtagaatca ctgccagaat ctgaaccgct atctgaatta gaatcgctgc
     1261 cagaatctga acctgggtca gaatctgaat cctctggaat tggttcaatt tcaccaggct
     1321 catcaggttg ttcaggaaca actggtttat cgataccgtc accagaacct gatccgttat
     1381 taaatgctac ttcgttgtcc catgacatag atctccatat aaaattagaa tcataaccat
     1441 aaaatgtcga acgtagtgct aaatcacctg tactagcagg atcaatatgg ccattaacaa
     1501 ctacaatata cggtgttgta atttggtcat cgtccgtagg aaattctact ttgtattgat
     1561 tagcatttgg aaatgaaatt ctaacttgat tagttacatc ttcaaaatcg ctaggattca
     1621 cataataact ttcagataaa tcattagcat tatcgactct ataaacttta atatcagtgt
     1681 tttttgcatc tattaacgca ttactctttg tattaggaat taaattacct gttaaggcag
     1741 gtaacacaac gttatctccg cttggattga cataaattgt ttggcgatac gtattatttg
     1801 ttttatcgat ttgatcaatc gtacctttaa ttgataaatt atggaattgt ccatatttct
     1861 catagtcgat taatactgtc ttactagcag tattggttcc tatgccagtt gtcaatgtca
     1921 cattacctgt ctttgtaaca ttttcagggt caatataagc tggcatagta atattagctg
     1981 ttacattttc tttattatca acatagtctg taaatgtata aataacatta ccatcactat
     2041 cgattacacc atttgccaat acttgatctc cagccataat tggtggcact ttagcagttg
     2101 aagttacacc atttaagttt aattctttag gtacagttat tttgaatgtg tcacctttaa
     2161 cagcagaatt aggcactgaa aaaccataat tcagtttgac ataacctgct tggtgcggat
     2221 acacagtcgt accagagtca atagtaactt tcacatctgt caactgattc gtaatatctg
     2281 tgccagctgc cggtgcatct gcagctactg ccgctaaact aaatgctctc attctaggcg
     2341 tacttggatt aaccacttga ctaactacat ctttattact tgcatctgta ttctgtggag
     2401 ctgattcatt gtttgaaggt gttgcttcag ttgaagtatc ttgcgttgtt gaaacatttt
     2461 ccgcatttgt agaattttga ggtgaattta cagatgatac tgtattagta tcattagaag
     2521 tcgtttcatt acttgtttga ttcactaatt cctccgcatt tgtattgctt gattgagttg
     2581 ttgccggtgt attagcttga ttcgttgtcg tagtagtagc ttcaccagtt accggcgttt
     2641 cttccgtagt tgcatttgtt gatgatgatt gtgtcgtttc ctgttgtgct ggattttgcg
     2701 ccacactcgt ttcgccatta ttagtgtttg acgatgtttt agtatcactc acgtttgtgt
     2761 cgtctgtttt aggtgcagca ctaacgctac ttgaatcatt acttttgctt tcgttacttg
     2821 cgctatcaga ttgcgtaaca ctattttcac ttgcatctgc ttctttactg ctgagtagtc
     2881 caaaaccgat taacgtacct acaagcactg aagccacgcc aatcgatttt ttccgaattg
     2941 cgtgtttttc ttttttcttc atattcattt tattccctct ttttaaaaag tcattttata
     3001 ttaactatat accctttaaa gatatattta atctctgtta atggaattat acactaaaat
     3061 tgcattatag caattaattt gtatcgatat tttattatcc acaataatac tttactaaca
     3121 aacattttat ttattgctat tttaagaatt acaaacgaca acgtacgatt tgattgcaaa
     3181 cattttttat tattaatatg aactctacct aatgtaatcc tagctttaaa tcatattttt
     3241 tcaaaagcag atgtgtaatt tatggtacct gtttttcccg ctaaactgtt cacttttaat
     3301 tctttaatta aaaacgcttc gtctggtata ccatcatatg gtggataaat gtcgtacata
     3361 cttgcgcgtt gatatcctag attggcataa tacgtcggcc atcctaatac actgataaaa
     3421 ttatatcctt ttaatattgc ttctcgttct aatgcttgaa tcaatcgctt cccaatacct
     3481 ttattttgat gatgaatatc aacagataca ggtgctaaca ctaatccaat ttcccgttgt
     3541 gctccgttat caagataaac ttcacttagt aaaccgtgcc ctacaacttc gttttgaaga
     3601 acagctacta tttctaagtt attgtcatac gtatcactta gacgaatttg gtctactagc
     3661 tctgattcat taccataacc atgttcacta ttttcaaatg ccgttctaat taactggtca
     3721 acttgagcaa agtcatgttg atgtatttgt ctaatttgca tctttgcccc tccttttagt
     3781 tctatcttat agaaaaatgc attacaacat aaagtaatat tttatagtaa tgacgccatt
     3841 atttcattaa aattgaatga tatttttcgc aataaacaac taccctctta aatcaatttt
     3901 tagataaact gcaaatcata aatttcttta ttattataag tattaaaacg gtcgatttga
     3961 ccttttaata taacttcctg ttgtttgcga cttacattat gataataagt catacgtaca
     4021 ttcaaatcag cttcataacc attattctca atcgttcttt ctaattcttc gtacacattt
     4081 ttattaattg gtaacatgta cccttcttta ctaggtaaaa tattcacgta ttttttctga
     4141 atgactttat cattacccaa taaaacctct attgcaattt ttttagcagc accgccacct
     4201 aaattatata gtttaatata atctttatga tgatgctctt ctgttgccga attcattata
     4261 ttaagttgat ccttctccct ttctaataaa atttggttaa aacctaaagc tggcagaaag
     4321 ctaattttca tttgataaag ttgaactgaa actgatacaa aataaaataa agccataata
     4381 aatgttccaa tagatcctat agctgaaatg atattaatca ttaagatatc aaccttccca
     4441 taagaaattt atatgattaa tttcaaactg aaaaagcatt ttacttaatc gtttatatcc
     4501 aaaaacaatc attttaaacg ttatattcga ctgctcatat aaaaagtttt gctttatagc
     4561 cttaagtaat tttttaacca tcaagtacga ttattattac ggcaatctat ttgcattgac
     4621 taaaaaatac aataacgctt ttaagagctc ccatctttga ccagtatttt ccaaattaat
     4681 aacacatctt cttcaataca tattcatctc atacaaataa gttgattttt ttatttttac
     4741 aaacgtaatt tcttaacaaa tcaaaatttt acatttaaat ctttcgatat attacaagtt
     4801 ttttaaaaaa gtaatatatt tctatcaata aaagtaatat aatattagta attatagttt
     4861 cattaattta ttgcaacaat acataggatc attattacaa gaattattta atatcatctt
     4921 agaatctcac aaaatatcaa ctttgtttaa ttactcaaaa tgataagtca tttagagttt
     4981 ttaaaataaa tttttgcgaa ataaaggaga catgttaaat gaaaaaagta atggggatat
     5041 tattagcaag tacacttatc ttaggtgctt gtggacatca tcaagatagt gcaaaaaaag
     5101 agagcgctag tcacaaaaag aaagaaaatg acaatgaaga attaaatgaa gaacttaaag
     5161 aatttaaaag caaaaaaaat atggatataa aaattaaagg cgatactatt gttagtgaca
     5221 aatttgaagc taaaataaaa gaaccgttta tcatcaatga aaaagatgag aaaaagaaat
     5281 atatcgcttt taaaatggaa attactgcta aaaaagacga taaagattta aatccatctt
     5341 ctatttctca tgactatatt aatatcactc aagatgataa aaatacagta aataaattaa
     5401 gagatggtta tcttttaagt gataaaaaat ataaagattg gacagaacat aaccaagatc
     5461 aaattaaaaa aggcaaaact gcacaagcca tgtttatcta tgagttaaga ggtgatggaa
     5521 atattaattt aaatgtccat aaatactcag aagataaaac agttgattct aaatcattca
     5581 aatttagtaa acttaaaacc gaagattttt ctcatagagc ggaaacaaga gaagaagtag
     5641 aaaagaaaga aaaagaattt gaagaagagt acaaaaaaga acaagaacga gagaaagaaa
     5701 aagaaaagca aaaagatgac gaccacagta gtttagatga agtataaatt tggtttgttg
     5761 attgtcttac attcagttat ttaattttta gagaaactga cttatttata gcttagccaa
     5821 catgatacta taaaactatg attaaaaata aaaaatctgt gtttaatggt gttaaagaaa
     5881 tcagtccccg tgccggaaaa tcataaaaaa aagatgggat aaatttgtaa atcccgcatt
     5941 gaataatgca ttaaaacaag aagaggctac atggggaaat gtacaaggtc aagtttcaca
     6001 agcattgatg ggaactggta ttaaagattc tactgcaaga agcatagggt tttgggtttc
     6061 ccaagttggt caagcattaa tataaaaaga gtgtgataaa atgaataatg aagaattaga
     6121 aatgagattg ttattaatga aacaatcaat agaacaatta caagaagaac tagcgcctaa
     6181 cttaaaaact agagatttag tgttattaag atatatgtat tcatataaag aaattaatat
     6241 gctagattct tacttgttcc aattggctac aaataaagag caaataacaa aaaaacaatt
     6301 taaaacaaaa ttggaaaaca ttagagaagt accagaaata cctattagac aagttaatga
     6361 tatattagaa ggttataaaa atagtgagtt atatgttgag ttgataaata gcatcctaaa
     6421 ataaaaataa acatactaag attagctatg aaggaatcta tgacgataga ttttttcata
     6481 gctatttttt atagttataa agaggagtag tctgttctga ctcttggatt ttaaatctgt
     6541 ataataaaca agtaagcttt actctcgcct tttaaaattc gtttgtagta tgttgggttc
     6601 ttaaaatcgt gaataggaaa atgcaatgag aaaggttaag
//
LOCUS       DAOBBH010000136         6637 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.136, whole genome shotgun sequence.
ACCESSION   DAOBBH010000136 DAOBBH010000000
VERSION     DAOBBH010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.136"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(256..756)
                     /locus_tag="P6L01_001986"
     CDS             complement(256..756)
                     /locus_tag="P6L01_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDP3031037.1"
                     /translation="MITYKNILIAVDGSHEAEWAFNRAVGVAKRNDAKLTIVNVIDSR
                     TYSSYEVYDAQFTEKSKHFAEELLNGYKEVATNAGVKDVETRLEFGSPKSIIPKKLAH
                     EINADLIMSGTSGLNAVERFIVGSVSESIVRHAPCDVLVVRTEELPADFQPQVATTQL
                     REKYQN"
     gene            897..2015
                     /gene="ald"
                     /locus_tag="P6L01_001987"
     CDS             897..2015
                     /gene="ald"
                     /locus_tag="P6L01_001987"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HDP3031038.1"
                     /translation="MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSF
                     FEDVDYKEAGAEIVAEQAKVWDVDMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKL
                     TQALIDRKVISIAYETVQLPDRSLPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLG
                     GVPGVPKGKVTIIGGGQAGTNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIM
                     SNPLNIELYVKQSDLVIGAVLIPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFET
                     TDKITTHDDPTYIKHGVVHYAVANMPGAVPRTSTLALNNATLPYALMLANKGYREAFK
                     SNQPLSLGLNTYKGHVTNKGVAEAFEMEYKSVEEALQL"
     gene            2139..2237
                     /locus_tag="P6L01_001988"
     CDS             2139..2237
                     /locus_tag="P6L01_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031039.1"
                     /translation="MNDIDLIHYMLLMNIPAYVFLACLLHIAFRLG"
     gene            complement(2331..3386)
                     /locus_tag="P6L01_001989"
     CDS             complement(2331..3386)
                     /locus_tag="P6L01_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDP3031040.1"
                     /translation="MTKISKIIDELNNQQADAAWITTPLNVYYFTGYRSEPHERLFAL
                     LIKKDGKQVLFCPKMEVEEVKASPFTGEIVGYLDTENPFSLYPQTINKLLIESEHLTV
                     ARQKQLISGFNVNSFGDVDLTIKQLRNIKSEDEISKIRKAAELADKCIEIGVSYLKEG
                     VTEREVVNHIEQTIKQYGVNEMSFDTMVLFGDHAASPHGTPGDRRLKSNEYVLFDLGV
                     IYEHYCSDMTRTIKFGEPSKEAQEIYNIVLEAETSAIQAIKPGIPLKDIDHIARNIIS
                     EKGYGEYFPHRLGHGLGLQEHEYQDVSSTNSNLLEAGMVITIEPGIYVPGVAGVRIED
                     DILVTNEGYEVLTHYEK"
     gene            3561..4250
                     /locus_tag="P6L01_001990"
     CDS             3561..4250
                     /locus_tag="P6L01_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDP3031041.1"
                     /translation="MKLSFHGQSTIYLEGNNKKVIVDPFISNNPKCDLNIETVQVDYI
                     VLTHGHFDHFGDVVELAKKTGATVIGSAEMADYLSSYHGVENVHGMNIGGKANFDFGS
                     VKFVQAFHSSSFTHENGIPVYLGMPMGIVFEVEGKTIYHTGDTGLFSDMSLIAKRHPV
                     DVCFVPIGDNFTMGIDDASYAINEFIKPKISVPIHYDTFPLIEQDPQQFKDAVNVGDV
                     QILKPGESVQF"
     gene            complement(4555..4968)
                     /locus_tag="P6L01_001991"
     CDS             complement(4555..4968)
                     /locus_tag="P6L01_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDP3031042.1"
                     /translation="MYKNILLGVDTQLKNEKALKEVSKLAGEGTVVTVLNAISEQDAQ
                     ASIKAGVHLNKLTEERSKRLEKTRKALEDYGIDYDQIIVRGNAKEELLKHANSGKYEI
                     VVLSNRKAEDKKKFVLGSVSHKVAKRATIPVLIVK"
     gene            5173..6471
                     /locus_tag="P6L01_001992"
     CDS             5173..6471
                     /locus_tag="P6L01_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDP3031043.1"
                     /translation="MTKHEQILDYIESLSIGSKISVRKIAKFLNVSEGTAYRAIKDAD
                     KMGMVATIDRVGTVRIEKRNRNEIEHLTFNEIVNIIDGQVLGGNKGITKMVSKFAIGA
                     MELKDILKYIGPKTLLIVGNREDVQIEALKRGTAILITGGFKPSNKVINFANEHDLPV
                     LSSSYDTFLVANIINKALFNQKIRKDILIVQDIMTPLDDLSVLFDTMKIADYKRMANR
                     TGHTRFPVVNESYKLVGIVTSREMINTKDDDEIDKVMTRNPIYVNAMSTVASCAHMMI
                     WEGIELIPVVSSNKKTVGVINRQDVLKSMQLLGRQPQMGETINDQIAKYITMNQDGIT
                     VEVSPLLINHYGTVSKAAFVSIIEETIQYEMRKFKKGNVMIENLNIVYIKTVPIESHI
                     TVRFGILDVGRNFAKIEVNMHSQNDKVASALVICQMFDEV"
BASE COUNT         2172 a         1013 c         1201 g         2251 t
ORIGIN      
        1 tttaaaataa tttttaaata aatattaagc gtctatctcc gctacaatta tatgtaggag
       61 gtagacgctt tttgtattgt ttgaaaatta actagtgtaa tgtctctttt cacgaatatc
      121 ttaaagaatt aagtagtcga agaagattat gaaaacatta ttaagtgagt tttatttata
      181 aacaataaaa aaccaagtgc agaaagttgc acttggttca taatgatcaa gtagttatct
      241 aatgatcatc tatatttaat tttgatattt ttcacgtaat tgagttgttg caacttgtgg
      301 ttggaagtct gctggtaact cttcagtacg aacaactaac acgtcacatg gcgcatgacg
      361 aacgatagat tctgatacag aaccaacaat aaatctttcc acggcattta agcctgatgt
      421 accactcata atcaagtctg cattaatttc atgtgcaagc tttttaggaa tgatagattt
      481 aggagagcca aactctagac gcgtttctac atctttaaca ccagcgttag tagctacttc
      541 tttataacca tttaataatt cttctgcaaa atgcttagat ttttcagtaa attgagcatc
      601 ataaacttca taagaagaat acgttcttga atcaattaca ttcacaattg ttaacttcgc
      661 atcgttacgt ttagcaacac caactgctct gttaaatgcc cattccgctt catgtgaacc
      721 gtcaactgcg attaaaatat ttttgtaagt aatcatagta agtgcctcct tgttctttct
      781 taattatatt ttaccacaat tttcggaaaa gtcagtgtaa taacctttac aattttgtgg
      841 atttttgatt gcgctttcaa aatgttcgcg ttaagatgat tatggaggtg gatggtatga
      901 aaattggtat accaagggag attaaaaata atgaaaatcg tgttggttta tcaccgagtg
      961 gtgtgcacgc tttagtagaa agtgggcata ctgttttagt ggaaacaaat gcgggttcag
     1021 gatcgttctt tgaagatgta gattacaaag aagcaggtgc tgagattgtt gctgaacaag
     1081 caaaagtttg ggatgtggat atggttatta aagttaaaga accacttgaa tctgaatatc
     1141 catattttaa agaagggctt gtattattca cttatcttca tttagcaaat gaagaaaaat
     1201 taacacaagc tttgatagat agaaaagtaa ttagtattgc atatgagact gtgcagttgc
     1261 cagaccgatc tttaccattg ttatcaccaa tgagtgaggt agcaggaaga atgtcagctc
     1321 aagttggcgc agagttccta caaaaactta atggtggtat gggaattcta ctaggtggtg
     1381 tcccaggagt acctaagggt aaagtaacta ttatcggtgg tggtcaagca ggaacaaatg
     1441 cagctaaaat tgcactagga ctaggtgcag atgttacgat tttagatgtt aatccaaagc
     1501 gtttacaaca attagatgat ttattcggtg gacgtgtaca tacaattatg tcaaatccgt
     1561 tgaatattga gttgtatgtt aaacaaagtg atttagtaat aggtgcagtt ttaattccag
     1621 gtgctaaagc gccaagactt gtaacagaag acatgattaa acaaatgaaa aatgggtcag
     1681 ttattattga cattgctatt gatcaaggcg gtatttttga aacaactgat aaaattacga
     1741 cacatgatga tcctacatat attaagcatg gtgtggttca ttatgcagtt gcaaatatgc
     1801 caggtgcagt accgcgtact tcgacgttag ctttaaataa tgctacgcta ccttatgcgc
     1861 tcatgctagc taataaaggg tatagagaag catttaaatc aaatcaacca ttatcattag
     1921 gtttaaatac ttacaaaggt cacgtaacca ataaaggcgt tgcagaggca tttgaaatgg
     1981 aatataaatc tgtagaagaa gcattacaat tataatttga atttgatgaa aagggaatgg
     2041 attacagcgt actaaagaat gacgttatca tctccttggt tattaaaatt aaataatgat
     2101 aaattaaagc tagaaacaat gcgaagaaat catcgaacat gaatgatatt gatttgattc
     2161 attacatgtt actgatgaac atacctgcat atgtgtttct agcttgtttg ttacatattg
     2221 catttagact tggataaaaa tttatgtctt aggcgctaca atgttatatt ggcagtgttt
     2281 gactgaatga gaatgtgctt gtaacaagct tttcattttt atcccactcc ttatttttcg
     2341 taatgtgtta atacttcata tccttcatta gtgacaagta tgtcatcttc aattcttaca
     2401 cctgcaacac ctggtacata aatacctggt tcgattgtaa taaccatgcc agcttctaac
     2461 aaattagaat tagtacttga aacatcttga tattcatgtt cttgtaatcc taaaccatga
     2521 cctaagcgat gagggaaata ttcaccataa cctttttctg aaataatatt tctagcgata
     2581 tgatcgatat cttttaaagg tattccaggt ttaattgctt ggattgcaga tgtttctgct
     2641 tctaatacaa tattataaat ttcttgtgct tctttgctag gttcaccaaa tttaatggta
     2701 cgtgtcatat cgctacaata atgctcataa attacaccta aatcaaatag tacatattca
     2761 ttgcttttta atctgcgatc tcctggtgtg ccatgaggtg atgcggcatg atctccaaat
     2821 aaaaccatcg tatcaaaact catttcattg acgccatatt gtttgatagt ttgctcaata
     2881 tggttgacta cttcacgttc agtcacacct tcttttaaat aagaaacacc tatttcgata
     2941 cacttatctg ctaactcagc agctttacgt attttgctaa tttcatcttc ggatttaata
     3001 tttcttaatt gtttgattgt taaatcaaca tctccgaatg aattgacatt gaaaccagag
     3061 attaattgtt tttggcgtgc tactgttaag tgctcgcttt caattagtaa tttattgatt
     3121 gtttgaggat aaagtgaaaa aggattttca gtgtctaaat atccaacgat ttcacctgtg
     3181 aaaggtgatg ctttgacttc ttcgacttcc atttttggac aaaatagtac ttgtttacca
     3241 tctttcttaa tcaataatgc aaataatctt tcatggggtt cgctacggta tccagtaaaa
     3301 taatatacat tcaacggtgt tgtaatccat gctgcatcag cttgttgatt gttcagttcg
     3361 tctattattt ttgatatttt tgtcatttca taaacactcc tcaacataat attacttcca
     3421 ttttatgcca attattatta gatttcaaaa tcgagctaat acattaacta gattttcaat
     3481 ttttaaaatt tgagaatttg ttataattaa gtggcaaaag ggtactaaat ttatatagca
     3541 attgatagga ggatgctcaa atgaagttat catttcatgg tcaatcaacg atttatttag
     3601 aagggaacaa caaaaaggtt atcgtagatc cgtttatttc aaataaccct aagtgtgatt
     3661 tgaatattga aacagtgcag gtagactata tcgttttaac gcacggtcat tttgatcatt
     3721 ttggtgatgt tgtggaacta gcaaagaaaa caggagcaac agtaattgga agtgcagaaa
     3781 tggctgacta tctttcttca tatcacggtg ttgaaaatgt tcatggtatg aatattggtg
     3841 gtaaagcaaa ctttgatttc ggaagcgtta aatttgttca agcatttcat agttcaagtt
     3901 ttacacatga aaatggtatt ccagtttatt taggtatgcc aatgggtatc gtgtttgaag
     3961 ttgaaggaaa aacaatttat catactggtg atacaggtct gtttagtgac atgtcactta
     4021 ttgcgaaacg tcatccagtt gatgtatgtt ttgtaccaat tggagataat tttacgatgg
     4081 gtattgatga tgcaagttat gctattaatg aatttattaa accaaaaatt tctgtaccga
     4141 ttcattatga tacattccca ttaatcgaac aagatccaca acaatttaaa gatgctgtta
     4201 atgtagggga cgtgcaaatt ttaaaaccag gtgaatctgt tcagttttaa atagaatata
     4261 gattttacac taaaaaacac ctcgctaatc tatgtggaag ttatcgtctg catattttta
     4321 gtgggtgttt ttatattcta ctgattcagt tacgacgtgg acgggggaca tcagtggttc
     4381 tttatcattt ctgacctact ctcgtaattt tagttttata caattagaca ttgattataa
     4441 atttactacg atataaaaaa tccagaacca cagtcataat catttcttgt gtttactcag
     4501 tcgtttgaga ctgcatcatg attgaaagat tatttgtgat tctggataaa aattttattt
     4561 aacgattaat acagggatag tcgcacgttt tgctactttg tggctgacac ttccaagtac
     4621 aaatttcttt ttgtcttctg ctttacggtt acttaaaaca acaatttcgt atttaccgct
     4681 attagcatgt tttaatagtt cttcttttgc attaccacga acaattattt ggtcataatc
     4741 aataccataa tcttctaaag ctttgcgtgt tttttccaat cgcttgcttc gttcttcagt
     4801 aagtttgttt aaatgaacac ctgctttaat tgatgcttga gcatcttgtt cgctgattgc
     4861 gtttaaaact gttacgactg taccttcgcc agctaattta gacacttctt ttagtgcttt
     4921 ttcatttttt aactgagtgt ctacaccaag taatatattt ttatacatta gtacatccct
     4981 cctcacttac attctaataa ataataatgg atttttccat ttaaaattaa agattttgtg
     5041 atctttgtgt ttgttgtata ttttcaaaaa ataaaatagg tgaaacgatc attatttcat
     5101 ttcacttcaa tgctttttat ccaaaataaa gttataatgt agttaatact gttcgaaaag
     5161 aagaggtaaa gaatgacaaa acatgaacaa attttagatt atattgaatc gttatctata
     5221 ggctctaaaa tttcggtgcg taaaatcgct aaatttttaa acgtatctga agggacagca
     5281 tatcgtgcaa ttaaagatgc tgataaaatg ggcatggtcg caacgataga tcgtgttggt
     5341 acggttcgaa tcgaaaaacg taatcgtaat gaaattgaac atttaacttt taatgaaatt
     5401 gtcaatatta ttgatggcca agtgttaggt ggcaataaag gtatcactaa gatggtatct
     5461 aaatttgcaa ttggagcaat ggaactcaaa gatattttaa aatatattgg tccaaagaca
     5521 ttattgattg tcggaaatcg cgaagatgtg cagattgaag ctttaaaaag aggtacggct
     5581 atattaatta ctggtggatt taagccatct aataaagtta ttaattttgc gaatgaacat
     5641 gatttacctg ttctatcatc aagttatgat acattcctag ttgcaaatat tatcaacaaa
     5701 gcacttttta atcagaaaat acgaaaagat atcttaatag ttcaagatat aatgacgcca
     5761 ttagatgatt tgtctgtgct gtttgatacg atgaaaatag cagattataa gcgtatggcg
     5821 aatcgaactg gccatacaag atttccggta gtcaatgagt cgtataaatt agtaggtatt
     5881 gtaacaagtc gagagatgat taatacaaag gacgacgacg aaatagataa agtgatgacg
     5941 cgaaacccta tttatgtaaa tgctatgagt acagtagcaa gttgtgcgca tatgatgatt
     6001 tgggaaggta ttgaattgat tcctgtcgtt tcgagtaata aaaagacagt gggtgtgatt
     6061 aatagacaag atgttttaaa gtctatgcaa cttttaggaa gacagcctca gatgggtgaa
     6121 acaattaacg atcaaatcgc caaatatata acaatgaatc aagatggaat tacagtagaa
     6181 gtttcaccac ttttaatcaa tcattacgga acagtaagta aagcggcatt tgtaagtatt
     6241 atagaagaaa caatacaata tgaaatgcgt aaatttaaaa aaggtaatgt catgatagag
     6301 aacttaaata tcgtgtatat taaaacagta cctattgaat cacatataac agtgcgcttt
     6361 ggcatactag acgtcggaag aaacttcgcc aaaatagaag tgaacatgca tagtcaaaac
     6421 gacaaagtag cgagcgctct cgtgatatgc caaatgtttg acgaagtata ggaacttagc
     6481 agagaaaata ggcataagat agatttcgca caaacgttgg agcccaactt acattgtttg
     6541 tagaatttct tttcgaaatt ctttgtgttg gggccccacc ccaacttgca tcgtctgtag
     6601 aaattggggg tccaatttca ctgtgttggg gcccctg
//
LOCUS       DAOBBH010000137         6611 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.137, whole genome shotgun sequence.
ACCESSION   DAOBBH010000137 DAOBBH010000000
VERSION     DAOBBH010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6611
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.137"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..646
                     /locus_tag="P6L01_001993"
     CDS             <1..646
                     /locus_tag="P6L01_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDP3031044.1"
                     /translation="IMRLAKIQLWFLIKGLTPIFFFLIFTLMMHIFLTKGGYVLVEWH
                     GITIETNGILEGLYISLRLIGIVMIATIMTLSTSPIDLTDAFERLLAPLKMFKLPVHQ
                     LSMIMSIALRFIPTLMDELDKIILAQKSRGSEISSGNIATRIKSFIPLLVPLFISAFQ
                     RAEELAVAMEVRGYDANVKRTSYRQLKWQLRDTISLTMIIPIAIILFVLKYSGV"
     gene            651..1454
                     /gene="truA"
                     /locus_tag="P6L01_001994"
     CDS             651..1454
                     /gene="truA"
                     /locus_tag="P6L01_001994"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDP3031045.1"
                     /translation="MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRI
                     HPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRY
                     DCVGKRYRYKVYQAQHRDPFQSGLKTFIPETLDLDKMNRAAQQFIGTHDFTGFCSQKT
                     EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEISDVPKL
                     LESKNRKNVPFTAPAEGLYLEKIYLDENELLKDFGNDIKIHRKKSLQND"
     gene            1694..2131
                     /gene="rplM"
                     /locus_tag="P6L01_001995"
     CDS             1694..2131
                     /gene="rplM"
                     /locus_tag="P6L01_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDP3031046.1"
                     /translation="MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKVT
                     YTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSNHPGGIKSITAGELRRTNPERLI
                     ENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG"
     gene            2145..2543
                     /gene="rpsI"
                     /locus_tag="P6L01_001996"
     CDS             2145..2543
                     /gene="rpsI"
                     /locus_tag="P6L01_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDP3031047.1"
                     /translation="MTLAQVEYRGTGRRKNSVARVRLVPGEGNITVNNRDVREYLPFE
                     SLILDLNQPFDVTETKGNYDVLVNVHGGGFTGQAQAIRHGIARALLEADPEYRGSLKR
                     AGLLTRDPRMKERKKPGLKAARRSPQFSKR"
     gene            complement(2721..3668)
                     /locus_tag="P6L01_001997"
     CDS             complement(2721..3668)
                     /locus_tag="P6L01_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031048.1"
                     /translation="MKKSIYILPLAYIVIESPLQIGNVILTPSLKVIESGHFHTDFSL
                     ISMFNKKEFLSILSTSQLKEDALVIFKDDFFEPTTVDQNYYFINKLSQKADRTLDIIR
                     INENNFGVPEQLPGIPGITSNGWQVAEQLTDHKLRTINGKVMFMIREGIGLYPIDLSS
                     NIYEDKLYNCLYVTSNEINLLFREAIHRVSESMYMNNLNTSFVYLLSTLEMLSSREYL
                     NFKKTKSKILPFISYSKYQYHKCSEYLRNISENLRTEIIHNGKSIFDLYDNYEDVYKL
                     LNWIKLIIIEYISNIVKKNIQTFDDLEKERKKIINSLGI"
     gene            4513..4839
                     /locus_tag="P6L01_001998"
     CDS             4513..4839
                     /locus_tag="P6L01_001998"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDP3031049.1"
                     /translation="MKVKLRLTCVYSAFRAYVLKHVVFNRYFMKGYQQMSPKGTTRIE
                     TKTGHQAQFDWKEGINFKTNDNQIVSLNIGVLLLSYSRFVIMKVTMNKSSDVLFNLFT
                     QAFELM"
     gene            5323..6041
                     /locus_tag="P6L01_001999"
                     /pseudo
     CDS             5323..6041
                     /locus_tag="P6L01_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066123.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         2416 a          953 c         1082 g         2160 t
ORIGIN      
        1 cattatgaga ttagcaaaaa ttcaattatg gttcctaatc aaaggtttaa caccaatatt
       61 tttcttttta atcttcacat taatgatgca tatattttta actaaaggtg gatatgtatt
      121 agttgaatgg catggcatca cgattgaaac taatggtatt ttagaagggc tatatatatc
      181 attgcgttta attgggattg tgatgattgc aacaattatg acactatcta caagtccaat
      241 tgatttaaca gatgcgtttg aaagattact tgcaccacta aagatgttta aattaccagt
      301 tcatcaatta agtatgataa tgtccattgc gttacgattc atcccgacgt taatggatga
      361 gttagataaa attattttgg cgcaaaagtc gcgtggttct gaaataagtt cagggaacat
      421 tgcaacacgt attaaatcat ttattccatt actagtgcca ctattcatct cagctttcca
      481 acgcgccgaa gaattagcgg tcgcaatgga agttaggggt tatgatgcca atgttaagag
      541 aacgagctac agacagctta aatggcaatt gagagatacg atatctttaa ccatgattat
      601 cccaattgca attattttat tcgtattaaa atattcagga gtgtaaattc atgcgtatat
      661 tagtagaaat tgcgtatcaa ggaaataatt ttctaggctt tcaaattcaa cagaatggac
      721 gtacggtaca gcaacaattt gaaaagctat tacaacgcat gcataaaaga catgtgagaa
      781 tccatccttc aagtaggact gatagaggtg tacatgcgat acagcagtac tttcatttcg
      841 acacagagct gaatatacct atgtcacaat ggcaatatgc aatgaaccga acgttaccag
      901 atgatattta tgtcaataat gtagttacag tcgatgacga ttttcattgt cgttacgatt
      961 gtgtcggtaa acgctatcga tataaagtat atcaagcaca acaccgcgat ccatttcaaa
     1021 gtggtttgaa aacatttatt cctgaaacgt tggatttaga caaaatgaac agagcggcgc
     1081 aacaattcat aggtacacat gattttacgg ggttttgttc gcaaaaaact gaagtagaaa
     1141 gcaaagttag aacattatac caaagtgaaa tcgtaaagac agatgatggc tttgattata
     1201 ttgtcactgg atctggattt ttatataaca tggtgcgtgt cttagttgca tttttaatag
     1261 aagttggaaa agggcgacat gaaatttctg atgtgccgaa gctacttgaa tcaaagaatc
     1321 gtaagaatgt tcctttcact gcaccggctg aaggactata tttagagaag atttatttag
     1381 atgaaaatga gttattaaaa gactttggca acgatataaa aatacatcgc aaaaaatcat
     1441 tacaaaatga ctgattgcca ttgacaaaaa gcttgtgaaa ttataagatt attaacggta
     1501 ttgttttata tccaccccac gataagcccc ggaaacttat tgtgttacaa gatatataag
     1561 cagaaacgaa caacagttaa caaaataaat gaaattaaac gttttaaaaa tgaaacaaat
     1621 gaaatcatct attaggttat gaaactgttt atagcttgaa tagaagcatt tattttttag
     1681 gaggacaatt attatgcgtc aaacatttat ggcaaatgaa tcaaacattg agcgcaaatg
     1741 gtatgttatc gatgctgaag gccaaacatt aggtcgttta tcatcagaag tagcatctat
     1801 cttacgcggt aaaaataaag taacttacac accacacgtt gatactggtg attatgtaat
     1861 cgttattaat gcatcaaaaa tcgaatttac tggtaacaaa gaaactgaca aagtttacta
     1921 ccgtcactca aatcatccag gtggtatcaa atcaatcact gctggtgaat taagaagaac
     1981 taacccagaa cgtttaattg aaaactcaat taaaggtatg ttaccaagca ctcgtttagg
     2041 cgaaaaacaa ggtaaaaaat tatttgtata tggtggcgct gaacatccac acgctgcaca
     2101 acaaccagaa aactacgaat tacgtggtta attagaagga ggaaatgact ttggcacaag
     2161 ttgaatatag aggcacaggc cgtcgtaaaa actcagtagc acgtgtacgt ttagtaccag
     2221 gtgaaggtaa catcacagtt aataaccgtg acgtacgcga atacttacca ttcgaatcat
     2281 taattttaga cttaaaccaa ccatttgatg taactgaaac taaaggtaac tatgatgttt
     2341 tagttaacgt tcatggtggt ggtttcactg gacaagctca agctatccgt cacggaatcg
     2401 ctcgtgcatt attagaagca gatcctgaat acagaggttc tttaaaacgc gctggattac
     2461 ttactcgtga cccacgtatg aaagaacgta aaaaaccagg tcttaaagca gctcgtcgtt
     2521 cacctcaatt ctcaaaacgt taattgtcgg acgatatata caaaacacct cgatattatg
     2581 tcgaggtgtt tttttgcgtt tgtgtgtttt aaaattattt ggctctatat caaattggac
     2641 tgatgcattc aatatcggaa gttaggcaac taaacattgc ttaacttcct ttttattttt
     2701 tggagcgtaa agttttgaac ctaaattcca agagaattaa taattttttt cctttctttt
     2761 tcgaggtcat caaaagtttg tatattcttt ttaactatgt tacttatata ttcaataatt
     2821 attaatttaa tccaatttaa tagcttgtac acatcctcat aattatcata taaatcaaat
     2881 atacttttac cattgtgaat aatttcagtt cttagatttt cactaatatt tcttagatac
     2941 tcactgcact tatgatactg atatttagaa taagaaataa aaggaaggat tttagattta
     3001 gtttttttaa agtttaaata ttcacgactt gatagcattt ctaatgtact taaaagataa
     3061 acaaatgatg tatttaaatt attcatgtac attgattcag aaactctgtg gatagcttct
     3121 ctgaaaagta aattaatttc atttgaagta acataaagac aattatataa tttatcttca
     3181 taaatattag aagataagtc aattggatat aatcctattc cttctcgaat cataaacatg
     3241 acttttccat taattgttct aagtttgtgg tcagtcaatt gttcagctac ttgccaacca
     3301 tttgaggtaa tacctggtat acctggtaat tgttcaggaa caccaaaatt attttcgttt
     3361 attcttataa tatctagagt tctgtcggct ttttgactta acttattaat aaaataataa
     3421 ttttggtcta ccgtggtagg ttcaaagaaa tcgtccttaa aaataactag tgcatcttct
     3481 tttaattgcg aagtagataa aatagataaa aactcttttt tgttaaacat tgatattagt
     3541 gaaaagtctg tatgaaaatg acctgattca attactttta aagatggggt taagataaca
     3601 ttgccaattt gtaaaggtga ctcaataact atataagcta atggtaagat ataaatactc
     3661 tttttcatta taaaatcctc ctaaaaatta atatgttttt gaaattcatc tgccaatttt
     3721 tctctagatg attttgtaat gtgtttataa atgtccatag tcgtctttat atcactatga
     3781 cctaatctag ttttacttct ttaagttgtt gcacctgatt cgaataatag tgaagcacga
     3841 gtatgcctga agctatgaac gtgtatctta tgaagatttg aatatcattt aaagaatacc
     3901 tctaattcgt cattaggcat cgatacacgc atgtacttca tttagtctac tatgtatggt
     3961 atgttgaaaa gatatgttgc tctttatcac tagagactga aaatccatat tcttcatttt
     4021 gtagattttg ttcttctttc cattgtaaaa gtttatttat agtttgaata ccaatatcta
     4081 tccatctttg atactcacat ttttttagtg gttgcaatac atgtttattt ttcactggaa
     4141 attataattt aataatgtac acttatgatt gtttaaacat gtaaaatttt taaaaaccta
     4201 gagataattt tattgaggcg aacaacaatg aaattatctt tagacataaa tatggatttt
     4261 taagttatta ctcttacaga tttaccaaaa ttaaaaattg ttaagcagaa cttaatcatg
     4321 aaataaacaa aagtgaaata gcaagacata tgggggatga tagaagaaca gttgataaat
     4381 aattaaatgg ttttgagccg accaagaaga ggaaccgtca gtctattatt gacaagtatt
     4441 atccaatcat ccaaaagtta ctttcacact agtgaataat agttctacta taatggtgta
     4501 ttttgacaat atttgaaggt taaacttaga ttaacctgtg tctattctgc atttagagct
     4561 tatgtcctta agcatgttgt atttaatcgt tattttatga aaggttatca acagatgtcg
     4621 cctaaaggta caacgagaat cgagacaaaa accggtcatc aagcacagtt tgactggaaa
     4681 gaaggcatta actttaaaac gaatgataat caaatagttt cactaaatat aggcgtgtta
     4741 ctactttctt actcccgttt tgtaatcatg aaagtgacaa tgaataaatc gagtgatgtt
     4801 ttatttaatt tatttacaca agcgtttgag ctaatgtgag atgtgcctga tgtacttgta
     4861 actgatagta ttatatttag aagaggttcc taagtttatt tctaatctta aagagcgact
     4921 gaactactca gttcatgtga gtacaggtaa aatgcctatt attgccttag aaaaagaaaa
     4981 agttcttaca atcactaccg aatgtgaatg taagaaactc atataaagtg aaacataaat
     5041 atttgaaagt caaccgctct aatatgatta catataagtc caatcaatat tcagcatcag
     5101 ctgaatattg tgggaaaaca gttgaagttc aagtttatga tcaaaaacaa cacgtttaat
     5161 ataagactaa attgatttgt gaacatctca tcacacaacg taaattaaat tatcaaaaag
     5221 aacattattt agaatcactg ccatcctctt ttgggaatag agaaaaggat gatattaatc
     5281 agattacgat agataattta aatgcgattg gatagtttta tgatgaataa ttcaacgaat
     5341 tataagcgct taaaagcaaa tttagaatat ttgaaaatga atcagatgat taaccatctt
     5401 gatgatgtta tcgattttat aaacacaacc tgtcattcat agacacatta attaaattaa
     5461 gcgattacaa aatcaaaatt aaggagaaaa atttgattga atcgatggta aaggttatgg
     5521 cgttaccgtt taaaaagaaa tatcagcctt tgactttaat tttcaaccga gcatcaatca
     5581 acaagatgtt ttggatttta cggatttacg ttttatagga aaatatgaaa acatagtctt
     5641 tttagactca aatgtcgtta gtaaaaatag aataagtact tactttatta agtgtcatca
     5701 tttgaaagag aatttaagaa aagcaaaact tgagaacagc ttagaaaata gattgaagca
     5761 ctatagcaag tataagttat taatcattga tgagattggg tacttaccta ttggaagtga
     5821 agctgccaaa atattcttcc aaattaatag atttaaggta tgagaagaaa agcacaatat
     5881 ttaccacaaa tatcaatttc aacttataaa atgaaatatt tgatgatccc aaaatcgcaa
     5941 atgcgatttt ggataggata ttgcatcatt caagtgtagt aaaaatcaca ggaaaatcgt
     6001 atagattaaa aaatcattca cctaaaaaga aaattactta gcaaacaatt ttgtacattt
     6061 ctatacaatc atttatgtag attttaatat tgacatttac atccaactaa atgtagacga
     6121 ttcacagaca accgatataa ttaatttgtc catccatttt aggtggcatt ttatcatgag
     6181 gagtatagtt ttgattgcaa aaaaacaaat attagaacat ttatcgtgaa gaaaaaatga
     6241 tatacttgaa gaaaagtata aatataaaaa gtgaagatat ttataagagg agaaagctaa
     6301 tggctaatac aaaacaaatc cattaaatca acaagaactt agaaatgcaa tctagtcagg
     6361 tccatttata acaaaagcaa agtatgaatt ttctaattct atgaatgcta atcagcaaaa
     6421 aatggcaaag ttatattaaa ggaattccaa atagataaga aatattagaa gaagctctaa
     6481 atgggggaag taaaggtgat attaatggat acatgtcaag acatcataat gatataaatg
     6541 atttaaaaac atactttaac tcagttatag attggatatc gagcattttt attaatgtag
     6601 aaaaagaaat g
//
LOCUS       DAOBBH010000138         6490 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.138, whole genome shotgun sequence.
ACCESSION   DAOBBH010000138 DAOBBH010000000
VERSION     DAOBBH010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.138"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            71..730
                     /gene="arlR"
                     /locus_tag="P6L01_002000"
     CDS             71..730
                     /gene="arlR"
                     /locus_tag="P6L01_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor ArlR"
                     /protein_id="HDP3031050.1"
                     /translation="MTQILIVEDEQNLARFLELELTHENYNVDTEYDGQDGLDKALSH
                     YYDLIILDLMLPSINGLEICRKIRQQQSTPIIIITAKSDTYDKVAGLDYGADDYIVKP
                     FDIEELLARIRAILRRQPQKDIIDVNGITIDKNAFKVTVNGAEIELTKTEYDLLYLLA
                     ENKNHVMQREQILNHVWGYNSEVETNVVDVYIRYLRNKLKPYDRDKMIETVRGVGYVI
                     R"
     gene            727..2082
                     /gene="arlS"
                     /locus_tag="P6L01_002001"
     CDS             727..2082
                     /gene="arlS"
                     /locus_tag="P6L01_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase ArlS"
                     /protein_id="HDP3031051.1"
                     /translation="MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDD
                     AERSSSDINNLFHSKPVKDISALDLNASLGNFQEIIIYDEHNNKLFETSNDNTVRVEP
                     GYEHRYFDRVIKKRYKGIEYLIIKEPITTQDFKGYSLLIHSLENYDNIVKSLYIIALA
                     FGVIATIITATISYVFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANT
                     FNEMMSQIEESFNQQRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNIS
                     IEEMNRIIKLVEELLELTKGDVNDISSEAQTVHINDEIRSRIHSLKQLHPDYQFDTDL
                     TSKNLEIKMKPHQFEQLFLIFIDNAIKYDVKNKKIKVKTRLKNKQKIIEITDHGIGIP
                     EEDQDFIFDRFYRVDKSRSRSQGGNGLGLSIAQKIIQLNGGSIKIKSEINKGTTFKII
                     F"
     gene            2366..5164
                     /locus_tag="P6L01_002002"
     CDS             2366..5164
                     /locus_tag="P6L01_002002"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDP3031052.1"
                     /translation="MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLF
                     STIKNDDSIVPALKSTSSQNSDGTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPK
                     LEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNT
                     ERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALV
                     PMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPED
                     GSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDD
                     TQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIG
                     FTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDL
                     VGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGVISEDEMHSFIEQVQ
                     KELRQAHDKINKADKMDNPDMEKPAELALPLQADEQSFTFDHLKEINDALLTYPDGFN
                     ILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHR
                     HAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEA
                     QYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAA
                     ENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSG
                     GFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIE
                     ALLAQLPNLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYHGRIQRAAPAEGDG
                     EIHKLVQNKIIENALKNN"
     gene            5178..6446
                     /gene="sucB"
                     /locus_tag="P6L01_002003"
     CDS             5178..6446
                     /gene="sucB"
                     /locus_tag="P6L01_002003"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDP3031053.1"
                     /translation="MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVN
                     VEVVSEEAGVLSEQLASEGDTVEVGQAIAIIGEGSGNASKENSNDNTPQQNEETNNKK
                     EETTNNSVDKAEVNQANDDNQQRINATPSARRYARENGVNLAEVSPKTNDVVRKEDID
                     KKQQAPASTQTTQQAPAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLT
                     TFNEVDMTNVMELRKRKKEQFMKDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDD
                     MITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVKAREKKLGLDDMVN
                     GSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYD
                     HRIIDGKEAVGFLKTIKELIENPEDLLLES"
BASE COUNT         2441 a          961 c         1203 g         1885 t
ORIGIN      
        1 attgtataga aaaatacaaa atttaaaata tattacctta ttagaaaaag tcgtaatatg
       61 aggtgtacaa atgacgcaaa ttttaatagt agaagatgaa caaaacttag caagatttct
      121 tgaattggaa ctcacacatg aaaattacaa tgtggacaca gagtatgatg gacaagacgg
      181 tttagataaa gcgcttagcc attactatga tttaatcata ttagatttaa tgttgccgtc
      241 aattaatggc ttagaaattt gtcgcaaaat tagacaacaa caatctacac ctatcattat
      301 aattacagcg aaaagtgata cgtatgacaa agttgctggg cttgattacg gtgcagacga
      361 ttatatagtt aagccgtttg atattgaaga acttttagca agaattcgtg caattttacg
      421 tcgtcagcca caaaaggata ttatcgatgt caacggtatt acaattgata agaacgcttt
      481 taaagtgacg gtaaatggcg cagaaattga attaacaaaa acagagtatg atttactata
      541 tcttctagct gaaaataaaa accatgttat gcaacgggaa caaattttaa atcatgtatg
      601 gggttataat agtgaagtag aaacaaatgt cgtagatgtt tatataagat atttacgaaa
      661 caagttaaaa ccatacgatc gtgacaaaat gattgaaaca gttcgtggcg ttgggtatgt
      721 gatacgatga caaaacgtaa attgcgcaat aactggatta ttgttaccac gatgattacg
      781 tttgtcacga tatttttgtt ttgtttaatt attatttttt tcttgaaaga tacactgcat
      841 aatagtgagc ttgatgatgc agaacgaagc tcaagcgata ttaataattt atttcattct
      901 aagcctgtta aagatatatc tgcattagac ttgaatgcat ctttaggtaa ttttcaagag
      961 ataattattt atgatgagca taataataaa ttatttgaga catcgaatga taacacagtg
     1021 agagttgaac caggttatga acaccgttat tttgaccgcg taataaaaaa acgctataaa
     1081 ggcattgaat atttaattat taaagaacca attacaacgc aagatttcaa agggtatagc
     1141 ttgttaattc attcactaga aaattatgat aacatcgtaa aatcattgta tatcattgcg
     1201 ctggcatttg gagtgattgc aacaattata actgccacaa tcagttatgt attttcaaca
     1261 caaattacta aaccgcttgt cagtttatca aataaaatga ttgagattcg acgagatggt
     1321 tttcaaaata aattgcaatt aaatacaaat tatgaagaaa tagataattt agcaaatacg
     1381 tttaatgaga tgatgagcca aattgaagaa tcatttaatc aacaaagaca atttgttgaa
     1441 gatgcgtcac atgaattacg aacaccatta caaattattc aaggtcattt aaatttgatt
     1501 cagcgatggg gaaaaaaaga cccagcagta ttagaagaat cgttaaatat ttctattgaa
     1561 gaaatgaatc gtatcataaa attagtcgaa gaattacttg aattgactaa aggagatgta
     1621 aatgacattt cttctgaagc gcagaccgtg catattaatg atgaaattcg ctcgcgaata
     1681 cactcattaa aacaattgca tcctgattat caatttgata cggatctgac atctaaaaat
     1741 ctagaaatta aaatgaaacc tcatcaattc gaacaattat ttttaatctt tattgataat
     1801 gcaatcaaat atgatgtgaa gaataagaaa attaaagtta agacaaggtt aaaaaataag
     1861 caaaaaataa ttgaaattac agatcatgga attggtattc cagaggaaga tcaagatttc
     1921 atttttgatc gcttttatcg agtggataaa tctcgttcaa gaagtcaagg cggtaatgga
     1981 ctcggattat ctattgctca aaaaatcatt caattaaacg gaggatcgat taaaattaaa
     2041 agtgaaatta ataaaggaac aacgtttaaa atcatatttt aatcatgtct gagacgtcaa
     2101 tcaaagtcat aggatcaatt ttttaagtac acattagctg tgactaatgt ataagaacaa
     2161 ctataaaaca aataaacagt ggttctttat catttctgtt gtactcccaa aatttacaat
     2221 aaaatacatc tataaaccta gaagaatcaa cgcttttgtt gattcttctt tttagcagat
     2281 aaataggtaa atctacttta acaaataact aaatagtgat attattacat tgtaagcgtt
     2341 tcaacatttt tgtggagggt gtaaaatgac taacgaaaga aaagaagttt cagaggctcc
     2401 tgtaaacttc ggtgcgaatt taggtctaat gttagatcta tatgatgact ttttacaaga
     2461 tccatcatct gtaccagaag atttacaagt cttattcagc acaattaaga atgatgactc
     2521 aattgtacca gctttaaaaa gtacaagtag tcaaaatagc gacggcacaa ttaagcgtgt
     2581 catgcgtcta attgataata ttcgccaata cgggcatctt aaagccgata tttatcctgt
     2641 aaatcctcca aaaaggaaac atgtacctaa attagagatt gaagactttg atttagatca
     2701 acagactttg gaaggtatat cagcaggaat tgtttcagat cactttgccg acatttatga
     2761 taatgcttat gaagcaattt taagaatgga aaaacgttac aaaggaccaa ttgcatttga
     2821 gtatacacat attaataaca ataccgaacg tggttggtta aaaagaagaa ttgaaacgcc
     2881 atataaagta acgttaaata ataacgaaaa aagggcacta ttcaaacaat tagcgtatgt
     2941 tgaagggttt gaaaaatatc ttcataaaaa cttcgttggt gcaaagcgtt tttcaattga
     3001 aggggtagac gcacttgtac cgatgttaca acgtactatt acgattgctg cgaaagaagg
     3061 tattaaaaat atacaaatag gcatggctca ccgtggacgt ttaaacgttt taacgcatgt
     3121 cttagaaaaa ccgtacgaaa tgatgatttc agaatttatg catacagatc caatgaaatt
     3181 cttacctgaa gatggtagct tgcagttaac tgctggatgg actggtgatg tgaaatatca
     3241 ccttggtggc attaaaacta ctgattcata cggtacaatg cagcgtattg cactggctaa
     3301 caatccaagt cacttggaaa ttgttgcacc tgttgttgag gggcgtacga gagcagcaca
     3361 agatgataca caacgagctg gggctccgac gactgatcat cataaagcaa tgccaattat
     3421 tatacatggc gatgctgctt atcctggtca aggaattaac ttcgaaacaa tgaacttagg
     3481 aaacttgaaa ggctattcta cgggtggttc attgcatatt attactaaca atagaattgg
     3541 atttactaca gaaccaattg atgcacgttc aacaacttat tctacagatg tggcaaaagg
     3601 ttatgatgtg ccaatattcc atgtcaatgc tgatgacgtt gaagctacta ttgaagcaat
     3661 tgatattgca atggaattta gaaaagagtt tcataaagac gtcgttattg atttagtagg
     3721 ttatcgtcgt ttcggacata acgaaatgga tgaaccatca attactaatc cagttcctta
     3781 tcagaatatt cgcaaacatg actctgttga atatgtgttt ggtaaaaagc ttgttaatga
     3841 aggtgtcatt tcagaagatg aaatgcattc atttatagaa caagtccaaa aggaactaag
     3901 acaagctcat gataaaatta ataaagctga taaaatggat aatccagata tggaaaaacc
     3961 tgcagaactt gcattaccgt tacaagcaga cgaacaatca tttacgtttg atcatttgaa
     4021 agaaataaat gatgcattgt taacatatcc ggatggcttt aacattttga aaaagttaaa
     4081 caaagttctt gagaagcgtc atgagccgtt taataaagaa gatggcttag ttgattgggc
     4141 acaagcagaa caacttgcat ttgcgacaat tttacaagat ggtacaccga ttcgcttaac
     4201 tggtcaagat agtgaacgtg gtacattcag tcataggcat gccgtgttac atgatgagca
     4261 aacaggtgaa acatatacac ctttacatca tgttcctgat caaaaagcga catttgatat
     4321 acacaattct ccgctttcag aagcagcagt agttggtttt gaatacggct ataatgtgga
     4381 aaacaaaaaa agcttcaata tttgggaagc gcaatatggt gattttgcaa atatgtcaca
     4441 aatgattttt gacaacttct tattcagttc tcgctcaaaa tggggagaac gttcaggatt
     4501 aacattattc ttacctcatg catatgaggg tcaaggacct gaacattcat cagcaagatt
     4561 agagcgattt ttacaattag ctgctgaaaa taattgcaca gttgtcaact tatctagttc
     4621 aagtaattat ttccacttat tgcgtgcaca agcagctagt ttagattctg aacaaatgcg
     4681 accattggtt gttatgtcac caaaaagctt attgagaaat aaaacagttg ccaaaccaat
     4741 tgatgagttt acttctggtg gatttgagcc aattttgaca gaatcatatc aagcggataa
     4801 ggttacaaaa gttattttag caactggtaa aatgttcatt gatttaaaag aagcattagc
     4861 taaaaatcca gacgaatccg tgttactcgt tgcgattgaa agattgtatc cattcccaga
     4921 ggaagagatt gaagcattac tagcacaatt gccaaacctt gaagaagtgt catgggtaca
     4981 agaagaacct aaaaatcaag gtgcatggtt atatgtctat ccatatgtta aagtgctagt
     5041 tgcagataaa tatgatttaa gttatcatgg cagaattcaa agggctgctc cagctgaagg
     5101 cgatggagaa attcataaac ttgttcaaaa taaaattata gaaaatgcat taaaaaataa
     5161 ctagggggaa ataagtcatg ccagaggtta aagttccaga attagcagaa tctattacag
     5221 aaggtaccat tgcagaatgg ttgaaaaacg taggggatag cgtagaaaaa ggtgaagcta
     5281 ttcttgaatt agaaactgat aaagttaatg tcgaagttgt atctgaagaa gcaggtgtat
     5341 tatctgaaca acttgcaagt gaaggcgaca ctgtagaagt tggacaagca attgctatca
     5401 tcggcgaagg tagtggcaat gcttctaaag aaaatagtaa cgacaatact ccacaacaaa
     5461 atgaagaaac aaataataaa aaagaagaaa caacaaataa ttcggtagat aaagctgaag
     5521 tcaatcaagc aaatgatgac aatcagcaac gtattaatgc tacgccttct gcgcgtcgat
     5581 atgctcgtga aaatggtgtg aatcttgctg aagtaagtcc gaaaacaaat gatgtggttc
     5641 gtaaagaaga tattgataag aaacaacagg caccggcatc aacacaaaca acacaacaag
     5701 cacctgcaaa agaagagaaa aaatacaatc aatatcctac aaaaccagtg attcgtgaaa
     5761 aaatgtcacg cagaaagaaa acagctgcca aaaaattatt agaggtatct aataatacag
     5821 ctatgttaac aacatttaac gaagttgaca tgacaaatgt tatggaattg cgtaaacgta
     5881 agaaagaaca atttatgaaa gatcatgatg gtactaaatt aggatttatg tcattcttta
     5941 ctaaagcttc tgtagcagct ttgaaaaagt atccagaagt taatgcagaa atcgatggcg
     6001 acgacatgat tacgaaacaa tattatgata ttggtgtagc tgtttctaca gatgatggat
     6061 tattagtacc atttgtaaga gattgtgata aaaagaattt tgcagaaatc gaagcagaaa
     6121 ttgctaattt agcagttaaa gcacgagaga aaaaacttgg cttagatgat atggttaatg
     6181 gttcatttac gattacaaat ggcggtattt ttggatcaat gatgagtacg ccaattatca
     6241 atggtaatca agctgcaatc ttaggcatgc attcaattat tacaagacca attgcgattg
     6301 atcaagatac aatcgaaaat cgtccaatga tgtatattgc attaagctat gatcatagaa
     6361 ttattgacgg taaagaagca gttggattct taaaaacaat taaagaatta attgaaaacc
     6421 cagaagactt attattagaa tcttaatcca caacacaaaa tagtttacta ttcctaaaaa
     6481 cgtaatcaaa
//
LOCUS       DAOBBH010000139         6454 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.139, whole genome shotgun sequence.
ACCESSION   DAOBBH010000139 DAOBBH010000000
VERSION     DAOBBH010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6454
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.139"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..497
                     /locus_tag="P6L01_002004"
     CDS             <1..497
                     /locus_tag="P6L01_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDP3031054.1"
                     /translation="YQNKPIMYNLDRIVVYMRLRRVGNKFSIKTWKFDHIKDPDRRKP
                     IDMDEKEWIDGGKFYQRPASIIAIYSAKYNGYKWMEMNGLGSFNTEILPKPKGARDVI
                     IQKGDLVKIDMQAKSVVINEEPMLSEKSFGSNYFNVDSGYSELIIQPENVFDTTVKWQ
                     DRYL"
     gene            513..4298
                     /locus_tag="P6L01_002005"
     CDS             513..4298
                     /locus_tag="P6L01_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031055.1"
                     /translation="MIHVLDFNDKIIDFLSTDDPSLVRAIHKRNVNDNSEMLELLISS
                     ERAEKFRERHRVIIRDSNKQWREFIINWVQDTMDGYTEIECIASYLADITTAKPYAPG
                     KFEKKTTSEALKDVLSDTGWEVSEQTEYDGLRTTSWTSYQTRYEVLKQLCTTYKMVLD
                     FYIELSSNTVKGRYVVLKKKNSLFKGKEIEYGKDLVGLTRKIDMSEIKTALIAVGPEN
                     DKGKRLELVVTDDEAQSQFNLPMRYIWGIYEPQSDDQNMNETRLSSLAKTELNKRKSA
                     VMSYEITSTDLEVTYPHEIISIGDTVRVKHRDFNPPLYVEAEVIAEEYNIISENSTYT
                     FGQPKEFKESELREEFNKRLNLIHQKLNDNISNINTIVKDVVDGELEYFERKIHKSDT
                     PPENPVNDTLWYDTSNPDVAVLRRYWNGRWIEATPNDVEKLGGITREKALFSELNNIF
                     INLSIQHASLLSEATELLNSEYLVDNDLKADLQASLDAVIDVYNQIKNNLESMTPETA
                     TIGRLVDTKTLFLEYRKKLQDVYTDVEDVKIAISDRFKLLQSQYTDEKYKEALEIIAT
                     KFGLTVNEDLQLVGEPNVVKSAIEAARESTKEQLRDYVKTSDYKTDKDGIVERLDTAE
                     AERTTLKGEIKDKVTLNEYRNGLEEQKQYTDDQLSDLSNNPEIKASIEQANQEAQEAL
                     KSYIDAQDNLKEKESQAYADGKISEEEQRAIQDAQAKLEEAKQNSELKARNAEKKANA
                     YTDNKVKESTDAQRRTLTRYGSQIIQNGKEIKLRTTKEEFNATNRTLSNILNEIVQNV
                     TDGTTIRYDDNGVAQALNVGPRGIRLNADKIDINGNREINLLIQNMRDKVDKTDIVNS
                     LNLSREGLDINVNRIGIKGGNNNRYVQIQNDSIELGGIVQRTWKGKRSTDDIFTRLKD
                     GHLRFRNNTAGGSLYMSHFGISTYIDGEGEDGGSSGTIQWWDKTYSDSGMNGITINSY
                     GGVVALTSDNNRVVLESYASSNIKSKQAPVYLYPNTDKVPGLNRFAFTLSNADNAYSS
                     DGYIMFGSDENYDYGAGIRFSKERNKGLVQIVNGRYATGGDTTIEAGYGKFNMLKRRD
                     GNRYIHIQSTDLLSVGSDDAGDRIASNSIYRRTYSATANLHITSAGTIGRSTSARKYK
                     LSIENQYNDRDEQLEHSKAILNLPIRTWFDKAESEILARELREDRKLSEDTYKLDRYV
                     GLIAEEVENLGLKEFVTYDDKGEIEGIAYDRLWIHLIPVIKEQQLRIKKLEESKNEG"
     gene            4288..4440
                     /locus_tag="P6L01_002006"
     CDS             4288..4440
                     /locus_tag="P6L01_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031056.1"
                     /translation="MKDNKQGLQANPEYTIHYLSQEIMRLTQENAMLKAYIQENKENQ
                     QCAEEE"
     gene            4487..4774
                     /locus_tag="P6L01_002007"
     CDS             4487..4774
                     /locus_tag="P6L01_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031057.1"
                     /translation="MAKEIINNTERFILVQIDKEGTERVVYQDFTGSFTTSDSASYAQ
                     DFKSEENAKKIAETLNLLYQLTGNQNGVKVVKEVVDRTDLSSDKSVDSETM"
     gene            4830..5204
                     /locus_tag="P6L01_002008"
     CDS             4830..5204
                     /locus_tag="P6L01_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031058.1"
                     /translation="MDDIQKIKKELSELVERVDDVEILANETADHVLELREEHKQHHN
                     ELRESHKELKDKQDKVVDENLEQTKILNRIEERYQTQVDVAQKNEEKTLAQNKWLVGA
                     IWALVTIVMIAVITASITALLP"
     gene            5644..6399
                     /gene="sep"
                     /locus_tag="P6L01_002009"
     CDS             5644..6399
                     /gene="sep"
                     /locus_tag="P6L01_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128623913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type P"
                     /protein_id="HDP3031059.1"
                     /translation="MLLSFIALTLITSPFVNCSEKSEEINGKDLQKKSELQGTALSNL
                     RQTYYHNGSAIIENKESNDQFLKNTILFNDFFTGHQWYNDLLVDLGSKDTANIYKGKK
                     VDLYGVYYGYQCTGGTPFKTACMYGGVTLHDNNQLEEEKKVPINLWIDGKQNTVPLGT
                     VKTNKKEVTVQELDLQSRHYLHETYNLYNTDAFNGKIQRGLIEFHPSSGDSVGYDLFG
                     AQGQYPDTQLRIYRDNKTIKSKNMHIDIYLYTT"
BASE COUNT         2476 a          880 c         1286 g         1812 t
ORIGIN      
        1 actatcagaa taaaccgata atgtataact tggacagaat cgttgtttat atgcggctca
       61 gaagagtagg taataaattt tctattaaaa cttggaaatt tgatcacatt aaagacccag
      121 atagacgtaa acctattgat atggatgaga aagagtggat agatggcggt aagttttatc
      181 agcgtccagc ttctatcata gctatctata gtgcgaagta taacggttat aagtggatgg
      241 agatgaatgg attaggttca ttcaatacgg agattctacc gaaaccgaaa ggcgcaaggg
      301 atgtcattat acaaaaaggt gatttagtga aaatagatat gcaagcaaaa agtgttgtca
      361 tcaatgagga accaatgttg agcgagaaat cgtttggaag taattatttc aatgttgatt
      421 ctgggtacag tgaattaatc atacaacctg aaaacgtctt tgatacgacg gttaaatggc
      481 aagatagata tttatagaaa ggagatgaga gtgtgataca tgttttagat tttaacgaca
      541 agattataga tttcctttct actgatgacc cttccttagt tagagcgatt cataaacgta
      601 atgttaatga caattcagaa atgcttgaac tgctcatatc atcagaaaga gctgaaaagt
      661 tccgtgaacg acatcgtgtt attataaggg attcaaacaa acaatggcgt gaatttatta
      721 ttaactgggt tcaagatacg atggacggct acacagagat agaatgtata gcgtcttatc
      781 ttgctgatat aacaacagct aaaccgtatg caccaggaaa atttgagaaa aagacaactt
      841 cagaagcatt gaaagatgtg ttgagcgata caggttggga agtttctgaa caaaccgaat
      901 acgatggctt acgtactacg tcatggactt cttatcaaac tagatatgaa gttttaaagc
      961 aattatgtac aacctataaa atggttttag atttttatat tgagcttagc tctaataccg
     1021 tcaaaggtag atatgtagta ctcaaaaaga aaaacagctt attcaaaggt aaagaaattg
     1081 aatatggtaa agatttagtc gggttaacta ggaagattga tatgtcagaa atcaaaacag
     1141 cattaattgc tgtgggacct gaaaatgaca aagggaagcg tttagagcta gttgtgacag
     1201 atgacgaagc gcaaagtcaa ttcaacctac ctatgcgcta tatttggggg atatatgaac
     1261 cacaatcaga tgatcaaaat atgaatgaaa cacgattaag ttctttagcc aaaacagagt
     1321 taaataaacg taagtcggca gttatgtcat atgagattac ttctactgat ttggaagtta
     1381 cgtatccgca cgagattata tcaattggcg atacagtcag agtaaaacat agagatttta
     1441 acccgccatt gtatgtagag gcagaagtta ttgctgaaga atataacata atttcagaaa
     1501 atagcacata tacattcggt caacctaaag agttcaaaga atcagaatta cgagaagagt
     1561 ttaacaagcg attaaaccta atacaccaaa aattaaacga caatattagc aatatcaata
     1621 ctatagtaaa agatgttgta gatggtgaat tagaatactt tgaacgcaaa attcataaaa
     1681 gtgatacacc gccagaaaat ccagtcaatg atacgctttg gtatgataca agtaaccctg
     1741 atgttgctgt cttgcgtaga tattggaatg gtcgatggat tgaagcaaca ccaaatgatg
     1801 ttgaaaaatt aggtggtata acaagagaga aagcgctatt cagtgaatta aacaatattt
     1861 ttattaattt atctatacaa cacgctagtc ttttgtcaga agctacagaa ttactgaata
     1921 gcgagtactt agtagataat gatttgaaag cggacttaca agcaagttta gacgctgtga
     1981 ttgatgttta taatcaaatt aaaaataatt tagaatctat gacacccgaa actgcaacga
     2041 ttggtcggtt ggtagataca aaaactttat ttcttgagta tagaaagaaa ttacaagatg
     2101 tttatacaga tgtagaagat gtcaaaatcg ccatttcaga tagatttaaa ttattacagt
     2161 cacaatacac tgatgaaaaa tataaagaag cgttggaaat aatagcaaca aaatttggtt
     2221 taacggtgaa tgaagatttg cagttagtcg gagaacctaa tgttgttaaa tcagctattg
     2281 aagcagctag agaatccaca aaagaacaat tacgtgacta tgtaaaaaca tcggactata
     2341 aaacagacaa agacggtatt gttgaacgtt tagatactgc tgaagctgag agaacgactt
     2401 taaaaggtga aatcaaagat aaagttacgt taaacgaata tcgaaacgga ttggaagaac
     2461 aaaaacaata tactgatgac cagttaagtg atttgtccaa taatcctgag attaaagcaa
     2521 gtattgaaca agcaaatcaa gaagcgcaag aagctttaaa atcatacatt gatgctcaag
     2581 ataatcttaa agagaaggaa tcgcaagcgt atgctgatgg taaaatttcg gaagaagagc
     2641 aacgcgctat acaagatgct caagctaaac ttgaagaggc aaaacaaaac tcagaactaa
     2701 aggctagaaa cgctgaaaag aaagctaatg cttatacaga caacaaggtc aaagaaagca
     2761 cagatgcaca gaggagaaca ctgactcgct atggttctca aattatacaa aatggtaagg
     2821 aaatcaaatt aagaactact aaagaagagt ttaatgcaac caatcgtaca ctttcaaata
     2881 tattaaacga gattgtccaa aacgttacag atggaacaac aatcagatat gatgataacg
     2941 gagtggctca agctttaaat gtggggccac gtggtattag attaaatgct gataaaattg
     3001 atattaacgg taatagagaa ataaaccttc ttatccaaaa tatgcgagat aaagtagata
     3061 aaaccgatat tgtcaacagc cttaatttat caagagaggg tcttgatatc aatgttaata
     3121 gaattggaat taaaggcggt aacaataaca gatatgttca aatacagaat gattctattg
     3181 aactaggtgg tattgtgcaa cgaacttgga aaggcaaacg atcaaccgat gatatattca
     3241 cacgtcttaa agatggacat ctaaggttta gaaataatac cgcaggcggt tcactttata
     3301 tgtcacattt tggtatttca acatatattg atggagaagg cgaagacgga ggttcatccg
     3361 gtactattca atggtgggat aaaacttaca gtgatagcgg tatgaatggc ataacaatca
     3421 attcctatgg tggtgtcgtt gcactaacgt cagataataa tcgggttgtt ctggagtctt
     3481 acgcttcatc gaatatcaaa agcaaacagg caccggtgta tttatatcca aacacagaca
     3541 aagtgcctgg attaaaccga tttgcattca cgctgtctaa tgcagataat gcttattcga
     3601 gtgacggtta tattatgttt ggttctgatg agaactatga ttacggtgcg ggtatcaggt
     3661 tttctaaaga aagaaataaa ggtcttgttc aaattgttaa tggacgatat gcaacaggtg
     3721 gagatacaac aatcgaagca gggtatggca aatttaatat gctgaaacga cgtgatggta
     3781 ataggtatat tcatatacag agtacagacc tactgtctgt aggttcagat gatgcaggag
     3841 ataggatagc ttctaactca atttatagac gtacttattc ggccacagct aatttgcata
     3901 ttacttctgc tggcacaatt gggcgttcga catcagcgcg taaatacaag ttatctatcg
     3961 aaaatcaata taacgataga gatgaacaac tggaacattc aaaagctatt cttaacttac
     4021 ctattagaac gtggtttgat aaagctgagt ctgaaatttt agctagagag ctgagagaag
     4081 atagaaaatt atcggaagac acctataaac ttgatagata cgtaggtttg attgctgaag
     4141 aggtggagaa tttaggatta aaagagtttg tcacgtatga tgacaaagga gaaattgaag
     4201 gtatagcgta tgatcgtcta tggattcatc ttatccctgt tatcaaagaa caacaactaa
     4261 gaatcaagaa attggaggag tcaaagaatg aaggataaca aacaaggatt acaagctaat
     4321 cctgaatata caattcatta tttatcacag gaaattatga ggttaacaca agaaaacgcg
     4381 atgttaaaag cgtatataca agaaaataaa gaaaatcaac aatgtgctga ggaagagtaa
     4441 tccttagcac tatttttata caaaaattta aggaggtcat ttaattatgg caaaagaaat
     4501 tatcaacaat acagaaaggt ttattttagt acaaatcgac aaagaaggta cagaacgtgt
     4561 agtatatcaa gatttcacag gaagttttac aacgtccgat tcagcaagtt atgcacaaga
     4621 ttttaaatct gaggaaaacg ctaaaaagat tgctgaaact ttaaatcttt tatatcaatt
     4681 aacaggcaat caaaacggtg tgaaagttgt gaaagaagtt gtggatagaa ctgacttgtc
     4741 atctgataaa tcagttgata gcgaaacaat gtaactatac taagttatga gcattacgct
     4801 catagctttc ttagaaagta ggtgtagttt tggatgatat tcagaaaata aaaaaagagc
     4861 tttctgaatt agttgaacgt gttgatgatg ttgaaatact agcaaatgaa acagctgatc
     4921 atgtgcttga acttagagag gaacataagc aacatcataa tgaactaaga gaatctcata
     4981 aagaacttaa agataagcaa gataaagttg tagatgagaa tttagagcaa acaaagatat
     5041 taaacagaat tgaagaaaga tatcaaacgc aagtagatgt tgcgcaaaaa aatgaagaaa
     5101 agacactcgc ccaaaataaa tggctcgtag gtgccatatg ggcgcttgta acaattgtta
     5161 tgattgcagt cattactgca tcaattactg cgttattacc ttaagggagg tggacataat
     5221 gagttgggca agatggttat catgttattt gtatggtcgt aaatgtaaat aatgtttttg
     5281 gtcagtgcat cggcactggc tttttatttt gattgaaaag aggtacgtac atggtattac
     5341 acagctcaaa agacaggaag catactgcaa gtgaagttgg gaagtgttgt caataccaag
     5401 taagtaagat atctgaaatg tataatagag taaaaatgaa atctttttat tatattatag
     5461 acaagtataa aaaaggtata gtaatatatg tatgtataag taaataatga tcattccata
     5521 attattgtat ataactaata attacttcaa caaaaataat ctattataca aatatttcgg
     5581 ataataacaa gtttatatgg aattatgctt tagaggtgag taaaataaaa aaaaacaaca
     5641 tttatactac tttcatttat tgccctaacg ttgataacaa gtccatttgt aaattgtagc
     5701 gagaaaagcg aagaaataaa tggaaaagat ttgcaaaaaa agtctgaatt gcagggaact
     5761 gctttaagca atcttagaca aacctattat cataatggaa gtgctataat tgaaaataaa
     5821 gagagtaatg atcaattttt aaagaacact atattgttta atgatttttt tacaggtcat
     5881 caatggtata atgatttatt agtagacctt ggttcaaaag acaccgccaa tatatacaaa
     5941 gggaaaaaag tggatttata tggtgtttat tatggttatc aatgtactgg gggtacacca
     6001 ttcaaaacag cttgtatgta tggcggtgta acgttacatg ataataatca attggaggaa
     6061 gaaaagaaag taccgattaa cttatggata gatggtaaac aaaatacagt acctttggga
     6121 acggttaaaa ctaataaaaa agaagtaact gttcaggagc tagaccttca gtcaagacat
     6181 tatttacatg aaacatataa tttatataac acagatgcat ttaatggaaa aatacaaaga
     6241 ggattaattg agtttcaccc ttcttctggt gattcggttg gttatgattt atttggtgct
     6301 caaggacaat atccagatac acagttgagg atatatagag ataataaaac gattaagtct
     6361 aaaaatatgc atattgatat atatttatat acaacttgag tgaaattttg gctgaaaaaa
     6421 tttaaaatct ataaaaagta ccataagtta tttt
//
LOCUS       DAOBBH010000140         6446 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.140, whole genome shotgun sequence.
ACCESSION   DAOBBH010000140 DAOBBH010000000
VERSION     DAOBBH010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.140"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            152..1711
                     /gene="rny"
                     /locus_tag="P6L01_002010"
     CDS             152..1711
                     /gene="rny"
                     /locus_tag="P6L01_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDP3031060.1"
                     /translation="MNLLSLLLILLGIILGVVGGYVVARNLLLQKQSQARQTAEDIVN
                     QAHKEADNIKKEKLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSD
                     LLDKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQ
                     RVEEELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPND
                     EMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDG
                     RIHPGRIEDMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQN
                     VLKHSIEVAHLASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKY
                     GENETVINAIHSHHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETL
                     SESYDGVEKAFAIQAGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTV
                     VRETRAVEYAK"
     gene            complement(2008..2223)
                     /locus_tag="P6L01_002011"
     CDS             complement(2008..2223)
                     /locus_tag="P6L01_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031061.1"
                     /translation="MSINIDPEKFAELVVMSNPSKFEDAEDIAKESLKLYINAYRLAE
                     RYSTIATNCYDTAEVIKELKKTDLQLK"
     gene            2397..3194
                     /locus_tag="P6L01_002012"
     CDS             2397..3194
                     /locus_tag="P6L01_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDP3031062.1"
                     /translation="MRIMFIGDIVGKIGRDAIETYIPQLKQKYKPTVTIVNAENAAHG
                     KGLTEKIYKQLLRNGVDFMTMGNHTYGQREIYDFIDEAKRLVRPANFPDEAPGIGMRF
                     IQINDIKLAVINLQGRAFMPDIDDPFKKADQLVKEAQEQTPFIFVDFHAETTSEKYAM
                     GWHLDGRASAVVGTHTHIQTADERILPKGTGYITDVGMTGFYDGILGINKTEVIERFI
                     TSLPQRHVVPNEGRSVLSGVVIDLDKEGKTKHIERILINDDHPFSTF"
     gene            3334..5094
                     /locus_tag="P6L01_002013"
     CDS             3334..5094
                     /locus_tag="P6L01_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HDP3031063.1"
                     /translation="MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSR
                     IKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVK
                     PEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGD
                     KVVEVNIQALNEGYQLMQSRLPEIDGDFELESTDALPHLYMIGNDAIGLGAIAAGSQF
                     MAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPG
                     LSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVA
                     PTDAEDAFYLTMEAFNLAEQYQCPVIVLSDLQLSLGKQTVEKLDYNRIEIKRGEIIQS
                     DIEREEDDKGYFKRYALTSNGVSPRPIPGVKGGIHHITGVEHNEEGKPSESASNRQQQ
                     MEKRMRKIEQLLIESPVEANLQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQ
                     IRQLHPFPTSVIQDAVNKAKKVVVVEHNYQGQLASIIKMNVNIHDKIENYTKYDGTPF
                     LPHEIEEKGKIIATEIKEMV"
     gene            5095..5961
                     /locus_tag="P6L01_002014"
     CDS             5095..5961
                     /locus_tag="P6L01_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HDP3031064.1"
                     /translation="MATFKDFRNNVKPNWCPGCGDFSVQAAIQKAAANIGLEPEEVAI
                     ITGIGCSGRLSGYINSYGVHSIHGRALPLAQGVKMANKDLTVIASGGDGDGYAIGMGH
                     TIHALRRNMNMTYIVMDNQIYGLTKGQTSPSSAVGFVTKTTPKGNIEKNVAPLELALS
                     SGATFVAQGFSSDIKGLTKLIEDAINHDGFSFVNVFSPCVTYNKINTYDWFKEHLTSV
                     DDIENYDSTDKQLATKTVIEHESLVTGIVYQDKETPSYESQIKELDDTPLAKRDIKIT
                     EDTFNALTEQFI"
     gene            6055..6348
                     /locus_tag="P6L01_002015"
     CDS             6055..6348
                     /locus_tag="P6L01_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HDP3031065.1"
                     /translation="MKDTLMSIQIIPKTPNNDNVIPYVDEAIKIIDESGLHFRVGPLE
                     TTVQGNMNECLILIQSLNERMVELECPSIISQVKFYHVPDGITIETLTEKYDE"
BASE COUNT         2377 a          923 c         1223 g         1923 t
ORIGIN      
        1 atagcattca atatagtgca aaagtgcaaa ttgataactt gacacttatc tcctataaac
       61 cgtacaatta atttgtatga tttatatata atttcataaa gtcatattga atttcatata
      121 aagagcaaac cctagaaaag gaggtgtttg tgtgaattta ttaagcctcc tactcatttt
      181 gctggggatc attctaggag ttgttggagg gtatgttgtt gcccgaaatt tgttgcttca
      241 aaagcaatca caagctagac aaactgccga agatattgta aatcaagcac ataaagaagc
      301 tgacaatatc aaaaaagaga aattacttga ggcaaaagaa gaaaaccaaa tcctaagaga
      361 acaaactgaa gcagaactac gagaaagacg tagcgaactt caaagacaag aaacccgact
      421 tcttcaaaaa gaagaaaact tagagcgcaa atctgatcta ttagataaaa aagatgagat
      481 tttagagcaa aaagaatcaa aaattgaaga aaaacaacaa caagtagatg caaaagagag
      541 tagtgttcaa acgttaataa tgaagcatga acaagaatta gaacgcatct ccggtctcac
      601 tcaagaagaa gctattaatg agcaacttca aagagtagag gaagaactgt cacaagatat
      661 tgcagtactt gttaaagaaa aagaaaaaga agctaaagaa aaagttgata aaacagcaaa
      721 agaattatta gctacagcag tacaaagatt agcagcagat cacacaagtg aatcaacggt
      781 atcagtagtt aacttaccta atgatgagat gaaaggtcga atcattggac gagaaggacg
      841 aaacatccgc acacttgaaa ctttaactgg cattgattta attattgatg acacaccaga
      901 agcggttata ttatctggtt ttgatccaat aagaagagaa attgctagaa cagcacttgt
      961 taacttagta tctgatggac gtattcatcc aggtagaatt gaagatatgg tcgaaaaagc
     1021 tagaaaagaa gtagacgata ttattagaga agcaggtgaa caagctacat ttgaagtgaa
     1081 cgcacataat atgcatcctg acttagtaaa aattgtaggg cgtttaaact atcgtacgag
     1141 ttacggtcaa aatgtactta aacattcaat tgaagttgcg catcttgcta gtatgttagc
     1201 tgctgagcta ggcgaagatg agacattagc gaaacgagct ggacttttac atgatgttgg
     1261 taaagcaatt gatcatgaag tagaaggtag tcatgttgaa atcggtgtag aattagcgaa
     1321 aaaatatggt gaaaatgaaa cagttattaa tgcaatccat tctcaccatg gtgatgttga
     1381 acctacatct attatatcta tccttgttgc tgctgcagat gcattgtctg cggctcgtcc
     1441 aggtgcaaga aaagaaacat tagagaatta tattcgtcga ttagaacgtt tagaaacgtt
     1501 atcagaaagt tatgatggtg tagaaaaagc atttgcgatt caggcaggta gagaaatccg
     1561 agtgattgta tctcctgaag aaattgatga tttaaaatct tatcgattgg ctagagatat
     1621 taaaaatcag attgaagatg aattacaata tcctggtcat atcaaggtga cagttgttcg
     1681 agagactaga gcagtagaat atgcgaaata atttttgtct ccctcacaaa ttagtgaggg
     1741 agctttttta agttgtagtc tcaatctaat tagacagcac tttatcggta ataactatag
     1801 taaacagtgg ttatttgaaa gtaagacgga ccttatatta aataagaagt tattgctttt
     1861 aataaaaatg ttttaggctt agtatttact atatttatat tatgtaaacc tataaagatg
     1921 agtgcttttc taaccaataa aaaagaagag aagatgtaac acatcttctc ttctgcaata
     1981 ttaattagga tttatttcta agttgagtta ttttaattgt aaatctgttt tctttaattc
     2041 ttttataact tctgcagtat cataacaatt tgttgcaatt gttgaatatc tctctgctaa
     2101 acgatatgca ttaatgtaaa gctttaaact ttctttagct atatcctctg catcttcgaa
     2161 ttttgatggg ttagacataa ccactaattc tgcaaatttt tctggatcaa tattaataga
     2221 catgtattta tttacaactc ctatttattt tgatgtctta atactaacat attgaagttt
     2281 tcagacaaag taatgtctct ctataattga agaaaaataa ttcaaagaat ttaatattta
     2341 ctttaaatat tagaacgtat gaattaaact agtatttatg agaggatgaa caaaacatga
     2401 gaataatgtt tataggggat atcgtaggta aaattggacg agacgcaatt gaaacgtaca
     2461 tacctcaact gaagcaaaag tataaaccaa cagttacaat tgtaaatgct gaaaatgcag
     2521 cacatggtaa aggtttgact gaaaaaatat ataaacaatt actaagaaat ggtgtagatt
     2581 tcatgactat gggtaatcac acatatggtc aacgtgaaat ttatgatttt atagatgaag
     2641 caaaacgact agtaagacca gcgaattttc cggatgaagc gccgggaatt ggtatgagat
     2701 ttatacaaat taatgatatt aaacttgcag ttattaatct gcaaggaaga gcgtttatgc
     2761 cagatattga tgatcctttt aaaaaggcag atcaattagt caaggaagca caagaacaaa
     2821 ctccgtttat atttgttgat tttcatgcag aaacaacttc tgaaaagtat gcaatgggat
     2881 ggcatttaga tggtagagct agcgctgttg ttggaacgca tacacacatt caaacagcag
     2941 atgaacgtat tttaccaaag gggacagggt atataacgga tgttggtatg acaggttttt
     3001 atgacggcat tttaggaata aataaaacag aggtaattga gcgttttatc actagtttgc
     3061 cacaaagaca tgttgttcca aatgaaggta gaagtgtatt atctggtgtt gttattgatt
     3121 tagacaaaga aggtaaaaca aagcacatcg aacgtatatt gataaatgat gaccatccat
     3181 tttcaacatt ttaaaattac gtaagtaaac attcgaattg gaccctatcg tccattagta
     3241 tgaatttaat atagtaccac tgtttacata gtaaatcggt ggttcttttt gttatcattt
     3301 aatatgaaat atatccatag gaggcatata actatgaaac cacaattatc gtggaaagtt
     3361 ggcggtcaac aaggcgaagg tattgaatca actggggaaa tcttcgctac ggctatgaat
     3421 agaaaaggat attatttata tggatataga catttttcaa gtcgtatcaa aggtggacat
     3481 acgaataata aaattagagt ttctacgacg cctgttcatg caattagtga tgatttagat
     3541 attttgattg catttgacca agaaacaatt gatgttaacc atcatgaaat gagagaagac
     3601 agtattattt tagctgatgc caaggctaaa cctgtgaaac cagaaggatg tcatgcacag
     3661 cttattgaat taccttttac agcaaccgct aaagaattag gtacagcatt aatgaaaaac
     3721 atggttgcaa taggtgctac tagcgcattg atgaatttga atacaaatac atttgaagaa
     3781 cttattacta atatgttttc taaaaaaggt gacaaggtag ttgaagtcaa tatccaagca
     3841 ttaaacgaag gttatcaatt aatgcaatcc cgcttacctg aaatcgacgg ggactttgaa
     3901 ttagagtcaa cagatgcact accacatcta tatatgattg gtaacgatgc cattggatta
     3961 ggtgcaattg ctgcaggttc acaatttatg gcggcatatc ctattacacc tgcgtctgaa
     4021 gttatggaat atatgattgc caatatatct aaagtaaacg gagcggttat tcaaacagaa
     4081 gatgaaattg ctgctgtaac tatggctatt ggtgcaaatt atggtggagt cagagcgttt
     4141 acggctagtg ctggtccagg tttatcttta atgatggaag caattggatt atctggtatg
     4201 actgaaacgc cattagtcat tattaatact caacgaggtg gaccttctac tggattacct
     4261 acgaaacaag aacagtcaga tttaatgcaa atgatttatg gtacacatgg tgatattcca
     4321 aaaatagttg tagcaccaac tgatgcagaa gatgcatttt atttaactat ggaagccttt
     4381 aatttagcag aacaatatca atgccctgtt atagttttaa gtgatttgca attatcttta
     4441 ggtaaacaaa ctgttgaaaa attagattat aatcgcattg aaattaaacg tggtgaaatc
     4501 attcaatctg atattgaacg tgaagaagat gataaaggtt atttcaagcg ttatgcgtta
     4561 acatccaatg gtgtttctcc tagacctatc cccggtgtta aaggaggtat tcatcatata
     4621 actggtgtgg aacacaatga agaaggtaaa cctagtgaat ctgcgtcaaa tagacaacaa
     4681 caaatggaaa aacgaatgcg taaaattgag cagttactaa ttgaatcgcc agtagaagct
     4741 aacttacaac atgaggatgc agatattctt tatatcggtt ttatttctac aaaaggtgca
     4801 attcaagaag gtagtaaccg tttgaatcaa caaggcataa aagttaacac tatacaaatt
     4861 agacaattgc atccattccc aacaagcgtt attcaagatg cagttaataa agcgaagaaa
     4921 gtcgttgtag tggagcacaa ttatcaagga caattggcta gtattataaa aatgaatgtc
     4981 aatattcatg ataagattga aaattataca aagtatgatg ggacaccttt cctaccacat
     5041 gaaatcgaag aaaaaggcaa aataattgct actgaaataa aggagatggt atagatggcg
     5101 acatttaaag attttagaaa taatgttaag cctaactggt gccccggatg tggcgatttc
     5161 tcagtacaag ctgcaattca aaaagcagcc gcaaatatag ggttagaacc tgaagaagta
     5221 gctatcatca ccggtatagg atgttctggc cgtctttcag gatatattaa ttcttatggc
     5281 gttcattcta ttcacggacg tgcattacct ttagctcaag gtgtaaaaat ggcgaataaa
     5341 gatttaactg ttattgcatc gggaggagat ggtgatggtt atgctatagg tatggggcat
     5401 acaatccatg ctttaagaag aaatatgaac atgacgtata tagtcatgga taatcaaatt
     5461 tatggtttga caaagggaca aacatcgccg tcatcagcag taggatttgt tactaaaaca
     5521 acgccaaaag gtaatataga aaaaaatgtt gcgcctttag aattagcatt atcatctggt
     5581 gccacatttg tagcccaagg tttttcaagc gatattaaag gattaacaaa actaattgaa
     5641 gatgcaatta atcatgatgg attttcattc gttaatgtct tttcaccatg tgtgacttat
     5701 aataaaatta acacatacga ttggtttaaa gaacatttaa caagtgttga tgacattgaa
     5761 aattatgatt ctacagataa acaattagcg actaaaactg ttattgaaca tgaatcttta
     5821 gtaactggta ttgtttatca agataaagaa acaccatcat atgaatctca aattaaagag
     5881 ttagatgata caccacttgc taaaagagat atcaaaatta ctgaagacac cttcaatgca
     5941 ttaactgaac aatttattta ataattaaaa ctgagtcgta taaatgtatt tataacagat
     6001 ccatttatgc tactcagttt tttactatta caaaaaataa aggagttttt aaaaatgaaa
     6061 gacacattaa tgagtataca aataattcct aaaacaccaa acaatgacaa tgttatacct
     6121 tacgtagacg aggcgattaa aataattgac gaatctggtt tgcattttag agtaggtccg
     6181 ttagaaacga cagtacaagg aaatatgaat gaatgtttaa ttttaataca atcattaaat
     6241 gaacgaatgg tggaacttga atgtccaagt attattagcc aagttaagtt ttatcatgtg
     6301 ccagatggca tcactattga aactttaact gaaaaatatg atgaataaca ttaaaagtga
     6361 agtaaactgg atttgaattg gcttgttaga gatgacgtat aactttaact gtttttgcac
     6421 tttatagtta aatttaatat aattat
//
LOCUS       DAOBBH010000141         6340 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.141, whole genome shotgun sequence.
ACCESSION   DAOBBH010000141 DAOBBH010000000
VERSION     DAOBBH010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6340
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.141"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(9..380)
                     /locus_tag="P6L01_002016"
     CDS             complement(9..380)
                     /locus_tag="P6L01_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDP3031066.1"
                     /translation="MLKKWLGMALITPILTFIIWVFNSHTIITYLNILFYVSLIIFIS
                     IFLILLVQEGIFDATSYGFRRLKYQMSSSKKKKSISDDPFFNPQEVKKEHYFVSTWII
                     PLLLINILYFIMTIVLSLILV"
     gene            623..1549
                     /locus_tag="P6L01_002017"
     CDS             623..1549
                     /locus_tag="P6L01_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3031067.1"
                     /translation="MGKYIFKRFIYMLISLFIIITITFFLMKLMPGSPFNDAKLNAEQ
                     KEILNEKYGLNDPVATQYLHYLKNVVTGDFGNSFQYHNQPVWDLIKPRLLPSFEMGLT
                     AMFIGVILGLILGVAAATKQNSWVDYTTTVISVIAVSVPSFVLAVLLQYVFAVKLRWF
                     PVAGWEGFSTAVLPSLALSAAVLATVARYIRAEMIEVLSSDYILLARAKGNSTMRVLF
                     GHALRNALIPIITIIVPMLASILTGTLTIENIFGVPGLGDQFVRSITTNDFSVIMAIT
                     LLFSTLFIVSIFIVDILYGVIDPRIRVQGGKK"
     gene            1549..2619
                     /locus_tag="P6L01_002018"
     CDS             1549..2619
                     /locus_tag="P6L01_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3031068.1"
                     /translation="MAENKNNLSINDDHSNAAMTHTSDAIASSDFIIRELDLNQEPEM
                     QRESKNFWQDAWAQLKRNKLAVVGMIGLIIIVIFAFIGPVINKHDYAEQNVEHRNLPA
                     KIPVLDKVPFLPFDGKDADGKDAYKAANAKENYWFGTDQLGRDLWTRTWKGAQISLFI
                     GVVAAMLDIFIGVVYGAISGFFGGRVDTIMQRILEVIASIPNLIVVILFVLIFEPSIW
                     TIILAMSITGWLGMSRVVRGEFLKLKNQEFVMASKTLGASKFKLIFKHILPNTLGAIV
                     VTSMFTVPSAIFFEAFLSFIGIGVPAPQTSLGSLVNDGRAMLLIYPHELFIPAMILSL
                     LILFFYLFSDGLRDAFDPKMRK"
     gene            2635..3717
                     /locus_tag="P6L01_002019"
     CDS             2635..3717
                     /locus_tag="P6L01_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3031069.1"
                     /translation="MTERILEVNDLHVSFDITAGEVQAVRGVDFYLNKGETLAIVGES
                     GSGKSVTTKAITKLFQGDTGRIKKGEILFLGEDLAKKPENELIKLRGKDISMIFQDPM
                     TSLNPTMQIGKQVMEPLIKHKNYSKAQAKKRALEILNLVGLPNAEKRFKAYPHQFSGG
                     QRQRIVIATALACEPKVLIADEPTTALDVTMQAQILDLMKELQQKIDTAIIFITHDLG
                     VVANIADRVAVMYGGQMVETGDVNEIFYDPKHPYTWGLLSSMPDLSTTNDTPLLAIPG
                     APPDLLHPPKGDAFARRSQYALDIDFKVEPPWFKVSPTHFVKSWLLDARAPKVELPEL
                     VKQRMKPMPNNYEKPLKVERVSFNEK"
     gene            3707..4648
                     /locus_tag="P6L01_002020"
     CDS             3707..4648
                     /locus_tag="P6L01_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDP3031070.1"
                     /translation="MKNDEVLLSIKNLKQYFNAGKKNEVRAIENISFDIYKGETLGLV
                     GESGCGKSTTGKSIIKLNDITSGEILYEGIDIQKIRKRKDLLKFNKKIQMIFQDPYAS
                     LNPRLKVMDIVAEGIDIHHLATDKRDRKKRVYDLLETVGLSKEHANRYPHEFSGGQRQ
                     RIGIARALAVEPEFIIADEPISALDVSIQAQLVNLLLKLQRERGITFLFIAHDLSMVK
                     YISDRIAVMHFGKIVEIGPAEEIYQNPLHDYTKSLLSAIPQPDPESERSRKRFSYIDD
                     EANNHLRQLHEIRPNHFVFSTEEEAAQLRENKLVTQN"
     gene            4667..6322
                     /locus_tag="P6L01_002021"
     CDS             4667..6322
                     /locus_tag="P6L01_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDP3031071.1"
                     /translation="MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLT
                     SLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDA
                     KWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALN
                     DETLQIELEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWK
                     QEDKTLLSKNQYYWDKKNVKLDKVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYK
                     DNKGLNFVLTSGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDT
                     LTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNEVTFSMNTEDTPDA
                     KISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLE
                     TMTTGSAQNNTDWGNKEYDQLLKVARTKLALQPNERYENLKKAEEMFLGDAPVAPIYQ
                     KGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK"
BASE COUNT         2292 a          914 c         1181 g         1953 t
ORIGIN      
        1 ctgtttcttt atactaagat gagcgacagc acaatcgtca taataaaata tagaatattt
       61 attaataata aggggattat ccatgtagaa acaaagtaat gctctttttt tacctcttgt
      121 gggttgaaaa atggatcatc agagatagac ttcttctttt tcgaagatga catttgatac
      181 tttaatcttc taaaaccata acttgtcgca tcaaaaatgc cttcttgtac aagtaaaatc
      241 aaaaatatgc taataaaaat aattaatgaa acataaaaca atatatttaa atatgtaatg
      301 atagtatggc tattaaaaac ccatataata aacgttaata ttggcgttat tagtgccatt
      361 ccaagccatt ttttcaacat ttgatcactc ccacttatag aaaactctta cgcatagttt
      421 acattaaaat gagacattga ggaatgattt tttaatttct gcagctttat tgaaattcta
      481 aaatcaatca ttcttcatta gtttaaagca aaaaaatatt gatatatagt aaatattgta
      541 tatataatat tagttaagat ttcagaaaat tttgaaggga atggaaattt agaaatcgga
      601 atttgttaga ggaggggatt agatggggaa atatattttc aaacgattta tttatatgct
      661 tatttcttta tttattatta ttacaattac atttttctta atgaaattaa tgccaggttc
      721 gccatttaac gatgctaaat taaatgctga acaaaaagaa attttaaatg aaaaatatgg
      781 attaaatgat cctgtagcta cgcagtattt acattattta aaaaatgttg ttacaggcga
      841 ttttggtaat tcattccagt atcataatca acctgtgtgg gatttgatta aaccgagact
      901 actaccttct tttgaaatgg gtcttacagc aatgttcatc ggtgtgatac tgggacttat
      961 tttaggtgtt gcagcagcta ctaaacaaaa ttcttgggtt gactatacaa ctacagttat
     1021 ttcagttatt gctgtatctg taccatcttt tgtacttgct gtacttttac aatatgtatt
     1081 tgcagttaaa ttaagatggt tcccagtagc tggatgggaa ggtttttcga ccgcggtatt
     1141 accgtcactt gcattatctg cagctgtttt agcaactgtc gccagataca taagagcaga
     1201 gatgatagag gtattaagtt cagactatat tttattagcg agagctaaag gtaattcgac
     1261 aatgcgtgta ctttttggac atgcacttag aaatgcttta attccaatta ttacaattat
     1321 cgttcccatg ttagcaagta ttttaacagg cactttaaca attgaaaata tttttggggt
     1381 tcctggatta ggggatcaat tcgtacgttc aattacaaca aatgatttct cagtaatcat
     1441 ggcaatcaca ctattattta gcacactgtt tatcgtttct atttttattg tagatatttt
     1501 gtacggtgtg atagatccac gaattcgtgt tcaaggaggt aaaaaataat ggctgaaaat
     1561 aaaaacaatt tgtcgattaa cgacgatcat tctaatgcag ctatgacgca tacctctgac
     1621 gctatcgcat catctgattt tattattaga gaattagact tgaatcagga acctgaaatg
     1681 caacgagaaa gcaaaaactt ttggcaagat gcttgggctc agttaaaacg aaataagtta
     1741 gctgttgtcg gtatgatagg tttaattatc attgtaatat ttgcttttat cggtccagtt
     1801 ataaataaac atgattatgc tgaacaaaat gtagaacata gaaatcttcc ggcaaaaata
     1861 cctgtattag acaaagttcc atttttacct tttgatggta aagatgcaga tggcaaggat
     1921 gcttataaag cagcaaatgc taaagaaaat tattggtttg gtactgatca gttgggtcga
     1981 gatttatgga caagaacatg gaaaggtgct caaatttcat tgtttatcgg tgttgttgca
     2041 gcgatgttag atatttttat tggcgttgta tatggtgcga tttctggatt cttcggtgga
     2101 cgtgtcgata cgattatgca acgtatactt gaagtcatag catctattcc gaatttaatt
     2161 gtcgtaattt tatttgtatt aatttttgaa ccatccattt ggacaattat attggctatg
     2221 tctatcacag gctggttagg catgagcaga gttgtacgtg gagaattttt aaaattgaaa
     2281 aatcaagagt ttgtcatggc ttcgaaaaca ttgggggctt caaaattcaa attgatattt
     2341 aagcatattt tacctaatac gttaggtgct atcgtggtta catcaatgtt tacagtacct
     2401 agtgctattt tcttcgaagc atttttaagt ttcattggta taggtgtacc tgcacctcaa
     2461 acatcgttag ggtcattagt aaatgatggg cgcgcaatgt tattaattta tccacatgaa
     2521 ttatttatac cagcaatgat tttaagttta ttaattctat tcttttactt atttagtgat
     2581 ggattacgtg atgcatttga tccgaaaatg cgtaaataaa agggggcata gcatatgact
     2641 gaaagaatat tagaagtaaa tgatttgcat gtttcctttg atattacagc aggggaagtg
     2701 caggcagtga gaggtgtaga tttttattta aacaaggggg aaacattggc aattgttggt
     2761 gaatcaggtt caggtaaatc tgtaacaaca aaagcaatta caaaattatt ccaaggggac
     2821 acaggaagaa ttaaaaaggg agaaatttta tttttagggg aagatttagc aaaaaaacct
     2881 gaaaatgagt taattaaatt acgtggcaaa gatatttcaa tgatctttca agatccaatg
     2941 acatctttaa acccaacgat gcaaattggt aaacaagtca tggaaccatt aattaagcac
     3001 aaaaattata gtaaagcaca agctaaaaag cgcgcattgg aaatactaaa tcttgtaggt
     3061 ttaccaaatg cagaaaaaag atttaaagca tatcctcatc aattttcagg tggacaaagg
     3121 caaagaattg ttattgcaac cgcattagct tgtgaaccta aagtgctcat tgctgatgaa
     3181 ccaacgactg cattagacgt aacgatgcag gcacaaattt tagatttaat gaaagaacta
     3241 caacaaaaaa tcgatacagc aattattttt ataacgcatg atttaggggt tgttgcgaat
     3301 attgctgata gagtggcagt tatgtatggt ggtcaaatgg ttgaaacagg agatgttaac
     3361 gaaatatttt atgatccaaa gcatccatat acatggggat tattatcgtc aatgcctgat
     3421 ttatcaacaa caaatgacac accattacta gcgattcctg gagcgccacc tgatttatta
     3481 cacccaccta aaggtgatgc atttgcgaga cgtagtcaat atgcattaga tattgatttt
     3541 aaagtagaac caccgtggtt taaagtttca ccgacacatt ttgtgaaatc ttggttatta
     3601 gacgcacgtg caccaaaagt tgaactaccc gagctggtaa aacaacgtat gaaaccgatg
     3661 cctaataatt atgaaaaacc actcaaggta gaaagggtgt cgttcaatga aaaatgatga
     3721 agtgctatta tctattaaaa atttaaagca atattttaac gcaggaaaga aaaacgaagt
     3781 gagagcgatt gaaaatattt cgtttgatat atacaaaggg gaaacattag gtttagtagg
     3841 agaatcgggg tgtggtaaat ctacaactgg taaatcaatt attaaactta atgatattac
     3901 aagtggagaa attttgtatg agggtattga tatacaaaag attcgtaaac gtaaagattt
     3961 gcttaaattt aataaaaaga tacagatgat ttttcaagac ccatatgcgt ctttaaatcc
     4021 taggttaaaa gtaatggata tagtagctga aggtattgat atccatcatt tagcaactga
     4081 taagcgtgac cgaaaaaaac gtgtctatga tttacttgaa actgttggat taagtaaaga
     4141 acatgccaat cgctatcctc atgaattttc aggtggacaa cgccaacgta ttggaattgc
     4201 ccgtgcatta gccgttgaac cagaattcat tatcgcggac gaaccaatat cggcattgga
     4261 tgtttcaatc caagctcaat tagttaattt attattaaaa ttacaacgtg aaagagggat
     4321 tacgttccta tttatagctc atgatctatc aatggtgaag tatatttcag atcgtattgc
     4381 agtcatgcat tttgggaaaa tagttgaaat tggaccggca gaagaaattt atcaaaatcc
     4441 attacacgat tatactaagt ctttattatc agccattcca caacctgatc ctgaatcaga
     4501 acgcagtcgc aaacgattta gttatattga tgatgaagca aataatcatt taagacaatt
     4561 acatgaaatt agaccgaatc actttgtctt tagtactgaa gaagaagcgg cacaactacg
     4621 agaaaataaa ttggtgacac aaaattaagg ggaaggggga aatgcaatga cgagaaaatt
     4681 tagaacactt attttaattt tgattgctac aattgcatta agtggttgtg ctaatgacga
     4741 tggtatttat tcagataaag gtcaagtatt cagaaaaatt ttgtcatcag acttaacatc
     4801 ccttgataca tcattaataa cggatgaaat atcttctgaa gtgactgcgc aaacattcga
     4861 aggtttatac acattaggaa aaggtgacaa accggtgtta ggtgttgcga aagcttttcc
     4921 tgaaaagagt aaagatggta aaactttaaa ggttaaatta agaagcgatg ctaaatggag
     4981 caatggtgac aaagtgactg cacaagactt tgtttatgct tggagaaaaa cagttgaccc
     5041 taaaacaggt tctgaatttg catacattat gggggacatt aaaaatgcga gtgatattag
     5101 tactggtaag aaacctgtag agcaattagg tatcaaagca ttaaatgatg aaacattaca
     5161 aattgaatta gaaaagccgg ttccatatat taatcaatta ttagcactta atacctttgc
     5221 acctcaaaat gaaaaagttg ccaaaaaata tggtaaaaat tacggtacgg cagctgatag
     5281 agcggtatac aatggtccat ttaaagttga tgattggaaa caagaagata aaaccttact
     5341 atctaaaaat cagtattatt gggataaaaa gaatgtaaaa ttagataaag tgaattataa
     5401 agttattaaa gacttacaag ccggtgcatc attgtatgat actgaatcag tagatgacgc
     5461 agttattact gcagatcaag taaataaata taaagacaac aaaggattaa actttgtgtt
     5521 aacgtctggg acattttttg taaaaatgaa tgaaaaacaa tatcctgatt ttaaaaacaa
     5581 aaatttaaga ttggctatcg cacaagcaat agataaaaaa ggatacgttg attcagtgaa
     5641 aaacaatggc tcaattcctt ccgatacact aacagccaaa ggaattgcga aagcgcctaa
     5701 tggcaaagat tatgcgagta ccatgaattc gcctttaaaa tataatccta aagaagcaag
     5761 agcacactgg gacaaagcta aaaaagagtt aggtaaaaat gaagtgacat tttcaatgaa
     5821 cacagaagat acaccagatg caaaaatatc tgctgaatat atcaaatcgc aagttgagaa
     5881 aaatttacca ggagttactt tgaaaattaa gcaattaccg tttaaacaaa gagtatcact
     5941 agaactgagt aacaattttg aagcatcact tagtggttgg tctgcagatt accctgatcc
     6001 tatggcttat ttagaaacaa tgaccacagg tagcgcacaa aataatacag actggggtaa
     6061 taaagaatat gatcaattac ttaaagtagc aagaaccaaa ttggcacttc aaccgaacga
     6121 acgatatgaa aacttgaaaa aagcagaaga aatgttccta ggagatgcac cggtagcacc
     6181 aatttatcaa aaaggtgttg cacatttaac aaatcctcaa gtaaaaggat taatttacca
     6241 taaatttggt ccaaataact cacttaaaca tgtatatatt gataaatcga tagataaaga
     6301 aacaggtaag aagaaaaaat aatatgcttt gtaaattagg
//
LOCUS       DAOBBH010000142         6295 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.142, whole genome shotgun sequence.
ACCESSION   DAOBBH010000142 DAOBBH010000000
VERSION     DAOBBH010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6295
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.142"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            239..1726
                     /locus_tag="P6L01_002022"
     CDS             239..1726
                     /locus_tag="P6L01_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031072.1"
                     /translation="MKTIQLHKTTLILIAILLFYTFMGILLPIMHDDLQWFSNYNTDI
                     LKVGFASLNGRYIGNIFEIIAVHVSWLRWLSYGLISMGIIWMIMHITRCKAWTSYYLL
                     AFSLMLILPSAIYADTYGWFAGFYNYATSTLISLFIIYYCINAIIYKEKQPVSVTVLF
                     YVLCFFGQLFMENVTLFHCIILILAFLYEFTANRTLNYKLLFSFMIATIGTIIMFSNP
                     NYRKILFEGSEYQQVSNNQGIFSKFAEMISTSLPYGVIFSQIIILSMIAALIIYLLLR
                     SDRYVHLTILKRRIIMIGFITLPLYYLLFYNQFLLNKNTDIGLVGFVNVIVCGYFAFS
                     IFVGIYLSINDRKTQITLYSLLIAICVSASTLVIVTPIEPGNFLIVYTIHVIILIILL
                     KEFRKYKSINVNFIKGASLVLAVIYLSAFIYVHYEHEKRVQLLKQQIKDHPRQEVYTV
                     ESLPFEHYMHHPSPINKNYQEWFNNYEELPKMTKVKYIPFGSDDS"
     gene            2020..4650
                     /gene="polA"
                     /locus_tag="P6L01_002023"
     CDS             2020..4650
                     /gene="polA"
                     /locus_tag="P6L01_002023"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDP3031073.1"
                     /translation="MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKI
                     LKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRY
                     ELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
                     PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHL
                     DEISGKKLKEKLQNSKEDALMSKELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKK
                     LEFKQLLSDIDQSASVEDAIEKTFEIETSFDNIDFTSLKEAAIHFELDGGNYLRNNIL
                     KFSLFTGEKHIVINADDINNYAELVSWLENPNTKKVVYDAKKTYVASHRLGIDIQNIS
                     FDIMLASYIIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVAS
                     ITDAIYFAKPNMDKQLEEYNQVELLADLELPLAKILSEMEEIGIFTDVHDLEEMEKEI
                     QEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVDVLEQLQ
                     GEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQ
                     NIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIH
                     TATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYGLSQSLGITRKKAKAFIDDYL
                     ASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQG
                     SAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENAL
                     QLDVPLKVDSSYGATWYDAK"
     gene            4666..5538
                     /gene="mutM"
                     /locus_tag="P6L01_002024"
     CDS             4666..5538
                     /gene="mutM"
                     /locus_tag="P6L01_002024"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDP3031074.1"
                     /translation="MPELPEVEHVKRGIEPYVINQKIEHVIFSDKVIEGKAQGKETII
                     KGIELDTFKTLSEGYTITNVERRSKYIVFQLDNKREQRTLISHLGMAGGFFIVDELED
                     IMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFLEIAPEPFTNEAL
                     TYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKVKDLTHQQQ
                     EMVFYYVREVLEEGIKHGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKI
                     IATRNSHYCPVCQK"
     gene            5554..6177
                     /locus_tag="P6L01_002025"
     CDS             5554..6177
                     /locus_tag="P6L01_002025"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDP3031075.1"
                     /translation="MPKVIGLTGGIASGKSTVSELLSVFGFKVVDADKAAREAVKKGS
                     KGLAQVREVFGDEAIDENGEMNRRYMGDLVFNHPEKRLELNAIIHPIVRDIMEEEKQE
                     YLKQGYNVIMDIPLLFENELENTVDEVWVVYTSESIQMDRLMQRNNLSLEDAKARVYS
                     QISIDKKSRMADHVIDNLGDKLELKQNLERLLEEEGYIEKPNYGEED"
BASE COUNT         2296 a          818 c         1127 g         2054 t
ORIGIN      
        1 tatttgaacg gaaataaatc ttaatttgaa cacttgaata cacaagtgta atcattcgtc
       61 atagaaaaag acgaggggcg atttagataa attgttgaaa gtaatattaa ataccttgtt
      121 tatctaattt gattgaggaa acatgcatag gtgaaaatta gaaatacatt aaaaatattg
      181 aattaatttt tcgacctaca agttgaggaa aatttaacta gatttgagga ttagcaaagt
      241 gaaaacaatt caattacaca agacaacttt gatattaatt gcgatattat tgttttatac
      301 ttttatgggt atcttactac caatcatgca cgatgattta caatggttta gcaattataa
      361 tactgatatt ttaaaagtag gatttgcatc acttaatggt cgctatatcg gaaatatctt
      421 tgaaattata gctgtacatg taagctggtt acgttggctt tcatatggtc tcattagtat
      481 gggcatcatt tggatgatta tgcacattac acgttgtaaa gcgtggacaa gctattattt
      541 gttagcattt tctttaatgt taattttacc aagtgctatt tatgcagata catatggctg
      601 gtttgcagga ttttataatt atgcgacatc aacactaatt tcactcttta ttatttatta
      661 ttgtattaat gcaattatat ataaagaaaa gcagccggta agtgttactg tactattcta
      721 tgttttatgc ttcttcggtc aattatttat ggagaatgtg acattattcc actgtatcat
      781 tttaatttta gcttttctgt atgaatttac tgcaaatcga acacttaatt ataaattatt
      841 gttttcattt atgattgcta ctataggtac aatcattatg ttttccaatc caaattatcg
      901 caaaatttta tttgaaggat cagaatatca acaagtttca aataatcaag gtatcttttc
      961 aaaatttgca gaaatgatat caacgtcact tccatatggt gtcattttca gtcagattat
     1021 aattttaagt atgattgcag cgcttattat ttatctactg cttagaagtg atcggtatgt
     1081 acatttaaca atattaaagc gtcgtatcat tatgatagga tttatcactt taccgcttta
     1141 ttatttacta ttttacaatc agtttttatt aaataaaaat acagatattg gattggttgg
     1201 ttttgtgaac gtaattgtat gtggttactt tgcgttttcg atatttgtag gtatttattt
     1261 gtctattaat gatcgtaaaa cacaaatcac tttatattcc ttacttattg caatatgtgt
     1321 ttcggcgtcg acactagtca ttgttactcc aatagaacca ggtaattttt tgattgttta
     1381 tacgatacat gtaatcattt taattatttt attaaaagaa tttcggaaat ataagtcaat
     1441 caatgttaat tttattaaag gtgcatcact agttttagct gtgatatatc ttagtgcatt
     1501 tatttatgtt cattatgaac atgaaaaaag agttcagtta ttaaaacaac aaattaaaga
     1561 tcatccacga caagaggttt atacagtgga atcattgccg tttgaacatt atatgcatca
     1621 tccgtcacca atcaataaaa attaccaaga gtggtttaat aattatgaag aattaccgaa
     1681 aatgaccaaa gttaaatata ttccatttgg ttcagacgat tcataactta ttcttaccta
     1741 taaaaattta aaatagcagc ataaaaatat taaaaagtag acataatcat gaaatgataa
     1801 tgtgcatctc aaaagttgga cttttaacct gactttatgg agtgctcatc aagaacaatc
     1861 atgattatgt cttttttatt ttttagctaa gtctatatag gcacttgttt tatttgaaat
     1921 taggtcgtgt ataaatactc tatgagatag tttctttaat tacgacatca cagtcgaata
     1981 tgctaaaata tatataaatt agttctggag gttaaaattg tgaataaatt agtattaatc
     2041 gatggtaata gcttaagttt tagagctttt tatgcattac ctttgttatc aaacaaagca
     2101 ggcattcata ccaatgcagt atatggtttt gcaatgttac tagagaagat tttaaaagaa
     2161 gaaaagccaa atcatttttt agtagcgttt gatgcaggta aaacaacttt cagacatgaa
     2221 aaatatagtg aatataaagg tggacgccag aaaacgccgc ctgaactaag tgagcaattt
     2281 ccttatatac gccaattatt agatgcttat catattaaac gttatgaatt agataactat
     2341 gaggcagatg atattatcgg aactttaagt aaagaagcag ataaagcggg ctttcagaca
     2401 attattataa cgggagaccg agatttaaca caacttgcaa cggataatgt aaccatttat
     2461 tacactaaaa aaggtgttac tgatgttgat cattatacac ctgactttat tgcggaaaaa
     2521 tacaacgggt taacacctaa tcaaattatt gatatgaaag gattaatggg agatacttct
     2581 gataatatac caggtgttgc aggtgttgga gagaaaacag caataaaatt gctaaaccaa
     2641 tttgacacag tagaaggtgt ctatgaacat ttagatgaaa tttcaggtaa aaagttaaaa
     2701 gaaaaacttc aaaatagcaa agaagatgca ttaatgagta aagaattagc aacgattaat
     2761 gtagatagtc cgattgaagt aaaacttgaa gatacgttaa tgactcatca agatgaacaa
     2821 caagaaaaaa tcgaattgtt taagaagtta gaattcaaac aattgttgtc tgacattgat
     2881 caatcagcaa gcgttgaaga tgcaatagaa aagacatttg aaattgaaac gtcttttgat
     2941 aatattgatt ttacttcatt gaaagaagcg gccatccatt tcgaattaga cggtggtaac
     3001 tatttgcgaa ataatatttt aaaattctct ttatttacag gtgagaaaca tattgtaatt
     3061 aatgcggatg acataaataa ttatgccgaa ctagtttcat ggttagaaaa tccgaatacg
     3121 aaaaaagtcg tatatgatgc taaaaaaaca tatgtagcat cacatagatt gggaattgat
     3181 attcaaaata tttctttcga tattatgttg gccagttata taattgatcc atctcgtacg
     3241 attagtgatg ttcaatcagt tgtttcattg tacggtcaaa gttttgtgaa agacgatgta
     3301 agtatatatg gaaaaggtaa gaaatttaag gtacctgaag atgatgtttt aaatccatat
     3361 gttgcttcta ttactgatgc aatttacttt gcgaaaccaa atatggataa acaattagaa
     3421 gaatacaatc aggtagaact cttagctgat ttagagctac cgctagctaa gattttaagt
     3481 gaaatggaag aaattggtat atttacagat gttcatgatt tagaagaaat ggaaaaagaa
     3541 attcaagaaa aattagacgt cttgattcga aatatccatg atgcagctgg tgaagatttt
     3601 aatataaatt ctcctaaaca attaggtgtt gtattgtttg agacattaca attacctgtt
     3661 attaaaaaga cgaaaacagg atattctact gctgtagatg tcttagagca attgcaaggt
     3721 gaacatccta tcattgatta tattttagaa tatcgtcaat tatctaagtt acagtctact
     3781 tatgttgaag gattgcaaaa ggtaattagt gatgatcaac gtatccatac acgttttaac
     3841 caaactttag cgcaaactgg acgtttatca agtgtagatc ctaatttaca aaatattccg
     3901 gttagacttg aagaagggcg taaaataaga aaagccttta aaccaacttc aaaagatagc
     3961 gttatattat cagcagatta ttctcaaatt gaattgcgtg tattagcaca cattacacaa
     4021 gatgagagta tgaaagaagc atttatcaac ggcgatgata ttcatacagc aactgctatg
     4081 aaagtatttg gtgtagaggc tgatcaagtc gatagtttaa tgcgtcgtca agcaaaagcg
     4141 gttaactttg gaattgttta tgggataagt gattatggtt taagtcaaag tttaggtatt
     4201 actcgtaaaa aagcaaaagc attcattgat gattatttag ctagtttccc aggtgtgaaa
     4261 caatatatgt ctgatattgt aaaagatgcc aaagctttag gttacgtgga aacattgcta
     4321 catcgtcgac gctatattcc tgatattacg agtcgtaact ttaatttacg cggctttgct
     4381 gaacgtactg ctatgaatac gccaatacaa ggcagtgctg cagatatcat taaactggca
     4441 atggttaaat ttgcacaaaa aatgaaagag acaacatatc aagctaaact attattacaa
     4501 gtacacgatg aattaatttt tgaagtacct aagtcagaag tagattcatt tagtgaattt
     4561 gtagaagaga taatggaaaa tgcattgcaa ttagatgttc cattaaaagt agactcaagt
     4621 tatggtgcaa cttggtatga tgcaaaataa agtgaggtta atggtatgcc cgaattacca
     4681 gaagtagaac atgtaaaaag aggaattgaa ccctacgtta taaatcaaaa aattgaacac
     4741 gttatatttt cggataaagt aatcgaaggt aaagctcaag gcaaggaaac aattataaaa
     4801 ggaattgaat tagatacttt taaaacttta tcggaaggct ataccattac aaatgtagaa
     4861 cgaagaagta aatatatcgt ttttcagcta gataataaac gagaacaacg tacattaatt
     4921 agtcatttag gtatggcggg tggttttttc attgtagatg aacttgaaga tattatgata
     4981 cctaattatc gtaagcattg gcatgttatt tttgagttgt caaatgacaa aaagttaatt
     5041 tattcagata tacgtcgttt tggagaaatt agaaatgttg cgtctgtagc atcttatcct
     5101 tcatttttag aaatagcgcc tgaacctttt acaaatgaag cattgacgta ttatttaaat
     5161 cgaattcatc aacagtctaa taagaataaa ccaatcaaac aagtgatttt ggatcataaa
     5221 gtaatcgcag gttgcggaaa tatttacgca tgtgaagcgt tgtttcgtgc aggtgtttta
     5281 ccagataaaa aagtaaaaga cttaacacac caacaacaag aaatggtttt ttattatgtt
     5341 agagaagtac tagaagaagg tattaagcat ggcggtacaa gtatttcaga ttatcgtcat
     5401 gctgatggaa aaactggtga gatgcaattg catcttaatg tatataagca gccggtatgc
     5461 aaagtgtgtg gaagtcaaat tgaaacaaaa atcatcgcga caagaaatag tcattattgt
     5521 ccagtatgtc aaaaataaag aaagagtgaa attatgccga aagttattgg tctaacaggt
     5581 ggaatcgcct caggaaaatc aacagtatca gaactcttat ccgtattcgg ttttaaagta
     5641 gtagatgctg ataaagcagc cagggaagct gttaaaaagg ggagtaaagg tttagctcaa
     5701 gtacgagaag tctttggtga tgaagcaatt gatgaaaatg gtgagatgaa tcgtcgttat
     5761 atgggtgatt tagtgtttaa ccatccagaa aaacgcttag aattaaatgc tatcatacat
     5821 cctatcgtgc gagatattat ggaagaagaa aagcaagaat atttaaaaca aggatataat
     5881 gtaatcatgg atattccatt attatttgaa aatgaattgg aaaatacagt agacgaagtg
     5941 tgggttgtat acacttctga aagtatacaa atggatcgtt taatgcaacg taataatctg
     6001 tcattagaag atgcgaaagc acgtgtctat agccaaattt ctattgataa aaaaagccga
     6061 atggccgatc atgttatcga taatttaggg gataaacttg aattaaaaca aaaccttgag
     6121 agattgttag aagaagaagg ttatattgaa aagccgaatt acggagaaga agattaatat
     6181 tacactataa ataagtcatt actttacgta cgcgttgatg tatgtaagta atgactattt
     6241 tttataaaaa agataaataa atcaacggaa aacgctttca aaattcatat aatat
//
LOCUS       DAOBBH010000143         6256 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.143, whole genome shotgun sequence.
ACCESSION   DAOBBH010000143 DAOBBH010000000
VERSION     DAOBBH010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.143"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            271..1563
                     /gene="obgE"
                     /locus_tag="P6L01_002026"
     CDS             271..1563
                     /gene="obgE"
                     /locus_tag="P6L01_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDP3031076.1"
                     /translation="MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASV
                     VFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDE
                     VLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLAD
                     VGLVGFPSVGKSTLLSIVSKAKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIE
                     GASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRP
                     QIVVANKMDLPESQDNLNLFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDV
                     DFTVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAV
                     RRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE"
     gene            1576..2031
                     /locus_tag="P6L01_002027"
     CDS             1576..2031
                     /locus_tag="P6L01_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDP3031077.1"
                     /translation="MDNKDYKKFYLIREDVLPESVVKTLKIKDALKSDPTLSIYDAVK
                     QFDLSRSAFYKYRETIFPVDDKMLDHREFTLILYVTDIVGMLARVLDVISKLELSVLT
                     IHQSIPMEEKATITLSLNAKSKETSVEDVIGALRNLDYVSKVELISMSM"
     gene            2045..2647
                     /gene="ruvA"
                     /locus_tag="P6L01_002028"
     CDS             2045..2647
                     /gene="ruvA"
                     /locus_tag="P6L01_002028"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDP3031078.1"
                     /translation="MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEV
                     LIRTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGIGPKSALAILATSTPNEVKRAIE
                     NENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTVQDQFVQEAML
                     ALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS"
     gene            2679..3683
                     /gene="ruvB"
                     /locus_tag="P6L01_002029"
     CDS             2679..3683
                     /gene="ruvB"
                     /locus_tag="P6L01_002029"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDP3031079.1"
                     /translation="MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIK
                     AAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVNIRTVSGPSLERPGDLAAILSGL
                     QPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
                     RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTP
                     RVANRLLKRVRDFQQVNEDEQIYIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNG
                     GPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATPLAYEHFAKSNEERG
                     "
     gene            3685..4710
                     /gene="queA"
                     /locus_tag="P6L01_002030"
     CDS             3685..4710
                     /gene="queA"
                     /locus_tag="P6L01_002030"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDP3031080.1"
                     /translation="MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFK
                     DIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRI
                     KVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
                     PDRYQTVYAKESGSAAAPTAGLHFTDELLTEIKNKGVNIAFVTLHVGLGTFRPVSVDD
                     VNDHEMHSEYYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGW
                     TNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSTRENVLNAYKTAVNLEYRFFSF
                     GDAMLII"
     gene            4733..5872
                     /gene="tgt"
                     /locus_tag="P6L01_002031"
     CDS             4733..5872
                     /gene="tgt"
                     /locus_tag="P6L01_002031"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016425271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDP3031081.1"
                     /translation="MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVK
                     TMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFS
                     LSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYV
                     KKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGG
                     LSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIA
                     RNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLT
                     TIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF"
     gene            5891..6151
                     /gene="yajC"
                     /locus_tag="P6L01_002032"
     CDS             5891..6151
                     /gene="yajC"
                     /locus_tag="P6L01_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDP3031082.1"
                     /translation="MQFSLLIYIVVIFAVMYFLMIRPQQKRAKQHRELINNIQSGQRI
                     TTIGGIKGTVKAVDETTVVITVNGHGTELTFEKPAIKQVDPS"
BASE COUNT         2207 a          861 c         1296 g         1892 t
ORIGIN      
        1 tattaaaaaa tgggaatttt agacgttata ctaaatgtac aactgcataa gagcccctaa
       61 tcgctaaact ataaggggac aaaagaatac agttgcagta taaaaagcaa aaaaagtatc
      121 atatttggta ttcggaaata tcagcaattt gagtaaagta aatttaaaag caactgcata
      181 agagccccta attatttaca atatataagg ggctctaata tgctataatt attgggaaaa
      241 tgaaaattat atgtaaaaga ggtgagatat atgtttgtcg atcaagtcaa aatatctctt
      301 aaagccggtg atggtggtaa tggtattacc gcatatagaa gagaaaaata tgtaccattt
      361 ggtggaccag ctggcggtga cggtggtaaa ggtgcttcag tcgtatttga agtggatgaa
      421 ggtttaagaa cgttattaga ttttagatat caacgtcatt ttaaagcaag caaaggtgaa
      481 aatggccaaa gtagtaatat gcatggtaaa aatgcggaag atttagtatt aaaagttcca
      541 cctggtacaa ttattaaaaa tgttgaaaca gacgaagtgt tagcagatct tgttgaagat
      601 ggtcaaagag ctgtagtagc gaagggcggt cgaggtggcc gaggtaattc acgttttgca
      661 acacctagaa accctgcacc tgacttcagt gaaaaaggtg aaccaggtga ggaattagat
      721 gtatctttag aattgaaatt attagctgat gtaggattag taggtttccc tagtgtgggt
      781 aaatcgactt tattatctat cgtttcaaaa gctaagccta aaattggggc atatcatttt
      841 acaacgatta aaccaaatct aggtgttgtt tcaacgcctg atcaacgtag ttttgttatg
      901 gcagatttac caggtttaat tgaaggtgca tctgatggcg ttggattagg acatcaattt
      961 ttaagacatg tagagagaac aaaagttatt gttcacatga ttgatatgag cggttctgaa
     1021 ggtagagaac ctattgaaga ttataaagtc attaatcaag aattagctgc gtacgagcaa
     1081 cgtttagaag atagacctca aatcgtagta gctaacaaga tggatttacc tgaatcacaa
     1141 gataatttaa acttgtttaa agaagaaatt ggcgaagatg tgccagttat tccagtttca
     1201 acaataacgc gtgataatat tgatcaatta ttatatgcaa tagcagataa attagaagaa
     1261 tataaagatg ttgacttcac agttgaagaa gaggagtcag ttggcattaa ccgagtatta
     1321 tataaacata caccgtcaca agataaattt acaatttcaa gagatgatga tggtgcttat
     1381 gtggtaagtg gtaatgctat tgaaagaatg tttaaaatga ctgactttaa cagtgatcca
     1441 gcagtacgtc gatttgctcg tcaaatgcgt tcgatgggta ttgatgatgc acttagagaa
     1501 cgtggttgta aaaatggtga tatcgttaga attcttggcg gagaatttga attcgttgaa
     1561 taggagcgta acatgatgga caataaagat tataaaaagt tttatttaat tagagaagat
     1621 gtcttgcctg aatccgtggt taaaacattg aagattaaag atgccttaaa aagtgatccg
     1681 acattgtcca tttatgatgc cgttaaacag tttgatctat ctagaagtgc tttttataaa
     1741 tatagagaaa cgatatttcc agtagacgat aaaatgcttg accatcgaga atttacatta
     1801 attttatatg taactgatat tgtgggtatg ttggcacgtg tactagatgt tatatcaaag
     1861 ttagaactat ctgtattaac gattcatcaa agtattccaa tggaagaaaa agcaacaata
     1921 acattatcac tgaatgctaa atctaaagaa acttcagtag aagatgttat tggtgctttg
     1981 agaaatttag attatgtatc aaaagtagaa ttaattagta tgagtatgta aggatgtgcc
     2041 tataatgtac gcgtatgtca aaggtaagtt aacacattta tatcctacac acgtagttgt
     2101 tgaaactgct ggtgttggtt atgaaattca aacaccaaat tcttatcgtt ttcaaaagca
     2161 tctagatcat gaagttttaa ttcgtacatc tttaattgtt cgtgaagatg cacaattatt
     2221 gtatggattt agtagtgaag aagagaaaga tatgttcttg agtttaatta aagttactgg
     2281 tattggtccg aaatcagctt tagctatttt agcgacaagt acgcctaatg aagtaaaacg
     2341 tgccattgaa aatgaaaatg atacgtattt aactaaattc ccaggaattg gtaagaaaac
     2401 ggcaagacag attgtcttag atttaaaagg taaagtgaaa atcactgaag aagatagcga
     2461 ttcattatta caagtagacg ctacttcgac ggtgcaagat caattcgtgc aagaagcaat
     2521 gttagcgtta gaagcattag gttattctaa acgagagctt gcaaaagttg agaaaacgtt
     2581 aaataaaaat aaatatgact cagttgatga agctgttaag gcaggtcttc aattagttgt
     2641 atcttaattt taaatagatt aataggggaa gtgttgtcat gaatgagcgt atggttgatc
     2701 aatcaatgca tagtgaagaa actgatttcg aattgtcgct tagacctacg agattacgac
     2761 aatatattgg tcaaaattca ataaaaagta atttagaagt atttattaaa gcggctaaac
     2821 ttcgtcatga accattagat catgtattgc tttttggccc ccctggatta ggtaagacaa
     2881 cattatctaa tatcattgcc aatgaaatgg aagttaatat acgtacagta tcagggcctt
     2941 cattagaaag acctggtgat ttggctgcaa ttttatcagg acttcaacct ggagatgtat
     3001 tgtttattga tgaaatacac agactgagta gtgttgttga agaagtgtta taccctgcaa
     3061 tggaagattt ctttttagat attatcattg gtaaaggcga tgaggctaga agtatccgta
     3121 tcgacttacc tccattcact ttggtaggtg caacaacgcg agctggcagc ttaacaggtc
     3181 cactaaggga tcgatttggt gtgcacttaa gattagaata ttataatgaa tcagatttaa
     3241 aagaaatcat tattagaaca gctgaggttt taggcacagg tattgatgaa gaaagtgcca
     3301 ttgaacttgc taaacgttct agagggactc caagagtagc aaatcgacta ttgaagcggg
     3361 taagagactt ccagcaagtg aatgaagatg aacaaatata cattgaaaca acgaagcacg
     3421 cattaggttt acttcaagtt gatcaacacg gactagatta cattgatcat aaaatgatga
     3481 actgtattat taagcagtat aatggtggac ctgttggttt agatacgatt gccgtaacaa
     3541 ttggtgaaga acgtattaca attgaggacg tttatgagcc atttcttatt cagaaaggct
     3601 ttttagaacg tacgccacgt ggcagaaaag caacaccatt agcttatgaa cattttgcaa
     3661 agtcgaatga ggagagagga taacgtgaat attgaagaat ttgactatga cttaccagaa
     3721 tcattaattg ctcaaacgcc tttaaaagat cgtgatcata gtcgtttatt agtcatggat
     3781 agagaaactg gtgaaatgaa acatttacat ttcaaagata tcattgagta ttttagacct
     3841 ggtgatacat tagtgcttaa cgatacgcga gtaatgccag ctagactttt tggtttaaaa
     3901 gaagaaactg gtgcaaaagt tgaaatgtta atgttaactc aaattgaagg taatgattgg
     3961 gaagtcttac tgaaaccagc taagcgtatt aaagttggta ataaattgaa ttttggtaat
     4021 ggcaaaatta tagctgagtg cataaaagaa atggatcaag gtggacgcat catgcgttta
     4081 cattatgaag gtattttaca agaaagatta gatgaattag gggaaatgcc actgccacca
     4141 tacatcaaag aacgtttaga tgatccagat cgttatcaaa cagtttacgc taaagaaagt
     4201 ggttcagcgg cagcaccaac agcaggatta cattttactg atgagttatt aactgaaatt
     4261 aaaaataaag gcgttaatat cgcatttgtt acattacatg ttgggttagg tacgtttaga
     4321 ccggtgagcg tcgatgatgt gaatgaccac gaaatgcata gtgaatatta tcaaatgaca
     4381 caagaaacag ctgatttatt aaatgatact aagtccaaag gacatcgcat tatatcagtt
     4441 ggtacaactt caacacgtac acttgaaaca attcgacgcg atcatgataa atttgttgaa
     4501 acgagtggct ggactaatat atttatttat ccaggatttg attttaaagc aattgatggc
     4561 cagattacta attttcattt accaaaatca acattagtta tgctagtatc agcgtttagt
     4621 actcgtgaaa atgttctgaa tgcttataaa acagcagtaa atttagaata tagattcttt
     4681 agttttggcg atgcaatgtt aattatataa aaagaatgtg aggattttga atatgcctgc
     4741 agtaacatac gaacacatta aaacttgtaa acaatcaggt gcgcgtttag gtatcgtgca
     4801 cacaccacac ggttcatttg aaacacctat gtttatgcca gttggtacta aagcaaccgt
     4861 taaaacaatg agtccagaag agttaagaca aattgaagca aaaatcattt tgggcaacac
     4921 atatcatttg tggttacaac ccggaaatga tattatcaaa cacgctgggg gattacataa
     4981 attcatgaat tgggatggtc cgattcttac agattcaggc ggtttccaag tgtttagttt
     5041 aagtaattta cgtaaaatta cagaagaagg cgtggaattt agacatcata ctaatgggtc
     5101 taaattattt ttgagtcctg agaaatcaat gcaaattcaa aatgatttag gatctgatat
     5161 tatgatggca tttgatgaat gtccaccgat gcctgctgaa tatgattatg taaaaaaatc
     5221 tattgaacgt acaacacgtt gggcgaaaag atgtctagat gcacaccaaa gacctgaaga
     5281 tcaagcattg ttcggcatta tacaaggtgg cgaatatgaa gatttaagag aacaaagtgc
     5341 aaaggattta gtagaattag attttcctgg ttatgcaatc ggcggtttgt cagttggtga
     5401 acctaaaccg gttatgtata aaatggttga acatacagag cagtttatgc ctaaagataa
     5461 accaagatat ttaatgggtg taggatctcc agatgcgtta atcgaatgta gtattcgcgg
     5521 catggatatg tttgattgtg tcttaccgac acgtattgcc agaaatggta cttgtatgac
     5581 atcgcaaggt cgtttagtta ttaaaaatgc aaaatttgca gatgatttaa gaccgttaga
     5641 tgagaattgt gactgttata catgtcaaaa ctattcaaga gcgtatatac gtcatttaat
     5701 caaggcagag gaaacttttg gtattcgtct tactactatt cataatttac attttctgct
     5761 aaaattaatg gaagatataa gacaagccat tcgagaagat cgtcttttag atttcaaaga
     5821 agaattcttc gagcaatatg gattaaatgt tgagaaccca aaaaactttt aagcaagagg
     5881 agcgtataaa atgcaatttt cattactaat atatatagtc gtaatttttg cggttatgta
     5941 tttcttgatg atcagaccac aacaaaaacg tgcgaaacag catcgtgagt tgattaataa
     6001 cattcaatct ggtcaaagaa ttacaactat tggtggtatt aaaggtactg ttaaagcagt
     6061 agatgaaaca actgttgtta ttacagttaa tggtcatggt actgaattaa ctttcgaaaa
     6121 acctgctatt aaacaagttg acccttcata acgagaagtg gtattaaaaa ggatgaaacg
     6181 aatagcgttt catccttttt aagttataag tagtatgtag aatagcttat ttttattcca
     6241 aaaaaagaaa ttgtga
//
LOCUS       DAOBBH010000144         6076 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.144, whole genome shotgun sequence.
ACCESSION   DAOBBH010000144 DAOBBH010000000
VERSION     DAOBBH010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.144"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(409..513)
                     /locus_tag="P6L01_002033"
     CDS             complement(409..513)
                     /locus_tag="P6L01_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031083.1"
                     /translation="MLTNRLTSVNYVKKRLIQNIQRILSKEVLYGEMQ"
     gene            608..1918
                     /gene="sbi"
                     /locus_tag="P6L01_002034"
     CDS             608..1918
                     /gene="sbi"
                     /locus_tag="P6L01_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-binding protein Sbi"
                     /protein_id="HDP3031084.1"
                     /translation="MKNKYISKLLVGAATITLATMISNGEAKASENTQQTSTKHQTTQ
                     NNYVTDQQKAFYQVLHLKGITEEQRNQYIKTLREHPERAQEVFSESLKDSKNPDRRVA
                     QQNAFYNVLKNDNLTEQEKNNYIAQIKENPDRSQQVWVESVQSSKAKERQNIENADKA
                     IKDFQDNKAPHDKSAAYEANSKLPKDLRDKNNRFVEKVSIEKAIVRHDERVKSANDAI
                     SKLNEKDSIENRRLAQREVNKAPMDVKEHLQKQLDALVAQKDAEKKVAPKVEAPQIQS
                     PQIEKPKAESPKVEVPQIQSPKVEVPQSKLLGYYQSLKDSFNYGYKYLTDTYKSYKEK
                     YDTAKYYYNTYYKYKGAIDQTVLTVLGSGSKSYIQPLKVDDKNGYLAKSYAQVRNYVT
                     ESINTGKVLYTFYQNPTLVKTAIKAQETASSIKNTLSNLLSFWK"
     gene            2454..3383
                     /gene="hlgA"
                     /locus_tag="P6L01_002035"
     CDS             2454..3383
                     /gene="hlgA"
                     /locus_tag="P6L01_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB subunit A"
                     /protein_id="HDP3031085.1"
                     /translation="MIKNKILTATLAVGLIAPLANPFIEISKAENKIEDIGQGAEIIK
                     RTQDITSKRLAITQNIQFDFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMI
                     WPFQYNISLKTKDSNVDLINYLPKNKIDSADVSQKLGYNIGGNFQSAPSIGGSGSFNY
                     SKTISYNQKNYVTEVESQNSKGVKWGVKANSFVTPNGQVSAYDQYLFAQDPTGPAARD
                     YFVPDNQLPPLIQSGFNPSFITTLSHERGKGDKSEFEITYGRNMDATYAYVTRHRLAV
                     DRKHDAFKNRNVTVKYEVNWKTHEVKIKSITPK"
     gene            3950..4897
                     /gene="hlgC"
                     /locus_tag="P6L01_002036"
     CDS             3950..4897
                     /gene="hlgC"
                     /locus_tag="P6L01_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgCB subunit C"
                     /protein_id="HDP3031086.1"
                     /translation="MLKNKILATTLSVSLLAPLANPLLENAKAANDTEDIGKGSDIEI
                     IKRTEDKTSNKWGVTQNIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKA
                     MRWPFQYNIGLKTNDKYVSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSF
                     NYSKSISYTQQNYVSEVEQQNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKD
                     PRDYFVPDSELPPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTH
                     YGNSYLDGHRVHNAFVNRNYTVKYEVNWKTHEIKVKGQN"
     gene            4899..5876
                     /gene="hlgB"
                     /locus_tag="P6L01_002037"
     CDS             4899..5876
                     /gene="hlgB"
                     /locus_tag="P6L01_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB/HlgCB subunit
                     B"
                     /protein_id="HDP3031087.1"
                     /translation="MKMNKLVKSSVATSMALLLLSGTANAEGKITPVSVKKVDDKVTL
                     YKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKATGNINSGFVKPNPNDYDFSKL
                     YWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNG
                     NTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSFHPTYGNEL
                     FLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMD
                     LYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK"
     gene            complement(5928..>6076)
                     /locus_tag="P6L01_002038"
     CDS             complement(5928..>6076)
                     /locus_tag="P6L01_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDP3031088.1"
                     /translation="VSRLLLSIILNLPVWVVLLNAIPGVIFTLIVAIPLYLTLRKRMA
                     VLLR"
BASE COUNT         2395 a         1020 c          897 g         1764 t
ORIGIN      
        1 gcgaatttta tgagctagaa aggcttatgc agttgacgtt ttacgtccaa ctgcgttcct
       61 tcgtcttctt caaatttatt tgttagaaag gcacccaact ctccctgttt gttaactttc
      121 gtctcgaaag tttctatgtt gggacccaat gcattagtag cgaagctatc taatgcgttc
      181 ctttattttc tgcgaatttt atgagctaga aagccgccca actcttcctg tttgtgaact
      241 ttcgcctcga aagtttctat gttgggaccc tatgcattag ttgcgaagct atctaatgtc
      301 gtgacctaat tatatagaag aaaaagtgca tcagtgacaa attaaatgag atttctactc
      361 taccaaactc tcttcgaaag acaatttcct cctctattta ttagcaaact attgcatttc
      421 tccatatagt acttccttac ttaaaatacg ctgaatgttc tgaattaaac gcttttttac
      481 atagttaaca ctagttaatc tattagttaa cattagttaa taattagtta atttccattt
      541 gtattctcat gtgataaatt ctaaaagcat acaataaatt taatatgtaa aaagaaaggg
      601 aatacacatg aaaaataaat atatctcgaa gttgctagtt ggggcagcaa caattacttt
      661 agctacaatg atttcaaatg gggaagcaaa agcgagtgaa aacacgcaac aaacttcaac
      721 taagcaccaa acaactcaaa acaactacgt aacagatcaa caaaaagctt tttatcaagt
      781 attacatcta aaaggtatca cagaagaaca acgtaaccaa tacatcaaaa cattacgcga
      841 acacccagaa cgtgcacaag aagtattctc tgaatcactt aaagacagca agaacccaga
      901 ccgacgtgtt gcacaacaaa acgcttttta caatgttctt aaaaatgata acttaactga
      961 acaagaaaaa aataattaca ttgcacaaat taaagaaaac cctgatagaa gccaacaagt
     1021 ttgggtagaa tcagtacaat cttctaaagc taaagaacgt caaaatattg aaaatgcgga
     1081 taaagcaatt aaagatttcc aagataacaa agcaccacac gataaatcag cagcatatga
     1141 agctaactca aaattaccta aagatttacg cgataaaaat aaccgctttg tagaaaaagt
     1201 ttcaattgaa aaagcaatcg ttcgtcatga tgagcgtgtg aaatcagcaa atgatgcaat
     1261 ctcaaaatta aatgaaaaag attcaattga aaacagacgt ttagcacaac gtgaagttaa
     1321 caaagcacct atggatgtaa aagagcattt acagaaacaa ttagacgcat tagtagctca
     1381 aaaagatgct gaaaagaaag tggcgccaaa agttgaggct cctcaaattc aatcaccaca
     1441 aattgaaaaa cctaaagcag aatcaccaaa agttgaagtc cctcaaatcc aatcaccaaa
     1501 agttgaggtt cctcaatcta aattattagg ttactaccaa tcattaaaag attcatttaa
     1561 ctatggttac aagtatttaa cagatactta taaaagctat aaagaaaaat atgatacagc
     1621 aaagtactac tataatacgt actataaata caaaggtgcg attgatcaaa cagtattaac
     1681 agtactaggt agtggttcta aatcttacat ccaaccattg aaagttgatg ataaaaacgg
     1741 ctacttagct aaatcatatg cacaagtaag aaactatgta actgagtcaa tcaatactgg
     1801 taaagtatta tatactttct accaaaaccc aacattagta aaaacagcta ttaaagctca
     1861 agaaactgca tcatcaatca aaaatacatt aagtaattta ttatcattct ggaaataatc
     1921 aatcaaaaat atcttctcta gttttacatc attttttaaa taattttcgt aataaaccgt
     1981 gattaaaaag aaccgctgat tctcaatcga atctgtggtt ctttttttat tttccatcaa
     2041 ttaaatgttt cttcgctatt tgtcagtcca cttctttacc tgcaacttgt taaataatcc
     2101 ttacatcgtt aacgaatagt tcatcattta gttgaatcag ctcaacttta ttaacttcaa
     2161 attttcacaa actattgcga aatccattcc tcttccacta caagcaccat aattaaacaa
     2221 caattcaata gaataagact tgcaaaacat agttatgtcg ctatataaac gcctgcgacc
     2281 aataaatctt ttaaacataa cataatgcaa aaacatcatt taacaatgct aaaaatgtct
     2341 cttcaataca tgttgatagt aattaacttt taacgaacag ttaattcgaa aacgcttaca
     2401 aatggattat tatatatatg aacttaaaat taaatagaaa gaaagtgatt tctatgatta
     2461 aaaataaaat attaacagca actttagcag ttggtttaat agccccttta gccaatccat
     2521 ttatagaaat ttctaaagca gaaaataaga tagaagatat cggtcaaggt gcagaaatca
     2581 tcaaaagaac acaagacatt actagcaaac gattagctat aactcaaaac attcaatttg
     2641 attttgtaaa agataaaaaa tataacaaag atgccctagt tgttaagatg caaggcttca
     2701 tcagctctag aacaacatat tcagacttaa aaaaatatcc atatattaaa agaatgatat
     2761 ggccatttca atataatatc agtttgaaaa cgaaagactc taatgttgat ttaatcaatt
     2821 atcttcctaa aaataaaatt gattcagcag atgttagtca gaaattaggc tataatatcg
     2881 gcggaaactt ccaatcagcg ccatcaatcg gaggcagtgg ctcattcaac tactctaaaa
     2941 caattagtta taatcaaaaa aactatgtta ctgaagtaga aagtcagaac tctaaaggtg
     3001 ttaaatgggg agtgaaagca aattcattcg ttacaccgaa tggtcaagta tctgcatatg
     3061 atcaatactt atttgcacaa gacccaactg gtccagcagc aagagactat ttcgtcccag
     3121 ataatcaatt acctccttta attcaaagtg gctttaatcc atcatttatt acaacattgt
     3181 cacacgaaag aggtaaaggt gataaaagcg agtttgaaat cacttacggc agaaacatgg
     3241 atgctacata tgcttacgtg acaagacatc gtttagccgt tgatagaaaa catgatgctt
     3301 ttaaaaaccg aaacgttaca gttaaatatg aagtgaactg gaaaacacat gaagtaaaaa
     3361 ttaaaagcat cacacctaag taaacagttc aatcatttta aaaaatcctg ggacacttca
     3421 tacttgtctc aggatttttt aacatattga atcagcctca taacattaaa ttaatttatc
     3481 gtacattaaa tttaataata acaactgatt tttataagaa caaagtatcg caccagagta
     3541 gatacacaaa taatacaaat gaatccattt aagttgaaag cttaaataaa tattctcaag
     3601 ttaataaata attaactttt agatggattc atcaaaaatc gtaaaaagaa caatttgtat
     3661 tttacaaaca ttaattaaaa ataaaagcaa gacattcgtg caatcggtta ccttaaattg
     3721 tttacaactg tcaacaatac caaggtttta ttaactatat ttctcacaaa attagctttt
     3781 agcattccaa acaaaaaagg ttaaattgaa cggaattatg gcatttttaa cttaattgta
     3841 aaaaaagttg ataatggtca attgttaatg aacagttaat tataataacg tccaaaatat
     3901 attattattt aattaagtta aataaaatta tagaaagaaa gtgaaactta tgcttaaaaa
     3961 taaaatatta gctacaactt tatctgtgag cttacttgcc cctcttgcca atccgttatt
     4021 agaaaatgct aaagctgcca acgatactga agacatcggt aaaggaagcg atatagaaat
     4081 tatcaaaagg acagaagata aaacaagtaa taaatggggc gtgactcaaa atattcaatt
     4141 tgattttgtg aaggataaaa aatataacaa agatgctctg atattaaaga tgcaaggatt
     4201 cattagctct agaacaacat attacaacta taaaaaaact aatcatgtta aagctatgcg
     4261 atggccattc caatataata ttggtttaaa aacaaatgat aaatatgttt ctttaattaa
     4321 ttatttacct aaaaataaaa ttgaatctac aaacgtgagt cagacattag gatacaatat
     4381 cggtggtaat ttccaatcag ccccatcact cggtggtaat ggatcattta actattctaa
     4441 atcgattagc tatacacaac aaaattatgt aagtgaagta gaacaacaaa actcaaaaag
     4501 tgttttatgg ggcgtcaaag cgaattcatt cgccactgaa tcaggtcaaa aatcagcatt
     4561 tgatagcgat ttatttgtag gctacaaacc tcatagtaaa gatcctagag attatttcgt
     4621 tccagacagt gagttacctc ctcttgtaca aagtggattt aacccttcat ttatcgccac
     4681 agtatctcat gaaaaaggtt caagcgatac aagcgaattt gaaattactt acggaagaaa
     4741 catggatgtc actcatgcca ttaaaagatc aacgcattat ggcaacagtt atttagacgg
     4801 acatagagtc cataatgcat tcgtaaatag aaactatact gttaaatacg aggtcaattg
     4861 gaagactcat gaaatcaagg tgaaaggaca gaattgatat gaaaatgaat aaattagtca
     4921 aatcatccgt tgctacatct atggcattat tattactttc tggtactgct aatgctgaag
     4981 gtaaaataac accagtcagc gtaaaaaaag tcgatgacaa agttacttta tacaaaacaa
     5041 cagccacagc agattctgat aagtttaaaa tttcacagat tttaacattt aatttcatca
     5101 aagataaaag ttatgataaa gatactttag tacttaaagc tactgggaat attaactcgg
     5161 gctttgtgaa acctaatcct aatgactatg acttttcaaa attatattgg ggagctaaat
     5221 acaatgtatc tataagctca caatctaatg attcagtaaa cgtcgttgat tatgcaccaa
     5281 aaaatcaaaa tgaagagttt caagttcaaa atactttagg ctatacattt ggtggtgaca
     5341 ttagtatctc taatggttta tctggtggac ttaatggaaa tacagctttt tctgaaacaa
     5401 ttaattataa acaagaaagt tacagaacaa cattaagtcg caacacaaat tataaaaatg
     5461 ttggctgggg agttgaagca cataaaatta tgaataatgg ttggggacct tatggaagag
     5521 atagcttcca cccaacatat ggtaatgaac tcttcttagc tggcagacaa agcagtgcat
     5581 acgctggcca aaacttcata gcgcaacacc aaatgccatt attatctaga agtaacttca
     5641 atccagaatt tttaagcgta ctatcacaca gacaagatgg cgctaaaaaa tctaaaatta
     5701 cagtaactta tcaacgtgaa atggatttat accaaattcg ttggaatggc ttctactggg
     5761 caggcgcaaa ttataaaaac tttaaaacta gaacatttaa atcaacatat gaaattgatt
     5821 gggaaaatca caaagtgaaa ttgttagata caaaagaaac tgaaaacaat aaatagctag
     5881 taaaacacgg tcgccaacag taattgtgac gaccgtgttt tgatttatta tcttagtaag
     5941 actgccattc tttttctcaa tgtgagatat aaaggaatag ctacaattaa agtgaatatt
     6001 acgcctggaa tcgcgtttaa caacactacc cacacaggta aatttaaaat aatagataat
     6061 agtagcctag atacca
//
LOCUS       DAOBBH010000145         6072 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.145, whole genome shotgun sequence.
ACCESSION   DAOBBH010000145 DAOBBH010000000
VERSION     DAOBBH010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.145"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(249..1622)
                     /locus_tag="P6L01_002039"
     CDS             complement(249..1622)
                     /locus_tag="P6L01_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HDP3031089.1"
                     /translation="MTAETNYFWLNCGYNRWNHNEPLVGQTALFESGAHFNPSQGFRA
                     FKKAKVGDQVIFYQVQTDTGLLGCGEIISVETGAQNKIRVQFRFNEQLKPLTADYLKR
                     SEALEFRMSNMKETLFNQITAEEFDLISGLGKGEIKIPRYFFLAETEEFEPGNQYTIY
                     THTYNGIKRNGYHFYTQLEEGDNIIFYNRTKNQSVVGIGEVSKHIHEKPPIPGRTNST
                     VIEVSYEKDITPITLSTLNKHPKLKNLYFLQENAKQAIASMSQAQYDAIIEMSDNNGL
                     KSPFEMVKKPDMLESEKEEALKPFILLVVDRKEEGLKAANDLLQKANANPVITTGHPD
                     FSEDMLYGKYLPNETGALYYREGFITQLMPKKDKSYLVIDNFNRIDTDIFQTYINVLE
                     GYEVTLPRYNKDGNMIKWSRQKDSFYYFNPNWHIVGITYDSLEEIKEKYSEQFLKYTR
                     IVKVKHD"
     gene            complement(2294..2524)
                     /locus_tag="P6L01_002040"
     CDS             complement(2294..2524)
                     /locus_tag="P6L01_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031090.1"
                     /translation="MKQFLYIALVCGVIAGLGAFLHIPQYPSMTIPRIVAILGIISAM
                     LTFKDKQISASLKFSALLINVLPLCGTFVASN"
     gene            complement(2555..3493)
                     /locus_tag="P6L01_002041"
     CDS             complement(2555..3493)
                     /locus_tag="P6L01_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HDP3031091.1"
                     /translation="MPLFLQPILKTKLWGGQRLSEFGYQLDNDTTGECWCVSAHPNGT
                     SEIINGPYQGQTLDRIWSEHRELFGDFPSKDFPLLTKIVDARESLSIHVHPDNSYAYE
                     HENGQYGKSECWYIIDAEEDAEIVIGTLAESREEVANHVQHGTIESILRYIKVKPGEF
                     YFIPAGTVHTISSGILAYETMQSSDITYRLYDFNRQDNQYNDRPLNIEKALDVIQYNA
                     PLPNILPESEIIENHKCTHIVSNDFFTLVKWEISGTLNYMKPREFCLVTVLEGEGQMI
                     VDGEIFKLTTGTNFILTSEDLDSVFEGDFTLMISYV"
     gene            complement(3929..4594)
                     /locus_tag="P6L01_002042"
     CDS             complement(3929..4594)
                     /locus_tag="P6L01_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDP3031092.1"
                     /translation="MNILVIGANGGVGSLLVQQLAKENVPFTAGVRQSDQLNALKSQG
                     MKAILVDVENDSIETLTETFKPFDKVIFSVGSGGNTGADKTIIVDLDGAVKSMIASKE
                     ANIKHYVMVSTYDSRRQAFDDSGDLKPYTIAKHYADDYLRRSGLNYTILHPGALTNAA
                     GSGKIEAAQYFDGKGEIPREDVATVLKEIVTSNHFNHQEFQIISGEQDIKDALTQFEI
                     EKD"
     gene            4746..5066
                     /gene="czrA"
                     /locus_tag="P6L01_002043"
     CDS             4746..5066
                     /gene="czrA"
                     /locus_tag="P6L01_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)-responsive metalloregulatory
                     transcriptional repressor CzrA"
                     /protein_id="HDP3031093.1"
                     /translation="MSEQYSEINTDTLERVTEIFKALGDYNRIRIMELLSVSEASVGH
                     ISHQLNLSQSNVSHQLKLLKSVHLVKAKRQGQSMIYSLDDIHVATMLKQAIHHANHPK
                     ESGL"
     gene            5068..6045
                     /gene="czrB"
                     /locus_tag="P6L01_002044"
     CDS             5068..6045
                     /gene="czrB"
                     /locus_tag="P6L01_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc efflux transporter CzrB"
                     /protein_id="HDP3031094.1"
                     /translation="MSHSHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLAN
                     SLALLSDGIHMFSDTFSLGVALVAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFVI
                     SILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAFL
                     HVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEGT
                     PSDVDIDEVITTIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLEN
                     IEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHNHHAHHHAHVH"
BASE COUNT         1944 a         1135 c          830 g         2163 t
ORIGIN      
        1 tggtcattcg tcttattcaa catacgactg gaaaactcat ataaagtttt aattcagtaa
       61 cataaattca tttatattca tatactgtct ttattattta ttaaaataaa aacgagcaat
      121 ctatcataag ctcttaatta gctcttatgg ttgattaccc gtcatttttc tttatgcggt
      181 tattcaaata tgcaattatg caatttttaa tcctatcctt acttcttttc aaaaactacc
      241 atatatcatt agtcatgttt gactttgact atacgtgtgt attttaaaaa ctgctctgaa
      301 tatttctcct taatttcctc taaactgtca tatgtaatac ctacaatatg ccaattagga
      361 ttgaaataat agaaagaatc tttttgtcgt gaccatttaa tcatattacc gtctttatta
      421 taacgtggta atgttacttc atagccttct aacacattaa tatacgtttg gaaaatgtct
      481 gtatctatac gattaaaatt atcaataact aaataacttt tatccttttt aggcattaat
      541 tgagtaataa acccttcacg atagtataat gcccctgttt catttggtaa atacttgcca
      601 taaagcatat cttctgaaaa atcaggatga cctgttgtta taacaggatt tgcattcgct
      661 ttttgcaata agtcattagc cgcttttaag ccctcttctt ttcgatctac aacaagtaaa
      721 ataaatggct ttaacgcttc ttctttttca ctttcaagca tatctggttt tttaaccatt
      781 tcaaatggag atttcaatcc attattatcg ctcatttcaa taattgcatc atactgtgct
      841 tgtgacatac ttgcaatagc ctgttttgca ttttcttgaa ggaaatataa atttttcaat
      901 ttaggatgct tatttaatgt acttaatgta atcggcgtaa tgtctttctc ataagacact
      961 tcaatcactg tactatttgt tctaccagga attggtggtt tttcatgaat atgctttgaa
     1021 acttctccaa ttccaacgac agattgattt ttcgttcgat tataaaaaat aatattgtcg
     1081 ccttcttcta actgagtata aaaatgataa ccattacgtt taataccgtt gtacgtgtgc
     1141 gtataaatcg tatattggtt tccaggttca aattcttcag tttcagctaa aaagaaataa
     1201 cgtggtatct taatttcgcc tttaccaaga ccacttatta aatcaaactc ttctgcagtg
     1261 atttggttga acaatgtctc tttcatatta ctcatacgaa attccaaagc ttcactacgc
     1321 tttaaataat cagctgttaa tggtttcaat tgttcattaa aacgaaactg tacacgtatt
     1381 ttattttgtg cacctgtttc aacactaata atttcaccac atccaagtag tccagtatcc
     1441 gtctgaactt gataaaagat gacttgatct cctactttag cctttttaaa cgctctaaat
     1501 ccttgagatg ggttaaaatg tgcgcctgat tcaaataaag ctgtttgtcc tactaacggt
     1561 tcattatgat tccaacggtt atatccacaa ttcaaccaaa aataattcgt ttctgctgtc
     1621 atcttaatac tccttaacct gaataaattt tagaaacact atgaattaca ttcttttagt
     1681 gtttcttatg cagttggacg cgtatgcgaa caactgtata ccctttgttc actgcgattt
     1741 taatcgcatt tcctataaca ttgtagcgcc caggacatta atttacgtcc cagaccctta
     1801 tcgttttcac ttctaagtaa gtcgaactat tttgctttac aacaagtgcg actctaaata
     1861 cagttggaca cacatacgag caactgtata ccttttaatc agttttctat attttattta
     1921 ttatatctgt cttaatgata aaaattgtta caaacagttt aacatattta gctacctatt
     1981 ttgcatataa aaaacatgct tgcatacact atgcaataaa aattacaaat ttatatatga
     2041 taccactatg cttgcttatc tctatagcgc cattgataca catttttaaa tatctatact
     2101 gccgttagaa ttttatcatg tcttaatttt cattaaatat taattacttc attttatata
     2161 aaccaacaaa aaccccctca ctactatgca agtgagaggt tatgttgatg tgctttattt
     2221 tcatcaatgt cattaacacg atcatatttc ttctgttaat gcactgtact ttattcaaac
     2281 gcgctatcga caattaattt gaagctacaa aggtaccgca taatggcagc acattaatta
     2341 acaatgcgct aaactttaat gaggcgctga tttgcttgtc tttaaaagtc aacatagcac
     2401 tgataattcc taaaatagct actatacgtg gaattgtcat gctcggatac tgcggtatat
     2461 gtaagaaagc accaagacct gctatcacac cacatactaa cgcaatatat agaaattgct
     2521 tcaaatcatt cactcctaaa ttgttattac actattacac atagctaatc atcaatgtga
     2581 aatcgccttc aaagacacta tccaaatctt cagaagtcaa aataaagttt gtaccagtag
     2641 tcagtttgaa aatttcacca tcgacaatca tttgtccttc accttccaac actgtaacta
     2701 aacagaactc tctaggcttc atataattta acgtgccaga aatttcccat ttaaccaatg
     2761 taaagaaatc attcgataca atgtgtgtac acttatggtt ttcaataatt tcactttcag
     2821 gcaaaatatt aggtaatggt gcattgtact gaataacgtc taaagctttt tcaatattta
     2881 acggtctatc attatattga ttatcttgac gattgaaatc ataaagtcta tatgtaatgt
     2941 ctgacgattg catcgtttcg tatgctaata ttcctgaaga aatagtatgt actgttcctg
     3001 ctggaataaa atagaattct ccaggtttta ctttaatata tctaagtatc gactctatcg
     3061 ttccgtgttg aacatgattc gcaacttctt ctctagactc tgctaatgtc cctataacta
     3121 tttctgcatc ttcttctgca tctataatat accaacattc agatttgcca tattgcccgt
     3181 tttcatgctc ataagcataa gaattatcag ggtgcacatg aatagaaagt gattctcttg
     3241 catccactat tttagttaga agcggaaaat ctttgcttgg gaaatcacca aacaattcac
     3301 gatgttctga ccaaatacgg tctaatgttt gaccttgata tggtccatta ataatctcgc
     3361 tcgtaccatt tggatgtgct gacacacacc aacattcccc agttgtatca ttgtctaatt
     3421 gatatccaaa ctcacttaga cgttgaccgc cccataattt tgtttttaaa attggttgta
     3481 aaaataatgg cattgttgca cctccattgt gattaagtaa gcaatagaac tctgatgttg
     3541 ttgttccatt atattttgat tttgttctca tttacatcgt attataaact tccacatttc
     3601 aaattaacta ttagtgattg taccatattt actaacattg cagtactgcc aattaaagcg
     3661 cttcacttaa atttacagta ctttaacatt tttaaaaatt aatagcatag agattatctc
     3721 tctcttacat ttgtacacat ttccctttaa atttactcgc ccattatacc aattaataaa
     3781 caactttaat agttgtgcca tacattgttc aaattctttg taaaatgcat agacaatacg
     3841 tacttattca tacttataat tcatcatttt caaaaaaata acgagttacg aaaacgtaac
     3901 ccgcttcaaa tcatatttac tatccttatt aatccttttc aatttcaaat tgagttaaag
     3961 catctttaat gtcctgctca ccactaataa tttgaaactc ttggtgatta aaatgattgg
     4021 atgtgacaat ttcttttaat actgtcgcaa catcttctct aggaatttca cctttaccat
     4081 caaaatattg tgcagcttct atctttccag atcctgctgc atttgtaagt gcccctggat
     4141 gtaaaattgt ataattcaaa cctgaacgtc ttaaatagtc atcagcgtaa tgtttagcta
     4201 ttgtatatgg ctttaaatca ccgctatcat caaaagcctg acgtctcgaa tcatatgttg
     4261 aaaccatgac atagtgttta atattagcct ctttactcgc aatcattgat ttaacagcac
     4321 catctaaatc gacaataatt gttttatctg cacccgtgtt ccctccagaa cctactgaaa
     4381 agataacttt atcgaatggt ttaaacgtct cagttaaagt ctctattgaa tcattttcaa
     4441 catcaacaag aattgctttc ataccttgtg attttaacgc attaagttga tctgattgcc
     4501 taacaccagc agtaaatggt acattttctt ttgctaattg ttgcactagt aacgaaccta
     4561 caccgccatt agcacctata accaaaatat tcatttacaa cactctccta tttattattc
     4621 tctatgccat accactttat gagatatgta aaacttgtta caactataaa aatcaattga
     4681 catagtactg ggaacgtatt aaattaatat atgaacaaat attcatatga aaggattgtc
     4741 atataatgtc agaacaatat tcagaaataa atacagatac attagaacgc gtaactgaaa
     4801 ttttcaaggc attaggcgat tacaatcgaa tacgtatcat ggaattgtta tcagtcagtg
     4861 aagcaagtgt tggtcacatt tcacatcaat tgaatttatc tcaatcaaat gtctcgcacc
     4921 aattaaaatt acttaaaagt gtgcatcttg tgaaagcaaa acgacaaggc caatcaatga
     4981 tttattcatt agatgacatc cacgtagcaa ctatgttaaa gcaagccata catcacgcga
     5041 atcatcctaa agaaagtggg ttataatatg tctcattcac atcatcatga ccatatgcat
     5101 agtcatgtaa ctacaaataa taagaaagta ttgtttatat cgtttttaat aatcggtcta
     5161 tatatgttta tcgaaatcat cggcggtctc cttgctaaca gcttggcatt actatctgac
     5221 ggtatccata tgtttagcga cacattctca ttaggtgttg cacttgtcgc atttatttat
     5281 gctgaaaaga atgccacaac tacaaaaaca tttggttata aacgtttcga agtactcgca
     5341 gcgttattta acggtgtaac gctttttgta ataagtattt tgattgtttt tgaagcgatt
     5401 aaacgtttct ttgttccttc tgaagttcaa tcaaaagaaa tgttaatcat tagtattatc
     5461 ggtttaattg tcaatatcgt tgttgcattc tttatgttta aaggcggcga cacttcacac
     5521 aatttaaata tgcgtggtgc ttttctacat gttatcggag acttattagg ttcagttggc
     5581 gccattactg cagctatttt aatttgggca tttggatgga caatcgccga tcctatcgca
     5641 agtattttag tttccgttat tattttaaaa agtgcttggg gtatcacaaa atcttcaatt
     5701 aacattttaa tggaaggcac accaagtgat gttgatatag atgaagttat aactactatt
     5761 aaaaaggatt cacgaataca aagtgtgcat gattgccatg tttggacaat ttcaaatgat
     5821 atgaatgcat tgagttgtca tgttgttgta gaccatacat tgacaatgaa agaatgtgaa
     5881 ttattattag aaaacattga gcatgattta ttacatttaa atattcacca tatgactatt
     5941 caattagaaa cgcctaatca caaacatgat gaatcgatta tatgttcagg aacacatagt
     6001 cattcacata accatcatgc tcatcatcac gcgcatgtac attaataatt ttaacctact
     6061 gccattgcat cg
//
LOCUS       DAOBBH010000146         6060 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.146, whole genome shotgun sequence.
ACCESSION   DAOBBH010000146 DAOBBH010000000
VERSION     DAOBBH010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.146"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            55..315
                     /locus_tag="P6L01_002045"
     CDS             55..315
                     /locus_tag="P6L01_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HDP3031095.1"
                     /translation="MYYEIGEIIRKNIHVNGFDFKLFILKGHMGISIQVKDMNNVPIK
                     HAYVVDENDLDMASDLFNQAIDEWIEENTDEQDRLINLVMKW"
     gene            325..546
                     /locus_tag="P6L01_002046"
     CDS             325..546
                     /locus_tag="P6L01_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2483 family protein"
                     /protein_id="HDP3031096.1"
                     /translation="MKQTVTYIIRHRDMPIYITNKPTDNNSDVSYSTNRNRAREFNGM
                     EEASINMDYHKAIKKTVTETIEYEEVEHD"
     gene            539..1162
                     /locus_tag="P6L01_002047"
     CDS             539..1162
                     /locus_tag="P6L01_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDP3031097.1"
                     /translation="MTEQTLFEQLNSKNVNDHTEQKNGLTYLAWSYAHQELKKIDPNY
                     TVKVHEFPHPDINTENYFVPYLATPEGYFVQVSVTVKDSTETEWLPVLDFRNKSLAKG
                     SATTFDINKAQKRCFVKASALHGLGLYIYNGEELPSASDNDITELEERINQFVNLSQE
                     KGRDATIDKTMRWLKISNINKLSQKQIAEAHQKLDAGLKQLDSEEKQ"
     gene            1162..1590
                     /gene="ssb"
                     /locus_tag="P6L01_002048"
     CDS             1162..1590
                     /gene="ssb"
                     /locus_tag="P6L01_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDP3031098.1"
                     /translation="MLNRAVLVGRLTKDPELRSAPNGVNVGTFTLAVNRTFTNAQGER
                     EADFINVVVFKKQAENVKNYLSKGSLAGVDGRLQTRSYENKVGQRVFVTEVVADSVQF
                     LEPKNNNQQPNNNYHQQRQTQTGNNPFDNTTAITDDDLPF"
     gene            1604..2278
                     /locus_tag="P6L01_002049"
     CDS             1604..2278
                     /locus_tag="P6L01_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031099.1"
                     /translation="MPKITSYITQDDGTTTVVISDVELGNKETLLLDNGFDVEVDVNV
                     IDPFQITGKQRRKIFALVKDIEEHTGQPMDYMRHMFIEFVRTYYGYDERISLSNCTRT
                     QASQIIEATLDWTFYNDIPLSYKTSNLLKQDKSFLYWSTVNRNCVICGKPHADLAHYE
                     AVGRGMNRNKMNHYDKHVLALCREHHNEQHAIGVKSFDDKYQLHDSWIKVDERLNKML
                     KGEKKE"
     gene            2275..2424
                     /locus_tag="P6L01_002050"
     CDS             2275..2424
                     /locus_tag="P6L01_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031100.1"
                     /translation="MNRLRIIKIALLIVILAEEIRSAKKIKKFTPEDSKGFPDITKDS
                     IKEPK"
     gene            complement(2417..2698)
                     /locus_tag="P6L01_002051"
     CDS             complement(2417..2698)
                     /locus_tag="P6L01_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031101.1"
                     /translation="MEKPYMLTYDLNSPGQKYEELRNVIKKEISNGHCNYWKSSFLFR
                     SSLSTSEMIEKLKPYLDSGDKLFVTEIVNNKQGWLTKEQWDFINHNIFI"
     gene            2764..3534
                     /locus_tag="P6L01_002052"
     CDS             2764..3534
                     /locus_tag="P6L01_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein"
                     /protein_id="HDP3031102.1"
                     /translation="MATFRVYKESGNFVTVHKDFIHDSNISWKAKGILLYLLSRPDNW
                     QIYETELEQHSTDGLSGLKSGIKELEEIGYIQRSRKRDKSGRLNGYEYLVYEQPHHIR
                     FSNVGKTVNGKTNNGKTVNGKSHTTNNNSTNNDLTNNNNTNNEGSILSGNPTVSSIPY
                     KEIIEYLNKKAGKHFKHNTAKTKDFIKARWNQDFRLEDFKKVIDIKTAEWLNTDSDKY
                     LRPETLFGSKFEGYLNQKIQPTGTNQLERMKYDESYWD"
     gene            3544..4323
                     /locus_tag="P6L01_002053"
     CDS             3544..4323
                     /locus_tag="P6L01_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDP3031103.1"
                     /translation="MKPLFSEKINESLKKYQPTHVEKGLKCERCGSEYDLYKFAPTKK
                     HPNGYEYKDGCKCEIYEEYKRNKQRKINNIFNQSNVNPSLRDATVKNYKPQNEKQVHA
                     KQTAIEYVQGFSTKEPKSLILQGSYGTGKSHLAYAIAKAVKAKGHTVAFMHIPMLMDR
                     IKATYNKNAVETTDELVRLLSDIDLLVLDDMGVENTEHTLNKLFSIVDNRVGKNNIFT
                     TNFSDKELNQNMNWQRINSRMKHNARKVRVIGDDFRERDAW"
     gene            4317..4475
                     /locus_tag="P6L01_002054"
     CDS             4317..4475
                     /locus_tag="P6L01_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031104.1"
                     /translation="MVTKEFLKIKLECSDMYAQKLIDEAQGDENKLYDLFIQKLAERH
                     TRPAIVEY"
     gene            4488..4709
                     /locus_tag="P6L01_002055"
     CDS             4488..4709
                     /locus_tag="P6L01_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3269 family protein"
                     /protein_id="HDP3031105.1"
                     /translation="MPKEKYYLYREDGTEDIKVIKYKENENEVYSLTGAHFSDEKKIM
                     TDSDLKRFKGAHGLLYEQELGLQATIFDI"
     gene            4719..5066
                     /locus_tag="P6L01_002056"
     CDS             4719..5066
                     /locus_tag="P6L01_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDP3031106.1"
                     /translation="MSKYNAKKVEYKGIVFDSKVECEYYQYLESNMNGTNYDRIEIQP
                     KFELQPKFGKQRPITYIADFSLWKDGKLVEVLDVKGKATEVANIKAKIFRYQYRDVNL
                     TWICKAPKYTGKT"
     gene            5128..5313
                     /locus_tag="P6L01_002057"
     CDS             5128..5313
                     /locus_tag="P6L01_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001187261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HDP3031107.1"
                     /translation="MQQQAYINATIDIRIPTEVEYKHFGDVDNEKDALADYLYNNPNE
                     ILEYDNLKIRNVNIEVE"
     gene            5314..5619
                     /locus_tag="P6L01_002058"
     CDS             5314..5619
                     /locus_tag="P6L01_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031108.1"
                     /translation="MARITKETKTVSDGYSREDRETTLNYDYENQEWIAYSSVPTHIT
                     RMTKLYGDDVEVLERLESGTAVLVRAKLPKSAIGFRKLMSEERRQELSERAKRAFGH"
     gene            5747..>6060
                     /locus_tag="P6L01_002059"
     CDS             5747..>6060
                     /locus_tag="P6L01_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031109.1"
                     /translation="MSIVKINGKPYKFTEHENELIKKNGLTPGMVAKRVRGGWALLEA
                     LHAPYGMRLAEYKEIVLSKIMERESKEREMARQRRKEAELRRKKPHLFNVPQVHPRGR
                     YA"
BASE COUNT         2399 a          955 c         1197 g         1509 t
ORIGIN      
        1 gcacttaaga aaaatttcaa gttaaatata aaatgaaaaa cggaggaagt caagatgtat
       61 tacgaaatag gcgaaatcat acgcaaaaat attcatgtta acggattcga ttttaagcta
      121 ttcattttaa aaggtcatat gggcatatca atacaagtta aagatatgaa caacgtacca
      181 attaaacatg cttatgtcgt agatgagaat gacttagata tggcatcaga cttattcaac
      241 caagcaatag atgaatggat tgaagagaac acagacgaac aggacagact aattaactta
      301 gtcatgaaat ggtaggaggt cgctatgaag cagactgtaa cttacatcat ccgtcatagg
      361 gatatgccaa tttatataac taacaaacca actgataata attcagatgt tagttactcc
      421 acaaatagaa atagagctag ggaatttaac ggtatggaag aagcgagtat caatatggat
      481 tatcacaaag caatcaagaa aacagtgaca gaaactattg agtacgagga ggtagaacat
      541 gactgaacaa acattatttg aacagttgaa cagtaaaaac gtgaatgatc atacagaaca
      601 aaaaaatgga ttaacttatc tagcatggtc atatgcacac caagagctga aaaagattga
      661 cccaaactac acagtaaaag tacacgagtt tccacatcca gatattaaca cagaaaatta
      721 ttttgtacct tatttggcta caccagaagg ctattttgta caggtatctg tgactgtgaa
      781 agatagtaca gagactgagt ggcttccagt attggacttt agaaataaat cgcttgctaa
      841 aggtagtgca acaactttcg atattaacaa agcgcaaaaa cgatgttttg ttaaagcttc
      901 ggctttacac ggtttaggct tatatatcta caacggcgag gaactaccaa gtgcaagtga
      961 caacgatatt acagaattag aagagcgtat caatcagttc gtgaacttat ctcaagaaaa
     1021 agggcgagat gcaactatcg ataaaacgat gagatggcta aaaatatcta acattaataa
     1081 attaagtcaa aaacaaatcg cagaagcaca ccaaaaatta gatgcgggat taaaacaatt
     1141 ggatagtgag gagaaacaat aatgttaaac agagcagtat tagtaggacg cttaacaaaa
     1201 gacccagaat taagaagcgc gccaaatggc gtaaatgtag gtacattcac attggcagta
     1261 aacagaacat tcacgaatgc tcaaggcgag cgtgaagcag attttataaa cgtagtagtg
     1321 ttcaagaaac aagctgaaaa tgttaaaaac tacctttcta aagggtcgct ggcaggtgta
     1381 gacgggcgac tacaaacacg tagctacgaa aataaagtcg ggcaacgtgt atttgtgaca
     1441 gaagtagtag cggacagtgt tcaattctta gaaccgaaga ataacaacca acaaccaaac
     1501 aacaattatc atcaacaaag acaaactcaa actggtaata atccttttga taataccact
     1561 gcgattactg atgatgactt accgttctga ttggaatgat taaatgccga aaattactag
     1621 ttatatcact caagacgacg gcacaacaac agttgtcatc tctgatgttg aattaggcaa
     1681 taaagaaaca ttactacttg ataacgggtt tgatgtagaa gtagatgtaa acgttataga
     1741 tccgtttcaa attaccggca agcaacgtcg aaaaatattc gcgcttgtca aagacataga
     1801 agaacataca ggtcaaccaa tggactatat gagacatatg ttcatcgagt ttgtaagaac
     1861 gtactacggc tatgatgaac gtatttcgct aagtaattgt acgagaacac aagcaagtca
     1921 aatcattgaa gcaacgcttg actggacgtt ctacaatgac ataccactta gctacaaaac
     1981 gagtaatcta ctgaaacaag ataaatcatt cttatactgg tcaactgtta accgcaactg
     2041 tgtaatatgc ggaaagcctc acgctgacct agcgcattac gaagcagtag gtagaggcat
     2101 gaacagaaac aagatgaatc actacgacaa acatgtatta gcgttatgtc gcgaacatca
     2161 taacgagcaa catgcgattg gtgttaagtc atttgatgat aaatatcaat tgcatgactc
     2221 gtggataaaa gttgatgaga ggctcaataa aatgctgaaa ggagagaaaa aggaatgaat
     2281 agactaagaa taataaaaat agcactccta atcgtcatct tggcggaaga gattagaagc
     2341 gctaaaaaaa ttaaaaaatt tacccctgag gattctaaag gttttcctga tataacaaaa
     2401 gattcaataa aagaacctaa ataaaaatat tatggttgat aaaatcccat tgttcttttg
     2461 ttaaccaccc ttgtttgtta ttgactattt ctgtaacaaa cagcttatct ccagaatcga
     2521 gataaggttt caacttttct atcatttctg aagttgataa agaagaacgg aataaaaatg
     2581 aagatttcca ataattgcaa tgaccattag aaatttcctt ttttataaca tttctcaatt
     2641 cctcatattt ttgtccgggt gagtttaaat catatgttaa catataaggt ttttccatat
     2701 tttattcacc cccaatctaa cgcagtagcg ataacaaaat tataccagaa aggagataac
     2761 gaaatggcaa catttagagt ttacaaagaa tcaggtaact ttgtcacagt acacaaagat
     2821 tttatacatg attctaatat aagttggaag gctaaaggta ttctacttta tttgttaagt
     2881 cgacctgata actggcaaat ttacgaaaca gaactagagc aacattcaac tgatggactt
     2941 agcggtttaa agagtggaat caaggaactg gaagaaattg gatacattca acgtagtaga
     3001 aaacgtgata aaagtggtag gttaaatggt tatgagtact tagtatatga gcaaccgcac
     3061 cacattcgat tttccaacgt tggaaaaacc gttaacggta aaaccaacaa tggaaaaacc
     3121 gttaatggta aatcgcatac tactaataat aatagtacta ataatgattt aactaataat
     3181 aacaatacta ataatgaagg aagtatattg tcgggcaacc cgacggtgtc ttccattccc
     3241 tataaagaaa ttatcgaata cttaaataaa aaagcaggaa agcattttaa acataataca
     3301 gctaaaacaa aagattttat taaagcaaga tggaatcaag attttaggtt ggaggatttt
     3361 aaaaaggtga ttgatatcaa aacagctgaa tggttaaaca cggatagcga taaatacctt
     3421 agaccagaaa cactttttgg cagtaaattt gaggggtacc tcaatcaaaa aatacaacca
     3481 actggcacga atcaattgga acgcatgaag tacgacgaaa gttattggga ttagggggat
     3541 attatgaaac cactattcag cgaaaagata aacgaaagct tgaaaaaata tcaacctact
     3601 catgtcgaaa aaggattgaa atgtgagaga tgtggaagtg aatacgactt atataagttt
     3661 gctcctacta aaaaacaccc gaatggttac gagtataaag acggttgcaa atgtgaaatc
     3721 tatgaggaat ataagcgaaa caagcaacgg aagataaaca acatattcaa tcaatcaaac
     3781 gttaatccgt ctttaagaga tgcaacagtc aaaaactaca agccacaaaa tgaaaaacaa
     3841 gtacacgcta aacaaacagc aatagagtac gttcaaggct tctctacaaa agagccaaaa
     3901 tcattaatat tgcaaggttc atacggaact ggtaaaagcc acctagcata cgctatcgca
     3961 aaagcagtta aagctaaagg gcatacggtt gcttttatgc acataccaat gttgatggat
     4021 cgtatcaaag cgacatacaa caaaaatgca gtagagacta cagacgagct agtcagattg
     4081 ctaagtgata ttgatttact tgtactagat gatatgggtg tagaaaacac agagcacact
     4141 ttaaataaac ttttcagcat tgttgataac agagtaggta aaaacaacat ctttacaact
     4201 aactttagtg ataaagaact aaatcaaaat atgaactggc aacgtatcaa ttcaagaatg
     4261 aaacacaatg caagaaaagt aagagtaatc ggagacgatt tcagggagcg agacgcatgg
     4321 taaccaaaga atttttgaaa attaaacttg agtgttcaga tatgtacgct cagaaactca
     4381 tagacgaggc acagggcgat gaaaataagt tatatgacct atttatccaa aaacttgcag
     4441 aacgtcatac acgccccgct atcgtcgaat attaaggagt gttaaaaatg ccgaaagaaa
     4501 aatattactt ataccgagaa gatggcacag aagatattaa ggtcatcaag tataaagaga
     4561 atgagaatga agtttattcg ctcacaggag cccatttcag cgacgaaaag aaaattatga
     4621 ctgatagtga cctaaaacga tttaaaggcg ctcacggact tctatatgag caagagctag
     4681 gtttacaagc aacgatattt gatatttaga ggtggacgat gagtaaatac aacgctaaga
     4741 aagttgagta caaaggaatt gtatttgata gcaaagtaga gtgtgaatat taccaatatt
     4801 tagaaagtaa tatgaatggc actaactatg atcgtatcga aatacaaccg aaattcgaac
     4861 tacaacctaa atttgggaaa caaagaccga ttacgtatat agctgatttc tctttgtgga
     4921 aggatggcaa actggtcgaa gttttagatg ttaaaggtaa ggcgactgaa gttgccaaca
     4981 tcaaagcgaa gatattcaga tatcagtata gagatgtgaa tttaacgtgg atatgtaaag
     5041 cacctaagta cacaggcaaa acatagatta cttacgagga attaattaaa gcaagacgag
     5101 aacgcaaaag agaaatgaag tgatctaatg caacaacaag catatataaa cgcaacgatt
     5161 gatataagaa tacctacaga agttgaatat aagcattttg gtgatgtgga taacgaaaaa
     5221 gatgcgctgg cagattactt atataacaat cctaacgaaa tactagaata tgacaattta
     5281 aaaattagaa acgtaaatat agaggtggaa taaatggcaa gaattaccaa agaaacaaaa
     5341 actgtaagcg acggttattc aagagaagac cgagaaacga cattgaacta tgattacgaa
     5401 aatcaagaat ggattgctta ctcatcggta ccgacacata ttactagaat gacaaagttg
     5461 tacggcgatg atgtagaggt attggaacga ttagaatctg ggactgcggt attggttagg
     5521 gcgaaactac ctaaaagcgc aataggtttt agaaaattaa tgtctgaaga gcgacgacaa
     5581 gaattatctg agagagcaaa aagagctttt ggtcattagt gctcgtgaat atagggcgaa
     5641 aaacgaccaa aaagacacac taatactttt taggataaat aacatccgga gaaaaaaaca
     5701 tgagctttaa aaattttaac acaggataaa tacagaggtg gaataaatga gtatcgtaaa
     5761 gattaacggt aaaccatata aatttaccga acatgaaaat gaattgataa aaaagaatgg
     5821 tttaactcca ggaatggttg caaaaagagt acgaggtggc tgggcgttgt tagaagcctt
     5881 acatgcacct tatggtatgc gcttagctga gtataaagaa attgtgttat ccaaaatcat
     5941 ggagcgagag agcaaagaac gtgaaatggc taggcaacga cgtaaagagg ctgagctaag
     6001 aagaaagaag ccacatttgt ttaatgtgcc acaagtgcat ccaagaggac gttatgcgtg
//
LOCUS       DAOBBH010000147         6054 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.147, whole genome shotgun sequence.
ACCESSION   DAOBBH010000147 DAOBBH010000000
VERSION     DAOBBH010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.147"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(98..1879)
                     /gene="recQ"
                     /locus_tag="P6L01_002060"
     CDS             complement(98..1879)
                     /gene="recQ"
                     /locus_tag="P6L01_002060"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDP3031110.1"
                     /translation="MMQQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSI
                     CYQVPGLLLGGTTIVISPLISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQRIEKALSN
                     GEIQFLYVAPERFENRYFLNMLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFT
                     LPQDFTIIALTATATVEVQQDIREKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFIL
                     DYIKTHDEDAGIIYCSTRKQVEELQEALESQKIESVIYHAGLSNKEREEAQNDFLFDR
                     VKVVVATNAFGMGIDKSNVRFVIHYNMPGDLESYYQEAGRAGRDGLKSECILLFSERD
                     INLHEYFITVSQADDDYKDKMGEKLTKMIQYTKTKKCLEATIVHYFEPNEKLEECEQC
                     SNCVQQDKSYNMTQEAKMIISCIARMKQQESYSVIIQVLRGESTDYIKYKGYDQISTH
                     GLMKGYTTSELSHLIDELRFKGFLNENDEILMCDTSIKKLLSNEVEVFTTPFKQKATE
                     KVFINTVEGVDRVLFSQLVEVRKKLSDKLTIAPVSIFSDYTLEEFAKRKPASKQDMIN
                     IDGVGSYKLKHYCPAFLETIQNYKAKV"
     gene            complement(1891..3774)
                     /locus_tag="P6L01_002061"
     CDS             complement(1891..3774)
                     /locus_tag="P6L01_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDP3031111.1"
                     /translation="MGMEAYKIEHLNKSYADKTIFDNLDLSISEGEKIGLVGINGTGK
                     STLLKVIGGIDDDFTANVMHPNQYRIRYSSQKQDLNEDMTVFDAVLSSDTTTLRIIKQ
                     YEQAVQAYADDQSDKLFKRMIDAQDAMDQHDAWDYNAEIKTILSKLGIHDTTKYIKEL
                     SGGQQKRVVLAKTLIEQPDLLLLDEPTNHLDFESISWLINYVKQYPHTVLFVTHDRYF
                     LNEVSTRIIELNRGKLASYPGNYESYIEMRAEREVTLQKQQQKQRALYKEELAWMRAG
                     AKARTTKQQARINRFNDLENEVNQQYKDDKGELNLAYSRLGKQVFELEDLSKAINDKV
                     LFEHLTEIIQKGERIGVVGPNGAGKTTLLNILSGEDQQFEGKLKTGQTVKVAYFKQTD
                     ETLDRDIRMIDYLREESEVAKEKDGTSVSITQLLERFLFPSATHGKKVYKLSGGEQKR
                     LYLLRLLVHQPNVLLLDEPTNDLDTETLTILEDYIHTFGGTVITVSHDRYFLNKVAQS
                     YWFIHDGQMEKIIGTFEDYESYKKSLDKNKSTLKQQSKSYTTVRKKNGLSYKEKLEYE
                     QLMKRIEQAEVRMEEIDVLMIEASADYGKIKELNEEKEQLEIQYDLDITRWSELEEIK
                     EQQ"
     gene            complement(4044..5984)
                     /gene="ltaS"
                     /locus_tag="P6L01_002062"
     CDS             complement(4044..5984)
                     /gene="ltaS"
                     /locus_tag="P6L01_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyglycerol-phosphate lipoteichoic acid
                     synthase LtaS"
                     /protein_id="HDP3031112.1"
                     /translation="MSSQKKKISLFAFFLLTVITITLKTYFSYYVDFSLGVKGLVQNL
                     ILLMNPYSLVALVLSVFLFFKGKKAFWFMFIGGFLLTFLLYANVVYFRFFSDFLTFST
                     LNQVGNVESMGGAVSASFKWYDFVYFIDTLVYLFILIFKTKWLDTKAFSKKFVPVVMA
                     ASVALFFLNLAFAETDRPELLTRTFDHKYLVKYLGPYNFTVYDGVKTIENNQQKALAS
                     EDDLTKVLNYTKQRQTEPNPEYYGVAKKKNIIKIHLESFQTFLINKKVNGKEVTPFLN
                     KLSSGKEQFTYFPNFFHQTGQGKTSDSEFTMDNSLYGLPQGSAFSLKGDNTYQSLPAI
                     LDQKQGYKSDVMHGDYKTFWNRDQVYKHFGIDKFYDATYYDMSDKNVVNLGLKDKIFF
                     KDSANYQAKMKSPFYSHLITLTNHYPFTLDEKDATIEKSNTGDATVDGYIQTARYLDE
                     ALEEYINDLKKKGLYDNSVIMIYGDHYGISENHNNAMEKLLGEKITPAKFTDLNRTGF
                     WIKIPGKSGGINNEYAGQVDVMPTILHLAGIDTKNYLMFGTDLFSKGHNQVVPFRNGD
                     FITKDYKYVNGKIYSNKNNELITTQPADFEKNKKQVEKDLEMSDNVLNGDLFRFYKNP
                     DFKKVNPSKYKYETGPKANSKK"
BASE COUNT         1862 a         1089 c          833 g         2270 t
ORIGIN      
        1 tgaataatag gtaatctcca gttaagaaat agtgttattt taccttgaat tcaaaaaaac
       61 acccagtaaa acaaggaatg cttactaggt gccttcacta tactttggct ttataatttt
      121 gaatcgtttc taaaaatgct ggacaataat gttttaattt gtaactacct acgccatcaa
      181 tattaatcat atcttgtttc gaagcaggct tacgtttagc aaattcctcc aacgtgtaat
      241 cagaaaatat acttacaggt gctatcgtta atttgtcact taacttttta cgaacttcta
      301 ccaactgact gaataatact cggtcaaccc cttcaaccgt atttataaat actttttcag
      361 tcgctttttg cttaaatggt gttgtgaata cttctacttc attactgagt aattttttaa
      421 ttgaagtatc acacattaat atttcgtcat tttcatttaa gaaccctttg aatcttaatt
      481 catctattaa gtgacttaat tctgatgttg tgtaaccttt cattaaacca tgggttgaaa
      541 tttggtcata acctttatac ttaatataat ctgttgactc tcctcttaac acttgaatga
      601 taacactata actctcttgt tgtttcatac gagcgatgca actaataatc atcttagctt
      661 cttgtgtcat attatatgat ttatcttgtt gaacacaatt actacattgt tcacattctt
      721 ctaatttttc attcggttca aaataatgga caattgttgc ttctagacat ttttttgttt
      781 ttgtatattg aatcatttta gttaactttt cgcccatttt atctttatag tcatcatcag
      841 cttgagagac tgttataaaa tactcgtgta aattgatatc gcgttcgcta aataacaaaa
      901 tacattcact tttcaacccg tcacgacctg cacgacccgc ttcttgataa taagattcta
      961 aatctccagg catattataa tgaataacaa aacgtacatt ggatttatca atacccatac
     1021 caaaagcatt tgtagcaacg actactttaa cacgatcaaa taagaaatca ttctgcgctt
     1081 cttctctttc tttattgctc aaacctgcat gatatataac actttcaatt ttctgacttt
     1141 ctaaggcttc ttgaagctct tcaacttgct tacgtgtaga acaataaata atacctgcat
     1201 cttcatcatg tgttttaata taatccaata taaatttttg acgttgataa gtaggattta
     1261 ctttaaaaat taagtttcta cgcttagtac tcgttttaat ttgatcagtt tgagcgatat
     1321 ttaacttttc tctaatatct tgctgtactt caaccgtggc agttgctgtc aacgctatta
     1381 ttgtaaaatc ttgaggtaac gtaaatactt ttgaaataac attttggtaa ctcggcctga
     1441 aatcatgacc ccatttagaa atacaatgcg cttcatcaaa cgcgactaag tgaatcttta
     1501 tacgctgaag catatttaaa aaatatcggt tttcaaatcg ttctggtgca acatacaaaa
     1561 attgaatttc tccatttgat aatgcttttt caatacgttg ttgctctttt tgagtcaaac
     1621 tactatttaa aaaagcagct tgaattccca tcgcttttaa ttgatccact tgatctttca
     1681 ttaatgatat tagtggactt attacaattg ttgtaccacc taacaataaa cctggtactt
     1741 gatagcatat agacttacct ccaccagttg gtaagacacc aagcacatta cgatggtcta
     1801 atactttgct aataatttct tcttgtcctg gtcgaaatgt ttcataccca aagtaatgcg
     1861 ataatgtttg ttgcatcata aattgacccc ttattgttgt tctttaattt cttctaactc
     1921 actccatctt gtgatgtcta aatcatattg aatttcaagt tgttcttttt cttcgtttag
     1981 ttctttaatt ttcccataat ctgcacttgc ctcaatcatg agcacatcaa tttcttccat
     2041 tcttacttcc gcttgttcta tgcgtttcat caattgttca tattctaatt tttctttata
     2101 tgataaacca tttttcttac gtacagttgt ataagattta gattgttgct tcaatgtgga
     2161 tttattttta tctaatgatt ttttataact ttcataatct tcaaaagttc cgataatctt
     2221 ttccatctga ccatcatgaa taaaccaata tgactgtgca actttattta agaagtagcg
     2281 atcatggctt acggtaatca ctgtaccacc gaaagtatga atataatctt caagtattgt
     2341 taaagtctca gtatctaaat catttgtcgg ttcatctaac aacagaacat ttggctggtg
     2401 tacgagtaga cgtaataaat acaaacgctt ttgctctcca ccagataatt tataaacttt
     2461 tttaccatga gttgcacttg gaaataaaaa tcgttcaaga agttgtgtaa tagataccga
     2521 ggttccatct ttttcttttg cgacctcact ttcttctctt aaataatcaa tcatacgaat
     2581 atctctatcc agggtctcat ctgtttgctt aaaataagct actttaaccg tctgcccagt
     2641 cttcaattta ccttcgaatt gttggtcttc tccactcaaa atatttaaga gtgttgtttt
     2701 accagctcca tttggcccaa caacaccaat acgctcacct ttttgaataa tttccgtcag
     2761 atgttcaaat aatactttat cattaatagc ctttgataag tcttctaatt cgaacacttg
     2821 cttacctaat cttgaataag caagattcaa ttcaccttta tcgtctttat attgctggtt
     2881 aacttcattt tctaggtcat taaatcgatt aattctagct tgttgctttg tagtacgagc
     2941 cttagctcca gccctcatcc aagcaagttc ttccttatat aaagctcgtt gcttttgttg
     3001 ttgcttttga agtgttactt ctctttcagc gcgcatttca atataagatt catagttacc
     3061 aggatatgac gctaacttac ctctgtttag ttcaataatt ctagtggaaa cttcatttaa
     3121 aaaatatcga tcatgggtta cgaataaaac tgtatgagga tattgcttca cataattgat
     3181 caaccagctg attgattcga agtctaaatg gttcgtaggt tcatctaata acaataaatc
     3241 tggttgttct attaatgttt tagcaagtac aacacgtttt tgttgtccgc cggataattc
     3301 tttaatgtat ttagtagtat catgtatacc tagttttgag aggattgttt taatttcagc
     3361 gttatagtcc caagcatcat gttgatccat agcatcttgc gcatctatca ttcgcttgaa
     3421 caatttatca ctttggtcat ccgcataagc ttgtactgcc tgctcatatt gcttgatgat
     3481 gcgtaaagtt gttgtatcag aacttaatac tgcatcaaaa actgtcatat cttcattaag
     3541 gtcctgtttc tgagacgaat atcgaattcg atattgattt ggatgcataa cattggctgt
     3601 aaaatcatca tcaataccac caattacttt taacaacgta cttttccctg taccatttat
     3661 gcccaccaaa cctatttttt caccttctga aattgataaa tctaggttat cgaatatagt
     3721 cttatcggca taagatttat ttaaatgttc aattttatat gcttccatac ccatcttcct
     3781 tcttctgttg aaatctctac gtacttaaat cagttctata cattatacac ctttttgaaa
     3841 tcaaaagaaa tcgataccgt taagtgtcat aatttaatat attgttgctt caactataaa
     3901 taaaacttga taaggctttg atgactatgc taatgatttt tataatatac ctttttatca
     3961 acacatatac tgtaaaacat aatataaaag aacataataa aacccggatt gatattttat
     4021 caatccgagt tcgtgtttaa atattatttt ttagagtttg ctttaggtcc tgtttcatat
     4081 ttatacttcg aaggatttac ctttttgaag tctggatttt tgtagaatct aaacaaatca
     4141 ccattaagca cgttgtcact catttcgaga tccttttcaa cttgcttttt attcttttcg
     4201 aaatcagctg gttgagtagt tatgagttca ttatttttat tagaataaat cttaccatta
     4261 acatatttat aatcttttgt tataaagtca ccatttctga atggaactac ttgattatga
     4321 cctttagaga ataaatcagt accgaacatt aaataattct tcgtatctat accagccaaa
     4381 tgtaaaattg ttggcattac atcgacttga ccagcatatt cattattgat accaccagat
     4441 ttaccaggga ttttaatcca gaaaccagtt ctgtttaaat ctgtaaattt agctggtgtg
     4501 attttttcac ctaatagttt ttccatggca ttgttatggt tttcagagat accatagtgg
     4561 tcaccataaa tcataatcac tgaattgtca tataatcctt ttttcttcaa gtcattaata
     4621 tattcttcta atgcttcgtc taaataacgc gctgtttgaa tataaccatc aactgttgca
     4681 tcacctgtgt ttgacttctc aatagtcgca tccttttcat ctaatgtgaa tggatagtgg
     4741 ttagtcaatg taattaaatg agaatagaat ggtgatttca tcttagcttg ataattagca
     4801 gaatctttaa agaaaatttt gtctttcaag cctaagttta caacgttttt atctgacatg
     4861 tcatagtatg ttgcatcata gaatttatcg ataccaaagt gtttatatac ttggtctctg
     4921 ttccagaatg ttttatagtc accgtgcatg acatcagatt tgtagccttg cttttgatct
     4981 aaaattgctg gtaatgactg atacgtatta tctcctttta atgaaaaggc agaaccttgc
     5041 ggtaaaccgt ataaactgtt atccattgta aattcagagt cagatgtttt accttgacct
     5101 gtttgatgga aaaagttagg gaagtatgtg aattgctctt tcccacttga taatttgttt
     5161 aaaaacggtg ttacttcttt accattaacc tttttattaa ttaagaaggt ttggaaactt
     5221 tctaaatgaa tcttaataat atttttcttc tttgccaccc cataatattc tgggttaggc
     5281 tctgtttgac gttgtttcgt ataatttaat acttttgtta agtcatcttc agatgctaac
     5341 gctttttgtt gattattttc gatagtttta acaccatcgt atactgtaaa gttataaggt
     5401 cctaaatatt tcactaaata tttatggtca aatgtacgtg ttaataattc tggtctgtca
     5461 gtttcagcaa aagctaagtt taagaagaat aatgctactg aagctgccat tacgacagga
     5521 acaaatttct tactaaatgc ttttgtgtct aaccattttg ttttaaatat taaaatgaat
     5581 aagtaaacta acgtatcaat gaaataaaca aagtcatacc atttgaatga tgcactaacc
     5641 gcaccaccca tagattctac gttacctact tggtttaaag tactaaacgt taaaaaatca
     5701 gagaagaatc taaagtacac aacattggca tataatagga acgtcaataa gaagccgcct
     5761 atgaacatga accaaaatgc ttttttgcct ttaaagaata ggaacacact taaaaccagt
     5821 gctactaaac tataaggatt catcaataat attaagtttt gtactaaacc tttaacacct
     5881 aaagaaaaat caacataata agaaaaatac gtcttcaagg taatcgttat tacggttaat
     5941 aagaagaacg caaaaagact aattttcttt ttttgtgaac tcatgattct ttcccccgtt
     6001 atttagataa taaatcttgc gaaagtactt gaataaatta gatattaaca ttat
//
LOCUS       DAOBBH010000148         6028 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.148, whole genome shotgun sequence.
ACCESSION   DAOBBH010000148 DAOBBH010000000
VERSION     DAOBBH010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.148"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..685)
                     /locus_tag="P6L01_002063"
     CDS             complement(<1..685)
                     /locus_tag="P6L01_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HDP3031113.1"
                     /translation="MTVEERSNTAKVDILGVDFDNTTMLQMVENIKTFFANQSTNNLF
                     IVTANPEIVNYATTHQAYLELINQASYIVADGTGVVKASHRLKQPLAHRIPGIELMDE
                     CLKIAHVNHQKVFLLGATNEVVEAAQYALQQRYPNISFAHHHGYIDLEDETVVKRIKL
                     FKPDYIFVGMGFPKQEEWIMTHENQFESTVMMGVGGSLEVFAGAKKRAPYIFRKLNIE
                     WIYRALIDWK"
     gene            921..1673
                     /locus_tag="P6L01_002064"
     CDS             921..1673
                     /locus_tag="P6L01_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HDP3031114.1"
                     /translation="MNYLSNFFNTTIQLNIYIIVIVAACYIAIHQYRHKPVLNYLDVI
                     LNYIPVLTHEFGHVLFNKLAGGRAKDLVIVTSPRERQQTLQQGFAITQSRHLAGQWLT
                     TIGGYLMPPIMLLIGLASSHYQIPSFFIFTYLLIFIYFLILTSRKGSPIVVITLISIM
                     LYFILKDENIVEIQLLVTMSYQYILGVLLGEVLQSSWTIAKLTFQRPSPQWDGSALKE
                     LSHVPIFIYSTIWIIFNLYAVNILLKYTHLIN"
     gene            complement(1753..2397)
                     /locus_tag="P6L01_002065"
     CDS             complement(1753..2397)
                     /locus_tag="P6L01_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDP3031115.1"
                     /translation="MLTEEKEDYLKAILTNNGDKNFVTNKILSQFLNIKPPSVSEMVG
                     RLEKAGYVETKPYKGVRLTEDGLTHTLDIIKRHRLLELFLIEILKYNWEEVHQEAEIL
                     EHRISDLFVERLDSLLNFPETCPHGGVIPRNNEYKEKYITTILNYEPGDIVTIKRVRD
                     KTDLLIYLSSKDISIGNEVEIVSKDEMNKVIIIKRNDNVIIVSYENAMNMFAEK"
     gene            2519..3262
                     /locus_tag="P6L01_002066"
     CDS             2519..3262
                     /locus_tag="P6L01_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDP3031116.1"
                     /translation="MLETKDLNLFLGNKHVLKNISLSIPVRGEIIGIMGPNGAGKSSL
                     IKSLIGEFNATGTKLLYNKPIQQQLQHITYIPQKAHIDLDFPISVEQVILSGCYKEIG
                     WFRRPNKSARDKLKQLLSDLELESLRHRQISELSGGQLQRVLVARALMSESEVYFLDE
                     PFVGIDFSSEKLIMTKIENLKQQGKLILIIHHDLSKAKQYFDRIILLNQTLRYFGDSE
                     EAMSVTRLNETFMSSTDCSDPSQRSNITC"
     gene            3256..4092
                     /locus_tag="P6L01_002067"
     CDS             3256..4092
                     /locus_tag="P6L01_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDP3031117.1"
                     /translation="MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMG
                     DAMSHAVLPGVALSFLFGIPMFVGALITGMIASIFIGYITSSSKTKPDAAIGISFTAF
                     LASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMIST
                     FDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKL
                     YSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGS
                     LT"
     gene            4089..5018
                     /locus_tag="P6L01_002068"
     CDS             4089..5018
                     /locus_tag="P6L01_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDP3031118.1"
                     /translation="MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYD
                     MAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADIILYNGLNLETGNGWFEKA
                     LEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFI
                     DNDKKHKADYEKQGNKYIAQLEKLNNDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGI
                     TPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFG
                     EVYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK"
BASE COUNT         2022 a         1005 c          911 g         2090 t
ORIGIN      
        1 gtttccaatc tattaatgct ctatatatcc attcaatgtt taattttcta aatatataag
       61 gcgctctctt tttagcccca gcaaatactt caagagaacc acctacgccc atcatcactg
      121 tagattcaaa ttggttttca tgtgtcataa tccattcttc ttgtttaggg aatcccatac
      181 ctacaaatat gtaatcaggt ttaaacagtt taattcgttt tactactgtc tcatcttcta
      241 aatcaatata accgtgatga tgtgcaaacg atatgtttgg atatctttgt tgcaatgcat
      301 attgtgccgc ttctacaact tcattagttg cccctagcaa aaatactttt tgatgattta
      361 catgagcaat tttcaaacat tcatccatca actcaatacc aggtatacga tgcgctagag
      421 gttgctttaa acgatgcgaa gctttgacta ctcctgtccc atcagcaaca atatagctcg
      481 cttgatttat taactctaaa tacgcttgat gtgtcgtcgc gtaattcact atttcagggt
      541 tggctgttac tataaaaaga ttattcgttg attgatttgc aaaaaaggtt ttaatatttt
      601 caaccatttg caacattgtt gtattatcaa aatcgacccc taaaatgtca actttggctg
      661 tattggatct ttcttcaaca gtcataacag gaatctccta tcttatttca ttattatgtt
      721 agtgtaatca atattaacag tatttatatt catcctattt atatcgaaat gattttgctt
      781 tattttaaca catattcacc ttttcaaaaa ttaaaattag tttaactaag gtaagtctaa
      841 agtctaacgc tattgatgcc aatatcattt ataataagtg ctaagcaaac tttaaattaa
      901 tatcttatat gaggttaaca atgaattatt tatcaaactt ttttaatacg acaattcaac
      961 ttaatatata cattatcgtt attgttgcag cttgttatat tgctattcat caatatagac
     1021 ataagcctgt tttgaactat ttagatgtca ttttaaacta tatccctgtt ttaacacatg
     1081 aatttggtca tgtactgttt aataaactcg ctggtggccg tgcaaaagat ttagtcattg
     1141 taacaagtcc acgagagcga caacagacat tacaacaagg attcgcaatt acacaatcta
     1201 gacatctagc aggccaatgg cttacaacga ttggtggtta tttaatgcca cctattatgt
     1261 tattgatagg tttagcgtcg agccattatc agatacctag tttctttata tttacctatt
     1321 tattgatatt tatttatttt ttaattttaa catctagaaa aggctcaccc attgttgtta
     1381 taactttaat ttctatcatg ttgtatttta tattaaaaga tgaaaatata gtagaaattc
     1441 aactacttgt cacaatgagt tatcaatata ttttaggtgt attattaggc gaagttctac
     1501 aatctagttg gacgattgct aaattgactt tccagcgtcc gagtccacag tgggatggta
     1561 gtgcacttaa agaactcagt catgtaccta tcttcattta tagtacaatc tggattatct
     1621 tcaatcttta tgcagtgaat attttactca aatatactca cttgataaat taactaataa
     1681 aaaagaagcc taaagcaatt atccatatgt ctttatcagt tcaatcatgg atatctttat
     1741 ggcttcttta ttttattttt cagcaaacat gttcattgca ttttcgtaac tgacaataat
     1801 tacattatca ttacgtttaa tgataattac tttattcatt tcatctttcg atacaatttc
     1861 cacttcatta ccaatagaaa tatctttact agacaaatat attagcaaat cggtcttatc
     1921 tctcacacgt ttgattgtaa cgatatcacc aggttcataa ttcaaaatcg ttgttatata
     1981 tttctcttta tattcattat ttctaggaat cacaccgccg tgcgggcaag tttctgggaa
     2041 atttaacagg ctatccagcc tttcaacaaa taaatctgaa attcgatgtt ctaaaatttc
     2101 tgcttcttga tgtacttctt cccaattata tttcaatatt tctattaaaa ataattctaa
     2161 tagtcgatgt ctcttaatga tatcaagcgt atgcgttaaa ccatcctctg ttaatctaac
     2221 acctttgtat ggttttgttt caacatagcc tgctttttca agacgtccta ccatttcact
     2281 tacagatgga ggcttaatat ttaaaaattg agataagatt ttatttgtca caaagttttt
     2341 atcgccatta ttcgtaagga ttgcctttaa atagtcctct ttttcttcag ttaacatact
     2401 ttcacctcac atacattgtc tatattatat cacgaattta cttgacatga taaatattct
     2461 cagtttatta tacaattaat taggttagcc taaactttta attaggaggt ataaacgttt
     2521 gttagaaaca aaagatttaa atctgttttt aggtaataag catgtactta aaaacatttc
     2581 cttatcgata ccagtacgcg gcgaaataat tggtatcatg ggcccgaatg gtgctggtaa
     2641 atcttccctt atcaagtctt taattggtga atttaatgct accggtacta aattgttata
     2701 taacaaacct atacaacaac aactgcaaca tattacatat attccacaaa aagcacatat
     2761 tgatttagat tttcctataa gtgtggaaca agtgatttta tcaggttgct acaaagaaat
     2821 tggatggttt agacgaccta ataaatcagc aagggataaa ctcaaacagt tattaagcga
     2881 tttagaatta gaatctttac gtcatcgaca aatttcagaa ttaagtggtg gacaattaca
     2941 acgtgtgcta gtagcaagag cattgatgtc cgaaagtgaa gtttattttc tagatgagcc
     3001 gtttgtcgga attgatttta gtagcgaaaa attaatcatg acaaaaatcg agaacttaaa
     3061 acaacaagga aaacttattc ttatcatcca ccatgatcta tcaaaagcaa agcaatactt
     3121 tgatcgcatt attctattaa atcaaacatt acgatacttt ggtgatagtg aagaggctat
     3181 gagtgtcact cgcttaaacg aaacatttat gagtagcact gactgtagtg accctagtca
     3241 aaggagcaat ataacatgtt agagtttgtc gaacatttat ttacatatca attcttgaat
     3301 cgagcattga taacttcaat tattgtaggg atagtttgtg gtacagttgg tagtttaatt
     3361 gtattacgtg gtctttcatt aatgggagat gcaatgagtc acgcagtatt acctggtgtt
     3421 gccctatcat tcttatttgg tattccaatg tttgtaggcg cacttataac tggtatgatc
     3481 gcaagtattt ttatcggtta tatcacatct agtagtaaaa cgaaacctga cgccgctatt
     3541 ggaattagtt ttaccgcttt cttagcttcg gggattatta ttattagctt aataaacact
     3601 acgacagatt tgtaccatat tctgtttggt aatttactgg caatcacaaa tagtgcattt
     3661 ttaacaacta ttgtgattgg ttcaatcgtc cttattctaa tcattatttt ctatcgtcca
     3721 ttaatgattt ctacattcga tccaacgttt agtagaatga gtggtctaaa tacgacgtta
     3781 ttacattact ttgtgatgtt gttactctca ttagtaacag tagcaagtat tcaaacggta
     3841 ggtattattc ttgtagttgc attattaatt acacctgcat ctacagcatt tttaattagt
     3901 aaaaaactat actcaatgat gattatagca agtttaataa gtgtcataag ttcaattgtc
     3961 ggactttatt acagctatat ttacaacatt cctagtggtg cgacaatcgt actttgtacg
     4021 tttgtaattt atatcatcac attatttttc acaaaattta cgaatagaaa gaaacgagga
     4081 agtttaacat gaaaaaatta gtacctttat tattagcctt attacttcta gttgctgcat
     4141 gtggtactgg tggtaaacaa agcagtgata agtcaaatgg caaattaaaa gtagtaacga
     4201 cgaattcaat tttatatgat atggctaaaa atgttggtgg agacaacgtc gatattcata
     4261 gtattgtacc tgttggtcaa gatcctcatg aatatgaagt taaacctaaa gatattaaaa
     4321 agttaactga cgctgacatt attttataca acggattaaa tttagagact ggtaacggtt
     4381 ggtttgaaaa agccttagaa caggctggta aatcattaaa agataaaaaa gttatcgcag
     4441 tatcaaaaga tgttaaacct atctatttaa acggtgaaga aggcaacaaa gataaacaag
     4501 atccacacgc atggttaagt ttagataatg gtattaaata cgtaaaaaca attcaacaaa
     4561 catttatcga taacgacaaa aaacataaag cagattatga aaagcaaggt aacaaataca
     4621 ttgctcaatt ggaaaaatta aataatgaca gtaaagacaa atttaatgac attccaaaag
     4681 aacaacgtgc catgattaca agtgaaggtg ccttcaagta cttctcaaaa caatacggta
     4741 ttacaccagg ttatatttgg gaaattaaca ctgaaaaaca aggtacacct gaacaaatga
     4801 gacaagctat tgagtttgtt aaaaagcaca aattaaaaca cttattagta gaaacaagtg
     4861 ttgataagaa agcaatggaa agtttatctg aagaaacgaa gaaagatatc tttggtgaag
     4921 tgtacacaga ttcaatcggt aaagaaggca ctaaaggtga ctcttattac aaaatgatga
     4981 aatcaaatat tgaaactgta cacggaagca tgaaataaca cgctgtgttt taatgaagta
     5041 agatgaattg atgttgatgc aacctaaaat attggtatct ccaatatttt aggctacaca
     5101 tcaacataac aaagtcgaag gctaatagtc ccatatcgtg cgttaaatat atattaccct
     5161 cctattaata tatataccgt tcccgatcgc acgatatggt ggtattagaa cttctctttg
     5221 aacgaaagag aaaagctaga agttcttatg cagttttaat taaactgtaa acatttgtca
     5281 ctctttaaat caaagagtaa agttaaaagc tttatgtggt tttgattaaa ctgcgaacag
     5341 ctgcttctct ttgaacgaaa gagaaaagct agaagttctt atgcagtttt aattaaactg
     5401 tcgttccctt catctctttt aaccacagag atgcgttaga agttcttcta atacaattta
     5461 tacaacgcca ttccctacac actcttataa aagagattca cgcgcgtcaa taaattgtat
     5521 tacatactaa ctaaaaagct tttcttaatc gtactaacga agttagaggt tcttatgtga
     5581 ttggacaact gttctcccta ctagactttt agcatagctg gttaaaagta ctattacaga
     5641 ttgttcaatt gtatgcccct ttcctttgaa cggaatatta aaagctatgt gcagtaactt
     5701 ttaataggca taccactttt gcatcaagct ataaataatc gcatactttg atatttaatc
     5761 tggcctatca aagtaaacat ctatggattc ctccccccat agatccttaa gccacactca
     5821 tccgatgtca tatgagtgtg gctatttttt gtgaatgcac ttatcttcat ctttaaatga
     5881 cttcgattaa tctatctcct ccaattttta aaacttattt caattaaatc tcaaatatta
     5941 ataattcgta tagtttaaaa ctatctattc aattccccta aaataaaagg gattgatgaa
     6001 aagcccttca gtttttcagg ttcttcat
//
LOCUS       DAOBBH010000149         5770 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.149, whole genome shotgun sequence.
ACCESSION   DAOBBH010000149 DAOBBH010000000
VERSION     DAOBBH010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.149"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..200
                     /locus_tag="P6L01_002069"
     CDS             <1..200
                     /locus_tag="P6L01_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDP3031119.1"
                     /translation="KNFNPSESDTFIYCMSGQITLNLGKEIYQAQEEDVLYFKARDNH
                     RLSNESNNETRILIVATASYL"
     gene            213..1307
                     /locus_tag="P6L01_002070"
     CDS             213..1307
                     /locus_tag="P6L01_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3031120.1"
                     /translation="MEPLLSLKSVSKSYDDLNILDDIDIDIESGYFYTLLGPSGCGKT
                     TILKLIAGFEYPDSGEVIYQNKPIGNLPPNKRKVNTVFQDYALFPHLNVYDNIAFGLK
                     LKKLSKTEIDQKVTEALKLVKLSGYEKRNINEMSGGQKQRVAIARAIVNEPEILLLDE
                     SLSALDLKLRTEMQYELRELQSRLGITFIFVTHDQEEALALSDFLFVLKDGKIQQFGT
                     PTDIYDEPVNRFVADFIGESNIVEGRMVRDYVVNIYGQDFECVDMGIPENKKVEVVIR
                     PEDISLIKAEEGLFKATVDSMLFRGVHYEICCIDNKGYEWVIQTTKKAEVGSEVGLYF
                     DPEAIHIMVPGETEEEFDKRIESYEEVDNA"
     gene            1300..2097
                     /locus_tag="P6L01_002071"
     CDS             1300..2097
                     /locus_tag="P6L01_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3031121.1"
                     /translation="MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNY
                     QQIFTTKYLKMFAYSILYAALITIITLAISYPAAYYITRSKFQNILLMIMIIPTWINL
                     LLKTYAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSM
                     KAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGN
                     KVINIGTAIEEQFLTIQNYGMGSTIAIFLIVFMAFILIITKSSNGRG"
     gene            2103..2912
                     /locus_tag="P6L01_002072"
     CDS             2103..2912
                     /locus_tag="P6L01_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3031122.1"
                     /translation="MKWYGKLYIGILLAILYIPIFFLMFYSFNSAGNMIHFEHFTLEH
                     YQSLFQNDHLMSVIFNTIAVALLAASISTVIGTFGAIAIYYLRNKKFKVTLLTLNNVL
                     MVSSDVVIGASFLIMFTTIGHFTGLGLGFWTVLISHIAFCIPIVVIIVLPQLYEMNNN
                     MLNAARDLGATEPQLLSNIIIPNILPSIIGGFFMALTYSLDDFTVSFFVTGNGFSVLS
                     VEVYAMARKGISMEINAISTLLFAVIVLGILGYYLIQYVINKKKLIKRGVK"
     gene            2912..3985
                     /locus_tag="P6L01_002073"
     CDS             2912..3985
                     /locus_tag="P6L01_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HDP3031123.1"
                     /translation="MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGE
                     YIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHL
                     LLPIDHNKVPNIKNLDSDYMNMSFDKGNKYSLPYFFGTVGILYNKEKYPNESFDSWKS
                     LYNPKFKNQILLVDGAREIIGMSLNKLGYNLNDRNSHHLKEAERDLTKLAPQVRGVVG
                     DEITMMLQQNEGNIAVVWSGVAAPLVQEGDKYNYVIPKEGSNLWFDNMVIPKTAQNKE
                     GAYKFMNFLLDAKNNKQNTEFVGYATPNKAARQLLPKEIKDDHRFYPTKKEQERLEVY
                     KDLGPEVLSEYNENFLNFKMSLK"
     gene            4059..5078
                     /locus_tag="P6L01_002074"
     CDS             4059..5078
                     /locus_tag="P6L01_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDP3031124.1"
                     /translation="MTGEQFTQIKRPVSRLTEKVLGWLCWVMLLVLTVITMFIALVSF
                     SNNTSIANLENTLNNNAFIQQLLAGNGYNTTQFVIWLQNGIWAIIVYFIVCLLISFLA
                     LISMNIRILSGFLFLISAIVTIPLVLLIVTLIIPILFFIIAMMLFIRKDKVEMVAPQY
                     YEEYNGPIYDYREPVYERPQPKDDYYDVPKYEKELDKSNTVYDQEQERDKYDQFPKRA
                     VESEYNHDERTEEEPSVLSRQAKYKQKSTEELGIEDDGYYAEPEVDPKELKAQQKREK
                     AEIKAKKKEKRKAYNQRMKERRKNQPSAVSQRRMNFEERRQIYNNDISEERNSSEVKD
                     KKEQE"
     gene            5361..>5770
                     /locus_tag="P6L01_002075"
     CDS             5361..>5770
                     /locus_tag="P6L01_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDP3031125.1"
                     /translation="MNRKPELIMAWIANSISIIYLLVMGLSYFSLKSGNASQREELAK
                     QLSQNGGNVSLDMLQTTMGALAIILLISTLYGIFATICIKGRRKLSIILFVIAIIVSL
                     MALNLIAIVLWVIVMIMLISKKESKETTHKDDEY"
BASE COUNT         2070 a          741 c          998 g         1961 t
ORIGIN      
        1 ataaaaattt taatccatca gagtctgata cgtttattta ttgtatgtca ggtcagataa
       61 cacttaattt aggcaaagag atatatcaag cacaagaaga agacgttttg tattttaaag
      121 cacgagataa tcatcgtttg tcaaacgaat caaacaatga aacacgaata cttattgtag
      181 cgacagcttc atatttatag gggggatctt atttggaacc gttattatca ttaaaatcag
      241 ttagtaaaag ctatgatgat cttaatatct tagatgacat agatattgat attgaatcag
      301 gatactttta tacattatta ggtccttcag gttgtggtaa aacaacaatt ttaaaattaa
      361 ttgcagggtt tgaatatcct gacagtggtg aagtgattta tcaaaacaaa ccaattggta
      421 atttaccacc aaataaacgt aaagtgaata cagtctttca agattatgca ttatttccac
      481 acttaaacgt atatgataat atcgcttttg gtttgaaatt aaaaaaatta tcaaaaaccg
      541 aaattgatca aaaagtaact gaggcattaa aattagtaaa actttcaggt tatgaaaaaa
      601 gaaatattaa tgaaatgagt ggcggacaaa agcaacgtgt tgcaattgca cgtgctatcg
      661 taaatgaacc agaaatatta ttgttagatg aatctttatc cgcattagat ttgaaattgc
      721 gtactgaaat gcaatatgaa ttacgagaat tgcaatctag attaggtatt acatttatat
      781 ttgtaacaca tgatcaagaa gaagcgttag cattaagtga ctttcttttt gtattaaaag
      841 atgggaaaat tcaacaattt ggcacaccaa cagatatata tgacgaacca gtgaatcgat
      901 ttgtagctga ttttattgga gaatctaata ttgttgaagg acgcatggtt agagattatg
      961 tcgtgaatat ttatgggcaa gatttcgaat gtgtcgatat gggtattcct gaaaataaaa
     1021 aagtagaagt cgttattcga ccagaagata tatctttaat caaagctgaa gaaggattat
     1081 ttaaagcaac tgttgattct atgttattta gaggggtcca ctatgaaata tgttgtatag
     1141 acaataaagg ttatgaatgg gtaatacaaa cgactaaaaa agctgaagta ggcagtgaag
     1201 ttggtcttta ttttgatcct gaagccattc atattatggt tcctggagaa acagaagaag
     1261 aatttgataa acgtattgaa agctatgagg aagtagacaa tgcgtaatac taataaattt
     1321 ctcttaatcc cgtatttact atggatggtt atatttatta ttgtaccagt tgtattactc
     1381 atttattttt catttttaga tatcaatgga cattttagtt tcacgaatta tcaacaaatt
     1441 tttactacaa aatatttgaa aatgtttgca tattcaattt tatatgccgc tttaataaca
     1501 attattacgt tggctatcag ttatccagct gcctattata ttactcgttc gaaatttcaa
     1561 aatatcttat taatgataat gattattcca acatggataa atttattgtt aaagacatat
     1621 gcttttatag gtttattaag tcatgatggc gtgattaatc aatttttcca cttatttaat
     1681 ttaccatcat tcaatttgtt atttacaact ggtgcgtttt tagtagtggc aagttacatt
     1741 tatataccat ttatgatttt acctatattt aatagcatga aagcaattcc taataattta
     1801 ttgcaggcct caagtgattt aggtgccagt cctttctata cttttagaaa agtaatcatg
     1861 ccgttaacaa aagaaggtgt tatgactggg attcaagtaa cctttattcc atcactttca
     1921 ttatttatga ttacaagatt aattgcaggt aataaagtca taaatatagg tacggcaata
     1981 gaggaacaat ttttaacaat tcaaaattat ggtatgggat caactatagc tatattctta
     2041 attgtattta tggcattcat tttaatcatt acaaaatcat ctaatgggag agggtgaatc
     2101 atatgaaatg gtatggaaag ctgtatatcg ggatactttt agcgatttta tacatcccaa
     2161 tattcttttt aatgttctat tcattcaatt cggctggtaa tatgattcac tttgaacatt
     2221 ttacattaga gcattatcaa tcattatttc aaaatgatca tttaatgtcg gtcattttta
     2281 atacgatagc tgtagcactt ttagcagcct caatttctac agttattggt acatttggtg
     2341 ccattgctat ttattattta agaaataaaa agtttaaagt aactttacta acattgaata
     2401 atgtcttgat ggtatcatcc gacgttgtca taggtgcatc attcttaatt atgtttacaa
     2461 cgattggcca ttttactggt cttggtttag gattttggac agttctaata tctcatattg
     2521 cattttgcat acctatagtt gtgattatcg tcttaccaca actgtatgaa atgaataata
     2581 atatgttaaa tgctgcaaga gatttaggag cgactgaacc acaattatta agcaacatta
     2641 ttattcctaa tattttacct tctattatag gaggtttctt tatggcttta acttattcac
     2701 tagacgattt cacagtaagt ttcttcgtta ctggtaatgg ctttagtgtg ttatcagttg
     2761 aagtttatgc tatggcgaga aaaggaatta gtatggaaat taatgcgatt tcaacattat
     2821 tgtttgctgt tattgtatta ggaatactag gatattattt gattcaatac gtgataaata
     2881 agaaaaaact aatcaagcga ggtgtaaaat aatgaaacgt tttttacaac tcattatagg
     2941 tgcattagtt gtgggtatgc tttgtcttac tttaagtcat tggtttaaat ctaaagaaca
     3001 agtgcataca aatcaaaaaa tttacgtata caattggggc gaatatattg atccagagtt
     3061 aattaagaaa tttgaaaaag aaactggcat tcaagtcgtt tatgaaactt tcgattcaaa
     3121 tgaagcgatg gaagccaaaa ttcgcaatgg cggtacacat tatgatgttg cttttcctag
     3181 tgaatataca gttcaaaaat tgaaaagaga tcatttattg ttaccaatag atcataataa
     3241 ggtacctaat attaaaaatt tagattcaga ttatatgaat atgtcatttg ataaaggcaa
     3301 taaatattct ttaccttatt tctttggaac tgtaggtatt ttatataata aagaaaagta
     3361 tccaaatgaa tcatttgata gttggaagtc attgtataat cctaaattta aaaaccaaat
     3421 tttactagtt gacggtgcta gagagattat aggcatgagt ttgaataaac ttgggtataa
     3481 tcttaatgac cgtaattcgc accatttaaa agaagcagag cgagatttaa ccaaactagc
     3541 accacaagta agaggtgtcg taggtgatga aattaccatg atgcttcaac aaaatgaagg
     3601 taacatagcg gttgtttgga gtggtgttgc agcacctcta gtgcaagaag gggataaata
     3661 taattatgtt attcctaaag aaggatcgaa tttatggttc gacaatatgg taattccaaa
     3721 aacggcacaa aataaagagg gtgcatataa atttatgaat tttttattag atgctaaaaa
     3781 taacaagcaa aatacagaat tcgtaggcta tgcaacgcca aacaaggctg ctcgacaatt
     3841 gttgcctaaa gagattaaag acgaccatcg tttttatccg actaagaaag agcaagaacg
     3901 ccttgaagtt tataaagatt taggacctga agttttaagt gaatacaatg aaaacttttt
     3961 gaatttcaaa atgtcattaa aataaaatca taaatagtag ttatttactg tatatttagt
     4021 tataataacg ggataagtac tataaggagt tgtaacgaat gactggagaa caatttactc
     4081 aaattaaacg tccagtaagt agattaactg aaaaagttct aggttggtta tgttgggtga
     4141 tgttattagt gcttactgtc attacgatgt ttatagcact tgtttcattc agtaataaca
     4201 catcgatagc taatcttgaa aatacattaa acaataatgc atttatccag caattattag
     4261 ctggaaatgg ctataataca acacaatttg taatatggtt acaaaatggt atatgggcta
     4321 ttatcgttta ctttattgtt tgtttgttga tttcattttt agctctcata tctatgaata
     4381 taagaatctt gtcaggtttc ttattcttaa tatcagcaat tgtaacgatt cctttagttt
     4441 tacttatcgt tactttaatc attccgatat tattctttat tattgcgatg atgctattta
     4501 taagaaaaga taaagttgaa atggttgcgc cacaatatta tgaagagtat aacggaccga
     4561 tttatgatta tcgtgaacct gtgtatgagc gcccccaacc gaaagatgat tattatgatg
     4621 tgcctaaata tgaaaaagaa ttggataaat caaatactgt atatgatcaa gaacaggaaa
     4681 gagataaata tgatcaattt cctaaacgtg cagttgaaag tgaatataat catgatgagc
     4741 gcactgagga agaaccatca gtattatcca gacaggctaa atacaaacaa aaaagtactg
     4801 aagaactagg tattgaagat gatggttatt atgcagaacc tgaagttgat ccaaaagaat
     4861 tgaaagcaca acaaaagcga gaaaaagctg aaattaaagc taagaaaaaa gaaaaacgta
     4921 aagcttataa ccaacgaatg aaagaacgta ggaaaaatca acctagcgca gttagtcaac
     4981 gtcgaatgaa ttttgaagag cgacgtcaaa tttacaacaa tgatatttct gaagaacgca
     5041 attcaagtga agttaaggac aaaaaagagc aagaataaat attgatgata cccagattag
     5101 taagtagagg tcttttgctt attaatctgg gtttttatat gaggttaatc atcgataacg
     5161 tttaattaaa gtgtttaggt gtcaaaattt taaatgacga tttctcatta ccatacacct
     5221 aaattatcat caatctgaat tcagatgttt attataaaaa ttagatgaaa aatatgttaa
     5281 tatacaagta atttaatgtg cgtatatcta taggctcgta gtatacttat taaaatatta
     5341 aacaaagaaa ggattttaag atgaatagaa aacctgaatt aatcatggct tggattgcaa
     5401 atagtattag tattatttat ttattagtta tggggctatc ctatttttct ttaaaaagtg
     5461 gtaatgcatc acaacgtgaa gaattagcga agcaattatc tcagaacggt ggcaatgttt
     5521 ctttagatat gcttcagaca acaatgggtg cattagcaat tattttatta atttcaacac
     5581 tttatggtat atttgcgaca atttgtatta aagggcgtag aaaattatcg attatacttt
     5641 ttgttatcgc gataattgta agtttgatgg ctcttaattt aattgcaatt gtcttatggg
     5701 ttatcgtgat gattatgttg atttctaaaa aagaatcaaa agaaactaca cataaggacg
     5761 atgagtatat
//
LOCUS       DAOBBH010000150         5751 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.150, whole genome shotgun sequence.
ACCESSION   DAOBBH010000150 DAOBBH010000000
VERSION     DAOBBH010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.150"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            176..2881
                     /gene="acnA"
                     /locus_tag="P6L01_002076"
     CDS             176..2881
                     /gene="acnA"
                     /locus_tag="P6L01_002076"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HDP3031126.1"
                     /translation="MAANFKEQSKKHFDLNGQSYTYYDLKAVEEQGITKVSNLPYSIR
                     VLLESLLRQEDDFVITDDHIKALSQFGKDGNEGEVPFKPSRVILQDFTGVPAVVDLAS
                     LRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFL
                     NWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMI
                     NGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELR
                     KKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSD
                     EHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKS
                     SFENSVTAPAGNQGHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPY
                     VMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRDAGLQPYLDDLGFNLVGYG
                     CTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAY
                     ALAGTVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYN
                     NNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVT
                     TDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLA
                     PGTEGGFTTYWPTNEVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLG
                     VKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYV
                     KVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ"
     gene            3061..3528
                     /locus_tag="P6L01_002077"
     CDS             3061..3528
                     /locus_tag="P6L01_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDP3031127.1"
                     /translation="MIYSITEIEARYAETDKMGVIYHGNYATWFEVARLDYISKLGFS
                     YADMEKQGIISPVTDLNVNYKKSIFYPEKVKVKTWVEKYSRLRSVYKYEIFNEKGELA
                     TTGSTELICIKEDTFKPIRLDRYFPDWHEAYSKVQTLNNEGKTVEIMDGIDSL"
     gene            complement(3735..4031)
                     /locus_tag="P6L01_002078"
     CDS             complement(3735..4031)
                     /locus_tag="P6L01_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HDP3031128.1"
                     /translation="MQIELTDAAVTWFKNELELPENNKVLVFFVRYGGEFQLKQGFSP
                     AFTVEPKEDVDIGYEQQYDDLNVVVAEKDLWYFEDDHIIVNVVDHEDEISYSTK"
     gene            complement(4407..5015)
                     /gene="plsY"
                     /locus_tag="P6L01_002079"
     CDS             complement(4407..5015)
                     /gene="plsY"
                     /locus_tag="P6L01_002079"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDP3031129.1"
                     /translation="MMIIVMLLLSYLIGAFPSGFVIGKLFFKKDIRQFGSGNTGATNS
                     FRVLGRPAGFLVTFLDIFKGFITVFFPLWLPVHADGPISTFFTNGLIVGLFAILGHVY
                     PVYLKFQGGKAVATSAGVVLGVNPILLLILAIIFFIVLKIFKYVSLASIVAAICCVIG
                     SLIIQDYILLVVSFLVSIILIIRHRSNIARIFRGEEPKIKWM"
     gene            5221..>5751
                     /locus_tag="P6L01_002080"
     CDS             5221..>5751
                     /locus_tag="P6L01_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499879.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDP3031130.1"
                     /translation="MNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEI
                     VDNSVDEVLNGYGNEIDVTINKDGSISIEDNGRGMPTGIHKSGKPTVEVIFTVLHAGG
                     KFGQGGYKTSGGLHGVGASVVNALSEWLEVEIHRDGSIYHQSFKNGGSPSSGLVKKGK
                     TKKTGTKVTFKPDDTIF"
BASE COUNT         2002 a          872 c         1076 g         1801 t
ORIGIN      
        1 aaaaaatact tattacacct tacctgtatg ataagttttg cttatatact ctgattaaaa
       61 agtcaaaacc taaattaagc gttttctaag catgatgagt tgtttatcaa taaatataat
      121 aatacaattt aatcaaggca tataaatata aaaatgtatc aagggggatc attaaatggc
      181 tgcaaatttt aaagagcaat caaaaaaaca ttttgacttg aatggccaaa gttatactta
      241 ctatgattta aaagctgtag aagagcaagg tattactaaa gtttccaatt taccttattc
      301 aattcgtgtt ttgttagaat ctttacttcg tcaagaagat gattttgtaa ttacagacga
      361 tcatattaaa gctttaagtc agtttggaaa agatggaaat gaaggcgagg taccatttaa
      421 accttctcgt gttattttac aagatttcac aggtgtacca gccgtagttg atttagcttc
      481 tttacgtaaa gcaatggatg acgttggggg agatattact aaaattaatc cagaagtacc
      541 ggtggattta gttattgacc actcagttca agtggatagc tatgcaaatc cagaagctct
      601 tgaacgtaat atgaaattag aatttgaacg taactatgaa cgttatcagt ttttaaattg
      661 ggcaacgaaa gcatttgata attacaatgc agttcctcct gcaactggaa tagttcacca
      721 agttaactta gaatatttag caagtgttgt acatgttcgt gatgtagatg gtgaaaaaac
      781 tgcatttcca gatacattag ttggtactga ttcacataca acaatgataa atggtattgg
      841 cgtactagga tggggtgttg gtggtattga agctgaagct ggaatgcttg gacaaccttc
      901 ttatttccca attccagagg ttattggtgt acgactagta aattcattac cacaaggcgc
      961 aacagcaact gatttagcgt taagagtaac tcaagagcta cgtaaaaaag gtgttgttgg
     1021 taaatttgtg gagttctttg gtccaggtgt acaacattta ccactagcag accgtgctac
     1081 aattgcaaac atggcaccag agtatggagc aacttgcgga ttcttcccag ttgatgatga
     1141 atctcttaaa tatatgaagt taactggtag atcagacgaa catatcgcgc tagtaaaaga
     1201 atatttgaaa caaaaccata tgttctttga tgttgagaaa gaagatccta attatacaga
     1261 tgttatcgaa ttggatttat caacagttga agcatcgctt tcaggaccaa aacgtcctca
     1321 agatttaatt ttcttaagtg atatgaaatc atcatttgaa aattctgtaa cagctccagc
     1381 aggcaaccaa ggacacggtt tagataaaag tgaatttgat aagaaagctg aaattaactt
     1441 taaagatgga tcaaaagcta caatgaaaac aggtgatatt gcaatagcag caattacatc
     1501 atgtacaaat acatctaacc cttatgtaat gttaggtgca ggtttagttg ctaaaaaagc
     1561 agttgaaaaa ggcttgaaag ttcctgaata cgttaaaact tctctagcac caggatcaaa
     1621 agttgttacc ggatatttaa gagatgctgg cttacaacct tatttagatg atttaggctt
     1681 caacttggtt ggttatggat gtacaacttg tatcggtaat tcaggtcctt tattaccaga
     1741 aattgaaaaa gcgattgctg atgaggacct attagtgaca tctgtattat ctggtaaccg
     1801 taactttgaa ggtcgtatcc atcctcttgt taaagccaat tacctagctt caccacagtt
     1861 agttgttgct tatgcattag ctggaacggt tgatattgat ttacagaatg aacctattgg
     1921 taaaggtaat gacggtgaag atgtatattt gaaagatatt tggccatcaa ttaaagaagt
     1981 ttcagatacc gttgatagtg ttgtaacacc tgaattattt attgaagaat ataataacgt
     2041 atacaataac aacgaattat ggaatgagat tgatgtaact gatcaacctc tatatgactt
     2101 tgatcctaat tcaacataca ttcaaaatcc atcattcttc caaggattat ctaaagaacc
     2161 gggtacgatt gttccattaa atggtttacg tgttatgggt aaattcggtg attctgtgac
     2221 aactgaccac atctctccag caggtgcaat tggtaaagat acgccagctg gtaaatattt
     2281 acaagatcat caagtgccta ttcgtgaatt taattcatat ggttcaagac gtggtaatca
     2341 cgaagtaatg gttcgaggta cgtttgctaa tatacgtatt aaaaaccaat tagcgccagg
     2401 tactgaaggt ggttttacaa cttattggcc aacaaatgaa gtaatgccta tctttgatgc
     2461 tgcaatgaaa tataaagaag atggtacagg tttagttgta ttagctggta acgattatgg
     2521 tatgggttca tctcgtgact gggcagcaaa aggtacaaac ttattaggtg ttaaaacagt
     2581 tattgcacaa agttatgaac gtatccatcg ttcaaattta gttatgatgg gtgtattacc
     2641 attagagttt aaaaaaggtg aatcagctga ttctcttggt ctagatggta cagaagaaat
     2701 ttctgttaat attgatgaaa atgttcaacc acatgactac gtcaaagtta ctgctaagaa
     2761 gcaagatggt gatttggtag aatttgacgc tatggttcgt tttgactcac ttgttgaaat
     2821 ggattactat cgtcacggtg gaattttaca aatggtttta agaaataaat tagcgcaata
     2881 aaaaatagat atcacagtaa aatttcaatc ggtatttgag gcactttgat agtgctcaaa
     2941 aatacttcta taaactttat gatataatag gtcggttcaa aacctttgtg ttcttggccg
     3001 gcctcttctt gtattttatg ttagaatgag atataaagtt aaactgaaag aaggcttaaa
     3061 atgatatata gtattacaga aatagaagcg cgttatgctg aaactgataa gatgggtgta
     3121 atttatcacg ggaattatgc aacttggttt gaagttgcgc ggttggatta tatatcgaag
     3181 ttaggtttta gttatgctga tatggaaaaa caaggaatca tttcacctgt gactgacctc
     3241 aatgtcaatt ataaaaagtc tattttttat ccagaaaaag ttaaagttaa aacttgggtt
     3301 gaaaaatatt cgagattacg ttcagtgtat aaatatgaga tttttaatga aaaaggtgaa
     3361 cttgcaacta caggttccac agaattgatt tgcattaaag aagatacttt taagcctata
     3421 cggttggatc gttatttccc agattggcat gaagcttata gtaaagtgca aacgctcaat
     3481 aatgaaggga aaacagtaga gataatggat ggtattgatt ctttataagt tactgtaaat
     3541 gtttggagag gcaatattaa caattaatct ataagaaata gccaatacgt taacattaat
     3601 gtaattagcg tgaagataag ctaatatata gcaaaaacga acttgttcat acaacatcag
     3661 atgtgctaaa ccaaagtaca acttccgagg ttcaggacac atcagaatgt gtaaacaagt
     3721 tcgttttatt ttagttattt tgtggaataa gaaatttcat cttcgtgatc aactacattt
     3781 acaataatgt ggtcatcttc aaagtaccac aaatcttttt ccgctacgac aacatttaaa
     3841 tcgtcatatt gttgttcata gccaatatca acatcttcct ttggttcaac tgtaaaagca
     3901 ggactaaatc cttgcttgag ttggaattcg ccaccatatc ttacaaaaaa cacgagcact
     3961 ttattatttt caggcaactc aagttcattt ttaaaccaag ttactgctgc atcagtaagt
     4021 tctatttgca taacaatgac ctcctatatt tattacagaa tgggagtggg ccaacttgta
     4081 ttatttgtgg aatttcttat cgaaattctc tgtgttggag cccaccccaa cctgctcatt
     4141 attgtaagct gaatttccat aagcttctat gttgggaccc ccgaccagaa ttgaaaaaac
     4201 ctagttataa gcgcatttcg ttcggttaac tacagctaat ataacattgt ggagcctatg
     4261 acattgattt atgtctcggt ctcaaatgtt cttgtaaaaa aactaagtat agttgaattt
     4321 taagttaaaa aaattgtttt tgcaaaaata aaataatcca tcaactgcaa aaaaatatgc
     4381 aatgatggac taatgtcaaa aaattgttac atccatttta ttttaggttc ttcgcctcta
     4441 aaaatccttg cgatattaga gcgatgtcta attatcaata tgattgaaac taagaaacta
     4501 acgactaata aaatatagtc ttgaatgata agcgagccaa tcacacagca aattgctgca
     4561 acgatacttg ctaaagaaac atatttaaaa atcttcaata caataaagaa gataattgca
     4621 agtattagta aaagtatcgg attgactccc aagacgacac ctgcactagt tgcaacagct
     4681 ttgccacctt ggaattttaa ataaacagga taaacgtgtc caagtatagc gaataagcca
     4741 acaattaaac catttgtaaa aaaagtacta atagggccat ctgcgtgaac tggtaaccat
     4801 aaagggaaga aaacagttat gaaccctttg aaaatatcta gaaatgttac caagaatcct
     4861 gcaggacgac ctaatactct aaagctatta gtagcgccag tattaccact accaaattgt
     4921 ctaatatctt ttttgaaaaa taattttcca attacgaatc cacttgggaa agcgccgata
     4981 agataactta gtagtaacat gacgattatc atcataaata ttacacatcc tttaatatct
     5041 taggactatt ttatcatatt catttaaatt acggctaaaa attttaaaaa cggggattaa
     5101 tatatggaat taagctatga aagttaattg atacttgcat tttacgctga tttatataag
     5161 aataactatt gtatagtttt aaaaacgaac gtacgtttgc aggaggcgaa atcattggca
     5221 atgaataaac aaaataatta ttcagatgat tcaatacagg ttttagaggg gttagaagca
     5281 gttcgtaaaa gacctggtat gtatattgga tcaactgata aacggggatt acatcatcta
     5341 gtatatgaaa ttgtcgataa ctccgtcgat gaagtattga atggttacgg taacgaaata
     5401 gatgtaacaa ttaataaaga tggtagtatt tctatagaag ataatggacg tggtatgcca
     5461 acaggtatac ataaatcagg taaaccgaca gtcgaagtta tctttactgt tttacatgca
     5521 ggaggtaaat ttggacaagg cggctataaa acttcaggtg gtcttcacgg tgttggtgct
     5581 tcagttgtaa atgcattgag tgaatggctt gaagttgaaa tccatcgaga tggtagtata
     5641 tatcatcaaa gttttaaaaa cggtggttcg ccatcttctg gtttagtgaa aaaaggtaaa
     5701 actaagaaaa caggtaccaa agtaacattt aaacctgatg acacaatttt t
//
LOCUS       DAOBBH010000151         5729 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.151, whole genome shotgun sequence.
ACCESSION   DAOBBH010000151 DAOBBH010000000
VERSION     DAOBBH010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5729
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.151"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            419..805
                     /locus_tag="P6L01_002081"
     CDS             419..805
                     /locus_tag="P6L01_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDP3031131.1"
                     /translation="MAIVNKVIIVEGKSDKKRVQQVIAEPVNIICTHGTMSIDKLDDM
                     IESLYDKQVFVLADSDDEGDRIRNWFKRYLSESEHIFIDKTYCQVANCPKQYLAHVLS
                     KHGFTCKKETPLLPNINNERLVLVNE"
     gene            798..1094
                     /locus_tag="P6L01_002082"
     CDS             798..1094
                     /locus_tag="P6L01_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDP3031132.1"
                     /translation="MNNSLDIKDVTTFYEEDKHLIFGYTPTCGTCKVSERMLDIANEI
                     LQLPLLKIDLNFYPQFCKDMQIMSTPILLLMNKDKEVKRIYAFKSVTDLLENLK"
     regulatory      1152..1255
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1344..2369
                     /locus_tag="P6L01_002083"
     CDS             1344..2369
                     /locus_tag="P6L01_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDP3031133.1"
                     /translation="MIELKEVVKEYRTKNKEVLAVDHVNLSIRAGSIYGVIGFSGAGK
                     STLIRMFNHLEAPTSGEVIIDGDHIGQLSKNGLRAKRQKVSMIFQHFNLLWSRTVLKN
                     IMFPLEIAGVPRRRAKQKALELVELVGLKGREKAYPSELSGGQKQRVGIARALANDPT
                     VLLCDEATSALDPQTTDEILDLLLKIREQQNLTIVLITHEMHVIRRICDEVAVMESGK
                     VIEHGPVTQVFENPQHTVTKRFVKEDLNDDFETSLTELEPLEKDAYIVRLVFAGSTTT
                     EPIVSSLSTAYDIKINILEANIKNTKNGTVGFLVLHIPYISSVDFGKFEKELIERQVK
                     MEVLRHG"
     gene            2362..3057
                     /locus_tag="P6L01_002084"
     CDS             2362..3057
                     /locus_tag="P6L01_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3031134.1"
                     /translation="MGKSFSEIINEMITMPNIQWPEVWTAIVETLYMTVVSTIFAFIL
                     GLILGVLLFLSAKGKSIGARLFYSIVSFIVNLFRAIPFIILILLLIPFTSLILGTISG
                     PTGALPALIIGAAPFYARLVEIAFKEIDKGVIEAAWSMGANTWIVIRKVLLPEAMPAL
                     VSGITVTAIALVGSTAVAGVIGAGGLGNLAYLTGFTRNQNDVILVSTVFILIIVFIIQ
                     FIGDWLTNKLDKR"
     gene            3074..3895
                     /locus_tag="P6L01_002085"
     CDS             3074..3895
                     /locus_tag="P6L01_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDP3031135.1"
                     /translation="MKKLFGLILVLTFAVVLAACGNGNKSGSDDKKITVGASPAPHAE
                     ILEKAKPLLEKKGYDLDIKTINDYTTPNKLLDKGEIDANYFQHTPYLNTEKKDKGYKI
                     VSAGDVHLEPMAVYSKKYKSLKELPKGATVYVSNNPAEQGRFLKFFVDAGLIKIKKGV
                     KIEDAKFSDITENKKDIKFNNKQSAEFLPKIYQNEDADAVIINSNFAIEQKLNPKKDS
                     IAVESAKDNPYANLIAVKEGHQDDKKIKALIEVLQSKDIQDFINEKYNGAVIPAK"
     gene            4249..4443
                     /locus_tag="P6L01_002086"
     CDS             4249..4443
                     /locus_tag="P6L01_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDP3031136.1"
                     /translation="MADESKFEQAKGNVKETVGNVTDNKNLENEGKEDKASGKAKEFV
                     ENAKEKATDFIDKVKGNKGE"
     gene            complement(4677..5528)
                     /locus_tag="P6L01_002087"
     CDS             complement(4677..5528)
                     /locus_tag="P6L01_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDP3031137.1"
                     /translation="MQQFKWINILKGFAMGTSDLVPGVSGGTIALLLGIYNQFIASIS
                     GIFSRRFWPSFTFLIPIIIGMLLAMGSLSNLFNYLLSQHHIPTMFFFGGLIIGIVPYL
                     LKISNYKTSFTTKHYMMVIAGIAILIVITLMNNGDKHAGETLTLSTGLIIKYFIAGMC
                     ASSAMLLPGISGSFMLLVFGVYGTVMLAISEVVKLNFTGLPILLAVGFGVLAGFIISS
                     KIIQYFLTHHKLMTFALIIGFVVGSLFAVFPGLPTNIVMWFVSLVVFIIGFIVSLTLG
                     RITAENE"
BASE COUNT         2095 a          801 c         1014 g         1819 t
ORIGIN      
        1 ttcataagcc acagctaatg tgtatttaaa aataggaata catgagtaaa actcatgcat
       61 aagaaatact aatttctaaa gaaaaagtat ttctttatgt tgggcccccc caacttgcac
      121 attattgtaa gctgacttac catcagcttc tgtgttgggg ccccgctata attgaaaagt
      181 ttgttatagg tgtattttct tttggttaac tattgttaat ataacattgt agattttagg
      241 atgttgattt ttgacttagc ctttgtctgc ttatgttaaa tgttccagct agtcaaacat
      301 tgagtttgta attattatat taagctagtt taaatttttt gtgatttatg ttttattcat
      361 accaattttg tgaaatgata cagtgacaga tgtttattga ttttaaaggg tgatgatgat
      421 ggctattgta aataaagtga taattgttga aggaaaatct gataaaaaaa gggtgcaaca
      481 ggttattgca gaaccagtca atattatttg tactcatgga acaatgagta tagataagct
      541 tgatgatatg atagaatcac tgtatgataa acaagttttt gtattagccg attctgatga
      601 cgaaggagat cgaattagaa attggtttaa acgttatttg agtgaaagtg aacatatatt
      661 tattgataaa acttactgtc aagttgcgaa ttgccccaaa caatatttgg cgcatgtact
      721 ttcaaaacat ggctttactt gtaagaaaga aacacctctt ttaccgaata taaataatga
      781 aaggttagtt ttagtaaatg aataattcat tagacatcaa agatgtaact acattttatg
      841 aggaagacaa acatttaatc tttggttata caccaacgtg tggtacttgt aaggtttcag
      901 aaagaatgtt agacattgct aatgaaatat tgcagttacc attattgaaa atagatttaa
      961 acttttatcc tcagttttgt aaagatatgc aaatcatgtc tacgccgatt ttattgttga
     1021 tgaataaaga taaagaagta aaacgaattt atgcatttaa atcggtgact gatttgttag
     1081 aaaatttaaa atagttattg acgaaatttt actaaggtgt taggattaat acaaattaaa
     1141 tgatgcgtaa actcttatcg agagtggtgg agggatgtgc cctacgaagc ccggcaaccg
     1201 tcttatatag aaatggtgcc aattcacata aagttttaac ttttgaagat gagagaaaca
     1261 atactactat tgctttctca atttttctat cgatattgag aaagcatttt ttattttatt
     1321 aagcaacaca gggaggaatc aacgtgattg aattaaaaga agttgttaaa gaatatcgga
     1381 ctaaaaataa agaagtcctt gctgtagatc acgttaattt atcgattcga gcaggatcga
     1441 tttatggcgt cattggtttt tctggagcag gaaaaagtac tttgattcga atgtttaatc
     1501 atttagaagc gcctacatca ggtgaagtta ttatagatgg agaccatata ggtcaattgt
     1561 ccaaaaatgg attaagagca aaaagacaaa aagtaagtat gatcttccaa cattttaatt
     1621 tgttatggtc aaggactgtg ttaaaaaata ttatgtttcc gcttgaaatt gcaggtgtcc
     1681 ctagaaggag agctaagcaa aaagcattag aacttgtcga actcgtcggt ttaaaaggta
     1741 gagaaaaggc ttatccatca gagttatcag gtggacaaaa gcaacgtgtt gggattgcac
     1801 gagcgttagc taatgatcca acggtcttgc tttgtgatga ggcaacaagt gcacttgatc
     1861 cgcaaacaac agatgaaatt ttagatctac tactaaaaat tagagaacaa caaaatttaa
     1921 caattgtact aattacgcat gaaatgcatg tcattcgtcg tatttgtgat gaagttgcag
     1981 ttatggaaag tggtaaagtg atagaacatg gaccggtgac acaggttttt gaaaatccgc
     2041 aacacactgt gacaaaacga tttgtgaaag aagatttaaa tgatgatttc gaaacatctt
     2101 taacagaatt agagccatta gaaaaagatg cttatatcgt tagattagtt ttcgctggtt
     2161 caacaacaac cgagcctatt gtatcgagtc tatcaactgc ctatgatatt aaaattaata
     2221 ttttagaagc aaatattaaa aatacaaaaa atggaacagt cggcttttta gttctgcata
     2281 ttccatatat ttcaagtgta gatttcggaa aattcgaaaa agagttaatt gagcgacaag
     2341 ttaaaatgga ggtgttaaga catgggtaaa tcatttagtg aaattataaa tgaaatgatt
     2401 acaatgccta atattcagtg gccagaagtt tggactgcaa tagtcgaaac actatacatg
     2461 acagtcgtct caactatatt tgcatttata ctcggtctta ttttaggtgt gttattattc
     2521 ttgtctgcta aaggtaagtc tatcggtgca aggttatttt attctatcgt ttctttcatt
     2581 gttaacttat ttagagcgat accatttatt attttaattt tattattaat tccatttaca
     2641 agtttgatac ttggaacgat aagtggtccg acaggtgcgt taccagcctt gattattggc
     2701 gcagcaccgt tttatgcaag gctcgtagaa attgctttta aagaaattga taaaggtgtc
     2761 atcgaagcgg cctggtcaat gggtgccaat acttggatag taattcgtaa agtcctctta
     2821 cctgaagcca tgccagcgct agtgtctggc attacagtta cagcaatcgc tttagttggt
     2881 tcaacagcag ttgcaggtgt aattggtgcc ggtggtttag gaaatttagc atacttaaca
     2941 ggtttcactc gaaatcaaaa tgatgtcatt ttagtatcaa ctgtttttat tttaattatt
     3001 gtatttataa tccaattcat tggggattgg cttacaaata aacttgataa acgataaatt
     3061 gggggttcat tttatgaaaa aattatttgg tcttatttta gtattaacat ttgcagttgt
     3121 attagcagct tgcggtaatg gaaacaaaag tggcagtgac gataagaaaa taacagtagg
     3181 tgcttcacca gcaccacatg ctgaaatttt agaaaaagca aaaccattat tagagaaaaa
     3241 aggttatgac ctagatatta aaacaattaa cgattacact acgcctaata aattactaga
     3301 taaaggtgaa attgacgcaa actatttcca acatacacca tatttaaaca cagagaaaaa
     3361 ggataaaggt tacaaaatcg taagtgccgg tgatgttcac ttagaaccta tggctgtata
     3421 ctctaaaaag tataaaagtt taaaagaatt accaaaaggt gcgacagttt atgtgtctaa
     3481 taatccagct gaacaaggac gcttcttaaa attcttcgtt gatgcaggtt taattaagat
     3541 caaaaaaggc gtaaaaattg aagatgctaa gtttagtgat attacagaga ataaaaaaga
     3601 tattaagttt aataataaac aatcagcaga attcttacct aaaatttatc aaaatgaaga
     3661 cgctgatgct gttatcatta attcaaactt tgcaatcgaa caaaaactaa atcctaaaaa
     3721 agattctatt gctgtagaaa gtgcgaaaga taatccttat gcaaacttaa ttgctgttaa
     3781 agaaggacat caagatgata agaaaatcaa agcattaatt gaagtattac aatctaaaga
     3841 tattcaagac ttcattaatg aaaaatacaa cggtgcagtt attcctgcta aataaatcat
     3901 attaatggct cagtaacgtg atgttgctgg gctttttaat ttaaacaggt atttatatga
     3961 tatttaggaa tgagatgcta ttacggataa agtaaaatcc tatatgcatg cgtgctactc
     4021 atttattctt atttttaatt tcacatggat agtgactact acatcataaa tcattagtga
     4081 ttctttatta tttcagtccc actccctcaa gttaatgatg aatctatttc gctagttata
     4141 aaatgacgtc aaatcaatat caaaaattat ttaagtaaaa tgtttagata atttttcagt
     4201 gggtaagtat tatatataac atttaattat tccgaggagg catttattat ggcagacgaa
     4261 agtaaatttg aacaagcaaa aggtaatgtt aaagaaacag taggtaatgt tactgataat
     4321 aaaaatttag aaaacgaagg taaagaagat aaagcttctg gtaaagcgaa agaattcgtt
     4381 gaaaatgcaa aagaaaaagc aactgatttt attgataaag taaaaggtaa caaaggcgag
     4441 taattttact tttttattcc taatacactg cacatgccac ttagtcggta taaccatcgg
     4501 ctaggtggtt tttgtattaa aaaagtggat accaaaattt attaataatt attttaatgt
     4561 tagaaaaaaa ctaaataaaa actcgctaat gatatccaat aatatgtata caaaacgaga
     4621 catatattgc atatgattaa cgagatactg aatatatttt atcaccccta aaatgattat
     4681 tcattttcag cggtaattcg acctaaagtc aaacttacaa taaaaccgat gataaatact
     4741 actaatgaaa cgaaccacat cacgatatta gttggtaaac ctggaaatac tgcaaagagg
     4801 gagccaacaa caaaaccaat gattaatgca aaagtcatta gtttatgatg tgttaggaaa
     4861 tactggataa ttttgcttga aataatgaat ccagcaagca ccccaaatcc gactgcaagt
     4921 agtataggaa gaccggtaaa gttaagttta acaacttcag atattgctag catgaccgta
     4981 ccatagacgc caaatactaa taacataaat gatcctgaaa tacctgggag taacatagcg
     5041 ctagatgcac acatacctgc aataaagtat ttaataataa gaccagttga tagagtaagc
     5101 gtttctccag catgtttatc accattattc attaatgtaa taacaattaa gatagcgata
     5161 ccagctataa ccatcatgta atgtttagtt gtaaatgacg ttttatagtt agaaattttc
     5221 aataaatatg gaacgatacc aatgattaat ccaccaaaga aaaacatagt tggaatatgg
     5281 tgttggctta ataaataatt aaaaagatta cttagtgatc ccattgccag taacattcca
     5341 attataatgg ggattaaaaa tgtaaaactt ggccaaaaac gtcgtgagaa tatgccgcta
     5401 attgaagcga taaattgatt gtaaatacct aacaataatg cgatagtccc accgctaaca
     5461 ccaggtacca agtcactcgt tcccatagca aaacctttta gaatattaat ccatttaaat
     5521 tgttgcatga ataactcctt tcaaacgatt ggaataaaat cataaatagc atcataccat
     5581 attacaaatg tcctagtgaa atgataacat attttaaatt cataaaatcc attgagaaat
     5641 tatgtgcact tattatcatt tatattttta aagagagcgg attagagttg tactttagag
     5701 ttattaaaaa taataaaaag ggtttaagt
//
LOCUS       DAOBBH010000152         5667 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.152, whole genome shotgun sequence.
ACCESSION   DAOBBH010000152 DAOBBH010000000
VERSION     DAOBBH010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.152"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(34..2076)
                     /locus_tag="P6L01_002088"
     CDS             complement(34..2076)
                     /locus_tag="P6L01_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDP3031138.1"
                     /translation="MEIFETILIFIAVVILSSFVHTFIPKVPLAFIQIFLGMLLFITP
                     IPVQFNFDSELFMVTMIAPLLFVEGVNVSRVHLRKYIKPVMMMALGLVITTVIGVGLF
                     IHWIWPDLPIGAAFAIAAILCPTDAVAVQAITKGKVLPKGAMTILEGESLLNDAAGII
                     SFKIAVGVLVTGAFSLVDAVQLFLIASIGGAVVGLLIGMALVRFRLTLMRRGYENINM
                     FTIIQLLTPFVTYLIAELFHASGIIAAVVAGLVHGFERDRIMQVRTQLQMSYNHTWNI
                     LGYVLNGFVFSILGFLVPEVIIKIIKTEPHNLIFLIGITIVVALAVYLFRFVWVYVLY
                     PYFYLAISPFQKMMTKNDDDNPTTEKPPKRSLYALIMTLCGVHGTISLAIALTLPYFL
                     AGHHAFTYRNDLLFIASGMVIISLVVAQVLLPLLTKPAPKTVIGNMSFKVARIYILEQ
                     VIDYLNQKSTFETSFKYGNVIKEYHDKLAFLKTVEKDDENSKELERLQKIAFNVETKT
                     LESLVDEGQITNSVLENYMRYAERTQVYRQASLIRRMIVLLRGALLKRRVQTRVNSAS
                     SLSVTDNLMELNKINKLVHYNVVSRLSKETTKDNTLEIGMVCDGYLMRIENLTPSNFF
                     NSASEDTITKIKLNALREQRRILRELIDTDEVSEGTALKLREAINYDEMVIVDSMT"
     gene            complement(2416..2853)
                     /gene="mnhG2"
                     /locus_tag="P6L01_002089"
     CDS             complement(2416..2853)
                     /gene="mnhG2"
                     /locus_tag="P6L01_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit G"
                     /protein_id="HDP3031139.1"
                     /translation="MEITKEIFSLIAAVMLLLGSFIALISAIGIVKFQDVFLRSHAAT
                     KSSTLSVLLTLIGVLIYFIVNTGFFSVRLLLSLVFINLTSPVGMHLVARAAYRNGAYM
                     YRKNDAHTHASILLSSNEQNSTEALQLRAKKREEHRKKWYQND"
     gene            complement(2828..3130)
                     /gene="mnhF2"
                     /locus_tag="P6L01_002090"
     CDS             complement(2828..3130)
                     /gene="mnhF2"
                     /locus_tag="P6L01_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit F"
                     /protein_id="HDP3031140.1"
                     /translation="MIQTITHIMIISSLIIFGIALIICLFRLIKGPTTADRVVTFDTT
                     SAVVMSIVGVLSVLMGTVSFLDSIMLIAIISFVSSVSISRFIGGGHVFNGNNKRNL"
     gene            complement(3127..3609)
                     /gene="mnhE2"
                     /locus_tag="P6L01_002091"
     CDS             complement(3127..3609)
                     /gene="mnhE2"
                     /locus_tag="P6L01_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HDP3031141.1"
                     /translation="MNQIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYILHR
                     FFSDDFYVRKIWVAIKFLGVYLYQLITSSISTINYILFKTKDMNPGLLSYETRLTSDW
                     AITFLTILIIITPGSTVIRISQDSKKFFIHSIDVSEKEKDSLLRSIKHYEDLILEVSR
                     "
     gene            complement(3610..5106)
                     /gene="mnhD2"
                     /locus_tag="P6L01_002092"
     CDS             complement(3610..5106)
                     /gene="mnhD2"
                     /locus_tag="P6L01_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit D"
                     /protein_id="HDP3031142.1"
                     /translation="MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTII
                     SLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGE
                     HKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
                     IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFS
                     AKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLA
                     TMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDI
                     VVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGK
                     VLIFQGALQNGNYIGLALMIITSLIAMYSLFRILFYMYFGDKDGEEVNFKKIPLYRKR
                     ILSILVVVVIAIGIAAPVVLNVTSDATELNTSDQLYQKLVNPHLKGED"
     gene            complement(5096..5440)
                     /gene="mnhC2"
                     /locus_tag="P6L01_002093"
     CDS             complement(5096..5440)
                     /gene="mnhC2"
                     /locus_tag="P6L01_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit C"
                     /protein_id="HDP3031143.1"
                     /translation="MNLILLLVIGFLVFIGTYMILSINLIRIVIGISIYTHAGNLIIM
                     SMGTYGSSRSEPLITGGNQLFVDPLLQAIVLTAIVIGFGMTAFLLVLVYRTYKVTKED
                     EIEGLRGEDDAK"
     gene            complement(5437..>5667)
                     /gene="mnhB2"
                     /locus_tag="P6L01_002094"
     CDS             complement(5437..>5667)
                     /gene="mnhB2"
                     /locus_tag="P6L01_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit B"
                     /protein_id="HDP3031144.1"
                     /translation="IDFRILMIIGALVSSITAIIPMFFGKPFLSQYETTWILPILGQI
                     HVSTITLFELGILFSVVGVIVTVMLSLSGGRS"
BASE COUNT         2069 a         1015 c          774 g         1809 t
ORIGIN      
        1 tttttcatca atccctttta gcataattag gaactacgtc atactatcta caataaccat
       61 ttcatcataa ttgatggctt ctcttagttt taacgctgta ccttctgata cttcatctgt
      121 atcaatcaac tcacgtaaaa tgcgacgttg ttctctcaat gcatttaatt taattttagt
      181 aatcgtatct tcacttgctg agttgaagaa atttgatggt gttaagtttt caattcgcat
      241 taaataaccg tcacaaacca ttccaatttc aagtgtatta tcttttgttg tttccttaga
      301 caaacgacta accacattat aatggactaa tttattaatt ttatttaatt ccattaagtt
      361 atccgtaaca ctaagtgaag atgcggagtt cactctcgtt tgtactcttc gttttaataa
      421 agcacctcgt aataatacaa tcattcttct tattaatgat gcttgtctat atacctgtgt
      481 tctttcagca taacgcatat agttttcaag tacactattc gttatttgtc cttcatctac
      541 taaagactct aatgtttttg tttctacatt aaaagcaatt ttttgtagac gttctaattc
      601 tttagagttt tcatcatctt tctctacagt ttttaaaaat gctaatttat catgatattc
      661 tttaatcacg ttaccatatt taaaacttgt ttcgaaagta gatttttgat ttagataatc
      721 aataacttgt tctaatatat aaattctagc aactttaaac gacatattgc caattactgt
      781 tttaggtgca ggtttcgtta ataatggcaa taatacttgc gcaactacca aactaataat
      841 aaccatacca gatgcaataa ataataagtc gtttctatac gtaaaagcat gatgccctgc
      901 taaaaaataa ggtaacgtta atgcgattgc taaagaaatt gttccatgca caccacataa
      961 cgtcataatt aaagcgtata aacttcgctt tggtggtttc tcagtcgttg gattatcatc
     1021 atcattttta gtcatcattt tttggaatgg actgatggct aaataaaaat aaggatataa
     1081 gacataaacc caaacaaatc taaatagata gacagctaaa gcaacaacaa tagtgatgcc
     1141 tattaaaaag attaaattgt gcggttctgt tttgataatt ttaataataa cttcaggtac
     1201 taaaaatcct aatattgaaa aaacaaagcc atttaaaaca taacctagta tattccatgt
     1261 atgattgtaa ctcatttgca gttgtgtacg tacttgcata attctgtcac gttcgaaacc
     1321 atgtacaagt cctgcaacta ctgctgcaat gattcctgat gcgtgaaaca attcagcaat
     1381 taaatacgta acaaatggtg ttaacaattg aataattgta aacatattaa tgttttcata
     1441 tcctcgacgc atcaatgtta atcggaacct tactaatgcc atacctataa gtaaaccaac
     1501 cactgcgcca ccaattgatg caattaaaaa caactgaaca gcatcaacaa gtgaaaaagc
     1561 acctgtaact aatactccaa cagctatttt aaatgaaata ataccagcag catcattcaa
     1621 taatgactca ccttcaagaa ttgtcattgc tccttttggc aagacctttc ctttagtgat
     1681 tgcttgcact gctactgcat cagtaggaca aagaatggca gcaattgcaa atgctgctcc
     1741 aataggtaaa tctggccaaa tccaatgaat aaataaacct acacctatca cagtagtaat
     1801 gactaatcct aatgccatca tcatcactgg cttaatatat ttccttaaat ggactctaga
     1861 aacattaaca ccttctacaa ataacaaagg cgcaatcatt gttaccataa acaattcaga
     1921 atcaaaatta aattgaacag ggattggggt aataaatagt aacatgccca agaaaatttg
     1981 tataaatgct aggggtactt taggtatgaa agtatggaca aacgaactta gtatcacaac
     2041 agctataaat ataagaattg tttcaaatat ttccaaactt tcacctctct aaaaagtatt
     2101 gtttaattga aaattaagta tcacatctcg ttgtaattat actttagagg ataaattgag
     2161 ttagcgacca caaaagcact ttaatataga tatatgtcta cgattgcagt acttaaattt
     2221 gcaattattt aattttattt tatcactaat tgtttgtata aataaacaac ttgctttcac
     2281 ataacaacat taacttataa tacaaaaaat gagcacctta aaatcgacta accaatttca
     2341 aagtactctt ttaatgatta attttgaaaa cagattttca aaagcattgt tatgcttaac
     2401 aatttagcca acacttcaat cgttttgata ccatttctta cgatgctctt ctcgtttttt
     2461 agcacgtaat tgtaatgctt ctgtagagtt ttgttcattt gaacttaata atattgatgc
     2521 atgtgtgtga gcatcatttt ttcgatacat ataagcgccg ttgcgataag cagcgcgagc
     2581 gactaagtgc atgccgactg gtgaagttaa attaataaaa acaagtgaca gtaataaacg
     2641 cacactgaaa aatcctgtat tcacaataaa ataaattaat acaccgatta aagttaataa
     2701 cacggataaa gttgaacttt ttgtcgcagc gtgacttctt aagaaaacat cttggaattt
     2761 cacgatacct attgcactaa taagagcaat aaaactacct aacaacaaca tcacagcagc
     2821 aataagacta aagatttctt ttgttatttc cattaaacac atgcccccca ccaataaagc
     2881 gtgatattga aacagaactt acaaaagata taatggcaat gagcatgatt gaatctaaga
     2941 aagaaacggt gcccataagt acacttaaca cacccacaat tgacattacg acagcacttg
     3001 ttgtatcaaa tgtaacgaca cgatctgctg ttgtaggtcc cttgattaat ctaaataaac
     3061 agatgattaa tgcaattcca aaaataatga gtgaactaat aatcataata tgtgttattg
     3121 tttgtatcat cgcgacacct ccaatattaa gtcttcataa tgcttaatac ttcttaacaa
     3181 actatctttt tctttttctg acacgtcgat actatgaata aaaaactttt tagagtcttg
     3241 agaaattcgt attactgtag accctggagt tataataatt aaaattgtta aaaatgttat
     3301 tgcccaatca cttgttagtc ttgtttcata tgaaagtaat ccagggttca tatcttttgt
     3361 tttaaaaaga atataattaa tcgtgctaat gctagatgtt attaattgat ataaataaac
     3421 acctaaaaat ttaatagcta cccatatttt tctaacataa aaatcatcgc tgaaaaacct
     3481 gtgtaatata taaatgacaa ttaaaccaat tagatatcca gaaaagaaag tcgagaattt
     3541 aaaatgatct tcatcttgaa ataataccca taagaatgca atgataatat ttaaaactat
     3601 ttgattcatt tagtcctctc ctttcaaatg cggatttaca agtttttgat ataattgatc
     3661 actcgtgttc aactcagttg catcacttgt aacatttaac acaacaggtg cagcaattcc
     3721 gattgcgata accacaacta ctaaaatact taaaattctt tttcgatata gcgggatttt
     3781 cttaaaatta acttcctccc catctttatc tccaaaatac atataaaaaa gtatcctaaa
     3841 taaactgtac attgcaatta gactagtaat aatcattaac gctagtccaa tataattgcc
     3901 attttgcaat gcaccttgga aaataagtac tttccccgga aagccactaa atggaggcac
     3961 gccgccaata gcaaaaatca ttataataaa cgcaactcca aataaaggtt cttttttagc
     4021 taagccattc aaatattgat attgtcgata gcctgtaatg taaactaaac taccaataat
     4081 aaagaatagc aatgttttta caacaatgtc atttaccaaa taaaatattg caccattaat
     4141 acctgcaaac gtgtttgttc ctaaacctaa aatgataaat cctattgaga ttatgacttg
     4201 gtaagctgca atctttttaa tatctttata agcaatgaca cctatagcgc cgatgaccat
     4261 agttatagca gccatagttg ctagcaatgg atgtatgaga tcattatgtt gatcaaatag
     4321 taaagtgaag aatcgaatta atgcataggc ccctactttg gtcattaacg ctgcaaataa
     4381 tgctgcaagc tcagtattta acacagcgta ggctttgggt agccacataa aaaggaccag
     4441 cgctgctttc gcactaaatg cgactaagaa gattaatgaa atcattgtaa cagtgcgatt
     4501 atctcccatg tcattcaaac gcattgcaat atgtgaaaag tttaatgtac ctactgtttt
     4561 ataaagtaaa cctataccta ataagaatag ccatgaacca ataatattca agacaacata
     4621 aataattgca gcacgtaatt gttctacaga ttgtccaagt gtaatgagta caaatgacgc
     4681 tagtaacata atttcaaaca tgacgtataa attaaataaa tctgatgtta gaaaagagcc
     4741 tatcacgcca acacttaaaa ataatatgaa cgatggcaag tgataacgat ttgctttatg
     4801 ttcgccacgc ccaaatccgt atgccataat taaagtaatc acaaacgaag cggttgtaac
     4861 cataatcaaa cttaaagaat ctcctaaaaa ctgtatacca aagggcgctg accatcctcc
     4921 aaagtctagc gtaattggac ggtgacgctg aacataaatt aatagcatta atgaaataat
     4981 tgtggtgata gtcattgtac ctaagtataa atatttagaa atacgatcat tattttttaa
     5041 aaatacaagg attaaggcac aaaggaatgg taataacatt ggtaaaatca ataagttact
     5101 tagcatcatc ttcccccctt aggccttcaa tttcatcttc ttttgttact ttataagttc
     5161 tataaacaag tacaagtaaa aacgcagtca tcccaaaccc tataactatt gcagttagta
     5221 caatagcttg taacaaggga tcaacaaaca attggtttcc accagttatt aatggttctg
     5281 atctactaga accatacgtt cccatactca taataatgag attaccagca tgagtatata
     5341 ttgaaattcc gattacaata cgaattaaat tgattgataa aatcatatat gttcctataa
     5401 acactaaaaa tcctataact agtaataata ttaaattcat gatcgacctc cgctaagcga
     5461 caacatcact gtgacaataa caccaacaac tgagaataaa atacctaatt caaaaagtgt
     5521 tattgtactt acatgaattt gccctaaaat tggaagtatc caagttgttt catattgaga
     5581 caaaaatggt tttccaaaaa acataggtat tatcgcagta atagatgata ccaatgctcc
     5641 aataatcatt aaaattctaa aatcaat
//
LOCUS       DAOBBH010000153         5653 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.153, whole genome shotgun sequence.
ACCESSION   DAOBBH010000153 DAOBBH010000000
VERSION     DAOBBH010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.153"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..776
                     /locus_tag="P6L01_002095"
     CDS             <1..776
                     /locus_tag="P6L01_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDP3031145.1"
                     /translation="HKQIKNLEQHLNVKLFETYKNQIILTEDGHTFLPIAQSFIEQYE
                     SGIKHIQLKKTMFQSKLNVVVSSYIATFIMPKFLKSFFNEHPFIDVSLHVKNENIEKD
                     INNHTYDIGISRNQPKLREVHSEKVCEGKIVLIAPNKENNHLLTEASLFEKYKIISDN
                     HPEYWSSLKNNILNIYGKAQFLSINDVHTSIKLIEMNQGISFLPIYITTDSDYNISVI
                     NTKILQAPISFTYIYSKKESPEILVFIKSFKKYIANEQL"
     gene            complement(835..3444)
                     /gene="clpB"
                     /locus_tag="P6L01_002096"
     CDS             complement(835..3444)
                     /gene="clpB"
                     /locus_tag="P6L01_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDP3031146.1"
                     /translation="MDINKMTYAVQSALQQAVELSQQHKLQNIEIEAILSAALNESES
                     LYKSILERANIEVDQLNKAYEDKLNTYASVEGDNIQYGQYISQQANQLITKAESYMKE
                     YEDEYISMEHILRSAMDIDQTTKHYINNKVEVIKEIIKKVRGGNHVTSQNPEVNYEAL
                     AKYGRDLVEEVRQGKMDPVIGRDEEIRNTIRILSRKTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKKDVPESLLDKTVFELDLSALVAGAKYRGEFEERLKAVLKEVKESDGRIILFI
                     DEIHMLVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLNEYREYIEKDSALERRFQK
                     VAVSEPDVEDTISILRGLKERYEVYHGVRIQDRALVAAAELSDRYITDRFLPDKAIDL
                     VDQACATIRTEMGSNPTELDQVNRRVMQLEIEESALKNESDNASKQRLQELQEELANE
                     KEKQAALQSRVESEKEKIANLQEKRAQLDESRQALEDAQTNNNLEKAAELQYGTIPQL
                     EKELRELEDNFQDEQGEDTDRMIREVVTDEEIGDIVSQWTGIPVSKLVETEREKLLHL
                     SDILHKRVVGQDKAVDLVSDAVVRARAGIKDPNRPIGSFLFLGPTGVGKTELAKSLAA
                     SLFDSEKHMIRIDMSEYMEKHAVSRLIGAPPGYIGHDEGGQLTEAVRRNPYSVILLDE
                     VEKAHTDVFNVLLQILDEGRLTDSKGRSVDFKNTIIIMTSNIGSQVLLENVKETGEIT
                     ESTEKAVMTNLNAYFKPEILNRMDDIVLFKPLSIDDMSMIVDKILTQLNIRLLEQRIS
                     IEVSDDAKAWLGQEAYEPQYGARPLKRFVQRQIETPLARMMIKEGFPEGTTIKVNLNS
                     DNNLTFNVEKIHE"
     gene            complement(3647..5461)
                     /locus_tag="P6L01_002097"
     CDS             complement(3647..5461)
                     /locus_tag="P6L01_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDP3031147.1"
                     /translation="MNKTKGFTKYKKMRYMPGLDGLRAIAVLGIIIYHLNKQWLTGGF
                     LGVDTFFVISGYLITSLLLKEYDDTGIIKLKSFWIRRLKRLLPAVIVLLMVVGTATLL
                     LKSDNIIRVKHDIIAAIFYVSNWWYIAKDVNYFEQFSFMPLKHLWSLAIEEQFYIFFP
                     VILVTLLLTIKKRYKIGFIFWGVSIISLGLMMFIYSINGDHSRVYFGTDTRLQTLLLG
                     VILAFLWPPFKLKNDPPKVVKYVIDSIGSLSFIVLILLFFIINDETNWIYDGGFYLIS
                     ILTLFIIASVVHPSTWIAKIFSNPVLVFIGKRSYSLYLWHFAVISFVHSYYVDGQIPV
                     YVYFIDISLTIIFAELSYRFIETPFRKEGIKALNWRPSYIPQFIRMAIVVTLLIPFML
                     ILVGAFNKYGKDIIGEKANSFDTTIEDNYLMRIAPIDNIHIDGLVSEKKKESSDVYNN
                     IKPLLIGDSVMVDIGESFKSSVPKSRIDGKVGRQLYQTLPLVKANYSQYKKSSDQVVL
                     ELGTNGDFTVKQLDDLLNQFGKAKIYLVNTRVPRIYEANVNRLLADAAKRKSNVTLID
                     WNKRSQGHSEYFAPDGVHLEYKGVLALKDEILKALKKK"
BASE COUNT         1827 a         1036 c          726 g         2064 t
ORIGIN      
        1 tacataagca aattaaaaat ttagaacagc atctcaatgt gaaactattt gaaacttata
       61 aaaatcaaat tatcttgact gaagatggtc atacatttct tccaattgcg caaagtttta
      121 ttgaacaata tgaaagtggc atcaaacata tccaacttaa aaaaacgatg tttcaatcga
      181 aattaaatgt tgtcgtgtct tcatatatcg cgacattcat tatgcccaaa tttttaaaat
      241 ctttctttaa cgagcatccg tttatcgatg tttctctcca tgtcaaaaat gaaaatattg
      301 aaaaagatat taataatcat acgtatgaca tcgggattag cagaaatcaa cctaaactaa
      361 gagaagttca ttctgaaaaa gtatgcgaag gtaaaattgt attgattgct cccaataaag
      421 agaataatca tcttctaact gaggcatctt tatttgaaaa atacaaaata ataagtgata
      481 accatccaga atattggtca tcacttaaaa ataatatttt aaatatttat ggaaaagcac
      541 aatttctatc tatcaatgat gtgcatactt caattaaatt aatcgaaatg aatcaaggta
      601 tatcattcct gcctatttat ataacaacag atagtgatta caatatttcg gttattaaca
      661 cgaaaatttt acaagcgcct atttcattta catatatata cagcaaaaaa gaaagcccag
      721 aaatattggt ttttattaaa tcatttaaaa agtatattgc caatgaacaa ttataataaa
      781 tttcaaatct aaaaaaccaa gaatgcgatt aatcatcaca ttcttggttc aattttattc
      841 atgaattttt tcaacattaa acgttaagtt attgtctgaa tttaaattaa ctttaatcgt
      901 tgtaccttct gggaatccct ctttaatcat catacgtgct aatggtgttt caatttggcg
      961 ttgtacaaaa cgttttaatg gtcttgcacc gtattgaggt tcataagctt cttgacctag
     1021 ccaagcttta gcatcatcag aaacttcaat tgagattcgc tgttctaata atcttatatt
     1081 taattgcgtt aatattttgt ctacaatcat actcatgtca tcaatagata gaggtttaaa
     1141 taatacgatg tcatccatac gattcaaaat ttctggtttg aaatatgcat ttaaatttgt
     1201 cataacagct ttttctgttg attctgtaat ttcaccagtc tcttttacgt tttctaataa
     1261 aacttgagat ccaatattac ttgtcataat aataatagta tttttaaaat caacgctacg
     1321 tcctttagaa tcagttaaac ggccttcatc taaaatttgc aataatacat taaagacgtc
     1381 agtatgcgct ttttcaacct catctaataa gataactgag tatggattac gacgaaccgc
     1441 ttcagttaat tgaccccctt catcatgtcc aatatatcct ggaggtgccc ctatcaatct
     1501 tgatactgca tgtttttcca tatattcact catatcaata cgaatcatat gtttttcaga
     1561 atcaaataat gatgcagcta atgatttagc taattcagtt ttacctactc cagttggacc
     1621 taggaataag aaactaccaa taggtctgtt tggatcttta atacctgctc ttgctctaac
     1681 tactgcatct gaaaccaggt caaccgcttt atcttgacct acaacacgtt tatgcaagat
     1741 gtcacttaag tgaagtaatt tttcacgttc tgtttcaact aattttgaaa ctggtatgcc
     1801 tgtccattgg ctgacaatat cgccaatttc ttcgtctgtt acaacttcac gaatcattcg
     1861 atctgtatct tcaccttgct catcttggaa attatcctct aattctctaa gttctttttc
     1921 caattgagga attgttccat attgtagttc agcagctttt tctaaattgt tatttgtttg
     1981 tgcatcttcc aacgcttgtc tactttcatc tagttgcgca cgtttttctt gtaaatttgc
     2041 tattttttct ttttctgatt ctacacgaga ttgaagtgct gcttgtttct ctttttcatt
     2101 ggcaagctct tcttgtagtt cttgtaatct ctgtttgctc gcattgtcag attcattttt
     2161 aagtgcgctt tcttcaattt ctaattgcat gacacgtcta ttaacttgat ccaattcagt
     2221 tggatttgat cccatttccg tacgaattgt tgcacatgct tggtcaacta aatcaatcgc
     2281 tttatctggt aaaaaacgat cagtgatgta acgatcagac aattcagcgg cagcaactaa
     2341 ggctctatct tgaatacgca caccatgata cacttcatat cgttctttta aaccacgtaa
     2401 aattgaaatt gtatcttcaa catcaggctc actaactgct actttttgga aacgacgctc
     2461 taatgccgaa tctttttcaa tatattctcg atattcattt aaagttgttg caccaataca
     2521 atgtaactct cctcgtgcta acattggttt tagcatgttg cctgcatcca tggcaccatc
     2581 tgttttacca gcacctacaa gcatatggat ttcatcaata aataatataa ttctaccatc
     2641 agactcttta acttctttta ggactgcttt taatctctct tcaaattcac cacgatattt
     2701 agcgcccgct actaatgcgc ttaaatctaa ctcaaaaaca gttttatcta ataatgattc
     2761 tggcacatct ttcttaacta tacgttgcgc taatccttca acaattgcag ttttaccaac
     2821 acctggttca ccaatgagca cagggttgtt tttagtttta cgacttaaaa tacgaatcgt
     2881 atttcgaatt tcttcatctc ttcctataac aggatccatt ttaccttgtc taacttcttc
     2941 tactaagtcg cggccatatt tagctaatgc ttcgtaatta acttctggat tttgtgatgt
     3001 cacgtgattt ccccctctta cttttttaat aatttctttg ataacttcta ctttattatt
     3061 tatataatgt tttgttgttt gatcaatgtc cattgccgaa cgtaaaatat gctccattga
     3121 aatatattca tcttcatatt ctttcatgta tgattcagcc ttagttatca attggtttgc
     3181 ttgttggcta atatattgac catattgtat attgtcacct tctacagatg catacgtgtt
     3241 tagtttgtct tcataagctt tgtttaattg atctacctca atatttgctc gttctaaaat
     3301 acttttatat aagctttcac tttcatttaa ggcagcgctt aaaattgcct caatttctat
     3361 attttgtaat ttatgttgct gactcagttc aactgcttgt tgtaaagcac tttgaacagc
     3421 atatgtcatt ttatttatat ccatataatt tcacctctaa tttgtagagt tggtcaagtt
     3481 gcacttttga ctttgacttt ctttgacctt aattatatta tagtcccaat ataatcattt
     3541 atcaactctt ttacacttaa atttcctaat atttgtaaat atttttttga ataaaaaaat
     3601 ctagctaatg ttacgttaca cattaactag acttattaaa catatgttat ttctttttaa
     3661 gtgcttttaa tatttcatct tttaaagcta ggactccttt gtactctaaa tgtacaccgt
     3721 ctggtgcaaa atattcacta tgtccttgtg atcgcttatt ccaatcaatt aatgtgacat
     3781 tggactttcg tttcgccgcg tcagctaata atcgatttac atttgcctca taaattcttg
     3841 gaacacgtgt attaactaaa taaatcttgg cttttccaaa ttgattaagt aaatcgtcga
     3901 gctgtttgac agtaaagtcg ccatttgtac ctaattctaa tacgacttga tcagatgatt
     3961 ttttatattg tgaataattc gctttaacta aaggtaaggt ttgatacaat tgacgcccta
     4021 cttttccatc aattctagac ttaggaactg atgacttaaa tgactcaccg atatcaacca
     4081 ttactgaatc accgattaaa agaggtttaa tattattata tacgtcggaa gattcctttt
     4141 tcttctcact tactaagcca tcaatgtgaa tgttatcaat tggtgctatc cgcattaaat
     4201 aattatcttc aatagtggta tcaaagctat tcgctttttc tccaataatg tctttaccat
     4261 atttattgaa tgcacctact aaaatcaaca taaatggaat taacaaggtt actacaattg
     4321 ccattcttat aaattgtggt atataagaag gtcgccaatt taaagcttta ataccttctt
     4381 ttctaaatgg agtttctata aagcgatatg atagctctgc aaatataatt gttaaactta
     4441 tatctataaa gtacacatat acaggtatct gtccatctac atagtaacta tgtacgaaac
     4501 taattactgc aaaatgccat aaatataaac tataagacct tttcccgata aatactaaca
     4561 ctggatttga aaatatcttc gctatccatg tagatggatg aacgacactg gcaataataa
     4621 ataacgttaa tatggatatt aaatagaaac caccatcata tatccaattc gtctcatcat
     4681 taataatgaa aaataataat ataagtacta taaatgataa actacctatg ctatcaataa
     4741 catattttac aactttaggt ggatcatttt tcaatttaaa cggtggccat aaaaaagcta
     4801 aaataacacc cagtaacaat gtctgtaatc tagtatctgt accaaaatac actcgtgaat
     4861 gatccccatt aatactgtag ataaacatca ttaaccctaa agaaattatt gatactcccc
     4921 aaaaaataaa tcctattttg tatcgctttt taattgttaa caataatgta accaaaataa
     4981 ctgggaaaaa tatgtaaaac tgttcttcaa ttgctaaaga ccataaatgc tttaatggca
     5041 taaatgaaaa ttgctcaaaa taattaacat cttttgctat ataccaccag tttgatacat
     5101 aaaatatcgc agcaataata tcatgtttaa ccctaatgat attatctgat tttaataata
     5161 aggttgctgt ccctacaacc attaataaaa ctatgactgc tggtaataaa cgttttaaac
     5221 gacgtatcca aaagcttttc aatttaatga tacctgtgtc atcatactct ttgagtaata
     5281 agcttgtaat taaataacca gagatcacaa aaaatgtatc cacacctaaa aagccacctg
     5341 tcaaccattg cttgtttaag tggtaaataa taattcctag aacagcgatt gccctcaaac
     5401 catcgagccc tggcatatat ctcattttct tatactttgt aaaacccttt gttttgttca
     5461 tttttgcatt cttccctttt aaaactgttc ttcttagatg cttaattaaa tttagttatg
     5521 ctgtttaaaa gaatattgaa atgcatatgt atattattga attacgacat catcaaaatc
     5581 atattgacta aaatactgtt aaattaaaaa aattaccaat gatgattctt acttcgaaat
     5641 ccaatttgta atg
//
LOCUS       DAOBBH010000154         5618 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.154, whole genome shotgun sequence.
ACCESSION   DAOBBH010000154 DAOBBH010000000
VERSION     DAOBBH010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.154"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..393
                     /locus_tag="P6L01_002098"
     CDS             <1..393
                     /locus_tag="P6L01_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HDP3031148.1"
                     /translation="EMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRD
                     ILEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFT
                     ANIYYKSIIKITITVKVGTNLQTKTIYV"
     gene            490..636
                     /locus_tag="P6L01_002099"
     CDS             490..636
                     /locus_tag="P6L01_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031149.1"
                     /translation="MMLTAIYFSIFTFYISQYSLKLKTLYNIETDYNYKIVLLLKEGE
                     THES"
     gene            626..1150
                     /locus_tag="P6L01_002100"
     CDS             626..1150
                     /locus_tag="P6L01_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDP3031150.1"
                     /translation="MNHDKSPIILIGFMGTGKSTIGKYVADEQNLSFIDIDSYIEEKY
                     KLTIPEIFSKHGEQYFRNLEFTCLQECINTADIIATGGGIIESEEAFNFLKNQKNIIW
                     LDCNIDIIYSRINDDPHRPNANNKTIKQLNDLYCSRILRYNEIAFKKFDSHLLSISEI
                     YYELLNLIKASDQY"
     regulatory      1137..1234
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            1309..2400
                     /gene="gcvT"
                     /locus_tag="P6L01_002101"
     CDS             1309..2400
                     /gene="gcvT"
                     /locus_tag="P6L01_002101"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HDP3031151.1"
                     /translation="MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVR
                     YEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGGIIDDLV
                     IYKLADDNYLLVVNAANTEKDFNWILKHKEKFDVEVQNVSNQYGQLAIQGPKARDLIN
                     QLVDEDVTEMKMFEFKQGVKLFGANVILSQSGYTGEDGFEIYCNIDDTEKIWDGLLEY
                     NVMPCGLGARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSVLKDQ
                     KENGAPRRTVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAMIKRD
                     EFEMGRELLVQVRKRQLKAKIVKKNQIDK"
     gene            2420..3766
                     /gene="gcvPA"
                     /locus_tag="P6L01_002102"
     CDS             2420..3766
                     /gene="gcvPA"
                     /locus_tag="P6L01_002102"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HDP3031152.1"
                     /translation="MSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNI
                     AEGEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTP
                     YQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIVV
                     SKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDETAAVAVQYPNFY
                     GSIEDLEKIQSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQPFGIPAQFGG
                     PHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQA
                     LNALASSIAMSALGKQGIYDIAVQNIEHANYAKQQFIKKGFEVLDGTSFNEFVVKFDK
                     PIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND"
     gene            3759..5231
                     /gene="gcvPB"
                     /locus_tag="P6L01_002103"
     CDS             3759..5231
                     /gene="gcvPB"
                     /locus_tag="P6L01_002103"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HDP3031153.1"
                     /translation="MTSKSSPLIFERSREGRYAYSLPKSDIKTNSVESLLDDKFIRKN
                     KAEFPEVAELDLVRHYTELSNKNFGVDNGFYPLGSCTMKYNPKINEKVARIPGFSESH
                     PLQDEDQVQGSLEIIYSLQEELKEITGMDEVTLQPAAGAHGEWTALMIFKAYHENNGE
                     GHRDEVIVPDSAHGTNPASASFAGFKSVTVKSNERGEVDIDDLKRVVNENTAAIMLTN
                     PNTLGIFEKNIMEIREIVHNAGGLLYYDGANLNAIMDKVRPGDMGFDAVHLNLHKTFT
                     GPHGGGGPGSGPVGVVKELASYLPKPMVIKDGDKFKYDNDIKNSIGRVKPFYGNFGIY
                     LRAYTYIRTMGATGLKEVSEAAVLNANYIKARLSEHFEIPYKQYCKHEFVLSGVRQKE
                     FGVRTLDMAKRLLDFGVHPPTIYFPLNVEEGMMIEPTETESKETLDYFIDTLISIAEE
                     AKNDPDKVLEAPHTTVIDRLDEATAARKPILKFENLKQEK"
BASE COUNT         2058 a          715 c         1055 g         1790 t
ORIGIN      
        1 gaaatgttag tagcgatgat ggttataagt ataattttac taattgttcc agacttaatt
       61 agacttagca aaacttttct aattgaaagt agggaattaa caactgtaga tttcgaattt
      121 ttctcaagag atattctaga ggattttaaa ggtgtagata aaaacgatat tgaaattagg
      181 caacagcgta tcattttaca taaaggtgaa gaaatgatcg aatacaaatt aataaataat
      241 aaaattatta aagttgtaaa tgacagagga aatataacaa tgattaataa tgttacagca
      301 tttactgcaa atatctacta taaatctatt attaaaataa cgataacagt taaagtcggt
      361 acaaatctgc agactaaaac tatttatgta taacttacta gaatttaaat gagtactatt
      421 aaaaaggatg tgaaataatt tattaatttt gatttatata aagaaaaagg atatgtaact
      481 ccgttcttat tgatgctaac agcaatttat ttttccattt tcacttttta tatcagtcaa
      541 tatagtttaa aattaaagac attatataat attgaaactg actataacta taagattgtt
      601 ttgttattga aagaagggga aacgcatgaa tcatgataaa tcaccaataa ttttaattgg
      661 tttcatgggt accggtaaat ctacgattgg taaatacgtt gcagatgagc aaaatttatc
      721 atttattgat atagattcat atatcgaaga gaagtataag ttaacaatac cagaaatatt
      781 tagtaaacat ggtgaacaat atttcaggaa tttagagttc acatgtttgc aagaatgtat
      841 taacactgca gatataattg ctactggtgg tggtattatt gaaagtgaag aggcatttaa
      901 ttttttgaaa aatcaaaaaa acattatttg gttagattgt aatattgata ttatatatag
      961 tcgaatcaat gatgacccac atcgacctaa tgcaaataat aagacaatca agcagttaaa
     1021 tgacttgtat tgctcgcgga ttttaagata taatgaaatc gcattcaaga aatttgatag
     1081 tcatttgcta tcaatttcag aaatatatta tgaattgcta aatttaataa aagcgagtga
     1141 tcagtattag agagaataga gcgttaagac tctatcgccg aaggtgcaag taatttatta
     1201 cgaaactctc aggcaaaagg ataatactgt aacgcgttcc tgaattggtg atttataaac
     1261 agggtagcga ttgctatcct gtttttataa ttttaagggg gtatttcaat gtcaagtgat
     1321 ttaaaacaaa cacctttata tcaaaattat gttgatagag gtgcaaaaat tgtggaattc
     1381 ggaggatggg cgatgcctgt tcaattttca agtattaaag aggagcataa tgctgttcga
     1441 tacgaaattg gcctgtttga tgttagtcat atgggtgaaa ttgaagtaac aggtaaagat
     1501 gctagtcagt ttgtgcaata tttattatca aatgatactg ataatttaac tacttcaaaa
     1561 gcattatata ctgctttatg taatgaagaa ggcggtatta ttgatgattt agtaatatat
     1621 aaattagctg acgacaatta tttattagtt gttaatgctg ctaatactga aaaagatttt
     1681 aattggattt taaaacacaa agagaaattt gatgttgaag tacaaaatgt atcaaaccaa
     1741 tatggtcaat tagcaataca aggaccaaaa gctagagatt taattaatca attagttgat
     1801 gaagatgtaa ctgaaatgaa aatgtttgaa tttaaacagg gtgtcaaatt atttggagca
     1861 aacgtcattt tatctcagtc aggttacaca ggtgaagatg ggtttgaaat ttattgtaat
     1921 atcgatgata ctgaaaaaat ttgggatggt ttattagagt ataatgttat gccatgtggg
     1981 ttaggcgctc gtgatacatt aagattagag gctggattgc cattacatgg acaagattta
     2041 actgaatcaa ttacaccata tgaaggtggt atcgcttttg caagtaaacc attaattgat
     2101 gctgatttta ttggtaaatc tgtattaaaa gatcaaaaag aaaatggtgc accaagaaga
     2161 acagtgggat tagaattact tgaaaaagga attgcaagaa ctggttatga agttatggat
     2221 ttagatggaa atattattgg agaagtaact tcaggaacac agtctccatc atcaggaaaa
     2281 tcaattgcac ttgcaatgat aaaaagagat gagtttgaaa tgggtagaga gttgcttgtt
     2341 caagttcgta agcgtcaatt aaaagcgaaa attgttaaga aaaatcaaat tgataaataa
     2401 ttaaaaaggg gtgtgcattg tgagtcatcg ttatatacct ttaactgaaa aagacaagca
     2461 agaaatgtta caaacaattg gtgcaaaatc tataggagaa ttattcggtg atgtaccaag
     2521 tgacatttta ttaaatagag atttaaatat tgctgaaggc gaagcggaaa caacgttact
     2581 tagaagatta aatcgcattg caagcaagaa tatcactaaa gaaacgcata catcattttt
     2641 gggtgctggt gtttatgatc attacgcgcc atcagttgtg gatgctatga tttcaagatc
     2701 agaattttac acagcatata cgccatatca accggaaatt tctcaaggtg aattacaagc
     2761 catttttgag ttccaaactt taatttgtga attaacagat atggatgtag caaattcttc
     2821 tatgtatgat gggatgacta gctttgctga agcatgtatc ttagcattta gtcaaacaaa
     2881 gaaaaataaa attgtagtct caaaaggttt acattatcaa gcattacaag tacttcatac
     2941 ttatgctaag acgcgtaagg aattcgaagt agtggaaatc gatttagatg gcactgttac
     3001 agatttgaaa aaattagaag cagcagtgga tgatgaaaca gcagctgttg cagtacaata
     3061 tccaaacttt tacggttcaa ttgaagatct tgaaaagatt caaagcttta ttgaagataa
     3121 aaaagcatta tttatcgtat atgcaaatcc attagcattg ggattactta cacctccagg
     3181 ttcatttggt gcagatattg ttgtaggtga tactcagcca ttcggaattc ctgctcaatt
     3241 cggtgggcca cattgtggat attttgcaac gacgaaaaag ttaatgcgta aagtgccagg
     3301 tagattagtc ggtcaaacac aagatgatga aggtaatcgt ggctttgtat taacattaca
     3361 agcacgtgaa caacatattc gacgcgataa agcgacatct aatatttgtt ctaaccaagc
     3421 attaaatgct ttagcgtcat caattgcaat gtcagcactt ggtaaacaag gtatctatga
     3481 tattgcagtt caaaatatag aacatgctaa ctatgctaaa caacaattta ttaaaaaagg
     3541 ttttgaagta ttagatggta catctttcaa tgagtttgta gtcaaatttg acaaaccaat
     3601 tcaacaagtt aacgaggaat tagtgaagta caatattatc ggtggttttg atttaggtgt
     3661 tgtttcagac gactttaaaa atcacatgtt aatagctgta actgaattaa gaacaaaaga
     3721 tgaaatcgat acatttgtag aaaaggctgg tgagttaaat gactagtaaa tcaagtccat
     3781 taatttttga aagatctcgt gaaggcagat atgcatattc attaccaaaa agtgatatta
     3841 aaacaaattc tgttgagtca ttgttagatg ataaatttat tcgtaaaaac aaagcggagt
     3901 ttcctgaagt tgctgaacta gacttagtac gtcattatac tgaactttca aataaaaatt
     3961 tcggtgtaga caacggattt tatccattgg gttcatgtac gatgaagtat aatcctaaaa
     4021 ttaatgaaaa ggttgctaga ataccaggat tcagtgaatc acatccatta caagatgaag
     4081 accaagttca aggttcatta gaaattattt atagtttgca agaagaatta aaagaaatta
     4141 ctggtatgga tgaggtaaca ttacaaccag ctgctggtgc acatggtgaa tggactgcat
     4201 tgatgatatt taaagcttat catgagaata atggtgaagg tcatcgtgat gaagtcattg
     4261 tgccagattc tgcgcatggt acgaatccag cctcagcttc atttgcagga tttaaatcag
     4321 ttactgtaaa atcaaacgaa cgtggcgaag ttgatattga tgacttgaaa cgtgttgtaa
     4381 atgaaaatac agcagctatt atgttaacta atccaaatac tttaggtatt ttcgaaaaaa
     4441 acattatgga aatccgtgaa atcgtccata atgctggtgg tctattatat tatgatggtg
     4501 cgaatttaaa cgctattatg gacaaagttc gcccaggaga tatgggattt gatgctgttc
     4561 atttaaactt gcataaaaca tttactggtc cacatggtgg cggcggtcct ggttcaggtc
     4621 cagtcggtgt agtaaaagaa ctagcaagtt acttaccaaa gccaatggtt attaaagatg
     4681 gcgacaaatt taaatatgat aatgacatta aaaattctat cggacgtgta aaaccatttt
     4741 atggtaactt tggtatttac ttaagagctt atacgtatat tcgaactatg ggagcaactg
     4801 gacttaaaga ggtttcggaa gcagcggttc ttaatgcgaa ttatattaaa gcacgtttat
     4861 ctgaacactt tgaaatacct tataaacaat attgtaaaca tgagtttgtg ttaagtggtg
     4921 tgcgtcaaaa agaatttggt gtacgtactt tagacatggc taagcgatta ttagatttcg
     4981 gtgtacatcc accaacaata tacttcccat taaatgttga agaaggtatg atgattgaac
     5041 cgactgagac agagtctaaa gaaacacttg attattttat cgatacatta attagtattg
     5101 ctgaagaagc taaaaatgat cctgataaag tgctagaagc accacataca actgtgattg
     5161 atcgattaga cgaagctaca gctgctcgta aaccaatatt aaagtttgaa aatcttaaac
     5221 aggaaaaata aagtattaaa cacatattcc gagaattatt attctaactt tgtatgaaga
     5281 tttaaggata atggtttcaa aatcaattga aaaagacaat ttctatttga acaagaaaaa
     5341 taaaccgaag taataactct tagggtttgg tattattctt tcatagaaat tgtcttttca
     5401 ttttttagat tgcggtaatt gaatcgtatt gaaaatgagc tgaactttct ttattatgct
     5461 gaaactaagt ttaatgatca gtgaagctag agaaatatag taacaggctt tgttaaaaaa
     5521 ttaaaatctg agtattgtta ttgatgatca attaacactc agattttatt aaatcgtata
     5581 ttaaatttac aaaaactatt ttttagactt tatttttc
//
LOCUS       DAOBBH010000155         5579 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.155, whole genome shotgun sequence.
ACCESSION   DAOBBH010000155 DAOBBH010000000
VERSION     DAOBBH010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.155"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..1446
                     /locus_tag="P6L01_002104"
     CDS             <1..1446
                     /locus_tag="P6L01_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499879.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDP3031154.1"
                     /translation="FNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEHYHYEEGIK
                     EFVSYVNEGKEVLHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVRTKDGGTHE
                     VGFKTAMTRVFNDYARRINELKTKDKNLDGNDIREGLTAVVSVRIPEELLQFEGQTKS
                     KLGTSEARSAVDSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKAREDARSGK
                     KNKRKDTLLSGKLTPAQSKNTEKNELYLVEGDSAGGSAKLGRDRKFQAILPLRGKVIN
                     TEKARLEDIFKNEEINTIIHTIGAGVGTDFKIEDSNYNRVIIMTDADTDGAHIQVLLL
                     TFFFKYMKPLVQAGRVFIALPPLYKLEKGKGKTKRVEYAWTDEELNKLQKELGKGFTL
                     QRYKGLGEMNPEQLWETTMNPETRTLIRVQVEDEVRSSKRVTTLMGDKVQPRREWIEK
                     HVEFGMQEDQSILDNSEVQVLENDQFDEEEI"
     gene            1446..3848
                     /gene="parC"
                     /locus_tag="P6L01_002105"
     CDS             1446..3848
                     /gene="parC"
                     /locus_tag="P6L01_002105"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDP3031155.1"
                     /translation="MSEIIQDLSLEDVLGDRFGRYSKYIIQERALPDVRDGLKPVQRR
                     ILYAMYSSGNTHDKNFRKSAKTVGDVIGQYHPHGDSSVYEAMVRLSQDWKLRHVLIEM
                     HGNNGSIDNDPPAAMRYTEAKLSLLAEELLRDINKETVSFIPNYDDTTLEPMVLPSRF
                     PNLLVNGSTGISAGYATDIPPHNLAEVIQATLKYIDNPDITVNQLMKYIKGPDFPTGG
                     IIQGIDGIKKAYESGKGRIIVRSKVEEETLRNGRKQLIITEIPYEVNKSSLVKRIDEL
                     RADKKVDGIVEVRDETDRTGLRIAIELKKDVNSESIKNYLYKNSDLQISYNFNMVAIS
                     DGRPKLMGIRQIIDSYLNHQIEVVANRTKFELDNAEKRMHIVEGLIKALSILDKVIEL
                     IRSSKNKRDAKENLIEVFEFTEEQAEAIVMLQLYRLTNTDIVALEGEHKELEALIKQL
                     RHILDNHDALLNVIKEELNEIKKKFKSERLSLIEAEIEEIKIDKEVMVPSEEVILSMT
                     RHGYIKRTSIRSFNASGVEDIGLKDGDSLLKHQEVNTQDTVLVFTNKGRYLFIPVHKL
                     ADIRWKELGQHVSQIVPIEEDEVVINVFNEKDFNTDAFYVFATQNGMIKKSTVPLFKT
                     TRFNKPLIATKVKENDDLISVMRFEKDQLITVITNKGMSLTYNTSELSDTGLRAAGVK
                     SINLKAEDFVVMTEGVSENDTILMATQRGSLKRISFKILQVAKRAQRGITLLKELKKN
                     PHRIVAAHVVTGEHSQYTLYSKSNEEHGLINDIHKSEQYTNGSFIVDTDDFGEVIDMY
                     IS"
     gene            4098..5558
                     /locus_tag="P6L01_002106"
     CDS             4098..5558
                     /locus_tag="P6L01_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDP3031156.1"
                     /translation="MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSK
                     FVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGG
                     PGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
                     AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLS
                     SLIVPIMAIIYIGMVLVILLFNLDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKR
                     GLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKF
                     GDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRV
                     ILFIFRCLVVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQ
                     KQKKEGKNPVFKPENLEINLFGISAWGANKYKNSDK"
BASE COUNT         1939 a          762 c         1110 g         1768 t
ORIGIN      
        1 tttaattttg atgttttaag cgaacgacta caagagtctg cgttcttatt gaaaaattta
       61 aaaataacgc ttaatgattt acgcagtggt aaagagcgtc aagagcatta ccattatgaa
      121 gaaggaatca aagagtttgt tagttatgtc aatgaaggaa aagaagtttt gcatgacgtg
      181 gctacatttt caggtgaagc aaatggtata gaggtagacg tagctttcca atataatgat
      241 caatattcag aaagtatttt aagttttgta aataatgtac gtactaaaga tggtggtaca
      301 catgaagttg gttttaaaac agcaatgaca cgtgtattta atgattatgc acgtcgtatt
      361 aatgaactta aaacaaaaga taaaaactta gatggtaatg atattcgtga aggtttaaca
      421 gctgttgtgt ctgttcgtat tccagaagaa ttattacaat ttgaaggaca aacgaaatct
      481 aaattgggta cttctgaagc tagaagtgct gttgattcag ttgttgcaga caaattgcca
      541 ttctatttag aagaaaaagg acaattgtct aaatcacttg tgaaaaaagc gattaaagca
      601 caacaagcaa gggaagctgc acgtaaagct cgtgaagatg ctcgttcagg taagaaaaac
      661 aagcgtaaag acactttgct atctggtaaa ttaacacctg cacaaagtaa aaacactgaa
      721 aaaaatgaat tgtatttagt cgaaggtgat tctgcgggag gttcagcaaa acttggacga
      781 gaccgcaaat tccaagcgat attaccatta cgtggtaagg taattaatac agagaaagca
      841 cgtctagaag atatttttaa aaatgaagaa attaatacaa ttatccacac aatcggggca
      901 ggcgttggta ctgactttaa aattgaagat agtaattata atcgtgtaat tattatgact
      961 gatgctgata ctgatggtgc gcatattcaa gtgctattgt taacattctt cttcaaatat
     1021 atgaaaccgc ttgttcaagc aggtcgtgta tttattgctt tacctccact ttataaattg
     1081 gaaaaaggta aaggcaaaac aaagcgagtt gaatacgctt ggacagacga agagcttaat
     1141 aaattgcaaa aagaacttgg taaaggcttc acgttacaac gttacaaagg tttgggtgag
     1201 atgaaccctg aacaattatg ggaaacgacg atgaacccag aaacacgaac tttaattcgt
     1261 gtacaagttg aagatgaagt gcgttcatct aaacgtgtaa caacattaat gggtgacaaa
     1321 gtacaaccta gacgtgaatg gattgaaaag catgttgagt ttggtatgca agaggaccaa
     1381 agtattttag ataattctga agtacaagtg cttgaaaatg atcaatttga tgaggaggaa
     1441 atctagtgag tgaaataatt caagatttat cacttgaaga tgttttaggt gatcgctttg
     1501 gaagatatag taaatatatt attcaagagc gtgcattgcc agatgttcgt gatggtttaa
     1561 aaccagtaca acgtcgtatt ttatatgcaa tgtattcaag tggtaataca cacgataaaa
     1621 atttccgtaa aagtgcgaaa acagtcggtg atgttattgg tcaatatcat ccacatggag
     1681 actcctcagt gtacgaagca atggtccgtt taagtcaaga ctggaagtta cgacatgtct
     1741 taatagaaat gcatggtaat aatggtagta tcgataatga tccgccagcg gcaatgcgtt
     1801 acactgaagc taagttaagc ttactagctg aagagttatt acgtgatatt aataaagaga
     1861 cagtttcttt cattccaaac tatgatgata cgacactcga accaatggta ttgccatcaa
     1921 gatttcctaa cttactagtg aatggttcta caggtatatc tgcaggttac gcgacagata
     1981 taccaccaca taatttagct gaagtgattc aagcaacact taaatatatt gataatccgg
     2041 atattacagt caatcaatta atgaaatata ttaaaggtcc tgattttcca actggtggta
     2101 ttattcaagg tattgatggt attaaaaaag cttatgaatc aggtaaaggt agaattatag
     2161 ttcgttctaa agttgaagaa gaaactttac gcaatggacg taaacagtta attattactg
     2221 aaattccata tgaagtgaac aaaagtagct tagtaaaacg tatcgatgaa ttacgtgctg
     2281 acaaaaaagt cgatggtatc gttgaagtac gtgatgaaac tgatagaact ggtttacgaa
     2341 tagcaattga attgaaaaaa gatgtgaaca gtgaatcaat caaaaattat ctttataaaa
     2401 actctgattt acagatttca tataatttca acatggtcgc tattagtgat ggtcgtccaa
     2461 aattgatggg tattcgtcaa attatagata gttatttgaa tcatcaaatt gaggttgttg
     2521 caaatagaac gaagtttgaa ttagataatg ctgaaaaacg tatgcatatc gttgaaggtt
     2581 tgattaaagc gttgtcaatt ttagataaag taattgaatt gattcgtagc tctaaaaaca
     2641 agcgtgacgc taaagaaaac cttatcgaag tattcgagtt cacagaagaa caggctgaag
     2701 caattgtaat gttacagtta tatcgtttaa caaacactga catagttgcg cttgaaggtg
     2761 aacataaaga acttgaagca ttaatcaaac aattacgtca tattcttgat aaccatgatg
     2821 cattattgaa tgtcataaaa gaagaattga atgaaattaa aaagaaattc aaatctgaac
     2881 gactgtcttt aattgaagca gaaattgaag aaattaaaat tgacaaagaa gttatggtgc
     2941 ctagtgaaga agttatttta agtatgacac gtcatggata tattaaacgt acttctattc
     3001 gtagctttaa tgctagcggt gttgaggata ttggtttaaa agatggtgac agtttactta
     3061 aacatcaaga agtaaatacg caagataccg tactagtatt tacaaataaa ggtcgttatc
     3121 tatttatacc ggttcataaa ttagcagata ttcgttggaa agaattgggg caacatgtat
     3181 cacaaatagt tcctatcgaa gaagatgaag tggttattaa tgtctttaat gaaaaggact
     3241 ttaatacaga tgcattttat gtttttgcga ctcaaaatgg catgattaag aaaagtacag
     3301 tgcctctatt taaaacaacg cgttttaata aacctttaat tgctactaaa gttaaagaaa
     3361 atgatgattt gattagtgtt atgcgctttg aaaaagatca attaattacc gtcattacta
     3421 ataaaggtat gtcattaacg tataatacaa gtgaactatc agataccgga ttaagggcag
     3481 ctggtgttaa atcaataaat cttaaagctg aagatttcgt tgttatgaca gaaggtgttt
     3541 ctgaaaatga tactatattg atggccacac aacgcggctc gttaaaacgt attagtttta
     3601 aaatcttaca agttgctaaa agagcacaac gtggaataac tttattaaaa gaattaaaga
     3661 aaaatccaca tcgtattgta gctgcacatg tagtgacagg tgaacatagt caatatacat
     3721 tatattcaaa atcaaatgaa gaacatggtt taattaatga tattcataaa tctgaacaat
     3781 atacaaatgg ctcattcatt gtagatacag atgattttgg tgaagtaata gacatgtata
     3841 ttagctaaaa actatatgca atcacgaaat taaatgataa aatacagtaa tgttaaattt
     3901 tgactaaatt caagggattt atattaaatg ctgaccaagt acttatcgtt aaattagcga
     3961 tagactttga ccaaactaat ataaatctct tgtattgtat aagaaatgat gatgtatctc
     4021 tatatttact gaagtttcaa ttcggtctaa ttttatagaa ggtgcatgat tcatgtagag
     4081 gatgcgaggg gtttattttg aaagatttcg atagtttaat tcctggatgg tttaaagaat
     4141 ttgtccatgt tggtaccgat ttaatatggt ctcaatattt aattggtcta ttattgacag
     4201 ctggattctt ctttacaatt agttctaaat tcgttcaatt acgaatgtta cctgaaatgt
     4261 ttagagcttt agtagaacgt ccagaaactt tagaagatgg taagaagggt atttcgccat
     4321 tccaagcatt tgcgattagt gctggttcga gagttggtac tggtaatatt gctggtgttg
     4381 cgactgcgat tgttttaggc ggtccaggtg cagtgttttg gatgtgggtt attgcattta
     4441 taggtgcagc gagtgcattt atagaagcga ctttggctca ggtttataaa gtacatgata
     4501 aagatggtgg attccgtggt ggtccagctt actatattac taaaggttta aatcaaaaat
     4561 ggctaggtat cgtatttgcg attttaatta caattacatt tgcatttgta tttaacacag
     4621 tgcaatctaa tacaattgcg gagtcgttaa atacgcaata taatattagt ccagtaatca
     4681 caggtattat tttagcaatc gtaacagcta ttattatatt tggtggtgta cgtagtattg
     4741 ctacgttatc ttcgttaatt gtaccgatta tggctatcat ttacattggt atggttttag
     4801 taatattgct atttaattta gatcaaattg ttcctatgat aggtacgatt attaaaagtg
     4861 catttggtat cgaacaagta actggtggcg ctgtaggtgc tgcggttctt caaggtatca
     4921 aacgtggttt attctctaac gaagctggta tgggttctgc gccgaatgca gcggcaactg
     4981 ctgccgtacc acaccctgtt aagcaaggtt taatccaatc attaggtgtg ttctttgata
     5041 caatgttggt ttgtacagca actgcaatca tgattttact atattcagga ttgaaatttg
     5101 gtgataacgc acctcaaggt gttgcagtta ctcaatcagc acttaatgag catttaggtt
     5161 ctgctggagg tattttctta acaatagcag ttacactgtt tgcattttca tctgttgtag
     5221 gtaattacta ttacggtcaa tctaatattg aatttttatc aacaaatcgt gtgatattat
     5281 ttatctttag atgtcttgtt gtagtacttg tctttgtcgg tgcagttgta aaaacagaaa
     5341 cagtatggaa tacggcagac ttatttatgg gcttaatggc aattgtaaac attatttcca
     5401 ttataggact gtccaatgta gcttttgcat tgatgaaaga ttatcaaaag cagaaaaaag
     5461 aaggcaagaa ccctgtcttt aaacctgaaa acttagaaat taacttattt ggaattagtg
     5521 cttggggcgc taacaaatat aagaactctg ataaataaat atttttaatg taacgattc
//
LOCUS       DAOBBH010000156         5553 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.156, whole genome shotgun sequence.
ACCESSION   DAOBBH010000156 DAOBBH010000000
VERSION     DAOBBH010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.156"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..275)
                     /locus_tag="P6L01_002107"
     CDS             complement(<1..275)
                     /locus_tag="P6L01_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGE"
                     /protein_id="HDP3031157.1"
                     /translation="MKSYKCKGSFLIDSMAGFLLIGLITLLLIPMMNQMQASINHKLQ
                     SIDASKVILTTVSKINKEELKKGVTIGKYDIKQSDQQICAISKNTTS"
     gene            complement(262..708)
                     /locus_tag="P6L01_002108"
     CDS             complement(262..708)
                     /locus_tag="P6L01_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDP3031158.1"
                     /translation="MEKQLQIRKQSAFTMIEMLVVMMLISIFLLLTMTSKGLSNLRVI
                     DDEANIISFITELNYIKSQAIANQGYINVRFYENSDTIKVIENNKIRFLKLKVGKIIN
                     VAKVDIIAFDKKGNINKFGSITIDNNNSIYRIIFHIEKGRIRYEKL"
     gene            complement(686..997)
                     /locus_tag="P6L01_002109"
     CDS             complement(686..997)
                     /locus_tag="P6L01_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDP3031159.1"
                     /translation="MFKFLKKTQAFTLIEMLLVLLIISLLLILIIPNIAKQTAHIQST
                     GCNAQVKMVNSQIEAYALKHNRNPSSIEDLIADGFIKEAQKTCKSGETITISNGEAVA
                     N"
     gene            complement(1011..2081)
                     /locus_tag="P6L01_002110"
     CDS             complement(1011..2081)
                     /locus_tag="P6L01_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDP3031160.1"
                     /translation="MKLQWINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSF
                     QFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIGYSDTIVMQVYLAERFGNIIDVL
                     EETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
                     SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQ
                     LFKTYFVTNELVLFYKNGITLQSIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQIL
                     EKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIKQTQFLQPILFLILG
                     LFIVAIYLVIMLPMFQMMQSIK"
     gene            complement(2053..3027)
                     /locus_tag="P6L01_002111"
     CDS             complement(2053..3027)
                     /locus_tag="P6L01_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDP3031161.1"
                     /translation="MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQ
                     IGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHFNKIYFLRISTLPLSLGQESCVI
                     RIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
                     VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVI
                     QASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLV
                     CEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMDKYI"
     gene            complement(3079..3702)
                     /locus_tag="P6L01_002112"
     CDS             complement(3079..3702)
                     /locus_tag="P6L01_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDP3031162.1"
                     /translation="MRISSLTLGLVDTNTYFIENDKAVILIDPSGESEKIIKKLNQIN
                     KPLKAILLTHAHFDHIGAVDDIVDRFDVPVYMHEAEFDFLKDPVKNGADKFKQYGLPI
                     ITSKVTPEKLNEGSTEIEGFKFNVLHTPGHSPGSLTYVFDEFAVVGDTLFNNGIGRTD
                     LYKGDYETLVDSIQDKIFELEGDLPLFPGHGPYTTVDDEQLNPFLHG"
     gene            complement(3699..4028)
                     /locus_tag="P6L01_002113"
     CDS             complement(3699..4028)
                     /locus_tag="P6L01_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HDP3031163.1"
                     /translation="MAIVDVVVIPVGTEGPSVSKYIADIQKKLQEYKAMGKIDFQLTP
                     MNTLIEGELSDVLEVVQVIHELPFDKGLSRVCTNIRIDDRRDKSRKMNDKLTSVQKHL
                     ENSGENL"
     gene            complement(4028..5014)
                     /locus_tag="P6L01_002114"
     CDS             complement(4028..5014)
                     /locus_tag="P6L01_002114"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDP3031164.1"
                     /translation="MTKIILAADVGGTTCKLGIFTPELEQLHKWSIHTDTSDSTGYTL
                     LKGIYDSFVEKVNENNYNFSNVLGVGIGVPGPVDFEKGTVNGAVNLYWPEKVNVREIF
                     EQFVDCPVYVDNDANIAALGEKHKGAGEGADDVVAITLGTGLGGGIISNGEIVHGHNG
                     SGAEIGHFRADFDQRFKCNCGRSGCIETVASATGVVNLVNFYYPKLTFRSSILELIKE
                     NKVTAKAVFDAAKAGDQFCIFITEKVANYIGYLCSIISVTSNPKYIVLGGGMSTAGPI
                     LIENIKTEYHNLTFAPAQFETEIVQAKLGNDAGITGAAGLIKTYVLDKEGVK"
     gene            complement(5011..5214)
                     /locus_tag="P6L01_002115"
     CDS             complement(5011..5214)
                     /locus_tag="P6L01_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDP3031165.1"
                     /translation="MSRKINNFYDVQQLLKSYGFLIYFKNPEDMYEMIQQEISSLYQY
                     ELLSKEEYLKCTLIINQRRNEQK"
     gene            complement(5195..>5553)
                     /locus_tag="P6L01_002116"
     CDS             complement(5195..>5553)
                     /locus_tag="P6L01_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031166.1"
                     /translation="YNKLIKDDMTSGNYDNAQNIVKQTINKNYADDQTYYLSGMIMAT
                     INSKSEGMTEWERGLRMFPKSGLLNFELAIANRSLNDDEKALKYVRKALNADPKNTDY
                     INLEKELTKSNESKNK"
BASE COUNT         1795 a          969 c          713 g         2076 t
ORIGIN      
        1 taagaagtgg tattttttga aatagcacaa atttgttggt cactttgctt aatatcatat
       61 ttccctatag ttaccccctt cttaagttct tctttattaa ttttagatac agtcgtcaaa
      121 attactttag aagcatcaat tgattgtagt ttatggttta tactcgcttg catttgattc
      181 atcattggta tcaatagtaa tgtaatcaat ccaattagca aaaatccagc catactatct
      241 attaagaatg atcctttaca cttatagctt ttcataacga attcttcctt tttcaatatg
      301 gaatattatt ctataaattg aattgttatt gtcaattgtt atgctaccaa atttattgat
      361 attccctttt ttatcaaagg caataatatc aacttttgca acattaatta ttttgcctac
      421 ttttaatttt agaaatcgta ttttattatt ctctattact ttaatagtgt cactgttttc
      481 ataaaatcta acattgatat atccttgatt tgctatagct tgcgacttaa tataattcaa
      541 ttcagtaata aaagaaatga tatttgcctc atcatctatt actctaagat tgcttaatcc
      601 tttagatgtc attgtcaaaa gtagaaatat actgattaac atcattacca caagcatctc
      661 aatcatagta aatgctgact gctttctaat ttgcaactgc ttctccatta ctaattgtta
      721 ttgtctctcc tgatttacat gtcttttgtg cttcttttat aaaaccatct gcaattaagt
      781 cttcaataga cgatggattt ctattatgtt tcaatgcata cgcttcaatt tgactattaa
      841 ccatttttac ctgtgcatta caacctgttg attgtatgtg agcagtttgt ttagcaatat
      901 ttggaatgat taaaattaat aataaactga tgattaataa cactaatagc atctctatca
      961 atgtaaacgc ttgagttttc ttaagaaatt taaacatata ttaacctcca ttattttata
     1021 ctttgcatca tttgaaacat tggtaacata attactaaat aaattgcgac aataaataaa
     1081 cctaaaatca aaaataaaat aggctgtaaa aactgagtct gttttatcgc tttatcttct
     1141 atttgtttta ctaatatttg cgaatataac tttagttcta cttctagctt ccctctcttt
     1201 tcaccttgta gcacaaactt aattaattga ggcttaaagc attttagttt ttctaaaatt
     1261 tgaggcaaac catatcccat ttctgaataa gttaataagt atttacctag aaactgtcta
     1321 aatggatcac tactatggtt aatataaacg tctactattg attgaagtgt aataccattt
     1381 ttataaaaca acactaattc attagttaca aaataagttt taaataattg gaaatagcct
     1441 gatattagcg gtagtttcat cacaaagttt atcttattga gcatatttaa attgttataa
     1501 attaatttca taataatagc caacatagat actattatga gcattactac aattatagta
     1561 ggtaagctgg taatgaaaaa agacaatgtt ttttgaaaag aagatagttg aatattcata
     1621 gaagtatata attgttgaaa ctgtggaatt actgtgaggt ttaatataat aatcatagca
     1681 ataaagatag aaactagtat taaggggtat tgcagtgtct ttaacaatcg ttgttctgac
     1741 tttctattca ctttcatata atttacggtt tcttctagaa cgtctataat attgccaaat
     1801 ctttctgcca aatatacttg catgacgata gtatcgctat aacctatcag tgataatatt
     1861 tgattgcatg gtgcaccatt tgaaatttca cttagaatgg tggtacctaa ttgcttattt
     1921 ttatatgtca tttgaagatt taaaaattga aaactttgat acagagtgaa accatatttc
     1981 aacaaattac ataaatttga aagtaaatcg atttgttggg ccttacttaa ttgtcgcttc
     2041 ttagaatgta gtttaaatgt atttatccat tgtagtttca caaatgacac ctgcttttgt
     2101 catatcatca agtttatctt ctaagttctt aaatgatgat ggtaatgaat gattatggga
     2161 aaagaaatat cggagttgtt gctgagatag aatttcgcat actaattgtc gctgttgctt
     2221 aatagtagtt acaagtcgtt ggtttataat taagttagtt gcctgtatca attcttgtac
     2281 agaaatgccc atttctaata gccttaaaat agcaccttta caatcagttg catgcaatgt
     2341 agtcagaaca aggtgaccac ttaaactagc ctgtataaca cacttggcaa catctttatc
     2401 tctgatttca cctattaaaa taacatcagg atcacatctt aaaatagctt taaacgaatt
     2461 tacatagtta atgccagctt tatcattcac attaatttgg acgataccag gaatttgcat
     2521 ctctacagga tcctctatag aaattacatt taaattcaag gctttattcg cgtatgagac
     2581 catttgatac attaatgtac tctttcctga accagttggc ccactaaaca atagtaatcc
     2641 ttgtttctta ttcatgaggt gtttaaaatc attgaattta taagttgatt tctgttgttg
     2701 aaaaaattga ggtacaattc tgataacaca actttcttgg ccaagtgaca atggtaaagt
     2761 tgatattctc aaaaaatata ttttattgaa atggtaacta tatcgaccgc tctgtgcgac
     2821 ttgctgtgta gaaacatcaa gcccagcttg aaacttcata taaactaata acttttgata
     2881 aatgctattc ccaatttgtt catactgctc caagttatca ttaattctaa atttaatact
     2941 tacttcattt ttaactggaa taaaatgtac atcactcgct ttcatttcta tcgctttatt
     3001 aattatttct tgaaatagaa tcttcaaaaa aacacctcct acatataatc acgtaggagg
     3061 tgtttttatt acttcaattt aaccgtgtaa aaatggattt aattgttcat catcaaccgt
     3121 cgtatatgga ccatgtccag ggaataaagg taaatcgcct tctaattcaa atattttatc
     3181 ttgaatagaa tcaactagcg tttcataatc acctttatat aaatctgtac gtccgattcc
     3241 attattaaat aatgtatctc caacaactgc gaattcatcg aacacatatg ttaaacttcc
     3301 tggtgaatgt ccaggtgtgt gtaacacatt aaacttaaat ccttctattt ctgtgctacc
     3361 ttcgtttaac ttttcaggag ttaccttact tgtaataatt ggtaatccat attgcttaaa
     3421 tttatctgct ccatttttaa cgggatcttt tagaaaatca aactctgctt catgcatata
     3481 aaccgggaca tcgaatcgat caactatatc atcgactgct ccgatatgat caaagtgtgc
     3541 atgtgttaat aaaatagctt ttaacggttt atttatttgg tttaattttt taataatttt
     3601 ttcactttca cctgaagggt caatcagaat aacagctttg tcattttcga tgaaatacgt
     3661 attagtatca actaagccta aagttaagct tgaaatcctc ataggttttc accactattt
     3721 tctaaatgtt tttgtactga tgttagttta tcattcattt ttctagattt gtctcgtcgg
     3781 tcatcaatac ggatatttgt acaaactcta cttaaacctt tatcaaaagg taattcatgt
     3841 atcacttgca caacttctaa tacatcgctt aattcacctt caattagagt attcattggt
     3901 gttaattgaa aatcaatttt acccattgct ttatattctt gaagtttttt ctgaatatct
     3961 gcaatatatt tactaacact cggaccttcc gttccaactg gaataacaac cacatcaaca
     4021 atagccatta ttttaccccc tctttatcta atacataggt cttgattaat cctgctgctc
     4081 ctgtaatacc tgcatcatta cctaatttcg cttgtacaat ttcagtttca aattgagcag
     4141 gtgcaaatgt taaattatga tattctgttt taatattttc aattaaaata ggtcctgcag
     4201 tagacattcc tccacctaga acgatatatt tcggattact tgtaacacta ataatactac
     4261 ataaatatcc aatatagttt gcaacctttt cagtaatgaa aatacagaat tggtcaccag
     4321 cttttgccgc atcaaaaaca gcttttgctg taaccttatt ttctttaatc aattctaata
     4381 tagaagatct aaacgtcaac ttcggatagt agaagttaac taagttaaca acgcctgtcg
     4441 ctgaagcaac tgtttcaata catccagaac gaccacaatt acatttaaat cgttgatcga
     4501 agtctgctct aaaatgacct atttctgcgc cagagccatt atgaccatgt acgatttcac
     4561 catttgaaat aattcctcca cctagacctg taccaagtgt gatggcaaca acatcatcgg
     4621 caccttcacc agcacctttg tgtttctccc ctaaagcagc tatgttagca tcattatcta
     4681 catacactgg acaatcaacg aattgttcaa aaatctcacg tacattaact ttttctggcc
     4741 aatataagtt tactgctcca tttactgtac ctttttcaaa gtcaacagga cctggtacac
     4801 caatacctac gccaagtaca tttgaaaaat tataattatt ttcatttact ttttcaacaa
     4861 acgaatcata aattcctttc aaaagtgtat atcctgtact atcagatgta tcagtgtgaa
     4921 tagaccattt atgtaattgt tctaattcag gtgtgaaaat acctaattta caagtcgtcc
     4981 cgcctacatc agctgctaaa ataatttttg tcatttctgt tcattccttc tctgattaat
     5041 tatcaacgta catttcaaat attcttcctt agataacagt tcatattgat acaatgatga
     5101 aatctcctgt tgaatcattt cgtacatatc ttctggattt ttaaaatata ttagaaatcc
     5161 gtaacttttc aataactgtt gtacatcata aaagttattt atttttcgac tcatttgatt
     5221 tagtcaactc tttttctaag ttaatataat ctgtattttt agggtctgca tttaatgctt
     5281 tacgcacata ttttaatgct ttttcatcat catttaatga acgatttgct atcgctaact
     5341 caaaatttaa taaacctgat ttaggaaaca ttctaagtcc tcgctcccat tctgtcatac
     5401 cttcggactt agaattgata gtagccataa tcataccact taaataatat gtttgatcat
     5461 cggcataatt tttatttatt gtctgcttca caatattttg agcattatca taattaccac
     5521 tagtcatatc atctttgatc aatttattat aaa
//
LOCUS       DAOBBH010000157         5538 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.157, whole genome shotgun sequence.
ACCESSION   DAOBBH010000157 DAOBBH010000000
VERSION     DAOBBH010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.157"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(39..1886)
                     /gene="typA"
                     /locus_tag="P6L01_002117"
     CDS             complement(39..1886)
                     /gene="typA"
                     /locus_tag="P6L01_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDP3031167.1"
                     /translation="MTNKREDVRNIAIIAHVDHGKTTLVDELLKQSGIFRENEHVDER
                     AMDSNDIERERGITILAKNTAVDYKGTRINILDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLKPVVVVNKIDKPSARPEGVVDEVLDLFIELEANDEQ
                     LEFPVVYASAVNGTASLDPEKQDDNLQSLYETIIDYVPAPIDNSDEPLQFQVALLDYN
                     DYVGRIGIGRVFRGKMRVGDNVSLIKLDGTVKNFRVTKIFGYFGLKRLEIEEAQAGDL
                     IAVSGMEDINVGETVTPHDHQEALPVLRIDEPTLEMTFKVNNSPFAGREGDFVTARQI
                     QERLNQQLETDVSLKVSNTDSPDTWVVAGRGELHLSILIENMRREGYELQVSKPQVII
                     KEIDGVMCEPFERVQCEVPQENAGAVIESLGARKGEMVDMTTTDNGLTRLIFNVPARG
                     MIGYTTEFMSMTRGYGIINHTFEEFRPRIKAQIGGRRNGALISMDQGSASTYAILGLE
                     DRGVNFMEPGTEVYEGMIVGEHNRENDLTVNITKTKHQTNVRSATKDQTQTMNRPRIL
                     TLEEALQFINDDELVEVTPESIRLRKKILNKNVREKEAKRIKQMMQENE"
     gene            1988..2179
                     /locus_tag="P6L01_002118"
     CDS             1988..2179
                     /locus_tag="P6L01_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaF family protein"
                     /protein_id="HDP3031168.1"
                     /translation="MKQKKSKNIFWVFSILAVVFLVLFSFAVGASNVPMMILTFILLV
                     ATFGIGFTTKKKYRENDWL"
     gene            complement(2333..3160)
                     /locus_tag="P6L01_002119"
     CDS             complement(2333..3160)
                     /locus_tag="P6L01_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HDP3031169.1"
                     /translation="MALYGFAQGLIQEAGIRIKQLMEQNLTIETKSNPNDLVTNVDKA
                     TEDFIFDTILETYPNHQVLGEEGHGHDIDTSKGTVWVVDPIDGTLNFVHQQENFAISI
                     GIYIDGKPYAGFVYDVMADVLYHAKVGEGAYRGSQPLKPLNDSNLRQSIIGINPNWLT
                     KPILGEIFKEIVNDSRSARAYGSAALEIVSVATGNLEAYMTPRLQPWDFAGGLVILYE
                     VNGQASNLLGEPLTISGPNSILVGNRGLHQEISNDYLEPYHDTLIQLHEQRFKRKSK"
     gene            3317..3931
                     /locus_tag="P6L01_002120"
     CDS             3317..3931
                     /locus_tag="P6L01_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YktB family protein"
                     /protein_id="HDP3031170.1"
                     /translation="MTKYTFKPKDFKAFNVEGLDARMEALNEYIRPQLHELGEYFSDF
                     FTSQTGETFYPHVAKHARRSVNPPKDTWVAFATSKRGYKMLPHFQIGMFEDQLFVMFG
                     IMHEAKDKATRAKVFERKFKAIQQLPDDYRVCLDHMKPDKPFIKDLTDDDLKEAIQRA
                     INVKKGEFFIARAITPQDKRLKSDKAFIAFLEETFDQFLPFYSA"
     gene            4115..5467
                     /locus_tag="P6L01_002121"
     CDS             4115..5467
                     /locus_tag="P6L01_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDP3031171.1"
                     /translation="MNNKRHSTNEQLSLDEINNTIKFDHRSSNKQKFLSFLGPGLLVA
                     VGYMDPGNWITSMQGGAQYGYTLLFVILISSLSAMLLQSMTVRLGIATGMDLAQMTRH
                     YLSRPIAIIFWIIAELAIIATDIAEVIGSAIALNLLFNIPLIVGALITVLDVFLLLFI
                     MKYGFRKIEAIVGTLIFTVLFIFIFEVYISSPQLNAVLNGFIPHSEIITNNGILYIAL
                     GIIGATIMPHNLYLHSSIVQSRTYSRHNNEEKAQAIKFATIDSNIQLSIAFVVNCLLL
                     VLGASLFFNSNADDLGGFYDLYHALKTEPVLGATMGAIMSTLFAVALLASGQNSTITG
                     TLAGQIVMEGFLRLHIPNWLRRLITRSLAVIPVIVCLIIFKGNAAKIEQLLVFSQVFL
                     SIALPFCLIPLQLATSNKDLMGPFYNKTWVNIISWTLIIILSILNVYLIVQTFQELQS
                     "
BASE COUNT         1786 a          982 c          826 g         1944 t
ORIGIN      
        1 caactttcat cgctcagctt ctcttttatt atgttaaatt attcgttttc ttgcatcatt
       61 tgtttgatac gctttgcttc tttttcacga acatttttgt ttaaaatttt ctttcttaaa
      121 cgtatacttt ctggtgtaac ctcaacaagt tcatcatcat taatgaattg taacgcttct
      181 tccaatgtta gaatacgcgg tctattcatt gtttgtgttt ggtctttcgt tgcagaacgt
      241 acgttagttt gatgttttgt tttagtgatg ttaacagtta aatcattttc acgattatgt
      301 tcaccaacaa tcataccttc ataaacttca gtaccaggtt ccatgaagtt tacacctcta
      361 tcttcaagtc ccaaaatggc ataagtactt gcagaacctt gatccattga aattaatgca
      421 ccattacgac gaccgccaat ttgtgcttta atacgtggtc taaattcttc aaatgtatgg
      481 ttaataatac cgtaacctct tgtcattgac ataaattcag tcgtataacc aatcatacca
      541 cgagccggta cattaaagat taaacgtgta agtccattat cagttgtagt catatcaacc
      601 atttcacctt tacgtgcacc taatgattca ataacagcac ctgcattttc ttgtggcact
      661 tcacattgca cacgttcaaa tggttcacac attacaccat ctatttcttt aataattact
      721 tgtggttttg aaacttgtaa ttcataacct tcacgacgca tattttcaat aaggattgat
      781 aaatgcaatt caccgcgacc agcaactacc catgtatctg gagaatctgt gttagaaact
      841 ttcaaagata catctgtttc taattgttga tttaaacgtt cttgaatttg acgtgctgtt
      901 acaaagtcac cttcacggcc agcaaatgga gaattgttaa ctttaaatgt catttcaaga
      961 gtaggctcat caatacgtag aactggcaat gcttcttgat ggtcatgtgg tgttacagtt
     1021 tcaccaacat taatgtcttc catacctgaa acagcaatta aatctccagc ttgtgcttct
     1081 tcaatttcta aacgttttaa tccaaagtaa ccaaagattt tagttacacg gaagtttttc
     1141 actgtaccgt ctaatttaat tagtgataca ttatctccga cacgcatttt acctctgaat
     1201 acacgaccaa taccaatacg tccaacataa tcattgtagt ccaacaatgc tacttggaat
     1261 tgtaatggct catcactgtt atcaattgga gctggtacat aatcaataat tgtttcatat
     1321 aatgattgta aattatcatc ttgtttttca ggatctaagc tagctgttcc atttactgct
     1381 gaagcataaa caacagggaa ttctaattgt tcatcgtttg cttctaattc aataaataaa
     1441 tctaaaactt catctacaac accctctgga cgtgctgatg gtttatcaat tttattaaca
     1501 acaacaacag gtttcaggtt ttgttctagc gcttttttaa gtacaaaacg tgtttgaggc
     1561 attgtacctt catacgcatc tactactaag acaaccccat caaccatttt cataatacgt
     1621 tctacttctc caccaaagtc tgcatgtcct ggtgtatcca aaatattaat acgtgtacct
     1681 ttataatcaa cagccgtatt tttggctaga atcgtaattc cacgctctct ttcgatatcg
     1741 ttagagtcca ttgcacgttc atcgacatgt tcattttctc tgaatatacc agattgtttt
     1801 aacaactcat ctactaaagt tgttttacca tggtcaacgt gagcaataat tgctatattg
     1861 cggacatctt ctcttttatt agtcatataa atagtttcct tctttcttaa gtaaaatccc
     1921 acttttaatt acaacttttt tattatatca tataattgag aaaagataag caaaaggtgg
     1981 ggataggatg aaacaaaaaa aatctaaaaa tatcttttgg gtattttcta tattagctgt
     2041 tgttttctta gtattattta gttttgctgt tggtgcatca aatgtaccaa tgatgatttt
     2101 aacatttata ttactcgttg caacctttgg aattggattt actacaaaga aaaaatatcg
     2161 agaaaacgat tggctataag ttatatcgac aataacacgc gttgatttaa gaccacacat
     2221 gttcattatg aacatttaaa tggttttata ttggcgcgtg tttttattat ttaaggtata
     2281 aaagcgtaat atgttaccta attgttagga ctacatatta cgcttttggt aattattttg
     2341 attttctttt aaatcgttgt tcatgtaatt gtattaacgt atcatggtag ggctctaaat
     2401 aatcattgct aatttcttga tggagacctc gatttccaac taagattgaa tttggaccac
     2461 taattgttaa tggttctcct agtaaatttg aagcttgtcc atttacttca tataaaataa
     2521 ccaatccgcc agcaaaatcc catggttgaa gtcttggcgt catatatgct tctaaattac
     2581 ctgtagcaac tgaaacgatt tcaagcgctg cactaccata tgcccttgca cttctagaat
     2641 cattaacaat ttctttaaag atttctccta aaattggttt agttaaccag ttcggattga
     2701 tcccaataat gctttgtctt agattagaat cattcaatgg tttcaagggt tggctacctc
     2761 gatatgcgcc ttcccctact ttagcatgat ataagacgtc agccataaca tcatatacaa
     2821 aacctgcata aggtttacca tcgatataaa taccaattga aattgcgaaa ttttcttgtt
     2881 gatgaacaaa attcaatgta ccgtctattg ggtcaacaac ccataccgta cctttggaag
     2941 tatcgatgtc atgaccatgc ccttcttcac ctaatacttg atgattggga tatgtttcta
     3001 aaattgtatc aaaaatgaaa tcttctgttg ctttatctac atttgtaaca aggtcattcg
     3061 gatttgactt tgtttcaatt gttaaatttt gctccatcaa ttgtttaatt ctaattcctg
     3121 cttcttgaat aagtccttgg gcaaatccat aaagtgccat aacatcatct cctaacatct
     3181 ttattataca tcaacatttt ataaaaataa cttcttatga taaaaatgaa aatactaatt
     3241 ttaatgagag cgtcttaagc cgcaattgat aaaacatatg ctacaatatt tttaaacact
     3301 atacaggagg tactctatga caaaatatac atttaaacct aaagatttca aagcgttcaa
     3361 cgtagaaggc ttagacgcac gaatggaagc tttaaacgaa tacatacgac cacaactcca
     3421 tgaattagga gaatatttta gtgacttctt tacaagtcaa actggtgaaa cattttatcc
     3481 tcacgtagca aaacatgcta gaagaagtgt gaatcctcct aaagatacat gggttgcttt
     3541 tgctacaagc aaaagaggct ataaaatgtt acctcatttc caaattggta tgtttgaaga
     3601 tcaactgttt gttatgtttg gaatcatgca tgaagcaaaa gataaagcaa cacgtgcaaa
     3661 agtttttgaa agaaaattta aagctattca acaattacct gatgattatc gtgtttgctt
     3721 agatcatatg aaacctgata aaccatttat taaagattta acggatgatg atttaaaaga
     3781 agcgatacaa agagccatca atgtgaaaaa aggtgaattc tttatagcgc gtgcaatcac
     3841 accacaagat aaaagattaa aaagtgacaa agcatttatt gcatttttag aagaaacctt
     3901 cgatcagttc ttaccatttt attctgcata aataactttg tttaaataat agagcacgta
     3961 atcacatcca tgatttcgtg ctcttttttc ttaatattaa atcgaacgtt caacataata
     4021 attcatactt ttaaaaaaat taaaataaat ttaggttgac ctaaacattt tattaggtta
     4081 ttatattgtc cataagaagt agaggtgagt caaaatgaat aataaacgac attcaacaaa
     4141 tgaacaatta agtttagacg aaataaacaa tacaattaaa ttcgatcatc gcagttcaaa
     4201 taaacagaaa tttttatcat ttcttggacc tgggttatta gtcgctgttg gttacatgga
     4261 tcccggaaac tggataacat caatgcaagg tggcgcccaa tatggctata ctttgctatt
     4321 cgtaattctt atttcaagtt tatcagcaat gttacttcaa agcatgacag tgagattagg
     4381 aatagcaaca ggtatggact tagctcaaat gacacgacat tatttatcaa gacctattgc
     4441 tataatcttt tggatcattg cagaactagc aattatcgct acagatattg ctgaggttat
     4501 tggtagtgct attgctctta atctcctatt taacatacct ttaatcgtcg gtgcactaat
     4561 aactgtactt gatgtatttt tactactatt tataatgaaa tatggtttta gaaaaattga
     4621 agctattgtt ggtacattaa ttttcacagt gttattcatc tttatatttg aagtctatat
     4681 ttcatcacca cagttgaatg ctgtgttaaa tggatttata ccacatagtg aaatcattac
     4741 aaataacggc attctctata ttgcattagg tattattggc gctacaatta tgcctcataa
     4801 tttgtactta cattcatcaa ttgtacaatc tagaacatac tcaagacata acaatgaaga
     4861 aaaggcgcaa gcgattaaat ttgctacgat agattcgaac attcagttat caatcgcatt
     4921 tgtagtcaat tgcttattat tagtgttagg agcatcacta tttttcaact cgaacgctga
     4981 cgatttaggt ggtttctatg atttatatca cgccttaaaa actgaacctg tactaggtgc
     5041 aacaatgggt gcaatcatga gtacattatt tgcagttgca ttattagcgt caggtcaaaa
     5101 ttcaacgatt actggtactt tagcaggaca aattgtaatg gaaggatttt taagattaca
     5161 cataccaaat tggttaagac gtttaattac acgttccctt gctgtcattc ctgttatcgt
     5221 atgcttaatc atttttaaag gtaatgcagc taaaattgag caactacttg tattctctca
     5281 agtattttta agtattgctc tgccattctg tttaatacca ttacaattag ctactagtaa
     5341 taaagacttg atgggacctt tctataataa aacatgggtt aatatcattt catggacact
     5401 tattattatt ctaagtattt taaatgtcta tttaatcgtt caaacattcc aagaactaca
     5461 aagttaaaaa taaaaccatt aaaatgttgg ttgtatctaa taattaacaa cttaacattt
     5521 taatggtttt tttgctat
//
LOCUS       DAOBBH010000158         5439 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.158, whole genome shotgun sequence.
ACCESSION   DAOBBH010000158 DAOBBH010000000
VERSION     DAOBBH010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.158"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..241)
                     /locus_tag="P6L01_002122"
     CDS             complement(<1..241)
                     /locus_tag="P6L01_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HDP3031172.1"
                     /translation="MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANS
                     IWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDK"
     gene            407..700
                     /gene="csoR"
                     /locus_tag="P6L01_002123"
     CDS             407..700
                     /gene="csoR"
                     /locus_tag="P6L01_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-sensing transcriptional repressor CsoR"
                     /protein_id="HDP3031173.1"
                     /translation="MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDV
                     LTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQEEAMEELLVTFQKLIKD"
     gene            712..921
                     /locus_tag="P6L01_002124"
     CDS             712..921
                     /locus_tag="P6L01_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDP3031174.1"
                     /translation="MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVT
                     VSYETPANLNSIEKEIYDEGYKIVF"
     gene            1049..1186
                     /locus_tag="P6L01_002125"
     CDS             1049..1186
                     /locus_tag="P6L01_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031175.1"
                     /translation="MKRPEKIQNVVKLLSSLGVNIKKTKSRLDIINTLPASNKVSHEL
                     K"
     gene            complement(1450..2652)
                     /locus_tag="P6L01_002126"
     CDS             complement(1450..2652)
                     /locus_tag="P6L01_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDP3031176.1"
                     /translation="MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQ
                     YSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPIT
                     PIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKII
                     GVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGI
                     LYAPALIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKAIGSGQLLGKGYNHG
                     EVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGF
                     VTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYV
                     DLYHPKSN"
     gene            2970..4040
                     /locus_tag="P6L01_002127"
     CDS             2970..4040
                     /locus_tag="P6L01_002127"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDP3031177.1"
                     /translation="MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYIT
                     NDGDWRKQNNITAEIKSTDELHLENGEALEISQLLKESSSGQPYDAVFPLLHGPNGED
                     GTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEH
                     NILKLVNDKLNYPVFVKPANLGSSVGISKCNNEAELKEGIKEAFQFDRKLVIEQGVNA
                     REIEVAVLGNDYPEATWPGEVVKDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNM
                     ALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYPKLWENMGLSYPELI
                     TKLIELAKERHQDKQKNKYKID"
     gene            4055..5413
                     /locus_tag="P6L01_002128"
     CDS             4055..5413
                     /locus_tag="P6L01_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDP3031178.1"
                     /translation="MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIP
                     FKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVSGPIIWVEDTLTALQQLAQAYLR
                     HVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
                     SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDN
                     GTFIYDGDEPLLKPHVKEVENAKCISIGVATDNALVCSVDDRDTTGISFTINNKEHYD
                     LPILGKHNMKNATIAIAVGHELGLTYNTIYQNLKNVSLTGMRMEQHTLENDITVINDA
                     YNASPTSMRAAIDTLGTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTF
                     GNEAKYIYDSGQQHVEKAQHFNSKDDMIEVLTSDLKAHDRVLVKGSRGMKLEEVVNAL
                     IS"
BASE COUNT         2054 a          754 c          993 g         1638 t
ORIGIN      
        1 ccttatcaat tttgaaaatt tggtcacgtt gaatttgtcg tcctaatagt aagtataaaa
       61 tgaagccgaa taaaggcaag aaaactaaga ctagtaacca agcccagata gaattggcag
      121 aacgtctttc catgaaaata atggtaaagg cgaataataa gtttaaaata aatgcaccaa
      181 taaatattga atttaaaata atattagaat gcttgagtgc aatggataat aactctatca
      241 tttgaaacct cccatcgaaa atcttaaacg agtagtatat cttattaatt gtatcatgaa
      301 gcaatttaaa tatcatgaca atttagtttg aaaaacacac tttgtgttga cgttgtgttt
      361 tatgggatat agtatagtaa ggaatgtaaa tgaaggagtg aatgctatga ctgaacaaga
      421 taatgcacat cattctgaac aaataaaaac gaatcttaaa tcacgtttaa atcgaattga
      481 aggacaagtg agagcgatta atcgcatgat tgaagaagat gtctattgtg atgatgtcct
      541 tacgcaaata agagcgacac gttcggcgtt aaacagtgtt gcgataaagt tattagaaca
      601 acatatgaaa agttgtatta tgaataaagt taatcaaggt gctcaggaag aggcaatgga
      661 agagttatta gtgacttttc aaaaattgat taaagactaa aggagtttaa gatgatacat
      721 caaaatacga tttacacagc gggaattgaa acagaagaac aagtaagtca attgacagaa
      781 cgcatttcaa atatgatagg tgttcatcaa gtgaatatta atataataga tggtcaagta
      841 actgtatcgt atgagacacc agcaaatttg aatagtattg aaaaagaaat ctatgatgaa
      901 ggatacaaaa ttgtatttta gggtataatg tagagtgcgc tatagatttt aattttgaaa
      961 ataaattaaa aattttgtaa atgatgtagt aaaggtatgt cgaatagggt aagataatat
     1021 tgaaagcgat ccaaacgagg tgataataat gaaacgtcct gaaaagattc aaaatgtagt
     1081 caaactattg tcatcattag gtgtgaatat taaaaaaact aaatctcgtt tagacattat
     1141 taatactttg ccagcatcta ataaagtaag tcacgaatta aaataattga ctgtgattgt
     1201 gaatcacaca ccgttaaagc tatcgtcgga ctattcgatg atagcttttt tgactggatt
     1261 tactaaaatg aaagcaagta ctatataaca aaatccctct ttagagtgta cattttacaa
     1321 tgtgtacttc aaagagggat tgttattatc gagtaatatc tatatatgac attttaaatc
     1381 gacgacgtaa atagactata taacgtcatc ttgaaaagtt atgatatttg aaactcaaaa
     1441 tagtttaaat taattacttt ttggatggta taaatcgaca tatcgttttg gttcatgata
     1501 ataaattgat aagactatac ctattccagt catcatactc catagcgcac taccaccata
     1561 actaataaat ggtaatggaa taccagtgat tggtaacaac tgaattgtca taccaatatt
     1621 ttgtaaaata tggaacacaa gtaaagtgac gaaaccaacg ataaagattt tgttaaattg
     1681 atcttcaatt ttcgcagcta atcttattag atggaagatt aaaaataaaa atattaagat
     1741 caatatgaca gaaccgataa agccaagttc ctctccaatc actgaaaaga taaagtcagt
     1801 atgattttca ggtatataaa cttcaccgtg attgtatcct ttacctagta actgtccaga
     1861 accgatagct ttaagtgatt cagttaaatg atagccatca ccactactat atgtataggg
     1921 gtcaagccat gaattgattc gtcccatttg atacagttgg acacctaata aattttcaat
     1981 taatgcgggt gcatatagaa tacctaaaat gactgtcatt gcaccaacaa tacctgtaat
     2041 aaagataggt gctaagatac gccatgttat accacttact aacatcacac ctgcaataat
     2101 agcagctaat actaatgtag ttcctaggtc attttgcagt aatattaaaa tacttggtac
     2161 taacgagaca ccaataattt tgaaaaataa taacaaatca ctttggaatg atttattgaa
     2221 tgtgaattga ttatgtctag aaacgacacg cgctaatgct aaaattaaaa taattttcat
     2281 gaattcagat ggctgaatac tgatagggcc aaacgtgtac caacttttgg caccattgat
     2341 aataggtgta ataggtgact caggaataac gagcaagcct attaataata gacagattaa
     2401 gaaatacaat aaatatgtat aatgtttaat ctttttaggt gaaataaaca tgatgatacc
     2461 tgcaaaaatt gcacctaaaa tgtaataaaa aatttgtctg ataccgaaat tagcactgta
     2521 ttgaccaccg cccattgccg agttaataag cagaacactg aaaattgcta aaacagctat
     2581 agtggctact aatacccagt ctactttgcg aagccaatgc ttatccggct gttgacgaga
     2641 tgaataattc attgcaaact ccttttatac tcactaatgt ttatatcaat tttacatgac
     2701 tttttaaaaa ttagctagaa tatcacagtg atatcagcta tagatttcaa tttgaattag
     2761 gaataaaata gaagggaata ttgttctgat tataaatgaa tcaacataga tacagacaca
     2821 taagtcctcg tttttaaaat gcaaaatagc attaaaatgt gatactatta agattcaaag
     2881 atgcgaataa atcaattaac aataggacta aatcaatatt aatttatatt aaggtagcaa
     2941 accctgatat atcattggag gaaaacgaaa tgacaaaaga aaatatttgt atcgtttttg
     3001 gagggaaaag tgcagaacac gaagtatcga ttctgacagc acaaaatgta ttaaatgcaa
     3061 tagataaaga caaatatcat gttgatatca tttatattac caatgatggt gattggagaa
     3121 agcaaaataa tattacagct gaaattaaat ctactgatga gcttcattta gaaaatggag
     3181 aggcgcttga gatttcacag ctattgaaag aaagtagttc aggacaacca tacgatgcag
     3241 tattcccatt attacatggt cctaatggtg aagatggcac gattcaaggg ctttttgaag
     3301 ttttggatgt accatatgta ggaaatggtg tattgtcagc tgcaagttct atggacaaac
     3361 ttgtaatgaa acaattattt gaacatcgag ggttaccaca gttaccttat attagtttct
     3421 tacgttctga atatgaaaaa tatgaacata acattttaaa attagtaaat gataaattaa
     3481 attacccagt ctttgttaaa cctgctaact tagggtcaag tgtaggtatc agtaaatgta
     3541 ataatgaagc ggaacttaaa gaaggtatta aagaagcatt ccaatttgac cgtaagcttg
     3601 ttatagaaca aggcgttaac gcacgtgaaa ttgaagtagc agttttagga aatgactatc
     3661 ctgaagcgac atggccaggt gaagtcgtaa aagatgtcgc gttttacgat tacaaatcaa
     3721 aatataaaga tggtaaggtt caattacaaa ttccagctga cttagacgaa gatgttcaat
     3781 taacgcttag aaatatggca ttagaggcat tcaaagcgac agattgttct ggtttagtcc
     3841 gtgctgattt ctttgtaaca gaagacaacc aaatatatat taatgaaaca aatgcaatgc
     3901 ctggatttac ggctttcagt atgtatccaa agttatggga aaatatgggc ttatcttatc
     3961 cagaattgat tacaaaactt atcgagcttg ctaaagaacg tcaccaggat aaacagaaaa
     4021 ataaatacaa aattgactaa ctgaggttgt tattatgatt aatgttacat taaagcaaat
     4081 tcaatcatgg attccttgtg aaattgaaga tcaattttta aatcaagaga taaatggagt
     4141 cacaattgat tcacgagcaa tttctaaaaa tatgttattt ataccattta aaggtgaaaa
     4201 tgttgacggt catcgctttg tctctaaagc attacaagat ggtgctgggg ctgcttttta
     4261 tcaaagaggg acacctatag atgaaaatgt aagcgggcct attatatggg ttgaagacac
     4321 attaacggca ttacaacaat tggcacaagc ttacttgaga catgtaaacc ctaaagtaat
     4381 tgccgtcaca gggtctaatg gtaaaacaac gactaaagat atgattgaaa gtgtattgca
     4441 taccgaattt aaagttaaga aaacgcaagg taattacaat aatgaaattg gtttaccttt
     4501 aactattttg gaattagata atgatactga aatatcaata ttggagatgg ggatgtcagg
     4561 tttccatgaa attgaatttc tgtcaaacct cgctcaacca gatattgcag ttataactaa
     4621 tattggtgag tcacatatgc aagatttagg ttcgcgcgag gggattgcta aagctaaatc
     4681 tgaaattaca ataggtctaa aagataatgg tacgtttata tatgatggcg atgaaccatt
     4741 attgaaacca catgttaaag aagttgaaaa tgcaaaatgt attagtattg gtgttgctac
     4801 tgataatgca ttagtttgtt ctgttgatga tagagatact acaggtattt catttacgat
     4861 taataataaa gaacattacg atctgccaat attaggaaag cataatatga aaaatgcgac
     4921 gattgccatt gcggttggtc atgaattagg tttgacatat aacacaatct atcaaaattt
     4981 aaaaaatgtc agcttaactg gtatgcgtat ggagcaacat acattagaaa atgatattac
     5041 tgtgataaat gatgcctata atgcaagtcc tacaagtatg agagcagcta ttgatacact
     5101 gggtactttg acagggcgtc gcattctaat tttaggagat gttttagaat taggtgaaaa
     5161 tagcaaagaa atgcatatcg gtgtaggtaa ttatttagaa gaaaagcata tagatgtgtt
     5221 gtatacgttt ggtaatgaag cgaagtatat ttatgattcg ggccagcaac atgtcgaaaa
     5281 agcacaacac ttcaattcta aagacgatat gatagaagtt ttaacaagcg atttaaaagc
     5341 gcatgaccgt gtattagtta aaggatcacg tggtatgaag ttagaagaag ttgtaaatgc
     5401 tttaatttca tagagattag tcgagcgacc ttttactta
//
LOCUS       DAOBBH010000159         5342 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.159, whole genome shotgun sequence.
ACCESSION   DAOBBH010000159 DAOBBH010000000
VERSION     DAOBBH010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.159"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            89..1048
                     /locus_tag="P6L01_002129"
     CDS             89..1048
                     /locus_tag="P6L01_002129"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDP3031179.1"
                     /translation="MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVK
                     ADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIM
                     KSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
                     QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTR
                     DAAYEIIQAKGSTYYGIALALMRISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPT
                     LVNQHGAVKIYEMPLSAEEQALFDKSVKILEDTFDSIKYLLED"
     gene            1326..2030
                     /gene="budA"
                     /locus_tag="P6L01_002130"
     CDS             1326..2030
                     /gene="budA"
                     /locus_tag="P6L01_002130"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDP3031180.1"
                     /translation="MTNVLYQHGTLGTLMAGLLEGTATINELLEHGNLGIATLTGSDG
                     EVIFLDGKAYHANEHKEFIELKGDEKVPYASITNFKASKTFPLQQLSQDDVFAQIKNE
                     MLSENLFSAVKIYGTFKHMHVRMMPAQQPPYTRLIDSARRQPEEKRQDIRGAIVGFFT
                     PELFHGVGSAGFHIHFADDERAYGGHVLDFEVDDVVVEIQNFETFQQHFPVNNETFVK
                     AKIDYKDVAEEIREAE"
     gene            complement(2116..2976)
                     /locus_tag="P6L01_002131"
     CDS             complement(2116..2976)
                     /locus_tag="P6L01_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDP3031181.1"
                     /translation="MLSVAIIGPGAVGTTIAYELQQSLPHTTLIGRHAKTITYYTVPH
                     APAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTYLAHEDTLIILAQNGYGQLE
                     HIPFKNVCQAVVYISGQKKGDVVTHFRDYQLRIQDNALTRQFRDLVQDSQIDIVLEAN
                     IQQAIWYKLLVNLGINSITALGRQTVAIMHNPEIRILCRQLLLDGCRVAQAEGLNFSE
                     QTVDTIMTIYQGYPDEMGTSMYYDIVHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIY
                     SFLRAYQQNM"
     gene            3049..3867
                     /gene="panB"
                     /locus_tag="P6L01_002132"
     CDS             3049..3867
                     /gene="panB"
                     /locus_tag="P6L01_002132"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDP3031182.1"
                     /translation="MKTVSQLIDMKQKQTKISMVTAYDFPSAKQVEAAGIDMILVGDS
                     LGMTVLGYESTVQVTLADMIHHGRAVRRGAPNTFVVVDMPIGAVGISMTQDLNHALKL
                     YQETNANAIKAEGAHITPFIEKATAIGIPVVAHLGLTPQSVGVMGYKLQGATKEAAEQ
                     LILDAKNVEQAGAVALVLEAIPNDLAEEISKHLTIPVIGIGAGKGTDGQVLVYHDMLN
                     YGVEHKAKFVKQFADFSVGVDGLKQYDQEVKSGAFPSEEYTYKKKIMNEVNNND"
     gene            3860..4711
                     /locus_tag="P6L01_002133"
     CDS             3860..4711
                     /locus_tag="P6L01_002133"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDP3031183.1"
                     /translation="MTKLITTVKEMQHIVKAAKRSGTTIGFIPTMGALHDGHLTMVRE
                     SVSTNDITVVSVFVNPLQFGPNEDFDAYPRQIDKDLELVSEVGADIVFHPAVEDIYPG
                     ELGIDVKVGPLADVLEGAKRPGHFDGVVTVVNKLFNIVMPDYAYFGKKDAQQLAIVEQ
                     MVKDFNHAVEIIGIDIVREADGLAKSSRNVYLTEQERQEAVHLSKSLLLAQALYQDGE
                     RQSKVIIDRVTEYLESHISGRIEEVAVYSYPQLVEQHEITGRIFISLAVKFSKARLID
                     NIIIGAE"
     gene            4713..5096
                     /locus_tag="P6L01_002134"
     CDS             4713..5096
                     /locus_tag="P6L01_002134"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDP3031184.1"
                     /translation="MIRTMMNAKIHRARVTESNLNYVGSITIDSDILEAVDILPNEKV
                     AIVNNNNGARFETYVIAGERGSGKICLNGAASRLVEVGDVVIIMTYAQLNEEEIKNHA
                     PKVAVMNEDNVIIEMIHEKENTIVL"
BASE COUNT         1835 a          747 c         1124 g         1636 t
ORIGIN      
        1 ataaaattgt taatctttaa tttcagtata gatattttta cgtgtagtca cgtgtaaaat
       61 aaattcaatt aggttaggag acataattat gaaaacattt ggtaaaaagg ttgtattaat
      121 cggagatgga tctgtaggat caagctatgc ctttgcaatg gttacgcaag gtgttgctga
      181 tgaatttgta attattgaca ttgcaaaaga caaagtaaaa gcagatgttc aagatttaaa
      241 ccatggtaca gtccacagtc cttcaccagt tgatgtgaaa gcaggtgaat acgaagactg
      301 taaagatgca gatttagttg ttattacagc tggtgcacct caaaagccag gtgaaacacg
      361 tttacaatta gttgaaaaaa atactaagat tatgaagagc attgttaaga gtgttatgga
      421 tagcggcttt gatggatatt tcttaatcgc ggcaaaccct gttgacattt taacaagatt
      481 tgtaaaagaa tatactggtt taccagcaga gcgtgttatc ggttcaggta ctgtattgga
      541 cagtgcacgt ttacaatatt taattagcca agaacttggt gttgcacctt caagtgttga
      601 cgctagtatt attggtgagc atggtgatac tgaacttgca gtttggtcac aagcaaatgt
      661 agcaggtatt tcagtatatg acacattaaa agaacaaact ggtagcgaag ctaaagcgga
      721 agaaatttat gtaaatacac gtgacgctgc ttatgaaatt atccaagcta aagggtcaac
      781 atactatgga attgcattag cattgatgcg catttcaaaa gccattttaa ataatgaaaa
      841 taatgtctta aatgtttcta tacaattaga tggtcaatat ggtggtcaca aaggcgttta
      901 cctaggtgta ccaacattag ttaaccaaca tggcgcagtt aaaatttatg aaatgccatt
      961 aagtgccgaa gaacaagcgt tgttcgataa atctgttaaa atattagaag atacatttga
     1021 ttcaattaaa tatttattag aagactaagc ctattttaag tattaattag aaatatatta
     1081 atggtaagag gatctattag tgttgcagta acatgtggca ctgatagatc cattttttta
     1141 gcaagcattg atagaggcaa tacttttgcg taaaataata attgaataaa gtattatgac
     1201 tcgtgaaaat ttagtgacat gaatcgaaat gccgaggcgt cattgagtat aaaggaaagt
     1261 agttctgcat taagcacgaa gtagagcatg acaacgagga ataactatag ggagatggtt
     1321 ttggaatgac gaatgtcttg tatcaacatg gtacattagg tacgttaatg gctggcttac
     1381 tagaaggaac agctacaatt aatgaattgt tagaacatgg gaatttaggg attgcaacgt
     1441 taacagggtc tgatggcgaa gtaatatttt tagacgggaa ggcatatcat gctaacgagc
     1501 ataaagaatt tatagaatta aaaggcgatg agaaagtacc gtatgcatcg attactaatt
     1561 ttaaagcgag taagacattt ccattgcaac aattatcaca agatgatgta ttcgcacaaa
     1621 ttaaaaatga aatgttaagt gagaatttat tttcggctgt taaaatttat ggcacattta
     1681 aacatatgca tgtacgaatg atgcctgctc agcaaccgcc atatacacgt ttgattgatt
     1741 cagcacgcag acaacctgag gaaaaaagac aagatattcg tggtgccatt gttggatttt
     1801 ttacaccaga attatttcat ggcgtagggt ctgctggttt tcatatacat tttgcggatg
     1861 atgaaagagc ttatggtgga catgttcttg actttgaagt ggatgacgtt gtcgttgaga
     1921 tacaaaactt tgaaacattc caacaacatt tcccggtaaa taacgagacg tttgttaaag
     1981 cgaaaataga ctataaagat gtggcagaag aaattagaga agctgaatag tctaatataa
     2041 ttaaaagacc ttagcgatat taggaacagg tggttctaaa tgcatcgtta aggtcttttt
     2101 atattatata tgtgcttaca tattttgttg ataagcgcgt aaaaagctat aaatagtatc
     2161 aagatatggg gtgtcaagat tgtgttcacg tgcacgtcga taaataaagc cttgaatcgc
     2221 ttcgacttca agaggttgct gatgcacaat gtcgtaatac atgctcgttc ccatttcgtc
     2281 gggataacct tgatatatag tcataatagt atcgacagtt tgttctgaaa agtttaaccc
     2341 ctctgcctgt gcaacacgac aaccatctag taataactgt ctacacagta tacgtatttc
     2401 aggattatgc atgattgcaa ctgtctgtct cccgagtgct gtgatagaat taatgcccaa
     2461 gttaactaac aatttatacc aaatagcttg ttgaatattt gcttctaata caatgtcgat
     2521 ttgactgtct tggaccaaat ctctaaattg tcgagttaat gcattatctt gtatacgtaa
     2581 ttgataatct ctgaagtgcg taacaacatc gcctttcttt tgaccactta tatagacaac
     2641 tgcttggcat acatttttaa atggaatatg ttcgagttga ccataaccat tttgggctaa
     2701 aatgatgagc gtgtcctcat gtgcaagata agttaaatga ggaataacgg catcaagttg
     2761 atgtgttttg actgcaataa taataacatc aaatgtgttt gtgacatctt cataaccttt
     2821 cacaacaata tcttgtgcag gtgcatgtgg tacagtataa tatgttattg ttttggcgtg
     2881 tctcccgata agcgttgtat gaggcaatga ttgttgtaat tcataggcaa tagttgtacc
     2941 gacagcgcct gggccgataa tcgcaactga taacatcttc ttctttcctt tctataaaag
     3001 attcattata ctatttctaa taatactgca aaaagaggtt gaataacatt gaaaacagta
     3061 agtcaattaa tagatatgaa acaaaagcaa actaaaattt ctatggtaac agcttatgat
     3121 tttccaagtg ctaaacaagt ggaagcagcg ggtattgata tgattcttgt tggggattca
     3181 cttggtatga ctgtattagg ttatgaaagt accgtacaag tgacgttagc agatatgatt
     3241 catcacggtc gtgcagtaag aagaggcgca ccaaatacat ttgtagttgt tgatatgccg
     3301 attggtgcag taggcatctc tatgacacaa gacttaaatc atgctctaaa gctttatcag
     3361 gagacgaatg caaatgcaat taaagcagaa ggagcgcata ttacaccatt tattgaaaaa
     3421 gcaacggcaa ttggtattcc tgttgttgca catttaggat taacacctca aagtgttggc
     3481 gttatggggt ataaattaca aggtgcaacg aaagaagcag cggagcaact tattttagat
     3541 gcgaaaaatg tagaacaagc tggtgcagtt gcgttagtac ttgaagcaat tccaaatgat
     3601 ttagcagagg aaattagcaa gcacttaaca attccagtca ttggtattgg tgcaggaaaa
     3661 ggtacagatg gtcaagtatt ggtttatcac gatatgttaa attatggtgt tgaacataaa
     3721 gcgaaatttg tgaagcaatt tgctgatttt agtgttggtg tggatggttt aaaacaatac
     3781 gatcaagaag ttaaatctgg tgcatttcca tcagaagaat atacgtataa aaagaaaatt
     3841 atgaatgagg ttaataacaa tgactaagct gattactacg gtaaaagaga tgcaacacat
     3901 tgttaaagca gccaaacgct caggaacaac gattggtttt atcccaacaa tgggtgcctt
     3961 acatgatgga catttaacga tggtacgcga atcagttagt acaaatgata ttacagttgt
     4021 gagtgtattt gttaatccat tacaatttgg tccaaacgaa gatttcgatg cttatccgag
     4081 acaaattgat aaagatttag aattagtatc agaagtaggg gcggacattg tttttcatcc
     4141 tgcagtagaa gatatatatc caggtgaatt aggtattgat gtcaaagtag gtccattagc
     4201 tgatgtgtta gaaggagcga agcgtccagg gcattttgat ggggtggtaa cagtagttaa
     4261 taagctattt aatattgtta tgccggatta tgcttatttt ggtaaaaaag atgcccagca
     4321 attggctatt gtcgagcaaa tggtaaaaga cttcaatcat gccgttgaaa ttattggtat
     4381 tgatatcgtt cgagaagcag atggtttggc gaaaagttca agaaatgttt atttaacgga
     4441 gcaagaacga caagaagcgg tacatttaag taaaagtttg ctattagcac aagcgttgta
     4501 tcaagacggt gaacgtcaaa gtaaagtaat tatagacagg gttactgaat atcttgaatc
     4561 acatataagt ggacgcattg aagaagttgc tgtttatagt tatcctcaat tagtagaaca
     4621 acacgaaata actggacgca tatttatctc gttagctgtc aaattttcaa aagcgcgttt
     4681 aatagataat ataataattg gagctgaata aagtgataag aacaatgatg aatgctaaaa
     4741 ttcatagagc aagagtgact gagtcaaatt taaattatgt aggtagtatt acgattgatt
     4801 cagatatttt ggaagcggta gacatcttgc caaatgaaaa agtagccatc gtgaataata
     4861 ataatggtgc acgttttgaa acatacgtaa tagctggtga aagaggtagt ggtaaaattt
     4921 gtttgaacgg tgcagcatca agacttgttg aagtaggcga tgtagtcata atcatgactt
     4981 atgcacagtt aaatgaagaa gaaattaaaa accatgctcc taaagtagca gtaatgaatg
     5041 aagacaatgt cattattgaa atgattcatg aaaaagaaaa tacgatagtg ttataaaata
     5101 gtataagtta gaaacctcta gtagataaca atgtagttat ccattagggg tttttatttt
     5161 tatgccaatt gggttagcgt aatagaagta aaggtattat cgttgcaatt ttatcagtgt
     5221 taaatgatgg acatatttat aaagaaatag ttttgcgaat tgattatcta aaacagtatt
     5281 cagaatttat ttggtagaaa atagaattct taacttaacg agataagtta atgacatgtt
     5341 gt
//
LOCUS       DAOBBH010000160         5200 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.160, whole genome shotgun sequence.
ACCESSION   DAOBBH010000160 DAOBBH010000000
VERSION     DAOBBH010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.160"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            207..521
                     /locus_tag="P6L01_002135"
     CDS             207..521
                     /locus_tag="P6L01_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDP3031185.1"
                     /translation="MTYKELATFLKVLSDSSRLEILDLLSCGELCACDLLAHFQFSQP
                     TLSYHLKALVKTNLVTTRKIGNKHLYQLNHNIFESVINNLSKVHTSNQRCFCHNLKTG
                     EC"
     gene            521..1813
                     /gene="arsB"
                     /locus_tag="P6L01_002136"
     CDS             521..1813
                     /gene="arsB"
                     /locus_tag="P6L01_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HDP3031186.1"
                     /translation="MMTTLATLIFLVTLLFVLWQPKGLDIGITALTGAFIAVITGVVS
                     FSDVFEVTGIVWNATLTFVSVILISLILDKVGLFEWSAIHMLHASKGNGLKMFVYIIL
                     LGAIVAAFFANDGAALILTPIVLAMVKNIGFSKRAIFPFIIASGFIADTTSLPLIVSN
                     LVNIISADYFHVGFVRYFSRMIIPNLFSLLASIIVLWLYFRKAIPKTFDDNNIKHPKD
                     AINDLKLFKISWIVLVILLFGYLISEFTKIPVSIFTGIIAFIFLMLARKSNAVNIKQV
                     IKGAPWNIVLFSIGMYIVVFGLRNAGITLILAKILEYISNYGLFSTILGMGFISAFLS
                     SIMNNMPTVLIDAIAIGQSNVHGMLKEGLIYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKDVKISWGTYFKTGIIITIPVLFITLIGLYLTLIIF"
     gene            complement(1900..2754)
                     /locus_tag="P6L01_002137"
     CDS             complement(1900..2754)
                     /locus_tag="P6L01_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDP3031187.1"
                     /translation="MNKHKKGSIFGIIGLVVIFAVVSFLFFSMISDQIFFKHVKSDIK
                     IEKLNVTLNDAAKKQINNYTSQQVSNKKNDAWRDASATEIKSAMDSGTFIDNEKQKYQ
                     FLDLSKYQGIDKNRIKRMLVDRPTLLKHTDDFLKAAKDKHVNEVYLISHALLETGAVK
                     SELANGVEIDGKKYYNFYGVGALDKDPIKTGAEYAKKHGWDTPEKAISGGADFIHKHF
                     LSSTDQNTLYSMRWNPKNPGEHQYATDIKWAESNATIIADFYKNMKTEGKYFKYFVYK
                     DDSKHLNK"
     gene            complement(3030..3254)
                     /locus_tag="P6L01_002138"
     CDS             complement(3030..3254)
                     /locus_tag="P6L01_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031188.1"
                     /translation="MDYAHLNLEHFFARNDDLDVIRDRADFVMINNFTNEMMYRDGQI
                     EGTIDLNQYYYKNRSNAASFIMMDYKKETK"
     gene            3453..3923
                     /gene="sigS"
                     /locus_tag="P6L01_002139"
     CDS             3453..3923
                     /gene="sigS"
                     /locus_tag="P6L01_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigS"
                     /protein_id="HDP3031189.1"
                     /translation="MKFNDVYNKHHKIIHHLLKKYNISYNYDEYYQLLLIKMWQLSQI
                     YKPSSKQSLSSFLFTRLNYYLIDLFRQQNQLKDVILCENNSPTLTEQPTYFNEHDLRL
                     QDIFKLLNHRERLWLKLYLEGYKQFEIAEIMSLSLSTIKLIKMSVKRKCQHNFN"
     gene            4036..4479
                     /locus_tag="P6L01_002140"
     CDS             4036..4479
                     /locus_tag="P6L01_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HDP3031190.1"
                     /translation="MQDNSTKYLLYIQTATSNHLETNCVFLHCDYILKVPINKLVSYY
                     AKLHLSSQSVLIETAKNILNINKLVPIYINPKTILFPLKHKRAPIQIYINAHYIVGMT
                     AIENSTLIHFQEGIQLEVDEPFSLVSKKCHESLALKHFIENTISN"
     gene            complement(4466..4909)
                     /locus_tag="P6L01_002141"
     CDS             complement(4466..4909)
                     /locus_tag="P6L01_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031191.1"
                     /translation="MNKASFDKKVKKQLWFLNKKEKQALDQRLSSISDDDSVNLNKPV
                     TFANAYLRQNVFRNKETKSYSMFVTLVVMMFAYVALLGLFLFGLITSLSGVQFFVSPK
                     VDLSTTVVILTIIGAILLMIVSIYFIKIVTSYFTKKLLEIKFNSK"
BASE COUNT         1748 a          839 c          676 g         1937 t
ORIGIN      
        1 ccatacactc aagatatggc taatcacctt tttcaatgca aaacaaagag tatattttgc
       61 aaaatcaata tctataattt atctttaata cccttttaat actaaaacca aattcaaatt
      121 acatagtcat atcgtgtaat ataccaaagg tacatagaca ttcatctata tagatgataa
      181 tctatttaac gtctaggagg tatgttatga cgtataaaga actagcaaca tttttaaaag
      241 ttttatcaga ttcaagcaga ttagaaatac tagatttact ttcttgtgga gagttatgcg
      301 cttgtgattt gttagcacat tttcaattct ctcaacccac acttagctat catttgaaag
      361 cattagtaaa aaccaactta gttacgacac gaaaaatcgg aaataaacat ttataccagc
      421 ttaatcataa tatttttgag tccgtaatta ataacttgtc taaggttcat acctctaacc
      481 aacgatgttt ttgtcataac cttaagactg gtgaatgcta atgatgacaa ctttagcgac
      541 actcattttt ctagtaactt tattatttgt attatggcaa cctaaaggcc tagatattgg
      601 cattaccgca ttaactggtg cctttattgc tgttattact ggtgttgtaa gtttttccga
      661 tgttttcgaa gtaacaggta ttgtttggaa tgctactttg acttttgtct cagtcattct
      721 tatttcatta atattagata aagttggatt attcgaatgg tcagctattc acatgcttca
      781 tgcttcaaaa ggaaatggtt taaaaatgtt cgtttatatc atattattgg gtgccattgt
      841 tgctgcattt ttcgcaaatg atggcgcagc gttaatctta acgcctattg tattagcgat
      901 ggttaaaaat ataggtttta gtaagcgggc catattccct tttattattg cgagtggttt
      961 tatagctgac acaacttctt tacctttgat cgtgagcaat ctagtgaata ttatatctgc
     1021 tgattatttt catgtaggat tcgttcgata ttttagtaga atgattatac ctaatttatt
     1081 ctcgctttta gcaagtatta tagtattgtg gttatatttt agaaaggcga tacctaaaac
     1141 gtttgatgat aataatataa agcatcctaa agatgccatt aatgatttaa agctatttaa
     1201 aatttcatgg attgttctag ttatattact tttcggctat ctaatcagtg aatttactaa
     1261 aattccggta tcaattttca ctggaatcat tgcttttatt tttctaatgt tggctcgtaa
     1321 atcaaatgct gtaaatatta agcaagtcat taagggcgca ccttggaata tagtattatt
     1381 ttcaattggt atgtatatcg tcgtattcgg cttaagaaat gctggcatta ctttaatatt
     1441 ggctaaaata ttagaatata tttccaatta cggtctattt agcaccattt tgggaatggg
     1501 cttcatttca gcgtttttat catcaataat gaataatatg cctacagttt taatagatgc
     1561 gattgctatt ggtcaatcaa atgtccatgg catgttaaaa gaaggcctaa tttatgcgaa
     1621 tgttatcggt tctgatttag gtccaaaaat tacaccgata ggctctttag ctacattact
     1681 gtggttacac gtcttaacac aaaaagatgt taagatttct tggggcacat actttaaaac
     1741 tggtatcatc attacaattc cagtactatt tataaccctc atagggttgt atctaacact
     1801 tatcatattt taagaaataa aaagacgctt tctatttatt gcgatagaaa gcgtcttttt
     1861 atacttgagc aagttatatc ataacttaac tctatcaaat tacttattca aatgtttact
     1921 gtcatcttta tacacaaagt atttgaagta ttttccttca gtcttcatat tcttataaaa
     1981 gtcagcgata attgttgcat tactttctgc ccacttaata tcagtagcat attgatgttc
     2041 tcctggattt tttggattcc atctcatact atacaatgta ttttgatctg tgcttgataa
     2101 gaagtgctta tgaatgaaat cagcaccgcc tgaaatagct ttttcaggtg tatcccaacc
     2161 atgcttttta gcatattctg cacctgtttt aattgggtct ttatcaaggg ctcctactcc
     2221 atagaaattg tagtattttt tgccatcaat ttcgactcca ttagctaatt cacttttaac
     2281 tgcgccagtt tctaataatg catgtgaaat taaataaact tcgttaacgt gcttatcttt
     2341 agcagctttt aagaaatcat ccgtatgttt caataacgtt ggtctatcta ctaacatacg
     2401 tttaattcta tttttatcaa tcccttgata ctttgataaa tctaaaaatt gatatttttg
     2461 cttttcatta tcgataaaag taccgctatc cattgcactt ttaatttcag ttgcagatgc
     2521 atctctccat gcatcattct ttttatttga tacctgttga ctcgtataat tatttatttg
     2581 tttctttgct gcatcgttta atgtaacatt taacttttca atcttaatgt cggatttaac
     2641 atgtttgaaa aatatctgat cggatatcat tgagaaaaat aaaaatgaga caacagcaaa
     2701 tatgacaaca agtcctatta ttccaaaaat agaacctttc ttgtgtttat tcatatccac
     2761 acctcttagg tcattgttgt tatacattca gtttgactac gataataaag tttaacatta
     2821 ttaaaaccgt ttaacaattt tatctatgca ttgttacata ttcataaata aacagtaatg
     2881 taaatgaaac gttttaaacc atttatcaac attactttac gttgatctac gaatcataaa
     2941 tgttttatct ttttgtataa cctttgaaaa cctttatatc aataatctta ttattcacgt
     3001 attaaaaaac aaaaaattct tttgttcgtt tacttagttt cttttttata atccatcata
     3061 ataaaacttg ctgcatttga tctattttta taatagtact gatttaaatc aatcgtgcct
     3121 tcaatttgac catcacgata catcatttca ttagtgaagt tatttatcat cacgaaatca
     3181 gcgcgatctc ttataacatc taaatcgtcg tttcgtgcaa aaaaatgttc taaatttaaa
     3241 tgtgcgtaat ccattgttac ctcccgatta caaattttgt tctatctact atttagcaaa
     3301 gtaataatac atcatatcac attaaattaa ttacttatgt cttatattta acacttaatt
     3361 tagcgattac gcaaatgaat gatttttcta aatattttat tctaacttta tccggaaata
     3421 tttgttatac gtataacttg gagaatgact acttgaaatt taatgacgta tacaacaaac
     3481 accacaaaat catacaccat cttttaaaaa aatataatat tagctataat tatgatgagt
     3541 attatcaact actcttgata aaaatgtggc aattgagtca gatatataaa ccctcaagca
     3601 agcaatcttt atcctctttt ttattcactc gattaaatta ttaccttatc gatttattca
     3661 gacaacaaaa tcaattaaaa gatgtcattt tatgtgagaa taattcacca acattaactg
     3721 aacaaccaac atactttaat gaacatgacc ttcgtttaca agatatcttc aagcttttaa
     3781 atcatagaga aagactatgg ctcaaactat accttgaagg atacaagcaa tttgaaattg
     3841 ctgaaatcat gtcattatcg ctttcaacga ttaaattaat taagatgtcc gttaagcgta
     3901 aatgccaaca taattttaat tagtttattg tgaaatttcg gtcgatgtgt tagtgatgtt
     3961 gaaacaccct aacatcttca tataaattta ttttaaccat taaattaaca acttattata
     4021 cgaggagtac tatatttgca agacaattct actaaatatc tactttatat ccaaactgct
     4081 acttcaaacc atctcgaaac aaattgtgtc tttttacatt gcgattacat tcttaaagtt
     4141 ccaattaaca aactcgtttc atattatgcg aaattgcatt tatcatcaca aagtgtgcta
     4201 attgagactg caaaaaacat actaaatatt aataaactgg ttcctattta catcaaccca
     4261 aaaactatac tttttccgtt aaaacataaa cgtgcaccga tacaaattta tatcaatgca
     4321 cattatattg ttggtatgac tgccatagag aattcaacat taatacattt tcaagaaggc
     4381 attcagctag aagttgatga gccattttct ctagtttcaa aaaaatgtca tgaaagttta
     4441 gctttgaagc acttcattga aaatactatt tcgaattaaa tttaatttct aaaagtttct
     4501 tagtaaagta tgatgtcaca atttttataa aatatatgct aacaatcatt aataaaatcg
     4561 ctccaataat tgttaaaata acaacagttg ttgataaatc taccttagga ctaacgaaaa
     4621 attgtacgcc agatagactt gtaattagcc caaataaaaa caagcctaat aaagcaacat
     4681 aagcaaacat catcactact aatgtaacaa acatactata actttttgtt tctttatttc
     4741 gaaatacatt ttgtcttaag taagcattag caaaggtaac aggcttattt aagttaacgc
     4801 tgtcatcatc agatatagaa gataatcgtt gatctaatgc ttgtttctcc ttcttgttca
     4861 aaaaccataa ttgcttttta acttttttat caaatgatgc tttattcata attaaggatt
     4921 tctctccttt ataatattga cgtaatttta aacattagcg tcaattgaaa gtgtatttta
     4981 tatattttac agaaatatat gtatcaaatc tacaaaaatc catattacaa tttcatttat
     5041 ggtgatttga cgctaaaata cagtctgcta atttttcaaa ctaatgcttt ttaggtaggt
     5101 acaatgataa attttaatcg tatatatgtt tcatatataa tgaatctagc cttaaccata
     5161 ttgcattcaa aataatttta aaaaatgaaa agaaaggatt
//
LOCUS       DAOBBH010000161         5192 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.161, whole genome shotgun sequence.
ACCESSION   DAOBBH010000161 DAOBBH010000000
VERSION     DAOBBH010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.161"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(195..1130)
                     /gene="trxB"
                     /locus_tag="P6L01_002142"
     CDS             complement(195..1130)
                     /gene="trxB"
                     /locus_tag="P6L01_002142"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDP3031192.1"
                     /translation="MTEIDFDIAIIGAGPAGMTAAVYASRANLKTVMIERGIPGGQMA
                     NTEEVENFPGFEMITGPDLSTKMFEHAKKFGAVYQYGDIKSVEDKGEYKVINFGNKEL
                     TAKAVIIATGAEYKKIGVPGEQELGGRGVSYCAVCDGAFFKNKRLFVIGGGDSAVEEG
                     TFLTKFADKVTIVHRRDELRAQRILQDRAFKNDKIDFIWSHTLKSINEKDGKVGSVTL
                     TSTKDGSEETHEADGVFIYIGMKPLTAPFKDLGITNDVGYIVTKDDMTTSVPGIFAAG
                     DVRDKGLRQIVTATGDGSIAAQSAAEYIEHLNDQA"
     gene            complement(1197..2636)
                     /locus_tag="P6L01_002143"
     CDS             complement(1197..2636)
                     /locus_tag="P6L01_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDP3031193.1"
                     /translation="MAQKNNNVIPMTFDDAFYRKMAKQKFKQREYKRAAEYFEKVLEL
                     SPDDLEIQIDYAQCLVQLGIAKKAEHLFYDNIIYNRHLEDSFYELSQLNIEVNEPNKA
                     FLFGINYVIVSDDQDYRDELDQMFDVKYQSEEQIELEAQLFVVQILFQYLFSQGRLKD
                     AKNYVLHQPQEVQDHRVVRNLLAMCYLYLGEYDTAKALYEALLQEDSTDIYALCHYTL
                     LLYNTKENEQYQKYLKILNKVVPMNDDESFKLGIVLSYLKQYRASQQLLYPLYKKGKF
                     LSIQMYNALAYNYYYLGEEDESHYYWDKLKQISKVEIGHAPWVIENSKEVFDQHILPL
                     LQSDDSHYRLYGIFLLDQLNGKEIVMTESIWQVLENLNNYEKLYLTYLVQGLTLNKLD
                     FIHRGLLTLYQNELFVSENDLMVAWINQGELIIAEKVDLTDVEPYIGAFIYLYFKNQP
                     RNVTKKQITTWLGITQYKLNKMIEFLLSI"
     gene            complement(2644..3129)
                     /locus_tag="P6L01_002144"
     CDS             complement(2644..3129)
                     /locus_tag="P6L01_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDP3031194.1"
                     /translation="MRKFLSKTHHHTNPLWRVYRLVKFSKVFKNVIIIEFSKFIPSMV
                     LKRHIYKQLLNINIGNQSSIAYKVMLDIFYPELITIGSNSVIGYNVTILTHEALVDEF
                     RYGPVTIGSNTLIGANATILPGITIGDNVKVAAGTVVSKDIPDNGFAYGNPMYIKMIR
                     R"
     gene            complement(3137..3976)
                     /locus_tag="P6L01_002145"
     CDS             complement(3137..3976)
                     /locus_tag="P6L01_002145"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDP3031195.1"
                     /translation="MGIVFNYIDPVAFNLGPLSVRWYGIIIAVGILLGYFVAQRALVK
                     AGLHKDTLVDIIFYSALFGFIAARIYFVIFQWPYYAENPSEIIKIWHGGIAIHGGLIG
                     GFIAGVIVCKVKNLNPFQIGDIVAPSIILAQGIGRWGNFMNHEAHGGPVSRAFLEQLH
                     LPNFIIENMYINGQYYHPTFLYESIWDVAGFIILVNIRKHLKLGETFFLYLTWYSIGR
                     FFIEGLRTDSLMLTSNIRVAQLVSILLILISISLIVYRRIKYNPPLYSKVGALPWPTK
                     KVK"
     gene            complement(3982..4914)
                     /locus_tag="P6L01_002146"
     CDS             complement(3982..4914)
                     /locus_tag="P6L01_002146"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDP3031196.1"
                     /translation="MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSH
                     YASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKE
                     LNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSET
                     ALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSI
                     LTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVA
                     AMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE"
BASE COUNT         1678 a         1005 c          678 g         1831 t
ORIGIN      
        1 taaagttata agtttaatat aacatataaa aaaaactaga actcattgaa acgcacaaat
       61 gatacgtact aagttctagt tttctatata atttgcttta atttaaaagt aagacattat
      121 tttaacaagg tgatgacact attaaaataa atatccaaat aacttacaac tcaacatctt
      181 aattcgacct cgaattaagc ttgatcgttt aaatgttcaa tatattccgc tgcactttgc
      241 gctgcaatac taccatcgcc agtagcagtg acaatttggc gtaaaccttt gtcacgaaca
      301 tctcctgctg caaaaatacc tggtactgat gttgtcatat catcttttgt cacaatataa
      361 ccaacatcat tcgtaatacc taagtcttta aatggcgctg ttaatggttt cataccaata
      421 tagatgaata caccatcagc ctcgtgtgtt tcttctgaac catcttttgt agacgttaat
      481 gtcacagaac ccactttgcc gtctttttca ttaattgatt tcaaagtatg actccaaata
      541 aagtcgattt tatcattttt gaatgctcta tcttgtaaaa tacgctgtgc acgtaactca
      601 tcacgacggt gaacgattgt tactttgtca gcaaatttag ttaagaatgt tccctcttct
      661 actgctgagt caccaccacc gataacgaat aggcgtttat ttttaaagaa tgcaccatca
      721 catactgcac aataacttac accgcgtcca ccaagttctt gttcacccgg aacaccaatt
      781 ttcttgtatt ctgcacctgt agcaataatg accgctttcg ctgttaattc tttattacca
      841 aagttaatca ctttatattc gcctttatct tctacagatt taatatctcc atattgataa
      901 actgcaccaa actttttagc gtgttcaaac atttttgtag ataaatctgg acctgtaatc
      961 atttcgaaac cagggaagtt ctctacttct tctgtattag ccatttgacc gcctggaata
     1021 cctctttcaa tcataactgt ttttaaatta gcacgtgatg cgtatactgc agcagtcata
     1081 ccagctggac ctgcaccgat aattgctata tcaaaatcta tttcagtcat tttattaacg
     1141 cctcctcatt attaatcatt atgcgcatta tataataaat ctaacttttc ataaatctat
     1201 atgctcaaga gaaattcaat cattttgttc agtttatatt gtgttatgcc taaccatgtt
     1261 gtaatttgct tctttgtaac gtttcgaggt tgatttttaa aatacaaata aataaacgca
     1321 ccgatatatg gctcaacatc agttaaatct actttttcag caattatgag ttcaccttga
     1381 ttaatccatg caaccattaa atcattttca cttacaaata attcattttg gtaaagcgtt
     1441 aacaagccgc gatgaatgaa gtctaattta ttgagcgtta aaccttgaac taaatacgtt
     1501 aaatacaatt tctcataatt atttagattt tccagaacct gccaaatact ttccgtcatc
     1561 acaatttctt taccatttaa ttgatccaat aaaaaaatac catataaacg ataatgactg
     1621 tcatcacttt gaagtaatgg caaaatatgt tggtcaaaaa cttctttgct attttcaatt
     1681 acccaaggcg catgtccaat ttccacttta gaaatttgct tcaatttatc ccagtagtaa
     1741 tgactttcgt cttcttcacc taaataataa taattatatg ctaaagcatt gtacatttga
     1801 attgataaaa atttcccttt tttatataaa gggtacaaca attgttgtga tgcacgatac
     1861 tgctttaaat aacttaatac aatacctaat ttaaaacttt catcgtcatt cataggtaca
     1921 actttgttta atatttttaa atatttttga tattgttcat tttccttagt gttataaagt
     1981 agcaaagtat aatggcataa tgcatatata tctgtactat cctcttgtaa tagtgcttcg
     2041 tacaatgctt tagccgtatc atattcaccg agatataaat aacacattgc caataaatta
     2101 cgtacgacac gatgatcttg aacttcttgt ggttgatgta agacataatt ctttgcatct
     2161 tttaatcgac cttgagaaaa aagatattgg aatagtattt gaactacaaa caattgagct
     2221 tcaagttcaa tttgttcttc gctttgatat ttcacatcaa acatttgatc taattcatct
     2281 ctataatctt ggtcgtcgct aacaataaca taattaatac caaacaagaa tgccttgttt
     2341 ggttcgttaa cttcaatgtt gagctgactc aattcataaa agctatcttc tagatgccta
     2401 ttataaataa tattgtcata aaataaatgt tctgcttttt tagcaatacc aagttgcact
     2461 agacattgtg cataatcaat ttgaatttcc agatcatcag gtgacaattc taacactttt
     2521 tcaaagtatt cagcagctcg tttatattct ctttgtttaa acttctgttt agccatttta
     2581 cgataaaatg catcatcaaa agtcattgga attacattat tattcttttg cgccataaaa
     2641 ttgtcacctc ctaatcattt ttatatacat agggttgcca tatgcaaatc cattatccgg
     2701 tatatctttt gaaacaaccg taccagctgc aacttttaca ttgtcaccaa tcgttatacc
     2761 gggtaaaatg gtagcatttg caccaatcaa agtgttagat cctatcgtca ctggtccata
     2821 acgaaattca tcaactaatg cttcatgcgt caaaattgtt acattgtaac caataacact
     2881 gttactacca atcgtaatca gttctgggta aaaaatatct aacattactt tataagctat
     2941 cgatgattga ttaccgatat taatatttaa aagttgttta tatatatgtc ttttcagtac
     3001 catacttgga ataaatttcg aaaattcaat gataattaca ttcttaaaaa ctttcgaaaa
     3061 tttaacaaga cggtatacac gccataaagg gtttgtatga tgatgtgttt ttgataaaaa
     3121 ttttctcaaa atatcactac ttcacttttt ttgttggcca tggaagcgcc ccaactttgc
     3181 tatacaacgg tggattatac ttaatccttc tatatacaat taaacttata cttattaaaa
     3241 ttaaaagaat tgatactaat tgtgcaactc taatattact tgtgagcatt aagctatctg
     3301 tacgtaatcc ttctataaag aatcgaccaa ttgaatacca agttaaatat aaaaagaatg
     3361 tttctcctaa ttttaaatgt ttacgaatat taactaagat aataaatcca gcgacatccc
     3421 aaatggattc atataagaat gttggatgat aatattggcc gttaatatac atattttcta
     3481 ttataaaatt aggcaaatgt aattgttcta aaaaagcgcg tgacaccggt ccaccatgtg
     3541 cctcgtgatt cataaagtta ccccagcgtc caattccttg cgctaaaatt atacttggcg
     3601 caacgatatc accaatttga aatgggttta aatttttcac tttacataca ataacaccag
     3661 caataaagcc acctattaaa ccaccatgta ttgctattcc accatgccat attttaataa
     3721 tttcacttgg attttccgcg taatatggcc attggaaaat cacaaaatag attcgtgccg
     3781 cgataaatcc aaatagtgca ctataaaaaa taatatctac taaagtatct ttatgtaatc
     3841 ctgctttaac tagtgcacgt tgtgcaacaa agtaaccaag taatattccg acagcaatga
     3901 taattccata ccatcgtaca ctcagtggtc ctaagttaaa tgccacagga tctatatagt
     3961 taaatacaat acccattcaa cctactcctc actcttatga ctgttcttga taatttcttc
     4021 atttaatctt tcactaaatt cttcggcagt gttaatgccc atgatattta atcgatagtt
     4081 cattgcagcg acctcaataa ttaccgcaac atttctacca ggtcttacag gtattgtttt
     4141 tttagtgatt tcagtatcta aaatacttag cgtctcttca ttaagaccta cgcggtcata
     4201 taacttttgc ttgttccagt tttccaaatt aatatttaat ctaattcgtt tttcagttaa
     4261 tattgaaccc gcgccaaata aagtcataac attgataata cctagtccac gtatttctaa
     4321 tagatgttct attaactttg gtggtttccc tattagttca tctttattaa tttgacgtat
     4381 ttctacatta tcatctgcta ctaatctatg cccacgttta actaattcca acgcagtctc
     4441 acttttacct attcctgaat caccggtaat tagtacacca acaccgtaaa catctactaa
     4501 aacaccatgt aaagatgtcg tctttgcaag tgcatgctct aaaaacgttg ttaagcgact
     4561 cattaaactt gtagtcgcat ctttagcaac tataagtggg gtatttaatt cttttgcagc
     4621 ttcaactaat tcttctggtg gctgcaatcc acgtgtcaca ataattgcag gcgtttctgg
     4681 tctgcatagt ttacgcatac gacctgcgcg atccttatct ggtaataaat tgtaaaacga
     4741 tagttccgtt gttcctaata gttgtattct atctgacgca taatgtgaaa aataacctgc
     4801 catctctaag cctggtcttg atatatcagc atttttaatt ggcttcgata gtccttcttc
     4861 accagcgatt aaatctaact ttaatgtttc aactagtttt tctgtcgtta acatgggttc
     4921 acctcaattg tatttatccc tactcttaca tctcttctta tcatatcaaa aatataacac
     4981 caatctacat tgaaaagcta aaataaatat taattgttca ttattgttat tattttacaa
     5041 gtcaatatca atcattaatt tattgcttgc ttaatttaca tggttcatat cgccataaaa
     5101 gtccgagcca taaatcaatg acctaagctc tacaatgata tattgtaatt agttggcggg
     5161 gccccaacat aaagaatttc gaaaagaaat tc
//
LOCUS       DAOBBH010000162         5145 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.162, whole genome shotgun sequence.
ACCESSION   DAOBBH010000162 DAOBBH010000000
VERSION     DAOBBH010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.162"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..3407)
                     /locus_tag="P6L01_002147"
     CDS             complement(<1..3407)
                     /locus_tag="P6L01_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HDP3031197.1"
                     /translation="MINKEQLDLLYKLKKEVEKSRNEALLHTINQVIKKVYLQQYTCS
                     FVGHFSAGKSTLINLLIEQDILPSSPVPTTSNTAIVSVSDNHDIIANLPNQTYAKLSN
                     YDEVREMNRQNVDVESVEINFQSAKFENGFTLQDTPGVDSNVASHQSITEQYMYTSNM
                     IFYTVDYNHVQSELNFKFMKHINDVGIPVVFIINQIDKHQDDELSFSTFKSRVEKSIA
                     DWGIKLERTFYVSKFDHPENELEVLSSYLISLDQHRETIEDYTSRTVEYITEAQLDYI
                     QSEIQEVLEDLGIEEAEFEQAFLNSQQHQAISEEAQLLNNPDELMAFLKNKRKNILEN
                     AYIMPHNMREMLRSYLESMSQDFNVGGFFNKKKKKLQIQQQRLLTATDALQEHVNQQI
                     RQPMREDMSFVTRFINKKEASDKVLNQHYDVKPEMIEDLYQPQTSISNTYVLTFSDEV
                     VKAIKKYVEQQSTPIFKEIIENVQADELPTEESDDLKEYQRYTELNELRQSLTTKNYR
                     HYYIHLDESLDKLIGRQETTYQVATDNTQDNRDNQQLNQNTATTNMSIDIQKALDIIS
                     DVPLFKRTKQDIHETLTRIDNKLIKIGVFGTFSAGKSSLINALLGEHILVSSPNPTTA
                     ATTEISYGDESYITLKSQSQLLDEINAVVEYQDISFSTIEEFINWDLEKLKSHLNKNQ
                     LAFIQAVEKHYKLYVNMLENGEKHAINQQELKKWSAEDEYATFVKTVHIALMHDWLKG
                     KIIVDSLGLHSNNQRHTNETEQILTSSDLILYVSYFNHSFTDNDKAFIEHMKDMNQLN
                     ENQAFKMVINAADLAESQDDLEAVETYVSDALRQVHLQSDIFAVSSRNALQAEDKGID
                     QLKQSIQQFVDVESKSILEQQMIHQLQQMDRSYVEMITEFETNKADISRRQQRLTDYK
                     DKQRLQHQLIDATLQHTDNEVEEQVYHLNARLKLQLLDDVKSVFNSQMTQNSDFNEEK
                     KVSTKVYLDQIHQRLFLEQSLITERIKKYFNKQLTKQIAPIVQQLADLHVIVNPQFNF
                     ESANIEQPLLHIDFNDMLNALPKQLTKRKILNPNGQRDIHESICQSTLGLLQPQMGLL
                     RQQLELYVKQMAVEAESQFESFEANIQTQINDLLAFDLDTTLINQLK"
     gene            3711..5051
                     /locus_tag="P6L01_002148"
     CDS             3711..5051
                     /locus_tag="P6L01_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDP3031198.1"
                     /translation="MKNTFNPLQRLHFYAAIFIAPLLITLTISGIGYLFFPEVENNIY
                     KNEFFGDSDVKTHQTLNDAVHQVEQQYEGFFVSKVSILDEPYNKRITLSDMAGNQRYV
                     FLDHNNQFVADQNAKHTYSNVMRSIHSSLFTENTIINYLVELTACWTIFMILSGTYLL
                     IKKHLISNKSKALRWQKWHAMIGVIIAIPVFVLVLTGLPWSGFMGSKIAGMMDTNGDL
                     GQGELAINPPKSDLNELPWATRKNKQPASSEKGSSGHHGNAAMPQTKLDYQISIDKVV
                     VQAQKAGIKKPFSIVYPSDKNGTFIVSNTSNSGVTGLDVSPYKEQTLYFDQYSGKKLG
                     TIKYDDYGIIAKWFTWGIPLHEGHLFGILNKIINLFVCITLLVAIGMGFVSWIKRTKN
                     TAVKVPHRVKKPASISLIICLIVLGLLMPLFGLSLILVFIIELILYIKDRRAKQ"
BASE COUNT         1608 a          824 c          773 g         1940 t
ORIGIN      
        1 tctttcaatt gattgataag tgttgtatct aaatcaaatg ctaataaatc gtttatttgc
       61 gtttgaatat tagcttcaaa actttcaaat tgcgattcag cttctacagc catttgcttt
      121 acatataatt caagctgttg cctcaataat cccatttgtg gttgtaataa tcctaacgta
      181 ctttgacaaa ttgattcatg tatatctctt tgcccatttg gattcaaaat tttacgtttt
      241 gttaattgtt taggcaatgc atttagcata tcattgaaat cgatgtgcaa taatggttgc
      301 tctatattag ctgattcaaa gttaaactga ggattaacaa tgacatgtaa atctgctaat
      361 tgttgaacga ttggtgcaat ttgcttagtg agttgcttat taaagtattt ttttatacgt
      421 tctgtaatta aagattgctc taaaaacaat cgttgatgaa tttgatctaa gtatactttc
      481 gtagacactt tcttttcttc attaaaatca ctattttgcg tcatttgaga attaaacact
      541 gatttaacat cgtcaagtag ttgtaatttt aaacgtgcat ttaaatgata aacttgttct
      601 tcaacttcgt tgtctgtatg ttgtagcgtc gcatcaatta attgatgttg taaacgctgt
      661 ttatctttat aatctgttaa cctttgttgg cgacgtgaaa tatcagcttt atttgtttca
      721 aattctgtaa tcatctctac ataagaacga tccatttgtt gaagctgatg aatcatttgt
      781 tgttctaaaa ttgatttaga ttcaacatca acaaattgtt gtatgctttg ttttaattga
      841 tcaatgccct tatcttcagc ttgcaatgca tttcgacttg atacagcaaa aatgtctgat
      901 tgtaagtgta cttgtcttaa tgcatctgat acatatgttt caactgcttc aagatcatct
      961 tgactttctg ctaaatcagc agcattaatt accattttaa atgcttggtt ttcattcaac
     1021 tggttcatat ctttcatgtg ttctataaac gctttgtcat tatcagtaaa tgaatgatta
     1081 aaataactta catacaatat taagtctgaa gaagttaaaa tttgttcggt ttcatttgta
     1141 tgcctttggt tatttgagtg tagccctaat gaatcaacaa ttattttacc ttttaaccaa
     1201 tcatgcatta atgcaatgtg tactgtctta acaaatgttg catattcatc ttctgcactc
     1261 cactttttca attcctgttg attaatggca tgtttttctc cattttccaa catattgaca
     1321 tacaatttat aatgtttttc aactgcctga ataaaagcga gttgattttt atttaaatgt
     1381 gattttaact tttctaaatc ccaattaata aattcttcta tagttgaaaa agatatatct
     1441 tggtattcaa ctactgcatt aatttcatct aataattgcg attgtgattt tagcgttata
     1501 taactctcgt ctccataaga tatttctgta gtagctgctg tagtaggatt tggagaactc
     1561 actaaaatat gctcgcctaa taatgcattt atcaaactac ttttacccgc actaaatgtt
     1621 ccaaatacac ctatttttat taatttatta tctatacgtg ttaatgtttc gtggatatct
     1681 tgctttgtac gcttgaacaa aggcacatcc gaaattatat caagcgcttt ttgaatatct
     1741 atagacatat ttgttgtagc tgtattttga tttagctgct gattatcacg attatcctga
     1801 gtattatcag tagccacttg atatgttgtc tcttgtcgac ctattaattt atctagagat
     1861 tcatctaaat gaatatagta gtgacgataa ttcttagtcg tcaatgactg acgcagctca
     1921 tttaattctg tataacgttg atattctttt aaatcatcac tttcttctgt tggtaattca
     1981 tctgcctgca cattttctat tatttcttta aaaatcggtg ttgattgttg ttcaacatat
     2041 ttcttaatgg ctttaaccac ttcgtctgaa aatgtaagta cataagtatt gctgattgat
     2101 gtttgtggtt gatataaatc ttcaatcatt tccggtttaa cgtcataatg ctgatttaat
     2161 actttatctg aagcttcttt tttattgata aaacgcgtta caaatgacat atcttctcgc
     2221 attggttgac gaatttgttg attaacatgt tcttgtaacg catctgtcgc tgttaataat
     2281 cgctgttgtt gaatttgtag ctttttcttc tttttattaa aaaacccacc aacattaaaa
     2341 tcttgagaca tactttccaa ataacttcgt aacatttctc tcatattatg cggcataatg
     2401 tatgcatttt ctaaaatatt tttacgctta ttctttaaaa atgccattaa ttcatctgga
     2461 ttatttaaaa gttgtgcctc ttcactaatt gcttgatgtt gttgactatt taaaaatgct
     2521 tgttcaaact ccgcttcttc gatacctaaa tcttctagta cttcctgaat ttcagactga
     2581 atgtaatcta gctgagcttc ggtaatgtat tcaaccgttc ttgatgtata atcctctatt
     2641 gtctctctat gttgatctaa tgaaattaga taacttgata atacttcaag ttcattttca
     2701 gggtgatcaa atttagatac ataaaaggtg cgttctaatt taatgcccca atctgcaatt
     2761 gatttttcaa ctcgtgattt aaacgtagag aatgacaatt catcgtcttg atgcttgtca
     2821 atttgattaa tgataaacac aacaggtatt ccaacatcat ttatatgctt cataaactta
     2881 aagttaagtt cagattgaac gtggttatag tcaaccgtat aaaatatcat attacttgta
     2941 tacatatatt gttctgttat tgactgatgt gatgcaacat ttgaatcaac acctggtgta
     3001 tcttgcaacg taaacccatt ttcaaattta gctgattgaa aattaatttc tacagattca
     3061 acgtcgacat tttggcgatt catttccctt acttcatcat aattagataa tttggcatat
     3121 gtttgattcg gcaaattagc aataatatcg tgattgtctg aaactgacac aatagcagta
     3181 ttacttgtcg ttggtacagg tgaacttggt aagatatctt gttcaattaa taaatttatc
     3241 agtgtcgatt tacctgcaga aaaatgtcca acgaacgaac atgtatattg ctgcaaatat
     3301 actttcttaa ttacttggtt aattgtatgt aaaagtgctt catttcgcga cttttcaact
     3361 tcttttttta atttatataa aagatctaat tgttctttat taatcatgtt actttcccct
     3421 ttggttcgtt cacatagaaa tgcttgcttg ttaaaacgaa ttcatacaat atcgatgtcc
     3481 atttaaacat agatcgttaa atggctcgca ttgtattatg tttaaatttt atcatagctg
     3541 cttgataaaa catacgaata atcaattttc ttcaaacatt ccaacttaaa agttcatatt
     3601 tctaaaaatc acaaatcatc gttaaatttt tctagaaaat aaatattgtc acagtttgtt
     3661 cacacacgtc acagaattga caccgtataa tagccaattg ggagggaaat atgaaaaata
     3721 catttaatcc attacaaaga ttacatttct atgcagcaat atttattgct ccactgttaa
     3781 tcactttaac catttcgggc attggttact tgttctttcc agaagttgaa aataatattt
     3841 ataagaatga gttttttggt gacagtgatg taaaaacgca tcaaacatta aatgatgcag
     3901 tacatcaagt tgaacaacaa tatgaaggat tctttgtaag taaagttagc atacttgatg
     3961 aaccatataa caaacgaatt acactgagtg atatggcagg aaatcaacgt tacgtctttc
     4021 tagatcataa caatcaattt gttgcagatc aaaatgcgaa acatacgtat tctaatgtga
     4081 tgcgaagtat acatagttct ttgtttactg aaaatactat tattaattat ttagtagagt
     4141 taaccgcatg ttggacgata ttcatgattt tatctggtac ttatttactc attaagaagc
     4201 atttaatttc taacaaaagt aaggcacttc gttggcaaaa gtggcacgca atgattggag
     4261 ttatcattgc aattccagta tttgtattag ttttaactgg attgccatgg tctggtttta
     4321 tgggcagtaa aattgccggt atgatggaca caaacggtga ccttggtcaa ggtgaattag
     4381 cgattaatcc acctaaatca gatttgaacg aattaccttg ggctacacgt aaaaataaac
     4441 agccagcttc atccgaaaaa ggttcaagtg gtcatcatgg taatgcagca atgcctcaaa
     4501 ccaaattaga ttatcaaata tctattgata aggtcgttgt acaagcgcaa aaagctggta
     4561 ttaaaaagcc gttttcaatc gtatatccaa gtgataaaaa tggtaccttt attgtatcta
     4621 atactagtaa ttcaggtgtt actgggctag atgtatcacc ttacaaggaa caaacacttt
     4681 atttcgacca atatagcggt aaaaagctag gtacgattaa atatgatgac tacggtatta
     4741 ttgctaaatg gtttacatgg ggcattccgc ttcacgaagg tcatttattc ggcattttaa
     4801 ataaaatcat taatttattt gtatgtatca ctttattagt agccattggc atggggtttg
     4861 tctcttggat aaagcgtaca aaaaatactg cagtaaaagt accacatcgc gtaaaaaaac
     4921 cagcatctat atcactcata atatgtttaa ttgtattagg attattaatg ccattatttg
     4981 gattatcact tatccttgta tttataattg aattaatatt atatattaaa gatcgtcgtg
     5041 ctaaacaata atgcacttaa agttttgaac tgacgaaatt tacaaaatga ttctcgtctc
     5101 tctaattact taaaacgggt tctataataa atcgtactga tggaa
//
LOCUS       DAOBBH010000163         5139 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.163, whole genome shotgun sequence.
ACCESSION   DAOBBH010000163 DAOBBH010000000
VERSION     DAOBBH010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5139
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.163"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..160
                     /locus_tag="P6L01_002149"
     CDS             <1..160
                     /locus_tag="P6L01_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDP3031199.1"
                     /translation="KYINMTIPKGAGAKVDVIAAKIIKKYGLSRLDTISKKHFKNREK
                     AQKILKPL"
     gene            complement(592..2994)
                     /locus_tag="P6L01_002150"
     CDS             complement(592..2994)
                     /locus_tag="P6L01_002150"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDP3031200.1"
                     /translation="MLISNEWLKEYVTIDDSVSDLAERITRTGIEVDDLIDYTKDIKN
                     LVVGFVKSKEKHPDADKLNVCQVDIGEDEPVQIVCGAPNVDAGQYVIVAKVGGRLPGG
                     IKIKRAKLRGERSEGMICSLQEIGISSNYIPKSFESGIYVFSESQVPGTDALQALYLD
                     DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPETTSNELELSANDELTVTIENE
                     DKVPYYSARVVHDVTIEPSPIWMQARLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQ
                     DAIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIALAGVMGGDFSEVKE
                     QTSNIVIEGAIFDPVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYA
                     NGKVLKDRVSSGELGAFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDV
                     ITVLVPSRRKDITIKEDLIEEVARIYGYDDIPSTLPVFDKVTSGQLTDRQYKTRMVKE
                     VLEGAGLDQAITYSLVSKEDATAFSMQQRQTIDLLMPMSEAHASLRQSLLPHLIEAAS
                     YNVARKNKDVKLFEIGNVFFANGEGELPDQVEYLSGILTGDYVVNQWQGKKETVDFYL
                     AKGVVDRVSEKLNLEFSYRRADIDGLHPGRTAEILLENKVVGFIGELHPTLAADNDLK
                     RTYVFELNFDALMAVSVGYINYQPIPRFPGMSRDIALEVDQNIPAADLLSTIHAHGGN
                     ILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAV
                     IR"
     gene            complement(2994..4052)
                     /gene="pheS"
                     /locus_tag="P6L01_002151"
     CDS             complement(2994..4052)
                     /gene="pheS"
                     /locus_tag="P6L01_002151"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDP3031201.1"
                     /translation="MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGL
                     MKLMKDLPNEEKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGR
                     HIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDS
                     FYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGL
                     VVDKNVKMSDLKGTLELLAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCN
                     VCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYT
                     NDVRFLDQFKAVEDRGDM"
     gene            complement(4432..>5139)
                     /locus_tag="P6L01_002152"
     CDS             complement(4432..>5139)
                     /locus_tag="P6L01_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDP3031202.1"
                     /translation="IKQANKLKKKRERDKTGLALIEGVHLIEEAYQSGIVITQLFAIE
                     PARLDQQIIAYAQEVFEINMKVAESLSGTVTPQGFFAIIEKPHYDISKAQQVLLIDRV
                     QDPGNLGTLIRTADAAGMDAVIMEKGTTDPYQDKVLRASQGSVFHLPVMTQDLDTFIT
                     QFNGPVYGTALENAVAYKEVTSSDSFALLLGNEGEGVNPELLAHTTQNLIIPIYGKAE
                     SLNVAIAGSILLYHLKG"
BASE COUNT         1595 a         1031 c          734 g         1779 t
ORIGIN      
        1 aaaatatatt aatatgacga ttcctaaagg tgccggtgct aaagtcgatg taattgccgc
       61 aaaaattata aaaaaatacg gtctttcacg tttagatacc atttcaaaaa agcattttaa
      121 aaatcgtgaa aaagcccaaa aaattttaaa gcctctttga tttttaatca ttagaggctt
      181 ttatgcagtt gtgcgaagca tcatctgtat tccgctgttc ctctttgatt tataaaatga
      241 attggatcct aataggtgat cctattaggt aagtcatact aatttgcttt gctcataagt
      301 atgactttat atgcagtgat gcgaagcatc atctgtaatc cactgtgcct ctatgattta
      361 taaaatcaag ataaattgga tcctaatagg aaatcctatt aggtaagtca tactaatttg
      421 ctttgctcat aagtatgact ttatatgcag ttgtgagaag catcatctgt attccgctgt
      481 gcctctttga tttttaaatg aatcgaaacc taattggtga atccaaaaat aaaaccccat
      541 gttaaggata tcaatctact tcagatatcc ttatacatgg ggtttaaatc attatctaat
      601 aacagcacct tgttcaatta atgctgcttc aatttccgct tgcacttttg aaacgcgctc
      661 atctgtcaat gtttcttctg tgtctaaata atttaaacgt attgcaattg attttttacc
      721 tttttctaaa tgttcgccct gatatacatc aaagacaagt gtatctttta atatattgcc
      781 accgtgtgca tgaatcgttg ataataaatc agctgctgga atattttgat ctacttctaa
      841 tgcaatgtca cgagacatgc ctgggaatct cggaattggc tggtaattaa tgtaacctac
      901 cgacacagcc attaatgcat caaaattcaa ctcaaaaaca tacgtacgtt ttaaatcatt
      961 atcagctgct aatgttggat gtaattcacc aataaaacca acaactttat tctctaataa
     1021 gatttcagca gtacgacctg gatgtaatcc atcaatatca gcacgacgat aactaaattc
     1081 aaggtttaac ttttcagata ctcgatccac gacacctttt gctaaataga aatcaaccgt
     1141 ttctttctta ccttgccatt gattgactac ataatctcca gttaaaatac cacttaaata
     1201 ttcaacttga tctggtagtt caccttctcc attagcaaag aagacattgc cgatttcaaa
     1261 taattttaca tctttatttt tgcgtgccac attatatgat gctgcttcga ttaaatgtgg
     1321 taataaactt tgacgtaatg acgcatgcgc ttcactcatt ggcatcaata aatcaattgt
     1381 ttgacgctgt tgcatcgaaa atgcagtagc atcctcttta gaaactaacg aatacgtaat
     1441 agcttgatct aatccagcac cttctaacac ttctttaacc attctagttt tatattggcg
     1501 atcagttagc tgaccactag taactttatc gaagacaggt aacgttgatg gaatatcgtc
     1561 gtatccatat atacgtgcaa cttcttcaat taaatcttct ttaattgtaa tatctttacg
     1621 acgtgatggt actagcactg taataacatc atcatttatt tctgtatcaa accctagttg
     1681 attaaaaata gtaacaatat cattttgtga caaatcaaat ccaatagtgc gattaatttt
     1741 atcagcagtg atgtcgattg gtgtaataaa tgcaccaagt tctcctgaag acactctatc
     1801 ttttagcact tttccgtttg cataagtttg taataaataa catgcacggt cgactgcttc
     1861 atctacaaat tcagtagcta ttcctttttc aaaacgacta gatgattcac tgcgtaaatt
     1921 taaacgtctt gatgtatgac gaattgaaac tggatcaaaa atagcacctt caatcactat
     1981 atttgatgtt tgttctttaa cttctgaaaa atcgccaccc ataacaccag ctaatgcaat
     2041 tggagtttgt ccattagtaa tgacaatatc gctcgttaat aattcacgtt ctgtatcatc
     2101 taatgttgtc attttttcgc cttcattagc ttgacgaaca acaatttgtt gtgaaccaat
     2161 cgcatcttga tcaaacatgt gcaatggttg accgtattct aataacacat aatttgaaat
     2221 gtcaacaaca ttattaatag gacgtatacc cgcttttatt aagcgtgctt gcatccaaat
     2281 tggcgaaggt tcaattgtca cgtcgtgaac aacacgtgca ctataatatg gtactttatc
     2341 ttcattttca attgtcacag tcaattcatc atttgcagat aactcaagct catttgatgt
     2401 tgtctctggc ttagtcattt ttgtattata taatgctgca acttcataag cagtacctat
     2461 catacttaaa gcatctgcac gattcggcgt taaatcaaat tccattactt gatcatctaa
     2521 atataaagct tgtaaggcat ctgttcctgg aacttgggat tcactaaaaa cataaatgcc
     2581 tgattcaaaa cttttcggta tatagttact tgaaatacca atttcttgta acgaacaaat
     2641 cataccttct gaacgttcac cgcgtaattt ggcacgctta attttaatac caccaggcaa
     2701 tctgccacct actttagcaa caatgacata ttgtcctgca tcaacgttcg gtgcaccaca
     2761 tacgatttgt acaggttcgt cttctccgat atcaacttgg caaacattta atttatcggc
     2821 atcaggatgt ttctcttttg acttaacgaa accgacaact aaatttttga tatcttttgt
     2881 gtagtcaatt aaatcatcca cttcaatacc tgtgcgcgta atacgttctg ccaaatcact
     2941 tacagaatca tcgattgtta catattcttt caaccattca tttgatatca acattacatg
     3001 tcacctctat cttctaccgc tttaaattga tctaaaaatc tcacatcatt agtatagaaa
     3061 tgacgaatat cttctatacc atatttcaac attgcaatac ggtctggtcc cataccaaat
     3121 gcaaatccag agtactctga agaatcaaaa ccagccattt ctaatacatt aggatgtacc
     3181 attccagcac ctaaaatttc aatccatcct gtgtgtttac acacattaca accttttcct
     3241 ttacatttaa aacatgacac atcaacttct acagaaggtt cagtgaatgg gaagtaactt
     3301 ggacgtaaac gaatttcacg atcagcacca aataatttct tagctaacaa ttctaaagtg
     3361 cctttcaaat cactcatttt aacgttttta tcaacaacta atccttcgat ttgtgtaaat
     3421 tgatgactat gtgtcgcatc atcagagtca cgacgataca ctttaccagg gcaaataatt
     3481 ttaactggac cttgaccatg acgtgattcc atcgtacgtg cctgcactgg tgatgtatgc
     3541 gtacgtaata aaatttcatc cgtaatatag aaactatctt gcatatcacg tgcagggtgt
     3601 gatttaggta aattcagcat ttcgaagtta taatgatctt gttcaacttc atatccattc
     3661 acaatttcat aacctaaacc taagaataag tcttcaattt cttctattgt acgtgttaat
     3721 ggatgctttg aaccgatttc aatatgacga cctggtaatg atacatcaat tgtttcttca
     3781 gccaattgct tatttaattt ttctttaact aacatctgtt gtctttcatc taattcattt
     3841 tgaattgttt gacgcaattc attcactttt tgaccaaacg caggtttctc ttcattcggc
     3901 aaatccttca tcaatttcat tagtccgcta actgaccctt ttttacctaa gtatttcact
     3961 ttaacttctt gcagtgcacg ttcatcattt gcttcattaa tatctacaag cgcttgttgt
     4021 tttaactctg acattgtttg ttgttcagac atatgtcact gacctcccta ttcgaactat
     4081 tcattttcta ttgatcatat ttgttgttta actaaaaaat gactttcatc ctcttaaaaa
     4141 taagggacga aagtcatttc cgtggtacca cccaaattta tgcctaagca tacacttgtt
     4201 taatattgat aacggattgt ctttccgact taaatctctt taagtaacca aaaaggtgaa
     4261 aagactttaa ataattggaa taacttgcag tcacgattat tctccctaaa acaattattt
     4321 atataaatgt ccatgccttt ttattggttt atattcaatt gtgtatgcat tcacgtagaa
     4381 atgttctgtt aaacaattaa ttataattat atcggaaaac tttcaacacg gtcaaccttt
     4441 caaatgataa agtaaaatac tacctgcaat cgctacattt aaactttcag ctttaccata
     4501 aataggtatg attaaatttt gtgtagtatg tgctaataat tcaggattaa caccttctcc
     4561 ctcattacct aatagtaatg caaaagaatc acttgaagta acttctttgt atgccactgc
     4621 gttttcaagt gctgtaccat aaacaggacc attaaattga gtaataaacg tatcgagatc
     4681 ttgtgtcata actggcaaat ggaaaacact accttgactc gctcgcaaca ctttatcttg
     4741 ataaggatct gtcgtaccct tctccattat tacagcatcc attccagcag catccgcagt
     4801 tctaattaat gtgcctaaat ttccaggatc ttgaacacga tcgatgagca atacttgttg
     4861 tgctttagaa atatcataat gcggcttctc aatgattgcg aaaaaccctt gtggtgtcac
     4921 tgtacctgat aaagattcag caactttcat gtttatttca aaaacttctt gcgcgtatgc
     4981 gataatttgc tgatctaatc ttgccggttc aattgcaaat aattgtgtaa ttacaattcc
     5041 actttgataa gcttcttcaa ttaaatgcac accttcaatt aaagctaatc cagttttatc
     5101 cctctcacgt ttctttttta gcttgttcgc ttgtttaat
//
LOCUS       DAOBBH010000164         5128 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.164, whole genome shotgun sequence.
ACCESSION   DAOBBH010000164 DAOBBH010000000
VERSION     DAOBBH010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.164"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..398
                     /locus_tag="P6L01_002153"
     CDS             <1..398
                     /locus_tag="P6L01_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HDP3031203.1"
                     /translation="KKFKYKNFEDEIEIDFTDSNHLAAHSNQSNQSMNNNDLTASKAN
                     DNSDVSTNDYHDIKSPMVGTFFLQDSKELTEPIVNVGDKVNKGDIIGYVEAMKVLNEV
                     TTDVAGEITEIVADHGTNVEYDQVLVRIK"
     gene            412..1773
                     /locus_tag="P6L01_002154"
     CDS             412..1773
                     /locus_tag="P6L01_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDP3031204.1"
                     /translation="MLRCLIANRGEIAVRIIRACREYGIETVAVYAKGDEQSLHVHLA
                     DQAICIGEANALDSYLNIDRIISAAQITGANAIHPGYGFLSESTKFAQTVEEQGIAFI
                     GPTKKTMEMMGDKITARQTVHHAGVPVIPGSNGAVNHVSEIENLAKDIGYPVVIKAAS
                     GGGGKGIRIVKKAEDLEKAFKEAKSEGKKYFDDDRVYVEAFIPVAKHVEVQVMGDGQD
                     NYVHLGERDCSVQRKNQKLIEESPCAALTEERRQQICNDAVKVARAANYRSAGTIEFL
                     VTDTAHYFIEMNARIQVEHTVTEMRAERDLVAAQLYLLEHNHLPFSQSDIQFNGHVIE
                     ARINAENPEKKFQPTPGKVTALHLPQGFNVRVDSLLYHGYQVSPYYDSLVAKVIVKSH
                     DRASAIDKLKVTLDEMVIDGFSTTADFLYAVLNYPLYRDGDAKDVDIKFLEKHQIVKG
                     VEL"
     gene            1773..2525
                     /gene="pxpA"
                     /locus_tag="P6L01_002155"
     CDS             1773..2525
                     /gene="pxpA"
                     /locus_tag="P6L01_002155"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDP3031205.1"
                     /translation="MRVDLNCDLGEAFGNYSFGGDHQIIPLITSANVACGFHAGDENV
                     MNETVKLAKAHNVAVGAHPGLPDLKGFGRRNIDISNDEIYNLMIYQLGALQGFCRIHQ
                     LKINHVKPHGALYQMGAKDREIANVIAQAVYDFDPSLVLVGLANSYLISEAKNVGLIT
                     ASEVFADRRYEDDGQLVSRKESDAVITDTDEALKQVLKMVKENKVISKNNKEVTLQAD
                     TICVHGDGEHALLFVSKIREILMKEGIDIQSL"
     gene            2538..3767
                     /locus_tag="P6L01_002156"
     CDS             2538..3767
                     /locus_tag="P6L01_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDP3031206.1"
                     /translation="MGRNLKLKKESDFEFTKNHKRLLLGSVFLMATSAIGPAFLTQTA
                     VFTSQFFASFAFAILLSIIIDIGAQINIWRILVVTGLRGQEISNKVVPGLGTVISILI
                     AFGGLAFNIGNIAGAGLGLNAIFGLDVKWGAAITAIFAILIFVSKSGQKIMDVVSMIL
                     GIVMILVVAYVMFVSNPPYGDAFVHTFAPEHPMKLVLPIITLVGGTVGGYITFAGAHR
                     ILDSGIKGKQYLPFVNQSAIAGILTTGIMRTLLFLAVLGVVVTGVTLSSENPPASVFE
                     HAIGPIGKNIFGIVLFAAAMSSVIGSAYTSATFLKTLHKSLNERSNLIVIVFIVISTM
                     IFLFIGKPISLLIIAGAINGWILPITLGAILIASKKKSIVGDYKHPNWMFIFGIVAVL
                     VTILTGIFSFKEVLQLF"
     gene            4056..>5128
                     /locus_tag="P6L01_002157"
     CDS             4056..>5128
                     /locus_tag="P6L01_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDP3031207.1"
                     /translation="MGYRTLKSIFHEHNESKMKEEYTKRFNSLASFNTNINIIPMENG
                     KKVNDLEYPLFFMVTKNLSKKQELISINSRKIDRALNSLPYAAREQYFNDLLIDELQS
                     TNEIENVFSTKQEIAHALNNQASEFLKFRGLVDQYKEIELNKKIKVDNVRDIRAIYDK
                     LVSNEINEQDKLDGELFRKNFVGVHDGSTNKYIHVGLQPETKIVEYIGEMLTFLKYFD
                     APQPFKIMASHYLFEYIHPFYDGNGRVGRFIIAKLLSDYYDNYTALTFSYVINRNKSK
                     YYKAFMTASNHLNCGDLTEFIDTMLELLIAGQERILDELIPKMDATEKLTLYLTSHYK
                     QIDYEFLYLLSMDKLFGNKRNRLT"
BASE COUNT         1797 a          665 c         1010 g         1656 t
ORIGIN      
        1 ttaagaaatt taaatataaa aattttgaag atgaaataga aattgacttc actgactcga
       61 atcatttggc tgcacacagt aatcaatcaa atcaaagtat gaacaataat gatttgacag
      121 cttcaaaagc gaatgataac tccgatgttt cgacaaatga ttatcatgac attaaatcac
      181 caatggtagg tacattcttt ttacaagata gtaaagaatt aactgaacca attgtgaatg
      241 tcggtgacaa agttaacaag ggagatatta taggatatgt tgaagcgatg aaagtattaa
      301 acgaggtaac aacagatgtt gctggagaaa ttactgaaat agtagctgat catggcacaa
      361 atgttgaata cgaccaagtt ttggtacgta ttaagtaaag ggggtaagta aatgcttcgt
      421 tgtttaattg cgaacagagg tgaaattgct gtaaggatta taagagcttg cagagaatat
      481 gggattgaaa ctgtagcagt ttatgcaaaa ggggacgagc aaagcttgca tgtacattta
      541 gcagatcaag ctatatgtat tggggaagct aatgctttag atagttattt aaatattgac
      601 cgcatcatat ctgctgcaca aatcactggt gctaatgcaa ttcacccagg atatggcttt
      661 ttatcagaat caacaaaatt tgctcaaacc gttgaggaac aaggcatagc gtttataggg
      721 cctactaaaa aaacgatgga aatgatgggg gataaaataa cagcaagaca aacagttcat
      781 catgcaggtg ttcctgtgat tccaggatca aatggtgctg tgaatcatgt gtctgaaatt
      841 gagaatcttg ccaaagacat cggttatcca gttgttatca aagctgccag tggcggtggt
      901 ggtaaaggta tacgtattgt aaagaaagct gaagatttag aaaaggcatt taaagaagct
      961 aaaagtgaag gaaaaaaata ctttgatgat gatcgtgttt atgttgaggc ttttatacct
     1021 gttgcaaaac atgttgaagt tcaagttatg ggagatgggc aggataatta tgtacattta
     1081 ggggaacgtg attgttctgt acaacgtaaa aatcaaaaat taatcgaaga atctccttgt
     1141 gcagcattaa ctgaagaacg acgacaacaa atatgtaacg atgctgttaa agttgcacgt
     1201 gcagcaaatt atagaagtgc aggtacgatt gaatttttag taactgatac cgcacattac
     1261 tttattgaaa tgaatgctcg aatacaagtt gaacatactg ttacagagat gcgagcagaa
     1321 cgtgatttgg ttgcagcaca actgtattta ttggaacata atcatttacc tttttcacaa
     1381 agcgatattc aatttaatgg tcatgtgata gaagcgagaa taaatgccga aaatcctgaa
     1441 aagaaatttc aaccaacacc aggtaaagtg acagcgttac atttacctca aggctttaat
     1501 gtgcgagtcg attctttact atatcacggt tatcaagttt caccatatta tgattcttta
     1561 gtagctaaag taattgtaaa atctcatgat agagcttcag caattgataa gttgaaagtc
     1621 accttagatg aaatggtgat agatggattt tctacaactg ccgatttttt atatgcagtt
     1681 ttaaattatc cactatatcg tgatggagat gcaaaagatg ttgatattaa atttttagaa
     1741 aaacatcaaa ttgttaaagg ggtggaatta taatgcgagt tgatttgaat tgtgatttag
     1801 gcgaagcatt tggaaattat tcctttggtg gtgatcatca aattattccg ttaattacaa
     1861 gtgcgaatgt tgcttgtggt tttcacgctg gtgatgaaaa tgtaatgaat gaaacggtaa
     1921 aacttgccaa agcacataat gttgcagtag gtgcacatcc tggtttacct gatttgaaag
     1981 gctttggcag acgaaatata gatatctcta acgacgagat ttataatttg atgatttatc
     2041 aattaggtgc attacaaggg ttttgtcgca ttcatcaact taaaattaat catgttaaac
     2101 cgcatggtgc attgtatcag atgggtgcaa aagacagaga aatagcaaac gttatagcac
     2161 aagctgttta tgactttgat ccatcactag tgttagtagg attagcaaat tcatatctaa
     2221 tttcagaagc aaagaatgtc ggattaatta cagcttctga agtgtttgct gatagacgat
     2281 acgaagatga tgggcagctc gttagtagaa aagaaagtga tgctgtgatt actgatactg
     2341 acgaagcact taagcaggtt ttaaagatgg tgaaggaaaa taaagttatt tcaaaaaaca
     2401 ataaggaagt aacgttacaa gcagatacaa tttgtgtgca tggtgatgga gaacatgcat
     2461 tattatttgt ttcgaaaatt agagaaattt taatgaaaga aggcattgat attcaatcct
     2521 tatagggggc taaaaaaatg gggagaaatt taaagcttaa aaaagaaagt gactttgaat
     2581 tcacaaaaaa tcataaaagg ttattattag gttctgtatt tttgatggca acttctgcaa
     2641 ttgggccagc atttttaacg caaacagcag tatttacatc acaatttttt gcaagtttcg
     2701 catttgccat attactgtct atcatcattg acattggtgc acaaattaat atatggcgca
     2761 tattagttgt aactggttta agaggtcaag aaatatcaaa taaagttgtt cctgggcttg
     2821 gtactgttat ctcaatattg attgcatttg gtggtctagc ttttaatatt ggtaatattg
     2881 ctggtgccgg tttaggttta aatgcaattt ttggattaga tgtaaaatgg ggcgcagcta
     2941 ttactgcaat ctttgcaata ttaatctttg taagtaaaag tggccaaaaa attatggacg
     3001 ttgtttcaat gattcttggt attgtgatga ttttagttgt ggcatatgtg atgtttgttt
     3061 ctaatccacc ttatggtgat gcttttgtgc atacatttgc gccagaacat ccaatgaaat
     3121 tagtcttgcc catcattacg ttagttggtg gaactgtagg tggttatatt acctttgcag
     3181 gtgcacatcg tatattagac tctggcatta aaggtaagca atatttacca tttgtaaatc
     3241 aatcagcaat tgctggtatt ttaactacag gtattatgag aacgttacta ttcctagcgg
     3301 tattaggagt tgttgtaaca ggtgtgacac taagttctga aaatccacca gcgtcagttt
     3361 ttgaacacgc aattggacca attggaaaga atatttttgg tattgtgtta tttgctgcag
     3421 ctatgtcatc agtaattggc tcagcataca caagcgcaac atttttaaaa acacttcata
     3481 aatcacttaa cgaaagaagt aatttaattg tgattgtgtt tatcgttatt tcaacaatga
     3541 ttttcttatt tattggaaaa ccaatcagcc ttttaattat agcaggcgcg ataaatggat
     3601 ggattttacc tattacgtta ggtgcaattt tgattgcaag taaaaagaaa tcaattgttg
     3661 gtgattataa acacccaaat tggatgttca tctttggtat cgttgcagta cttgttacaa
     3721 tattgacagg tatattctca ttcaaagaag tacttcagtt attttaaaag acatttcgac
     3781 taatagcatc actgtaaatg ttgttagtat taggcaagat atattttgat ttccggtgat
     3841 tcatttataa agcataatta gatatatttt aagggagagt ggtgcatgac aaatgtactg
     3901 ctctttttgt tgtgatgaaa aatctttttc tattgattga gaaaatagtt atttatatag
     3961 tttttaaaga aaaaaagtga taatatttag ctaatctaat gaaaatggat ttattggaca
     4021 aaaaatacat tttaaatgag tagaggaggc tgcaaatggg ttacagaact ttaaaaagta
     4081 tttttcatga acacaatgaa agtaaaatga aggaagagta tactaaaaga tttaattctt
     4141 tagcttcttt caatactaat attaatatca tacctatgga aaatggaaaa aaagttaatg
     4201 atttggaata tcctctattc tttatggtga ctaaaaatct atcaaaaaaa caagaattaa
     4261 tatcaattaa tagtagaaaa attgatagag cacttaattc tttaccatat gcagctagag
     4321 aacaatattt taatgattta ttaatcgatg aattacaaag tactaatgaa attgaaaatg
     4381 tatttagtac taaacaagag attgcacatg cgttaaataa ccaagcatca gaatttctta
     4441 agttcagagg cctcgtggat caatataaag agatagaact taataaaaaa attaaagttg
     4501 ataatgtaag agacattaga gcgatttatg ataaattagt ttcaaatgaa attaacgaac
     4561 aagataagtt agatggagag ctatttcgta aaaattttgt cggtgtgcat gatgggtcaa
     4621 cgaataaata tatacatgtt gggttacaac ctgaaaccaa aattgttgaa tatataggtg
     4681 aaatgctaac atttttaaaa tattttgatg cgcctcagcc gttcaaaatc atggctagtc
     4741 attatctgtt tgaatatata catccttttt atgatggcaa tggtagagtt ggaagattta
     4801 taattgccaa gctcttgagt gattattatg ataattatac tgctttgaca ttttcatatg
     4861 ttataaatag aaataaatcg aaatattaca aggcgtttat gactgcttca aatcatttga
     4921 attgtggtga tttgacggaa tttattgaca caatgcttga gctattgata gcaggacaag
     4981 aaagaatact tgatgagtta ataccaaaaa tggatgcgac tgaaaaatta acgctttatt
     5041 taactagtca ttataaacag atagattatg aatttttgta tctcctttca atggataaat
     5101 tgtttggaaa taaaagaaat agattaac
//
LOCUS       DAOBBH010000165         5121 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.165, whole genome shotgun sequence.
ACCESSION   DAOBBH010000165 DAOBBH010000000
VERSION     DAOBBH010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.165"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..281)
                     /locus_tag="P6L01_002158"
     CDS             complement(<1..281)
                     /locus_tag="P6L01_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDP3031208.1"
                     /translation="MTSKDITQISVIAAILTILAVLKIPSIIPGLDFQLSAPAALLIL
                     AFFGIKKYFLGGLLSSVLLLIFGVFNPINVIISIIFRVIAIAVVYLLKI"
     gene            complement(292..984)
                     /locus_tag="P6L01_002159"
     CDS             complement(292..984)
                     /locus_tag="P6L01_002159"
                     /EC_number="6.2.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxyhexanoate--CoA ligase"
                     /protein_id="HDP3031209.1"
                     /translation="MYSIKMRSSNQDVHISGAETICEFDKIEQTVQRFYNKGFFHENG
                     QPDFLNIKIQKIMEPIQQIKALQIIEDDKANLQHLTQECGVTEQALNQGMTYIKNETV
                     YTGAIILSAISGKRLDSFGQRGIRATHFSFEDINNKGDLNERVTDALAIASCINAHPY
                     VKGELCVSDDLTYTTGYFAAAKIGYHRLFDIKPVNTRYGGRIIFVDDCIDLNHYISFL
                     ESTPKQVVYETV"
     gene            complement(995..2110)
                     /locus_tag="P6L01_002160"
     CDS             complement(995..2110)
                     /locus_tag="P6L01_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HDP3031210.1"
                     /translation="MMFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVENKRCTNYTSND
                     YLGLGQIAFDKDDFERFMRKYSYHLSSSRLISGSSTAYEEIETMLAGWLGYSACTILN
                     SGYDANLALFNIFKNTNCVVFSDQENHASIIDGIKLSGLEKVIYKHLDIADLEKRLEK
                     YPNQNIPKIIISDSVFSTNGDVVDIGQLVSLKHKYNATLILDVSHSFGIENYSNYQGV
                     DILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPIYNLYFIKRNIEKLIN
                     ADDRRTKLNSLSKYFNQKLKALNVNYNSSNSPIKFIEFDDIEAAENIHQTLLKHHVFT
                     SYLRYPTVTKPMLRISLSYFHTEQDIDRLFEILHQED"
     gene            complement(2088..3095)
                     /gene="bioB"
                     /locus_tag="P6L01_002161"
     CDS             complement(2088..3095)
                     /gene="bioB"
                     /locus_tag="P6L01_002161"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDP3031211.1"
                     /translation="MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYF
                     GKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGT
                     YCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
                     HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFAL
                     KEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNL
                     RSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNDVSRA
                     N"
     gene            complement(3097..4455)
                     /gene="bioA"
                     /locus_tag="P6L01_002162"
     CDS             complement(3097..4455)
                     /gene="bioA"
                     /locus_tag="P6L01_002162"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDP3031212.1"
                     /translation="MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDT
                     NGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEI
                     TPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
                     SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEIMTAILTNIEQILIERNDEIA
                     GFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHED
                     VQPDIMCLGKAITGGYLPLAATLTSQKIYNTFLSDSHGVNTFFHGHTYTGNQIVCTVA
                     LENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKT
                     PLEIEKVERIVRKCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDR
                     KC"
     gene            complement(4433..5119)
                     /gene="bioD"
                     /locus_tag="P6L01_002163"
     CDS             complement(4433..5119)
                     /gene="bioD"
                     /locus_tag="P6L01_002163"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HDP3031213.1"
                     /translation="MRIFITSTNTDVGKTYVTKHLYHALKTRGHRVCVFKPFQTEERQ
                     DGTFPDLEVFKNECDLSYDITSLYTFKQPVSPHLAFKMTDQIFLNKQRVLDKVKVLDK
                     EFDFILIEGAGGIAVPIYEGTDDFYMTKDLINDCADCVISVLPSKLGAISDAVVHQDY
                     VNKNVSASNFLIMNRYTDSYIEKDNQITIGKLTNKTVYTFEEHATYENFSEAFLKQLI
                     GVKNELHTTT"
BASE COUNT         1596 a          992 c          667 g         1866 t
ORIGIN      
        1 tttattttca ataaataaac aactgcaata gctataactc taaatataat agaaataatc
       61 acattaattg gattaaatac gccaaatatc agtaataata cgcttgataa aagcccacct
      121 aaaaagtact ttttaattcc aaagaaagct aatatcaata atgctgccgg tgcagataat
      181 tgaaaatcta atcctggtat aatggacggt attttcaaaa ctgccaaaat ggttaaaatc
      241 gcagcaatga cactaatttg agtaatatct tttgatgtca tactaaaacc cctataccgt
      301 ttcataaaca acttgcttcg gtgtgctttc taaaaatgat atgtaatgat ttaaatcaat
      361 acaatcgtcc acaaatatta ttctgcctcc atatctcgta ttaactggtt taatatcaaa
      421 taatcgatgg taaccaattt tagcagcggc aaaataacct gtcgtatacg ttaagtcatc
      481 ggacacgcaa agttctcctt tgacatacgg atgcgcattg atacaactag caattgctaa
      541 ggcatcagtc actctttcat taagatcacc tttattattt atatcttcaa acgaaaaatg
      601 tgttgcccta atcccccttt gtccaaatga atctaaacgt ttaccagata tagcagatag
      661 aataatagct cctgtataaa ccgtttcatt tttaatatat gtcatccctt gatttagcgc
      721 ttgttcagtg acaccacatt cttgtgttaa atgttggaga tttgctttat catcctcaat
      781 aatttgtaat gcttttattt gttgaatcgg ttccataatt ttttgtattt ttatattgag
      841 aaaatccggt tgcccatttt catgaaaaaa acctttatta taaaaccttt gtaccgtctg
      901 ttctattttg tcaaactcac aaatcgtttc agcaccacta atatgaacat cttgattgct
      961 agaacgcatt tttatactat acatgacgat cacctcaatc ttcttgatgc aaaatttcaa
     1021 acaacctatc tatatcttgt tcagtatgaa aatacgacaa tgatattctt aacattggct
     1081 tagtcacagt tggatacctt aaataacttg taaacacatg atgctttaat aatgtttgat
     1141 gaatgttctc agccgcttct atgtcatcaa actcaataaa cttaatcggc gagtttgaac
     1201 tattataatt aacattgagt gcttttaact tttggttaaa atatttactc aaactattta
     1261 atttagtgcg tctatcatca gcatttatta acttttcaat gtttcttttt ataaaataca
     1321 aattataaat tggcaaacta cttgagtaga tgagtggtct accgtgatta attaacatat
     1381 ccttcacatc atttgaactt aaaatcacac ccccgtatgc accacatgct ttagataaac
     1441 tagaagtgag tatatctaca ccttgataat tcgagtaatt ctctattcca aaactatgtg
     1501 aaacatcgag tatcagtgtt gcgttatatt tatgctttaa tgagactaat tgaccaatat
     1561 ccacaacgtc gccattcgtt gaaaatacac tatcagatat gattattttt ggtatatttt
     1621 gattagggta tttctctaac cttttttcta aatcagcaat atctaaatgc ttatatatca
     1681 ctttttctaa accacttaac ttaataccgt caataatact cgcatgattt tcttgatctg
     1741 aaaacacgac acaatttgta tttttgaaaa tattaaacaa cgccaaatta gcatcataac
     1801 cactatttaa gatagtacat gcactatatc cgagccaacc tgctaacatt gtttcaattt
     1861 cttcataagc tgtcgaactt ccactaatta atcttgaact tgataagtga taactatact
     1921 tccgcataaa tctttcgaaa tcatccttat caaacgctat ttgacctaat cctaaataat
     1981 cattagatgt atagttcgta catctcttat tttctacttc aatatactgt ctatctatat
     2041 accctaccga tttaagcgac cgatataacc ctttctgttg taataaatca atttgctctt
     2101 gaaacatcat tcttgttttc cttattttca caagtgtcat aatcaatttc aaagcccaaa
     2161 tcattaatca tatcgtagtc taattggttc ggttgcccac cagtaattag ataatcaccg
     2221 acaaatattg aattcgccgc ttttaatgct aatggctgta acgaacgtaa gttgacctct
     2281 cttcctccag caatacgaat ttctttcgta ggattgatta atcggaataa tgctacgatt
     2341 cttaaacatt tcattggtgt taaatcatcc atgcttccaa actttgtgcc tttgattgga
     2401 tgcaaaaaat taatcggaat actgtcggca tccatttctt ttaaagcaaa tgccatatca
     2461 acaatatctt gattagattc tcccatacca caaatcacgc cagaacatgg tgatatatta
     2521 ttcgctttca ttagttctat cgtatctgtt ctatctttat aactatgcgt tgtcacgacg
     2581 ttatcatggt aattttcact tgtattaata ttgtggttat atctgtctac accagctgac
     2641 ttaagtttct tagcttgttc gtcattcgtt aatcctaaac atgcacagat ttttagttgc
     2701 gggtgttgag atttaatcgt tcttacagta ttactaatat gatcaacttc tttatcgctc
     2761 ggtcctctac cactcataac aatacaatat gttccaatat gattatcatg tgccaccttt
     2821 gctccatcga taatttgttc ctctggaatt aaagcatatc gctgtttttg tttaatatct
     2881 cgtgattgtc cacagtaccc acaattctca ggacatatac cacttttagc atttaaaatc
     2941 atgtttaatt ttactttttt accaaaataa tgttttctta aaatgtacgc ctcatttaat
     3001 aaatctaagg tatcaatatt agtatcctca taaattttca atacagtctc ttttgttaat
     3061 tgttcccctt gtaatatgcg tttagccaaa ttcatattaa cacttcctat ctaaaatgtt
     3121 atgtactgcc tttttaaaaa tacgtaccat cgttttcact tctttatttg acatacttaa
     3181 cactggcaca aacgtaatga cattttctaa atttctaatc attagcccgt tttctttaca
     3241 tttacgtaca atacgttcaa ctttttcaat ttctaacggc gttttactat ctttatctgt
     3301 aactaattca actccaaaca ttaagcctcg tcctcttaca tcaccaacat ttcgatgacg
     3361 cttcagcgca tgtaactgtt tctcaagtgt tgatgatgtc gtttcaatat gtgacaataa
     3421 cttacgtttt tcataaagtc ttatattttc taatgcaacc gtacaaacga tttgatttcc
     3481 ggtgtatgta tgaccatgga aaaaggtatt aacaccatgc gaatcactta aaaatgtatt
     3541 gtatattttt tgagatgtca atgtagctgc aagtggtaag tagccaccag taatcgcctt
     3601 acctaaacac ataatatccg gttgaacatc ttcatgattg catgcaaaca tctttccagt
     3661 tctcccaaaa ccaactgcta cctcatcaca aattaataag acatcgtatt ttttgcacaa
     3721 tttctcgact tctttcaaaa agcctttagg atgaacaaac aagcctgtcg caccttgaat
     3781 caacggttcc aatataaacc ctgcgatttc atcatttctt tcaattagaa tttgctctat
     3841 attcgttaaa atagctgtca taatttcatt ttcagtatcg taattactgc gatagaaaga
     3901 tgggctttct acttgaatat tctcaaatat taagtcttta aatattttat gaaaggtctt
     3961 gataccacca acacttaccg caccaatcgt atccccatga taaccgtgat ttagcgttat
     4021 aaacttgttt ttcttggcat atttttctct atcaatattt ttccaatact gatatgccat
     4081 ctttattgcg atttcaacag acgcactgcc tgtatcagaa taaaatactt ttcttagatt
     4141 acttggcgtg atttcgatta atttttccgc aagttctatt gacggaatat ttgatgatcc
     4201 tagcagcgta gaatgggcaa ttttattgag ttgcttttta attaccttat tcaagtattt
     4261 gttattatga ccatgcacat tgacccacaa cgatgcataa ccatctaaat atttattgcc
     4321 attcgtatcg taaaggtaac taccctttcc tttttcaatg atgattgctt cttctttgct
     4381 atatacaccc atttgtgtaa atggatgcca aacatattct gagtcttttt gtttaagttg
     4441 ttgtgtgtaa ttcattttta actcctatta attgttttaa aaatgcttct gagaaatttt
     4501 catacgtggc atgttcttca aatgtataga ctgttttatt tgttaatttt ccaatcgtaa
     4561 tttgattgtc tttttcaata tagctgtctg tatagcgatt cattattaaa aaattactcg
     4621 ccgatacatt cttattaaca taatcttggt gaacaacggc atcgctaata gcacctaatt
     4681 ttgatggcaa cacactgatg acacaatctg cacaatcatt gattagatct ttagtcatgt
     4741 agaaatcatc tgtaccttca tatattggta cggcaattcc cccagcaccc tcaattaaga
     4801 taaaatcaaa ttccttatct aaaactttta ccttatctaa tacacgctgc ttatttagaa
     4861 aaatttgatc tgtcatttta aatgcaaggt gtggtgatac aggttgctta aaagtataaa
     4921 gtgacgttat gtcatagctt aaatcacatt catttttaaa tacttctaaa tctggaaatg
     4981 tcccgtcttg gcgttcctca gtttgaaatg gtttaaaaac acaaacacga tgaccacgtg
     5041 ttttcaaagc atggtataaa tgctttgtaa cataggtttt gcctacatca gtattcgtac
     5101 ttgtaataaa aatcctcata a
//
LOCUS       DAOBBH010000166         5113 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.166, whole genome shotgun sequence.
ACCESSION   DAOBBH010000166 DAOBBH010000000
VERSION     DAOBBH010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.166"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(53..1123)
                     /gene="dinB"
                     /locus_tag="P6L01_002164"
     CDS             complement(53..1123)
                     /gene="dinB"
                     /locus_tag="P6L01_002164"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDP3031214.1"
                     /translation="MTERRIIHIDMDYFFAQVEMRDNPKLKGKPVIVGGKASSRGVVS
                     TASYEARKYGVHSAMPMSQAHKLCPNGYFVTSNFGAYRETSAQIMSIFRSYTDKVEPM
                     SLDEAYLDITELVRPDLPASKIAQYIRKDILEQTHLTASAGVSYNKFLAKLASGMNKP
                     DGLTVIDYQNVHDILMTLDIGDFPGVGKASKKVMHDNGIFNGRDLYEKTEFELIRLFG
                     KRGRGLYNKARGIDHSEVKSSRVRKSVGTERTFATDVNDDEEILRKVWELSGKTAERL
                     NKLQKSAKTVTVKIKTYQFETLSKQMSLRDSVSSEEDIYNIAYLLYNDLKDPDVPIRL
                     IGVTVGNLEQSTYKNMTIYDFI"
     gene            complement(1370..1900)
                     /locus_tag="P6L01_002165"
     CDS             complement(1370..1900)
                     /locus_tag="P6L01_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HDP3031215.1"
                     /translation="MHKIDLTTNNFQMRRFIILQLVIALFVILFTYKWALGVTAVVDQ
                     NIIINLVYGFAGFIILLILHELIHRALFLLFKKDSKPMFNIKKDRILFQTADACFNKW
                     QFSIIMLSPLILLSTGLLILIKVFGYSSLIFMFSMHTAYCFIDILLVALTISSSFKYV
                     QQDEDSIYLYHQKPTQ"
     gene            complement(2070..3431)
                     /gene="rlmD"
                     /locus_tag="P6L01_002166"
     CDS             complement(2070..3431)
                     /gene="rlmD"
                     /locus_tag="P6L01_002166"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDP3031216.1"
                     /translation="MQAIAKNDIKTGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIE
                     YKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYEAQLEMKKEQVI
                     NLFQRKAHFDNSKINDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDME
                     SCLIQDSQHQEVMNEVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMIIFVTN
                     GKKWPQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDST
                     FKISDQSFYQINSEQTEKLYNKAIEYAQLTGNEVVLDTYCGIGTIGLYMAPHAKHVYG
                     VEVVPSSIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKPDVVMVDPPRKGCDE
                     TFIQTLLTLEPKRIVYISCNPATQQRDALLLAEKYQLEEVTPVDMFPQTTHVETVALF
                     NLK"
     gene            complement(3512..4459)
                     /locus_tag="P6L01_002167"
     CDS             complement(3512..4459)
                     /locus_tag="P6L01_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDP3031217.1"
                     /translation="MRKRARIIYNPTSGKELFKRELPDALIKLEKAGYETSAYATEKI
                     GDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRAL
                     HIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
                     AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGY
                     FTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGG
                     KLPANFLNLERHIDVFAPNDIVNEDLINNDHVDDNLIEE"
BASE COUNT         1599 a          956 c          703 g         1855 t
ORIGIN      
        1 tcccttcaaa agtaagcatt gtaaaaatgt aaactttgca gggagcttta ttttatataa
       61 agtcatatat cgtcatattt ttataagttg attgttctaa attacctaca gtgacaccaa
      121 taagtcgaat tggtacatct gggtctttta aatcgttata aagtaaatat gcaatattat
      181 aaatatcttc ttcagaacta accgaatctc ttaaactcat ctgtttagat agcgtttcaa
      241 attgataagt tttaatttta accgttacag ttttagctga cttctgtaat ttatttagac
      301 gttcagctgt tttacctgac aattcccata cttttcttaa aatctcttca tcatcattca
      361 cgtctgttgc aaatgtgcgt tcagtcccta ctgattttct tactcttgat gatttcactt
      421 cactatggtc aataccgcgt gccttgttat ataaaccccg acctcttttt ccaaacaaac
      481 gtattaattc aaattccgtt ttctcatata aatctctacc gttaaaaata ccattatcat
      541 gcattacttt tttggaagct ttacctacgc ctggaaaatc tccaatatcc aatgtcatca
      601 aaatatcatg gacattttga taatcaatca cagtcaaacc atcaggttta ttcataccac
      661 tcgctaattt agctaaaaat ttgttataag aaacacctgc agatgctgtt aaatgtgtct
      721 gctctagaat atcttttcta atatactgag caattttcga agcaggaagg tctggtctca
      781 ctaattctgt aatatctaaa tacgcttcat ccaatgacat cggttctacc ttatctgtat
      841 aacttcggaa aatagacata atctgcgcag atgtttctcg gtaagcacca aaattacttg
      901 tgacaaagta tccatttgga cataatttat gcgcttgtga cataggcatt gctgaatgga
      961 cgccgtattt tcgtgcttca taggatgccg tagagacaac acccctactg cttgctttac
     1021 caccaacaat gactggtttc cctttcaatt tggggttatc tctcatttcg acttgtgcaa
     1081 aaaaatagtc catatctata tgaataattc gtctctcagt caagtgctca cctccctact
     1141 aatttttact tttataacgc acaaaaatat ctcaacataa ttatacgctg tgtacgattt
     1201 ttttacataa atcttgcact tagcgataac tatattgaga taactacaag ttgttataaa
     1261 atcaattgct atttaagcat gatgatgaag acgattgagt aagaaaacat aggtaatctg
     1321 aaataattca agcaaattca ttttgttggt attatcatat taaaatttat tattgagtcg
     1381 gcttttgatg atacaaataa atactatctt catcttgttg aacatattta aaactactag
     1441 atattgttaa tgcaacaagc agaatatcta taaagcaata agcggtatgc atactaaaca
     1501 taaaaataag tgatgaataa ccaaatacct taattaaaat aagcaagcca gtacttaata
     1561 agattagtgg tgacagcata ataattgaga attgccattt gttgaagcaa gcatctgctg
     1621 tttggaataa gattctgtct ttttttatat taaacatagg tttgctatct tttttaaata
     1681 aaagaaataa tgctctatgg ataagttcat gtaaaatcaa taaaataatg aatccagcaa
     1741 acccatatac aagattgatg atgatatttt gatcgacaac cgctgtgaca cctaacgccc
     1801 acttatacgt aaataaaatc acgaataacg caataacaag ttgcaagata ataaaccttc
     1861 gcatttgaaa attatttgtc gttaaatcaa ttttatgcat taccaaccct cccgatcatg
     1921 acattcttat tcttctttaa atatagtata caatgtcaca tttaatttaa aaagttcata
     1981 tcaagaaagt aaattggctg taataaaatt ttaatatacg acttctttct tcacttatta
     2041 aggcgaaatt ttatctcaaa tcatgtgcgc tatttcaaat tgaataatgc cactgtctca
     2101 acatgtgttg tttgtggaaa catatctacc ggtgttacct cttcaagttg atatttttca
     2161 gctaataata atgcatcacg ttgctgtgtt gcgggattac atgaaatata gacaatacgc
     2221 ttaggttcta atgtaagcaa agtctgaata aacgtttcgt cacagccctt tcttggcgga
     2281 tcaaccatta caacatctgg tttaatccct tgtgctttcc attgtaaaat aacttcttca
     2341 gctttcccac agacaaaagt tgtattattg cattggttta tagtcgcatt ttgttgtgcg
     2401 tcttcaattg aagaaggtac tacttcaaca ccgtatacat gttttgcatg tggtgccata
     2461 tatagcccta ttgttccaat accacaatag gtatctaata caacttcatt acctgtcaat
     2521 tgcgcatact caattgcttt attatataat ttctctgttt gttcagaatt aatttggtag
     2581 aatgactgat cacttatttt aaatgtacta tctgttaatt gatcaataat tgtatcttta
     2641 ccatatagcg ttatagattg acgtcccata ataacattag agtggctatc attaatgttt
     2701 tgtttaatgc ttgtcacatt aggaaatgca tctaatatct tctcaacaac agcatttttt
     2761 tgtggccact ttttaccatt agttacaaaa ataatcatca tttcgtctgt atgatatcct
     2821 gttcttacaa ccaaatgtct cattaaacct tttttcaatt gttcttgata aatacttaca
     2881 tttaaatctt ttaaaataga tttaacttca ttcatcactt cttgatgttg tgaatcttgt
     2941 attaaacaac tttccatgtc aataatgtca tggcttcttt gacgataaaa gcccataata
     3001 acttcattct gttcattctt accaactgga atctgggact tgtttcgata tctccaagga
     3061 tctgtcatgc caactgtatc gttaatctta gaattatcaa aatgcgcttt tcgctgaaac
     3121 aaattaatca cttgttcctt tttcatttca agttgtgctt cgtatgataa gtgttgaagt
     3181 tggcacccac cacaacgttc ataatatata catggcggtg caacacgatt ctcacttctc
     3241 gtatttatat taagcaactt acctatcgca aagttctttt ttactttgat aattttatat
     3301 tcaatttgtt cattaattaa agcttgtggt atgaaaatag gaaagcgatc tatttttaca
     3361 acaccatggc cttcatgcgt taaatcaaca actgttcccg tttttatgtc atttttagct
     3421 attgcttgca aaattttacc tccaaaatga acaggttagg aacaaaatta tgcgcttcct
     3481 aacctgccat tatatatttc actatttctg tttattcttc gattaaattg tcatcaacat
     3541 gatcattatt tattaaatct tcatttacaa tatcattagg tgcaaagaca tctatatgac
     3601 gttctagatt taagaaattc gctggtaatt taccaccata ttctccatct acatttagtt
     3661 gtaagtctgt gaatgatgaa atattaattg cctttgcttt ttcataaata actttaggat
     3721 gcttagtatg ttctcctctt gaagctaaag tcataatatg accaagttct gcaaggtttg
     3781 atttttcaac tataattaac gtaaaatagc cgtcatctaa cttagcgtcc ggcactaatt
     3841 tttcaaatcc tgccattgaa tttgttaaac ctaaaaagaa taataatgct tctccttgga
     3901 aaacattacc atcatattca attcttaaat ctacagcttt catttgaggt aacatttcga
     3961 aacctttgat gtaataagca aatggaccaa caatagattt caatttactc ggtgtttcat
     4021 aagagacttg cgtcaattgt ccgcctgcag ctaaattaat aaagtatcga ttattcattt
     4081 taccaatatc tactttagta gaatgacctt caatgatgac atcaagtgcc cccatgatgt
     4141 cattaggtat atgcaatgca cgtccaaagt cattaacagt acccatagga atgacaccta
     4201 gcttaggacg attaggcttt tctgcgatac cattaactac ttcatttaat gttccatcac
     4261 cacctgcagc gattaatacg tcataatttt catgcatagc tctttctgct tcaagtgtgg
     4321 catcacctat tttctcggtt gcatatgcac tcgtttcata tcccgctttt tctaatttta
     4381 ttaaggcatc aggtaattct cttttaaata gctctttacc tgatgtcggg ttataaatga
     4441 ttctagcacg tttcctcata tcttatccct ctacttaaaa ttcatatatt ttaacttcat
     4501 ctttgtttcg tctaataggg agtgggacag aaataatatt taacaaaatt tatttcgttc
     4561 taccccaact tgcattgtct gtagaatttc ctttcgaaat tctctttgtt tggggcccca
     4621 ccccaacttg cacattattg taagctgaca gaaagtcagc ttctatgttg gggccccgcc
     4681 aacttgcaca ttattgtaag ctgacagaaa gtcagcttct atgttggggc cccactagaa
     4741 ttgaaaaaag cttgttacaa gcgtattttc tttcagtcaa ctactgccaa tataacattg
     4801 tagtgcctag gacattgaat ttatgaccca ggctcagtct tatttcatca ttcttaatat
     4861 cgttaaagac caacttgtat cttaaacaaa tactatctca atatgtacaa agcttgttat
     4921 ttattcagca ttttttgccg ttcttcatta tatagacttc gtcagttatg ctattttacc
     4981 tttaaaatga tgttgtaaat ataatgttgt ctttatacaa aaatgtttta taccttttta
     5041 ttatacagtt tgccttaata taattaaagc taacgcatta ataaaattaa tatttttacc
     5101 attaacatgt aca
//
LOCUS       DAOBBH010000167         5096 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.167, whole genome shotgun sequence.
ACCESSION   DAOBBH010000167 DAOBBH010000000
VERSION     DAOBBH010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.167"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(72..248)
                     /locus_tag="P6L01_002168"
     CDS             complement(72..248)
                     /locus_tag="P6L01_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDP3031218.1"
                     /translation="MSKTVVRKNESLEDALRRFKRSVSKSGTIQEVRKREFYEKPSVK
                     RKKKSEAARKRKFK"
     gene            complement(541..1887)
                     /gene="mtaB"
                     /locus_tag="P6L01_002169"
     CDS             complement(541..1887)
                     /gene="mtaB"
                     /locus_tag="P6L01_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HDP3031219.1"
                     /translation="MSTVAFHTLGCKVNHYETEAIWQLFKEANYERVDFEANADVFVI
                     NTCTVTNTGDKKSRQIIRRAIRQNPDAVICVTGCYAQTSSAEIMEIPGVDVVVGTQDR
                     HKLLGYIDEFRKERQPINGVGNIMKNRKYEELDVPYFTDRTRASLKIQEGCNNFCTFC
                     IIPWARGLMRSRDPEKVVEQATQLVNSGYKEIVLTGIHTGGYGQDLKDYNLAQLLRDL
                     ETINGLERIRISSIEASQLTDEVIDVLERSTKVVRHLHIPLQSGSDTVLKRMRRKYTM
                     DRFSERLTKLHKALPDLAVTSDVIVGFPGETEAEFQETYDFIVKHKFSELHVFPYSPR
                     IGTPAARMDDQIDEEIKNERVHKLITLSNQLGKLYASKFDQDVLEVIPEEQGDTEGTL
                     VGYADNYMKVQFEGDESLIGQIVKVKITQANYPLNEGQAIKVVDFATNKSDREVLV"
     gene            complement(1894..2646)
                     /locus_tag="P6L01_002170"
     CDS             complement(1894..2646)
                     /locus_tag="P6L01_002170"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDP3031220.1"
                     /translation="MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFN
                     DQTVYLCEIADITSDKIAVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELG
                     ASSFIAVSMERSIVKLNEAKVVKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICD
                     TIDNYDYILIAYEEEAKDGELSNFKQTLQQFKAQDKVLMIFGPEGGLSENEISLFSNT
                     STVVGLGPRILRAETAPLYALSAISYEKELMG"
     gene            complement(2648..3586)
                     /gene="prmA"
                     /locus_tag="P6L01_002171"
     CDS             complement(2648..3586)
                     /gene="prmA"
                     /locus_tag="P6L01_002171"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDP3031221.1"
                     /translation="MNWTELSIIINHEAVELATNILENHGSNGVVIEDSDDLINQPED
                     KYGEIYALKKEDYPDKGVRLKAYFNEMTYDDKLRQQIKDELLNLDELDQHNIQFSEQI
                     IAETDWENEWKNYFHPFRASKKFTIVPSWETYAKEADEELCIELDPGMAFGTGDHPTT
                     SMCLKAIETYVLPQHSVIDVGTGSGILSIASHLIGVKRIKALDIDEMAVSVAKENFRR
                     NHCETLIEAVPGNLLKDETEKFDIVIANILAHIIDEMIEDAYNTLNEGGYFITSGIIK
                     EKYEGIQSHMERVGFKIISEQHDNGWVCLVGQKVSE"
     gene            complement(3590..4729)
                     /gene="dnaJ"
                     /locus_tag="P6L01_002172"
     CDS             complement(3590..4729)
                     /gene="dnaJ"
                     /locus_tag="P6L01_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDP3031222.1"
                     /translation="MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADE
                     KFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFSGGGFEDIFS
                     SFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPG
                     TSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKT
                     VKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKL
                     NVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDI
                     KVVTPTKLTDRQKELMKEFAQLNGEEINEQPSNFKDRAKRFFKGE"
     gene            complement(4865..>5096)
                     /gene="dnaK"
                     /locus_tag="P6L01_002173"
     CDS             complement(4865..>5096)
                     /gene="dnaK"
                     /locus_tag="P6L01_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500193.1"
                     /note="heat shock protein 70; assists in folding of
                     nascent polypeptide chains; refolding of misfolded
                     proteins; utilizes ATPase activity to help fold;
                     co-chaperones are DnaJ and GrpE; multiple copies in some
                     bacteria; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDP3031223.1"
                     /translation="AEEKKDALKTALEGQDIEDIKSKKEELEKVIQELSAKVYEQAAQ
                     QQQQAQGANAGQNNDSTVEDAEFKEVKDDDKK"
BASE COUNT         1479 a         1027 c          679 g         1911 t
ORIGIN      
        1 tcagacacta acttgtgatg tttattttat ataattaata ttcgtgttga gggagtcaac
       61 agaggtatta attatttgaa tttacgttta cgtgcagctt ctgatttctt tttacgtttt
      121 acgcttggtt tttcgtaaaa ttcacgttta cgtacttctt ggattgttcc acttttagaa
      181 actgaacgtt taaatctacg taacgcatct tcaagtgatt catttttacg tactactgtt
      241 ttagacatct gtatttccct ccctccaaat atcaacggaa ctttatagtc aattgcacag
      301 taaaactatg catttataag tataatatat ctgtaattta tggtcaatta gtaaattgtt
      361 ttttatttga aacatatttc acatcaaaat cacaaagact tttagatttt gttctaaaaa
      421 tctcttaata atttatttaa tgagaagagt tgcttatata gtaaattgtg aagccgttaa
      481 aacaacgtta caaaacctat atctttagta cggaacctta tggtatgaat caaggaatac
      541 ttaaactaaa acttctctat cagatttatt tgttgcgaaa tcaacaactt taattgcttg
      601 cccttcattt aatggataat ttgcttgcgt aattttaact tttacaattt gacctatgag
      661 tgattcgtca ccttcaaatt gtactttcat ataattatct gcatatccaa ctaatgtacc
      721 ttctgtgtca ccctgttcct caggaattac ttcaagcaca tcttgatcaa atttagacgc
      781 atataacttt ccgagttgat tgcttagcgt aattaactta tgcacgcgtt catttttaat
      841 ttcttcatca atttggtcat ccattcttgc agctggcgtg ccaattctag gagaataagg
      901 gaaaacatgc agttcagaga acttatgctt tacgataaaa tcatatgttt cttggaactc
      961 tgcttcagtt tcacctggga aaccaacaat tacatcactc gtaactgcca agtctggtaa
     1021 agctttatgc aattttgtta atcgttctga aaatctatcc attgtatact tacgtctcat
     1081 acgttttaat actgtatctg aaccagattg taatggaata tgcaaatgac gcacaacttt
     1141 tgttgaacgt tctaaaacgt caattacttc atctgtaagt tgacttgctt caattgaaga
     1201 aattcgaatt cgttctaatc cattaatcgt ttcaagatca cgtaataatt gggccaagtt
     1261 ataatctttt aaatcttgac catatccacc tgtatgaatt cccgtcaata caatttcctt
     1321 atatcctgaa ttcactagtt gcgtcgcttg ttcaactact ttttccggat ctcttgaacg
     1381 cattaagcca cgagcccatg gaataataca gaatgtgcag aagttgttac aaccttcttg
     1441 aatttttaat gacgcacgtg ttctatctgt aaaatatgga acatctaatt cttcatattt
     1501 acgatttttc atgatatttc caacaccatt aattggttgg cgttctttac ggaattcatc
     1561 aatgtaacct aatagtttat gtctatcttg tgtaccaact actacatcga caccaggaat
     1621 ttccataatt tcagctgatg aagtttgcgc ataacaacct gttacacaga ttacagcatc
     1681 aggattttgt cttattgcac gtctaattat ttgacgactt tttttatcac ctgtattcgt
     1741 tactgtacaa gtattaataa caaatacatc agcattcgct tcaaagtcaa cgcgctcata
     1801 gtttgcttct ttaaataatt gccagattgc ttcagtttca taatggttta ctttacaacc
     1861 taatgtgtga aacgcaactg ttgacataaa tattcacccc attaattctt tttcataact
     1921 tattgcactt aacgcataca atggcgcagt ttctgcccgt aaaattctcg gtccaagacc
     1981 aacaactgta ctagtattac taaataatga aatttcattt tctgacaaac caccctcagg
     2041 accaaaaatc atcaacactt tatcctgagc cttgaattgt tgtaaagttt gcttgaaatt
     2101 gcttaactca ccatcttttg cttcctcttc atatgcaata agaatatagt cataattatc
     2161 aatagtatca caaattaatt ttaaattcga ctcgaattga atagatggaa tcactaaacg
     2221 atagctttgt tcagcagctt ctttaattat tttttgccaa cgctctatct ttttgacaac
     2281 tttcgcctcg tttaatttaa caattgagcg ttccatgctc acagctataa atgatgaagc
     2341 acccaattca gtagcttttt gtagcaacca ctcatatttg tcagctttaa ttagtccact
     2401 gcaaatcgta acatcaactg gcaattctgt attaatattt tgtttttctt ttaaatcaac
     2461 tgcaatttta tcacttgtta tgtcagcaat ttcacataaa taaactgttt gatcattaaa
     2521 agttaaaata attttactac caacatcata tctcattaca tttgttatat gatgaatatc
     2581 ttcttttttt gtaataaaaa aacgctgact tacatcagcg ttttggtcta tgaaataacg
     2641 ttgcacatta ttcactcact ttctggccaa caagacaaac ccaaccattg tcatgttgtt
     2701 ctgaaataat tttaaaacct acacgctcca tatgtgactg tataccttca tacttctctt
     2761 ttataatacc agaagtaata aaataaccgc cttcatttag agtattataa gcatcttcaa
     2821 tcatttcatc aataatatgc gctaaaatat ttgctattac aatatcaaat ttttctgttt
     2881 cgtctttcaa taagttacct ggaacagctt caattaacgt ttcacaatga tttcttctga
     2941 agttttcttt agctacactc actgccattt catcaatatc caacgcttta atacgtttta
     3001 caccgattag atgacttgca atacttaata tacctgagcc agtaccaaca tcaattactg
     3061 aatgctgtgg caatacatat gtttctattg ccttcaaaca catacttgta gtcggatgat
     3121 cgcctgttcc aaaagccata cctgggtcga gctcaatgca aagctcttca tccgcttctt
     3181 tagcatatgt ttcccaacta ggaactattg tgaacttctt cgacgctcgg aatggatgga
     3241 aatagttttt ccattcattt tcccaatccg tctctgcaat aatttgctca ctgaattgaa
     3301 tgttatgttg atcaagttca tctaaattta ataactcatc tttaatttgc tgtcgcaact
     3361 tatcatcata agtcatttca ttaaaatagg ctttcaatct tactccctta tctggataat
     3421 cctctttttt caaagcgtaa atttcaccgt atttatcttc tggttggtta attaaatcat
     3481 ctgaatcttc tatcacgaca ccatttgatc catgattttc aagtatattg gtagccaatt
     3541 ctactgcttc atgattaata ataattgaaa gctctgtcca gttcatactt tattctccct
     3601 taaagaatct ttttgctcta tctttaaaat tcgaaggttg ttcattaatt tcttcaccat
     3661 ttaattgggc aaattctttc attagttctt tttgtctatc tgttaattta gtaggcgtta
     3721 ctactttaat atcaacatat aaatctccgt atccatagcc atgaacattt tttataccct
     3781 tttcttttaa gcggaattgc ttacctgttt gtgtaccagc agggattgtt aacataactt
     3841 cattatttaa tgttggtatt tttatttcat cgcctaaagc tgcttgtggg aagctaacat
     3901 ttaatttgta ataaatatca tcaccatcac gtttaaatgt ttcagatggt ttaactctaa
     3961 atactacgta taaatcacca gcaggtcctc cattcacgcc tggagagcct tcaccagcta
     4021 atctaatttg ttgttcattg tcgacacctt caggtacttt cacttctaat ttaactgttt
     4081 tattttcagt accttttccg tgacatgttg gacaagcttc ttcaaattct tgaccacttc
     4141 cattacattt aggacaaact tgttcagtac gaactctacc taaaattgtg ttttgttcta
     4201 cagctacatg accagcgcca ttacagtaac tacaagtctt tttacttgtt ccaggctttg
     4261 caccatcacc atgacatgtt tcgcatgtta catctttacg gattgaaatt tcttttgttg
     4321 taccaaatac cgcttcttca aatgttaatg tcattgtata ctgaagatca tcaccttttt
     4381 gcggtgcatt tggatctctt tgtctgccgc caccgaagaa agagctaaag atatcttcaa
     4441 aaccgccgcc actgaagcca ctaaaaccgc caaagtcaga gccattgaat ccttgtccac
     4501 caaaaccttg tggaccatca tgtccaaatt gatcatagct tgcgcgttta ttatcatcac
     4561 ttaaaacttc ataggcttca gaaatttctt taaacttttc atctgcacct tcttctttgt
     4621 taatatctgg atgatatttt ttcgaaagct ttcgatacgc ttttttgatt tcatcttttg
     4681 aagcatcttt actaatgcct aaaacttcat aatagtctct tttggccaca gctatctctc
     4741 cttttcttaa ttaactcata tagtttaacg taatatgtca tactatccaa ataaaaagcc
     4801 aaagccaatg ttctattgac tttgactttt cagatcatga caacattcta attgtattgt
     4861 ttaattattt tttgtcgtcg tcttttactt ctttaaattc agcatcttct acagtactat
     4921 cgttgttttg accagcattt gcaccttgtg cttgttgttg ctgttgagcc gcttgctcat
     4981 atacttttgc tgataattct tgaatcactt tttcaagttc ttctttttta gatttaatat
     5041 cttctatatc ttgaccttct aaagcagttt taagagcgtc ttttttctct tcagca
//
LOCUS       DAOBBH010000168         5012 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.168, whole genome shotgun sequence.
ACCESSION   DAOBBH010000168 DAOBBH010000000
VERSION     DAOBBH010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.168"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            154..1026
                     /gene="dprA"
                     /locus_tag="P6L01_002174"
     CDS             154..1026
                     /gene="dprA"
                     /locus_tag="P6L01_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDP3031224.1"
                     /translation="MIKLFLLKLYWAHFSTKQIHQFLMAYPNVIKEEGRKKDSYLCEW
                     VNREENVLLLRKYYAFIKLDHNDIIKELQKLKVSYITYMDSEYPVLLKEIYQFPLLLF
                     YKGNIKLINNMHHLAVVGARDSTSYTQQSLEFLLSNDKSKYLTIVSGLAQGADAMAHQ
                     IALKYNLPTIAVLAFGHQTHYPKSTLALRNKIEEKGLVISEYPPHTPIAKYRFPERNR
                     IISGLSKGVLITEAKEQSGSHITIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGAK
                     VVLNANDIFEDYYI"
     gene            1200..3275
                     /gene="topA"
                     /locus_tag="P6L01_002175"
     CDS             1200..3275
                     /gene="topA"
                     /locus_tag="P6L01_002175"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDP3031225.1"
                     /translation="MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQM
                     GVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILEL
                     EDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
                     KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPF
                     KLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKT
                     MMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKAEAKQYITDKYGESYTSKRKA
                     SGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVS
                     LDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPA
                     QHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEI
                     VHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEME
                     KIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCND
                     GDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSN
                     CDYKEAAQK"
     gene            3431..4738
                     /gene="trmFO"
                     /locus_tag="P6L01_002176"
     CDS             3431..4738
                     /gene="trmFO"
                     /locus_tag="P6L01_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HDP3031226.1"
                     /translation="MTQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHT
                     DKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFS
                     GYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDA
                     AAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEK
                     EKYFEGCMPFEVMAERGRKTLLFGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNI
                     VGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQ
                     FAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKN
                     FQPMNANFGLLPSLETRIKDKKERYEAQANRALDYLENFKKTL"
BASE COUNT         1925 a          721 c          912 g         1454 t
ORIGIN      
        1 ttgaaatttt aaattctttc caacatactt ttcacttttt aagtcagtat ttttactcct
       61 taaaataaca ttacatcgac aatgtttttg tattagaaat gtaaaacaaa aatagaatat
      121 gattttgtat gattacaaaa aggagttgat ttattgatta aactattttt gcttaagtta
      181 tactgggcac acttttcgac taaacaaatt catcaatttt taatggcata tcctaatgta
      241 attaaagagg agggaagaaa aaaagatagt tatttatgtg aatgggtgaa tagggaagaa
      301 aatgttcttt tattacgtaa atactatgct tttataaaac ttgatcataa cgatattatt
      361 aaagaactgc agaaattaaa agtaagttac attacatata tggattctga atacccagtg
      421 ctattaaaag aaatatatca atttccatta cttcttttct ataaagggaa catcaaatta
      481 ataaataata tgcatcattt ggcagtagta ggtgcaagag attctacaag ttatacccaa
      541 cagtctttag aatttttatt atcaaatgat aaaagcaaat atttaacaat tgtttccggc
      601 cttgctcaag gagctgatgc aatggcacat caaatagctt taaaatacaa tctccctaca
      661 attgcagttt tagcctttgg ccatcaaaca cattatccca aaagtacatt agcattaaga
      721 aataaaatag aagaaaaagg tttagttata tccgaatatc caccacatac accaattgct
      781 aaatatagat ttcctgagcg caatagaatt atcagcggtt tgtcaaaagg ggttttaatt
      841 actgaggcta aggaacaaag tggcagtcac atcacgatag attttgcatt agagcaaaat
      901 agaaatgttt atgttttacc tggatctatg tttaatccta tgacaaaagg taatttatta
      961 cgtatccaag aaggtgctaa ggtagtatta aacgctaatg atatatttga agactactat
     1021 atttaaaact aaaaataacg agttgcttca tatttctttt gttacccttt gtaatttatt
     1081 ttttaataaa atctataaaa aatagacagg gaaaatgatt tgtttagata taaaacgttg
     1141 acaaaagcaa aattaagcgt ttatcattta tctttagtaa ttagattagc gagggggaaa
     1201 tgacattggc agataattta gtcattgttg aatcgcctgc aaaagcaaaa accattgaaa
     1261 agtatttagg taagaaatat aaagttatag cttcaatggg acacgtcaga gacttaccaa
     1321 gaagtcaaat gggtgtcgac actgaagata attacgaacc aaaatatata acaatacgcg
     1381 gaaaaggtcc tgttgtaaaa gaattgaaaa aacatgcaaa aaaagcgaaa aacgtctttc
     1441 tcgcaagtga ccccgaccgt gaaggtgaag caattgcttg gcatttatca aaaattttag
     1501 agcttgaaga ttctaaagaa aatcgcgttg ttttcaacga aataactaaa gacgctgtta
     1561 aagaaagttt taaaaatcct agagaaattg aaatgaactt agtcgatgca caacaagcgc
     1621 gtcgaatatt agatagattg gttggctata acatctcgcc agttctatgg aaaaaagtga
     1681 aaaaaggttt gtcagcgggt cgagttcaat ctgttgcact tcgtttagtc attgaccgtg
     1741 aaaatgaaat tcgaaacttt aaaccagaag aatattggac tattgaagga gaatttagat
     1801 acaaaaaatc aaaattcaat gctaaattcc ttcattataa aaataaacct tttaaattaa
     1861 aaacgaaaaa agatgttgag aaaattacag ctgcactaga tggagatcaa ttcgaaatta
     1921 caaacgtgac taaaaaagaa aaaacgcgta atccagcaaa cccatttaca acttctacat
     1981 tacaacaaga ggcggcacgt aaattaaact ttaaagcaag aaaaacaatg atggtcgcac
     2041 aacaattata tgaaggtata gatttgaaaa aacaaggtac gattggttta ataacatata
     2101 tgagaaccga ttctacacgt atttcagata ctgccaaagc tgaagcaaaa cagtatataa
     2161 ctgataaata cggtgaatct tacacttcta aacgtaaagc atcagggaaa caaggtgacc
     2221 aagatgccca tgaggctatt agaccttcaa gtactatgcg tacgccagat gatatgaagt
     2281 catttttgac gaaagaccaa taccgattat acaaattaat ttgggaacga tttgttgcta
     2341 gtcaaatggc tccagcaata cttgatacag tctcattaga cataacacaa ggtgacatta
     2401 aatttagagc gaatggtcaa acaatcaagt ttaaaggatt tatgacactt tatgtagaaa
     2461 ctaaagatga tagtgatagc gaaaaggaaa ataaactgcc taaattagag caaggtgata
     2521 aagtcacagc aactcaaatt gaaccagctc aacactatac acaaccacct ccaagatata
     2581 ctgaggcgag attagtaaaa acactagaag aattgaaaat tgggcgacca tcaacttatg
     2641 caccgacaat agatacgatt caaaagcgta actatgtcaa attagaaagt aagcgttttg
     2701 ttcctactga gttgggagaa atagttcatg aacaagtgaa agaatacttc ccagagatta
     2761 ttgatgtgga attcacagtg aatatggaaa cgttacttga taagattgca gaaggcgaca
     2821 ttacatggag aaaagtaata gacggtttct ttagtagctt taaacaagat gttgaacgtg
     2881 ctgaagaaga gatggaaaag attgaaatca aagatgagcc agccggtgaa gactgtgaag
     2941 tttgtggttc tcctatggtt ataaaaatgg gacgctatgg taagttcatg gcttgctcaa
     3001 acttcccgga ttgtcgtaat acaaaagcga tagttaagtc tattggtgtt aaatgtccaa
     3061 aatgtaatga tggtgacgtc gtagaaagaa aatctaaaaa gaatcgtgtc ttttatggat
     3121 gttcgaaata tcctgaatgc gactttatct cttgggataa gccgattgga agagattgtc
     3181 caaaatgtaa ccaatatctt gttgaaaata aaaaaggcaa gacaacacaa gtaatatgtt
     3241 caaattgcga ttataaagag gcagcgcaga aataatattt ttattcccta gagacatttt
     3301 aagattgtta aatagaatca ttagtgaatc ttattttaaa gatagtaatg gattaatcta
     3361 aataagtgcg gataatataa acataacaac ataattaaaa gacataaatg acaataaaag
     3421 gagtatagaa atgactcaaa ctgtaaatgt aataggtgct ggtcttgccg gttcagaagc
     3481 ggcatatcaa ttagctgaaa gaggaattaa agttaatcta atagagatga gacctgttaa
     3541 acaaacacca gcgcaccata ctgataaatt tgcggaactt gtatgttcca attcattacg
     3601 cggaaatgct ttaactaatg gtgtgggtgt tttaaaagaa gaaatgagaa gattgaattc
     3661 tataattatt gaagcggctg ataaggcacg agttccagct ggtggtgcat tagcagttga
     3721 tagacacgat ttttcaggtt atattactga aacacttaaa aatcatgaaa atatcacagt
     3781 tattaatgaa gaaattaatg ccattccaga tggatacaca attatcgcaa caggaccact
     3841 tactacagaa acccttgcgc aagaaatagt ggacattact ggtaaagatc aactttattt
     3901 ctatgatgca gctgctccaa ttattgaaaa agaatctatt gatatggata aagtttactt
     3961 aaagtcccgt tatgataaag gtgaagctgc atatttaaac tgtcctatga ctgaggatga
     4021 atttaatcgc ttttatgatg cagtattaga agctgaagtt gcacctgtaa attcatttga
     4081 aaaagaaaaa tatttcgagg gttgtatgcc ttttgaagta atggcagaac gcgggcgcaa
     4141 gacattacta tttggaccaa tgaaaccagt aggattagaa gatccaaaga ctgggaaacg
     4201 tccttatgca gtggttcaat taagacaaga tgacgctgct ggcacactct acaatattgt
     4261 cggcttccaa acacatttaa aatggggagc tcaaaaagaa gtcattaaat taattccagg
     4321 tttagagaat gttgatattg ttagatatgg tgtgatgcat agaaatacct tcattaactc
     4381 accggacgta ttaaacgaaa aatatgaatt gatttcacaa ccaaacatac agtttgcggg
     4441 acaaatgact ggtgttgaag gttatgtaga aagcgcagct agcggcttag ttgcaggtat
     4501 caatcttgcg cataaaatat taggcaaagg tgaggtagta tttccgagag aaactatgat
     4561 tggaagtatg gcttactata tttctcatgc taaaaacaat aagaatttcc aacctatgaa
     4621 tgctaacttc gggttattac catctttaga aactagaatt aaagataaaa aagaacgcta
     4681 tgaagcacaa gctaatagag ctttggatta cttagaaaat ttcaaaaaaa ctttataaaa
     4741 tagttagaaa gactagatat gctattcatt cttaagtcat caacgagtaa gtaatgactt
     4801 tctaaatgga aaatacttat cctagtcttt ttaattttgg aattgttacg tatttctgac
     4861 aatttagaat tcgtattcaa aaaaatatct aaataaataa cacgcaataa gttgattgat
     4921 gtaacatgta agagaatgtt ttaaataaac tttatttaaa agacaatgaa ataataaatg
     4981 gcaaggctat taataaagac ttttagtaat ta
//
LOCUS       DAOBBH010000169         4996 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.169, whole genome shotgun sequence.
ACCESSION   DAOBBH010000169 DAOBBH010000000
VERSION     DAOBBH010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.169"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..714
                     /gene="dapD"
                     /locus_tag="P6L01_002177"
     CDS             <1..714
                     /gene="dapD"
                     /locus_tag="P6L01_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDP3031227.1"
                     /translation="QHLTAEEIIQYISDAKKSTPIKVYLNGNFEGITYPESFKVFGSE
                     QSKVIFCEADDWKPFYEAYGSQFEDIEIEMDRRNSAIPLKDLTNTNARIEPGAFIREQ
                     AIIEDGAVVMMGATINIGAVVGEGTMIDMNATLGGRATTGKNVHVGAGAVLAGVIEPP
                     SASPVIIEDDVLIGANAVILEGVRVGKGAIVAAGAIVTQDVPAGAVVAGTPAKVIKQA
                     SEVQDTKKEIVAALRKLND"
     gene            857..2008
                     /locus_tag="P6L01_002178"
     CDS             857..2008
                     /locus_tag="P6L01_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDP3031228.1"
                     /translation="MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDC
                     PLETGVIAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTALM
                     LFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGVFDKYPIEAVFGIHVNPF
                     ADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQAHSCGEALQHVLTQISQIQQFH
                     LNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMHKIAESVKLL
                     FNVECEVKFAEGYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLA
                     PAYFVFIGTRNEDKGFVTGLHTSHLNFDEKVLINVVNFYENLLNNYKEV"
     gene            2013..3098
                     /locus_tag="P6L01_002179"
     CDS             2013..3098
                     /locus_tag="P6L01_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDP3031229.1"
                     /translation="MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQ
                     FIHAGIDTFSTTSLREAIQIRQLAPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYN
                     HKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYADE
                     FDVSDYNVERSQWMEIVEALLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIAL
                     YGSRPYSSLNQHDIVQSLTVKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGY
                     GDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTF
                     KGVGANSEQLSAMNHDSLKKEYISNDC"
     gene            3088..4353
                     /gene="lysA"
                     /locus_tag="P6L01_002180"
     CDS             3088..4353
                     /gene="lysA"
                     /locus_tag="P6L01_002180"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDP3031230.1"
                     /translation="MTVKYNQNGELTMDGISLKTIAQSFGTPTIVYDELQIREQMRRY
                     HRAFKDSGLKYNISYASKAFTCIQMVKLVAEEDLQLDVVSEGELYTALEAGFEPSRIH
                     FHGNNKTKHEIRYALENNIGYFVIDSLEEIELIDRYANDTVQVVLRVNPGVEAHTHEF
                     IQTGQEDSKFGLSIQYGLAKKAIDKVQQSKHLKLKGVHCHIGSQIEGTEAFIETAKIV
                     LRWLKEQGIQVELLNLGGGFGIKYVEGDESFPIESGIKDITDAIKSEIKVLGIDAPEI
                     GIEPGRSIVGEAGVTLYEVGTIKEIPEINKYVSIDGGMSDHIRTALYDAKYQALLVNR
                     NEEADDSVTIAGKLCESGDIIIKDAKLPSSVKRGDYLAILSTGAYHYSMASNYNQMQK
                     PSVFFLKDGKAREVIKRQSLRQLIINDTK"
     gene            complement(4593..4994)
                     /gene="msa"
                     /locus_tag="P6L01_002181"
     CDS             complement(4593..4994)
                     /gene="msa"
                     /locus_tag="P6L01_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarA expression modulator protein Msa"
                     /protein_id="HDP3031231.1"
                     /translation="MKYLILSLVANLLVFGVLSAIGLNINILAAMMIVLVIPIMISGI
                     LFFKTNIDKTYIFFNIIFIDFYYYIYNVHLMTLPKFNNYIKAEMMELEDIDVLITSKD
                     FGFDEILFYTLYLLLILIVLYYLKKQVKHKI"
BASE COUNT         1785 a          754 c          904 g         1553 t
ORIGIN      
        1 caacatttaa cagctgaaga aattattcaa tatataagtg atgctaaaaa gtctacacca
       61 ataaaagtat atttaaatgg taattttgaa ggcatcacat acccagaaag ttttaaagta
      121 tttggttcag aacaatctaa agtaatcttt tgtgaagcgg atgattggaa acctttttac
      181 gaagcatatg gtagtcaatt cgaagatata gaaattgaaa tggatcgtcg caattctgcc
      241 attccattaa aagacttaac aaatacgaat gcacgtattg aaccaggtgc atttattaga
      301 gaacaagcca ttattgaaga tggtgctgtc gttatgatgg gcgcaacaat taatattggc
      361 gcagtcgttg gcgaaggtac aatgattgat atgaatgcta ctctcggtgg tcgtgctaca
      421 actggtaaaa atgtacatgt aggggctggc gcagtattgg caggtgtgat tgaaccccct
      481 agtgcatcac cagttataat cgaggatgat gtattaatcg gtgcaaatgc agttatttta
      541 gaaggtgtac gtgttggtaa aggtgctatt gttgcagctg gcgcgattgt gacacaagat
      601 gtaccagctg gtgcagttgt tgctggtaca cctgcaaaag tgattaagca agcttctgaa
      661 gtacaagata ctaaaaaaga gattgtagca gcattaagaa aactgaatga ctagtgacgt
      721 caacgtataa taatttcaag gttgagatac gattatgtct caaccttatt gtttatcatt
      781 gaataaactt atgatcacaa atttcgaaat acgttacact atttatatag taatttaaca
      841 caaaggattg ataattatga atgaattaga atttgttacg aaacatcgcc gtcatttaca
      901 tcaacatcct gaattaagct tacatgaatt tgaaacaact gcttatatta aagcgttttt
      961 agatagttta aatattaaat acgattgccc attggaaact ggcgtcattg catacttaga
     1021 aggtaatggc tcacatacga tagcgtatag agctgatatt gatgcgttac ctattttaga
     1081 ggaaaatgat gtgccttatc gcagtcaatc tgatcatgtg atgcatgctt gtggacatga
     1141 tggtcataca actgcattaa tgctttttgt acaacgttgc aaagacatgc aagacgcagg
     1201 tcaattaccg caaaatgtcg ttttcatttt ccaacctgca gaagaaactg gtggcggtgc
     1261 aaatcgatta ataaaagccg gtgtctttga taagtatcca attgaagcgg tatttggtat
     1321 tcatgttaac ccatttgctg atgaaggcat tgcagtgata agagatgaag aaattacggc
     1381 cagcgcaaca gagtatcgct ttttcttaac aggcctgtca agtcatgttg ctgataaaga
     1441 acaagctcat tcttgtggtg aagcattaca acatgtatta actcaaatat cacaaattca
     1501 acaatttcac cttaacggtt tgaaacgaaa tattgttcat attggtcatt ttaaagctgg
     1561 tgaagcgatt aacactgtac caagtaatgg ttatttagaa ggtacaattc gtacatatga
     1621 tattgatgat ttaacaatcg ttaaaaatca aatgcacaag atagcagaaa gtgtcaagct
     1681 tctgtttaat gtagaatgtg aagttaaatt tgcagaaggt tatcccccta caatcaatag
     1741 tccgaaatta cgtactcaaa tagaggacgc cttaataaaa gctgatttaa atgtctatga
     1801 caaaccaacg ccattcttat ttggggaaga ttttagtttt tatggtcaac aactagctcc
     1861 agcttacttt gtttttatag gaacacgaaa tgaagataaa ggttttgtaa ctggtttgca
     1921 cacatcacat ttaaattttg atgaaaaagt gttaataaac gtggttaatt tttacgaaaa
     1981 tttattaaat aattacaaag aggtgtaata cattgacagc aacatggtct gtaaataaga
     2041 aaatattttt acaaaatgca atcacagtca aaaacaatca gccattaatg gcagttgtta
     2101 aaaataatgc atatcactat gacctagaat ttgctgtaac tcagtttatc catgcaggta
     2161 tagatacatt tagcacaaca tcactacgag aagcaattca aattagacaa cttgctccag
     2221 atgcaacaat ctttttaatg aatgcagttt acgagtttga tttagtccgt gaacatcaaa
     2281 tacacatgac tttgccgtcg ttgacatatt actataacca taaaaatgat ttagcaggta
     2341 ttcatgttca cttagaattt gaaaatttat tacatcggtc tggatttaaa gatttaaacg
     2401 aaattaaaga agtattgaaa gatcaccatc ataatcaaaa tgcaaaaatg attattagtg
     2461 gtttatggac ccattttgga tatgctgatg aattcgatgt gtcagattat aatgttgaac
     2521 gttcacaatg gatggaaatt gttgaagcac ttttatctga aggttatcag ttcgacctaa
     2581 tccatgctca aaatagtgcg agtttttatc gggaaggaca aatattacta ccccaccata
     2641 cacatgcgcg tgtaggtatt gcgttatacg gttcaagacc atatagttca ctgaatcaac
     2701 atgatatagt tcagtcatta actgtaaaag cacatgttat tcaagtgcgc gaagtacaag
     2761 ctggtgatta ttgcggttat agctttgcct ttgaagtgac taaaaacaat acaaaattag
     2821 ctgtagttga tatcggttat ggcgatggaa ttttaagaac tcgtgctaaa catgaagcac
     2881 ttatcaatgg taaacgctac ccgatacgtg cattaatgat gagccatatg tttgttgaag
     2941 tagatggcaa tgtacatgca caagatgaag ttattcttta taataatgat atccgcatcg
     3001 atgaatatac ctttaaaggt gttggtgcaa attctgaaca attaagtgct atgaatcatg
     3061 attctttaaa aaaggagtac atttcaaatg actgttaaat ataatcaaaa tggcgaatta
     3121 acaatggatg gtattagttt aaaaacgatt gcacaaagct ttggtacacc taccattgtt
     3181 tatgatgaac tacaaattag agaacagatg cgccgttacc atcgcgcatt taaagatagt
     3241 ggattaaaat acaatatttc atacgcctca aaggcattta cttgcattca aatggtcaaa
     3301 cttgtagctg aggaagattt acagttagat gttgtttctg aaggtgaatt atatacagct
     3361 ttagaagcag gttttgaacc gagtcgcatc catttccatg gtaacaataa aacgaaacat
     3421 gaaattaggt atgctttaga aaataatatc ggttattttg ttatagattc attagaagaa
     3481 attgaattaa tagaccgcta tgctaatgat acggttcaag ttgtattacg agttaatcca
     3541 ggtgttgaag cacatacaca cgaatttatt caaactgggc aagaagatag taagtttgga
     3601 ttatcaattc aatatggctt agctaaaaaa gcaattgaca aagtccaaca atctaaacac
     3661 ttaaaattaa aaggtgtaca ttgtcatatt ggttcacaga ttgaaggtac agaagcattt
     3721 attgaaactg ctaaaattgt tttacgttgg cttaaagagc aaggcattca agttgaatta
     3781 ttaaaccttg gtggtggctt tggtattaaa tatgttgaag gtgacgaaag tttccctatc
     3841 gaaagtggta ttaaagatat tacagacgca ataaaatccg aaattaaagt tctaggtata
     3901 gatgcaccag aaataggtat tgaaccggga cgatcaattg taggtgaagc tggcgttact
     3961 ttatatgaag ttggaaccat taaagaaatt ccagagatta ataaatatgt ttcaatcgat
     4021 ggcggtatga gtgatcatat cagaactgca ctttatgacg caaagtatca agcattgctt
     4081 gttaatagaa atgaagaagc agatgacagt gtaactatag ctggaaaatt atgtgagtct
     4141 ggtgatatca ttattaaaga cgctaaatta ccttcatcag tcaaacgtgg agactatctt
     4201 gctatattat caactggtgc atatcattac tctatggcat ccaattacaa tcaaatgcaa
     4261 aagccttctg tgtttttctt aaaagatggc aaagcacgtg aagttataaa gcgacaatcg
     4321 ttaagacaac tcattattaa tgatacaaaa taaaaataaa caaagtaatc cccgaagcac
     4381 agaaaattat actgtgactc ggggaatttt tatgcattat agatgactgt agaaaacatg
     4441 cttggacata tactctaaaa aaggaagaac agcacatttt tagttagaaa gtatttattt
     4501 ctttttgtca ttaatccttt atatttagtt agccctattc aactaattta aagcatcata
     4561 tacataccac actttaaaca agcatcatga aattagattt tatgctttac ttgctttttc
     4621 aaataatata aaacgataag aattaacaac aaatataatg tataaaacag aatttcatca
     4681 aatccaaaat ctttacttgt aattaataca tcaatatctt ctaattccat catctctgct
     4741 ttaatataat tattaaactt tggcaaagtc attaaatgta cattgtaaat atagtaataa
     4801 aagtcgataa atatgatgtt aaaaaatatg tatgttttat caatattagt tttgaaaaat
     4861 agtatcccag aaatcattat cggaatcact aatacaatca tcattgctgc taaaatatta
     4921 atatttaatc caattgcaga taatacaccg aaaactaata aattagcaac taaactaagg
     4981 ataagatatt tcataa
//
LOCUS       DAOBBH010000170         4989 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.170, whole genome shotgun sequence.
ACCESSION   DAOBBH010000170 DAOBBH010000000
VERSION     DAOBBH010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.170"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            50..736
                     /gene="radC"
                     /locus_tag="P6L01_002182"
     CDS             50..736
                     /gene="radC"
                     /locus_tag="P6L01_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDP3031232.1"
                     /translation="MKIKEMVTSEMPRERLLSHGAKSLSNTELLAILINTGRKGFSSI
                     DISNELLKSASNLNELKKSSINDLIKVKGIGLQKAITLKAAFELGERMGRRAENNRIK
                     ITQPSDVADYMIPTMKDLTQEHFVILLLNSKNVVIKETCVFKGTLNSSIVHPREIFSI
                     AVRENANAIIAVHNHPSGDVTPSQEDIITTMRLKECGLILGIDLLDHIIIGDNRFTSL
                     VEAGYFDEND"
     gene            804..1088
                     /locus_tag="P6L01_002183"
     CDS             804..1088
                     /locus_tag="P6L01_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031233.1"
                     /translation="MTLFIIIGVLVPMVYTMQLNIKNEPVTKRNLLITLALSTLGILV
                     TALAGVIVTKQAFPLLSVAIGSIFTGIVWGLLLSGSYALIRFLSNAFGRK"
     gene            complement(1205..1402)
                     /locus_tag="P6L01_002184"
     CDS             complement(1205..1402)
                     /locus_tag="P6L01_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031234.1"
                     /translation="MSILTIILIALLVILLFRVGLSILRFLIYVGLVLLCIYLGYQGL
                     IWLLDFFQINSGFLPHFQFNN"
     gene            1562..2035
                     /locus_tag="P6L01_002185"
     CDS             1562..2035
                     /locus_tag="P6L01_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4930 family protein"
                     /protein_id="HDP3031235.1"
                     /translation="MRLIFSMIKNIIAVIAIVIIVYIALKYAPFLKEQDWNPVNHDND
                     QMNQVTQPTNDAQHVYVSGEKYSLKENDLIKNVPLSQIKNVFKMIDKQEFMAVSGMNR
                     MAYNDQYIIGQRGDEFILYKFGDESMRVYNTEFEMQQDLNELGQNLQLKPENAYQ"
     gene            2109..2228
                     /locus_tag="P6L01_002186"
     CDS             2109..2228
                     /locus_tag="P6L01_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031236.1"
                     /translation="MAEQSKQKQANEQQKAQNLFARWRQLQNSNSESSNDTNK"
     gene            2427..3269
                     /gene="mreC"
                     /locus_tag="P6L01_002187"
     CDS             2427..3269
                     /gene="mreC"
                     /locus_tag="P6L01_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDP3031237.1"
                     /translation="MLKFFKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSV
                     SFGQRVVSYPVNFVAGTIGDFFKKGDSKESKNKISQLESKNQQLEAENEKLKKELDLK
                     DISKFDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSQGFVGRVTKVNKFSS
                     QVDLISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVVT
                     SGLADQLPSNLYIGEVTKVQNDQYGLAKEVRVKTGADLTDLSHVYVAKRDPKTIPDDE
                     SRDK"
     gene            3269..3799
                     /gene="mreD"
                     /locus_tag="P6L01_002188"
     CDS             3269..3799
                     /gene="mreD"
                     /locus_tag="P6L01_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDP3031238.1"
                     /translation="MRTLYYFLIGILLFYIDTAIGLLIPMHIGKFELVFVPHLTFMYI
                     LMMVVYRGFGVSLLLSIFLGVMTDVYFGSIYGVYLFGYILFLALIDRFFKIFYKDHSM
                     LFIIILASTLLLEVYVALIYGMLGFIQFDIIHFVVFRLLPTLIMNFVLLIMLYPLIIK
                     FLKKTNNDIDMKRRQW"
     misc_feature    3826..4022
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4035..4343
                     /gene="rplU"
                     /locus_tag="P6L01_002189"
     CDS             4035..4343
                     /gene="rplU"
                     /locus_tag="P6L01_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDP3031239.1"
                     /translation="MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGD
                     SVKVGAPTVEGATVTATVNKQGRGKKITVFTYKRRKNSKRKKGHRQPYTKLTIDKINA
                     "
     gene            4349..4669
                     /locus_tag="P6L01_002190"
     CDS             4349..4669
                     /locus_tag="P6L01_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDP3031240.1"
                     /translation="MITVDITVNDEGKVTDVIMDGHADHGEYGHDIVCAGASAVLFGS
                     VNAIIGLTSERPDINYDDNGGHFHIRSVDTNNDEAQLILQTMLVSLQTIEEEYNENIR
                     LNYK"
     gene            4681..4965
                     /gene="rpmA"
                     /locus_tag="P6L01_002191"
     CDS             4681..4965
                     /gene="rpmA"
                     /locus_tag="P6L01_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDP3031241.1"
                     /translation="MLKLNLQFFASKKGVSSTKNGRDSESKRLGAKRADGQFVTGGSI
                     LYRQRGTKIYPGENVGRGGDDTLFAKIDGVVKFERKGRDKKQVSVYAVAE"
BASE COUNT         1812 a          664 c          920 g         1593 t
ORIGIN      
        1 attcactttt ttataatgat atccatcaat ttttaggagg cgtatatttt tgaaaattaa
       61 agaaatggta acttcagaaa tgccaagaga acgtttgtta agccatggtg caaaaagtct
      121 ttcgaataca gaattattag ctatattaat taacaccgga agaaaaggat tctcgagcat
      181 agacattagt aatgaactgc ttaaatctgc ttcgaatctg aatgaattga aaaaatcttc
      241 aattaacgat ttgataaaag ttaaagggat tggattacaa aaagcaatta ctttaaaagc
      301 agcatttgag ttaggggaaa ggatggggag aagagctgaa aataatcgta taaaaataac
      361 gcaaccaagt gatgttgctg attatatgat tccaacaatg aaagatttaa cacaagaaca
      421 ttttgtcatt ttattgttaa attcaaaaaa tgtagtgatt aaagaaacct gtgtttttaa
      481 aggtacatta aatagttcga ttgtacatcc acgtgaaatt tttagtattg cggtgagaga
      541 aaatgccaat gcaatcatcg cagttcataa tcatccatcc ggtgatgtaa cgccctcaca
      601 agaagatatc ataacaacaa tgaggttgaa ggagtgtggt ttgattttag ggatagattt
      661 attggatcat attataatcg gtgataatag atttaccagt cttgtagaag cgggttactt
      721 tgatgaaaat gattgaagtt gatagaatta atgacgtatc ttgtgtataa tacctacgaa
      781 gtactttcat tggaggaaaa atagtgactc tatttattat tatcggggtt ctcgtgccaa
      841 tggtttatac catgcagtta aatattaaaa atgaacctgt aacaaagcgc aatctattaa
      901 taacattagc tttatctacg ttaggtattt tagtaacggc gttagcaggt gtgatcgtta
      961 cgaaacaagc ttttccttta ttaagtgtag caatcggctc aatttttact ggaatcgttt
     1021 ggggcctttt actaagtggt agctacgcgc tgatacgatt tttatctaac gcatttgggc
     1081 gtaagtaata gtaatctgtt catcaagtag tatccgtgct tgaaaccaaa ttaaaactcc
     1141 taatgtggaa ctagttaaaa aattctaaac tagtacatta ggagttttgt tatgcagaat
     1201 aaatttaatt gttaaattga aagtgcggta aaaacccact atttatttga aaaaaatcga
     1261 gcaaccaaat taaaccttga tatcctaagt aaatacataa taaaacaagt ccgacataaa
     1321 ttaaaaatcg caaaatagaa agtccaactc taaaaaggag gatgactaat agtgctatta
     1381 aaataattgt tagtatactc aatgctacaa acctcctcat acgcttttaa atcataacaa
     1441 aattataagc tattttaaat gaacgtgcat cagtcctaag atatatcatg attaaggctg
     1501 aagactgagg aaaatataca taataaaata tataattaga tgaagacatt ggaggcgaac
     1561 tatgagattg atatttagta tgattaaaaa cattatagca gtaatagcaa ttgtgataat
     1621 tgtttatatc gccttgaaat acgcaccttt tttaaaagaa caagattgga atcctgtaaa
     1681 tcacgataat gatcaaatga atcaagttac gcaacccact aatgatgccc aacatgttta
     1741 tgtaagtggt gagaaatatt cattaaaaga aaatgattta ataaagaatg taccattaag
     1801 ccaaattaaa aatgtattta aaatgataga taaacaagag tttatggctg tgtctggaat
     1861 gaatcgcatg gcttataatg atcaatatat tataggtcaa agaggagacg aatttattct
     1921 ttataaattt ggagatgagt caatgcgtgt ttacaatact gaatttgaaa tgcaacaaga
     1981 cttaaatgaa ttagggcaaa atttacaact aaaacccgaa aatgcttatc aataggaaat
     2041 tattgcataa gaaatatatt ttagttatcc tgtcactaga ataaatttaa aaggttggtg
     2101 tagttcaaat ggcagaacaa tcaaaacaga aacaagctaa tgaacaacaa aaggcccaga
     2161 acttattcgc acgttggaga caacttcaga attcgaacag tgaatcctcg aacgatacaa
     2221 acaaataatt ttaaaattta aatcgattat ttaaactact agtgactgga atgtttgcta
     2281 gtagtttttt gtttttaaaa tgccacaaat tttttacgga gttgttcatt ttcaaagtaa
     2341 ttcgtagtta gtgctttatt tcataatggg agtaatataa aatagaaaag gatatacaat
     2401 attttgacat aatagaggtg ttctgggtgc ttaagttttt taaaaataac aaattaattg
     2461 ttgttttatg tgcaattatc gtttttattg cattaattgg gctgtccata cgttcacaat
     2521 ctcaatcacc tcctgaacaa tatataggtg attctgtgtc ttttggacaa cgagttgtga
     2581 gttatccagt taattttgtt gctggtacga ttggggactt ttttaaaaaa ggagattcta
     2641 aagaatctaa aaataagatt agccagttag aatctaagaa ccaacaatta gaagcggaaa
     2701 atgaaaaatt aaaaaaagag cttgatttaa aagatatttc aaaatttgat cctatttcta
     2761 ctacggtttt agcaagaaat ccggatcagt ggatgaatac aattgtaatt gataagggat
     2821 ctaaatcagg tataacttca aatatggctg tgatgacatc acaaggtttt gttggaagag
     2881 ttactaaagt taataaattt tcttcacaag ttgatttaat ctcaactaat acacgtgcgg
     2941 gtaaattatc tgtaaatata caacacggtt ctaaaaatat atttggttta attgatcgtt
     3001 atgatgaaaa gaactcagaa cttgtaatta gtgacattaa taatagagat aatatctcaa
     3061 aaggtgataa agtcgttaca agtggattag ctgatcaact accaagtaat ttatatatag
     3121 gagaagtgac taaggttcaa aatgatcaat acggcttagc taaagaagtt agggttaaga
     3181 ctggtgcaga cttaacagat ttgagtcatg tttatgttgc aaaaagagat cctaaaacaa
     3241 ttcctgatga tgaaagcagg gataaataat gcgtacactg tattattttt tgataggtat
     3301 tttactattt tatatagata ctgcaattgg gcttcttatt ccaatgcata taggtaagtt
     3361 tgaacttgta tttgtaccac accttacatt tatgtatatt ttaatgatgg tagtgtatcg
     3421 tggctttggc gtatcattat tgctcagcat atttttaggc gtaatgactg atgtatactt
     3481 tggtagtatt tatggagtgt acttatttgg ctatatattg tttttagcac ttatagatcg
     3541 attctttaaa attttttaca aagatcattc gatgttattc attattatat tagccagcac
     3601 cttattatta gaagtctatg tggcattaat atacggtatg ttaggattca ttcaatttga
     3661 tattattcat tttgtagtct ttagattatt gccaacatta attatgaatt ttgtactgtt
     3721 gattatgctt tatccgttga ttataaagtt ccttaaaaaa acaaacaatg acattgacat
     3781 gaaacgtcgt caatggtaag atgcaatagt tgagtagtta taagtagcta catacaaccg
     3841 ctcaaatata ggttcaagaa catttttaat gaagaaagcg attgtcaatc aaggcgttga
     3901 aggaagaaac gagaagagtt tatgtttata aatgactcga gttgcgaccg aaacaacgta
     3961 gagtgcaaac gcttgactca ataacgaaat gtcacctatg tatggcgtga cttattatac
     4021 aggaggtgca aagtatgttt gctattattg aaacaggtgg aaaacaaatc aaagtagaag
     4081 aaggtcaaga aatcttcgtt gaaaaattag acgtaaacga aggagatact tttacatttg
     4141 ataaagtatt atttgtaggt ggagattcag ttaaagttgg agcgccaaca gttgaaggtg
     4201 caacagttac tgctactgtt aataaacaag gtcgcggtaa aaaaatcact gtattcacat
     4261 acaaacgtcg taaaaattca aaacgtaaaa aaggccatcg tcaaccatac actaaattaa
     4321 caatcgataa aatcaacgcg taattattat gattactgtt gatattacag ttaatgatga
     4381 aggcaaagta acagacgtta ttatggatgg ccatgctgac catggtgaat atggtcatga
     4441 tatcgtttgt gctggagctt cagctgtatt gtttggtagt gttaatgcga ttataggatt
     4501 gacatctgag agaccagata tcaattatga cgacaatggt ggtcattttc atataagaag
     4561 cgttgataca aataacgatg aagcgcaact aattcttcaa acaatgcttg tgtctttaca
     4621 aactattgaa gaagaatata atgagaatat tagattaaat tataagtgag gtgcattccg
     4681 atgttaaaat taaacttaca attcttcgca tctaaaaaag gggtaagttc tacaaaaaac
     4741 ggacgtgact ctgaatcaaa acgcttaggt gctaaacgtg ctgacggtca attcgtaaca
     4801 ggtggttcaa ttttatatcg ccaacgtggt actaaaattt accctggtga aaatgtaggt
     4861 cgtggtggcg atgatacatt attcgctaaa atcgacggcg ttgttaaatt cgaacgtaaa
     4921 ggtcgcgaca aaaaacaagt ttctgtatat gcagtagctg aataattttg tctagttaac
     4981 accagaagt
//
LOCUS       DAOBBH010000171         4965 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.171, whole genome shotgun sequence.
ACCESSION   DAOBBH010000171 DAOBBH010000000
VERSION     DAOBBH010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.171"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(355..1359)
                     /locus_tag="P6L01_002192"
     CDS             complement(355..1359)
                     /locus_tag="P6L01_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HDP3031242.1"
                     /translation="MSIIIEKSGLFSSFQDFGRRGYEHDGVIPCGALDTLAHEIANRL
                     VANDKNEATLEMTNKMATIRFTEPTLIALAGGNVKAYTEHMTISPYKLYLLDKGDVLK
                     FRETSYTSRVYLAVGGGFELDAWLGSNSTDFNVKIGGFKGRTLQDGDEIKLKRDYTAR
                     HHKLFENLAHTKQTDWGIDGYALSFNYMSDVFHVVKNKGTEDFKEDAIQRFVKHDYKV
                     TSKANRMGMMLEGEKIKAFYEDMPPYQTVKKGTIQIKRDGTPIILLNDHYTLGSYPQI
                     GTIASYHLTKLAQKPQGSRLKFQFIDILTAEKNLVKYSNWLNQLFHGIEYRMQLEMMK
                     "
     gene            complement(1343..2053)
                     /locus_tag="P6L01_002193"
     CDS             complement(1343..2053)
                     /locus_tag="P6L01_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HDP3031243.1"
                     /translation="MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIE
                     IVPSESDMMIVYDARDMIKHHNIQSPFLYMKALIESIHLNIKHDFNQQDLIEIPIVYG
                     SKYGPDIESLLKHYKIKLETFIELHSKTQYFVSMMGYSPGFPYLTGLNKKLYINHTSK
                     QKKFIPAGSVVLEGKKCGIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFR
                     PINENELEVGAFKDVNHN"
     gene            complement(2141..2347)
                     /locus_tag="P6L01_002194"
     CDS             complement(2141..2347)
                     /locus_tag="P6L01_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031244.1"
                     /translation="MIIVYIVLLLILVYVNYRLVNRLLSENRIYVVRLIVTITTVISF
                     ILVYALIHELMPFVVRAMDLMYHQ"
     gene            complement(2413..3021)
                     /locus_tag="P6L01_002195"
     CDS             complement(2413..3021)
                     /locus_tag="P6L01_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDP3031245.1"
                     /translation="MQLFETMKIDNGHIPRLTYHTNRIKCSSERLNFKFDEHAWRNEL
                     NDVTTKYHSGQYRLKIVLNAESKFETIVSPLPEKSSFTAKFQVLPKVVNPTFIKNKTT
                     ERKHLAHNHETDLILLTSEDGKVLEFDIGNIVIEEDGKWYTPSYKDDFLKGCMRDYLI
                     DSDKLVEKDFNKNELIYKYHNNEIRLFLINSLREVADVHLCL"
     gene            complement(3021..4172)
                     /locus_tag="P6L01_002196"
     CDS             complement(3021..4172)
                     /locus_tag="P6L01_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HDP3031246.1"
                     /translation="MRIEYNYRYYLTENEYKQYHIQLKGFIKKYVATKLADVGEVIHF
                     AQAQQRQGRYVSLYLSYEAAKYFNHVMCTHSLAKDDIYAVAYSFEKAESINSTYEHQT
                     SYVSKHHFSFVESSEVMMTNIKRVQQAIVEGETYQVNYTARLTDNIYYPISTLYERLT
                     QFSNGNYTALLQTDEIQVASISPELFFQKGQFNNVDNVIISKPMKGTMPRGKTEAEDQ
                     QYYKTLQTSSKDRAENVMIVDLLRNDIGRISQSGSIKVYKLFFIEAYKTVFQMTSMVS
                     GTLKTNTDLTQILTSLFPCGSITGAPKLNTMKYIKQFESSPRGIYCGAIGLLLPTEDD
                     KMIFNIPIRTIEYKYGQAIYGVGAGITIDSKPKDEVNEFYAKTKILEML"
     gene            complement(4156..4749)
                     /locus_tag="P6L01_002197"
     CDS             complement(4156..4749)
                     /locus_tag="P6L01_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDP3031247.1"
                     /translation="MILVIDNNDSFTYNLIDYIKTQTKLTVQVVGIDNLLIEDVINMK
                     PKAIVISPGPGNPDDYPILNEVLEQFYQRVPILGVCLGFQCIVSYFGGNIIHGYHPVH
                     GHTTQLRHTNEGIFQGLPQNFNVMRYHSLIADGATFPNCLKITAKNDEAIIMAFEHIR
                     FPVFGVQYHPESILSEYGYRQVELFLSKVGDYCENRI"
BASE COUNT         1588 a          874 c          677 g         1826 t
ORIGIN      
        1 taattacttt cagattcaag tttataattt tcattacttg tagctttcaa ctttgattta
       61 acaagtattt tgggctacaa ctcatatttg cttgtgtaag tttctttatt taatattatc
      121 attcaattaa caaatttaaa atttgtaatc aaattgtaat aataagcata gctcaattaa
      181 taaatataga gtaattttag tcaaaattca agtaaaaaca cactttatct tttcatttta
      241 ctgtgaaacg aattagaaaa tgatacattt cgcagataaa acacagtatt atttcattat
      301 acattaggag gaaattattt gcatcggact cgagtatgag ctacgtacca aatattattt
      361 catcatttct aattgcattc tatattctat tccatggaat aattggttta accagttact
      421 atacttaaca aggttctttt cagccgttaa aatatctata aattgaaatt tcaaacgtga
      481 tccttgcggt ttttgtgcta atttcgttaa atgataactt gcgattgtac cgatttgcgg
      541 gtagctacct aacgtataat gatcatttaa taagataata ggtgtgccat cacgcttaat
      601 ttgtatcgtt ccttttttga cagtctgata cggcggcata tcttcataaa aagctttgat
      661 tttttcacct tcaagcatca ttcccatgcg atttgctttg ctcgttactt tataatcatg
      721 tttcacaaat ctttgaatgg catcttcttt aaaatcttcc gtacctttat ttttaacgac
      781 atgaaataca tcagacatat aattaaatga caaggcgtat ccatcaatac cccaatctgt
      841 ttgtttcgtg tgagcaaggt tttcaaataa cttatgatga cgagctgtat aatctctctt
      901 aagctttatt tcatcgccat cttgtaatgt tctaccttta aaaccaccaa tttttacatt
      961 aaagtcggtt gagttagatc ctaaccatgc atctaattca aatccgcctc ccacagctaa
     1021 atacactcgc gatgtataac ttgtttctct aaactttaaa acatcgcctt tatctaacaa
     1081 atacaattta tatggagata tagtcatatg ctcagtgtaa gctttgacat taccccctgc
     1141 taatgcaatc agcgtaggtt ctgtaaaacg aatcgttgcc attttattag tcatttccaa
     1201 agttgcttca ttcttgtcat tagcaactaa tcgattagca atttcatgtg ctaaagtatc
     1261 aagtgcacca catggaatta caccatcatg ttcatatccc cttctaccaa agtcctgaaa
     1321 gctactgaat aagccacttt tttcaattat gattgacatc tttaaacgct cctacttcta
     1381 attcattttc attgataggt ctaaatttaa tattatcgcc taactttaag cgtgcgaaat
     1441 ctgattcttt cggattaaaa agtgataatg gtgtataacc aataactaac caatcattaa
     1501 ttgtatccgt tgttacaata ccgcattttt tcccttcaag tactacagaa ccagctggaa
     1561 tgaatttttt ctgtttactc gtgtgattaa tatacaattt cttatttaat ccagttaaat
     1621 aaggaaaccc aggtgaatat cccatcatcg aaacaaaata ttgcgtctta gaatgtaatt
     1681 caataaaagt ttctagcttg attttgtaat gttttaaaag tgattctata tccggaccat
     1741 atttcgaacc atacacaatt ggtatttcaa tcaaatcttg ctggttaaaa tcatgtttta
     1801 tgtttaaatg aatcgattct attagtgctt tcatgtataa aaaaggtgat tgtatattat
     1861 ggtgtttaat catatccctt gcgtcataga caatcatcat gtctgattct gatggcacaa
     1921 tttctataat aaatggataa ttttgttcaa ttaaatatga gcgaagtgtt aataaatctt
     1981 cggttaaact tttagatact tctttttcaa ttgcgactac aatggcttgg tcaccttgac
     2041 tatatatttt catttgtaca cctcttcaat atttggttac tttactttca tttttaatta
     2101 tagaatcgat tgaatagttc gttttaccta ctcactaaat ttactggtgg tacattaaat
     2161 ccattgcccg cacaacaaaa ggcataagtt cgtgaattaa tgcgtataca aggataaagc
     2221 ttataacagt agtaattgtt actatcaaac gaacaacata tattctattt tcagatagca
     2281 atcgattcac taatcgataa tttacgtata caagaattaa caacagcaca atataaacaa
     2341 taatcatatt tggccccatt taatttttaa atttgttttc acatcatttt ctactttatt
     2401 atcatactaa ttttaaaggc aaaggtggac atcggcaacc tctcgtaaac tatttatcaa
     2461 aaataaacgt atctcattgt tatgatattt ataaatcaat tcgtttttat taaagtcttt
     2521 ttcaacaagt ttgtcactat ctattaaata atcacgcatg catcctttta agaaatcatc
     2581 tttataactt ggtgtgtacc attttccatc ctcttcaatg acaatgttgc caatatcaaa
     2641 ttcaaggacc ttgccgtcct ctgaagttag caatattaaa tctgtttcat gattgtgtgc
     2701 taaatgcttt cgttctgtcg ttttattttt tataaaagtt ggattaacta ctttgggcaa
     2761 cacttgaaat tttgctgtaa aactactttt ctcaggtaaa ggtgacacta tcgtttcaaa
     2821 tttgctttca gcatttaata cgattttaag tctatattga ccactgtgat actttgttgt
     2881 tacatcgttt aattcatttc gccatgcatg ttcatcaaat ttaaagttta atcgctcaga
     2941 agaacatttt atgcgattag tatgataagt aagtctaggg atatgtccat tatcaatttt
     3001 cattgtttca aataattgca ttataacatc tccaaaatct tggtttttgc gtaaaattca
     3061 tttacttcat cttttggctt agaatcaatt gtaatacctg ctccgactcc ataaatcgct
     3121 tgtccatatt tatactcaat agtacgaatc ggaatattaa aaatcatttt atcatcttca
     3181 gttggaagta atagtccaat tgctccgcag tatataccac gaggtgaact ttcaaattgt
     3241 ttaatatatt tcattgtatt cagtttcggt gcacctgtaa tcgaaccaca aggaaataac
     3301 gatgttaaaa tttgagttaa gtctgtatta gtttttaaag ttccacttac catcgaagtc
     3361 atttgaaata cagttttata tgcctcaata aaaaatagtt tatacacctt aattgagcca
     3421 ctctgtgata ttctccctat atcgtttctt agcaaatcaa caatcatgac attttctgca
     3481 cgatctttcg aagaagtttg caatgtttta taatactgtt gatcttcagc ttccgtttta
     3541 cctctaggca ttgtcccttt catcggtttg cttatgataa cgttatcgac attgttaaat
     3601 tgtccttttt gaaaaaataa ttctggtgag atagacgcta cttggatttc atcagtttgt
     3661 aataacgcag tataattacc attactaaat tgagttaatc gttcatataa agtactaata
     3721 ggataataaa tgttatccgt taagcgcgcc gtatagttca cttgatacgt ttcaccttca
     3781 acaattgctt gttggacacg tttaatatta gtcatcataa cctcagaaga ttcaacaaat
     3841 gaaaaatgat gctttgatac ataagaagtt tgatgttcat atgttgaatt tatgctttcc
     3901 gctttttcaa aactataagc tactgcataa atatcatctt tagctaatga atgtgtacac
     3961 ataacatgat taaaatactt tgccgcttcg taacttaaat ataacgagac atatctacct
     4021 tgtcgttgct gcgcttgtgc aaagtgtatc acttctccca catcagccaa cttagtagca
     4081 acatacttct ttataaatcc ctttaattga atatggtatt gcttatattc attttcagtt
     4141 aaatagtagc gataattata ttctattctc acagtaatca cctaccttcg ataaaaataa
     4201 ttcaacttgt cgataaccgt attcactcaa aatagattca ggatgatatt gcacaccaaa
     4261 aaccggaaat ctaatatgct caaatgccat aataatcgct tcatcgtttt ttgctgtaat
     4321 ctttaagcaa tttggaaaag tcgctccgtc agcaattaat gaatgataac gcattacatt
     4381 gaaattttga ggcagtcctt gaaaaatacc ttcattggta tggcgtaact gtgtagtatg
     4441 tccgtgtaca ggatgatagc cgtgaatgat atttccacca aaataagaca cgatacattg
     4501 aaatcctaaa catacaccta gtataggtac acgctgataa aattgttcta acacttcatt
     4561 caagatagga taatcatccg gattacccgg cccaggcgaa ataacaattg cttttggctt
     4621 catattaatg acgtcttcta tcagcagatt atcaatacca acaacttgaa ctgttagttt
     4681 cgtttgagtc ttaatatagt ctattaaatt atatgtaaat gaatcattat tatctatgac
     4741 tagaatcatt gtatactccg ttctaaatgt gttttatttt tataatatgt attggatgta
     4801 gctaaaactt taaaagcatt gtcattatcc tgaccttgat tttaactaat atatggtata
     4861 ttctattcat cgtacataaa tgaatatcag aggtttctag ctgaaaccct ctataaaaaa
     4921 ctagacattg aaatttcaaa cattcgttgg taaaccttta atata
//
LOCUS       DAOBBH010000172         4936 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.172, whole genome shotgun sequence.
ACCESSION   DAOBBH010000172 DAOBBH010000000
VERSION     DAOBBH010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.172"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            228..1130
                     /locus_tag="P6L01_002198"
     CDS             228..1130
                     /locus_tag="P6L01_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDP3031248.1"
                     /translation="MKFLSFKYNDKTSYGVKVKREDAVWDLTQVFADFAEGDFHPKTL
                     LAGLQQNHTLDFQEQVRKAVVAAEDSGKAEDYKISFNDIEFLPPVTPPNNVIAFGRNY
                     KDHANELNHEVEKLYVFTKAASSLTGDNATIPNHKDITDQLDYEGELGIVIGKSGEKI
                     PKALALDYVYGYTIINDITDRKAQSEQDQAFLSKSLTGGCPMGPYIVTKDELPLPENV
                     NIVTKVNNEIRQDGNTGEMILKIDELIEEISKYVALHPGDIIATGTPAGVGAGMQPPK
                     FLQPGDEVKVTIDNIGTLTTYIAK"
     gene            1459..1848
                     /locus_tag="P6L01_002199"
     CDS             1459..1848
                     /locus_tag="P6L01_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YisL family protein"
                     /protein_id="HDP3031249.1"
                     /translation="MLHLHILSWVLAIILFIATYLNISKNQGGSPFFKPLHMILRLFM
                     LLTLISGFWILIQSFMNGGANHMLLTLKMLCGVAVVGLMEVSIAKRKRHEQSHKMFWI
                     TMALIIITMVLGVILPLGPISKLFGIG"
     gene            complement(2020..3336)
                     /locus_tag="P6L01_002200"
     CDS             complement(2020..3336)
                     /locus_tag="P6L01_002200"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HDP3031250.1"
                     /translation="MPKIVVVGAVAGGATCASQIRRLDKESDIIIFEKDRDMSFANCA
                     LPYVIGEVVEDRKYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVTVLNRKTNEQF
                     EESYDKLILSPGASANSLGFESDITFTLRNLEDTDAIDQFIKANQVDKVLVIGAGYVS
                     LEVLENLYERGLHPTLIHRSDKINKLMDADMNQPILDELDKREIPYRLNEEIDAINGN
                     EITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKLDRKGFIPVNDKFETNVPNIYAIG
                     DIATSHYRHVDLPASVPLAWGAHRAASIVAEQIAGNDTIEFKGFLGNNIVKFFDYTFA
                     SVGVKPNELKQFDYKMVEVTQGAHANYYPGNSPLHLRVYYDTSNRQILRAAAVGKEGA
                     DKRIDVLSMAMMNQLTVDELTEFEVAYAPPYSHPKDLINMIGYKAK"
     gene            complement(3388..4212)
                     /locus_tag="P6L01_002201"
     CDS             complement(3388..4212)
                     /locus_tag="P6L01_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDP3031251.1"
                     /translation="MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATG
                     RPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQ
                     VSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIP
                     EIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIDRNNIIAFGD
                     EDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLNDFFNLNIRYYC"
     gene            4326..4634
                     /locus_tag="P6L01_002202"
     CDS             4326..4634
                     /locus_tag="P6L01_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDP3031252.1"
                     /translation="MEEALKDSILGALEMVIDPELGIDIVNLGLVYKVNVDDEGVCTV
                     DMTLTSMGCPMGPQIIDQVKTVLAEIPEIQDTEVNIVWSPPWTKDMMSRYAKIALGVS
                     "
BASE COUNT         1643 a          749 c          834 g         1710 t
ORIGIN      
        1 tacttaataa agaagttaaa ggttatttat acttcttcaa atttggtaca ttgcaactgt
       61 agtattttga ttttcaaaag aataaaaaat aatttcgatt aagtgcaaag tccttgtagc
      121 agaatgaaca caactcattt tcaaaattgt cttacttatt tatttgttat ttgataacga
      181 aaaaagttat aatgtgaatt aagataaaga tgaggagttg agaatgaatg aaattcttat
      241 cattcaagta taatgacaaa acttcatatg gcgttaaagt aaaacgcgaa gatgctgtat
      301 gggatttaac acaagtattt gctgactttg cagaaggaga tttccatcct aaaacattgt
      361 tagctggttt acaacaaaat catactttag attttcaaga acaagtacgt aaagcagttg
      421 tagcagcaga agatagcggc aaagctgaag actataaaat ttcatttaat gacattgaat
      481 tcttaccacc agtaacacct ccgaataatg tgattgcttt tggtagaaat tacaaagatc
      541 atgcgaacga attaaatcat gaagtagaaa aattatatgt atttacaaaa gcagcgtcat
      601 ctttaacagg agataatgca acaattccaa atcataaaga tattactgat caattagatt
      661 atgaaggtga attaggtatt gttattggta agtctggtga aaagattcca aaagcattag
      721 ctttagatta tgtttacggc tatacaatta ttaacgatat cactgatcgc aaagcacaaa
      781 gtgaacaaga tcaagcattt ttatcaaaaa gtttaactgg cggttgccca atgggtcctt
      841 atatcgttac taaagacgaa ctaccattac ctgaaaatgt aaatattgtt acaaaagtta
      901 acaatgaaat tagacaagat ggtaacactg gcgaaatgat tcttaaaatt gatgaattaa
      961 tagaagaaat ttcaaaatat gttgcactac atccgggaga tattattgca actggtacac
     1021 cagcaggcgt tggtgcaggt atgcaaccac ctaaattttt acaaccaggt gatgaagtta
     1081 aagtgactat tgataatatt ggaacgctga caacttatat tgctaaataa ttatcattta
     1141 aaaagctaac cagtctttat atagattggt tagttttttc ttgcttttct aaaaaggtgt
     1201 taaagataaa ttatttataa tgttaccatt ttgagatgaa agtgaaatat tgatattaag
     1261 aagtagttga ttattttaca gcagattcac aatattctaa taaggaataa aatgtcatgt
     1321 tcttcctctc aaatatagaa gtgtggtaga atatatattc gtgtataatc aaatcatgat
     1381 taaattacaa gcaagtgggt attaatccca agaagcttta atttaataat ataaataatt
     1441 aaagtggggg gaatcagtat gttacattta catatattaa gttgggtatt agcgattatt
     1501 ttatttatcg ctacatactt aaacatttca aaaaatcaag gcggatcacc atttttcaaa
     1561 ccgttgcaca tgattttacg cttatttatg ctgttgacgt taatttcagg attttggata
     1621 ttaattcagt catttatgaa tggcggggca aatcatatgt tgcttacatt gaaaatgctg
     1681 tgtggtgttg cagtagttgg attgatggaa gtgtcgattg ctaaaagaaa gagacatgaa
     1741 caaagtcaca aaatgttttg gataacaatg gcattaatta tcatcacaat ggtattaggt
     1801 gtcattctac cgttagggcc tatatcaaaa ttattcggta ttggctaatt gataagacat
     1861 atagtatatg acataaggca tggacaatta ttagtccaag accttatgtc ttttttgatt
     1921 ctaaaaatgt ttaaacataa aaaaaggact ggatagtagt accagtccaa cataattcta
     1981 acctagacaa ttaagattag attgaatcgc atgattcatt tatttagctt tgtaaccaat
     2041 catattgatt aaatctttag ggtggctata tggtggtgca taagccactt caaactcagt
     2101 taactcatct acagttagct ggttcatcat tgccatcgat agtacatcaa tacgtttatc
     2161 tgcaccttct tttcctactg cagctgctct taaaatctga cggtttgaag tgtcataata
     2221 tacccttaag tgtaaagggg aatttcctgg gtaataattc gcgtgtgcac cttgagtgac
     2281 ttccaccatt ttatagtcaa attgctttag ttcatttggt ttaacgccga cactcgcaaa
     2341 tgtataatca aagaacttca caatattgtt gcctaagaag cctttgaatt caatagtgtc
     2401 atttccagca atttgttcgg caacaatact tgctgcacgg tgagcgcccc aagctaaagg
     2461 aacactagcc ggtagatcga catgtcgata atgtgatgtt gcaatatcgc ctattgcata
     2521 aatgtttgga acatttgttt caaatttatc gtttaccggt atgaaacctt ttcgatcaag
     2581 tttgatattt gaactttcga taaattttga attggggtga gtaccgacac cttcaataat
     2641 catatcgtaa tgttcaactt ttcctgattt aaatgtaatt tcatttccat tgatagcatc
     2701 aatttcctca tttaaacggt atggaatctc ccgcttatct aattcatcaa gtataggttg
     2761 attcatgtca gcatccatta atttatttat cttatcagat cgatgaatta aagtagggtg
     2821 taaaccacgt tcgtaaagat tttcaagaac ttctaatgaa acataccctg cacctataac
     2881 caatacttta tcaacttgat ttgctttgat gaattgatcg atagcatcag tgtcttctaa
     2941 atttctaagt gtaaatgtaa tatcactttc aaagccaagg ctatttgcac ttgcaccagg
     3001 gcttaaaatg agtttatcgt aagattcttc aaattgttcg ttggtctttc tatttaatac
     3061 agttacagtt tgtctttcat cattgattgc aataacttca tgataagttt ttactgtaat
     3121 ttgctttcta tcataaaatt tttcaggtgt atacgctaaa gcatattttc tatcttcaac
     3181 aacttcgcca atgacataag gcaatgcaca attagcaaag ctcatatcac gatctttttc
     3241 aaaaataata atgtcacttt ctttatctaa acgtcgaatt tggctggcac atgttgcacc
     3301 gccagcgact gctccgacta cgactatttt gggcatcgta acttacctcc tattgtttta
     3361 aaatattata aaatgattag ttataaatta acagtaatat ctaatattta aattaaagaa
     3421 atcattcaaa tatcgaccaa tgccatcttc attattgttg aatgtaatat tgttcgctac
     3481 atctttaagt tcttgcaaac cattttccat agcaacacca tggcgggcgt actcaatcat
     3541 ttcaatatca ttatcttcat caccgaatgc aataatatta tttcggtcaa tatttaaaaa
     3601 ttgtctaact tgctcaatgc ctcttgcttt attaatacca agttttacaa tttcaatgac
     3661 agggaatggt gcgccccagc gtcgatgctc aatatgatcg gcataaaaat gagtaagcat
     3721 atttttgatt tcaggtattt tactttcttc ggcttcaatt aaaattgagg taggggattc
     3781 tttcaagtgg acaagtaaat taccagtttg aattcttgga ttacccattg aaaaaccttc
     3841 aaataatctt ggatcatgat tgttaatgaa aacataatct ttcacttctg ctataatatt
     3901 cgatacttga tattgttgta atccttgaat aatgttttgt gcgatgccta aatctaaaat
     3961 ttcatggcaa gttttgaagt ttttatcttt agggtgatgt acgtaagcgc cattaaaatt
     4021 aacaattggt gtcgttaaat ttaattcatg ataatacatt tgacttgcac gataaggtct
     4081 gccagtcgca atcataattt ggtgtccacg ttgttgtaat tcatttaata cttgtttagt
     4141 atatgatgaa atttctttgt tatcgtttaa taatgttccg tctaagtcta gacatattaa
     4201 atgtggttgc atatctctct cctcataagt tgcttagttc atatagatac atcatagcat
     4261 tttattggcg ttttgtcgga tatttgatat attatgaata atatgacatt aaagaggtga
     4321 tatggatgga agaggcattg aaagatagta tcttaggtgc attagaaatg gtaattgacc
     4381 ctgaattagg aattgatatc gttaatttag gtttagtata caaagtgaat gttgatgatg
     4441 aaggcgtatg tacagttgat atgactttaa catcaatggg atgtccaatg ggacctcaaa
     4501 ttattgatca agttaaaaca gtattagcag agattcctga aattcaggat actgaagtga
     4561 atatcgtatg gagtccacct tggacaaaag atatgatgtc acgttacgct aagattgcac
     4621 ttggtgtgag ctaacataaa cgtgcattgc ctgtagaatt tcttttagaa attctctgtg
     4681 ttgggtcccc gcagtcaata taacattgta gagtataatt cattgtgaat aagctccctt
     4741 caaagtagac attgaaaaat gaaaattttg aagggagcct tatttatgtt ctaggttctg
     4801 atttcatttt gaaaatttat atctaacttg tttcaaaaaa tatgaattaa gacagtgact
     4861 tgcacttcca agagtgcaca attcattttc tagttgaata ttttcttgaa aatttttata
     4921 ataaattgaa tttata
//
LOCUS       DAOBBH010000173         4893 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.173, whole genome shotgun sequence.
ACCESSION   DAOBBH010000173 DAOBBH010000000
VERSION     DAOBBH010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.173"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            32..1312
                     /locus_tag="P6L01_002203"
     CDS             32..1312
                     /locus_tag="P6L01_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDP3031253.1"
                     /translation="MKKLNIALLGLGTVGSGVVKIIEENRQQIQDTLNKDIVIKHILV
                     RDKSKKRPLNISQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVIT
                     ANKDLLAVHLKLLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNG
                     TSNFILSKMTKEQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVI
                     KLNDVKRRGISGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAA
                     VEDEYNAIYVIGDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFEL
                     KTDKTREMMDSDAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDN
                     QSYAAVIVGLESSPEELITKHGYEVDKVYPVEGV"
     gene            1318..2379
                     /locus_tag="P6L01_002204"
     CDS             1318..2379
                     /locus_tag="P6L01_002204"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDP3031254.1"
                     /translation="MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSKILG
                     IDLYVKYEGANPTGSFKDRGMVMAVTKAKEQGKKIVICASTGNTSASAAAYAARAGLK
                     AIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPFR
                     IEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQ
                     AEGASPIVQNKVIKNPETIATAIRIGNPASWDKATNALKESNGLIDSVTDDEILEAYQ
                     LMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNP
                     IKPLQNDKDSIIDYIKGAL"
     gene            2381..3295
                     /locus_tag="P6L01_002205"
     CDS             2381..3295
                     /locus_tag="P6L01_002205"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDP3031255.1"
                     /translation="MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWE
                     YIFHDDASKQLPTDETNFIYHVAQQVASKYSVDLPILCIEMRSDIPLARGLGSSASAL
                     VGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGFYNDVSKETSVAH
                     IDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAG
                     KLMQQDGFHEPYRQHLIAEFDEVKTIASQHNAYATVISGAGPTILIFSRKENSGELVR
                     SLNSQVVSCHSELVDINISGVKERIVYQ"
     gene            3353..4156
                     /locus_tag="P6L01_002206"
     CDS             3353..4156
                     /locus_tag="P6L01_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDP3031256.1"
                     /translation="MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQDEGYYVVLAS
                     GRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEVEVSKSIGKQDFDEIVDYCR
                     DRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLPMKRVDDIKAYIQGDVPKVMGVDYVA
                     NITEARIDLNGVFNDNVDATTSKPFFLEFMAKDVSKGNAIKALCHKLGYSVDQVIAFG
                     DSMNDKSMFEVAGLAIAMGNASDELKQYADEVTLDHNENGIPHALKKLL"
     gene            complement(4449..4763)
                     /locus_tag="P6L01_002207"
     CDS             complement(4449..4763)
                     /locus_tag="P6L01_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031257.1"
                     /translation="MIGQTNLFDDVLKQDERFVIVVQALEEKNGQLLKRTLREYPGLN
                     HKQMNDLFTHLKELFSEESFAENQSAFSITVYTNLDYTADQIYAHVKRFRGKHDWTQT
                     AK"
     gene            complement(4738..4842)
                     /locus_tag="P6L01_002208"
     CDS             complement(4738..4842)
                     /locus_tag="P6L01_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031258.1"
                     /translation="MLPFFILEILHIVYNIDNNNFSGVKFNDWTNKFI"
BASE COUNT         1771 a          663 c          951 g         1508 t
ORIGIN      
        1 tttctcaaaa gcacaaacgg aggtacataa aatgaaaaaa ttaaatatag cattattagg
       61 attaggtact gtcggatctg gtgttgttaa aatcatcgaa gagaaccgac agcaaattca
      121 agatacatta aataaagata ttgtcataaa gcatattctt gttcgagata aatctaaaaa
      181 gagaccgcta aatattagcc aatatcattt aactgaagat gttaatgaaa ttttaaatga
      241 tgattcatta gatattatcg ttgaagtcat gggaggaatt gaaccaactg tagattggtt
      301 aagaacagca cttaaaaata aaaaacatgt tattaccgca aataaagatt tattagcagt
      361 acatcttaaa cttttagaag atttagcaga agaaaatggt gtagctttaa agtttgaagc
      421 gagtgtagca ggtggtattc cgatcgtaaa tgccataaat aatggtttga atgcgaataa
      481 tatttcaaaa tttatgggaa ttttaaatgg tacctctaat tttattttat ctaaaatgac
      541 taaagagcaa acgacatttg aggaagcact tgatgaagcg aaaagacttg gttttgctga
      601 agcggatcca actgatgatg tagaaggggt agatgcagcg cgtaaagttg tcattacatc
      661 atatttatca tttaaccaag tcattaaatt aaacgacgtt aaacgaagag gaattagtgg
      721 cgtaacttta actgatatta atgtagccga tcaactgggg tataaaatta aattgattgg
      781 taagggaata tatgaaaatg gcaaagttaa tgcatcggta gaaccaacgt taattgataa
      841 aaagcatcaa ttagcagctg tagaggatga atataacgcg atttatgtta ttggtgatgc
      901 cgttggtgac acgatgtttt atggaaaagg agcaggcagt ttagcaacag gtagtgccgt
      961 tgtcagtgat ttattgaatg tagcattatt ctttgaatca gatttacaca cattgccacc
     1021 acattttgaa ttaaagacag ataaaacaag ggaaatgatg gattcagatg cagaaattaa
     1081 tattaaagaa aaatccaatt tctttgtagt agtgaatcat gtcaaaggtt caattgaaaa
     1141 ttttgaaaat gagttaaagg caatattacc atttcaccga tcattaagag ttgcaaatta
     1201 cgataatcaa tcatatgccg ctgttatagt tggattggaa tcatcaccgg aagaattaat
     1261 cactaagcat ggatacgaag ttgacaaagt atacccagta gaaggagttt aattataatg
     1321 agaagatggc aaggattagt agaagagttt aaagcacatt taccagtaaa tgaaaataca
     1381 ccaaaattaa cattgaacga gggaaataca ccactcattc attgtgaaaa tatgtctaaa
     1441 atactaggta tagatttata tgtgaagtat gaaggtgcca atccgacagg ttcatttaaa
     1501 gatcgcggta tggtaatggc tgtgacaaaa gcaaaagagc aaggtaagaa aattgtaata
     1561 tgcgcttcga ctggaaatac atcagcgtct gcagcagcat atgcagcgag agcaggttta
     1621 aaagctatcg tcgtaatacc agaaggtaaa attgcattag gtaaattgtc gcaagcagta
     1681 atgtatggtg cagaaatcgt ttctattgaa ggaaactttg atgaagcttt agaaattgta
     1741 aaagaaattg caaaaagtgg cgaaatagag cttgtaaact ctgtcaatcc atttagaatc
     1801 gaagggcaaa agacaggctc atttgaaatt gtacaacaat tagacggtga agcacctgat
     1861 attttagcga ttcctgtagg taatgcaggt aatattactg catattggaa aggctttaaa
     1921 gaatatcatg aagctaaagg atcacaattg ccgaaaatgt ttggcttcca agctgaaggc
     1981 gcatcaccaa ttgttcaaaa taaagtcatt aaaaatcctg aaacgattgc aactgctatt
     2041 cgaattggta atcctgctag ttgggataag gcgactaatg ctcttaaaga atcaaatgga
     2101 ttaatagata gtgttactga tgatgaaatt ctagaagcat atcagttaat gacaactaaa
     2161 gaaggtgtct ttagtgaacc agcaagtaat gcttctattg caggtttaat taaattgcat
     2221 agacaaggta aattacctca aggtaaaaaa gtagttgcta ttttaactgg taatggatta
     2281 aaagatcctg atactgctat ttcactacta gataatccga taaagccatt gcaaaatgat
     2341 aaagatagca ttatcgatta tattaaagga gctttataac atgtcgaatg ttttggagtt
     2401 aacaattcct gcatcaacag ccaaccttgg agttggcttt gattctatag gtatggcttt
     2461 agataaattt ttgcatctgt ctgtaaagga aacatcaggg acaaaatggg aatatatttt
     2521 ccatgatgat gcatctaagc aattgcctac tgacgaaaca aactttattt atcatgtagc
     2581 acaacaagtt gcttctaaat atagtgttga cttgcctatt ttatgtatcg aaatgagaag
     2641 tgatattcca ttggcaagag ggttaggttc gtcagcttct gctttagtag gagctatata
     2701 tatcgcaaat tattttggtg atatccaact gtctaaacat gaggtattac aattagcgac
     2761 tgaaatcgaa ggacatcctg ataatgttgc gccgaccatt tatggtggtt taatcgctgg
     2821 attttataat gatgtctcga aagaaacgtc agttgcacat atcgacatac cagacgtgga
     2881 tgtgattgta acgataccaa cttatgaact aaaaacagaa gcatcaagac gtgctttacc
     2941 acaaaaatta acacatagtg aagcggtaaa aagtagtgca attagtaata caatgatttg
     3001 tgcattagca cagcacaatt atgaattagc aggtaaactc atgcaacaag atggctttca
     3061 tgaaccgtat cgtcagcatt taattgctga atttgatgaa gtgaaaacaa ttgctagtca
     3121 acataatgcc tatgcaactg taattagtgg tgctggacca actattttaa tatttagtcg
     3181 taaagaaaat agtggggaat tggttcgctc tttaaatagt caggtagtat catgccattc
     3241 tgaattggtc gatattaata tcagtggtgt taaagaacga attgtatacc aatagacgct
     3301 ttatattgta aaatagtatt aaatgcagaa tagagaggag atttaatgcg atatgacaaa
     3361 ttataaagtt gtcgttttag atatggatga cacattgcta aattcagata atgtgatatc
     3421 agaagaaact gcaaattatt taacagcaat tcaagatgaa ggttattatg ttgttttagc
     3481 atctggtaga cctactgaag gtatgattcc aactgctaga gatttaaaat tacctgaaca
     3541 tcatagctat attattagtt ataacggtag taaaacgatt aacatgacta atgaagaagt
     3601 agaagtaagt aaatcgattg gtaagcaaga tttcgatgaa attgtagatt attgtcgaga
     3661 cagaggcttt ttcgttctta catatcatga tggtcaaatt atttacgaca gcgaacatga
     3721 gtatatgaat attgaagcag aattaacagg tttaccgatg aaacgtgttg atgatatcaa
     3781 agcgtatatt caaggcgatg tacccaaggt catgggtgta gattatgtag cgaatattac
     3841 agaagctaga attgatttga atggtgtgtt caatgataat gtagatgcta cgacaagtaa
     3901 gccattcttc ttagaattta tggccaaaga tgtttcaaaa ggtaatgcaa ttaaagcgtt
     3961 atgtcacaaa ttgggatatt cggtggatca agtcattgct tttggtgata gtatgaatga
     4021 taaatcaatg tttgaagtcg caggtctagc tattgctatg gggaatgcat cagatgaact
     4081 taagcaatat gcagatgaag ttacgttgga ccataatgaa aatggtattc cacatgcgct
     4141 caaaaaattg ttataaattt taaaataagc cttaacacat gatatttgaa taagatatct
     4201 tgtggttaag gctttttatt tatgtgaaaa tgacttcagt tatactatgg aggatttgaa
     4261 atacatattt tagattagta atgatatcaa acgaatagag taaatgtata ttttttgaat
     4321 aaatcaagta ttaactagtc acggaaggta ggtaaataaa tttgatcatt ctgaataata
     4381 acgaatgcat taaaaaatgc agtgacaatc taacattaat tgttaaaatc actgcaagca
     4441 tcttgatttt atttagctgt ttgtgtccag tcatgcttac ctctgaaacg ttttacatga
     4501 gcatatattt ggtcagcagt ataatctaaa tttgtataaa ctgtaatact gaacgctgat
     4561 tggttttcag caaatgattc ttcggaaaat aattccttta agtgcgtaaa taaatcattc
     4621 atttgtttat ggtttaaacc gggatattcc cttaaagttc tctttaataa ttgtccgttc
     4681 ttttcttcta atgcttgaac tacaataaca aatctttcat cttgctttag tacatcatca
     4741 aataaatttg tttgtccaat cattgaattt cacccctgaa aagttattgt tatctatatt
     4801 atacacgata tgcaaaattt ctaaaataaa gaatggtaac atggtaataa taaaatataa
     4861 acagaatact agttaataca ctttatacta gaa
//
LOCUS       DAOBBH010000174         4849 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.174, whole genome shotgun sequence.
ACCESSION   DAOBBH010000174 DAOBBH010000000
VERSION     DAOBBH010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.174"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(185..340)
                     /locus_tag="P6L01_002209"
     CDS             complement(185..340)
                     /locus_tag="P6L01_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031259.1"
                     /translation="MIIHCSIVSNFIFNLYIVTNFNLNFLSLKQIIYSKLIVRKFKIF
                     VDILKQI"
     gene            378..839
                     /gene="tsaE"
                     /locus_tag="P6L01_002210"
     CDS             378..839
                     /gene="tsaE"
                     /locus_tag="P6L01_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDP3031260.1"
                     /translation="MIKINNLDEMNQFAMFLVEQLKSGDLILLNGDLGAGKTTLTQFI
                     GKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIE
                     WSQFIKDLLPATHLSINISTISENTRQIELFAQGEHYEQIKEAIIHEFAAH"
     gene            820..1482
                     /gene="tsaB"
                     /locus_tag="P6L01_002211"
     CDS             820..1482
                     /gene="tsaB"
                     /locus_tag="P6L01_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDP3031261.1"
                     /translation="MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAIS
                     QLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKTLAYALDVKLYGVSSLKALAAT
                     IDHTDKLLVPVFDARRQAVYTGIFQWQNGQLVTILEDQYMSIEALRAFLEDLNQPFIY
                     IGKDTIKLQDELQGEVIAQLPNASVMYQLIDKPTDVHTFTPKYHKLAEAERNWINSQK
                     NN"
     gene            1455..1919
                     /gene="rimI"
                     /locus_tag="P6L01_002212"
     CDS             1455..1919
                     /gene="rimI"
                     /locus_tag="P6L01_002212"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDP3031262.1"
                     /translation="MDQQSKEQLNIREMTKEDVPQVFDLERRSFNDSSWTIDAFYHEI
                     EQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIDDNYRGYGLGQMLLKYGINYA
                     SHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND"
     gene            1912..2937
                     /gene="tsaD"
                     /locus_tag="P6L01_002213"
     CDS             1912..2937
                     /gene="tsaD"
                     /locus_tag="P6L01_002213"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDP3031263.1"
                     /translation="MTKDILILAVETSCDETSVSVIKNGRDILSNTVLSQIESHKRFG
                     GVVPEVASRHHVEGITTTINEALVDADVSMEDIDAIAVTEGPGLIGALLIGVNAAKAL
                     AFAYDKPLIPVHHIAGHIYANHIEEPLTFPLIALIVSGGHTELVYMKDHLSFEVIGET
                     RDDAVGEAYDKVARTIGLNYPGGPQVDRLAAEGEDTYSFPRVWLDKDSYDFSFSGLKS
                     AVINQLHNQRQKNIPIIEANVATSFQNSVVEVLTFKAIQACKEYSVQRLIVAGGVASN
                     KGLRQSLADQCKVNDIQLTIPSPKLCTDNAAMIGVAGHYLYQQGRFADLALNGHSNID
                     LEEYSAE"
     gene            complement(3252..4838)
                     /locus_tag="P6L01_002214"
     CDS             complement(3252..4838)
                     /locus_tag="P6L01_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002488504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HDP3031264.1"
                     /translation="MFVIAIILLTSVIGIVGRYMSRQRLLKSMETLWQTISPLEAFIR
                     PNSHFDYEYKLYKEKFESHSLVDDKTWSDLNMNAIFHKMNYNLTAIGEMKLYACLRGM
                     LSITNKSLLSLFNDNAEFRKNVTYHLALIGKTVYPTFPDQITPVKRQNILFLCPFLPV
                     ISFAVIFINSQVGILLFLMSCLFNIILSATLKRTYEDDLKSIFYASNVLKQGYTISKI
                     KHAPQPEVNFKQFRTARHLTSVLAEVNDEDIGAMVIKLVKLIFMLDYVLFHSIQKSYT
                     THMNELKNCFDYIAELDNHYALAMYRRTLECYTEPQIDDTNDGIVFSELTHPLIADAV
                     ANDFSLSQNILLTGSNASGKSTFMKSIAINIILASAIQTVTASKFVYQPGIVFTSMAN
                     ADDVLSGDSYFMAELKSIKRIVEIPDNQKIYCFIDEIFKGTNTTERIAASESVLSFLH
                     EKSNFRVIAATHDIELAELLKQRYENYHFNEVIENNNIHFDYKIKPGKANTRNAIELL
                     KITSFPAKIYERAKDNVSNG"
BASE COUNT         1666 a          683 c          891 g         1609 t
ORIGIN      
        1 acttaaagcg tttagtaaat atttactaac gttcgagtcc taaagagtat aataaaataa
       61 taatatttga agttgtcggt tgatgttcag tatattgatt aagcatttta ttatactcct
      121 tccgtgaatg gtatatatca ctttaatagt tagaattgtc taaaaatttg ataaatttaa
      181 tttactatat ctgctttagt atgtcaacaa atattttaaa ttttcttaca attaactttg
      241 aatagataat ctgtttcaaa gataagaaat ttaagttgaa atttgttaca atataaaggt
      301 taaatataaa gttagatacg attgaacaat ggataatcat ttttatgaat gaaaagcata
      361 atataggaga atcaacattg ataaagataa ataatttaga tgaaatgaat caatttgcta
      421 tgtttttagt tgagcaattg aaaagtggtg atttgatttt acttaacgga gatttaggag
      481 caggtaaaac aacgttaacg caatttatag gaaaagctct tggtgtaaga cgtacgatta
      541 attccccgac atttaacatc attaaatcat ataggggtaa aaatttaaaa ttgcatcata
      601 tggattgtta tcgcttagaa gattctgatg aagatttagg gtttgatgaa tttttcgaag
      661 atcaggcaat tactgttatt gaatggagtc aatttataaa agatttactt ccagcgacgc
      721 atttatctat taacatttca acaatatctg aaaatacaag acaaattgag ttgttcgcgc
      781 aaggagaaca ttatgaacaa attaaggagg caattatcca tgaattcgct gctcattgat
      841 acatcgaatc aaccattatc agtagcattg atgcaaaacg atgaagtttt agctgaaata
      901 actacggatt ctaaacaaaa tcattcagtg caattaatgc ctgcgattag tcagctattt
      961 gagcagagca aaatagctaa acagcaatta gatgcgatta ttgttgctga aggtcctggt
     1021 tcatatactg gcttacgtat cggtgttact gttgctaaaa cattagcgta tgcgttagat
     1081 gttaaattat atggtgtttc atcattaaag gcattggcag caacaatcga tcatactgat
     1141 aaattattgg ttcctgtgtt tgatgcaaga cgacaagcag tttatacagg catattccag
     1201 tggcaaaatg gtcaattagt gactatttta gaagatcaat atatgtcgat agaggcattg
     1261 cgagcttttt tagaagattt aaaccaacca tttatttata ttgggaaaga tactataaaa
     1321 ttacaagatg aattacaagg tgaagtgatt gcgcagctac caaatgcatc agtcatgtat
     1381 caattgatag ataaacctac agacgtacat acatttactc ctaaatacca taaattagct
     1441 gaggcggaac gaaattggat caacagtcaa aagaacaatt aaacattaga gagatgacta
     1501 aagaagatgt gccacaagtc tttgatcttg agcgtcggag tttcaatgac agttcttgga
     1561 caattgacgc attttatcat gaaatagagc aaaataactt tgcgaaatat tttgtcttag
     1621 aatttgaaca acaaattatt ggttatttag gtttatggat tgttatcgat caagctcaaa
     1681 ttacaacagt tgcaatcgat gataattata gaggctatgg tttaggtcaa atgcttttaa
     1741 aatacggaat aaactatgca agtcacactt gtgatgtgat gagtttagaa gtaagagtta
     1801 ataataaagt ggcacaacat gtatatgaaa atttaggatt tcaatatggt ggtaaacgta
     1861 aaaattatta tggtgaaggt gaggacgcaa tggttatgtg ggtgaattta aatgactaaa
     1921 gatatattaa tactagctgt tgaaacaagt tgtgatgaaa caagcgttag tgttataaaa
     1981 aatggcagag atattttatc aaatacagtt ttaagtcaga ttgaaagtca taaacgattt
     2041 ggcggtgtcg ttcccgaagt ggcaagtaga catcacgttg aaggtataac aacaacaata
     2101 aacgaggctc tagtggatgc cgatgtatca atggaagata ttgatgccat agcggttaca
     2161 gaaggccctg gactaattgg tgcgttacta ataggtgtta atgcagccaa agcattggca
     2221 tttgcttacg ataagccact tattcctgtt catcatattg caggacatat atatgctaat
     2281 cacatagaag agccattaac attcccgcta attgcactta ttgtttcagg tggacatact
     2341 gaattagttt atatgaaaga tcatttatca tttgaagtca ttggtgaaac acgagatgac
     2401 gcagtaggtg aggcttatga taaagtggca cgaacaattg gtttaaatta tccaggtggt
     2461 ccacaagttg atcggttggc tgctgaaggt gaagatactt attcattccc tcgtgtttgg
     2521 ttggataaag atagttatga ttttagtttt agtgggttga aaagtgccgt gatcaatcaa
     2581 cttcacaatc aacgacaaaa aaatattcca atcattgaag ctaacgtagc aacgagcttt
     2641 caaaatagtg ttgtagaggt gcttacgttt aaagctattc aagcttgtaa agaatatagt
     2701 gttcagcgat taattgttgc tggtggcgtg gcgagtaata aaggattacg tcaatcttta
     2761 gcggatcaat gcaaagtcaa tgacattcaa ttaactatcc caagtcctaa attatgcaca
     2821 gataatgctg caatgatagg cgttgccggc cactatttgt atcagcaagg tcgatttgct
     2881 gatttagcat taaatgggca cagcaatata gatttagaag agtattctgc agaataaaat
     2941 aaacgtcagt tattaacatg attacgatga acattattga aactgttcat agtcttctgt
     3001 catgtgatag ctgacgtttt tatattgcaa ttaggggagt gagacagaaa agataaagaa
     3061 ccactaatga tttattatgt tgggattctg ccaacttgca cattattgta agctgacttt
     3121 ccgccagcct ctgtattggg gcccccggac tagtattgaa aaaacttatt acaagcgtat
     3181 tttcatttag tcaaagactg tcaatataac aatgttgcga cttgaacatt gattgatgtt
     3241 caagattcat gctaaccatt ggaaacatta tcttttgctc gttcatatat ttttgctgga
     3301 aatgaagtga tttttaataa ttcgatggca ttacgagtat ttgctttgcc aggcttaatt
     3361 ttgtaatcaa aatgtatgtt attattttct attacctcat tgaaatgata attttcataa
     3421 cgttgtttta agagttcagc taactcaata tcatgtgttg ctgcaataac tctaaagtta
     3481 gatttttcat gtaaaaatga tagtactgat tctgaagcgg caattcgttc agttgtgttg
     3541 gtacctttaa aaatttcatc gataaagcag tatatttttt gattatctgg aatttcaaca
     3601 attcgcttaa tagattttaa ttcagccata aaataactat cgcctgataa cacatcatcg
     3661 gcatttgcca ttgacgtgaa cacaattcca ggttgatata caaatttgct tgcggtaact
     3721 gtttgtatag ccgatgcaag aataatgttt attgctatgg atttcataaa tgttgattta
     3781 cccgaagcat tagacccagt taacagtata ttttgtgaaa gtgagaaatc attggctact
     3841 gcatctgcta tgagtggatg tgttaactca gagaatacta tgccatcatt tgtatcatca
     3901 atttgtggtt cagtataaca ttctagcgtt ctacgataca ttgctaatgc ataatgatta
     3961 tctaattcag ctatgtaatc gaaacaattt ttaagctcat tcatatgtgt tgtatagctt
     4021 ttttgaattg aatgaaataa aacataatca agcatgaaaa taagtttaac tagtttaatt
     4081 accatagctc cgatatcttc atcattaact tcagctaaaa cactcgttaa atgacgtgct
     4141 gttctaaatt gcttaaaatt gacttcaggt tgtggagcat gtttaatttt tgagatagta
     4201 tagccttgtt ttaaaacatt ggatgcataa aagatagatt ttaagtcatc ctcatatgta
     4261 cgctttaaag ttgctgataa aatgatatta aataggcaac tcataagaaa taataaaata
     4321 ccaacttgtg aattaataaa tataactgca aatgagataa ctggtaaaaa cgggcataga
     4381 aacaatatat tttgacgttt taccggtgtg atttggtctg gaaatgttgg ataaacagtt
     4441 ttaccaatca aagctaaatg atatgttacg ttttttctaa attcagcatt atcattaaat
     4501 aaactaagta atgatttgtt cgtaattgaa agcattccac gtaaacaggc atatagcttc
     4561 atttcaccaa tagctgttaa attataattc atcttatgaa aaattgcatt catatttaag
     4621 tcggaccaag ttttatcatc aactaatgaa tgtgattcaa atttttcctt gtagagctta
     4681 tactcatagt cgaaatgtga gttcggtctg ataaaagctt ctaatggaga aatcgtttgc
     4741 cataatgttt ccatagattt taatagacgt tgacgactca tgtatcgtcc aacaattcct
     4801 attacagatg taagtagaat aattgctata acaaatatta aaaatgttt
//
LOCUS       DAOBBH010000175         4798 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.175, whole genome shotgun sequence.
ACCESSION   DAOBBH010000175 DAOBBH010000000
VERSION     DAOBBH010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.175"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            318..1442
                     /locus_tag="P6L01_002215"
     CDS             318..1442
                     /locus_tag="P6L01_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDP3031265.1"
                     /translation="MTVQSAYIHIPFCVRICTYCDFNKYFIQNQPVDEYLDALITEMS
                     TAKYRNLKTMYVGGGTPTALSINQLERLLKAIRDTFTITGEYTFEANPDELTKEKVQL
                     LEKYGVNRISMGVQTFKPELLSVLGRTHNTEDIYTSVLNAKNAGIKSISLDLMYHLPK
                     QTIEDFEQSLDLALDMDIQHISSYGLILEPKTQFYNMYRKGLLKLPNEDLGADMYQLL
                     MSKIEQSPFHQYEISNFALDGHESEHNKVYWFNEEYYGFGAGASGYVDGVRYTNINPV
                     NHYIKAINKESKAILVSNKPSLTERMEEEMFLGLRLNEGVSSSRFKKKFDQSIESVFG
                     QTINNLKEKELIVEKNDAIALTKRGKVIGNEVFEAFLIND"
     gene            1542..2519
                     /gene="hrcA"
                     /locus_tag="P6L01_002216"
     CDS             1542..2519
                     /gene="hrcA"
                     /locus_tag="P6L01_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HDP3031266.1"
                     /translation="MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRN
                     EMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQY
                     DVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVE
                     HVHLASDIPFSNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINKLINTMN
                     NHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKI
                     GNEIDDSLSDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW"
     gene            2551..3177
                     /gene="grpE"
                     /locus_tag="P6L01_002217"
     CDS             2551..3177
                     /gene="grpE"
                     /locus_tag="P6L01_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDP3031267.1"
                     /translation="MTNKDESVEKNTESTVEETNIKQNIDDSVEQAEESKGHLQDEAI
                     EETSDENVIEEIDPKDQKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQ
                     AQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
                     EAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ"
     gene            3246..>4798
                     /gene="dnaK"
                     /locus_tag="P6L01_002218"
     CDS             3246..>4798
                     /gene="dnaK"
                     /locus_tag="P6L01_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDP3031268.1"
                     /translation="MSKIIGIDLGTTNSCVTVLEGDEPKVIQNPEGSRTTPSVVAFKN
                     GETQVGEVAKRQAITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAE
                     SYLGEKVDKAVITVPAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDK
                     DEKVLVFDLGGGTFDVSILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKEN
                     GVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFE
                     ELSDSLIRRTMEPTRQAMKDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEIGKEPNKG
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSK
                     SQIYSTAVDNQPSVDVHVLQGERPMAADNKTLGRFQLTDIPPAERGKPQIEVTFDIDK
                     NGIVNVTAKDLGTNKEQRITIQSSSSLSDEEIDRMVKDAEVNAEADKKRREEVDLRNE
                     ADSLVFQVE"
BASE COUNT         1847 a          689 c          865 g         1397 t
ORIGIN      
        1 gttggggtgg gacaacgaaa taaattttgc gaaaatatca tttctgtccc actcccttat
       61 gcatgagttt tactcatgta attttatttt taaggacata ttacatctgg ctaatgtgta
      121 agagccacta cataataaat cattagtggt tctttattat ttctatctca ctccctctaa
      181 acaagaataa atatcaaaat gaatcgatat attagacaac cattgattaa acgttaaagt
      241 taaaagtaag aataacagca gatagtccaa caggatgtag ccgattggat aaacagtctg
      301 agaagcgggg cattaaaatg acggtacaaa gtgcatatat acatattcca ttttgtgtaa
      361 gaatatgtac atattgtgat ttcaataaat attttataca gaatcaacct gtagatgagt
      421 acttagatgc actaataaca gaaatgtcta cagcaaaata taggaactta aagaccatgt
      481 atgtaggtgg cggcacacca acggcccttt ctattaatca gttggaaaga ttacttaaag
      541 caatacgtga tacgtttaca atcacaggcg agtatacatt tgaagcaaat cctgatgagt
      601 taactaaaga gaaagtccaa ctattagaga aatatggagt aaatagaatt tcaatgggcg
      661 ttcaaacatt caaaccggag ttattgtctg ttttaggtag aacgcacaat actgaagata
      721 tttacacttc ggtgttaaat gctaaaaacg caggtattaa atcaatcagt ttagatttaa
      781 tgtatcattt accgaaacag acgattgaag attttgaaca aagtttagat ctagctttag
      841 atatggatat tcaacatatt tcgagttacg gcttaatact tgaacctaaa acccaatttt
      901 ataatatgta tagaaaaggg ttgcttaaac ttcctaatga ggatttaggt gctgacatgt
      961 atcagttgtt gatgtctaag atagaacaat ctcctttcca tcaatacgaa atatctaatt
     1021 ttgcattaga tggccatgaa tcagaacata ataaggttta ctggtttaat gaggaatatt
     1081 atggatttgg agcaggtgca agtggttatg tagatggtgt gcgttatacg aatatcaatc
     1141 cagtgaatca ttatatcaaa gctataaata aagaaagtaa agcaatttta gtatcaaata
     1201 aaccttcttt gactgagaga atggaagaag aaatgtttct tgggttgcgt ttaaatgaag
     1261 gtgtgagtag tagtaggttc aaaaagaagt ttgaccaatc tattgaaagt gtctttggtc
     1321 aaacaataaa taatttaaaa gagaaagaat taattgtaga aaagaacgat gcgattgcac
     1381 taacaaagcg agggaaagtc ataggtaatg aggtttttga agctttccta ataaatgatt
     1441 aaaaaaattg aaatttcgag tctttaacat tgacttactt tgaccaattt gataaattat
     1501 aattagcact tgagataagt gagtgctaat gaggtgaaaa catgattaca gataggcaat
     1561 tgagtatatt aaacgcaatt gttgaggatt atgttgattt tggacaaccc gttggttcta
     1621 aaacactaat tgagcgacat aacttgaatg ttagtcctgc tacaattaga aatgagatga
     1681 aacagcttga agatttaaac tatatcgaaa agacacatag ttcttcaggg cgttcgccat
     1741 cacaattagg ttttaggtat tatgtcaatc gtttacttga acaaacatct catcaaaaaa
     1801 caaataaatt aagacgatta aatcaattgt tagttgagaa tcaatatgat gtatcatcag
     1861 cattgacata ttttgcagat gaattatcaa atatatctca atatacaact ttagttgttc
     1921 atcctaatca taaacaagat attatcaata atgtacattt gattcgtgct aatcctaatt
     1981 tagttataat ggttatcgta ttttcatcag gtcatgttga gcatgtacat cttgcttcag
     2041 acataccgtt tagtaatgat aaactaaata caatatcaaa ttttgttact aacaaactaa
     2101 ctgagtttaa tcagaattta caagatgata ttgtctcttt tgttcaatca gagcaggaag
     2161 aaatatttat caataaattg ataaatacga tgaataatca tatttctaat caaagtaata
     2221 gcatttatat gggtggaaag gttaaactta ttgatgcatt gaatgaaagt aatgtatctt
     2281 caattcagcc cattctacaa tatatagaat caaatagaat tgcagaatta ttacaagata
     2341 tatcttcacc aaatattaat gtaaaaattg gtaatgaaat tgatgacagc ttaagtgata
     2401 tttcaattgt aacaagtcaa tatcattttg atgaaacgtt aaaaggtcaa atcgctgtga
     2461 ttggacctac agctatgcat tatcaaaatg tcattcaatt attaaataga atttggtaat
     2521 gatggattag aaagtattgg aggacagaaa atgacaaata aagacgaatc agttgaaaaa
     2581 aacactgaat caacagttga agaaacaaac atcaaacaaa atattgatga ttcagttgaa
     2641 caagctgaag aaagtaaagg tcatttacaa gatgaagcaa tagaagaaac gtctgacgag
     2701 aatgttattg aagaaataga tccaaaagat caaaaaatta atgaacttca acaattagca
     2761 gatgaaaacg aagagaaata tttaaggctc tacgctgagt ttgaaaatta taagcgtaga
     2821 attcaaaaag aaaatgaaat aaataaaaca tatcaagcac aacgtgtgtt aacagatatt
     2881 ttaccagcaa tagacaatat agaacgtgca cttcaaattg aaggtgatga tgagactttt
     2941 aaatctcttc aaaaaggtgt acaaatggtg catgaaagtt tgattaacgc actaaaagat
     3001 aatggtcttg aagttattaa aactgaaggt gaagcatttg atccaaatat tcaccaagct
     3061 gtagttcaag atgataaccc tgattttgaa tctggcgaaa tcactcaaga actacaaaaa
     3121 ggatacaagc ttaaagatag agtattaaga ccatcaatgg tcaaagtaaa ccaataactt
     3181 aaatttggcg aaaagacatt gtttaaaatt aaattaattt aatgattaaa tggaggaatt
     3241 ttattatgag taaaattatt ggtatagact taggtacaac aaattcatgt gtaacagtat
     3301 tagaaggcga tgagccaaaa gtaattcaaa accctgaagg ttcacgtaca acaccatctg
     3361 ttgtagcttt caaaaatgga gaaactcaag ttggtgaagt agctaaacgt caagctatta
     3421 caaacccaaa cactgttcaa tctattaaac gtcatatggg tactgattat aaagtagata
     3481 ttgaaggtaa atcatacaca ccacaagaaa tctcagctat gattttacaa aacttaaaaa
     3541 atacagctga aagctattta ggtgagaaag ttgacaaagc tgtaattaca gtacctgcat
     3601 actttaacga cgctgaacgt caagcaacta aagatgctgg taaaattgct ggtttagaag
     3661 ttgagcgtat cattaatgaa ccaacagctg cagcattagc atatggttta gataaaactg
     3721 ataaagatga aaaagttctt gtttttgact taggtggcgg tacatttgac gtatctatcc
     3781 tagaattagg tgacggtgta ttcgaagtac tatcaacagc cggtgacaac aaacttggcg
     3841 gtgatgattt tgaccaagta attattgact acctagttgc agaattcaaa aaagaaaatg
     3901 gcgtagactt atctcaagat aaaatggcat tacaacgttt gaaagatgct gctgaaaaag
     3961 ctaaaaaaga cttatcaggt gtatcacaaa ctcaaatctc attaccattt atctcagctg
     4021 gtgaaaacgg tccattacac ttagaagtaa acttaactcg ttctaaattt gaagaattat
     4081 cagattcatt aattagaaga acaatggaac ctacacgcca agcaatgaaa gacgctggct
     4141 taacaaactc agatatcgat gaagttatct tagttggtgg atcaactcgt attccagcag
     4201 tacaagaagc tgtcaaaaaa gaaatcggta aagaacctaa caaaggtgta aacccggacg
     4261 aagtagtggc aatgggagct gcaatccaag gtggcgttat cacaggtgac gttaaagacg
     4321 tagtattatt agacgtaaca ccactatctt taggtattga aattttaggt ggacgtatga
     4381 atacgttaat tgaacgtaac actacgattc ctacatctaa atctcaaatc tattcaacag
     4441 cagtagataa tcaaccatca gtagatgtac acgtattaca aggtgaacgt ccaatggctg
     4501 cggataataa aacacttggt agattccaat tgactgatat tccaccagct gaacgtggta
     4561 aacctcaaat tgaagtaacg tttgatatcg ataaaaacgg tattgtaaat gtaactgcaa
     4621 aagacttagg tacaaataaa gaacaaagaa ttacaattca atcaagttct tcattatcag
     4681 acgaagaaat cgaccgtatg gtaaaagatg ctgaagttaa cgctgaagca gataaaaaac
     4741 gtcgtgaaga agtagactta agaaacgaag ctgacagtct agtattccaa gttgaaaa
//
LOCUS       DAOBBH010000176         4714 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.176, whole genome shotgun sequence.
ACCESSION   DAOBBH010000176 DAOBBH010000000
VERSION     DAOBBH010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.176"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(1..1356)
                     /locus_tag="P6L01_002219"
     CDS             complement(1..1356)
                     /locus_tag="P6L01_002219"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDP3031269.1"
                     /translation="MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPR
                     VLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISAS
                     HNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQK
                     YLSYLKSTVDVNFEGLKIVLDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKC
                     GSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQE
                     LNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVM
                     MDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDV
                     KEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLD
                     K"
     gene            complement(1383..2315)
                     /locus_tag="P6L01_002220"
     CDS             complement(1383..2315)
                     /locus_tag="P6L01_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HDP3031270.1"
                     /translation="MLESKWGLRFIAFLLALFFFLSVNNVFGNIFNTGNLGQKSSKTI
                     QDVPVEILYNTKDLHLTKAPETVNVTISGPQSKIIKIENPEDLRVVIDLSNAKAGKYQ
                     EKYQVKGLADDIHYSVKPKLANITLENKVTKKMTVQPDVSQSDIDPLYKITKQEVSPQ
                     TVKVTGGEEQLNDIAYLKATFKTNKKINGDTKDVAEVTAFDKKLNKLNVSIQPNEVNL
                     QVKVEPFSKKVKVNVKQKGSLADDKELSSIDLEDKEIEIFGSRDDLQNISEVDAEVDL
                     DGISESTEKTVKINLPEHVTKAQPSETKAYINVK"
     gene            complement(2317..3126)
                     /locus_tag="P6L01_002221"
     CDS             complement(2317..3126)
                     /locus_tag="P6L01_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDP3031271.1"
                     /translation="MDFSNFFQNLSTLKIVTSILDLLIVWYVLYLLITVFKGTKAIQL
                     LKGILVIVIGQQISMILNLTATSKLFDIVIQWGVLALIVIFQPEIRRALEQLGRGSFL
                     KRYTSNTYSKDEEKLIQSVSKAVQYMAKRRIGALIVFEKETGLQDYIETGIAMDSNIS
                     QELLINVFIPNTPLHDGAMIIQGTKIAAAASYLPLSDSPKISKSLGTRHRAAVGISEV
                     SDAFTVIVSEETGDISVTFDGKLRRDISNEIFEELLAEHWFGTRFQKKGVK"
     gene            complement(3315..4223)
                     /gene="rocF"
                     /locus_tag="P6L01_002222"
     CDS             complement(3315..4223)
                     /gene="rocF"
                     /locus_tag="P6L01_002222"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HDP3031272.1"
                     /translation="MTKTKAIDIIGAPSTFGQRKLGVDLGPTAIRYAGLISRLKQLDL
                     DVYDKGDIKVPAVNIEKFHSEQKGLRNYDEIIDVNQKLNKEVSASIENNRFPLVLGGD
                     HSIAVGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLE
                     LNSNVIKPENIVLIGMRDLDKGERQFIKDHNIKTFTMSDIDKLGIKEVIENTIEYLKS
                     RNVDGVHLSLDVDALDPLETPGTGTRVLGGLSYRESHFALELLHQSHLISSMDLVEVN
                     PLIDSNNHTAEQAVSLVGTFFGETLL"
     gene            complement(4361..4433)
                     /locus_tag="P6L01_002223"
     tRNA            complement(4361..4433)
                     /locus_tag="P6L01_002223"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4398..4400),aa:Lys,seq:ttt)
     gene            complement(4437..4511)
                     /locus_tag="P6L01_002224"
     tRNA            complement(4437..4511)
                     /locus_tag="P6L01_002224"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4477..4479),aa:Gln,seq:ttg)
     gene            complement(4521..4604)
                     /locus_tag="P6L01_002225"
     tRNA            complement(4521..4604)
                     /locus_tag="P6L01_002225"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4568..4570),aa:Tyr,seq:gta)
     gene            complement(4634..4709)
                     /locus_tag="P6L01_002226"
     tRNA            complement(4634..4709)
                     /locus_tag="P6L01_002226"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4674..4676),aa:Val,seq:tac)
BASE COUNT         1451 a          950 c          630 g         1683 t
ORIGIN      
        1 ttatttatct aatcccattt tatcttgaac cacatcagct atttgttgtg caaatctttc
       61 agcatcttca tcagttgctg cttcaaccat gacacgaact aatggttctg ttccagaagg
      121 tcttactaaa attcgacctt ctccattcat ttctacttct actttagtca taacttcttt
      181 aacgtcaaca ttttcttcaa cacgatattt atctgttacg cgtacgttaa ttaatgattg
      241 tggatatttt ttcatttgtc cagctaattc acttagtgat ttaccagtca tttttattac
      301 agaagctaat tgaataccag ttaataaacc atcaccagtt gtattgtaat ccatcataac
      361 gatatgccca gattgttctc cacctaagtt ataattaccg cgacgcattt cttctactac
      421 atatctgtcg ccaactttag ttttattaga tttaattcct tcttgttcaa gcgctttgta
      481 aaaacctaaa ttactcataa cagtagaaac aatcatgtca ttattcaatt cttgattttt
      541 atgcatttct tgaccaataa taaacataat ttggtcaccg tcaacgattt gaccattctc
      601 atctactgct atgattctgt ctccatcgcc gtcaaatgct aacccaaaat cactttcagt
      661 ttcaactact ttttcagcta atttttcagg atgtgtagag ccacatttct cattgatatt
      721 atatccatca ggactacatc caattgtttc agtatctgct tctaaatcac caaataagaa
      781 tggcgctagt gatgatgttg aaccatttgc accatctaaa acaattttca aaccttcaaa
      841 gttaacatct actgttgatt ttaaatagct caaatatttt tgtgcccctt caaagtaatc
      901 tgaataatgt acaatatcat tgccaactgg tcttggtaat tctggatttt cttgatccaa
      961 taatgcttca atttcatttt cttgttcatc tgatagttta aaaccatctg atccaaagaa
     1021 tttaatacca ttatctgcaa ctggattatg agaggctgaa atcattacac ctaactctgc
     1081 acccatatcg cgtgttaaat atgcaacacc tggtgttgaa ataataccta atcgcatcac
     1141 ttctgcacca attgaaatca aaccagctat taatgctgat tctaacattt cacctgaaac
     1201 tctagtatcg cgacctacaa gtacacgtgg gtgtttttca cctttattat gtgctagaac
     1261 atagccaccg tatcttccta atttaaatgc caattcaggt gttagttctt ggtttgcgac
     1321 acctcttact ccgtctgtac caaaatattt tcccattgtt tactctcctt taatttagct
     1381 atttatttta catttatata agccttcgtt tcacttggtt gtgctttagt gacatgttct
     1441 ggtaaattga tttttacagt cttttcagtt gattctgaaa taccatctaa atctacttct
     1501 gcatcaactt cgcttatatt ttgtaagtca tctcgactac cgaagatttc aatttcttta
     1561 tcttctaaat caatcgaact taactcttta tcatctgcta aactaccttt ctgtttaaca
     1621 tttactttaa cctttttgct aaaaggctct actttaactt gtaaattcac ttcattaggt
     1681 tgaatcgata catttaattt attcagtttt ttatcaaaag ccgttacttc tgcgacatct
     1741 tttgtgtcac cattaatctt tttattagtt ttaaaagtgg cttttaaata agcgatatca
     1801 ttcaattgtt cttctccacc tgttacttta actgtttgtg gtgaaacttc ttgctttgta
     1861 attttataaa gtggatcaat atcactctga cttacatcag gttgaactgt catcttttta
     1921 gttactttgt tttcaagcgt aatatttgct aatttaggtt ttacagaata atgaatgtca
     1981 tcagctaacc ctttaacttg atacttctct tgatattttc cagctttagc atttgataaa
     2041 tcaatcacta ctcttaaatc ttctggattt tcaattttta ttatctttga ttgtggtcct
     2101 gaaatagtca cattaactgt ttcaggcgct tttgttaaat gcaaatcttt agtgttataa
     2161 agaatttcaa cgggtacatc ttgaatcgtt ttactagact tttgaccaag attaccagtg
     2221 ttaaagatat ttccaaaaac attgttaaca gataaaaaga aaaacaatgc caaaagaaag
     2281 gcaataaatc tcaagcccca tttactttct agcatattat ttcacacctt tcttttgaaa
     2341 gcgtgtgcca aaccaatgtt cagcaagcaa ctcttcaaaa atttcgtttg aaatgtctcg
     2401 tcgtaatttt ccatcaaatg ttaccgaaat atcaccagtt tcttcagata caataacggt
     2461 aaatgcatca gatacttctg aaataccaac cgcagctcta tgtcttgtac ccaaactttt
     2521 agatatctta ggactatcag acaatggcaa ataacttgct gctgctgcaa tcttagtgcc
     2581 ttgaataatc attgcaccat catgtaaagg tgtgttaggt ataaagacat taattaaaag
     2641 ttcttgcgaa atatttgaat ccattgcaat acctgtttca atataatctt gaagacctgt
     2701 ttctttttca aagacaatta atgcacctat acgtctttta gccatatatt gcacagcctt
     2761 tgaaaccgat tgaatcaatt tctcttcatc tttactatac gtattagaag tatagcgttt
     2821 taaaaagcta cctctaccaa gttgttctaa cgcacgtcta atttctggtt ggaatattac
     2881 tattaaagct aatacccccc attgaataac gatatcgaat aatttagatg ttgcagtcaa
     2941 gttcaatatc atacttatct gctgaccaat aacaattact aatatccctt taagtaattg
     3001 tatcgcttta gttcccttaa agaccgtgat gagaagataa agtacatacc aaactatcag
     3061 taaatcaagg atactcgtta caatttttaa cgtactgagg ttttgaaaaa agttggaaaa
     3121 atccataaca tctcctccgg gtaatatttt tccataatac ccattatacc aatcatttac
     3181 atcactgtca tacgaacata tatttaaata gaaaaaattt attttaaaga ttataactac
     3241 tcttaatcat tttagtgaat taaaaaaagt agtgcaaaaa gcaaaatata ctttatacac
     3301 tacaaatcat ttatttataa taaagtttca ccaaaaaatg ttccaactaa tgaaaccgct
     3361 tgttcagcag tatgattatt actgtcaatc aatggattta cttcaactaa atccattgag
     3421 gaaattaaat gtgattgatg cagtaattcc aatgcaaaat ggctttctct ataactaaga
     3481 ccacccaaaa ctctagtacc agtgcctggc gtttcaagcg gatctaaagc atcaacatct
     3541 aaagataaat gaacgccatc aacattgcgt gacttcaaat attctattgt attttcaatt
     3601 acttccttta tccctaattt atcaatatct gacatagtaa atgttttaat attatgatct
     3661 ttgataaatt gtctttcacc tttatctaaa tctctcatac caattagtac gatgttttct
     3721 ggcttgatta cattactatt taattctaaa agttctttgg gaccttcgcc tgtcaaaatc
     3781 cttagaggca taccatgaat atttccactt ggtgactctt caggtatatt taaatcacca
     3841 tgtgcatcat accaaataac acctaaatta ttataatgtt tacttattgc tgatactgaa
     3901 cctaccgcaa tagaatgatc accaccaaga actagaggaa atctgttatt ttcaattgat
     3961 gctgaaacct ctttatttaa tttttgatta acatctataa tttcatcata atttcttaat
     4021 cctttttgtt cactatgaaa tttttcaatg ttcacagcag gtaccttaat atcccccttg
     4081 tcatatacat caaggtctaa ttgctttaat cttgaaatta atccagcata tctaattgct
     4141 gttggtccta aatcaacacc taattttctt tgtccaaatg ttgatggtgc acctataata
     4201 tcaattgctt ttgtctttgt cataagcgtc cccctttgct ctatgtaatt aaagaataat
     4261 gtatgcgctt accattatca agcaatagct acacatataa tctgtttatc ttattacttc
     4321 ataaaaaaaa gcacttccca aaaatggaaa gtgcaagtag tgagccatag aggattcgaa
     4381 cctctgaccc tctgattaaa agtcagatgc tctaccaact gagctaatgg ctctaatggc
     4441 tgggctagct ggattcgaac caacgagtga cggagtcaaa gtccgttgcc ttaccgcttg
     4501 gctatagccc aatatataga tggtggaggg gggcagattc gaactgccga acccgaagga
     4561 gcggatttac agtccgccgc gtttagccac ttcgctaccc ctccagctta ttcatataat
     4621 ttaataatca aaatggtgga gaatgacggg ttcgaaccgc cgaccctctg cttgtaaggc
     4681 agatgctctc ccagctgagc taattctcca aaat
//
LOCUS       DAOBBH010000177         4681 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.177, whole genome shotgun sequence.
ACCESSION   DAOBBH010000177 DAOBBH010000000
VERSION     DAOBBH010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.177"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..645
                     /gene="sarS"
                     /locus_tag="P6L01_002227"
     CDS             <1..645
                     /gene="sarS"
                     /locus_tag="P6L01_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarS"
                     /protein_id="HDP3031273.1"
                     /translation="FILLTYLFHQQENTLPFKKIVSDLCYKQSDLVQHIKVLVKHSYI
                     SKVRSKIDERNTYISISEEQREKIAERVTLFDQIIKQFNLADQSESQMIPKDSKEFLN
                     LMMYTMYFKNIIKKHLTLSFVEFTILAIITSQNKNIVLLKDLIETIHHKYPQTVRALN
                     NLKKQGYLIKERSTEDERKILIHMDDAQQDHAEQLLAQVNQLLADKDHLHLVFE"
     gene            1066..2568
                     /gene="spa"
                     /locus_tag="P6L01_002228"
     CDS             1066..2568
                     /gene="spa"
                     /locus_tag="P6L01_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal protein A"
                     /protein_id="HDP3031274.1"
                     /translation="MKKKNIYSIRKLGVGIASVTLGTLLISGGVTPAANAAQHDEAQQ
                     NAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNNFNK
                     DQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNK
                     EQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNK
                     EQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNK
                     EQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDNKKP
                     GKEDGNKPGKEDGNKPGKEDNKKPGKEDGNKPGKEDNNKPGKEDGNKPGKEDNNKPGK
                     EDGNKPGKEDGNGVHVVKPGDTVNDIAKANGTTADKIAADNKLADKNMIKPGQELVVD
                     KKQPANHADANKAQALPETGEENPFIGTTVFGGLSLALGAALLAGRRREL"
     gene            complement(2897..4489)
                     /locus_tag="P6L01_002229"
     CDS             complement(2897..4489)
                     /locus_tag="P6L01_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDP3031275.1"
                     /translation="MTLLTVNPFDNVGLSALVAAVPIILFLLCLTVFKMKGIYAALTT
                     LVVTLIVALFVFELPARVSAGAITEGVVAGIFPIGYIVLMAVWLYKVSIKTGQFSIIQ
                     DSIASISEDQRIQLLLIGFCFNAFLEGAAGFGVPIAICAVLLIQLGFEPLKAAMLCLI
                     ANGAAGAFGAIGLPVSIIDTFNLSGGVTTLDVARYSALTLPILNFIIPFVLVFIVDGM
                     KGIKEILPVILTVSGTYTGLQLLLTIFHGPELADIIPSLATMVVLAFVCRKFKPKNIF
                     RLEASEHKIQKRTPKEIVFAWSPFVILTAFVLVWSAPFFKKLFQPGGALESLVIKLPI
                     PNTVSDLSPKGIALRLDLIGATGTAILLTVIITILITKLKWKSAGALLVEAIKELWLP
                     ILTISAILAIAKVMTYGGLTVAIGQGIAKAGAIFPLFSPVLGWIGVFMTGSVVNNNTL
                     FAPIQATVAQQISTSGSLLVAANTAGGVAAKLISPQSIAIATAAVKKVGEESALLKMT
                     LKYSIIFVAFICVWTFILTLIF"
BASE COUNT         1777 a          868 c          678 g         1358 t
ORIGIN      
        1 tttatattac tgacttattt atttcatcag caagaaaaca cacttccatt taagaagatt
       61 gtttcagatt tatgttataa acaatcggat ttagtacagc atataaaagt acttgtgaaa
      121 cattcatata ttagtaaagt tcgaagtaaa attgatgagc gtaatactta catttcaata
      181 tctgaagaac aacgagagaa aattgcagaa cgtgttacat tgtttgatca aatcattaaa
      241 caatttaacc ttgcagatca aagtgaatca cagatgatac caaaagatag taaagaattt
      301 ctaaacttga tgatgtatac aatgtatttc aagaatatta tcaaaaaaca tctaacatta
      361 agttttgtag aattcacaat tctagctatt atcacttctc aaaataaaaa catcgttctt
      421 cttaaagatt taattgaaac aatccaccat aaataccctc aaactgttag agctctcaat
      481 aatttaaaaa agcaaggcta tctaataaaa gaacgctcaa ctgaagatga aagaaaaatt
      541 ttaattcata tggatgacgc gcagcaagac catgctgaac aattattagc tcaagtgaat
      601 caattattag cagataaaga tcatttacat cttgtttttg aataatatct ctattacgca
      661 agtgtgctgt attctaaagt gcacttgtgt tttctatttt ttaataaaac ctcagcacat
      721 aatgaacaac tttctatttt ctatatcact taaaaccatt tccgaaatta aacctcagca
      781 cattcaaagc cccactttat tcttaaaaac attttttaac tcatatgtat taaaccgctt
      841 tcattataaa aaatatctct atattttatc tgtttttatt aatcgaaata gcgtgatttt
      901 gcggttttaa gccttttact tcctgaataa atctttcagc aaaatattta ttttataagt
      961 tgtaaaactt acatttaaat ttaattataa atatagattt tagtattgca atacataatt
     1021 cgttatatta tgatgacttt acaaatacat acagggggta ttaatttgaa aaagaaaaac
     1081 atttattcaa ttcgtaaact aggtgtaggt attgcatctg taactttagg tacattactt
     1141 atatctggtg gcgtaacacc tgctgcaaat gctgcgcaac acgatgaagc tcaacaaaat
     1201 gctttttatc aagtgttaaa tatgcctaac ttaaacgctg atcaacgtaa tggttttatc
     1261 caaagcctta aagatgatcc aagccaaagt gctaacgttt taggtgaagc tcaaaaactt
     1321 aatgactctc aagctccaaa agctgatgcg caacaaaata acttcaacaa agatcaacaa
     1381 agcgccttct atgaaatctt gaacatgcct aacttaaacg aagcgcaacg taacggcttc
     1441 attcaaagtc ttaaagacga cccaagccaa agcactaatg ttttaggtga agctaaaaaa
     1501 ttaaacgaat ctcaagcacc gaaagctgat aacaatttca acaaagaaca acaaaatgct
     1561 ttctatgaaa tcttgaatat gcctaactta aacgaagaac aacgcaatgg tttcatccaa
     1621 agcttaaaag atgacccaag ccaaagtgct aacctattgt cagaagctaa aaagttaaat
     1681 gaatctcaag caccgaaagc ggataacaaa ttcaacaaag aacaacaaaa tgctttctat
     1741 gaaatcttac atttacctaa cttaaacgaa gaacaacgca atggtttcat ccaaagctta
     1801 aaagatgacc caagccaaag cgctaacctt ttagcagaag ctaaaaagct aaatgatgca
     1861 caagcaccaa aagctgacaa caaattcaac aaagaacaac aaaatgcttt ctatgaaatt
     1921 ttacatttac ctaacttaac tgaagaacaa cgtaacggct tcatccaaag ccttaaagac
     1981 gatccttcag tgagcaaaga aattttagca gaagctaaaa agctaaacga tgctcaagca
     2041 ccaaaagagg aagacaacaa aaaacctggt aaagaagacg gcaacaaacc tggcaaagaa
     2101 gacggcaaca agcctggtaa agaagacaac aaaaaacctg gtaaagaaga cggcaacaag
     2161 cctggtaaag aagacaacaa caaacctggc aaagaagacg gcaacaagcc tggtaaagaa
     2221 gacaacaaca agcctggtaa agaagacggc aacaaacctg gtaaagaaga cggcaacgga
     2281 gtacatgtcg ttaaacctgg tgatacagta aatgacattg caaaagcaaa cggcactact
     2341 gctgacaaaa ttgctgcaga taacaaatta gctgataaaa acatgatcaa acctggtcaa
     2401 gaacttgttg ttgataagaa gcaaccagca aaccatgcag atgctaacaa agctcaagca
     2461 ttaccagaaa ctggtgaaga aaatccattc atcggtacaa ctgtatttgg tggattatca
     2521 ttagccttag gtgcagcgtt attagctgga cgtcgtcgcg aactataaaa acaaacaata
     2581 cacaacgata gatatcattt tatccaaacc aattttaact tatatacgtt gattaacaca
     2641 ttcttatttg aaatgataag aatcatctaa atgcacgagc aacatctttt gttgctcagt
     2701 gcatttttta ttttacttac ttttctaaac aacttctgaa acgcctcaac actttctact
     2761 ctgattacat atacgacatt tttagacatt aaaaaatcga actagacaag atgctcattg
     2821 catttcgtac tagttcgatt catgaataat tagatttaaa atgtcatttg aatccaagtg
     2881 acaacattat ttatatttag aatattaacg ttagtataaa cgtccaaaca caaataaagg
     2941 caacaaatat aatactgtat tttaacgtca tttttaataa tgcagattct tcaccaactt
     3001 ttttaacagc tgcagtcgca atggcaattg attgtggtga aataagtttc gctgctacac
     3061 cacctgcagt gttagctgcc acaagtaatg aaccgcttgt tgaaatttgt tgtgccactg
     3121 tcgcttgaat aggtgcgaat aaagtattgt tatttacaac tgaaccagtc ataaacacac
     3181 caatccaacc taatactgga gagaataatg ggaaaattgc tcccgcttta gcaatacctt
     3241 gtccaattgc tacagtcaaa ccaccgtatg tcataacttt agcaatagct aggatagctg
     3301 aaattgtaag gatcggtaac cataattctt taattgcttc gaccaataaa gcacctgcac
     3361 ttttccattt taacttcgta attaaaattg taataattac tgttaataaa atcgctgtcc
     3421 cagttgcacc aattaaatcg agacgcaacg caattccttt aggcgataaa tcactcacag
     3481 tatttggaat tggcaatttt attactaaac tttcaagtgc acctccaggt tggaataatt
     3541 ttttgaagaa tggtgcactc catactaata caaaggcagt taaaattacg aacggactcc
     3601 aagcaaagac aatttcttta ggcgttcgtt tttgaatttt atgttcagac gcttccaatc
     3661 tgaaaatgtt tttcggttta aatttacgac aaacaaatgc taacaccacc attgttgcta
     3721 gtgatggaat aatgtctgct agttctggac catggaatat tgttaataat aattgtaatc
     3781 cagtatatgt accactcact gttaaaatga caggtaaaat ttctttaata cctttcatac
     3841 catctacaat gaatactaaa acaaatggaa taataaagtt taaaattgga agtgttaatg
     3901 ctgagtatct cgcaacatct aatgttgtaa cgcctccact taagttaaac gtatcaataa
     3961 tactaactgg taaaccaatt gcaccaaagg cacccgccgc accattagca attaaacata
     4021 acatcgctgc ttttaatggt tcaaatccaa gttgaattaa taatactgca caaatcgcaa
     4081 ttggcacacc aaatcctgct gcaccttcta aaaatgcgtt gaaacaaaat ccaattaata
     4141 atagttggat tctttggtcc tctgaaatac ttgcaatact atcttgaata atagaaaatt
     4201 gtcctgtttt aatagaaact ttatataacc aaactgccat taaaacgata tatcctattg
     4261 ggaaaatacc ggcaacaacg ccttctgtaa tcgcacctgc tgatacacgc gctggtaatt
     4321 caaatacaaa taaagccaca atcaatgtaa caaccaaagt tgtcaatgct gcataaatgc
     4381 ctttcatttt aaaaacggtt aagcataata aaaataaaat aataggtact gctgcaacta
     4441 aggctgataa tccaacatta tcgaatggat ttacagtaag tagtgtcata atgactccct
     4501 ctctttatat aaaatattta tcattctgat taatctacaa cctatttcaa cttatatttt
     4561 gcgatgatca catatttaaa atgtaacact cctatatgtg acaggcaatc gaatttttac
     4621 aaaaagttca caaaatatac acaatattta actataataa ataatatatc attttaatta
     4681 t
//
LOCUS       DAOBBH010000178         4626 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.178, whole genome shotgun sequence.
ACCESSION   DAOBBH010000178 DAOBBH010000000
VERSION     DAOBBH010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.178"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(90..1025)
                     /locus_tag="P6L01_002230"
     CDS             complement(90..1025)
                     /locus_tag="P6L01_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDP3031276.1"
                     /translation="MRYLANNHSALRLKGIILAIVGACLWGLGGTVSDFLFKYKNINV
                     DWYVTARLVVSGVFLLIMYKMMQPKRSIFSVFQDRRMLGKLLIFSILGMLVVQYAYMA
                     SINTGNAAIATLLQYIAPVYIIIWFVIRGVAKLTLFDVLAIIMTLLGTFLLLTNGSFS
                     NLVVNPASLFWGILAGVALAFYTIYPSDLLNRFGSILIVGWAMLISGVAMNLRHPIWH
                     IDITKWDISIILFLIFGIIGGTALAFYFFIDSLQYISAKETTLFGTVEPVVAVIASSL
                     WLHVAFKPFQIVGIILIMILILLLSLKRQPEALDE"
     gene            complement(1208..1411)
                     /locus_tag="P6L01_002231"
     CDS             complement(1208..1411)
                     /locus_tag="P6L01_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031277.1"
                     /translation="MQLSHSVKVAISIYLALIFITFTSYLVIILYTSMTGHDVSHFVL
                     DSQHSHHGSLTQKHLSLPEISFK"
     gene            1961..3004
                     /locus_tag="P6L01_002232"
     CDS             1961..3004
                     /locus_tag="P6L01_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDP3031278.1"
                     /translation="MDILLGVGTLVLVLVIMTLFLKYAPYGKQGLQALSGAACATFLP
                     QAFLSYAIGGVFDIKFLQDIGDLAGSLSGIAVGILTCLNLGVAPVFAVIVGLVLHDFK
                     LLPAFIAAYCVAFLIKWIEKKVPEGLDLIVVILFAPAIAFGLASIITPGVLATLKQIG
                     SAVTAVGDNNPYALAVILGLVIPVTGMTPLSSMVLTSLLGLTGVPMAIGALTCTGSSF
                     VNLILFRKLNIGGPSKAFAVCIEPLTQIDLIAQYPVQLYGTNALIGVVNACIVTFSGL
                     VIGVKGMATPIAGAIVLFGFNNAVTSILTIATVIIVSIVLAYIIGTLINKFNLMNINF
                     KMPSKKNHIKESV"
     gene            3007..3687
                     /gene="sdaAB"
                     /locus_tag="P6L01_002233"
     CDS             3007..3687
                     /gene="sdaAB"
                     /locus_tag="P6L01_002233"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDP3031279.1"
                     /translation="MAKSYDYQSAFDIIGPVMMGPSSSHTAGAVKIGNSARAILGDIP
                     KNIEIHYYESFAQTHQGHGTDVAIVGGAMGYSTFDNRIKSSLEIAADDGIKIDIIEEE
                     GDSIGQHPNCAYIKSSRNDGRYIEIIGISIGGGTIKIKGIHINGLEVDLNHGLPILVV
                     DGNMTKAQVNHFINDINDMKLDCKDEFIKIDEDKCLVVIPLNKAISESTLNTIKEKYS
                     DVNVSYIN"
     gene            3701..4600
                     /gene="sdaAA"
                     /locus_tag="P6L01_002234"
     CDS             3701..4600
                     /gene="sdaAA"
                     /locus_tag="P6L01_002234"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDP3031280.1"
                     /translation="MFDSIRETIDYAVENNMSFADIMVKEEMELSGKSRDEVRAQMKQ
                     NLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATN
                     EVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNAS
                     VAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVE
                     IPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLA
                     GTPTGEAIKRKIFGTAEDMVKNN"
BASE COUNT         1643 a          705 c          886 g         1392 t
ORIGIN      
        1 attaattaaa ataaaaaact ctgatatcac atacaaggta gctacgttgt taaaaatagt
       61 tacttgctaa agtgattatc agagttttct tattcatcta atgcttcagg ttgtctttta
      121 agtgatagta ataaaattaa aatcataata agaatgatgc ctacgatttg aaatggtttg
      181 aatgccacat gtaaccatag actgcttgcg ataacggcta cgacaggttc aacagttccg
      241 aataatgttg tttctttcgc tgatatgtat tgtaaactgt cgataaagaa ataaaatgcg
      301 agtgcggtac caccgataat accaaagatt aaaaataata taattgatat gtcccattta
      361 gtgatatcaa tgtgccaaat tggatggcgt aaattcatcg caacaccaga aataagcatt
      421 gcccacccga caattagaat cgaaccgaag cggttaagta ggtctgaagg ataaattgtg
      481 taaaaagcga gtgctacacc agctaaaata ccccagaata aacttgcagg attgacgact
      541 aaattagaaa atgaaccatt cgttaataat aaaaatgttc ctaatagtgt catgataata
      601 gcaagcacat caaataatgt tagttttgca acgcctctta tgacaaacca aataataata
      661 taaactggcg caatgtattg tagtaatgtt gcaatcgcag cattacctgt attaatagat
      721 gccatataag catattgtac aactaacatg cccagtatac tgaagataag taatttgcct
      781 aacatacgtc gatcttggaa tacgctaaat attgaacgtt tgggttgcat cattttgtac
      841 ataataagta agaaaacacc actgactaca agtcgagcag tgacgtacca atcgacatta
      901 atattcttat atttgaacaa gaaatcagaa acagtaccac ctaatcccca taaacaagca
      961 ccgacaatgg caagtataat tccttttaaa cgcaaagctg aatgattatt cgccaagtat
     1021 ctcacccctt taaaacttaa caccaacaac ataaaatatg tcggtgtatg ataatgattt
     1081 aattcattat atcgactttt aagaaagagt gaaatgagaa aataaaaaaa tcatctaaga
     1141 tgtagtggtc tagatgatag ataaatgaat cgatatggga aatgaatgtt gaaacacaga
     1201 ggataaacta tttaaatgag atttcaggaa gactcaaatg tttttgcgta agagatccat
     1261 gatgagaatg ctgactatct aacacgaaat gtgatacatc atgtccagtc atactcgtat
     1321 ataaaataat gactaaataa gaagtgaacg ttataaagat aagtgctaaa tagatagaaa
     1381 ttgcaacttt aacggaatga ctcaattgca cctttatcgc ctccactaag ttatactaaa
     1441 tattttcatc ttcagtataa ccagccattg ttaagttgat gtaaatttag ctttaaaaaa
     1501 agtaaagatt caacctatag actatcatca ttcctatcag aaaactaaaa ataatatcct
     1561 actttaatta tcttattgca aatatatgtg taataagtta tttttgaata tagtgtgcgg
     1621 ttttataaac acagcacaaa aacaaaaaat aaatagctcg aaagataaag tgtataaata
     1681 ttaaagattt ttagaaatat tactcacttt cagatgtaaa aaagttatca aaaaactgtc
     1741 acacaactgt ttgaaatata gagtaaaaag ttaattagta aatattaatg acaacataag
     1801 tagtttttga aacaatccat tttaaaaatc tttcacaaat atgaaatatt tcacttaaat
     1861 atttgatttg ataacagata aaagttgaaa acgttttctt aagggtgtac gatgtttatg
     1921 gtctgaaaat aaaataaaca tagagagagg tttttgggaa atggatattc tgttaggtgt
     1981 aggcacatta gtcttggttt tagtgatcat gacgctattt ttaaagtatg caccgtacgg
     2041 taaacaagga ctacaagcat tatccggtgc agcttgtgcg acatttctac cacaagcatt
     2101 cttaagttac gctattggtg gcgtatttga tattaaattt ctacaagata ttggggattt
     2161 agcaggaagt ttaagcggta tcgcagtagg tattctaacg tgcttaaact taggcgttgc
     2221 accagtattt gcggttattg ttggtttagt attacatgac tttaaattat taccagcatt
     2281 catcgcagct tattgcgtag cttttttaat taaatggatt gaaaagaaag ttccagaagg
     2341 cttggactta attgtggtga tcttatttgc accagcaatt gctttcggac ttgcatcaat
     2401 cattacacct ggtgtgctag caacattaaa acaaataggt agtgctgtca ctgcggtagg
     2461 tgataacaat ccatatgcat tagcggtcat tttaggacta gtgattcctg ttacaggtat
     2521 gacaccgcta agttcaatgg tattaacaag tttattagga ctgacaggtg ttcctatggc
     2581 tattggagca ctaacatgta caggtagctc atttgttaac cttattttat tccgaaaatt
     2641 aaacattggt ggaccgtcaa aagcatttgc agtttgtatt gagccattga cgcaaattga
     2701 tttaatagcg caatatccag tacaactata tggtacaaat gcactgattg gggtcgtcaa
     2761 cgcatgtatc gtgacattta gcggactagt aataggtgtc aaaggtatgg caacaccaat
     2821 tgcaggtgcc atcgtattgt ttggctttaa taatgcagtg acatcaattc taacaatcgc
     2881 aacagtgatt attgtcagca ttgtcttagc ctatattatt ggaacactaa ttaataagtt
     2941 taatttaatg aatatcaact ttaaaatgcc gagcaaaaag aatcatatta aggagagtgt
     3001 ttaattatgg caaagagcta tgattatcaa agtgctttcg atattatcgg accagttatg
     3061 atgggccctt caagttctca tacggctggt gcagtaaaga ttgggaattc agcacgcgca
     3121 atattgggag atattcctaa aaatattgaa atacattatt atgaatcatt tgctcaaaca
     3181 caccaaggac atggtacaga tgtcgctatt gtgggtggag caatgggata ttcaacattt
     3241 gataaccgaa ttaaatcttc attagaaatt gcagctgatg atggtattaa aatagatatc
     3301 atcgaagaag aaggcgatag tattggtcaa catccaaact gtgcttatat taaatcatct
     3361 cgtaatgatg gtcgctatat tgaaattatc ggtatttcaa tcggtggtgg tacaattaaa
     3421 attaaaggta ttcatataaa tggactagaa gtagatttaa atcatggatt accaatttta
     3481 gttgttgacg gaaatatgac taaagcacaa gtaaatcatt tcattaatga tatcaacgat
     3541 atgaaattag actgtaaaga tgaatttatt aagatagatg aagataaatg cttggttgta
     3601 ataccattaa ataaagcaat ctcagaatcg acattaaata caattaaaga gaaatacagt
     3661 gacgtaaacg tttcctatat aaattagagg gggaataaac atgtttgatt caattagaga
     3721 gactatagat tatgccgtag aaaataatat gtcatttgcg gatatcatgg ttaaagaaga
     3781 aatggaatta agcggtaaat cacgtgatga agtgcgagcg caaatgaaac aaaatttaga
     3841 tgtcatgcga gacgcagtaa tcaaagggac gacaggtgat ggcgttgaaa gtgtaacggg
     3901 ctacactggt catgacgctg ctaaactacg tgattataat gaaacacatc atgctttgtc
     3961 tggatatgaa atgattgatg cagtcaaagg tgccattgca acaaatgaag tcaatgctgc
     4021 gatgggtatt atttgtgcaa cgccaacagc tggttcctcg ggtaccattc ccggtgcact
     4081 ttttaaatta gaaaaaacac atgatttaac agaagagcaa atgattgatt tcttattcac
     4141 ttcagcattg tttgggcgtg tcgtagcaaa caatgcaagt gtagctggtg caacaggtgg
     4201 ctgccaagct gaagttggtt cagcatctgc aatggccgca gctgcagcag tagctatatt
     4261 cggaggatca ccagaagcat ccgggcacgc tatggcatta gcgataagta atttattagg
     4321 tttagtttgt gacccagtag ccggacttgt tgaaattcct tgtgttatga gaaatgcaat
     4381 tggttcgggt aacgctttaa tttcagcaga tttagcatta gcaggtattg aaagtagaat
     4441 acctgttgat gaagttattg aagcaatgga taaggttggt cgtaaccttc ctgcatcatt
     4501 acgtgaaact ggattgggtg gactagcagg cacaccaact ggcgaagcaa ttaaacgtaa
     4561 aatctttggc acagctgaag atatggttaa aaataattaa tgagataaca ataagatatt
     4621 gataaa
//
LOCUS       DAOBBH010000179         4585 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.179, whole genome shotgun sequence.
ACCESSION   DAOBBH010000179 DAOBBH010000000
VERSION     DAOBBH010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.179"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(53..931)
                     /locus_tag="P6L01_002235"
     CDS             complement(53..931)
                     /locus_tag="P6L01_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HDP3031281.1"
                     /translation="MLNIQDVSHLSKKEQKAYNRFVESVENGNLPVLPCIEMDLKEMQ
                     EETLSQSKIGGMPFLKSFKDIPLDENNVPMVLLAQINLNDLPEQQELFPVNEGILQFW
                     ISSEDQMYGMSENLKGNNINSRLVYIKEPITDLSLENIQVHLKSLDADNEDIPFSGAF
                     SIEFRLSKQTITCTDYKYDEDVLALWNKVNPSFALKSMFGGYDELMEPVCNTFTAKEP
                     FNQLGGYPYFDQIDPRTNDQELKMYDRVLLQIDSTRDGNSSIIWGDLGIANILVKSTD
                     LEAMKFDDYMYSWDCS"
     gene            complement(1110..1490)
                     /gene="gcvH"
                     /locus_tag="P6L01_002236"
     CDS             complement(1110..1490)
                     /gene="gcvH"
                     /locus_tag="P6L01_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDP3031282.1"
                     /translation="MAVPNELKYSKEHEWVKVEGNVATIGITEYAQSELGDIVFVELP
                     ETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVK
                     VEISDESQLEALLTAEKYSEMIGE"
     gene            complement(1648..2004)
                     /locus_tag="P6L01_002237"
     CDS             complement(1648..2004)
                     /locus_tag="P6L01_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDP3031283.1"
                     /translation="MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKT
                     IIANTGVEINKLFNTHGAKYRELDLKNKLQTLSDDEKLELLSSDGMLVKRPLAVMGDK
                     ITLGFKEDQYKETWLA"
     gene            2148..2468
                     /locus_tag="P6L01_002238"
     CDS             2148..2468
                     /locus_tag="P6L01_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDP3031284.1"
                     /translation="MQAIKSNESFKSIINSDTPVIVKFEAGWCPDCRAMDLWIDPIVE
                     QYNDYQWYTVNRDELEDVVVENEVMGIPSLLVFKNGDKIAHLHSANAKSPEQVESFLA
                     ETFK"
     gene            complement(2619..3158)
                     /locus_tag="P6L01_002239"
     CDS             complement(2619..3158)
                     /locus_tag="P6L01_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDP3031285.1"
                     /translation="MELQQAIANRRSVKKFKRDMHIDDALLYQAIEKAADAPNHGMRE
                     PWRVVHVPKDRLGNMSKDISKFAFPNELDKQQCHYDAVTKLGGMLLLILKTDPRQRQN
                     DENYFAFGAYAQNLMLLLYEAGIGTCWKSPLYIYDPKVRKTLGIKKDEVLAGFLYLTD
                     LEEDMPKAPRKNRNLITLY"
     gene            complement(3241..3957)
                     /locus_tag="P6L01_002240"
     CDS             complement(3241..3957)
                     /locus_tag="P6L01_002240"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDP3031286.1"
                     /translation="MTHVEVVATIAPQLSIEETLIQKINHRIDAIDVLELRIDQIENV
                     TVDQVAEMITKLKVMQDSFKLLVTYRTKLQGGYGQFINDLYLNLISDLANINGIDMID
                     IEWQADIDIEKHQRIIKHLQQYNKEVVISHHNFESTPPLDELQFIFFKMQKFNPEYVK
                     LAVMPHNKNDVLNLLQAMSTFSDTMDCKVVGISMSKLGLISRTAQGVFGGALTYGCIG
                     EPQAPGQIDVTDLKAQVTLY"
     gene            4105..4527
                     /locus_tag="P6L01_002241"
     CDS             4105..4527
                     /locus_tag="P6L01_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDP3031287.1"
                     /translation="MAIHYETKATNVGGRKGHVYTDDRALDIDIVPPAQADGKATNPE
                     QLFAAGYASCFNGAFDLILKQNKVRDAHPEVTLTVRLEDDSDSESPKLSVSIDATIKN
                     VISQEEAEKYLQMAHEFCPYSKATQGNINVDLNVNVVD"
BASE COUNT         1488 a          821 c          619 g         1657 t
ORIGIN      
        1 gattgatcta tagatactac acttattaaa gtaatatatt tttatgattc tcttagctgc
       61 aatcccatga atacatgtaa tcatcaaact tcatagcctc aaggtcagta gatttcacta
      121 agatattggc aatacctaaa tcaccccata taatcgaaga attaccatct cttgtagaat
      181 caatttgcag taagactcta tcatacattt tcagttcttg atcgttcgtt cttggatcta
      241 tttggtcaaa atatggataa ccaccaagtt gattaaatgg ctccttagca gtaaatgtgt
      301 tacacacagg ttccattaat tcatcatatc caccaaacat cgattttagc gcgaaggatg
      361 gattgacttt attccacaat gcaagcacgt cctcatcgta cttataatca gtacatgtaa
      421 tagtttgttt cgacaatcta aattctatag aaaatgctcc actgaacggg atatcctcat
      481 tatcagcatc taatgacttc aaatgcactt gaatattttc gagtgataaa tctgtaattg
      541 gctcttttat ataaacaagc cttgagttta tattgtttcc ctttaaattt tcagacatac
      601 catacatttg atcttctgaa ctaatccaaa actgcaatat cccttcattt acaggaaata
      661 attcttgttg ttctggaaga tcattcaaat taatctgtgc taacaatacc attggtacat
      721 tattttcatc taatggtata tctttaaaag attttaaaaa tggcattcca ccaatcttac
      781 tctggcttaa agtttcttct tgcatctctt ttaaatccat ttcaatacat ggtagtactg
      841 gtaaattacc gttttctaca gattctacga aacggttata tgctttttgc tcctttttag
      901 aaagatgact aacgtcttga atgtttagca tattaacttc ccccaatttt tatttttaaa
      961 atagctaaat tcattatttt catcattctt gaataataaa atgtagcaat actccaaata
     1021 atagtgccta ataaattttc tttcatatta tttacttact cagactaaaa ataaactcct
     1081 caatcttaga ttaaggagtt acacggtgat tattcaccaa tcatttctga atatttttca
     1141 gctgttaata aagcttcaag ctgactttca tcactaattt ctacttttac catccatgct
     1201 ttttcgtatg gagattcatt tacaaattcg ggactatctt ctagttcttc gttgacttca
     1261 actactttac cagagattgg tgcatataat tctgatacag ttttaactga ttctacgcta
     1321 ccaaacgtat ccccttcatt aatttcatca tctgtttctg gtaattcaac gaaaacaata
     1381 tcacctaact cgctttgtgc gtattctgtg attccaattg ttgctacatt accttcaact
     1441 ttaacccatt catgctcttt tgaatatttc aattcatttg gtactgccaa gagaatcccc
     1501 tcctaattaa tctttacatc attaatgatg ccataaggct agatgctatt caactatatt
     1561 tataaaaatt aacaattgta tagcttattt cattttacat gtaatgaatc taaatatcga
     1621 gatatttagt aaacgtttac atttcactta cgctaaccaa gtctctttat attgatcttc
     1681 tttaaatcct aatgttatct tatcgcccat tactgctaga ggacgcttta ctaacatacc
     1741 atcagatgat aacaactcta acttttcatc atctgataaa gtttgtaatt tatttttcaa
     1801 atcaagctca cgatatttcg caccgtgtgt attaaacaat ttattaattt ctacgcctgt
     1861 atttgcaatt attgttttaa attcatttat tgtaggtgta tgttgaacga tatcaattgg
     1921 ttcataactt acgccatatt catctaaaaa ctttgctgcc tttttacaag ttgtacaatt
     1981 cttatattgg taaaatttaa tcatgcgact gcaacctcct ttatgtattt aaaataaata
     2041 tatcattttc catagcaaaa ggctttcatt ttgaagctaa aattttttat tatgatataa
     2101 atttagctat aataaaaatt attaaactat ctagaggagt tgttacaatg caagcaatca
     2161 aaagtaatga atcatttaaa tctataatta atagcgatac acctgtaatt gttaaatttg
     2221 aggcaggatg gtgcccagac tgtcgtgcta tggatttatg gattgaccca atcgtagaac
     2281 aatataatga ttaccaatgg tatactgtta atcgtgatga attagaagat gtagttgttg
     2341 aaaatgaagt tatgggtatc cctagcttgc tagtatttaa aaacggcgac aaaattgcac
     2401 accttcattc agcaaatgct aagtcacctg aacaagttga atcattttta gcagaaactt
     2461 ttaaataaat actaaaatac aaatgtgtat atacttcaca ataaaaagct gagactgtga
     2521 ttgtaccgac tctagtaaat tagattaaat ctctaactcg atagatgtcc atcaattcat
     2581 gtctcagctt ttattaatgt tttctataat tatacatatt aatataatgt aattaagttt
     2641 ctatttttac gtggtgcttt aggcatatct tcttctaaat ccgttaaata taagaatcca
     2701 gcaagaactt catctttctt tataccaagt gtttttctta ctttaggatc atagatatat
     2761 aatggcgatt tccaacatgt acctatcccc gcttcataaa gtaacaacat aagattttgt
     2821 gcatatgcac caaatgcaaa gtagttttca tcattttgac gttgtcttgg atctgttttt
     2881 aaaataagca ataacatgcc acctagtttc gttactgcat cataatgaca ttgttgctta
     2941 tctaattcat taggaaatgc aaatttagaa atatccttac tcatatttcc taatctgtct
     3001 ttcggaacat gcacaactct ccatggttcc ctcattccgt gatttggagc atcagcagct
     3061 ttctcaattg cttgatatag caatgcgtca tctatgtgca tatctctttt aaattttttc
     3121 acacttcttc tattagctat tgcttgttgt aattccatct aaaatcgctc ctccacgaac
     3181 aatgataata cttctcaatt ataagacagc acttacacaa aagcaaccta attcatttta
     3241 ttagtataaa gtcacttgtg cttttaaatc agtaacatca atctgtcctg gagcttgtgg
     3301 ttctccgata caaccataag tcaatgcacc accaaaaacg ccttgagccg ttctacttat
     3361 tagtccaagt ttagacattg aaataccaac aactttgcag tccatagtat ctgaaaatgt
     3421 agacattgcc tgcaataaat ttaacacatc atttttatta tgtggcatta ctgctaattt
     3481 aacgtattct gggttgaatt tttgcatttt aaaaaatata aattgcaatt catctaatgg
     3541 aggcgtactt tcgaaattat gatgtgatat aaccacctct ttattatact gttgcaagtg
     3601 tttaatgatt cgttgatgtt tttcaatgtc aatatctgct tgccattcta tatcaatcat
     3661 atctatgcca ttgatatttg ctaagtctga tattaaatta agatacaagt catttataaa
     3721 ttgcccatag ccaccttgta actttgtacg atacgtaact aataatttga atgaatcttg
     3781 cataaccttc agctttgtaa tcatttctgc cacttgatca actgtgacat tttcaatttg
     3841 atcaattcgt aattctaata cgtctattgc atcaatacga tgattaattt tttgaattaa
     3901 agtttcttcg atagataatt gtggcgcgat agtcgctact acttccacat gtgtcatagt
     3961 aaatccctca tttcagatac tcattatttt tattgtaaac aacaactttt tatccttcaa
     4021 tcaaacaatg ctataaatga aaatgattaa ggtattttcg aatgggtaaa gcataaatgt
     4081 attttaaatt aggaggttat aagaatggca atacattatg aaactaaagc gacgaatgtt
     4141 ggcggacgta aaggacatgt ttatactgat gatcgagcgt tagatattga tatcgttccg
     4201 cctgctcaag cagatggtaa agctactaac cccgaacaat tatttgcagc aggttatgca
     4261 tcttgcttca acggtgcttt cgacctaatt ttaaagcaaa acaaagtgcg tgatgctcat
     4321 ccagaagtaa cactaacagt gagactagaa gatgattcag actcagaaag tcctaaatta
     4381 agtgtttcaa ttgatgcgac aattaaaaat gttatatctc aagaagaagc tgaaaaatat
     4441 ttacaaatgg ctcatgaatt ttgtccatat tcaaaagcga ctcaaggaaa tattaatgtc
     4501 gatttaaatg taaatgttgt agattagcat taacttaaag agattattca acgttattaa
     4561 taaaattcac ataaaattca aattt
//
LOCUS       DAOBBH010000180         4582 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.180, whole genome shotgun sequence.
ACCESSION   DAOBBH010000180 DAOBBH010000000
VERSION     DAOBBH010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.180"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            151..759
                     /locus_tag="P6L01_002242"
     CDS             151..759
                     /locus_tag="P6L01_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDP3031288.1"
                     /translation="MSKFDEQIIVVPREIIFNNEKNTFNGFLNKNKPEGQNIFDALSQ
                     YEVKRRGDMEEDPSYKQLISYCLLENEHGEILVYERLSGGGEARLHGQSSIGVGGHMN
                     DVPGAESINEVLRVNAQRELEEEVGLSEQDSQNMEYIGFINDDNNEVGKVHIGVVFKI
                     TVSTNDVEAKETDTLRIKWVEKGNIESYDDFETWSALILQDL"
     gene            778..1707
                     /locus_tag="P6L01_002243"
     CDS             778..1707
                     /locus_tag="P6L01_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031289.1"
                     /translation="MSDIIQFPNSSKKLYKDIKRAEQDQNYDLMYEYIVQYERQFELT
                     EEIAMMKCRMLYETGSFLELREETIVLLKTGIQQYDALMIYYVKSLIGLGQYFEAVEV
                     IHQIIDEVKDHKTRMALHPLKEFAKSKLIEDEKRLTQSLTDFDTLSMREQTHLILKLI
                     DNGHFQFQETVLYILKSNTYSYNLISLMIEYLRFANCTQELTIEKYGMDVTFVPANLN
                     GLEHTTLKEKVIPNVMETLNDGALHIAEEAHHIMNNHSIMIYPIDIETLFETNKWINA
                     YECYFKNMLGLKCELQSIDAFNFIQQLDLNNNS"
     gene            1712..1822
                     /locus_tag="P6L01_002244"
     CDS             1712..1822
                     /locus_tag="P6L01_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031290.1"
                     /translation="MQNNTQHFNIVLTKKVDLQIYKRCDFNVCIGNRPMI"
     gene            1870..3171
                     /locus_tag="P6L01_002245"
     CDS             1870..3171
                     /locus_tag="P6L01_002245"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDP3031291.1"
                     /translation="MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGF
                     RKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGK
                     DFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVV
                     ENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVT
                     FPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRL
                     AEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTY
                     FQISGQDETQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNI
                     SVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED"
     gene            3322..>4582
                     /gene="clpX"
                     /locus_tag="P6L01_002246"
     CDS             3322..>4582
                     /gene="clpX"
                     /locus_tag="P6L01_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDP3031292.1"
                     /translation="MFKFNEDEENLKCSFCGKDQDQVKKLVAGSGVYICNECIELCSE
                     IVEEELAQNTSEAMTELPTPKEIMDHLNEYVIGQEKAKKSLAVAVYNHYKRIQQLGPK
                     EDDVELQKSNIALIGPTGSGKTLLAQTLAKTLNVPFAIADATSLTEAGYVGDDVENIL
                     LRLIQAADFDIDKAEKGIIYVDEIDKIARKSENTSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPNQEMIQIDTTNILFILGGAFDGIEEVIKRRLGEKVIGFSSNEADKYD
                     EQALLAQIRPEDLQAYGLIPEFIGRVPIVANLETLDVTALKNILTQPKNALVKQYTKM
                     LELDDVDLEFTEEALSAISEKAIERKTGARGLRSIIEESLIDIMFDVPSNENVTKVVI
                     TAQTINEETEPELYDAEGNLINNSKTSA"
BASE COUNT         1783 a          594 c          874 g         1331 t
ORIGIN      
        1 ttaaaataat aacacttata aaaaagagat ggcattgtca tctctttttt attttttaaa
       61 aagcaaaatg ccacatttaa tcgctaaaat atgacatatt tgctaaaatg catttaagag
      121 aacaaacatt aaagtgagga gttttatacg atgtctaaat ttgatgaaca aatcattgta
      181 gtacctagag aaattatttt taataatgaa aagaatactt tcaatgggtt tttaaataag
      241 aataaaccag aaggtcaaaa tatttttgat gcgctaagtc aatatgaagt taagcgacgc
      301 ggcgatatgg aagaggatcc atcatataaa caactcattt cttattgttt acttgaaaat
      361 gagcatggcg agatattagt gtatgaacga ttatctggcg gtggagaagc tcgattgcat
      421 ggacaatctt caataggtgt aggcggtcat atgaatgatg ttccaggagc agaatctatt
      481 aacgaagtat tgagagttaa tgcacagaga gaattagaag aagaagtagg tttaagtgag
      541 caagattcac aaaatatgga atatatcggt tttattaatg acgataataa tgaagtgggc
      601 aaggtacata ttggtgttgt atttaaaatc actgtaagta cgaatgatgt agaagctaaa
      661 gaaacagata ctttacgaat aaaatgggtt gaaaaaggca acatagagtc atatgatgat
      721 ttcgaaacgt ggagcgcatt aatccttcaa gatttataat caaacgaggt gacatatatg
      781 tcagatatta ttcagtttcc taactcttcg aaaaagttat ataaagatat aaaacgtgct
      841 gaacaagacc aaaactatga cctgatgtat gaatatattg tgcaatatga acgtcaattt
      901 gaactcactg aagaaatagc aatgatgaaa tgccgtatgt tatatgagac ggggtcattt
      961 cttgaattaa gagaagaaac aattgtctta ttgaaaactg gcatacaaca atatgatgca
     1021 ttgatgattt attacgtgaa aagtttgatt ggtttgggac aatattttga agcggtagaa
     1081 gtaattcatc aaattattga tgaagtcaaa gatcacaaaa caagaatggc attacatcct
     1141 cttaaagaat ttgctaagtc aaaattaatt gaagatgaaa aacgattgac tcagtcatta
     1201 actgattttg atacgttatc aatgagggaa cagacgcact tgattttgaa gttaatagac
     1261 aatggtcatt ttcaatttca agaaacggta ttatatatat taaaatctaa tacgtacagt
     1321 tataacctca ttagtttaat gattgagtat ttaaggtttg caaattgtac acaagaactg
     1381 acaattgaaa agtatggtat ggatgtaact tttgtaccag ctaatttaaa tgggctagaa
     1441 catacaacac tgaaagaaaa agttatacct aacgttatgg aaacattaaa tgatggtgca
     1501 ttacatatcg ctgaagaagc acaccatatt atgaataatc actctatcat gatttatcca
     1561 attgatattg aaactttgtt tgaaacgaat aaatggatta atgcatacga atgttatttc
     1621 aaaaatatgt taggacttaa atgtgaatta caaagtatcg atgcatttaa ttttattcag
     1681 caattagatt tgaataataa tagttaaatt gttgcaaaat aatactcagc attttaatat
     1741 agttttgacg aaaaaagttg atttacaaat atataaacgt tgtgatttca atgtttgtat
     1801 agggaataga cctatgatat aatagggaag ttgaaaaaat aaattagtta atcatggagg
     1861 tatatataca tgacagcaac ttgggaaaaa aaggaaggta acgaaggttt attaactgtt
     1921 actgttcctg cagaaaaagt aaacaaagcg ttagatcaag cattcaaaaa agtggttaaa
     1981 caaattaacg tacctggatt ccgtaaaggt aaagtgccac gtccaatttt tgaacaacgc
     2041 tttggtgtag aagcattata tcaagatgct atcgacattt tattaccaga tgcttatggt
     2101 gaagcaattg acgaaactga tattaaacca gttgcacaac cagaagtaag tgttactcaa
     2161 attgaaaaag gtaaagattt catttttgaa gcaacagtta cagttgagcc agaagttaaa
     2221 ttaggagact ataaaggtct tgaaattgaa aaacaagaaa ctgaattatc tgatgatgag
     2281 ttacaagaag cgattgacca cagcttagga catttagctg aaatggtagt taaagaagat
     2341 ggtgttgttg aaaatggcga cacagttaac attgacttta gtggttcagt tgacggagaa
     2401 gaattcgaag gtggacaagc tgaaggttac gatttagaaa tcggttcagg ttcattcata
     2461 cctggtttcg aagagcaatt agaaggtatg aaagttgacg aagaaaaaga tgttgtcgta
     2521 acattcccag aagaatacca tgctgaagaa ttagctggta aagaagcaac tttcaaaaca
     2581 aaagttaacg aaattaaatt taaagaagtt ccagaattaa cagatgaaat tgctaatgaa
     2641 ttagatgcag aagcaaatac agtagacgag tacaaagaaa acttacgtaa acgtttagct
     2701 gaacaaaaag ctacagatgc tgaaaatgtt gaaaaagaag aagcgattac aaaagctact
     2761 gataatacaa caatcgatat tcctgaagca atggttaata ctgaattaga tcgtatggtg
     2821 tctgaatttg cacaaagaat tcaacaacaa ggtttagatt tacaaacgta cttccaaatc
     2881 tcaggtcaag atgaaactca attaagagag caaatgaaag acgatgcaga acaacgtgtt
     2941 aaaactaact taactttaac tgcgatcgct gaagctgaaa aaatcgaagc tactgatgaa
     3001 gatatcgata aagaattaga aaaaatgagt aaacaattta atatctcagt tgaagatatc
     3061 aaaaatactt taggtaatac tgatatcatt aaaaatgatg ttcgtatcca aaaagttatc
     3121 gatttattaa gagataacgc aaagttcgtt gaaggaacta aagaagatta atcttcatta
     3181 aatattaaat tacaaaaatg agtagcagat gcatagctta tgtatctgct actattcttt
     3241 aagcaaaaag tttgtatgtt aatatgttgc attgtaatat ccaatctagt atagtcttta
     3301 acgaataggg gtgtaaaaag aatgtttaaa ttcaatgaag atgaagaaaa tttgaaatgc
     3361 tctttctgcg gaaaagacca agatcaagta aaaaaacttg tagcaggaag tggtgtatat
     3421 atttgtaatg agtgtattga attatgctca gaaatcgtcg aagaagaatt agctcaaaac
     3481 acttctgaag cgatgacaga attacctact cctaaagaaa ttatggatca tttaaacgaa
     3541 tatgttattg gtcaagaaaa agctaaaaaa tctttagctg tagctgttta taaccactat
     3601 aagcgtattc aacaattagg accaaaagaa gatgatgttg aattacaaaa aagtaacatt
     3661 gcattaattg ggccaacagg tagtggtaaa acattattag ctcaaacatt agccaagacg
     3721 ttgaatgtac catttgcaat tgcagatgcg acaagtttaa ctgaagctgg ttatgtaggc
     3781 gatgatgttg aaaatatctt gttgagatta attcaagcag ctgactttga cattgataaa
     3841 gccgaaaaag gtattattta tgtagatgaa attgataaaa ttgcacgtaa atctgaaaac
     3901 acatctataa cacgtgacgt ttcaggtgaa ggtgttcaac aagcattgct taaaatctta
     3961 gaaggtacga ctgcaagtgt tccgccacaa ggtggacgca aacatccaaa ccaagaaatg
     4021 attcaaattg atacaacaaa tatcttattt attcttggtg gtgcctttga tggtattgaa
     4081 gaagtgatta agcgccgtct tggtgaaaaa gttattggtt tctcaagcaa tgaagctgat
     4141 aaatatgacg aacaagcatt attagcacaa attcgcccag aagatttgca agcctatggt
     4201 ttgattcctg aatttatcgg acgtgtgcca attgtagcta atttagaaac attagatgta
     4261 actgcgttga aaaacatctt aacgcaacct aaaaatgcac ttgtgaaaca atatactaaa
     4321 atgctggaat tagatgatgt ggatttagag ttcactgaag aagctttatc agcaattagt
     4381 gaaaaagcaa ttgaaagaaa aacaggtgcg cgtggtttac gttcaatcat agaagaatcg
     4441 ttaatcgata ttatgtttga tgtgccttct aacgaaaatg taacgaaggt agttattaca
     4501 gcacaaacaa ttaatgaaga aactgaacca gaactatacg acgcagaagg caatttaatt
     4561 aataatagta aaacatcagc tt
//
LOCUS       DAOBBH010000181         4559 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.181, whole genome shotgun sequence.
ACCESSION   DAOBBH010000181 DAOBBH010000000
VERSION     DAOBBH010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.181"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            79..4236
                     /gene="sdrD"
                     /locus_tag="P6L01_002247"
     CDS             79..4236
                     /gene="sdrD"
                     /locus_tag="P6L01_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrD"
                     /protein_id="HDP3031293.1"
                     /translation="MLNRENKTAITRKGMVSNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAESTNKELNEATTSASDNQSSDKVDMQQLNQEDNTKNDNQKEMVSSQGNE
                     TTSNGNKSIEKESVQSTTGNKVEVSTAKSDEQASPKSTNEDLNTKQTISNQEGLQPDL
                     LENKSVVNVQPTNEENKKVDAKTESTTLNVKSDAIKSNAETLVDNNSNSNNENNADII
                     LPKSTAPKSLNTRMRMAAIQPNSTDSKNVNDLITSNTTLTVVDADNSKTIVPAQDYLS
                     LKSQITVDDKVKSGDYFTIKYSDTVQVYGLNPEDIKNIGDIKDPNNGETIATAKHDTA
                     NNLITYTFTDYVDRFNSVKMGINYSIYMDADTIPVDKKDVPFSVTIGNQITTTTADIT
                     YPAYKEADNNSIGSAFTETVSHVGNVEDPGYYNQVVYVNPMDKDLKGAKLKVEAYHPK
                     YPTNIGQINQNVTNIKIYRVPEGYTLNKGYDVNTNDLVDVTDEFKNKMTYGSNQSVNL
                     DFGDITSAYVVMVNTKFQYTNSESPTLVQMATLSSTGNKSVSTGNALGFTNNQSGGAG
                     QEVYKIGNYVWEDTNKNGVQELGEKGVGNVTVTVFDNNTNTKVGEAVTKEDGSYLIPN
                     LPNGDYRVEFSNLPKGYEVTPSKQGNNEELDSNGLSSVITVNGKDNLSADLGIYKPKY
                     NLGDYVWEDTNKNGIQDQDEKGISGVTVTLKDENGNVLKTVTTDADGKYKFTDLDNGN
                     YKVEFTTPEGYTPTTVTSGSDIEKDSNGLTTTGVINGADNMTLDSGFYKTPKYNLGNY
                     VWEDTNKDGKQDSTEKGISGVTVTLKNENGEVLQTTKTDKDGKYQFTGLENGTYKVEF
                     ETPSGYTPTQVGSGTDEGIDSNGTSTTGVIKDKDNDTIDSGFYKPTYNLGDYVWEDTN
                     KNGVQDKDEKGISGVTVTLKDENDKVLKTVTTDENGKYQFTDLNNGTYKVEFETPSGY
                     TPTSVTSGNDTEKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKDVKVILLNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPTGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDAGKHTPVKPMSTTKDHHNKAKALPETGNENSGSNNATLFGGLFAALGSLLLFG
                     RRKKQNK"
BASE COUNT         1850 a          684 c          810 g         1215 t
ORIGIN      
        1 gtaatactat attgatatac atttttgtat ttaaaacaat tgtattattt aaaaatttga
       61 tgaattagga gtaatctaat gctaaacaga gaaaataaaa cggcaataac aagaaaaggc
      121 atggtatcca atcgattaaa taaattttcg attagaaagt acacagtggg aacagcatca
      181 attttagtag gtacaacatt aatttttggt ctggggaacc aagaagcaaa ggctgcagaa
      241 agtactaata aagaattgaa cgaagcgaca acttcagcaa gtgataatca atcgagtgat
      301 aaagttgata tgcagcaact aaatcaagaa gacaatacta aaaatgataa tcaaaaagaa
      361 atggtatcat ctcaaggtaa tgaaacgact tcaaatggga ataaatcaat agaaaaagaa
      421 agtgtacaat ctaccactgg aaataaagtt gaagtttcaa ctgccaaatc agatgagcaa
      481 gcttcaccaa aatctacgaa tgaagattta aacactaaac aaactataag taatcaagaa
      541 gggttacaac ctgatttgct agagaataaa tcagtggtaa atgttcaacc aactaatgag
      601 gaaaacaaaa aggtagatgc gaaaactgaa tcaactacat taaatgttaa aagtgatgct
      661 atcaagagta atgctgaaac tcttgttgat aacaatagta attcaaataa tgaaaataat
      721 gcagatatca ttttgccaaa aagtacagca cctaaaagtt tgaatacaag aatgcgtatg
      781 gcagcaatac aaccaaactc aacagattct aaaaatgtta atgatttaat cacatcaaat
      841 acaacattaa ctgtcgttga tgcagataat agcaaaacga ttgtaccagc ccaagattat
      901 ttatcattaa aatcacaaat tacagttgat gacaaagtta aatcaggtga ttatttcaca
      961 attaaatact cagatacagt acaagtatat ggattgaatc cggaagatat taaaaatatt
     1021 ggtgatatta aagatccaaa taatggtgaa acaattgcga ctgcaaaaca tgatactgca
     1081 aataatttaa ttacatatac atttacagat tatgttgatc gatttaattc agtaaaaatg
     1141 ggtattaatt actcaattta tatggatgca gatacaattc ctgttgacaa gaaagatgtt
     1201 ccttttagtg taactattgg aaatcaaatt acaactacaa cagcagatat cacttatccg
     1261 gcttataaag aagctgacaa taattcaata ggatcagctt ttacagagac agtttctcat
     1321 gtaggaaatg ttgaagaccc tggttactat aaccaggtag tatatgttaa tcctatggat
     1381 aaggatttaa aaggtgctaa gttaaaagtt gaagcgtacc atccgaaata tccaactaat
     1441 attggtcaaa ttaatcaaaa tgttacaaat ataaaaatat atcgtgttcc tgaaggatat
     1501 acattgaata aaggatatga cgttaatact aatgatttgg tagacgtaac tgatgaattt
     1561 aaaaataaaa tgacgtatgg atcaaatcaa agtgttaatc ttgattttgg tgatattaca
     1621 tcagcatatg ttgtaatggt taatacaaaa ttccaatata caaatagcga aagcccaaca
     1681 cttgttcaaa tggctacttt atcttcaaca ggtaataaat ccgtttctac tggcaatgct
     1741 ttaggattta ctaataacca aagtggcgga gctggtcaag aagtatataa aattggtaac
     1801 tacgtatggg aagatactaa taaaaacggt gttcaagaat taggagaaaa aggcgttggc
     1861 aatgtaactg taactgtatt tgataataat acaaatacaa aagtaggaga agcagttact
     1921 aaagaagatg ggtcatactt gattccaaac ttacctaatg gagattaccg tgtagaattt
     1981 tcaaacttac caaaaggtta tgaagtaacc ccttcaaaac aaggtaataa cgaagaatta
     2041 gattcaaacg gcttatcttc agttattaca gttaatggca aagataactt atctgcagac
     2101 ttaggtattt acaaacctaa atacaactta ggtgactatg tctgggaaga tacaaataaa
     2161 aatggtatcc aagaccaaga tgaaaaaggt atatctggcg taacggtaac attaaaagat
     2221 gaaaacggta acgtgttaaa aacagttaca acagacgcag atggcaaata taaatttact
     2281 gatttagata atggtaatta taaagttgaa tttactacac cagaaggcta tacaccgact
     2341 acagtaacat ctggtagcga cattgaaaaa gactctaatg gtttaacaac aacaggtgtt
     2401 attaatggtg ctgataacat gacattagat agtggattct acaaaacacc aaaatataat
     2461 ttaggtaatt atgtatggga agatacaaat aaagatggta agcaggattc aactgaaaaa
     2521 ggtatttcag gcgtaacagt tacattgaaa aatgaaaacg gtgaagtttt acaaacaact
     2581 aaaacagata aagatggtaa atatcaattt actggattag aaaatggaac ttataaagtt
     2641 gaattcgaaa caccatcagg ttacacacca acacaagtag gttcaggaac tgatgaaggt
     2701 atagattcaa atggtacatc aacaacaggt gtcattaaag ataaagataa cgatactatt
     2761 gactctggtt tctacaaacc gacttacaac ttaggtgact atgtatggga agatacaaat
     2821 aaaaacggtg ttcaagataa agatgaaaag ggtatttcag gtgtaacagt tacgttaaaa
     2881 gatgaaaacg acaaagtttt aaaaacagtt acaacagatg aaaatggtaa atatcaattc
     2941 actgatttaa acaatggaac ttataaagtt gaattcgaga caccatcagg ttatacacca
     3001 acttcagtaa cttctggaaa tgatactgaa aaagattcta atggtttaac aacaacaggt
     3061 gtcattaaag atgcagataa catgacatta gacagtggtt tctataaaac accaaaatat
     3121 agtttaggtg attatgtttg gtacgacagt aataaagacg gcaaacaaga ttcaactgaa
     3181 aaaggtatca aagatgttaa agttatttta ttaaatgaaa aaggcgaagt aattggaaca
     3241 actaaaacag atgaaaatgg taaataccgc tttgataatt tagatagcgg taaatacaaa
     3301 gttatttttg aaaagcctac tggcttaaca caaacaggta caaatacaac tgaagatgat
     3361 aaagatgccg atggtggcga agttgatgta acaattacgg atcatgatga tttcacactt
     3421 gataatggct actacgaaga agaaacatca gatagcgact cagattcgga cagcgattca
     3481 gactcagaca gcgattcaga ctcagatagt gattcagatt cagatagtga ttcagattca
     3541 gatagtgatt cagattcaga cagcgactca gactcagata gtgactcaga ctcagatagc
     3601 gattcagatt cagatagcga ttcagactca gacagcgatt cagattcaga cagcgactca
     3661 gactcagata gcgactcaga ttcggacagc gattcagact cagatagcga ctcagactca
     3721 gacagcgatt cagactcaga tagcgactca gactcagata gcgattcaga ttcagacagc
     3781 gattcagatt cagacagtga ttcagattca gacagcgact cagattcaga tagcgattca
     3841 gactcagact cagatagcga ttcagattca gacagcgact cagattcgga cagcgactca
     3901 gactcagaca gtgattcaga ttcagatagc gactcagact cagatagcga ctcagattca
     3961 gacagcgatt cagactcaga tagtgactca gattcggaca gcgattcaga ctcagatagc
     4021 gactcagatt cagacagtga ttcagactca gatgcaggta agcacacacc tgttaaacca
     4081 atgagtacta ctaaagacca tcacaataaa gcaaaagcat taccagaaac aggtaatgaa
     4141 aatagtggct caaataacgc aacgttattt ggcggattat tcgcagcatt aggatcatta
     4201 ttgttattcg gtcgtcgtaa aaaacaaaat aaataataca gcttatacca ggtccgtgag
     4261 gcctggtttt ttatttcaaa atttatgaaa tgtatcttat gtttgagatt aaatagtgca
     4321 attttttaaa aaatataaca taaatcaaca taaatttaat tcatctttac attaaaagta
     4381 caaacgaata agaaaagcaa ttaaacacag tatcaaacca tttaaaacaa ttgatataaa
     4441 aaacaaacga ataattaaaa attactagaa ttaatttatt caaaccaaag tgtaaaataa
     4501 cagtcttaga taaataaatt tatttaaagt attgtgcttt atctaaaaat gtattacga
//
LOCUS       DAOBBH010000182         4545 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.182, whole genome shotgun sequence.
ACCESSION   DAOBBH010000182 DAOBBH010000000
VERSION     DAOBBH010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.182"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(140..553)
                     /locus_tag="P6L01_002248"
     CDS             complement(140..553)
                     /locus_tag="P6L01_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031294.1"
                     /translation="MKPTKVILRDASYLHSKTSITFILKDVVIEEDNKIYYFDTSATF
                     EDQEVKFEFALFDSDMDNLKHMDYDNPVTEIYFIEPDLHFTIIDFNQELLCIYIDFDS
                     GLRHSNMATESGISLRINIAKSDFTKFINELASLY"
     gene            complement(582..692)
                     /locus_tag="P6L01_002249"
     CDS             complement(582..692)
                     /locus_tag="P6L01_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031295.1"
                     /translation="MDGNNQIDVCFFVLVNNYYTMSIYPRIINSNGDYAT"
     gene            complement(778..1509)
                     /locus_tag="P6L01_002250"
     CDS             complement(778..1509)
                     /locus_tag="P6L01_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDP3031296.1"
                     /translation="MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINE
                     TEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGK
                     KYITPDGTELEGLGILDFYTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHDFGTL
                     GHVTFGYGNNDEDKKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPK
                     EIDNEAEIQAKQVLIDRANRQKKSR"
     gene            complement(1511..2824)
                     /gene="murT"
                     /locus_tag="P6L01_002251"
     CDS             complement(1511..2824)
                     /gene="murT"
                     /locus_tag="P6L01_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HDP3031297.1"
                     /translation="MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDVLRKLA
                     EQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKT
                     KIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKL
                     LLNADDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQ
                     IGHYHCQCGFKREQAKYEISSFDVAPFLYLNINDEKYDMKIAGDFNAYNALAAYTVLR
                     ELGLNEQTIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEK
                     VYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPI
                     IVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS"
     gene            3116..3616
                     /gene="ftnA"
                     /locus_tag="P6L01_002252"
     CDS             3116..3616
                     /gene="ftnA"
                     /locus_tag="P6L01_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type ferritin FtnA"
                     /protein_id="HDP3031298.1"
                     /translation="MLSKNLLEALNDQMNHEYFAAHAYMAMAAYCDKESYEGFANFFI
                     QQAKEERFHGQKIYNYINDRGAHAEFRAVSAPKIDFSSILETFKDSLSQEQEVTRRFY
                     NLSEIARQDKDYATISFLNWFLDEQVEEESMFETHINYLTRIGDDSNALYLYEKELGA
                     RTFDEE"
     gene            complement(3698..3790)
                     /locus_tag="P6L01_002253"
     CDS             complement(3698..3790)
                     /locus_tag="P6L01_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031299.1"
                     /translation="MTKNTIISLENEKTQINDSENESSDLRKAK"
     gene            4006..>4545
                     /locus_tag="P6L01_002254"
     CDS             4006..>4545
                     /locus_tag="P6L01_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDP3031300.1"
                     /translation="MIQDAFVALDFETANGKRTSICSVGMVKVIDSQITETFHTLVNP
                     QDYFSQQNIKVHGIQPEDVENAPTFDYVFPYMMQFIADLPVVAHNAAFDMNVLHQSIQ
                     NIGLPTPNLTYFCSYQLAKRTVDSYRYGLKHMMEFYQLDFHGHHDALNDAKACAMITF
                     RLLKNYENLTYVTNIYGKNL"
BASE COUNT         1495 a          798 c          609 g         1643 t
ORIGIN      
        1 gcctcttttt atcgtatata gcatgtccct ttttaaaatt acaagcataa atattcagac
       61 tcaaatatag ccacacgcct aaactacgga gggatgtggc tgtctttttt gattgtaatt
      121 atgtatcaga tttaaatcat taatataaag aggctaattc attaataaat ttagtaaaat
      181 ctgatttagc aatatttatc cttaatgaaa taccagattc tgttgccatg tttgaatgcc
      241 ttaaaccaga atcaaaatca atataaatac aaagcagttc ttgattaaaa tcaataattg
      301 taaaatgtaa atcaggttct ataaaatata tttcagtaac aggattgtcg taatccatat
      361 gtttcaaatt gtccatgtca ctatcaaaaa gtgcaaattc aaatttaact tcttgatctt
      421 cgaaagttgc actagtgtcg aaataataaa ttttattatc ttcttcgata actacatctt
      481 ttaaaataaa tgttatcgat gttttgctat gtaaataaga tgcatctctt aaaatcactt
      541 tagtgggctt catcgtcaaa ttcctttcat cattcatttt ttcatgttgc ataatcacca
      601 ttactattga taattctagg atagatactc attgtataat aattattcac tagaacaaaa
      661 aaacacacat caatttggtt gtttccatcc aaaataatgt gtgtgtttat acatatatga
      721 tttattacta aacttcatcg tttatcaata ttaaatccat tgatgcgatg ttcagagtta
      781 acgagatttc ttctgtctat ttgctctgtc tattaatact tgtttcgctt gtatttccgc
      841 ttcattatct atttctttag gctcaaacgg aatcccctta cgttcacaag ctttttctaa
      901 cagataatca gtgatttcgt aatttttagg taaaattggt ccgtgtaaat aagtacctaa
      961 taaattttta taatgaatgc cttctttttt atcttcatca ttattaccat aaccaaaagt
     1021 aacatgacca agtgtaccga aatcatgata tgttctacca ccgtgatttt caaaacctac
     1081 aatagttcca aaagtatcac tttcgataac aatatctcct gttaatcggt ttgtctttga
     1141 ttcagtataa aaatctaaaa tacctaaccc ttctaattct gtaccatcag gcgtgatata
     1201 tttttttcct aaaaattgat agcctccaca aattgttaat cccggcatac catcttcaat
     1261 cgcttcttta agtggtgtct taattttact taattctttt gttgctaatg cttgttctct
     1321 atcacttcca ccaccgataa agaaaatatc acattcatca aaggtaatac cttctgtttc
     1381 attgatttct acgacattaa ctttaatatt tcgtttttta gcacgttgtc ttaaagcaat
     1441 aatatttcct atatcactgt ataaattcaa tttatctgac ataaaatgat aaatagtcaa
     1501 ttcatgcata ttatgattga cctccttcaa acgaacggtt taattgttca agcataggcg
     1561 ctaatgatgt atagtttggt attgcaactg tgaaaccttt ataatccata gtctttgccg
     1621 ttgctttata aatatcacgc tcaactataa ttggtacttc aacctctgct aacttcaatc
     1681 gcaattgaag ttcttctgct cgtgtacctg tcacgatgat agcttcaatt tgttgcttag
     1741 ataatttttc aaaatctgca tcataaatcc atgaagtatc tcgaccatct gcagcgttat
     1801 catttagcga aataacatac actttttcgc cttctaattg ttcaccaact gataaacttg
     1861 cattcattcc tgcaggattt ttagctaaat tgatcatcgc ttcttttcgt tcttttttaa
     1921 agtactgcat acgaccattg tctgatgtat acgtttcaaa gccattttta attgtttgtt
     1981 catttaaccc tagctctctt aaaacagtat atgctgctaa cgcgttataa gcgttaaagt
     2041 cacctgcaat tttcatatca tatttttcat cattgatatt taaatataaa aacggtgcca
     2101 catcaaaact tgatatttca tattttgctt gctctcgttt gaaaccacac tgacagtgat
     2161 aatgaccaat ttgattataa tgaatataat cgtattgcaa taagcgacca cagtttggac
     2221 aatatctact ttcattcatc gtactttgtt caaattcatg ggcatgtgct ttcataccat
     2281 agtacacaat cgtatcactt gcgattttca aacgactcac aaatggatca tcagcattta
     2341 gcaataattt gatgccttta ttactaattg tctctgcaat gttattaacc ataatatcaa
     2401 tttcaccgaa gcgatccatt tgatctctaa agaaattagt aaataccatc attgaaggtg
     2461 taacttcttt taacacacgt ggaatcgaac cttcatcaat ttcgattacc gcaattttag
     2521 tcttaggtgt tgattgcatg atgaatgcag aagttatacc tgcagccata ttagcacctt
     2581 cattattgtg tataatttga atattatttg cttttaaagt atgtccaatt aagtttgaag
     2641 tcgttgtttt accatttgtt ccactgataa atacaatatc atcaacttgc tctgctaatt
     2701 ttcttaatac atctgtatcc acttttctag cgatttgtcc aggtaaatct gttcctcttt
     2761 tacctactgc tctacttgct ttacgcgcca atttcgctag atggattgcc gtccactgtc
     2821 tcatgtgttt cctcctcaaa tttccactcg catcattata acatgacaag gcaacttcaa
     2881 aaaagtttct caatcacaaa ttgatacagt gtttctaaat tataatcatt ctcatttaga
     2941 taattaaaaa tgatatttat tctcaattaa atatcgctaa atgatacatt ttctcaatta
     3001 gagtgctaaa ataaggtatt aaattataat tattattatt tacaattgag aatgagtttg
     3061 ttatactaag tttaattcat agtaatttta atttacaaat aagaggtgta tcaaaatgtt
     3121 aagtaaaaat ttattagaag cattaaatga tcaaatgaac catgagtact ttgcagcaca
     3181 cgcatatatg gcaatggcag catactgtga taaagaatcg tacgaaggat ttgcaaactt
     3241 cttcattcaa caagctaaag aagaacgttt ccatggacaa aagatttata actatattaa
     3301 cgacagaggt gcacatgcag aattcagagc agtttcagca ccaaaaattg acttttcaag
     3361 catactagaa actttcaaag acagcttatc tcaagaacaa gaagtaacaa gacgtttcta
     3421 taacttatct gaaatcgctc gtcaagataa agattatgca actatctcat tcttaaactg
     3481 gttcttagat gaacaagtcg aagaagaatc aatgtttgaa actcacatca attatttaac
     3541 tcgtatcggc gatgacagca atgcattata tctttacgaa aaagaacttg gcgctcgtac
     3601 attcgacgaa gaataattaa acatcactac aatagacaga taaatatcat acgacatgat
     3661 aggcatttgg gtcacttaca ataacccaat gtctatatta ttttgcttta cggagatcac
     3721 tagattcatt ttctgaatca ttgatctgcg ttttttcatt ttcaaggcta attattgtat
     3781 ttttagtcat ttatttttta aactactaat gttaataact ctaaatttga tgttgaatta
     3841 atttgaccga ttttaaagca tatcatcatt tactttttaa tcagagttac atccaaatga
     3901 tagatttcac gttatacctt cacgtataat attatgtatc gtttgtaagc aaatgactaa
     3961 aagtctatta atatatacat ttaattaatt gaaaggattg actacatgat acaagatgcg
     4021 tttgttgcac ttgattttga aacagcaaat ggtaaacgta caagtatttg ttctgtcgga
     4081 atggttaaag tcattgatag tcaaataaca gaaacatttc atactcttgt gaatccgcaa
     4141 gactattttt cacaacaaaa tattaaagtt catggcatac aaccagaaga tgttgaaaat
     4201 gcacctacgt ttgactacgt atttccatat atgatgcaat ttattgcaga tttacctgtt
     4261 gtcgcacata acgcggcatt tgatatgaac gtcttacatc aaagcattca aaatattggt
     4321 ttaccaactc caaatttaac ttacttttgt agttatcaac ttgctaaaag aaccgttgat
     4381 tcgtatcgat acggtttaaa acatatgatg gagttttatc aattagattt tcatggtcat
     4441 catgatgcat tgaatgatgc caaagcatgc gcaatgatta cttttaggct actgaaaaat
     4501 tatgaaaatt taacatatgt aactaatatt tatggtaaaa atcta
//
LOCUS       DAOBBH010000183         4469 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.183, whole genome shotgun sequence.
ACCESSION   DAOBBH010000183 DAOBBH010000000
VERSION     DAOBBH010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.183"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(11..733)
                     /locus_tag="P6L01_002255"
     CDS             complement(11..733)
                     /locus_tag="P6L01_002255"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDP3031301.1"
                     /translation="MKILLIGYGAMNQRVARLAEEKGHEIVGVIENTPKATTPYQQYQ
                     HIADVKDADVAIDFSNPNLLFPLLDEEFHLPLVVATTGEKEKLLNKLDELSQNMPVFF
                     SANMSYGVHALTKILAAAVPLLDDFDIELTEAHHNKKVDAPSGTLEKLYDVIVSLKEN
                     VTPVYDRHELNEKRQPQDIGIHSIRGGTIVGEHEVLFAGTDETIQITHRAQSKDIFAN
                     GAIQAAERLVNKPNGFYTFDNL"
     gene            complement(730..1617)
                     /locus_tag="P6L01_002256"
     CDS             complement(730..1617)
                     /locus_tag="P6L01_002256"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDP3031302.1"
                     /translation="MTHLFEGVGVALTTPFTNNKVNLEALKAHVNFLLENNAQAIIVN
                     GTTAESPTLTTDEKERILKTVIDLVDKRVPVIAGTGTNDTEKSIQASFQAKALGADAI
                     MLITPYYNKTNQRGLVKHFEAITDAVKLPVVLYNVPSRTNMTIEPETVEILSQHPYIV
                     ALKDATNDFEYLEEVKKRIDTNSFALYSGNDDNVVEYYQRGGQGVISVIANVIPKEFQ
                     ALYDAQQSGLDIQDQFKPIGTLLSALSVDINPIPIKALTSYLEFGNYELRLPLVSLED
                     TDTKVLREAYDTFKAGENE"
     gene            complement(1619..2608)
                     /locus_tag="P6L01_002257"
     CDS             complement(1619..2608)
                     /locus_tag="P6L01_002257"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDP3031303.1"
                     /translation="MTKLAVVGATGLVGTKMLETLNRKNIPFDELVLFSSARSAGQEV
                     EFQEKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKAGAIVIDNSSQWRMAE
                     DIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGM
                     KGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDL
                     KVTATCARVPVQDSHSVEIDVTLDKETTAEDIKALFDQDDRVVLVDNPENNEYPMAIN
                     STNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN"
     gene            complement(2672..3877)
                     /locus_tag="P6L01_002258"
     CDS             complement(2672..3877)
                     /locus_tag="P6L01_002258"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDP3031304.1"
                     /translation="MVTRSVLKFGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAM
                     GNTTDQLMTNVSTLTKAPKQQELALLLTTGEQQTVSYLSMVLNDIGVNAKAMTGYQAG
                     IKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINEHQELTTLGRGGSDTTAVA
                     LAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVEL
                     AKNYNIPLYLGKTLSNVKGTWIMSNEEILEKKAVTGVALDKHMMHVTISYPLPDNQLL
                     TQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILETLKNQYEALAYKI
                     NEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAV
                     EKLYDAFNI"
BASE COUNT         1335 a          835 c          718 g         1581 t
ORIGIN      
        1 tttaatatgt ttataagttg tcaaacgtat aaaagccgtt tggtttatta actaagcgtt
       61 ctgctgcttg tatagcacca ttcgcaaaaa tatcttttga ttgtgcacga tgtgtgattt
      121 gaatcgtttc atcagtgcca gcaaatagaa cttcatgttc accgacaatc gtacctccac
      181 gaatagaatg tataccaata tcttgtggct ggcgtttttc atttaattca tgtctatcat
      241 acacaggtgt tacattttct ttcaaagata cgatcacatc atacaatttt tctaacgtac
      301 cacttggtgc atctactttt ttattatgat gtgcctcagt caattcgatg tcgaaatcat
      361 caagtagggg aacagcagct gctaaaattt tagtcagtgc atgaacgcca taactcatgt
      421 tcgcgctgaa aaatacaggc atattttgac tcaattcatc taacttatta agtagttttt
      481 ctttctcgcc agttgttgcc acaactaatg gcaaatgaaa ctcttcatct aataatggga
      541 ataatagatt cggatttgaa aaatctattg caacatcggc atctttaaca tctgcaatat
      601 gttgatattg ttgatatggc gttgttgctt tcggtgtatt ttcaatgacc ccaacgattt
      661 catgtccttt ttcttctgct aatctagcaa cgcgttgatt cattgcacca tagccaatta
      721 gtaatatttt cactcatttt cacccgcttt aaatgtgtca tatgcttcac gaagcacttt
      781 agtatctgta tcttctaggc taaccaatgg taaacgtaat tcatagtttc caaattctaa
      841 ataacttgtt aaagctttaa taggaattgg gttaatatca actgataggg ctgataacag
      901 tgtgccgatt ggtttaaatt gatcttgaat atctaatcca ctttgttgag catcgtataa
      961 cgcttgaaat tctttaggaa tgacattggc aataacagag ataacccctt gaccgccacg
     1021 ttgatagtat tcgacgacgt tgtcatcatt gccactatat aatgcaaatg aatttgtatc
     1081 aattcgcttt ttcacttctt ctaaatactc aaaatcattc gtagcatctt ttaaagcaac
     1141 tatataagga tgttgactta atatttctac agtttctggt tcaattgtca tgttcgttct
     1201 tgaaggaaca ttgtacagca cgactggtaa tttcacagca tctgtaatcg cttcaaagtg
     1261 tttgactaaa ccacgttggt tcgttttgtt gtagtagggc gtaattaaca taattgcatc
     1321 agcccctaag gctttagctt ggaatgaagc ttggattgac ttttcagtat cattagttcc
     1381 agttccagct atgacaggaa cacgtttatc tacaagatca ataactgttt ttagaatgcg
     1441 ttctttttca tctgttgtta aagtagggct ctcagcagta gttccattaa cgatgattgc
     1501 ttgggcatta ttttctagta aaaaattaac gtgtgctttc aaagcttcaa gattaacttt
     1561 gttatttgta aaaggggttg taagtgcaac gccaacaccc tcaaataaat gtgtcatttt
     1621 aattcgctcc ttttaaacgc ataacttgtt ccaatacttg tacagcattt aatgcagcac
     1681 cttttaataa attgtctgat gtacaccata catggaaagt attttctaat gaatcatctc
     1741 tacgtatacg gccaacaaac acttcatctt tattagtgga attgattgcc attggatatt
     1801 cattgttctc tggattgtct actaaaacaa cgcggtcatc ttgatcaaat aacgctttaa
     1861 tatcttctgc tgtcgtttct ttgtcaagcg ttacatcaat ttcaacacta tgactatctt
     1921 gaacaggcac acgtgcgcat gttgctgtta cttttaagtc tggcgcattt aaaatttttc
     1981 tcgtctcatc aatcattttt tgttcttctt ttgtatatcc gttttctaaa aacacatcaa
     2041 tatgcggtaa cacattatta taaattggat gtggatatgc ttctggtgct ttaccattta
     2101 caccttcagc taaatctttc ttacctttca tccctgaacc tgatacagct tgatatgttg
     2161 tatatgccac tcgttttaaa ccataagcat cttgcaatac ttttaatggc acaacagatt
     2221 gaatcgtaga gcagtttgga ttggcaatga tacctcttgt aaatgtaggt tcattgactt
     2281 ccggaacgat taaatcaata tcttctgcca tacgccattg acttgaattg tctataacga
     2341 ttgcaccagc tttttcaaag agtggggcaa agtgttcgct tgtaccgcca ccagcactca
     2401 ttaatacata atcgaaatgt tcactagcac gagcatcagt taattcttgt actgtatatg
     2461 ttttttcttg aaattcaact tcttgccctg cagaacgtgc tgatgaaaat aatactaatt
     2521 catcgaaagg aatattttta cgatttaatg tctccaacat ttttgtgcct actaatcctg
     2581 ttgcacccac aactgctaac tttgtcataa cttgtcactc cattttataa taatttccaa
     2641 tttttagaat attttaacaa tcattttacc attaaatgtt aaatgcgtca tatagttttt
     2701 ctaccgcttg ttgcccatta aaatcatcaa tgacgtatga aatacttatt tcagatgttg
     2761 ttgtttggta gaaaggtata ttattttcaa ttaatgtcaa aaatgctttt gatgccacac
     2821 ctgacatatc acgcatgcct gagccaatta atgaaatttt gacataatgc tcattgattt
     2881 tataagctaa tgcttcatat tgattcttta atgtttcaag aatcatagaa atttgatgaa
     2941 aatcactatc tttaatcgtg aaggatagtt gtagcccatc caagttgacg atttgtgaaa
     3001 tcatatcaac atttacagca ccttcttcaa gttccgtaaa tagttgggta agtagctgat
     3061 tgtcaggtag gggataacta attgttacat gcatcatatg tttatccaaa gccacaccag
     3121 taactgcttt tttctctaat atttcttcat ttgacataat ccatgttcct ttcacgttcg
     3181 ataaagtttt tcctaaatat aaagggatat tatagttttt agctaattca acacttcttg
     3241 tttcaagtac accagcacct aaagcgctca tttccatcat ttcttcatat gagacgatgt
     3301 ctagtcgttt agcctttggt aaaagtcttg ggtcagtggc atacacacca tcaacgtcgg
     3361 tataaatttc acaaggtatt tgattactaa cagcaagtgc cacagcggtc gtatcagaac
     3421 cgcctctgcc taaagttgtt aattcctgat gttcattgat gccttgaaat ccagcaacta
     3481 ctaaaatatc gttttcttga aaggcttgtt caaatgtttg aggattaatt tgagcaattt
     3541 tactttttaa atgatggcca atggttttaa tacccgcttg atagccagtc attgctttgg
     3601 cattcacacc gatatcattt aataccattg ataaataaga tacagtttgt tgctctccgg
     3661 ttgtcaataa taatgccagt tcttgttgtt ttggtgcttt agtcaaggtc gatacattcg
     3721 tcattaattg atctgttgtg ttacccatag cacttacaac gacaattaat tgttcatctt
     3781 gattgactcg ctcctttaac atttcagcga tcctttttat ttttgtaaaa tcactgacgg
     3841 atgatccgcc aaatttcaac acacttcttg ttaccatata atcctcctaa ttgttaggaa
     3901 gggcacacaa taataaaaaa agaacaagtt cggaatgcga acttgctctt aatctatata
     3961 caagtgatgc actccattat ttttaaataa tgacaaactc tcagctctta accaaaaagt
     4021 ccaacaaatt ataactgcta ttataatcgc ttcggcatcg caccctttca aattcagctg
     4081 ttagcagaca gtaatctaaa ctttactcat gattgatgcg cctcatcaaa atattattaa
     4141 attggtatgt gttcattata catatgacaa atatgaatgt aaaccgataa tttagatttt
     4201 ttggaataac ctgaaaattc aagttatagc gttgcttata ttttaaaagg tggtgataat
     4261 gagacttttt gaaaaataaa attcaaaata cttatagcat actcaatatg cacattaaat
     4321 aaatgtactc ttttaatgcg gtgataactg tatttgtaat ttagagaagg ggtgttcact
     4381 gtgctgatgt gctaaaaaat aaaataaaaa ggacacgagg ctgggatatt aagttcttag
     4441 gcaatgtaaa aagaggctga gacggcacc
//
LOCUS       DAOBBH010000184         4442 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.184, whole genome shotgun sequence.
ACCESSION   DAOBBH010000184 DAOBBH010000000
VERSION     DAOBBH010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.184"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            90..1271
                     /locus_tag="P6L01_002259"
     CDS             90..1271
                     /locus_tag="P6L01_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDP3031305.1"
                     /translation="MNIPKSVWWLVIGMALNITGSSFLWPLNTIYMKQELGKSLTVAG
                     LVLMINSFGMVIGNLLGGSLFDKLGGYKTILIGTFTCLCSTTLLNFFHGWPWYAVWLV
                     MLGFGGGMIIPAIYAMAGAVWPNGGRQTFNAIYLAQNIGVAVGAAMGGFVAEFSFNYI
                     FLANLIMYVVFALVAVTQFNIEINAKVKYPTHLDITGKKNKARFISLVLICAMFAICW
                     VAYIQWESTIASFTQSINISMAQYSVLWTINGIMILVAQPLIKPVLYLLKGNLKKQMF
                     VGIIIFMLSFFVTSFAENFTIFVVGMIILTFGEMFVWPAVPTIANQLAPDGKQGQYQG
                     FVNSAATVGKAFGPFLGGVLVDAFNMRMMFIGMMLLLVFALILLMVFKENNTQPKKID
                     A"
     gene            1559..3976
                     /locus_tag="P6L01_002260"
     CDS             1559..3976
                     /locus_tag="P6L01_002260"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDP3031306.1"
                     /translation="MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPS
                     GAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTK
                     KNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
                     ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDM
                     QRNWIGRSEGAKVSFDVDNTEGKVEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYK
                     EKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYAINPLSGEKVQIWIADYVLSTYGTG
                     AIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAI
                     TKAIQLLEQKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLL
                     PETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYIDPK
                     NENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLGIVPTKEPFQKLFN
                     QGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGS
                     RRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLM
                     VFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTYDEALL
                     VDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQK
                     LVNIVAK"
     gene            3998..4309
                     /locus_tag="P6L01_002261"
     CDS             3998..4309
                     /locus_tag="P6L01_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDP3031307.1"
                     /translation="MKSITTDELKNKLFESKPVQIVDVRTDEETAMGYIPNAKLIPMD
                     TIPDNLNSFNKNEIYYIVCAGGVRSAKVVEYLEANGIDAVNVEGGMHAWGDEGLEIKS
                     I"
BASE COUNT         1522 a          589 c          911 g         1420 t
ORIGIN      
        1 aaaaaagagt tctaagtaca gattttaaca tattttaatg ttatagtgtt tattatagtt
       61 tgacaaaaaa gagagaggaa ctatgaaata tgaatatacc taaatcagtc tggtggctag
      121 taattggcat ggcgttaaat attactggtt ccagtttttt gtggccttta aatacaattt
      181 atatgaaaca agaacttgga aaaagtttaa ctgttgctgg tttagtgcta atgataaatt
      241 catttggcat ggttattgga aacttattag gtggttcact atttgataaa ttaggtggat
      301 acaagacgat tttaattgga actttcactt gtctttgtag tacaacgcta cttaatttct
      361 ttcacgggtg gccttggtat gctgtatggc ttgtaatgtt aggctttggt ggcggaatga
      421 ttattcctgc gatatacgct atggctggag cagtgtggcc gaatggcgga agacaaacgt
      481 ttaatgcgat atacttagcg caaaatattg gtgtggctgt cggtgctgca atgggcggct
      541 ttgtcgcaga atttagcttt aactatatct ttttagccaa tcttattatg tatgttgtgt
      601 ttgcgcttgt cgcggtaacg caatttaata ttgaaattaa tgcgaaagtt aaatatccaa
      661 ctcatttaga tattactggt aaaaagaata aagcaagatt tatttcatta gtactaattt
      721 gtgcaatgtt tgcaatttgt tgggttgcgt atattcaatg ggagtctaca atcgcatcat
      781 ttacacaatc tattaatatt tcaatggcac aatatagtgt tttatggaca attaacggaa
      841 taatgatttt agtagcacaa ccattaatta aaccggttct ctatctgtta aaaggaaact
      901 taaagaagca aatgtttgtc ggcatcatca tttttatgtt gtcgttcttt gtcacgagtt
      961 ttgccgaaaa ctttacaata tttgttgtcg gtatgattat tttaactttt ggagaaatgt
     1021 ttgtatggcc agcagttcca actatagcca atcagttagc gccagatggt aagcaaggac
     1081 agtaccaagg ttttgtgaat tcagctgcta cagtaggaaa agcatttggt ccatttcttg
     1141 gtggtgtatt agttgatgcg tttaatatgc gcatgatgtt tatcggtatg atgctactac
     1201 ttgtatttgc attaatatta ttaatggttt tcaaggagaa taatacgcaa cctaaaaaaa
     1261 tagatgcata atgagtaaat agaattaacg ttatagactt gaaataaatg tcgttataac
     1321 ataatattaa tttgtataat ttaatttcgt ttggagcttt tctacagaaa gctagtgatg
     1381 ctgagagcta gtgttaagga ctaaatgtaa atcgtattaa ttttaaattg aatgaatgac
     1441 atctcttact attaaaatga gtgcacaatt tttgtgaaat agggtggtaa cgcggcaaat
     1501 gtcgtcccta tgtaaataga atagttagag gtgtcttttt tattgaatag gaggaaatgt
     1561 gttgaattac aaccacaatc aaattgaaaa gaaatggcaa gactattggg acgaaaataa
     1621 aacatttaaa acaaatgata acttaggtca aaagaaattt tatgctttag acatgtttcc
     1681 atatccatca ggtgctggtt tacatgttgg acatcctgag ggctatacag caacagatat
     1741 catttcaaga tataaaagga tgcaaggtta taatgtatta catccgatgg ggtgggatgc
     1801 attcggatta ccagcagagc aatatgcttt agacactggc aacgacccac gtgaatttac
     1861 aaagaaaaat atccaaactt ttaaacgaca aattaaagaa ttagggttca gttatgattg
     1921 ggatcgtgaa gttaatacaa cagatccaga atactataaa tggacacagt ggattttcat
     1981 acagttatat aacaaaggtt tagcatacgt tgatgaagtt gcagttaact ggtgtccagc
     2041 attaggcact gttttatcta acgaagaagt gattgatggt gtctctgaac gtggtggaca
     2101 tccagtttat cgtaagccga tgaaacaatg ggtacttaaa atcacagaat atgcagatca
     2161 attattagca gatttagatg atttagattg gcctgagtct ttaaaagata tgcagcgcaa
     2221 ttggattgga cgttctgaag gggccaaagt ttcatttgat gtagataata cggaaggaaa
     2281 agtagaagta tttacgacta gaccagacac tatctatggt gcatcattct tagtcttaag
     2341 tcctgaacat gcattagtta attcaattac aactgatgaa tataaagaaa aagtaaaagc
     2401 ttatcaaaca gaagcttcta aaaagtcaga tttagaacgt acagatttag caaaagataa
     2461 atcaggtgtg tttactggtg catatgcaat taatccttta tctggtgaaa aagtacaaat
     2521 ttggattgct gattatgtat tatcaacata tggtactgga gcaattatgg cagtaccagc
     2581 gcatgatgac agagattatg aatttgctaa aaagtttgat ttgccaatca ttgaagtcat
     2641 cgaaggtgga aatgttgaag aagcagcata cactggtgaa ggtaaacata ttaattctgg
     2701 tgaacttgat ggtttagaaa atgaagcggc aattactaaa gctattcaat tattagagca
     2761 aaaaggtgct ggcgaaaaga aagttaatta caaattaaga gattggttat tcagtcgtca
     2821 gcgttattgg ggcgaaccaa ttcctgtcat tcattgggaa gatggaacaa tgacaactgt
     2881 tcctgaagaa gagctaccat tgttgttacc tgaaacagat gaaatcaagc catcagggac
     2941 tggtgagtct ccactagcta atattgattc atttgtaaat gttgtagatg aaaaaacagg
     3001 tatgaaagga cgtcgtgaaa caaatacaat gccacaatgg gcaggtagtt gttggtatta
     3061 tttacgttac atcgatccta aaaatgaaaa tatgttagca gatcctgaaa aattaaaaca
     3121 ttggttacct gttgatttat atatcggtgg agtagaacat gcggttcttc acttattata
     3181 tgcaagattt tggcataaag tcctttatga tttgggtatc gtacctacta aagaaccttt
     3241 ccaaaaatta tttaaccaag gtatgatttt aggagaaggt aatgagaaga tgagtaaatc
     3301 taaaggaaat gtaatcaatc ctgatgatat agtacagtct catggtgcag atactttacg
     3361 tctttacgaa atgtttatgg gacctttaga tgctgcaatt gcatggagtg aaaaaggatt
     3421 agatgggtct cgtcgattct tagatcgcgt atggcgttta atggtaaatg aagatggaac
     3481 attgagttca aaaattgtaa ctacaaataa taaatcttta gataaagttt ataaccaaac
     3541 tgttaaaaag gtaacagaag actttgaaac attaggattt aatactgcta ttagtcaatt
     3601 aatggtattt attaatgagt gttataaagt tgatgaagtt tataaacctt acattgaagg
     3661 cttcgttaaa atgttagcac ctattgcacc acatatcggt gaagaattat ggtcaaaatt
     3721 aggacatgaa gagtctatta cgtaccaacc ttggccaact tatgacgaag cactacttgt
     3781 agatgatgaa gtagaaatcg ttgttcaagt aaatggtaaa ttgagagcta aaattaaaat
     3841 tgctaaagat acatcaaaag aagaaatgca agaaattgcc ttatctaatg acaatgttaa
     3901 agcgagtatt gaaggtaaag acatcatgaa agtcatcgct gttcctcaaa aattagtcaa
     3961 tattgtagct aaataatgtt ttaaggagga ctttgaaatg aagtcaatta ctacagatga
     4021 attaaaaaac aaactttttg aatctaaacc agttcaaatt gttgatgttc gtactgacga
     4081 agaaacagca atgggatata ttcctaatgc aaagttgatt ccaatggata ccattccgga
     4141 taatttaaat tcatttaata aaaatgaaat atattatatt gtatgtgctg gtggagttcg
     4201 aagcgctaaa gttgtagaat atttagaggc aaatggcatt gatgccgtaa atgtcgaagg
     4261 cggcatgcac gcatggggcg atgaaggttt ggaaataaaa agtatttaaa gtagtgacat
     4321 aatttaaaat aatattacat ttgtaatgac accaagtaac gtttcggttg cttggtgttt
     4381 tttggtatga attactttct gttacaaaac aatctaaagc gttcttgtta tgttttatta
     4441 ag
//
LOCUS       DAOBBH010000185         4404 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.185, whole genome shotgun sequence.
ACCESSION   DAOBBH010000185 DAOBBH010000000
VERSION     DAOBBH010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.185"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..711
                     /gene="whiA"
                     /locus_tag="P6L01_002262"
     CDS             <1..711
                     /gene="whiA"
                     /locus_tag="P6L01_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDP3031308.1"
                     /translation="LKKNNIYICRTKMKAKEILDELGILKDGIFTHEIDHSMIQDDEM
                     RRSYLRGAFLAGGSVNNPETSSYHLEIFSQNESHAEGLTKLMNSYELNAKHLERKKGS
                     ITYLKEAEKISDFLSLIGGYQALLKFEDVRIVRDMRNSVNRLVNCETANLNKTVSAAM
                     KQVESIKLIDKEIGIENLPDRLREIARIRVEHQEISLKELGEMVSTGPISKSGVNHRL
                     RKLNDLADKIRNGEQIEL"
     gene            1275..1862
                     /gene="clpP"
                     /locus_tag="P6L01_002263"
     CDS             1275..1862
                     /gene="clpP"
                     /locus_tag="P6L01_002263"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDP3031309.1"
                     /translation="MNLIPTVIETTNRGERAYDIYSRLLKDRIIMLGSQIDDNVANSI
                     VSQLLFLQAQDSEKDIYLYINSPGGSVTAGFAIYDTIQHIKPDVQTICIGMAASMGSF
                     LLAAGAKGKRFALPNAEVMIHQPLGGAQGQATEIEIAANHILKTREKLNRILSERTGQ
                     SIEKIQKDTDRDNFLTAEEAKEYGLIDEVMVPETK"
     gene            complement(2105..3007)
                     /locus_tag="P6L01_002264"
     CDS             complement(2105..3007)
                     /locus_tag="P6L01_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDP3031310.1"
                     /translation="MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNDKKIS
                     YVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSR
                     NKAPKVLFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVV
                     IGRFGMILSNEGGALQTMKLPYKYYIGGKLGSGQQWYSWIHINDLIQAILFLINNESA
                     SGPFNLTAPIPERQNLFGYTLARAMHKPHETWAPSLAMRLILGQMSTVVLDTQKVLPN
                     KIQALGFQFKYSNLKMALEDLIKE"
     gene            3710..4339
                     /locus_tag="P6L01_002265"
     CDS             3710..4339
                     /locus_tag="P6L01_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4887 domain-containing protein"
                     /protein_id="HDP3031311.1"
                     /translation="MSNQNYDYNKNEDGSKKKMSTTAKVVSIATVLLLLGGLVFAIFA
                     YVDHSNKAKERMLNEQKQEQKEKRQKENAEKERKKKQQEEKEQNELDSQANQYQQLPQ
                     QNQYQYVPPQQQAPTKQRPAKEENDDKASKDESKDKDDNASQDKSDDNQKKTDDNKQP
                     AQPKPQPQQPTPKPNNNQQNNQSNQQAKPQAPQQNSQSTTNKQNNANDK"
BASE COUNT         1676 a          641 c          759 g         1328 t
ORIGIN      
        1 cttaaaaaaa ataatattta tatttgtcgt acaaagatga aagcgaaaga aattcttgat
       61 gaattaggaa ttttaaaaga cggcattttt acgcatgaaa ttgatcattc aatgattcaa
      121 gatgatgaaa tgagacgcag ttacttgaga ggagcttttc tggcaggtgg ctcagtgaat
      181 aaccctgaaa catcttcgta ccatttggaa attttttctc aaaatgagag tcatgcagaa
      241 ggcttaacga aactaatgaa tagttatgag ttgaatgcca aacatttaga gcgaaaaaaa
      301 ggaagtatta cgtatttaaa agaagcggaa aagatttcgg attttcttag tttgataggt
      361 ggctatcaag cgttattaaa atttgaagac gtacgtattg taagagatat gcgtaattct
      421 gttaaccgac tcgttaattg tgaaacggca aatctaaata aaacagttag tgctgcgatg
      481 aaacaagttg agagcattaa attgattgat aaagaaattg gtattgaaaa tttaccagac
      541 aggttgagag agattgctag aattcgagta gaacatcaag aaatttcgtt gaaagagctt
      601 ggagaaatgg tatcaactgg tccaatttca aaatcaggtg taaatcaccg attaagaaaa
      661 cttaatgatt tagccgataa gattagaaat ggtgaacaaa tagaattata agtaagaagg
      721 tgtttttggt agtgaattat caaaaatacc ttttttatga ttaaaaagtg tttaatgaat
      781 aatagtcgac taactattaa attgagctgg aaagtttaat gaaggaaatt caataaccaa
      841 gcgttaccta attttctcat taagtagcat catatgtatc aatttggtca taagaaaata
      901 tgaatgtgat aattaattga gtcactgatc atagacaaaa acaaaatcag cataaataag
      961 aatcccgact ttgcgggatt ctctgtattg aaaagtattg tattttatta aaacagcctc
     1021 cttgaaggga attgaacccc tatcttaaga accggaatct tacgtgttat ccattacacc
     1081 acaaggagca aatatgtaat catatctctg tactatgaga agtaaaataa gtacaatcta
     1141 aaatagatat tatcagttta caaaggaaag agaaaagcgt caaacaatgt aactatttaa
     1201 agtcaaagtg tttgaccaaa tttgacttaa tatgtaaaat aatgagtaac agttattaca
     1261 aggaggaaat atagatgaat ttaattccta cagttattga aacaacaaac cgcggtgaac
     1321 gtgcatatga tatatactca cgtttattaa aagaccgtat tattatgtta ggttcacaaa
     1381 ttgatgacaa cgtagcaaat tcaatcgtat cacagttatt attcttacaa gcgcaagact
     1441 cagagaaaga tatttattta tacattaatt caccaggtgg aagtgtaaca gctggttttg
     1501 cgatttatga tacaattcaa cacattaaac ctgatgttca aacaatttgt atcggtatgg
     1561 ctgcatcaat gggatcattc ttattagcag ctggtgcaaa aggtaaacgt ttcgcgttac
     1621 caaatgcaga agtaatgatt caccaaccat taggtggtgc tcaaggacaa gcaactgaaa
     1681 tcgaaattgc tgcaaatcac attttaaaaa cacgtgaaaa attaaaccgc attttatcag
     1741 agcgtactgg tcaaagtatt gaaaaaatac aaaaagacac agatcgtgat aacttcttaa
     1801 ctgcagaaga agctaaagaa tatggcttaa ttgatgaagt gatggtacct gaaacaaaat
     1861 aattcaaagt aaagagtaga ctaagcagtc tgctcttttt gtatgagtaa accgaggtgt
     1921 caataaattg tttactatac tttgagcgga aatatgattg aatgaagcta gttgaaccgt
     1981 aactatatga aatgttccct tcaaagtaga cattgaaagg aacatttcaa tcctttgttt
     2041 gtaagtcgct ctagacatta catttagtac atatgttgtt tctaatgctc attaatggta
     2101 ttgattattc tttgattaaa tcttcaagtg ccatttttaa attactatat ttaaattgga
     2161 atcccaatgc ttgaatttta ttaggtaata ctttttgagt atccaatact actgttgaca
     2221 tttgaccaag tatgagacgc attgcaagac ttggtgccca agtttcatgg ggcttatgca
     2281 tagctcttgc taaagtgtag ccaaataaat tttgacgctc aggtataggt gcagttaaat
     2341 taaacggacc actagctgac tcgttattta ttaaaaataa aatagcttga attaaatcat
     2401 tgatatgaat ccatgaatac cattgttgac cagaacctaa tttaccacca atgtaatatt
     2461 tgtatggtag tttcattgtt tgtaacgcac cgccttcatt cgataaaatc ataccgaaac
     2521 gaccgatgac aactcgcgta cctaattgtt caaattgttg tgcgaaacgt tcccattgat
     2581 acacaatatc tgataagaaa tcaaatggta aagttttata aacttctgtg taactcataa
     2641 ataaatcagg aggatagtaa ccagtggcgc tagcattaaa taaaacttta ggtgctttat
     2701 tacgtgattt aaacaattca tataaagctt gcgtagattg aattctactt agcattagcg
     2761 tttgtttata ttccggtgtc catcgtttat tcagtgtagc acctgctaag ttgatgacca
     2821 catcgatatt ttgaggaact ttgtgttccc acccagattt agcccagttg acatatgaaa
     2881 ttttcttatc atttgaaatt tggtcgtgtc gcgttaatat cgtgatatgt gaatctgatt
     2941 ttttaatttc attaactaat tgagatccaa ccataccagt cccaccagta attaagtatt
     3001 gtttcattat cattcacccc atgtaatttt gtatttagtt actatttaat aatcattatt
     3061 gttgttcaaa ggttatacat tatttagaca ataatatgtc aataactttt tttgaattgt
     3121 attttaataa aaatgatata agttataata aaggtgtacc ttcgataacg aataaacatc
     3181 tcttaaaagt atgtgtaaaa cgctgcatga tacaaacgaa ggtaaaaatt tgactccctt
     3241 tagtagtgga cccgtacgtt aatcgtgcgg gtcgttttta tttatttctt attcccatta
     3301 tacatcaatt taaagcatta attttttaaa caaatttaag aatacatagt aatataacaa
     3361 tctaaacata aaaactttta acacaacact taaaccaatg ctttaatttt caatacgtag
     3421 ctataatttg ttgtaaaatc aaaaaggtta aaatgttaat tttcaaaaaa aggctcaaaa
     3481 tatgtttgat ttagttatta aatgttaaga tatataagac tactatttct ttgtaaaaat
     3541 gaatccgatt tacgagtgag taatagtgaa ggcagtttta agttgaagaa ggcaaaaaga
     3601 gtaaatgttt attaatattt gtagaaacta ggtaagcaaa ttagttgtga aaatgttaat
     3661 ggttgcgtga taatttctat atttaaatta gtttgaagtg agggagagta tgtcgaatca
     3721 aaattacgac tacaataaaa atgaagatgg aagtaagaag aaaatgagta caacagcgaa
     3781 agtagttagc attgcgacgg tattgctatt actcggagga ttagtatttg caatttttgc
     3841 atatgtagat cattcgaata aagctaaaga acgtatgttg aacgaacaaa agcaggaaca
     3901 aaaagaaaag cgtcaaaaag aaaatgcaga aaaagagaga aagaaaaagc aacaagagga
     3961 aaaagagcag aatgagctag attcacaagc aaaccaatat cagcaattgc cacagcagaa
     4021 tcaatatcaa tatgtgccac ctcagcaaca agcacctaca aagcaacgtc ctgctaaaga
     4081 agagaatgat gataaagcat caaaggatga gtcgaaagat aaggatgaca atgcatctca
     4141 agataaatca gatgataatc agaagaaaac tgatgataat aaacaaccag ctcagcctaa
     4201 accacagccg caacaaccaa caccaaagcc aaataataat caacaaaata atcaatcaaa
     4261 tcagcaagca aaaccacaag caccacaaca aaatagccaa tcaacaacaa ataaacaaaa
     4321 taatgctaat gataagtagt atttagtcaa acaaaaatga accagtatga cagacaacac
     4381 aattaattag gttgtctcga atat
//
LOCUS       DAOBBH010000186         4391 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.186, whole genome shotgun sequence.
ACCESSION   DAOBBH010000186 DAOBBH010000000
VERSION     DAOBBH010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.186"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(105..749)
                     /gene="trmB"
                     /locus_tag="P6L01_002266"
     CDS             complement(105..749)
                     /gene="trmB"
                     /locus_tag="P6L01_002266"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDP3031312.1"
                     /translation="MRVRYKPWAEDYLKDHPELVDMEGQHAGKMTEWFDKTQPIHIEI
                     GSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMGLTNLKIICNDAIELNEY
                     FKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
                     SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK"
     gene            complement(764..1555)
                     /locus_tag="P6L01_002267"
     CDS             complement(764..1555)
                     /locus_tag="P6L01_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDP3031313.1"
                     /translation="MEQFYQLGWTLDSAGGASGEAYMAEQDGQKLFLKRNSNPFIAAL
                     SAEGIVPKLVWTKRIETGEVVTAQHWKNGRELSSNEMKQTRVAHLLKKIHNSRPLLSM
                     LKRMEMEPITPEIMLNKINASLSREVLTHHIVRKSLTYLEEHIPSLDSRFFTVVHGDV
                     NHNNWLLSDRDELFLVDWEGAMIADPAIDIGMLLYNYVPQQQWSEWLETYGVQESLNL
                     NKRMKWYTVIQSIGLVQWYEEQKRYKDMNTWLKFLNEVMNSNMFI"
     gene            complement(2105..2953)
                     /gene="dat"
                     /locus_tag="P6L01_002268"
     CDS             complement(2105..2953)
                     /gene="dat"
                     /locus_tag="P6L01_002268"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HDP3031314.1"
                     /translation="MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFT
                     VTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHS
                     FPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYA
                     VKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYN
                     IPFKEETFTVDFLKNADEVIVSSTSAEVTPVIKLDGEPINDGKVGPITRQLQEGFEKY
                     IESHSI"
     gene            complement(2957..4366)
                     /gene="sapep"
                     /locus_tag="P6L01_002269"
     CDS             complement(2957..4366)
                     /gene="sapep"
                     /locus_tag="P6L01_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+)-dependent dipeptidase Sapep"
                     /protein_id="HDP3031315.1"
                     /translation="MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRK
                     ALDYMYEIAHRDGFTTHDVDHIAGRIEAGKGNDVLGILCHVDVVPAGDGWDSNPFEPV
                     VTEDAIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYF
                     KTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLTEDQDEPDYELITFKSGERYNMV
                     PDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIG
                     VNAGLYLLKFLASLNLDNNAQAFVAFSNRYLFNSDFGEKMGMKFHTDVMGDVTTNIGV
                     ITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPF
                     VQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQ
                     LFNATSIYLEAIYSLCVEE"
BASE COUNT         1371 a          867 c          568 g         1585 t
ORIGIN      
        1 acttttttcc atcagtccga tttattatag aacctttatc tatagaacca ttatagtacg
       61 aaaaaacact tatgattgta tgtgtacata ccctcataag tgttttattt ttgtgaatgg
      121 aatttcgctt ccatacgata aatacgtgac cctttatccg aaaatttctt ttcatattct
      181 gttaaaatat tactgccatc gtcttcttga tgtaaattta gatttatttt tgtaaaatac
      241 attccaaatt gagacatact ttctaaactg taggcaaata gtcctctgtt atcagtttta
      301 aaatgtaaat ctccttcatc atttaagatt tgttgataca acgctaaaaa cgtatgatac
      361 gttaaacgtc gttttgcatg acgatttttt ggccatggat ctgaaaagtt caaataaata
      421 cgcgaaactt cgccgtcttt aaaatattca tttaattcaa tggcgtcatt acaaataatc
      481 tttaaatttg ttaaacccat ctctttaact ttatccaata ctttataaac gatacttttc
      541 tcacgttcca ttgaaatata gttaatatga ggattttgag cagctaatgt tgtaataaac
      601 tgccccatac ccgaaccaat ttcaatgtgt atcggttgcg ttttatcaaa ccattcagtc
      661 attttccctg catgttgacc ttccatgtca accaattcag gatgatcttt taaataatct
      721 tcagcccatg gtttgtatcg aactctcata ttttattctc ctcttaaata aacatgttac
      781 tattcataac ttcatttagg aatttaagcc aagtgttcat atccttatat cttttttgct
      841 cttcatacca ttgaacaaga cctatagatt gaattaccgt ataccatttc atacgtttat
      901 ttaaattcaa gctctcttga acaccatatg tttcaagcca ttcagaccat tgttgttgtg
      961 gaacatagtt gtaaagcagc attccgatat caattgccgg gtctgcaatc attgcacctt
     1021 cccaatcaac taaaaatagt tcatctcgat cggataataa ccaattatta tgattcacat
     1081 caccatgtac aacagtgaaa aaacgcgaat ctaaactcgg tatatgctct tctaaatagg
     1141 ttaatgattt tctcacaata tgatgtgtta aaacttctct tgataaagag gcattaattt
     1201 tattaagcat aatctcagga gtaataggtt ccatttccat acgctttaac atacttaata
     1261 aaggtctaga attgtgtatc ttctttaata aatgtgcaac tcttgtttgc ttcatttcgt
     1321 ttgaagatag ttcacgccca tttttccaat gttgtgctgt aacaacctcg cctgtttcta
     1381 tgcgtttcgt ccatactaat ttgggcacaa taccttctgc tgataatgcc gcaataaatg
     1441 gatttgaatt tcgttttaaa aacaactttt gtccatcttg ttcagccata tatgcttcac
     1501 cagatgcacc acctgctgaa tcaagtgtcc accctaattg ataaaactgc tccaactcgt
     1561 ccacctcact ttcaattaga aaatggctct agaaataggt ttttcaagag ccatatattc
     1621 taatttataa caccatactg gtacaaatat tatgtccaga taattattgt aaatcctcaa
     1681 ccaatgccta cattacacga ctaattttaa atcgtaatgt ctgtcattga caccatacat
     1741 tctatagtca cttacttgac atataatgtt accgtgtcta aaactacatg tttttgaatc
     1801 tctgtaggcg ataaactcta gttttcaaaa taattgctat cccattttca tggttagcat
     1861 aaatttatga actgtaacat ttacgtactt agtaaaatat gatgcacatc atatttgtaa
     1921 ctcatagaaa attttataat ttttatcatt atatttcaac tgaaaatgag aaacaaaatg
     1981 gcacttttta ctaatatgta ttttctaaac aacactttta agcttcgttt taaattataa
     2041 cataattcac ttacgaaagt tgataaattt aagtaattta atctaaaaat atgatgaaag
     2101 aattttaaat actgtgtgac tctatatact tttcaaatcc ttcttgtagt tgacgtgtaa
     2161 ttgggccaac tttaccatca ttaattggtt caccatctaa tttaataaca ggtgtaacct
     2221 cagctgaagt acttgaaaca ataacttcat ctgcgttttt caagaaatct acagtaaacg
     2281 tttcttcttt aaatgggatg ttatagtctt cggcaatttt tttaattaca attcgtgtaa
     2341 taccattaag aatatagttg ttaatcggat gtgtataaat cacaccgtct ttaattgcat
     2401 aagcattact tgaagatcct tcagttacag tttcacctcg atgttgaatt gcttcaactg
     2461 cattatattt cacagcatat tcttttgcta atacatttcc taataagttc aagcttttaa
     2521 tgtcgcaacg taaccatcgg atatcttcaa cggtaacacc attcacacca ttttctaaat
     2581 gatcataagg acgatcataa ctctttgtat aagcaacaat tgctggttct acttcaggtg
     2641 tcgggaagct atgattcctt tcagctacac cacgcgttgc ttgaatataa attgccccag
     2701 tttcaatttg attcatatca actaatttac gagatagttc aattaattct tctacagaat
     2761 aatttaaatc taaaccaatc tcattggcac tacgtaaaaa tctttcataa tgttctgtta
     2821 ctgtaaataa cttaccatta tatactcgaa tgtattcata aataccatcg ccaaatacgt
     2881 atcctctgtc gttgtatgaa acctttgctt cacttggact tacaaactca ccatttaaaa
     2941 aaattttttc catatattat tcctccacgc ataatgaata aattgcttct aagtaaatac
     3001 tagttgcgtt aaataactgt tttttagtga tatattcatt tttctgatgc attaaatctt
     3061 cagaatcact aaacattgcg ccaaatgcta cacccttgtc taagtttctc gcataagtac
     3121 cgccacctat agtataaggt tcagtcatat catttgtttg atttctatat gcagtaacta
     3181 acttttgtac aaaaggatca tttttatcaa cataatgtgg tggttggact ttacctaatt
     3241 tcacttcaaa gccatattgt tgaatctcat ttgcaaaacg atccatagct ttttcaaatt
     3301 caaatccttc tgggtagcgt aagttgatac cgaaaagacc tgcgttttca ttatcatatg
     3361 taataacacc aatgttagtt gtcacgtcac ccatgacatc tgtatggaat ttcattccca
     3421 tcttttcacc aaaatctgaa ttaaataagt agcgattact aaatgctaca aacgcttgtg
     3481 cattattatc aagatttaat gatgctaaga attttagtaa gtaaagaccc gcattcacac
     3541 cgatagatgg atccatacca tgaaccgctt taccttcaac tgttaaaact agaatgccac
     3601 tatcaacagt actatcacct tgtaaatgat tttgttctaa aaagtactca aagtcttgaa
     3661 taacatctgt catattttct ttaacaagca ctcttgcttc tgcatgatca ggtaccatgt
     3721 tgtaacgttc accagattta aaagttatta attcataatc aggttcatct tgatcttcag
     3781 taagtttatt ttgaactaaa tcaaatgttg taatgccttt ttcaccatga atacatggaa
     3841 attctgcatc tggtgcaaaa cctaatgttg gcatttcttc tgttttaaaa tagcgatccg
     3901 tacatttcca atcagattct tcatccgtac caataatcat atgaatacgt ttcttccaat
     3961 ccacattcat atcttctaat atcttaattg cataataagc agcaattgtt ggacctttgt
     4021 catcaagtgt acctctagct atgatagcat cttctgttac aaccggctcg aacggattac
     4081 tatcccatcc atcaccagca ggaacaacgt caacatgaca taagatacct aatacgtcat
     4141 ttcctttacc tgcctcaatt cttcctgcaa tatgatccac atcatgtgtt gtaaatccat
     4201 ctctatgtgc aatttcatac atgtagtcta atgccttacg aggacctgga ccaactggtg
     4261 cgtcttctga tgcttttgca tcatctctca cactttcaat tgctaataat ccttttaagt
     4321 cattaatgat ttgatcttcg tattgttgaa ctttttcttt ccacattcga aatcgacttc
     4381 cttttttcta t
//
LOCUS       DAOBBH010000187         4310 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.187, whole genome shotgun sequence.
ACCESSION   DAOBBH010000187 DAOBBH010000000
VERSION     DAOBBH010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.187"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(13..291)
                     /gene="qoxD"
                     /locus_tag="P6L01_002270"
     CDS             complement(13..291)
                     /gene="qoxD"
                     /locus_tag="P6L01_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit IV"
                     /protein_id="HDP3031316.1"
                     /translation="MKHTVGFIASIVLTLLAVYVTLYTSLTFHAKLTIIFGFAFVQAG
                     LQLLMFMHLTEGKDGRLQTFKVIFALVITLCFVVGTYWVMQGGHSSHL"
     gene            complement(300..905)
                     /gene="qoxC"
                     /locus_tag="P6L01_002271"
     CDS             complement(300..905)
                     /gene="qoxC"
                     /locus_tag="P6L01_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit III"
                     /protein_id="HDP3031317.1"
                     /translation="MSHDTNTIDSRTHEGELNKLGFWIFITAEFALFGTLFATLLTLQ
                     HGGDYAGKMTTELFELPLVLIMTFALLFSSYTCGIAIYYMRQEKQKLMMFWMIITLLL
                     GLVFVGFEIYEFAHYASEGVNPTIGSYWSSFFILLGTHGCHVSLGIVWAICLLIQIQR
                     RGLDKYNAPKLFIVSLYWHFLDVVWVFIFTAVYMIGMVYSG"
     gene            complement(895..2883)
                     /gene="qoxB"
                     /locus_tag="P6L01_002272"
     CDS             complement(895..2883)
                     /gene="qoxB"
                     /locus_tag="P6L01_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit I"
                     /protein_id="HDP3031318.1"
                     /translation="MNFPWDQLLVKGNWMITMAQIGAPFLVIGLIAVITYFKLWKYLY
                     KEWFTSVDHKKIGIMYLICAVLMFVRGGIDALLIRAQLTVPDNKFLESNHYNEIFSTH
                     GVIMIIFMAMPFIFGLWNIVVPLQIGARDVAFPVLNNVSFWLFFAGMILFNLSFIIGG
                     SPAAGWTNYAPLAGEFSPGPGVNYYLIAIQISGLGTLATGINFFVTILRCKTPTMKFM
                     QMPMFTVTTFITTLIVILAFPPLTVALALMTTDRIFDTAFFTVAHGGMPMLWANFFWV
                     WGHPEVYIVILPAFGIYSEIIPTFARKRLFGHQSMVWATAGIAFLSFLVWVHHFFTMG
                     NGALINSFFSISTMLIGIPTGVKLFNWLLTLYKGRITFESPMLFSLAFIPNFLLGGVT
                     GVMLAMASADYQYHNTYFLVAHFHYTLVTGVVFACLAGLIFWYPKMMGYKLNETLNKW
                     CFWFFMIGFNVCFLPQFILGLDGMPRRLYTYMPSDGWFLLNLISTIGALLMAIGFLFL
                     VVSIVYSHFKSPREATGDNWDGLGRTLEWTTASAIPPKYNFAITPDWNDYDTFVDMKE
                     HGRHYLDNHNYKDIHMPNNTPVGFWIGIFMTIGGFFLIFETVIPALICLFGIFGTMIY
                     RSFQIDHGYHIPAAEVAETEARLREARIKEREAVSHES"
     gene            complement(2883..3983)
                     /gene="qoxA"
                     /locus_tag="P6L01_002273"
     CDS             complement(2883..3983)
                     /gene="qoxA"
                     /locus_tag="P6L01_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit II"
                     /protein_id="HDP3031319.1"
                     /translation="MSKFKSLLLLFGTLILLSGCSNIEIFNAKGPVASSQKFLILYSI
                     VFMLVICFVVLGMFAIFIYKYSYNKNAESGKMHHNAIIETIWFVIPIIIVAALAIPTV
                     KTLYDYEKPPKSEKDPMVVYAVSAGYKWFFAYPDEHIETVNTLTIPKDRPVVFKLQAM
                     DTMTSFWIPQLGGQKYAMTGMTMNWTLEASQTGTFRGRNSNFNGEGFSRQTFKVNAVS
                     QKDYDKWVKEVKGKKTLDQDTFDKQLLPSTPNKALEFNGTHMAFVDPAADPEYIFYAY
                     KRFNFELKDPNFTSEENMFKDVSDKPLIPARKAQITNANYKRHGMKLMILGNDEPYNN
                     EFKKDESKNAKEMKKISKDAQDQDNDDHGGGH"
BASE COUNT         1473 a          765 c          753 g         1319 t
ORIGIN      
        1 ttaaaatgtt gtttataagt gtgaagagtg accgccttgc ataacccaat atgttccgac
       61 aacgaaacaa agtgtaatta caagagcaaa gataactttg aatgtttgta aacgtccatc
      121 tttaccttca gttaaatgca tgaacattaa taattgaagt cctgcttgga cgaatgcaaa
      181 gccaaagata attgtcaact tcgcgtggaa tgttaatgac gtgtatagtg ttacgtaaac
      241 tgctaaaagc gttaatacga tagatgcgat aaatcctaca gtatgtttca ttattgtact
      301 catccgctat acaccatccc tatcatatat acggcagtaa agatgaaaac ccaaacaaca
      361 tctaagaagt gccagtataa acttactata aataattttg gcgcattata tttgtctaat
      421 ccgcgtcgtt ggatttggat taataaacaa atggcccaaa cgatacctag cgatacgtga
      481 caaccatgcg ttcctaatag gataaagaaa ctagaccagt aagaaccaat tgttgggtta
      541 acgccttctg atgcatagtg tgcgaattca taaatttcga atccaacaaa gactaaacct
      601 aaaagtaacg taatgatcat ccaaaacatc attaactttt gtttttcttg gcgcatgtag
      661 taaatagcaa taccacatgt gtaagaactg aataataatg caaacgtcat tattaaaaca
      721 agaggcaatt caaataactc agtagtcatt ttacctgcat aatcgccacc atgttgcaaa
      781 gttaatagtg tcgcaaatag ggtaccgaat aacgcaaatt cggctgtaat gaaaatccaa
      841 aagccaagct tatttaattc gccttcatgt gtgcgtgaat caatagtgtt tgtatcatga
      901 ctcatgactt acagcctccc tttctttaat acgcgcttct cttaatctag cttcagtttc
      961 agcaacttca gcagcaggga tatgatatcc gtgatcgatt tggaaactgc gataaatcat
     1021 agtaccaaaa ataccgaata aacaaattaa tgctggaatt acagtttcga aaattaagaa
     1081 gaaaccgccg atagtcataa agataccaat ccagaatcca acaggagtat tgtttggcat
     1141 atgaatgtct ttgtaattat ggttgtctaa gtaatgacga ccatgttctt tcatatcaac
     1201 aaatgtgtcg tagtcattcc aatctggtgt aatggcaaag ttgtatttag gtggaattgc
     1261 tgatgcagta gtccactcta gagtacggcc aaggccatcc cagttatctc cagttgcttc
     1321 acgtggagat ttgaagtgac tgtatacgat actaacaaca aggaataaga atccgattgc
     1381 cattaataat gcaccgatag ttgaaattaa gtttaataag aaccaaccat ctgatggcat
     1441 ataagtgtat aaacgacgtg gcataccatc taatccaaga atgaattgtg gtaagaaaca
     1501 aacgttaaat ccgatcatga agaaccagaa gcaccatttg tttaatgttt cgtttaattt
     1561 gtaacccatc atctttggat accagaagat taaaccagct aagcaggcaa atacaacacc
     1621 agtaaccaat gtatagtgga agtgagctac taagaagtac gtattgtgat attgatagtc
     1681 agctgatgcc attgctaaca ttacacccgt aacaccacct aaaaggaagt tagggataaa
     1741 tgctaatgag aatagcattg gtgactcaaa tgtaatacgt cctttatata atgttaataa
     1801 ccagttaaac aatttcacac cagttggaat accaatcagc attgttgaaa ttgagaagaa
     1861 tgagttgatt aacgcaccat tacccattgt gaagaaatgg tgaacccaaa ctaagaaact
     1921 aaggaacgcg ataccggcag ttgcccatac catactttga tgtccgaata aacgcttacg
     1981 agcgaatgtc gggataattt ctgagtaaat accaaatgct ggaaggataa cgatataaac
     2041 ttcagggtgc ccccataccc agaagaagtt agcccaaagc attggcatac cgccatgtgc
     2101 aactgtgaag aatgctgtgt caaatattct atcagttgtc attaatgcta acgctactgt
     2161 taaaggaggg aaagcaagaa taacaattaa tgtagtaata aatgttgtta ctgtaaacat
     2221 tggcatttgc ataaacttca tagttggtgt tttacatctt aaaattgtta caaagaagtt
     2281 gatacctgta gctaaggtac caagccctga aatttgtata gctattaagt aatagttaac
     2341 acccggacca ggactgaatt cacctgctag tggcgcatag tttgtccaac cagctgctgg
     2401 tgaaccacca ataataaatg acaggttgaa taaaatcata cctgcaaaga atagccagaa
     2461 acttacgttg tttaatactg ggaatgcaac atcacgtgct ccaatttgta atggaacaac
     2521 gatattccat aaaccaaaga taaatggcat tgccatgaag ataatcatga ttacaccatg
     2581 tgtactaaaa atttcgttat agtggttaga ttctaaaaat ttgttatcag gtactgttaa
     2641 ttgcgcacga ataagtaacg catcaatacc accacggacg aacattaata cggcacagat
     2701 taaatacata ataccgattt tcttatggtc tacagatgtg aaccattctt tgtaaagata
     2761 tttccataat ttaaagtaag taattactgc gattaaacca ataactaaga atggggcacc
     2821 aatttgtgcc attgtaatca tccagttacc tttaactagt aattgatccc atggaaaatt
     2881 cattaatgtc cacctccatg atcatcattg tcttgatctt gcgcatcttt tgaaattttc
     2941 ttcatttctt tcgcattttt cgattcatct ttcttgaact cattgttata tggttcgtca
     3001 tttccaagaa tcatcaactt cataccatgt cgtttatagt tcgcatttgt aatttgagct
     3061 ttacgagcag gtattaatgg tttgtctgat acatctttaa acatattttc ttcactagtg
     3121 aagtttggat ctttcaattc gaaattgaaa cgtttatatg catagaagat gtattctgga
     3181 tcggctgctg gatcaacaaa cgccatatgt gtaccattaa attctaaagc tttattaggt
     3241 gtgcttggta ataattgttt atcaaatgta tcttgatcta acgttttctt acctttaact
     3301 tctttcaccc atttgtcgta gtctttttga ctaacggcat ttactttaaa tgtttgacgt
     3361 gagaatcctt caccattgaa gttagagtta cgacctctga acgtaccagt ttgagatgct
     3421 tctaacgtcc aattcattgt catgccagtc atggcatatt tttgaccacc taattgtgga
     3481 atccagaaac ttgtcattgt atccatagct tgaagcttaa atacgacagg acgatcttta
     3541 gggattgtta atgtattaac agtctctata tgttcatctg gataagcaaa gaaccatttg
     3601 tatcctgcac ttactgcata tacaaccatt ggatctttct cactcttcgg tggtttttcg
     3661 taatcgtata aagttttaac tgtaggaata gctaaagcag caacgattat gataggtatt
     3721 acaaaccata ttgtttcaat gatggcatta tggtgcatct taccagattc ggcattctta
     3781 ttataactat acttgtaaat aaaaatggcg aacatgccaa gtacaacgaa acaaataaca
     3841 agcatgaaga cgattgaata aagaatcaag aacttctgac tacttgctac tggccctttt
     3901 gcgttgaaaa tttctatatt tgaacaacca ctaagtaaaa ttagtgtgcc aaataataga
     3961 agcaaagact taaattttga cacttttttg acctcctaat actacaaatg tagggcttaa
     4021 cattaatttt aagttattac acaatattta caagggctta tgggaaaaaa attaataaaa
     4081 ttgtatcaaa aatgttgata aatcaaggtg tgacgtgggt tcacacattt gttaaaatta
     4141 tgtgtacatt ttgtgactaa tagcgttttt gaaaaaattg cttcatattt aaaaggaaag
     4201 ttgagaatag ctttcaatta aaggttaatt gaatgaaaat aaaaaaagtt tgaaattgtc
     4261 taataaattg aaaatgatgg ctagttcatt gaaaggaagc gcaaacactt
//
LOCUS       DAOBBH010000188         4299 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.188, whole genome shotgun sequence.
ACCESSION   DAOBBH010000188 DAOBBH010000000
VERSION     DAOBBH010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.188"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..536
                     /locus_tag="P6L01_002274"
     CDS             <1..536
                     /locus_tag="P6L01_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HDP3031320.1"
                     /translation="DTALKQSTSENNSLSKLEDVQVKDGDNSKNKGPVYVDVKGAVKH
                     PNVYKMTSKDRVVDLLDKAQLLDDADVSRINLSEKLTDQKMIFIPHKGQKNVEPQIEV
                     NSVHVKNGNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKV
                     KGFGSKTFDKLKSYFTI"
     gene            628..1089
                     /locus_tag="P6L01_002275"
     CDS             628..1089
                     /locus_tag="P6L01_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDP3031321.1"
                     /translation="MERIKWEEYFMAQSHLLALRSTCQRLSVGATIVKDNRIIAGGYN
                     GSVAGEVHCIDEGCLIEDGHCIRTIHAEMNALLQCAKQGVSTEGATIYVTHFPCLNCT
                     KSIIQAGIKRIYYAEDYHNHEYATKLLKQSGIEFKKIPFSPEYVAKYLTKG"
     gene            1094..3295
                     /locus_tag="P6L01_002276"
     CDS             1094..3295
                     /locus_tag="P6L01_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDP3031322.1"
                     /translation="MLYVALSMIVGVLWNSSKVLSTFLFILLLYITYRKNKIVYAPIS
                     LFLIIFSSWYLHYSQQAIFNYINYIERNSQFNERAQVIQIQRQGSDTYKGRLSLKNEI
                     YPFFLTDKKNFDLKKIESRNCIVKGQFKVNENKFVTLKLQSIVVQSCLESNRSNLIEK
                     HKQFIMNRIYDSGIKFPDRIMALITGDVKEINEQFKERVKEIGIYHLLAVSGSHIAAI
                     VFLIYQPLKRLNLPLFVIKGITIIVLTLYAQYTNYAPSAVRAIIMTTLVLLITKQIKI
                     KGIQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQQLSKLQSLFIITFI
                     AQLASFIVAIPNFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYL
                     VDLSFNFHDWLLDLFTRIKQSHFSVPKFNDWIFIIFIISVYYIFWLLAKRKYILVTFW
                     TIIILTLLITFPTNSHHKITMLNVGQGDSILYEGGKNQNVLIDTGGKVIDDTKQPSYS
                     ISKYHILPTLNERGINELEYLILTHPHNDHIGEVEYIISHIKIKHIVIYNKGYSSNTL
                     MLLSKLSHKYNIKLMDVRQVSSFKLGDSSFLFFDSFIPNSRDKNEYSIITMITYQNKK
                     VLLMGDASKNNESLLLKKYNLPEIDILKVGHHGSKTSSSKEFIEMIKPKISLISSGKN
                     NMYHLPNIEVVKRLQRIRSRIYNSQQNGQVTIDLDDNLKVDSNSYGNASGL"
     gene            3352..>4299
                     /gene="holA"
                     /locus_tag="P6L01_002277"
     CDS             3352..>4299
                     /gene="holA"
                     /locus_tag="P6L01_002277"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDP3031323.1"
                     /translation="MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYE
                     TEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKAPKDMAHNVDQLIEFIEKYDGENL
                     IVFEIYQNKLDERKKLTKTLKKHARLKKIEQMSEEEIKKWIQSKLNENFKDIKRDALD
                     LFIELTGINFNIVSQEIEKLILFLGDRPTINKQDVNQIINRSLEQNVFLLTEYIQKRK
                     KEQAIHLVKDLITMKEEPIKLLALITSNYRLFYQCKILSQKGYSGQQIAKTIGVHPYR
                     VKLALGQVRHYQLDELLNIVDACAETDYKLKSSYMDKQLI"
BASE COUNT         1609 a          533 c          704 g         1453 t
ORIGIN      
        1 aagatactgc tctgaaacaa agcactagtg aaaataatag tttgtccaaa ttagaagatg
       61 tccaggtcaa agatggagat aattccaaaa ataaaggtcc tgtatatgtc gatgtaaaag
      121 gtgctgttaa acatcctaat gtttataaaa tgacatctaa ggatagagta gttgatttac
      181 ttgataaagc acaattattg gatgatgcag atgtaagtcg aattaatttg tctgaaaaat
      241 taacagatca aaaaatgatt ttcatacctc ataaaggaca aaagaatgtt gaaccacaaa
      301 ttgaagtaaa cagtgtgcac gtaaaaaatg ggaacacaaa taatactaaa gtaaatttaa
      361 ataccgcatc tgtatcagaa ttgatgtctg ttcctggagt agggcaagct aaagctaatg
      421 caattgttga atatcgcaac caacaaggtg catttcaaga aattgacgat ttgaaaaaag
      481 taaaaggttt tggaagtaaa acttttgata aactgaaatc ttatttcacg atataatgga
      541 ttattttaat tgcaaatttt attttttgtg attaatctta tattattaga aaatgttact
      601 aaattttaag tatggaaggt gaacatcttg gaaagaatca aatgggaaga atattttatg
      661 gcacaaagtc atttgctagc attacgttca acttgtcaaa gattatctgt aggtgcaacg
      721 attgttaagg ataatcgtat tattgctggt ggttataatg gctctgtagc tggcgaggtg
      781 cattgtatag atgaaggatg tttaattgaa gatggacatt gtatcagaac gatacatgca
      841 gaaatgaatg ctttattaca atgtgcaaaa caaggtgtat ctactgaagg tgcaacaatc
      901 tatgttactc attttccatg cctaaattgt acaaagtcaa ttattcaagc aggtataaag
      961 cgtatctact atgcagaaga ttatcataac catgaatatg caactaaatt actcaaacaa
     1021 tctggtattg aatttaaaaa aattccattt tcaccagaat atgttgctaa atatctgact
     1081 aaaggttaat gttttgctgt atgtcgcgtt atcaatgatt gtaggagtgc tttggaattc
     1141 tagcaaagtg ctctctacat ttcttttcat tttacttttg tatattactt atcgtaaaaa
     1201 taaaatcgtt tatgccccta tttctctctt tttaatcatt ttctcctcat ggtatttaca
     1261 ttattcacaa caagcaatat ttaattatat caattatatt gaacgtaatt ctcagtttaa
     1321 tgagcgtgct caagtaatcc aaattcaacg tcaaggtagt gacacatata aaggtaggtt
     1381 gagtttaaaa aatgaaatat atcctttctt tttaacagat aaaaagaatt ttgatttaaa
     1441 gaaaattgaa agtcgtaatt gtattgttaa aggacaattc aaagttaatg aaaataagtt
     1501 tgtaactctt aaattacaaa gtatagttgt acaaagctgc ctagaatcga accggtctaa
     1561 tttaattgag aaacataaac agtttataat gaatcgaatt tatgattcgg gtattaagtt
     1621 tccggatcgt attatggcat tgattactgg tgacgtaaaa gaaattaatg agcaatttaa
     1681 ggaacgtgtt aaagagatag gtatatatca tttgctggca gttagtggct cgcatatagc
     1741 tgcaattgta ttcttaattt accaaccttt aaaacgatta aatttacctt tatttgtcat
     1801 taaaggaatt acaatcattg tattaacttt atatgctcaa tacacaaatt atgcacctag
     1861 tgctgtaaga gctataataa tgacaactct tgtactgctt attactaagc aaattaaaat
     1921 aaagggtatt cagctattag catttgcatt tataattatg tttattttaa acccactagt
     1981 tgtttatgat attggatttc aattttcatt catcatttca ttttttatta tgctactttt
     2041 tcctttttta cagcaattgt caaagttaca atcattattc ataattacgt ttattgcaca
     2101 attagcttca tttatcgttg ccattccaaa ctttcatcaa cttcaatggg tgggattttt
     2161 atctaatttg atttttgtac cgtactattc gattatattg tttccgctat ctattttatt
     2221 ctttattaca agtcatttta ttgtgggatt aacgccgcta aattacttgg ttgacctaag
     2281 ttttaatttt catgactggt tactagacct attcacaaga attaagcaat cacatttttc
     2341 tgttcccaag tttaatgatt ggatatttat aatatttata atttctgttt attacatatt
     2401 ttggttattg gctaaacgta aatatatatt ggttacgttt tggactataa ttattctgac
     2461 attattaata acgtttccaa caaattcaca tcacaaaatt acaatgttaa atgtggggca
     2521 gggagacagt attttatatg aaggcggtaa gaaccaaaat gtcttgattg atacaggtgg
     2581 gaaagtgatt gatgatacta aacaacctag ttattcaatt tctaaatatc atattttacc
     2641 aacgctaaat gaaagaggga taaatgaatt agagtatcta attttaacac atccacacaa
     2701 tgaccatatt ggtgaagtgg aatatattat tagtcatatt aaaattaaac atatagtgat
     2761 atacaataag ggatatagta gtaatacatt gatgttatta tcgaaattaa gccataagta
     2821 caacattaaa cttatggatg taagacaagt tagtagtttt aaacttggag atagtagttt
     2881 tctatttttt gatagtttta ttccaaatag ccgagataaa aatgagtatt cgattattac
     2941 tatgattaca tatcaaaata aaaaagtttt attaatgggc gatgctagta aaaataatga
     3001 atctttacta ctaaaaaaat ataacttgcc ggagattgat attttaaaag taggacatca
     3061 tgggagcaag acaagtagtt ctaaagaatt tatagagatg attaagccta aaataagttt
     3121 gatttcttct gggaagaaca atatgtatca tcttcctaat atagaagttg ttaaacgatt
     3181 gcaaaggatt cgcagtcgca tttacaatag tcaacaaaac ggtcaagtta caattgactt
     3241 agatgataat ttaaaagttg attcaaactc ttatggaaat gcaagtggtt tatagataca
     3301 gaaacatgat ataatctatt gatgtgtcaa acgaatgaaa gggtgtaatg aatgagcgac
     3361 aatattgtag ctatttatgg agatgtgcct gaattggttg aaaaacaaag tgcagaaatc
     3421 atatcacaat ttttgaaaag tgatagagat gactttaact ttgtgaaata taatttatac
     3481 gaaacagaga ttgcaccaat tgttgaagaa acattaacat tgcctttctt ttcagataaa
     3541 aaagcaattt tggttaaaaa tgcatatata tttacaggtg aaaaagcgcc aaaagatatg
     3601 gctcataatg tagatcaatt aatagaattt attgaaaaat atgatggcga aaatttgatt
     3661 gtctttgaga tatatcaaaa taaacttgat gaaagaaaaa agttaactaa aactctaaaa
     3721 aagcatgcaa ggcttaaaaa aatagagcaa atgtctgaag aagaaataaa aaaatggatt
     3781 caaagtaaat taaatgagaa tttcaaagat atcaaaagag atgcattaga tttatttatt
     3841 gagttgacag gtattaactt taatattgtc tcacaagaga tagaaaagtt gattttattt
     3901 ttaggcgata gaccaacaat taataagcag gatgttaacc aaattattaa tagaagttta
     3961 gagcaaaatg tatttttact gactgaatac attcagaaaa gaaagaaaga acaagcaatt
     4021 catttagtaa aagatttaat aactatgaaa gaagaaccaa ttaaattact tgcactaatt
     4081 acaagtaatt accgattatt ttatcaatgt aagattctga gtcaaaaagg atatagtgga
     4141 cagcaaattg ctaaaacaat aggagtgcat ccatacagag taaaattagc gttaggacaa
     4201 gtaagacatt atcaacttga tgaattattg aatattgtag atgcttgtgc ggaaactgat
     4261 tataaactta aatcatcata tatggataaa cagttaata
//
LOCUS       DAOBBH010000189         4287 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.189, whole genome shotgun sequence.
ACCESSION   DAOBBH010000189 DAOBBH010000000
VERSION     DAOBBH010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.189"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..393
                     /locus_tag="P6L01_002278"
     CDS             <1..393
                     /locus_tag="P6L01_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDP3031324.1"
                     /translation="HDATKPYDKTYDKILVDAPCSGLGVMRHKPEIKYTQSKQHIESL
                     VELQLEILENVKNNVKIGGEIIYSTCTIEQLENENVIYTFLKNNKNFEFEPFQHPITG
                     ELVKTLQIMPQDFNSDGFFITKIKRKDN"
     gene            396..1490
                     /gene="rlmN"
                     /locus_tag="P6L01_002279"
     CDS             396..1490
                     /gene="rlmN"
                     /locus_tag="P6L01_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002435140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDP3031325.1"
                     /translation="MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAK
                     QIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQD
                     GYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
                     LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIY
                     DFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYG
                     LFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLG
                     INATIRREQGSDIDAACGQLRAKERQVETR"
     gene            1497..2240
                     /locus_tag="P6L01_002280"
     CDS             1497..2240
                     /locus_tag="P6L01_002280"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase Stp1"
                     /protein_id="HDP3031326.1"
                     /translation="MLEAQFFTDTGQHRDKNEDAGGIFYNQTNQQLLVLCDGMGGHKA
                     GEVASKFVTDELKSRFEAENLIEEHQAENWLRNNIKDINFQLYHYAQENAEYKGMGTT
                     CVCALVFEKSVVIANVGDSRAYVINSRQIEQITSDHSFVNHLVLTGQITPEEAFTHPQ
                     RNIITKVMGTDKRVSPDLFIKRLNFYDYLLLNSDGLTDYVKDNEIKRLLVKEGTIEDH
                     GDQLMQLALDNHSKDNVTFILAAIEGDKV"
     gene            2237..4231
                     /gene="pknB"
                     /locus_tag="P6L01_002281"
     CDS             2237..4231
                     /gene="pknB"
                     /locus_tag="P6L01_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase Stk1"
                     /protein_id="HDP3031327.1"
                     /translation="MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIP
                     PREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESH
                     GPLSVDTAINFTNQILDGIKHVHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSE
                     TSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIA
                     IKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRA
                     NEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEG
                     TVYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQ
                     IFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNV
                     IGLPKEEALQKLKSLGLKDVTIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYES
                     LGIKQVYVEDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEG
                     STISFVVSKGKKSDSSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFD
                     ITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDI"
BASE COUNT         1623 a          587 c          842 g         1235 t
ORIGIN      
        1 catgatgcga caaaacctta tgataaaaca tacgataaga tacttgttga tgcaccatgt
       61 agcggattag gtgtaatgag acataagccg gagattaagt atactcaaag caaacaacat
      121 attgagtcac tagttgaatt acagcttgaa atattggaaa atgtaaaaaa caatgtaaaa
      181 ataggtggcg aaatcatcta ttcaacatgt acaattgagc aactagaaaa tgaaaacgtg
      241 atttatacgt ttctgaaaaa taataaaaac ttcgaatttg aaccgtttca acatccgata
      301 actggagagt tggtcaaaac gttacaaatc atgccgcaag actttaattc agatggattc
      361 tttatcacta agataaaaag aaaggacaat taggaatgat aactgctgaa aagaaaaaga
      421 agaataaatt tcttccaaat tttgacaagc aatcaatata ttcattgcga tttgacgaaa
      481 tgcaaaactg gctcgttgaa caaggtcaac aaaaatttcg agcgaaacag atttttgaat
      541 ggttatatca aaaaagagta gattcgattg atgaaatgac gaacttatcg aaagacttac
      601 gacagctttt aaaagataac tttactgtta caactttaac aactgtagta aaacaagaaa
      661 gtaaagacgg tacaattaaa ttcttatttg aattacaaga tggctataca attgaaactg
      721 ttttaatgag acatgattat ggaaattcag tatgtgtaac gacacaagta ggttgtcgta
      781 tcggatgtac gttttgtgct tctacacttg gcggcttaaa aagaaacctt gaagctggcg
      841 aaattgtttc acaagtttta acagttcaaa aagcccttga tgctacagaa gagcgcgtat
      901 ctcaaattgt cataatgggt atcggtgaac catttgaaaa ttatgatgaa atgatggact
      961 ttttaagaat cgtcaatgat gataacagtt taaatattgg tgcacgtcat attacagtat
     1021 caacatcagg tatcattcct agaatatatg actttgcgga tgaagatatc caaattaatt
     1081 ttgctgtaag cttacacgcc gcaaaagatg aagtgcgatc acgcttgatg ccaattaacc
     1141 gtgcatataa tgttgagaag ttaatcgaag caattcaata ttatcaagaa aaaacaaatc
     1201 gtcgtgttac ttttgaatat ggtctgtttg gtggtgtgaa tgaccaacta gaacatgcaa
     1261 gagaattagc acatttaata aaaggcttaa actgccatgt taacttaatt cctgtcaacc
     1321 atgttccaga aagaaattat gtgaaaacgg ctaaaaatga tatctttaaa tttgaaaaag
     1381 aattaaagag actaggaatt aatgccacaa tacgtcgtga acaaggttcg gatattgacg
     1441 cagcttgtgg tcaattaaga gcaaaggaac gacaagtaga aacgaggtaa agacaaatgc
     1501 tagaggcaca attttttact gatactggac aacatagaga taagaatgaa gatgcgggtg
     1561 gtatttttta taatcaaact aatcaacaac ttttagttct gtgtgatggt atgggcggcc
     1621 ataaagcagg agaagttgca agtaaatttg ttacagatga gttgaaatcc cgttttgaag
     1681 cggaaaatct tatagaagaa catcaagctg aaaattggtt gcgtaataat ataaaagata
     1741 taaattttca gttatatcac tatgcacaag aaaatgcaga atataaaggt atgggtacaa
     1801 catgtgtttg tgcacttgtt tttgaaaaat cagttgtgat agcaaatgtc ggtgattcta
     1861 gagcctatgt tattaatagt agacaaattg aacaaattac tagtgatcac tcatttgtta
     1921 atcatcttgt tttaacgggt caaattacgc cggaagaagc atttacacat ccacaacgta
     1981 atattattac gaaggtgatg ggcacagata aacgtgtgag tccagatttg tttattaagc
     2041 gattaaattt ttatgattat ttattattaa attcagatgg attaactgat tatgttaaag
     2101 acaatgaaat taagcgtttg ttagtaaaag aaggtacaat agaagatcat ggtgatcaat
     2161 taatgcaatt ggcattagat aaccattcga aagataacgt tactttcata ctcgcggcta
     2221 ttgaaggtga taaagtatga taggtaaaat aataaatgaa cgatataaaa ttgtagataa
     2281 gcttggcggc ggtggcatga gtaccgttta tcttgcagaa gatacgatac ttaacattaa
     2341 agttgcaatt aaggcgattt ttataccacc tagagaaaaa gaagaaacat taaaacgttt
     2401 tgaacgagaa gtacataact catcacagct atcacatcaa aatatagtaa gtatgatcga
     2461 tgttgatgaa gaagatgact gttactactt agtaatggaa tatattgaag gtccgacttt
     2521 gtctgagtat attgaaagtc atgggccatt aagtgttgac acggcgatta attttacgaa
     2581 tcaaatattg gatggcatta aacatgtgca tgatatgcgt attgtacata gagatattaa
     2641 gccacaaaat atattaattg acagcaataa aacgttgaaa atatttgatt ttggaattgc
     2701 taaagcttta agtgagacgt ctttaactca gactaatcat gtgttaggta ctgtgcagta
     2761 cttttcgcca gaacaagcaa aaggtgaggc aacggatgaa tgtacagata tttattctat
     2821 aggtattgtg ttatatgaaa tgcttgttgg tgaaccaccc tttaatggag aaactgcagt
     2881 tagcattgcg attaaacata ttcaggattc tgtgccaaat gtgacaacag atgtacgtaa
     2941 ggatattccg caatctttaa gtaatgtcat tttacgcgct acagaaaaag acaaagcgaa
     3001 tcgttacaaa acaattcaag aaatgaaaga tgatttgagt agtgttttac atgaaaatcg
     3061 agcgaatgaa gatgtctatg aactcgataa aatgaaaacg atagcggtac ctttgaaaaa
     3121 agaagatcta gcaaagcata ttagtgaaca taagtcgaat caacctaaac gtgaaacgac
     3181 gcaagtacct attgtaaatg ggcctgctca tcatcagcaa ttccaaaagc cagaaggtac
     3241 ggtgtacgaa ccaaaaccta aaaagaaatc aacacgaaag attgtgctct tatcactaat
     3301 cttttcgttg ttaatgattg cacttgtttc ttttgtggca atggcaatgt ttggtaataa
     3361 atacgaagag acacctgatg taatcgggaa atctgtaaaa gaagcagagc aaatattcaa
     3421 taaaaacaac ctgaaattgg gtaaaatttc tagaagttat agtgataaat atcctgaaaa
     3481 tgaaattatt aagacaactc ctaatactgg tgaacgtgtt gaacgtggtg acagtgttga
     3541 tgttgttata tcaaagggcc ctgaaaaggt taaaatgcca aatgtcattg gtttacctaa
     3601 ggaggaagca ttgcagaaat taaaatcgtt aggtcttaaa gatgttacga ttgaaaaagt
     3661 atataataat caagcgccaa aaggatacat tgcaaatcaa agtgtaaccg caaatactga
     3721 aatcgctatt catgattcta atattaaact atatgaatct ttaggcatta agcaagttta
     3781 tgtagaagac tttgagcata aatcctttag caaagctaaa aaagccttag aagaaaaagg
     3841 gtttaaagtt gaaagtaagg aagagtatag tgacgatatt gatgagggtg atgtgatttc
     3901 tcaatctcct aaaggaaaat cagtagatga ggggtcaacg atttcatttg ttgtttctaa
     3961 aggtaaaaaa agtgactcat cagatgtcaa aacgacaact gaatcggtag atgtaccata
     4021 cactggtaaa aatgataagt cacaaaaagt taaagtttat attaaagata aagataatga
     4081 cggttcaact gaaaaaggta gtttcgacat tactagtgat caacgtatag acattccttt
     4141 aagaattgaa aaaggaaaaa cagcaagtta tattgttaaa gttgacggta aaactgtagc
     4201 tgaaaaagaa gtcagctatg atgatattta aatataattg aagtaaatgt accgaggttt
     4261 ctatttggaa gtctcggtat ttttatg
//
LOCUS       DAOBBH010000190         4282 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.190, whole genome shotgun sequence.
ACCESSION   DAOBBH010000190 DAOBBH010000000
VERSION     DAOBBH010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.190"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            92..1219
                     /locus_tag="P6L01_002282"
     CDS             92..1219
                     /locus_tag="P6L01_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDP3031328.1"
                     /translation="MKSKYEPLFDKVELPNGVELRNRFVLAPLTHISSNDDGTISDVE
                     LPYIEKRSQDVGITINAASNVSDVGKAFPGQPSIAHDSDIEGLKRLATAMKKNGAKAL
                     VQIHHGGAQALPELIPDGDVVAPSPISLKSFGQKQEHSAREMTNEEIEQAIKDFGEAT
                     RRAIEAGFDGVEIHGANHYLIHQFVSPYYNRRNDVWANQYKFPVAVIEEVLKAKEAYG
                     NKDFIVGYRLSPEEAESPGITMEITEELVNKISHMPIDYIHVSMMDTHATTREGKYAG
                     QERLPLIHKWINGRMPLIGIGSIFTADEALDAVENVGVDLVAIGRELLLDYQFVEKIK
                     DGREDEIINYFDPEREDNHHLTPNLWHQFNEGFYPLPRKDK"
     gene            1527..2717
                     /locus_tag="P6L01_002283"
     CDS             1527..2717
                     /locus_tag="P6L01_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HDP3031329.1"
                     /translation="MTKSEKIIELTNHYGAHNYLPLPIVISEAEGVWVKDPEGNKYMD
                     MLSAYSAVNQGHRHPKIIQALKDQADKVTLVSRAFHSDNLGEWYEKICKLAGKDKALP
                     MNTGAEAVETALKAARRWAYDVKGIEPNKAEIIAFNGNFHGRTMAPVSLSSEAEYQRG
                     YGPLLDGFRKVDFGDVDALKAAINENTAAVLVEPIQGEAGINIPPEGYLKAIRELCDE
                     HNVLFIADEIQAGLGRSGKLFATDWDNVKPDVYILGKALGGGVFPISVVLADKEVLDV
                     FTPGSHGSTFGGNPLACAASIAALDVIVDEDLPGRSLELGDYFKEQLKQIDHPSIKEV
                     RGRGLFIGVELNESARPYCEALKEEGLLCKETHDTVIRFAPPLIITKEELDLALEKIR
                     HVFQ"
     gene            2826..4070
                     /locus_tag="P6L01_002284"
     CDS             2826..4070
                     /locus_tag="P6L01_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HDP3031330.1"
                     /translation="MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIP
                     VRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPY
                     GGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDE
                     YSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQG
                     FGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETI
                     SNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILL
                     VPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVNEKLREKLEAAFDTIYELSQNRKIDM
                     RLAAYIIGIKRTAEAARYRGWA"
BASE COUNT         1497 a          619 c          894 g         1272 t
ORIGIN      
        1 taaacgaatt gcaactatat aaacagataa ttggagaatg aaaaaattac atgttatagt
       61 caactcaata attttaagga ggaattaagt aatgaaaagt aaatacgaac cattgtttga
      121 taaagtagaa ttaccaaatg gagtagagtt gagaaatcga tttgtgttag cccctttaac
      181 acatatttct tcaaatgatg atggtactat ttcagatgta gaacttcctt atattgaaaa
      241 gcgttcacaa gatgttggta ttacaattaa tgctgcgagt aatgtgagtg atgtcggaaa
      301 agcatttcca ggacagccgt caatcgcgca tgacagtgat attgaaggac taaaacgatt
      361 agctacagca atgaagaaaa acggtgccaa agcactcgta caaatacatc atggtggtgc
      421 acaagcattg cctgaattaa tacctgatgg agacgtcgta gcaccaagtc caatttcttt
      481 aaaaagtttc ggtcagaaac aagaacatag tgctagagaa atgacgaatg aagagattga
      541 acaagcaatc aaggattttg gtgaagcaac acgacgtgca attgaagcag ggtttgatgg
      601 tgttgaaata catggcgcga atcattactt aattcatcaa tttgtatcac catactataa
      661 tagaagaaat gatgtatggg caaatcaata taaattcccg gtcgctgtga ttgaagaagt
      721 gcttaaagcg aaagaagcgt atggcaataa agactttata gttggataca gattatctcc
      781 agaggaagcg gagtctccag gaatcacaat ggaaattaca gaggaactcg ttaataaaat
      841 tagccatatg ccaatcgact atattcatgt ttcaatgatg gatacgcatg caacgacacg
      901 tgaaggtaaa tacgctggac aagaaagact acctttaatt cacaaatgga taaatggtcg
      961 tatgccactt atcggtattg gttcaatttt cacagctgac gaagctttag atgcagttga
     1021 aaatgttggt gttgacttag tagccattgg tagagagcta ctactggatt atcaatttgt
     1081 tgaaaaaatt aaagatggac gggaagatga aattattaat tactttgatc cagagagaga
     1141 agataatcat cacttaactc ctaatttatg gcatcaattt aatgaaggat tctatccatt
     1201 accacgtaaa gataaataag tgtttaactg ttaccactaa tgatattgtg tatgcaattt
     1261 gttagtggtt ttcaatattt ataaataaaa atattcttag taaaatacac ttttgattaa
     1321 gtgtaaaatt atcaattctt ttgcttgtaa tcatttaagt aattatgtca ttataaatgt
     1381 aagggttttc aaaatagaca aatttaatga aacttagtga ttatttcatc ttttttggtg
     1441 gaaaagtatc tgctttgaaa actaatgtaa ttatttggat aatgtttcat gtataatagt
     1501 tataaatgat gaaaagggga gatatgatga ctaaatctga aaaaattatt gagttaacaa
     1561 atcattacgg agcacataat tatttaccat tgccaattgt catttcagaa gctgaagggg
     1621 tatgggttaa agatcctgaa ggcaataaat atatggatat gttatctgca tattccgctg
     1681 ttaaccaagg tcatagacat ccgaaaatta ttcaagcatt aaaagatcaa gctgataaag
     1741 tgactctagt ttcacgtgct tttcatagtg ataacttagg tgaatggtac gaaaaaattt
     1801 gtaaactggc aggtaaagat aaagctttac caatgaatac aggtgctgaa gcagtagaaa
     1861 cagctttgaa agcagcacga cgctgggcat acgatgttaa aggaattgag ccaaataaag
     1921 cagaaatcat tgcatttaat ggtaacttcc atggtcgaac aatggcgcca gtttcattat
     1981 cttcagaagc agaataccaa cgtggttatg gtccgttatt agatggattt agaaaagttg
     2041 attttggaga tgtagatgca ttgaaagctg caattaatga aaatactgca gcagttttag
     2101 tagaaccaat tcaaggtgaa gcgggtataa atataccgcc agaaggatat ttgaaagcaa
     2161 ttagagaatt atgtgatgaa cataatgtct tatttattgc tgacgaaatc caagcaggat
     2221 taggtcgttc gggtaaatta tttgctacgg attgggataa tgtaaaacct gatgtctata
     2281 ttttaggtaa agcactaggt ggtggtgtct tcccaatttc tgttgtatta gcagataaag
     2341 aagtattaga tgtctttaca cctggctcac atggttcaac atttggtggt aatccacttg
     2401 cttgtgctgc atcaattgct gcattagatg ttatcgttga tgaggattta ccagggcgct
     2461 ctttagaatt aggagattat tttaaagaac aattaaagca aattgatcat ccatcaatta
     2521 aagaagtccg tggacgtggt ttgtttatag gtgtggaact taatgaaagt gctagaccat
     2581 attgtgaagc tttgaaagaa gaaggcttat tatgtaaaga aacgcatgat actgtcattc
     2641 gttttgcacc accattaatt attactaaag aagaattgga ccttgcactt gaaaaaataa
     2701 gacatgtatt tcaataatgt caaaaagttg taacaatcag tttgtaaggg ctttaaaaag
     2761 gaatacgtta tgttacaata tgtttgaaag cgaaatcatt aggtaaagaa gaggcgaaaa
     2821 ggatcatgac tgagaacaat aatttagtaa cttctactca aggaattatt aaagaagcat
     2881 tgcataaatt gggatttgac gaaggaatgt acgatttaat taaagaacct ttaagaatgt
     2941 tacaagtgcg tatccctgta cgaatggatg atggcacagt taaaacattc acaggttacc
     3001 gtgcgcaaca taatgatgct gttggaccaa caaaaggggg cgtgcgtttc cacccagatg
     3061 ttgatgaaga agaagtaaaa gcattatcaa tgtggatgac tttgaaatgt ggcattgtaa
     3121 acttaccata cggtggtggt aagggtggta tcgtttgtga tccacgtcaa atgagcattc
     3181 atgaagttga acgtttatca cgcggatatg taagagcaat ttcacaattc gtaggtccga
     3241 acaaagatat tccagcacca gatgtattta caaactcaca aattatggct tggatgatgg
     3301 atgaatatag tgcattagat aaatttaatt caccaggttt catcacaggt aaaccaattg
     3361 tattgggtgg ttctcatgga cgcgacagat caactgcact aggtgtagtt attgcaattg
     3421 aacaagctgc aaaacgtcgt aatatgcaaa ttgaaggtgc caaggttgtt attcaaggtt
     3481 tcggtaatgc cggaagtttc ttagctaaat tcttatatga tttaggtgca aaaattgtag
     3541 gtatctctga tgcttacggt gcattacacg atccaaatgg cttagatata gattatttat
     3601 tagaccgtcg tgatagtttt ggtacggtaa caaatttatt tgaagaaaca atctcaaata
     3661 aagaattgtt tgaattagat tgtgacattt tagtaccagc ggctatttca aaccaaatta
     3721 cagaagacaa tgcacatgat attaaagcta gtatcgttgt tgaagctgct aatggaccta
     3781 caacaccaga agcaacacgt attttaactg aacgtggtat attattagtt ccagacgtat
     3841 tagcaagtgc tggtggtgta acggtttctt acttcgaatg ggtacaaaat aatcaaggtt
     3901 attattggtc tgaagaagaa gtaaatgaaa agctacgtga aaaattagaa gcggcatttg
     3961 acacgattta cgaattgtct caaaatcgaa aaatagatat gagacttgca gcatatatca
     4021 taggtattaa acgtacagca gaagcagcta gatatcgtgg ttgggcataa ttaattatca
     4081 tatgtgattt aacgagcttg ggacagaaaa caaagcccta agctcgttaa ttttatttta
     4141 gcagcagttg actgtaaaac aatgcccgtg taacacgctc ttttcaaaaa tagtcggggc
     4201 cccaacacag aggctggtgg aaagtcagct tacaatagtg tgcaagttgg ctggccccca
     4261 acactgagaa tttttaatta aa
//
LOCUS       DAOBBH010000191         4226 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.191, whole genome shotgun sequence.
ACCESSION   DAOBBH010000191 DAOBBH010000000
VERSION     DAOBBH010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.191"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            350..1153
                     /locus_tag="P6L01_002285"
     CDS             350..1153
                     /locus_tag="P6L01_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDP3031331.1"
                     /translation="MKKIATATIATAGFATIAIASGNQAHASEQDNYGYNPNDPTSYS
                     YTYTIDAQGNYHYTWKGNWHPSQLNQDNGYYSYYYYNGYNNYNNYNNGYSYNNYSRYN
                     NYSNNNQSYNYNNYNSYNTNSYRTGGLGASYSTSSNNVQVTTTMAPSSNGRSISSGYT
                     SGRNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAARAGYTVNNTPKAGAIMQTTQ
                     GAYGHVAYVESVNSNGSVRVSEMNYGYGPGVVTSRTISASQAAGYNFIH"
     gene            complement(1171..1413)
                     /locus_tag="P6L01_002286"
     CDS             complement(1171..1413)
                     /locus_tag="P6L01_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031332.1"
                     /translation="MTKLIENLKASIFVSTLTKNLSMYSRFDMPKFGCVNGCMLFILY
                     YRVNRIGENRNFEIFTNSIFFIEIKKANKAFETLLA"
     gene            complement(1587..2987)
                     /gene="nhaC"
                     /locus_tag="P6L01_002287"
     CDS             complement(1587..2987)
                     /gene="nhaC"
                     /locus_tag="P6L01_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDP3031333.1"
                     /translation="MMKRKPTFLESISTMIVMVIVVVTGFVFFDIPIQVLLIIASAYA
                     TWIAKRVGLTWQDLEKGIAERLNTAMPAILIILAVGIIVGSWMFSGTVPALIYYGLDL
                     LNPSYFLISAFFISAVTSVATGTAWGSASTAGIALISIGNQLGIPPGMAAGAIIAGAV
                     FGDKMSPLSDTTNLAALVTKVNIFKHIHSMMWTTIPASIIGLLVWFIAGFQFKGHSND
                     KQIQTLLSELAQIYQINIWVWVPLIVIIVCLLFKMATVPAMVISSFSAIIVGTFNHHF
                     KMTDGFKATFSGFNDSMIHQSHISSSVKSLLEQGGMMSMTQILVTIFCGYAFAGIVEK
                     AGCLEVLLTTISKGIHSVGSLICITVICCIALVFAAGVASIVIIMVGVLMKDLFEKYQ
                     VSRSVLSRTLEDSSTMVLPLIPWGTSGIYYTNQLHVSVGEFFMWTVPCYLCAIIAIIY
                     GFTGIGIKKSSNSRLT"
     gene            complement(3082..4164)
                     /locus_tag="P6L01_002288"
     CDS             complement(3082..4164)
                     /locus_tag="P6L01_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HDP3031334.1"
                     /translation="MKIAIVGSGNGAVTAAVDMVSKGHDVKLYCRNQSISKFQNAIEK
                     GGFDFNNEGDERFVKFTDISDDMEYVLKDAEIVQVIIPSSYIEYYADVMAEHVTDNQL
                     IFFNMAAAMGSIRFMNVLEDRHIETKPQLAEANTLTYGTRVDFENAAVDLSLNVRRIF
                     FSTYDRSCLNDCYDKVSSIYDHLVKEESLIKTNLENGNPEVHPGPTLLNVGRIDYAGE
                     FALYKEGITKHTVRLLHAIELERLNLGRRLGFELSTAKESRIERGYLERDKEDEPLNR
                     LFNTSPVFSQIPGPNHVESRYLTEDIAYGLVLWSSLGRVIDVPTPNIDAVIVIASTIL
                     ERDFFEEGLTVEEIGLDKLDLEKYLK"
BASE COUNT         1462 a          827 c          595 g         1342 t
ORIGIN      
        1 ttttatgcat tttctgatta aaagtcctat attatgcaca aaatatacat ttagaatttt
       61 cagtaatatt acaaatttgt aacagactta ttttcgaaac attcagtttt cacaaagaat
      121 gccgtagtta taagttttgc aaaatgcaaa tttctatact tttaaaattc tattgtgtca
      181 agatattagc aaaaaattac aaaacgttaa caaagcacac aaggacgcta atttctgtta
      241 tagtctttct tgtcgtttaa ttacgagaga taacttataa cattaatttt gtcacacgga
      301 cattacgaaa atattttaga tttaaaattt ttaggaggat atttttaaaa tgaagaaaat
      361 cgctacagct actatcgcaa ctgcaggatt cgctacaatc gcaattgcat caggaaatca
      421 agctcatgct tctgagcaag ataactacgg ttataatcca aacgacccaa catcatatag
      481 ctatacttac actattgatg cacaaggtaa ctaccattac acatggaaag gtaactggca
      541 tccaagtcaa ttaaaccaag ataatggcta ctacagctat tactactaca atggctacaa
      601 taactacaac aattacaaca atggttatag ctacaataat tacagccgtt acaacaacta
      661 ctcaaataat aatcaatcat ataactacaa taactataat agttacaaca caaacagcta
      721 ccgtactggt ggtttaggtg caagctacag cacttcaagc aacaatgttc aagtaactac
      781 aactatggct ccatcatcaa atggccgttc aatctcaagt ggttatactt caggacgtaa
      841 cttatacact tctggtcaat gtacatacta cgtatttgat cgtgtaggtg gtaaaatcgg
      901 ttcaacttgg ggcaatgcaa gtaactgggc taacgcagct gcaagagctg gttacacagt
      961 gaacaataca ccaaaagctg gtgcaattat gcaaacaact caaggtgcat acggtcacgt
     1021 tgcatacgtt gaaagtgtta acagcaatgg ttcagtaaga gtttcagaaa tgaactatgg
     1081 ttatggccca ggtgttgtaa cttcacgtac aatctcagct agccaagctg ctggttataa
     1141 cttcattcac taattagtga tggctatgtt ttacgccaac aaggtttcaa aagccttgtt
     1201 ggctttttta atttctatga aaaaaatcga attggtaaaa atctcaaaat ttcgattttc
     1261 accaatccga ttcactctgt aataaagaat aaacaacata cagccattga cacacccaaa
     1321 tttaggcatg tcaaatcggc tatacattga taaattttta gttaaagtac ttacaaatat
     1381 acttgccttc aaattctcaa ttaatttagt cattacaaat catgacataa aacttacatt
     1441 acatatatca acatgttccg ctacaaaaca tattcagtat atgtatagag gatatttatt
     1501 ctaagttaaa accttttcaa attataaccg ctacaattat aaatgattaa agtgtttcaa
     1561 catattatat atattccacg ctcacattaa gttaaacgtg aattcgatga ctttttaata
     1621 cctatccctg taaaaccata gataattgct ataattgcgc ataaataaca tggtactgtc
     1681 cacatgaaaa attctccaac agagacatga agttgattcg tatagtaaat acctgatgtt
     1741 ccccatggta ttaaaggtaa gaccatcgta cttgagtctt caagtgttct tgataaaacg
     1801 gaacgagaga cttggtattt ttcgaacaaa tctttcatta acacaccgac cataataatt
     1861 acaatcgaag caacacctgc agcgaataca agcgcaatac aacaaataac agtaatacat
     1921 attaaacttc ctacagaatg gatgccttta gaaatagtag ttaataagac ttctaaacat
     1981 cctgcttttt ctacaatacc tgcaaatgca tatccgcaaa atatcgttac taatatttgg
     2041 gtcatactca tcataccacc ctgttctaac aagcttttca cactggatga aatatgagac
     2101 tgatgtatca ttgagtcgtt aaaaccacta aatgttgctt tgaaaccatc tgtcattttg
     2161 aaatgatgat taaaagtccc cactataatg gcagaaaagc ttgatattac catagctggc
     2221 actgtagcca ttttaaatag caaacaaaca atgatcacaa ttaagggaac ccagacccat
     2281 atgttaattt gataaatctg tgcaagctct gataacaaag tttgaatctg tttatcattt
     2341 gaatgccctt taaattgaaa tccagcaata aaccatacta ataaacctat gattgatgca
     2401 ggtatcgtcg tccacatcat cgaatgtata tgtttaaata tattaacttt agtaacaagt
     2461 gccgctaaat tagttgtatc tgataatggt gacattttat cgccaaacac agcccctgct
     2521 ataatagcac ccgctgccat ccctggagga atccccaatt gattaccaat agaaataagt
     2581 gcaatccctg cagttgatgc agagccccat gctgtaccag ttgctacaga tgtaacagca
     2641 cttataaaaa aggctgatat taaaaaatag cttggattca ataaatctaa gccataataa
     2701 atcaaggctg gcactgtgcc agaaaacatc caactgccta ctataattcc taccgctagt
     2761 ataattaaaa ttgcaggcat tgcagtattt aaacgttctg caatgccttt ttctaaatct
     2821 tgccatgtta agcctacacg ttttgcaatc catgtggcat atgctgaggc aataattaat
     2881 aatacttgaa ttggaatatc aaaaaacaca aagcctgtta caacaacaat aaccattaca
     2941 atcattgtcg aaattgattc taaaaaggtt ggttttcttt tcatcatcta tcgtcccctt
     3001 cgcttattga tgagaaacct cttttcttca atttattgct tttgcataat gctatatcct
     3061 tttctatctt caagccatca tttattttaa atatttttct aaatcaagct tatctaaacc
     3121 aatttcttca actgttaagc cttcctcaaa gaagtctctc tctaaaatgg ttgatgcaat
     3181 tacaattact gcatctatat ttggtgtcgg tacatcaata acacgaccta agcttgacca
     3241 tagtactaaa ccatatgcaa tatcttcagt taaatatctg ctttctacat gatttggtcc
     3301 tggaatttgt gaaaatactg ggcttgtatt aaacaaacga tttaatggtt catcttcttt
     3361 atcacgttct aaataaccac gttcaatacg tgattcttta gctgttgata attcaaaacc
     3421 taatctacgg cctaaattta aacgttctaa ttcgattgca tgaagtaatc taactgtatg
     3481 tttagtaatt ccttctttat ataaagcgaa ctcgccagca taatcaatgc gaccgacatt
     3541 caataatgtt ggtcctggat gcacttcagg attaccattt tcaagatttg ttttaattaa
     3601 gctttcctct tttactaaat gatcataaat acttgaaact ttgtcataac aatcatttag
     3661 acagcttcta tcatatgttg aaaagaagat acgacgtaca tttagagata aatcaactgc
     3721 tgcattttca aaatcgacac gcgtaccata cgtcaacgta ttagcttccg ctagttgtgg
     3781 ttttgtttca atatgtctat cttctaaaac attcataaaa cgaattgacc ccattgctgc
     3841 agccatgttg aagaatatca actgattatc agttacatgc tctgccatta catcagcata
     3901 atactctatg tatgaagatg gaataatcac ttgaacaatt tcagcatctt ttaaaacata
     3961 ttccatatca tcactaatat cagtgaattt tacgaaacgt tcatcacctt cattattaaa
     4021 atcaaatccg cccttttcga ttgcgttttg aaacttactt atagattgat tacgacaata
     4081 taatttaaca tcgtggcctt tgctcaccat atctactgct gccgtaactg cgccatttcc
     4141 tgatcctaca attgctattt tcataaacaa ccctctttct atctttggtt aaaattaaaa
     4201 acaaattata cgtaagacaa tataaa
//
LOCUS       DAOBBH010000192         4184 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.192, whole genome shotgun sequence.
ACCESSION   DAOBBH010000192 DAOBBH010000000
VERSION     DAOBBH010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.192"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..2068
                     /locus_tag="P6L01_002289"
     CDS             <1..2068
                     /locus_tag="P6L01_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDP3031335.1"
                     /translation="RLKEKSNDEIVQNTINKRINFIFGVIVFIFAVLVLRLGYLQIAQ
                     GSHYKQIIKNDENITVNESVPRGRILDRNGKVLVDNASKMAITYTRGRKTTQSEMLDT
                     AEKLSKLIKMDTKKITERDKKDFWIQLHPKKAKAMMTKEQAMLADGSIKQDQYDKQLL
                     SKIGKSQLDELSSKDLQVLAIFREMNAGTVLDPQMIKNEDVSEKEYAAVSQQLSKLPG
                     VNTSMDWDRKYPYGDTLRGIFGDVSTPAEGIPKELTEHYLSKGYSRNDRVGKSYLEYQ
                     YEDVLRGKKKEMKYTTDKSGKVTSSEVLNPGARGQDLKLTIDIDLQKEVEALLDKQIK
                     KLRSQGAKDMDNAMMVVQNPKNGDILALAGKQINKSGKMTDYDIGTFTSQFAVGSSVK
                     GGTLLAGYQNKAIKVGETMVDEPLHFQGGLTKRSYFNKNGHVSINDKQALMHSSNVYM
                     FKTALKLAGDPYYSGMALPSDISSPAQKLRRGLNQVGLGVKTGIDLPNETRGQIEPLT
                     NNPGNYLDLSIGQYDTYTPLQLSQYVSTIANDGYRIQPHIGLTIHESTNKDEVGPLKK
                     KINGTVLNKVNNTEKEIKQIQEGFKMAFNDKDGTGYVSFKDTVVPTAGKTGTAEVFQN
                     GEPRVNSTYIGYAPIDDPKLAFSIVYTNQPVPPPWLTGGDLGRDVINYYFKQLGKDDK
                     NKDKDK"
     gene            2181..2330
                     /gene="rpmG"
                     /locus_tag="P6L01_002290"
     CDS             2181..2330
                     /gene="rpmG"
                     /locus_tag="P6L01_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDP3031336.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERVEMKKFCSRENKQTL
                     HRETK"
     gene            2539..3078
                     /locus_tag="P6L01_002291"
     CDS             2539..3078
                     /locus_tag="P6L01_002291"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDP3031337.1"
                     /translation="MTKNEIRKYILHKMKNFNKAEKRKADTWLRNQFFATEEYKEANA
                     IALVLSFNHEVDTFSIIEQALMDHKRIFVPKMDYLNHQMTFKEIFNLKDIDVDNKGIY
                     YPTSKGETTNNLDLIVVPGVGFQDDGYRIGYGGGYYDRFLANYQTKTISLLYDFQITS
                     FEPESFDQPVDKLIIYQSA"
     gene            3090..>4184
                     /locus_tag="P6L01_002292"
     CDS             3090..>4184
                     /locus_tag="P6L01_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDP3031338.1"
                     /translation="MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVI
                     FKQHIKSTQEIRFDKAKVLEHKDEIANFISFEPQSFEFYYFTESEFSEEQLNEVSPIR
                     IKFNVIRHTKDLIKHMPNIFLARLISEDNDKKTYMFYKRKVLTDNFLDKYMQKFSPAT
                     YTIIFVNVLIWLCMILYLNNFSDVKLLDVGGLVHFNVVHGEWYRIVTSMFLHFSFEHI
                     LMNMLSLFIFGKIVEAIIGSWRMLTVYFIAGLFGNFVSLSFNTTTISVGASGAIFGLI
                     GSIFAMMYVSKTFNKKMLGQLLIALVILVGVSLFMSNINIVAHIGGFIGGLLITLIGY
                     YYKVNRNIFWILLIGMLVIFIALQIRIFTIK"
BASE COUNT         1601 a          543 c          748 g         1292 t
ORIGIN      
        1 aagactaaaa gaaaaatcaa atgatgaaat cgttcaaaat acaattaaca agagaattaa
       61 ctttatattt ggtgtgattg tatttatttt tgcagtacta gtactacgtt taggttattt
      121 acaaatcgca caaggctcac attataaaca aattataaaa aatgatgaaa acattacagt
      181 gaatgagtct gtgccaagag gtcgtatttt agacagaaat gggaaagttt tagttgataa
      241 tgcttctaaa atggctatta catatactag gggtcgaaaa acaacacaat cggaaatgtt
      301 ggatacggct gaaaagttat caaagctaat caagatggat actaagaaaa ttacagaacg
      361 tgataagaaa gatttctgga ttcagttgca tcctaaaaaa gcaaaagcaa tgatgacaaa
      421 agaacaagct atgttagcag atggaagtat taaacaagat caatatgata aacaactgtt
      481 atcgaaaatc ggaaaatcac aattagatga attgtcttct aaagatttac aagttttagc
      541 tatttttcga gagatgaatg caggaacagt tttagatcca caaatgataa aaaatgaaga
      601 tgtcagtgaa aaagagtatg cagcagtttc tcagcaactt tccaaattac caggtgttaa
      661 cacgtctatg gattgggata gaaaatatcc atatggcgat actttaagag gtatattcgg
      721 agatgtatcg acacctgctg aaggtattcc aaaagaattg acagaacatt acttatccaa
      781 aggatattca cgcaatgatc gtgttggaaa atcttaccta gaatatcaat atgaagatgt
      841 attgcgtggt aagaagaaag aaatgaaata cacaacggac aaatctggta aagttacatc
      901 ttcagaagtg ttaaatcctg gcgctcgcgg tcaagatttg aaattaacga tcgatataga
      961 tcttcaaaaa gaagtagaag cattattaga taaacaaatt aagaagcttc gcagccaagg
     1021 tgcaaaagat atggataatg ctatgatggt tgtacaaaat cctaaaaatg gagacattct
     1081 tgcgcttgcc ggaaagcaga ttaataagag tggtaaaatg actgattatg acattggtac
     1141 gtttacttct caatttgcgg ttggatcttc tgtaaaaggt ggaacattat tagctggtta
     1201 tcagaataaa gctatcaaag ttggagaaac aatggtcgat gaaccattac atttccaagg
     1261 tggtttgaca aaacgatcat acttcaataa aaacgggcat gtatctatta atgataagca
     1321 agctttgatg cattcatcaa acgtatatat gtttaaaaca gcattaaaat tagcgggaga
     1381 cccttattat tctggtatgg ctttaccttc agatataagt tcacctgccc aaaagcttag
     1441 aagaggatta aatcaagtag gcttaggtgt gaaaacaggg atagatttac caaatgaaac
     1501 aagaggtcaa atcgaaccat taacaaataa tccaggtaat tatctagatt tatcaattgg
     1561 tcaatatgat acctatacac cattacaatt atcacaatat gtttcaacta tagcgaatga
     1621 tggttataga atacagccac acattggatt aacgattcat gaatcaacta ataaagatga
     1681 ggttggtcca ctcaagaaga aaattaatgg cactgtcttg aacaaggtta ataatactga
     1741 aaaggaaatc aaacaaattc aagaaggatt caaaatggca tttaatgata aagatggtac
     1801 tggatatgtt agttttaaag atacagtagt acctactgct ggtaaaacgg gtaccgctga
     1861 agtgttccaa aacggagagc caagagttaa ctctacttat ataggatacg cgccaattga
     1921 tgatccaaaa ttagcgtttt caattgtata tacaaatcag cctgtaccac caccatggtt
     1981 aacaggtgga gacttaggta gagatgtaat taactactac tttaagcagt taggtaaaga
     2041 tgataaaaat aaagacaaag acaaataaaa tttaacctga cgattgtgta gcgcatggtt
     2101 gtaaaatttt aactttgcaa atattataga tgttggtata ataataaagt cgtatttaga
     2161 aatgataagg aggaataacc atgcgcgtaa acgtaacttt agcttgtacg gaatgtggtg
     2221 acagaaacta cattacaact aagaacaaaa gaaataatcc agaacgtgtt gaaatgaaga
     2281 aattctgttc acgtgaaaac aaacaaactt tacaccgtga aacaaaataa tcattaaaat
     2341 taaatttaaa ttaacaataa aaaaggtgaa atctcaaaat aagaggtttc acctttttaa
     2401 ttatgcacac gtatgaaatg ataaaagatt ttcaattgaa ataataaatt ttagaattgt
     2461 cgcataattc atcatgacaa cataatgaca tgtattgtta ttaacgatat aatagaattg
     2521 aataaagtgg gtgataatgt gactaaaaat gagattagaa aatacatttt acataaaatg
     2581 aagaatttta ataaagctga aaagcgaaaa gcagacacat ggttaagaaa tcaatttttt
     2641 gcaactgaag aatacaaaga agcaaacgca attgcgctag ttctttcttt taatcatgaa
     2701 gtagatactt tttctattat tgaacaagcc ttaatggatc ataaacgtat ttttgtaccg
     2761 aaaatggatt atttaaatca tcaaatgact tttaaagaaa tatttaatct caaagatatt
     2821 gatgtcgata ataaggggat ttactatcca acttcaaaag gtgaaacaac gaataaccta
     2881 gatttaattg ttgttcctgg tgttggattt caagacgatg gatatagaat tgggtatggt
     2941 ggtggctatt acgacaggtt tttagctaat tatcagacaa agacaataag cttattatac
     3001 gattttcaaa taacatcatt tgaaccggaa tcattcgatc aaccagtcga taaattgatt
     3061 atatatcaat cagcatagtg gaggactaaa tgaacataga caaacaattt tggaaaacaa
     3121 tatattattg gattaggtac ttgaattttg atattgtcag tagggaaaaa gatgaccaag
     3181 aaatttggtt agcccataaa agaaaaaaac aagtagtcat ttttaaacaa catatcaagt
     3241 ctacccaaga aatacgtttt gacaaagcga aagtgcttga acacaaagat gaaatagcaa
     3301 attttatttc tttcgaacca caaagttttg aattttatta ttttacagaa tcggaatttt
     3361 cagaagaaca attaaatgaa gtttcgccaa ttagaattaa attcaatgtt ataagacaca
     3421 caaaagattt gataaagcat atgccgaata tatttttggc tagacttatt tcagaagata
     3481 atgataaaaa gacatatatg ttctataaac gcaaagtatt aaccgataac tttttagata
     3541 aatatatgca gaaattttca ccggcaacat acacaataat atttgtaaat gtcttaatat
     3601 ggttatgtat gattttatat ttaaataatt tttcggatgt aaaattatta gatgttggcg
     3661 ggttagtgca ttttaatgtc gtacatggtg aatggtatcg aattgttaca tcgatgtttt
     3721 tacattttag ttttgaacat atacttatga atatgctttc attatttatt tttggtaaaa
     3781 tagtcgaagc aattattggt tcatggcgga tgttaactgt atactttatt gcagggttgt
     3841 ttggaaactt tgtatcacta tcatttaata cgactacaat ttcagttggg gctagtggtg
     3901 ctatatttgg tctgattgga tcaatttttg cgatgatgta tgtttcaaaa acatttaaca
     3961 aaaaaatgtt aggacagtta ttaattgcat tagtgatatt agttggtgtt tctctgttta
     4021 tgtcaaatat aaatattgtg gcgcatattg gaggattcat tggtggttta ttaataactt
     4081 taattggcta ttactataaa gtgaatcgta atattttttg gattttacta attggtatgc
     4141 ttgttatatt tattgcactt caaattagaa tttttacaat taaa
//
LOCUS       DAOBBH010000193         4157 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.193, whole genome shotgun sequence.
ACCESSION   DAOBBH010000193 DAOBBH010000000
VERSION     DAOBBH010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.193"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            118..1749
                     /locus_tag="P6L01_002293"
     CDS             118..1749
                     /locus_tag="P6L01_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HDP3031339.1"
                     /translation="MKKLTTILFQYKIFPVLMFLVSTGLGILVITQNILIADFLAKII
                     RHQFQGLWIVLFILLGVLLLRATVQFLNQWLGDTLAFKVKHMLRQRVIYKNNGHPIGE
                     QMTILTENIDGLAPFYKSYLPQVFKSMMVPLIIIIAMFFIHFNTALIMLITAPFIPLF
                     YIIFGLKTRDESKDQMTYLNQFSQRFLNIAKGLVTLKLFNRTEQTEKHIYDDSTQFRT
                     LTMRILRSAFLSGLMLEFISMLGIGLVALEATLSLVVFHNIDFKTAAIAIILAPEFYN
                     AIKDLGQAFHTGKQSEGASDVVFEFLEQPNYNNEFLLKYEENQKPFIQLTDISFRYDD
                     SDRLVLNDLNLEIFKGDQIALVGPSGAGKSTLTHLIAGVYQPTIGTISTNQRDLNIGI
                     LSQQPYIFSASIKENITMFKDIENNTIEEVLDEVGLLDKVQSFTKGINTIIGEGGEML
                     SGGQMRRIELCRLLVMKPDLVIFDEPATGLDIQTEHMIQNVLFQHFKDTTMIVIAHRD
                     NTIRHLQRRLYIENGRLIADDRNISVNITENGDDL"
     gene            1746..3419
                     /locus_tag="P6L01_002294"
     CDS             1746..3419
                     /locus_tag="P6L01_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HDP3031340.1"
                     /translation="MKTRLKFQVDKDLLLAIVVGVCGSLVALAMFFLSGYMVTQSALG
                     APLYALMILVVTVKLFGFLRAITRYVERLISHKATFTMLRDIRVQFFGKLVNVIPNVY
                     RKLSSSDLISRMISRVEALQNIYLRVYYPPVVIGLTALVTVIVLAFISIGHALLIMVS
                     MLFTLLIVPWLSSKKARTLKKHAANEQARFLNHFYDYKAGMDELRRFNQINHYRDNLM
                     AKLNHFDKLQLKEQRFLTIYDFILNIIAMLSIFGSLVLGLIQINAGQLNIIYMTSIVL
                     MILTLFEQAVPMTNVAYYKADTDQALHDINEVISVPSTNGKKRLNDKYDATNIYEVKD
                     ASFKYWNQQTYVLSDINFNVNRGEKIAIVGPSGSGKSTLLQIMAGLYQLDSGSVRFEN
                     MDMFEIDDKDKFESLNVLLQSQQLFDGTIRQNLFTDEKDEAVQAIFKQLDLEHLALER
                     QIDLDGHTLSGGEIQRLAITRMLLKDTASTWILDEPTTALDKQNSLKVMDLIEAHAET
                     LIVATHDLTLLSRFETIIVMINGKIVEKGNYQQLLANQGALWNMIQYNA"
     gene            complement(3546..3989)
                     /gene="mgrA"
                     /locus_tag="P6L01_002295"
     CDS             complement(3546..3989)
                     /gene="mgrA"
                     /locus_tag="P6L01_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MgrA"
                     /protein_id="HDP3031341.1"
                     /translation="MSDQHNLKEQLCFSLYNAQRQVNRYYSNKVFKKYNLTYPQFLVL
                     TILWDESPVNVKKVVTELALDTGTVSPLLKRMEQVDLIKRERSEVDQREVFIHLTDKS
                     ETIRPELSNASDKVASASSLSQDEVKELNRLLGKVIHAFDETKEK"
BASE COUNT         1385 a          583 c          734 g         1455 t
ORIGIN      
        1 actagactaa agatgtatat attcattttc ttttacatgt aaaactacaa tattttattg
       61 agctatttaa tttgatttta aggaaaacct tttataatag gtttaggtga tataattgtg
      121 aaaaaattaa caacaatact gtttcaatat aaaatttttc cggtactcat gttcttggtc
      181 agtactggtc tcggcatact cgttataacg caaaatattt taatagcaga ttttttagct
      241 aaaattataa gacatcaatt tcaaggttta tggattgtat tatttatttt attaggtgtt
      301 ttacttttaa gagcaactgt gcaatttcta aatcaatggt taggtgatac attagcattt
      361 aaagttaagc atatgcttag acagcgggtt atttataaaa ataatggtca tccaatcggt
      421 gaacaaatga ctatactcac agaaaacatt gatggtttag caccttttta taagagttat
      481 ttgcctcaag tgttcaaatc aatgatggtt ccgctcatca taatcattgc aatgtttttc
      541 atccatttca ataccgcatt aattatgtta ataactgcac catttattcc tttgttttat
      601 attattttcg gtttgaaaac gcgagatgag tcaaaagatc aaatgactta tttgaatcaa
      661 tttagtcaac ggtttttaaa tattgctaaa ggtttagtga cgttaaagct atttaatcgt
      721 acagagcaaa cagagaagca tatttacgac gatagtactc agtttagaac tttaacaatg
      781 cgcattttac gcagtgcttt tttatcggga ttaatgctcg aatttataag tatgttaggt
      841 attggattgg ttgcattgga agcaacgcta agcttagtag tatttcataa tattgatttt
      901 aaaactgcgg caattgcgat tattttagcg cctgaatttt ataatgcaat taaggactta
      961 gggcaagcgt tccatactgg aaaacaaagt gaaggtgcca gtgacgttgt gtttgagttt
     1021 ttagaacaac cgaactataa taatgaattt ctattaaagt atgaggaaaa ccaaaagcca
     1081 tttattcagt taacagacat atcatttcga tatgatgatt ctgatagatt ggtattaaat
     1141 gatttaaatt tggaaatatt taaaggtgat caaattgcac ttgtaggtcc aagcggggca
     1201 ggtaaatcca ctttgacaca tcttattgca ggtgtttatc agccaacaat aggtactata
     1261 agtacaaacc agcgtgattt aaatatagga atacttagtc aacagccata tattttcagt
     1321 gcttctataa aagagaatat tacgatgttt aaagatatag aaaataatac tattgaagaa
     1381 gtgctagacg aagtaggttt attagacaaa gtgcaatctt tcacaaaagg cattaacaca
     1441 ataataggtg aaggaggcga aatgttatct ggtggacaga tgagacgcat agaactttgc
     1501 cgtcttttag ttatgaagcc agatctcgtt atatttgatg agcctgcaac tggtttagat
     1561 attcaaacag aacacatgat tcagaacgtt ctgtttcaac attttaaaga tacaacgatg
     1621 attgtcattg cacatagaga taatacaatt cgccatttac aacgacgctt gtatatagaa
     1681 aatggaagac tgattgctga tgatcgcaat atttcagtaa atataacaga aaatggtgat
     1741 gacttatgaa aacacgacta aaatttcaag tagataagga tttattgtta gctatagttg
     1801 ttggtgtttg tggaagttta gttgcgctcg ccatgttttt cttaagtggt tatatggtga
     1861 cacaaagtgc acttggtgcg ccactatacg ctctgatgat tttagtcgtt acagtaaaat
     1921 tgtttgggtt tttaagagct attactcgat acgtagagcg ccttatttct cataaagcta
     1981 catttacaat gctacgtgat attcgggtac agtttttcgg taaattagta aatgtcattc
     2041 ctaatgttta ccgtaaactg agttctagtg atttaatttc acgtatgatt agtcgtgttg
     2101 aggcattaca aaatatatat ttacgtgttt attatccacc agtcgttatc ggtttgacag
     2161 cgctagttac agtcatagtt ttggcgttca tttcaatcgg ccatgcgcta ttgattatgg
     2221 ttagcatgtt gtttacttta ctcattgttc cttggttaag ctcaaaaaaa gcacgtactt
     2281 taaagaaaca tgcagctaat gaacaggccc gatttttaaa tcatttttat gattataaag
     2341 ctggtatgga tgaactacgt cgatttaatc aaattaatca ttatcgagat aatttgatgg
     2401 ctaaattaaa tcattttgat aaattacaac ttaaagagca acgcttttta acgatttatg
     2461 attttatatt aaatattatt gctatgcttt cgatttttgg tagtttagtt ctaggattaa
     2521 ttcaaattaa tgcaggccaa ctaaatatta tttatatgac gagtatagtt ttaatgatct
     2581 taactttatt tgaacaagct gtaccaatga caaatgtcgc gtattataaa gcggatactg
     2641 accaagcatt gcacgatatt aatgaagtga tatctgtacc ttctactaat ggaaaaaaac
     2701 gtcttaatga taagtatgat gcaacgaaca tttatgaagt taaggatgct agttttaagt
     2761 attggaatca gcaaacgtat gtgttgtcgg atattaattt taatgttaat agaggcgaaa
     2821 agattgcgat tgtgggtcct tcaggttcag gaaaaagtac attactacaa attatggcag
     2881 ggttatatca attagatagt ggctctgttc gtttcgaaaa tatggatatg tttgaaatag
     2941 atgacaaaga taagtttgaa tcgttaaatg tcttgctaca atctcaacaa ttatttgatg
     3001 gtacaatacg tcaaaattta tttaccgatg aaaaagatga agcggtgcaa gcaatattta
     3061 agcaattaga tttagaacat ttggcactag aacgtcaaat tgacttagat ggtcatacat
     3121 tatctggcgg agaaattcag cgtttagcga ttacgaggat gttattaaaa gatactgcat
     3181 caacatggat tttagatgaa ccaacaactg cattagataa acaaaatagt ttaaaagtta
     3241 tggatttaat tgaagcacat gcagaaacat taattgttgc tacacacgat ttaactttat
     3301 tgtcacgttt tgagaccatc attgtgatga taaatggtaa aatagttgaa aagggaaact
     3361 atcaacaatt actcgctaat caaggtgctt tatggaatat gattcaatat aatgcataaa
     3421 aaaactgctt gataggctaa gaaacctttc aagcagtttt ttaactaaca agcttacgcg
     3481 tgctaattgt ttttctattc ttaataaatt ctaaacatta ctttaattgt catgacaaaa
     3541 gttaattatt tttcctttgt ttcatcaaat gcatgaatga ctttacctaa taagcgatta
     3601 agttctttaa cttcatcttg agataaagaa gaagctgaag cgactttgtc agatgcatta
     3661 cttaattctg gtctaatagt ttcacttttg tcagtcaagt gaataaatac ttcacgttga
     3721 tcgacttcgg aacgttcacg cttaattaag tctacttgtt ccattcgttt taataatggt
     3781 gatactgtac cagtatcgag tgctaattca gttacgactt tcttgacgtt tacaggagat
     3841 tcatcccata aaattgttaa gacaagaaat tgtgggtatg ttagattgta cttcttaaaa
     3901 actttgttag agtagtagcg attaacttgt ctttgagcat tgtacaaact aaagcatagc
     3961 tgttctttta aattatgttg atcagacatt aaagttctcc tccagacata ctatccgttt
     4021 ttttctcttt tcggattggt aatcattaaa aagttgattg tttattaatt cacaactttc
     4081 tttgattcaa tgccatgcta aaattaaagt atgtttaaag tttagaagat atttttgatt
     4141 aaatcaagca aaaagat
//
LOCUS       DAOBBH010000194         4055 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.194, whole genome shotgun sequence.
ACCESSION   DAOBBH010000194 DAOBBH010000000
VERSION     DAOBBH010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.194"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            140..1126
                     /locus_tag="P6L01_002296"
     CDS             140..1126
                     /locus_tag="P6L01_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3031342.1"
                     /translation="MFIIKSMLYRLMQMIVVLFVISTLTFILMKLSPGNPVDKILHLD
                     VAQVSTEQINATKDKLGLNDSLLVQWWHWMNHLLHFNLGKSFESKEPVTQILFNYAPI
                     TLLISFSTLVVSLCISIPLGIIAAKRFHKWTDKVIRVISTLSISLPAFFIGIILLFIV
                     TNLMNIDSVILSQFILPVITLSLGMCAYIIRLVRSNLLMLLQSNIVQASRLRGMNERY
                     ILIHDLLKPTILPIIPLLGISLGSLIGGTVVIENLFDIPGIGYLLMDSIKSRDYPVIQ
                     GCVLFIGFFVVIINTIADLLTLLLDPKQRLQLGNPKNKTNTPLISESSDRHA"
     gene            1119..1949
                     /locus_tag="P6L01_002297"
     CDS             1119..1949
                     /locus_tag="P6L01_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3031343.1"
                     /translation="MHKIFSKNNLIFFVFVAFIFVVIVLQFFVSSENATKVNLSQTFE
                     PISWLHLLGTDDYGRDLFTRIIIGARSTLFVTVLTLIAIVVIGVTLGLFAGYKKGWIE
                     RLVLRFIDVGLSIPEFIIMIALASFFQPSLWNLVISITVIKWMNYTRLTRSIVNSEMN
                     KPYIKMAQLFHVPTRTILIRHLTPKIIPAIIVLMVVDFGKIILYISSLSFIGLGAQPP
                     TPEWGAMLQQGRDFISSHPIMLIAPASVIAITILIFNLTGDALRDRLLKQRGEYDESH
                     "
     gene            1936..2709
                     /locus_tag="P6L01_002298"
     CDS             1936..2709
                     /locus_tag="P6L01_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3031344.1"
                     /translation="MSLIDIQNLTIKNTCEKYLIKGIDLKIFSQQINALIGESGAGKS
                     LIAKALLEYLPFDLSCTYDSYQFDGENISRLSQYYGHTIGYISQNYAESFNDHTKLGK
                     QLTAIYRKHYKSSKEEALSKIDKALSWVNLQSKDILNKYSFQLSGGQLERVYIASVLM
                     LEPKLIIADEPVASLDALNGNQVMDLLQHIVLEHGQTLFIITHNLSHVLKYCQYIYVL
                     KEGQIIERGNINHFKYEHLHPYTERLIKYRTQLKRDYYD"
     gene            2702..3403
                     /locus_tag="P6L01_002299"
     CDS             2702..3403
                     /locus_tag="P6L01_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3031345.1"
                     /translation="MIELKHVTFGYNKKQMVLQDINITIPDGENVGILGESGCGKSTL
                     ASLVLGLFKPVKGEIYLSDNAVLTIFQHPLTSFNPDWTIETSLKEALYYYRGLTDNTA
                     QDQLLLQHLSTFELNAQLLTKLPSEVSGGQLQRFNVMRSLLAQPRVLICDEITSNLDV
                     IAEQNVINILKAQTITNLNHFIVISHDLSVLQRLVNRIIVLKDGMIVDDFAIEELFNV
                     DRHPYTKELVQTFSY"
     gene            3527..4000
                     /locus_tag="P6L01_002300"
     CDS             3527..4000
                     /locus_tag="P6L01_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HDP3031346.1"
                     /translation="MGLLDIASIRSIERGFNYYQSECVINLKSFSETQHEAEVKGSGN
                     KVYRCYIDMEHPRKSKCNCPHADGRRVICKHMIALLFTASPEAANKHIMMLNEVEEDY
                     QLRRNMWIDSLKEMINDMSEEELRDAYLNMLIEHGEMAELFGLDEEEEMFEDEFY"
BASE COUNT         1382 a          556 c          719 g         1398 t
ORIGIN      
        1 taaaaaatta aatatacttc aatttcttta tggataatac tataatgaac gttggttctt
       61 aatatgtaag ttgcatatac atgcgccatt ctcatgtaaa aagaaagtgt tagtaactta
      121 cacaaggagg gggactatta tgttcataat aaagtctatg ttgtatagac tgatgcaaat
      181 gatagttgtt ttatttgtta tttctacact tacattcatt ttaatgaaat tatcaccagg
      241 caatcctgtt gataaaatat tacatcttga tgtagcacaa gtgtcgacgg aacaaataaa
      301 tgcaaccaaa gataaattgg gattaaatga ttctttattg gtacagtggt ggcattggat
      361 gaatcattta ttacatttta atttaggaaa aagttttgaa tcaaaagaac cggtaacaca
      421 gatattattc aactatgcac ccataacatt actcatatca ttttcaacat tagtagtgtc
      481 gttatgcata tctataccgc tcggcattat agcagccaaa cgatttcata agtggacgga
      541 taaagtgatt agagtgatct caacactatc gattagttta ccagcgttct ttatcggtat
      601 tattttatta tttattgtca caaatttaat gaatatagat agcgttatac taagtcagtt
      661 tatattacct gtaatcacgc tatctttagg tatgtgtgca tacatcattc gtttagtgcg
      721 ttctaattta ttgatgttat tgcaaagtaa tatcgtacaa gcatcaagat tacgcggtat
      781 gaatgaacgt tatattttaa ttcatgattt actaaaacca acaattttgc cgattatccc
      841 attactaggg atttcacttg gcagtctaat aggtggtact gtagtgattg aaaatttatt
      901 tgatatacct ggtattggtt atctattaat ggatagtatt aaatctcgag attatcctgt
      961 tattcaagga tgcgtgttat ttattggctt cttcgttgtt attatcaata cgattgctga
     1021 tttattaacg ttattacttg atccgaagca gcgtttacaa ttaggaaatc caaaaaacaa
     1081 aaccaataca ccattgatat cagaaagtag tgaccgtcat gcataaaata ttttcaaaga
     1141 ataacctgat attttttgta ttcgttgcat ttatttttgt ggtaattgta ctgcaattct
     1201 ttgtcagtag tgaaaatgca accaaagtca atttatcaca aacttttgaa cctattagtt
     1261 ggttgcattt attaggaact gatgattatg ggagagattt atttacccga attattatcg
     1321 gtgcacgttc aacattgttt gttactgttt taacattaat agctatcgtt gtcataggtg
     1381 ttacactagg tctatttgcc ggatataaaa aagggtggat tgaacgatta gtgttaaggt
     1441 ttattgatgt tggtctaagt attccagaat ttatcatcat gattgcttta gcaagctttt
     1501 ttcaaccatc tttgtggaat ttagttatct caattacagt aataaaatgg atgaattaca
     1561 caaggttgac tagaagtata gttaatagcg aaatgaataa gccttatata aaaatggcac
     1621 aattatttca tgtacctaca agaacaatat taatacgtca tttaacacct aaaattatac
     1681 cggctattat cgttttgatg gtcgttgatt tcggtaaaat cattttatat ataagttcac
     1741 tatcatttat tgggttaggt gcacaaccgc caacaccaga gtggggcgct atgttgcaac
     1801 aaggtcgtga ttttatttcg tctcatccaa ttatgttgat tgcacctgct tcagtcattg
     1861 ctataactat tttaattttt aatttaaccg gagatgcgct aagagataga ttgctaaaac
     1921 aacgaggtga atatgatgag tctcattgat atacaaaatt taacaataaa gaatacttgt
     1981 gagaaatatc ttattaaagg gattgatttg aaaattttta gtcaacagat taatgccttg
     2041 attggagaga gcggcgctgg gaaaagtttg attgctaaag ccttacttga atatttacca
     2101 tttgatttaa gctgcacgta tgattcgtac caatttgatg gagaaaatat tagtagattg
     2161 agtcaatatt atggtcatac aattggttat atttctcaaa attatgcaga aagttttaac
     2221 gaccatacta aattaggtaa acagttaact gcgatttatc gtaagcatta taaaagtagt
     2281 aaagaagagg ctttgtcaaa aattgacaag gctttgtcgt gggttaattt acaaagcaaa
     2341 gatatattaa ataaatatag tttccaactt tctgggggcc aacttgaacg cgtttacata
     2401 gcaagcgttc tcatgttgga gcctaaatta atcattgcag acgaaccagt tgcatcattg
     2461 gatgctttga acggtaatca agtgatggat ttattacagc atattgtatt agaacatggt
     2521 caaacattat ttattatcac acataactta agtcatgtat tgaaatattg tcagtacatt
     2581 tatgttttaa aagaaggtca aatcattgaa cgaggtaata ttaatcattt caagtatgag
     2641 catttgcatc cgtatactga acgtctaatt aaatatagaa cacaattaaa gagggattac
     2701 tatgattgag ttaaaacatg tgacttttgg ttataataaa aagcagatgg tgctacaaga
     2761 tatcaatatt actatacctg atggagaaaa tgttggtatt ttaggcgaaa gtggctgtgg
     2821 taaaagtacg ctcgcttcat tggttcttgg cttgtttaaa cctgttaaag gagaaattta
     2881 cttaagtgat aatgctgtgt taacgatttt ccaacaccct ttaactagct ttaaccctga
     2941 ttggacgatt gagacctcat taaaagaagc gttatattat tacagaggcc taactgataa
     3001 tactgctcag gatcaattat tattacaaca tttatctact tttgagttaa acgcgcaatt
     3061 attgactaaa ttaccaagcg aagtgagtgg cggacaatta caaagattta atgtcatgcg
     3121 ttcgttatta gcacagcctc gcgttttaat atgtgatgag ataacttcaa atttagatgt
     3181 tatagctgaa caaaatgtaa tcaatatatt aaaagcgcaa acgattacga acttaaatca
     3241 ttttatcgtt atttctcatg atttatccgt gttacaacgc ttagttaata gaattatcgt
     3301 tcttaaggat ggcatgatag tcgatgattt tgcaatagag gaattattta atgttgatag
     3361 acacccttat acaaaagaat tagtgcaaac attttcatat tagctattta agaatgcgat
     3421 aattctagac ttgttataaa atatagatga atcaagtata ttaatctaga cacttatcga
     3481 ttttattttc tttatttaaa aataataata aaaaggagta tcattaatgg gattacttga
     3541 tattgcaagt attcgttcta tagaaagagg ctttaattat tatcaaagtg aatgcgtcat
     3601 taacttaaaa tcattttcag aaacgcagca tgaggctgaa gtaaagggca gtggcaacaa
     3661 agtatatcgt tgttatattg atatggaaca tcctagaaaa tccaaatgta attgtcctca
     3721 tgctgatgga agacgagtga tatgtaaaca tatgattgca ttactcttta cagctagtcc
     3781 agaagcagca aataaacata taatgatgtt aaacgaagtt gaagaagact atcaattacg
     3841 cagaaatatg tggattgata gtcttaaaga aatgattaat gatatgagtg aagaagaact
     3901 ccgcgatgca tatttaaaca tgttaattga acatggagaa atggcagaat tatttggatt
     3961 agatgaagag gaagaaatgt tcgaggacga attttattaa aatagcccct atcgattgat
     4021 aatgattatc atttgatagg ggtgctttta tttat
//
LOCUS       DAOBBH010000195         4042 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.195, whole genome shotgun sequence.
ACCESSION   DAOBBH010000195 DAOBBH010000000
VERSION     DAOBBH010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.195"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(83..640)
                     /gene="efp"
                     /locus_tag="P6L01_002301"
     CDS             complement(83..640)
                     /gene="efp"
                     /locus_tag="P6L01_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDP3031347.1"
                     /translation="MISVNDFKTGLTISVDNAIWKVIDFQHVKPGKGSAFVRSKLRNL
                     RTGAIQEKTFRAGEKVEPAMIENRRMQYLYADGDNHVFMDNESFEQTELSSDYLKEEL
                     NYLKEGMEVQIQTYEGETIGVELPKTVELTVTETEPGIKGDTATGATKSATVETGYIL
                     NVPLFVNEGDVLIINTGDGSYISRG"
     gene            complement(666..1727)
                     /locus_tag="P6L01_002302"
     CDS             complement(666..1727)
                     /locus_tag="P6L01_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDP3031348.1"
                     /translation="MSRITQVHRILEQKHLDAIIILSDYNRRYLSGFTGTSGALIISK
                     DKQYLITDFRYIDQATKQAPNYEIINRKSTIIGEIKELLHQENFENIGFEGHHVSYDT
                     YLELNKSRISLISISNTVDKIRDVKDVDEIALIQKAANIVDETYEYILTVVKAGMTEK
                     ELKAILESKMLELGADGPSFDTIVASGHRGALPHGVASDKIIEKGDMITLDFGAYYNG
                     YCSDITRTFAIGEPDPKLKEIYQIVLESQMKAINEIRPGMTGAEADAISRNYLESKGY
                     GKEFGHSLGHGIGLEIHEGPMLARTIQDKLQVNNCVTVEPGVYIEGLGGIRIEDDILI
                     TENGCQVFTKCTKDLIVLT"
     gene            1832..2413
                     /locus_tag="P6L01_002303"
     CDS             1832..2413
                     /locus_tag="P6L01_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031349.1"
                     /translation="MKKLVSIVGATLLLAGCGSQNLAPLEEKTTDLREDNHQLKLDIQ
                     ELNQQISDSKSKIKGLEKDKENSKKTASNNTKIKLMNVTSTYYDKVAKALKSYNDIEK
                     DVSKNKGDKNVQSKLNQISNDIQSAHTSYKDAIDGLSLSDDDKKTSKNIDKLNSDLNH
                     AFDDIKNGYQNKDKKQLTKGQQALSKLNLNAKS"
     gene            2427..2645
                     /locus_tag="P6L01_002304"
     CDS             2427..2645
                     /locus_tag="P6L01_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031350.1"
                     /translation="MRNIIFYLVLIIAAIGLVMNLDAFIFSIVRMLISFAVIAGIIYL
                     IYYFFILTEDQRKYRKAMRKYKRNQRRK"
     gene            complement(2709..3539)
                     /locus_tag="P6L01_002305"
     CDS             complement(2709..3539)
                     /locus_tag="P6L01_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDP3031351.1"
                     /translation="MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWN
                     PATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMP
                     STVTEAYRVISQGLLEGFKNLGFDTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVV
                     EGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAI
                     NDISDEHITISQMEEAFEKGFKKGLNIELKPLELTEAQLAEVEELTEKYRSDEWMFRK
                     "
     gene            3697..>4042
                     /locus_tag="P6L01_002306"
     CDS             3697..>4042
                     /locus_tag="P6L01_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDP3031352.1"
                     /translation="MSASLYIAIILVIAIIAYMIVQQILNKRAVKELDQNEFHNGIRK
                     AQVIDVREKVDYDYGHINGSRNIPMTMFRQRFQGLRKDQPVYLCDANGIASYRAARIL
                     KKNGYTDIYMLKG"
BASE COUNT         1356 a          706 c          567 g         1413 t
ORIGIN      
        1 caatagcttt atgagactta tttagaatat cttacgttaa atcagtatag tgaatacaag
       61 ctatttgtta acaaattaga gattatcctc ttgaaatgta gcttccatca ccagtgttga
      121 taattaaaac gtcaccttcg tttacaaata aaggtacatt taatatataa ccagtttcaa
      181 cagttgccga tttagtggca ccagttgcag tatcaccttt aataccaggt tctgtttcag
      241 ttactgttaa ttcaacagtt ttaggtaatt caacaccgat agtttcacct tcgtatgttt
      301 gaatttgtac ttccatacct tcttttaagt aattcaattc ttcttttaag taatcacttg
      361 aaagttctgt ttgttcaaag ctttcattat ccataaatac atgattatct ccgtcagcat
      421 ataaatattg catgcgacga ttttcaatca ttgctggttc aactttttca ccagctctaa
      481 acgttttctc ttgaattgca ccagttctta aattacgtaa ttttgaacga acgaatgctg
      541 aacctttacc aggctttaca tgttggaagt ctataacttt ccaaatagcg ttatcaacag
      601 aaattgttaa acctgtttta aaatcattaa ccgaaatcat tcagtttcct cctcatttta
      661 cacgcttatg ttaaaactat aaggtctttt gtgcatttag taaagacttg acaaccattt
      721 tctgtaatta aaatatcatc ttctattctt ataccgccca aaccttctat ataaacacca
      781 ggttctactg taacacagtt gttaacttga agtttatctt gtatcgtacg agccagcatt
      841 ggcccttcat ggatttctaa accaatacca tgtcctagtg aatgtccaaa ttcttttcca
      901 tacccttttg actctaaata gtttcttgaa atggcatcag cttctgcacc agtcatgcca
      961 ggtctaatct cattaattgc tttcatttga gattcaagta ctatttgata tatttctttc
     1021 agtttaggat ctggttctcc aatagcaaat gttctagtaa tatctgaaca atagccgtta
     1081 taatacgcgc caaaatctaa tgtaatcatg tcgccttttt caataatttt atcacttgca
     1141 acaccatgtg gtaatgcacc tctatgacca gatgctacaa tcgtatcaaa tgatggtcca
     1201 tctgctccta attctagcat tttactttct aatattgcct ttaattcttt ttcagtcata
     1261 cctgctttta caacagttaa aatatattca tatgtttcat caacaatatt agctgctttt
     1321 tgaattaaag caatttcgtc aacatctttg acgtctctaa ttttatctac agtattagaa
     1381 atgcttatta atgatatacg gcttttattt aattcaaggt atgtatcata acttacatga
     1441 tgcccctcaa aacctatatt ttcaaaattt tcttggtgta gcaattcttt aatctcacca
     1501 ataatagtag atttacgatt aataatttca taatttggcg cctgcttagt tgcttgatca
     1561 atatatctaa agtctgttat caaatattgt ttatctttag atatgataag tgctccactg
     1621 gtaccagtaa aacctgataa atatcttcta ttgtaatccg aaagaatgat aatcgcatct
     1681 aaatgttttt gttctaaaat acgatgcact tgtgttattc tgctcatctt attacctcct
     1741 agtgaaacgt ttgtgcattt caactttata cattaaaata atatcataat aaggataaaa
     1801 aataatagat attgatttta gggagatagt aatgaaaaaa ttggtttcaa ttgttggcgc
     1861 aacattattg ttagctggat gtggatcaca aaatttagca ccattagaag aaaaaacaac
     1921 agatttaaga gaagataatc atcaactcaa actagatatt caagaactta atcaacaaat
     1981 tagtgattct aaatctaaaa ttaaagggct tgaaaaggat aaagaaaaca gtaaaaaaac
     2041 tgcatctaat aatacgaaaa ttaaattgat gaatgttaca tcaacatact acgacaaagt
     2101 tgctaaagct ttgaaatcct ataacgatat tgagaaagat gtaagtaaaa acaaaggcga
     2161 taagaatgtt caatcgaaat taaatcaaat ttctaatgat attcaaagtg ctcacacttc
     2221 atacaaagat gctatcgatg gtttatcact tagtgatgat gataaaaaaa cgtctaaaaa
     2281 tatcgataaa ttaaactctg atttgaatca tgcatttgat gatattaaaa atggctatca
     2341 aaataaagat aaaaaacaac ttacaaaagg acaacaagcg ttgtcaaaat taaacttaaa
     2401 tgcaaaatca tgataggagt cttttaatgc gtaatataat attttatctt gtacttatta
     2461 ttgctgcgat tggattagta atgaatctag atgcctttat tttttcaatc gtcagaatgt
     2521 taatcagctt tgctgtaata gctggtatta tttatctgat ttattatttc ttcatcttaa
     2581 ctgaagacca acgcaaatat cgcaaagcaa tgcgtaagta taaaagaaac caaagaagaa
     2641 aatagataaa aaaacggaag cacttgtagg taaaatagtc tacgtgcttc cattttttat
     2701 tctaaaaact actttctaaa catccattca tctgaacgat atttttcagt taattcttcc
     2761 acttctgcca attgagcttc tgttaattca agtggcttta attctatatt taaacctttc
     2821 ttaaaacctt tctcgaaagc ttcttccatt tgactaatag taatgtgttc atctgaaata
     2881 tcattgatgg caactgcttt ttcaacgaat gcctctttca tttttaattt taatctttca
     2941 tttttataaa taaacatatc aaacagttca tcaatatcaa tatcttgtaa aatcgaaccg
     3001 tgttggagga ttacgccctt ttgtctcgtt tgagcactcc cagcaatctt acggccttca
     3061 acaactagct cataccaact tggtgcatca aaacacactg aacttcgagg ttgttttaat
     3121 ttttgacgct cttcaggcgt tttaggtacc gcaaaataag tatcaaatcc taagttttta
     3181 aatccttcta ataatccttg tgaaatcact ctgtacgctt ctgtaactgt agaaggcata
     3241 ttcggatgcg attcaggcac aatcacactg taagttaact ctttatcatg tagcacccca
     3301 cggccaccag tttgacgcct tacgagacca aaacctttct ctttaacctt atcaatatca
     3361 atttcttttt gtagcctttg gaaataccct attgataatg ttgcaggatt ccatgtgtaa
     3421 aaacgtataa ctggatcaat ttcacctcta gagacaaaat ttaataacgc ttcatccatt
     3481 gccatattat aatatgggtc tttacttcct gtattaataa aattccaagt ttcagtcata
     3541 ttcaagaact cctatcagca aaatgtataa tatttgattc gctaattaat caatttaact
     3601 aaatgaataa taattgcaat tctttagtga aatattttga taatttgacc taacagtctt
     3661 ataattatat tatcgtttaa ttagggagga tgcaagatga gtgctagttt gtacatcgca
     3721 ataattttag ttatagcaat tattgcttat atgattgttc aacaaattct taacaagcga
     3781 gctgttaaag aattagatca aaatgaattc cataatggga ttagaaaagc tcaagtcatc
     3841 gatgttagag agaaagttga ctatgactac ggtcacatta atgggtctcg caatattcct
     3901 atgacaatgt tcaggcaacg attccaagga ttaagaaaag atcaaccggt atacttatgt
     3961 gatgccaatg ggattgctag ctatagagcc gctcgtattt tgaaaaagaa tggatataca
     4021 gatatctata tgttaaaagg cg
//
LOCUS       DAOBBH010000196         4019 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.196, whole genome shotgun sequence.
ACCESSION   DAOBBH010000196 DAOBBH010000000
VERSION     DAOBBH010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.196"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(203..1321)
                     /gene="mnmA"
                     /locus_tag="P6L01_002307"
     CDS             complement(203..1321)
                     /gene="mnmA"
                     /locus_tag="P6L01_002307"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDP3031353.1"
                     /translation="MSNKDIRVVVGMSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTD
                     ENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMC
                     NKEIKFKAFLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQ
                     QQLSKVMFPIGDIEKSEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPAQP
                     GDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHD
                     ALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEP
                     VRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV"
     gene            complement(1322..2464)
                     /locus_tag="P6L01_002308"
     CDS             complement(1322..2464)
                     /locus_tag="P6L01_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDP3031354.1"
                     /translation="MEIYADYAATTPVKPEVVDAMMMIYNSHYGNPSSIHAKGRDARK
                     YLDESRRQIAQLLGADTHEIIFTSGATESNNTAIKGIVKANEQLGNHIITSKIEHHSV
                     LHVFEQLEREGFDVTYLDVDDTGAIDLDQLEETITDKTILVSIMFVNNEVGTVQQIYD
                     IQDIIAETNAYFHVDAVQAIGHLDVKFDEFEIDAMSITAHKFGGPKGVGALLVKDHVT
                     LDYPQLGGEQELKRRAGTENLAQIVGMAKALQLAEKNRDDNNIHLMNLKEQFLVKLQE
                     RAIPFELNGSMTDATGHIVNLYFPFVEVEMMLTLLDMAQIYVSSGSACTAGSTQPSHV
                     LDAMFEDEERSNHSIRFSFNELTTENEINAIVAEIHKIYFKFKEES"
     gene            2776..3789
                     /locus_tag="P6L01_002309"
     CDS             2776..3789
                     /locus_tag="P6L01_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDP3031355.1"
                     /translation="MAIKYSALNLVPIREGDDERTAINDMVKLAQHLDELSYERYWIA
                     EHHNAPNLVSSATALLIQHTLEHTKHIRVGSGGIMLPNHAPLIVAEQFGTMATLFPNR
                     VDLGLGRAPGTDMMTASALRRDQHDGVYKFPEEVSLLQQYFGPAHQQAYVRAYPAVGK
                     NVPLYILGSSTDSAHLAARKGLPYVFAGHFAPQQMKEAIEIYKTLFEPSDVLDEPYVI
                     VCLNTIVAENDDEAQYLASSMAQVMVSITRGRMQPVQPPTHDLQNILTPREYAMAMER
                     QKISLIGSENTVQQKIQDFMETYGEVNEIMAISYIYDKDMQLDSYRRFKNVINQINEK
                     NTL"
BASE COUNT         1269 a          763 c          604 g         1383 t
ORIGIN      
        1 ttggggcccc gtccccaact tccattgcct gtagaatttc tttacgaaat tctctatgtt
       61 ggggccccgc caatataaca ttgtagagcc taggacattg tgatgtccca gactctatca
      121 tcatgaatta ttctcatcaa aaactgtctt tcgtcatttt cgacgttgaa acttcaaata
      181 agtaatttat tgttgccatt gtttatacaa cataatttaa ttgaccttca tttttgaaca
      241 catcgtcaat tgttgcacca ccaagacaca catcaccttg ataaaaaaca actgcttgtc
      301 caggtgtgat tgctcttact ggctcagcaa aagtaacacg tagtgcatgg tcgttttcac
      361 gtttcacaaa aactttcgta tctttttggc gatatctaaa tttagctgta cattcaaaac
      421 cttgatctaa gtcattatct tctggattta caaatgaata gtctgaagca attaagtaat
      481 cactgtataa tgcatcgtga tggaatcctt gttctacata taaaacatta tcttttaggt
      541 ttttaccgac aacaaaccaa ggatcgccat ctccacctat acctaatcca tgtctttgtc
      601 ctattgtgta atacatcaaa ccactatgtt tacccatttt cttaccatca agtgttatca
      661 tatcacccgg ttgtgcaggt aaatattgtg ataaaaatgt tttaaagttt ttttcgccga
      721 taaaacaaat gcctgtagaa tcttttttct tagcagtaac aagtccttgt tcttcagcaa
      781 ttcgacgcac ttcactcttt tcgatgtcgc caattgggaa catcactttt gaaagttgtt
      841 gttgagataa ttgattcaag aagtatgttt gatctttatt attatctaca ccacgtaaca
      901 tttcaacatg accatcttca tgacgatgta tgcgtgcgta atgtcctgtt gctacataat
      961 ctgcacctaa attcatcgca tgatctaaaa aggctttaaa cttaatttct ttattacaca
     1021 taacgtctgg atttggagta cgaccttttt tgtattcatc taagaaatac gtaaagactt
     1081 tatcccaata ttctttttca aaattaacag cgtaatatgg aatgccaatt tgattacaca
     1141 cttcaataac atcgttgtaa tcttcagttg cagtacatac gccattttcg tcagtgtcat
     1201 cccagttttt cataaatatg ccaatgacat cataaccttg ttcttttaag acgtgggctg
     1261 ttacagaact atctacaccg cctgacatac caacgacaac acgtatatct ttatttgaca
     1321 attatgactc ctccttaaat ttaaaatata ttttatgaat ttcagctaca attgcattaa
     1381 tttcattttc agtagtcaat tcgttaaaac taaatcgaat cgaatgattt gatcgctcct
     1441 catcttcgaa cattgcatct aaaacatgcg acggttgtgt agagcctgct gtacatgcag
     1501 atccagacga cacatagatt tgtgccatat ccaacaatgt taacatcatt tcaacttcaa
     1561 caaacggaaa atatagattt acaatatggc ctgtagcatc cgtcattgaa ccatttaatt
     1621 caaatggaat cgctctttct tgtaatttaa ctaaaaattg ttcttttaaa ttcattaaat
     1681 gaatattgtt atcgtctcga ttcttttctg ctaattgtaa tgctttagcc atcccgacaa
     1741 tttgtgcaag attttcagta cctgcacggc gtttcaattc ttgttcaccg ccaagttgag
     1801 gataatctaa tgtgacatgg tctttaacta ataatgcacc gacacctttt ggtccgccaa
     1861 acttatgtgc agtaatactc attgcgtcga tctcaaattc gtcaaactta acatcaagat
     1921 gtccaattgc ttgaaccgca tcaacatgga aatatgcatt tgtctcagca ataatatctt
     1981 gaatatcata aatttgttgc actgtgccaa cttcattatt tacaaacata atagatacta
     2041 aaatcgtctt atctgtaatt gtttcttcaa gttgatctaa atcaatagca cctgtatcat
     2101 caacatctag atatgttaca tcaaaacctt ctcgctctaa ttgttcaaaa acatgtaaca
     2161 cagaatgatg ttcaatcttc gatgtgataa tgtgattacc caattgttca tttgctttta
     2221 ctatgccttt aattgccgta ttattcgatt ctgttgcacc actcgtaaat ataatttcat
     2281 gtgtatctgc accaagtaat tgtgcaattt gacgtcttga ctcatctaaa tatttacgcg
     2341 catctcttcc cttagcatgt attgatgatg gattaccata atgcgaattg taaatcatca
     2401 tcatcgcatc tactacttca ggttttactg gtgtggtcgc agcataatct gcataaattt
     2461 ccatgtttgg acactcctca caattttatc aatgttccaa taatagcacc ttacatacta
     2521 tttttctact tttctgttta actttattta taatgttttt aattatattt taccattttc
     2581 tacacatgct tttcgatagg cttttttaag tttatcgctt tattcttgtc ttttttataa
     2641 attttagtat ttgcagatat tttttcattt gtaaaatgta acgtactatt attttggtta
     2701 tgagcaattt aatatttatc tggttattcg attggtatac ttcttatatc ataaaaaagg
     2761 aaggacgata taaaaatggc gattaaatat tcagcattaa accttgtccc tattcgagaa
     2821 ggtgacgatg aacgaacagc aattaatgat atggttaagc tcgcacaaca tttagatgaa
     2881 ttatcgtatg aaagatattg gattgctgaa caccataacg ctcccaacct agtaagttca
     2941 gcaactgctt tattgattca acatacgtta gaacatacga aacacatacg tgtaggttct
     3001 ggaggaatca tgttacctaa ccatgctcca ttaatcgttg cggaacaatt cggcacgatg
     3061 gcaacattat ttccaaatcg tgtcgattta ggattaggac gtgcacctgg aacagatatg
     3121 atgaccgcaa gtgcattaag acgagatcaa catgatggtg tttataaatt tccagaagaa
     3181 gtttcattat tacaacaata tttcggccca gctcaccaac aagcatatgt tcgtgcttat
     3241 ccagcagtag gtaaaaatgt gcctttatac attcttggtt cttcaacaga ttctgcacat
     3301 ttagctgctc gcaaagggct tccatatgtg ttcgctggac attttgcacc tcaacaaatg
     3361 aaagaagcta tcgaaattta caaaacgtta tttgaacctt ctgatgtatt agacgaacct
     3421 tatgttattg tatgtttaaa tacaatcgtt gctgaaaatg atgacgaagc acaatattta
     3481 gcttcatcta tggcacaagt aatggttagt atcactcgtg gcagaatgca gcccgttcaa
     3541 ccgccaacac atgatctaca aaatatatta acgccgagag aatatgcgat ggctatggaa
     3601 agacagaaaa tatcattaat aggttcagaa aatactgttc aacaaaaaat tcaagatttt
     3661 atggaaactt atggtgaagt caacgaaatt atggcaataa gttatattta tgataaagat
     3721 atgcaattag actcttatcg tcggttcaag aatgttataa atcagataaa tgaaaaaaac
     3781 actttataat gtgataaata aactaagtga aagtatgtat ccataatatt aataaaaata
     3841 aacagtaaca gcattttgaa taaaagatgt ctttattgtt caatcatttg ttttagtaat
     3901 gattcaaatt cacttaaaat tctaaagcaa atatgaaagc gccccttcaa ctttacactg
     3961 tgttagtgtt tatttgatgg ggcgctttca aaatattgaa aagcatatcc aaaatttaa
//
LOCUS       DAOBBH010000197         4000 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.197, whole genome shotgun sequence.
ACCESSION   DAOBBH010000197 DAOBBH010000000
VERSION     DAOBBH010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.197"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            86..1039
                     /locus_tag="P6L01_002310"
     CDS             86..1039
                     /locus_tag="P6L01_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031356.1"
                     /translation="MKKTLGCLLLIMLLVVAGCSFGGNHKLSSKKSEESKQETVKKES
                     EEEKDPDLEKYEEIEKKMKGIKDAPSLDKLDPLMTEKSFTNSKGIQGWKDYKELMGKV
                     ELADYRFTKDSKGSSIKDVDAFFKGKKGIKRKVIETHDDVKQVDYWYVDPDGKKIGNS
                     NTPVFYAEIMTKYKDGKLVYASVEPGSYVIHKDDAIKYDDYSKLKKLSQLTKLDHPKP
                     VPYSVAQIKSFGVPLTSVSFMTHGSKDTKDEVLPALAYFTFSPKNYEDKSNPDPKVLN
                     LVHMDFLNASSDFGNAHFVVLSKYIKEYESNYETASDDSLK"
     gene            complement(1202..1405)
                     /locus_tag="P6L01_002311"
     CDS             complement(1202..1405)
                     /locus_tag="P6L01_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HDP3031357.1"
                     /translation="MQVNKVIYILLALFLGSFGIHKFYAGKNMQGILHLIFCWTGIPH
                     ILAIISAVITVFKPADEQGNVTL"
     gene            1704..2192
                     /locus_tag="P6L01_002312"
     CDS             1704..2192
                     /locus_tag="P6L01_002312"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDP3031358.1"
                     /translation="MAIKKLVPASHPILTKKAQAVKTFDDSLKRLLQDLEDTMYAQEA
                     AGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIISQSNETITDLEGSITLPDVYGEVTR
                     SKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
                     ND"
     gene            2185..3120
                     /locus_tag="P6L01_002313"
     CDS             2185..3120
                     /locus_tag="P6L01_002313"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDP3031359.1"
                     /translation="MTKIIFMGTPDFSTTVLEMLIAEHDVIAVVTQPDRPVGRKRVMT
                     PPPVKKVAMKYDLPVYQPEKLSGSEELEQLLQLDVDLIVTAAFGQLLPESLLALPKLG
                     AINVHASLLPKYRGGAPIHQAIIDGEQETGITIMYMVKKLDAGNIISQQAIKIEENDN
                     VGTMHDKLSVLGADLLKETLPSIIEGTNESVPQDDTQATFASNIRREDERISWNKPGR
                     QVFNQIRGLSPWPVAYTTMDDTNLKIYDAELVETNKINEPGTIIETTKKAIIVATNDN
                     EAVAIKDMQLAGKKRMLAANYLSGAQNTLVGKKLI"
     gene            3117..>4000
                     /gene="rsmB"
                     /locus_tag="P6L01_002314"
     CDS             3117..>4000
                     /gene="rsmB"
                     /locus_tag="P6L01_002314"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDP3031360.1"
                     /translation="MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNAMDKALF
                     TEIVYGTVKRKYTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVE
                     IAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATHYG
                     LEETETILQSFLETTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQP
                     IIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQV
                     DASDIHDHKIDLINFNIK"
BASE COUNT         1506 a          560 c          721 g         1213 t
ORIGIN      
        1 aatgtaaaat atcaatataa ataagttttt taaatttatg tatgtttatt tgattcaaac
       61 aaaataactt aagaggagaa agtttatgaa aaagacactg ggatgtttac ttttaattat
      121 gcttttagtc gtagcaggtt gttcttttgg tgggaatcat aaattatcat caaagaaatc
      181 agaagaatca aaacaagaaa ctgtaaaaaa agaatcggaa gaagagaaag atccagattt
      241 agagaaatat gaagaaatag agaagaaaat gaaaggaatt aaagatgcgc catctcttga
      301 taagttggat ccattaatga cagaaaagtc gtttacgaat agtaaaggga ttcaaggatg
      361 gaaagattac aaagaattaa tgggtaaagt ggaacttgca gattatagat ttactaaaga
      421 ttcaaaagga tcttcaataa aagatgttga tgcattcttt aaaggtaaga aaggtataaa
      481 aaggaaagtg attgaaacac acgatgatgt aaaacaagtt gattattggt atgtagatcc
      541 agatggaaag aaaattggca attcaaacac acctgttttt tacgcagaaa ttatgacaaa
      601 atataaagat ggaaagttag tttatgcatc agtcgaacca ggatcttacg taatacataa
      661 agatgatgca attaaatatg acgattattc taagttaaaa aaattaagtc agctaactaa
      721 acttgatcat ccaaaaccag ttccatatag cgtagctcaa atcaaatctt tcggagtacc
      781 tttaacaagc gtttcattta tgacacatgg atcaaaggat actaaagatg aagtgttgcc
      841 ggcattggcc tatttcactt tttcaccaaa aaattatgaa gacaagtcta atccagatcc
      901 aaaagtttta aatttagtac atatggattt cttaaatgca tctagtgatt ttggtaacgc
      961 acattttgtt gttttaagta aatatattaa agagtatgaa tcaaactatg aaacagcgtc
     1021 agatgattct ttaaaatagt atttactgtg tgaaaaataa atagtgtact acattaaata
     1081 atcgcaataa taatcccgat aaacaatcag cattactgct tatcatatag agttcgtaat
     1141 aactataact ctatgattcg caaataataa atgattgtca tcgggattta tttttatcaa
     1201 tttataaagt gacattacct tgttcatcag caggtttgaa aacagtaatc actgcactaa
     1261 taattgctaa aatgtgtggg atacctgtcc aacagaatat taagtgtaga ataccttgca
     1321 tattcttgcc ggcataaaat ttatgaatac caaaactacc taagaacaat gctaataaaa
     1381 tataaataac tttgtttact tgcatttctt tccctccagt tgaattgctt ataatgtcat
     1441 tagcttctct ttttattata cccactttta gttcaaacat tctagtttaa gcattcccaa
     1501 tcatctaaat ttcagttatt cattccttac aataaattta ggattacact tcagttgcat
     1561 tgtattattt tacgtgtgaa atatacgtaa tgaatcacat gacaatcttc aaattgaaaa
     1621 atatacattc tatgatgtaa ggtcgcattt ttaatatatt tacgttataa tagtttgatg
     1681 aattttattg aggagtaaga gttatggcga ttaaaaagtt agtaccagca tcgcatccta
     1741 ttttaacgaa aaaagcgcaa gcagttaaaa catttgatga ttcgttaaaa agattattac
     1801 aagatttaga agatacaatg tatgcacaag aagctgctgg cttatgtgca cctcaaatta
     1861 atcagtcatt gcaagtggca attattgata tggaaatgga aggattatta caacttgtta
     1921 atccgaaaat tattagtcaa tcaaatgaaa caataacaga cttagaaggt tcaattacat
     1981 tgccagatgt ttacggcgaa gtgacaagaa gtaaaatgat agttgtcgaa agttatgacg
     2041 tcaatgggaa caaagttgaa ctaactgcac atgaagatgt agcaagaatg attttgcata
     2101 ttatagatca aatgaacggt atccctttta cagaacgtgc ggaccgtatt ttaacagata
     2161 aagaagtgga ggcatatttt ataaatgact aaaataatat ttatgggtac accagacttt
     2221 tcaacaactg ttttagaaat gcttattgca gaacatgatg tcattgcagt cgtaacgcaa
     2281 ccagatcgac ctgttggacg taaacgtgtt atgacaccac caccagttaa aaaagttgca
     2341 atgaaatatg atttacctgt atatcagcct gaaaaattaa gtggatcaga agaattagaa
     2401 caattgcttc aattagatgt agatttaatt gtaactgctg cttttggaca attattacct
     2461 gaatcattgt tggcattacc aaaacttggc gcaattaatg tacatgcatc attgttacca
     2521 aagtatagag gtggtgcacc aattcatcag gcaattatcg atggtgaaca agaaaccggc
     2581 ataacaatta tgtatatggt taaaaaatta gatgcgggta atattatttc gcaacaagca
     2641 attaaaatag aagaaaatga taatgtcggt acgatgcatg ataaattaag tgtattaggg
     2701 gcagatttat taaaagaaac tttaccatct attatagagg gcacaaatga aagtgtacct
     2761 caagatgata cgcaagcaac atttgcttcc aatattcgac gcgaagatga gcgaattagc
     2821 tggaataaac caggaagaca agtgtttaat caaattcgtg gattatcacc atggccagtt
     2881 gcttatacaa ctatggatga cactaacttg aaaatatacg atgctgaact cgttgagact
     2941 aataagataa acgagcctgg aaccattata gaaacgacta aaaaagccat tattgttgct
     3001 acaaatgata atgaagctgt tgcaattaaa gatatgcaat tagctgggaa aaagagaatg
     3061 ttagctgcca attatttaag tggtgcgcaa aacacactag tagggaagaa acttatatga
     3121 tcgaaaacgt gagaagtctt gcttttgaca cgattcaaga tatattaaat gaaggtgcgt
     3181 atagtaactt gcgtatcaat gaagtgttgt cagaaaatga attaaatgca atggataagg
     3241 ctttatttac agaaattgtc tacggaaccg ttaaaagaaa atatacgtta gatttttatt
     3301 taaagccttt tgtgaaaaca aagattaagg catgggttag gcaattatta tggatgagta
     3361 tttatcaata tgtatattta gataaagttc caaatcatgc cattattaat gaagcagttg
     3421 aaatagcaaa agaacgcggt ggctatcata atggtaatgt cgtaaatggt attttacgta
     3481 caatgatgcg tagtgactta cctgatttta atgaaattgc agatcctaaa aaaagaatgg
     3541 caatcgagta tagtatgccg aagtggatta tagatcattg ggcaacacat tatggtctcg
     3601 aagaaactga aacaatttta cagtcatttt tagaaacgac atcaacaact gtgcgtgcca
     3661 acctgacgcg agcatcatta gatgatatta ttgaaaagtt gcaagacgaa ggttatgacg
     3721 ttgaaaaaga tcatgactta ccttattgtc tccatatagg aggacaacca attattcatt
     3781 ctcgttcatt taaagatgga ttcgtttcaa ttcaagataa aagctcaatg tttgttgcac
     3841 acattatgaa tgtagaccga catgatcacg tattagatgc atgtagtgca cctggcggta
     3901 aagcttgtca cattgctgaa gttttaatgc cagaagggca agttgacgct tcagatatac
     3961 atgatcacaa aatagactta attaatttta atataaaaaa
//
LOCUS       DAOBBH010000198         3989 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.198, whole genome shotgun sequence.
ACCESSION   DAOBBH010000198 DAOBBH010000000
VERSION     DAOBBH010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.198"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            14..937
                     /gene="graX"
                     /locus_tag="P6L01_002315"
                     /gene_synonym="apsX"
     CDS             14..937
                     /gene="graX"
                     /locus_tag="P6L01_002315"
                     /gene_synonym="apsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="auxiliary protein GraX/ApsX"
                     /protein_id="HDP3031361.1"
                     /translation="MKPKVLLAGGTGYIGKYLSEVIENDAELFTISKYPDNKKTDDVE
                     MTWIQCDIFHYEQVVAAMNQIDIAVFFIDPTKNSAKITQSSARDLTLIAADNFGRAAA
                     INQVKKVIYIPGSRYDNETIERLGAYGTPVETTNLVFKRSLVNVELQVSKYDDVRSTM
                     KVVLPKGWTLKNVVNHFIALMGYTKGTFVKTEKSHDQFKIYIKNKVRPLAVFKIEETA
                     DGIITLILLSGSLVKKYTVNQGKLEFRLIKESAVVYIHLYDYIPRLFWPIYYFIQAPM
                     QKMMIHGFEVDCRIKDFQSRLKSGENMKYTK"
     gene            953..1627
                     /gene="graR"
                     /locus_tag="P6L01_002316"
                     /gene_synonym="apsR"
     CDS             953..1627
                     /gene="graR"
                     /locus_tag="P6L01_002316"
                     /gene_synonym="apsR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor
                     GraR/ApsR"
                     /protein_id="HDP3031362.1"
                     /translation="MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFN
                     PEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPF
                     YTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGDDTIFLSKTEMIIL
                     EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGK
                     GYMAHE"
     gene            1620..2660
                     /gene="graS"
                     /locus_tag="P6L01_002317"
                     /gene_synonym="apsS"
     CDS             1620..2660
                     /gene="graS"
                     /locus_tag="P6L01_002317"
                     /gene_synonym="apsS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase GraS/ApsS"
                     /protein_id="HDP3031363.1"
                     /translation="MNNLKWVAYFLKSRMNWIFWILFLNLLMLGISLIDYDFPIDSLF
                     YIVSLNLSLTMIFLILTYFKEVKLYKHFDKDKEIEEIKHKDLAETPFQRHTVDYLYRQ
                     ISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSR
                     INSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDD
                     YVYTDTKWCRMIIRQILSNALKYSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPR
                     IFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGKGTTVRLIFPLQNEI
                     VERMSEVTNLSF"
     gene            2804..3565
                     /gene="vraF"
                     /locus_tag="P6L01_002318"
     CDS             2804..3565
                     /gene="vraF"
                     /locus_tag="P6L01_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein VraF"
                     /protein_id="HDP3031364.1"
                     /translation="MAILEVKQLTKIYGTKKMAQEVLRDINMSIEEGEFIAIMGPSGS
                     GKTTLLNVLSSIDYISQGSITLKGKKLEKLSNKELSDIRKHDIGFIFQEYNLLHTLTV
                     KENIMLPLTVQKLDKEHMLNRYEKVAEALNILDISDKYPSELSGGQRQRTSAARAFIT
                     LPSIIFADEPTGALDSKSTQDLLKRLTRMNEAFKSTIIMVTHDPVAASYANRVVMLKD
                     GQIFTELYQGDDDKHTFFKEIIRVQSVLGGVNYDL"
     gene            3555..>3989
                     /locus_tag="P6L01_002319"
     CDS             3555..>3989
                     /locus_tag="P6L01_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3031365.1"
                     /translation="MTFNEIIFKNFRQNLSHYAIYLFSLITSVVLYFSFVALKYAHKL
                     NMTESYPIIKEGSQVGSYFLFFIIIAFLLYANVLFIKRRSYELALYQTLGLSKFNIIY
                     ILMLEQLLIFIITAILGIIIGIFGSKLLLMIVFTLLGIKEKVP"
BASE COUNT         1483 a          507 c          715 g         1284 t
ORIGIN      
        1 aggagtattt tagatgaaac ctaaagtttt attagcaggt ggaacaggat atattggtaa
       61 gtatttaagt gaagtgattg aaaatgatgc tgaacttttt actatatcaa aatatccaga
      121 caataaaaaa acagatgatg ttgaaatgac ttggattcag tgtgatatat ttcattacga
      181 acaggttgtt gcagcaatga atcaaataga tattgctgta ttctttatcg acccaacaaa
      241 gaattctgcc aaaataacac aatcatcagc aagagattta acattaatcg cagcagataa
      301 ttttggtcga gcagcggcta tcaatcaagt aaaaaaagta atctacatac ctgggagtcg
      361 ttatgataat gaaacaattg aacgcctagg tgcatatggc acacctgtag aaacaacaaa
      421 tttagttttt aaacgttctt tagttaatgt agaattacaa gtttcaaagt atgatgatgt
      481 tagatcaacg atgaaggtag ttttaccaaa gggatggaca ttaaagaacg ttgtaaacca
      541 ttttattgca ttgatgggtt acactaaagg aacttttgtg aaaacagaaa aatcacatga
      601 tcaatttaag atatatatta agaataaggt gcgaccgctc gcagtattta aaatagaaga
      661 aacagctgac ggaataataa ctttaatttt attgagtgga agtttagtga aaaaatacac
      721 agttaatcaa gggaagttag aatttagatt aatcaaagag tcggcagtcg tttatataca
      781 tctatacgat tatatccctc gattattttg gccgatttat tactttatac aagcaccaat
      841 gcaaaaaatg atgattcatg gctttgaagt tgactgccgg attaaagatt ttcaaagtcg
      901 attaaaatca ggagaaaata tgaaatatac taaatgatat tgggtgatat ggatgcaaat
      961 actactagta gaagatgaca atactttgtt tcaagaattg aaaaaagaat tagaacaatg
     1021 ggattttaat gttgctggta ttgaagattt cggcaaagta atggatacat ttgaaagttt
     1081 taatcctgaa attgttatat tggatgttca attacctaaa tatgatgggt tttattggtg
     1141 cagaaaaatg agagaagttt ccaacgtacc aatattattt ttatcatctc gtgataatcc
     1201 aatggatcaa gtgatgagta tggaacttgg cgcagatgat tatatgcaaa aacctttcta
     1261 taccaatgta ttaattgcta aattacaagc gatttatcgt cgtgtctatg agtttacagc
     1321 tgaagaaaaa cgtacattga cttggcaaga tgctgtcgtt gatctatcaa aagatagtat
     1381 acaaaaaggt gacgatacga tttttctatc caaaacagaa atgattatat tagaaattct
     1441 tattaccaaa aaaaatcaaa tcgtttcgag agatacaatt atcactgcat tatgggatga
     1501 tgaagcattt gttagtgata atacgttaac agtaaatgtg aatcgtttac gaaaaaaatt
     1561 atctgaaatt agtatggata gtgcaatcga aacaaaagta ggaaaaggat atatggctca
     1621 tgaataattt gaaatgggta gcttattttt tgaaatctcg catgaactgg atattttgga
     1681 tattgttttt aaacttgctt atgttaggca ttagtctaat cgattatgat tttccaatag
     1741 acagtttatt ttatattgtt tctttgaatt taagtttaac aatgattttt cttatattga
     1801 catattttaa agaagtaaaa ttatataagc attttgacaa agataaagaa atagaagaaa
     1861 ttaaacataa agatttagcg gaaacgccat ttcaacgtca tacagttgat tatttatatc
     1921 gtcaaatctc agcgcacaaa gaaaaggttg ttgagcaaca gttacaattg aacatgcatg
     1981 aacaaaccat tacagaattt gtgcacgaca taaaaacacc tgtgacagcc atgaaattat
     2041 taattgatca agaaaaaaat caagaaagaa aacaggcatt actatatgaa tggtctcgta
     2101 taaactcgat gctggataca cagctgtata ttactagatt agaatctcaa cgcaaagata
     2161 tgtattttga ttacgtgtca cttaaacgca tggtcattga tgaaatacaa ttaacaagac
     2221 atattagtca ggttaaaggt attggttttg atgttgactt taaagtggat gattatgttt
     2281 atacagatac aaaatggtgt cgtatgatta ttagacagat tttgtcaaac gcattgaaat
     2341 atagtgagaa ttttaatatt gaaattggga cagaattaaa tgatcaacat gtttcgttat
     2401 atattaaaga ctatggcaga ggtattagta aaaaagatat gccgcgaata tttgaacgag
     2461 gatttacgtc aacggctaac agaaatgaaa cgacgtcttc aggtatgggt ctatatttag
     2521 taaatagtgt aaaggatcaa ttaggtattc acctgcaagt cacgtcgact gttggtaagg
     2581 ggacaactgt cagattgatt ttcccattac aaaatgaaat tgttgaacgc atgtcggaag
     2641 tgacaaattt gtcattttaa acatgcgttt tgttacttag aattgataca tcaatgcagc
     2701 ttcaacgtta taataagata gatgttagtc atatgttaaa ttgaagatac aagtgccaaa
     2761 gcctaaagga aatgaagtta agataaatta taggagtgtt aaagtggcaa ttttagaagt
     2821 aaaacaatta acaaaaatat atggaactaa aaaaatggca caagaagtgt tgcgagatat
     2881 caatatgtct attgaagaag gcgagtttat tgctattatg ggtccctctg gatctgggaa
     2941 aacgacatta ttaaatgttt taagttcaat tgattatatt tcacaaggtt ctattacatt
     3001 aaaaggaaaa aaattagaaa agctttcaaa caaggaatta tctgatatac gcaagcatga
     3061 tattggtttt atttttcaag agtataattt actgcataca ttgactgtta aagaaaacat
     3121 aatgttacca ctaacggttc agaagttaga taaagaacat atgttaaatc gttatgaaaa
     3181 agtagcagaa gcattaaata tattggatat tagtgataaa tacccttctg aattgtctgg
     3241 tggacaaaga caacgaacat ctgctgcaag agcgtttatt acattacctt ctattatatt
     3301 tgctgacgaa ccaacaggtg cactggattc taaaagtact caagatttat taaaacgatt
     3361 aacaagaatg aatgaagcat ttaagtctac aattattatg gtaacgcatg atcctgttgc
     3421 agcaagttat gccaatcgag tagtgatgct aaaagatggt caaattttca ctgaattata
     3481 ccaaggggat gacgataaac ataccttttt caaagaaata atacgtgtac aaagtgtttt
     3541 aggtggcgtt aattatgacc tttaacgaga taatatttaa aaatttccgt caaaatttat
     3601 cacattatgc catctatctt ttttcattaa ttacgagtgt agtattgtat tttagctttg
     3661 tagcattaaa atacgcgcat aaactaaaca tgacagagtc atatccaatt attaaggaag
     3721 gctcacaagt cggaagctac tttctatttt tcatcataat tgcatttttg ttatatgcca
     3781 atgtgttatt tattaaacga cgaagttatg agcttgcatt atatcaaaca ttaggtttat
     3841 ctaaattcaa cattatttat atactaatgc tcgaacaatt actaatattt ataattacgg
     3901 caatattagg tattattatt ggtatttttg gttcaaaact gttattaatg attgtcttta
     3961 cattattagg aattaaagaa aaggttcca
//
LOCUS       DAOBBH010000199         3943 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.199, whole genome shotgun sequence.
ACCESSION   DAOBBH010000199 DAOBBH010000000
VERSION     DAOBBH010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.199"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(111..308)
                     /locus_tag="P6L01_002320"
     CDS             complement(111..308)
                     /locus_tag="P6L01_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031366.1"
                     /translation="MNCYDEIFNTIKKLIENKEISSYQINKDTGISYGNINAMRRGER
                     RIENLSLKNAKILYEYAKKVL"
     gene            complement(801..2141)
                     /gene="glnA"
                     /locus_tag="P6L01_002321"
     CDS             complement(801..2141)
                     /gene="glnA"
                     /locus_tag="P6L01_002321"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDP3031367.1"
                     /translation="MPKRTFTKEDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQL
                     EKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTD
                     GTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFD
                     LAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKL
                     VVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQF
                     TAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRI
                     EVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPS
                     TLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY"
     gene            complement(2160..2528)
                     /locus_tag="P6L01_002322"
     CDS             complement(2160..2528)
                     /locus_tag="P6L01_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDP3031368.1"
                     /translation="MISNDAIRRNMAVFSMSVVSKLTDLTPRQIRYYETHELIKPERT
                     EGQKRLFSLNDLERLLEIKSLLEKGFNIKGIKQIIYDSQEHLTTDEQEIRKKMIVDAT
                     QKPIGETLPINRGDLSRFIK"
     gene            complement(2771..>3943)
                     /locus_tag="P6L01_002323"
     CDS             complement(2771..>3943)
                     /locus_tag="P6L01_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDP3031369.1"
                     /translation="EETAYINQEKVLNAFHHVKATESDLQGSTGYGYDDFGRDHLEEI
                     YAQAFKAEDAIVRPQIISGTHAITIALQSLLKHGDELIYITGSPYDTLLEVIGVNGNG
                     IESLMEHGVSYKDIALKEGKIDIESVLDGVSERTKVIAIQRSKGYDQRPSIPLDEIEK
                     VITRLKNVHPNILIFVDNCYGEFVERREPIECGADLIAGSLIKNPGGGLAKIGGYIAG
                     RKDLIERCGYRLTAPGIGKEAGASLNALLEMYQGFFLAPHVVSQSLKGALFTSLFLEK
                     MNMNTTPKYYEKRTDLIQTVKFETKEQMISFCQSIQHASPINAHFSPEPSYMPGYEDD
                     VIMAAGTFIQGSSIELSADGPIRPPYEAYVQGGLTYEHVKIAVTRAVNQLKEQGLI"
BASE COUNT         1204 a          762 c          550 g         1427 t
ORIGIN      
        1 tcactaaaca tgatttcttt cacttttaga aaacttttaa tactataaaa acacccactc
       61 agtcactagt ttgggcagtt attgtatgcc tattgaactc aatgcttata ttacaatacc
      121 tttttcgcat attcatataa gatctttgca ttctttaagc ttaaattttc tattcttctt
      181 tccccacggc gcatagcatt aatattaccg taacttatcc cagtatcttt attaatttga
      241 taacttgata tctctttgtt ttctatcaat tttttgattg tattgaatat ttcatcatag
      301 caattcataa attagatgag gcgaaatttt taatttttta gaatatcaat agtagtataa
      361 ctaaaatgaa aataccgatc gataaacaaa aagatatttt ttgttttgtt tctcttttca
      421 tatagtatta cccccttaat aatgcgtagt aaggtccctc ttttcggggt cttaccttag
      481 aacgttctgc aaatgaattc gatgagaagt aatatgaata tggctatttt caagtaatac
      541 tcaacgtttt cgcgacgttc ttttatcgcc tcatctcatc acctccaaat atattaaaat
      601 tcatgtgaac taaaatataa aatggtcttc cccagcttta aaaaaataaa tacataaaac
      661 atttacttga ccaaaacttg accaaatcca ttttttaaca aaagtggtca agtgaaatcc
      721 catctttcca aatcaaaaaa gagctaaagc aaaacgctct agcccttgat attactgatt
      781 tcccatcatt gtttagcgta ttaatattgc ttcatgtact gatctctttc ccattcagat
      841 acttgagttc tgtagtaatc ccattcaatt gattttgaat taataaattg attatagata
      901 tgatttccta aagctttttt aataacttca ttttcacgca ttgcttttaa tgcagtataa
      961 agtgttgaag gtaagtcttg aatgcctact gcttcacgtt cttcacggtt catttcgtaa
     1021 atattttggt taactggttc tggaactttt aatttatttt taataccatc tagtccagct
     1081 tctaagattg cagctaacgc catgtatggg tttgcagctg gatctactga acgtacttcg
     1141 atacgagtag ataatcctct tgaagatggt acacggatta atggtgaacg gtttttacca
     1201 ctccatgcaa tataacatgg tgcttcataa ccaggtacta aacgtttata tgagtttact
     1261 aacgggttac atacagcagt aaatccgcgt gcatttttaa gcacacctgc tgtaaattga
     1321 tatgcagttt ccgttaagcc catttcagta tttggatcaa agaatgcatt ttctttacct
     1381 ttgaataatg aaacgttaaa gtgcataccg ctaccattca caccgaataa tggtttaggc
     1441 ataaatgttg cgtgtaaatt atgtttacgt gcgattgttt taacaaccaa tttaaatgtt
     1501 tggatattat cacatgctgt aacagcatct gcatatttaa agtcaatttc atgttgacca
     1561 ggggcaactt catggtgact agcttcaata tcgaagccca tatcctctaa ttctaaaaca
     1621 atatcacgac gacagttttc acctaaatct gtaggtgcta aatcgaaata tccaccatca
     1681 tcattaagtt ctaaagttgg ttcccctttt tcatccaact taaacaagaa gaattctggt
     1741 tcaggcccta ggttaaagtc tgtgaagcct aaatcttcca tttcttttaa tacacgtttt
     1801 aagtttgcac gaggatcccc ttcaaatggt gttccatctg ttttatatac atcacaaatt
     1861 aaacgtgcaa ctttaccttg tccagcagtc catgggaaga ttacccaagt atctaaatct
     1921 ggatgtaagt acatatctga ttcttcgata cgtacgaaac cttcgataga agaaccgtca
     1981 aacatcattt cgttatcaag tactttttct aattggctta caggcacttc aacattttta
     2041 attgttccta aaatatcagt gaattgtaat cttaaatatc ttacattttc ctcttctgca
     2101 aatttacgaa tgtcttcttt agtgaaagta cgttttggca ttttaaaatc ctccaaattt
     2161 tatttaataa atcgggataa atcaccacga tttattggca aagtttctcc aataggcttt
     2221 tgcgtggcat ctacaatcat cttttttctt atctcttgtt catctgttgt taaatgctct
     2281 tgtgagtcat aaatgatttg tttaatccct ttgatattaa atcctttttc taataatgat
     2341 ttaatttcta gtaatctttc caaatcattg agtgagaaca gacgtttttg accttctgtt
     2401 ctttcaggtt tgatgagttc atgtgtttca tagtaacgta tttgccttgg cgttaaatcc
     2461 gttaacttac ttactacact catagagaag acagccatat ttcgtctgat tgcatcattc
     2521 gatatcattg ttcctctcct ctacttttga acttgttaat aacttatcat aattttttta
     2581 ggatttcaaa aatatgtcag gttttctaac ataaagctta aaacattgat ataactgcga
     2641 tttcgacttc taaaacgaca taaaacaatc tacagctaaa atattttttc aatttttaaa
     2701 aataatctga taaatctccg caaaaaattt cacccaagac acattatcat ttagggtgaa
     2761 atttaatcta ttatataagt ccttgttctt tcaactgatt aacagctctt gtcacagcaa
     2821 ttttaacgtg ttcatatgtt aatcctcctt gaacatatgc ttcataagga ggacgaatag
     2881 gtccatctgc agataattca atcgatgaac cttgaataaa cgtaccagct gccataataa
     2941 catcatcttc gtaaccaggc atataactag gttctggact aaaatgtgca ttaattgggg
     3001 atgcgtgttg aatactttga caaaatgaaa tcatttgttc tttcgtttca aatttaactg
     3061 tttgaattaa atcagttcgt ttttcgtagt acttcggcgt tgtgttcata ttcatttttt
     3121 ctaaaaataa actagtaaac aatgcacctt taagactctg actgacaacg tgtggtgcta
     3181 aaaagaaacc ttgatacatt tcaagcaatg catttaatga tgcacccgct tctttaccaa
     3241 taccaggtgc tgtcaatcta taaccacatc gttcaattaa atcttttcta ccagcaatgt
     3301 atccaccaat cttagctaaa ccaccgccag ggtttttaat taatgatcct gctattaaat
     3361 cggcaccaca ttctataggt tcacgtcttt caacaaattc cccataacag ttatccacaa
     3421 atattaaaat attaggatgc acgtttttca acctagtaat taccttttca atttcatcta
     3481 gcggaattga aggtctttga tcatagcctt tcgaacgttg aatcgctatt actttggtgc
     3541 gctcagaaac cccatctaac acactttcaa tatcgatctt accttcttta agtgcaatat
     3601 ctttatacga tacgccgtgc tccattaaac tttcaatacc atttccgttt acgccaatga
     3661 cttcaagtaa agtgtcatat ggactacccg ttatataaat taattcatca ccatgtttta
     3721 ataaactttg taatgcaata gtaatcgcat gcgtacctga aataatttgc ggacgaacaa
     3781 ttgcatcttc tgctttaaat gcctgcgcat atatttcttc taaatgatca cgtccaaagt
     3841 catcataccc gtatcctgtt gatccttgta gatcactttc ggttgctttg acatgatgaa
     3901 atgcatttaa tactttttct tgattaatat atgctgtttc ttc
//
LOCUS       DAOBBH010000200         3914 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.200, whole genome shotgun sequence.
ACCESSION   DAOBBH010000200 DAOBBH010000000
VERSION     DAOBBH010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.200"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            145..2082
                     /gene="thrS"
                     /locus_tag="P6L01_002324"
     CDS             145..2082
                     /gene="thrS"
                     /locus_tag="P6L01_002324"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDP3031370.1"
                     /translation="MEQINIQFPDGNKKAFDKGTTTEDIAQSISPGLRKKAVAGKFNG
                     QLVDLTKPLETDGSIEIVTPGSEEALEVLRHSTAHLMAHAIKRLYGNVKFGVGPVIEG
                     GFYYDFDIDQNISSDDFEQIEKTMKQIVNENMKIERKVVSRDEAKELFSNDEYKLELI
                     DAIPEDENVTLYSQGDFTDLCRGVHVPSTAKIKEFKLLSTAGAYWRGDSNNKMLQRIY
                     GTAFFDKKELKAHLQMLEERKERDHRKIGKELELFTNSQLVGAGLPLWLPNGATIRRE
                     IERYIVDKEVSMGYDHVYTPVLANVDLYKTSGHWDHYQEDMFPPMQLDETESMVLRPM
                     NCPHHMMIYANKPHSYRELPIRIAELGTMHRYEASGAVSGLQRVRGMTLNDSHIFVRP
                     DQIKEEFKRVVNMIIDVYKDFGFEDYSFRLSYRDPEDKEKYFDDDDMWNKAENMLKEA
                     ADELGLSYEEAIGEAAFYGPKLDVQVKTAMGKEETLSTAQLDFLLPERFDLTYIGQDG
                     EHHRPVVIHRGVVSTMERFVAFLTEETKGAFPTWLAPKQVQIIPVNVDLHYDYARQLQ
                     DELKSQGVRVSIDDRNEKMGYKIREAQMQKIPYQIVVGDKEVENNQVNVRQYGSQDQE
                     TVEKDEFIWNLVDEIRLKKHR"
     gene            2508..>3914
                     /locus_tag="P6L01_002325"
     CDS             2508..>3914
                     /locus_tag="P6L01_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDP3031371.1"
                     /translation="MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHD
                     AGALGALIGYAIIGIMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYW
                     FNWVVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWL
                     ALIKVITVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLI
                     AGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSAL
                     MGGSDNVATSPFTLVFKNAGFAFAASFMNAVILTSVLSAGNSGMYASTRMLYSMSKDK
                     LAFEAFGKTNKNGVPYMSLLVTAIIVVIIFVLQSLTKGAYEYIVAASGLTGFIAWVGI
                     AVSHYRFRRAFDKQNYDKSKLKYTAKLFPFGPLFAGFLCIVVIIGQDVDFIKTGDFNF
                     SRFLITYMGIPVFLAFFIY"
BASE COUNT         1291 a          530 c          808 g         1285 t
ORIGIN      
        1 ttgttttttt cagacataaa tgtttgttgg tgtcatatat tccctgttta ttgttaatag
       61 gtttaatgtt aaaacgatga ttgttgttca attttttaac gaggtcagat aaaagtattt
      121 ataaatcaaa taggagggtt taacatggaa caaattaata ttcaatttcc agatggtaat
      181 aaaaaggcgt ttgataaagg tactactact gaagatatag cacaatcaat tagtcctgga
      241 ttacgtaaaa aagctgttgc cggcaaattt aacgggcaac ttgtagattt aactaaaccg
      301 cttgaaactg atggatcaat tgaaattgtg acaccaggta gtgaagaagc gttagaggta
      361 ttacgtcatt ctactgcaca tttaatggca cacgcgatta aaaggttata tggtaatgtt
      421 aaatttggtg taggtcctgt aatagaaggt ggattctact atgacttcga cattgaccaa
      481 aacatctcat ctgatgactt tgaacaaatt gaaaaaacaa tgaaacaaat cgttaacgaa
      541 aatatgaaaa tcgaacgaaa agtggtttca cgagatgaag cgaaagagtt attcagcaat
      601 gatgaataca aattagaatt aatcgacgcg attcctgaag atgaaaatgt aacattatat
      661 agtcaaggtg attttactga tttatgtcgt ggagttcacg ttccatcaac agctaaaatt
      721 aaagagttta aactattatc tacagcaggt gcatactggc gtggagatag taacaacaaa
      781 atgttacaac gtatatacgg tactgctttc tttgataaaa aagaattaaa agcacattta
      841 caaatgttag aagagcgtaa agaacgtgat catcgtaaaa ttggtaaaga gttagaatta
      901 ttcacaaata gccaattagt tggtgctggt ttgccattat ggttacctaa cggtgcaaca
      961 attagacgtg aaattgaacg ttacattgtt gataaagagg tgagcatggg ttatgaccac
     1021 gtttatacac cagtacttgc taatgttgat ttatacaaaa catctggtca ctgggatcac
     1081 tatcaagaag atatgttccc accaatgcag ttagatgaaa ctgaatctat ggtattacgt
     1141 ccaatgaact gtccacatca tatgatgatt tatgcgaata aaccacattc atatcgtgaa
     1201 ttacctatcc gtatcgctga gctaggaacg atgcatagat atgaagcaag tggtgctgta
     1261 tcaggattac aacgtgttcg tggtatgact ttaaatgatt cacatatctt tgttcgacct
     1321 gatcaaatta aagaagaatt caaacgcgtt gtaaacatga ttattgatgt gtataaagac
     1381 tttggtttcg aggattatag ctttagatta agttatagag accctgaaga taaagaaaag
     1441 tactttgatg atgatgacat gtggaataaa gctgaaaata tgcttaaaga ggcagcggat
     1501 gagcttggct tatcgtacga agaagcgatt ggtgaagcgg cattctatgg tccgaaacta
     1561 gatgttcaag ttaaaacagc gatgggtaaa gaagagacat tatcaacagc acaacttgat
     1621 ttcttattac cagaacgttt tgatttaact tatattggtc aagatggtga acatcatcgt
     1681 ccagttgtta ttcatcgtgg tgttgtatca acaatggaac gatttgtagc atttttaact
     1741 gaagaaacta aaggtgcatt cccaacttgg ttagcgccaa aacaagttca aatcattcca
     1801 gttaacgttg atttacatta tgattatgcg cgccaattgc aagatgaatt gaaatctcaa
     1861 ggtgttcgtg taagtattga tgaccgtaat gaaaaaatgg gttataaaat cagagaagct
     1921 caaatgcaaa aaatacctta tcaaatcgta gttggggata aggaagttga aaataatcaa
     1981 gtgaatgtgc gtcaatatgg atcgcaagac caagaaacag ttgaaaaaga tgaatttatc
     2041 tggaatctag ttgatgaaat tcgtttgaaa aaacatagat agacagttgt cgcaataaaa
     2101 tgctttaaaa cttttattgc gtatcaagtt ttacagggtt gattatgcgt gatgaatcct
     2161 gtatattaca agttagttaa aatattaaat tgagttagag gttgcatgtt taattagtaa
     2221 cttgtcagaa gtatttatgg tacataagtt gaacaagtga aaggtaaaga tgccgaaata
     2281 gatataaacc ataaattata tctattggga cagttttcga ataggaactg tactgtcaca
     2341 gaatgtgatg tgctacctta tatagataat tgccaaagtg gttgcatatc ttaaaggtat
     2401 gtagccactt ttttactttt aatatcacta tgttctgtaa aaaagggtat gaaagtgaat
     2461 aaaggttatt tatttcttgg cctctaaaac atggaaaggg agcttatatg tcaaaagttc
     2521 aaaatgaaag taacaatgtt gtcaaaaggg gacttaaaga tcgtcatatt tctatgattg
     2581 cgattggggg ttgtattggt acaggtttat ttgtaacttc tggtggagca attcatgatg
     2641 caggtgcttt gggtgcatta ataggatacg caattatcgg aataatggta tttttcttaa
     2701 tgacgtcact tggtgaaatg gctacgtatt taccagtatc aggttcattt agtacatatg
     2761 ctacaagatt tgttgatcca tctttagggt ttgcgcttgg ttggaactat tggtttaact
     2821 gggtagtaac tgtagcagca gatattacga ttgcagcaca agtcattcaa tattggacac
     2881 cattgcaagg catacccgct tgggcatgga gtgcgttgtt tttagttata atttttagtc
     2941 tgaattcgtt atcagttcgc gtctatggtg aaagtgaata ctggttggca ttgataaaag
     3001 tgattacagt tattgttttc attgcaattg gtttattaac gattgtagga atcatgggtg
     3061 gtcatgttgt aggatttgaa atatttaata aaggtgaagg tcctattctt ggtggcaact
     3121 taggaggaag tttgttatca attctaggtg tattcttaat cgctggtttc tcattccaag
     3181 gtactgagtt aattggtatt acggctggtg aatcagaaaa tcctgaacgt gctgtgccga
     3241 aagcaattaa acaagtattc tggagaattt tattatttta cattttagcc atttttgtta
     3301 tcggtatgtt aattccttat gatagtagtg cattaatggg tggtagtgat aatgtagcaa
     3361 cgtctccatt cacattagtg tttaaaaatg ctggatttgc gtttgcagca tcatttatga
     3421 atgcagtcat tttaacgtct gtgttatcag caggtaactc aggtatgtat gcttcaaccc
     3481 gaatgctata ttcaatgagt aaagataaat tagcgtttga agcatttggt aaaacaaata
     3541 aaaacggtgt gccatatatg tcgttactcg ttacagcaat cattgtagtg attattttcg
     3601 tattacaaag ccttacaaaa ggtgcatacg aatatatcgt tgcagcaagt ggtttaactg
     3661 gttttattgc ttgggtaggt atagcagtaa gtcattatcg atttagaaga gcatttgata
     3721 aacaaaatta tgacaaatct aaattaaaat atactgctaa attattccca ttcggaccat
     3781 tatttgctgg ctttttatgt attgtagtaa ttattggtca agatgttgac tttattaaaa
     3841 caggagattt taattttagt cgcttcttaa ttacgtatat gggaattcca gtatttttag
     3901 cattctttat ttat
//
LOCUS       DAOBBH010000201         3911 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.201, whole genome shotgun sequence.
ACCESSION   DAOBBH010000201 DAOBBH010000000
VERSION     DAOBBH010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.201"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            77..1054
                     /locus_tag="P6L01_002326"
     CDS             77..1054
                     /locus_tag="P6L01_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3031372.1"
                     /translation="MIKFKNVTKRYGKHVAVDNISFNINEGEFFVLIGPSGCGKTTTL
                     KMINRLIHLSEGYIYFKDKPISDYPVYEMRWDIGYVLQQIALFPHMTIKENIAQVPQM
                     KKWKEKDIDKRVDELLDMVGLEPEKYKNRKPDELSGGQRQRVGVIRALAADPPVILMD
                     EPFSALDPISREKLQDDLIELQTKIKKTIIFVTHDIQEAMKLGDKICLLNEGHIEQID
                     TPEGFKNNPQSEFVKQFMGSHLEDDAPCVEENAIIRDLDIMKPIDEVTSMSAYPIVYD
                     NQPIEVLYQHLSESERVIVMQEDSVGQYVIDRKDIFKYLSQKKEVAQHD"
     gene            1047..2561
                     /locus_tag="P6L01_002327"
     CDS             1047..2561
                     /locus_tag="P6L01_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDP3031373.1"
                     /translation="MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILL
                     TKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYT
                     GIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIG
                     AGGLGDLILLGIDRNNASLILLGAIPAALLAIIFDLILRFMAKLSYKKLLMTLGVIVM
                     IIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAF
                     LFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYEQAKQSLEKKYQMTMLKPMK
                     YNNTYALAVKRDFAKQHNIRTIGDLNKVKDQLKPGFTLEFNDRPDGYKAVQKAYNLNL
                     DNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKE
                     SFLKKHPEIKKPLNKLENKISDEDMQMMNYKVTVKNEDPYTVAKDYLKAKGLIK"
     gene            2801..3859
                     /gene="hisC"
                     /locus_tag="P6L01_002328"
     CDS             2801..3859
                     /gene="hisC"
                     /locus_tag="P6L01_002328"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDP3031374.1"
                     /translation="MKEQLNQLSAYQPGLSPRALKEKYGIEGDLYKLASNENLYGPSP
                     KVKEAISAHLDELYYYPETGSPTLKAAISKHLNVDQSRILFGAGLDEVILMISRAVLT
                     PGDTIVTSEATFGQYYHNAIVESANVIQVPLKDGGFDLEGILKEVNEDTSLVWLCNPN
                     NPTGTYFNHESLDSFLSQVPPHVPVIIDEAYFEFVTAEDYPDTLALQQKYDNAFLLRT
                     FSKAYGLAGLRVGYVVASEHAIEKWNIIRPPFNVTRISEYAAVAALEDQQYLKEVTHK
                     NSVERERFYQLPQSEYFLPSQTNFIFVKTKRVNELYEALLNVGCITRPFPTGVRITIG
                     FKEQNDKMLEVLSNFKYE"
BASE COUNT         1447 a          522 c          742 g         1200 t
ORIGIN      
        1 gtaataaacg tggttaaata gggaatacat acatataatc tcggttttat gctatgagaa
       61 aaaaagaggt ggcaaagtga ttaagtttaa aaatgtaact aagcgttatg gcaaacatgt
      121 tgctgtcgat aacattagtt tcaatattaa tgagggtgaa ttttttgtgc taattggacc
      181 ttcaggttgt ggaaaaacta cgacattaaa aatgattaat cgactcattc acttaagtga
      241 aggttatatt tattttaaag ataaaccaat aagtgattat ccagtatacg aaatgcgttg
      301 ggatattgga tacgtattgc agcagattgc attattccca catatgacga tcaaagaaaa
      361 tattgcacaa gtgccacaaa tgaaaaagtg gaaagaaaaa gatatagata aaagagtaga
      421 tgaattactt gatatggttg gattagaacc tgaaaaatat aaaaacagaa aacctgatga
      481 attgtcaggg gggcaacgac aacgtgtagg agttatacgt gcgttagcag ctgatccacc
      541 agttatttta atggatgaac cgtttagtgc attagaccca atcagccgag aaaaacttca
      601 agatgattta attgaattac aaactaaaat taagaagaca atcatatttg ttacacatga
      661 tattcaagag gcgatgaaac ttggtgataa gatttgtctt ttgaatgaag ggcatattga
      721 acaaattgac acaccagaag gatttaaaaa taatcctcaa agtgaatttg ttaaacaatt
      781 tatgggtagt catttagaag atgatgcgcc atgtgttgaa gagaacgcaa ttattcgtga
      841 cttggatatt atgaaaccaa tcgatgaggt tacatctatg agcgcttatc caattgttta
      901 tgacaatcaa ccaattgaag tattgtatca acatttatca gagagcgagc gtgtcattgt
      961 catgcaagaa gatagcgtag gtcaatatgt tattgatagg aaagatatct tcaaatattt
     1021 gtcccagaaa aaggaggtag ctcaacatga ctaacttttt cgacatattg agtgaacgta
     1081 aggggcaact cttttcgaca atgatagaac atattcaaat atcatttatt gcattattga
     1141 ttgcaactgc tattgcggta ccattaggta ttttattaac gaagactaaa acgatatctg
     1201 aaatcgtaat gaatattgcg gcaattcttc aaaccatacc atcgttggca ttattaggtt
     1261 taatgattcc tttatttggt atcggtcgtg tgccagcaat tattgcactt gtagtgtatg
     1321 cgttgttacc aattttaagg aatacgtata ctggaattaa agaagttgat ccatcactca
     1381 ttgaagcggc taaaggtata ggtatgaaac catttagacg tttaactaaa gtcgaacttc
     1441 cgatagcaat gcctgttata atggctggtg taagaacggc tatggtatta attataggta
     1501 cagcgacact agcagcatta attggtgcag gcggactagg agatttaatt ttattaggta
     1561 tagaccgtaa caatgcgtca ttgatattat taggtgcaat tccagcagcc ttattggcaa
     1621 ttatatttga tttaatttta agatttatgg ctaaattatc ttataaaaag ttattgatga
     1681 cgttaggtgt tatagtgatg attattatac tggccatcgc tattcctatg tttgcacaaa
     1741 aaggtgataa aattacgtta gctggaaagc ttggctccga gccttcgatt attacaaata
     1801 tgtataaaat tttaatagaa gaagagacca aaaatactgt agaagtgaaa gatggtatgg
     1861 ggaaaacagc atttttattt aatgctttaa aatctgatga tatagatggg tatttagagt
     1921 ttactggaac agttttaggt gaattaacaa aagaaccatt gaagtcaaaa gaagagaaaa
     1981 aagtttatga acaagctaag caaagtcttg aaaagaaata tcaaatgact atgttaaaac
     2041 caatgaagta taacaatacg tatgctttag ctgtaaaacg tgattttgct aaacaacata
     2101 atatacgtac aattggtgat ttaaataagg ttaaagatca acttaaacca ggatttacat
     2161 tggaatttaa tgatcgtcca gatggttaca aagctgttca aaaggcttat aatttaaatt
     2221 tagataacat acgtacaatg gaacctaagt tgagatatca agcgatcaat aaaggtaata
     2281 ttaatttaat agatgcatat tcaactgacg ctgaattaaa acaatatgat atggttgtgt
     2341 taaaagatga taagcacgta tttccaccat atcaaggagc accattattt aaagaaagct
     2401 ttttaaagaa acatccagaa attaagaaac cgttaaacaa actagaaaac aaaatatctg
     2461 atgaagatat gcaaatgatg aactataaag taacagttaa aaatgaagac ccatatacag
     2521 ttgcgaaaga ttatttaaaa gcaaaagggt taatcaaata acgaccaacg ccacataaga
     2581 tgcgtaacac caaattatat cttatgtggc gttgttatat ttaaatctat aattatgttc
     2641 aatttaaaca tgcaataatg attaaaaaat atgacatgtt aaacacaatg taagctatta
     2701 tgatgtgaaa atagtagcat tgcattttag aaacatagag cgatataatg aatataagtt
     2761 ttttaaaatt tcagttaatt ctaaggaggt tgtttttatt atgaaagaac aacttaatca
     2821 actatcagca tatcagcctg gtttatctcc aagggcattg aaagaaaagt atggcattga
     2881 aggagattta tataaacttg catcaaatga aaatttgtat ggaccatcgc ctaaagttaa
     2941 agaagcgata tcagcacatt tagatgagtt atattattat cctgaaacag gatcaccgac
     3001 attaaaagcg gcgattagta aacatttaaa tgtagatcaa tcacgcattt tatttggtgc
     3061 gggattagat gaagttatat taatgatttc tagagcagta ttaacgccag gggatactat
     3121 tgttacaagt gaagcgacat tcggtcaata ttatcacaat gcgattgttg aatcagctaa
     3181 tgtgatacaa gtacctttaa aagatggtgg cttcgattta gaaggtattt taaaagaagt
     3241 taatgaagat acgtcattgg tatggttatg taatccaaat aatcctacag gtacatattt
     3301 taatcatgag agcttagatt cgtttttatc tcaagtacct ccacatgtac cagtaattat
     3361 agatgaagct tattttgaat ttgtgacagc agaggactac ccggatacac ttgctttgca
     3421 acaaaaatat gacaatgctt tcttattacg tacattttca aaggcgtatg gattagcggg
     3481 tttacgtgta ggatatgtgg tagcaagtga acatgcgatt gaaaaatgga acatcattag
     3541 accaccattt aatgtgacac gtatatctga atacgcagca gttgcagcac ttgaagatca
     3601 acaatattta aaagaggtaa cacataaaaa tagtgttgaa cgcgaaagat tttatcaatt
     3661 acctcaaagt gagtatttct tgccaagtca aacgaatttt atatttgtaa aaacaaagcg
     3721 ggtaaatgaa ctttatgaag cacttttaaa tgtagggtgt attacgcgac catttccaac
     3781 tggtgttaga attacaattg gttttaaaga acaaaatgat aaaatgttag aagttttatc
     3841 aaattttaaa tacgaatagt aagtggggag tgggacagaa atgatatttt ctcaaaattt
     3901 atttcgtcgt c
//
LOCUS       DAOBBH010000202         3900 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.202, whole genome shotgun sequence.
ACCESSION   DAOBBH010000202 DAOBBH010000000
VERSION     DAOBBH010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.202"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..311
                     /locus_tag="P6L01_002329"
     CDS             <1..311
                     /locus_tag="P6L01_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxine reductase"
                     /protein_id="HDP3031375.1"
                     /translation="KIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMS
                     EFIKFVLEDKGKAAEEAGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSEDKK
                     "
     gene            502..1428
                     /gene="pstC"
                     /locus_tag="P6L01_002330"
     CDS             502..1428
                     /gene="pstC"
                     /locus_tag="P6L01_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDP3031376.1"
                     /translation="MTSSTNVKALIEKNINKKGKHNDKIIPVILAAISAISILTTLGI
                     LITLLLETITFFTRIPITEFLFSTTWNPTGSDPKFGIWALIIGTLKITVIATIFAVPV
                     GLGAAIYLSEYASDRARRIIKPILEILAGIPTIVFGFFALTFVTPVLRSFISGLGEFN
                     AISPGLVVGIMIVPLITSLSEDAMASVPNKIREGAYGLGATKLEVATKVVLPAATSGI
                     VASIVLAISRAIGETMIVSLAAGSSPTASLSLTSSIQTMTGYIVEIATGDATFGSNIY
                     YSIYAVGFTLFIFTLIMNLLSQWISKRFREEY"
     gene            1430..2347
                     /gene="pstA"
                     /locus_tag="P6L01_002331"
     CDS             1430..2347
                     /gene="pstA"
                     /locus_tag="P6L01_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDP3031377.1"
                     /translation="METTDNNRQSLVDQQLVQKHLSSRTVKNKVFKLIFLACTLLGLV
                     VLIALLTQTLIKGVSHLNLQFFTNFSSSTPSMAGVKGALIGSLWLMLSIIPLSIILGI
                     GTAIYLEEYAKNNKFTQFVKISISNLAGVPSVVFGLLGYTLFVGGAGIEALKMGNSIL
                     AAALTMTLLILPIIIVSSQEAIRAVPNSVREASYGLGANKWQTIRRVVLPAALPGILT
                     GFILSLSRALGETAPLVLIGIPTILLATPRSILDQFSALPIQIFTWAKMPQEEFQNVA
                     SAGIIVLLVILILMNGVAIILRNKFSKKF"
     gene            2394..3245
                     /locus_tag="P6L01_002332"
     CDS             2394..3245
                     /locus_tag="P6L01_002332"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDP3031378.1"
                     /translation="MAQTLAQTKQISQSHTFDVSQSHHKTPDDTNSHSVIYSTQNLDL
                     WYGENHALQNINLDIYENQITAIIGPSGCGKSTYIKTLNRMVELVPSVKTAGKILYRD
                     QDIFDQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSL
                     RGAAIWDELKDRLHTNAYSLSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRV
                     EELVQELKEKYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTED
                     YISGRFG"
     gene            3252..3893
                     /gene="phoU"
                     /locus_tag="P6L01_002333"
     CDS             3252..3893
                     /gene="phoU"
                     /locus_tag="P6L01_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDP3031379.1"
                     /translation="MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGF
                     ARQTVKNDKHINQLNYDINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNASS
                     IANIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTVLIREIIERDEDIDDL
                     YSHIINATYLIDNDPFVAAQAHLAARHLERIGDHIINIAESVYFYLTGTHYEQ"
BASE COUNT         1382 a          605 c          685 g         1228 t
ORIGIN      
        1 ttaaaatcaa agatgaaaat ggtaaagcaa cagagcctac gaaaaaaaca attcaagata
       61 actcttatgc attaagtaga ccattattca tttatgtaaa tgaaaaagca ttgaaagata
      121 ataaagtaat gtcagaattt atcaaattcg tcttagaaga taaaggtaaa gcagctgaag
      181 aagctggata tgtagcagca ccagagaaaa catacaaatc acaattagat gatttaaaag
      241 catttattga taaaaatcaa aaatcagacg acaagaaatc tgatgataaa aagtctgaag
      301 acaaaaaata ataagacgca atttcaaatg tgtcttgaaa catgattttg atggtgaatc
      361 attatttaga gtacaaagct tgatttatcg agacgctgat tttgacattc agttagtcta
      421 caagcttatc aacttaaaat agtggttcat cattatttta caaatctaat tattttggga
      481 gtaatagaaa gaggtttgat tatgacttca tctactaatg ttaaagcttt aatcgaaaaa
      541 aatattaata aaaaagggaa gcataatgac aaaattatac cagttatttt agccgcaatt
      601 tcagcgattt ccattttaac aacactaggt atattaatca cattgctttt agaaaccatc
      661 acttttttca ccagaattcc aataactgaa tttctatttt ctactacttg gaatcctacc
      721 ggttcagacc ctaagtttgg tatctgggca ttgataatag ggactttaaa aatcacagtt
      781 attgcgacta tatttgcagt tccagtcggg cttggtgcag caatttattt aagtgaatat
      841 gcaagtgacc gtgcacgacg cataattaaa ccgatattag aaattttagc aggaatacca
      901 acaattgtgt ttggtttctt tgcattaacc tttgttacac cagtattaag atctttcata
      961 tcaggtcttg gagagtttaa tgctataagt cccggcttag ttgtcggtat tatgattgtc
     1021 cctctcatca caagtttgag tgaggatgca atggcatctg taccaaataa aattcgagaa
     1081 ggtgcctatg gacttggagc aactaaatta gaagtagcaa ctaaagtcgt acttcccgca
     1141 gcaacatcag gtattgtagc ttcaatcgtt ctcgcgattt caagagcaat tggagaaacg
     1201 atgattgtat cattagcggc aggtagttcg ccaacagctt cattaagttt aacaagttcg
     1261 attcaaacaa tgactggata tattgttgag atagcgacag gtgatgcaac atttggatca
     1321 aatatttatt acagtattta tgctgtaggg ttcacactat ttatctttac cttaatcatg
     1381 aatttacttt ctcagtggat ttctaagcgt tttagggagg agtattaata tggaaacgac
     1441 agataataat agacaatcac tcgtcgatca acaacttgtc caaaaacatt tatcatccag
     1501 aacggttaaa aataaagtgt tcaaactcat atttttagca tgtacattat taggacttgt
     1561 cgtacttatt gcgttgttaa ctcaaacatt gattaaaggg gtaagtcatt taaatttaca
     1621 gtttttcact aatttttctt cttcaacacc atctatggct ggcgttaaag gcgcgttaat
     1681 cggttcactt tggttaatgt taagtatcat tccattatca atcatcctag gaataggtac
     1741 agctatatac ttagaagaat atgcgaaaaa caacaaattt actcagtttg ttaaaatcag
     1801 tatttccaat ttagctggtg taccatcagt tgtatttggg ttattaggtt acactttgtt
     1861 cgttggtggt gcggggattg aagccttgaa aatgggtaac agtatattgg cagcagcgct
     1921 aacaatgacc ttactgatat taccaattat tattgtttca agtcaggaag caattagagc
     1981 tgtacctaac tcagtacgcg aagcttctta tggtttaggt gctaataaat ggcaaacgat
     2041 aagacgtgtt gtcttaccag cagcgttacc tggtatttta actggattca ttttgtctct
     2101 ttcaagagca ctgggagaaa cagcgccact tgtgctaatc ggtataccga ctatattatt
     2161 ggcaacacct agaagtatat tggatcaatt ttcagcatta ccaatccaaa tatttacttg
     2221 ggcgaaaatg cctcaagaag aattccagaa tgttgcatcg gcaggcatta tcgttttact
     2281 agttatctta atcttaatga atggcgttgc gattatttta cgtaacaaat ttagtaaaaa
     2341 attctaattt aaacaatcaa tctcatttat ctattaaaaa gggagtttta aatatggcgc
     2401 aaacacttgc acaaactaaa caaatatctc aaagtcatac gtttgatgtc tcacaaagtc
     2461 atcataaaac accagatgat acaaactcac attctgttat atattcaaca caaaatttag
     2521 acttatggta tggcgaaaat catgcattac aaaatattaa tttagatatt tatgaaaacc
     2581 aaattactgc cattataggt ccatctggtt gtggcaaatc aacatatatt aaaactttga
     2641 atcgaatggt tgagttagta ccttctgtga aaacagctgg taaaatatta tatcgagatc
     2701 aagacatttt tgatcaaaaa tattctaaag aacaattacg tacaaatgtg ggcatggtct
     2761 ttcaacaacc taatccattt ccaaaatcaa tatacgataa tattacttac ggtccaaaga
     2821 ttcacggtat taaaaataaa aaagttcttg atgaaatcgt tgagaaatca ttacgtggcg
     2881 ctgcaatttg ggatgaatta aaggataggt tgcacacaaa tgcatatagt ttatccggtg
     2941 ggcaacaaca acgtgtttgt atcgcgcgtt gtttagcaat tgaacctgaa gtcattttaa
     3001 tggatgaacc gacatcagca ttagatccaa tctcaacatt aagagtagaa gagttggttc
     3061 aagaactaaa agaaaagtat acaattatta tggttacaca taatatgcaa caagcagctc
     3121 gtgtatcaga taaaactgca tttttcttaa atggttatgt caatgaatat gatgatactg
     3181 ataaaatttt ctctaaccca tcaaacaaga aaacagaaga ttatatttca ggaaggtttg
     3241 gttgatatat aatggcaata attagacagc gatatcagga gcaacttgat gatttaataa
     3301 aggaattacg tcggttaggt gcaaatgtct atgtgagtat tgaaaatggt ataaaatcat
     3361 taagtattga cgatagaggc tttgcacgac aaacagttaa aaacgataaa catatcaatc
     3421 aattaaatta tgatattaat gagcgagtta tcatgttaat tacaaagcaa cagcccattg
     3481 cgagtgattt gcgtatgatg atttcttcat taaaaatcgc ctccgattta gaaagaatag
     3541 gagataatgc atcgagtatt gccaatattc gattgcgtac aaagattaca gatgattatg
     3601 tgttaacccg tttaaagaca atgggtaaat tagctatgtt aatgttaaag gacttagatc
     3661 aagcatttaa aaagaaagat accgtattaa taagagaaat aattgagcgt gatgaagata
     3721 tcgatgactt atatagtcat attattaacg caacgtatct tattgataac gatccatttg
     3781 tcgctgcaca agctcattta gcagcaagac atttagaacg tattggtgat catattatta
     3841 acatcgctga aagtgtttat ttttatttaa caggtacaca ttacgaacaa taacttaaag
//
LOCUS       DAOBBH010000203         3816 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.203, whole genome shotgun sequence.
ACCESSION   DAOBBH010000203 DAOBBH010000000
VERSION     DAOBBH010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.203"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(357..1364)
                     /locus_tag="P6L01_002334"
     CDS             complement(357..1364)
                     /locus_tag="P6L01_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HDP3031380.1"
                     /translation="MSYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKT
                     AILMAAVMNFIGALTFTGVAGTITKDIVDPFKLENGLVVVLAAILAAIIWNLATWFYG
                     IPSSSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFK
                     VIFKNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVE
                     PQLWVKFACATAMGLGTAIGGWKIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLH
                     FPLSTTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPISALLAGLLFYILNLF
                     F"
     gene            complement(1380..1997)
                     /locus_tag="P6L01_002335"
     CDS             complement(1380..1997)
                     /locus_tag="P6L01_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HDP3031381.1"
                     /translation="MFSKKKDKFMVQLEEMVFNLDRAAIEFGKMDFNTHLDLKAYSDN
                     IKTYESHGDELVHQVITDLNQTFITPIEREDILSLCDAIDDVLDAIEETAAMFEMYSI
                     EYTDEYMAEFVDNIQKAVAEMKLAVGLLVDKKLSHMRIHSINIKEFETNCDGILRQSI
                     KHIFNSETDPITLIKIKDIYESMEEIADKCQIVANNFETIIMKNS"
     gene            complement(2722..>3816)
                     /gene="vraG"
                     /locus_tag="P6L01_002336"
     CDS             complement(2722..>3816)
                     /gene="vraG"
                     /locus_tag="P6L01_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease VraG"
                     /protein_id="HDP3031382.1"
                     /translation="ISVNDVMFSSSIMYRIKKNAFSLTVMAIISAITVSVLCFAAISR
                     ASLSSEIKYTAPHDVTIKDQQKANQLASELNNQKIPHFYNYKEVIHTKLYKDNLFDVK
                     AKEPYNVTITSDKYIPNTDLKRGQADLFVAEGSIKDLVKHKKHGKAIIGTKKHHVNIK
                     LRKDINKIYFMTDVDLGGPTFVLNDKDYQEIRKYTKAKHIVSQFGFDLKHKKDALALE
                     KVKNKVDKSIKTRSEAISSISSLTGILLFVTSFLGITFLIAVCCIIYIKQIDETEDEL
                     ENYSILRKLGFTQKDMARGLKFKIMFNFGLPLVIALSHAYFTSLAYMKLMGTTNQIPV
                     FIVMGLYICMYAVFAVTAYNHSKRTIRHSI"
BASE COUNT         1301 a          651 c          483 g         1381 t
ORIGIN      
        1 ataatgattt aatttgttat accagtattt tacgcttttt cttctacata tacaaattta
       61 tattaaataa agcccaatac aatttaggtt aattaaacaa gttgataact atttaattat
      121 tccttcattg aagaatataa actattaaat cattatttta cgcttactta tattttaatg
      181 acctaactgt ttatgttcca tggaataaat ttataactta gccccctaat taaaatgctt
      241 tgtcttggtc attctacgta aattctataa aatatgttat ctacttacat aaaaaaatga
      301 acttcaatac caccatatgt ttgtgatact gaagttcagt ttagttttat tttcaattag
      361 aaaaataagt taagtatata gaatagtaaa cctgctaaca atgctgaaat aggtaatgta
      421 atcacccatg taatgatcat tcgttgcgca gtgctccatt ttacaccttt agctcggtta
      481 gaagcaccaa cacctaagat tgatgatgac acaacgtgag ttgttgataa tgggaaatgt
      541 agcgatgatg caacaaaaat tgttaatgca gatgataaat cggccgcagc accatttgct
      601 ggacgtattt tcataatatt accacctaca gttttgataa ttttccagcc accaattgca
      661 gtaccaagcc ccattgctgt cgcacaggca aattttaccc ataactgtgg ttcaacactg
      721 ccatcattct gtacattagc gacaatcaat gccaacgtaa taatacccat tgatttttgc
      781 gcatcattcg taccgtgaga gaatgattgt aacgctgccg tgaaaatttg gaaaaatcta
      841 aagttacgat tcgctcttgt taaatttgca tttttaaaga taactttaaa aattgaatac
      901 atcaagaaac caacacaaaa tgcgattatc ggtgaaacga ttaatacaat aataattttt
      961 gtgaaacctt ggtaatgtaa cactccaaat gagccttcag atgcgattgc tgcacccgca
     1021 attgaaccta taagtgcatg tgaagacgaa cttggaattc cgtaaaacca agtagctaaa
     1081 ttccaaataa tagccgcaag tattgcagct aacacaacaa ctaatccatt ttccaattta
     1141 aatggatcga caatgtcttt agtaatggtg cctgcaacgc ccgtaaatgt taaagcacct
     1201 ataaagttca tcactgctgc cattaaaatt gccgttttag gcgttaacgc tctagtagat
     1261 acagcagtag ctactgcatt ggctgtatca tggaatccat tgataaagtc aaatatcagc
     1321 gagaaaataa ctacagctat agtgacgatg attatatatg acataaatat atactcccct
     1381 tagctatttt tcataataat agtttcaaaa ttatttgcta cgatttgaca tttatcagca
     1441 atttcttcca tgctttcata aatatctttt attttaatta atgtgattgg atctgtttcg
     1501 ctattgaaaa tatgtttaat tgactgtctt aaaataccat cacagtttgt ttcaaattct
     1561 ttaatattaa ttgaatgaat acgcatatgt gataattttt tatcgactaa taagccgaca
     1621 gcaagtttca tttctgcaac tgctttttga atgttatcaa caaactcagc catatattca
     1681 tctgtgtatt cgattgaata catttcaaac atagctgccg tttcttcaat tgcatctaaa
     1741 acatcatcaa ttgcatcaca taatgataaa atatcttcac gttcaattgg tgtgataaat
     1801 gtttgattta aatcagtaat tacttgatgt actaattcgt caccatgtga ctcataagtt
     1861 ttaatgttgt ctgagtatgc ttttaaatct aaatgtgtat tgaaatccat tttaccgaat
     1921 tcaatagcag cacgatccag attgaaaacc atctcttcta attgaaccat aaacttatct
     1981 tttttcttac taaacattta aaatcctcca tttaagcgat tgtcaccaat cacattcagt
     2041 tataatttgt ttcaaattaa gacaagtgaa tttacaaact aatgatacaa atttgttatt
     2101 atcaatcgtc agtataattt tagtgtactg atattaattt caaaaatgcc tcacagtaaa
     2161 caatttactg tatttgccct tatatgaaaa tagcacaaaa tttacaatcc cacaattcaa
     2221 tttacaaatt tttaacaatt ccgaaactta acttttacat aaaatttaaa tagtagtagg
     2281 catttgtttt gaaaaagtta ttttataggc aattttttat ctatacaact ctaaaaattg
     2341 taaatggata tagttgggaa atttgcaacg atacaatatt tattacataa cttttacata
     2401 ttaaaataca cttatcttgt ttaaaacgat cattgactca tatatatctc aatgaatata
     2461 cttttatttt ctcacattta atacaattta caaagttgaa tcctgttaat cttttcaatt
     2521 aaaagcatca ccaattaaat atatttcatt caaaacactt tcataatcac tcataccttt
     2581 acttcattat taatatggta ttttaaccca gtattcaact atacatgcta aaaatataac
     2641 ttctcttcat ttataataaa aagcttccag tatagttcgt gaattcgaat ccactactct
     2701 actgaaagcc atctgtatat tttatatgga atgtctaatt gttcgcttgg aatgattata
     2761 agccgtcact gcaaaaacag catacataca aatgtataat cccattacta tgaaaaccgg
     2821 tatttgattc gttgtaccca ttaatttcat atatgctaat gatgtaaaat atgcatgtga
     2881 tagtgcaata actaaaggta acccaaaatt aaacataatt ttaaacttta gtccccttgc
     2941 catatctttt tgtgtaaatc caagctttct caatatacta taattctcta actcatcttc
     3001 ggtttcatct atttgcttaa tgtatataat gcaacataca gcaatcaaga atgtaatacc
     3061 taaaaatgat gttacaaata ataatattcc ggttaaactt gatattgagc ttatcgcttc
     3121 acttcttgtt ttaatagatt tatcaacttt atttttcact ttttctaatg ctaaagcatc
     3181 ttttttatgt ttcaaatcga atccaaattg agagacgata tgctttgctt ttgtatactt
     3241 tcttatttct tgatagtctt tgtcatttaa gacaaacgtt ggtccaccta aatcaacatc
     3301 tgtcataaaa tagattttat taatatcttt ccgtaactta atattaacat gatgtttttt
     3361 cgttcctata attgccttac catgcttctt atgtttcact aaatctttga tagaaccttc
     3421 cgctacaaac aaatcagctt gtccacgttt caaatcagta ttagggatat atttatcact
     3481 agtaattgtt acattgtatg gttctttcgc ttttacatca aataaattat ctttatacaa
     3541 tttcgtatga attacttctt tataattata aaaatgagga attttttgat tgtttaattc
     3601 acttgctaat tgattagctt tttgttggtc tttaattgta acgtcgtgtg gtgcagtata
     3661 ttttatttca cttgataagg acgctctact tatagcagca aagcaaagaa ctgaaacagt
     3721 aatcgctgaa atgattgcca tgaccgtaag tgaaaaagca tttttcttaa tacgatacat
     3781 aatagatgat gagaacatga catcatttac acttat
//
LOCUS       DAOBBH010000204         3788 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.204, whole genome shotgun sequence.
ACCESSION   DAOBBH010000204 DAOBBH010000000
VERSION     DAOBBH010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.204"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..876)
                     /locus_tag="P6L01_002337"
     CDS             complement(<1..876)
                     /locus_tag="P6L01_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEAT domain-containing protein"
                     /protein_id="HDP3031383.1"
                     /translation="MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQAAAEE
                     TGGTNTEAQPKTEAVASPTTTSEKAPETKPVANAVSVSNKEVEAPTSETKEAKEVKEV
                     KAPKETKAVKPAAKATNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKENG
                     EQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVK
                     DYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAE
                     KLLAPYKKAKTLERQV"
     gene            complement(1079..2131)
                     /gene="isdA"
                     /locus_tag="P6L01_002338"
     CDS             complement(1079..2131)
                     /gene="isdA"
                     /locus_tag="P6L01_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored heme-scavenging protein IsdA"
                     /protein_id="HDP3031384.1"
                     /translation="MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQV
                     NAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYY
                     FQTVLNNASFWKEYKFYNANNQELATTVVNDNKKADTRTINVAVEPGYKSLTTKVHIV
                     VPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEK
                     VKPTVTTTSKVEDNHSTKVVSTDTTKDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVAT
                     AKSESNNQAVSDNKSQQTNKVTKHNETPKQASKAKELPKTGLTSVDNFISTVAFATLA
                     LLGSLSLLLFKRKESK"
     gene            2340..3023
                     /gene="isdC"
                     /locus_tag="P6L01_002339"
     CDS             2340..3023
                     /gene="isdC"
                     /locus_tag="P6L01_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdC"
                     /protein_id="HDP3031385.1"
                     /translation="MKNILKVFNTTILALIIIIATFSNSANAADSGTLNYEVYKYNTN
                     DTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKENIISKNTAKDERTS
                     EFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPTDVAGANAPGKDDKN
                     SASGSDKGSDGTTTGQSESNSSNKDKVENPQTNAGTPAYIYAIPVASLALLIAITLFV
                     RKKSKGNVE"
     gene            3023..>3788
                     /gene="isdD"
                     /locus_tag="P6L01_002340"
     CDS             3023..>3788
                     /gene="isdD"
                     /locus_tag="P6L01_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated surface determinant protein IsdD"
                     /protein_id="HDP3031386.1"
                     /translation="MRNVKQIATKSIIAIISLCILTYTTMIGSVLADEIKYPSAKFNQ
                     PEAKDKTELTTSIFDEKIKENKALELLIFNQENKNVTEEQQLVDEKAQLISDMTGKIY
                     LQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDDTIVRILIEQHMDKINMHVKTLAEK
                     KNLDNKEMVYSIHFKEKKVQHDDAKEVPSKHQNQENNQDQLKKDIDDKKDSQKSDTKE
                     RRTSLFTEKGLNDIPVQKDKVQQDSNKKIENERPKAS"
BASE COUNT         1255 a          513 c          657 g         1363 t
ORIGIN      
        1 aacttgtctt tctagtgttt tcgctttttt atatggcgct aataattttt cagctttata
       61 atcttcttca gttttgaatt tatctgcact gttataaatt ggttgtgcga attccattaa
      121 tgtgtaatcg tatttttctt ctttgttatt gaagtgagtt gaacttacaa ttttaacggc
      181 ttttgttcca tttgaaacag agaagcgaat gtaagcgtaa tctttaacag tatcgtatga
      241 tactaattta attggcaact ttttgtcacc ttcataaact tcaaattttc tccaaaattg
      301 acctgattgt aatcctaatt caatttctgg ttttgaatca gtgaaaataa ctctagcagg
      361 tttaacagag ctggcataat gataaaattg ttgctcacca ttttcttttt tcatttcaaa
      421 atcaattgga cgagagtttg gtgcgctatg atctttatct tttattgcag ggtttttaat
      481 cgcttctcta agttcctgat tcaaaatagg atatgtattg ttagtggctt ttgctgctgg
      541 tttaactgct tttgtttcct taggggcttt aacttcttta acttctttag cttcttttgt
      601 ttcagaagta ggggcctcaa cttctttatt agatactgag acagcattag ctactggttt
      661 agtttctgga gctttttcag atgttgttgt tggacttgca actgcttcag tttttggttg
      721 tgcttctgta tttgtaccac ctgtttcttc agctgctgct tgtgcttcgc catttgacat
      781 taataataaa agtgtactaa tcgctacaga tgcaacgcct agtgatgact ttctaattga
      841 ataaaatgat ttaaattctt tttgctgttt gttcatgttg tagaaacaac tcctaattgt
      901 atattatcaa ctgataatca ttatcaattg attacttgta ttgtacattg aaatggtagt
      961 taattgcaac attattttat aaaaaaataa cctatgttat agattatgaa atatctatga
     1021 cataggttaa ataaaattta aaataaaaaa tatattaagt tatgagtgtg acgatgattt
     1081 atttagattc ttttcttttg aaaagtaata aagataatga acctaaaagg gcaagtgttg
     1141 cgaaggcaac tgtgctaata aagttatcaa ctgaagttaa accagttttt ggtaattctt
     1201 tagctttaga tgcttgttta ggcgtttcgt tatgttttgt aactttgtta gtttgttgtg
     1261 atttattatc acttacagct tgattgttgc tttcagattt cgctgttgca acatctttaa
     1321 caggtgtttg aactttattt tgttcttgag cagtttgtgc tgttttaact gtatgagcag
     1381 tttgtgtttt agtttgatct tttgttgtgt cagtacttac aactttagta gagtgattgt
     1441 cttcaacttt gcttgttgta gttacagtag gtttaacttt ttcaacttta ggttgaactg
     1501 gtttagtttg ctcagtaggt gttttaggtt gagctggttt tggttgaacc ggtttaacat
     1561 tgtttggttt tgctgcgtca gctaatgtag gaattgcttt ttcaaattcc aaatgcgtag
     1621 tatatctatg attgtaatta atttgtggca cgacaatatg tactttagta gttaagctct
     1681 tatatccagg ttcaactgca acattgattg ttctagtatc cgctttttta ttatcgttaa
     1741 caacagttgt tgctaattct tgattgtttg cattgtaaaa tttgtattct ttccagaatg
     1801 atgcattgtt taacacggtt tggaaataat atttattatt ttgtttaatt actttaccag
     1861 ggtgttgcat atagtcatcc atgtgtgact tctctgaaga gccatctttt tgcacttgga
     1921 aattaattgg ttgtgatgtt gcttgagaaa cttgtgtgct ttgattatta gttgcgttcg
     1981 tagcttctgt tgccgcattg acttgttggc tgtctgcgcc tatgtataca agggaaccta
     2041 aaatgataga tgctgtaccc attgtaatct ttttcatagc tgatgaacgt tgttctgatt
     2101 gatacttact gtttaaataa tgttttgtca tgttgttttc ctcctaagga tacaaattat
     2161 gtgttgttat atgagatgat taaccattgc aattgataat ggttatcaat tatactagca
     2221 catctatttc cgtataacaa taataaattg attataaaat aaatattgac aatgataatc
     2281 attattattt atgattttac taaagactta aaagcaatca taaaaaggag gattatgttt
     2341 tgaaaaatat tttaaaagtt tttaatacaa cgattttagc gttaattatc atcatcgcga
     2401 cattcagtaa ttctgcaaat gccgcagata gcggtacttt gaattatgag gtttacaaat
     2461 acaataccaa tgacacgtca attgctaatg actattttaa taaaccggca aagtacatta
     2521 agaaaaatgg taaattgtat gttcaaataa ctgtcaacca cagtcattgg attactggaa
     2581 tgagtatcga aggacataaa gaaaatatta ttagtaaaaa cactgccaaa gatgaacgca
     2641 cttctgaatt tgaagtaagt aagttgaacg gtaaaataga tggaaaaatt gacgtttata
     2701 tcgatgaaaa agtaaatgga aagccattca aatatgacca tcattacaac attacatata
     2761 aatttaatgg accaactgat gtagcaggtg ctaatgcacc aggtaaagat gataaaaatt
     2821 ctgcttcagg tagtgacaaa ggatctgatg gaacgactac tggtcaaagt gaatctaata
     2881 gttcgaataa agacaaagta gaaaatccac aaacaaatgc tggtacacct gcatatatat
     2941 atgcaatacc agttgcatcc ttagcattat taatcgcaat cacattgttt gttagaaaaa
     3001 aatctaaagg caatgtggaa taatgagaaa tgttaaacaa attgctacaa aatctattat
     3061 agctattatt agcttatgta tacttacata tacaacaatg attggtagcg tgttggctga
     3121 tgagataaaa tatccatcag ccaaatttaa tcaacctgaa gcaaaagata aaacagaatt
     3181 aactacatca atttttgatg aaaagataaa agagaataaa gcgttagagt tactaatttt
     3241 taatcaagaa aataaaaatg taactgagga acaacaacta gttgacgaaa aggcgcaatt
     3301 gatttcagat atgactggta aaatttactt gcaagtaaag ctaaaaggtc aaatagataa
     3361 agaacaactt gtttttcaaa atgacaaaaa tgaagaattt ccttttgtta taaaagatga
     3421 aaaggatgac acaatagtaa gaattttaat tgaacagcat atggataaaa tcaatatgca
     3481 tgttaaaacg ttggctgaaa agaaaaatct agataacaaa gaaatggtgt attctattca
     3541 ttttaaagag aaaaaagtac aacatgatga tgcaaaagaa gtgccttcaa aacatcaaaa
     3601 tcaagaaaat aatcaagatc agcttaaaaa agatattgat gacaaaaaag atagtcaaaa
     3661 atcagatact aaggaaagac gtactagcct ttttactgaa aaaggattaa atgatattcc
     3721 tgtacaaaaa gataaagtgc aacaagacag taataaaaag attgaaaatg agcgacctaa
     3781 agcatcag
//
LOCUS       DAOBBH010000205         3786 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.205, whole genome shotgun sequence.
ACCESSION   DAOBBH010000205 DAOBBH010000000
VERSION     DAOBBH010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.205"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(11..99)
                     /locus_tag="P6L01_002341"
     tRNA            complement(11..99)
                     /locus_tag="P6L01_002341"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(62..64),aa:Ser,seq:gct)
     gene            complement(102..176)
                     /locus_tag="P6L01_002342"
     tRNA            complement(102..176)
                     /locus_tag="P6L01_002342"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(142..144),aa:Asn,seq:gtt)
     gene            complement(318..887)
                     /locus_tag="P6L01_002343"
     CDS             complement(318..887)
                     /locus_tag="P6L01_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HDP3031387.1"
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFK
                     VQKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIW
                     LNMHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMIS
                     KNPDQPIDYNKATLNVFEALTRYSLFEDK"
     gene            1097..1315
                     /locus_tag="P6L01_002344"
     CDS             1097..1315
                     /locus_tag="P6L01_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HDP3031388.1"
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELI
                     DDALVNKDEAAFNQYTAEYKNLEAFLGE"
     gene            complement(1396..2382)
                     /locus_tag="P6L01_002345"
     CDS             complement(1396..2382)
                     /locus_tag="P6L01_002345"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDP3031389.1"
                     /translation="MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIV
                     GKNQNTIEEVNQTYIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKF
                     TEPIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQ
                     NALKVNPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLT
                     DEDWENIEKLSNDKYRTWAWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIF
                     GDFFGVGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDRHELIRLMS"
     gene            2581..2757
                     /locus_tag="P6L01_002346"
     CDS             2581..2757
                     /locus_tag="P6L01_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkvS family protein"
                     /protein_id="HDP3031390.1"
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLP
                     EKTVINHKRYKIVE"
     gene            2772..3374
                     /locus_tag="P6L01_002347"
     CDS             2772..3374
                     /locus_tag="P6L01_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDP3031391.1"
                     /translation="MNKISKALTWFIISFIIFHLILFIMWGEHQEYWYLYTGIMLIAG
                     ISYVFYQRDIESKRLLTSIGVGIITAIILIMLQLLFSLITSNLSYSSLIKELARTGVN
                     WKWQMLVTLLFVIPCHELYMRTVLQKELTHFSIPKWIAILITAICSSSLFIYLDNWWI
                     VTFIFVAQVILSLSYEYTRRIATTSVAQIVAIILLLIFNA"
     gene            3606..3751
                     /locus_tag="P6L01_002348"
                     /pseudo
     CDS             3606..3751
                     /locus_tag="P6L01_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
BASE COUNT         1262 a          559 c          569 g         1396 t
ORIGIN      
        1 tagcaagtaa cggaaacgga gggattcgaa ccctcgcgcc gctttcgcga cctacaccct
       61 tagcaggggc gcctcttcag ccaacttgag tacgtttcca atggctccac aggtaggact
      121 cgaacctacg accgatcggt taacagccga tagctctacc actgagctac tgtggattaa
      181 tttgtatgtg tttgaatatc aagcacaaat attattataa caagattaag ttaatttgca
      241 atagatacct taaaaaaata tgcagaacat ttacactata tttataaaac attccgcata
      301 ttttaaaaat aaacaattta tttatcttca aataaagaat agcgtgtcaa tgcttcaaac
      361 acattcaatg tggctttatt ataatctatt ggttggtcgg gattttttga tatcatgcga
      421 gattgtttat ctaccatgta atagtaaaaa atggaattgt tatattgatg ccataaactg
      481 tggtatgaaa catgaagaat gatagattca ttattaataa atgtcacttt acatttacga
      541 ttttttaatt ctttaatact ttcgatataa tgcatattta accaaatatt ttcattttgt
      601 ctgtcagaat gtgttgggaa aaaataagtt ggaaataatg gtgttagtaa aataggtggt
      661 ttactagaaa tgccagtaat gcggtttgtc tctgctttct tgccaagata agtattacca
      721 taaaatttgc aagagcgttc aatgattttc tggactttaa aaggattttg aatacgcgtt
      781 ttgtcaaacc gaattatttc actaccgttt ctttgatcgt catcatcaaa tgctggtcga
      841 ataaccatgt ctcctttgcg tatcacataa atattttgag aatacataag caaaacctcg
      901 ctttattaag ttttaaacca ttaatataaa aatacaatta atgatataaa gatattttat
      961 aataaaattc tgagttgtca agttattatt ttggtgaaaa tgtacaataa ttttttacta
     1021 atagtcagaa tattcattga tttcaaaatt agaaaaatgt atacttatta taaattaaag
     1081 aaaggtagtg gtttctatga aatacaatac taatgttaaa catacaactt tagaagcgtt
     1141 tgtcacaact gtcaatgatt tgggtattga attaattatc aatgaagcac ttcgagaggt
     1201 aagaaaacga cagctcatag aacttataga tgacgcactc gtcaataaag atgaagcagc
     1261 atttaatcaa tatacggcag aatacaaaaa tttggaggca tttctcggtg aataacattg
     1321 attcgaattc cataacgcaa tcataaaagg actattctta acatctcata catgtaagaa
     1381 tagtccttta atcgtttatg acattaatct aattaattca tgtctatcta tgtcaccgaa
     1441 ataatgatat aaatcatatt ctgatagtgc ttcttctatg tgctcaaagt cgtgtagaca
     1501 gccaactaat gcattttcaa gatcagtgac atctccgaca ccaaagaaat caccgaaaat
     1561 ttttgcatgt tcgattttac ctcgtttaac atcaaactta atttgtacaa atcctttttc
     1621 aaatttttcc tcgcgttcaa agttatattt agggtttcta ccataattcc atgcccaagt
     1681 tctatatttg tcgttactta acttttcaat attttcccaa tcttcatccg ttaatttata
     1741 ttcttctact tcagtttctc caaagatagt tttcaagatg atttttttaa attcttcaat
     1801 ttctaaaggg tcatttaaaa attcttgaat gtttgctact cgtttacgaa cagatttaat
     1861 accttttgat ttaatcttag ctggattaac ttttagtgca ttctgaactt cgtctaaatc
     1921 actatttaac atcaacgtac catgactaaa cattctattt ttaactttaa ccatagcatt
     1981 ccctgagatt ttcgcttgtc caacttgaat atcgttacga ccagttaatt cagcatttac
     2041 gcctaaagat tgtaatgctt gaacaattgg ttcagtgaat ttttggaaat tgtggaaact
     2101 gttaccatca tcatctgtta taaaactaaa gtttaaattg ccagtatcat gataaacagc
     2161 gccaccacca gaaattcttc taactacatc gatgttatga gcatctatat atgtctgatt
     2221 tacttcctct atcgtatttt gattctttcc aacaatgata gatggtctat ttatgtaaaa
     2281 taaaaagtaa ctttcttctg ctggtaaatt ttttaaaaca tattcttcca ttgctaagtt
     2341 taaagttgga tctgtaatat tattattact aatgaatttc attacaatct ctcccttata
     2401 tctatatata ttctctactt atttatgcct taactttgct caaaatgcca attatatact
     2461 cacaaatcat tctaaatatc ggcttaaagt tacattttat tatttttgat tgttaatagt
     2521 ttatttgtta agtgaatttt agtacaatat aatttagtta tgatttaagg aggacttaaa
     2581 atgactgttg cagaagtggg taacattgtt gagtttatgg atggattaag aggtcgtgtt
     2641 gaaaaaatca acgataactc tgttattgtt gacttaacaa ttatggaaaa ttttaatgac
     2701 cttgatttac cggaaaaaac tgttatcaat cataaacgat ataagattgt tgaataagaa
     2761 ggtaagttat aatgaataaa atctcgaagg ctttaacttg gtttattata agtttcatta
     2821 tatttcatct catattattt attatgtggg gcgaacacca agaatactgg tatttatata
     2881 caggtataat gctaattgct ggtatcagtt atgtatttta tcaaagagat attgaatcta
     2941 agcggttgct tacatcaatt ggtgttggta ttattacggc aattatttta attatgcttc
     3001 aacttttatt ctcacttata acttctaatt taagttatag ttcattaatt aaagaattag
     3061 caagaacagg tgtcaattgg aagtggcaaa tgttagtaac tttacttttt gtcattccat
     3121 gtcacgagtt atatatgaga actgttttac aaaaagaatt aacacatttt tcaataccga
     3181 aatggatagc tatattaata actgcaattt gttctagttc attatttatt tatttagata
     3241 attggtggat agtaacattt atatttgttg cacaagtgat attatcatta agttatgagt
     3301 atactcgacg catagcaaca acatccgttg cgcaaattgt tgctattata ttgttattaa
     3361 tttttaatgc ttaaataagc ttttcttaga cgtctgtgtt ctaagaaaag ctttttgctt
     3421 tagaaaagca atacgaagat tcaaggcatg ctttattata gtgtatacat ttttaacggc
     3481 aagcagttat tattgaggtt atattttagt cagtttttat tactaaatat tttataaaaa
     3541 tagtataata ttttgtgggc aagttgaatt gatggtggct atctgagtaa aggggggtgg
     3601 tacctatggc attacttaac acttttgaaa ggaaaagcct attgtgatat ctattgcaaa
     3661 cgcattacat ttaatgttaa gtttcggtat gtttatcgtc actttcattg gtatagtagt
     3721 agcaataata aatttaagca ataaaaaata accatcattt caaaactttg acatagaatg
     3781 atggtt
//
LOCUS       DAOBBH010000206         3784 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.206, whole genome shotgun sequence.
ACCESSION   DAOBBH010000206 DAOBBH010000000
VERSION     DAOBBH010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.206"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..203)
                     /locus_tag="P6L01_002349"
     CDS             complement(<1..203)
                     /locus_tag="P6L01_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDP3031392.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWF"
     gene            complement(627..1064)
                     /locus_tag="P6L01_002350"
     CDS             complement(627..1064)
                     /locus_tag="P6L01_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HDP3031393.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAP
                     SSPSYALFKGQDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
     gene            complement(1080..2204)
                     /locus_tag="P6L01_002351"
     CDS             complement(1080..2204)
                     /locus_tag="P6L01_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HDP3031394.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEETQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEVILPDIKAAFSDANKVLESVNEPQIDNYFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTAHDNIVFMRKWLDLGN
                     RYGNIEEVMDGVLEEVLATYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPAVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMANRTI"
     gene            complement(2242..2493)
                     /locus_tag="P6L01_002352"
     CDS             complement(2242..2493)
                     /locus_tag="P6L01_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HDP3031395.1"
                     /translation="MKIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            complement(2505..2699)
                     /locus_tag="P6L01_002353"
     CDS             complement(2505..2699)
                     /locus_tag="P6L01_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HDP3031396.1"
                     /translation="MSRILTFSEQNALAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            2944..3648
                     /locus_tag="P6L01_002354"
     CDS             2944..3648
                     /locus_tag="P6L01_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HDP3031397.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTALFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
BASE COUNT         1161 a          648 c          570 g         1405 t
ORIGIN      
        1 atgaaccata ataattcggt tgctactaat ttaaaagaaa ctttctttgt cgttaatagt
       61 ggaaatcctt tagataagtc aaagcgaagt tgatgaccaa atttcgaaat cgtacctgta
      121 tttgtgcgat catttcgtgt atttcctatt tctaaaacct cttcacaaag actgtgatat
      181 gctgcatcaa atgaattcaa catatgcgat aacacctcat tttcattatt tatagtatgt
      241 atatttagtt tgatataact taattttatg tagcattttg ttatcactca ttttaggaat
      301 atgatattaa tatcatgaat tccgttactt tatttataaa atgctgatta agtacctacc
      361 acatcgtaac gtgatatatg tttccaattg gtaattgttt acccaaatct ataactttaa
      421 tgctaaaaaa ttttaaaaaa gaggttaaca catgatttga atattatgtt tgatgtccta
      481 ttaaaacagt taaatttcta gaaaatatag ttggtaaaaa cggactttat ttaacaaata
      541 gaatacaact atattctcta ttttcaatga cagacaccat ttttaatatt ataaaatgtg
      601 ttaaccttta tatttattta tgtgtactat ttacaatttt cgtcaaaggc atcctttaag
      661 tccattgcaa tgtcattaat atctctacct tcgataaatt ctctaggcat aaaataaact
      721 aaatcttgac ctttgaataa agcatacgaa ggactagatg gtgcttgctg aatgaattct
      781 cgcattgtag cagttgcttc tttatcttgc ccagcaaaaa ctgtaactgt atttgtaggt
      841 ctatgttcat tttgtgttgc aactgctact gcagctggtc ttgctaatcc agctgcacag
      901 ccgcatgtag agttaataac tacaaaagta gtgtcatcag catttacttg gttcatatac
      961 tccgatactg cttcgctcgt ttctaaactt gtaaaaccat tttgagttaa ttcgccacgc
     1021 atttgttgcg caatttcttt catataagca tcatatgcat tcatatttaa ttcctccaat
     1081 taaattgttc tgtttgccat ttgtttccat actgaaccaa gtgcctcatc tccgttttca
     1141 atacgagata tggccatttc aatttgtaat ttaacttcaa acgcattgtc attaatatgg
     1201 gcttttagtg cgggaagctg ctctgcatta ccttcatcaa agataaacat agcagcacgc
     1261 cacctaacga ttttctgtgg atcatctaat agtagcacca tttctggtag tgcctctgga
     1321 taccctaaat cgcttatgca atcccctgct gttcttctta cagcaggact tttatcacga
     1381 agccccttat ataaatacgg taaaatttct ttactttcaa tcatacctaa taatacaatc
     1441 gcttgacgtc taaccggtac tttttcatca gataaagcta aatcaagtag cggtatatct
     1501 tcaaaagtcg gctttggaaa atggtttaac attcgtaatc gagtcttcca attatcagtt
     1561 gcatgatatt catccaaaga gacatgtcga tagaaatgat aattattagt tgcgtgattt
     1621 tcttctaaag catgttttac caatacgggt aactgtgatt ctggataggt agctagcact
     1681 tcttctagga caccatccat tacttcttca atatttccat agcgatttcc taaatctagc
     1741 catttacgca taaaaacaat attgtcatgt gctgtttgcg cttgagtcat atgatcaaca
     1801 tatgtttgtg gtaattgttc tcttaattct tggtcagcag aagttagctt aatttgatac
     1861 ggtataccct taaaagttaa taattcagct ttaatttcac caaaataatt gtcaatttga
     1921 ggttcattta cagattctaa aaccttattc gcatcagaaa aagcagcttt aatatcaggt
     1981 aatatgactt cccaatcagc ttttggtgcc ttatcaacag ctaagaagtt catgacatga
     2041 aaaatggaag tgataccatc tatagataac aactgattta taaatcttgg ttgtgtttct
     2101 tctacttttt tataagtatt agataacttg tcttctcttg tatatgacaa aacaactttc
     2161 attgtatttg gacttggtgt tggctctata cgtaaaattt ccatatactt tattcccccc
     2221 gaatactagt tgatgaaaaa tttattcgaa tacagcctct acttttggca atactgtttc
     2281 ccaatttgcg tcattttctt tatctactga aataaagtcc ataacatgga aaattgattt
     2341 aacgccttca acctttaaga tgtcattaat aaatgctggc tgtgaatcat caactttagt
     2401 atacgtatct gatgtcatac cttctctgct ttcactaagt gtaatcttca ttgtgttgtg
     2461 gttcggtgtt tctgatatag atataatttt catttgactt cctcctattg aagttcattt
     2521 cctaattgtt ttatttcttt ccctattgaa cactcattga tacaaaaatg atgcgccttc
     2581 gttttcccct ctaatttacg cagacgagtt ttgattggac attgattgca ataagtattc
     2641 attaaatcat ctattttcgc gagtgcattt tgctctgaaa aagttaaaat acgcgacact
     2701 tcctttatta cttcaaaata ttgcaacctt gtaaataagt gttactgttt taaatttgac
     2761 ataaaaatat tataacattt ttgaccctaa gatttgaatg aaacctttta caattctata
     2821 cgtaaaatgt atttatattc aaatgctatt gcatcgcaaa cgatttttca gtaaaatgag
     2881 taagttattt attgagcggt ttctaaacac ccgcatgatc aaaatttagg aggacatttt
     2941 aatatgtata atgaaatttt aggactagtt acgtttattg caacgttcgt acttatggta
     3001 cttatgtatc gcttttttgg taaacaaggt ttaattgcat gggttgcaat tggcacaatc
     3061 attgccaaca tacaagtgat taaaactgta gaaatctttg gtatttcagc aactttaggt
     3121 aatgtcatgt ttgcttctat ttatttagca acagatattt taaatgatat ttatgggcgt
     3181 agagttgcaa aaagagcagt ttggttaggc ttttcatcaa cattaattat gattattgtt
     3241 atgcaattgt cattacattt tattcctgca ccagaagata tggcacaaaa agcattacac
     3301 gcaatctttg atgttgtgcc acgtattgct ttaggctcaa ttgtcgcata tattattggt
     3361 caacatattg atgtatttat cttttcacta attaaaaaag tatttagttc tgataaaacg
     3421 tttttcatcc gtgcatatgg tagtacattt ttaagctcaa taattgatac agctttattt
     3481 gtagctatcg cttttatcgg aagtttacct ggtacagttg tatttgaaat atttattaca
     3541 acttacgtat taaaattagc ttcaacagtt ttcaatgtac catttggata tattgctaaa
     3601 tcattttatc gtaaaggtaa gattgataag ttagatcaag gctattaacg cacttactac
     3661 acaaaagaat aagttttaag ggagtgggac agaaatgata ttttcgcaaa atttatttcg
     3721 tcgtcccacc ccaacttgca ttgtctgtag aaattgggaa tacaatttct ctttgttggg
     3781 gtcc
//
LOCUS       DAOBBH010000207         3745 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.207, whole genome shotgun sequence.
ACCESSION   DAOBBH010000207 DAOBBH010000000
VERSION     DAOBBH010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.207"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            46..816
                     /locus_tag="P6L01_002355"
     CDS             46..816
                     /locus_tag="P6L01_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDP3031398.1"
                     /translation="MDLNLTMIIIIILFGFIAAFIDSVVGGGGLISTPALLAIGLPPS
                     VALGTNKLASSFGSLTSMIKFIRSGKVDLFVVAKLFGFVFLASACGAYIATMVPSQIL
                     KPLIIIALSSVFIFTLLKKDWGNTRTFTQFTFKKAIIFAALFILIGFYDGFVGGGTGS
                     FMLFVLLVFGFDFLSAAGNAKVLNFASNIGALVLFMVLGQVDYVIGLIMAISMIAGSY
                     TGAHFAIKQGVGYVKVLFIIITAILILKNAFDYIQQFV"
     gene            914..1408
                     /gene="tpx"
                     /locus_tag="P6L01_002356"
     CDS             914..1408
                     /gene="tpx"
                     /locus_tag="P6L01_002356"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDP3031399.1"
                     /translation="MTEITFKGGPIHLKGQQINEGDFAPDFTVLDNDLNQVTLADYAG
                     KKKLISVVPSIDTGVCDQQTRKFNSEASKEEGIVLTISADLPFAQKRWCASAGLDNVI
                     TLSDHRDLSFGENYGVVMEELRLLARAVFVLDVDNKVVYKEIVSEGTDFPDFDAALAA
                     YKNI"
     gene            1531..2478
                     /locus_tag="P6L01_002357"
     CDS             1531..2478
                     /locus_tag="P6L01_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDP3031400.1"
                     /translation="MAEQQTIMERLFHTLDEKAKTLNNENGQSFIENLGLAMEQVYTN
                     ERGLLEQSTLQDRRKAFQFAYLSLMQEEKIQANHQITPDSIGLILGFLVERFMNNQEE
                     LHIVDIASGAGHLSATVKEVLPEIAVMHHLIEVDPVLSRVSVHLANFLEIPFDVYPQD
                     AIMPLPLEEADIVIGDFPVGYYPIDERSKEFKLGFEEGHSYSHYLLIEQAINALKDAG
                     YAFLVVPSNIFTGEHVKQLEKYIATETEMQAFLNLPPTLFKNEKARKSILILQKKKSG
                     ETKPVEVLLANIPDFKNPSQFQGFMTELNQWMDTNRPKK"
     gene            2566..>3745
                     /locus_tag="P6L01_002358"
     CDS             2566..>3745
                     /locus_tag="P6L01_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDP3031401.1"
                     /translation="MSKLILAINAGSSSLKFQLIRMPEEELVTKGLIERIGLKDSIFT
                     IEVNGEKVKTVQDIKDHVEAVDIMLDAFKAHNIINDINDIDGTGHRVVHGGEKFPESV
                     AITDEVEKEIEELSELAPLHNPANLMGIRAFRKLLPNIPHVAIFDTAFHQTMPEKAYL
                     YSLPYHYYKDYGIRKYGFHGTSHKFVSQRAAEMLDKPIEDLRIISCHIGNGASIAAID
                     GGKSIDTSMGFTPLAGVTMGTRSGNIDPALIPFIMEKTGKTAEQVLEILNKESGLLGL
                     SGTSSDLRDLSEEAESGKARSQMALDVFASKIHKYIGSYAARMHGVDVIVFTAGIGEN
                     SVEIRAKVLEGLEFMGVYWDPKKNENLLRGKEGFINYPHSPVKVVVIPTDEESMIARD
                     V"
BASE COUNT         1287 a          518 c          720 g         1220 t
ORIGIN      
        1 aaataaaatc ctagagtaaa tttaaacata aggggatgtt aaactatgga tttgaactta
       61 acgatgatta taatcataat tttatttggt tttatcgcgg cgtttataga ttcggttgta
      121 gggggtggcg gtttaatttc tacgccagca ttattagcaa tcggtctacc accatctgtg
      181 gctttaggta caaataaatt ggcaagttca tttggttctt taactagtat gataaagttt
      241 ataaggtccg gtaaagtgga cttatttgtt gttgccaaat tatttggttt tgtatttttg
      301 gcatctgcat gtggcgcata tattgcaacg atggttccgt cacaaatatt gaaacctcta
      361 atcattattg cactttcgtc ggtgtttata ttcacattac ttaaaaaaga ttggggcaat
      421 acacgcacgt ttactcaatt tacatttaag aaagccataa tatttgcagc actttttata
      481 ttaatcggct tttatgatgg atttgtaggt gggggaacag gttcatttat gctttttgta
      541 ttgttagtct ttggttttga ttttttaagt gcagcaggaa atgctaaagt tttgaacttt
      601 gcttctaata taggtgcgct tgtattattt atggtattag gacaagtaga ttatgtaata
      661 ggtttaatta tggctataag tatgattgct gggtcatata ctggagcaca ttttgctatc
      721 aaacaaggtg ttggctatgt aaaagtatta tttattatta ttactgctat tttaatttta
      781 aaaaatgctt ttgattatat tcaacaattt gtataaaatt aaatttgctt ttgattaaag
      841 catgaaaatt gtaatcaaac cataaattgt cgtatgatgt agttagaatt ttaaaatgca
      901 ggaggtcaag tatatgactg aaataacatt caaaggtgga ccaatccact taaaaggtca
      961 acaaattaat gaaggtgatt ttgcacctga ttttacagtg ttagataatg acttaaatca
     1021 agtaacatta gcagattatg ctggtaaaaa gaaattaatt agtgtggtac catcaattga
     1081 tacaggtgtt tgtgatcagc agactcgcaa attcaactct gaagcttcta aagaagaggg
     1141 gattgtgctt acaatttcag cagacttacc attcgcacaa aaaagatggt gcgcttcagc
     1201 aggtttagac aatgtcatta cattaagtga ccaccgtgac ttatcatttg gtgaaaacta
     1261 tggcgttgtt atggaagaac ttcgcttatt agctcgtgca gtatttgtat tagatgtaga
     1321 taataaagtt gtttataaag aaatcgttag tgaaggtact gatttcccag attttgatgc
     1381 tgctttagct gcatacaaaa atatttaatc attaaagaga taaatcttaa aatgtataca
     1441 tcgtgtccat cgttgtcaac agcattaaaa tagaattgtt ttctatgatt gctaagacct
     1501 atgggcactt tttattggag agggacgaat atggcagaac aacaaacaat tatggaacgc
     1561 ttgtttcata cattagatga aaaagctaaa acattaaata atgaaaatgg ccaaagtttt
     1621 attgaaaatc ttgggctagc aatggaacaa gtatatacca atgaaagagg attgttagaa
     1681 caatcgacgt tacaagatcg acgtaaagca ttccaatttg catatttaag tttaatgcag
     1741 gaagaaaaga tacaagcaaa tcatcaaatt acaccagatt caattggatt gatactagga
     1801 tttttagttg agcgttttat gaacaaccaa gaagaattac atattgttga tattgcaagt
     1861 ggtgccggtc atttaagtgc tactgtaaaa gaagtgttac ctgaaattgc ggttatgcat
     1921 catttaattg aagttgatcc agttttatca cgtgttagtg tacatttagc aaacttctta
     1981 gaaattcctt tcgatgtgta tcctcaagat gccatcatgc cactaccatt agaagaagca
     2041 gatatcgtta ttggtgattt tccagtaggc tattatccaa ttgatgaaag aagtaaggag
     2101 tttaagctag gttttgaaga aggacatagt tattcacatt atttattaat agaacaagca
     2161 ataaatgcat taaaagatgc tggatatgcc tttctagtgg taccaagtaa tatttttaca
     2221 ggtgaacatg taaaacagct tgaaaaatat attgcaacag agacagagat gcaagcattt
     2281 ttaaatttac caccaacttt atttaaaaat gaaaaagcgc gaaaatctat attaatttta
     2341 caaaagaaaa aatcgggtga aacaaagcca gttgaagtat tattggcaaa tattcctgat
     2401 ttcaaaaatc cttcacaatt tcaaggattt atgacagagt taaatcagtg gatggacaca
     2461 aatcgtccta aaaaataatc tgtaatagta tagtcataaa actgtatgaa tgataaaatg
     2521 agaaatgaaa tagtataaaa agcaaacgat ggaggatcat tacttatgtc aaaattaatc
     2581 ttggctatca atgctggtag ttcatcatta aaatttcaat taattagaat gcctgaagag
     2641 gaattagtaa caaaaggttt aatagaaaga atcggattga aggattcaat ttttacaatt
     2701 gaagtcaacg gggaaaaagt taaaacagta caagatatca aggatcacgt tgaggcagtt
     2761 gatatcatgt tggatgcttt taaagcgcat aatattatta atgatattaa cgatatcgat
     2821 ggaacaggtc accgtgttgt acatggtggt gaaaaattcc ctgaatcagt agctattact
     2881 gatgaggttg aaaaggaaat tgaagaatta agcgaattag caccgcttca caacccagct
     2941 aacttgatgg gtattcgtgc tttccgtaag ttattaccaa atatccctca tgtggcaatt
     3001 tttgatacag cattccatca aacaatgcct gaaaaagcat atctatatag cttgccatat
     3061 cattattata aagattatgg cattcgtaag tatggtttcc atggtacaag ccataaattt
     3121 gtatcacaaa gagcggcaga aatgttagat aagccaattg aagatttacg tattatttca
     3181 tgtcacattg gtaatggagc atcaattgca gctatcgatg gcggtaaatc tattgataca
     3241 tcaatgggct tcacaccact tgctggtgta acaatgggta ctcgttctgg taatattgac
     3301 cctgctttaa ttccatttat tatggaaaaa acaggtaaaa ctgctgaaca agtattggaa
     3361 atcttaaaca aagaatcagg tttattagga ttatcaggta catctagtga tttacgtgac
     3421 ttatcagaag aagctgaatc aggtaaagca cgttcacaaa tggctttaga tgtatttgct
     3481 tctaaaattc acaaatatat tggatcatat gctgcgagaa tgcatggtgt tgatgttatc
     3541 gtatttacag caggtatcgg tgaaaactca gtagaaattc gtgcgaaagt tcttgaaggc
     3601 ttagaattta tgggtgttta ctgggatcct aagaaaaatg aaaacttact acgtggtaaa
     3661 gaaggattta ttaactatcc tcattcacca gttaaagttg tcgttattcc aacagatgaa
     3721 gaaagtatga ttgcccgtga tgtta
//
LOCUS       DAOBBH010000208         3645 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.208, whole genome shotgun sequence.
ACCESSION   DAOBBH010000208 DAOBBH010000000
VERSION     DAOBBH010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.208"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            1..1566
                     /locus_tag="P6L01_002359"
                     /pseudo
     CDS             1..1566
                     /locus_tag="P6L01_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
     gene            1569..1889
                     /locus_tag="P6L01_002360"
     CDS             1569..1889
                     /locus_tag="P6L01_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDP3031402.1"
                     /translation="MKFIIAILLGLIVSITIAFTIIHHPILDRFNPFLKTEYSYAKVP
                     KGTQQYVNITAYSERGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISITYVEKEDIPKE
                     VRQE"
     gene            1886..2527
                     /locus_tag="P6L01_002361"
     CDS             1886..2527
                     /locus_tag="P6L01_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3031403.1"
                     /translation="MIELKDLTIQKGNIHILKKLNLKFQCGKSYALIGKSGCGKSTLL
                     NTIAGLEKTGKQYVYFNGQLEQFKSNFYRDKLGYLFQNYGLIDNLTVNENLDIGLAYK
                     KISKKEKEQLKIRYIEQFGLSNSLKRKVHTLSGGEQQRVALIRMMLKDPIVMLADEPT
                     GALDPKTGQMIIQSLFDLVDENKVLILATHDMAIANQCDEIIDLEQYSKVASM"
     gene            complement(2615..2905)
                     /locus_tag="P6L01_002362"
     CDS             complement(2615..2905)
                     /locus_tag="P6L01_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031404.1"
                     /translation="MFYACHFKMKIGGVILTRTYNIIGILSCLISFIIMALPMIWYTA
                     SALWFFPGAIMILLLSLVIVFCYIKTKNQLHLLLIVLNIIILLFFSLPLLLS"
     gene            3046..3183
                     /locus_tag="P6L01_002363"
     CDS             3046..3183
                     /locus_tag="P6L01_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001788572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(glycerol-phosphate)
                     alpha-glucosyltransferase"
                     /protein_id="HDP3031405.1"
                     /translation="MADKILKLVNNDVLAEEFGSKARENIIEKYSTESILEKWLNLFN
                     S"
     gene            complement(3272..3628)
                     /locus_tag="P6L01_002364"
     CDS             complement(3272..3628)
                     /locus_tag="P6L01_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDP3031406.1"
                     /translation="MKLGMLLIRWMLATSFLVHGTLKFVDLDGTIHFLGSLGLPPTIA
                     VLMSIGEVVGGLALIIGFLTPYVNVFYICVMLGSIFTLKLTRGYVSGYEFEIIHIVMN
                     LACISSYSWKKWLQFY"
BASE COUNT         1436 a          459 c          566 g         1184 t
ORIGIN      
        1 taaatttttt aaaagaatat ggtgttactg ctgaatcata caaaatagat catatgatga
       61 ttgccgttgg tgtcattaaa caaatgaata tagaagttcc gttatccgca cttttaatcg
      121 tttattttat ttattatatt ttcgaaaaga acattaattt caaagcgtat gcgattaagt
      181 atttaaatgg ttttacatta agaaaaaata atttttgaaa atttttcaaa aaaatgcacg
      241 tattgggtaa cgttaatcat aagtcaaatt cttttaacta caagtgtact ttggatatta
      301 aattacacgg gtaatttaga tttattcata ttaagattag ttctgctttc atgtcttttc
      361 attttaacga ttagtgttat taatttatgg actttcttaa tgttactaaa tttaaatatt
      421 gctaatatga ttaaaggtaa gcagcacttt aaaacaattc gttttattaa tacagtttgt
      481 aaaagtattc tcttagtact aatagctagt gtaatgatag aaaatactag tgttattaaa
      541 gatttgaata aaataaagga aactgagaaa tattggaatg tattagatga ttattacacg
      601 attgaatttg caccttatca cgaaacgaaa caaagtttga ttgataatat gctgcaatca
      661 gaacaattag taaaggctag tgaagcagaa aataatgcga ttttattcaa accaaagggt
      721 gactccgttg acaatgacaa cttttcgcct gatgagggga atgtaatatt agtaaataat
      781 cagttctggt cgatttatta caagcagttt caacctgata ttccgataaa aaatcaaaaa
      841 aataatgtcg aagtaattat tccacaaaag tttcatgcaa tgcgtaatga aatcaatcaa
      901 gcatatcatt catggtttga atttgtacaa aataaaaata ataaagagaa taagttatct
      961 atacagttta tcaacaaaaa tgattatcga attttttcat ttgatgcacg agatagtcgc
     1021 catttgtcat ttatagaggc gccaatcatt gtgaatgttc aggcatcaga tttatcgaat
     1081 gatttttatt atgccatgat cagtcaaggc gggtatttat tcaaaaatta tgacgcgcta
     1141 gtaaaaaata ttgaaaatta tcatcttgat ggggaaatca gtggaataac caattataaa
     1201 gatagcgtga tggaaatgta tcatgaaaac aatttgaaat taacagtact caacttttca
     1261 caaatcatta tcgcaatcat tttaataatt attattttat ttgatgtgaa atattatttt
     1321 gaacagcatc gaaaattact cgtaatcaaa aagctatatg gttattcaac attaagagcc
     1381 aattaccaat acttattaat aaataatata gttgttgttt ttattggaat attgacgaat
     1441 gtaattttac attctcagta tataatgatg atatttgcaa cgattcttgt cgttcaaata
     1501 ttattgcaga tttgcagcct atactatcat ggccgacgtt ttaatgaagt tatcaaggag
     1561 ttttaactat gaaatttatc attgcaatat tattaggatt aatcgtatcc attactattg
     1621 cttttacaat catacatcat cctatacttg atcgttttaa tcctttctta aaaacggagt
     1681 atagttatgc caaagtgcca aaaggtacgc aacaatatgt taatattacg gcttatagtg
     1741 aaagagggga aaagcttgat tataaattaa catttaatgg attttcacct agtagaacgt
     1801 atgtcgaaat aaagcataaa gggcaatatg tcatatcgat cacatatgtt gaaaaagagg
     1861 atataccaaa agaggtaaga caagaatgat agaattaaaa gatttaacca tacaaaaagg
     1921 taatatacat attttgaaga aacttaattt gaaatttcaa tgtggaaaat catatgcact
     1981 tattggtaaa agtggatgcg gtaaatctac attattaaat actattgctg gacttgaaaa
     2041 aacggggaaa caatatgtct attttaatgg tcaattagaa caatttaaat ctaattttta
     2101 cagagataaa ttaggatatt tatttcaaaa ttatggatta atcgataatt tgacagtaaa
     2161 tgaaaattta gatattggat tagcatataa aaaaataagt aagaaagaaa aagaacaatt
     2221 aaagatacgt tatatagaac agtttggtct gtcaaacagt ttaaaaagaa aagttcatac
     2281 gctaagtgga ggtgaacaac aacgtgtcgc tttaattaga atgatgttaa aagatccgat
     2341 tgttatgtta gctgatgaac caacgggtgc gttagatcct aaaacaggac agatgattat
     2401 tcaatcatta tttgatttgg tcgatgaaaa taaagtgctg attttagcaa cacatgatat
     2461 ggctattgca aatcaatgtg acgaaataat agatttagaa cagtatagta aagtagcatc
     2521 tatgtgatat ccaaaagata aaattaaaag ccaatcaatg ggaaatgttg acttcaacac
     2581 tccgtttgat tggcttttat aatgtgttac taagttatga aagtaacaac ggtaaactga
     2641 aaaaaagcaa tataataata tttaatacga ttagtaataa atgtaattga tttttcgttt
     2701 taatatagca aaaaacaatg actaaagata acaacaatat catgattgca cctgggaaga
     2761 accataatgc tgaagctgtg taccaaatca ttggtaacgc cataataata aaagatataa
     2821 gacaagaaag gataccaata atattataag ttctagtcaa aattacacct ccaattttca
     2881 ttttaaaatg acatgcgtaa aacaatcatt ttaaatacat catacaataa aattaaattg
     2941 tgcggtagaa gaatttgaaa ttaaattgaa ttggcttgat taaaaggtga ttttatagaa
     3001 gttaataaaa atggttattt aatagaaaaa cataatatta aagacatggc tgataaaata
     3061 ctaaagcttg ttaataatga tgttttagca gaggagtttg gttcgaaagc gagagaaaac
     3121 attatagaaa aatattcaac ggaatcaata ttagaaaaat ggttaaatct tttcaatagc
     3181 taaatgaaaa aatcctaggt attcatgagt acctaggatt tttacgcatg atatatacaa
     3241 aaaaagagag gatagtcctc tcaaataata gttaataaaa ttgtagccat ttcttccagc
     3301 tgtaactact aatacaagct aagttcatta cgatatgtat gatttcaaat tcataaccac
     3361 ttacatatcc tcttgtaagt ttaagtgtga agattgagcc taacataaca caaatataaa
     3421 aaacattaac ataaggcgtt aaaaagccga taataagtgc taaacctcca acaacttcac
     3481 caattgacat caatactgcg atggtaggtg gtaaacctaa agagcctaag aagtgaattg
     3541 ttccatctaa atcaacaaat ttcaaagtac catgcactaa gaatgatgtt gctaacatcc
     3601 atctaattaa aagcatacct aatttcatca tgtcacctcc aacat
//
LOCUS       DAOBBH010000209         3645 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.209, whole genome shotgun sequence.
ACCESSION   DAOBBH010000209 DAOBBH010000000
VERSION     DAOBBH010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.209"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(217..2694)
                     /locus_tag="P6L01_002365"
     CDS             complement(217..2694)
                     /locus_tag="P6L01_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDP3031407.1"
                     /translation="MSDPTLFDYSMIKGTVEAILFQNSDNFYTVLKVDTIETNEDFDT
                     MPTVVGFLPNIVEGDVYTFKGQVVDHPRYGKQLKAETFEKEMPQTKEAIISYLSSDLF
                     KGVGKKTAQNIVNTLGDNAINDILDDHSVLEKVSGLSKKKQKQIAEQISANQESEKIM
                     IRLHDLGFGPKLSMAIYQFYLGDTLTILDRNPYQLIYDIKGIGFNKADQLARNIGIAY
                     NDNERLKAALLYTLEEECIKQGHTYLPINVVIDLTVDVLNYQDEEVIEPEKLDEMLQY
                     LNEEKRLIIDNEQVAIPSLYYSEIKSVQNLFRIKTHTNKLTEIEQSDLQMHIGEIEDA
                     NQVNYAASQKEALQTAINSKVMLLTGGPGTGKTTVIKGIVELYAEIHGLSLDYDDYVN
                     DDYPVVLAAPTGRASKRLQESTGLEAMTIHRLIGWNQDTKPEDILENEINARLIIIDE
                     MSMVDTWLFHQFLSAVPLDAQLIFVGDEDQLPSVGPGQVFKDLIESKAIPRVNLTEVY
                     RQQDGSSIIELAHRMKLGQKIDITQRFHDRSFINCQANQIPTVVEKVVTSAVNKGYTM
                     ADIQVLAPMYKGNAGIKRLNQVLQDILNPKKKDTREIEFGDVVFRKGDKVLQLVNRPN
                     DNIFNGDIGVIVGIFWAKENALNKDVLVVDFEGNEITFTKQDMMELTHAYCTSIHKSQ
                     GSEFPIVIMPIVKQYFRMLQRPILYTGLTRAKTSLVLLGDPEAFDIGLKTNGQARLTQ
                     LCTLLKNYFNSEDEEMLENTATNDTGASQTTIDDQVEAPMSSNDSGEVTSDSTKTDIN
                     VLTEATMFKIDPMINMGEITPYDFIER"
     gene            complement(2696..3364)
                     /locus_tag="P6L01_002366"
     CDS             complement(2696..3364)
                     /locus_tag="P6L01_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDP3031408.1"
                     /translation="MIDQQTIYQYIQNGKIEEALQALFGNIEEDPTIIENYINAGIVL
                     ADANEIEKAERFFQKALTIDPKNGVVFYNLANVYYNQQRYQEAIKLYQQALQTEIEQV
                     DCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQL
                     KHVLTIDKNHVDARYNLGLALFMKNEDIDEAITHFKEAVTIDPKHLLSQHALKTFTKM
                     KEEE"
BASE COUNT         1115 a          686 c          493 g         1351 t
ORIGIN      
        1 tcctcaaaag ggacgaacgt tatcgcggta ccaccctagt tataaatgca attcaacaca
       61 tttatcactt taaatcgact atacagttgt gcataaagta gcgttcacta atgtttgttg
      121 tacttttcac caaccagtac atctctgata aacaaatcat taactactca tctttatacg
      181 aatttaattc tattttagtt acatttacgc ttgttgtcaa cgttctataa agtcatacgg
      241 cgtgatttct cccatattaa tcattgggtc aattttaaac attgtagctt ccgttaatac
      301 atttatatct gtttttgttg aatcagacgt aacttctcca ctatcattcg atgacattgg
      361 cgcttctact tgatcatcta ttgtcgtttg tgaagctcct gtatcattag ttgctgtgtt
      421 ttccagcatt tcttcatctt ctgaattaaa ataatttttc aacaatgtac ataattgtgt
      481 taaacgcgct tgaccatttg tttttaatcc aatatcaaat gcttctggat caccaagtag
      541 aactaaactc gttttcgctc tagttaaacc agtatataat atcggtcttt gtaacattct
      601 aaaatactgt ttaacaatag gcatgataac aataggaaat tctgaacctt gtgatttatg
      661 gattgatgta caataagcat gtgttaattc catcatatct tgtttcgtaa atgtaatttc
      721 attaccttca aaatccacaa caagtacatc tttattaagg gcattttctt tcgcccaaaa
      781 aataccaaca ataactccta tgtcaccatt gaatatgtta tcatttggcc tattaacaag
      841 ttgtaatact ttgtcacctt ttctaaagac tacatcacca aactcaattt ctcgtgtgtc
      901 tttcttttta gggtttaaaa tatcttgtaa aacttgattt aaacgtttaa taccggcatt
      961 tcctttatac attggtgcaa gcacttgaat atcagccata gtataccctt tattaacagc
     1021 actagtaact accttctcaa caactgttgg tatttggttt gcctgacagt taataaaact
     1081 tctatcatga aaacgctgtg taatatcaat tttctgaccc aatttcattc gatgtgctaa
     1141 ttctataatg cttgaaccat cttgttgacg atatacttca gtcagattaa ctcgtggtat
     1201 agctttcgat tcaattaaat ctttaaatac ttgaccagga cctacagaag gcaattggtc
     1261 ctcatcacct acaaatatca attgtgcatc taaaggaact gcacttaaaa attggtgaaa
     1321 caaccaagta tctaccatag acatctcatc aatgattatg agtcgtgcgt ttatttcatt
     1381 ttctaatata tcctctggct ttgtgtcttg attccaacct attaaacgat gaatcgtcat
     1441 tgcttctaat ccagttgact cttgtagtct cttagacgct cttcctgttg gcgctgctaa
     1501 tacaactgga taatcatcat tgacataatc atcataatct aatgataagc catgaatctc
     1561 agcatataat tctacaatac ctttaattac tgtcgtcttt cctgttcccg gtccaccggt
     1621 taatagcatc accttagaat tgatagccgt ttgcaaagct tctttttgtg aagctgcata
     1681 gttcacttga ttcgcatctt ctatttcacc aatatgcatt tgtaaatctg actgttcaat
     1741 ttccgtaagt ttatttgtat gcgtctttat tctgaataag ttttgaacac ttttgatttc
     1801 agaataatac aaacttggaa ttgcaacttg ttcattgtca ataattagtc gtttttcctc
     1861 atttaagtat tgcaacattt cgtctaattt ttcaggttcg atgacctctt catcttgata
     1921 atttaataca tcaaccgtta aatctataac aacattgata ggcaaatatg tatgtccctg
     1981 tttaatacat tcttcttcta acgtatagag caacgcagct tttaatcgtt cattatcgtt
     2041 ataagcgata ccaatatttc tagcaagttg atctgcttta ttaaaaccaa tacctttaat
     2101 atcataaatc aattgatatg gatttcgatc taaaatagtc agtgtatcgc cgagataaaa
     2161 ctgataaatt gccattgaaa gtttaggacc aaaccctaaa tcatgtaaac gaatcattat
     2221 tttttcagat tcttgatttg ctgaaatttg ttctgcaatt tgtttctgtt tctttttaga
     2281 taatcccgaa actttttcta gcactgaatg gtcatctaat atatcattta tcgcattgtc
     2341 acctaatgta ttaacaatat tttgagctgt ctttttacct acacctttaa acaaatcact
     2401 agataaataa cttataattg cttctttcgt ttgtggcatt tctttttcaa aagtctctgc
     2461 ttttaattgt ttaccataac gtggatgatc aacaacttgc cctttaaatg tgtagacatc
     2521 gccttcaaca atattcggaa gaaaccctac aacagttggc attgtatcaa agtcttcatt
     2581 tgtttcaata gtatctactt taagcactgt ataaaaatta tcactgtttt gaaacaatat
     2641 cgcttcaaca gtacctttga tcattgaata atcaaatagt gtagggtctg acatgttact
     2701 cctcctcttt cattttagtg aatgttttca gcgcatgctg acttaataag tgtttagggt
     2761 cgatagtcac agcttcttta aaatgagtta ttgcttcatc aatatcttca tttttcataa
     2821 ataacgctaa gcccaaattg tatcttgcat caacatgatt tttatcaatc gttaatacat
     2881 gtttaagttg agttatggct tcattaaaca tttctaattg acataataca agaccatatt
     2941 gaaattgaac ttctgcatct ttgtctttat ctagttccgc agcagtcatt aaatacggca
     3001 atgccagctt aaatgattct aactgattaa acgccatacc gatcatataa ttacaatcaa
     3061 cttgttcaat ctctgtttgt aatgcttgtt gatataattt aatagcttct tgataacgtt
     3121 gctgattata atatacattt gctagattat aaaatacgac gccattcttc ggatctattg
     3181 ttaaagcttt ttggaaaaaa cgctctgcct tttcaatctc attcgcatca gcaagtacga
     3241 taccagcatt aatataattt tcaataattg taggatcttc ttcgatattt ccgaacaatg
     3301 cttgtaacgc ttcttctatt tttccatttt gtatgtattg ataaattgtt tgttgatcta
     3361 tcatttacga acctcatttc tcatcaatta taacatcttg ataaattgta tgtctcgaat
     3421 cacttaacaa cgaataaaat ataatctaat atcattttca ttcatgaaaa agcgggaatg
     3481 gaatagaaat gctaaagaac cattaacggt ttattatgta atggttcttc cacattagcc
     3541 accactatta tgtacttaaa aataggaata cataattaga ttcatgcata gggagtggga
     3601 cagaaatgat attttaacaa aattaaattc gttatcccca actgg
//
LOCUS       DAOBBH010000210         3600 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.210, whole genome shotgun sequence.
ACCESSION   DAOBBH010000210 DAOBBH010000000
VERSION     DAOBBH010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.210"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(36..896)
                     /locus_tag="P6L01_002367"
     CDS             complement(36..896)
                     /locus_tag="P6L01_002367"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002468309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDP3031409.1"
                     /translation="MPLVSMKEMLIDAKENGYAVGQYNINNLEFTQAILEASQEENAP
                     VILGVSEGAARYMSGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFT
                     SVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGGQEDDVVADGIIYADPKE
                     CQELVEKTGIDALAPALGSVHGPYKGEPKLGFKEMEEIGLSTGLPLVLHGGTGIPTKD
                     IQKAIPFGTAKINVNTENQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKI
                     KEFGTSNRAK"
     gene            1114..1635
                     /locus_tag="P6L01_002368"
     CDS             1114..1635
                     /locus_tag="P6L01_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 domain-containing protein"
                     /protein_id="HDP3031410.1"
                     /translation="MSKILNTQLIGIFNRLEKQSLEIQMAAQCLIQAIGGEGYVYVKG
                     YDDLQFFESFILHSDERLKSSRKLDAIKDFKEIDSTDRVLLFAPFYNDQVALDIQKLI
                     DLDIDVVLISNKPKTDDFPDHLVHFIDLSTPRPIVYTEDYDKIVQPHAIALNYVYYDI
                     YTQMIEMTRDLEL"
     gene            complement(1744..3354)
                     /locus_tag="P6L01_002369"
     CDS             complement(1744..3354)
                     /locus_tag="P6L01_002369"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDP3031411.1"
                     /translation="MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKE
                     RRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQ
                     IRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMT
                     QDLKDKIALFCDINKESVIECRDADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLD
                     EWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSE
                     VTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEF
                     SRNVLGLEGAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYPCSIKEGTLAQDV
                     YGKAEIEERHRHRYEFNNDYREQLEANGMVISGTSPDGRLVEMVEIPTNDFFIACQFH
                     PEFLSRPNRPHPIFKSFIEASLKYQQNK"
BASE COUNT         1135 a          663 c          526 g         1276 t
ORIGIN      
        1 cctacgttat taataactta aaaactaaat attaattatt tagcgcggtt tgaagtaccg
       61 aactctttaa ttttaccttt aactgtttct ttgatggctt cacgtgcagg tcctaagtat
      121 ttacgaggat cgtaaacttc tttgtcgtta tttaaaacgt cacgaactgc ttttgctgaa
      181 gcgatttggt tttcagtgtt tacgttaatt ttagctgtac caaatggaat tgctttttgg
      241 atatctttag tcgggatacc agtaccaccg tgtaatacta atggtaaacc tgtagataaa
      301 ccgatttctt ccatttcttt aaatcctaat tttggttcac ctttgtatgg accatgaact
      361 gaacctaatg ctggcgctaa tgcatcaata ccagtttttt caactagttc ttgacattct
      421 ttaggatcag cataaatgat gccgtctgct acaacatcat cttcttgtcc accaacagta
      481 cctaattcag cttctacaga aacacctttt tcatgagcgt attcaacaac ttttttagtt
      541 gttgctacgt tttcttcgaa tgggctgtgt gaagcatcga tcattactga tgtgaaacca
      601 gcatcgatag cttctttaca tttttcaaag cttgaaccat ggtctaaatg gattgctaca
      661 ggaatagtga tgtttaagtc atgcattaac ccttcaacca ttttaacaat tgtgtagaaa
      721 ccgctcatgt aacgagcagc accttcagaa acacctaaaa ttacaggtgc attttcttct
      781 tgtgacgctt ctaaaattgc ttgagtgaat tctaggttat taatattgta ttgacctacc
      841 gcataaccat tttcttttgc atcaattaac atttctttca ttgaaactaa aggcatgaaa
      901 gttcctcctt gagtgcttac gcacgtattt ttcaaagtaa ttatatcaaa aaaatagcgt
      961 caatgcattg aaatactgca gattaaatgt gtattttttg aaaaatgcga tagttatact
     1021 aaatgcaaac gtatacattt ttatacaatt taagccgttg cataatatgt agtcattaat
     1081 tacaatattt tataagtaga ttggaggata aagatgtcaa aaatcttaaa cacacaacta
     1141 ataggtatct ttaataggct agaaaaacaa tctttagaaa ttcaaatggc cgcccaatgt
     1201 ttaattcaag caattggtgg cgaaggttat gtttatgtaa aaggttatga tgatttacag
     1261 tttttcgaaa gtttcattct acatagtgat gagcgtttaa aatctagccg aaaacttgac
     1321 gctattaaag actttaaaga aattgactca actgatcgtg tattattatt cgcaccattt
     1381 tataatgatc aagttgcatt agatattcaa aaacttatcg acttagatat cgatgtggtc
     1441 ttaataagca acaaacctaa aacagatgat ttccctgatc atctcgtaca ttttatcgat
     1501 ttatctacac caagacctat agtctatact gaagattatg ataagattgt ccagccacat
     1561 gcgatagcat tgaattatgt atactatgac atttatacac aaatgattga aatgacaaga
     1621 gacttagaat tataaaagtc actattgtta tttcatacct tgtcactaac ataatattaa
     1681 aattatacga aaaaccggta ctttcaatgt ttaacaatga aagtaccggt tttattagca
     1741 aatttattta ttttgttgat attttaatga agcttcaata aatgatttaa aaatcgggtg
     1801 cggacgattt ggtctagata agaattctgg gtggaattga caagcaataa agaaatcatt
     1861 tgtcggaatc tctaccattt ctactaaacg tccatctgga cttgtaccag aaattaccat
     1921 accatttgct tctaattgtt ctctatagtc attattaaat tcataacgat gacgatgtct
     1981 ttcttcaatt tccgctttac cataaacatc ttgtgccaat gtgccttctt taatagaaca
     2041 tggatataag cctaagcgta atgtaccacc taaatcttcg atatcttttt gttctggtaa
     2101 taaatctata attgggtatg gtgttgctgg gtctaattca gctgaatgtg cgccttcaag
     2161 tcctaatacg tttcttgaaa attcaactgt tgcaagttgc attcctaaac aaataccaaa
     2221 gaatggtaca ttgttttctc tagcatactt aattgcacta attttacctt cacttgcacg
     2281 gaaaccaaat ccacctggta ctaaaatacc gtcgacatct gcaaggtatt cggctgcatt
     2341 ttcatctgtt acttcacttg aatcaatcca tctaatgtca atatctttgg caaaaggata
     2401 tccagcatgt ttcaatgatt caacaactga taaatatgca tcttgtaagc taacatattt
     2461 acctactaaa ccaattgtaa ttttaccatc taaattatta acgatatcta acaactgttt
     2521 ccattcatca agctgtgttt catatttcgc gtttaattgt aaacgtttaa taacgatatc
     2581 atccatattt tgttggctta attgtaatgg aatttcgtat aaagagtctg catcacgaca
     2641 ttcaataaca ctttctttat taatgtcaca gaataatgca attttatctt ttaaatcttg
     2701 tgtcatttca tattcagttc ttacaacgat taagtctggt tgaataccta agcctcgtaa
     2761 ttctttaaca ctatgttgtg ttggcttcgt tttcatttct ccagcagctt taatataagg
     2821 cagtaatgta cagtgaacat acataacatt ttctctacct aaatcgctac gaatttgacg
     2881 aatcgcttca ataaacggta atgactcaat atcacctgtt gtaccgccaa tttcagtgat
     2941 aacaacgtct gcattcgtac tttcccctgc aagtaataaa cgttctttaa tttcatttgt
     3001 aatatgcgga ataacttgaa ctgttccgcc taagtaatca ccacgacgtt cttttttcaa
     3061 tacgtgtgaa tacactttac cggctgtcac atttgaaaac ttgtttaaat taatatcaat
     3121 aaatctttcg taatgtccta agtctaggtc agtttctgca ccatcatccg ttacgaatac
     3181 ttcaccatgt tgataaggac tcattgtacc tgggtcaaca tttaagtatg gatcgaattt
     3241 ttgaattgtt acatttagac ctctatcttt taataatcta cctagagaag atgctgtaat
     3301 acccttccct aatgatgaaa ctacgccacc tgttacaaaa ataaattttg tcatgtcttg
     3361 acctcctaat tattaaaggg aatgcttcct catcaattgt aaaaattacg taaaacataa
     3421 cattaaagca agctcattca agttgtttat ttgtcgaatt cgacaaaatt aaattctgtt
     3481 tacttaccgt ttccatactg ctatgtcact atgtatatca tttacaactt tgatttttgc
     3541 gttatcattt cttgatttac tcaattaaat aaaaaaacgc tccctctcac aaattctgta
//
LOCUS       DAOBBH010000211         3505 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.211, whole genome shotgun sequence.
ACCESSION   DAOBBH010000211 DAOBBH010000000
VERSION     DAOBBH010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.211"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            59..1528
                     /locus_tag="P6L01_002370"
     CDS             59..1528
                     /locus_tag="P6L01_002370"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDP3031412.1"
                     /translation="MYQLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMP
                     FNSGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQE
                     GELCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGT
                     RRAQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKK
                     YAERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKEARRM
                     LDEAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVA
                     IENEEGSYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPL
                     KLFHPVHTYKMKFIKSFEAIDLHHNIYENGKLVYQMPTEDESREYLAQGLQSIWDENK
                     RFLNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE"
     gene            1521..2342
                     /gene="nadE"
                     /locus_tag="P6L01_002371"
     CDS             1521..2342
                     /gene="nadE"
                     /locus_tag="P6L01_002371"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDP3031413.1"
                     /translation="MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSL
                     VLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVKLPYGVQKDADEVDQALRFIEP
                     DEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
                     HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKP
                     QLPDEDALGVTYEAIDNYLEGKPVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS"
     gene            2613..3215
                     /locus_tag="P6L01_002372"
     CDS             2613..3215
                     /locus_tag="P6L01_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDP3031414.1"
                     /translation="MSFVTENPWLMVLTIFIINVCYVTFLTMRTILTLKGYRYIAASV
                     SFLEVLVYIVGLGLVMSNLDHIQNIIAYAFGFSIGIIVGMKIEEKLALGYTVVNVTSA
                     EYELDLPNELRNLGYGVTHYAAFGRDGSRMVMQILTPRKYERKLMDTIKNLDPKAFII
                     AYEPRNIHGGFWTKGIRRRKLKDYEPEELESVVEHEIQSK"
     gene            3196..3369
                     /locus_tag="P6L01_002373"
     CDS             3196..3369
                     /locus_tag="P6L01_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NETI motif-containing protein"
                     /protein_id="HDP3031415.1"
                     /translation="MKFKVNENETIADCLSRMKMQGYMPVKRIEKPVFQEQKDGTVEV
                     SHQEIVFVGKKIQ"
BASE COUNT         1280 a          455 c          689 g         1081 t
ORIGIN      
        1 aaaaaaatga attatgataa aatgtaagaa aatatttagg tcgattggag agatacaagt
       61 gtaccaatta gaagacgaca gtttaatgtt acataatgac ttatatcaaa taaatatggc
      121 tgaaagttat tggaatgata atattcatga aaaaatggct gtatttgatt tgtattttag
      181 aaaaatgcca tttaatagtg gctatgctgt ttttaatggt ttaaaacgcg tcattgattt
      241 tatagaacat tttggatttt cagaatctga tttggaatat ttaaagtcta ttggctacaa
      301 ggatgatttc ttatcatatt taaaagattt aaaattcaca ggaagcattc gttcgatgca
      361 agaaggcgaa ttatgctttg gtaacgaacc attgttacgc gtagaagcac cattgattca
      421 agcgcaatta atagaaacaa ttttattaaa cattgtaaat ttccatacac taattacaac
      481 aaaggctagt agaattcgtc aaattgcatc aaatgataaa ttaatggagt ttggtacacg
      541 tcgtgcgcaa gaaattgatg cagcattgtg gggcgctaga gctgcttaca ttgggggctt
      601 tgattctaca agtaatgtta gggcaggtaa attatttggt atacctgtgt ctggtacaca
      661 tgcacacgca tttgtccaaa cttatggaga tgaatatgtt gccttcaaaa aatatgctga
      721 aagacataaa aattgtgtgt tcctagtaga tacattccat actttaaaat ctggcgtgcc
      781 aaatgcaata aaagttgcaa aagaattagg tgacaaaatt aactttgtag gtattcgatt
      841 ggattctgga gatatcgctt atttatctaa agaggcaaga cgtatgcttg atgaagcagg
      901 atttactgaa actaaaatta tcgcgtctaa tgatttggat gaagaaacga ttacgagttt
      961 gaaagcacaa ggtgcaaaag tagattcttg gggcgttggt acaaagctga ttacaggata
     1021 cgatcaacca gcattaggtg cagtatataa acttgtagct attgaaaatg aagagggttc
     1081 atatagtgat cgtattaaat tatcaaataa cgctgaaaag gttacgacgc caggtaagaa
     1141 aaatgtatat cgcattataa acaagaaaac aggtaaggca gaaggcgatt atattacttt
     1201 ggaaaatgaa aatccatacg atgaacaacc tttaaaatta ttccatccag tgcatactta
     1261 taaaatgaaa tttataaaat ctttcgaagc cattgatttg catcataata tttatgaaaa
     1321 tggtaaatta gtatatcaaa tgccaacaga agatgaatca cgtgaatatt tagcacaagg
     1381 attacaatct atttgggatg aaaataagcg tttccttaat ccacaagaat atccagtcga
     1441 tttaagcaag gcatgttggg ataataaaca taaacgtatt tttgaagttg cggaacacgt
     1501 taaggagatg gaagaagata atgagtaaat tacaagacgt tattgtacaa gaaatgaaag
     1561 tgaaaaagcg tatcgatagt gctgaagaaa ttatggaatt aaagcaattt ataaaaaatt
     1621 atgtacaatc acattcattt ataaaatctt tagtgttagg tatttcagga ggacaagatt
     1681 ctacattagt tggaaaacta gtacaaatgt ctgttaacga attacgtgaa gaaggcattg
     1741 attgtacgtt tattgcagtt aaattacctt atggagttca aaaagatgct gatgaagttg
     1801 accaagcttt gcgattcatt gaaccagatg aaatagtaac agtcaatatt aagcctgcag
     1861 ttgatcaaag tgtgcaatca ttaaaagaag ctggtattgt tcttacagat ttccaaaaag
     1921 gaaatgaaaa agcgcgtgaa cgtatgaaag tacaattttc aattgcttca aaccgacaag
     1981 gtattgtagt aggaacagat cattcagctg aaaatataac tgggttttat acgaagtacg
     2041 gtgatggtgc tgcagatatc gcacctatat ttggtttgaa taaacgacaa ggtcgtcaat
     2101 tattagcgta tcttggtgcg ccaaaggaat tatatgaaaa aacgccaact gctgatttag
     2161 aagatgataa accacagctt ccagatgaag atgcattagg tgtaacttat gaggcgattg
     2221 ataattattt agaaggtaag ccagttacgc cagaagaaca aaaagtaatt gaaaatcatt
     2281 atatacgaaa tgcacacaaa cgtgaacttg catatacaag atacacgtgg ccaaaatcct
     2341 aatttaattt tttcttctaa cgtgtgactt aaattaaata tgagttagaa ttaataacat
     2401 taaaccacat tcagctagac tacttcagtg tataaattga aagtgtatga actaaagtaa
     2461 gtatgttcat ttgagaataa atttttattt atgacaaatt cgctatttat ttatgagagt
     2521 tttcgtacta tattatatta atatgcattc attaaggtta ggttgaagca gtttggtatt
     2581 taaagtgtaa ttgaaagaga gtggggcgcc ttatgtcatt cgtaacagaa aatccatggt
     2641 taatggtact aactatattt atcattaacg tttgttatgt aacgttttta acgatgcgaa
     2701 caattttaac gttgaaaggt tatcgttata ttgctgcatc agttagtttt ttagaagtat
     2761 tagtttatat cgttggttta ggtttggtta tgtctaattt agaccatatt caaaatatta
     2821 ttgcctacgc atttggtttt tcaataggta tcattgttgg tatgaaaata gaagaaaaac
     2881 tggcattagg ttatacagtt gtaaatgtaa cttcagcaga atatgagtta gatttaccga
     2941 atgaacttcg aaatttagga tatggcgtta cgcactatgc tgcgtttggt agagatggta
     3001 gtcgtatggt gatgcaaatt ttaacaccaa gaaaatatga acgtaaattg atggatacga
     3061 taaaaaattt agatccgaaa gcatttatca ttgcgtatga acctcgaaac atacatggtg
     3121 gattctggac taaaggcatt cgtcgtagaa agcttaaaga ttatgaacca gaagaactgg
     3181 aaagtgtagt agaacatgaa attcaaagta aatgagaatg aaacaattgc tgattgtttg
     3241 tcacgaatga aaatgcaagg gtatatgccg gtaaaacgta ttgaaaaacc cgtgtttcaa
     3301 gagcaaaaag atggcacggt tgaagtatca catcaagaaa tcgtttttgt aggtaagaaa
     3361 atccaataac ataatccaat ttaaataaag actatttgaa gaggaaaggc tattcaaagt
     3421 ttgagtaatt ttactttgaa tagcctattt gtttatacat gcaagatgct cgatccatat
     3481 tgtatgagaa acccccagca agcta
//
LOCUS       DAOBBH010000212         3493 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.212, whole genome shotgun sequence.
ACCESSION   DAOBBH010000212 DAOBBH010000000
VERSION     DAOBBH010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.212"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(196..303)
                     /locus_tag="P6L01_002374"
     CDS             complement(196..303)
                     /locus_tag="P6L01_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031416.1"
                     /translation="MVLLAFVTTIHDTDDGRFFNEHKHANNKLLKHSLI"
     gene            complement(340..663)
                     /gene="isdG"
                     /locus_tag="P6L01_002375"
     CDS             complement(340..663)
                     /gene="isdG"
                     /locus_tag="P6L01_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdG"
                     /protein_id="HDP3031417.1"
                     /translation="MKFMAENRLTLTKGTAKDIIERFYTRHGIETLEGFDGMFVTQTL
                     EQEDFDEVKILTVWKSKQAFTDWLKSDVFKAAHKHVRSKNEDESSPIINNKVITYDIG
                     YSYMK"
     gene            complement(682..1416)
                     /gene="srtB"
                     /locus_tag="P6L01_002376"
     CDS             complement(682..1416)
                     /gene="srtB"
                     /locus_tag="P6L01_002376"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HDP3031418.1"
                     /translation="MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQ
                     KFQMLMSKHQEHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFER
                     EHRRKGSIFMDFRNELKNLNHNTILYGHHVGDNTMFDVLEDYLKQSFYEKHKIIEFDN
                     KYGKYQLQVFSAYKTTTKDNYIRTDFENDQDYQQFLDETKRKSVINSDVNVTVKDKIM
                     TLSTCEDAYSETTKRIVVVAKIIKVS"
     gene            complement(1478..2443)
                     /gene="isdF"
                     /locus_tag="P6L01_002377"
     CDS             complement(1478..2443)
                     /gene="isdF"
                     /locus_tag="P6L01_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter permease protein IsdF"
                     /protein_id="HDP3031419.1"
                     /translation="MIKNKKKLLFLCLLVILIATAYISFVTGTIKLSFNDLFTKFTTG
                     SNEAVDSIIDLRLPRILIALMVGAMLAVSGALLQAALQNPLAEANIIGVSSGALIMRA
                     LCMLFIPQLYFYLPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLE
                     ILTQNPLMKIPQGLTMKIWSDVYILAVSALLGLILTLLLSPKLNLLNLDDIQARSIGF
                     NIDRYRWLTGLLAVFLASATVAIVGQLAFLGIIVPHVVRKLVGGNYRVLIPFSTVIGA
                     WLLLVADLLGRVIQPPLEIPANAILMIVGGPMLIYLICQSQRNRI"
     gene            complement(2456..3334)
                     /gene="isdE"
                     /locus_tag="P6L01_002378"
     CDS             complement(2456..3334)
                     /gene="isdE"
                     /locus_tag="P6L01_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HDP3031420.1"
                     /translation="MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALT
                     MTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDE
                     MQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKA
                     AKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLN
                     INPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDA
                     DKAMTQLYDLFYKDKK"
     gene            complement(3331..>3493)
                     /locus_tag="P6L01_002379"
     CDS             complement(3331..>3493)
                     /locus_tag="P6L01_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HDP3031421.1"
                     /translation="DDSKNSSQLSSDIKELDEPNHKKQYMLFAAGIVLATILLISAHL
                     YSRKRGNQV"
BASE COUNT         1134 a          615 c          437 g         1307 t
ORIGIN      
        1 taataagatg aaaacatttg attgtatagt taatattaat taatcgcttt tatcactcat
       61 aatatttcaa attgcataaa tttcttttat cgatactact actataaatc atacgcccca
      121 aaatatcatt attaattctt ttcttcttca aaataaatca aaatgatata attgatgatt
      181 attttcaaag cacattcaaa tcaaactatg ttttagcaat ttgttgttag catgtttgtg
      241 ttcattaaaa aaacgaccat catcggtatc atgtatggtc gttacaaaag ctaacaatac
      301 caattgtcat aacaagtact gcaacctctt taaattcaat tatttcatgt aactatagcc
      361 tatatcatat gtaattactt tgttattgat aatcgggcta ctttcatctt catttttact
      421 tctaacatgt ttatgcgctg ctttaaagac atcagatttt aaccaatccg taaaagcttg
      481 ctttgatttc caaactgtta aaattttcac ttcatcaaaa tcttcttgtt ctaaagtttg
      541 tgtaacaaac atgccatcaa agccttctaa tgtttcaatc ccatgtctcg tgtaaaatcg
      601 ttctataata tcttttgctg ttccttttgt taacgtcagc ctattttctg ccataaattt
      661 cataattatc ctcttttctg tttaacttac cttaattatt tttgcgacaa caacaattct
      721 tttcgttgtt tcactatatg catcttcgca cgttgataaa gtcattattt tatcttttac
      781 cgttacatta acatctgaat taattacaga tttacgtttt gtctcatcta aaaattgttg
      841 ataatcttga tcattttcaa aatctgtacg tatgtaatta tctttagtag tagttttata
      901 tgcactaaat acttgcaatt gatatttacc atatttattg tcaaattcaa ttatcttgtg
      961 tttttcataa aacgattgct ttaaataatc ttctaacaca tcaaacatcg tattatcacc
     1021 gacatggtgc ccgtataaaa tagtattatg atttaaattc ttcaattcat ttctaaaatc
     1081 cataaaaata ctacctttac gtcgatgttc tcgctcaaaa tctaaattta aataatcgtg
     1141 atttgtctta ccttgtagta ctggataatt taatgatgtt cctgataatt ttatccatcc
     1201 aacaatgtct ttatttattt tttcaagtga ttcaaattgt ggtctcacat gttcttgatg
     1261 tttgctcatc agcatttgaa atttttgttg taatttctca taatttgcgc gttcttgctt
     1321 gtcttcaata tatgtttgaa caattttgta accaaaaatg ataataatta caaccaataa
     1381 aatttgtaca atagttaaaa atcgcttcat tctcataaaa atcctctttt attaacgacg
     1441 tttcttcagt catcactaaa ccagttgttg taccgtttta gattcgattt cgttgacttt
     1501 gacaaattaa gtaaattagc attggaccac cgacaatcat taaaatagca ttggctggaa
     1561 tttctaaagg aggctgtatc actcgtccta ataaatcagc cactaacaat agccatgcac
     1621 caataactgt agaaaacgga ataagtactc tgtaattgcc cccaactagc tttctaacca
     1681 catgtggcac aataatacct aaaaaggcta gttgtccaac aatcgcaaca gttgcacttg
     1741 ctaaaaatac tgctaataaa cctgttaacc atctgtaacg atcaatatta aaaccgatac
     1801 ttcgcgcttg tatgtcgtct aaatttagta aattcaattt aggggacaat agtaatgtta
     1861 atattaatcc caataatgct gatactgcta atatgtatac gtcgctccat attttcattg
     1921 ttaagccttg aggaattttc attaaagggt tttgagttaa aatttctaaa acaccattta
     1981 ataatacgaa taacgcaaca cctactaata tcatacttac agcattgaat ctaaatttag
     2041 aatgcaacaa tataattatt aaaaatggta ttaaacctcc aataaaactt aataatggta
     2101 agtaaaagta caattgtgga ataaacaaca tacaaagtgc tctcattata agtgcacctg
     2161 aggaaacgcc aatgatattc gcctctgcca aaggattttg tagtgctgct tgtaataatg
     2221 ctccagaaac tgctaacatt gcgccaacca tcaatgcaat taatatacgt ggcaatcgca
     2281 aatcaatgat tgaatccact gcttcattgc taccagttgt aaattttgta aataggtcat
     2341 taaatgacaa tttaattgta ccggttacaa acgaaatata agcagttgcg attaaaatga
     2401 ctaacaaaca taaaaatagt agtttctttt tattttttat catcatatca actcactatt
     2461 ttttatcctt ataaaataaa tcatataatt gagtcattgc cttatcagca tcaacatttg
     2521 ctgtaatacc gaatggcact tcctctaagt cataaacatg attatttttc acagctttaa
     2581 aatgtttcca aatatcattt tgtttaaatt ctttttgaaa cattttctta acttcttcag
     2641 gcattccgtg tggtaatcgt aaaataatat ctggattgat attcaacaaa ttttcagtat
     2701 tagacgaaat atattgacga tctttcactt taataacatt ttctccacct gctattttaa
     2761 ctaaatcacc aatatatgat ttatcagttg ctactaaata gctacccggt acacccatta
     2821 atattaatac tttgggatgt ttcttttgtt tagctgcttt attttcaatt ttttgcttta
     2881 cagaatttaa atggtcattt aattcttttg cttgtgcttt acgattaaat tgatcaccta
     2941 attgtgtaat cgacttttgc atccctttta aactatcaaa atcataaaag tagcctttca
     3001 tatttaattg tttgtaaaat ggttgcattt catctttaat cgttgacaca ctcaaaacat
     3061 gtgttggttt taacttttta acagcttcaa cattcggctc cattggttga ccaatttccg
     3121 gtacatcttt ataacgatta ggcaatgtct tatatgacgt gggtttgccg acaattggta
     3181 aatccaattt gtccaatgtc attgtcaatg caacagttgt tggtacgatt ctgaattcgc
     3241 cggatttagt tgattcttga gaactggaag attgacagct ggttaagata acgacgctta
     3301 tcactaaaat ggttaaatac tttatgattc tcaaacttgg ttacctctct ttctgctgta
     3361 taaatgtgcc gaaataagta aaatagttgc taacacaatg ccagctgcaa ataacatata
     3421 ttgcttttta tgatttggtt catcaagttc tttaatatca ctagataatt gactactatt
     3481 cttgctatca tca
//
LOCUS       DAOBBH010000213         3415 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.213, whole genome shotgun sequence.
ACCESSION   DAOBBH010000213 DAOBBH010000000
VERSION     DAOBBH010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.213"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            74..340
                     /locus_tag="P6L01_002380"
     CDS             74..340
                     /locus_tag="P6L01_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031422.1"
                     /translation="MKGSKQILLIMGIISLIVLFIFTLFIMAQYAKHYEQKSDSSNAH
                     TLNSSSAIIEQHTISNLASLDLYAPVRNITSSRDIHPIYFMTKD"
     gene            484..792
                     /locus_tag="P6L01_002381"
     CDS             484..792
                     /locus_tag="P6L01_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031423.1"
                     /translation="MTVNIVFSIVFCISMVILGIYVAITKDFTLISYINQTTIADKHK
                     NQIAYIFTLCISLSAVFLMSSILCFEYDFIALSFLFLTIALLLIALFYVCFYKITKYP
                     "
     gene            complement(900..1133)
                     /locus_tag="P6L01_002382"
     CDS             complement(900..1133)
                     /locus_tag="P6L01_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031424.1"
                     /translation="MLRSLCKQNRILINAIKVGIEMKYKISLAYNLAIIIGSLIILCI
                     LISRGYDIYVILIPILTILASLINLICDIKKHK"
     gene            complement(1105..2306)
                     /locus_tag="P6L01_002383"
                     /pseudo
     CDS             complement(1105..2306)
                     /locus_tag="P6L01_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(2690..>3415)
                     /locus_tag="P6L01_002384"
     CDS             complement(2690..>3415)
                     /locus_tag="P6L01_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E domain-containing protein"
                     /protein_id="HDP3031425.1"
                     /translation="VNELTEFGGEKIPQGHKDIFDPNLPTDQTEKVPGKPGIKNPDTG
                     KVIEEPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTIT
                     TPITVNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKPKDPKGPENPEKPSRPTHPSG
                     PVNPNNPGLSKDRAKPNGPVHSMDKNDKVKKSKIAKESVANQEKKRAELPKTGLESTQ
                     KGLIFSSIIGIAGLMLLARRRKN"
BASE COUNT         1135 a          591 c          451 g         1238 t
ORIGIN      
        1 aaaaaggttt agaattacaa aaataactat tttaaattta gttacaaata tattttagaa
       61 aggatgtgaa ataatgaaag gctctaaaca aatacttttg attatgggca ttatatctct
      121 tattgtttta tttattttta cactattcat catggcacaa tatgcaaaac attatgaaca
      181 aaaatccgac agttccaacg cacatacttt aaactcatca tcagccataa ttgagcaaca
      241 tactatatcg aatttagcgt ctctagattt atacgctcct gttcgtaaca ttacttctag
      301 tcgtgatatt catcccattt atttcatgac aaaagattaa aacttccttt ttcataaccc
      361 cattcattca actaataaat taaaaattga taaaaacgta ctgtttttta gaaacataca
      421 tatataagat acgcattcat tactaaaaga cagtacatta tattctatca ggaaggtgca
      481 acaatgaccg tcaacatcgt atttagtatc gtattctgca taagtatggt tatattaggc
      541 atttatgtcg caataactaa agatttcaca ctaatttctt atataaatca aacgaccatt
      601 gcagataaac acaagaacca aattgcatat atatttacgc tatgtatcag tttgagtgca
      661 gtatttttaa tgtcttcaat tctatgcttt gaatatgatt ttattgcatt gtcattctta
      721 tttttaacga tagcattatt acttatcgca ttattctacg tttgctttta taaaatcaca
      781 aaatatccat aaatgtttgc gatagtgtgt tggttagatt tattgatgcg ctgtcattaa
      841 gtaattgttt gcgaaaaaaa tacaaccttg tacgaataca aagttgcatt aatatagaat
      901 catttatgct ttttaatatc acaaattaaa ttgattaaac tagctaaaat agtcaaaatt
      961 ggaatcaaga ttacatatat atcataacct ctagatatta gtatacataa tataattagc
     1021 gaccctatta taattgctaa attataagcc aaactaattt tatatttcat ttcaattccc
     1081 accttaatag cattgattaa aattctattt tgtttgcata gacttcttaa caaactttaa
     1141 cccagctaag ctaagcattg aaataattgc acctcctgat ataaatatat tacttaagct
     1201 tagtaaaggt ataattaaac ttatcagtcc taagataatg tatccgctgc gtaattcgat
     1261 gtagatgaga tactaaatac tttccccatc aaatgatttg gcgtttttat ttgaatggca
     1321 actgatcttg ttagtccctc tatagattgt ccaagtccca ctaatgttgc acctatatat
     1381 agtatcgcca cacttggaaa cacattaatt atcgataagc caatccccca aactaaaaca
     1441 ccaatactaa atttaaagat taatcgtttt tctgatagta aacccataat caatgacatt
     1501 aataaagatg ctatacctaa acatgatgta gctagtccat atacaccaac gccctctttt
     1561 aatatattgg aaataaacaa tggtaatgca acacgccaaa ggccagtatt aatcaatatg
     1621 caggcaaatt ggatgattat gataaatgga atttctttag attgtttcaa gaattcccaa
     1681 gtttcagaaa aatcttcttt tgagtgtcta tcaatcatgt tgttatttgt atattttaaa
     1741 agtgcattaa aaataaatcc taaaaatagc aatatactgc aaataaaaaa gacgccaaca
     1801 ttaccaacta gtattacaat gacaccaatt aaagcaggta aaataatatt tgagcctctt
     1861 tgcaaactat cgattaacgc attacttgtt gctaaatgct cctcatcaat aatttcagga
     1921 agaattgccc taaacgcagg atccgtatag cagttaataa tggtgatagc tgtagatatg
     1981 gttagaagcg tcagataatt taaatttgat gttattgcaa gtaaaggaat aattattata
     2041 atcaaactta gtataagatc agatagataa agtattttct ttttattatg tttatcagaa
     2101 tatgcgccac cgaaaatacc aaatataata aatggaagtg tttgactcat aaccatcatt
     2161 gataatttta aagatgattg gtttgtcaat tcaacagtaa accaaattat ttgtaacgaa
     2221 aacagcacaa agcaactccg acgtaagaaa ttaccaatca ataaatatgt aaagtttcta
     2281 tttttcaaaa cttctaagta caacatattt atcacctctc ataaaaataa ttgaatgcat
     2341 ccaccagcct tttttagacc ttcttctaaa ctctctttat ccaaagcgca attaattcta
     2401 ataaaattaa gtcagttaaa tatcaattat ttcgaaatat acatactact tgaaacacca
     2461 tacatacccc ccaaaatgac tactcagagg ttatattcta ctaattatga ttatattaaa
     2521 tatgaaaata ttatcaaaaa atcaaattta taacaaaaat acacccctta aagttaggtg
     2581 tttcaatcca acttttgggg tgtatatcat tctcatcata ttctaggttg tttttaacaa
     2641 actaaatata gtgaatgcaa atcaactatt atttaaatta tgaattattt taattctttc
     2701 ttctacgagc caataacatt aatccagcaa ttccaattat actactaaag atcaaacctt
     2761 tttgcgtgct ttctaaacct gtttttggta attctgctcg ttttttctct tgattagcta
     2821 ctgattcttt agcaatttta gattttttaa ctttatcatt tttatccatt gaatgaactg
     2881 ggccatttgg ttttgctctg tctttcgata atcctggatt gttaggattt actgggccac
     2941 ttggatgagt tggtctgctc ggcttctctg ggttttcagg tccttttgga tcttttggtt
     3001 tctctccacc gaactctaca atcttatcta ctggttgttt tgtgatctct tctgttggtt
     3061 gaccctcgcc aactttttca cctgttaatg ggttcactgt gattggtgtt gtgattgtct
     3121 tacttcctgg ttgtccttct tgtttcactc gctcttcacc aggttgtaat tttggattaa
     3181 actcacgttt tgtttcaaac ggtatctcta ctgtttttgt ttctggtgta cccgtttttg
     3241 gtccgtgttt aatcacatca tccactggct cttcgatcac ttttcctgtg tctggattct
     3301 tgattcctgg tttacctggt actttttccg tttgatctgt tggtaagttt ggatcaaaga
     3361 tatctttatg accttgcggt attttctcgc caccgaattc tgttaattca ttaac
//
LOCUS       DAOBBH010000214         3394 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.214, whole genome shotgun sequence.
ACCESSION   DAOBBH010000214 DAOBBH010000000
VERSION     DAOBBH010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.214"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..424)
                     /locus_tag="P6L01_002385"
     CDS             complement(<1..424)
                     /locus_tag="P6L01_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HDP3031426.1"
                     /translation="MGKLGYKNILIDFDDTIVDFYDAEEWAFHYMANVFNHKATKDDF
                     LTFKKINHQHWEAFQQNKLTKSEVLSERFVNYFKHHQMEVDGHRADVLFRNGLAEAKV
                     KYFDQTLETIVELSKRHDLYIVTNGVTETQKRRLNQTPL"
     gene            complement(548..682)
                     /locus_tag="P6L01_002386"
     CDS             complement(548..682)
                     /locus_tag="P6L01_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HDP3031427.1"
                     /translation="MTGLAEAIANTVQAAQQHDSVKLGTSIVDIVANGVGLLGKLFGF
                     "
     gene            complement(739..873)
                     /locus_tag="P6L01_002387"
     CDS             complement(739..873)
                     /locus_tag="P6L01_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HDP3031428.1"
                     /translation="MEGLFNAIKDTVTAAINNDGAKLGTSIVSIVENGVGLLGKLFGF
                     "
     gene            1237..1310
                     /locus_tag="P6L01_002388"
     tRNA            1237..1310
                     /locus_tag="P6L01_002388"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1271..1273,aa:Arg,seq:tct)
     gene            complement(1778..1966)
                     /locus_tag="P6L01_002389"
     CDS             complement(1778..1966)
                     /locus_tag="P6L01_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDP3031429.1"
                     /translation="MRNQIQKLLDSDLSSLHISKQTGVPQSTIHRMRKKERSLDNMSL
                     KNAELLYKFANSIFSNEN"
     gene            2215..3162
                     /locus_tag="P6L01_002390"
     CDS             2215..3162
                     /locus_tag="P6L01_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDP3031430.1"
                     /translation="MRLQKAPLVTSGLVLGLLGLGNLLKDLSLTLNAVCGIFAFLIWI
                     HLLCTMIKYFNNVKEQLNSPLVSSVFTTFFMSGFLGTTYLNTFFSNITFINSLITPIW
                     ILCLVGIMTHMIIFSIKYLKDFSLENVYPSWTVLFIGIAIAGLTAPVSGCFFIGQLTV
                     IYGFVATCIVLPIVFKRLKAFPLQTSIKPNTSTICAPFSLVAAAYVIAFPKANAFIVI
                     IFLILAQIFYFYIIIQLPKLLKEPFSPVFSAFTFPLVISATALKNSLPVLMFPDIWKG
                     LLFIEVLLATVIVLRVFIGYLHFFLKKENQDKFLRNASQ"
BASE COUNT         1070 a          602 c          454 g         1268 t
ORIGIN      
        1 gcaacggcgt ctgatttaac cttctctttt gcgtttcggt tacaccatta gtaacaatat
       61 ataaatcatg tcttttcgat aattcgacaa ttgtttctaa tgtttgatca aagtatttaa
      121 ctttagcttc tgctaatcca tttctaaata acacatctgc acgatgccca tcaacttcca
      181 tttgatgatg tttgaagtaa ttcacaaatc gttctgataa tacttcagac ttcgttaatt
      241 tattttgttg aaaagcttcc caatgttggt gattgatttt tttaaatgtt aaaaaatcat
      301 cctttgttgc tttatgatta aaaacattcg ccatatagtg aaacgcccat tcttctgcat
      361 cataaaaatc aacaattgta tcatcaaagt ctatcaaaat atttttatat cccaatttcc
      421 ccatctccta tattgtctat gtatctaaat cttaacagag gctcaaattt ctgcaaataa
      481 aataaactga gtgcataaca ttaaagtatg ctcacccagt ttattttaaa gaatattagt
      541 tattatatta gaatccaaat aatttaccta gtaaacccac accgttagca acgatgtcta
      601 cgatacttgt gcctaatttc acactatcat gttgttgtgc agcttgcaca gtatttgcga
      661 ttgcttctgc tagtccagtc atttaaatct ctccctcacc tttgaaataa tactgattac
      721 ttacataaca tattgaaatt agaatccgaa taatttacct aataaaccta cgccattttc
      781 aacgatgctc acaatgcttg tgcctaattt tgcgccatca ttattaattg ctgcagttac
      841 ggtatcttta attgcgttaa ataaaccttc cattgaaaac actccttaaa atttaaattt
      901 gaagataaca aaaacgtgcg tagcttttaa atcaccgaaa tgttattcgc ttaacgtttt
      961 gttgttgtta ttttaaaata aatttgatgc aattagtttg tttatccgca caacatctta
     1021 taatgtactt aactgtattt taaagagaaa agaaatacag ttaggcattc aaaactgtat
     1081 ttaacacaat taagttgcct gaattcgcat ttaagtctta ttgaaccttt ttagataaat
     1141 agctctataa tagtgaaaaa tataaacatt ttttatttac aaggtattgc taatttaagt
     1201 tcatttagat ataataattc ttgtgttgtt aaacgtgtcc tggtagctca gctggataga
     1261 gcaatggcct tctaagccat cggtcggggg ttcgaatccc tcccaggacg tttataggta
     1321 tttttatacg cattaccaaa caaaagagtt ccgtgattac ggggctcttt ttgttttgaa
     1381 tttcagtaat atagtatgat gcgtcaccaa aacgtccccc gcataagccc cgaaaataca
     1441 gtaattaaaa caagcatgct tattcgtcat agaatttttt gacacacaat tgacacgcgt
     1501 ctgacacttg tttatacatt tttaattaag taattttgtg ctcaaatttc atctatactg
     1561 cacctgaact acaccaacac tacaccaaga tttttaacac tcaccatttg catgcgtaga
     1621 gatttttatt attatattat tcctatagat tttgatacta ttcaaaattt tagggacttt
     1681 tcaggggccc gaaatcctat aattaaatac atttaaaaaa ataaccacgt ccatcgagac
     1741 gtggtttttt aattatttaa ctcactaatt attcttctta attttcattg ctaaatatac
     1801 tattggcaaa tttataaagt agttcagcgt ttttcaatga catattgtct aatgatcttt
     1861 ctttttttct cattctgtgt attgtgcttt gtggaactcc tgtttgtttc gatatatgta
     1921 aactgctcaa atcactgtct aatagttttt gaatttgatt tctcattttg tacaccccct
     1981 tgtaaatctt caatcatcat ttaaattaaa taattacttt tccacatatt ccaatattta
     2041 ggttgcaaag catacctcaa atatcattaa ttttgagatt taaatgtcaa ttatgtgtct
     2101 atcaatccaa tatacatact ctaataacgt aatagtacac actcttctta ttaattggcc
     2161 atagctatca tgatataatt agtgaagaaa atcacataag aaaggttgta aatcatgaga
     2221 cttcaaaaag cacctctagt aacgtcagga ctagtcttag gattattagg cctgggtaat
     2281 ctattaaaag acttatctct tactttaaac gctgtttgcg gaatctttgc tttcttgatt
     2341 tggattcacc ttttatgtac tatgatcaaa tattttaata atgtgaaaga acaattaaac
     2401 agtcctctag tttcatcagt gttcacaaca tttttcatgt ctggcttttt aggtactact
     2461 tatttaaata cattttttag taacataact tttatcaata gcttaataac gcctatttgg
     2521 attttatgcc ttgtgggaat tatgacgcat atgattattt tttcaataaa atatttaaaa
     2581 gatttttcac ttgaaaatgt ttatccttcg tggactgtac tttttattgg tattgctatc
     2641 gcaggattga cggcacccgt tagcggatgt tttttcatag gtcaattaac agtaatatat
     2701 ggctttgtag ctacttgtat tgtcttacct attgttttca agcgattaaa agcatttcca
     2761 ttgcagacgt caatcaaacc gaacacatcg acaatttgtg caccattttc tttagtcgct
     2821 gcagcatatg ttatagcctt tcctaaggcg aatgctttta tcgtaattat atttttaata
     2881 ttagctcaaa tattttattt ttatatcatt atacaattgc ctaaattact aaaagaacct
     2941 ttttcgcccg tattttcagc tttcacattc cctttagtaa tctcagcaac tgctttaaag
     3001 aacagtttgc ctgtacttat gtttccagat atttggaaag gtcttttgtt tatcgaagtg
     3061 ttattagcca ctgtaatagt acttagagtc tttataggat atcttcactt ctttttaaaa
     3121 aaggaaaatc aagataaatt tcttcgtaat gcgtctcagt aacactatta ccaagaatta
     3181 acacgcatat ttaataacta aaaaagaggc aggtaatctt gcctcttttt agtgtatatc
     3241 aatgcctctt tgatttaata tgatcttatc aatgatacct tctgactaaa gctgtagtaa
     3301 ctatactaga acgtctagtt cataaacacg tttcactcta acctttattt aaataatata
     3361 taagcagcaa acgccaaggc taatatacca ataa
//
LOCUS       DAOBBH010000215         3377 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.215, whole genome shotgun sequence.
ACCESSION   DAOBBH010000215 DAOBBH010000000
VERSION     DAOBBH010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.215"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            326..862
                     /locus_tag="P6L01_002391"
     CDS             326..862
                     /locus_tag="P6L01_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDP3031431.1"
                     /translation="MITVLFGGSRPNGNTAQLTKFALQDLEYQWIDVTQHQFKPIRDV
                     RHTAETITSYDDDYLPILDKILASDTIIFASPVYWYSISAPLKAFIEHWSETLQDKRY
                     PNFKAQMAEKDFRVILVGGDCPKIKAKPAITQMKYSLDFLGATLNGYIIGTAEKPGDI
                     MKDNYALARATEWNSILQ"
     gene            1030..1506
                     /locus_tag="P6L01_002392"
     CDS             1030..1506
                     /locus_tag="P6L01_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDP3031432.1"
                     /translation="MFKVRQATEKDVVQIRDVATKAWFNTYLNIYAATTVNHLLEASY
                     NEHHLKKRLQEQLFLVVEEGNDIVGFANFIYGEELYLSAHYVKPESQHTGYGTALLNE
                     GLSRFEDKFEGVYLEVDNKNEEAVAYYKEQGFTILRSYQPEMYGEKLDLALMYKAF"
     gene            1546..2841
                     /locus_tag="P6L01_002393"
     CDS             1546..2841
                     /locus_tag="P6L01_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDP3031433.1"
                     /translation="MTNAYVDLKLVEEKVFKDPIHRYIHVEDQLIWDLIKTKEFQRLR
                     RIRQLGTLYLSFHTAEHSRFGHSLGVYEIVRRLIDESFIGHDAWDNKDRPLALCAALL
                     HDLGHGPFSHSFEKIFNTDHEAYTQAIITGDTEVNAVLRKVSPEFPREVAEVINKTHH
                     NKLVISMISSQIDADRMDYLQRDAYFTGVSYGAFDMERILRLMRPSKDEVLIKESGMH
                     AVENFIMSRYQMYWQIYFHPVSRGGEVLLNNCLKRAKQLYNEGYEFKLHPHDFIPFFE
                     ETVTIEQYVELDEAVVTYYLEKWTKEDDAILSDLASRFINRDLFKYIPFDGSIITISE
                     LQELFEAGGINPDYYFVSEAFSDLPYDYDRPGSNRKPIHLLRQDGTIREISNQSLVIH
                     SITGINRQDYKLYYPREMVAKIKDKTIREAIENLINELN"
     gene            2869..>3377
                     /locus_tag="P6L01_002394"
     CDS             2869..>3377
                     /locus_tag="P6L01_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwhD family protein"
                     /protein_id="HDP3031434.1"
                     /translation="MEVISLSEKKGFNFNIIKNDPLDGHKGTNIGSISLDNIAPVFID
                     VANKEAFIDIGGMHARAKVEKGVKWITDKAAVEGDEAKEYWLCWVTTERNEQGPYYAG
                     LTACYLLVNKAIRRGYKSMPEHVNMMDKSMKHHIIIDQIGDENKAILKDFLMNHDEGM
                     WKLSSDALH"
BASE COUNT         1205 a          461 c          663 g         1048 t
ORIGIN      
        1 ctagtcaggg gccccaacac agagaatttc gaaaagaaat tctacaggca atgcaagttg
       61 gggtgtgggc cccaacacag aagctgacga aaagtcagct tacaataatg tgcaagttgg
      121 ggtgggacga cgaaataaat ttttcgaaaa tatcatttct gtcccactcc catcacaaac
      181 gacgttatat aaggcatgac gaaatttcgt caaatttata taaaagttgt aagcgtttta
      241 ctattcggta ggcaaagctt atgatagtat aattaggtaa cgaaaaacac agatatttat
      301 taatcaaaag tgaggttagg ctaaaatgat tactgttttg tttggtggga gtagaccaaa
      361 cggtaatact gcacaattaa caaaattcgc tttgcaagat ttagagtatc aatggattga
      421 cgtgacacaa catcagttta aaccgatacg tgacgtgaga catacagcag agactattac
      481 ttcatatgac gatgactatt tgccgattct agataaaata ttggctagtg atacaattat
      541 ttttgcatca ccagtgtatt ggtatagcat ttcagcacca ttgaaagcat ttatcgaaca
      601 ttggtcagaa acattacaag ataaacgata tcctaatttt aaggcacaaa tggccgaaaa
      661 ggattttaga gttattttag ttggtggaga ttgtccaaaa ataaaagcga agccagcaat
      721 tacgcaaatg aaatatagtt tagacttttt aggtgccact ttaaatggtt atattattgg
      781 aactgctgaa aagcctggtg acatcatgaa agacaactat gccttagcac gtgcaactga
      841 gtggaatagt atattgcaat aagacatacc tcatactaaa cgaagtgatt ttttgcttcg
      901 ttatttttat ttttaatttt aacggaaaat ataaatatat aaattgaata gacaatcatt
      961 gtttattaag tattgagcaa gttgtggtat aatgccgtta ttaaaacttg ttaataaggg
     1021 gagaaagtca tgttcaaggt aagacaagca actgaaaaag atgttgttca aattagagat
     1081 gtcgcaacta aagcttggtt taatacatac ttaaatatat acgctgcgac aacagttaat
     1141 cacttgttag aagcttcata taatgaacat catttaaaga aaagacttca agaacaatta
     1201 ttcttagtcg ttgaagaagg taatgacatc gttggctttg ctaactttat ttacggtgaa
     1261 gaattatatt tatcagctca ttatgttaaa ccagaatcgc aacatacagg ttatggtaca
     1321 gcattgttaa atgaaggatt atcacgtttt gaagataaat ttgaaggtgt ttacttagaa
     1381 gtagataata aaaatgaaga agcagtagct tactataaag agcaaggttt tacaatctta
     1441 cgctcttatc agccagaaat gtatggcgaa aagttagact tagcacttat gtacaaagca
     1501 ttttaagtaa attaaatatt atattaaacg aaaaggaggg gaataatgac taacgcatat
     1561 gtagatttaa aattagtaga agaaaaagtt tttaaagatc cgatacatcg atatattcat
     1621 gttgaagatc aattgatatg ggatttaatt aaaactaagg aattccaaag gttacgtcga
     1681 attagacaac taggaacact gtacctatct tttcacacag cagaacatag tcgctttgga
     1741 cattctttag gtgtgtatga aatagttaga cgattaattg atgagtcatt tattggtcat
     1801 gatgcatggg acaataaaga tagaccgttg gcattatgtg ctgcattatt acatgattta
     1861 ggacatggtc cattttcaca tagttttgaa aaaatattta atacagacca tgaagcatac
     1921 acacaagcga ttattactgg agatactgag gtgaatgctg tattacgtaa agtgtcgcct
     1981 gagtttccaa gagaagttgc ggaagtaatt aataaaacgc atcataataa attggtcatt
     2041 tcgatgattt cgtcacaaat cgatgcggat agaatggatt atttacaacg tgatgcgtat
     2101 ttcacaggtg tatcatatgg tgcttttgat atggagcgta ttttaagatt aatgcgacct
     2161 tctaaagatg aagtactaat caaagaaagt ggtatgcatg cagttgaaaa ctttattatg
     2221 agtcgttatc aaatgtattg gcaaatttac ttccacccag ttagtcgtgg tggagaagtg
     2281 ctgcttaata attgtttgaa acgcgcaaaa cagctttata atgaaggcta tgaatttaag
     2341 ttgcatccac atgattttat tccatttttt gaagagacag ttacgattga acaatatgtt
     2401 gaactcgatg aagcggtagt tacgtattat ttggaaaaat ggacaaaaga agatgatgcc
     2461 attttaagtg atttagcaag tcgatttatt aatcgagact tatttaaata tattccattt
     2521 gatggctcaa ttattacaat atcagaactg caagaactgt ttgaagcagg tggtattaat
     2581 ccagattatt attttgtgag tgaagcattt tcggatttgc catatgacta tgatcgaccg
     2641 gggtcaaatc gcaaaccgat tcatttatta agacaagatg gtacgattag agaaataagc
     2701 aatcaatcat tagtcattca tagtattaca ggcattaatc gccaagacta taaattatat
     2761 tatcctagag aaatggttgc aaagattaaa gataagacaa ttagagaagc tattgaaaat
     2821 ttgattaatg agcttaatta aacagggcta aaattgttat cgttaaatat ggaggttata
     2881 tcattgtctg agaaaaaagg ctttaatttt aatatcataa aaaatgaccc tctagatggt
     2941 cataaaggta caaatattgg ttcaattagc ttagacaata ttgcaccagt ttttatcgat
     3001 gttgctaaca aagaagcatt tattgatatt ggaggcatgc atgctcgtgc caaagttgaa
     3061 aaaggtgtga aatggattac tgataaagct gctgttgaag gcgatgaagc taaagaatat
     3121 tggttgtgtt gggtaacaac agaacgtaat gaacaaggac catattacgc tggtttaaca
     3181 gcgtgctatt tattagtgaa taaagcaatt cgtcgtggtt ataaaagtat gcctgaacat
     3241 gttaatatga tggataaatc aatgaaacat catattatca ttgatcaaat tggtgacgag
     3301 aataaagcta ttttaaaaga ctttttaatg aaccatgatg aaggtatgtg gaagctttct
     3361 tctgatgctt tacatca
//
LOCUS       DAOBBH010000216         3343 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.216, whole genome shotgun sequence.
ACCESSION   DAOBBH010000216 DAOBBH010000000
VERSION     DAOBBH010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.216"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..933)
                     /locus_tag="P6L01_002395"
     CDS             complement(<1..933)
                     /locus_tag="P6L01_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDP3031435.1"
                     /translation="MIDTIKVNNKTIPWLYVERGFEIPSFNYVLKTENVDGRSGSIYK
                     GRRLESYSFDIPLVVRNDYLSHNGIKTHDDVLNELVKFFNYEEQVKLQFKSKDWYWNA
                     YFEGPIKLHKEFTIPVKFTIKVVLTDPYKYSVTGNKNTAISDQVSVVNSGTADTPLIV
                     EARAIKPSSYFMITKNDEDYFMVGDDEVTKEVKDYMPPVYHSEFRDFKGWTKMITEDI
                     PSNDLGGKVGGDFVISNLGEGYKATNFPDAKGWVGAGTKRGLPKAMTDFQITYKCIVE
                     QKGKGAGRTAQHIYDSDGKLLASIGYENKYHDRKI"
     gene            complement(930..>3343)
                     /locus_tag="P6L01_002396"
     CDS             complement(930..>3343)
                     /locus_tag="P6L01_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001799555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDP3031436.1"
                     /translation="IKFFSSLFTGDWRGVWDAIVMILKGVVQLIWNLIQLWFVGKILG
                     VVRYFGGLLKGLIAGIWDVIKSIFSKSLSAIWNATKSIFGFLFNSVKSIFTNMKNWLS
                     NTWSSIRTNTIGKAQSLFSGVKSKFTNLWNATKEIFSNLRNWMSNIWNSIKDNTVGIA
                     SRLWSKVRGIFTNMRDGLQSIISKIKSHIGGMVDAIKKGLNKLIDGLNWVGGKLGMDK
                     IPKLHTGTEHTHTTTRLVKNGKIARDTFATVGDKGRGNGPNGFRNEMIEFPNGKRVIT
                     PNTDTTAYLPKGSKVYNGAQTYSMLNGTLPRFSLGTMWKDIKSGASSAFNWTKDQIGK
                     GTKWLGDKVGDVLDFIENPGKLLNYILEAFGIDFNSLTKGMGIAGDITKAAWSKIKKS
                     ATDWIKENLEAMGGGDLVGGILDPDKINYHYGRTAAYTAATGRPFHEGVDFPFVYQEV
                     RTPMGGRLTRMPFMSGGYGNYVKITSGVIDMLFAHLKNFSKSPPSGTMVKPGDVVGLT
                     GNTGFSTGPHLHFEMRRNGRHFNPEPYLRNAKKKGRLSIGGGDATSGSGATYASRVIR
                     QAQSILGGRYKGKWIHDQMMRVAKRESNYQSNAVNNWDINAQRGDPSRGLFQIIGSTF
                     RANAKRGYTNYNNPVHQGISAMQYIVRRYGWGGFKRAGDYAYATGGKVFDGWYNLGED
                     GHPEWIIPTDPARRNDAMKILHYAAAEVRGKKASKNKRPSQLSDLNGFDDPSLLLKMI
                     EQQQQQIALLLKIAQSNDVIADKDYQPIIDEYAFDKKVNASIEKRERQESTKVKFRKG
                     GIAIQ"
BASE COUNT          980 a          722 c          477 g         1164 t
ORIGIN      
        1 tatttttcta tcatgatatt tattttcata accaatagaa gcaagtaact taccatcact
       61 atcataaata tgttgtgctg ttcttccggc acctttacct ttttgttcaa caatacattt
      121 ataggtaatt tgaaaatctg tcatcgcttt agggagccct cgtttcgtgc cagcaccaac
      181 ccaacctttt gcatcaggaa aattagttgc tttatatcct tcgccaagat tggatatcac
      241 aaagtcaccg ccgaccttac cacctaagtc attacttgga atatcttcag taatcatctt
      301 agtccaacct ttgaaatcac gaaactcact atgataaaca ggaggcatgt aatccttaac
      361 ttctttggtt acctcatcat caccaaccat aaaataatct tcatcatttt tagtaatcat
      421 aaagtaacta gatggtttaa ttgctcgggc ttcaacaatt aaaggagtgt cagcagtccc
      481 actatttaca actgaaactt ggtctgaaat cgcagtattt ttatttcctg ttactgaata
      541 tttgtaaggg tctgttagta ctactttgat agtgaactta acaggtattg taaattcttt
      601 gtgcagcttt attggtcctt cgaaataagc gttccagtac caatctttag atttgaattg
      661 taatttaact tgttcctcgt agttaaaaaa ctttactaat tcattcaaga cgtcatcatg
      721 tgttttaatg ccgttgtgag ataaatagtc attacgtacc accaaaggta tatcaaaact
      781 ataagattca agcctacgcc ctttatatat agaccccgaa cgtccatcta cattttctgt
      841 ttttaaaaca taattaaaag agggtatttc aaaccctctt tcgacataca accaaggaat
      901 tgttttgttg ttcactttaa tagtgtctat cattgaatag caattcctcc ttttctaaac
      961 tttacttttg ttgattcttg cctttctcgc ttttctatag acgcgttcac ctttttatca
     1021 aaagcgtatt cgtcaataat cggctgataa tctttatctg caatcacatc gttagattgt
     1081 gctattttca gtaataaagc tatttgttgt tgctgttgtt caatcatttt caataataag
     1141 ctaggatcat caaacccgtt taagtctgat aattggctag gacgcttatt tttactcgct
     1201 tttttccctc ttacttctgc tgctgcataa tgcaaaatct tcattgcatc atttctacga
     1261 gctggatctg ttggaataat ccattctgga tgaccgtctt cacctaagtt ataccaacca
     1321 tcaaaaactt ttccacctgt agcatatgcg taatcaccag cacgtttaaa accaccccaa
     1381 ccatatcgtc taacaatgta ctgcattgct gagatacctt gatgtactgg attattataa
     1441 ttagtgtacc ctcgtttagc gttagctcta aaagttgagc cgataatttg gaataatcct
     1501 ctagacgggt ctcctctttg agcattaata tcccaattat tcactgcatt tgattgatag
     1561 ttgctttcac gctttgcaac tcgcatcatc tggtcatgaa tccacttacc tttataacgt
     1621 cctcctaaaa tactttgcgc ttgtcggatt actcggctgg cataagttgc accacttcca
     1681 gaagtagcat caccgccacc aattgataac ctaccttttt tctttgcatt tcttaaatat
     1741 ggttcagggt taaaatggcg tccgtttctc ctcatttcaa aatgtaagtg tggtcctgta
     1801 ctaaatccgg tattaccagt taaaccaaca acatcgccgg gctttaccat cgtgccacta
     1861 ggtggtgatt tgctaaagtt tttcaaatgc gcaaatagca tatcgataac gccactagta
     1921 atttttacat agttaccata accaccagac ataaacggca ttcttgtaag tctaccaccc
     1981 ataggcgttc taacttcttg atatacaaat ggaaaatcga caccttcatg aaatggtctt
     2041 ccagttgcag cggtataagc tgcggtacgt ccataatgat aattaatttt gtcagggtct
     2101 aatattccgc cgactaaatc gccaccgccc atagcttcta aattttcttt tatccaatca
     2161 gtagcacttt tcttaatctt agaccatgca gcttttgtta tgtcgcctgc aattcccatt
     2221 cctttagtta aagaattgaa atcaattcca aaagcttcaa gtatataatt taaaagtttt
     2281 cctggatttt caataaaatc taaaacatcg ccaactttat cgccaagcca tttggtacct
     2341 ttacctattt gatcttttgt ccagttaaat gctgatgatg caccggactt aatatctttc
     2401 cacatagtac ctaaactaaa tcttggaaga gttccgttta acattgaata agtttgtgca
     2461 ccgttatata cttttgagcc tttaggtaaa taagcagtag tatctgtatt tggtgtgatt
     2521 acacgtttac cattagggaa ttcaatcatt tcgtttctaa aaccatttgg accatttccg
     2581 cgtcccttat ctccaactgt agcgaatgtg tcacgtgcaa tcttaccgtt cttaactaat
     2641 cttgtagtag tatgtgtgtg ctctgtacca gtatgtaact taggtatttt atccatgccc
     2701 aacttaccac cgacccagtt taaaccgtcg attaatttat taagtccttt tttaatagca
     2761 tctaccatac cgccgatatg acttttaatt ttgctgataa tactttgtaa gccgtcacgc
     2821 atatttgtaa aaattccacg taccttactc cataaacggc tagcaattcc taccgtatta
     2881 tctttaatgg aattccaaat atttgacatc caatttctta aattactaaa aatttctttc
     2941 gtcgcattcc ataaattagt aaattttgat ttgacgccac taaataatga ctgcgctttt
     3001 cctatcgtat tcgtacggat actactccaa gtattagata accaattttt catattcgtg
     3061 aaaattgatt tgacactatt aaataagaat ccaaaaatac tttttgtcgc attccaaatt
     3121 gctgataaag atttactgaa tatacttttt attacgtccc aaatacctgc tattaatcct
     3181 tttagcaatc cgccaaagta cctaacaacg ccaagtattt tgcctacaaa ccataattga
     3241 attaaattcc atattaattg aacgactcct ttaagaatca taacaatcgc atcccaaact
     3301 cctcgccaat ctccagtaaa taaacttgag aagaacttaa tta
//
LOCUS       DAOBBH010000217         3224 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.217, whole genome shotgun sequence.
ACCESSION   DAOBBH010000217 DAOBBH010000000
VERSION     DAOBBH010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.217"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(169..438)
                     /gene="rpsO"
                     /locus_tag="P6L01_002397"
     CDS             complement(169..438)
                     /gene="rpsO"
                     /locus_tag="P6L01_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDP3031437.1"
                     /translation="MAISQERKNEIIKEYRVHETDTGSPEVQIAVLTAEINAVNEHLR
                     THKKDHHSRRGLLKMVGRRRHLLNYLRSKDIQRYRELIKSLGIRR"
     gene            complement(553..1524)
                     /gene="ribF"
                     /locus_tag="P6L01_002398"
     CDS             complement(553..1524)
                     /gene="ribF"
                     /locus_tag="P6L01_002398"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDP3031438.1"
                     /translation="MKVIEVTHPIQSKQYITEDVAMAFGFFDGMHKGHDKVFDILNEI
                     AEARSLKKAVMTFDPHPSVVLNPKRKRTTYLTPLSDKIEKISQHDIDYCIVVNFSSRF
                     ANVSVEDFVENYIIKNNVKEVIAGFDFTFGKFGKGNMTVLQEYDAFNTTIVSKQEIEN
                     EKISTTSIRQDLINGELQKANDALGYIYSIKGTVVQGEKRGRTIGFPTANIQPSDDYL
                     LPRKGVYAVSIEIGTENKLYRGVANIGVKPTFHDPNKAEVVIEVNIFDFEDNIYGERV
                     TVNWHHFLRPEIKFDGIDPLVKQMNDDKSRAKYLLAVDFGDEVAYNI"
     gene            complement(1539..2456)
                     /gene="truB"
                     /locus_tag="P6L01_002399"
     CDS             complement(1539..2456)
                     /gene="truB"
                     /locus_tag="P6L01_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDP3031439.1"
                     /translation="MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAG
                     VLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQTGDTLETKGVHSADFNKDDI
                     DRLLENFKGVIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
                     KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQI
                     KELHEQDSLQNKLFPLEYGLKGLPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVF
                     IDDDSEKVLAIYMVHPTKESEIKPKKVFN"
     gene            complement(2625..2975)
                     /gene="rbfA"
                     /locus_tag="P6L01_002400"
     CDS             complement(2625..2975)
                     /gene="rbfA"
                     /locus_tag="P6L01_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDP3031440.1"
                     /translation="MSSMRAERVGEQMKKELMDIINNKVKDPRVGFITITDVVLTNDL
                     SQAKVFLTVLGNDKEVENTFKALDKAKGFIKSELGSRMRLRIMPELMYEYDQSIEYGN
                     KIERMIQDLHKQDR"
BASE COUNT         1005 a          599 c          407 g         1213 t
ORIGIN      
        1 gattattact ctaggtgtac ttatatcatc tttatctcat tgtaacctta ttattcactc
       61 aatattagct taacatataa taaatgttaa tatttgtaaa aagctttttt aatataaatt
      121 aaggttggaa cataaatatg ccccaacctc aaagacgtta tattaagatt aacgacggat
      181 accaagtgat ttaattaatt cacggtaacg ttgaatatct ttactacgta agtagtttaa
      241 taaatgtcta cgacgaccta ccatttttaa taatccacga cgtgaatggt ggtctttttt
      301 gtgtgtacgt aagtgttcgt ttactgcgtt gatttctgca gtaagtacag cgatttgtac
      361 ttctggtgaa ccagtatcag tttcgtgtac acggtattct ttaatgattt cgtttttacg
      421 ttcttgtgaa attgccataa tcaatttcct ccttaaaatg tttgaatact ccttaatccg
      481 agttaggcgt tggagattcg acctactaag aaaaaggtgt ggtatagatt gtttatataa
      541 ctatacgcaa ctctagatat tataagctac ttcatcacca aaatcaactg ctaataaata
      601 tttagcacgc gatttatcat cgttcatttg tttaactaat gggtcgatac catcaaattt
      661 aatctcagga cgtaagaaat gatgccaatt cacagtcact cgttcaccat aaatattatc
      721 ctcaaagtca aagatattca cttcgatgac aacttctgct ttgttaggat catgaaatgt
      781 tggctttaca cctatgttag ctacccctcg atataattta ttttcagtgc cgatttcaat
      841 actaacagca taaacacctt tacgaggtaa caaataatca tcactaggtt gaatgttagc
      901 tgttgggaag ccaatagttc ttcccctttt ttcaccttgc actacagtgc ctttaataga
      961 ataaatatag cctaaagcat cattcgcttt ttgcaactca ccattgatta aatcttgacg
     1021 aatagaagtt gtagaaattt tttcattttc aatttcttgt ttactcacaa ttgtcgtatt
     1081 aaacgcatca tattcttgaa gaacagtcat attacctttt ccaaatttac caaaagtaaa
     1141 atcaaaacca gcaatgactt cttttacatt atttttaatt atataatttt caacaaaatc
     1201 ttcaacgctc acattagcaa acctagatga aaaattaacc actatacaat aatcaatatc
     1261 atgttggcta attttttcga ttttatctga aagtggtgtt aaatacgttg ttcgttttct
     1321 tttaggattc aacacgacag acggatgcgg atcaaatgtc atcaccgctt tttttaaact
     1381 gcgtgcctca gctatttcat ttaatatatc aaagactttg tcatgacctt tatgcatgcc
     1441 atcgaaaaat ccgaatgcca ttgcaacatc ctctgtaata tactgtttag attgtatagg
     1501 atgtgtcact tctatgactt tcataaattc tatctccttt aattaaagac ttttttaggt
     1561 ttaatttctg attcttttgt agggtgtacc atataaattg ctaatacttt ttctgaatca
     1621 tcatcaataa atacaatttg gtctttaatt ttgttatcaa attcattttt attaaatttc
     1681 tgcccattta aaatacgttt ttttatgtgc gaatctttaa ttttaatgct tggcaaaccc
     1741 tttaatccat attctaaagg aaacaattta ttttgcaatg aatcctgctc atgaagttct
     1801 tttatttgtt ctaatgtaag gctatctttc aacacaaatc caccagactc gattcgtgtt
     1861 aatttcgaca tatgtgccgg aaagcctaat ttcacaccaa tatcagttgc tagcgttcta
     1921 atatatgtac ctttaccaca gatgacgcgt attttaaaat gacactcatt ttctttaaaa
     1981 tctaattcag atatacgccc aatgtcttta atattaactt tacgctttgg tctttcaact
     2041 gtttcattat tacgcgcata ttcatataat tttttgccat tcaccttgac tgatgaatac
     2101 attggcggaa tttgttcaat aacaccttta aaattttcta acaatcggtc aatatcgtcc
     2161 ttattaaaat ctgctgagtg tacacctttt gtttccaatg tatcacccgt ttgatcttca
     2221 gtcgttgtac ttcttcctat cgatacagtt gcttcataag ctttgcccat atccataaca
     2281 taatcactaa ctctcgttgc attacctata cataccggta acacgcctgc aacttcggga
     2341 tcaagcgtac ccgtgtgacc tattttttta gtttttaata ttttacgcaa tttgaataca
     2401 acgtcatgac ttgttaaacc gcgctcttta tatactggta atatcccatt atacatatag
     2461 tcaccttctt ttttaaaata tattttaata gcgtatcaac tgaatttatt cataaaaaaa
     2521 ctggaaaatg ttaactattt ctacacatct atgtattaac tgtttaatgt taatataccg
     2581 atattaagaa attatcaaac attttccaga tacctaacac taaattatct atcttgtttg
     2641 tgtaaatctt gaatcattcg ttcaatttta ttaccatatt cgattgattg atcatattca
     2701 tacattaatt ccggcataat acgtaatcgc attctagaac ctaattcaga cttaatgaag
     2761 ccttttgctt tatcaagtgc tttaaatgta ttttctactt ctttatcgtt acctaataca
     2821 gttaaaaata ctttagcctg cgataaatca tttgttaaaa caacatctgt aattgtaata
     2881 aaaccaactc gaggatcttt gactttattg ttgatgatat ccattaattc cttcttcatt
     2941 tgttcaccaa cacgctctgc tctcatactg ctcattcttg tcacctcttt atctttaagt
     3001 tcaattgtta tttgcatttt ttagtgtaac aatgcttaaa caattatttt agctcacacg
     3061 ttattttaat aaattaagcc tataaaatca aataaagttt gccgtaagat aggcatttca
     3121 acagaaaatt ttccaataat agtcgacatt atattttgtc attaaaaatc attctaggcc
     3181 accacaaaat tttatacaaa atcatgtcca tataacgtaa cttt
//
LOCUS       DAOBBH010000218         3212 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.218, whole genome shotgun sequence.
ACCESSION   DAOBBH010000218 DAOBBH010000000
VERSION     DAOBBH010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.218"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(201..1103)
                     /gene="cvfB"
                     /locus_tag="P6L01_002401"
     CDS             complement(201..1103)
                     /gene="cvfB"
                     /locus_tag="P6L01_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HDP3031441.1"
                     /translation="MALDKDIVGSIEFLEVVGLQGSTYLLKGPNGENVKLNQSEMNDD
                     DELEVGEEYSFFIYPNRSGELFATQNMPDITKDKYDFAKVLKTDRDGARIDVGLPREV
                     LVPWEDLPKVKSLWPQPGDHLLVTLRIDRENHMYGRLASESVVENMFTPVHDDNLKNE
                     VIEAKPYRVLRIGSFLLSESGYKIFVHESERKAEPRLGESVQVRIIGHNDKGELNGSF
                     LPLAHERLDDDGQVIFDLLVEYDGELPFWDKSSPEAIKEVFNMSKGSFKRAIGHLYKQ
                     KIINIETGKITLTKKGWSRIDSKE"
     gene            1250..2851
                     /locus_tag="P6L01_002402"
     CDS             1250..2851
                     /locus_tag="P6L01_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDP3031442.1"
                     /translation="MLQVTDVSLRFGDRKLFEDVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGELDSQTGHVSLGKNERLAVLKQDHYAYEDERVLDVVIKGHERLYEVMKEKDEI
                     YMKPDFSDEDGIRAAELEGEFAEMNGWNAEADAANLLSGLGIDPTLHDKKMAELENNQ
                     KIKVLLAQSLFGEPDVLLLDEPTNGLDIPAISWLEDFLINFDNTVIVVSHDRHFLNNV
                     CTHIADLDFGKIKVYVGNYDFWYQSSQLAQKMAQEQNKKKEEKMKELQDFIARFSANA
                     SKSKQATSRKKQLEKIELDDIQPSSRRYPFVKFTPEREIGNDLLIVQNLSKTIDGEKV
                     LDNVSFTMNPNDKAILIGDSEIAKTTLLKILAGEMEPDEGSFKWGVTTSLSYFPKDNS
                     EFFEGVNMNLVDWLRQYAPEDEQTETFLRGFLGRMLFSGEEVKKKASVLSGGEKVRCM
                     LSKMMLSSANVLLLDEPTNHLDLESITAVNDGLKSFKGSIIFTSYDFEFINTIANRVI
                     DLNKQGGVSKEIPYEEYLQEIGVLK"
BASE COUNT         1074 a          528 c          546 g         1064 t
ORIGIN      
        1 tacatatcaa aaaagctaat ttccatcaaa taattaatag aaatcagctt ttttacattg
       61 cctaagaact taatgtccca gcctattcgt ttgaattatt taatttttta ctttgaaagg
      121 aatgtttaaa aaactacttc atctgtccga cttaatattg aatataaaaa acacgcatcc
      181 tacgacacgt gtaaaaatga ttattctttt gagtcaattc gactccaacc ttttttagtt
      241 aaagtgattt tacctgtttc tatattaata atcttctgtt tatataagtg accgattgca
      301 cgtttgaatg aacctttact catattgaat acttctttaa tcgcttcagg gcttgatttg
      361 tcccagaatg gtaattcacc atcatattca actagtaaat caaagatgac ttggccgtca
      421 tcgtctaaac gttcatgtgc aagtggtaaa aatgaaccat ttaactcacc tttatcatta
      481 tgcccgataa ttctaacttg aacagattca cctaatcttg gttcagcttt acgttctgat
      541 tcatgtacga aaattttgta acctgattcg cttaataaga agctaccaat tcgtaatacg
      601 cggtaaggtt tggcttcaat gacttcgttt tttaaattat catcgtgtac aggtgtaaac
      661 atattttcta caacagattc actcgctaaa cgtccataca tatgattctc acggtcaatt
      721 cgtaatgtaa ctagcaaatg atcaccaggt tgtggccata gtgatttcac ttttggtaaa
      781 tcttcccatg gtactaacac ttcacggggt aaaccaacat ctatacgtgc cccatcgcga
      841 tccgttttaa gtactttagc aaaatcatat ttatctttcg taatatcagg catattttga
      901 gttgcaaata attcacctga acggtttgga taaatgaaga aactatattc ttcacctact
      961 tctaattcat catcatcgtt catttctgat tggtttaact ttacgttttc accgtttggt
     1021 ccttttaaaa ggtaagttga gccttgtaac cctactactt caaggaattc tatagaacct
     1081 actatatctt tgtctaatgc catgttattc tccttcgatt cttgtattct ttattattat
     1141 aacatgtgtg agtcattcta tcattacaac ttatatgagg tcgaaactgt gacgataata
     1201 tgatataatt cttccgttag aatatcgaat gaaaaaaagg agaaaatgca tgttacaagt
     1261 aactgatgtg agtttacgtt ttggagatcg taaactattt gaagatgtaa atattaaatt
     1321 tacagaaggt aattgttatg gattaattgg tgcgaatggt gcaggtaaat caacattctt
     1381 aaaaatatta tctggtgaat tagattctca aacaggacat gtttcattag gtaaaaatga
     1441 acgtctagct gttttaaaac aggaccacta tgcttatgaa gatgaacgcg tgcttgatgt
     1501 tgtaattaaa ggtcacgaac gtctttatga ggttatgaaa gaaaaagatg aaatctatat
     1561 gaagccagat ttcagtgatg aagatggtat ccgtgctgct gaacttgaag gtgaatttgc
     1621 agaaatgaat ggttggaatg ctgaagctga tgctgctaac cttttatctg gtttaggtat
     1681 cgatccaact ttacacgata aaaaaatggc tgaattagaa aacaaccaaa aaattaaagt
     1741 attattagcg caaagtttat tcggtgaacc agacgtacta ttactggatg agcctactaa
     1801 cggtctcgat attccagcaa tcagttggtt agaagatttc ttaattaact ttgataatac
     1861 tgttatcgta gtatcgcatg accgtcattt cttaaataat gtatgtactc atatcgctga
     1921 tttagacttc ggtaaaatta aagtttatgt tggtaactat gatttttggt atcaatctag
     1981 tcagttagct caaaagatgg ctcaagaaca aaacaagaaa aaagaagaaa aaatgaaaga
     2041 gttacaggac tttattgcac gtttctcagc taacgcttct aaatctaaac aagcaacaag
     2101 tcgtaaaaaa caacttgaga aaattgaatt agatgatatt caaccatcat caagaagata
     2161 tcctttcgtt aaattcacgc ctgagcgtga gattggtaac gacttattaa tcgttcaaaa
     2221 tctttctaaa acaattgacg gcgaaaaagt attagataat gtatcattca caatgaatcc
     2281 aaatgataaa gcgattttaa ttggagatag tgaaattgca aaaacaacat tacttaaaat
     2341 attagctggc gaaatggaac cagacgaagg ttcatttaaa tggggtgtta ctacatcatt
     2401 aagttacttc cctaaagata actcagagtt ctttgagggt gtaaatatga atctcgttga
     2461 ttggttaaga caatatgctc ctgaagatga acaaacagaa acatttttac gtggtttctt
     2521 aggtcgtatg ttatttagtg gtgaagaagt taagaaaaaa gctagtgtgc tttcaggtgg
     2581 agaaaaagta cgttgtatgc taagtaaaat gatgttatca agtgcgaatg tacttttact
     2641 tgacgaacct actaaccact tagacttaga aagtattact gctgtcaatg atggtcttaa
     2701 atcatttaaa ggttctatca tctttacttc ttatgacttc gaatttatca acacgattgc
     2761 aaaccgtgtt atcgatttaa ataaacaagg cggcgtttca aaagaaattc catatgaaga
     2821 atacttgcaa gaaatcggcg ttttaaaata aacataatat gtagaatttc ttttcgaaaa
     2881 tctctgtatt gaggcccgac caacttgcac attattgtaa gttggcttac tgtcaccttc
     2941 tatattggga cttattccgc aacttgtatt gtttgtagaa attaggaatc caatttctct
     3001 atgtgttgca ccgacaagga ttgaaaaaag ctagctacaa agtcattttc attcagtcaa
     3061 ctactgtcaa tataacattg cagggtctag ggcattgatt catgcccgag actatattca
     3121 atttaataga cattaactgg tcgtgaggct gggacataaa tccctaaaaa aacagcagta
     3181 agataatttt caattagaaa atatcttact gc
//
LOCUS       DAOBBH010000219         3200 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.219, whole genome shotgun sequence.
ACCESSION   DAOBBH010000219 DAOBBH010000000
VERSION     DAOBBH010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.219"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(413..1249)
                     /locus_tag="P6L01_002403"
     CDS             complement(413..1249)
                     /locus_tag="P6L01_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDP3031443.1"
                     /translation="MDLASKYFNGVNWRYIDHSSGLEPMQSFAFDDTFCESVGKDISD
                     NVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNI
                     SLMFKGQTETTIDEAFTVMYLLISKMFEDENVDIDTMEIEHSYCPGKFDLSIDGKKFA
                     GISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASL
                     ETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQ
                     MQ"
     gene            complement(1252..2238)
                     /gene="pta"
                     /locus_tag="P6L01_002404"
     CDS             complement(1252..2238)
                     /gene="pta"
                     /locus_tag="P6L01_002404"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDP3031444.1"
                     /translation="MADLLNVLKDKLSGKNVKIVLPEGEDERVLTAATQLQATDYVTP
                     IVLGDETKVQSLAQKLDLDISNIELINPATSELKAELVQSFVERRKGKATEEQAQELL
                     NNVNYFGTMLVYAGKADGLVSGAAHSTGDTVRPALQIIKTKPGVSRTSGIFFMIKGDE
                     QYIFGDCAINPELDSQGLAEIAVESAKSALSFGMDPKVAMLSFSTKGSAKSDDVTKVQ
                     EAVKLAQQKAEEEKLEAIIDGEFQFDAAIVPGVAEKKAPGAKLQGDANVFVFPSLEAG
                     NIGYKIAQRLGGYDAVGPVLQGLNSPVNDLSRGCSIEDVYNLSIITAAQALQ"
     gene            2406..3158
                     /locus_tag="P6L01_002405"
     CDS             2406..3158
                     /locus_tag="P6L01_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent peroxidase"
                     /protein_id="HDP3031445.1"
                     /translation="MSQAAETLDGWYSLHLFYAVDWASLRIVPKDERDALVTEFQSFL
                     ENTATVRSSKSGDQAIYNITGQKADLLLWFLRPEMKSLNHIENEFNKLRIADFLIPTY
                     SYVSVIELSNYLAGKSDEDPYENPHIKARLYPELPHSDYICFYPMNKRRNETYNWYML
                     TMEERQKLMYDHGMIGRKYAGKIKQFITGSVGFDDFEWGVTLFSDDVLQFKKIVYEMR
                     FDETTARYGEFGSFFVGHLINTNEFDQFFAIS"
BASE COUNT         1027 a          589 c          467 g         1117 t
ORIGIN      
        1 gattagtata aaactaactt tcgtttactt caaagtttaa atcttatcga gtgatatttc
       61 agattcttta tctttttata aaatagccct acaatttata attttccacc ctaactataa
      121 tactacaaat aataattgga atatatagat ttactactaa agtattagaa catttcaata
      181 gaaggtcgtt tctttcatag tcatacgcat tatatatacc ctattctcaa tctatttaat
      241 acgtaaaaca tgaaattttc ttattaaatt tattatttcc atcatatcat tacttttaat
      301 ttaatgatgt tcaatttaaa tattaggtca ataacatatt tatgcttttt atggatactt
      361 tcaaaaataa cagccccaaa cgataacttg aaaggggctg ttaaatattt aactattgca
      421 tttggtctat cattttcttg tttctttcaa tcattttatc aaaatacgta tcgtatcttt
      481 gccattcttc ttgagtaatg ggcgtcatat ttaatacacc gccaagatct ttgattgcat
      541 ataatagtaa aaacattaca tcttgaacag taatcgtttt gttaagcaat gtctctaatg
      601 aggccataca agatggttca atttcaggat atttaaattt agtcacttca ccttttaaag
      661 catgttcata aaatgtttgc atcatcaatg cacgttctga accagagcct tcaacacaaa
      721 gataaatttg tacagcaata ccgcctctaa ctcttcgttg cgatatgcct gcaaatttct
      781 taccatcgat acttaagtca aattttcctg ggcaataaga atgttcaatt tccatcgtat
      841 caatatcaac attctcatct tcgaacattt tgctaattaa gaggtacatc acagtaaacg
      901 cttcatcaat cgttgtttct gtttgtcctt tgaacatcag cgatatattt aatacacctt
      961 gatctagaac gacacctaag ccaccagaat ttctaacaat ggcattataa ccaatctcat
     1021 tcgttaaata atcaatgcca tcttttaaaa acggcaatct tgaatcatga ataccaagaa
     1081 taacagtatg ttgatgaatc caagtacgca caacattatc tgatatatct ttgcccacac
     1141 tttcgcaaaa tgtatcatcg aatgcgaaag attgcatagg ttctaatcca gaagaatggt
     1201 cgatatatcg ccagttgacg ccattaaaat atttactcgc taaatccatc gttattgtaa
     1261 ggcttgcgct gctgtaataa ttgaaagatt gtatacatct tcaattgagc agccacgtga
     1321 taagtcattt actggagaat ttaaaccttg taatactgga ccaactgcat catatccacc
     1381 taaacgttgt gcaattttgt aaccaatatt accagcttct aaacttggga atacaaagac
     1441 atttgcatca ccttgtaatt tagcacctgg cgcttttttc tcagcaacac ctggtacaat
     1501 cgcagcatca aattggaatt cgccatcaat gattgcttct aatttttctt cttcagcttt
     1561 ttgttgtgct aatttgacag cttcttgaac ttttgtcacg tcgtctgatt tagcagaccc
     1621 ttttgttgaa aagcttaaca ttgcaacttt tggatccatg ccaaagctta atgctgattt
     1681 tgcactttct actgcaattt ctgcaagtcc ttgtgaatca agttctggat tgattgcaca
     1741 atcaccaaag atgtattgtt catcaccttt aatcataaag aagatacctg atgttcttga
     1801 tacacctggt ttcgttttga tgatttgtaa agctggacgc acagtgtctc ctgttgaatg
     1861 tgctgcacca ctaactaaac catctgcttt accagcataa acaagcattg taccgaagta
     1921 gttcacattg tttaataatt cttgtgcttg ttcttcagtc gctttacctt tacgtcgttc
     1981 aacaaatgat tgaactaatt cagctttcaa ttcacttgtc gcaggattaa ttaattcaat
     2041 attagaaata tcaagatcaa gtttttgcgc taaagattga accttagtct catcacctaa
     2101 cacgattggt gtaacataat ctgttgcttg taattgtgtt gcagctgtta gaacacgttc
     2161 gtcctctcct tcaggtaata cgattttaac gtttttacca gaaagtttgt cttttaatac
     2221 atttaataaa tcagccataa tgtcctcctg taatataaat cttattaatc attcacggta
     2281 taattatacg ccattttatt tcataatgcc acgtcaccaa tttgtaatca taattttatc
     2341 gttacccctt tgctttatga taaaatttat aaagaactga tgatttttga aaaggagcga
     2401 taaacatgag tcaagcagcc gaaacattag atggttggta tagtctacat ttattttatg
     2461 cagttgattg ggcatcatta cgtatagttc caaaggacga acgcgatgca cttgtcactg
     2521 aatttcaatc atttttagaa aatacagcaa ctgtaagatc atcaaaatct ggtgatcaag
     2581 ctatttataa tataactggt caaaaagcag atttgttatt atggttctta cgtcctgaaa
     2641 tgaagtcttt aaatcatatt gaaaatgaat ttaacaaatt gcgcattgct gacttcctaa
     2701 tccctacata ttcatatgta tcagtcattg aattgagcaa ttatttagct ggtaaatctg
     2761 atgaagatcc ttatgagaac cctcatatca aagcaagatt atacccagaa ttaccacatt
     2821 ctgattatat ttgtttctat ccaatgaaca aacgtcgtaa tgaaacttat aactggtaca
     2881 tgttaactat ggaagaacgc caaaaattaa tgtatgacca tggtatgatt ggtagaaaat
     2941 atgctggcaa aatcaaacaa tttattactg gttctgtagg gtttgatgat ttcgaatggg
     3001 gcgtaacatt gttctcagat gacgtattac aattcaaaaa aattgtatac gaaatgcgct
     3061 ttgatgaaac aacagcacga tacggtgaat tcggtagttt ctttgtagga catcttatta
     3121 acacaaacga attcgatcaa ttctttgcga tttcttaata cattggtacg tttatacatt
     3181 gcatgacaaa aaacaattct
//
LOCUS       DAOBBH010000220         3092 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.220, whole genome shotgun sequence.
ACCESSION   DAOBBH010000220 DAOBBH010000000
VERSION     DAOBBH010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.220"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            168..932
                     /locus_tag="P6L01_002406"
     CDS             168..932
                     /locus_tag="P6L01_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDP3031446.1"
                     /translation="MDINSEEYKQEVLIKDVVMLAARILLESGAEGTRVEDTMTRIAK
                     KLGYSESNSFVTNTVIQFTLHSESFPRIFRITSRDTNLIKISQANKISRQITNNEISL
                     AEAKTQLEKIYVAKRDSSLPFKGFAAAMIAMSFLYLQGGRLIDVLTAILAGSLGYLVT
                     EILDRKLHAQFIPEFIGSLVIGIIAVIGHTLIPTGDLATIIIAAVMPIVPGVLITNAI
                     QDLFGGHMLMFTTKSLEALVTAFGIGAGVGSVLILV"
     gene            945..1397
                     /locus_tag="P6L01_002407"
     CDS             945..1397
                     /locus_tag="P6L01_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDP3031447.1"
                     /translation="MFWILNFIFSFLASMFFCVIFDAPRKLYLSCGFVGTCGWMVYTL
                     FFNGFNVHTIYSSFFGSLALGLLSHYMARKQKEPAIIFMVTGIIPLVPGGLAYDATKN
                     LVLLNFSTAINTMLEVTLIAGAIALGLLFADQISKLIVSGFVKSFKRL"
     gene            complement(1538..3022)
                     /locus_tag="P6L01_002408"
     CDS             complement(1538..3022)
                     /locus_tag="P6L01_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDP3031448.1"
                     /translation="MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIK
                     QSGPIAASIGIVIGALLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFL
                     TFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLLVFMLV
                     TIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVI
                     VSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLSTSHQ
                     SLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMP
                     TMFSKLHSKYKTPYVAIIFLVGVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSAA
                     KLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLIIG
                     LIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK"
BASE COUNT         1041 a          574 c          498 g          979 t
ORIGIN      
        1 atacttcaag agaattttaa ctactaaaaa ttccaatgat tattattaca atagtatcaa
       61 atattagttt tttaaaatca ataacaactt atcaaaaagc tcatgtggtt attttatagt
      121 gtataaacta taatgagtat taaattctta taaacaatgg tgatgaaatg gacataaatt
      181 cagaagaata caaacaagag gtacttatca aagacgttgt catgcttgct gctcgcatac
      241 tattagaatc tggtgcagaa ggtacgcgtg tagaagatac catgacacgt attgcaaaaa
      301 aacttggtta cagtgaaagt aacagctttg ttacaaacac tgtcatccag tttacgttac
      361 attcggaatc gtttcctaga atatttagaa ttacctctcg agatacaaac ttaataaaaa
      421 tttcgcaagc taataaaatt tcgcgtcaaa ttacaaataa tgaaatttct ttagccgaag
      481 caaaaacaca acttgaaaaa atatatgttg ctaagcgtga cagcagtctt ccctttaaag
      541 gttttgctgc agcaatgatt gcaatgagtt tcttatattt acaaggtggt agattgattg
      601 atgttttaac tgcgatatta gcaggtagtc taggatacct agtcactgag attttagatc
      661 gcaagttaca tgcacagttt atcccagaat tcattggttc attagttatt gggattatcg
      721 ccgttattgg acatacactt atcccaacag gtgacttggc aactattatc attgcggcag
      781 tcatgcctat tgttcctggt gtattaataa caaacgcaat acaagattta tttggtggac
      841 acatgttgat gttcacaacg aaatcattag aagcattggt tactgcgttt ggcatcggtg
      901 ctggcgttgg tagcgtatta attttagtat aggagtatca gactatgttt tggatcttaa
      961 actttatctt tagcttttta gcttcaatgt tcttctgcgt catttttgat gcacctagaa
     1021 aattatatct atcatgtggt ttcgttggta catgtggatg gatggtttac accttattct
     1081 tcaacggctt taatgtgcac actatatact ctagtttctt tggtagttta gcattaggct
     1141 tgttaagtca ttatatggct cgtaaacaaa aagaacctgc catcattttt atggtaacgg
     1201 gtatcattcc attagtacca ggtggattag catacgatgc tacaaaaaat ttagtcttat
     1261 taaatttcag cacagcaatc aataccatgt tagaagtaac acttattgca ggcgccatcg
     1321 cattaggttt attattcgcc gaccaaattt ccaaattaat tgtttctggg ttcgtgaaat
     1381 cttttaaacg attataaata ttgttagaaa tggctcagcc caaactctta gcaattatat
     1441 ataaggttca gctgcctcgt cttaaatgtc aatttataat acaaaaaatc gcacgatacg
     1501 cctccgaatg aagaaggtat attgtgcgat tcttttatta tttagttttt tctttttcag
     1561 gttcttcaat catatcatca acttcatttt cacttctatt taaaatcaag cgacttaatt
     1621 catcattatc catatttttc aatttaggat atcgaatcac aaagaatatt aaaccgatga
     1681 ttaaccatcc aagcaatgca atataagatg gtgcagtcag tgctgcagga gaacctggca
     1741 ctaataacaa cgctaagaaa atgaatgata caaatgagcc gataatagca aacgttttgt
     1801 aaaccggtgc atacgtatta ctttgtttgt tataactgaa taatttcgct gcagacaaac
     1861 atgtaataaa gtaggcaatg gatacacctg tagatgacat atctacaatc caagtcaatg
     1921 cagttcttcc tagccaaggt gcaattaacg acacacctac taggaatatg attgcgacat
     1981 atggtgtttt gtatttacta tgtaatttac taaacattgt tggcataata cctgaacgtc
     2041 ccatagaaaa taacaagcga cttgaactca tcaagaatcc atttaaacca gtaaatatac
     2101 ccatcataat tgcaattgct aatacaccta atccaatata accaaatgct gtttgtgtaa
     2161 cagcacctgt taaccacaac tgcccattta aactttgatg acttgttgat aaccaaccag
     2221 tgtataaaat catgacaaca taagttaatg atgctgctaa taaactgtac acgataagct
     2281 taaatgtctt gtttggtgca aagttaaact cttctgctgt ttgtggaata ttatcaaatc
     2341 caacatatgc ccatggtgcc acggatacaa taaccacaat agacactaac catcctttgc
     2401 taggttcagc taacggttgt aaattttcaa gtgcaaaatt attaccaaag aatgaaccaa
     2461 agaacatcaa taatacgacg attaccatcg ccacacagaa ataatattgt aatgatccag
     2521 atacacttgc gccacgaatc gttactagca tgaatacaag tagtaatacg gtcgcaataa
     2581 tgatttccgt aatataaacg tcccagcccg caatggtgta tagtttccca ttatttaaga
     2641 catctggcaa taagaattta actagtaaac tgaatgcggt cgcatttaaa gcaacgacac
     2701 agacataacc aaaagttaaa aaccatgatg agaagaaact cacatatctg ccgaaactta
     2761 agaaactaaa ggcaaacgcg ccccctgata ctggaaatct ctctactaat gcgccataac
     2821 taaccgcaat taatatcatt aataatgcac caataactat accaattgat gctgcaatcg
     2881 gacctgactg cttaatccag tctcctggta agatgaatgc gccccatccg atacatgaac
     2941 catatgcaat cgcccataca aacttttcag ataggttttg ttttaaatcg cctctatcta
     3001 tttgcttatt ctttttttcc ataaataaaa actcacctcg aaggtattct atacccaaaa
     3061 gatgagtttg taactctttt tcttgattat tt
//
LOCUS       DAOBBH010000221         3057 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.221, whole genome shotgun sequence.
ACCESSION   DAOBBH010000221 DAOBBH010000000
VERSION     DAOBBH010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.221"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            62..532
                     /gene="nrdR"
                     /locus_tag="P6L01_002409"
     CDS             62..532
                     /gene="nrdR"
                     /locus_tag="P6L01_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDP3031449.1"
                     /translation="MKCPKCNSTQSKVVDSRHADELNAIRRRRECENCGTRFTTFEHI
                     EVSQLIVVKKDGTREQFSREKILNGLVRSCEKRPVRYQQLEDITNKVEWQLRDEGHTE
                     VSSRDIGEHVMNLLMHVDQVSYVRFASVYKEFKDVDQLLASMQGILSENKRSDA"
     gene            533..1933
                     /locus_tag="P6L01_002410"
     CDS             533..1933
                     /locus_tag="P6L01_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HDP3031450.1"
                     /translation="MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQA
                     VGLYHFMTQFVKDSHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDE
                     QETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVT
                     RQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTK
                     DAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKL
                     QVKSPATSSSTGKSSEVNPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKTMVEEL
                     IEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQ
                     PKNEGSSGNYQKRGSYYGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDR
                     QAFLDKLSKKWEEDSR"
     gene            1933..2853
                     /gene="dnaI"
                     /locus_tag="P6L01_002411"
     CDS             1933..2853
                     /gene="dnaI"
                     /locus_tag="P6L01_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDP3031451.1"
                     /translation="MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTN
                     AMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIK
                     YDEERFEAELITSHHMQRDTLNAKLKDIYMNHRDRLDVAMAADDICTAITNGEQVKGL
                     YLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVRE
                     ANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDG
                     EEKTKAARIIERVKSLSTPYFLSGENFRNN"
     gene            2946..>3057
                     /locus_tag="P6L01_002412"
     CDS             2946..>3057
                     /locus_tag="P6L01_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031452.1"
                     /translation="MFDENMVNKLDDYYFIYGICNEYPDQDRYLKQRYLIH"
BASE COUNT         1135 a          433 c          579 g          910 t
ORIGIN      
        1 cttttttcat ctctagattt atagtataat taggcataat caaaataaag agggtgatgt
       61 aatgaaatgc ccgaaatgta attctacaca atctaaagtt gtagattcaa ggcatgccga
      121 tgaattaaat gccattcgaa gacgaagaga atgtgaaaat tgtggaacac gtttcactac
      181 atttgaacat atcgaagtta gtcagcttat agttgtgaaa aaagatggca caagagagca
      241 gttttcaaga gaaaaaatac ttaatggact tgtgcgttct tgtgagaaac gaccagttag
      301 atatcaacaa cttgaagaca taacaaacaa ggttgaatgg caattacgag atgaaggtca
      361 tacggaagtg tcttcacgag atataggtga acacgttatg aacttgttaa tgcatgttga
      421 tcaagtttca tatgttagat ttgcatcagt ctataaagaa tttaaagatg ttgatcaatt
      481 gttagcatcg atgcaaggga ttttaagcga aaacaaacgg agtgatgctt aaatgggacg
      541 acaagccttc gaattcggct taagaccaaa ggatcaattc aaagttatgc aacactttga
      601 tttaaatacg aatcatttag aggtattgaa tcgactgtat acaccgctaa ttggaacgca
      661 agcagtgggt ttatatcatt ttatgactca attcgttaaa gacagtcata atgaaacatt
      721 gattctttct cattatattt ttatgaatga acttaaaatt aatttactag agtttagaca
      781 acaaatggac ttacttgaag cgattggatt gttaaaggcg tttgtcaaac atgatgaaca
      841 agaaacacaa tttgtttacc aattaattca accgccttca gcacatttat tttttaatga
      901 tccaatgttg tcaatctttt tatatagtga agtcgagcat cgtcgatttc atgagttgaa
      961 gaaatacttt gagtatcaac aaattgattt gagtgaattt aaacaggtaa caagacaatt
     1021 tactgatgtt tttaaagtgc cgtctacaaa aatagatatc gatacaagtg atattccgat
     1081 taatgaaccg taccaaggta tcgatttatc aaatgaatct tttgactttg aaatgttgag
     1141 acaaatgtta ggtaaacatt ttattagtca agacattgtt accaaagatg caaagcgttt
     1201 aattacacaa ctcgcgactt tatatggttt aacagcagat ggcatgaagc atgttatttt
     1261 aaattcaatt acaagtggac aacagttatc ctttgaagaa atgcgtaaac aagcacgctc
     1321 gtattattta atggaacacg aaaatcaaat gcctaaatta caggtgaaat ctcctgctac
     1381 atcatcttcg acaggcaaat cttcagaagt gaatccaaag ccacaaagtg atgaatggtt
     1441 tgagttactt gaacaaacga gtccaattga tatgttagct tcttggtctg aatcagaacc
     1501 aactatatcg caaaagacaa tggttgaaga attaattgaa cgtgaaaaaa tgtcttttgg
     1561 cgtcattaat attttgttgc aatttgtcat gttaaaagaa gatatgaaac ttcctaaagc
     1621 gtatattttg gaaatagcat caaattggaa gaaaaaaggt attaagacag ctaaagaagc
     1681 atataattac gctaaaaaag ttaatcaacc taaaaatgag ggttctagcg gaaattacca
     1741 aaagcgtggt tcatactatg ggcaacgtaa tcgtatttca aaagaaaaaa cacctaaatg
     1801 gttagaaaat agagataaac ctagtgaaga agattcggct aaagataata gcgtagatga
     1861 tcaacaatta gagcaagatc gacaagcatt tctagataaa ttatctaaaa aatgggagga
     1921 ggacagtcga taatgaagca atttaaaagt ataattaaca cgtcgcagga ctttgaaaaa
     1981 agaatagaaa agataaaaaa agaagtaatc aatgacccag atgttaagca atttttggaa
     2041 gcgcatcgag ctgaattaac gaatgctatg attgatgaag acttaaatgt gttacaagag
     2101 tataaagatc aacaaaaaca ttatgacggt cataaatttg ctgattgtcc aaatttcgta
     2161 aaggggcatg tgcctgagtt atatgttgat aataaccgaa ttaaaatacg ctatttacaa
     2221 tgcccatgta aaatcaagta cgacgaagaa cgctttgaag ctgagctaat tacatctcat
     2281 catatgcaac gagatacttt aaatgccaaa ttgaaagata tttatatgaa tcatcgagac
     2341 cgtcttgatg tagctatggc agcagatgat atttgtacag caataactaa tggggaacaa
     2401 gtgaaaggcc tttaccttta tggtccattt gggacaggta aatcttttat tctaggtgca
     2461 attgcgaatc agctcaaatc taagaaggta cgttcgacaa ttatttattt accggaattt
     2521 attagaacat taaaaggtgg ctttaaagat ggttcttttg aaaagaaatt acatcgcgta
     2581 agagaagcaa acattttaat gcttgatgat attggggctg aagaagtgac tccatgggtg
     2641 agagatgagg taattggacc tttgctacac tatcgaatgg ttcatgaatt accaacattc
     2701 tttagttcta attttgacta tagtgaattg gaacatcatt tagcgatgac tcgtgatggt
     2761 gaagagaaga ctaaagcagc acgtattatt gaacgtgtca aatctttgtc aacaccatac
     2821 tttttatcag gagaaaattt cagaaacaat tgaattttaa aatgattggt gtataatgaa
     2881 tacaaatcta aatcgtttaa atgattgaag acaagatgat ctaatcaata ttacacagaa
     2941 agccattgtt tgatgagaat atggttaata aattagatga ttactacttc atttatggta
     3001 tttgtaatga atacccggat caagaccgtt atcttaagca gagatattta atacata
//
LOCUS       DAOBBH010000222         3051 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.222, whole genome shotgun sequence.
ACCESSION   DAOBBH010000222 DAOBBH010000000
VERSION     DAOBBH010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.222"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(188..823)
                     /locus_tag="P6L01_002413"
     CDS             complement(188..823)
                     /locus_tag="P6L01_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDP3031453.1"
                     /translation="MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQI
                     DSATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTY
                     NFSIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQ
                     KVADELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE"
     gene            1076..3004
                     /gene="abc-f"
                     /locus_tag="P6L01_002414"
     CDS             1076..3004
                     /gene="abc-f"
                     /locus_tag="P6L01_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HDP3031454.1"
                     /translation="MILLQLNHISKSFDGEDIFTDVDFEVKTGERIGIVGRNGAGKST
                     LMKIIAGVENYDSGNVSKIKNLKLGYLTQQMTLNSNATVFEEMSKPFEHIKRMESLIK
                     EETDWLSKHANDYDSDTYKTHMSRYESLSNQFEQLEGYQYESKIKTVLHGLNFSEEDF
                     NKPINDFSGGQKTRLSLAQMLLNEPDLLLLDEPTNHLDLETTKWLEDYLRYFKGAIVI
                     ISHDRYFLDKIVTQIYDVALGDVKRYVGNYEEFIQQRDLYYQKRMQEYESQQAEIKRL
                     ETFVEKNITRASTSGMAKSRRKILEKMERIDKPMLDAKSANIQFGFDRNTGNDVMHVK
                     NLEIGYQTAITKPISIEVSKGDHIAIIGPNGIGKSTLIKTIANQQKALNGDITFGANL
                     QIGYYDQKQAEFKSSKTILDYVWDQYPLMNEKDIRAVLGRFLFVQDDVKKIINDLSGG
                     EKARLQLALLMLQRDNVLILDEPTNHLDIDSKEMLEQALQHFEGTILFVSHDRYFINQ
                     LANKVFDLTIDGGKMYLGDYQYYIEKVEEAEALKAHQEEQSVNVQAHKKSMEQSSYHN
                     QKEQRREQRKLERQISECENEIETLETTILQIDEQLTQPEVYNNPQKANELAIQKQDS
                     EQKLEHAMSKWEELQQKL"
BASE COUNT         1147 a          482 c          476 g          946 t
ORIGIN      
        1 attaattacg attcatcatt atatctctac gtgttaattt attaacctat actatattta
       61 tcaacacaac agtttttcct ttcattttca aacataacag aaaaagcctg agacatactt
      121 agtcccagac tgttctataa ttaattaaag atataacaag gttaatcatt caaagctaac
      181 atcacattta ttcactgtaa tttttcataa agaataataa tgactgtaat tctataccta
      241 agtcaatttg atgtacttgc acatctgaag gcgtattaat tctaccagga gtgaagttta
      301 aaataccttt cacaccagct tggacgagtt catctgcaac tttctgtgca actctttctg
      361 gtgtagttag aatcacaaca tctatttctt ccttcttcaa tgttgttatt aattcatcgt
      421 tatcttttac aataacgttc cctattttct ggccaataac atcttctttt acgtcaaacg
      481 cttctgtaat cgtcatatcg tcatgtattg aaaagttata tgtgagcaaa gctttcccta
      541 ggttcccaac tccgacaatt gcgattttga tcatgtcact ctcgcttagt tcagatttaa
      601 agaaatccaa taaactatct atattatatc cgtacccttt tttacctaat tcgccaaaat
      661 atgaaaagtc acgacgaatt gttgccgagt caatttgtaa cgcatcgcta atcgcttttg
      721 aatttacacg atctatacct ttagatttta atgaactgac aaatctataa tataacggca
      781 aacgttttaa agttgctcga ggaattttaa cttggtcact cattcgctat ttcctccttc
      841 gtgtttgaat gaattatatc tatgtattca aacgagttag agcgtaattg ttagattatt
      901 aaaaaacaaa taattgatta caatgaatac ctaaatatta tacattattg aattttaaaa
      961 ataaatatct tagtttcaag acggcgtttt atactgtaaa atggttatta atgtaataat
     1021 tacgactttt ttcataagtt aataaaattt gagaaagacg aaaggtgaag atcgaatgat
     1081 acttttacaa ctcaatcata tatcaaaatc gttcgatggt gaagatatat ttactgatgt
     1141 tgattttgaa gtaaaaactg gtgaacgaat tggtatcgta ggaagaaatg gcgccggtaa
     1201 atcaacattg atgaaaatta tagctggtgt agaaaactat gattctggaa atgtgtcaaa
     1261 aattaaaaac ttaaaactag gttatttaac tcaacaaatg actttaaatt ctaatgcaac
     1321 agtttttgaa gaaatgtcaa aaccatttga acatattaaa cgaatggaaa gcttaatcaa
     1381 agaagaaaca gattggttat caaaacatgc aaatgattac gatagtgata catataaaac
     1441 acatatgtcc cgttatgaat ctttatcgaa tcaatttgaa caattagaag gatatcaata
     1501 tgaaagtaaa attaaaacag tacttcacgg gttaaatttt agtgaagaag atttcaataa
     1561 acctatcaat gattttagcg gcggccaaaa aacacgttta tctttagctc aaatgctatt
     1621 aaacgaacct gatttattac ttttagatga acctactaac cacttagatt tagaaacgac
     1681 aaagtggctt gaagattatc tacgttattt taaaggtgca atcgtcatca tcagccatga
     1741 tcgttacttt ttagataaaa tagttactca aatttatgat gtggctttag gtgatgtcaa
     1801 acgctatgtt ggtaattacg aggaatttat acagcaacgg gatttatatt atcaaaaacg
     1861 aatgcaagaa tatgaaagtc aacaagcaga aataaaacga ttagaaactt ttgttgagaa
     1921 aaatattacc cgtgcttcaa caagtggaat ggcaaaaagt agacgtaaga ttttagaaaa
     1981 aatggaacgc attgataaac caatgttaga tgccaaaagt gcaaatattc aatttggctt
     2041 tgaccggaat acaggtaatg acgtcatgca tgtaaaaaat ttagaaatcg gttatcaaac
     2101 tgcaattacc aaacctatca gtatagaggt ctctaaaggc gatcatatag caatcattgg
     2161 gccaaatggt attggaaaat cgaccttaat taaaactatt gctaatcaac aaaaagcgct
     2221 taatggcgat attactttcg gcgcaaattt acaaattggt tattatgatc aaaagcaagc
     2281 agaatttaaa tctagtaaaa cgattttaga ttatgtgtgg gatcaatatc cgttaatgaa
     2341 tgaaaaagat attcgagcag ttcttggacg tttcttattt gtacaagacg atgttaaaaa
     2401 gataattaat gatttatctg gtggtgaaaa agcacgttta caactagcac tacttatgtt
     2461 gcaacgtgac aatgtactta ttttagatga acctaccaac cacctcgata tagattcaaa
     2521 agaaatgtta gagcaagcac tccaacattt tgaaggaaca atactatttg tttcccatga
     2581 tcgttacttt attaaccaat tagcaaacaa ggtatttgat ttaacaattg atggcggaaa
     2641 gatgtattta ggagattatc aatattacat tgaaaaagtt gaggaagccg aagcgttgaa
     2701 agcgcaccaa gaagaacagt cagttaacgt acaagcacat aaaaagtcta tggaacaatc
     2761 gtcgtatcat aaccaaaaag aacaaagacg tgaacaacgc aaacttgaac gacaaatatc
     2821 cgagtgtgaa aatgaaatag aaactttaga gactactatc ttacaaatag acgagcaatt
     2881 aacccaacca gaagtgtata acaatccaca aaaagcaaac gaattagcta tccaaaaaca
     2941 agatagcgaa caaaaattag aacacgctat gtctaaatgg gaagaattac aacaaaaact
     3001 ataaaaatat acataactct aactgtaaat tcgctatagt tagagttatt t
//
LOCUS       DAOBBH010000223         2989 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.223, whole genome shotgun sequence.
ACCESSION   DAOBBH010000223 DAOBBH010000000
VERSION     DAOBBH010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.223"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            25..2730
                     /locus_tag="P6L01_002415"
     CDS             25..2730
                     /locus_tag="P6L01_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding adhesin SdrG C-terminal
                     domain-containing protein"
                     /protein_id="HDP3031455.1"
                     /translation="MIPNRLNKFSIRKYSVGTASILVGTTLIFGLSGHEAKAAEHTNG
                     ELNQSKNETTAPSENKTTEKVDSRQLKDNTQTATADQPKVTMSDSATVKETSSNMQSP
                     QNATASQSTTQTSNVTTNDKSSTTYSNETDKSNLTQAKNVSTTPKTTTIKQRALNRMA
                     VNTVAAPQQGTNVNDKVHFTNIDIAIDKGHVNKTTGNTEFWATSSDVLKLKANYTIDD
                     SVKEGDTFTFKYGQYFRPGSVRLPSQTQNLYNAQGNIIAKGIYDSKTNTTTYTFTNYV
                     DQYTNVSGSFEQVAFAKRENATTDKTAYKMEVTLGNDTYSKDVIVDYGNQKGQQLISS
                     TNYINNEDLSRNMTVYVNQPKKTYTKETFVTNLTGYKFNPDAKNFKIYEVTDQNQFVD
                     SFTPDTSKLKDVTGQFDVIYSNDNKTATVDLLNGQSSSDKQYIIQQVAYPDNSSTDNG
                     KIDYTLETQNGKSSWSNSYSNVNGSSTANGDQKKYNLGDYVWEDTNKDGKQDANEKGI
                     KGVYVILKDSNGKELDRTTTDENGKYQFTGLSNGTYSVEFSTPAGYTPTTANAGTDDA
                     VDSDGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKGVKV
                     TLQNEKGEVIGTTETDENGKYRFDNLDSGKYKVIFEKPAGLTQTGTNTTEDDKDADGG
                     EVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDAGKHTPTKPMSTVKD
                     QHKTAKALPETGSENNNSNNGTLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT         1193 a          467 c          542 g          787 t
ORIGIN      
        1 aagacagcaa caaatagaaa aggcatgata ccaaatcgat taaacaaatt ttcgataaga
       61 aagtattctg taggtactgc ttcaatttta gtagggacaa cattgatttt tgggttaagt
      121 ggtcatgaag ctaaagcggc agaacatacg aatggagaat taaatcaatc aaaaaatgaa
      181 acgacagccc caagtgagaa taaaacaact gaaaaagttg atagtcgtca actaaaagac
      241 aatacgcaaa ctgcaactgc agatcagcct aaagtgacaa tgagtgatag tgcaacagtt
      301 aaagaaacta gtagtaacat gcaatcacca caaaacgcta cagctagtca atctactaca
      361 caaactagca atgtaacaac aaatgataaa tcatcaacta catatagtaa tgaaactgat
      421 aaaagtaatt taacacaagc aaaaaacgtt tcaactacac ctaaaacaac gactattaaa
      481 caaagagctt taaatcgcat ggcagtgaat actgttgcag ctccacaaca aggaacaaat
      541 gttaatgata aagtacattt tacgaacatt gatattgcga ttgataaagg acatgttaat
      601 aaaacaacag gaaatactga attttgggca acttcaagtg atgttttaaa attaaaagcg
      661 aattacacaa tcgatgattc tgttaaagag ggcgatacat ttacttttaa atatggtcaa
      721 tatttccgtc caggttctgt aagattacct tcacaaactc aaaatttata taatgcccaa
      781 ggtaatatta ttgcaaaagg tatttacgat agtaaaacaa atacaacaac gtatactttt
      841 acgaattatg tagatcaata cacaaatgtt agcggtagct ttgaacaagt cgcatttgcg
      901 aaacgtgaaa atgcaacaac tgataaaact gcttataaaa tggaagtaac tttaggtaat
      961 gatacatata gtaaagatgt cattgtcgat tatggtaatc aaaaaggtca acaacttatt
     1021 tcgagtacaa attatattaa taatgaagat ttgtcacgta atatgactgt ttatgtaaat
     1081 caacctaaaa agacctatac aaaagaaaca tttgtaacaa atttaactgg ttataaattt
     1141 aatccagatg ctaaaaactt caaaatttac gaagtgacag atcaaaatca atttgtggat
     1201 agtttcaccc cagatacttc aaaacttaaa gatgttactg gtcaattcga tgttatttat
     1261 agtaatgata ataagacggc gacagtagat ttattgaatg gtcaatctag tagtgataaa
     1321 cagtacatca ttcaacaagt tgcttatcca gataatagtt caacagataa tgggaaaatt
     1381 gattatactt tagaaacaca aaatggaaaa agtagttggt caaacagtta ttcaaatgtg
     1441 aatggctcat caactgcaaa tggcgaccaa aagaaatata atctaggtga ctatgtatgg
     1501 gaagatacaa ataaagatgg taaacaagat gccaatgaaa aagggattaa aggtgtttat
     1561 gtcattctta aagatagtaa cggtaaagaa ttagatcgta cgacaacaga tgaaaatggt
     1621 aaatatcagt tcactggttt aagcaatgga acttatagtg tagagttttc aacaccagcc
     1681 ggttatacac cgacaactgc aaatgcaggt acagatgatg ctgtagattc tgatggacta
     1741 actacaacag gtgtcattaa agacgctgac aacatgacat tagatagtgg attctacaaa
     1801 acaccaaaat atagtttagg tgattatgtt tggtacgaca gtaataaaga tggtaaacaa
     1861 gattcgactg aaaaaggaat taaaggtgtt aaagttactt tgcaaaacga aaaaggcgaa
     1921 gtaattggta caactgaaac agatgaaaat ggtaaatacc gctttgataa tttagatagt
     1981 ggtaaataca aagttatctt tgaaaagcct gctggtttaa ctcaaacagg tacaaataca
     2041 actgaagatg ataaagatgc cgatggtggc gaagttgatg taacaattac ggatcatgat
     2101 gatttcacac ttgataatgg ctactacgaa gaagaaacat cagatagtga ctcagattcg
     2161 gacagcgatt cagactcaga tagcgactca gattcagata gtgactcaga ctcagatagc
     2221 gactcagact cagatagcga ctcagacagc gactcagact cagatagtga ttcagattcg
     2281 gacagcgact cagattcaga cagcgaatca gattcggata gcgactcaga ctcagatagc
     2341 gactcagatt cagacagcga ctcagattca gacagtgact cagactcaga cagcgactca
     2401 gattcagaca gcgattcaga ttcggatagc gactcagatt cagatagcga ctcagattca
     2461 gatagcgact cagactcaga cagcgattca gactcagata gcgactcaga cagcgattca
     2521 gattcggata gcgattcaga ttcagatgca ggtaaacata ctccgactaa accaatgagt
     2581 acggttaaag atcagcataa aacagctaaa gcattaccag aaacaggtag tgaaaataat
     2641 aattcaaata atggcacatt attcggtgga ttattcgcgg cattaggatc attattgtta
     2701 ttcggtcgtc gtaaaaaaca aaataaataa tatgattaac ttaaccaggt ccatgtggcc
     2761 tggttttttc tgtttagaaa ttcccagata taaaacaaaa aatctttaag gaatagatgc
     2821 aatttaattt gttacagaaa tgtaattgtc tatgaattag aaaagctaag atattaattt
     2881 taagggcaaa taatactatt ttttatgata attaaataaa gattacaaaa ctcataaaaa
     2941 cttttattta aacacaaatt ttaaataacg ttttacgata aagaaaaat
//
LOCUS       DAOBBH010000224         2940 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.224, whole genome shotgun sequence.
ACCESSION   DAOBBH010000224 DAOBBH010000000
VERSION     DAOBBH010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.224"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(152..901)
                     /locus_tag="P6L01_002416"
     CDS             complement(152..901)
                     /locus_tag="P6L01_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDP3031456.1"
                     /translation="MSNTNKHYIEEEYATEQSRFFKRDIGFIFLYIFLVNILPIIIVF
                     LTFGLVAVFTQQAPWGFSFPISMNISYLLAVIISLAIFRRMHEDAFVPMIRTQIIAST
                     KYKWHILISFTLSVVLFLLLNYFDIFALNVDAFTVFDIYPLIRDYGSISLIVMLWLTI
                     ILNTLICQLIFRHILIQELSRLIPLKITIVLSVILETLYYYPYINTWWEFIPALILAS
                     SATYLYLKSNRNFMVSYFYQLAVILVLHIFM"
     gene            1096..2673
                     /locus_tag="P6L01_002417"
     CDS             1096..2673
                     /locus_tag="P6L01_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HDP3031457.1"
                     /translation="MTFLTVMQFIVNIIVVGFLLTVIVIGLIWLIKDKRQSQHSVLRN
                     YPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKD
                     YQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSD
                     DHAIKLGEHLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSE
                     YHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLSNVRAFELKLAQGAKTRGGH
                     MEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVV
                     SKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGML
                     EKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCP
                     VGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRK
                     VDGELQTIHDYKLKLIS"
BASE COUNT         1009 a          404 c          564 g          963 t
ORIGIN      
        1 tgaacattga tttatgtctc aggctctcta tatggctcgt ttttagaacg acagtatatc
       61 taaataaagc acgcttaaaa gtgagttgtg atactgactt ttaagtgtgc tttttgatgt
      121 gtatggataa tggataatac tctgaaaaat tttacatgaa aatatgcaaa acgagtataa
      181 ctgctaattg atagaaatag ctcaccataa aattacggtt tgactttaaa tataagtaag
      241 tcgcactact agctagtatc aatgctggaa tgaattccca ccatgtatta atgtatggat
      301 aatagtacag tgtttcaagg ataacggata atactattgt aatctttaaa ggtattaatc
      361 tgcttaattc ttgaattaaa atatgacgga aaataagttg gcaaatcaaa gtatttaata
      421 taatggttaa ccacaacatt actattaaac tgatggaacc ataatctcta atgagcgggt
      481 aaatgtcaaa gacagtaaag gcatctacat ttagtgcgaa aatatcgaaa taatttaaaa
      541 gtaaaaagag tacgacactt agtgtaaatg atataagaat atgccattta tatttggtgc
      601 tggcaatgat ttgcgtacgt atcatcggaa caaatgcatc ttcatgcatc cgacgaaaaa
      661 tagctagtga aataataact gcgagtaaat agctaatgtt cattgaaata ggaaaagaga
      721 aaccccatgg ggcttgttga gtgaatacag ctactaaccc aaaagttaaa aagacgataa
      781 tgattggcaa gatgttaacc aaaaatatat aaaggaaaat aaatccaata tcacgtttga
      841 aaaaacgcga ttgttcagta gcgtattctt cttctatgta atgtttattt gtatttgaca
      901 tagtatacct cttaaatagt tgtattatat agatacttta gcacatatta ctttgtattg
      961 tatgttttat acattaaaat ttaaaatgaa aaacatatca taaaattgtt ttataaaatg
     1021 aagcgcttcc attgtgtttt gttttgtaag gtgtatcata aatattgaat tgaaattttg
     1081 gggggaggta ttgtaatgac gtttcttaca gtcatgcaat ttatagttaa cattatcgtt
     1141 gtaggcttcc tgcttacggt tattgttatc gggcttattt ggttaattaa agataaaaga
     1201 caatcacaac atagtgtatt aaggaattat cctttactag cacgtattag atatatttca
     1261 gaaaaaatgg gaccggaatt acgtcagtat ttattttctg gggataatga agggaaacct
     1321 ttttcacgta atgattataa aaatatcgtt ttggctggaa aatataactc tcgtatgacc
     1381 agcttcggta ctactaaaga ttatcaagac ggcttttaca tacagaacac aatgtttccg
     1441 atgcaacgta atgagatttc agtagataat acaacattgt tatcaacatt catttataaa
     1501 atcgcgaatg agcgtttatt tagtcgtgaa gaatatcgtg taccgacaaa gattgatccg
     1561 tattacttaa gtgatgacca tgcaataaaa ttaggtgaac atttaaaaca tccatttatt
     1621 ttaaaacgta tcgtaggaca atctggtatg agttatggcg ctttaggaaa aaatgccatt
     1681 acagctttat ctaaaggtct agctaaagcg ggcacttgga tgaatacagg tgaaggtggc
     1741 ttatcagaat atcatttaaa aggtaatggg gatatcattt tccaaattgg tcccggttta
     1801 tttggtgttc gtgataaaga aggtaatttt agtgaaggtt tatttaaaga ggtcgcacag
     1861 ttatctaacg tacgcgcatt tgagctgaag ttggcacaag gtgctaagac tcgtggtggt
     1921 catatggaag ctgaaaaggt aaacgaagaa attgctaaaa tccgaaatgt tgaaccttat
     1981 aaaacaatca attcacctaa ccgttacgaa tttattcata atgctgaaga tttgattcgt
     2041 ttcgtcgatc agttgcagca attaggtcaa aaaccagtag gattcaaaat tgtagtaagc
     2101 aaagtttcag aaattgaaac acttgtacgt acgatggtgg aactagataa gtatccaagc
     2161 tttattacga ttgatggtgg tgaaggtggt actggtgcaa cattccaaga attacaagat
     2221 ggtgttggct taccgctatt tacagctcta cctattgtgt ctggcatgtt agaaaaatat
     2281 ggtattcgag ataaagtgaa attggcggca tctggtaagt tagtgacacc agataaaatt
     2341 gcgattgcac taggtttagg tgcagatttt gtaaatatcg cacgtggcat gatgattagt
     2401 gtcggttgta ttatgagtca acaatgccat atgaatacat gtcctgtagg tgttgcaacg
     2461 acagatgcga aaaaagaaaa agcattgatt gttggagaaa agcaatatcg tgtcacaaac
     2521 tatgtaacaa gtttgcatga aggcttattc aatattgcag cagctgttgg cgtatccagt
     2581 cctacagaaa ttactgctga tcatattgta tatcgaaaag tcgatggtga gttacaaacg
     2641 atacatgatt ataaattgaa attgattagt tgatttatct atttcgagaa aatgaaagca
     2701 gcggatttta gtgttactgc aaataatttt atattagtag tggatgctgg tcacacaaga
     2761 atttcaaata ttaaagccct cagaatatga attaaggttt gtatccttag tcttatctga
     2821 gggcattttt atgttataaa ctatttgtcg tccattttat ctttttcttt taaacctctg
     2881 tgctttaatt gcttttcaag tttttcaaaa ctaatatctt tattttcttt agttgaaaca
//
LOCUS       DAOBBH010000225         2900 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.225, whole genome shotgun sequence.
ACCESSION   DAOBBH010000225 DAOBBH010000000
VERSION     DAOBBH010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.225"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            163..1473
                     /gene="der"
                     /locus_tag="P6L01_002418"
     CDS             163..1473
                     /gene="der"
                     /locus_tag="P6L01_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019167598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDP3031458.1"
                     /translation="MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYS
                     SGEWLTHDFNIIDTGGIEIGDAPFQTQIRAQAEIAIDEADVIIFMVNVREGLTQSDEM
                     VAQILYKSKKPVVLAVNKVDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVV
                     SHFGEEEEDPYDEDTIRLSIIGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEY
                     SYDGQDYVLIDTAGMRKKGKVYESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDK
                     RVAGYAHEQGKAVVIVVNKWDTVEKDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERT
                     RLRTLFPYINEASENHKKRVQSSTLNEVVTDAISMNPTPTDKGRRLNVFYATQVAIEP
                     PTFVVFVNDVELMHFSYKRYLENQIRAAFGFEGTPIHIIARKRN"
     gene            1490..2488
                     /locus_tag="P6L01_002419"
     CDS             1490..2488
                     /locus_tag="P6L01_002419"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDP3031459.1"
                     /translation="MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCH
                     TNKKYLKYAKLDVNIIATSDMTKAIQFADIYLMALPTKAMREVATQINDKLTSKKTFI
                     HVAKGIENGTFKRVSEMIEDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKS
                     VSKLTQDLFMNDYLRVYTNDDLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTR
                     GLAEISRLGEKLGADPMTFLGLGGIGDLIVTCISTHSRNFTLGYKLGQGESMDQALSE
                     MNMVVEGIYTTKSVYHLAKEKNVDMPITNALYRVLFENISVKECVKDLMERDKKSE"
     gene            2659..>2900
                     /locus_tag="P6L01_002420"
     CDS             2659..>2900
                     /locus_tag="P6L01_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDP3031460.1"
                     /translation="MNKTDLINAVAEQADLTKKEAGSAVDAVFESIQNSLAKGEKVQL
                     IGFGNFEVRERAARKGRNPQTGKEIDIPASKVPAFKA"
BASE COUNT         1026 a          401 c          595 g          878 t
ORIGIN      
        1 acaaaatata ctcggaatga taacgtggac aaatttaact aagtgtttaa aaagataaag
       61 ttttaagtgc tgatttttat cattacagta ataaactcat tttgaataca cagtctcatg
      121 tgatattatt aaaaagatat aagaaagaga ggaagttagc ttatgactaa acctatagta
      181 gctattgtag gtaggcctaa tgtaggtaaa tctacaattt ttaatagaat agttggagaa
      241 cgtgtttcga ttgtggaaga cacgccaggt gtaacacgag atcgtattta ttcttcaggt
      301 gaatggttaa cacatgattt caatattatt gatacaggtg gtattgaaat tggtgatgca
      361 ccattccaaa cacaaattag agcgcaggca gaaatcgcca tagatgaagc ggatgttatt
      421 atttttatgg ttaacgtgcg tgaaggattg acacaaagcg atgaaatggt cgctcaaatt
      481 ttatacaaat ctaaaaaacc ggtcgtatta gcggttaaca aagtagataa tatggaaatg
      541 cgtacagacg tgtatgattt ctattcatta ggatttggtg aaccgtatcc gatatcaggg
      601 tcacatggtt taggtcttgg tgacttgtta gatgcagttg tttctcattt tggtgaagag
      661 gaagaagatc cttatgatga agatacaatt cgactatcca ttattggacg accaaacgta
      721 ggtaaatcaa gtttagtaaa tgctatttta ggtgaagatc gcgttatcgt ttctaatgtt
      781 gcagggacaa cgagagacgc tattgataca gagtatagtt atgatggaca agattatgtt
      841 ttaatcgata ctgctggtat gcgtaaaaaa ggaaaagtat atgaatcaac tgagaaatat
      901 tcagtactaa gagctttaaa agcgattgaa cgttcaaatg ttgttttagt ggtcattgat
      961 gcagaacaag gcatcattga acaagataaa cgtgttgcag gatatgcaca tgaacaaggt
     1021 aaagcagtcg tgattgtcgt aaataaatgg gatactgtgg aaaaagatag taaaacgatg
     1081 aagaaatttg aagatgaagt acgtaaagaa ttccaatttt tagattatgc acaaattgct
     1141 tttgtgtctg ctaaagaacg cacaagatta cgtacattat tcccttacat taatgaagca
     1201 agtgaaaacc ataaaaaacg tgttcaaagt tcaactttaa atgaagttgt tactgatgca
     1261 atttccatga accctacacc aacagacaaa ggtagacgtt tgaatgtctt ttatgcaaca
     1321 caagttgcta tagaaccacc gacatttgtt gtatttgtta atgatgtaga attaatgcat
     1381 ttttcttata aacgctattt agagaatcaa atccgtgccg cttttggttt tgaaggtaca
     1441 ccaattcata ttatagctcg aaagagaaat taacgattgg gggataacaa tgactaaaat
     1501 taccgttttt ggtatgggaa gttttgggac agcccttgcc aatgttcttg cagaaaatgg
     1561 acatgatgtt ttgatgtggg gtaaaaatca agatgctgtt gatgaattaa atacatgtca
     1621 tacaaataaa aagtatttaa aatacgcgaa attagatgtt aacatcatcg ctacttcaga
     1681 tatgaccaaa gcaattcaat ttgcagatat ttacttaatg gctttaccta ctaaagcaat
     1741 gcgagaagtt gctactcaaa ttaatgataa gctgacctct aaaaagactt ttatacatgt
     1801 tgctaaaggt attgaaaatg ggacgtttaa acgtgtgtca gaaatgattg aagattctat
     1861 ttcacccgaa tataatgcag gtattggcgt gttgtcaggg ccaagtcatg cggaagaagt
     1921 tgtagtcaag caaccaacta cagttgctgc atcatcaaaa gataaaagtg taagtaaatt
     1981 aacgcaagat ttatttatga atgattattt gcgtgtgtac acgaatgatg acttgattgg
     2041 tgttgaactt ggtggtgcat tgaaaaatat catcgcagta gcaagtggta tcgtagctgg
     2101 aattggctac ggtgataatg caaaagctgc gttaatgact cgtggtttag cggaaattag
     2161 tagattaggt gaaaagttag gtgccgatcc tatgacattt ctaggtttag gtggtatcgg
     2221 tgacttaatc gttacttgca tatcaacaca ttctcgaaat ttcacattag gatataaact
     2281 tggacaaggt gaatcaatgg atcaagcatt atctgaaatg aacatggttg ttgaaggtat
     2341 ttatacaact aaatcagttt atcatttagc taaagaaaaa aatgtggata tgccaattac
     2401 aaatgcatta tatagagtat tatttgaaaa tatctcagta aaagaatgcg taaaagattt
     2461 aatggagcgc gataaaaaat ctgaataaaa tgtgtattat tacacatata cggctaataa
     2521 ttacgtaaaa tgaatagaaa tggcttaaat tcaacgtttt tgccacataa atgattgcga
     2581 tttatgtaat gcttgtgtta aagttttacc ataatgatat aaacatatca ttatcaaacc
     2641 tcaggaggtg aatgtctaat gaacaaaaca gatttaatca atgcagttgc agagcaagct
     2701 gatttaacta aaaaagaagc tggttcagca gtagatgctg tattcgaatc aatccaaaac
     2761 tcacttgcta aaggtgaaaa agtacaatta attggtttcg gtaactttga ggtacgtgaa
     2821 cgtgctgcac gtaaaggtcg taaccctcaa actggtaaag aaattgatat cccagcaagt
     2881 aaagttccag cattcaaagc
//
LOCUS       DAOBBH010000226         2825 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.226, whole genome shotgun sequence.
ACCESSION   DAOBBH010000226 DAOBBH010000000
VERSION     DAOBBH010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.226"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            45..1706
                     /locus_tag="P6L01_002421"
     CDS             45..1706
                     /locus_tag="P6L01_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDP3031461.1"
                     /translation="MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAP
                     FTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGF
                     LVLYFLAPYISELTLARNIHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYK
                     SMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLW
                     YYWRKRKHNIDRMVESDYTDIDVSYGKMYKEIIAYSIPFVIVSLNYPLFNLVDQFTHN
                     GALSLVGIPSQLQDIFFNMLNMSTNKIVMIPTSLSAGFAVSLIPYITKTFAEGRLHEM
                     HHQIRTSIGVLMFITVPASIGIMALAQPLFTVFYGYDPIVLGHDPNHDGSRLLFYYAP
                     VAILISLLSVTASMLQGIDKQKLTVYVILASVVIKLALNYPLIMLFHTPGAILSTSIA
                     LLFAIGCNFYILKKYAKFKFSYSWIHFAKIFLYSFIMMLGVELVFFLTNLFLEPTKLG
                     YLIIIILGVTVGILIYGTITIKTRLADEFLGEIPEKLRRRVRFLR"
     gene            1703..2398
                     /locus_tag="P6L01_002422"
     CDS             1703..2398
                     /locus_tag="P6L01_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDP3031462.1"
                     /translation="MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPE
                     NDKITVRGELIEYIENVYIMLNKPKGYISATEDHHSKTVIDLIPEYQHLNIFPVGRLD
                     KDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEDDIQAFKEGVTLTDGKVK
                     PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYR
                     LLTENDFDKLNYK"
     gene            2415..>2825
                     /locus_tag="P6L01_002423"
     CDS             2415..>2825
                     /locus_tag="P6L01_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDP3031463.1"
                     /translation="MAKGNLFKAILGIGGAVAAVLVTRKDSRDKLKAEYNKYKQDPQS
                     YKDNAKDKATQLGTIANETIKEVKTNPKEYANRLKNNPKAFFEEEKSKFTEYDNKTDE
                     SIEKGKFDDEGGAAPNNNLRIVTEEDLKKNKNALS"
BASE COUNT         1010 a          387 c          496 g          932 t
ORIGIN      
        1 ttagtttaca aaactttaat aaacatgaga tagggagatt cgtaatgagt gaaagtaaag
       61 aaatggtgcg tggaaccttt ttaattacca ttagtatatt aattaccaag gtattgggcg
      121 tactttttat cattccattc aactatttaa ttggtggaca agaaaatatg gcgccgttca
      181 catacgctta tgcaccatat aatattgcaa ttgcagttgc gacagcaggt gtaccattag
      241 cagcttcgaa atatgttgcg aaatataatg ctattggggc atataaagta agtcagaaat
      301 tttataaatc gagttttatt gtaatgagta ttaccggtgt tttaggtttc ttggtactgt
      361 atttcttagc accttatatt tctgaattaa cattagctag aaacattcat gataaaaacg
      421 gttggtcagt cgatgatatt acttggatta taagaatcat tagtatggtt gttatcttta
      481 ttcctgtatt agcgacatgg agagggatat tccaaggtta taaatctatg gggccaacag
      541 cagtgtcaga agtaactgag caaattgcac gcgtgatttt catattaatc ggaagttatt
      601 tagtactaaa tgtctttgat ggctctattt tattagccaa tggtattgct acttttgcag
      661 cagcagttgg tgcgattatt ggtatattca cactctggta ttactggaga aagcgtaagc
      721 ataatattga tcgtatggtc gaatctgatt acacagatat agatgtgtca tatggcaaga
      781 tgtataaaga aattattgct tatagtatac cgttcgttat tgtaagtttg aattatccat
      841 tatttaactt agtagatcaa tttacacata acggtgcttt atctttagtt ggtatacctt
      901 cacaattaca agatatattc tttaatatgc taaatatgtc aactaataaa attgtaatga
      961 ttccgacatc attaagtgct ggttttgcag tgagtttaat tccttatatt acaaaaacat
     1021 ttgcagaagg tcgcttacat gaaatgcatc atcaaattag aacatctatt ggtgtattaa
     1081 tgtttataac tgttccagct agtattggaa ttatggcctt ggcacaacca ttattcacag
     1141 ttttctatgg ttacgatcca attgttttag gtcatgaccc taaccatgat ggtagtcgct
     1201 tattattcta ttatgcgcct gtagcaatac taatttcact attaagtgta acggcatcaa
     1261 tgttacaagg tatagataaa cagaaattaa cagtttatgt tattttagct tctgtagtta
     1321 tcaagttagc gttaaactat ccattaatta tgttattcca tacacctggt gcaattttaa
     1381 gtactagtat cgcattatta tttgcaattg gatgtaattt ctatattctt aaaaaatatg
     1441 cgaagtttaa attcagctat agttggattc attttgcaaa aatattcttg tattcattta
     1501 ttatgatgct aggtgtagaa ttagtatttt tccttacaaa tctattctta gaacctacta
     1561 aacttggtta cttaatcatt attattttag gtgttacagt aggtattcta atttacggta
     1621 caatcacaat taaaacgcgt cttgcggacg aattcttagg tgaaattcct gaaaaattaa
     1681 gacgtagagt taggttttta cgatgagaat agataaattt ttagcaaata tgggcgtcgg
     1741 tacacgcaat gaggtcaaac agttacttaa aaaaggcctt gtaaatgtca acgaacaagt
     1801 aattaaatca ccaaaaacac atatagaacc agaaaacgat aagataacag ttcgtggaga
     1861 attaatagaa tacattgaaa atgtctatat tatgttaaat aagccaaaag gttatatatc
     1921 agcaactgaa gatcatcatt caaaaaccgt tattgattta atacctgaat accaacatct
     1981 aaatattttt ccggttggtc gtcttgataa agatacagaa gggcttttat tgataacgaa
     2041 tgatggtgat tttaatcatg aattaatgag tccaaacaaa catgtttcta aaaaatatga
     2101 agttatttca gcaaatccta tcactgaaga cgacattcaa gcttttaaag agggtgtaac
     2161 actgactgat ggcaaggtta agcctgcaat attaacttat attgataatc aaacgtcaca
     2221 cgtaacaatt tatgaaggaa aatatcatca agttaaacga atgtttcata gcattcaaaa
     2281 tgaggtatta catttaagac gtatcaaaat tgctgattta gaattggaca gcaatttaga
     2341 ttcaggagaa tatcgtttat taactgaaaa tgattttgac aaattaaatt ataaataaaa
     2401 gggagacgat tgatatggca aaaggtaatt tatttaaagc gattttaggt ataggtggcg
     2461 ctgtagcagc tgtacttgtt acacgtaaag atagtcgtga caagctgaaa gcagaatata
     2521 ataaatataa acaagatcct caaagctata aagataatgc taaggataaa gcgacgcaat
     2581 taggaacaat tgctaatgaa acaattaaag aagtaaaaac aaatccgaaa gaatatgcta
     2641 atagattaaa aaataatcca aaagcatttt tcgaagaaga aaaatcaaaa tttaccgaat
     2701 atgacaataa gactgacgaa agtattgaaa aaggtaaatt tgatgatgaa ggtggcgcag
     2761 caccaaataa taatttacgt atcgtcactg aagaagattt aaaaaagaat aaaaatgcat
     2821 tatct
//
LOCUS       DAOBBH010000227         2820 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.227, whole genome shotgun sequence.
ACCESSION   DAOBBH010000227 DAOBBH010000000
VERSION     DAOBBH010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.227"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..257
                     /locus_tag="P6L01_002424"
     CDS             <1..257
                     /locus_tag="P6L01_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDP3031464.1"
                     /translation="ALKKMFVDKGYRNLKIGKKLLDKVIMTCKEQNIDGIYLGTIDKF
                     ISAQYFYSNNGFREIKRGDLPSSFPKLDVDNRFYYRNLKD"
     gene            770..2581
                     /locus_tag="P6L01_002425"
     CDS             770..2581
                     /locus_tag="P6L01_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDP3031465.1"
                     /translation="MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSG
                     GFLGVDTFFVISGYLITSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFT
                     LIFKPELIIQMKRDAIAAIFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLL
                     FPLVITFLLHRFKPRNIIQTLFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLL
                     GCILAFIWPPFALKKDISKKIVVSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYII
                     SFATLFIIAIAVHPSSLFAKFLSMKPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIP
                     VYVYIIEILLTALMAEISYRFIETPIRKKGFKAFAFLPKKKGQFARTVLVILLLVPSI
                     VVLSGQFDALGKQHEAEKKEKKTEFKTTKKKVVKKDKQEDKQTANSKEDIKKSSPLLI
                     GDSVMVDIGNVFTKKIPNAQIDGKVGRQLVDATPIVKSQYKDYAKKGQKVVVELGTNG
                     AFTKDQLNELLDSFGKADIYLVSIRVPRDYEGRINKLIYEAAEKRSNVHLVDWYKASA
                     GHPEYFAYDGIHLEYAGSKALTDLIVKTMETHATNKK"
BASE COUNT          991 a          361 c          497 g          971 t
ORIGIN      
        1 ctgcacttaa aaaaatgttt gttgataaag gttataggaa tttaaaaata ggtaaaaaac
       61 ttttagataa agttataatg acatgtaaag aacaaaatat cgatggtatt tatttaggaa
      121 caattgataa atttattagt gcacagtatt tttattctaa taatggcttc cgtgaaatta
      181 agcgaggaga tttaccaagt agttttccta aattagatgt agataatagg ttttattaca
      241 gaaatttaaa agattaattc gatgcaatga ataattggta tgacaatctt agtaatattc
      301 attttaaaag tgacacatag cgtttaaata taaaggctga cgtgatatat cgcttggttt
      361 aaattagagg ataggctgca tttcgcttta ttttcaatac atggtaattt attcattata
      421 gaaattttga aaaagtaaat tcgaatagca tatcttaatg attctgttac agtaagatgc
      481 ataaaatata aaacgaacct ttttttgtaa aaatataatg aaaataacag ttattttgct
      541 aaaaattcaa gaatctatga taatctatgt tatgtagtaa aatcgacaaa tttatatgtg
      601 gtttgcagta atgaaattaa ctgtaataat tgttttctac ttaataaaaa atttaaaact
      661 atcttaatta agggttaaat caattaatga aaatcagtgc aatattttat tttcaaaaat
      721 attgtgatat gctacaaaat agagaaaaat aaatggggcg tttattaaca tggatacaaa
      781 agactttaaa cgtttagaaa aaatgtattc cccgcgatac ttacccggat tagatggatt
      841 gagggcattc gcagttatag gaatcattat ttatcacttg aatgcacaat ggttaagtgg
      901 gggcttttta ggagtagata cattcttcgt tatttcaggt tatttaataa caagtttgtt
      961 gataagtgag tattatcgga cgcaaaaaat cgatttgcta gagttttgga agcgacgatt
     1021 gaaacgactc attccggcag tgttattttt aatctgtgtc gtgcttacgt tcacactgat
     1081 atttaaaccg gaattgatta tacaaatgaa acgagatgct attgcagcta tattctatgt
     1141 ttcaaactgg tggtacattt cacagaatgt agattatttt aaccaatttg ctattgaacc
     1201 actcaaacat ttatggtctt tagccattga agaacaattt tacttgcttt tcccattggt
     1261 tatcacgttc ttattacata gatttaaacc gagaaatatt attcaaacgc tatttattgt
     1321 atcgttgatt tctttaggac ttatgatagt gattcatttc atcactggag ataattcacg
     1381 tgtgtatttt gggacagata cacgactgca aactttattg cttggttgta tattagcatt
     1441 tatttggcct ccgtttgctt tgaaaaaaga tatttctaaa aagattgtcg tatcattaga
     1501 tattataggg atatctggtt ttgcggttct aatgactttg ttctttatag ttggcgacca
     1561 agatcaatgg atctataatg gaggatttta cattatatca tttgcaactt tattcattat
     1621 tgcaattgcg gtacatcctt ctagtttatt tgctaaattt ttaagtatga aacctttact
     1681 aattataggt aaacgatcat atagcttata tttatggcat tatcctatca ttgtttttgt
     1741 gaacagttat tacgtacaag gacaaatacc ggtatacgtt tatattatag aaattttgtt
     1801 aacagcgtta atggctgaaa tttcgtatcg ctttattgaa acacctatac gtaaaaaagg
     1861 atttaaagct tttgcatttt tacctaaaaa gaaggggcaa tttgctagaa cagtgttagt
     1921 tatcctatta ttggttccgt ctatcgttgt gctcagtgga cagtttgatg cacttggcaa
     1981 acaacatgaa gccgagaaga aagagaagaa gacggaattt aaaacaacga agaaaaaagt
     2041 cgttaaaaaa gataagcaag aggataagca gacagcgaat agcaaagagg atattaaaaa
     2101 gtcatcacca ctattaattg gtgactcggt catggtggat attggtaatg tctttactaa
     2161 gaaaatacca aatgcacaaa ttgatggtaa agttggacgg caactcgttg atgctacacc
     2221 aattgtgaaa tcgcaatata aagactatgc taaaaaaggt caaaaagttg tagtagagct
     2281 tggtacaaat ggggcattta cgaaagatca attaaatgaa ctattggata gttttggaaa
     2341 agcagacata tatttagttt ctattagagt acctagagat tatgaaggta gaataaataa
     2401 attaatttat gaggcagctg aaaagcgctc taatgtacat ctagtcgatt ggtataaagc
     2461 ttctgcaggt catccggaat actttgcata tgacggtatt cacttagaat atgcaggtag
     2521 taaagcgctg actgatttga ttgtaaaaac gatggaaaca catgctacaa ataagaaata
     2581 acttgatgca ctaaactttt gaaatattac attacttctg atatttatta tcaaaaatga
     2641 tgtatttcat taaaagttta gtgctttttt attttcaaat cccatgttaa cggtgcagaa
     2701 aaagtgttgt aaacattcta attggtatat tacattcaat gaagctttat taggaacaga
     2761 ttacattatg ataacaaagc ccgcaagaca cctaatctct gttatagttt gttttgtcgc
//
LOCUS       DAOBBH010000228         2779 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.228, whole genome shotgun sequence.
ACCESSION   DAOBBH010000228 DAOBBH010000000
VERSION     DAOBBH010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.228"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            85..879
                     /locus_tag="P6L01_002426"
     CDS             85..879
                     /locus_tag="P6L01_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDP3031466.1"
                     /translation="MIHSKKLTLGICLVLLIILIGGCIIMTKINSRNAQIKDTFNQTL
                     NVYPTKNLDDFYDKEGFRDQEFDKRDKGTWIINSGMYIQLKGGALKSRAMVLYINRNT
                     RTAKGYFLISETTEDKKGYVHNKDKKYPVKMERNRIIPTKPITDEKLKKEIENFKFFV
                     QYGNFKDFKDYKDGDISYNPNVPSYSAKYQLNNDDYNVQQLRKRYDISTKRAPELKLR
                     GSGDLKGSSVGSKELEFNFVRNKEENVYFSDGINFKPTEEMNHEQN"
     gene            866..2191
                     /locus_tag="P6L01_002427"
     CDS             866..2191
                     /locus_tag="P6L01_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031467.1"
                     /translation="MNKTKYQINSDNIKSNSEETSAISSISYEIENANNNDLNNASIQ
                     TQIEILKSQNSFPKNLSYLKSYTDPKTGTTTSAFLNKDTGKVTLGMTGTNVHKDAILK
                     QTFGVPSYQGYIDASETLKDIGADVNIGLHSVTDKDPHYKNTQDFIKNIKKDYDIDII
                     TGHSLGGRDAMILGMSNDIKHIVVYNPAPLAIKDVSGLYADQEELKKLIEKYDGHIVR
                     FVSDEDELDAGVRNHLYETAGEKIVLKNGEGHAMSGILMSRTQAIILAELNKVKGYQD
                     ENNKALKSVRKQTRHRLHKVETLRANWIQTTGGSLSSSQQQLLEALTALTIAEGLNQL
                     VNEESQHLKKMYHAMAHKFGDNWKKAQEVGNGIGEKLTSEEVIDELRKGGAYESKLET
                     DPKRKIDDKIKKLNDVYKNCNGYIAKIKQSIEAIVSNDQMLASQIDGMM"
     gene            2191..2517
                     /locus_tag="P6L01_002428"
     CDS             2191..2517
                     /locus_tag="P6L01_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031468.1"
                     /translation="MFTTYKNINELENAYDEERKQLNDAFNQIDELRHQTRKKCEQMY
                     DHFLYLKHKMNYSEDAMIRMTRIIESFDRETNQRIRHHEMKLEDYKDELRREYLKQSD
                     RIEGDE"
     gene            2520..>2779
                     /locus_tag="P6L01_002429"
     CDS             2520..>2779
                     /locus_tag="P6L01_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031469.1"
                     /translation="MGLDFSGLPDLAVLEQMKEKEQISEVIAPEHVRMHHDHQNKLKS
                     DEKILLDQMVSHFKNFEDDFKNAAQGAWVKNATDELKDISND"
BASE COUNT         1141 a          374 c          515 g          749 t
ORIGIN      
        1 aattcatttg ttcaaaacaa tttttcgctt tagttgtaaa tattaaaaac tgagtcagaa
       61 ttataaatat aaggtggaga aatgatgatt cattcaaaaa aattaacgct cggtatatgt
      121 ttggtattac tcattatatt gattggaggt tgtatcatta tgacaaaaat aaatagtcga
      181 aatgcccaaa ttaaagacac atttaatcaa acattaaatg tgtatccaac caagaatctt
      241 gatgattttt acgataagga aggctttcga gatcaagaat ttgataaaag agataaaggg
      301 acttggatta ttaattctgg aatgtacatc caattaaaag gaggagcctt aaaatccaga
      361 gcgatggtac tttacatcaa tcgtaacact agaacagcta agggatattt tttaataagt
      421 gagacaacag aagacaaaaa aggatacgtt cataataaag ataaaaaata ccctgtcaag
      481 atggaacgta atcgaataat tccaacgaag cccataacgg acgagaagtt aaaaaaagag
      541 attgaaaact ttaagttctt tgtacaatat gggaatttta aagattttaa agattataaa
      601 gatggggata tttcatacaa tcctaacgta ccaagttatt ccgcaaagta tcaattgaat
      661 aatgatgatt ataatgtcca acaattaaga aaacgatatg atatatcaac caaaagggca
      721 cctgaattaa aattgagagg atctggcgat ttaaaaggct catccgtagg ttctaaagaa
      781 ctagaattta actttgtaag aaataaagag gaaaatgtgt atttttctga tggtattaat
      841 ttcaaaccta ctgaggaaat gaatcatgaa caaaactaaa tatcaaataa attctgacaa
      901 tataaagagt aactctgaag aaacaagcgc aatatctagt ataagttatg aaatagaaaa
      961 cgcaaataat aacgatttaa ataatgcaag tattcaaacg caaatagaaa tattaaaatc
     1021 acaaaatagt ttccctaaaa atctttcata tcttaaaagt tatacggacc ccaaaacagg
     1081 tacgactaca agcgcctttt taaataaaga cactggcaaa gttactttag gtatgacagg
     1141 tactaatgta cacaaagacg caatattaaa acaaacattt ggtgttcctt cttatcaagg
     1201 atatatagat gcgagtgaaa cgctaaagga tattggggcc gatgtcaata ttggccttca
     1261 ttccgtcaca gataaagatc cacattataa aaatacccaa gactttatca aaaatatcaa
     1321 aaaagactat gatattgata ttattaccgg acattcgctg ggcggtagag atgcgatgat
     1381 tttaggtatg agtaatgata ttaaacatat cgttgtgtat aatccagctc cattagcgat
     1441 caaagatgtg agcggccttt atgccgatca agaagaactt aaaaaattga ttgaaaagta
     1501 cgatggtcac attgtaagat ttgtgtctga tgaagacgaa ttagatgcag gtgtccgcaa
     1561 tcatttatat gaaactgctg gagaaaaaat agtacttaaa aatggagaag gccatgcaat
     1621 gagtggtatt ttaatgagca gaacacaggc tataatctta gctgaattaa acaaagttaa
     1681 aggctaccaa gacgaaaata ataaagcatt aaaatccgtt cgtaaacaaa cgaggcatag
     1741 attacataaa gtagagacgt taagagcgaa ttggattcaa acaacgggtg gatcactctc
     1801 ttcctcccaa caacaattat tagaagcttt aacagcacta accattgccg aaggcttaaa
     1861 tcaattagtg aatgaagaaa gccaacattt gaaaaaaatg tatcacgcga tggcacataa
     1921 atttggagac aactggaaaa aagcgcaaga agttggaaat ggaattggtg aaaaattaac
     1981 ctctgaagag gttatagatg aattaagaaa aggtggcgcg tatgaaagta aacttgaaac
     2041 agatcccaaa agaaaaattg atgataagat aaagaaatta aatgatgttt ataaaaattg
     2101 taatggctat atcgcaaaaa ttaaacagag tatcgaagca attgtttcta atgaccaaat
     2161 gttagcgagc cagattgatg ggatgatgta atgtttacta cgtataaaaa tattaatgaa
     2221 cttgaaaatg cctatgatga agaaagaaaa caattgaatg atgcattcaa tcaaattgat
     2281 gaattaagac atcaaacacg caagaaatgt gaacaaatgt atgatcattt cttatatctc
     2341 aaacataaaa tgaattatag tgaagacgct atgatcagga tgacacgtat tatagaatct
     2401 ttcgatagag aaacgaatca acgtatccga catcacgaaa tgaaattaga agattataaa
     2461 gatgagttaa gaagagaata tctaaaacaa tctgacagaa ttgaaggaga tgaataagcg
     2521 tgggacttga ttttagtggt ttaccagatt tagcagtatt ggaacaaatg aaggaaaaag
     2581 aacagattag tgaggttatt gcgcctgaac atgttcgtat gcatcatgat catcaaaata
     2641 agctgaaaag tgatgagaaa atattacttg accaaatggt aagtcatttc aaaaactttg
     2701 aagatgattt taaaaatgcg gcacaagggg cttgggtgaa aaatgccaca gacgaattaa
     2761 aagatattag taatgattt
//
LOCUS       DAOBBH010000229         2767 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.229, whole genome shotgun sequence.
ACCESSION   DAOBBH010000229 DAOBBH010000000
VERSION     DAOBBH010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.229"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..430)
                     /gene="deoC"
                     /locus_tag="P6L01_002430"
     CDS             complement(<1..430)
                     /gene="deoC"
                     /locus_tag="P6L01_002430"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDP3031470.1"
                     /translation="MNSAKLIDHTLLKPESTRTQIDQIIDEAKAYHFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVVKAAKGHTVKVIIETVLLDHDEIVKASELTK"
     gene            744..1454
                     /gene="deoD"
                     /locus_tag="P6L01_002431"
     CDS             744..1454
                     /gene="deoD"
                     /locus_tag="P6L01_002431"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDP3031471.1"
                     /translation="MTKGTPHIQPNGVKIAKTVLMPGDPLRAKYIADNFLENVEQFND
                     VRNMFGYTGTYKGKEVSVMGSGMGIPSIGIYSYELYNFFDVDTIIRIGSCGALQENVN
                     LYDVIIAQAASTNSNYVDQYNIPGHFAPIADFELVTKAKNVADQIGATTHVGNVLSSD
                     TFYNADPTFNDAWKKMGILGIEMESAGLYLNAIHAGKKALGIFTVSDHILRDEATTPE
                     ERQNSFTQMMEIALEIAE"
     gene            complement(1575..2018)
                     /locus_tag="P6L01_002432"
     CDS             complement(1575..2018)
                     /locus_tag="P6L01_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDP3031472.1"
                     /translation="MSNQQDVVKELNQQVANWTVAYTKLHNFHWYVKGPNFFSLHVKF
                     EELYNEASQYVDELAERILAVGGNPVGTLTECLEQSIVKEAAKGYSAEQMVEELSQDF
                     TNISKQLENAIEIAGNAGDDVSEDMFIGMQTSVDKHNWMFKSYLS"
     gene            complement(2218..2631)
                     /locus_tag="P6L01_002433"
     CDS             complement(2218..2631)
                     /locus_tag="P6L01_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HDP3031473.1"
                     /translation="MPIVYYDGNCIYCYNYVIWLIQHDLPRNYQFATLKGEVGQQFFK
                     QHPEAANKNSVILQKGDRIYFESQAIIKLITALPNRTKLLGVGLWLVPKPVRNFGYRM
                     FANNRNRMWKTTWHQPNDYEKSFFLDDNAKVKLTD"
BASE COUNT          852 a          484 c          412 g         1019 t
ORIGIN      
        1 cttttgttaa ttcactcgct tttacgattt cgtcatggtc caacaatacc gtctcaataa
       61 tcacttttac tgtgtgacct ttcgcagctt tcaccactgc ttcaatgtct tgttgtacat
      121 catcaaaacg tccatctttt aatgcgccga tgttgatgac catgtcaatt tcatctgcac
      181 cattttgaat cgcatcttct gtttcaaatg ctttcgtcgc agttgtcgat gcacctaatg
      241 ggaatcctat taccgtacaa actaacacct ctgaatcagc tagtcgctct gctgcatatt
      301 taacatgcgt tggattcaca catacagatt taaaatggta tgctttcgct tcatcgatga
      361 tttgatcgat ttgcgtacgt gttgactcag gcttcaataa agtgtgatca atcaattttg
      421 cactattcat ttctatctcc tcctttatgg ttgattataa aaatacggtt gtaaattagt
      481 tgattgagcg tcaggttcat ttaaatatca ggttagatgt tcgcttttta tgtaaccgca
      541 tacatatact attacattaa ttcatttccc ataaacaaac aatacaattg aacgtgatat
      601 cttcattatg aacgatgact tgacaacaag ctaatcagga ttatattttt ataattcttt
      661 aattctatag tacaaaaatt cgcaaaaaag ggaaacaaat gttatcttaa aattattaat
      721 gaatattaag gagaagataa caaatgacaa aaggtacacc acatattcaa ccaaatggag
      781 taaaaattgc taaaacagta ttaatgcctg gcgatccgct acgcgcaaaa tatattgctg
      841 ataatttttt agaaaatgtt gaacaattta acgatgtacg taacatgttt ggttacactg
      901 gtacatataa aggtaaagaa gtttctgtaa tgggttctgg tatgggtatt ccaagtattg
      961 gtatttactc atatgagtta tacaacttct ttgatgtaga tacaatcatt cgtatcggtt
     1021 cttgtggcgc attacaagaa aatgttaact tatacgatgt tattattgca caagctgcat
     1081 caactaattc aaattatgta gatcaataca atattccagg tcatttcgcg cctatcgctg
     1141 acttcgagtt agtaactaaa gctaaaaatg tcgctgacca aatcggtgct actacacacg
     1201 taggtaacgt attatcttct gatacatttt acaatgccga tccaacattc aatgatgctt
     1261 ggaaaaaaat gggtatttta ggtatcgaaa tggaatcagc tggtttatat ttaaatgcga
     1321 ttcatgctgg taaaaaagca cttggtattt tcacagtaag tgatcatatt ttacgtgacg
     1381 aagctactac acctgaagaa cgtcaaaatt catttacaca aatgatggaa atcgctttag
     1441 aaatcgcaga gtaacttatt taaattgact ttaattgctc tttaacaatg cgattaaact
     1501 caaaaagcca acacattctg ggcgtatccc catttatgtg ttggctttta tttatattat
     1561 tacttatctg tagattagct taagtaagat ttaaacatcc aattatgttt atctactgat
     1621 gtttgcatac ctataaacat atcttctgat acatcatcgc cagcattacc agcaatttcg
     1681 attgcgtttt ctaattgttt tgagatattt gtgaagtctt gtgataattc ttcaaccatt
     1741 tgttctgcag agtaaccttt cgcagcttct ttaacaattg attgctctaa gcattcagtt
     1801 aatgtaccta cagggtttcc tcctaccgct aaaattcttt cagctaattc gtctacatat
     1861 tggcttgctt cattatataa ttcttcaaat ttaacgtgta atgagaagaa gttaggtcct
     1921 ttcacatacc agtggaaatt atgtagcttt gtgtaagcta ctgtccagtt tgctacttgt
     1981 tgattcaatt cttttacaac atcttgttga ttactcatat ttaatacact ccttaaaatt
     2041 gtctacgtct tgcttgttta tattaattat tatattataa tgattctaat ctgtaaagtg
     2101 tttttactct cttttttata attattctaa tctaaatgta aggtgttccg ctttttaaat
     2161 gtctaaattt taattcatca tctagtatat ctattatcaa ttagcaagta tgttttatca
     2221 atcagtaagt tttactttcg cattatcatc taaaaagaac gatttttcat aatcatttgg
     2281 ttggtgccat gttgttttcc acattctatt tctattatta gcaaacattc gataaccgaa
     2341 attacggact ggtttaggta caagccataa acctacgccc aataatttag ttcgattagg
     2401 tagtgctgtg attagtttaa taatcgcctg cgattcaaaa tatatacgat cacctttctg
     2461 taaaatcaca ctatttttat ttgccgcttc tggatgttgt ttaaaaaatt gttgtccaac
     2521 ttcacctttt aacgtggcaa attgataatt tcttggtagg tcatgttgaa ttaaccaaat
     2581 gacatagtta taacaatata tacagtttcc atcataatat acgattggca taatttattc
     2641 gctcctttaa tagtacttat agtcattttt cctaatttaa aaacatttaa acaaggttgt
     2701 cacttgataa gtcctcgcga cacgaaattg ccataaaatt tatttttcag ttttatattt
     2761 attgtat
//
LOCUS       DAOBBH010000230         2765 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.230, whole genome shotgun sequence.
ACCESSION   DAOBBH010000230 DAOBBH010000000
VERSION     DAOBBH010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.230"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..2144)
                     /gene="fnbB"
                     /locus_tag="P6L01_002434"
     CDS             complement(<1..2144)
                     /gene="fnbB"
                     /locus_tag="P6L01_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbB"
                     /protein_id="HDP3031474.1"
                     /translation="MKSNLRYGIRKHKLGAASVFLGTMIVVGMGQEKEAAASEQNNTT
                     VEESGSSATESKASETQTTTNNVNTIDETQSYSATSTEQPSKSTQVTTEEAPTTVQAP
                     KVETEMKSQEDLPSEKVADKETTGTQVDIAQPSNVSEIKPRMKRSADVTAVSEKEVAE
                     EAKATGTDVTNKVEVTESSLEGHNKDSNIVNPHNAQRVTLKYKWKFGEGIKAGDYFDF
                     TLSDNVETHGISTLRKVPEIKSSTEDKVMANGQVINERTIRYTFTDYINNKKDLTAEL
                     NLNLFIDPTTVTKQGSQKVEVTLGQNKVSKEFDIKYLDGVKDRMGVTVNGRIDTLNKE
                     EGKFSHFAYVKPNNQSLTSVTVTGQVTSGYKQSANNPTVKVYKHIGSDELAESVYAKL
                     DDTSKFEDVTEKVNLSYTSNGGYTLNLGDLDNSKDYVIKYEGEYDQNAKDLNFRTHLS
                     GYHKYYPYYPYYPYYPVQLTWNNGVAFYSNNAKGDGKDKPNDPIIEKSEPIDLDIKSE
                     PPVEKHELTGTIEESNDSKPIDFEYHTAVEGAEGHAEGIIETEEDSIHVDFEESTHEN
                     SKHHADVVEYEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTVEDTKEYTTE
                     SNLIELVDELPEEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIDEIEENSHVD
                     IKSELGYEGGQNSGNQSFEEDTEEDKPKYEQGG"
BASE COUNT          750 a          515 c          386 g         1114 t
ORIGIN      
        1 ccaccttgtt catatttagg tttatcttct tctgtgtctt cctcgaatga ctgattaccg
       61 ctattttggc caccttcata acctaattca ctcttaatat caacgtggct attttcttcg
      121 atttcatcaa tcacgccata attaccgtga ccattttcag ttcctaaacc agaatgagaa
      181 atatgatgat tgttttcagt aatttcctcg attggccctt gtgcttgacc atgttcttca
      241 ggtaattcat ccactaattc aatcagatta ctttcagttg tatattcttt cgtatcttca
      301 actgttgtat ggtcgctcac tgcgccagtt acaatacctt ttgtagactc ttcgtcaaat
      361 tcaactaagt tagactcagt tgttacttgg ccaccacctg ggtttgtatc ctcttcatat
      421 tcaacaacat cagcgtgatg ttttgaattt tcatgtgtag attcttcaaa atccacatga
      481 atagaatctt cttcagtttc aataatacct tctgcatgac cttctgcacc ttcaacagct
      541 gtatgatatt caaaatcaat tggcttagaa tcgttacttt cttcgattgt accagtcaat
      601 tcatgcttct ccactggtgg ctctgattta atgtctaaat caattggttc actcttctca
      661 atgataggat catttggttt atctttacca tcgcctttag cattattaga gtaaaatgca
      721 acaccgttgt tccaagttaa ttgaactgga taatacgggt aataaggata gtatgggtag
      781 tatttatgat atcctgaaag atgtgttcgg aaatttagat ccttagcatt ttgatcatat
      841 tcaccttcat atttaattac atagtctttc gaattatcta aatcgccaag gttcaatgtg
      901 tacccaccat tacttgtgta agatagattt actttttcag tcacatcttc aaatttactg
      961 gtatcatcaa gctttgcata aacactttca gctaattcat ctgaaccaat gtgtttatat
     1021 actttgactg ttggattatt agcactttgt ttatatccag atgttacttg accagttact
     1081 gtgacagaag ttaacgactg gttgttaggc ttcacatatg caaaatggct aaatttaccc
     1141 tcttctttat tcaaagtatc aatacgacca ttaacagtaa cacccattct atctttaacg
     1201 ccgtctaaat atttgatatc aaattctttt gagactttat tttgacctag tgttacttca
     1261 actttttgac tcccttgctt tgtcactgtt gttgggtcaa tgaataggtt taagtttaat
     1321 tcagcagtta aatctttttt gttatttata taatcagtaa atgtatagcg aattgtacgt
     1381 tcatttataa cttgaccatt tgccataact ttatcttctg ttgaactttt tatctccgga
     1441 actttacgca gtgttgatat accatgtgtt tcaacattat cacttaatgt gaaatcaaaa
     1501 taatctcctg ccttaattcc ttctccaaat ttccatttgt attttaaagt tactctttga
     1561 gcattatgcg gattaacaat attcgaatct ttattatgtc cttctaaaga gctttcagta
     1621 acttccactt tatttgttac atctgtacct gtcgctttag cttcttccgc tacttctttc
     1681 tctgaaactg ctgtaacgtc agctgatctt ttcattcttg gtttaatttc tgagacgtta
     1741 cttggttgag ctatgtcaac ttgagttcct gtagtttcct tatcagcaac tttttctgat
     1801 ggtaaatctt cttgtgattt catttcggtt tctacttttg gtgcttgcac agttgttggt
     1861 gcttcttctg ttgttacttg agttgatttt gatggttgct cagttgatgt cgcgctgtat
     1921 gattgtgttt catctattgt attaacgtta tttgtagttg tttgtgtttc gcttgcttta
     1981 ctttcagtag ctgaactccc actttcctct actgtagtat tgttttgttc cgatgctgca
     2041 gcttcttttt cttgtcccat tccaacaacg atcattgttc ctaagaatac tgaggccgct
     2101 cccaatttgt gttttcttat gccgtatcta agattgcttt tcactataat attctccctt
     2161 aaatgcaaaa ttcatttatt tttaaaactc aataaatgca attctatatt gttcggtttt
     2221 taaaagcaat gaaaaaaagc gagttaataa aaagttaaga ttgttgttaa ctttatgtat
     2281 aatgagtttt ttattattta taactcacat atatattgca tgcaaaactc ttgaatccct
     2341 tgatataaca ggcttgtatt ttttatactt attttttaaa ttaaattcaa attcatctaa
     2401 tttaaagcaa tatactaaag catacataat aatcgcctgt acaatgcatc attaacaagt
     2461 cactgaaatg cctttcattg tattaataac gtcattataa ttttatatcg ttcggttttt
     2521 gttttatttt aatgattatt tatacagaaa cagccgtatt tcaagacgac attttaagtt
     2581 taactaaatt tgcatctagt taataattgc atttatcaaa tttgtcttat tgattcaatc
     2641 taatttgtac taacaaacta gttttaaatt ctaactttat ctctcatttt gtaatcaatc
     2701 atcagacata aagcgcttca gcaatcagaa aacactctaa ttttctatta gaaatttgat
     2761 ttaat
//
LOCUS       DAOBBH010000231         2736 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.231, whole genome shotgun sequence.
ACCESSION   DAOBBH010000231 DAOBBH010000000
VERSION     DAOBBH010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.231"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            64..835
                     /gene="seu"
                     /locus_tag="P6L01_002435"
                     /pseudo
     CDS             64..835
                     /gene="seu"
                     /locus_tag="P6L01_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031927818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type C1/U"
     gene            874..1629
                     /gene="sen"
                     /locus_tag="P6L01_002436"
     CDS             874..1629
                     /gene="sen"
                     /locus_tag="P6L01_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type N"
                     /protein_id="HDP3031475.1"
                     /translation="MRLFYIAAIIITLLCLINNNYVNAEVDKKDLKKKSDLDSSKLFN
                     LTSYYTDITWQLDESNKISTDQLLNNTIILKNIDISVLKTSSLKVEFNSSDLANQFKG
                     KNIDIYGLYFGNKCVGLTEEKTSCLYGGVTIHDGNQLDEEKVIGVNVFKDGVQQEGFV
                     IKTKKAKVTVQELDTKVRFKLENLYKIYNKDTGNIQKGCIFFHSHNHQDQSFYYDLYN
                     VKGSVGAEFFQFYSDNRTVSSSNYHIDVFLYKD"
     gene            1912..2688
                     /gene="seg"
                     /locus_tag="P6L01_002437"
     CDS             1912..2688
                     /gene="seg"
                     /locus_tag="P6L01_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031927817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type G"
                     /protein_id="HDP3031476.1"
                     /translation="MKKLSTVIIILILEIVFHNMNYVNAQPDPKLDELNKVSDYKNNK
                     GTMGNVMNLYTSPPVEGRGVINSRQFLSHDLIFPIEYKSYNEVKTELENTELANNYKD
                     KKVDIFGVPYFYTCIIPKSEPDINQNFGGCCMYGGLTFNSSENERDKLITVQVTIDNR
                     QSLGFTITTNKNMVTIQELDYKARHWLTKEKKLYEFDGSAFESGYIKFTEKNNTSFWF
                     DLFPKKELVPFVPYKFLNIYGDNKVVDSKSIKMEVFLNTH"
BASE COUNT         1102 a          290 c          416 g          928 t
ORIGIN      
        1 gtacgttaaa tattccctgt aatacatcaa atttataaaa aattaaaata tggagttgtt
       61 gtaatgaagt tatttgcctt tatcttcata tgtgttaagt cttgcagctt actatttatg
      121 ttaaatggca atcctaaacc agaacaattg aataaagcga gtgaattcac tggtctaatg
      181 gataatatga ggtatttgta tgatgataaa cacgtatcag aaacaaacat taaatcccaa
      241 gaaaagtttt tacaacatga tttattattt aaaataaatg gttctaaaat tttaaaaaca
      301 gaatttaata ataaaagcct ttcggataaa tataaaaata aaaacgtaga tttgtttggg
      361 acaaactatt ataatcaatg ctatttttca ttggataata tggaattaaa tgatggtaga
      421 ttaattgaaa aaaacgtgta tgtatggcgg tgtgaccgag catgatggaa atcaaataga
      481 taaaaataat tcaactgata actctcataa tatcttaatt aaagtttatg aaaacgagag
      541 aaattcatta tcttttgata tacctactaa taagaaaaac ataacagcac aagaaataga
      601 ttataaagtt agaaactatt tacttaagca taaaaattta tatgaattta acagttcgcc
      661 ttatgagact ggctatataa agtttatcga aggaagtggt cattcttttt ggtatgattt
      721 gatgcctgaa tctggtaaaa aattttatcc gactaaatat ttactaattt ataatgataa
      781 taaaacagtt gagagtaaat ctattaatgt agaagttcat ttaaccaaaa aataattgag
      841 ggagagaata ttataaaaaa tattaaaaag cttatgagat tgttctacat agctgcaatt
      901 ataataactt tattatgtct tattaataat aattatgtta atgctgaagt agacaaaaaa
      961 gatttaaaga aaaaatctga tctagatagt agtaagttat ttaatttaac aagctattat
     1021 actgatataa cgtggcaatt agacgagtca aataaaatta gtacagatca actactgaat
     1081 aatactataa tattaaaaaa tattgatata tccgtactta aaacttctag tttgaaagtt
     1141 gagtttaact catcagattt agcaaatcaa tttaaaggaa aaaatataga tatttatgga
     1201 ctgtattttg gaaataaatg tgtaggctta actgaagaaa aaacatcatg cttatacgga
     1261 ggagttacga tacatgatgg aaatcaatta gatgaagaga aagttatagg cgttaatgta
     1321 tttaaagatg gtgtccaaca agaaggtttt gttataaaaa ccaaaaaggc taaagtaaca
     1381 gtacaagaat tagacactaa agttcgattt aaattagaaa atttatataa aatatacaat
     1441 aaagataccg gtaacataca aaaaggatgc attttctttc attctcataa tcatcaagat
     1501 caatcatttt attatgattt atataacgta aaaggttcag tgggagcaga gttttttcaa
     1561 ttttatagtg ataatagaac agttagctca tctaattatc atattgatgt atttttatat
     1621 aaagattaag tgattgatga ttatcaactg aatcatgaaa ttaaaccatt ttatgagagg
     1681 aaaatgattt gaattgtagt aaaatttgaa atttatatat tatcttgaat acggattcaa
     1741 aattatttgt tgtaattagt aataatgata aatagaattt taatttattc tgctagtgaa
     1801 tttttagttt taaaacaatg ctatcgacac actacaacct gaactatcta taagcgtgaa
     1861 tttataataa ggttcattgt caaatagact gaataagtta gaggaggttt tatgaagaaa
     1921 ttatctactg taattattat tttgattcta gaaatagttt ttcataatat gaattatgtg
     1981 aatgctcaac ccgatcctaa attagacgaa ctaaataaag taagtgatta taaaaataat
     2041 aagggaacta tgggtaatgt aatgaatctt tatacgtctc cacctgttga aggaagagga
     2101 gttattaatt ctagacagtt tttatctcat gatttaattt ttccaattga gtataagagt
     2161 tataatgagg ttaaaactga attagaaaat acagaattag ctaacaatta taaagataaa
     2221 aaagtagaca tttttggcgt tccatatttt tatacatgta taatacctaa atctgaaccg
     2281 gatataaacc aaaattttgg aggttgttgt atgtatggtg gtcttacatt taatagttca
     2341 gaaaatgaaa gagataaatt aattactgta caggtaacaa tcgacaatag acaatcactt
     2401 ggatttacaa taactacaaa taagaatatg gttactattc aggaactaga ttacaaagca
     2461 agacactggc tcactaaaga aaaaaagcta tacgagtttg atggttctgc atttgaatct
     2521 ggatatataa aatttactga aaagaacaat acaagttttt ggtttgactt atttcctaaa
     2581 aaagaactag taccttttgt tccatataag tttttaaata tttacggaga taataaagtt
     2641 gttgattcta agagtattaa aatggaagta tttcttaata ctcactgata agtatatttg
     2701 atatcactat acaatcataa agtgcgtttt tcgaac
//
LOCUS       DAOBBH010000232         2699 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.232, whole genome shotgun sequence.
ACCESSION   DAOBBH010000232 DAOBBH010000000
VERSION     DAOBBH010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.232"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(141..1184)
                     /gene="recA"
                     /locus_tag="P6L01_002438"
     CDS             complement(141..1184)
                     /gene="recA"
                     /locus_tag="P6L01_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDP3031477.1"
                     /translation="MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNVGRRVSTTSTGSV
                     TLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEY
                     AQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGD
                     THVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYS
                     SVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDL
                     GVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQIKEEIDRKLREKLGISDGDVE
                     ETEDAPKSLFDEE"
     gene            complement(1349..2500)
                     /locus_tag="P6L01_002439"
     CDS             complement(1349..2500)
                     /locus_tag="P6L01_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HDP3031478.1"
                     /translation="MSIAIIAVGSELLLGQIANTNGQFLSKVFNEIGQNVLEHKVIGD
                     NKKRLESSVRHALEKYDTVILTGGLGPTKDDLTKHTVAQIVGKDLVIDEPSLKYIESY
                     FEEQGQEMTPNNKQQALVIEGSTVLANHHGMAPGMMVNFENKQIILLPGPPKEMQPMV
                     KNELLSHFINHNRIIHSELLRFAGIGESKVETILIDLIDKQTNPTIAPLAGSHEVYIR
                     LTANADSKEQAQSLIQPVKQEILDRIGEYYYGSDDTLIEQAVIKKIHEPFVIYDGITN
                     GALYHRLKEVDLNDVLKGMINHNENFVDINKPIEQQLKDAVQFVNKLFNVSSAIILLE
                     YDGVVHIGYDNNFEFKTEQFKMSKSRNLLKNRSQNYVLIRLLNWLRTTN"
BASE COUNT          843 a          516 c          354 g          986 t
ORIGIN      
        1 aaaataaaaa accttatagt ctcgatttgt agtgtatccc ataaagttag atattattct
       61 agctttatga gatgaatatc actttcaatc aatcctataa ggatatttgc taagtttaac
      121 tatagatata aatttgtgta ctattcttcg tcaaataatg actttggtgc atcttctgtt
      181 tcttcaacat caccatcaga tatacctaat ttttctctca atttacgatc aatttcttct
      241 ttaatttgtg gattttcttt caagtacatt ttaacatttt ccttaccttg acccattcgt
      301 tcgccattgt aagaatacca tgctcctgat ttatcaacga tgtcgttttc aacacctaaa
      361 tcaataagtt caccctcttt agaaatacct tgtccataca taatatcaac ttcagctact
      421 ctaaatggtg gtgccacttt atttttaacg actttaattt tagttctatt acctacaatt
      481 tcttgtcctt gtttaagctg ttctgcacga cgtacttcta gtcttactga actatagaat
      541 tttaatgcac gtccacctgg tgtagtctct ggattaccga acataacacc aactttttca
      601 cgaatttggt tgatgaaaat agcagttgta tttgatttag aaatagcacc tgaaagttta
      661 cgtaacgctt gtgacattaa acgagcttgt aaaccaacgt gagtgtctcc catttctcct
      721 tcaatttcag ctttaggtgt taaagcagca actgagtcta caactacaat atcaactgca
      781 ccacttctaa caaatgcttc ggcgatttca agaccttgtt caccatgatc cggttgcgat
      841 aaatataaat tatcgatatc tacgcctaat gcttgagcat attctggatc taaagcatgt
      901 tcagcatcga taaatgctgc caccccgcca ttactttgta cttcagcaat agcgtgaagc
      961 gctactgttg tcttaccaga actttcagga ccataaattt caataattcg tcctttagga
     1021 tagccaccta cacctagcgc attatctaat gtaactgaac cagttgatgt agttgaaact
     1081 cggcgaccta cattgtcacc caacttcatt acggcacctt taccgaaaga tttctccata
     1141 tttttaatta ctgtatctaa agctttttga cgatcgttat ccaaagcgag acctcctaat
     1201 tgaaattgct atcttttaat tatcttatca atactataca aggattcaaa ataaaataca
     1261 agtgttttgc gaatatttgt tcgtataaaa tcttgaaatc aaaacgcaaa aacgaacaaa
     1321 cgtgcttata tttttatcga taatacaatt aatttgttgt tctaagccaa tttaataatc
     1381 ttatgagcac ataattttga cttctgttct ttaataaatt tctagattta gacattttaa
     1441 attgctcagt tttaaattca aagttattat catagcctat atggactaca ccatcatact
     1501 ctaatagaat aattgctgat gacacattaa acaatttatt aacaaattgc actgcatctt
     1561 ttaattgctg ctcaataggt ttattaatat caacaaagtt ttcattgtga ttaatcatac
     1621 cctttagaac atcgtttaaa tccacttctt tcaatcgatg atataaagca ccattagtaa
     1681 taccatcata tattacaaaa ggttcatgaa ttttctttat tacagcttgc tcaattaatg
     1741 tgtcatctga accataataa tattctccaa tacgatcaag aatttcttgt ttaacaggtt
     1801 gaatcaatga ttgtgcttgt tcttttgagt cggcatttgc agtcaatcta atatatactt
     1861 catgacttcc cgccaaaggc gcaatcgtag gattagtctg tttatcgata agatctatta
     1921 atattgtttc tactttagat tcacctattc ccgcaaatct taatagttca gaatgtataa
     1981 ttcgattatg gtttataaaa tgtgacaaca attcattttt caccattggt tgcatttctt
     2041 tcggtggacc tggtaataaa ataatttgtt tgttttcaaa attcaccatc attcctggag
     2101 ccatgccatg atgatttgct aatacagttg aaccttcaat tactaaagcc tgttgtttat
     2161 tattaggtgt catttcttgt ccttgttcct caaaatagct ttcaatatat tttaaagaag
     2221 gctcatcaat aactaaatct ttaccaacaa tctgggccac tgtatgcttc gttaagtcat
     2281 ctttcgtagg acctaagcca cctgttaaaa taacagtatc atatttttct aacgcatgac
     2341 gtacacttga ttctaaacgt tttttattat ctccaataac tttatgttct aatacatttt
     2401 gtccaatttc attaaatact ttagatagaa attgtccgtt ggtattagcg atttgaccta
     2461 gcaatagttc tgagcctaca gcaataatgg caattgacat ttatatcccc cttgtatcgt
     2521 ttaattcttt tttcatgtat tccatttaaa aattcacatc aattttagat tcaatgtatc
     2581 agatgctaca acacatttgc ctaaaaaatg aacaaatatt gttttacaat atatcattat
     2641 aaacaattgc gtggctaatt tctaaaattg ggattaaaaa aactagattc catatgaaa
//
LOCUS       DAOBBH010000233         2693 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.233, whole genome shotgun sequence.
ACCESSION   DAOBBH010000233 DAOBBH010000000
VERSION     DAOBBH010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.233"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            12..719
                     /gene="splA"
                     /locus_tag="P6L01_002440"
     CDS             12..719
                     /gene="splA"
                     /locus_tag="P6L01_002440"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplA"
                     /protein_id="HDP3031479.1"
                     /translation="MNKNVMVKGLTALTILTSLGFAENISNQPHSIAKAEKNVKEITD
                     ATKAPYNSVVAFAGGTGVVVGKNTIVTNKHIAKSNDIFKNRVAAHYSSKGKGGGNYDV
                     KDIVEYPGKEDLAIVHVHETSTEGLNFNKNVSYTKFAEGAKAKDRISVIGYPKGAQTK
                     YKMFESTGTINHISGTFIEFDAYAQPGNSGSPVLNSKHELIGILYAGSGKDESEKNFG
                     VYFTPQLKEFIQNNIEK"
     gene            844..1566
                     /gene="splB"
                     /locus_tag="P6L01_002441"
     CDS             844..1566
                     /gene="splB"
                     /locus_tag="P6L01_002441"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplB"
                     /protein_id="HDP3031480.1"
                     /translation="MNKNVVIKSLATLTILTSVTGIGTTLVEEVQQTAKAENNVTKIQ
                     DTNIFPYTGVVAFKSATGFVVGKNTILTNKHVSKNYKVGDRITAHPNSDKGNGGIYSI
                     KKIINYPGKEDVSVIQVEERAIERGPKGFNFNDNVTPFKYAAGAKAGERIKVIGYPHP
                     YKNKYVLYESTGPVMSVEGSSIVYSAHTESGNSGSPVLNSNNELVGIHFASDVKNDDN
                     RNAYGVYFTPEIKKFIAENIDK"
     gene            1624..2343
                     /gene="splC"
                     /locus_tag="P6L01_002442"
     CDS             1624..2343
                     /gene="splC"
                     /locus_tag="P6L01_002442"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplC"
                     /protein_id="HDP3031481.1"
                     /translation="MNKNIVIKSMAALAILTSVTGINAAVVEETQQIANAEKNVTQVK
                     DTNNFPYNGVVSFKDATGFVIGKNTIITNKHVSKDYKVGDRITAHPNGDKGNGGIYKI
                     KSISDYPGDEDISVMNIEEQAVERGPKGFNFNENVQAFNFAKDAKVDDKIKVIGYPLP
                     AQNSFKQFESTGTIKRIKDNILNFDAYIEPGNSGSPVLNSNNEVIGVVYGGIGKIGSE
                     YNGAVYFTPQIKDFIQKHIEQ"
     gene            2464..>2693
                     /gene="splD"
                     /locus_tag="P6L01_002443"
     CDS             2464..>2693
                     /gene="splD"
                     /locus_tag="P6L01_002443"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplD"
                     /protein_id="HDP3031482.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTVVDGIQQTAKAENSVKLIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVT"
BASE COUNT         1056 a          379 c          498 g          760 t
ORIGIN      
        1 ggaggtaaaa tatgaataaa aatgtaatgg ttaaaggttt aactgcttta actattttaa
       61 catctcttgg tttcgctgaa aatatttcta atcagcctca ttcaattgcc aaagcagaaa
      121 agaatgtcaa agaaattacc gatgcaacta aggcaccata caattcagtg gtagcatttg
      181 cgggtggtac tggtgtagtt gttggtaaaa atacaatcgt aactaacaaa catatcgcta
      241 aaagtaatga tatttttaaa aatagagtag cagcacatta ttcgagtaaa ggtaaaggcg
      301 gaggaaacta cgacgttaaa gacattgtag aatatcctgg aaaagaagac cttgcgatag
      361 ttcatgttca tgaaacaagt acagaaggtt tgaattttaa taagaacgtt agttatacaa
      421 aatttgcaga aggagcaaaa gcgaaagata gaatttctgt tattggttat ccaaagggtg
      481 cacaaacaaa atataaaatg tttgaatcga caggaacgat taaccatatc agtggaacgt
      541 ttatagaatt tgatgcgtat gcacaaccag gtaattcagg atctcctgta ttgaattcta
      601 aacatgaact gattggtatt ttatatgcag gtagtggaaa agatgaatct gaaaagaatt
      661 tcggtgttta tttcacacca caattaaaag aatttattca aaataatatt gaaaaataag
      721 tatcatcaat tcattcgtga agttgatttt ttaaagagtg attaagaaaa cggttacata
      781 accaactaaa tatactgaat tatgtatcta gatatcaaaa taattaaaaa agaggaactt
      841 aaaatgaaca aaaacgtagt catcaagagt ttagcaactt taacaatttt aacatctgta
      901 acaggtattg gaacaacatt ggttgaggaa gttcaacaaa ctgccaaagc agaaaataat
      961 gtcacaaaaa ttcaagatac taatattttt ccatatactg gtgtagttgc ttttaaaagt
     1021 gcaactggat ttgtagttgg aaagaatact attttaacaa ataaacatgt gtcgaaaaat
     1081 tacaaagtgg gcgatcgtat tactgcacat ccaaatagtg ataaaggtaa tggtggtatt
     1141 tattcgatta aaaagattat taattatcca ggtaaagaag atgtatcagt cattcaagtt
     1201 gaagagcgtg caatagaacg tggaccaaaa ggctttaatt ttaatgataa tgtaacgcca
     1261 ttcaaatatg cggcaggggc taaagctggt gagcgaatta aagtgattgg ttatccacac
     1321 ccatacaaaa ataaatatgt tttatatgag tcaactggcc ctgtgatgtc agtagaaggt
     1381 agcagtattg tatattcagc gcatactgaa agcggaaact ctggatcacc tgtattaaac
     1441 agcaacaacg aattagttgg tattcatttt gcttctgatg taaaaaatga tgataacaga
     1501 aatgcatatg gcgtctactt tacaccagaa attaaaaaat tcattgcaga aaacatagat
     1561 aaataaacaa attgacttta aacgagcgtt gcaacatatc tcgaattgta aaggagcttg
     1621 aaaatgaata aaaatatagt cattaaaagc atggcagcat tagccattct aacctcagta
     1681 actggaataa atgctgcagt cgttgaagag acacaacaaa tagcaaatgc agagaagaat
     1741 gttacgcaag ttaaagatac aaataatttt ccatataatg gcgtcgtttc atttaaggat
     1801 gcgacaggtt ttgtaattgg aaaaaataca attatcacca ataaacatgt atcaaaagat
     1861 tataaagttg gcgatagaat tactgcccat ccaaacggtg acaaaggaaa tggtggtata
     1921 tataaaatta aaagcatttc tgattatccg ggtgatgaag acatctctgt catgaatatt
     1981 gaagaacaag cagttgaacg tggaccaaaa ggctttaatt ttaatgaaaa tgtccaagca
     2041 ttcaattttg cgaaagatgc taaagttgat gacaaaatta aagttattgg ttacccatta
     2101 cctgctcaaa atagttttaa acagtttgaa tctacaggaa ctataaaaag aatcaaagac
     2161 aatattttaa attttgatgc atacattgaa cccgggaatt caggatcacc agttctaaat
     2221 tctaacaatg aggtcatagg tgtggtgtat ggcggtattg gaaaaattgg ttctgaatat
     2281 aatggtgccg tatactttac gcctcaaatc aaagatttta ttcaaaagca cattgaacaa
     2341 taaacaaatt taaatataca ccatgagcat gtgttcaata attttaatga aaaacatcgg
     2401 tcgaatataa cataaaaaaa cgtctatatc aaaagcatca tgaataaaca gaggagcaca
     2461 aaaatgaata aaaatataat catcaaaagt attgcggcat tgacgatttt aacatcaata
     2521 actggtgtcg gcacaacagt ggttgatggt attcaacaaa cagccaaagc agaaaatagt
     2581 gtgaaattaa ttaccaacac gaatgttgca ccatacagtg gtgttacatg gatgggcgct
     2641 ggaacaggat ttgtagttgg gaatcataca atcattacca ataaacatgt tac
//
LOCUS       DAOBBH010000234         2686 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.234, whole genome shotgun sequence.
ACCESSION   DAOBBH010000234 DAOBBH010000000
VERSION     DAOBBH010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.234"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            144..1289
                     /locus_tag="P6L01_002444"
     CDS             144..1289
                     /locus_tag="P6L01_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDP3031483.1"
                     /translation="MIRIQDNTIRDGMQQSNVRKSLIIKKEVLKQINKLNINSVEVGM
                     CTTIEDEFNIHQFRDILSPEKELVVLTRLNEKEIKKIVKLKIHNLVVKILLPISDLHI
                     KEKLNFSNKYYIQKIKDCLDILKKDKKGVDICFEDATRTSREKLKEYMEIISKYQVRT
                     VTFADTVGCSTPLEYGDIFNYFVKKYSNIIFSAHCHNDLGLATANTLTAILNGAKQIE
                     TTFLGIGERAGNAPIEEIITILTKKQIESTEFTLPDVYKTSINISKILDFQISENKPI
                     IGENIFKHESGIHQDGTKKNINMYQYLVPSDLGLKNSQVVQFPISNISSKKILHNKFK
                     SIVNTEEIDENISFYKLVNKFSPEVAPEDTVDLLQIIKRRSKDGNFK"
     gene            1273..1914
                     /locus_tag="P6L01_002445"
     CDS             1273..1914
                     /locus_tag="P6L01_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HDP3031484.1"
                     /translation="MEILNDSVKSFEKLYLQLQDGFPVIVPTDTNYNLCSLPNNDLCI
                     DKIFEYKKRSKDKPLSLFIDKPEDWKLYGDNQNTEIVDKLVEIFWPGPLNIILKNKTS
                     YNYMLNNSDSIAIGCVQNKTMRRFISYINSPIAITSANISGTADDILITENEAIKHMG
                     ENVRYMLRSQNKTNYKTSSTIIKVTDNKIELLREGDIKFEEIKERLGTGIIYE"
     gene            1907..>2686
                     /locus_tag="P6L01_002446"
     CDS             1907..>2686
                     /locus_tag="P6L01_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031485.1"
                     /translation="MNKLILGIYLYRIFSRAYFYLPFLLIYFLIQGYSIIQLEILMAS
                     YGIAAFLFSLYKEKCFKICNLKDSNKLVVSEIFKIIGLLLLLYQNQYLILVVAQILLG
                     LSYSMMAGVDTAIIKRNITNEKYVQNKSNSYMFLSLLISGIIGSYLYGINIKWPIIMT
                     GIFSILTIIIIRCTLVENRELNLIGETKGKIKKFLPEEKFWILHYSFLRALILGFFIG
                     FIPINIYNDLKLNNLQFISVLTCYTVMGYLSSRYLTKYLNYK"
BASE COUNT         1091 a          285 c          407 g          903 t
ORIGIN      
        1 aaatgacaaa tgtatagtac caatccaaat tcatgaactt acctctttca aaaaagttaa
       61 taattcatta gataatattt aatttttatt gcataaaaat aatattaata ttatatcaga
      121 ggttatcgag ggggtgaagt aatatgattc gaattcaaga taatacaata agggatggta
      181 tgcaacaaag taatgttcga aaaagtctaa ttataaaaaa agaagtattg aaacaaatta
      241 acaagttaaa tataaattct gttgaagtag gcatgtgtac aactatcgag gatgaattta
      301 atattcatca attcagagac attttaagtc ctgaaaaaga attagtagta ttgactaggc
      361 ttaatgaaaa agaaataaaa aaaatagtca aattgaaaat tcataattta gtggtaaaaa
      421 tactattgcc aatatctgac ttgcatataa aagaaaagct taatttttca aataaatatt
      481 atattcagaa aatcaaagac tgcttggata tattaaagaa agataaaaaa ggagtagata
      541 tttgttttga agatgcaaca aggacttcta gagaaaaatt gaaagaatac atggaaatta
      601 tttcaaaata tcaagttaga acagttacat ttgcggacac tgtaggatgt tcgacaccat
      661 tagaatacgg agatattttt aattactttg taaaaaaata ttctaacata attttttctg
      721 ctcattgtca taacgatcta gggttggcta ctgcaaatac attaactgca attttaaatg
      781 gtgcaaagca aatagaaact acatttttgg gaattggtga gagagcgggt aatgctccta
      841 ttgaggaaat aattactatt ttgacaaaaa aacaaataga aagtacggaa ttcactttac
      901 ccgacgtata taaaactagt attaatattt ctaaaattct cgattttcaa atatcagaaa
      961 acaaacctat aattggtgaa aatatattta aacatgaatc aggaattcat caagatggta
     1021 ctaaaaaaaa tataaatatg tatcaatatt tagttcctag tgatttagga ttgaaaaatt
     1081 cacaagttgt tcagtttcca ataagtaata tttctagtaa gaaaatcttg cacaataaat
     1141 ttaaatcaat agttaacact gaagaaattg atgaaaatat ttctttctat aaacttgtga
     1201 acaagttttc acctgaagta gcacctgaag atacagtgga tttacttcag ataataaaaa
     1261 ggaggagtaa agatggaaat tttaaatgat tctgtaaaaa gttttgaaaa actttattta
     1321 caattacaag atggttttcc agtgattgtt cctactgata ctaattataa tttatgtagt
     1381 ttaccaaaca atgatctttg tatcgataaa atatttgaat ataaaaagag atcgaaagat
     1441 aagccattat cattatttat tgataagcca gaggattgga aattgtatgg agataatcaa
     1501 aatacggaaa tagttgataa actagttgaa atattttggc ctggaccatt aaatattatt
     1561 ttaaaaaata aaacaagcta taattatatg ctcaataatt cagatagtat agctatagga
     1621 tgtgtgcaaa ataaaacgat gagaaggttt atttcatata ttaattcacc tatagcaatt
     1681 acttcagcaa atatctctgg aactgccgat gatattttaa taactgaaaa tgaagcaatt
     1741 aagcacatgg gcgaaaatgt aagatatatg ctaagaagtc aaaataaaac taactataaa
     1801 acatctagta cgattattaa agtgacagat aataaaattg aattattaag agaaggagat
     1861 ataaagtttg aagaaataaa agaaagacta ggtacaggta ttatttatga ataagttaat
     1921 acttgggatt tatttgtacc gaattttttc acgagcatac ttttatttac cgtttttatt
     1981 aatttacttt ttgattcaag gttattccat aatacaatta gaaatattaa tggcgtctta
     2041 tggcattgca gcatttttat tctctctata caaagagaag tgttttaaaa tttgtaactt
     2101 aaaagattct aataaattag ttgttagtga aatattcaaa atcatcggtt tattgttgtt
     2161 attatatcaa aatcaatatt taattttagt agtggcacaa atattattag ggttaagtta
     2221 ctcaatgatg gcgggtgttg ataccgcaat aattaaaaga aatataacaa atgagaaata
     2281 cgtacaaaat aagtcaaata gctatatgtt cctatcatta ttaatttcag ggattatagg
     2341 tagttatctt tatggaataa atattaaatg gcctataata atgactggta tattttcaat
     2401 tctaacaatt ataattattc gatgcacatt agttgaaaat agggaattaa atttaatagg
     2461 agaaacaaag ggaaagataa agaaatttct accggaagag aagttttgga tattgcatta
     2521 ttctttttta agagcgttaa tattaggatt ttttatagga tttattccaa ttaatatata
     2581 taatgattta aaactgaata atttacaatt tatttcagta ttaacttgtt acacagttat
     2641 gggttattta tcttcacgtt atttaactaa atacttgaac tataag
//
LOCUS       DAOBBH010000235         2684 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.235, whole genome shotgun sequence.
ACCESSION   DAOBBH010000235 DAOBBH010000000
VERSION     DAOBBH010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.235"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..1001
                     /gene="sspA"
                     /locus_tag="P6L01_002447"
     CDS             <1..1001
                     /gene="sspA"
                     /locus_tag="P6L01_002447"
                     /EC_number="3.4.21.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Glu-specific serine endopeptidase SspA"
                     /protein_id="HDP3031486.1"
                     /translation="FLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQSSKQQ
                     TPKIKKGGNLKPLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTFIASG
                     VVVGKDTLLTNKHVVYATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEGDLAI
                     VKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKITYLK
                     GEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQNIED
                     IHFANDDQPNNPDNPDNPNNPDNPNNPDEPNNPDNPNNPDNPDNGDNNNSDNPDAA"
     gene            1083..2264
                     /gene="sspB"
                     /locus_tag="P6L01_002448"
     CDS             1083..2264
                     /gene="sspB"
                     /locus_tag="P6L01_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain B"
                     /protein_id="HDP3031487.1"
                     /translation="MNSSYKSRVFNIISIIMVSMLILSLGAFANNNKAKADSHSKQLE
                     INVKSDKVPQKVKDLAQQQFAGYAKALDKQSNAKTGKYELGEAFKIYKFNGEEDNSYY
                     YPVIKDGKIVYTLTLSPKNKDDLNKSKEDMNYSVKISNFIAKDLDQIKDKNSNITVLT
                     DEKGFYFEEDGKVRLVKATPLPGNVKEKESAKTVSAKLKQELKNTVTPTKVEENEAIQ
                     EDQVQYENTLKNFKIREQQFDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSE
                     QDLPNCSTFPNQMIEYGKSQGRDIHYQEGVPSYEQVDQLTKDNVGIMILAQSVSQNPN
                     DPHLGHALAVVGNAKINDQEKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIG
                     Y"
     gene            2302..2631
                     /gene="sspC"
                     /locus_tag="P6L01_002449"
     CDS             2302..2631
                     /gene="sspC"
                     /locus_tag="P6L01_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin B"
                     /protein_id="HDP3031488.1"
                     /translation="MYQLQFINLVYDTTKLTHLEQTNINLFIGNWSNHQLQKSICIRH
                     GDDTSHNQYHILFIDTAHQRIKFSSIDNEEITYILDYDDTQHILMQTSSKQGIGTSRP
                     IVYERLV"
BASE COUNT         1056 a          468 c          414 g          746 t
ORIGIN      
        1 aatttttaaa agttagttct ttattcgttg caactttgac aacagcgaca cttgtgagtt
       61 ctccagcagc aaacgcgtta tcatcaaaag ctatggacaa tcatccacaa caaacgcagt
      121 caagcaaaca gcaaacacct aagattaaaa aaggcggtaa ccttaaacca ttagaacaac
      181 gtgaacacgc aaatgttata ttaccgaata acgatcgtca ccaaatcaca gatacaacga
      241 atggtcatta tgcacccgta acttatattc aagttgaagc acctactggt acatttattg
      301 cttccggtgt agttgtaggt aaagatactc ttttaacaaa taaacacgtc gtatatgcta
      361 cgcacggtga tcctcatgct ttaaaagcat tcccttctgc aattaaccaa gacaattatc
      421 caaatggtgg tttcactgct gaacaaatca ctaaatattc aggcgaaggt gatttagcaa
      481 tagttaaatt ctcccctaat gagcaaaaca aacatattgg tgaagtagtt aaaccagcaa
      541 caatgagtaa taatgctgaa acacaagtta accaaaatat tactgtaaca ggatatcctg
      601 gtgataaacc tgtagcaaca atgtgggaaa gtaaaggaaa aatcacttac ctcaaaggcg
      661 aagctatgca atatgattta agtacaactg gtggtaactc aggttcacct gtatttaatg
      721 aaaaaaatga agtgatcgga attcattggg gcggtgtacc aaatgaattt aatggtgcgg
      781 tatttattaa tgaaaatgta cgcaacttct taaaacaaaa tattgaagat atccattttg
      841 ccaacgatga ccaacctaat aacccagata atcctgataa ccctaacaat cctgataacc
      901 ctaacaaccc agatgaacca aataaccctg acaaccctaa caaccctgat aatccagaca
      961 atggcgataa caataattca gacaaccctg acgctgcata aacttcatct taatttagat
     1021 ttagtaaata gaagcaaagt cttagcaacg caaaatattt tgatttaaat ggagggtatt
     1081 atatgaatag ttcatataaa tctagagtat tcaatattat aagcatcata atggtttcaa
     1141 tgcttatttt atcactaggc gcatttgcta ataacaataa agccaaagcc gattcacact
     1201 ctaaacagct agaaattaat gttaagagtg acaaagtacc tcaaaaagta aaagatctag
     1261 cacaacaaca atttgctggt tatgctaaag cattagataa acaaagtaat gcaaaaactg
     1321 gtaaatatga acttggcgaa gcttttaaaa tttataaatt taatggtgaa gaagataata
     1381 gttactatta tccagttata aaagacggta aaattgttta tactttaaca cttagcccta
     1441 aaaataaaga tgatttaaac aaatcaaaag aagacatgaa ttacagtgtt aaaatttcaa
     1501 acttcatcgc taaagattta gaccaaatta aagataaaaa ttcaaatatc actgttctta
     1561 ctgatgaaaa agggttttat tttgaagaag atggcaaagt tagattagta aaagctacgc
     1621 cactacctgg taatgtaaaa gaaaaagaaa gtgctaaaac agtttcagca aaattgaaac
     1681 aagagttaaa aaatacagta acacctacta aagttgaaga aaacgaagcg atacaagaag
     1741 atcaagttca atatgaaaat acattaaaaa acttcaaaat tagagaacaa caattcgata
     1801 actcatggtg tgcaggattt agtatggcag cattattaaa tgcaactaaa aatacagaca
     1861 cttataatgc acatgatatt atgcgtacat tataccctga agtaagtgag caagaccttc
     1921 ctaattgctc aacattccct aatcaaatga ttgaatacgg taaatcacaa ggcagagata
     1981 ttcattatca agaaggcgta ccatcatatg aacaagttga tcaacttaca aaagataatg
     2041 taggaattat gattcttgca caaagtgtat ctcaaaaccc taatgatcca catttaggac
     2101 atgccctagc agttgttggt aatgctaaaa ttaatgacca agaaaaactt atttactgga
     2161 atccttggga tacagaatta tcaatccaag atgcagattc aagcctatta catttatcat
     2221 tcaatcgtga ttataactgg tatggttcaa tgataggtta ctaaaaagta atatagatat
     2281 tgattaaagg caggtaaaac tatgtatcaa ctacaattta taaatttagt ttacgacaca
     2341 accaaactca cacatctaga acaaaccaat atcaatttat tcattggtaa ttggagtaat
     2401 catcaattac aaaaatcaat ttgtatacgt catggcgatg atacaagtca caatcaatat
     2461 catattcttt ttatagatac ggcacatcaa cgcattaaat tttcatctat tgataatgaa
     2521 gaaatcactt atattcttga ttatgatgat acacagcata tcctcatgca aacgtcatcc
     2581 aaacaaggta ttggcacttc gcgcccaatc gtttatgagc gcttagtata actaatttaa
     2641 atgatttcac ttcataaagc gggttggcga gaattcaatt tctc
//
LOCUS       DAOBBH010000236         2634 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.236, whole genome shotgun sequence.
ACCESSION   DAOBBH010000236 DAOBBH010000000
VERSION     DAOBBH010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.236"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..423)
                     /locus_tag="P6L01_002450"
     CDS             complement(<1..423)
                     /locus_tag="P6L01_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDP3031489.1"
                     /translation="MLKKAKLILIAALVLSGCSTIENESKKDRNTETNTETKSVPEEM
                     EASKYVGQGFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGDGVE
                     VYVHCDDHDIVFNASIPFDKSIIDSDSSLRSEDKGDDMS"
     gene            complement(477..882)
                     /locus_tag="P6L01_002451"
                     /pseudo
     CDS             complement(477..882)
                     /locus_tag="P6L01_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829176.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(894..1790)
                     /locus_tag="P6L01_002452"
     CDS             complement(894..1790)
                     /locus_tag="P6L01_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HDP3031490.1"
                     /translation="MFKRTKLILIATILLSGCSTTNNESNNETKSVPEEMEASKYVGQ
                     GFQPPAEKDAIEFSKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGDGVEVFVHCDDH
                     DIVFNASIPFDKSIIESDSSLRSEDKGDDMSTLVGTVLSGFEYRAQKEKYDNLYKFLK
                     ENEKKYQYTGFTKEAINKTQNVGYQNEYFYITYLSRNLKEYRKYYEPLIHKKDKEFKE
                     GMQRARKELNYTANTDVVSTLFSTKKNFTKDNTVDDVIELSNKLKDKPNMPQKSQVTI
                     QLGKPSINTKKPFYDDINPIEG"
     gene            complement(1880..>2634)
                     /locus_tag="P6L01_002453"
     CDS             complement(1880..>2634)
                     /locus_tag="P6L01_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031491.1"
                     /translation="KQNIERIKNQNDIYGRQIAEIRSIMKQQDATILDGNFQINCSGE
                     NMVQGLVIPSNYLGRKMKILKDHIDDGIKKIADYVQSIYDEYASKIVDVIKYLINTIP
                     KIRKNLRHAIEMLNVKKKEFLSLIPNVTCNYIKTKLEELDNTLGKWEPFLNDLKAVSP
                     ILDNHLDDIVKNMKPLIVQMLFEPSHYDDMFILNTQAHARLDQMTQQFEVVCNGLNEN
                     EGQAIQTMDQSASLIRSNLIQVKEQLEKLAVY"
BASE COUNT          749 a          466 c          321 g         1098 t
ORIGIN      
        1 actcatatca tcgcctttat cctcacttct taatgagcta tcactgtcaa taattgattt
       61 gtcaaatgga atactcgcat taaatacgat atcgtggtca tcacaatgca cgtatacttc
      121 tacgccgtcg ccactaccta caacattcgt agctttaact tttagaccga agttatccat
      181 aaaaaattgt tcgccacgtt tagcaatttt atctttatgc ttcttcgcaa attcaatcgc
      241 atctttttct gcaggcggtt ggaatccttg gcctacatat tttgaagctt ccatttcttc
      301 tggtacagat ttcgtttctg tatttgtttc tgtatttctg tctttttttg attcattttc
      361 aatcgtggag caccctgaca gtactagtgc tgctattaag attaattttg cttttttaag
      421 catatctcat cacccattta tgcgctttta taagtttcat tttgcatata gttatactca
      481 cttaatttat ttgcatcttt tagttcacct atatataaat cttcaagtac atatacttgc
      541 gttaaaaaat ctattatttt aatttaatta ttcattaatt tagcattttg aagccaatca
      601 tctccaaaac ccgatgattt taatggatta tttggattaa ttgcctcttt agatgttcct
      661 tgatatatta ttgtcttttg accagttgga tctccctgat cattaagtag ctcgtaggtt
      721 tttatatcaa ctgcatcttt catattatcg ttatttttat cattaaaata atctatttgt
      781 ttgaaccgtt ttccatttac tttaaaatct tttttcattt atatcttgat aaactcaata
      841 tctgctcaat tctgctatgt ccctatcatt tattttaatc actttatatt cactcatcct
      901 tcaattggat ttatatcgtc ataaaacggc tttttagtat ttatacttgg ttttcctaat
      961 tgaatagtaa cttgagattt ttgaggcata ttgggtttat cttttaattt attactcaat
     1021 tcaatcacat catctactgt attgtcttta gtaaagtttt tcttagtaga aaaaagtgta
     1081 gaaacaacat cagtattagc agtatagttt agctcttttc tagctcgttg cataccttct
     1141 ttaaattctt tatccttttt atgaatcaat ggttcgtaat atttacgata ttcttttaaa
     1201 tttcttgata aatatgtaat ataaaaatat tcattttgat acccgacgtt ttgcgtcttg
     1261 ttaattgctt ctttagtaaa acctgtatat tgatatttct tttcattttc ttttaaaaat
     1321 ttatataaat tatcatactt ttctttttgc gctcgatatt caaagccact caacactgta
     1381 ccaactaaag tactcatatc atcgccttta tcctcacttc ttaatgagct atcactctca
     1441 attattgatt tgtcaaatgg aatacttgca ttaaatacga tgtcgtggtc atcacaatgc
     1501 acgaatactt ctacaccgtc gccactacct acaacatttg tagctttaac ttttagtcca
     1561 aagttatcca taaaaaattg ttcgccacgt ttagcaattt tatctttatg cttcttcgaa
     1621 aattcaatcg catctttttc tgcaggtggt tggaagcctt gtcctacata ttttgaagct
     1681 tccatttctt ctggcactga ttttgtttca ttgttggatt cgttattggt agttgaacat
     1741 cctgatagta gtatcgttgc tattaagatt agttttgttc ttttaaacat agctcatcac
     1801 ccatttatgt gtttgtaaaa cttttatgtt aaaaaaacta cttattctca aagaaaacaa
     1861 gtagcattta ataaattact tagtatacag ctagtttttc taattgttct ttaacttgaa
     1921 ttaagtttga ccgtattaga gaggcagatt gatccatcgt ttgaattgct tgtccttcat
     1981 tttcgttcaa gccattacaa acaacttcaa actgttgtgt catttgatca agacgtgcat
     2041 gagcttgtgt gtttaaaata aacatatcat cataatgtga tggttcaaat aacatctgta
     2101 caatcaaagg cttcatgttc ttaacaatat catctaaatg gttatctaaa attggtgaca
     2161 ctgcttttaa atcattaaga aatggctccc atttgcctaa agtattatct aattcttcta
     2221 atttagtttt aatataatta caagttacat taggaatcag ggacaaaaat tctttctttt
     2281 ttacatttaa catttcaatt gcatgtctta aattcttacg tattttggga attgtattaa
     2341 tcaaatattt tattacatca acaattttcg atgcatattc atcatatata ctttgaacat
     2401 agtctgctat ttttttaata ccatcatcga tatggtcttt taatattttc atttttcttc
     2461 ctaaataatt agaaggtata actagaccct gtaccatatt ttcgccgcta caattaattt
     2521 ggaaattccc atctaaaatt gttgcatctt gttgtttcat aatacttcta atttctgcaa
     2581 tttgcctacc ataaatatca ttttgatttt ttattcgctc tatattctgt ttca
//
LOCUS       DAOBBH010000237         2606 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.237, whole genome shotgun sequence.
ACCESSION   DAOBBH010000237 DAOBBH010000000
VERSION     DAOBBH010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.237"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(209..907)
                     /locus_tag="P6L01_002454"
     CDS             complement(209..907)
                     /locus_tag="P6L01_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HDP3031492.1"
                     /translation="MSVGILIFVISVIISIITTMRENSHKDRQNQKPPQKTSTDNEPK
                     KGGFFEEIERTFKEISEELNEEEKKSSKRKYDDTLPPLFDELPKEEPKSKPVVEPMAP
                     KKQPETKPMTEKPITVPKAEPVEQKHRPSRQDNSDEIRRQLEKSLRDDIKTIRTDIDR
                     EKEKQIAKMEKRARDIIEDKYLSERTKRLKLKQLLNSQNVEKDLTKSAFQFDKDEVIN
                     GMIWSEILAKPKQL"
     gene            complement(924..1913)
                     /gene="floA"
                     /locus_tag="P6L01_002455"
     CDS             complement(924..1913)
                     /gene="floA"
                     /locus_tag="P6L01_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500186.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HDP3031493.1"
                     /translation="MFSLSFIVIAVIIIVALLILFSFVPIGLWISALAAGVHVGIGTL
                     VGMRLRRVSPRKVIAPLIKAHKAGLALTTNQLESHYLAGGNVDRVVDANIAAQRADID
                     LPFERAAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMNGIEVKAKARITVRANIAR
                     LVGGAGEETIIARVGEGIVSTIGSSKHHTEVLENPDNISKTVLSKGLDSGTAFEILSI
                     DIADVDISKNIGADLQTEQALADKNIAQAKAEERRAMAVATEQEMKARVQEMHAKVVE
                     AESEVPLAMAEALRSGNISVKDYYNLKNIEADTGMRNAINKRTDQSDDESPEH"
     gene            complement(1931..2602)
                     /locus_tag="P6L01_002456"
     CDS             complement(1931..2602)
                     /locus_tag="P6L01_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDP3031494.1"
                     /translation="MESTAGDTWVEQVSNIIVQPIFTLILTCLTFLGFVYQLYSKKIN
                     AAGIIATLSLLILFLGFLIQGNVNMHSILIFSIGVILVVIELFVVGAVIGIIGMILIT
                     ISITTLGDNLLFMLANVIVALILTIVEWVILVKIFNRKIPFLDKVILKDSTNSESGYN
                     SHDNRSHLVGKTAQTVTDLRPAGIIFCENERIDAVSDGNFILRNKTVKILEVEGTRVV
                     VREVD"
BASE COUNT          787 a          503 c          374 g          942 t
ORIGIN      
        1 gcattgactt actcattttt ctttaattta aaataatcat ttatactttt caccttgtca
       61 taaaagcagt ttcaatttta gactaatcaa ttttttgctg taatttaaat aactaatatt
      121 tctcgaaaga ttctctttgt tttttaattt ttcttaacgt aaaaaaacaa gcaactacca
      181 ttacgatagt gcttgttttc aaaaaatttt ataattgttt tggtttagct aaaatttctg
      241 accatatcat accattgatt acttcatctt tatcaaattg gaacgctgat ttagtcaaat
      301 ctttttcgac attttgagaa ttaagcaact gctttaattt caaacgtttt gtacgttcag
      361 ataagtattt atcctcaata atatctctag cacgtttttc cattttagca atttgctttt
      421 ctttttctct atcaatgtca gtacgaatcg ttttaatatc atctctaagt gatttttcta
      481 attgacgtct aatttcgtca gaattatctt gtctagaagg tctatgtttc tgctccaccg
      541 gttctgcttt aggcactgta attggtttct ctgtcatcgg ttttgtttct ggttgttttt
      601 taggtgccat aggttctaca acaggtttcg atttaggctc ttcctttgga agttcatcga
      661 ataaaggtgg taacgtatca tcatattttc gtttcgatga tttcttttct tcttcattta
      721 attcttcact tatttcttta aacgttcgct caatttcttc aaaaaagcca cctttttttg
      781 gttcattatc ggtagatgtt ttttgaggtg gcttttgatt ttgtctatct ttatgactat
      841 tttcgcgcat agtagtaatg atagaaatga tcactgatat gacaaaaatt agaataccaa
      901 cactcattta atcacctctc gacttaatgt tcaggtgact catcatcact ttgatcagtt
      961 cgtttattaa ttgcatttct catgcctgta tcagcttcga tatttttcaa attataataa
     1021 tctttaacac tgatattacc tgaacgtaat gcttcagcca tagctaatgg tacttcagat
     1081 tcggcttcaa ctactttagc atgcatttct tgtacacgcg ctttcatttc ttgctcagtt
     1141 gctacagcca tagctctacg ttcttcagct tttgcttgtg caatattttt gtctgctaat
     1201 gcttgttcag tttgtaagtc tgcaccaata tttttactaa tatcaacgtc agcaatatca
     1261 attgataaaa tttcaaatgc agtacctgaa tctaaacctt tgcttaaaac tgttttagaa
     1321 atattatctg ggttttcaag tacttctgta tgatgcttac tagaaccaat tgttgaaacg
     1381 ataccttcac caacacgtgc gatgattgtt tcttcacctg caccaccaac aagtcgagca
     1441 atattagctc taactgtgat acgagctttg gctttcactt caataccgtt cattgctaca
     1501 cctgcgataa atggtgtttc aatgacttta ggattaacag acatttgaac cgcttctaat
     1561 acgtcacgtc ctgcaaggtc aattgcagca gcacgttcga aaggaagatc aatgtcagca
     1621 cgttgtgcag caatattagc atcaacaact ctgtcaacat ttcctcctgc tagataatgc
     1681 gattctaatt ggtttgttgt taatgctagt cctgctttgt gcgctttaat taatggcgct
     1741 ataacttttc ttggagatac acgacgtaaa cgcataccaa ccaatgtacc tataccaaca
     1801 tgaacgccag ctgctaacgc tgaaatccac aaaccaatcg gtacaaatga gaataaaata
     1861 agtaatgcaa ctattataat aactgctatt acgataaaac ttaaactaaa catggtatcg
     1921 ctccttttaa ttaatctact tccctcacaa ctactcttgt tccttcaact tcaaggattt
     1981 tcaccgtttt attgcgtaaa ataaagttgc catctgaaac agcatcaata cgttcatttt
     2041 cacaaaaaat aatccctgca ggtcgaagat ctgtaactgt ttgagcagtc tttcctacga
     2101 ggtgcgagcg gttatcatga gaattgtaac ctgactcaga attagttgaa tcttttaaga
     2161 taaccttatc caaaaacgga atctttctgt tgaaaatctt cactaatatc acccattcta
     2221 caatcgttaa aatcaaggca acgataacat tcgcaagcat aaatagcaaa ttatcaccga
     2281 gcgttgttat gcttatagtt atcagtatca tgccaataat accaattact gcaccaacta
     2341 caaataattc aattacaact aatataacgc caattgagaa tattaagata gaatgcatat
     2401 tgacatttcc ttggattaga aatcccaaaa ataaaataag taatgataat gtggcgataa
     2461 taccagctgc attgattttt ttagagtaaa gttgatatac aaatcctaag aatgtcaaac
     2521 aggttaatat taacgtaaaa ataggttgaa caattatatt actaacttgt tcaacccaag
     2581 tatctccagc cgttgattcc aagata
//
LOCUS       DAOBBH010000238         2563 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.238, whole genome shotgun sequence.
ACCESSION   DAOBBH010000238 DAOBBH010000000
VERSION     DAOBBH010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.238"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..954
                     /locus_tag="P6L01_002457"
     CDS             <1..954
                     /locus_tag="P6L01_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HDP3031495.1"
                     /translation="MPLLMDFIQQHSIDPSVIFSKFSNYYEFLLRYKKIDALLTENES
                     KNLVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKMLNEVKDITEDDIDTSLRIL
                     DFSFYNAGIEKIYGSPIIECNERMIRLSDAFTNALSNQTFKIFLEDLIELSKYNNEKY
                     QKGKNGLILYNKYSREDFSKIFNWNKNGSSVIMGYMIRSQEMPIFITYDKHEDISDST
                     KYEDEFLSQDELKWFTKSNRTLKSKEVQKILSHRAKGIKMYIFVQKKDDDGIYFYYLG
                     TAGYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYITN"
     gene            1261..2034
                     /locus_tag="P6L01_002458"
     CDS             1261..2034
                     /locus_tag="P6L01_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDP3031496.1"
                     /translation="MIHSRKLRLWLYLVLLAVFIGACGMKKEESSKDKQIKENFNKTL
                     SLYPTKNLEDFYDKEGFRDEEFEKGDKGTWIIHSKMTIETNGKNMESRGLVLYVDRNT
                     RTTKGEFIVRELWEDKKGYSRSKEKEYPVKMEHNKIIPTKPIADDKLRKEIEDFKFFV
                     QYGDFKDINDYKDGDISYNPNVPSYSAKYQLSNNDYNVKQLRKRYDIPTKKAPKLLLK
                     GDGDLKGSSIGHKNLEFIFIENKEENIYFTDSINFKPTE"
     gene            2143..>2563
                     /locus_tag="P6L01_002459"
     CDS             2143..>2563
                     /locus_tag="P6L01_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001802535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HDP3031497.1"
                     /translation="MMMHSKKLMLGICLVLLIILIVGYVIMTKINSRSAQIKDTFNQT
                     LKLYPTKNLEDFYDKEGFRDQEFDKRDKGTWIINSGMNIQLKGGALKSREMILYINRN
                     TRTTKGYFIVGEITKDKKGYTHDKDKKYPVKMEHNQII"
BASE COUNT         1080 a          267 c          439 g          777 t
ORIGIN      
        1 atgccgttac taatggattt tatacaacaa cattctatag atccaagcgt tatattttct
       61 aaatttagta attattacga atttttatta aggtataaaa aaatagatgc tttattgacg
      121 gaaaatgaat ctaaaaatct ggttttcttt tcaagacaaa tagcacctgg attgaaaaga
      181 atcgatagtt tagttttaga agaactttta aaaaatgaat taacatatga tgaattaaaa
      241 aataaaatgt tgaacgaagt taaggatata acagaagatg atatagatac ttcattaaga
      301 attttagatt tttcatttta caacgcagga attgaaaaaa tctatggaag tcccattata
      361 gaatgtaatg aaagaatgat aagactaagc gatgcattta ctaatgctct aagtaatcaa
      421 acatttaaaa tatttttaga agatcttatt gaattatcaa agtataacaa tgaaaaatac
      481 caaaaaggta aaaatggact gattttatat aataaatatt caagagaaga ttttagcaaa
      541 atatttaatt ggaataaaaa tggttcgtct gtaatcatgg gatacatgat aagaagccaa
      601 gaaatgccaa tatttatcac ttatgataag catgaggata taagtgatag tacaaaatat
      661 gaagatgaat ttttaagtca agatgaatta aaatggttta ctaaatctaa tagaacgcta
      721 aaatcaaaag aagttcagaa aattttgtcc catagagcaa aaggaattaa aatgtatatt
      781 tttgtacaga aaaaagatga tgatggtata tatttttatt atttaggaac tgctggatat
      841 atagaaggtt cagagaagca agataaaatg ccgaatggtt caaacgtagt aacgatggat
      901 ttagcactcg ataaagctgt aagagatgat atatatcgct atataacaaa ttaatgtaaa
      961 ccgaaaaatg aatgttagta aagatgtatt taaacatcaa ttgtaataag taaagacatt
     1021 gaagtttcag tgttattatt ttaaatataa gcgcaattta tcttaattaa ttagttggtt
     1081 aggccaataa aaattataaa gatatttgcg ctttttaaat aatttgtgat gttgatatgt
     1141 gaaaaataca actaacaatt aaaatccacg aggatttaat cataaaattt cctctagtat
     1201 tattaatatt ggaatttgaa cttaactttg catcaaaaat aaaaatgggg atgtgaaatg
     1261 atgattcatt caagaaagtt gaggctttgg ctgtatttag tattattagc tgtttttata
     1321 ggcgcttgcg gaatgaaaaa ggaagaaagc agtaaggata aacaaattaa agaaaacttc
     1381 aacaaaacgt tgagcttata tccaactaaa aatcttgaag acttttatga taaagaaggt
     1441 tttcgagatg aagagtttga aaaaggagat aaagggactt ggataattca ctctaaaatg
     1501 acaatagaaa caaatggaaa gaatatggaa tccagaggct tggtcctgta tgtagaccgc
     1561 aataccagaa cgacaaaagg tgaatttatt gtgagagaat tatgggaaga taaaaaagga
     1621 tactcacgta gtaaagaaaa agaatatcca gtgaaaatgg aacataataa aattatccca
     1681 actaaaccga tagctgatga taaattaaga aaagaaattg aagactttaa gttttttgta
     1741 caatacggtg actttaaaga tattaatgat tataaagatg gtgacatttc atataatcct
     1801 aatgttccaa gttattccgc aaaatatcaa ttgagtaaca atgactacaa tgtgaagcaa
     1861 cttagaaaga ggtatgatat accaactaag aaagctccga aactattgtt aaaaggtgat
     1921 ggtgatttaa aaggatcatc tataggacac aaaaatttag agtttatatt tatagaaaat
     1981 aaagaagaaa acatctattt tacggatagt attaatttca aacctactga atagggttaa
     2041 tagaagtttg aaatgaaaat ttcataaagt gttcaaaata atttttcgct ttagttgtgt
     2101 aaattaaaga ttgagtcagt attaaaaata taaggtggag aaatgatgat gcattcaaag
     2161 aaattaatgc tcggtatatg tttggtatta ctcattatat tgattgtagg ttatgtcatt
     2221 atgacaaaaa taaatagtcg aagcgcccaa attaaagaca catttaatca aacattaaaa
     2281 ttatatccaa ccaaaaatct tgaagacttt tatgataagg aaggctttcg agatcaagaa
     2341 tttgataaaa gagataaagg gacttggatt attaattctg gaatgaacat ccaattaaaa
     2401 gggggagcct taaaatccag agagatgata ctttacatca atcgtaacac tagaacaacg
     2461 aaaggatatt ttatagtagg ggagataact aaagataaaa agggatatac acatgataaa
     2521 gataaaaaat atcctgtcaa gatggaacat aatcaaatta tcc
//
LOCUS       DAOBBH010000239         2561 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.239, whole genome shotgun sequence.
ACCESSION   DAOBBH010000239 DAOBBH010000000
VERSION     DAOBBH010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.239"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..1612)
                     /gene="bshC"
                     /locus_tag="P6L01_002460"
     CDS             complement(<1..1612)
                     /gene="bshC"
                     /locus_tag="P6L01_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HDP3031498.1"
                     /translation="MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQTSFEKRMS
                     KENNGREAALANVIREYMSDLKLSNEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFH
                     KIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTME
                     MPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALL
                     HETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQQRTQNQGLNAMIQ
                     TDTNVHLFLHDENMRQLVSYDGKHFKLNKTDKTYIKEEIINIAENQPELFSNNVVTRP
                     LMEEWLFNTVAFVGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSK
                     YNIPLEKVLVDGVEGERSKFIREQASHQFIEKVEGMIEQQRRLNKDLLDEVAGNQNNI
                     NLVNKNNEIHIQQYDYLLKRYLLNIERENDISMKQFREIQETLHPMGGLQERIWNPLQ
                     ILNDFGTDVFKPSTYPPLSYTFDHIIIKP"
     gene            1807..2247
                     /locus_tag="P6L01_002461"
     CDS             1807..2247
                     /locus_tag="P6L01_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDP3031499.1"
                     /translation="MSEIKRLEINYKTDELFENFRAFGNKDLYMVNELNGQMIDASSD
                     SPFYGIFVGDQLGARMALLKKGDVEEIYFPDFEDYILLWKLEVLPKYQNRGYASELID
                     FAKSFNMPIKAIGRNDSKDFFLHHGFTDVEAKNIEGHDVLLWKP"
     gene            complement(2356..>2561)
                     /locus_tag="P6L01_002462"
     CDS             complement(2356..>2561)
                     /locus_tag="P6L01_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HDP3031500.1"
                     /translation="NYVFNDIGEDERQHSIIVGDSLTSDILGGINAGIATCWFNFRGF
                     DHNPGIIPDYEINSWKQLNDIVR"
BASE COUNT          823 a          438 c          369 g          931 t
ORIGIN      
        1 aaggttttat aataatatga tcaaaagtgt aagaaagtgg tggataggtg gagggcttga
       61 acacatctgt cccaaaatca ttcaaaattt gaagtggatt ccatattctt tcttgtaatc
      121 ctcccattgg atggagtgtt tcttgaattt ctctaaattg cttcatactg atgtcgtttt
      181 ctctttcaat gtttaaaaga taacgtttta acaaataatc atactgttgt atatgaattt
      241 cattattttt attcacaagg ttaatattat tttgattccc cgccacttca tctaataagt
      301 ctttgtttag acgacgctgt tgttcaatca taccttctac cttttcaata aattgatgtg
      361 atgcttgttc tctaataaac ttacttcttt ctccttcaac accatcgact aacacttttt
      421 ctaatggaat attgtatttc gaaagtaatt tttctatacg gtcatttaaa taagtaattc
      481 taagccttgg catcacgata ggcatttcaa catcaaatag ttcaaataca tcttttagtt
      541 cagcccagta cttaatttca ctcggtcctc caacaaatgc caccgtgtta aataaccatt
      601 cttccattaa tggtcttgtc actacattat tagaaaataa ttcaggttga ttttccgcaa
      661 tatttataat ttcttccttt atatatgtct tatctgtttt atttaattta aaatgcttac
      721 catcatacga aactaattgg cgcatatttt catcatgtaa gaataaatga acatttgtat
      781 ctgtttgtat catcgcattc aagccttgat tttgagtacg ttgttgtgtt gctctaaaag
      841 catcatcaag caactgatgt tttttcaaaa tctttttaaa cataggcgct tccatttttc
      901 ttaacggctc aaactgcgca tctataaata gaacgccata tgctttaaat gtttcatgca
      961 gtagtgcttt aaacatatca gtccacgagt catattggtc aataattctg tcacaaatct
     1021 ccagtagacc ttgtgtatga acagtttctt tcatatgaat gaacatcgtt tttaaagttt
     1081 gtttcaactc agccttatca ggataatatc tagagactgt cgtctctggc atctccattg
     1141 tatgatattt aaccttatgc agcgacccat gattttcgtt ataaacaaat gtatgattca
     1201 cttcatcgaa atcatgatct tctcctgcaa tccaaaaaac tggtactact tgttgcttat
     1261 gtgtatccgt taattcctta gataaagtaa tgattgaaaa tattttatgg aatgtataca
     1321 atggtccccc gaaaagccct gcttgttgtc caccaatcac aacttttgaa ccattagcta
     1381 aatgttgtat gtttaattct tgttcatttg aaagctttaa atcactcata tattcacgaa
     1441 taacattcgc taatgccgct tctcttccat tattttcttt agacatcctt ttttcaaaac
     1501 tagtttgttg agctgcatca tattgaaata atcctgttat tacagggtca ctgctcttta
     1561 tttttggtat aaactgatct ttttcattta aactaactac tttacagtcc atgttttttc
     1621 tccttaagta cgcgattaca aactatagta taaagtctat accggtgatt gacaatttta
     1681 cggcttgaaa atcaatttaa tcatggaaaa tttataatat tcattgtttt acattttcaa
     1741 atcaatgaaa aacacaagtg gtttaatgta taataatagt agtaaacaaa taaggggtag
     1801 ataaatatga gtgaaatcaa acgtcttgaa attaattaca aaactgacga attattcgaa
     1861 aactttagag cgtttggcaa caaagactta tacatggtca atgagttaaa cggtcaaatg
     1921 attgatgcaa gttcagattc accattttat ggcatatttg tcggagatca attaggagct
     1981 agaatggcat tactaaaaaa aggtgatgtc gaagaaatct acttcccaga ttttgaagat
     2041 tatatattat tatggaagtt agaagtatta ccaaaatatc aaaacagagg gtacgcttca
     2101 gaattgattg attttgcaaa gagtttcaat atgccaatta aagccattgg cagaaatgat
     2161 tctaaggatt tctttttaca tcatggattt acagatgtgg aagctaaaaa tatagaggga
     2221 catgatgtct tattgtggaa accataagat aataatattc gacactacga gcatgaaaat
     2281 gcatcttttc gtagtgtcgt ttttacaatt actttcttaa gctaatataa gtaaatcatt
     2341 ttcaaattat ttgtcttaac gtacaatatc atttagttgt ttccatgaat taatttcata
     2401 atcaggtata attcctggat tatgatcaaa tcctctaaaa ttaaaccagc aagtagctat
     2461 acccgcattg attccaccta gaatgtcaga tgttaaagaa tctccaacta taatcgagtg
     2521 ctgtctttca tcctcaccaa tatcattaaa aacataatta a
//
LOCUS       DAOBBH010000240         2531 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.240, whole genome shotgun sequence.
ACCESSION   DAOBBH010000240 DAOBBH010000000
VERSION     DAOBBH010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.240"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            306..1217
                     /gene="rapZ"
                     /locus_tag="P6L01_002463"
     CDS             306..1217
                     /gene="rapZ"
                     /locus_tag="P6L01_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDP3031501.1"
                     /translation="MDNNEKEKSKSELLVVTGLSGAGKSLVIQCLEDMGYFCVDNLPP
                     VLLPKFVELMEQGNPSLRKVAIAIDLRGKELFNSLVAVVDKVKSESDVIIDVMFLEAS
                     TEKLISRYKETRRAHPLMEQGKRSLINAINDEREHLSQIRSIANFVIDTTKLSPKELK
                     ERIRRYYEDEEFETFTINVTSFGFKHGIQMDADLVFDVRFLPNPYYVVDLRPLTGLDK
                     DVYNYVMKWKETEIFFEKLTDLLDFMIPGYKKEGKSQLVIAIGCTGGQHRSVALAERL
                     GNYLNEVFEYNVYVHHRDAHIESGEKK"
     gene            1214..2209
                     /locus_tag="P6L01_002464"
     CDS             1214..2209
                     /locus_tag="P6L01_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDP3031502.1"
                     /translation="MRQIKVVLIGGGTGLSVMARGLREFPIDITAIVTVADNGGSTGK
                     IRDEMDIPAPGDIRNVIAALSDSESVLSQLFQYRFEENQISGHSLGNLLIAGMTNITN
                     DFGHAIKALSKILNIKGRVIPSTNTSVQLNAVMEDGEIVFGETNIPKKHKKIDRVFLE
                     PNDVQPMEEAIDALREADLIVLGPGSLYTSVISNLCVNGISDALIHSDAPKLYVSNVM
                     TQPGETDGYSVKDHIDAIHRQAGQPFIDYVICSTQTFNAQVLKKYEEKHSKPVEVNKA
                     ELEKESINVKTSSNLVEISENHLVRHNTKVLSTMIYDIALELISTIPFVPSDKRK"
     gene            2320..>2531
                     /gene="whiA"
                     /locus_tag="P6L01_002465"
     CDS             2320..>2531
                     /gene="whiA"
                     /locus_tag="P6L01_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDP3031503.1"
                     /translation="MSFASEMKNELTRIDVDEMNAKAELSALIRMNGALSLSNQQFVI
                     NVQTENATTARRIYSLIKRVFNVEVE"
BASE COUNT          921 a          324 c          499 g          787 t
ORIGIN      
        1 tgttggggcc cacaccccaa cttgcattgt ctgtagaaat tggaaatcca atttctctgt
       61 gttggggccc ctgactagag ttgaaaaaag cttgttgcaa gcgcattttc attcagtcaa
      121 ctactagcaa tataatatta tagaccctag gacattgatt tatgtcccaa gctcctttaa
      181 atgatgtata tttttagaaa tttaatctag acatagttgg aaataaatat aaaacatcgt
      241 tgcttaattt tgtcatagaa catttaaatt aacatcatga aattcgtttt ggcggtgaaa
      301 aaataatgga taataatgaa aaagaaaaaa gtaaaagtga actattagtt gtaacaggtt
      361 tatctggagc aggtaaatct ttggttattc aatgtttaga agacatggga tatttttgtg
      421 tagataatct accaccagtg ttattgccta aatttgtaga gttgatggaa caaggaaatc
      481 catccttaag aaaagtggca attgcaattg atttaagagg taaggaacta tttaattcat
      541 tagttgcagt agtggataaa gttaaaagtg aaagtgacgt catcattgat gttatgtttt
      601 tagaagcaag tactgaaaaa ttaatttcaa gatataaaga aacgcgtcgt gcacatcctt
      661 tgatggaaca aggtaaaaga tccttaatta atgcaattaa tgatgagcga gagcatttgt
      721 ctcaaattag aagtatagct aattttgtta tagatactac aaagttatca cctaaagaat
      781 taaaagaacg cattcgtcga tactatgaag atgaagagtt tgaaactttt acaattaatg
      841 tcacaagttt cggttttaaa catggaattc agatggatgc agatttagta tttgatgtac
      901 gatttttacc aaatccatat tatgtagtag atttaagacc tttaacagga ttagataaag
      961 acgtttataa ttatgttatg aaatggaaag agacggagat tttctttgaa aaattaactg
     1021 atttgttaga ttttatgata cccgggtata aaaaagaagg gaaatctcaa ttagtaattg
     1081 ccatcggttg tacgggtgga caacatcgat ctgtagcatt agcagaacga ctaggtaatt
     1141 atctaaatga agtatttgaa tataatgttt atgtgcatca tagggacgca catattgaaa
     1201 gtggcgagaa aaaatgagac aaataaaagt tgtacttatc ggtggtggca ctggcttatc
     1261 agttatggct aggggattaa gagaattccc aattgatatt acggcgattg taacagttgc
     1321 tgataatggt gggagtacag ggaaaatcag agatgaaatg gatataccag caccaggaga
     1381 catcagaaat gtgattgcag ctttaagtga ttctgagtca gttttaagcc aactttttca
     1441 gtatcgcttt gaagaaaatc aaattagcgg tcactcatta ggtaatttat taatcgcagg
     1501 tatgactaat attacgaatg atttcggaca tgccattaaa gcattaagta aaattttaaa
     1561 tattaaaggt agagtcattc catctacaaa tacaagtgtg caattaaatg ctgttatgga
     1621 agatggagaa attgtttttg gagaaacaaa tattcctaaa aaacataaaa aaattgatcg
     1681 tgtgttttta gaacctaacg atgtgcaacc aatggaagaa gcaatcgatg ctttaaggga
     1741 agcagattta atcgttcttg gaccagggtc attatatacg agcgttattt ctaacttatg
     1801 tgtcaatggt atttcagatg cgttaattca ttctgatgcg cctaagctat atgtttctaa
     1861 tgtgatgacg caacctgggg aaacagatgg ttatagcgtg aaagatcata tcgatgcgat
     1921 tcatagacaa gctggacaac cgtttattga ttatgtcatt tgtagtacac aaactttcaa
     1981 tgctcaagtt ttgaaaaaat atgaagaaaa acattctaaa ccagttgaag ttaataaggc
     2041 tgaacttgaa aaagaaagca taaatgtaaa aacatcttca aatttagttg aaatttctga
     2101 aaatcattta gtaagacata atactaaagt gttatcgaca atgatttatg atatagcttt
     2161 agaattaatt agtactattc ctttcgtacc aagtgataaa cgtaaataat atagaacgta
     2221 atcatattat gatatgataa tagagctgtg taaaaaaatg aaaatagaca gtggttctaa
     2281 ggtgaatcat gttttaaata agaaaggaat gactgtacga tgagctttgc atcagaaatg
     2341 aaaaatgaat taactagaat agacgtcgat gaaatgaatg caaaagcaga gctcagtgcg
     2401 ctgattcgaa tgaatggtgc acttagtctt tcaaatcaac aatttgttat aaatgttcaa
     2461 acggaaaatg caacaacggc aagacgtatt tattcgttga ttaaacgtgt ctttaatgtg
     2521 gaagttgaaa t
//
LOCUS       DAOBBH010000241         2527 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.241, whole genome shotgun sequence.
ACCESSION   DAOBBH010000241 DAOBBH010000000
VERSION     DAOBBH010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.241"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(101..1915)
                     /gene="pepF"
                     /locus_tag="P6L01_002466"
     CDS             complement(101..1915)
                     /gene="pepF"
                     /locus_tag="P6L01_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDP3031504.1"
                     /translation="MSQGLPLREDVPVSETWDLVDLFKDDQQYYESIDALVQQANQFH
                     HTYATTLNSIEQINTALAELENILIALDRLSNYAELRLSVDTSNIEAQVLSAKLSTTY
                     GKIVSQLSFVESEILELPEEILQQLEESCPYQHYIKQLIKQKPFQLSASVEQVLATLS
                     PTLNSPYDLYGTTKMLDITFDSFEHDGTTYPVDYATFENDYEDNKDPEFRRKSFKSFS
                     DGIRKYQHTTAATYNMQVQQEKIEADLRGFESVIDYLLHSQEVTRDMFDRQIDMIMRD
                     LAPVMQKYAKLLQRIHGLDNMRFEDLKISVDPDYEPEISIEDSKNYIFGALSVLGDDY
                     TNMLREAYDQRWIDFAQNKGKDTGAFCASPYFTHSYVFISWTGKMAEAFVLAHELGHA
                     GHFTLAQKHQPYLESEASMYFVEAPSTMNEMLMANYLFNTSDNPRFKRWVIGSILSRT
                     YYHNMVTHLLEAAYQREVYHKVDQGESLNAPTLNEIMLNVYKQFFGDAVDMTEGAELT
                     WMRQPHYYMGLYSYTYSAGLTIGTVVSQKIKNEGQPAVDAWLETLKKGGSVSPVELAN
                     IAGVDITTEQPLKSTIQYISDLVDEVEKLTDEIEQANN"
     gene            2120..2464
                     /locus_tag="P6L01_002467"
     CDS             2120..2464
                     /locus_tag="P6L01_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031505.1"
                     /translation="MTLFDMPNYLWITTLIMILLTIFCCLVLNKWFVSAVITFVILGV
                     LAFFIPNFQDIKYQPLLGYAAFLAIMSLLISFLLWYFTRNWRKERKARKLEKQIEKYG
                     YEGAELRRKDKK"
BASE COUNT          822 a          413 c          388 g          904 t
ORIGIN      
        1 tatttctatc attcataaac cctcaaaaaa accaagattc tcacaattag taatgtgaaa
       61 atcttggttt atattgttct actataaatt gtctcgcatc ttagttattt gcttgctcaa
      121 tttcatctgt taatttttca acttcatcga ctaaatcaga aatatattga attgtagatt
      181 taagtggctg ttctgtagta atgtctacac ctgcaatgtt tgcaagttcg acaggtgata
      241 cactaccacc ttttttcaat gtttctaacc aagcatcaac agctggttgg ccttcatttt
      301 taatcttttg agaaacgaca gttccgattg ttaagccagc agaatacgta tacgaatata
      361 atcccatata gtaatgaggt tgacgcatcc atgttaattc agcaccctca gtcatgtcta
      421 ctgcatctcc aaaaaattgt ttataaacat ttagcattat ttcatttaat gtcggcgcat
      481 ttaaagattc accttgatct actttgtgat acacttcacg ttgataagca gcttctaata
      541 aatgggtaac catattatga taatatgttc tagataaaat tgagccaata acccaacgct
      601 taaatcttgg attatcactt gtgttaaata aataattggc catcaacatt tcattcattg
      661 tagaaggggc ttcaacaaag tacattgatg cttctgattc aagatatggt tgatgttttt
      721 gagctaatgt aaaatgacct gcatgaccta attcatgtgc taagacaaat gcttcagcca
      781 ttttaccagt ccaagaaata aacacatatg aatgtgtaaa gtatggactt gcacaaaatg
      841 cgcctgtatc tttaccttta ttttgtgcaa aatcaatcca tcgctgatcg tatgcttcac
      901 gtaacatgtt tgtatagtca tcacctaaaa cacttaacgc accgaaaata taattttttg
      961 agtcttcaat tgaaatctct ggttcataat cagggtctac agaaatcttc aagtcttcaa
     1021 aacgcatgtt atctaatccg tgaatacgtt gtaaaagttt agcatatttc tgcataactg
     1081 gtgccaagtc acgcataatc atatcgattt gacggtcaaa catatcacgc gttacttctt
     1141 gactatgtaa taaataatcg atgactgatt caaatccacg taaatcagct tcaatttttt
     1201 cttgttgtac ttgcatatta tatgtagccg cggtagtatg ctgatatttt cgaatcccat
     1261 cgctaaacga tttgaaactt ttacgtctaa actcaggatc tttattatct tcataatcat
     1321 tttcaaacgt agcatagtcg acagggtacg ttgtaccatc atgttcaaat gaatcgaatg
     1381 taatatctag catttttgtc gtgccgtata aatcgtaagg actgtttagc gtaggtgata
     1441 aagttgctaa tacttgttct accgacgcag ataattggaa tggcttttgt tttattaact
     1501 gtttaatata gtgttgatat ggacatgatt cttctaattg ttgaagtatt tcttctggta
     1561 attcaagtat ttctgactct acaaatgata attggctaac aattttaccg tatgtagtag
     1621 ataatttagc gctcaatact tgtgcctcga tattactagt atctacactt aaacgtagtt
     1681 ctgcataatt acttaagcga tctaaggcaa ttaaaatatt ttctaattca gctaaagcag
     1741 tattaatttg ttcgattgaa tttaatgttg ttgcatatgt atgatgaaat tgatttgctt
     1801 gttgtactag agcgtcaata ctttcataat attgttgatc atctttaaat aagtctacta
     1861 aatcccatgt ttctgaaaca ggaacatctt ctcttaaagg taaaccttga gacatataat
     1921 cactcctttg gtataataca ttcattatac ataattttgt ctcctaaacg ataatgttac
     1981 gatttccatg tcgcaaggaa caatgatttt tctaatagta gatagaatat tgcttgaaac
     2041 tgactgctat tgtttaagtt tttaaaaatt tataataaaa tgtatacaag gaaagtaatg
     2101 aaataaaagg agaatgaata tgacattatt cgatatgcct aattatttat ggatcacaac
     2161 attaataatg attttattaa caatattctg ttgcttagtt ttaaataaat ggtttgtatc
     2221 tgcagtaatt acatttgtta tattaggtgt gcttgcattt tttattccaa attttcaaga
     2281 tataaaatat caaccattat taggatacgc tgcattttta gcgattatga gtttattaat
     2341 tagttttcta ttatggtatt ttactagaaa ttggcgtaaa gaaagaaaag cgcgtaaatt
     2401 agaaaagcaa attgaaaaat acggctatga gggcgctgaa cttcgccgta aagataaaaa
     2461 ataatattcg acaactatat gcacaaaatc atcgactttt aagttacact gcagtaacaa
     2521 ttatttc
//
LOCUS       DAOBBH010000242         2511 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.242, whole genome shotgun sequence.
ACCESSION   DAOBBH010000242 DAOBBH010000000
VERSION     DAOBBH010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.242"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..239)
                     /locus_tag="P6L01_002468"
     CDS             complement(<1..239)
                     /locus_tag="P6L01_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDP3031506.1"
                     /translation="MANIKSAIKRVKTTEKAEARNISQKSAMRTAVKNAKTAVSNNAD
                     NKNELVSLAVKLVDKAAQSNLIHSNKADRIKSQLMT"
     gene            585..2408
                     /gene="lepA"
                     /locus_tag="P6L01_002469"
     CDS             585..2408
                     /gene="lepA"
                     /locus_tag="P6L01_002469"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDP3031507.1"
                     /translation="MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRD
                     MQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
                     GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISS
                     IRIVDGVVKAGDKIRMMATGKEFEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDD
                     SRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLREALEKLQLNDASLE
                     FEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCILRDGSEVT
                     VDNPAQMPDRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVN
                     IVYELPLAEVVFDFFDQLKSNTKGYASFDYEFIENKESNLVKMDILLNGDKVDALSFI
                     VHRDFAYERGKALVEKLKTLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVLAKCYGG
                     DISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLAVLKMDDE"
BASE COUNT          893 a          326 c          485 g          807 t
ORIGIN      
        1 gtcattaatt gtgatttaat acggtcagct ttgtttgaat gtattaaatt actttgagca
       61 gctttgtcta ctaacttaac tgctaagctt actaattcat ttttattatc agcgttattt
      121 gaaacagctg ttttagcgtt tttaactgct gtacgcattg cactcttttg tgaaatgttg
      181 cgtgcttcag ctttttcagt tgtttttaca cgtttaattg cagatttgat atttgccatt
      241 tctgtcacct cctaaaagtg agttcatctt atcaaaattt tatttgatta caacaagaaa
      301 tattttaaca aaagtctaac cgttgtgcaa tcatttattt gatggggtta gcttaaaatt
      361 ttgcagattt aataaattgt gttggaaaca caatcaatac gttttataat tataagtacg
      421 taattaacat attaacatat cgcaagtatg tatttaaata aagattgtta taatttcaaa
      481 gttcatccaa gattatggcg tttgcattta cctattaaaa acgttattat atcaaagatg
      541 cgaaagataa tacgggttta ttttatgaaa gtgagaagga taaaatggat aatgagcaac
      601 gcttaaaaag aagagagaat ataaggaatt tctcgattat agcacatatt gaccacggaa
      661 aatctacatt ggctgataga attttagaaa ataccaaatc agttgaaaca agagatatgc
      721 aagatcagtt actagattca atggatttag aaagagaacg tggtattaca atcaaattaa
      781 acgcagttcg tttaaagtac gaagctaaag atggaaatac ttatacattc catttaatcg
      841 atacgcctgg acacgtcgat tttacatatg aagtgtcacg ttctttggca gcttgtgagg
      901 gtgcgatttt agtagtagat gcggctcaag gtatcgaagc acaaacatta gcaaatgttt
      961 atttggcatt agataatgag ttagagttat tgcctgttat taacaaaatt gatttacctg
     1021 ctgcagaacc tgaacgcgtg aaacaagaaa ttgaagatat gataggttta gaccaagacg
     1081 atgttgtttt agcaagtgct aaatctaaca ttggaattga agagatacta gagaaaatag
     1141 ttgaagttgt gccagctcca gatggtgacc cagaagcacc actaaaagca ttaatatttg
     1201 attctgagta tgatccatat agaggggtaa tttcatcgat aagaattgta gacggtgttg
     1261 ttaaagctgg agataaaatt cgaatgatgg ccactggtaa agagttcgaa gtaacagaag
     1321 ttggaattaa tacacctaag cagcttccag ttgatgaatt aacagttggt gatgttggtt
     1381 atattattgc aagtattaaa aatgttgatg attctagggt tggtgacacc atcacattag
     1441 ctagtagacc tgcatcagaa ccattgcaag gttataagaa aatgaatcca atggtatatt
     1501 gcggactgtt cccaatagat aacaaaaatt ataatgattt aagagaagca ttagaaaaat
     1561 tacaattgaa tgatgcatca ttagaatttg agcctgaatc gtcacaagca ttaggttttg
     1621 gttatagaac tggtttctta ggtatgttac acatggaaat aattcaagaa agaattgaaa
     1681 gagaatttgg cattgaatta attgcaacag caccatctgt aatttatcaa tgtattttaa
     1741 gggacggttc agaagtgacg gttgataacc cagcacaaat gccagatcgt gataaaattg
     1801 ataaaatatt tgagccatat gttcgtgcaa ctatgatggt tccaaatgac tatgtaggtg
     1861 cagtaatgga attatgtcaa cgtaaacgtg gacaatttat aaatatggac tatttagatg
     1921 atattcgtgt aaatattgtt tatgaattac ctttagctga agttgtattt gatttcttcg
     1981 atcaacttaa atctaatact aaaggatatg catcatttga ttatgaattc atcgaaaata
     2041 aagaaagtaa tttagttaag atggatattt tattaaacgg tgataaagtg gatgcgctaa
     2101 gcttcatagt tcatagagat tttgcatatg aacgtggtaa agcattagtt gaaaaactta
     2161 aaacgttaat tccaagacag caatttgaag tacctgtaca ggctgcaata ggacaaaaaa
     2221 ttgtagcgcg tacaaatatt aaatcaatgg gtaaaaacgt tttagctaaa tgttatggcg
     2281 gtgacataag ccgtaaacgt aaattacttg aaaaacaaaa agcaggtaaa gctaagatga
     2341 aagcagttgg taatgttgaa attccacagg atgctttctt ggctgtattg aaaatggatg
     2401 acgaataagt ttttaaaaaa tttattaaca atttacaatg aataaagttt aataactaaa
     2461 aagagggagc ctaggataaa ttaacgtcct gggctttaca atgttatatt g
//
LOCUS       DAOBBH010000243         2467 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.243, whole genome shotgun sequence.
ACCESSION   DAOBBH010000243 DAOBBH010000000
VERSION     DAOBBH010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.243"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(64..1119)
                     /gene="saeS"
                     /locus_tag="P6L01_002470"
     CDS             complement(64..1119)
                     /gene="saeS"
                     /locus_tag="P6L01_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SaeS"
                     /protein_id="HDP3031508.1"
                     /translation="MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLT
                     TIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSF
                     NKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIK
                     ESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHEN
                     RTLEVNFCSEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENKDQDTIDIA
                     ISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDI
                     DVGTTMTVTLHKLDITS"
     gene            complement(1119..1805)
                     /locus_tag="P6L01_002471"
     CDS             complement(1119..1805)
                     /locus_tag="P6L01_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDP3031509.1"
                     /translation="MTHLLIVDDEQDIVDICQTYFEYEGYKVTTTTSGKEAISLLSND
                     IDIMVLDIMMPEVNGYDIVKEMKRQKLDIPFIYLTAKTQEHDTIYALTLGADDYVKKP
                     FSPRELVLRINNLLTRMKKYHHQPVEQLSFDELTLINLSKVVTVNGHEVPMRIKEFEL
                     LWYLASRENEVISKSELLEKVWGYDYYEDANTVNVHIHRIREKLEKESFTTYTITTVW
                     GLGYKFERSR"
     gene            complement(1780..2253)
                     /locus_tag="P6L01_002472"
     CDS             complement(1780..2253)
                     /locus_tag="P6L01_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDP3031510.1"
                     /translation="MNKLLLLVTFIIRVGSGIVMLMQGYEKLTGGFTLKGLVPVIANN
                     TDSPEWYKWFFANIVAHTTSLFDIVVPLGEIAIGLGLIFGVFAYAASFFGAFVMINYI
                     LADMIFTYPLQLTFFILLLMSHSLLKQISLKEIINYFRGRKNRGEKIDDPLTDRG"
BASE COUNT          773 a          390 c          387 g          917 t
ORIGIN      
        1 agaagccctc attaatggga gcttcttttt agtctttgca ttttatttta taaataaatc
       61 ggattatgac gtaatgtcta atttgtgtaa tgttacagtc atcgtagttc ctacatctat
      121 atcactgctt acactgattt ttgcgttatt ttgttgcgcg agttcattag ctatatataa
      181 gcctaatcca gaaccacccg tttttgtatt acgagagttt tctactctga atgtacgttc
      241 gaatatacgt tcttgtagtt ctggtataat gccaatacct tcatcgctaa tagcaatgtc
      301 gatagtatct tgatctttgt tttcactaat attaatatca atgcgactac caacatttga
      361 aaattttagc gcattatcaa gtaagtttgt taaaatacgc tcaagtggcg ttcgatattg
      421 ataaaatgca tcaatttcgc tacagaaatt cacttctaat gtgcggtttt catgtttgat
      481 acgttgctca tatggttgca atattgatac aagtaattgg tctagttgta ttaattctgg
      541 gggatatgtt ttacctgtat ttaaagtgat aatatgagtc atatcatcaa ataatgttga
      601 taatctgttt gcttgtttaa ttaatatgtc gtatgactct ttaatctcat gatcctttgt
      661 gattatacca tcacgtagtc cttcagaata tgaaataatg cttgctaaag gtgtttttaa
      721 atcatgggct aagttttgaa tcagttctgt tttttcttgt tgttcggatt taatttgatt
      781 catttgttgc gtaatttcag aagccatttt attaaaagat tgatttaatt cataaatttc
      841 ttttggtgaa ttaaacgttt tatcattgct tgcgtaattt ccgttagcaa attgcttagt
      901 ttttatatta aactgcttaa ttttttgtat aagtggatta ataaaaatac tacatattaa
      961 taaggttaaa cagcttgtaa ttattgtcgt taaggtcaaa gttagtgtca tatggccgtt
     1021 aaaccacatt aaaatatatg caattgctaa aatagttgaa gttaatagta tactcgatac
     1081 gacgccaata atgatttgac ttctaattga taacaccatt atcggctcct ttcaaattta
     1141 tatcctaatc cccatacagt tgtgatggta tatgttgtaa agctctcttt ttctaatttt
     1201 tctctaatac ggtgtatatg gacattcacg gtattagcat cttcgtaata gtcatatccc
     1261 caaacttttt caagtaattc tgatttagaa ataacttcat tttctctaga agctaaatac
     1321 cacaataact caaattcctt aatacgcata gggacttcgt gaccatttac agtcacaact
     1381 ttacttaagt taataagtgt taattcatca aacgacagtt gttcaactgg ttgatgatgg
     1441 tatttcttca ttcttgtaag taaattatta atacgtaaaa cgagttccct tggactaaat
     1501 ggttttttga catagtcatc tgcacctaaa gttaaggcgt aaatggtatc atgttcttgt
     1561 gttttggcag ttaaatagat aaaggggata tctaattttt gccttttcat ttctttgaca
     1621 atgtcgtaac cattaacttc tggcatcatg atatcaagta ccatgatatc aatatcattt
     1681 gatagtaaag aaattgcttc tttaccgcta gttgtcgttg ttactttgta accttcatat
     1741 tcaaaatagg tttgacaaat gtctacaatg tcttgttcat catccacgat cagtaagtgg
     1801 gtcatctatt ttttcacctc tgttcttacg acctctaaag taattaatga tttctttaag
     1861 tgaaatctgt tttaacaatg agtgactcat tagtaaaagg ataaagaaag ttaattgaag
     1921 aggatacgta aatatcatat ctgctaagat ataatttatc ataacaaagg ctccaaagaa
     1981 actagcagca tatgcaaaaa ctccaaaaat taaacctaat ccaattgcaa tctctccgag
     2041 tgggacaaca atatcaaata atgacgtcgt atgtgcaact atatttgcga aaaaccactt
     2101 ataccactct ggtgaatcag tattgttagc gatgactggt actaaacctt tcagcgtaaa
     2161 tccgcccgtt aatttttcgt agccttgcat taacataaca atacctgaac ccacacgaat
     2221 gataaatgta acgagtagca ataatttatt catgatgttc acattctttc tatttattgt
     2281 gtgtaattta tataaacata agattaagca acataatgcg atttgtagtg ttatgtgaac
     2341 aggaagtgtt tgagctcatg ggttgcttaa gctaaacaaa cctcaagctc taaaaaaatt
     2401 ttacttatgt gctctacaat cttatgggat ttaatagttg tatatatatt gaaaaaagga
     2461 aagtatg
//
LOCUS       DAOBBH010000244         2467 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.244, whole genome shotgun sequence.
ACCESSION   DAOBBH010000244 DAOBBH010000000
VERSION     DAOBBH010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.244"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(198..1181)
                     /gene="lukD"
                     /locus_tag="P6L01_002473"
     CDS             complement(198..1181)
                     /gene="lukD"
                     /locus_tag="P6L01_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit D"
                     /protein_id="HDP3031511.1"
                     /translation="MKMKKLVKSSVASSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDNDKLNISQILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQF
                     YWGGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNG
                     SKSFSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNEL
                     FLGGRQSSSNAGQNFLPTHQMPLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMD
                     RYTNQWNRLHWIGNNYKNQNTVTFTSTYEVDWQNHTVKLIGTDSKETNPGV"
     gene            complement(1183..2118)
                     /gene="lukE"
                     /locus_tag="P6L01_002474"
     CDS             complement(1183..2118)
                     /gene="lukE"
                     /locus_tag="P6L01_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114304764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit E"
                     /protein_id="HDP3031512.1"
                     /translation="MFKKKMLAATLSVGLIAPLASPIQESRANTNIENIGDGAEVIKR
                     TEDVSSKKWGVTQNVQFDFVKDKKYNKDALIVKMQGFINSRTSFSDVKGSGYELTKRM
                     IWPFQYNIGLTTKDPNVSLINYLPKNKIETTDVGQTLGYNIGGNFQSAPSIGGNGSFN
                     YSKTISYTQKSYVSEVDKQNSKSVKWGVKANEFVTPDGKKSAHDRYLFVQSPNGPTGS
                     AREYFAPDNQLPPLVQSGFNPSFITTLSHEKGSSDTSEFEISYGRNLDITYATLFPRT
                     GIYAERKHNAFVNRNFVVRYEVNWKTHEIKVKGHN"
BASE COUNT          767 a          410 c          327 g          963 t
ORIGIN      
        1 tttaataatt ttttatatgt tggaagtttc taaataattc tactttcgta aatttagcac
       61 cctgctattt agcaaagtaa tctacatttc cctaactaaa aattatcatg tgtgtaaata
      121 aaaattatgt tagttcttta attccgtttg ataattgaaa taattgcact ttgttgatta
      181 tttaatcata tacaattcta tactccagga ttagtttctt tagaatccgt accgattaat
      241 ttaacagtat ggttttgcca gtcaacttca taagtagatg taaacgttac tgtattttga
      301 tttttgtagt tattaccaat ccagtgtagt cgattccatt gattagtata tctatccatt
      361 tctctttggt aagttacttt gattttagat ttttttgtat cattttgttt atgagaaagt
      421 acgcttataa attctgggtt aaagttacca cgcgccaata aaggcatttg atgtgttggc
      481 aagaaatttt gtccagcatt tgaactactt tgtctaccac ctaaaaacag ttcattacca
      541 tatgttgggt cataactatc tctaccatat ggtccccaac cattattcat aattttgtgc
      601 gcctcaacac cccagccaat tgatttatga tttgtttttc tatcaatcgt agttctgtaa
      661 ctttcttgtt tataatttat tgtttctgaa aatgattttg atccattcaa tccacctgat
      721 aagccattag atatattaat atctccgcca taagaataac ctaatgtttg ttgaacttgg
      781 aattcttcat tttgattttt aggtgcatag tcaacaacat ttacagcatc atttgattct
      841 gaactaaccg aaacattata cttaccgccc caataaaact gtgagtaatt gtaatctttt
      901 ggattaggct ttttataacc tgaattaatg tttccggctg ccttaagtac taatgtatct
      961 ttgtcataac tcttatcctt aatgaaatta aacgttaaaa tttgagaaat attcaattta
     1021 tcattatcag atgttgctgt tgttttgtat aaagtgattt tgtcatctac ttttttctcg
     1081 cttacaggtg tgatatgttg agctgcatca actgtattcg atagcaaaag cagtgcaatt
     1141 gatgaagcaa ctgatgattt gactaatttt ttcattttca tattaattat gtcctttcac
     1201 tttaatttcg tgtgttttcc aattaacttc atatctaact acaaagtttc tatttacaaa
     1261 tgcattatgc tttctttctg cgtaaatacc agttctaggg aataaagtcg catatgtaat
     1321 atctaagttt ctaccatatg aaatttcaaa ttcactcgta tcacttgaac ctttttcatg
     1381 tgatagtgta gtgataaatg atggattaaa gccactttga actaaaggtg gcaattgatt
     1441 atcaggagca aaatattctc ttgctgaacc tgttggacca tttggacttt gtacgaataa
     1501 atatctatca tgcgcagatt ttttaccatc aggcgtaaca aattcgtttg ctttaacacc
     1561 ccatttaact gattttgagt tttgcttgtc tacttcactg acataactct tttgggtata
     1621 actaattgtt ttagaataat taaatgagcc attgccacct atagatggtg ctgactggaa
     1681 attacctcca atgttatatc ctaatgtttg accaacatca gtagtttcta ttttgttttt
     1741 aggaaggtaa ttgattaagc taacatttgg atctttagtc gtcagtccta tattatattg
     1801 gaatggccaa atcattcgtt tagtcaattc atatccacta cccttcacat ctgaaaatga
     1861 agttctggaa ttaataaaac cttgcatttt aacaattaaa gcatctttgt tatatttttt
     1921 atcttttaca aagtcgaatt gaacattttg agtaacgccc catttcttac tacttacatc
     1981 ctccgtacgt ttgattactt cagcaccatc accaatattc tctatattag tattagctct
     2041 agattcttga atcggagatg ctaaaggcgc aatcagtcct actgacaaag ttgcagctaa
     2101 catttttttc ttaaacataa gtttcacttt ctttctatat aaatttttaa tacaaattta
     2161 ttctagatta tttcacttat ttataaaatt aactgttcat taaaaaaata aaatgctata
     2221 aaatattaaa aatgatacaa tttattcatt tatgatgctg aaaaccggtc attttggcgc
     2281 aaccttcatt acatttaaat agttcccttt atactcgaac aatatgataa aattgccgat
     2341 gctcaacttt ttcgctataa aaatcaaaac gaactttaat caaatcttaa aaaataatgt
     2401 ttgtattatc aaaaataatc tataattgaa tttaaaatta actgattatt aactattgtc
     2461 cgttaaa
//
LOCUS       DAOBBH010000245         2435 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.245, whole genome shotgun sequence.
ACCESSION   DAOBBH010000245 DAOBBH010000000
VERSION     DAOBBH010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.245"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(367..1080)
                     /locus_tag="P6L01_002475"
     CDS             complement(367..1080)
                     /locus_tag="P6L01_002475"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDP3031513.1"
                     /translation="MAKLNVEVFADGADIEEMKAAYKNKQVDGFTTNPSLMAKAGVTD
                     YKAFAEEAVKEIPDASISFEVFADDLETMEKEAAILKQYGENVFVKIPIVNTKGESTI
                     PLIKKLSADNVRLNVTAVYTIEQVKEITEAVTEGVPTYVSVFAGRIADTGVDPLPLMK
                     EAVKVTHSKDGVKLLWASCRELFNVIQADEIGADIITCPADVVKKVNTNLGRDINELS
                     VDTVKGFAKDIQSSGLSIL"
     gene            1340..1642
                     /locus_tag="P6L01_002476"
     CDS             1340..1642
                     /locus_tag="P6L01_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031514.1"
                     /translation="MSRSKKYFYLSSLMIILSFFFNTNNVFLSGFFNSFIKLILFCSV
                     INSIVLILSIIFADRSIKSLKPDADWIRIASKSLPWIILIVILVHIFSIVRTFGFI"
     gene            1898..2263
                     /gene="crcB"
                     /locus_tag="P6L01_002477"
     CDS             1898..2263
                     /gene="crcB"
                     /locus_tag="P6L01_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDP3031515.1"
                     /translation="MQYVYIFIGGALGALLRYLISFLNTDGGFPIGTLIANLTGAFVM
                     GLLTALTIAFFSNHPTLKKAITTGFLGALTTFSTFQLELIHMFDHQQFITLLLYAVTS
                     YVFGILLCYVGIKLGGGLS"
     gene            2260..>2435
                     /locus_tag="P6L01_002478"
     CDS             2260..>2435
                     /locus_tag="P6L01_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HDP3031516.1"
                     /translation="MISIILVMIGGGFGAIARSAITDYFNHKFTSKLPIATLIVNLVG
                     SFLIGLTIGLSISIS"
BASE COUNT          757 a          400 c          332 g          946 t
ORIGIN      
        1 taaactcaaa aatacgtgtc atcaagtgca aaataattta gaactaatac aaacataaaa
       61 acttttgctt tacgcttaaa atcaaaagtc acatacctag atataagtac aacttgtata
      121 ttttgtactc taataagtat gtggcttaaa taatatttaa ttcttgagtt gcatatagta
      181 attgttttta ttcattttct aaaatatgcg agactaattt ccttttctac tcctgttgaa
      241 tcaatttaga cttttttata actcgggctt ttacttagct gaattttgac ttcttcttca
      301 ttaataacta tgatggaaaa acccccataa gcacaatgcc tatgagggtt taaatatgtt
      361 tataaattat agaatagaaa gacctgaaga ttgaatatct ttcgcaaagc ctttaactgt
      421 atctactgat aattcgttaa tatcgcgacc taagtttgta ttcacttttt tcacaacatc
      481 tgcagggcat gtaataatat ctgcaccaat ttcatcagct tgaatcacat tgaataattc
      541 gcggcaactt gcccataata atttaacgcc gtctttacta tgcgtaactt ttacagcctc
      601 tttcattaat ggtaatggat ctacgcctgt atctgcaata cgtcctgcaa atactgaaac
      661 atatgttggc acaccttcag ttactgcttc agttatttct ttaacttgtt caattgtgta
      721 aacagccgta acgtttaatc tcacattgtc agctgaaagt tttttaatta aaggaatcgt
      781 tgattcacct tttgtattta caataggaat tttaacaaat acattttcgc catattgttt
      841 taaaattgct gcttcttttt ccatagtttc taaatcgtct gcaaatactt caaatgaaat
      901 tgaagcatct ggaatttctt tcacagcttc ttcagcaaaa gctttgtaat ctgttacgcc
      961 cgctttcgcc attaaactag gatttgttgt aaaaccatcc acttgtttgt ttttataagc
     1021 tgctttcatt tcttcaatat ctgcaccgtc cgcaaatact tctacattta gtttagccat
     1081 ataatatagc ctccttgatt cttattaaaa ttttaacaac atctgcatgt ctttttctta
     1141 caaccatttg taaaaaatga tttttatttc tttgttatat aaaacattta tatcacgctt
     1201 ttataatata tagctaattt tttgcctata ttttttaaaa atgactttag caaacattgt
     1261 gtctacaagt taataaaact attgttttat gattgtttat gatacgatat gtaaatgata
     1321 atcatccagg aggtctagca tgtctcgttc aaaaaaatac ttttacttat ctagcttaat
     1381 gattatttta agctttttct ttaatacaaa taacgttttc ctaagtggat tttttaattc
     1441 ttttattaaa ttaatacttt tctgcagtgt tattaactca attgtactaa ttttgtctat
     1501 aatttttgca gatcgttcaa ttaaatcact aaagcctgat gcagattgga ttagaattgc
     1561 gagtaaaagt ttgccttgga ttattctaat tgttatttta gtacatatct tttcaattgt
     1621 tcgtacattc ggttttattt aaaaaagata aaatgtcaat gtagcgttaa tacaaaacaa
     1681 tacaatgtat catgctataa tgagtaaaac aatttgatga cgttgttgcg tataaaaaat
     1741 attagatttt cgaaatcata actatgcatc taatcgctat agttatacaa caagatataa
     1801 catataatga ggtttgataa tgcatcgaca atttttgtcg tcgtgttgcc aaaacctctt
     1861 ttttaaattc aaactacttc ttttcgaggt gaaccaaatg caatatgtat atatttttat
     1921 cggtggtgct ttaggcgctt tattacgtta cctcatttct tttctgaata ctgacggagg
     1981 ttttccaatc ggaacactga tagccaattt gactggtgcc tttgtaatgg gattgctaac
     2041 agccttaaca attgcatttt tttcaaacca tccgacccta aaaaaagcta ttacgactgg
     2101 ttttcttggt gctttaacga ctttttcaac atttcaatta gaattaatac atatgtttga
     2161 tcatcaacaa tttataactt tactactata tgctgtaaca agttatgtct ttggtatttt
     2221 gttatgttac gtcggtataa aactaggtgg tggtttatca tgatatcaat cattttagtc
     2281 atgattggcg gcggttttgg cgcaattgct agaagtgcca ttactgatta ttttaatcat
     2341 aaatttactt caaagttacc tatcgcaaca ttgatagtaa atctagttgg tagtttttta
     2401 attggattaa ctataggcct atcaatttca atctc
//
LOCUS       DAOBBH010000246         2403 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.246, whole genome shotgun sequence.
ACCESSION   DAOBBH010000246 DAOBBH010000000
VERSION     DAOBBH010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.246"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            151..543
                     /locus_tag="P6L01_002479"
     CDS             151..543
                     /locus_tag="P6L01_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HDP3031517.1"
                     /translation="MKKVINVVGAIIFSDNKILCAQRSEEMSLPLMWEFPGGKIEKNE
                     TEKEALIREIREEMKCDLIVGDKVITTEHEYDFGIVKLTTYKCTLNKELPTLTEHKSI
                     EWLSINELDKLNWAPADIPAVNKIMTEG"
     gene            545..>2403
                     /locus_tag="P6L01_002480"
     CDS             545..>2403
                     /locus_tag="P6L01_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDP3031518.1"
                     /translation="MSRLLNDFNQSLKKGFIDKDISHKGNYTPKLLVNNKNEKVLSTI
                     IDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGEL
                     LKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKN
                     GDLVDSVKNEFELLWQKSTPLTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKS
                     VEIVPNLMQAEALRSLKAIRDKTKDKALIISATGTGKTILCALDVREVNPNKFLFIVH
                     NEGILNRAKEEFKKVLPIKNDSDFGLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDEN
                     EFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFELFDYNIAYEIR
                     LQAALESDILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKTDYYGYSGE
                     ILQGLIFVSSKKEAYDLADKLSSKGIKSVALTGDDSVNYRQIVIEKLKEGKINYIITV
                     DLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKSANKEYVTVIDFIGNYKTNYL
                     IPIALSGDQSQNKDNYKKFLTNNDSINGVSTINFEEVAKKQIYNSLDAVSLNQ"
BASE COUNT          933 a          254 c          440 g          776 t
ORIGIN      
        1 gatgtgtatg aaaatgcatg gagtagatgt aagagtgata tatcatgcag gaaattgtaa
       61 aatattaatt tttaatattg atttatatat agttttgtaa tttttgatag aataacaatt
      121 aagatgattt taaattgaag ggtgagtctt atgaaaaaag taatcaatgt agtaggagct
      181 attatttttt ctgataacaa aattctttgt gcacagagaa gtgaagaaat gagtctacct
      241 ttaatgtggg aatttcctgg cggtaagatt gaaaagaatg aaactgaaaa agaagctttg
      301 attagagaaa ttagagaaga aatgaaatgt gatctaattg ttggagacaa agtcataact
      361 acagaacatg aatatgattt tggcattgtt aagttaacaa catacaaatg tactttaaac
      421 aaagagttac caactttaac tgaacataag agtattgaat ggttgtcaat aaacgaactt
      481 gataaattaa attgggcccc agcggatata ccagccgtta ataaaattat gactgaggga
      541 taatatgagt agattactaa atgatttcaa tcaatcttta aaaaaaggtt ttatagacaa
      601 agatatttct cataaaggga attatacacc gaagctttta gtaaataata aaaatgaaaa
      661 agtcctttca acaattatag atgaacttca aaaatgtgaa acgttctatt tttctgtagc
      721 ttttataaca gaaagcggat tagccagctt aaaggctcaa ctattagatt taagcaataa
      781 gggtgttaaa gggaaaatat taacatctaa ttacttagga tttaatagtc ctaaaatgta
      841 tggagaatta cttaaattaa aaaatgtaga ggtaagatta actgatattg ctggattcca
      901 tgccaaagga tatatttttg aacataagga ttatagttct atggtaatag gaagctctaa
      961 tttaacatct aatgcgttaa aggttaatta tgagcataat gttttactgt ctactatgaa
     1021 aaatggtgat ttagttgata gtgtaaaaaa tgaatttgaa ttgttatggc aaaagagtac
     1081 cccactgact gagcaatgga ttaattcata taaagaatca tttgagtacc gttccttaga
     1141 aaaattagcg gaggttgaac aaactcaaat gctgctagct gataaggtga aaaaatcagt
     1201 ggaaattgta cccaatttaa tgcaagcaga agcattaagg tcattaaaag ctattaggga
     1261 taaaaccaaa gataaggcgt taataatatc tgcaactggt acaggtaaaa cgattttatg
     1321 tgcattagat gttagagaag taaaccctaa taaattttta tttattgttc ataatgaggg
     1381 gattttaaat agagctaagg aagaatttaa aaaagtatta cctataaaaa atgatagtga
     1441 ttttggattg ttgacaggaa aacatagaga tgtcgatgcc aaatatttat ttgcaaccat
     1501 tcaaacatta tctagagatg ataattttaa gcaatttgat gaaaatgaat ttgattatat
     1561 tgtttttgat gaagcacatc gttctgctgc atctacttat caacgagtat ttaactactt
     1621 caaacctaag ttcatgctgg gaatgactgc tacaccagaa agatcagatg aattaagtat
     1681 atttgaactg tttgattata atattgcata tgaaataagg ttacaagcag cgttagaaag
     1741 tgatatttta tgtccatttc attattttgg tgtgactgat tatgtacatc aaggtattaa
     1801 agaagatgat gtaactaaac tgagatattt aacttctgat gaaagagtta attatattat
     1861 tcaaaagaca gattactatg gatattcagg tgaaatttta caaggattaa tttttgtgag
     1921 tagcaaaaaa gaagcttatg atttagcgga taaactaagt tcaaaaggga ttaaaagtgt
     1981 tgctttaaca ggagatgata gtgttaatta tcgccaaatt gtaatagaaa aattaaaaga
     2041 aggtaagatt aactatatta taactgtaga tctttttaac gaaggtattg atattccaga
     2101 agtcaatcaa gttgtaatgt tgagaccaac agagtctagt attatattca ttcagcaact
     2161 tggtagagga ttaagaaaaa gtgcaaataa agagtatgtt actgtaattg attttattgg
     2221 taattataag accaattatt taattccgat tgcgctttct ggggatcaat cgcaaaataa
     2281 agataattat aagaagtttt taacgaataa cgattcgatt aatggagtat ctacaattaa
     2341 ttttgaagaa gttgctaaaa aacagattta caattcattg gatgcagtat cattgaatca
     2401 aaa
//
LOCUS       DAOBBH010000247         2390 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.247, whole genome shotgun sequence.
ACCESSION   DAOBBH010000247 DAOBBH010000000
VERSION     DAOBBH010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.247"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            182..1123
                     /locus_tag="P6L01_002481"
     CDS             182..1123
                     /locus_tag="P6L01_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDP3031519.1"
                     /translation="MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHW
                     ADDDQDTSDLAYEASVKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGK
                     VASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDG
                     AGAVIIGEVSEGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDAS
                     TRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPL
                     SIDQELKNGKLKDDDTIVLVGFGGGLTWGAMTIKWGK"
     gene            1135..2379
                     /gene="fabF"
                     /locus_tag="P6L01_002482"
     CDS             1135..2379
                     /gene="fabF"
                     /locus_tag="P6L01_002482"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDP3031520.1"
                     /translation="MSQNKRVVITGMGALSPIGNDVKTTWENALKGVNGIDKITRIDT
                     EPYSVHLAGELKNFNIEDHIDKKEARRMDRFTQYAIVAAREAVKDAQLDINDNTADRI
                     GVWIGSGIGGMETFEIAHKQLMDKGPRRVSPFFVPMLIPDMATGQVSIDLGAKGPNGA
                     TVTACATGTNSIGEAFKIVQRGDADAMITGGTEAPITHMAIAGFSASRALSTNDDIET
                     ACRPFQEGRDGFVMGEGAGILVIESLESAQARGANIYAEIVGYGTTGDAYHITAPAPE
                     GEGGSRAMQAAMDDAGIEPKDVQYLNAHGTSTPVGDLNEVKAIKNTFGEAAKHLKVSS
                     TKSMTGHLLGATGGIEAIFSALSIKDSKVAPTIHAVTPDPECDLDIVPNEAQDLDITY
                     AMSNSLGFGGHNAVLVFKKFEA"
BASE COUNT          821 a          361 c          514 g          694 t
ORIGIN      
        1 tatctgaatg gctattcaaa tttaagcgca ttaaaaccaa tttcatactg aaatttgacg
       61 ataataaagc attaaaattt tattaactag tcaatattcc tacctctgac ttgagtttaa
      121 aaagtaatct atgttaaatt aatacctggt attaaaaatt ttattaagaa ggtgttcaac
      181 tatgaacgtg ggtattaaag gttttggtgc atatgcacca gaaaagatta ttgacaatgc
      241 ctattttgag caatttttag atacatctga tgaatggatt tctaagatga ctggaattaa
      301 agaaagacat tgggcagatg acgatcaaga tacttcagat ttagcatatg aagcaagtgt
      361 aaaagcaatc gctgacgctg gtattcagcc tgaagatata gatatgataa ttgttgccac
      421 agcaactgga gatatgccat ttccaactgt cgcaaatatg ttgcaagaac gtttagggac
      481 gggcaaagtt gcctctatgg atcaacttgc agcatgttct ggatttatgt attcaatgat
      541 tacagctaaa caatatgttc aatctggaga ttatcataat attttagttg tcggtgcaga
      601 taaattatct aaaataacag atttaactga ccgttctact gcagttctat ttggagatgg
      661 tgcaggtgcg gttatcatcg gtgaagtttc agaaggcaga ggtattataa gttatgaaat
      721 gggttctgat ggcactggtg gtaaacattt atatttagat aaagatactg gtaaactgaa
      781 aatgaatggt cgagaagtat ttaaatttgc tgttagaatt atgggtgatg catcaacacg
      841 tgtagttgaa aaagcgaatt taacatcaga tgatatagat ttatttattc ctcatcaagc
      901 taatattaga attatggaat cagctagaga acgcttaggt atttcaaaag acaaaatgag
      961 tgtttctgta aataaatatg gaaatacttc agctgcgtca atacctttaa gtatcgatca
     1021 agaattaaaa aatggtaaac tcaaagatga tgatacaatt gttcttgtcg gattcggtgg
     1081 cggcctaact tggggcgcaa tgacaataaa atggggaaaa taggaggata acgaatgagt
     1141 caaaataaaa gagtagttat tacaggtatg ggagcccttt ctccaatcgg taatgatgtc
     1201 aaaacaacat gggagaatgc tctaaaaggc gtaaatggta tcgataaaat tacacgtatc
     1261 gatactgaac cttatagcgt tcacttagca ggagaactta aaaactttaa tattgaagat
     1321 catatcgaca aaaaagaagc gcgtcgtatg gatagattta ctcaatatgc aattgtagca
     1381 gctagagagg ctgttaaaga tgcgcaatta gatatcaatg ataatactgc agatcgaatc
     1441 ggtgtatgga ttggttctgg tatcggtggt atggaaacat ttgaaattgc acataaacaa
     1501 ttaatggata aaggcccaag acgtgtgagt ccatttttcg taccaatgtt aattcctgat
     1561 atggcaactg ggcaagtatc aattgactta ggtgcaaaag gaccaaatgg tgcaacagtt
     1621 acagcatgtg caacaggtac aaactcaatc ggagaagcat ttaaaattgt gcaacgcggt
     1681 gatgcagatg caatgattac tggtggtacg gaagctccaa tcactcatat ggcaattgca
     1741 ggtttcagtg caagtcgagc gctttctaca aatgatgaca ttgaaacagc atgtcgtcca
     1801 ttccaagaag gtagagacgg ttttgttatg ggtgaaggtg ctggtatttt agtaatcgaa
     1861 tctttagaat cagcacaagc tcgaggtgcc aatatttatg ctgagatagt tggctatggt
     1921 actacaggtg atgcttatca tattacagcg ccagctccag aaggtgaagg cggttctaga
     1981 gcaatgcaag cagctatgga tgatgctggt attgaaccta aagatgtaca atacttaaat
     2041 gcccatggta caagtactcc tgttggtgac ttaaatgaag ttaaagctat taaaaataca
     2101 tttggtgaag cagctaaaca cttaaaagtt agctcaacaa aatcaatgac tggtcactta
     2161 cttggtgcaa caggtggaat tgaagcaatc ttctcagcgc tttcaattaa agactctaaa
     2221 gtcgcaccga caatacatgc ggtaacacca gacccagaat gtgatttgga tattgttcca
     2281 aatgaagcgc aagaccttga tattacttat gcaatgagta atagcttagg attcggtgga
     2341 cataacgcag tattagtatt caagaaattt gaagcataat tataaaaatc
//
LOCUS       DAOBBH010000248         2363 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.248, whole genome shotgun sequence.
ACCESSION   DAOBBH010000248 DAOBBH010000000
VERSION     DAOBBH010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.248"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..1110
                     /locus_tag="P6L01_002483"
     CDS             <1..1110
                     /locus_tag="P6L01_002483"
                     /inference="COORDINATES: protein motif:HMM:TIGR01168.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014533320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDP3031521.1"
                     /translation="QQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKSKDF
                     TRGYEQNVFAKSELNANKNTTKDKIKNEGAVKTSDTSLKLDNKSAISNGNEINQDIKI
                     SNTPKNSSQGNNLVINNNEPTKEIKIANLEAQNSNQKKTNKVTNNYFGYYSFREAPKT
                     QIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFIGQKLKVPMTPLVEPKPKTV
                     SSNNKSNSNSSTLNYLKTLENRGWDFDGSYGWQCFDLVNVYWNHLYGHGLKGYGAKDI
                     PYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRFGGGYGHTAIVLNGDYDGKLMKFQSL
                     DQNWNNGGWRKAEVAHKVVHNYENDMIFIRPFKKA"
     gene            1138..>2363
                     /gene="fmhC"
                     /locus_tag="P6L01_002484"
     CDS             1138..>2363
                     /gene="fmhC"
                     /locus_tag="P6L01_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HDP3031522.1"
                     /translation="MKFSTLSEEEFTNYTKKHFKHYTQSIELYNYRNKINHEAHIVGV
                     KNDKNEVIAACLLTEARIFKFYKYFYSHRGPLLDYFDAKLVCYFFKELSKFIYKNRGV
                     FILVDPYLIENLRDANGRIIKNYNNSVIVKMLGKIGYLHQGYTTGYSNKSQIRWISVL
                     DLKDKDENQLLKEMEYQTRRNIKKTIEIGVKVEDLSIEETNRFYKLFQMAEEKHGFHF
                     MNEDYFKRMQEIYKDKAMLKIACINLNEYQDKLKIQLLKIENEMMTVNRALNENPNSK
                     KNKSKLNQLNMQLSSINNRISKTEELILEDGPVLDLAAALFICTDDEVYYLSSGSNPK
                     YNQYMGAYHLQWHMIKYAKSHNINRYNFYGITGVFSNEADDFGVQQFKKGFNAHVEEL
                     IGDFIKPVRPILYKFA"
BASE COUNT          990 a          262 c          391 g          720 t
ORIGIN      
        1 caacaaagta aagtacgcta ttcaattaga aaagttagta ttggaatttt gtcaatttca
       61 ataggtatgt ttttggcatt gggtatgtcg aacaaagcat atgcagatga aattgataaa
      121 tctaaagatt ttacaagagg gtatgagcaa aatgtattcg cgaaatcaga gttaaatgct
      181 aataaaaata cgacaaaaga caaaataaaa aatgaaggtg ctgttaaaac atcggacaca
      241 agtttaaagt tagacaacaa atcagcaatt tcaaacggaa atgaaattaa tcaagatata
      301 aagatttcaa atactccgaa aaactcaagc caaggtaaca atctagttat taataacaat
      361 gaacctacta aagaaattaa aattgcaaac ttggaagctc aaaattctaa tcagaagaaa
      421 acgaataaag ttactaataa ttactttggt tactacagtt ttagagaagc tccaaaaaca
      481 caaatctata ctgtaaaaaa aggagacaca cttagtgcta tagcattaaa atacaaaact
      541 acagtttcaa atattcaaaa tacaaataat atagcaaatc ctaatttaat atttattggt
      601 caaaaattaa aagtgccaat gacaccatta gtagaaccaa aaccaaaaac agtgtcttca
      661 aataataaaa gtaatagtaa tagcagtaca ttaaattatt tgaaaacatt agagaataga
      721 ggatgggatt tcgacggtag ttatggatgg caatgtttcg atttagttaa tgtatattgg
      781 aatcatcttt atggtcatgg attaaaagga tatggagcta aagatatacc atatgcaaat
      841 aattttaata gtgaagctaa aatttatcac aacacaccaa ctttcaaagc tgaacctggg
      901 gacttagtgg tttttagtgg aagatttggt ggaggatatg gtcatacagc tattgtctta
      961 aatggtgatt atgatggaaa attaatgaag ttccaaagtt tagatcaaaa ctggaataat
     1021 ggtggatggc gtaaagcaga ggttgcacat aaagttgttc ataattatga aaatgatatg
     1081 atttttatta gaccatttaa aaaagcataa tttaaatcga aggcaggaca ttgaaatatg
     1141 aaattttcaa ctttaagtga agaagaattt accaactaca ccaaaaagca cttcaaacat
     1201 tatacacagt ctatagaatt atataattat agaaataaaa taaatcatga agcacatatt
     1261 gtgggagtga agaatgataa aaatgaagtt atagctgcat gtttattaac agaggcacga
     1321 atttttaaat tctacaaata tttctactct catagaggtc ctttacttga ttatttcgat
     1381 gctaaattag tttgttactt ttttaaagaa ttatctaaat tcatttataa aaatagagga
     1441 gtatttattc ttgttgatcc atatttaata gagaatttaa gagatgcaaa tggtaggata
     1501 ataaagaatt ataataattc agtgatagta aagatgctag ggaaaattgg gtatctccat
     1561 caaggttata caacaggata ttcaaataaa agtcaaatta ggtggatttc tgtattggat
     1621 ttaaaagata aagatgagaa tcaactttta aaagaaatgg aataccaaac tagaagaaat
     1681 ataaaaaaga ctattgagat tggtgttaag gttgaagatt tatctattga agaaacaaat
     1741 cgattttata aattgtttca aatggctgaa gaaaaacatg gttttcattt catgaatgaa
     1801 gattatttta aacgaatgca agaaatatat aaagataagg caatgttaaa gatagcttgt
     1861 ataaatctta atgaatatca agataaatta aaaatacaat tattgaaaat cgaaaatgaa
     1921 atgatgactg tgaacagagc attaaatgaa aatccaaatt ctaaaaagaa taaatcaaaa
     1981 ttaaatcagt taaatatgca attatctagt attaataata gaattagtaa aaccgaagaa
     2041 ctaatattag aagatggacc tgttttggat ttagctgctg ctttatttat atgtactgat
     2101 gatgaagttt attatctatc aagtggatca aatccgaaat ataatcagta tatgggtgca
     2161 tatcatctac aatggcatat gataaaatat gcaaaatcac ataatattaa taggtataat
     2221 ttttatggaa taacaggcgt ctttagtaat gaggcggatg attttggtgt tcaacaattt
     2281 aaaaagggtt ttaatgcaca tgttgaagaa ttaattggtg atttcatcaa accagtaaga
     2341 ccaattctat ataaatttgc aaa
//
LOCUS       DAOBBH010000249         2354 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.249, whole genome shotgun sequence.
ACCESSION   DAOBBH010000249 DAOBBH010000000
VERSION     DAOBBH010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.249"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..476
                     /locus_tag="P6L01_002485"
     CDS             <1..476
                     /locus_tag="P6L01_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HDP3031523.1"
                     /translation="LIGDNIFTFFYKKVAVLFIECLIYIILGITIISIIYSDFSHYLL
                     LIILFSLVILQYILVVGTISMVSPNILISLGISIVYWIGSVILVAINKSIFGIVAPFE
                     ASNTMYRAVEKILNNESTFICPTEIINTVSFFVLLFIVNTIVLLLSRKRWLKIGM"
     gene            complement(638..1258)
                     /gene="srtA"
                     /locus_tag="P6L01_002486"
     CDS             complement(638..1258)
                     /gene="srtA"
                     /locus_tag="P6L01_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase SrtA"
                     /protein_id="HDP3031524.1"
                     /translation="MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQ
                     YDKNVKEQASKDNKQQAKPQIPKDKSKVAGYIEILDADIKEPVYPGPATPEQLNRGVS
                     FAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRD
                     VKPTDVEVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK"
     gene            1452..1943
                     /locus_tag="P6L01_002487"
     CDS             1452..1943
                     /locus_tag="P6L01_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDP3031525.1"
                     /translation="MIRCAKKEDLNAILAIYNDAIINTTAVYTYKPQTIDERIAWFET
                     KQRNHEPIFVFEENGSVLGFATFGSFRPWPAYQYTIEHSIYVDASARGKGIASQLLQR
                     LIVEAKAKGYRTLVAGIDASNEASIKLHQKFNFKHAGTLTNVGYKFDYWLDLAFYELD
                     LKD"
     gene            complement(2087..2257)
                     /locus_tag="P6L01_002488"
     CDS             complement(2087..2257)
                     /locus_tag="P6L01_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031526.1"
                     /translation="MLRLSIIFIAFIINTTITYGYTTEGTWVNLLFKSLSLSMIIVFM
                     FYYIRFVIEKKR"
BASE COUNT          731 a          399 c          348 g          876 t
ORIGIN      
        1 agctgattgg cgataatata tttaccttct tctataaaaa agtagcagtt ctattcattg
       61 aatgtttaat ttatattatt ttaggcatca caattatttc aataatatat tcggatttct
      121 cacattactt actattaata atcttatttt cactcgttat cttacaatat atcttagttg
      181 ttggaacgat tagtatggtt tcacctaaca ttttaatcag cttaggtatc agcattgtat
      241 attggattgg ttcagtcatc ttagttgcca ttaataaaag tatatttggt atcgttgctc
      301 cgtttgaggc gagcaacacg atgtatcgtg ccgtagaaaa aatattgaat aacgagtcaa
      361 catttatatg tcctactgaa attataaata cagtatcatt cttcgttctt ctatttatag
      421 ttaatactat tgtcttatta ctaagtagaa agagatggtt gaaaatcggt atgtagtaca
      481 taaaaaagag cggcatccat tgtcgctcct ttttattatg tatttttaca atctccacca
      541 atgacacgtc gtcattggtg cctcacacca aacaaaaaaa cagtctcgca atcaagactg
      601 ttttccactc aatatattca tccattagcg taatggatta tttgacttct gtagctacaa
      661 agattttacg tttttcccaa acgcctgtct tttcattgta atcatcacaa gtaattaatg
      721 ttaattgttt atctttacct ttttgttcat ctagaacttc tacatctgtt ggcttaacat
      781 ctcttatact tgtcatttta tacttacgtg tttcattacc aactttaaag tacaccatac
      841 tacctttttt ggctgcttta agatttgtaa attgatagtt cggacggtca atgaaagtgt
      901 gtcctgcaat tgaaatattt tgatcatcta gtgattcatt ttcttctgca aagcttacac
      961 ctctatttaa ttgttcaggt gttgctggtc ctggatatac tggttcttta atatcagcat
     1021 ctagaatttc aatatagcct gccacttttg atttatcttt cggaatttga ggtttagctt
     1081 gctgcttatt gtctttactc gcctgttctt ttacattttt atcatattgt tcaatctttt
     1141 catctttatc tttatcgtga agataattat cgatatgtgg tttagcaaac aaatatgctg
     1201 ccactaggat aagtactaca ccagcgattg tcattaatcg atttgtccat tttttcatac
     1261 gttaaggctc cttttataca tttcacaatg cctattaaca tttcatatag tataccatta
     1321 attataaaat gactcatagc aattcatttt atattataaa atttgcacat atattgtcac
     1381 gttagatttc attacacata tttgcattca aataacgaaa cgtttttaat aattactaag
     1441 ggggaattat tatgattcga tgtgctaaaa aagaagattt aaacgctata ttagcgatat
     1501 acaacgatgc cattattaat acaacagctg tttatactta taaaccgcaa accatagacg
     1561 aacgtatcgc atggtttgaa acgaaacaac gtaatcatga gcctatcttt gtatttgagg
     1621 aaaatggaag tgtattaggg tttgcgacgt tcggttcgtt tagaccttgg ccagcatatc
     1681 aatatactat cgagcattct atttatgtcg atgcttcagc tagaggaaaa ggtatcgcta
     1741 gtcaattatt acagcgttta attgtagagg caaaagctaa aggttatcgt acacttgttg
     1801 ctggtattga tgcttctaat gaagcgagta ttaaattaca tcaaaaattc aatttcaagc
     1861 atgccggcac actgaccaat gtaggctata aatttgatta ctggttagat ttagcttttt
     1921 atgaattaga tttaaaagac tagtagtgtt taaatcacat aatataaaca agacaaccat
     1981 gttaattcac ttaacataac atgctaacat ataaaactat aaacctctta gggacaattt
     2041 acatcgtccc ttttttcatt tcaaatacta tccccatagc tttgatttaa cgctttttct
     2101 caataacaaa acgaatatag tagaacatga aaacgataat catgctgagc gataaagatt
     2161 taaataatag attgacccac gttccctcag tcgtatatcc atatgtaatc gttgtgttaa
     2221 tgatgaatgc tataaagatg attgatagtc ttagcatatc atcactcctt ttaagttatt
     2281 ttagatatac gggggcgctt ttgcaatcac tattttgatt agcatgtatt tcccataaat
     2341 ttttcaactt tatg
//
LOCUS       DAOBBH010000250         2325 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.250, whole genome shotgun sequence.
ACCESSION   DAOBBH010000250 DAOBBH010000000
VERSION     DAOBBH010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.250"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..68
                     /locus_tag="P6L01_002489"
     CDS             <1..68
                     /locus_tag="P6L01_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDP3031527.1"
                     /translation="SSNNVDILKRISNLEKAVFGK"
     gene            131..510
                     /gene="rnpB"
                     /locus_tag="P6L01_002490"
     ncRNA           131..510
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="P6L01_002490"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            719..1864
                     /locus_tag="P6L01_002491"
     CDS             719..1864
                     /locus_tag="P6L01_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDP3031528.1"
                     /translation="MFQLLAVCPMGLEAVVAREIQELGYETNVENGRIFFEGDASAIV
                     KANLWLRTADRIKIVVGRFNATTFDELFEQTKALPWESIIDKEGNFPVQGRSVKSTLH
                     SVPDCQAITKKAIVERLRRAYNEKGWLNESGAKYPVEVAILKDNVLLTIDTSGSGLNR
                     RGYRLAQGEAPIKETLAASLIRLANWKGDTPLIDPFCGSGTIAIEACLIAQNIAPGFN
                     REFVSEQWNIMPANIYDDYRDEADKMADYDKEIEVYASDIDPEMVEIAKRNAEEVGLS
                     DIIKFSVKDVNTLTIDTEEPVALIGNPPYGERIGDREEVEEMYRYIGKLMKQHPFLST
                     YILTSNKEFEYLVNRKATKRRKLFNGYIECTYYQYWGKKTERKTIEN"
     gene            1959..2279
                     /locus_tag="P6L01_002492"
     CDS             1959..2279
                     /locus_tag="P6L01_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HDP3031529.1"
                     /translation="MGLGLSIALVVIGIAVVCLMIFSSQKTTYFGYMNSKTNAEKVVS
                     EKDGLVKHNIKVEPSNDFKPKKGDFVKLVSKDDGKTFYKQEIVKHDDVPHGLMMKIHD
                     MHMN"
BASE COUNT          842 a          312 c          470 g          701 t
ORIGIN      
        1 catcttcaaa taatgtagat attttaaaac gtatttcaaa cttagaaaaa gctgtatttg
       61 gtaaataatt actaaataca aaagtttaac tgtcttacta ataatgacta tgttataatt
      121 ttaaaagtga tattttgggt aatcgctata ttatatagag gaaagtccat gctcacacag
      181 tctgagatga ttgtagtgtt cgtgcttgat gaaacaataa atcaaggcat taatttgacg
      241 gcaatgaaat atcctaagtc tttcgatatg gatagagtaa tttgaaagtg ccacagtgac
      301 gtagctttta tagaaatata aaaggtggaa cgcggtaaac ccctcgagtg agcaatccaa
      361 atttggtagg agcacttgtt taacggaatt caacgtataa acgagacaca cttcgcgaaa
      421 tgaagtggtg tagacagatg gttatcacct gagtaccagt gtgactagtg cacgtgatga
      481 gtacgatgga acagaacatg gcttatagaa atatcactac tagtttagct ctcctagatg
      541 atggagagct tttttcatga aaagaacact taaaattaac gccttgtctt gatataatga
      601 cactgccttg ttttaaaata gtaagcggat gcgttaatgt atcagcgatt aaatttgttg
      661 gaaatgtata aaaaacacaa gctaagaata aaatacctgt ataaaaggag aatcatatat
      721 gtttcaatta cttgcagttt gtccgatggg attagaagct gttgttgcta gggaaattca
      781 agaattaggc tatgaaacaa atgttgaaaa tggtcgtata ttttttgaag gagacgcaag
      841 tgcaattgta aaggcaaatt tatggttgcg cacagcagac cgaatcaaaa ttgttgttgg
      901 acgttttaac gcaacaacgt ttgacgaatt attcgaacaa accaaagcgc tcccttggga
      961 atctataatt gataaagaag gtaacttccc agttcaaggt agaagcgtta aatcaacact
     1021 acatagtgtg ccagattgtc aggcgataac taagaaagct attgttgaac gattacgtcg
     1081 cgcatataac gaaaaaggtt ggttaaatga atcaggtgcc aaataccctg ttgaagttgc
     1141 tattttaaaa gataatgtat tattgactat cgacacatca ggttctggtt tgaacagacg
     1201 tggttataga ttagcacaag gtgaagcacc aattaaagaa acgttggcag caagtttaat
     1261 ccgtcttgca aactggaaag gtgatacacc tttaattgat ccattttgcg gttcgggtac
     1321 aatagctata gaagcttgtt taattgctca aaatatcgca cctggtttta atcgcgagtt
     1381 cgtatcagag caatggaaca tcatgccagc aaatatttat gatgattacc gtgatgaagc
     1441 ggataagatg gctgattatg ataaagaaat cgaagtatat gcttctgata tcgatccaga
     1501 aatggtagag attgctaagc gtaacgctga agaagttggg ttgtctgata ttattaaatt
     1561 tagtgtaaaa gatgtcaata cattaacaat tgatacagaa gaaccggtgg cgttaattgg
     1621 aaatcctcca tatggtgaac gtattggtga tcgtgaagaa gttgaagaaa tgtaccgtta
     1681 tattggtaaa ctaatgaaac aacatccatt tttatctaca tacattttaa caagtaataa
     1741 agaatttgaa tatttagtaa atcgtaaagc aacgaagcga cgtaagttat ttaatggata
     1801 tatagaatgt acttattatc aatactgggg taaaaagaca gaacgtaaaa ctattgaaaa
     1861 ctaaagtgtt tctaatgcgt gactaaaatt agtaataatt aagttctcat gataataggt
     1921 atttttgaaa aatggaggag tctataaatg ggtaaaaaat gggtctaggt ttatctatag
     1981 cattggttgt tattggtatt gccgttgtat gtttaatgat tttttctagt caaaaaacga
     2041 cttattttgg ttatatgaat agtaaaacaa atgcagaaaa agttgtcagt gaaaaagatg
     2101 gattagtcaa acataatatc aaagtagaac catcaaatga ttttaaaccg aaaaaaggag
     2161 actttgtaaa attagtttct aaagatgatg ggaagacatt ttataaacaa gagattgtta
     2221 aacatgatga cgtcccacac ggtttaatga tgaaaattca cgacatgcat atgaattaat
     2281 aaaaaagcat ctataacgta attttgaaga agtagagtta tcttc
//
LOCUS       DAOBBH010000251         2320 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.251, whole genome shotgun sequence.
ACCESSION   DAOBBH010000251 DAOBBH010000000
VERSION     DAOBBH010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.251"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..120
                     /locus_tag="P6L01_002493"
     CDS             <1..120
                     /locus_tag="P6L01_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HDP3031530.1"
                     /translation="RITMKNGGYYTFELNKKLQPHRMGDTIDSRNIEKIEVNL"
     gene            142..1629
                     /locus_tag="P6L01_002494"
     CDS             142..1629
                     /locus_tag="P6L01_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKLRK protein"
                     /protein_id="HDP3031531.1"
                     /translation="MRENFKLRKMKVGLVSVAITMLYIMTNGQAEASETNQKVSTNQE
                     SKAVSQTEQNSKETKAAESSKNFVNLDPIKPGAQKVTGTTLPNHIILLNIDGKSADSV
                     EGGYGDFITANDKGEFEYPLNNRKIVHNQEIEVSSSSPDLGDDEEDEEVEESSTEKAG
                     AEEESTDAKATYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKGHTSVKGK
                     VALSINNKFINFEERAKGGISKEDTKASSDGVWMPINEKGYFDFDFKKNPFDNLELKK
                     NDEISLTFAPDDEDEALKSLIFKTKVTSLEDIDKAETKYDHTKVEKVKVLKDVKEDIH
                     VDEIYGSLYHTEKGKGILDKQGTKEITGKTKFANAVVKVYSDLGDAQLFPDIQVDENG
                     KFSFDAEKAGFRLQNGETLNFAVVKPITGDLLHQGFVSKYIDVYESPEEKKEREFEEK
                     LENTPAYHKLHGDKIVGYDVQGNPSTWFYPLGEKKVERTTPKLEK"
     gene            complement(1738..2046)
                     /gene="spn"
                     /locus_tag="P6L01_002495"
     CDS             complement(1738..2046)
                     /gene="spn"
                     /locus_tag="P6L01_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myeloperoxidase inhibitor SPIN"
                     /protein_id="HDP3031532.1"
                     /translation="MKFKKVLVATAMVGVLATGVVGYGNQADAKVYSQNGLVLHDDAN
                     FLEHELSYIDVLLDKNADQATKDNLRSYFADKGLHSIKDIINKAKQDGFDVSKYEHVK
                     "
BASE COUNT          911 a          305 c          399 g          705 t
ORIGIN      
        1 agaattacta tgaaaaacgg tggctattat acatttgaat taaataaaaa attacagcct
       61 catcgcatgg gtgatacgat tgatagtaga aatatagaga aaattgaagt aaatttataa
      121 taatattcga gggagtatat catgagagaa aattttaaat tacgtaaaat gaaagtcggt
      181 ttagtatcag ttgcaattac tatgttatat atcatgacaa acggacaagc tgaagcatct
      241 gaaactaatc agaaggtaag tacaaatcaa gaatcaaaag ctgtttcgca gactgaacaa
      301 aattccaaag aaacaaaagc ggcagagtct tctaaaaact ttgtaaattt agatcctatc
      361 aaaccaggag ctcaaaaagt aacaggtact acattaccga atcatattat tttacttaat
      421 atagatggta aaagtgctga ttcagttgaa ggaggatatg gtgattttat aactgcaaat
      481 gacaaaggag agtttgagta tcctttaaat aatcgtaaaa ttgtccataa ccaagaaatt
      541 gaggtatcat catcaagccc tgatttaggt gacgatgaag aggatgaaga agtagaagaa
      601 tcttcaactg aaaaagctgg tgctgaggaa gaaagtacag atgctaaagc tacatataca
      661 acaccacgat atgaaaaagc gtatgaaata ccgaaagaac aactaaaaga aaaagatgga
      721 caccaccaag ttttcatcga accaattact gaaggttcag gtattattaa aggtcatacg
      781 tctgtaaaag gtaaagttgc tctatctatt aataataaat ttattaattt tgaagaaaga
      841 gctaaaggtg gaattagtaa agaagatact aaagctagtt ccgatggtgt ttggatgcct
      901 attaatgaaa aaggatattt tgattttgat ttcaaaaaaa atccttttga taacttagag
      961 ttaaagaaaa atgatgaaat ctcattaaca tttgcacctg atgatgaaga tgaagcattg
     1021 aagtcattaa ttttcaaaac taaagtaacg agcttagaag atatcgataa agcagaaaca
     1081 aaatatgacc atactaaagt ggaaaaagta aaagtattga aagatgttaa agaagatata
     1141 catgtagatg aaatttacgg aagcttatat catacagaaa aaggtaaagg tattcttgat
     1201 aaacaaggaa ctaaagagat tacagggaag actaaattcg cgaatgcagt agtgaaagta
     1261 tattctgact taggtgatgc gcaattgttc cctgatattc aagtagatga aaatggtaaa
     1321 tttagctttg atgctgaaaa agctggtttc agattgcaaa atggagaaac attgaatttt
     1381 gcagtagtta aacctattac tggtgaccta ttacatcaag gatttgtctc taaatatatt
     1441 gatgtatatg aatctccgga agaaaagaaa gaacgtgaat ttgaagaaaa acttgaaaac
     1501 acgcctgcat atcataaatt acatggtgat aaaattgtag gatatgatgt tcaaggtaat
     1561 ccatcaactt ggttctatcc attaggtgaa aagaaagttg aacgtacaac acctaaatta
     1621 gaaaaataat taataaaata aaacagctta atgatgtaat gaaattagtg agttaatcac
     1681 tgacttcaac gtcattgagc tgttttttat ttgcttcgtt acaaagcatt attgaattta
     1741 ttttacgtgt tcatattttg aaacatcaaa gccgtcttgc ttagctttgt tgataatgtc
     1801 tttgattgaa tgtagtcctt tatcggcgaa gtatgatctt aagttgtctt ttgtagcttg
     1861 gtcagcattc ttatctaata acacatcgat atagcttaat tcatgttcta agaagtttgc
     1921 gtcatcatgt agtacgagtc cattttgaga ataaactttc gcatctgctt gattaccata
     1981 tccaacaacg ccagttgcta atacacctac cattgccgta gctactaaaa cctttttaaa
     2041 tttcatatct atcactcctc taaaaattgt aactctatca taacactgaa tattaagaaa
     2101 attacgttta ttaagtcgat ttaataattt ttaataaata gttaaactga caaatattgc
     2161 ttaaatgcaa ttaatcttta aaacggtgtt tatggatttt tcctactaaa accttgattt
     2221 caaaaagggt ttaaatcaaa tgaaacaata ataaaaaata atgcaacata ataataagta
     2281 caaatttaat taagaaatta aattgattgt atatgtatat
//
LOCUS       DAOBBH010000252         2266 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.252, whole genome shotgun sequence.
ACCESSION   DAOBBH010000252 DAOBBH010000000
VERSION     DAOBBH010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.252"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            118..>2266
                     /locus_tag="P6L01_002496"
     CDS             118..>2266
                     /locus_tag="P6L01_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDP3031533.1"
                     /translation="MAKSCLHILTNNEYATTRCQDGIVLFWPIDGEIELQKFRKSKII
                     EDDIYIINHLDVFSIKNNKKTIMLYLSSDWFAELGFTFFNYHYTAKLIKSSYNLKCLL
                     LKLTYRYLDNQPLNDADIRKLQDIIKIIAKEASMDKKIAQNQYRYAYYGDLRDELEYI
                     YQNVNQRLTLKSVADKLFVSKSNLSSQFHLLMGMGFKKYIDTLKIGKSIEILLTTDST
                     ISNISEHLGFSSSSTYSKMFKSYMDITPNEYRNLSKYNKCLMLKPEPLVGKMVQEVKE
                     IILNYIEHYKNHLTDVIHIDEDKFETPKLFQTVIQINTYTEMKLVFLEGIFKTLLNKN
                     SQVVFFIMPSILKSKNTMSEEEKFTIIKTIIESDLKIAFNINDIETTYFVEEAFMSVF
                     RQISPNELSNHNNYEVHFVFDLSLMEIRTIYRMILKLHNIMLNVKLGLNITCLFEKPS
                     VFKSLVSQIKRLKFDSLIIDNANLSSPYLMGESDELLLKNILHFKNLKQVINELDIEQ
                     EKLIFLNVENHKLLNNKERDLSNSAPLIYKTLSALYHNFDGFGLNIFDNHHTFNAMHL
                     YDKNGFKTTLGLILEKFIEYVSKPKYENSYYSIFDIENYYCLVIYDWRVIESETIMSN
                     FEDSQVYINFKNNVLNDKYLIVIETLDENSGNINHLISKELRDKYEWNPSLLSKIDNY
                     LKPAIEIKEHNFSDNSLNINVTFNALYIIKIGKK"
BASE COUNT          894 a          264 c          339 g          769 t
ORIGIN      
        1 agttttattt atttttgatt tggtattcgt attgtgttaa atttacgttt aattaaataa
       61 gttaaatatt atagggaatg ataaaagtgg tacttaaata tagaaagagg taaagttatg
      121 gcaaaatcat gcttgcatat acttactaat aatgaatatg cgacaacgcg ttgccaagat
      181 ggcatagtct tattttggcc aattgacggg gaaatcgaac tacaaaaatt tcgtaaaagt
      241 aaaataattg aagatgatat atatattatt aatcatctgg atgtatttag tattaagaat
      301 aataaaaaaa cgatcatgtt gtatttgagt agcgattggt ttgcggaatt aggctttact
      361 ttctttaatt accactatac agcaaagttg attaaatcat cctataattt gaaatgtcta
      421 ctattaaaat tgacttatcg atatcttgat aatcaacctc tcaatgacgc tgatattaga
      481 aaattacagg atattattaa aatcattgca aaagaagcaa gtatggataa aaagattgcg
      541 caaaatcaat atcgatatgc ctattatggt gatttacgtg acgagctcga atatatttat
      601 caaaatgtaa atcaacgatt gacattaaaa agtgtcgctg ataaattatt tgtctcaaag
      661 tcaaatttgt catcacaatt ccacttactt atgggcatgg gttttaaaaa atatattgat
      721 actttgaaaa ttggtaaatc gattgaaatt ctacttacta ctgatagtac tattagcaac
      781 ataagtgagc atttaggttt tagtagtagc tccacttact ctaaaatgtt taaaagttat
      841 atggatataa caccgaatga atatcgtaat ttatcaaaat ataataaatg tttaatgcta
      901 aagccagaac cactagtagg caaaatggtg caagaagtaa aagaaatcat attgaattat
      961 attgaacatt ataaaaacca cctaactgat gttatacata ttgatgaaga caaatttgaa
     1021 acacctaaat tgtttcaaac ggttattcaa ataaatactt atacagaaat gaaattagtt
     1081 ttcttagaag gaatctttaa aaccttattg aataagaaca gtcaagttgt ctttttcatc
     1141 atgccatcga ttctaaaaag taaaaatacc atgtccgaag aagaaaaatt cacaatcatt
     1201 aaaacaataa ttgaaagtga tctaaagata gcatttaata taaatgatat tgaaacaact
     1261 tattttgttg aagaagcttt tatgagtgtt ttcagacaaa tatctccaaa cgaattaagt
     1321 aatcataata attacgaagt gcattttgtt tttgatttat cattgatgga aattagaaca
     1381 atttatcgaa tgatattaaa attacataac atcatgttga atgtgaaatt aggattgaac
     1441 attacctgtt tatttgaaaa accttcagtt tttaaatcac tagtatcaca aataaagcga
     1501 cttaaattcg attcgttaat aatagataat gcaaatttaa gtagccctta tttgatgggg
     1561 gaaagtgatg agttactatt gaaaaatatt ttgcatttta aaaatttaaa acaagtaatt
     1621 aatgaattgg atattgaaca agaaaagctt atttttctaa atgttgaaaa tcataaactg
     1681 cttaataata aagaacgaga tttaagtaat agtgctccat taatttataa gacattaagt
     1741 gcgctgtatc acaactttga tggctttgga ttaaacattt ttgataatca tcatacattt
     1801 aatgcgatgc atctatatga taaaaatgga tttaaaacaa cactaggtct tatattggaa
     1861 aaatttatcg aatatgtctc gaaaccaaaa tacgaaaaca gttattattc tatttttgat
     1921 atagagaatt attattgcct ggttatttat gattggcgag tgattgagag cgagacaatt
     1981 atgagcaatt ttgaggatag tcaagtttat ataaatttca aaaataatgt tttaaacgat
     2041 aaatatctaa ttgtaataga aacattggac gaaaatagtg gcaacattaa tcatttgatt
     2101 tctaaagaat taagagataa atatgaatgg aaccctagtt tactatctaa aattgacaac
     2161 taccttaaac cagcaatcga gattaaagag cataatttta gtgataattc cttgaatatt
     2221 aacgttactt ttaatgcgtt atacataatt aaaataggaa aaaaat
//
LOCUS       DAOBBH010000253         2238 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.253, whole genome shotgun sequence.
ACCESSION   DAOBBH010000253 DAOBBH010000000
VERSION     DAOBBH010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.253"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            187..543
                     /gene="sarT"
                     /locus_tag="P6L01_002497"
     CDS             187..543
                     /gene="sarT"
                     /locus_tag="P6L01_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarT"
                     /protein_id="HDP3031534.1"
                     /translation="MNDLKSKSNIKLMKRVLTTYELRKYLKKYFCLTLDNYLVLAYLD
                     VFKNDEGKYFMRDIISYIGIDQSRIVKSVKELSKKGYLNKCRDPHDSRNVIIVVSVKQ
                     HNYIKNLLSEININET"
     gene            848..>2238
                     /gene="sasG"
                     /locus_tag="P6L01_002498"
     CDS             848..>2238
                     /gene="sasG"
                     /locus_tag="P6L01_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored surface protein SasG"
                     /protein_id="HDP3031535.1"
                     /translation="MRDKKGPVNKRVDFLSNKLNKYSIRKFTVGTASILIGSLMYLGT
                     QQEAEAAENNIENPTTLKDNVQSKEVKIEEVTNKDTAPQGVEAKSEVTSNKDTIEHEA
                     SVKAEDISKKEDTPKEVANVAEVQPKSSVTHNAEAPKVRKARSVDEGSFDITRDSKNV
                     VESTPITIQGKEHFEGYGSVDIQKNPTDLGVSEVTRFNVGNESNGLIGALQLKNKIDF
                     SKDFNFKVRVANNHQSNTTGADGWGFLFSKGNAEEYLTNGGILGDKGLVNSGGFKIDT
                     GYIYTSSMDKTEKQAGQGYRGYGAFVKNDSSGNSQMVGENIDKSKTNFLNYADNSTNT
                     SDGKFHGQRLNDVILTYVASTGKMRAEYAGKTWETSITDLGLSKNQAYNFLITSSQRW
                     GLNQGINANGWMRTDLKGSEFTFTPEAPKTITELEKKVEEIPFKKERKFNPDLAPGTE
                     KVTREGQKGEKTIT"
BASE COUNT          916 a          259 c          411 g          652 t
ORIGIN      
        1 acggaataac aaatgacaaa ttcatttatt atccaaaaac aaaaacaatt gaacaaaatc
       61 aaaatatagt gttatgattc atttgtgttt atagctagca ccacaaacta aatatatttg
      121 gagttattac tagttaaaga caaatattat ttgacgtgac aattaaaagt aagggatgaa
      181 gacttgatga atgatttgaa aagcaagagc aatattaaat taatgaaaag agtattaact
      241 acatatgagc tgcgtaaata tttaaaaaaa tatttttgtc ttacattaga taattattta
      301 gtattagcat atttagatgt atttaaaaat gatgaaggta aatattttat gagagacatt
      361 ataagttata tcggtataga ccaatctaga attgttaaaa gcgtaaaaga attatcaaaa
      421 aagggttact tgaataaatg tagagaccca catgatagta gaaatgtaat cattgttgtt
      481 tctgtaaaac aacacaatta tattaaaaat cttttgagtg aaataaatat taatgaaaca
      541 taaagtatgt ttcgagattt taatatcttg gtgcagttaa atgtattttt aaatgcgtta
      601 aggtattttt tattttagaa attcaatgct attgagttga tgggtttttc caacagataa
      661 gaatgaatta ttcattgaaa actaaaaata atatttttag ttgtagattg ttttaagtgt
      721 gtcaatatat aaaataattt tataaagata agtgttatat tccattgtgc aactcctatt
      781 gtatttgtaa aattacatgt aaattcactg taagtaaagt ggaaaatatg gaaaaaggag
      841 tatgcaaatg agagataaga aaggaccggt aaataaaaga gtagattttc tatcaaataa
      901 attgaataaa tattcaataa gaaaatttac agttggaaca gcatctattt taattggctc
      961 actaatgtat ttgggaactc aacaagaagc agaagcagct gaaaacaata ttgagaatcc
     1021 aactacatta aaagataatg tccaatcaaa agaagtgaag attgaagaag taacaaacaa
     1081 agacactgca ccacaaggtg tagaagctaa atctgaagta acttcaaaca aagacacaat
     1141 cgaacatgaa gcatcagtaa aagctgaaga tatatcaaaa aaggaggata caccaaaaga
     1201 agtagctaat gttgctgaag ttcagccgaa atcgtcagtc actcataacg cagaggcacc
     1261 taaggttaga aaagctcgtt ctgttgatga aggctctttt gatattacaa gagattctaa
     1321 aaatgtagtt gaatctaccc caattacaat tcaaggtaaa gaacattttg aaggttacgg
     1381 aagtgttgat atacaaaaaa acccaacaga tttaggggta tcagaggtaa ccaggtttaa
     1441 tgttggtaat gaaagtaatg gtttgatagg agctttacaa ttaaaaaata aaatagattt
     1501 tagtaaggat ttcaatttta aagttagagt ggcaaataac catcaatcaa ataccacagg
     1561 tgctgatggt tgggggttct tatttagtaa aggaaatgca gaagaatatt taactaatgg
     1621 tggaatcctt ggggataaag gtctggtaaa ttcaggcgga tttaaaattg atactggata
     1681 catttataca agttccatgg acaaaactga aaagcaagct ggacaaggtt atagaggata
     1741 cggagctttt gtgaaaaatg acagttctgg taattcacaa atggttggag aaaatattga
     1801 taaatcaaaa actaattttt taaactatgc ggacaattca actaatacat cagatggaaa
     1861 gtttcatggg caacgtttaa atgatgtcat cttaacttat gttgcttcaa ctggtaaaat
     1921 gagagcagaa tatgctggta aaacttggga gacttcaata acagatttag gtttatctaa
     1981 aaatcaggca tataatttct taattacatc tagtcaaaga tggggcctta atcaagggat
     2041 aaatgcaaat ggctggatga gaactgactt gaaaggttca gagtttactt ttacaccaga
     2101 agcgccaaaa acaataacag aattagaaaa aaaagttgaa gagattccat tcaagaaaga
     2161 acgtaaattt aatccggatt tagcaccagg gacagaaaaa gtaacaagag aaggacaaaa
     2221 aggtgagaag acaataac
//
LOCUS       DAOBBH010000254         2217 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.254, whole genome shotgun sequence.
ACCESSION   DAOBBH010000254 DAOBBH010000000
VERSION     DAOBBH010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.254"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            136..918
                     /gene="seo"
                     /locus_tag="P6L01_002499"
     CDS             136..918
                     /gene="seo"
                     /locus_tag="P6L01_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type O"
                     /protein_id="HDP3031536.1"
                     /translation="MKNSKVMLNVLLLILNLIAICSVNNAYANEEDPKIESLCKKSSV
                     DPIALHNINDDYINNRFTTVKSIVSTTEKFLDFDLLFKSINWLDGISAEFKDLKVEFS
                     SSAISKEFLGKTVDIYGVYYKAHCHGEHQVDTACTYGGVTTHENNKLSEPKNIGVAVY
                     KDNVNVNTFIVTTDKKKVTAQELDIKVRTKLNNAYKLYDRMTSDVQKGYIKFHSHSEH
                     KESFYYDLFYIKGNLPDQYLQIYNDNKTIDSSDYHIDVYLFT"
     gene            1199..1918
                     /gene="sem"
                     /locus_tag="P6L01_002500"
     CDS             1199..1918
                     /gene="sem"
                     /locus_tag="P6L01_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_058008129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type M"
                     /protein_id="HDP3031537.1"
                     /translation="MKRILIIVVLLFCYSQNHIATADVGVLNLRNYYGSYPIEDHQSI
                     NPENNHLSHQLVFSMDNSTVTAEFKNVDDVKKFKNHAVDVYGLSYSGYCLKNKYIYGG
                     VTLAGDYLEKSRRIPINLWVNGEHQTISTDKVSTNKKLVTAQEIDTKLRRYLQEEYNI
                     YGFNDTNKGRNYGNKSKFSSGFNAGKILFHLNDGSSFSYDLFDTGTGQAESFLKIYND
                     NKTVETEKFHLDVEISYKDES"
     gene            1953..>2217
                     /locus_tag="P6L01_002501"
     CDS             1953..>2217
                     /locus_tag="P6L01_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031927819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HDP3031538.1"
                     /translation="MKKFKYSFILVFILLFNIKDLTYAQGDIGVGNLRNFYTKHDYID
                     LKGVTDKNLPIANQLEFSTGTNDLISESNNWDEISKFKGKKLDI"
BASE COUNT          898 a          226 c          347 g          746 t
ORIGIN      
        1 taaaatttgt ttgttcaata gtaagtagga ttgttaagtt taaaatcgta atctaataat
       61 attagtgaat atttaaagat taaaaacaga atttttcagt ttattaagaa aaaaattttg
      121 tgaaggaggg atattattaa aaatagtaaa gtaatgttaa atgtattatt attaatttta
      181 aatttaattg caatatgtag tgtaaacaat gcatatgcaa atgaagaaga tcctaaaata
      241 gagagtttgt gtaagaagtc aagtgtagac cctattgctt tacataatat taatgatgat
      301 tatataaata atcgatttac gacagtaaaa tcaattgtat caactacaga aaaattctta
      361 gacttcgatt tattatttaa aagtattaat tggttagatg gaatatctgc tgaatttaaa
      421 gatttaaaag tggaatttag ctcatcagcg atttctaaag aatttctagg aaagactgtt
      481 gatatttatg gtgtttacta taaagcacat tgtcatggtg agcatcaagt ggatactgcc
      541 tgtacatatg gtggggtaac aactcatgaa aataataaat taagcgagcc taaaaatata
      601 ggagtagctg tgtataagga taatgtaaat gttaatacat ttatcgttac tacagataaa
      661 aagaaagtta ctgcacaaga acttgatatt aaagtaagaa caaaattaaa taatgcatat
      721 aaattgtatg acagaatgac tagtgatgta caaaaaggtt atattaaatt tcattctcat
      781 tcggagcata aagaatcatt ttattatgat ttattttata ttaaaggaaa tttaccagat
      841 caatatttgc aaatttataa tgataataaa acaatagatt catcagacta tcatattgat
      901 gtttatttat ttacataaca atctgaaagt tgatggatat caacttacaa tcctataaag
      961 aactatagat attaacatat ttttacaaag tagcttagta aatgatgaag aacgaatagt
     1021 gattcaaatc taactttatt actataaaaa gctttctatt ttagtataga taacaaaacc
     1081 agattaacga gtaataatat ataagtggga tagaatcatg atgatttaag aagaattggt
     1141 tatttttaca ataaaagata ataaagatag agaagtttaa atataggaga aataaaatat
     1201 gaaaagaata cttatcattg ttgttttatt gttttgctat tcgcaaaatc atatcgcaac
     1261 cgctgatgtc ggagttttga atcttaggaa ctattatggt agctatccaa ttgaagacca
     1321 ccaaagtatt aatcctgaaa ataatcatct ttcgcatcaa ttagtttttt ctatggataa
     1381 ttcgacagta acagctgaat ttaagaacgt tgatgatgta aagaaattca aaaatcatgc
     1441 tgtagatgta tatggtctaa gttatagtgg atattgtttg aaaaacaaat atatatacgg
     1501 tggagttaca ttagcaggtg attatttaga gaaatctaga cgtattccta ttaatctttg
     1561 ggttaatgga gaacatcaaa ctatatctac tgacaaagta tcaactaata aaaagttagt
     1621 aacagctcaa gaaattgata ctaaattaag aagatatcta caagaagaat ataatattta
     1681 tggctttaat gatacaaata aaggaagaaa ttatggtaat aagtcaaaat ttagttctgg
     1741 atttaatgca ggaaaaatat tatttcattt gaatgatggt tcatcatttt cttatgactt
     1801 atttgatact ggaacaggac aagctgaaag tttcttaaaa atatataatg acaacaaaac
     1861 tgtcgaaact gaaaaattcc atttagatgt agaaatatct tataaggacg aaagttgaag
     1921 tatttcaatc ataacttagt aaaggaaatg ccatgaaaaa atttaaatat agttttatat
     1981 tagtttttat attacttttt aacattaaag atcttacgta tgctcaaggt gatattggtg
     2041 taggtaactt aagaaatttc tatacaaaac atgattatat agatttaaaa ggcgtcacag
     2101 ataaaaacct acctattgca aatcaactcg aattttcaac aggtaccaat gatttgatct
     2161 cagaatctaa taattgggac gaaataagta aatttaaagg aaagaaactg gatattt
//
LOCUS       DAOBBH010000255         2178 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.255, whole genome shotgun sequence.
ACCESSION   DAOBBH010000255 DAOBBH010000000
VERSION     DAOBBH010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.255"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..137)
                     /locus_tag="P6L01_002502"
     CDS             complement(<1..137)
                     /locus_tag="P6L01_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031539.1"
                     /translation="MSFMDKAKDAVEKFKNSDNEQVKNVKDKINEYTGSNNEEKKENE
                     D"
     gene            complement(177..359)
                     /locus_tag="P6L01_002503"
     CDS             complement(177..359)
                     /locus_tag="P6L01_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDP3031540.1"
                     /translation="MADESKFDQFKGNVKETVGNVTDNKELEKEGQQDKATGKAKEVV
                     ENAKNKITDAIDKLKK"
     gene            complement(459..881)
                     /locus_tag="P6L01_002504"
     CDS             complement(459..881)
                     /locus_tag="P6L01_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDP3031541.1"
                     /translation="MKISTKGRYGLTLMISLAKKEGQGCISLKSIAEENNLSDLYLEQ
                     LVGPLRNAGLIRSVRGAKGGYQLRVPAEEISAGDIIRLLEGPITFVESIESEPPAQKQ
                     LWIRMRDAVRDVLDNTTLKYLAEYVDTSEDLDGYMFYI"
     gene            966..>2178
                     /locus_tag="P6L01_002505"
     CDS             966..>2178
                     /locus_tag="P6L01_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDP3031542.1"
                     /translation="MSTEPLASRMRPKNIDEIISQQHLVGPKGIIRRMVDTKKLTSMI
                     FYGPPGIGKTSIAKAISGSTQYKFRQLNAVTNTKKDMQLVVEEAKMSGQVILLLDEIH
                     RLDKAKQDFLLPHLENGKIVLIGATTSNPYHAINPAIRSRAQIFELYPLNDEDVRQAL
                     TRAIEDDENGLKTYQPKIDEDAMTYFSTQSQGDVRSALNALELAVLSADNDKDGYRHV
                     TLQDAKDCLQKGAFVSDKDGDMHYDVMSAFQKSIRGSDVNAALHYLARLIEAGDLPTI
                     VRRLLVISYEDIGLASPNAGQRTLAAIESAERLGLPEARIPLSQAVIELCLSPKSNSA
                     MSAIDSALSDIRNGHVGQIPNHLKDGHYQGAKDLGRSIGYKYPHQYVNGYVSQQYLPD
                     KLKNKIYYEPKT"
BASE COUNT          690 a          399 c          337 g          752 t
ORIGIN      
        1 ttatcttcat tttctttttt ctcttcgtta ttcgatcctg tatattcatt tatcttatct
       61 tttacatttt taacttgttc attatcgcta tttttaaatt tttctactgc gtctttagct
      121 ttatccataa aactcatatt aatcgctcct cttatatttg attagtttaa ttgaacttat
      181 tttttaagtt tatcaattgc atcagttatt ttgtttttag cattttcaac aacttctttt
      241 gctttaccag tcgctttatc ttgctgacct tctttttcta attctttgtt atcagtaacg
      301 ttacctactg tttctttaac atttccttta aattgatcga acttactttc gtctgccata
      361 gtgaaacctc cttggatgta tatatttata taccactaag gaggttcgct aaacattata
      421 atatgaagtt tttatgttat agtatagtat ttatacgatt aaatataaaa catgtatccg
      481 tctaaatctt cacttgtatc tacatattcc gctaaatatt tcaatgttgt attatctaaa
      541 acatctctca ctgcatctct catgcgaatc catagttgtt tttgcgcagg tggttctgat
      601 tcaatacttt caacaaatgt aattggacct tctaacagtc ttataatatc ccctgctgag
      661 atttcttccg ctggtactct taattggtat ccacctttag caccgcgtac acttcgaatt
      721 aaccccgcat ttcttaaagg acctacaagc tgttctaaat ataaatcact caaattattt
      781 tcttcagcaa ttgactttaa tgatatacat ccttgcccct cttttttagc aagagaaatc
      841 atcaatgtaa gtccatatct ccctttagta gaaattttca ttgtataacc tcacttaatt
      901 cgaatattga tattcccatt ttagcatttt ttgagttaag atagtataag aaaggtgtga
      961 caaatgtgag tacagaacca ttagcatcga gaatgcgccc aaaaaatata gatgaaatca
     1021 tttcccaaca acatttagtt ggaccaaaag gcattatcag aagaatggtt gacacaaaaa
     1081 aattaacttc aatgattttt tatggtccac ctggtatagg caaaacaagt attgccaaag
     1141 caatttcggg cagtacgcaa tataaattca gacaattgaa tgctgtaact aacactaaaa
     1201 aagatatgca acttgttgtt gaagaagcta aaatgtctgg tcaagttatc ttgttgttag
     1261 atgaaataca tcgactagat aaagctaaac aagacttttt attacctcat ttagaaaatg
     1321 gcaaaatcgt cttgattggt gctacaactt caaatcctta tcatgctatc aatccagcga
     1381 ttcgttcaag agcgcaaatt ttcgagttat atcctttaaa tgacgaagat gtgcgccaag
     1441 cgttaactcg tgcaatagaa gatgatgaga atggtttgaa aacatatcaa cccaaaattg
     1501 atgaagatgc catgacctac ttttctacac aaagccaagg tgatgttcgt agtgcgttaa
     1561 atgcattgga attagctgta ttaagcgcag ataatgacaa agacggttat cgacatgtta
     1621 cattgcaaga tgctaaagac tgtttacaaa aaggtgcatt tgtaagtgat aaggatggtg
     1681 acatgcatta cgatgttatg agcgctttcc aaaaatctat ccgtggtagc gacgtcaatg
     1741 ccgctttaca ttatttagca cgattaattg aagctggaga tttacctaca atagttcgac
     1801 gattacttgt aattagctat gaggatatag gcttagcctc acctaatgct ggtcagagaa
     1861 cacttgctgc tattgaatca gcagaacgtc taggtttacc agaagctaga attccactaa
     1921 gccaagcagt aatcgaacta tgcttatcac ctaagtcaaa ttcagcaatg agtgccattg
     1981 atagtgcatt gtccgatatt agaaacggtc atgtgggtca aattccaaat catttaaaag
     2041 atggacatta tcaaggtgct aaagatctag gccgatctat tggttacaaa tatccacacc
     2101 aatatgttaa tggctatgtt tcacagcaat atttacctga taaacttaaa aacaaaattt
     2161 attatgaacc aaaaacga
//
LOCUS       DAOBBH010000256         2177 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.256, whole genome shotgun sequence.
ACCESSION   DAOBBH010000256 DAOBBH010000000
VERSION     DAOBBH010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.256"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            191..382
                     /locus_tag="P6L01_002506"
     CDS             191..382
                     /locus_tag="P6L01_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031543.1"
                     /translation="MRKEIEALIFSDVSSYDIYVNTGVNQGLIGDIKDGYLTIDSMPY
                     IDAERLYHFAMERKSLVTN"
     gene            complement(521..706)
                     /locus_tag="P6L01_002507"
     CDS             complement(521..706)
                     /locus_tag="P6L01_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031544.1"
                     /translation="MLYILITALNVILNKLKMKDSNIISFIYSRHDRILYVNLVGNRF
                     KNNISMTKATSNIGRGF"
     gene            complement(642..923)
                     /locus_tag="P6L01_002508"
     CDS             complement(642..923)
                     /locus_tag="P6L01_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDP3031545.1"
                     /translation="MTYHERVLALRAESKRTAFDFRFEDLFSKEEWLSMSLAERQKAE
                     KAFRHEVKNMDDVRMPFSSVHDAQVKLYNVVYSYNGIKRNFKQVENEGF"
     gene            1212..1907
                     /locus_tag="P6L01_002509"
     CDS             1212..1907
                     /locus_tag="P6L01_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDP3031546.1"
                     /translation="MTVLTDKIAVVTGAGSGIGEAIATLLHEEGAKVVLAGRNKDKLQ
                     NVANQLAQDSVKVVPTDVTNKEEVDELMKIAQQTFGGLDIVINSAGQMLSSKITDYQV
                     DEWDSMIDVNIKGTLYTAQAALPTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAV
                     HTITQGLEKELAKTGVKVTSISPGMVDTAITAAYNPSDRKKLDPQDIAEAVLYALTQP
                     KHVNVNEITVRPV"
BASE COUNT          763 a          315 c          391 g          708 t
ORIGIN      
        1 tctgacttat taaactttta tataattaac tatttcttaa ttttccaaaa tagtgataaa
       61 tttgtgaaat acatcacaaa cctctttatt tatttggaaa ttcatgtaat attagacttg
      121 taagaagtta ataaatagag agagacgaga gagtttatat aaataatata taaacattgg
      181 agtgatgatt atgagaaaag agattgaagc gcttattttc tcagacgtaa gtagctatga
      241 tatttacgtg aacactggtg taaatcaagg attaattggt gacatcaaag atggttacct
      301 aactattgat tctatgcctt acattgatgc tgagcgtttg tatcactttg ctatggaacg
      361 taaatcgtta gtcactaact agttcttatt gccaatgatt actaccccta gtcggcggca
      421 attgaagtgt gattgatgta acttgccctc gttggtgagc aattgagggc agatcccttt
      481 aattaagtaa accctaactc cccacaaatc tggaacgata ctaaaagcca cgtcctatat
      541 tggatgtggc tttagtcata cttatattat ttttaaaacg attacctaca agatttacat
      601 ataaaattct atcatgtctg ctatatataa acgaaatgat attagaatcc ttcattttca
      661 acttgtttaa aattacgttt aatgccgtta taagaatata caacattata taattttact
      721 tgggcgtcat ggacacttga gaagggcatt cttacatcgt ccatattttt aacttcgtgt
      781 cgaaatgctt tttcagcttt ttgtctttct gcaagagaca tacttagcca ttcttctttg
      841 ctaaataaat cttcgaatcg aaaatcaaat gcggttcttt tactttctgc tcttaatgct
      901 aaaacacgct catgatatgt cacgtttaat tcctccctct aatcattcaa tcgaatgata
      961 aatcacagta ttaactttag tactaatata atgtttttac aataaagaag tgacgatttt
     1021 tcaacatttt taaaaataaa aaatataagc aacgattaat tgtatatttc gaatggcttg
     1081 taatgtaatt tatctatgaa aatgaagcga gttattatga ccgttatatg aattgtgata
     1141 cgctttttaa aagtaatatg tatttatgat ttaccctgaa aaaataaaaa gaatcaaagg
     1201 agtcgatcat tatgacagta ttaacagata aaatagcagt agttacaggt gcaggtagtg
     1261 gtattggaga agcaattgca acattactac atgaagaagg ggcgaaagtt gtcttagccg
     1321 gtagaaataa agataaacta caaaatgtgg cgaatcaatt agctcaagat agtgtgaagg
     1381 tagtgccaac agatgtaacg aataaagaag aagtcgatga attgatgaaa attgcacaac
     1441 aaacattcgg tggtttggat attgttatca atagtgcggg acaaatgtta tcgtctaaga
     1501 ttactgatta tcaagtagat gagtgggata gtatgattga tgtgaatatc aaaggcactt
     1561 tatatacggc acaggctgca ttaccaacta tgttagaaca atcaagtgga catcttatta
     1621 acattgcatc tatttctggc tttgaagtaa cgaaaagtag tacgatttat agtgcgacga
     1681 aagcagcagt tcacactatt actcaaggat tagaaaaaga gttggcaaag acaggcgtta
     1741 aagtaacaag catttctcca ggaatggtag atacagcaat aacagccgca tataatccaa
     1801 gtgatcgtaa aaagcttgat ccacaagata ttgcagaagc ggtattgtat gcattaacac
     1861 aaccaaagca tgttaacgtg aatgaaatta cagtgcgtcc tgtataaaaa ggtgtttgag
     1921 gtgtgggaga tagacattta atgtaatagc tatcgatttt gttaatgtta gtaaggcaat
     1981 aatgacatga gggcataata ggcaattatt ccttgtacaa ttctcagtaa ttggtgattt
     2041 taacaatcaa gttaatgaag gcgacgatgt tagtgttatg gtttataatc tataatgttc
     2101 gtaattacag attacaaaaa aatttcattt tccaccacat tttaggagtt actttaatga
     2161 acaaaaaaca tgttttt
//
LOCUS       DAOBBH010000257         2174 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.257, whole genome shotgun sequence.
ACCESSION   DAOBBH010000257 DAOBBH010000000
VERSION     DAOBBH010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.257"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            59..1615
                     /locus_tag="P6L01_002510"
     CDS             59..1615
                     /locus_tag="P6L01_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDP3031547.1"
                     /translation="MENTINESEKKKRFKLKMPGAFMILFILTVVAVIATWVIPAGAY
                     SKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPNTY
                     ERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKT
                     KGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASS
                     VGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKKIKNDPK
                     ASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWFPVM
                     ASAFLIFTIIIMFIAGTGKSGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVLNEG
                     MISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLADTV
                     GIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIFGGG
                     VLITQVLIYS"
     gene            1922..2149
                     /locus_tag="P6L01_002511"
     CDS             1922..2149
                     /locus_tag="P6L01_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031548.1"
                     /translation="MTHLTKVLDTLTGICVVLLFSKYFVAYANMVFDWNLRWYLLENI
                     PHLPIILFILMFIFGVPSEMIKDRQRKNNGV"
BASE COUNT          687 a          295 c          428 g          764 t
ORIGIN      
        1 atatttataa aatctttaag gtagaaatgc taggttaaat gtcgaaggag atgaaaccgt
       61 ggaaaataca attaatgaaa gtgaaaagaa aaaacgattt aaattaaaaa tgccaggtgc
      121 atttatgatt ttattcattt taacggttgt tgcagttata gcaacatggg ttattcctgc
      181 tggtgcatat tctaaacttt cttacgaacc ttcatcccaa gaactaaaga tagttaaccc
      241 tcataaccaa gtgaaaaagg ttccgggtac gcaacaggaa ctagacaaaa tgggggttaa
      301 aattaagatt gaacaattta aatcaggtgc aattaataag ccagtatcaa ttccgaatac
      361 ttatgaaaga ttaaagcaac atccagctgg accagaacaa ataacaagta gcatggttga
      421 aggtacgata gaagcggtcg atatcatggt attcattctt gtactagggg gacttattgg
      481 cgtagttcaa gccagtggtt cttttgaatc gggattgtta gctttaacga agaaaacaaa
      541 agggcatgaa tttatgctaa ttgtgtttgt atcaatacta atgattatcg gcgggacgtt
      601 atgtggtatt gaagaagaag ctgtagcatt ctatccgatt ttagtcccta tatttatagc
      661 gttaggatac gattctatcg tttcagttgg tgccatattc cttgccagct ctgtcggtag
      721 tacattttca actattaacc cgttctcggt tgtaattgcc tctaatgccg ctggtacaac
      781 ttttacggat ggcttgtatt ggagaatagg tgcttgtatt gtcggtgcga tttttgttat
      841 tagttattta tattggtact gtaaaaaaat taaaaacgat cctaaagcgt catattctta
      901 tgaagacaaa gatgcttttg aacagcaatg gtctgtatta aaagatgacg atagtgccca
      961 ttttactttg cgtaagaaga taatccttac attatttgta ctaccatttc caattatggt
     1021 atggggagtt atgacgcaag gttggtggtt cccagttatg gcttcagcat ttttaatatt
     1081 tacaattata ataatgttta ttgctgggac aggtaaatct ggattgggag aaaaaggaac
     1141 tgtagatgca tttgtcaatg gtgcatcaag tttagtaggt gtatctttaa ttattggttt
     1201 agctcgaggt attaatttag tgttgaatga aggtatgatt tcagatacaa tcttacactt
     1261 ttcatcatct ttagttcaac atatgagtgg accattattt atcatcgtat tactatttat
     1321 tttcttctgt ttaggtttta tcgtgccatc atcttctgga ttagcggtat tatcaatgcc
     1381 tatctttgca ccactagctg atacagtagg tataccaaga ttcgtcatcg ttacgacata
     1441 tcaatttggt caatatgcga tgttattctt agcgccgact ggacttgtta tggccacact
     1501 acaaatgtta aacatgcgat attcacattg gttccgattt gtatggccgg tagttgcttt
     1561 tgtattgatt ttcggtggcg gagtactaat tacgcaagta ctaatttatt cataatttga
     1621 aatgctatat tataaaaata ctaattgggt tttatgcatc tcgtaggttt gtagaaatac
     1681 taaactaagc gaggtgcatt attatttttg attaagaaaa taatgacggt aatgataaca
     1741 ctagtaagta attgatacaa tgctctattt aataatgata tttttaaaat ttgtttttaa
     1801 tgtaatgtta gatctatggt atattatatt tatcgtggta aatatgtatt tgctgtaaac
     1861 agtttttgta acacatgaca catatggttg tgaaaatagc atatataaag gatggctata
     1921 aatgacacat ttgacaaagg ttttagatac actaactgga atatgcgtag tattattatt
     1981 tagtaaatat tttgtggcgt atgcaaatat ggtgtttgat tggaatttaa gatggtattt
     2041 gctagaaaac ataccacatt tgccaattat attatttatt ctgatgttta ttttcggagt
     2101 accttctgaa atgataaaag ataggcaaag gaaaaataac ggtgtttaat ttatcgatat
     2161 ttagaggtga taaa
//
LOCUS       DAOBBH010000258         2172 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.258, whole genome shotgun sequence.
ACCESSION   DAOBBH010000258 DAOBBH010000000
VERSION     DAOBBH010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.258"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..890
                     /locus_tag="P6L01_002512"
     CDS             <1..890
                     /locus_tag="P6L01_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500380.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDP3031549.1"
                     /translation="NKFGQKIFKVALDGGFDCPNRDGTVAHGGCTFCSAAGSGDFAGN
                     RADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTHAPVEVLKEKFEPVLKEPGVVGL
                     SIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIHQSTSDLINRAHDMKTYYDGVAK
                     LRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDVQGIKIHLLHLLKGTPMVKQYDK
                     GLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGPIDIMVGPMWSVNKWEVLNGIDA
                     ELARRNSYQGLRYKSKVKQ"
     gene            887..1450
                     /locus_tag="P6L01_002513"
     CDS             887..1450
                     /locus_tag="P6L01_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDP3031550.1"
                     /translation="MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPE
                     GHVYGFDIQDLALENTRDKVKDFNHVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYL
                     PKGDKSIVTKPDTTIQAINSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQ
                     KHAQVLQYQFLNQRNHAPFICAIEKIS"
     gene            complement(1573..1974)
                     /locus_tag="P6L01_002514"
     CDS             complement(1573..1974)
                     /locus_tag="P6L01_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDP3031551.1"
                     /translation="MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILL
                     TLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQ
                     QEKVELLNFISDAIASRATAMQNSLNAIIAV"
BASE COUNT          756 a          366 c          361 g          689 t
ORIGIN      
        1 aaaataaatt tggacaaaaa atatttaaag ttgcattgga tggcgggttt gactgtccta
       61 accgcgatgg cactgtagca catggtggat gtacattttg ttctgctgca ggtagcgggg
      121 actttgcagg taatcgtgca gattcaatcg cagtacaatt taaagaaatt aaggaaaaga
      181 tgcatgagaa atggcacgaa ggaaaatata ttgcttattt tcaggcattt acaaatacac
      241 atgcaccagt tgaagtatta aaagaaaaat tcgaacctgt acttaaagaa ccgggtgttg
      301 taggattatc tattggtacg cgtcctgact gtctaccaga cgatgttgtc gaatatttag
      361 cagatttgaa tcaacgaaca tacttatggg ttgaattagg actacaaaca atccatcagt
      421 caacatctga tttaatcaat cgtgcccatg atatgaaaac ttattatgat ggtgtggcaa
      481 aattacgtaa gcataatatc aatgtatgta cacacatcat taatggctta cctggcgaag
      541 actatgacat gatgatggct actgccaaag aagttgcaca aatggatgta caaggtatta
      601 aaattcattt acttcatttg ttaaaaggta caccgatggt aaaacaatac gataaaggtt
      661 tattaacttt tatgactcaa gaagagtaca caaacctagt tgtggaccaa ttagaagtga
      721 ttccccctga aatgatcgtt caccgaatta ccggtgatgg tccaatagat atcatggtag
      781 gtccaatgtg gagtgttaat aaatgggaag tattaaatgg catcgatgct gaattagcac
      841 gtagaaattc ttatcaaggc ttgcgttaca agtctaaggt gaagcaatga aattagaacg
      901 tatactccct ttttcaaaaa cacttattaa acaacatata acaccagaaa gtattgttgt
      961 ggacgcaact tgcggtaacg gcaatgacac tttattttta gccgaacaag taccagaagg
     1021 acatgtttat ggtttcgaca ttcaagattt agctttggaa aatacacgtg ataaagttaa
     1081 ggatttcaat catgtttctt taataaaaga tggacatgaa aatattgaac atcatataaa
     1141 tgatgcacat aaaggtcata ttgatgcagc catctttaac ctaggttatt tgcctaaagg
     1201 tgataaatct atcgtgacaa agcctgacac gacaatccaa gctattaatt cattgctatc
     1261 attaatgtca attgaaggta ttattgtact tgttatatat catggtcata gcgaaggaca
     1321 aattgagaag catgcattgc ttgattactt gagtactttg gatcaaaagc atgcgcaagt
     1381 tttgcaatat caatttttaa accaacgtaa tcatgctcca ttcatttgtg ccatagaaaa
     1441 aatttcttaa gcatttgctg cgctatacta tttataattt aggattgagt agtcaacatt
     1501 tgaaaaatta ataccaacaa caacaaaaag aggttttcaa cttaatgaaa acctcttttt
     1561 atgctattaa acttacacag caataattgc gtttaaacta ttttgcattg ctgttgctct
     1621 acttgcaatg gcatcactga tgaaattcaa caactctact ttctcttgtt gtaacttttc
     1681 attgaaatga ataataactg ttctttcatc gtcaacagga cgctctttgt aaatccattc
     1741 taattcaact aaattattat acgttctcgt acgcttatac ggtttaactt caacaaatct
     1801 gtccatttct ttaagcgtca tagaaccttt ttgccataaa gttagtaaaa ttaaaatttc
     1861 ttctctagac attttgtatt cgctttcaat ctcgctgaaa attgagttaa tgtcacccaa
     1921 aagtgtttct aattgcaaaa tcccaaatac agtgtcgtta tttacttttt tcatgctaaa
     1981 catctcccaa ttaataatca catatttagc taccatacaa gtattaacaa acatcccaac
     2041 aatcccaaaa cttgtatgtg ctaacttatg cataaaacta caagtgtaaa taaacttgct
     2101 ttctattcaa ttgtcaaagt tgaataaaat taaataagta taaaaaccaa taatcaatag
     2161 agttaatatt ta
//
LOCUS       DAOBBH010000259         2154 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.259, whole genome shotgun sequence.
ACCESSION   DAOBBH010000259 DAOBBH010000000
VERSION     DAOBBH010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.259"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..541)
                     /gene="recU"
                     /locus_tag="P6L01_002515"
     CDS             complement(<1..541)
                     /gene="recU"
                     /locus_tag="P6L01_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDP3031552.1"
                     /translation="MLCIINNVYYQTCVVKFMNYPNGKPYRKNSAIDGGKKTAAFSNI
                     EYGGRGMSLEKDIEHSNTFYLKSDIAVIHKKPTPVQIVNVNYPKRSKAVINEAYFRTP
                     STTDYNGVYQGYYIDFEAKETKNKTSFPLNNIHDHQVEHMKNAYQQKGIVFLMIRFKT
                     LDEVYLLPYSKFEVFWKRYK"
     gene            796..975
                     /locus_tag="P6L01_002516"
     CDS             796..975
                     /locus_tag="P6L01_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001788819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031553.1"
                     /translation="MLLISRGVFDLNDFIRLKVFSSYNNIHDKILYLYRKFLDLLDLT
                     DNTFEKIVKSNYIEC"
     gene            1030..1380
                     /locus_tag="P6L01_002517"
     CDS             1030..1380
                     /locus_tag="P6L01_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YppE family protein"
                     /protein_id="HDP3031554.1"
                     /translation="MNDVVESLIYEVNNMQQNFENVKSQQQDHDFYQTVKPYTEHIDS
                     MLNEIKLHREFIIEVPYMNSRKFELLIANIEQLSVECHFKRTSRKLFIEKLKSVQYDL
                     QNILDGVTKEGTYG"
     gene            1373..1936
                     /locus_tag="P6L01_002518"
     CDS             1373..1936
                     /locus_tag="P6L01_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDP3031555.1"
                     /translation="MVKTVYVTGYKSFELNIFKDDAPEVHYLKQFIKHKIEQLLDEGL
                     EWVLIQGQMGIELWTAEVVIELQRTYDSLKFAVITPFQGHTEKWNEHNQSKYANIIKH
                     ADYVDSIFHTSYQGPFQFKQADQFMLEHSDQTLLIYDEEQEASPKFFKQMLVDFMDKT
                     NYTCDIVTFDELTAFINDLQWSEDQSF"
     gene            1950..>2154
                     /locus_tag="P6L01_002519"
     CDS             1950..>2154
                     /locus_tag="P6L01_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDP3031556.1"
                     /translation="MSDVSLKLSAKDIYEKDFEKTMARGYRREEVDAFLDDIIADYQK
                     MADMNNEVVKLSEENHKLKKELEE"
BASE COUNT          806 a          284 c          348 g          716 t
ORIGIN      
        1 ctttatatct cttccaaaat acttcgaatt ttgaataggg taaaagataa acttcatcta
       61 gcgttttaaa acgaatcatt aaaaacacaa tacctttttg ttgatatgca tttttcatat
      121 gttcgacttg atggtcatga atattattta aaggaaagga cgtcttgttt ttagtttcct
      181 ttgcttcaaa atcaatataa taaccttgat aaacgccgtt gtaatcagtt gttgaaggtg
      241 tacgaaaata agcttcgtta atcacagctt tactccgctt aggatagttg acattaacta
      301 tttgtactgg cgtaggcttt ttgtgaataa ctgcaatgtc gcttttaaga taaaacgtat
      361 ttgaatgttc gatatctttt tcaagtgaca taccacgtcc accatactca atattactaa
      421 aggcagcggt ctttttccct ccgtctatag cactattttt acgatatggt ttaccatttg
      481 gataattcat aaatttcacc acacaagttt ggtagtaaac gttgtttatt atacacaaca
      541 ttatagtgaa acaccaaact taaattagta taaatgcgaa agacacaata caccaaagct
      601 tattttaacg tgatttcagc attaacacaa taatattata atacatttac cgttaatgtt
      661 ggaattttac acacaatttt aattaattga aaagttttgc ttataaaaat gaatactcat
      721 tacaatattt ttgaattttg ttataaaagt caaatcatga tcagtaattt ttatttttga
      781 ggcaagtaca agtatgtgtt gttaatcagc agaggtgtat ttgatttaaa tgattttatt
      841 agattaaaag tattttcatc gtataacaac atacacgata aaattttgta tttatatagg
      901 aagtttttag atttacttga tttaacagat aacacatttg aaaaaatagt taaatcaaat
      961 tatatagagt gttaaaacat gacgaataca gtatattgtg ataaattaaa aaatgtagga
     1021 ggcgtcctca tgaatgatgt agtcgaatca ctaatttatg aagttaacaa catgcaacaa
     1081 aattttgaaa atgtgaaatc acaacaacaa gatcatgatt tttaccaaac tgtaaagcca
     1141 tatactgaac atattgatag catgctcaat gagatcaaat tacatcgtga atttattata
     1201 gaagtacctt atatgaattc aaggaaattt gagctactga ttgctaacat tgaacaactt
     1261 tctgtcgaat gtcattttaa gcgaacaagt cgaaagttat ttatagaaaa gcttaaaagt
     1321 gtccaatatg atttacaaaa tatattagat ggcgtaacaa aagagggtac ttatggttaa
     1381 aacagtttat gtaacaggtt acaaatcatt cgaattaaac atttttaaag atgacgcacc
     1441 tgaagtacat tatttaaaac aatttataaa acataaaatt gaacaactgt tggatgaagg
     1501 attagaatgg gtgttaatac aagggcaaat gggtatagaa ttatggactg cagaagttgt
     1561 tattgaatta caacgaacat atgattcgtt aaagtttgct gtaattacac cgttccaagg
     1621 acatacagaa aagtggaatg aacataatca aagtaagtat gccaacataa ttaagcatgc
     1681 agattatgtt gatagtattt ttcatacgtc gtatcaaggt ccttttcaat ttaaacaagc
     1741 agatcaattt atgcttgaac attcggatca aacattgctc atttatgatg aggaacaaga
     1801 ggcaagtcct aagttcttca agcagatgtt agttgatttt atggataaaa caaactatac
     1861 ttgtgatatt gtgacgttcg atgaattaac agcattcatc aatgacttac agtggtctga
     1921 agatcaaagt ttctaatgag gtggaaaaaa tgtcagatgt ttcattgaaa ttatcagcaa
     1981 aagatattta tgaaaaagat tttgaaaaaa cgatggctcg tggctataga agagaagaag
     2041 tagatgcatt tttagatgac attattgctg attatcaaaa aatggccgat atgaataatg
     2101 aagttgtaaa attatcagaa gagaatcata aacttaaaaa agaattagaa gaat
//
LOCUS       DAOBBH010000260         2154 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.260, whole genome shotgun sequence.
ACCESSION   DAOBBH010000260 DAOBBH010000000
VERSION     DAOBBH010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.260"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..2058)
                     /locus_tag="P6L01_002520"
     CDS             complement(<1..2058)
                     /locus_tag="P6L01_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001799555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDP3031557.1"
                     /translation="MGERIKGLSIGLDLDAANLNRSFAEIKRNFKTLNSDLKLTGNNF
                     KYTEKSTHSYKQRIKELDGTITGYKKNVDDLAKQYGKVSQEQGENSAEAQKLRQEYNK
                     QANELNFLEKELEKTTTEFEEFKKAQVEAQRMAESGWGKTSKVFESMGPKLTKMGDGL
                     KSIGKGLMIGVTAPVLGIAAASGKAFAEVDKGLDTVTQATGATGGELKKLQNSFKDVY
                     GNFPADAETVGGVLGEVNTRLGFTGKELESATESFLKFSHITGSEGVQAVQLITRAMG
                     DAGIEADEYQSVLDMVAKAAQASGISVDTLADSITKYGAPMRAMGFEMKESIALFSQW
                     EKSGVNTEIAFSGLKKAISNWGKAGKNPREEFKKTLAEIEKTPDIASATSLAIEAFGA
                     KAGPDLADAIKGGRFSYQEFLKTIEDSQGTVNQTFKDSESGSERFKVAMNKLKLVGAD
                     VWTSIESAFAPVMEELIKKLSIAVDWFSNLSDGSKRSIVIFGGIAAAIGPVVFGLGAF
                     ISTIGNAVTVLAPLLAGIAKADGLISFLSTKVPILGTVFTALTGPIGIVLGVLAGLAV
                     AFTIAYKKSETFRNFVNGAIESVKQTFSNFIQFIQPFIDSVKNIFKQAISAIVDFAKD
                     IWSQINGFFNENGISIVQALQNICNFIKAIFEFIINFVIKPIMFAIWQVMQFIWPAVK
                     ALIV"
BASE COUNT          649 a          460 c          296 g          749 t
ORIGIN      
        1 gacaattaaa gctttaaccg ccggccaaat aaattgcatc acttgccaaa tcgcgaacat
       61 gattggttta attacaaaat ttataataaa ttcaaatata gctttgataa aattgcatat
      121 attttgaagc gcttgaacaa tagaaattcc gttttcatta aagaatccat taatttgact
      181 ccaaatatct ttagcaaaat caactattgc tgatatcgct tgtttaaaga tgtttttaac
      241 agaatcaatg aaaggttgaa taaattgaat aaaattacta aatgtttgtt taacactttc
      301 aattgcacca ttaacaaaat ttctgaaagt ttcagatttc ttataagcaa ttgtaaatgc
      361 gactgctaag ccagccaaaa cacctaatac aatgccaatt ggaccagtta aagccgtgaa
      421 gacagttcct aatataggta ctttagtcga taaaaaacta attaatccat cagcctttgc
      481 aataccagct aatagtgggg ctaatacagt tactgcattg ccaattgtac ttataaatgc
      541 gcctaatcca aaaactacag gaccaattgc agcagcaata ccaccgaaaa taacaattga
      601 tcttttagaa ccatcactta aattggaaaa ccaatcaacc gctatagata gctttttgat
      661 taattcttcc attactggtg caaacgcact ttcaatagaa gtccatacat cagcacctac
      721 taattttaat ttattcattg ctactttaaa tctttcggag ccactttcag aatctttaaa
      781 cgtttgattt actgtgcctt gggaatcttc gatagttttt aaaaattctt gataactaaa
      841 acgaccacct ttaatagcat ctgctaaatc aggacctgct tttgcaccaa atgcttcaat
      901 cgctaaactt gttgcgctag ctatatccgg cgtcttttca atttctgcta atgtcttctt
      961 aaattcttct cttggatttt taccagcttt accccaattg gatatagctt ttttcaaacc
     1021 actgaaggct atttcagtat taacacctga tttctcccat tgagagaata aagcgattga
     1081 ttctttcatc tcaaagccca tagccctcat tggagcaccg tatttagtaa tgctatcagc
     1141 taatgtatca acacttatac cgctagcctg tgctgctttc gctaccatat caagtacact
     1201 ttgatactca tcagcttcaa tacctgcatc acccattgca cgcgtaatta attgaacggc
     1261 ttgtacgcct tcagaacctg ttatgtgact aaatttcaag aatgactctg tggcactctc
     1321 aagttctttg ccagtgaaac ctaaccttgt gttaacttcc cctaaaacac cgcctacagt
     1381 ctcagcatct gctggaaagt tgccataaac atctttaaat gaattctgca acttcttaag
     1441 ctctccgccg gttgctcctg ttgcttgggt aactgtatct aaacctttat caacttctgc
     1501 aaaagctttt cctgatgctg ctgcaatacc taaaacaggt gcggtaacac caatcatcaa
     1561 acctttacca atggatttta aaccatcacc catttttgtt aatttaggtc ccatactttc
     1621 aaaaacttta ctggtttttc cccagccact ttctgccatt ctttgagctt caacttgagc
     1681 ttttttgaac tcttcaaact cagttgttgt tttttctagt tctttttcta aaaaattcag
     1741 ctcatttgct tgtttgttat attcttgtcg taatttttga gcttccgcgc tgttttcgcc
     1801 ctgttcttga gataccttgc catattgctt ggctaaatca tcaacgtttt tcttataacc
     1861 tgtgatagtt ccatcaagtt ctttaatcct ttgtttgtaa ctatgagttg atttttcggt
     1921 atatttgaag ttgttaccgg ttaactttaa gtcagaattt aaagttttaa agtttcgttt
     1981 gatttctgca aatgatctat ttaaatttgc tgcatctaaa tccaaaccta tagataaacc
     2041 ttttattctt tctcccattt tttacctcct ttctaaaaaa gttcaaaaaa ataaccctaa
     2101 ccaaacggtt aaggttaaaa cgcatcaatt aaagcctctg ctttttcttc agaa
//
LOCUS       DAOBBH010000261         2151 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.261, whole genome shotgun sequence.
ACCESSION   DAOBBH010000261 DAOBBH010000000
VERSION     DAOBBH010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.261"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            126..334
                     /locus_tag="P6L01_002521"
                     /pseudo
     CDS             126..334
                     /locus_tag="P6L01_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(352..753)
                     /locus_tag="P6L01_002522"
     CDS             complement(352..753)
                     /locus_tag="P6L01_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HDP3031558.1"
                     /translation="MTSYEEETDYRNVPRELLDNNIPMGRGMIKWAPFATLPEQFETI
                     QQYIIDQNKITRPVLSDDQLAELNIRLHEALQYAQPVEVKFYNNGFVDSVRLTIYRID
                     AINYEIDGYVYNQQQRQKISIFDILDIVLLP"
     gene            1023..2051
                     /locus_tag="P6L01_002523"
     CDS             1023..2051
                     /locus_tag="P6L01_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDP3031559.1"
                     /translation="MTNGYIGSYTKKNGKGIYRFELNENQSRIDLLEIGFELEASTYL
                     VRNNEVLYGINKEGEQCGVASLKIDDNGELHLLNKCLSSKAGTGCYVSISEDKRYLFE
                     AVYGAGIIRMYELNTHTGEIIRLIQELAHDFPTGTHERQDHPHAHYINQTPDGKYVAV
                     TDLGADRIVTYKFDDNGFEFYKESLFKDSDGTRHIEFHDNGKFAYVVHELSNTVSVAE
                     YNDGKFEELERHLTIPENFDGDTKLAAVRLSHDQQFLYVSNRGHDSIAIFKVLDNGQH
                     LELVTITESGGQFPRDFNIASSDDLLVCAHEQGDSVVTVFERSKETGKITLCDNTRVA
                     SEGVCVIF"
BASE COUNT          768 a          302 c          374 g          707 t
ORIGIN      
        1 tttaaaaaat aagacacttt gcccaacttg cacataaatg taaaattcaa taaaatgaat
       61 tttctgtgtt gggtcccttc ttataattta ataaatacca ctaaactaaa ttaacgaggt
      121 gccttatgta taaaaattat aacatgaccc aactacacta ccaatagaaa cttctgttag
      181 aatccctcaa aatgatattt cacgatatgt taatgaaatt gttgaaacaa tacctgatag
      241 cgaattcgat gaattcagac atcatcgtgg cgcaacatcc tatcatccaa aaatgatgtt
      301 aaaaatcatc ttatatgcat atactcaatc tgtttaatta tgttcaaagc attaaggtaa
      361 caagacaata tctaagatat caaagataga aattttttga cgttgttgct gattgtaaac
      421 ataaccatct atttcataat taatagcatc aatacgataa atggttaagc gtactgaatc
      481 tacaaagcca ttattataaa atttaacttc tacaggttgg gcatattgta gcgcctcgtg
      541 tagccgaatg tttagctcag ccaattgatc atctgataat acaggacgtg taattttgtt
      601 ttggtcgata atgtattgtt gaatcgtttc gaattgttcg ggtaatgttg caaaaggagc
      661 ccatttaatc atgcctcttc ccataggtat attgttatct agtaattctc ttggaacgtt
      721 acgataatca gtttcttctt cataacttgt catccttaat tcaccccaat ctgataatta
      781 cattatacga acatgtgttc tgttttgcaa caaaaatttt gtggaagcat aaacgcgtta
      841 ataattaatg ctcgtgtaag taaaaaagag ggattaatta aaatcgaata atgacatatc
      901 acagcaaata gttcttttaa agtagttaaa cagttttagc gttaaggaaa tgataaatga
      961 ttgttaattc tagctaaaat ttaataaaat gaaaataaga ctgacatgga ggggtaaaag
     1021 taatgacaaa tggatatatt ggttcttaca ctaaaaagaa tggtaaaggg atttatcgtt
     1081 ttgaattaaa cgaaaatcag tcacgtattg atttattaga aataggattt gaattagaag
     1141 cgtctacata tttggtgcgt aataacgaag ttttatatgg aatcaacaaa gaaggagaac
     1201 aatgcggtgt tgcgagtttg aaaattgatg acaatggtga attgcattta ttaaataaat
     1261 gtttgtcttc aaaagctggt acaggttgtt atgtatcgat ttcagaagat aaacgatatt
     1321 tatttgaagc ggtatatggt gctggcatca tacgtatgta tgaattaaat acgcacacag
     1381 gtgaaattat acgtctaatt caagaacttg cacatgattt tccaacaggt acacatgaaa
     1441 gacaagatca tccacacgca cattatatta atcaaactcc agatggtaag tacgttgcag
     1501 taacagattt aggtgctgat cgtatcgtta cttataaatt tgatgacaac gggtttgaat
     1561 tttataaaga atctttattt aaagatagtg atgggacaag acatattgaa tttcatgata
     1621 atggaaaatt tgcttatgtc gtacacgaat tatcaaatac tgtgagtgtt gcagaatata
     1681 atgacggtaa atttgaagag ctcgagcgtc atttaacaat tcctgaaaac tttgatggag
     1741 atactaaact tgcagcagtg cgtttgtctc atgatcaaca attcttatat gtatctaata
     1801 gagggcatga tagcattgca atttttaaag ttcttgataa tggtcaacac ttagaactag
     1861 taacaattac tgaaagtggt ggtcaattcc caagagattt taatattgcc tcatcagatg
     1921 accttttagt ttgtgctcat gagcaaggag attcagttgt aactgttttt gaaagaagta
     1981 aagaaacagg taaaattact ctatgtgata atactcgtgt agcatctgaa ggtgtatgtg
     2041 tcatatttta atctttaatt aatcatgata aaaagaaaac catgtttaca aaaaatttgt
     2101 gtataccttg aaatttattg ttttcaagaa catcaaatta tggaagcatg g
//
LOCUS       DAOBBH010000262         2081 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.262, whole genome shotgun sequence.
ACCESSION   DAOBBH010000262 DAOBBH010000000
VERSION     DAOBBH010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.262"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            501..1649
                     /locus_tag="P6L01_002524"
     CDS             501..1649
                     /locus_tag="P6L01_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001806960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDP3031560.1"
                     /translation="MRLNKNESELKLCRDLITEFETLSKYNNITNFEVDYKKYVQDVN
                     QHQELSKEIEDKLMQLSQRKLIEQNNLNHYENQLETYNNDLELNEQSIEMEMSRLNLT
                     DDNDINEIIAWRGEQEELEQKRDTYKKRYHEFEMEIARLESLTKDKELLDSDKLKDEY
                     EQKKEKMNTLIDEYSAVHYQCQNNINKTQSIVSHINYLNQELKDQQEIFQLAEIVSGK
                     NNKNLTLENFVLIYYLDQIIAQANLRLATMSDNRYQLIRREAVSHGLSGLEIDVFDLH
                     SNKSRHISSLSGGETFQSSLALALGLSEIVQQQSGGISLESIFIDEGFGTLDQETLET
                     ALDTLLNLKSTGRMVGIISHVSELKNRIPLVLEVKSDQYQSSTRFKRN"
     gene            complement(1720..>2081)
                     /gene="mscL"
                     /locus_tag="P6L01_002525"
     CDS             complement(1720..>2081)
                     /gene="mscL"
                     /locus_tag="P6L01_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDP3031561.1"
                     /translation="LKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIGK
                     IFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVVE
                     ENVVLLTEIRDLLREKK"
BASE COUNT          842 a          280 c          338 g          621 t
ORIGIN      
        1 ataaactatt aggtagtaag caagagttag agaatcaaat taatgaagaa acaactataa
       61 tgaagaatct cgaaataaaa ttagatcact acgataaatc aaaattggac ttaaatgata
      121 aagaaagctt tataagtgaa attaaatctg ctgtaaagat tggagatcaa tgtccgatat
      181 gtggtaatga aattcaggat ttagggcatc atattgattt tgacagtatt gctaaacgtc
      241 aaaatgaaat taaagaaatt gaagcaaata tccacgcaat aaaatcgaat attgctgtgc
      301 ataattctga aattaaattt gttaatgaaa aaatatcgaa tattaatatt aaaacgcaaa
      361 gtgatttttc actcgaagta ttgaataagc gtctgctaga aaatgaaaat gcattgaata
      421 atcaaagaga tcttaataaa tttatagaac aaatgaaaga agaaaaagat aatctaacgt
      481 tgcaaattca taataaacaa ttgcgtctaa ataaaaatga atctgagttg aaattatgtc
      541 gagatctcat cactgaattt gaaacactct cgaaatataa taatatcact aattttgagg
      601 tggattataa gaagtatgtt caagatgtga atcagcatca agaactctca aaggagattg
      661 aagataagtt aatgcaattg tctcaaagaa agttaattga gcaaaataat ctaaatcact
      721 atgaaaatca actagaaact tacaataatg acttagaatt gaatgaacaa tctattgaaa
      781 tggaaatgtc gaggctgaat ttaactgacg acaatgatat aaatgaaata atagcctgga
      841 ggggcgagca agaggaatta gagcagaaaa gggatactta taaaaaacgt tatcatgaat
      901 ttgaaatgga aatagctagg ttagaatcat taaccaagga taaagagtta ttggactctg
      961 ataaattaaa agatgaatat gagcaaaaaa aagaaaagat gaatacactg atagatgaat
     1021 actctgctgt tcattatcaa tgtcaaaata atattaataa aacacaatct atagtttcgc
     1081 atattaatta cttaaatcaa gaattaaagg atcaacaaga aatttttcaa ttggctgaaa
     1141 ttgtcagtgg taagaataac aaaaatctta cattggaaaa ctttgtctta atttactatt
     1201 tagatcaaat tattgcccaa gcaaatctga gattagcaac aatgtcagat aatcgatacc
     1261 aactaattag gcgagaagcg gtttctcatg gtcttagtgg cctagaaatt gatgtatttg
     1321 atttgcattc aaataagtct agacatatta gctcgttatc aggtggagaa actttccaat
     1381 cgtcgcttgc attagcttta gggttaagcg aaattgtaca gcagcaatca ggaggtattt
     1441 cactagaatc aatatttatt gatgaaggat tcggtacatt agatcaagaa acgcttgaaa
     1501 cagcgttaga cactttatta aatcttaaat caactggtag aatggttggg attatttcac
     1561 atgtgagcga attgaaaaat agaatacctt tagttttaga agtgaaatca gatcaatatc
     1621 agagttcaac aagattcaaa agaaattaaa acataaaaaa tagagatggt actttggaat
     1681 gtatagaaca accttagccc atctcttttt atttaaaaat tattttttct cacgcaataa
     1741 atctctgatt tcagttaata acacaacatt ttcttccaca actgcttctt cttcggcttc
     1801 ttctttcttc attaatgtat ttgcaatctt aacaaagatg aataaagcaa acgcgatgat
     1861 aataaagtcg ataacagatt ggataaataa accgtattta ataccccaga atgaccattc
     1921 tttagcaaaa tcaactgatc cgaaaatttt accaattaat ggcatgatga tattttctac
     1981 taatgaagat ataatcttgt tgaaagctgc acccatcaca acagcaattg ctaaatctaa
     2041 gacgttacct tttaaggcga actctttgaa ttcttttaac a
//
LOCUS       DAOBBH010000263         2072 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.263, whole genome shotgun sequence.
ACCESSION   DAOBBH010000263 DAOBBH010000000
VERSION     DAOBBH010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.263"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(176..1084)
                     /locus_tag="P6L01_002526"
     CDS             complement(176..1084)
                     /locus_tag="P6L01_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002487080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDP3031562.1"
                     /translation="MTEQFTMIILLIALGYFLKRINFIKATDSQVIATLVLNVTLPSL
                     VIVNLNSAELKLSFSILPILMIIYGIVAKMIAVWFFRKYDNHMRGSVGMMTGAMNIGL
                     FAYPLIEVIWPKTGLIYFGMADIGGAFVMFGITYFVGSYFSEGSDQFDFKYLGKKLLQ
                     SVPLVTYIVMFILNINHIHLPHVAIDFFSILSHANMPLSMILLGVMLNFTLERKYIPA
                     TIKYLCLHYGLALVAGLLVHVLLPVSDDMIKTTLLVTWMFPVGVAIISYGIQFKYRTL
                     PFIGMTTNLTIIISIIILYVYQAVFV"
     gene            1217..1381
                     /locus_tag="P6L01_002527"
     CDS             1217..1381
                     /locus_tag="P6L01_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031563.1"
                     /translation="MSLENQLAELKYDYVRLQGDLEKRESLNLDTSALVRQLKDIENE
                     IRNVRAQMQD"
     gene            1626..1943
                     /gene="mspA"
                     /locus_tag="P6L01_002528"
     CDS             1626..1943
                     /gene="mspA"
                     /locus_tag="P6L01_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane stabilizing protein MspA"
                     /protein_id="HDP3031564.1"
                     /translation="MQFYLILLAILYLIVSFISIFKMEVVFTRILRIIMGVLLLFVLA
                     LTTMSFPKENWWVFIVLLLLVGNVEVTGFKMLKKDLKGVNILNLMSLFIFVIYFILTI
                     VLF"
BASE COUNT          731 a          355 c          292 g          694 t
ORIGIN      
        1 aactactgcc aatataactt cgtagagcat agaatattga tttatgtccc ggcctgttga
       61 acaactatgt cataaataaa agcccccttt tcacaatatg catttacatg ttgtggtaaa
      121 gggggctctc attttctacg aatactaaaa tggattttat tttcaaatgt gtaaactaga
      181 caaacactgc ctgatacacg tacaaaataa tgatactaat aatgattgtc aaattggtcg
      241 tcatacctat aaatggcagt gttcgatatt taaactgaat accataagaa ataattgcga
      301 caccaaccgg gaacatccaa gtgaccaaca atgtcgtctt aatcatatca tctgataccg
      361 gtaacaacac atgtactaac aatcccgcaa ctaatgctaa tccataatgc aaacataaat
      421 atttaatagt agcaggtata tactttcttt ccagagtaaa attcaacata acacctagca
      481 aaatcattga taacggcata tttgcatggg aaagtatgct aaagaaatcg attgccacat
      541 gtggtaaatg gatgtgattt atattcaata taaacattac aatgtatgta acgagtggca
      601 ctgactgtaa taatttctta cctaaatatt taaaatcgaa ttgatcacta ccttcactaa
      661 agtagctacc tacaaagtaa gtaattccaa acatcacaaa ggcaccacct atatcagcca
      721 taccaaaata aataagcccc gttttaggcc atatcacttc aattagtgga tatgcaaaca
      781 atccaatatt catagcacca gtcatcatac ctactgatcc acgcatgtga ttatcatatt
      841 ttctaaaaaa ccagacagca atcattttcg ccacaatacc atatataatc attaaaattg
      901 gtaaaatgga gaatgacaat tttaattctg cactgtttaa attcacaata actaaagatg
      961 ggagtgtgac attaagaact aatgtagcaa tgacttgact atctgttgcc tttataaaat
     1021 taatgcgctt caaaaagtaa ccaagcgcaa ttaataaaat aatcatagta aattgttctg
     1081 tcactgttat cccttctttc aataatcttc ataatttata actttaacat actccacaga
     1141 tattttagaa gtctactgtt tcatgctata atctacatta aatgcactta attatatttc
     1201 aaaggagtgt tatagtatgt ctttagaaaa ccaactagcc gaacttaaat atgattatgt
     1261 tcgtcttcaa ggtgacttag aaaaacggga atctttgaat ttagatactt ccgcacttgt
     1321 tcgtcaactt aaagatattg aaaatgaaat tagaaacgtt cgcgctcaaa tgcaagatta
     1381 ataatctatc attcaagcaa taaatgcttt tcgttacata aatttgacta gcattgctct
     1441 gaatacgtta tattgatgaa ttgctttatt tttcgctcaa ttacatctag aatcacaaga
     1501 tgttgtcgtg ttatgattta gtgtttcatt aacaacatac acgcatatct atcccaacac
     1561 tgctatttat gttttctatg ctgctgtact acatgaaccc tttgaaacgg agaggaagtt
     1621 atcatatgca attttatctg attttactag caatacttta tctaattgtt agttttatta
     1681 gtatttttaa aatggaagtt gtatttactc gcattttgag aattattatg ggtgtgttgt
     1741 tattgttcgt cttagcatta acgacgatga gttttccaaa agagaattgg tgggtattta
     1801 tcgtcttact actcttagtc ggcaatgtcg aagtgacagg atttaaaatg cttaaaaaag
     1861 atctaaaagg tgtaaacatc ttgaatttaa tgtcattatt tatctttgtc atatatttca
     1921 tcttaaccat cgtattattc taaataataa acaaaacatg caataacata attgcaattt
     1981 tattttatta tcgtacatgt tgaatgccag tattgtttta ttaacacatc gattaaatcg
     2041 cacgtgaaat aaggtctaaa atttcaaatt tt
//
LOCUS       DAOBBH010000264         2057 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.265, whole genome shotgun sequence.
ACCESSION   DAOBBH010000264 DAOBBH010000000
VERSION     DAOBBH010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.265"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            490..2010
                     /locus_tag="P6L01_002529"
     CDS             490..2010
                     /locus_tag="P6L01_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDP3031565.1"
                     /translation="MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILG
                     QAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVT
                     VFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGF
                     IDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEF
                     YTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQ
                     AKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRA
                     GKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSK
                     ESESRLKRISTELLNEYNDVDLVAALLQELVEANDEVEVQLTFEKPLSRKGRNGKPSG
                     SRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK"
BASE COUNT          757 a          290 c          393 g          617 t
ORIGIN      
        1 aaaaatgatt tgaattaata ctaaaagatt acaaagaaga ggtggttttg tgtgtaaata
       61 caaaattgcc tttttctttt tatgttaaat ctataaattt gaaactaaat caaggttaat
      121 tctatgtaca cactttatat aggaagtagt ttgaatgttt atataatgtt ttacaaaaag
      181 atgtagtatt ataatgtcta atttcacatg tgtttcagta aaatttgttg tggaatgtta
      241 acgatatacg tattttataa aaaatttttt ataatgatta ttcgaatgat gcgtaacgct
      301 tacatcttat ctaatgctag ctttttgaca aaaatatgac aatcaattaa tgtgattcta
      361 ataaatattc gcaaattgct ttattgcgat taaatttttt tggtggtact atatagaagt
      421 tgatgaaata ttaatgaact tatatgcaaa agtatattga gaaataaaca ggtaaaaagg
      481 agaattattt tgcaaaattt taaagaacta gggatttcgg ataatacggt tcagtcactt
      541 gaatcaatgg gatttaaaga gccgacacct atccaaaaag acagtatccc ttatgcgtta
      601 caaggaattg atatccttgg gcaagctcaa accggtacag gtaaaacagg agcattcggt
      661 attcctttaa ttgagaaagt agtagggaaa caaggggttc aatcgttgat tttagcacct
      721 acaagagaat tggcaatgca ggtagctgaa caattaagag aatttagccg tggacaaggt
      781 gtccaagttg ttactgtatt cggtggtatg cctatcgaac gccaaattaa agccttgaaa
      841 aaaggcccac aaatcgtagt cggaacacct gggcgtgtta tcgaccattt aaatcgtcgc
      901 acattaaaaa cggacggaat tcatactttg attttagatg aagctgatga aatgatgaat
      961 atgggattca tcgatgatat gagatttatt atggataaaa ttccagcagt acaacgtcaa
     1021 acaatgttgt tctcagctac aatgcctaaa gcaatccaag ctttagtaca acaatttatg
     1081 aaatcaccaa aaatcattaa gacaatgaat aatgaaatgt ctgatccaca aatcgaagaa
     1141 ttctatacaa ttgttaaaga attagagaaa tttgatacat ttacaaattt cctagatgtt
     1201 catcaacctg aattagcaat cgtattcgga cgtacaaaac gtcgtgttga tgaattaaca
     1261 agtgctttga tttctaaagg atataaagct gaaggcttac atggtgatat tacacaagcg
     1321 aaacgtttag aagtattaaa gaaatttaaa aatgaccaaa ttaatatttt agtcgctact
     1381 gatgtagcag caagaggact agatatttct ggtgtgagtc atgtttataa ctttgatata
     1441 cctcaagata ctgaaagcta tacacaccgt attggtcgta cgggtcgtgc tggtaaagaa
     1501 ggtatcgctg taacgtttgt taatccaatc gaaatggatt atatcagaca aattgaagat
     1561 gcaaacggta gaaaaatgag tgcacttcgt ccacctcatc gtaaagaagt acttcaagca
     1621 cgtgaagatg acatcaaaga aaaagttgaa aactggatgt ctaaagagtc agaatcacgc
     1681 ttgaaacgca tttctacaga gttgttaaat gaatataacg atgttgattt agttgctgca
     1741 cttttacaag agttagtaga agcgaacgat gaagttgaag ttcaattaac ttttgaaaaa
     1801 ccattatctc gcaaaggccg taatggtaaa ccaagcggtt ctcgtaacag aaatagtaag
     1861 cgtggtaatc ctaaatttga cagtaagagt aaacgttcaa aaggatactc aagtaagaag
     1921 aaaagtacaa aaaaattcga ccgtaaagag aagagcagcg gtggaagcag acctatgaaa
     1981 ggtcgcacat ttgctgacca tcaaaaataa tttatagatt aagagcttaa agatgtaatg
     2041 tcttgagctc ttttttg
//
LOCUS       DAOBBH010000265         2050 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.266, whole genome shotgun sequence.
ACCESSION   DAOBBH010000265 DAOBBH010000000
VERSION     DAOBBH010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.266"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(285..1931)
                     /locus_tag="P6L01_002530"
     CDS             complement(285..1931)
                     /locus_tag="P6L01_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDP3031566.1"
                     /translation="MNSSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYVTN
                     NIKMWITEKLGWYYLILTTIIVFFCIFLIFSPIGKLKLGKPNDKPEFNTISWFAMLFS
                     AGMGIGLVFYGAAEPMAHFATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALA
                     LAYSQFRKGEPGLLSRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQIN
                     GGLHYLFNVPNNTFVQAIIIIVVTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAAL
                     IVGPTVLILNMLTSSTGSLLNTFLFNSFDTAALNPQKREWMSSWTLYYWGWWLSWSPF
                     VGVFIARVSKGRSIREFISGVLLVPAIVSFVWFSVFGVLGIETGKKHKEIFDMTPETQ
                     LFGVFNHVPFGIVLSLIALLLIASFFITSADSATFVLGMQTTFGSLNPSSMVKVVWGI
                     SQALIAFVLLLAGGGNGAEALNAIQSAAIISAFPFSFVVILMMVSFYKDANQERKFLG
                     LTLTPNKHRLQEYIKSQQEDYESDILEKRQSRRNIEKKDN"
BASE COUNT          737 a          394 c          299 g          620 t
ORIGIN      
        1 attgatctat gtcccagtct gagttaattt tctataaaag tatagttaat ttttttatac
       61 cgtcgtactt cactttagct ttgtcaaacc tctttctatt aagttgtcag aaataaacct
      121 atcttaaaat ataaaaaaat cgagaattcg tagtttaata acgaaattct cgttcttatc
      181 cttttgaata tactcaattt tccacaaaaa caaacaagta gtatatctgt tctagctact
      241 agaatgacat actacttgtt atttaaatac ttaactaaat tttattagtt atcttttttc
      301 tctatatttc tacgtgactg acgcttttca agaatgtcag attcataatc ttcttgttga
      361 ctcttgatat attcttgtaa gcgatgttta ttcggagtca atgttaaacc taggaattta
      421 cgttcctggt tcgcatcctt gtagaaactt accatcatga gtatgacgac aaaggagaat
      481 gggaatgcac ttataattgc agcactttga atcgcattta aagcttcagc gccattaccg
      541 ccaccagcta ataaaagtac aaatgctatt aaggcctgtg aaattcccca aacaactttt
      601 accatactag atggatttaa tgaaccaaat gttgtttgca ttcctaatac aaatgttgct
      661 gagtcagcag atgtaataaa gaatgatgca attaataata atgcaatcaa cgataaaact
      721 atgccaaatg gcacatgatt aaacactcca aatagctgtg tttcaggagt catatcaaaa
      781 atttctttgt gtttcttacc tgtctcgatg cctaatacac caaagacact aaaccaaaca
      841 aaactaacaa ttgctggaac tagcaagaca ccagaaatga actctctaat tgaacgtcct
      901 tttgaaactc gtgcaataaa cactccaacg aatgggctcc aacttaacca ccaaccccag
      961 taataaagtg tccatgaaga catccattca cgtttttgag gatttaaagc agctgtatca
     1021 aaactattaa acaagaatgt gtttagtaaa ctacccgtag agctagttaa catatttaaa
     1081 ataagaacag ttggtccaac aattaaagca gctaccatta aaatagtacc taaaccaatg
     1141 ttcaagttac ttaagtattg aataccttta cttaatccag accatgcact tgctataaat
     1201 aagatagtaa caacaatgat gataatcgct tgtacaaacg tattgtttgg aacattgaac
     1261 aagtaatgta aaccaccatt aatttgtaga gcacccatac ctaacgaaac ggctacccca
     1321 acgattgtcg caaatacaga taaaacgtca ataaaaatcc caataggacc ttctacttta
     1381 tcacctaaaa gaggacgtaa agttctagat aataaacctg gttcaccttt acggaattgc
     1441 gaatatgcca acgctaacgc aacaacacca taaacagccc aagcatggaa tccccaatgg
     1501 aaaaatgttg aacgtagagc ttcagtataa gcttcagtag ttttgggatc tgctgtaggt
     1561 ggcgtagcaa agtgcgccat cggttcagct gcaccataaa acaccaaacc tatccccata
     1621 ccagcactaa acaacatagc aaaccatgaa attgtattaa actcaggttt gtcatttggt
     1681 ttacctagtt taagttttcc aataggacta aaaataagga atatacagaa gaacacgata
     1741 atcgtagtaa gaataagata ataccaacct aacttttctg taatccacat tttaatatta
     1801 ttggtaacat agttgaattg ttcaggtaaa aatgcaccaa gtaatacgac tatagcaaca
     1861 acaattgcac tatagatgaa gactggtgaa tacttctttc catttggatt ctctggtgaa
     1921 gaagaattca taattaatta ctcccttcaa ttctatattt aattttatgt agtagaataa
     1981 aaagattatc taaacatttt attcaataac tcacgcacct ttataggata acaaaggtct
     2041 ttgcgataat
//
LOCUS       DAOBBH010000266         2037 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.267, whole genome shotgun sequence.
ACCESSION   DAOBBH010000266 DAOBBH010000000
VERSION     DAOBBH010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.267"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            725..2020
                     /gene="purB"
                     /locus_tag="P6L01_002531"
     CDS             725..2020
                     /gene="purB"
                     /locus_tag="P6L01_002531"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDP3031567.1"
                     /translation="MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKAD
                     VQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTAL
                     SFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
                     MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDR
                     HAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSEN
                     ITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRL
                     TVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFREL
                     IEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA"
BASE COUNT          774 a          269 c          365 g          629 t
ORIGIN      
        1 tttactaaat tgcaaacatc tcattcaata attaatatga tcgaaagttt catgaaaata
       61 tattagtcga ttttgaaaga agcgtagtca atttatgtct ttctataaaa atttactatt
      121 aaaaaaatct ctgcgtattt ttctataatc agtttcgtat ttatacgatt ctggagttca
      181 atattaatgt cttatattta aaatgccaca cctatgcaat agaatatata ttctttttgt
      241 gagtagaaaa tttgttatat tagaagcgaa taattttaaa acatattatg aaatgtaaga
      301 agaaatatta tgaaactagg aaaagtagat tgttgaaaat tccatacaat aggagaagta
      361 aagtgaataa attctggtat tactaaagct attgattgtc ctaattgtta taaaaagttg
      421 gatagtgtag ttgaggggag aaaaactaaa atgcaaaaat atattcttaa tggatcatta
      481 taaataccaa ttagattaaa gtattgcgaa catttacaat tagaaatcag tattaatctg
      541 ccatagataa ttgtattaat gtaaaaaata aaatagaatg agaatatacg gaaatttttt
      601 atacatttat aaccaattct gttaaactgt gaagagtaga gttaaaaacg aactttttgt
      661 ttatttttat taaaaatgtt ctgtttttga aaattcgagt atacatacga taggagttaa
      721 aaaaatgatt gaacgctatt ctagagaaga aatgtctaat atttggactg atcaaaatcg
      781 ctatgaagca tggttagaag tggaaatttt agcatgtgaa gcatggagtg aattaggaca
      841 tataccgaaa gctgacgtac aaaaaattcg tcaaaacgca aaggtaaatg tcgaacgtgc
      901 acaagaaatt gaacaagaaa cgcgccatga tgttgtagcc tttactagac aagtttctga
      961 aacgctaggt gaagaacgta agtgggtaca ttatggttta acttctactg atgttgtaga
     1021 tacagcttta agtttcgtta ttaaacaagc aaatgacatt attgaaaaag atttagaaag
     1081 atttatcgac gttttagctg aaaaagcaaa aaattataaa tatacattga tgatgggacg
     1141 tacacatgga gtgcatgcag aaccaactac atttggtgtc aaaatggcat tgtggtacac
     1201 ggaaatgcaa cgtaacttac aacgtttcaa acaagtaaga gaagaaattg aagtagggaa
     1261 aatgagtggt gcagtaggta cttttgctaa cattcctcct gaaatagaaa gttatgtgtg
     1321 taaacattta ggtattggca cagcgcctgt atcaacgcaa acattgcaac gcgatcgaca
     1381 tgcatactat attgcgacat tagcattaat cgcgacgtca ctagaaaaat ttgccgttga
     1441 aattcgtaac cttcaaaaaa ccgaaacaag agaagttgaa gaagcatttg caaaaggcca
     1501 aaaaggttca tctgcaatgc ctcataaacg aaatccaatt ggttctgaaa atatcacagg
     1561 tatttcaaga gtcattcgtg gttatattac tacagcttat gagaatgtgc cattatggca
     1621 tgaaagagat atttctcatt cttctgctga gcgtattatg ttaccagatg ttacaatagc
     1681 actagattat gcattgaatc gtttcacaaa tattgtggac cgtttaactg tatttgaaga
     1741 taatatgcgt aataacatag ataaaacatt tggtctgatt ttctcacaac gtgtcttatt
     1801 agcattaatt aataaaggta tggttcgtga agaagcatac gacaaagtac aaccaaaagc
     1861 gatgatatct tgggaaacga aaacaccttt ccgtgaatta atcgaacaag atgagtctat
     1921 tacaagtgtt ttaacaaaag aagaattaga tgaatgtttc gatcctaaac atcatttgaa
     1981 tcaagttgac actattttcg aacgcgctgg attagcataa aattaaataa agtaacg
//
LOCUS       DAOBBH010000267         2025 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.268, whole genome shotgun sequence.
ACCESSION   DAOBBH010000267 DAOBBH010000000
VERSION     DAOBBH010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.268"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..1447)
                     /locus_tag="P6L01_002532"
     CDS             complement(<1..1447)
                     /locus_tag="P6L01_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDP3031568.1"
                     /translation="MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVST
                     SIFTLPGIVAAEHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFG
                     EFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDI
                     IAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEH
                     KVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSVAIV
                     LFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMML
                     AGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAG
                     TLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAITFVLVLLVFWGLGFEAKLY
                     TLIWFIVGIILYLSYGLRHSKKNDVAEYHPPK"
BASE COUNT          734 a          363 c          330 g          598 t
ORIGIN      
        1 attttggtgg gtggtattca gctacatcat ttttcttaga atgtctaagt ccataactta
       61 aatatagaat gataccaaca ataaaccaaa ttaaagtgta taatttcgct tcgaatccta
      121 atccccagaa tactagcaat actaaaacaa atgtaattgc tggtaacaca ggatataaag
      181 gtaatttaaa tgcaggaatt ggtagatctt taccttcacg ctttctcaaa cgatacattg
      241 ctaatgaaac gaacataaat gcaacaagtg tacctgctga aattaattgt gctaaaaatg
      301 cgaatgggaa catagaacca attaaaacac caataatagt aagtataact agtgcgcgat
      361 taggtaaatg tttgtcgttt aaatggctta accatgaagg taataagccg tcacgtccaa
      421 atgaataaag taaacgtgag cctgctaaca tcataccaat taatgctgta aacataccga
      481 taacagagat agcttgaaca atagctgcta caacaccatg accactttga cgtaaagccc
      541 aaccaacagg ttcagcattg tttgcgtatt gtgagtaatg gaacatacca actaacacaa
      601 gtgctacagc aataaataat acgatagcaa cacttaaaga accaagaata cctctaggca
      661 ttgtcttttg agggtcaagt gcttctgctg agtttgctgc gatagaatcg aaaccgatat
      721 acgctaagaa aatcattgaa acaccagcat atatgccttg ccatccacca aagtcacctg
      781 tagcagttac tttgtgttct ggaataaatg gcacatagtt actaacattt attgctgtta
      841 aacctacgat gacaaataaa ataatagcta atacttttaa aataactaaa atgttttcca
      901 tacgtgctgc ttctgacata ccacgtgata gtaataatgc agttaataaa ataacgatag
      961 cagcaataat atcgataaaa ccgccattcg taccaaatgg atttgataat gctgcaggta
     1021 attcgatgcc aattggtttc acaagtccgc gtaaattcgc tgagaatcct gatgcaacaa
     1081 aggctacggc gataaaatat tcagctaata gagcccaacc ggcaacccat ccaaaaaatt
     1141 caccaaataa tacattgacc caagaatagg ctgaacctgc aaatggcata gcggcagcca
     1201 tttctgcata agtaaatgca actaaaccag caacaatagc agcgagtaag aatgataacg
     1261 caacggccgg tcctgcatgt tctgcagcaa caatgccagg tagcgtaaag atagatgtcg
     1321 atacaattgt tcctacacct aaagctaaga aatcacgcac ccgaagtgta cgctttaaat
     1381 gaccatcttt attttgatag atagccggat cctcttttcg tgctatttta ttgaaaaaac
     1441 ttcccataaa ctttcctccc aaacattcat aaacaattct atacggtgtt ttttaatatg
     1501 ttatatcata gcacaaataa tcaatatttt gtctaaaaat tctgaaaaat cacaacttta
     1561 tgttacgtat taatgacttg tcttgataac atccatagat tttttaaatg ataaaactga
     1621 ttataacaga tattaaatga ataagtacta tttttgctaa ttttctaaca attttgcaca
     1681 ttatattttt aaaatcaatt tcatgtttat gtctgattgg ctagtttgta tgaaatgtaa
     1741 ttctttgact ataaaatgat gcatcaaata tgatgagggt aaggcggaaa ataggaggtt
     1801 atatagtaag tcatttaagt cgaatagcca tcctttttaa aatgaaaaaa atagaaagct
     1861 caatagtttg ttaaaggctt taaatagcgt cattacgcaa ttttagaatg ctaaaaattg
     1921 tcacaaattt caaattacgt gctaaagctt gagatatcaa tatttattgg cgatagagtg
     1981 taatttgact ccgcttacat ctcagtttta tgtttgtaat ttggt
//
LOCUS       DAOBBH010000268         2025 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.269, whole genome shotgun sequence.
ACCESSION   DAOBBH010000268 DAOBBH010000000
VERSION     DAOBBH010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.269"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(473..1666)
                     /gene="fmtA"
                     /locus_tag="P6L01_002533"
     CDS             complement(473..1666)
                     /gene="fmtA"
                     /locus_tag="P6L01_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala esterase FmtA"
                     /protein_id="HDP3031569.1"
                     /translation="MKFNKVKLVIHACVLLFIIISIALIFHRLQTKTHSIDPIHKETK
                     LSDNEKYLVDRNKEKVAPSKLKEVYNSKDPKYKKIDKYLQSSLFNGSVAIYENGKLKM
                     SKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYLPWFK
                     TSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVKAIQKRGIDPKKYKKHMYNDGNYLV
                     LAKVIEEVTGKSYAENYYTKIGDPLKLQHTAFYDEQPFKKYLAKGYAYNSTGLSFLRP
                     NILDQYYGAGNLYMTPTDMGKLITQIQQYKLFSPKITNPLLHEFGTKQYPDEYRYGFY
                     AKPTLNRLNGGFFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILKQNKPYNTK
                     GVIVQ"
BASE COUNT          674 a          325 c          223 g          803 t
ORIGIN      
        1 tcaattttta aatggttaag tccccttcta tatcttataa gacaatcatt ataatcaatt
       61 caattaatac attaacaacc acaactaata aatatagtaa cttcaaaatc catatttatg
      121 tctaaagata atctcaatgt tgttcacgtc aataaaatta tccctaggtt tttaaaaatt
      181 gtacatgttt aaacaatcaa aagtgtacat tattaaatta tcatttccag ttagatttag
      241 aaaacattca caccacgcat ggaccaacgt attcgtcttc attcatttta gtctgatgcc
      301 aactcaccaa atgatttccc cttttatcag ttaacacata tttttaaact catccttctt
      361 caaccttata ctttaaactt tttaccacta taaaaaaaaa gatataactc gtatactggt
      421 cagataaggt cgatgcctta atgccatata catgttatat cttctatatt aattattgaa
      481 caataacacc cttcgtattg taaggtttat tttgttttaa aatatcatta taaatatgtt
      541 tgattcgaac ttcattgttt ccttttacat ttaatgcaag tacaactaca tacttatcat
      601 tatagtaaac agtaaagact tgtccaaaga atcccccgtt aagtctattc aatgttggct
      661 tagcatagaa accatatcga tattcatctg gatactgttt cgtaccaaac tcatgtaata
      721 atggattggt tattttagga ctgaataatt tatattgttg tatttgagta attaatttac
      781 ccatatctgt tggtgtcata tataaattac ctgcaccata gtattggtcc aaaatattag
      841 gtcttaagaa tgaaagtcct gtactattat aagcataacc ttttgctaga tattttttaa
      901 aaggttgttc atcataaaat gctgtgtgct gaagttttaa aggatctcct atttttgtat
      961 aataattctc agcataagat ttacctgtaa cttcttcaat aactttcgca agtactaaat
     1021 aattcccatc gttatacata tgctttttgt atttcttagg atcaatacca cgtttttgaa
     1081 tcgctttaac tgcttgatct aaatttttat aatcttttga ggatttatat ttatataatc
     1141 cactttgatg caacattaaa tctttcaatg ggataggctt agatgtttta aaccatggaa
     1201 ggtatttact tactggatca ttgatattta ttttatgttc ttcttctaac tgttttaaca
     1261 gtaaccctgt tgaaaatttt tgagctgaac ctattaaaaa catcgtattc ggtgtgtttt
     1321 taataccttt ttcaaaatct tgatatccat aacctttact cattttcaat ttgccatttt
     1381 catatatagc tactgaaccg ttaaataatg aactttgtaa atacttgtca attttcttat
     1441 atttaggatc cttgctatta tatacctctt ttagtttaga cggcgcaacc ttttccttat
     1501 tacgatccac taaatatttt tcattgtctg ataattttgt ttccttatgt attgggtcta
     1561 tagaatgtgt cttcgtctgt aatcgatgaa aaattaacgc tatagaaata atgataaata
     1621 atagtacaca cgcatgtata actagtttta ctttattaaa tttcatttga taattgcctc
     1681 acaatgtatt cattttatat atttaaatcg ttaaaaattt gaacctagat gcctaataat
     1741 acaacttaca aaaattaact tatcattcat ttatgcttta cgctacatag taataagttt
     1801 ttctaatatg ttatctatac aacaaaatta attatacctt atcactttgt ataaatcact
     1861 cgaatttata ttacgccttt aaatttttta agacatcata ctaaagtcgc attatatttt
     1921 taaaaatgct ttttgatttt aacaaaacat tcaaattaca aggaactttg acataacatt
     1981 tgtatttttt actataaagt actacaattt aaggctataa tgtat
//
LOCUS       DAOBBH010000269         2004 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.270, whole genome shotgun sequence.
ACCESSION   DAOBBH010000269 DAOBBH010000000
VERSION     DAOBBH010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.270"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            122..988
                     /gene="galU"
                     /locus_tag="P6L01_002534"
     CDS             122..988
                     /gene="galU"
                     /locus_tag="P6L01_002534"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDP3031570.1"
                     /translation="MKKIKKAIIPAAGLGTRFLPATKAMPKEMLPILDKPTIQYIVEE
                     AARAGIEDIIIVTGRHKRAIEDHFDSQKELEMVLKEKGKSELLEKVQYSTELANIFYV
                     RQKEQKGLGHAISSARQFIGNEPFAVLLGDDIVESEVPAVKQLIDVYEETGHSVIGVQ
                     EVPEADTHRYGIIDPLTKNGRQYEVKKFVEKPAQGTAPSNLAIMGRYVLTPEIFDYLK
                     TQKEGAGNEIQLTDAIERMNNDNQVYAYDFEGERYDVGEKLGFVKTTIEYALKDDSMR
                     EELTRFIKALGL"
     gene            complement(1199..1942)
                     /gene="sarU"
                     /locus_tag="P6L01_002535"
     CDS             complement(1199..1942)
                     /gene="sarU"
                     /locus_tag="P6L01_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarU"
                     /protein_id="HDP3031571.1"
                     /translation="MDYQTFEKVNKFINVEAYIFFLTQELKQQYKLSLKELLILAYFY
                     YKNEHSISLKEIIGDILYKQSDVVKNIKSLSKKGFINKSRNEADERRIFVSVTPIQRK
                     KIACVINELDKIIKGFNKERDYIKYQWAPKYSKEFFILFMNIMYSKDFLKYRFNLTFL
                     DLSILYVISSRKNEILNLKDLFESIRFMYPQIVRSVNRLNNKGMLIKERSLADERIVL
                     IKINKIQYNTIKSIFTDTSKILKPRKFFF"
BASE COUNT          729 a          263 c          332 g          680 t
ORIGIN      
        1 aattaaatca aatttcccaa gaaataatta catactcaat gacactatga aggcgttcta
       61 attagtgtta aaatgacgtt gatacataga tttaatactt aggaaaagga gcacattaac
      121 tttgaaaaaa ataaaaaagg caatcattcc cgctgctggt ttagggacta gatttttacc
      181 agcaactaaa gcgatgccaa aggaaatgct tcctatctta gataaaccca caatacaata
      241 tatcgttgaa gaagctgcaa gagctggaat tgaagatatt attatagtga caggtcgcca
      301 caaacgcgcg attgaagatc attttgatag tcaaaaagaa ttagaaatgg tgttaaaaga
      361 aaaaggtaaa tctgaattac tagaaaaagt tcaatactcg acagaacttg cgaatatttt
      421 ttatgtaaga cagaaagaac aaaaaggttt agggcatgcg attagttctg cgcgtcaatt
      481 tatcggcaat gaaccatttg cggtattatt aggtgacgat attgttgaat ctgaagtgcc
      541 agcagtgaaa cagttgattg acgtttatga agaaacgggg cactcagtta taggtgtcca
      601 agaagttccg gaagcagata ctcatcgtta tggaattatt gatccattaa ctaaaaatgg
      661 acgtcagtat gaagtgaaaa agtttgttga aaagccagca caaggtacgg caccatcaaa
      721 cctagctatt atgggccgat atgtattaac gcctgaaatt ttcgattatt taaaaacgca
      781 aaaagaaggc gcaggtaacg aaattcagtt aacagatgcg attgagcgta tgaacaatga
      841 taatcaagtg tatgcatatg acttcgaagg cgaacgttat gacgttggag aaaaattagg
      901 atttgtcaaa acaacaatag aatatgcgtt gaaagatgac agtatgcgag aagaattaac
      961 acgatttatt aaagcattag gtttataata ttaaaagcat gatactcaat aaaattggaa
     1021 cacgaaatgg tgaactaaat tgaatgagtg tcatgcttaa tttatgtgtt gtgatatata
     1081 gtagggtatt gaaataatat aaaaacaaaa gaaagttaat tatgtatttt tatttatact
     1141 aaattgagaa gtattatgat tgcttattta ttttatcaag aggtaaatct gtttaaattt
     1201 aaaagaaaaa ttttcttggt ttgagaatct tggaagtatc tgtgaatatg cttttaatag
     1261 tgttatattg tattttattt attttgatta acacaatcct ttcatctgca agggatcgtt
     1321 ctttgattag cataccttta ttatttaatc tattaactga cctaacaatt tgaggataca
     1381 taaatctaat actttcaaac aaatctttta aatttagtat ctcatttttt cgagatgata
     1441 ttacatataa gatagataaa tcaagaaatg ttaaattaaa tcgatatttt aaaaaatctt
     1501 ttgagtacat aatgttcata aaaagtataa aaaattcttt gctatatttt ggagcccatt
     1561 gatattttat gtagtctctt tccttattaa atcctttaat tattttatct aactcattaa
     1621 taacacaagc aatcttttta cgttgtattg gagtaactga aacaaaaata cggcgttcat
     1681 ctgcttcgtt tctagactta tttataaatc cttttttaga tagtgactta atgttcttta
     1741 caacatcaga ttgtttgtaa agtatgtcac caatgatttc ttttagtgaa atactgtgtt
     1801 catttttgta ataaaaatat gctaatatca acaattcttt taatgataat ttatattgtt
     1861 gctttaactc ttgagtgaga aaaaatatgt acgcttctac atttatgaat ttattgacct
     1921 tttcgaaagt ttgatagtcc accttacatt ctcctactat tttctgatgt cccaatactt
     1981 tatattattt aaagtaatat aaaa
//
LOCUS       DAOBBH010000270         1992 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.271, whole genome shotgun sequence.
ACCESSION   DAOBBH010000270 DAOBBH010000000
VERSION     DAOBBH010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.271"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            103..771
                     /gene="queC"
                     /locus_tag="P6L01_002536"
     CDS             103..771
                     /gene="queC"
                     /locus_tag="P6L01_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDP3031572.1"
                     /translation="MESVLNNEKAIVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQR
                     HDTEIEVAKQIAQDQGMKHHVLDMSLLSQLTPNALTQHDMEITNNEDGIPNTFVPARN
                     LLFLSFAGALAYQIGAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLAMDKDFVIHT
                     PLMWLNKAETWKLSDELEVLDYIRTKTLTCYNGIIGDGCGECPACHLRQRGLNQYLES
                     KGAL"
     gene            773..1192
                     /gene="queD"
                     /locus_tag="P6L01_002537"
     CDS             773..1192
                     /gene="queD"
                     /locus_tag="P6L01_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDP3031573.1"
                     /translation="MLQQIYPSTTHPYQFELNKDFNFSAAHHIPCEEAGICQNVHGHT
                     YFVNLTIVGDKLDDTGFLVNFSHLKKMIHGKFDHQLLNNLPAFKNKIPSTEIVAETIY
                     QIVKENLASLEHQPKCIQVFVRETPTSYVVFRPKEQV"
     gene            1196..1909
                     /gene="queE"
                     /locus_tag="P6L01_002538"
     CDS             1196..1909
                     /gene="queE"
                     /locus_tag="P6L01_002538"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDP3031574.1"
                     /translation="MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAF
                     TWDGSAKGDIKLMTAEEIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGI
                     FSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVF
                     DDKDYDFAKMIHHRYPDIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMN
                     HVYVLPQLHTLLWSNKKGV"
BASE COUNT          706 a          318 c          349 g          619 t
ORIGIN      
        1 cttttatgga gataggcttt ttttatttta taaattaaaa acgggcttat caatgccatt
       61 aacctcttta ttaatattat taatgatagg agcaattaca aaatggaaag tgtattaaat
      121 aatgaaaaag ccatcgttgt ctttagtggt ggtcaagaca gtacaacatg tctcttttat
      181 gcaaaaaaac atttcaaaga agttgaactc gtaacgttta attatggcca aagacatgat
      241 actgaaattg aagtcgcaaa acaaattgca caagatcaag gaatgaaaca tcacgtttta
      301 gatatgtcat tattatcaca acttactcca aacgcattaa cacaacatga tatggaaatt
      361 actaataatg aagatggtat acctaataca tttgttccag ctagaaattt acttttcttg
      421 tcgtttgcag gcgctctagc ttatcaaatt ggggctaagc atattattac aggcgtatgt
      481 gaaacagact tttcaggcta cccagactgt cgcgatagtt ttataaaatc aatgaacgta
      541 acattaagcc tagctatgga caaagatttt gtcattcata ctcctttaat gtggttaaac
      601 aaagcagaaa cgtggaaatt aagtgatgaa ctcgaagttt tagattatat tcgtacaaaa
      661 acattaacat gctataacgg tatcattggg gatggctgtg gtgaatgtcc agcttgtcat
      721 ttacgtcaac gtggactaaa tcaatatctt gaaagtaaag gagcgcttta aaatgttaca
      781 acaaatctat cctagtacaa cgcatccata tcaattcgaa ttaaataaag attttaattt
      841 ttcggctgca catcacattc cttgtgaaga agcaggtatt tgtcaaaatg tccatggtca
      901 tacttacttt gttaatttaa caattgtcgg tgataaacta gatgacactg gcttcttagt
      961 gaactttagc catttgaaaa agatgataca cggtaaattt gaccatcaac tgttaaataa
     1021 cttacctgct tttaaaaaca aaatcccttc aactgaaatc gtagcggaaa caatttatca
     1081 aattgttaaa gaaaatttgg catcgctcga acaccaacca aaatgtattc aagtatttgt
     1141 aagagaaaca ccaacaagtt atgttgtatt tagaccaaag gaacaggtgt aactaatggc
     1201 taaaattcct gtactcgaaa tattcggtcc aaccattcag ggtgaaggtc gagtaatcgg
     1261 tagaaaaacg atgtttgtga gaacagctgg atgtgattat cgctgtagct ggtgtgattc
     1321 tgcatttact tgggatggta gtgctaaagg cgatataaaa ctcatgaccg ctgaagaaat
     1381 ttatgatgaa ttaaaacgaa ttggtggcga tttatttaat cacgttacaa tttctggtgg
     1441 taacccagca ttaattaaag gtatccaaga gttagttgac ttatttcaag ataaaggtat
     1501 tttcagcgca ctggagacac aaggcagtaa attccaacct tggatgacac aaattgatga
     1561 tttaacgatt agtccaaaac caccaagttc aactatgaca cctgatttaa aaaagttaga
     1621 tgaagtgatt acacaatgcg taccttcctc attaaactta aaagtagttg tattcgacga
     1681 taaagattat gattttgcca aaatgataca ccaccgttat ccagatattc ccttctattt
     1741 acaagttggt aacccatatt tatcagacag cgtagataat cataccgaaa agttgttaga
     1801 acgctacgag cagttggtgg atttagttat gcaaagtaat gatatgaatc acgtttatgt
     1861 gttaccacaa cttcatacat tactttggag taataaaaaa ggtgtataaa ggataactta
     1921 ttttactttt tacaagtcat atctaatgaa taacaatata ttaattgata taatagttga
     1981 ttgaagtata ac
//
LOCUS       DAOBBH010000271         1941 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.273, whole genome shotgun sequence.
ACCESSION   DAOBBH010000271 DAOBBH010000000
VERSION     DAOBBH010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.273"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..996
                     /gene="argF"
                     /locus_tag="P6L01_002539"
     CDS             <1..996
                     /gene="argF"
                     /locus_tag="P6L01_002539"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDP3031575.1"
                     /translation="NLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLKNK
                     NIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYDGI
                     EYRGFSQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTYVG
                     DGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIAAENGGNILITDDIDQ
                     GVKGSDVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEMMDKTGNPNVIFEHCLPSFH
                     NADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF"
     gene            1019..>1941
                     /gene="arcC"
                     /locus_tag="P6L01_002540"
     CDS             1019..>1941
                     /gene="arcC"
                     /locus_tag="P6L01_002540"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDP3031576.1"
                     /translation="MAKIVVALGGNALGKSPQEQLELVKNTAKSLVGLITKGHEIVIS
                     HGNGPQVGSINLGLNYAAEHNQGPAFPFAECGAMSQAYIGYQLQESLQNELHSIGMDK
                     QVVTLVTQVEVDENDPAFNNPSKPIGLFYNKEEAEQIQKEKGFIFVEDAGRGYRRVVP
                     SPQPISIIELESIKTLIKNDTLVIAAGGGGIPVIREQHDGFKGIDAVIDKDKTSALLG
                     ANIQCDQLIILTAIDYVYINFNTENQQPLKTTNVDELKRYIDENQFAKGSMLPKIEAA
                     ISFIENNPKGSVLITSLNELDAALEGKVGTV"
BASE COUNT          722 a          269 c          386 g          564 t
ORIGIN      
        1 aatttacgaa acagaagttt tttaacttta ttagactttt cacgacaaga ggtagaattc
       61 ttattaacac tctccgagga tttaaaacgt gctaaatata ttggcactga aaagcctatg
      121 ttaaaaaata aaaatattgc actgttattt gaaaaagatt ctacaagaac gcgatgtgca
      181 tttgaagttg cagcgcatga tcaaggtgca aatgtaactt atttaggccc aactggatca
      241 caaatgggta aaaaagaaac aactaaagat actgcacgtg tgcttggtgg aatgtatgat
      301 ggcattgaat accgtggttt ttcacaaaga acagtagaaa ctttagctga atattcaggc
      361 gtaccagtgt ggaatggttt aactgatgaa gatcatccta ctcaagttct tgctgatttc
      421 ttaacagcaa aagaagtctt aaaaaaagat tatgcagata ttaactttac atatgttgga
      481 gatggtcgta ataacgttgc aaatgcatta atgcaaggtg ctgccattat gggtatgaac
      541 ttccatttag tttgtccaaa agaattaaat ccaacagatg aattattaaa tcgctgtaaa
      601 aatattgccg ctgaaaatgg tggcaacata ttaatcacag atgatattga ccaaggtgta
      661 aaaggttcgg atgtaattta cactgatgtt tgggtatcaa tgggtgaacc tgatgaagta
      721 tggaaagaac gacttgaatt attgaaacca tatcaagtaa ataaagaaat gatggataaa
      781 actggtaatc caaatgttat ttttgagcat tgcttaccat ctttccataa tgctgatacg
      841 aaaattggtc aacaaatttt tgaaaaatat ggtattcgag aaatggaagt tacagatgaa
      901 gtattcgaaa gtaaagcttc agttgtattc caagaagctg agaacagaat gcatacaatc
      961 aaagcagtca tggttgctac attgggtgaa ttttaaatga tataaggaag tgaatatgat
     1021 ggcgaaaata gtagtagcat taggtggtaa tgctttagga aaatcacctc aagaacaact
     1081 cgagcttgtt aaaaatactg cgaaatcatt agtaggatta ataacaaaag gacatgagat
     1141 tgttattagt catggtaatg gaccacaggt tggaagcatt aatttgggac ttaactatgc
     1201 tgcagaacat aaccaaggtc cggcatttcc atttgctgaa tgtggcgcaa tgagtcaagc
     1261 ttacatcggc tatcaattac aagaaagctt acaaaatgaa ttgcattcta ttggaatgga
     1321 taaacaagtg gtaacactag tgacacaagt tgaagttgat gaaaatgatc cggcatttaa
     1381 caatccttca aaaccaattg ggttatttta caacaaagaa gaagctgaac aaattcaaaa
     1441 agaaaaagga tttatatttg ttgaagatgc tggaagagga tatagacgcg ttgttccttc
     1501 accacaaccc atctctatta ttgaattaga gagtattaaa acacttatta aaaatgatac
     1561 actcgttatt gctgctggtg gtggaggtat accagtaatt agagagcaac atgatggttt
     1621 taaaggtatt gatgcagtta tagacaaaga taaaacaagt gcgttgttgg gtgctaatat
     1681 tcaatgcgat caattgatta ttttaacagc aattgattat gtatatatta attttaacac
     1741 tgaaaaccaa cagcctttga aaacaacaaa tgttgatgaa ttaaaacgat atatagacga
     1801 aaatcaattt gcaaaaggaa gtatgttacc aaaaattgaa gcagccatat catttattga
     1861 aaacaatcca aaaggaagtg tgcttataac atcattaaat gaattagatg ctgccttaga
     1921 gggtaaagta ggtactgtga t
//
LOCUS       DAOBBH010000272         1934 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.274, whole genome shotgun sequence.
ACCESSION   DAOBBH010000272 DAOBBH010000000
VERSION     DAOBBH010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.274"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..860
                     /locus_tag="P6L01_002541"
     CDS             <1..860
                     /locus_tag="P6L01_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDP3031577.1"
                     /translation="TLLTHYISELLKNDEKIKIIMNEFGTFDIDSNSISNEIEVHSLI
                     NGCVCCDLKQELVYELKAIALKGDVNHVIIEATGIAHPLELLVACQDPQIVNFFEKPI
                     IYGVLDATRFLERHQYTENTVSLMEDQLKLSDMIIINKIDLITDDSLEKIDKQLGMIC
                     ASIPTYKTTYGKVSLEELDLTVKDREISSHHHHHHGIKSMTYTFTGPIDRQLFYQFIM
                     KLPESVLRLKGYVSFRNQPNAIYEFQYAYGLPDYGIIGMQLPLTIVIIGETLDTNHIR
                     NQLDMLQFT"
     gene            963..1871
                     /locus_tag="P6L01_002542"
     CDS             963..1871
                     /locus_tag="P6L01_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HDP3031578.1"
                     /translation="MEQIMINHYVHFSRLVQGFWRANEWKMTAKELNYFINELVERGI
                     TTMDHADIYGDYQCESLFGNALDLSPELRNKIQIVTKCGIILPSKQFDFTNGHRYDLS
                     SKHIVKSVEQSLINLNVDYLDSLLIHRPSPLMDPEQVADALTKLVKQGKLKSFGVSNF
                     NHSQYQLLNQYIMKERLHISINQLELSPYHVDSLQDGTMDSMYQNHVQIMAWSPFAGG
                     KIFDKEDIKAQRIMKVVQSIADKYGVSDTAVMIAWLVKIPHSIMPILGTSQLKRIDQA
                     IEGLQLNLDDQSWFDIYTAIIGQDIP"
BASE COUNT          671 a          270 c          356 g          637 t
ORIGIN      
        1 ccacgttact gacacattat attagtgaat tattaaaaaa tgatgagaaa attaaaatca
       61 tcatgaatga attcggtact tttgatattg atagcaatag tatttcaaat gaaattgaag
      121 tccattcatt gattaatggt tgtgtttgtt gcgatcttaa acaagaactt gtctatgaac
      181 taaaagccat tgctttaaaa ggggacgtta atcatgtcat catagaagcg acaggcattg
      241 cgcatccttt ggaattacta gttgcatgtc aagatccgca aatcgttaat ttctttgaaa
      301 agccgattat ttatggtgta ttagatgcga ctcgattttt agaacgtcat caatataccg
      361 aaaatacagt ttcgctgatg gaagatcagt tgaaactaag tgacatgatt attattaata
      421 aaattgatct tataactgat gacagtcttg agaaaattga taagcaatta ggtatgattt
      481 gtgcaagtat tccaacttat aaaacaacct atggaaaagt ttcgttggaa gaattggact
      541 taactgttaa agacagagag atatcgtctc atcatcacca tcatcatggg attaaaagta
      601 tgacttacac gtttacaggt ccgattgatc gtcaattgtt ttatcaattt ataatgaaat
      661 taccggaatc tgttctacgt ttgaaaggtt atgtgtcatt tagaaatcaa ccaaatgcaa
      721 tttatgaatt tcaatatgca tatggtttac cagactatgg aataattggc atgcaattac
      781 cattaacgat tgttattatt ggtgaaactt tagatacaaa tcacatacgt aatcaattgg
      841 atatgctaca atttacgtaa atcagaaaat gtgttgtttc aatttaacgg aaatagcaca
      901 ttaatttctt aatactttta ttgtgaatat tttgtgacat tgagggatgg agtggatgag
      961 atatggaaca aataatgatt aatcactatg ttcatttttc taggcttgta caaggttttt
     1021 ggcgtgcaaa tgaatggaag atgactgcga aagagttaaa ttattttata aatgaattag
     1081 ttgaacgtgg aattacaacg atggatcatg ctgatattta tggggattat caatgtgaat
     1141 cactgtttgg taatgctttg gatttatcac ccgaattaag aaataaaatt caaattgtta
     1201 cgaaatgtgg tatcattttg ccttctaagc aatttgattt tacaaatgga catcgttatg
     1261 atttgagtag taagcacatc gtgaaatctg ttgaacagtc attaatcaat ttgaatgtag
     1321 attatttaga tagtctactc attcatcgtc cttcaccgtt gatggatcca gaacaagttg
     1381 ctgatgcatt aactaaactt gttaaacaag gtaagttgaa gtcattcggg gtgtcgaatt
     1441 ttaatcattc acaataccaa ttgttaaatc aatatattat gaaagaaaga ctacatatta
     1501 gcatcaatca attagaatta tcgccatatc acgttgatag tttacaagat ggaacaatgg
     1561 attcaatgta tcaaaaccat gttcaaatta tggcttggag tccttttgca ggcggtaaaa
     1621 ttttcgacaa ggaagatatt aaagcgcaac gtattatgaa agttgttcaa tcaatagctg
     1681 acaaatatgg tgtgagtgac acagctgtga tgatagcatg gttagtaaaa ataccgcata
     1741 gtatcatgcc gatacttgga acaagtcagt taaagcgtat tgatcaagca atcgaagggc
     1801 tacaacttaa tttagatgat cagtcgtggt ttgacattta caccgctatt atcggacaag
     1861 atattccgta aacttattta cttttaaatc ataaaggagc atatcatgac aaacgaagat
     1921 aaacgtttcg aaca
//
LOCUS       DAOBBH010000273         1911 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.275, whole genome shotgun sequence.
ACCESSION   DAOBBH010000273 DAOBBH010000000
VERSION     DAOBBH010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.275"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            102..1739
                     /locus_tag="P6L01_002543"
     CDS             102..1739
                     /locus_tag="P6L01_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDP3031579.1"
                     /translation="MDKELWIERANDSLVKHFYEQQSDIEQREGFESKLTFGTAGIRG
                     KFGLGEGRLNKFTIEKLALGLARYLNAQTNSPTIVIHYDIRHLSTEFAQIIANVLANH
                     QITVYLPDTYKTTPELSFAVRNLNTTAGIMITASHNPKDYNGIKVYGSDGAQLSTDAS
                     ELASRYIEEVGDPLQIDIPISKQNTSYIKPFPKSVTDDYMKHIQNMIGYIPKSDLQVV
                     FTSLHGTSVPIVPELLKSLNFNQFNLVDAQCKPDPNFSSVQSANPEDHRAFDQAVELA
                     NKSHADLLISTDPDADRLGIAERDAHGHITYFNGNQIGALLLNYRIQQTSQLRHRLMI
                     QSIVSSELTKSLARYNNVKYKEVLTGFKFIAQEIRQLDDHQNMIFAFEESYGFLSEPF
                     VRDKDAVQIVPLIIKYASELKLYGKTLKDELEQIYQTVGRHEDTLFSHTLEGLEGKKK
                     IESIMTHFRSNPPQEIQGLKVKAIEDYLTSEVYHLDKDTTSQINSSKSNVIRVLFDEG
                     FIALRPSGTEPKIKLYVSLKCPDFDDVAQKINAMIFS"
BASE COUNT          654 a          295 c          327 g          635 t
ORIGIN      
        1 tttggggcgt atttctttat aattcattac ataaatgtaa gggctttagt tttcatgttt
       61 tattaagtct aactgagatt ttgaaaggat gtttagcaac aatggataaa gaattatgga
      121 tagaaagagc taatgatagt ttagttaaac atttttatga gcagcaatct gacattgaac
      181 agcgagaagg ttttgaaagt aaattaacat ttggtactgc gggtatacgc ggaaaattcg
      241 gtcttggtga aggtcgactt aataagttta ctattgaaaa attggcatta ggtttagcgc
      301 gttatttaaa tgcccaaaca aacagtccaa caatagtcat tcattatgat attagacatc
      361 tttcaactga attcgcccaa attattgcta atgtattagc aaaccatcaa ataacagttt
      421 atttacctga tacatataaa acgacaccgg aattatcgtt cgcggttcgc aatcttaata
      481 ctactgccgg cattatgatt acagcaagtc ataatccgaa agactataac ggcattaaag
      541 tatatggttc tgatggcgcg caattatcga ctgatgcatc tgagcttgca agtcgttata
      601 tcgaagaagt tggcgatcca ttacagattg atatacctat ttcaaaacaa aatacatctt
      661 atatcaaacc ttttccgaaa tctgtaactg atgactatat gaaacacatt caaaatatga
      721 ttggctatat tcctaaatct gatttacaag ttgtgttcac tagtttgcat ggtacaagtg
      781 ttccaattgt acctgagtta ttaaaatctt tgaatttcaa tcaatttaat cttgtagatg
      841 cacaatgtaa acctgatccg aattttagtt cagttcaaag tgctaatcct gaagatcatc
      901 gcgcttttga tcaagcagtc gaacttgcaa ataaaagtca tgcagactta ttaatatcaa
      961 ctgacccaga tgcagatcga cttggtattg ctgaacgtga tgcacatggt catatcacat
     1021 attttaatgg caaccaaatt ggtgcgctgt tacttaatta tcgtattcaa caaacgtctc
     1081 aattacgtca ccgcttaatg attcaatcta ttgtaagtag tgagttaaca aagtcattag
     1141 cacgctataa taatgttaaa tacaaagagg tactgacggg ctttaaattt attgctcagg
     1201 aaataagaca actggatgat catcaaaata tgattttcgc atttgaagag agttacggtt
     1261 ttctatcaga gccttttgta cgtgataaag atgccgtaca aattgtgcca ctcattataa
     1321 aatacgcttc tgagttaaaa ttatatggca aaacattgaa agatgaatta gaacagattt
     1381 atcaaactgt tggtagacat gaggatacat tattctcgca tacgctagaa ggattagaag
     1441 gtaagaaaaa aattgaatca attatgacac atttccgttc aaatccaccg caagagattc
     1501 aaggactgaa agtgaaagca attgaagatt atttaactag cgaggtttat catttggata
     1561 aagatacaac gtcacagatt aattcttcta aatcaaatgt tattcgtgtc ttatttgacg
     1621 aaggatttat cgctttacgt ccttctggta ctgaacctaa aattaaatta tatgtgtcgt
     1681 taaaatgtcc ggattttgat gatgtagctc aaaaaattaa tgcgatgatt tttagctaat
     1741 taaaaatttt atacatcgaa atattacatt tcattttata cttatcagcc cttttgaaac
     1801 tagaatcctt atgttttcgc gttttgaagg ggctaatttg taataatttt tcaaaagtat
     1861 attagaaaat taattttcat gtatttttca tattaagcgg taataatata a
//
LOCUS       DAOBBH010000274         1890 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.276, whole genome shotgun sequence.
ACCESSION   DAOBBH010000274 DAOBBH010000000
VERSION     DAOBBH010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.276"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..502
                     /locus_tag="P6L01_002544"
     CDS             <1..502
                     /locus_tag="P6L01_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1829 domain-containing protein"
                     /protein_id="HDP3031580.1"
                     /translation="KYNDKTHEIFVQTNFKNFNKSKHNLLQCLIFVNDMYLLSNPKSQ
                     NIFTEDVANKLDEHNIYYGRDIPIIGSSGVVHNFDFFISAKKNQKEKFINVISNPNNS
                     MIIKSKITDAMQAKKIKRHRQNEFIFILNDSKKEINEHNKNLLHENYISTIDYSELDE
                     KIGLLI"
     gene            1336..1830
                     /locus_tag="P6L01_002545"
     CDS             1336..1830
                     /locus_tag="P6L01_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031581.1"
                     /translation="MRRLLSLLLASTMILVACGNANNENKKKEDEKKSEVKKEATKNN
                     DKPKNEKKNQDINKNNNEQVQNVNNKQPVLNNNSNHNNQQQSNKNNYTVIENGNTVTE
                     IYNGQSHTTTNNPIREEYVEGETDPIYKREYKHFYTPEEAQRAQENSDQILREMGLEP
                     ERYE"
BASE COUNT          816 a          243 c          259 g          572 t
ORIGIN      
        1 taaatataac gataaaactc acgaaatttt tgttcaaact aactttaaaa attttaataa
       61 atcgaaacat aatttattac agtgccttat atttgttaat gatatgtact tactttctaa
      121 tcctaagtca cagaacatat ttacagaaga tgttgcaaac aaattggatg aacataacat
      181 ttattacgga agagatatac ctattatagg aagcagtggt gttgttcata atttcgactt
      241 ttttattagc gctaagaaaa atcaaaaaga aaaatttatc aatgttattt ctaaccctaa
      301 taattctatg attattaagt cgaaaataac ggatgctatg caagcaaaaa aaataaaaag
      361 acacaggcaa aatgagttta tttttatttt aaatgactca aaaaaagaaa taaatgaaca
      421 taataaaaat cttcttcatg aaaactatat tagtacaata gattatagcg aattagatga
      481 aaagataggt ttattgattt aatatttata gacgtgataa tatcaatgtt tatattaaat
      541 gaaacgaatt tgaaaatttc gaaacagctt aagatagcaa attgaatagt cttatagata
      601 atgcagaatc gtctacacat agttaaacaa attatttgag aataattatt gttaaactat
      661 gtggataggc gattttattt tgacaagagt cagaagaaca tatattaagg atttcaaatt
      721 acaaatggct aaactttatg aaaatggtat gactagaatc gaaattatac gcgaatacga
      781 tttaacactc tcaatcttct cgaactgaat aaatactggg tcattcaatc attaagacaa
      841 cttaataagt gatgatatat gttcatacca ttttgcaaat ataaccagat actctcaaca
      901 aaatgtagag ttcaaaatta aaaatgtcaa cattatagta aactaacata gtcccctgtt
      961 catattcttc aatctttaaa tcttatcgaa caaataaaag cagataattt tttaatttga
     1021 acgttttatt ttagaacaaa aaagagcaag gttaacgagt cagaaaatct ttttcttgat
     1081 acgtatatga cacttgctct tttaagaatc tgaatatttc acgctatttc cattaaatta
     1141 gcggagaatg aatgatatct ttatactaat aaatttaact ttttaacgta taaaattaat
     1201 gcattgtact aaagtttcaa ttattttata attgtatgaa tgaatcgtat acacttaggt
     1261 agtcaaatct aagaatttag atattgttta attttaaaac tatcgattca catataaata
     1321 aaaggagata acagcatgag gagattactc agtctattac tagctagtac aatgatttta
     1381 gtcgcatgtg ggaatgctaa caatgaaaat aagaaaaagg aagacgaaaa aaaatcagaa
     1441 gtaaaaaaag aagctacgaa aaataatgat aaaccaaaga acgaaaagaa gaatcaagat
     1501 ataaataaaa acaataatga gcaagttcaa aatgtcaata ataaacaacc agtcttaaat
     1561 aataatagta atcataacaa tcaacaacaa agcaataaaa ataattacac agttatagaa
     1621 aacgggaata cagttacaga aatttataat ggacaatcac acacaactac aaataaccct
     1681 attagagaag aatatgtaga gggagaaaca gacccgatat ataaaagaga gtataagcat
     1741 ttttatacac ctgaagaagc tcaaagagca caagaaaata gcgaccaaat tttaagagaa
     1801 atgggtctcg aaccagaaag atatgaataa aatcaaacta aataatttcg ggtagccccc
     1861 ctacccttat tatttttttg ctaagaagta
//
LOCUS       DAOBBH010000275         1865 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.277, whole genome shotgun sequence.
ACCESSION   DAOBBH010000275 DAOBBH010000000
VERSION     DAOBBH010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.277"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..400)
                     /locus_tag="P6L01_002546"
     CDS             complement(<1..400)
                     /locus_tag="P6L01_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDP3031582.1"
                     /translation="MSNTQTKNVPELRFPGFEGEWEEKKLGNLTTKIGSGKTPKGGSE
                     NYTNKGIPFLRSQNIRNGKLNLNDLVYISKDIDDEMKNSRTYYGDVLLNITGASIGRT
                     AINSIVETHANLNQHVCIIRLKKEYYYNFFG"
     gene            complement(393..>1865)
                     /locus_tag="P6L01_002547"
     CDS             complement(393..>1865)
                     /locus_tag="P6L01_002547"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDP3031583.1"
                     /translation="MDASEFRNYILGLIFYRFLSEKAEQEYADALSGEDITYQEAWAD
                     EEYREDLKAELIDQVGYFIEPQDLFSAMIREIETQDFDIEHLATAIRKVETSTLGEES
                     ENDFIGLFSDMDLSSTRLGNNVKERTALISKVMVNLDDLPFVHSDMEIDMLGDAYEFL
                     IGRFAATAGKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLLRVGKETQ
                     VYRYFGQERNNTTYNLARMNMLLHDVRYENFEIRNDDTLENPAFLGNTFDAVIANPPY
                     SAKWTADSKFENDERFSGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPHGVLFRG
                     AAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLFIDASND
                     FEKGKNQNHLSDAQVERIIDTYKRKATIDKYSYSATLQEIADNDYNLNIPRYVDTFEE
                     EAPIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE"
BASE COUNT          501 a          390 c          305 g          669 t
ORIGIN      
        1 gtccaaaaaa attataataa tactcttttt ttaatctaat aatacataca tgttgattta
       61 aattagcatg cgtttcaact atcgaattaa tggctgttct acctattgat gctcctgtaa
      121 tatttaaaag aacatcacca tagtacgttc tactattttt catctcatca tctatatctt
      181 tactaatata aactaagtca ttaagattta atttaccatt tctaatattt tgactcctta
      241 aaaatggtat gcctttgttt gtatagtttt cacttccacc tttgggagtc tttccactac
      301 ctattttggt agtaagattc cctaacttct tctcttccca ttcgccctca aatcctggga
      361 atctcaactc tggcacattt ttcgtttgtg tattactcat ctttcaacac cccaagttct
      421 ttcaggtatg cattgatttc ttgttcaatt tctgcgattt ctttgtcgat atttttcaaa
      481 tcttgttgga cttgatctaa atcaattggt gcttcttctt cgaatgtatc aacatatcgc
      541 ggtatgttta agttgtaatc gttatcggcg atctcttgta atgtcgcgct gtagctatat
      601 ttatcaatcg ttgctttacg cttatatgtg tctataatac gttcgacttg ggcatcgctt
      661 aaatggtttt gattttttcc tttttcaaaa tcattggatg catcgataaa tagtacgttg
      721 tcgtcttgtt ggcgacattt tttaaatact aaaatacatg ttggaatact tgtcccatag
      781 aaaatattgg ctggtaaccc aatcacggct tctaagtagt tcttttcttc tattaaatag
      841 cgacgaatca caccttctgc ggcaccacgg aataatacac catgtgggag tacgactgcc
      901 atggtacctt catcgtctag gtaatgtacc atgtgttgaa taaaggcaaa gtctgctttg
      961 gattttggcg caagcttgcc gtaaccactg aatcgttcat cattttcaaa ttttgaatct
     1021 gctgtccatt tcgcactgta tggtgggttc gcaataaccg catcaaatgt attgcctaaa
     1081 aaggctggat tttccaatgt gtcatcatta cggatctcga agttctcata acgcacatca
     1141 tgtaataaca tattcatgcg tgctaagttg tatgtagtat tgttacgttc ttgtccgaaa
     1201 taacgataca cttgcgtttc tttaccaaca cgtaacaaca atgaaccgga accacatgtt
     1261 gggtcgtaca cgtgacgtaa tttatcttta ccgtctgtga caatcttcgc cagtatctta
     1321 gatacttgtt gtggtgtata gaactcgcct gcttttttac ccgctgtcgc cgcaaagcgc
     1381 ccgattagga attcatatgc atcacctaac atatcaattt ccatgtcact gtgaacgaat
     1441 ggtaagtcgt caagattaac catgacttta gagattaaag cagtacgttc tttgacattg
     1501 ttacctagtc gcgttgaact caaatccata tcgctgaaca gcccgataaa gtcattttca
     1561 ctttcttcac ctaatgtaga tgtttcaact ttgcgaatcg ccgtcgctag atgttctata
     1621 tcgaaatctt gcgtttcaat ttcacgaatc atcgcactga ataaatcttg tggctcaatg
     1681 aagtaaccga cttgatcaat taattctgct tttaagtctt cacgatattc ttcatctgcc
     1741 catgcttctt gatacgtgat gtcttcacct gacaaggcat ctgcatattc ttgttcggct
     1801 ttttcagata agaagcgata gaaaatcaag cctaaaatgt aattacggaa ttcactcgca
     1861 tccat
//
LOCUS       DAOBBH010000276         1862 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.278, whole genome shotgun sequence.
ACCESSION   DAOBBH010000276 DAOBBH010000000
VERSION     DAOBBH010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.278"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..568
                     /locus_tag="P6L01_002548"
     CDS             <1..568
                     /locus_tag="P6L01_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HDP3031584.1"
                     /translation="LGKTLVASALVLTTLGTGLHSSYLGLNTNKVVKTAKAEEKMTNG
                     QLWKKVKDSLIDSNIISGNENEEITVTYVNKTGYSSSVSAYGNNNDDFSSTPSNFSKL
                     KEIDLKKDNVPSDDFNTTVSGEDSWKTLTSKLKEKGLVTDGQTVTIHCNDKSDNTKSS
                     VSGKVGADLTSGNGTTFKKRFIDKITID"
     gene            complement(1041..1329)
                     /locus_tag="P6L01_002549"
                     /pseudo
     CDS             complement(1041..1329)
                     /locus_tag="P6L01_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500445.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BsaG protein"
     gene            1515..>1862
                     /locus_tag="P6L01_002550"
     CDS             1515..>1862
                     /locus_tag="P6L01_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031585.1"
                     /translation="MHLKLNDEDLNYLVNLSKEILEQYNIKLDETCKGDINITSCNSE
                     YFFTLSYFLKPGKATINFRECRYNYSLLRLNLNDGFHKNSNNEKIRGNRINIFSEKEF
                     IQKADGATHMKAYP"
BASE COUNT          742 a          287 c          245 g          588 t
ORIGIN      
        1 tttaggtaaa acattagtag caagtgcttt agtattaaca acattaggaa caggtttaca
       61 ttcttcatac ttaggattaa atacaaataa agttgttaaa acagcaaaag cagaagaaaa
      121 aatgacaaat ggtcaattgt ggaaaaaagt taaagattca ttaattgatt caaatattat
      181 tagtggtaac gaaaatgaag agattacagt aacatatgtt aataaaaccg gatattcttc
      241 aagtgtttct gcctatggta ataataatga cgatttttca agtactccaa gtaatttttc
      301 taaacttaag gaaattgact tgaaaaaaga taatgtaccg tcagatgatt ttaatactac
      361 tgttagtggt gaagatagtt ggaaaacact tacatctaaa ttaaaggaaa aaggtttggt
      421 tacagatgga caaacagtaa ctattcattg taatgataag tctgacaata caaaatcatc
      481 tgtttcaggt aaagtaggag cagatctgac aagtggcaat ggaactacat tcaaaaaacg
      541 ctttattgat aaaatcacaa ttgattaaac cttaaactca aatttcctta attcataaaa
      601 ccttaataac taaccctttc ttacaaacca cctagcccat aatatattaa aacgtcattt
      661 ttatttcaat tagcggaaaa taaaaaaacc attccgcatt aactttggca agttggaatg
      721 gttaaaaaat attttagaag ccagcgtttg gcttgctttg acgacatgtt cgcgcatgtc
      781 gtcttatttt tacatttata taataacacg caattcaata aaatgtctaa tcaataaacc
      841 tcaattacat tcaaattcaa aaactttgca cctaatattc atcgcttaaa taaacattac
      901 gtttaattaa ccagacattg tcaatcgcgt ctaatacccc tgctgtccga atacaaaatt
      961 attttgtatt catatttatc tgttctcttt cctccctttt aaacgcaact aaatttcttt
     1021 taccattagc gccccaatct ttaatcttta attgttctac cgctccattt attaaatcct
     1081 ttaaagagta aaactgctaa tagcaacgtg ataataatat agatcgccaa tgttaatgta
     1141 actggtatac tcccttcgat aaacatataa acgtaacacg tagcatatgt gattggtaaa
     1201 tagaaccacg aatgatctcc aagcacttct aatccaaaat aaacgttaaa aataaacatt
     1261 aaaactccga caacaatagc cattacatct ttaatgaaaa atatatctaa cattatcaca
     1321 aattattaat aatgttaaaa atgacggtac taaataaatt aataaatatt ccattgtcat
     1381 gtattgctaa acgattatga cgctaaaaag gaataattat taagtttata gaaaacatct
     1441 gtataactta ctacaaatac tgaaaataat ataaggattt aagatcgtaa aatctaaact
     1501 aaggagatgt ttaaatgcat ttgaaactga acgatgaaga tttaaattat ttagtaaatc
     1561 taagtaaaga aatacttgaa caatacaaca ttaaattaga cgaaacttgt aagggagata
     1621 ttaacataac atcatgtaat tctgaatact ttttcacttt aagctatttt ttaaagcctg
     1681 gaaaagcaac tataaatttc agagaatgcc gttacaacta tagcttgtta cgtttaaatc
     1741 ttaatgacgg ttttcataaa aactctaata atgagaaaat aaggggaaat agaattaata
     1801 tattttctga aaaagaattt atacaaaaag ctgacggcgc aacccatatg aaagcatatc
     1861 ct
//
LOCUS       DAOBBH010000277         1810 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.279, whole genome shotgun sequence.
ACCESSION   DAOBBH010000277 DAOBBH010000000
VERSION     DAOBBH010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.279"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..453
                     /locus_tag="P6L01_002551"
     CDS             <1..453
                     /locus_tag="P6L01_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDP3031586.1"
                     /translation="LYKFFKENEKKYQYTGFTKEAINKTQNVGYQNEYFYITYLSRNL
                     KEYRKYYEPLIHKNDKEFKEGMQRARKELDYTANSNTVATLFSTNDKKNRKEKINNVI
                     DLSEKIERTKDMPIKNTITTQLGNKLIGTKKARFDDKKVVSFGAFEDE"
     gene            446..>1810
                     /locus_tag="P6L01_002552"
     CDS             446..>1810
                     /locus_tag="P6L01_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031587.1"
                     /translation="MNKINDRDLTELSSYWVYQDINKDNDFTVNGKRFKQVDEYNDNG
                     NKNKKGASDLKIYELLDEKGKPTGEQTMIYQGTSNEAINPNNPLKSLDIGDDWLQNAK
                     LMDNSNKSTDYLKQSDEFADLYRDKLNDANKLSKYNFTQKYGVSPNNYKNKTIVADGG
                     NSEGGAGAKYQGAKHPNEKVVATDPAMVPYAAWQKFARPRFDNMISFNSTNDLLTWLQ
                     DPFIKDMPGKRVNISDGVPRLDALIDSHVGYKRKLNRKDNTYDTVPLIKIKSVKDTEI
                     KNGKKVKKTINITLDMDGRIPINVWTGDSIARSGRGTLIKLNLENLDALSKLITGETS
                     GMLAECVIFLNESFNISENENKNFADRKQQLSEGFKDKINLFQLEEMERTLISKINSL
                     EEVADETIESISAVKHLLPDFALDALKERINELFKGIKSFIEKVYDSIDNEILEIFKN
                     IDHDF"
BASE COUNT          776 a          207 c          325 g          502 t
ORIGIN      
        1 ttatataaat tcttcaaaga aaatgaaaag aaatatcaat atacaggctt tacaaaagag
       61 gcaattaaca agacacaaaa cgttggatat caaaatgaat acttttatat cacatattta
      121 tcaagaaact taaaagaata tcgtaaatat tacgaaccgt tgattcataa aaatgataaa
      181 gaatttaaag agggtatgca acgagctaga aaagagttag attatactgc taatagtaat
      241 acagtagcaa cgttgtttag tacgaatgat aaaaaaaata gaaaagaaaa gataaataat
      301 gtaatagatt tatccgagaa aattgaaaga acaaaagata tgccaatcaa gaatactata
      361 actactcaat taggaaataa acttattggc acaaaaaaag ctcgttttga tgataagaaa
      421 gtagtgtcgt ttggagcatt tgaagatgaa taaaattaat gatagagact taacagaatt
      481 gagtagttac tgggtttatc aagacatcaa taaagataat gattttacag ttaacggaaa
      541 aagatttaag caggttgatg aatataatga taatggaaat aaaaataaaa aaggtgcctc
      601 agatttaaaa atttatgaat tgttggatga aaaaggaaaa ccaactggtg aacaaaccat
      661 gatttatcaa ggaacatcta atgaggcaat aaaccctaat aatccattaa aatctttaga
      721 tatcggagat gattggttac aaaatgcgaa attaatggat aatagtaata agtcaacgga
      781 ttatcttaag caatcagacg aatttgcaga tttatataga gacaaactaa atgacgctaa
      841 taaattaagt aagtataact ttacacaaaa atatggtgtt agtccaaata attacaaaaa
      901 caaaaccatt gtggcggatg gcggtaattc ggaaggcggt gcaggagcaa aatatcaagg
      961 agcgaaacat ccaaatgaaa aagttgttgc tactgaccca gcaatggtac cttatgctgc
     1021 ttggcagaaa tttgctagac cacgctttga taatatgatt agttttaata gtaccaacga
     1081 tttattaaca tggttacaag atccattcat caaagatatg ccaggaaaac gcgttaacat
     1141 tagtgatggt gtgcccaggt tagatgcttt aatagacagc catgtaggtt ataaaaggaa
     1201 gttaaataga aaagacaaca catacgatac tgtaccacta atcaaaatta agtcggtaaa
     1261 agatacagaa attaaaaatg gaaaaaaagt aaaaaagact attaacataa cattagatat
     1321 ggatgggcga attccgataa atgtttggac aggggattcg attgcacgtt ctggaagagg
     1381 aactttaatt aaacttaatt tagaaaatct tgatgcgttg agtaaactga ttactggtga
     1441 aactagtggt atgttagcag aatgcgtaat ctttttaaat gaaagtttta acatctcaga
     1501 aaatgaaaat aaaaattttg cagatagaaa gcaacaatta tcagaaggat ttaaggataa
     1561 gattaactta tttcaattag aagaaatgga aagaacttta attagtaaaa taaactcact
     1621 tgaagaagtt gcagatgaaa caatagaaag tattagtgct gttaaacact tattacctga
     1681 ttttgcattg gatgcattaa aagaaagaat taatgagttg tttaaaggta taaaatcttt
     1741 tatagaaaaa gtgtatgata gtatagataa tgaaatttta gaaattttca aaaatataga
     1801 tcacgacttc
//
LOCUS       DAOBBH010000278         1804 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.280, whole genome shotgun sequence.
ACCESSION   DAOBBH010000278 DAOBBH010000000
VERSION     DAOBBH010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.280"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            32..532
                     /locus_tag="P6L01_002553"
     CDS             32..532
                     /locus_tag="P6L01_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDP3031588.1"
                     /translation="MSAIIEAKKQLVDEIAEVLSNSVSTVIVDYRGLTVAEVTDLRSQ
                     LREAGVEYKVYKNTMVRRAAEKAGIEGLDEFLTGPTAIATSSEDAVAAAKVISGFAKD
                     HEALEIKSGVMEGNVITAEEVKTVGSLPSHDGLVSMLLSVLQAPVRNFAYAVKAIGEQ
                     KEENAE"
     gene            575..943
                     /gene="rplL"
                     /locus_tag="P6L01_002554"
     CDS             575..943
                     /gene="rplL"
                     /locus_tag="P6L01_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDP3031589.1"
                     /translation="MANHEQIIEAIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAA
                     GGADAAAEKTEFDVELTSAGSSKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEALPK
                     EEAEKLKEQLEEVGATVELK"
     gene            1118..1726
                     /locus_tag="P6L01_002555"
     CDS             1118..1726
                     /locus_tag="P6L01_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDP3031590.1"
                     /translation="MSHYYDEDPSVISNEQRIQYQLNHHKIDLITDNGVFSKDKVDYG
                     SDVLVQTFLKAHPPGPSKRIADVGCGYGPIGLMIAKVSPHHSITMLDVNHRALALVEK
                     NKKLNGIDNVIVKESDALSAVEDKSFDFILTNPPIRAGKETVHRIFEQALHRLDSNGE
                     LFVVIQKKQGMPSAKKRMNELFGNVEVVNKDKGYYILRSIKA"
BASE COUNT          669 a          240 c          365 g          530 t
ORIGIN      
        1 aagcaccaaa aatttaaatg gaggtgtctg aatgtctgct atcattgaag ctaaaaaaca
       61 actagttgat gaaattgctg aggtactatc aaattcagtt tcaacagtaa tcgttgacta
      121 tcgtggatta acagtagctg aagttactga cttacgttca caattacgtg aagctggtgt
      181 tgagtataaa gtatacaaaa acactatggt acgtcgtgca gctgaaaaag ctggtatcga
      241 aggcttagat gaattcttaa caggtcctac tgctattgca acttcaagtg aagatgctgt
      301 agctgcagcg aaagtaattt ctggatttgc taaagatcat gaagcattag aaattaaatc
      361 aggcgttatg gaaggcaatg ttattacagc agaagaagtt aaaactgttg gttcattacc
      421 ttcacacgat ggtcttgtat ctatgctttt atcagtatta caagctcctg tacgcaactt
      481 cgcttatgcg gttaaagcta ttggagaaca aaaagaagaa aacgctgaat aatttttagc
      541 gtaaaaaaat taaaaataat ggaggaatta taaaatggct aatcatgaac aaatcattga
      601 agcgattaaa gaaatgtcag tattagaatt aaacgactta gtaaaagcaa ttgaagaaga
      661 atttggtgta actgcagctg ctccagtagc agtagcaggt gcagctggtg gcgctgacgc
      721 tgcagcagaa aaaactgaat ttgacgttga gttaacttca gctggttcat ctaaaatcaa
      781 agttgttaaa gctgttaaag aagcaactgg tttaggatta aaagatgcta aagaattagt
      841 agacggagct cctaaagtaa tcaaagaagc tttacctaaa gaagaagctg aaaaacttaa
      901 agaacaatta gaagaagttg gagctactgt agaattaaaa taattcaagt atcttaaact
      961 taataatcaa agttttatag caagtattgc tataatataa tgattctttg agaagttaaa
     1021 accccgttat tttgataacg gggttttatt catttaaaga ctgagtgaaa tgttataatt
     1081 ataatgacga gttacaaagt gaagatgagg tggaataatg agtcattatt acgatgaaga
     1141 tccaagtgta attagcaatg aacaacgtat tcaatatcaa ttaaaccatc ataaaattga
     1201 tttaataact gataacggag tgttttcgaa agataaagta gattatggtt cagatgttct
     1261 tgttcaaact tttttaaaag cgcatccacc tggtccaagt aagcgaattg ccgatgttgg
     1321 ttgtggttac ggaccaattg gtttgatgat tgctaaagta tcaccacatc attcaattac
     1381 aatgctagat gttaatcaca gagcgctagc cttagttgaa aaaaacaaaa aattaaatgg
     1441 tattgataat gtgatcgtaa aggaaagtga tgctttgtct gctgtggaag acaaaagttt
     1501 tgattttatt ttaaccaatc caccaataag agcagggaaa gaaaccgtgc atcgtatatt
     1561 cgagcaagca ttacatagat tagactcgaa cggtgaacta ttcgttgtaa ttcagaagaa
     1621 gcaaggtatg ccatctgcaa agaaaagaat gaatgaactt tttggaaatg tagaagtggt
     1681 aaataaagat aaaggatatt acattctgag aagtataaaa gcttgaaatg aaatggatat
     1741 tctgttatag ttatataatg taaaaattta tgttcaataa gtgtgtactt ttacgttaaa
     1801 taga
//
LOCUS       DAOBBH010000279         1788 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.281, whole genome shotgun sequence.
ACCESSION   DAOBBH010000279 DAOBBH010000000
VERSION     DAOBBH010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.281"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            61..807
                     /locus_tag="P6L01_002556"
     CDS             61..807
                     /locus_tag="P6L01_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031591.1"
                     /translation="MNRIEKHAKNTFIILMLIMLFWIFMSFIFQKLLFPPSKNNLTTY
                     EALKYYTHLKGYYGLDHISKGIAYIACVLIPFNFFFRFNDIKKDNNYNNIISTLFLLL
                     YFLVNGISLIIQGFTAEFTISLISESNIHNNHEFAVNLFRYVIQEGGISFSTYLVCNF
                     SIIMWLFFSCSLLKERKPVVRCLPLIISCLKLILILLFLLSILLVIYQTQSAQILFIF
                     IDFLNFVALILVYLCTNPNNRGIDKIACVK"
     gene            1037..>1788
                     /locus_tag="P6L01_002557"
     CDS             1037..>1788
                     /locus_tag="P6L01_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDP3031592.1"
                     /translation="MRFVFDIDGTLCFDGRLIDQTIIDTLLQLQHDGHELIFASARPI
                     RDLLPVLPSVFHQHTLIGANGAMISQQSKISVIKPIHTDTYHHILKIIQKYELDYIID
                     DDWNYAAQLDAENAIFERLDPHKLASCIDVANIDTPIKIILLNIDPAQITTILDELNK
                     YHQELEMIHHSNEYNIDITAQNINKYTALQYIFDADVKYIAFGNDHNDIFMLQHASSG
                     YIIGPSEAYTHAILKLDKIKHINNNTQAICKV"
BASE COUNT          642 a          251 c          246 g          649 t
ORIGIN      
        1 atataatata aaataatggt tatctgagta tgaaatgcaa gtaagtgagg tagttaaaaa
       61 atgaatcgaa ttgaaaaaca tgccaaaaac acttttatta ttctgatgct cattatgtta
      121 ttttggattt ttatgagttt tattttccag aaattattat ttccaccttc gaaaaataat
      181 ttaactacat atgaagcatt aaaatattat acacatttaa agggatatta cggtttggat
      241 catatatcaa aaggaatagc atacattgcc tgtgtgttaa ttccattcaa tttctttttt
      301 agattcaatg acatcaaaaa agataataac tataataata ttataagtac gttattccta
      361 ttattatact ttttagttaa tggaatttcg ttgattattc aaggattcac tgcagaattt
      421 acaattagtt taattagtga gtcaaatatt cataataatc atgaatttgc tgtgaactta
      481 tttagatatg tcatacaaga aggtggtatt tcttttagta catatctagt gtgtaacttt
      541 tctataataa tgtggttatt cttttcgtgt tctttattaa aagaacgaaa accagttgtc
      601 agatgtttac cattaattat atcttgttta aaattaatat taatcctact atttttactt
      661 tcaatacttt tggtcatata tcaaactcaa tctgcacaaa tcttatttat ttttatagat
      721 tttctaaatt ttgtcgcgtt aattttagtt tatttatgta ctaatcctaa taatagaggt
      781 attgataaaa tagcatgtgt taaataaaaa gttaatagta actacaaaca tactttacta
      841 ataatcaaaa cgacttatac aaaatcgaag ttatataagt ccccttatgg aatttcacat
      901 tctagtttac ataatatata ttataggaag taatgtgtgt gacgcaaaag gtaccctaca
      961 tcataatcat tatctaatat cgtcacataa cttacttatg ctataatcat ggtattatat
     1021 tgtttggagt gatttgatga gatttgtctt tgatattgat ggtacgcttt gttttgacgg
     1081 ccgattaatt gaccagacta ttattgatac attgttacaa ttacaacatg atggtcatga
     1141 acttatattt gcatcagcac gtccgattcg tgatttgttg ccagttttac catcagtatt
     1201 tcatcagcac acattaattg gcgcaaatgg tgctatgatt tcacagcaat caaagatttc
     1261 tgttatcaaa ccaattcata cagatacata tcaccatatc ttaaaaataa ttcaaaagta
     1321 tgagttagat tatattattg atgatgattg gaattatgct gcacaacttg acgctgagaa
     1381 cgcgattttt gagcgtttag atccacataa gctggccagt tgtattgatg ttgcaaatat
     1441 cgacacacca atcaagatca ttttattaaa tatagacccg gcacaaatta caactatatt
     1501 agacgagcta aataaatacc atcaagaatt ggaaatgatt caccattcaa atgagtataa
     1561 cattgatata acagcgcaaa atattaataa atatactgca ttacaatata tatttgatgc
     1621 agatgttaaa tatatagcat ttggtaatga ccacaatgat attttcatgt tacaacatgc
     1681 tagtagtggc tatattatag gaccatcaga agcatacaca cacgcaatat tgaaacttga
     1741 taaaatcaaa cacatcaata ataatacaca agctatttgc aaagtctt
//
LOCUS       DAOBBH010000280         1786 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.282, whole genome shotgun sequence.
ACCESSION   DAOBBH010000280 DAOBBH010000000
VERSION     DAOBBH010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.282"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(7..1245)
                     /gene="hflX"
                     /locus_tag="P6L01_002558"
     CDS             complement(7..1245)
                     /gene="hflX"
                     /locus_tag="P6L01_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDP3031593.1"
                     /translation="MAQQQIHDTKNKLEKAVLVGVHAQDDKQFNFESTMEELSSLSET
                     CQLEVLGQITQNRDRVDRKYYVGKGKIEEIQAFIEFKDIDVVITNDELTTAQSKSLNE
                     ALGVKIIDRTQLILEIFALRARSKEGKLQVELAQLDYLLPRLQGHGKSLSRLGGGIGT
                     RGPGETKLEMDRRHIRTRMNEIKHQLRTVEEHRERYRNKRNQNQVFQVALVGYTNAGK
                     SSWFNVLANEETYEKDQLFATLDPKTRQIQINDGFNLIISDTVGFIQKLPTTLIAAFK
                     STLEEAKGADLLVHVVDSSHPEYRTQYDTVNDLIKQLDMSHISQIVIFNKKDLCDHAS
                     NRPASDLPNVFVSSKNDGDKLLVKTLFIDEIKRQLTYYDETIATNNADRLYFLKQHTL
                     VTELKYDEIENVYRIKGFKK"
     gene            1357..>1786
                     /locus_tag="P6L01_002559"
     CDS             1357..>1786
                     /locus_tag="P6L01_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDP3031594.1"
                     /translation="METIYDFVVETNKGVTYKLDAYKGDVMLIVNTASECGFTSQFEG
                     LQSLYEKYKDQGFVILGFPCNQFGGQEPGSGEEAAQNCKLNYGVTFPMHQKIDVKGEH
                     QLPLFRYLTAAQHGFFNEKIKWNFTKFLVDREGNVVKRFAP"
BASE COUNT          572 a          310 c          262 g          642 t
ORIGIN      
        1 cttttattat tttttaaatc cttttatacg ataaacattt tcaatttcat catatttaag
       61 ttcagtcact aatgtatgtt gttttagaaa atataatcga tctgcattat tcgtcgcaat
      121 tgtctcatca taataagtta attgcctttt gatttcatca ataaataacg tcttaacaag
      181 taatttatca ccatcatttt tagaagaaac aaaaacatta ggcaaatcac ttgctggacg
      241 atttgatgca tgatcacata agtccttttt attaaaaata actatttgag aaatatgact
      301 catatctaat tgtttgatta aatcattaac tgtgtcatac tgcgtacggt attcaggatg
      361 gctactatct acgacatgta ctaataaatc tgcaccttta gcctcttcta aagttgattt
      421 aaaagctgca attaacgtcg taggtagttt ctgtataaaa ccaacagtat ctgaaataat
      481 taaattaaat ccatcattta tttgaatttg tcgtgtttta ggatctaacg ttgcaaataa
      541 ttgatccttt tcatacgtct cttcatttgc taaaacatta aaccatgatg atttaccagc
      601 atttgtataa ccaactaaag ctacttgaaa cacctgattt tgatttcttt tatttcgata
      661 tctttcgcga tgttcttcta ccgtccgcaa ttgatgttta atttcattca tacgagttcg
      721 aatatgtctg cgatccatct ctaacttcgt ttcaccaggg cctctagttc caataccgcc
      781 acctaaacga gaaagacttt taccatggcc ttgcaatcta ggtaataaat aatcaagttg
      841 tgctagctct acttgcaatt taccttcttt acttcttgct cttaatgcaa atatttcaag
      901 aatcaactga gttctatcaa taatttttac acctaaagct tcatttagtg atttggattg
      961 tgcagtcgtt aattcatcat ttgtgatgac tacatcaata tctttgaact caataaatgc
     1021 ttgaatttct tcaattttac ctttaccaac ataatatttg cgatctacac gatctctgtt
     1081 ttgagtaatt tgacccaaca cttcaagttg gcaagtctct gataaagatg ataattcctc
     1141 cattgtagac tcaaaattaa attgcttatc atcttgagca tgtacaccga ctaagacagc
     1201 tttttctagt ttatttttag tatcatgaat ttgttgctga gccatgttat acatctcctt
     1261 aataaaatcc tactcaaagt attctatcat agagttttag taaagactat tacacgattt
     1321 tatggtacat tttaagtaaa tcgaaaggat gattcaatgg agacaattta tgattttgta
     1381 gtagaaacaa ataaaggtgt tacttacaaa ttagatgcat ataagggtga cgttatgcta
     1441 attgttaata cagctagcga atgtggcttt acttcacaat ttgaaggttt gcaatcatta
     1501 tacgagaaat acaaagatca agggtttgta attctaggtt tcccttgtaa tcaatttggt
     1561 ggtcaagaac ctggatcagg cgaagaagca gctcaaaact gtaaattaaa ctatggtgtt
     1621 accttcccta tgcatcaaaa aatagacgta aaaggggaac atcaattacc tttatttaga
     1681 tatttgactg cggcacaaca cggattcttt aatgagaaaa tcaaatggaa tttcactaag
     1741 tttttagtgg atcgcgaagg taacgttgtt aaacgatttg cacctc
//
LOCUS       DAOBBH010000281         1782 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.283, whole genome shotgun sequence.
ACCESSION   DAOBBH010000281 DAOBBH010000000
VERSION     DAOBBH010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.283"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            23..1684
                     /locus_tag="P6L01_002560"
     CDS             23..1684
                     /locus_tag="P6L01_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HDP3031595.1"
                     /translation="MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALAT
                     DARSYIYLVQDNEIDKVKEIVKQQNIDLLITKHDKKVFSSGKLKAIPNSDSYIQKRDV
                     FIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPI
                     QEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTT
                     IENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVE
                     KPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFV
                     ANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSH
                     IEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVIT
                     NLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARS
                     RDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE"
BASE COUNT          659 a          241 c          313 g          569 t
ORIGIN      
        1 tggaaagacc taaagaacaa tgatgaagtt tcaccaccgc ttaatgttat tgataagcag
       61 tatattaatt atcagttttg ttacattagg cattattatt cacgcttcca tcacatatgt
      121 tttatcagaa aaacacgaac aagctctagc aacagatgca agatcatata tttatttagt
      181 ccaagataat gagattgata aagttaaaga aattgtaaaa cagcaaaata tagatttatt
      241 gataactaaa catgataaaa aggtgtttag tagtggcaaa ctgaaagcta tcccaaatag
      301 tgatagctat attcaaaaaa gggacgtttt tatttttaat aagaaggtta acggttatca
      361 tgtatggatt aaaggttaca ataacgatat tacagaaatg cattggacgt tgtggaaata
      421 tcttatattg acatgtttag ttgtactcat ttgtctgtat tttgcaagcc gtagttttaa
      481 gcgtacatta atccgtccca ttcaagaagt tacttatgca acacaattgt tggcaaatgg
      541 ttattatcac atacgtgtac ctgaaagtaa tgttgtagaa acgaaagcgc tttttgtgtc
      601 taccaatgat ttagcacgac gccttcaaaa gcttaataat gagcaaaaaa ttcaatccaa
      661 ccgtttaaaa acaacgattg aaaatattcc aagtgctatt ttaatgatag atcgaaatgg
      721 aaaaatcgtt gttgcaaata aagcgtatta tgaacagttc aacatctcgc ataatattga
      781 acaagtaggc tatcacggct atgttaatac tgaaattgag caattaattt tagaaagttt
      841 taaagttgaa aaacctattt atgaacaatt ggaagttgca ataaaccaag tccatgccaa
      901 gtactttgat atttcatgcg ttccgatttt aactaggtct caaaaaagtt tgcaagggat
      961 tttagttgtg atgcatgata taacaaatct gaaacaactt gaaaatctaa gacgtgaatt
     1021 tgtagcaaat gtttcacatg aattaaaaac acctattact tcaattaagg ggtttgctga
     1081 aacgcttatt gatggtgcaa aaaatgatgc ggaatcatta gacatgtttt taaacataat
     1141 tttaaaagaa tcaaatcgaa tagaatcttt agtaacagat ctgttagatt tatcgcatat
     1201 tgagcaacat acagagttag atacagacta tatgaattta tcagatttaa cgcggcgtat
     1261 tattgataat atgatgactc aagccaatca aaaaaatatt tccattcata ctgatattga
     1321 aaaagatgtc attgttaaag cacaagaaag taaaattgct caagttatca cgaatttgtt
     1381 aacgaatgca attaactatt cttatgaaga tggagatatt aacgttcgtg tgtatcgaga
     1441 tgactttcgt gtcattttcg aagtacaaga ttttggtata ggtattaaat tagaagacca
     1501 acaacgtatt tttgaaagat tttatagagt agataaagca cgaagtagag attctggtgg
     1561 gacaggtctt ggattatcaa ttacaaagca tatagtagag gcccaccaag gcaatattga
     1621 agtgaatagt caagttggca aaggctcaac gttcaaagtt attctaaaag attataaaga
     1681 ataaaaatta aagtggtaac agcgcgtgta tttttcacga agttgttgct gcttttttat
     1741 ttcgttcaaa tctctattgg tattaaatta gagttaatga at
//
LOCUS       DAOBBH010000282         1767 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.284, whole genome shotgun sequence.
ACCESSION   DAOBBH010000282 DAOBBH010000000
VERSION     DAOBBH010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.284"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(55..627)
                     /locus_tag="P6L01_002561"
     CDS             complement(55..627)
                     /locus_tag="P6L01_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031596.1"
                     /translation="MEKNEYTAKYNEYSQLLDATYSQAVAYLLNKYGAVTDDYYKEKS
                     YTRFLNGEIKSISKGKYTRASEGLYCHHISEDKFQNLSDLRFISEFKYSYNVQKKENL
                     VYCDLIEHLILHAIITKESNGQFGVAGLCQMIKPTVIDWYISEYTPKPAWMQATKARA
                     YLPRILVEKLLIKIDDMLEGIEIYDFLESR"
     gene            complement(728..1069)
                     /locus_tag="P6L01_002562"
     CDS             complement(728..1069)
                     /locus_tag="P6L01_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HDP3031597.1"
                     /translation="MITFENIQQLEKYTLMTMHGLFNQLKLGIISIDNAEHTLFTPYM
                     MESLSSLGVKPDIIDLIHKGTELEDFAAFNLSIEDTVTVCLQRTEELLNQYKNVEFND
                     KILINWRIIQE"
     gene            complement(1110..1736)
                     /locus_tag="P6L01_002563"
     CDS             complement(1110..1736)
                     /locus_tag="P6L01_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031598.1"
                     /translation="MKFKAIVAITLSFSLLTACGANQHKENNSKSNDTTKKTQETDNT
                     TQSNTEKQMTPQEAEDIVRNDYKARGANENQTLNYKTNLERSNEHEYYVEHLVRDAVG
                     TPLKRCAIVNRHNGTIINIFDDMSEKDKEEFEAFKKRSPKYNPGMSDHDKTDGESEDI
                     QHHDNDNNKAIQNDTPDQKAVDKNDKNAVNKEEKHDNHAKNSAETKVK"
BASE COUNT          493 a          283 c          267 g          724 t
ORIGIN      
        1 tttaaacatc tataacaaaa tctcaacagc aaaaaataaa tctccattaa tcatttatct
       61 agactcaagg aaatcatata tttctattcc ttctaacata tcgtcaattt taatgagtag
      121 tttctctact aatattctag gcaaataggc acgtgctttg gtggcttgca tccatgctgg
      181 ttttggagta tattcgctaa tgtaccaatc aatgactgtt ggtttgatca tttgacataa
      241 tccagccaca ccaaattggc cattggattc ttttgtaata attgcatgta aaattaaatg
      301 ctcgattaga tcacagtaca ctaagttttc tttcttttga acgttgtatg agtacttaaa
      361 ttcggaaatg aatcttaggt cagataagtt ttggaatttg tcttcgctta tgtgatgaca
      421 atataatcct tcgctagctc tagtgtattt tccttttgaa atacttttga tttctccatt
      481 taaaaatcgc gtgtatgatt tttccttata ataatcatcg gttacagcgc catatttatt
      541 taaaaggtat gctacagctt gcgagtaagt agcgtctaat aattgactat attcattata
      601 tttagctgta tattcgtttt tctccacttc aatatacctc ttttcaaact ttatgatatg
      661 aaatgcttgt aatttacaaa gaataaaatc agtactcacg tagttacatt cgacttttat
      721 atgtctatta ctcttgtata atacgccaat tgattaatat tttgtcattg aattccacat
      781 ttttgtattg atttaatagt tcttcagttc tttgtaaaca gactgtaact gtatcttcaa
      841 ttgataaatt aaatgctgca aagtcttcta actctgtccc tttgtgaatt aaatcgataa
      901 tgtctggttt cacacctagg gaagagagtg attccatcat ataaggtgta aagagggtat
      961 gctctgcatt gtcgattgaa ataataccga gtttaagttg attaaaaaga ccatgcatag
     1021 tcattaaggt gtatttttca agttgttgta tattctcaaa tgttatcata gtttttctcc
     1081 taaaaattac aattaatcaa agtataccct tatttaactt tagtttctgc tgaatttttt
     1141 gcatggttat catgtttttc ttctttatta acagcatttt tatcgttttt atcaacggct
     1201 ttttgatctg gtgtatcatt ttgaatggct ttgttattat cattgtcatg atgttgaatg
     1261 tcttctgact caccatctgt tttatcatga tcactcatac ctgggttgta tttagggctt
     1321 ctctttttaa atgcttcaaa ttcttcttta tctttttctg acatatcatc aaaaatatta
     1381 ataatcgtac cattgtgtcg attaacaata gcgcaacgtt ttaaaggtgt gccaactgca
     1441 tcgcggacta gatgttcaac ataatattca tgttcattac tccgttcaag atttgtttta
     1501 taatttaaag tttgattttc gttagcacct cttgctttgt aatcgtttcg aacgatatct
     1561 tcggcttctt gtggtgtcat ttgcttttct gtatttgact gtgtagtgtt gtcagtttct
     1621 tgcgtctttt tagtagtgtc atttgattta ctattatttt ctttatgttg attagcaccg
     1681 caggcagtta atagtgaaaa tgataatgtg attgcaacga tagctttgaa tttcattttt
     1741 taatctcctt atattgctat gtatatt
//
LOCUS       DAOBBH010000283         1759 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.285, whole genome shotgun sequence.
ACCESSION   DAOBBH010000283 DAOBBH010000000
VERSION     DAOBBH010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.285"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..183
                     /locus_tag="P6L01_002564"
     CDS             <1..183
                     /locus_tag="P6L01_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDP3031599.1"
                     /translation="FTREGKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVIPEMEYE
                     MEEVKQIEQYIKEQEE"
     gene            386..1069
                     /locus_tag="P6L01_002565"
     CDS             386..1069
                     /locus_tag="P6L01_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HDP3031600.1"
                     /translation="MTTKENIDTLRKPGAQALSLISLFLILFSCLTFFFGLDYERFPN
                     YLKITTIIELIIIVISLLQWIRFIDFEKESARKYKKIYARFLVIINVLTTITVVFALC
                     NLYYFAAVQNHYDLFNYWLMGTISIIISYLLLVIGGMFTLLKLPKVTKRWGGKTKTHF
                     GLLLTALSSFIYIEKIIEYILIPNVVESKFIIIVSMLVIAGAQFVAFQFIMQYSRFYI
                     FELNTEDDD"
     gene            1215..>1759
                     /locus_tag="P6L01_002566"
     CDS             1215..>1759
                     /locus_tag="P6L01_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HDP3031601.1"
                     /translation="MEFLLLIVVAGLYYIIYLTAVMYSEKIVVLPIIIYAILFVIIGI
                     TYIFIGDSYDQLTNFNVILYMGSLFYAWMAIRNLWNRPLLLKYKNITDSSSGIVNKSE
                     YNSVESLRINIEIAKYKGIISLIVAIVLTVLMTLKSTPQITAETRDLSISFFILSLFI
                     IIIFAVWDLFIRVRKGAFAFVV"
BASE COUNT          629 a          193 c          297 g          640 t
ORIGIN      
        1 tttacaagag agggtaaatt gaaagtatca tttgattata tagattggat aaatacagag
       61 tttgatcaat tgggccgtga aaattattat atgtacaaaa aatttggggt tataccagaa
      121 atggaatatg aaatggagga ggttaaacaa atcgagcaat atattaaaga gcaagaagaa
      181 tagacaatct taatgtgaga ctaaacaata aagctttgtt tagtcttttt tggcggttaa
      241 gtaaaaagca atagataccg taaagttgat gctcgtcaaa taataatata aagatagttt
      301 tatgctttgt atagataaaa acctaagtac ttataacttt aaatacgtaa tatgctattt
      361 aaaagataat gtataggaga cagatatgac tactaaagaa aatattgata ctcttcgaaa
      421 gccaggtgca caagccttaa gtttaatatc attgtttttg atactttttt catgtctaac
      481 tttctttttt ggtttagatt atgaaaggtt tccaaactat ttaaagataa cgacaattat
      541 agaattaata attattgtaa ttagtttact tcaatggatt aggtttatag atttcgaaaa
      601 agaaagtgca cggaaatata aaaaaatata tgctcgattt ttagttatta taaatgtgtt
      661 aactactatc actgtagtat ttgcactgtg taacctttat tattttgcag ctgtacaaaa
      721 ccattatgat ttattcaatt attggttaat gggtacgatt tcaatcataa ttagctattt
      781 attattagta attggcggaa tgtttacgtt gttaaaatta cctaaagtaa caaaacgctg
      841 gggtggtaaa actaaaacac atttcggttt attattaact gcgttgagct catttatata
      901 tattgaaaaa attatcgaat atatattgat tcctaatgtc gtagaatcca agtttataat
      961 tattgtgagc atgttggtta tcgctggagc acaatttgtg gcatttcaat ttattatgca
     1021 atacagtaga ttctatattt ttgaattaaa cacagaagat gatgactaaa tgattttgta
     1081 aatactgtga agtatactta aattaagaaa atcaacttca acgtatatca ccatattatt
     1141 ttatactcaa tgatgtgaat ggatttaagt ggattgatat taaagtaaaa gttatggaat
     1201 attgaggtgc gaatatggag ttcttattat taattgtcgt agccggactg tattatatta
     1261 tatatttaac tgctgtgatg tattctgaaa aaatagtagt attgcctata atcatctatg
     1321 ccattttgtt tgtaataatt ggtatcactt atatctttat aggcgacagc tatgatcaat
     1381 taacaaattt caatgtgatt ttgtatatgg ggagtttgtt ttatgcatgg atggctatta
     1441 gaaatctttg gaacagacca ctattactaa aatataagaa cattacagat agttcaagtg
     1501 gaatagttaa taaatctgaa tacaattcag ttgaaagctt acgtataaat attgaaatag
     1561 ctaagtataa agggattatt tctttgatag tagctatagt actaacggta ttaatgacat
     1621 taaagtcaac acctcaaatc actgcagaaa cacgtgactt aagtatctca tttttcatac
     1681 tcagcttatt tatcattatt atatttgcag tttgggattt gtttattaga gttagaaaag
     1741 gagcgtttgc ttttgttgt
//
LOCUS       DAOBBH010000284         1759 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.286, whole genome shotgun sequence.
ACCESSION   DAOBBH010000284 DAOBBH010000000
VERSION     DAOBBH010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.286"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..891
                     /locus_tag="P6L01_002567"
     CDS             <1..891
                     /locus_tag="P6L01_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031602.1"
                     /translation="FVNISFVIITIALFIALYYFNKIGFWYRKEDIYLSSTNINFSLP
                     ISIKAITTIQLKHWISNKEEVNLHKPFFYISYSIWFFLGCLIYFSQMAYNQLANAVIF
                     ILILNTITRDSFSAGTDFFTKSLRFDSDRKSIGLYRMSNTDFKQIYDSKLSLIRFIGF
                     KETILAIILLAIFLNQDIYLYIIGFEIIIINTVIIPNLSLLPSYLSPHFNHQHYSELE
                     SFEEQIFLEDTVFDKVKNFLSSSYFLILFVGYLSQRNYIDIMIFIIIFNILALILVSL
                     FIRFSKQKITKSWRKRDLYL"
     gene            929..1393
                     /locus_tag="P6L01_002568"
     CDS             929..1393
                     /locus_tag="P6L01_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001793228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HDP3031603.1"
                     /translation="MLIIGGLLGCLVYYLLNIDLGKMIVAQDLNLQEIFFHNLIYFFS
                     AIFGFLTLGLLNIGMLLINSGMLGFFIAHGIYSNQLKSILLVLAPHAIFEILALLIAS
                     TFSLGFLSYLYQRMVKKNKIKNKMFHNFVTTIITVIVLTIIASLLEVYVTIN"
     gene            1405..>1759
                     /locus_tag="P6L01_002569"
     CDS             1405..>1759
                     /locus_tag="P6L01_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDP3031604.1"
                     /translation="MLVLNNLQKKFTNSKMVLNNVNAKFRSGSINCIVGVNGAGKTTL
                     LNIISSILMPTKGDVYLNSESIFENSTLKKEIFYTPVNPFFYENLSAKDNLYLICSLY
                     NRKIDQITIEKTIKDV"
BASE COUNT          634 a          194 c          238 g          693 t
ORIGIN      
        1 tttgtgaata tatcatttgt gattattaca atagctttat ttatagcgtt atattatttt
       61 aataaaatag ggttttggta tagaaaagaa gatatctatc tatcttcaac aaatattaat
      121 ttttcgttgc caatttcaat aaaagctata acaacaatac agctaaaaca ctggataagt
      181 aacaaagaag aagttaattt acataaacct ttcttctata ttagttattc aatatggttc
      241 tttttagggt gtttaatcta tttttcacag atggcatata atcagttagc taatgcagtt
      301 atttttattt tgatattaaa cactataact cgtgatagtt tttctgcggg gacggatttt
      361 tttactaaat cattacgttt tgattcagat agaaaaagta tcggtttgta taggatgtca
      421 aacactgatt ttaaacaaat ttacgattct aaattatctc taattcgctt tattggtttt
      481 aaagaaacaa tacttgctat tattttatta gcaatatttt taaatcaaga catatattta
      541 tatataattg gatttgaaat aataatcatc aatacagtaa taattcctaa tttatcatta
      601 ttaccttcgt atttatctcc acatttcaat catcaacatt acagtgaatt agagtctttt
      661 gaagaacaaa tttttcttga agatactgtt tttgataaag ttaagaattt tttatcgtca
      721 tcatattttt taattctatt tgtaggttac ttatctcaac gtaattatat tgacataatg
      781 atttttataa ttattttcaa cattttagcc ttaatacttg tttcgttgtt tattaggttt
      841 tctaaacaaa aaataacaaa gtcttggaga aagagggatt tatatttata gtattaaata
      901 caaaaaaaat tttattttta atatattggt gttaataata ggcggattat tgggatgctt
      961 ggtttattac ttattaaata ttgatttagg taaaatgata gttgctcaag atttaaattt
     1021 acaagagatt ttctttcata atttaattta tttcttctct gcaatatttg gttttctgac
     1081 acttggtctg ttaaatatag gtatgttact cataaattct gggatgttag gcttttttat
     1141 agcacatgga atctatagca atcaattaaa atctattcta ttagtattag cgccacacgc
     1201 tatatttgag attttagcac ttttaattgc ttcaacattc tcattaggtt ttttatcata
     1261 cctttatcaa agaatggtaa agaaaaataa aataaaaaat aagatgttcc ataattttgt
     1321 aacaacaata ataactgtta ttgtattaac aataatcgca tcattattag aagtttatgt
     1381 aaccataaat taaggaagtg tatcatgcta gttttaaata atcttcaaaa aaaatttact
     1441 aattcaaaaa tggttttgaa taatgtgaat gctaagttta gaagtgggag catcaattgc
     1501 atagtgggag taaatggggc aggtaaaacg actttgttaa atattattag tagtatattg
     1561 atgccaacaa aaggtgatgt atatttaaat agtgaaagta tttttgaaaa tagcacacta
     1621 aaaaaagaga ttttttacac accagtaaat cccttttttt atgaaaattt aagtgctaaa
     1681 gataatttgt acttaatatg tagcctatat aatcggaaaa tagatcaaat aactattgag
     1741 aaaactatta aagatgtag
//
LOCUS       DAOBBH010000285         1748 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.287, whole genome shotgun sequence.
ACCESSION   DAOBBH010000285 DAOBBH010000000
VERSION     DAOBBH010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.287"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..1716
                     /gene="infB"
                     /locus_tag="P6L01_002570"
     CDS             <1..1716
                     /gene="infB"
                     /locus_tag="P6L01_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDP3031605.1"
                     /translation="GEFADKLNVESSEIIKKLFLLGIVANINQSLNQETIELIADDYG
                     VEVEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHVDHGKTTLLDSIRHTKVTA
                     GEAGGITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARGAQVTDITILVVAADDGVM
                     PQTIEAINHAKEAEVPIIVAVNKIDKPTSNPDRVMQELTEYGLIPEDWGGETIFVPLS
                     ALSGDGIDDLLEMIGLVAEVQELKANPKNRAVGTVIEAELDKSRGPSASLLVQNGTLN
                     VGDAIVVGNTYGRIRAMVNDLGQRIKTAGPSTPVEITGINDVPQAGDRFVVFSDEKQA
                     RRIGESRHEASIVQQRQESKNVSLDNLFEQMKQGEMKDLNVIIKGDVQGSVEALAASL
                     MKIDVEGVNVRIIHTAVGAINESDVTLANASNGIIIGFNVRPDSGAKRAAEAENVDMR
                     LHRVIYNVIEEIESAMKGLLDPEFEEQVIGQAEVRQTFKVSKVGTIAGCYVTEGKITR
                     NAGVRIIRDGIVQYEGELDTLKRFKDDAKEVAKGYECGITIENYNDLKEGDVIEAFEM
                     VEIKR"
BASE COUNT          613 a          249 c          371 g          515 t
ORIGIN      
        1 ggcgaatttg cggataaatt aaatgttgaa tcatcagaaa ttatcaaaaa attattctta
       61 cttggtattg ttgctaatat caatcaatca ttaaatcaag aaacaatcga attaattgcc
      121 gatgattatg gcgttgaggt tgaagaagaa gttgtgatta atgaagaaga tttatcaatc
      181 tacttcgaag acgaaaaaga tgatccagaa gcaattgaga gaccagcagt tgtaacaatt
      241 atgggacatg ttgaccatgg taaaacgact ttattagatt caattcgtca tacaaaagtt
      301 acagcaggtg aagcaggcgg aatcactcaa catattggtg catatcaaat tgaaaacgat
      361 ggcaaaaaaa tcactttctt agatacaccg ggacatgctg catttacaac gatgcgtgcg
      421 cgtggtgcac aagtaacaga tattacaatt ttagtagtag cagctgacga tggtgttatg
      481 ccacaaacaa ttgaagcaat taaccatgct aaagaagcag aagtaccaat tattgttgca
      541 gtaaataaaa ttgataaacc aacttcaaat cctgatcgag ttatgcaaga attaactgaa
      601 tatggtttaa ttcctgaaga ttggggcggc gaaacaattt tcgttccact ttctgcatta
      661 agtggtgatg gtatcgacga tttattagaa atgataggat tagttgcaga agttcaagaa
      721 cttaaagcaa atcctaaaaa ccgtgctgtt ggtacagtta tcgaagctga attagataaa
      781 tcacgtggtc cttctgcatc attattagta caaaacggta cattaaatgt tggtgatgcg
      841 attgtagttg gtaatactta cggccgtatc cgtgcaatgg ttaatgactt aggtcaaaga
      901 atcaaaacgg ctggtccatc aacgcctgtt gaaattacag gtattaatga tgtgccacaa
      961 gctggggatc gctttgttgt atttagtgat gaaaaacaag ctcgtcgtat tggtgaatca
     1021 agacacgaag ctagcattgt acaacaacgt caagaaagta aaaatgtttc attagataac
     1081 ctgtttgaac aaatgaaaca aggtgaaatg aaagatttaa acgttattat taaaggtgat
     1141 gttcaaggtt ctgttgaagc tttagctgca tcattaatga aaattgatgt tgaaggcgta
     1201 aatgttcgta tcattcatac agcggttggt gcaattaatg agtcagacgt gacacttgct
     1261 aatgcctcaa atggtattat cattggtttc aatgttcgtc cagacagtgg tgcaaaacgt
     1321 gctgcagaag ctgaaaatgt tgatatgcgt ttacacagag ttatttataa tgttatcgaa
     1381 gaaattgaat cagcgatgaa aggtttactt gatccagaat ttgaagaaca agttatcgga
     1441 caagctgaag ttcgtcaaac attcaaagtt tctaaagttg gtactattgc tggatgttat
     1501 gttactgaag gtaaaattac gcgaaatgct ggtgtacgta ttattcgtga tggtattgtt
     1561 caatatgaag gcgaattaga tacacttaaa cgtttcaaag atgatgctaa ggaagttgca
     1621 aaaggttatg aatgtggtat tacaattgaa aactacaatg accttaaaga aggcgatgtt
     1681 atcgaagcat ttgaaatggt tgaaattaag cgttaattaa ataaattaca agctaaaagt
     1741 atagttaa
//
LOCUS       DAOBBH010000286         1705 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.288, whole genome shotgun sequence.
ACCESSION   DAOBBH010000286 DAOBBH010000000
VERSION     DAOBBH010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.288"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(148..351)
                     /locus_tag="P6L01_002571"
     CDS             complement(148..351)
                     /locus_tag="P6L01_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031606.1"
                     /translation="MLHETWKERTPIKKVEVINTDAKKFTVSDMLTVGKQYDVINETE
                     EYYQIIDNSGLVGGYYKTYFKEV"
     gene            complement(380..1189)
                     /locus_tag="P6L01_002572"
     CDS             complement(380..1189)
                     /locus_tag="P6L01_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDP3031607.1"
                     /translation="MCGLRSITLGTTNIEQTKHFMVDILGLNYEELLENSIRFGDADI
                     SPGTRLQFIQVPSEQLEESHFVGIGLRTPTDSGLEEYAEILSNKDIPFTTVKELNGNK
                     YFSLEDNNGHIFSIYSNENNYGVGLGMPSFESAVNPLHQVQGLGPVILKVNHVDITGQ
                     ILTNIFGLEVFAEYQPFDNADYHVQVFKVGTGGLGGEIHLMPVETEMTMPEYGAVDQV
                     EFETKDADFFNQAKSRLDEVEIPYQTLEQDDIESIRITENSGLSFIFTLQK"
BASE COUNT          547 a          284 c          226 g          648 t
ORIGIN      
        1 ctaagacatg gaataagttc ttcaaatgcc ttagtttact attctattct gatataaaac
       61 gcaatactta gtatgcgaat tgtttatatt ttaccaaatt tactaattga aaatctcata
      121 ttaggcttca tttcaataat tatgcgttta aacttctttg aaatatgttt tatagtaacc
      181 gccaactaat ccagaattat ctattatttg ataatattct tcagtttcgt tgataacatc
      241 atattgtttg ccaaccgtta acatgtcaga aactgtaaat ttctttgcat ctgtattaat
      301 gacttctact ttcttgattg gtgtacgttc tttccaagtt tcatgtaaca taaattttgt
      361 cctcactatc gtaaaaaaat tatttttgta aagtgaatat aaacgataat ccactgtttt
      421 cagtaattct aattgattca atatcatctt gctcaagcgt ttgatatggt atttccactt
      481 catctaagcg cgatttcgct tgattaaaga aatctgcatc tttcgtttca aactcaactt
      541 gatcgactgc gccatattcc ggcattgtca tttctgtttc tacaggcatt aaatgtattt
      601 cgccacctag accacccgtt ccaactttga atacttggac atgatagtca gcattgtcga
      661 agggttggta ttctgcaaat acttcaagtc cgaatatatt tgttaaaatt tgacctgtaa
      721 tatctacatg attcacttta agaatcactg gtcctaaacc ttgcacttga tgtaacggat
      781 tgaccgcact ctcaaaagaa ggcataccta aaccaacgcc ataattattc tcgtttgaat
      841 atattgagaa aatatgacca ttgttatctt cgagactgaa atatttattg ccatttaatt
      901 ctttaactgt tgtaaatgga atatccttat tcgataatat ttccgcatac tcctctaaac
      961 ctgagtcagt tggtgtacgt aatccaatac ccacaaagtg agattcttct aattgctcac
     1021 ttggaacttg tataaattga agtcttgttc ctgggcttat atctgcatcg ccgaaacgaa
     1081 ttgagttttc aagaagttct tcataattta atcctaatat gtcaaccatg aaatgttttg
     1141 tctgttctat atttgttgta cctaatgtta tacttctaag tccacacatt atagccacct
     1201 ctatctttat ctttactatt atattataac ttattttaat cataaattat agaatatcgg
     1261 tcagttgatt ttgattatag attaatatgt gaaaaatgta tttctttaaa agaactattt
     1321 catttaaata ggtaacataa tattgttatg gttgctattg agtatgtcat taatgagtat
     1381 ctgtatatag caattgaata tttcgtttta tgtagtacat aaaaaacata tacatgtcgt
     1441 atccataaag tgaatttaca tcttgttcag atgattttaa attaactaaa ggcgcttata
     1501 gaattcataa atttcatcgc ttgttattat ctaaaatgat ttcattagca tattgaaaca
     1561 cgccattcca cctatatttc tattgtgagt ctatttcaat aaaaatgata tattttttaa
     1621 gttgtcaaaa ttaaactcac attttaatac gaagcattat aaaaataaag tacagcagta
     1681 agatatttcg actagaaaat aatct
//
LOCUS       DAOBBH010000287         1702 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.289, whole genome shotgun sequence.
ACCESSION   DAOBBH010000287 DAOBBH010000000
VERSION     DAOBBH010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.289"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            371..1096
                     /locus_tag="P6L01_002573"
     CDS             371..1096
                     /locus_tag="P6L01_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL14"
                     /protein_id="HDP3031608.1"
                     /translation="MKKNIMNKLVLSTALLLLGTTSTQLPKTPISFSSEAKAYNISEN
                     ETNINELIKYYTQPHFSLSGKWLWQKPNGSIHATLQTWVWYSHIQVFGSESWGNINQL
                     RNKYVDIFGTKDEDTVEGYWTYDETFTGGVTPAATSSDKPYRLFLKYSDKQQTIIGGH
                     EFYKGNKPVLTLKELDFRIRQTLIKNKKLYNGEFNKGQIKITADGNNYTIDLSKKLKL
                     TDTNRYVKNPKNAQIEVILEKSN"
     gene            1191..>1702
                     /locus_tag="P6L01_002574"
     CDS             1191..>1702
                     /locus_tag="P6L01_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HDP3031609.1"
                     /translation="MNNNITKKIILSTTLLLLGTAFTQFPNTPINSSSEAKAYYINQN
                     ETNVNELTKYYSQKYLTFSNSTLWQKDNGTIHATLLQFSWYSHIQVYGPESWGNINQL
                     RNKSVDIFGIKDQETIDSFALSQETFTGGVTPAATSNDKHYKLNVTYKDKAETFTGGF
                     PVYEGNKPVLT"
BASE COUNT          663 a          253 c          240 g          546 t
ORIGIN      
        1 tattaaaatt tgtaatattt tatcaataat taaatgaaca ttttattaat attaaattta
       61 agtagtagga aataattaaa ataagtacta catttaaagt ataactattt ttcaagtagt
      121 tagaaaattc aattatcaaa caatttaatg caattgatta gagaataatt gtaacgtgtc
      181 gtttttaata tataactccc gcctacttta ttaagtactg tttctgtcca aaacttaaaa
      241 atgataagtt ttgcttaaat aacactacta actgtttaag tttatttaac atagttttag
      301 cttttattta attccgaatc ggtgtaatag cttatatact ttgggtaatt cacgcaaagg
      361 agattttcat atgaaaaaga acatcatgaa taaattagtt ttatcaacag cattgttact
      421 tttaggaacc acatcaacac aacttcctaa aacaccaatc agtttttcat ctgaagcaaa
      481 agcctataat atcagtgaaa acgagactaa tatcaatgag ttaatcaaat attacactca
      541 gccgcatttt tcattatctg gaaaatggct atggcaaaag cccaatggta gcattcatgc
      601 aacattgcaa acgtgggttt ggtatagtca tattcaagtg tttggatccg agagttgggg
      661 aaacattaat cagttaagaa ataaatacgt tgatatattt ggaactaaag atgaggacac
      721 agttgaaggt tactggactt atgatgaaac atttactggt ggtgttacgc cagcagctac
      781 ttcatctgat aaaccttata gactattttt aaaatatagt gataaacaac aaactatcat
      841 cggtggacat gaattttaca aaggaaataa accagtatta actttaaaag aattagattt
      901 ccgtattcgt caaacattaa taaagaataa aaagttatat aacggagaat ttaataaagg
      961 tcaaattaag ataactgctg atggaaataa ttacacgatt gatttaagta aaaagttaaa
     1021 attaactgac acaaaccgtt atgttaaaaa tcctaaaaat gcacaaattg aagtcatact
     1081 cgaaaaatct aactaaccta ttaccttttg taaatgcgga taatttcaat tatctaatta
     1141 accccttttt ataattaaac attccaacaa tactcaaagg agaaattcga atgaacaata
     1201 acatcacgaa aaaaattatt ttatcaacaa cattgttact attaggtaca gcatttacac
     1261 aatttcctaa tacacctatc aattcttcat ctgaagcgaa agcttattat ataaatcaaa
     1321 acgaaactaa cgttaatgag ttaactaaat attactcgca aaaatattta accttctcta
     1381 acagtacgtt atggcaaaaa gataacggta cgattcatgc aacgttgtta cagttttctt
     1441 ggtatagtca tattcaagtt tatggacctg aaagttgggg caatatcaac caattaagaa
     1501 ataaaagcgt tgatattttt ggcataaaag accaagaaac cattgattct tttgcattat
     1561 ctcaagaaac gtttactggt ggtgttactc ctgcagcaac atctaacgat aaacactata
     1621 aactgaatgt aacatataaa gataaagcag aaacgtttac tggcggattt ccagtttatg
     1681 aaggcaataa gcctgtttta ac
//
LOCUS       DAOBBH010000288         1683 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.290, whole genome shotgun sequence.
ACCESSION   DAOBBH010000288 DAOBBH010000000
VERSION     DAOBBH010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.290"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(181..1167)
                     /locus_tag="P6L01_002575"
     CDS             complement(181..1167)
                     /locus_tag="P6L01_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDP3031610.1"
                     /translation="MNDQWYKHLIGARTIKTGIAIFLTAVFCMALDLTPIYAILTAVV
                     TIEPTAKASLIKGYRRLPATVIGAGFAVLFTYLFGDQSPFTYALSATFTILFCTKLKL
                     QVGTNVAVLTSLAMIPGIHDAYIFNFLSRTLTAIIGLVTSGLINFMVFPPKYYGQVEE
                     KLSKTDALMYKLFYNRCQELILSRLQSDKSEKAYKNIFNLNNQVETLISYQRDELSYH
                     KKKECDWKLLNQLTKRAYTNRLFITHLSNIIYLPKNTRVNFSGDEKMALLKISSSIKD
                     IFYDGTFKREDDSVETLRSTIKALEISGENQIKSHILYEVLMIYRLLDSRYA"
BASE COUNT          567 a          278 c          287 g          551 t
ORIGIN      
        1 agagaaattg gattcccaat ttctacagac aatgcaagtt ggggtgtggg tcccaacaca
       61 gagaatttcg aaaagaaatt ctacaggcaa tgcgagttgg ggtgggacga cgaaataaat
      121 tttacgaaaa tatcatttct gtcccactcc catggtgcca attagcataa ggtacttaaa
      181 ttaagcatat ctgctgtcta gcagtcgata aatcattaga acttcgtata gtatatgact
      241 tttaatttga ttttcaccac taatttcaag tgcttttata gtcgaacgta aagtttctac
      301 agaatcatct tctctcttaa aagtaccatc ataaaatata tctttgatgc tactactaat
      361 ttttagcaat gccatttttt cgtcacctga aaagttaaca cgagtatttt taggcaagta
      421 aatgatattt gataaatgag tgataaacaa acgattcgta tatgcacgtt tagttaattg
      481 attgagtaat ttccaatcac attctttttt cttatgatag cttaattcat cacgttgata
      541 acttattaac gtttcaactt gattgtttaa attgaaaata tttttatatg ctttttcgct
      601 tttatcagat tgcagtcttg ataagataag ttcttggcag cgattgtaaa ataatttata
      661 catcaaggca tctgtcttac ttaatttttc ttcgacctga ccataatact taggtggaaa
      721 caccatgaag ttaattaaac ctgatgtcac gagtccaata attgctgtca atgttcgaga
      781 caaaaagttg aatatgtagg catcatgaat acctggaatc atagctaatg atgttagtac
      841 agcgacattc gtaccaactt gcaatttgag ttttgtacag aataaaatcg tgaacgttgc
      901 actcaatgca tatgtaaaag gtgattgatc gccgaataaa tatgtaaata atactgcaaa
      961 gcctgcacca attaccgtag caggtaatct acgataacct ttaataagtg atgccttggc
     1021 agttggttca attgtgacta cagctgttaa aatggcatag atgggtgtta aatctagtgc
     1081 catacaaaag acagctgtta aaaaaatggc aataccagtt ttaattgttc tggcaccaat
     1141 taaatgttta taccattgat cgttcatttt ttaacctcta atcatcgtaa aatcttagcg
     1201 agcgctttat aataatagta tcgtacattg gaaaagttca tgtatgtaaa atatttgaaa
     1261 taatcatact taagcattac tttgattttc atatacatta atcaaagttc tgggctccac
     1321 aatgttatat tgttagtagt tgactgaaat aaaatgcact tgtaacaagc atttttcaat
     1381 tatagtccgg ggccccaaca cagaagctga cgaaaagtca gcttacaata atgtgcaagt
     1441 tggggtgtgg gccccaacac agaagctgac gaaaagtcag cttacgataa tgtgcaggtt
     1501 ggcggggccc caacatagag aaattggatc tacaatttct acaggcaatg caagttgggg
     1561 tacaacgata aagaaatatt ttttctttat cacactatgt ctcactcact ttccaaaata
     1621 ctaaagtaac atctttagta tatcaaagaa tttttgctat aataagttat aattatataa
     1681 aaa
//
LOCUS       DAOBBH010000289         1682 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.291, whole genome shotgun sequence.
ACCESSION   DAOBBH010000289 DAOBBH010000000
VERSION     DAOBBH010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.291"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            126..986
                     /locus_tag="P6L01_002576"
     CDS             126..986
                     /locus_tag="P6L01_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDP3031611.1"
                     /translation="MAKELCFEGITLKAFDEQYRSAINDFDLNERQQIYSSLPKEVID
                     DAINDVDRIANVAINDKNEVVGFFVLHRYYQHEGYDTPENVVYIRSLSINEKYQGFGY
                     GTKIMMSLPQYVQGVFPDFNHLYLVVDAENDNAWNLYERAGFMHTATKEEGPIGKERL
                     YYLDLDSKHVSSLKLEEESRSEVTNVHIINLMIDGQKVGFIALEQIGERMNIAAIEVD
                     KSYRFNGIGSSALRQLPTYLRKNYDNLNVITMILFGENNDFKPLCLNSNFVEIEQTDD
                     YVVFEKYLNY"
     gene            1098..1628
                     /locus_tag="P6L01_002577"
     CDS             1098..1628
                     /locus_tag="P6L01_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDP3031612.1"
                     /translation="MKIQDYTKQMVDEKSFIDMAYTLLNDKGETMNLYDIIDEFRALG
                     DYEYEEIENRVVQFYTDLNTDGRFLNVGENLWGLRDWYSVDDIEEKIAPTIQKFDILD
                     ADDEEDQNLKLLGEDEMDDDDDIPAQTDDQEELNDPEDEQVEEEINHSDIVIEEDEDE
                     LDEDEEVFEDEEDFND"
BASE COUNT          625 a          193 c          329 g          535 t
ORIGIN      
        1 aaatttaaaa aaatataaat aagtaattta tttaacttag agtaaataat ggtatcgtag
       61 tgaaataata ggtaaaataa tatggggatt catgcttcat atataaaaag ataggggtta
      121 aatatatggc taaagaactt tgttttgaag gtatcacttt aaaagcattt gatgaacaat
      181 atcgttcagc aattaatgat tttgacttga atgaaagaca acaaatatat tcatctttac
      241 ctaaagaagt tattgatgat gcaattaatg atgttgatag gattgctaac gtagcaataa
      301 acgataaaaa tgaagtggtg ggcttttttg tattacatcg ttactatcag catgaaggtt
      361 atgatacacc tgaaaatgtc gtttatattc gttcattatc gattaatgaa aaatatcaag
      421 gttttggata tggcacgaaa ataatgatgt cattgccgca atatgttcaa ggtgtatttc
      481 ctgattttaa tcatctatat ctagtagtag atgcggaaaa tgacaatgct tggaacctat
      541 acgaacgtgc tggattcatg catacagcga ctaaagaaga aggaccaatt ggcaaagaac
      601 gactatatta cttggactta gattcaaaac atgtttcgtc attaaagctt gaagaagaaa
      661 gtcgttcaga agtgaccaat gtacatatca ttaatttaat gattgatggc caaaaggttg
      721 gctttatcgc attggagcag attggtgaac gcatgaacat tgctgctatt gaagtggata
      781 aatcatatcg ctttaatggt attggttcaa gtgcgctgcg acaattgcca acttacttaa
      841 gaaaaaacta tgacaacctt aatgtgatta cgatgattct gtttggagag aataatgatt
      901 ttaaaccatt atgtttaaat agtaatttcg ttgaaatcga acaaactgat gattatgtcg
      961 tttttgaaaa atatttaaat tactaacagt gattgcgaaa tatgatattg tcatttataa
     1021 tttagttttg ttactatata taaatgaatt cagacgtata aatttagatt atatccttcg
     1081 aaaggaagta ttgggcaatg aaaattcaag attatacaaa acaaatggtt gatgaaaaat
     1141 catttattga tatggcttat acattattga atgataaagg cgaaactatg aacttatatg
     1201 atatcatcga tgagtttaga gcgttaggtg attatgagta cgaagaaatt gaaaatcgcg
     1261 ttgtacaatt ttacacagat ttaaacacag atggtcgttt tttaaatgtt ggagaaaatt
     1321 tatggggatt acgtgattgg tattcggtag atgatattga agagaaaatc gcaccaacta
     1381 ttcaaaaatt cgatattctg gatgcagatg atgaagaaga tcaaaactta aaattattgg
     1441 gcgaagatga aatggatgac gacgatgata ttccagctca aacagatgat caagaagaac
     1501 taaatgatcc agaagatgag caggttgaag aagaaatcaa tcattcggat atagtcattg
     1561 aagaagatga agatgaacta gacgaagacg aagaagtgtt tgaagacgaa gaagacttca
     1621 acgattaatt ttttgtttga cttttagttg aaagatgata aaattttatt cgggctcctt
     1681 ta
//
LOCUS       DAOBBH010000290         1671 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.292, whole genome shotgun sequence.
ACCESSION   DAOBBH010000290 DAOBBH010000000
VERSION     DAOBBH010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.292"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..1205
                     /locus_tag="P6L01_002578"
     CDS             <1..1205
                     /locus_tag="P6L01_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDP3031613.1"
                     /translation="EKIQLLADIVELQTENNNEIDVCNYLKDLFDKYDIKSEILKVNE
                     HRANIVAEIGNGSPILALSGHMDVVDAGNQDNWTYPPFQLTEKAGKLYGRGTTDMKGG
                     LMALVITLIELKEQNQLPQGTIRLLATAGEEKEQEGAKLLADKGYLDDVDGLIIAEPT
                     GSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNQFKEKYSELKKHDTK
                     HELDVAPMFKSLIGKEISEEDANYASGLTAVCSIINGGKQFNSVPDEASLEFNVRPVP
                     EYDNDFIESFFQNIINDVDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVASSYVEQDE
                     IFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKYIDIFKEA
                     SIQYLKEK"
BASE COUNT          580 a          267 c          302 g          522 t
ORIGIN      
        1 aagaaaaaat tcaattacta gcagatattg ttgaactaca aactgaaaat aataatgaaa
       61 tagacgtttg taattattta aaagatttat tcgacaagta cgatattaaa tctgaaattt
      121 tgaaagttaa tgaacaccgc gccaatatcg ttgcagaaat cggtaacggc tcacctatac
      181 tcgcattgag tggtcatatg gatgttgttg atgcaggaaa tcaagataat tggacttatc
      241 ccccttttca actcacagaa aaagctggca aattatacgg tcgaggcaca acggatatga
      301 aaggcggtct gatggctttg gtcatcacac tcattgaatt aaaagaacaa aatcaattgc
      361 ctcaaggaac gataagatta ctagctactg ctggcgaaga gaaagaacaa gaaggtgcca
      421 aattattggc tgataaaggc tatttagacg atgtagatgg cttaattatt gctgaaccaa
      481 ctggatctgg aatttattat gcacataaag ggtctatgtc atgtaaagta actgcaactg
      541 gcaaagctgt ccatagctca gttccattta ttggtgacaa tgcaattgat acactgcttg
      601 aattttataa tcaatttaaa gaaaaatatt cagagcttaa aaaacatgat actaaacatg
      661 aattagatgt tgcacccatg ttcaaatcat tgataggaaa agaaatttct gaagaggatg
      721 caaattatgc atctggtctt acagctgtat gttcgattat aaatggcggc aaacaattta
      781 actctgtacc agatgaagct tcacttgaat ttaacgtaag accagttcct gagtatgata
      841 acgactttat agaatcgttt ttccaaaata tcattaatga tgtggatagc aataagcttt
      901 cactcgatat tccaagcaat caccgacctg taacaagcga taaaaatagc aaattaatta
      961 ctacgattaa agatgtagct tctagttatg tagaacaaga cgaaatattt gtttcagcgc
     1021 ttgtaggtgc aacagatgcc tctagtttct taggagataa taaggacaat gttgatttag
     1081 ccatttttgg accaggtaat ccattaatgg cacatcaaat cgatgaatat attgaaaaag
     1141 atatgtacct gaaatatatt gatattttta aagaggcttc cattcaatat ttaaaagaga
     1201 aataagaacg atgctgtcag ctgccctatt cgcgtgctgg cagtttttta ttgcgaattt
     1261 taagttgata gaaatagcga tgaatattgt ttgactgtaa atattcaaat atcaacaagc
     1321 acattttcat tgattaagtg atgtaaaact gaaattatgg tggtgatttg tcatacatat
     1381 attgcattgt ctattgaaat tgggaatcca atttctcttt gttgaggccc ccaccccaac
     1441 ttacattgtt tgtagaattt cttttcgaaa ttctctgtgt tggggccccc accccaactt
     1501 gcattgtctg ttgaaattgg gaatccaatt tctctgtgtt ggggcccctg actagaattg
     1561 aaaagtaata cacatcagta acatttctat tttcatttat gatattatct aattattaat
     1621 ttataatata aaggagtatg caattaaaat gaacaaagtc cacaaacctt t
//
LOCUS       DAOBBH010000291         1640 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.293, whole genome shotgun sequence.
ACCESSION   DAOBBH010000291 DAOBBH010000000
VERSION     DAOBBH010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.293"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(78..413)
                     /locus_tag="P6L01_002579"
                     /pseudo
     CDS             complement(78..413)
                     /locus_tag="P6L01_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
     gene            complement(1064..1555)
                     /gene="sak"
                     /locus_tag="P6L01_002580"
     CDS             complement(1064..1555)
                     /gene="sak"
                     /locus_tag="P6L01_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylokinase"
                     /protein_id="HDP3031614.1"
                     /translation="MLKRSLLFLTVLLLLFSFSSITNEVSASSSFDKGKYKKGDDASY
                     FEPTGPYLMVNVTGVDGKGNELLSPHYVEFPIKPGTTLTKEKIEYYVEWALDATAYKE
                     FRVVELDPSAKIEVTYYDKNKKKEETKSFPITEKGFVVPDLSEHIKNPGFNLITKVVI
                     EKK"
BASE COUNT          540 a          301 c          237 g          562 t
ORIGIN      
        1 taaaatgatt atagcataat ttcaaactat tctataataa attaattaac ttcatatgaa
       61 actttacaca ctttttttca aagtgcgcta aaattaccaa aactgcttat tctattacct
      121 gccttgtcta cctctcctgt cgctatataa caacgttgtc cattattagc aatataagta
      181 atccatctat agccattaat gcaatatgcg ccgtcatatt tgatcgttgc gttattgggt
      241 aatacacctg taattcttga attagttgaa tagccatccc tcacgttatt acctttaaca
      301 ttggcaactg tgtaataacc agtctctttt ttatacggta cattgttttt atcgagtgta
      361 taacctgctg gcactggtga attcttttca tttttagctg gtgttttaac attactgata
      421 cctgatacac acttccaata aaaataacca caccatttaa gatgcggtgt agcgactgta
      481 atatttctat gttgttgaga tatatgtatc gagtgaaggg caaaaaggat atcaattgca
      541 ggataagtgt taggttacta ggccacttaa caggctatat agttcactcc tactatatac
      601 acttaattat aacataaaaa gcaccccgta aactattata cgggaatgct aaagtcatat
      661 atactacggg ggagtagtat gaaaactatg ctctctatca taagaaaaaa acacccagtg
      721 acatgcttgg gtgaacaagg atagatgtaa atagttgatg catgtgtaac acatcatagc
      781 aaaaaactag cccgaaagct agctataaca tacaatctaa aaagacgtcc tttgaatacg
      841 tctagaaaga ttataacata aaaaaatagg caagtaccga agtacctgcc taaaaaagga
      901 ttatccactt tttcatccta actgatttct ccccataagt cacctaatat ctgattaggt
      961 ggggaagaac cattcgtgca tgaatgagaa tttgatgaaa gataattttc actacacata
     1021 ttcaatcaag acattgcttt ctataataaa caactatttt gttttatttc ttttctataa
     1081 caacctttgt aattaagttg aatccagggt ttttaatatg ctctgataaa tctgggacaa
     1141 caaaaccttt ttctgttata gggaaagact tcgtttcttc ttttttctta ttcttatcat
     1201 aataagtgac ttcgatcttt gcgcttggat ctaattcaac tactctaaac tctttatatg
     1261 ctgtcgcatc taatgcccat tcgacatagt attcaatttt ttcttttgta agtgtagtcc
     1321 caggtttaat aggaaactcg acataatgag gggatagcaa ttcatttcct ttaccatcaa
     1381 ctccagtcac atttaccatc aaatacgggc ctgttggttc aaaataactc gcgtcatcgc
     1441 cttttttata ttttcctttg tcgaatgaac ttgatgcact tacctcatta gtaattgaag
     1501 aaaatgagaa taataacaat aaaacagtta aaaataataa acttcttttg agcatggcgc
     1561 ttcctccaaa tataatatat ttgtttttat ttatctcttt ttatagctaa cacccttatt
     1621 taagatgata aatatcaaca
//
LOCUS       DAOBBH010000292         1597 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.294, whole genome shotgun sequence.
ACCESSION   DAOBBH010000292 DAOBBH010000000
VERSION     DAOBBH010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.294"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(12..1493)
                     /gene="cls"
                     /locus_tag="P6L01_002581"
     CDS             complement(12..1493)
                     /gene="cls"
                     /locus_tag="P6L01_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDP3031615.1"
                     /translation="MRYTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASST
                     WAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGNVLTDFDGLLKQQIESFDKGNYG
                     TDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEY
                     YTFALDGLGKRILHALEEKLKQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFA
                     SKLPLLNFRMNNRNHRKIIVIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAV
                     DALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYT
                     KMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATFSNA
                     SDLLSSGVKIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSFELNFEVNAFVYDENLA
                     KDLRVAYEHDITKSKQLTKESYANRPLSVKFKESLAKLVSPIL"
BASE COUNT          547 a          291 c          206 g          553 t
ORIGIN      
        1 ttacaaataa attataaaat tggcgaaact aattttgcta acgattcttt gaatttaaca
       61 gacagcggtc tattggcata tgattctttg gttagttgtt ttgattttgt aatatcatgt
      121 tcataagcca cccttaaatc cttagcaaga ttttcatcat atacaaaggc atttacttca
      181 aaatttaatt caaaacttct aaagtccata tttgctgtgc ccactgatac gatttcatca
      241 tcaattaagc acattttaga atgtataaat ccattttcat acgtataaat tttaacacca
      301 cttgataata agtcagaggc atttgaaaat gtcgcccaat aaactaaagg atgatctggc
      361 ttacatggaa tcattagatg aacatctaca cctgatttag ctgcaatttt aatggcattt
      421 atatatgagt tatccggaat gaaataaggc gattgcaaat ataccgactt cttagcgctc
      481 ataatcattt ttgtataacc atattcaatt tgatgccagt cactagctgg gccacttgca
      541 gctatttgaa ttggtgaatt gcccaatggt ccgttctttt tagggaaata cttaacatca
      601 tattcaaact gtggacggtg cgcttgcgaa ttccagtcta aaataaatcg caactgcagt
      661 gcatcaaccg catccccttg tatacgtaaa tgcgtatctc tccaataccc tagtttacct
      721 aatcctagat attcatcacc aatgttaaat cctccgacat aacctagttg accatcgatt
      781 acgatgattt ttctatgatt tctgttattc atacggaaat ttaataaggg taatttagaa
      841 gcaaaaaatg cttcaacttc gccacctaat gatttaaagt gatcaaaatt tgccatctta
      901 acatttttag atccaacatc atcatataat atttttactt ctagaccttg tttcaatttt
      961 tcttctaaag catgtaaaat tcttttacct aaaccatcta aagcgaaagt atagtactct
     1021 aaatggatat attcttttgc atttttaata tcttttaaaa cttgatcata taaatcattt
     1081 ccatcaatga aatgatcaac tttattattt tcagttaaaa aaccatcttg atccatcaaa
     1141 agcatacgta ctaaatcatg atgtttttga acttgtttgt tatcagtacc ataattacct
     1201 ttatcaaagc tttctatttg ttgttttaaa agtccatcga aatccgttaa cacgttacca
     1261 ttgtttttat tcaatttacg tgccgaaacg gttctaccaa aaaacaagta aagaataaaa
     1321 ccaatcaatg gtaagacaaa aagtacaaat agccatgccc aagttgaact cgctgtacgt
     1381 ctatttcttt ctaaaaagat aataataaaa gccaatacta aattaatgat gaatccaatg
     1441 gctaaaataa tggtaaaaag tgttcctaaa tcatttgaaa atgtatatcg catagtcttt
     1501 tctcctataa agaaaggcac aaaaaacatt ttgcaccttt cacatcatat tatttattca
     1561 cagataaagt taaaattgca ttgaattctt cttcatt
//
LOCUS       DAOBBH010000293         1596 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.295, whole genome shotgun sequence.
ACCESSION   DAOBBH010000293 DAOBBH010000000
VERSION     DAOBBH010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.295"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(230..1255)
                     /locus_tag="P6L01_002582"
     CDS             complement(230..1255)
                     /locus_tag="P6L01_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HDP3031616.1"
                     /translation="MISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTAQAIEKI
                     KDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVISADTAHVAVHEGGAIEYSGHKV
                     ITIPSKEGKVSASDVETYMETFKSDFKKDHMVFPGMVYISHPTEYGTLYSKSELEELC
                     KVCKQYQLPLFMDGARLGYGLMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFT
                     KNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDNLYFNISRHAIEMANKMKDGFKN
                     KGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENL
                     NKLLELI"
BASE COUNT          546 a          273 c          208 g          569 t
ORIGIN      
        1 taatacttct caaatctcgt caaattaaag taaatacagc acaaattaac atttttaaat
       61 atataaaata aaacaagata ctcaataatc tgtagtatct tgttttggaa taatgtaatt
      121 ttatatcatc aacggcttca aatgagtagt ttcatataaa tcaataaaca caactactca
      181 actgatctta attaataaat gatttgtcag atgacacata acagcattat tagattaatt
      241 ctaatagttt gtttaaattt tcttcggttg tcgcccagct ggttgcgaat ctaacaacac
      301 gatgttgatc atcgtatttt tcccaaacag caaatttaac tttttgttct aactctgcta
      361 ttttctcgtt acttaaaata aaaaattgtt gattggttgg agaatcaaag taaagacgat
      421 agcctttatt tttaaacccg tctttcatct tatttgccat ttcgatagca tgtctgctta
      481 tattaaaata taaattgtcc gtaaataatt ctaaaaattg tatgcctgtt aaccgtcctt
      541 ttgctaaaag ggcaccgtga tgtttgattc gagtggtaaa ttgtttcggt tcattatttt
      601 tcgtaaaaac aatggcttcc ccgcataatg cacctatctt cgtaccacct atataaaata
      661 catcacaata tttagcgatg tctttaatag tcatatctga ttgatcactc atcaatccat
      721 accctaatcg tgcaccatcc ataaataatg gaagctgata ttgcttacat actttgcata
      781 actcttccaa ttctgattta gagtataaag tgccatattc tgtaggatga gaaatatata
      841 ccattcctgg gaataccata tggtcctttt taaaatcact tttaaatgtc tccatgtaag
      901 tttcaacatc tgaagcacta acttttcctt ccttagaggg tatagtaatt actttatgtc
      961 cactatattc aattgcacca ccctcatgca cagcaacatg agcagtgtct gctgaaatga
     1021 ccccttcgta actttctaac attgaattaa taacaacctg attggtttgt gttccaccta
     1081 ctaaaaaacg aattgtagca tttggacaat caattgtatc tttaatcttt tcaattgcct
     1141 gagctgtgaa ttgatcaaag ccatatcccg aagcttgtac aagatttgta tctactaatc
     1201 gttttaatac tttttcatga gcaccttcta aataatcatt ttcaaatgag atcactacat
     1261 ttccccctaa aactaatatc agcattttaa tatgataaac taatttcaaa actagttcga
     1321 tatttaaaat gtattgtgaa ttgttaaagt ttgtatcgca ttatcgcgaa gttgaataaa
     1381 tatattatac atcaaacaat acattaataa aattaattaa tttttaatca tagcgacttt
     1441 tcatgctgga acacatatat taatgattga attttactgt taaaagttct atgatttagg
     1501 aaatagagaa aagaggggat ttaaatggaa caaattaaac ttaaaacttt tacagctgag
     1561 actttagagt tattagaaaa aaacattaat gctttt
//
LOCUS       DAOBBH010000294         1572 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.296, whole genome shotgun sequence.
ACCESSION   DAOBBH010000294 DAOBBH010000000
VERSION     DAOBBH010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.296"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..618
                     /locus_tag="P6L01_002583"
     CDS             <1..618
                     /locus_tag="P6L01_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDP3031617.1"
                     /translation="EGMQTIKKITRIASDIGVKYLTLYAFSTENWSRPESEVNYIMNL
                     PVNFLKTFLPELIEKNVKVETIGFTDKLPKSTIEAINNAKEKTANNTGLKLIFAINYG
                     GRAELVHSIKNMFDELHQQGLNSDIIDETYINNHLMTKDYPDPELLIRTSGEQRISNF
                     LIWQVSYSEFIFNQKLWPDFDEDELIKCIKIYQSRQRRFGGLSEE"
     gene            625..1407
                     /locus_tag="P6L01_002584"
     CDS             625..1407
                     /locus_tag="P6L01_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDP3031618.1"
                     /translation="MKVRTLTAIIALIVFLPILLKGGLVLMIFANILALIALKELLNM
                     NMIKFVSVPGLISAVGLIIIMLPQHAGPWVQVIQLKSLIAMSFIVLSYTVLSKNRFSF
                     MDAAFCLMSVAYVGIGFMFFYETRSEGLHYILYAFLIVWLTDTGAYLFGKMMGKHKLW
                     PVISPNKTIEGFIGGLFCSLIVPLAMLYFVDFNMNVWILLGVTLILSLFGQLGDLVES
                     GFKRHFGVKDSGRILPGHGGILDRFDSFMFVLPLLNILLIQS"
BASE COUNT          532 a          195 c          291 g          554 t
ORIGIN      
        1 gaaggtatgc aaacaataaa aaaaattact aggatagcta gtgatattgg tgttaagtac
       61 ttaactttat atgccttttc cactgaaaat tggtcaagac ctgaaagtga agtaaattat
      121 attatgaatt tgcctgtcaa tttcttaaag acattcttac cggaactaat tgaaaaaaat
      181 gtcaaagttg aaacaattgg atttactgat aagttgccaa aatcaacgat agaagcaatt
      241 aataatgcta aagaaaagac agctaataat accggcttaa aattaatatt tgcaattaat
      301 tatggtggca gagcagaact tgttcatagt attaaaaata tgtttgacga gcttcatcaa
      361 caaggtttaa atagtgatat catagatgaa acatatataa acaatcattt aatgacaaaa
      421 gactatcctg atccagagtt gttaattcgt acttcaggag aacaaagaat aagtaatttc
      481 ttgatttggc aagtttcgta tagtgaattt atctttaatc aaaaattatg gcctgacttt
      541 gacgaagatg aattaattaa atgtataaaa atttatcagt cacgtcaaag acgctttggc
      601 ggattgagtg aggagtagta tagtatgaaa gttagaacgc tgacagctat tattgcctta
      661 atcgtattct tgcctatctt gttaaaaggc ggccttgtgt taatgatatt tgctaatata
      721 ttagcattga ttgcattaaa agaattgttg aatatgaata tgattaaatt tgtttcagtt
      781 cctggtttaa ttagtgcagt tggtcttatc atcattatgt tgccacaaca tgcagggcca
      841 tgggtacaag taattcaatt aaaaagttta attgcaatga gctttattgt attaagttat
      901 actgtcttat ctaaaaacag atttagtttt atggatgctg cattttgctt aatgtctgtg
      961 gcttatgtag gcattggttt tatgttcttt tatgaaacga gatcagaagg attacattac
     1021 atattatatg cctttttaat tgtttggctt acagatacag gggcttactt atttggtaaa
     1081 atgatgggca aacataagct ttggccagta ataagtccga ataagacaat cgaaggattc
     1141 ataggtggct tgttctgtag tttgatagtg cctcttgcaa tgttatattt tgtagatttc
     1201 aatatgaatg tatggatatt acttggagtg acattgattt taagtttatt tggtcaatta
     1261 ggtgatttag tggaatcagg atttaagcgt catttcggcg ttaaagactc aggtcgaata
     1321 ctacctggac acggtggtat tttagaccga tttgacagct ttatgtttgt gttaccatta
     1381 ttaaatattt tattaataca atcttaatgc tgagaacaaa tcaataaacg taaagaggag
     1441 ttgctgagat aatttaatga atctcagaac tccttttgaa aattatacgc aatattaact
     1501 ttgaaaatta gacgttatat tttgtgattt gtcagtatca tattataatg acttatgtta
     1561 cgtatacagc aa
//
LOCUS       DAOBBH010000295         1569 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.297, whole genome shotgun sequence.
ACCESSION   DAOBBH010000295 DAOBBH010000000
VERSION     DAOBBH010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.297"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            457..1416
                     /locus_tag="P6L01_002585"
     CDS             457..1416
                     /locus_tag="P6L01_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDP3031619.1"
                     /translation="MNRNIVKLVVFMLILVVAVAGCGQKDTEEKTEMTTIKDELGTEK
                     IKKNPKRVVVLEYSFADYLAALDMKPVGIADDGSTKNITKSVRDKIGAYESVGSRPQP
                     NMEVISKLKPDLIIADVSRHKKIKSELSKIAPTIMLVSGTGDYNANIEAFKTVAKAVG
                     KEKEGEKRLEKHDKILAEIRKKIEQSTLKSAFAFGISRAGMFINNEDTFMGQFLIKMG
                     IQPEVTKDKTTHVGERKGGPYIYLNNEELANINPKVMILATDGKTDKNRTKFIDPAVW
                     KSLKAVKDNKVYDVDRNKWLKSRGIIASESMAEDLEKIAEKAK"
     gene            complement(1462..>1569)
                     /locus_tag="P6L01_002586"
     CDS             complement(1462..>1569)
                     /locus_tag="P6L01_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031620.1"
                     /translation="FIKRTVKTILVGYVIKFIRNKLSGKSSHPTDNKHN"
BASE COUNT          620 a          171 c          306 g          472 t
ORIGIN      
        1 catagtccaa attatatttg taaacatgtg gaaagtaaag attttttata agattaatta
       61 ttaaatattt attaaatatt ctattgtatt gaacaattgt tgtagaaaga tgtttaagtc
      121 atcagttact ttttgtattt ataaataaag attagtacag catagtgcaa agattatagg
      181 gaaatgaatt aactattgca tcgctattta tgaatcgtaa acactattat gaaattgcga
      241 gagtagtatg gataattaga atgatggttt gagataaatg agtattatta accagtgcgt
      301 catcttcaaa attagaggcc atttgctgtt tataataaga agatttgata ataaactatg
      361 ataagaaaaa ataatagcat accaaatata tcataaacaa attaaatgat aaaataataa
      421 tgcagttttc tattaaagtt gtttggaggt aaatatgtga ataggaatat cgttaaatta
      481 gttgtgttta tgctaatctt agttgtagca gtagcgggtt gtggtcaaaa agatactgaa
      541 gagaaaactg aaatgacgac aataaaagat gaattaggaa ctgaaaaaat taagaaaaat
      601 cctaaacgtg ttgttgtatt agaatatagt tttgctgatt atttagcagc attagatatg
      661 aaacctgttg gtattgcaga tgatggcagc actaaaaata taacaaagtc agtaagagat
      721 aagattgggg catatgaatc ggttggatct agaccgcaac cgaatatgga agtgataagt
      781 aaattaaaac cggatttgat cattgcagat gttagcagac ataagaaaat caaatcagaa
      841 ttgagcaaaa ttgctccgac aatcatgtta gttagcggta cgggagatta taatgcaaat
      901 attgaagcat ttaaaacagt cgctaaagca gtaggcaaag agaaagaagg cgagaagcgt
      961 ctggaaaagc atgataaaat attagcggag attagaaaga aaattgaaca gagtacgtta
     1021 aaatctgcat ttgcattcgg tatctcaaga gcaggtatgt ttattaataa tgaagataca
     1081 tttatgggac aattcttaat taaaatgggt attcaacctg aagtcacaaa agacaaaact
     1141 acgcatgttg gtgaacgcaa gggtggtcct tatatatatt taaataatga agaacttgcc
     1201 aatatcaatc caaaagttat gattttagcc actgacggaa aaacggacaa aaatagaacg
     1261 aaattcattg atcctgcagt ttggaaatca ttaaaagctg tgaaagataa caaagtttat
     1321 gacgttgacc gaaataagtg gttgaaatca agggggatta tcgcaagtga aagtatggca
     1381 gaagatttag aaaaaattgc agaaaaagca aaataaaaat acagcgctac tcgtaaatca
     1441 tataagagtg gcgctgtatt tttaattatg tttattatct gtcggatgtg atgatttacc
     1501 tgaaagttta tttcgaataa atttaattac ataaccgaca aggattgttt taacagttct
     1561 tttaatgaa
//
LOCUS       DAOBBH010000296         1560 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.298, whole genome shotgun sequence.
ACCESSION   DAOBBH010000296 DAOBBH010000000
VERSION     DAOBBH010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.298"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            64..1518
                     /locus_tag="P6L01_002587"
     CDS             64..1518
                     /locus_tag="P6L01_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDP3031621.1"
                     /translation="MEDNKMNRSLNSRHISMIAIGGAIGTGLFVATGNIISQAGPGGA
                     ILAYLVIGVMLYFLMSSIGELATFYPVSGSFSSYSTRFIDSSLGFTMGWLYWALWSLV
                     TSVDVIVASNVLYFWDTFKFFHPITWSLIFITILLLLNIFSVKSFGETEFWLSLIKVL
                     TIIVFVIFGFLMIFGILGGHTYGFENYTKGQAPFVGGISGFLGVLLVAGFSVGGTEVV
                     AVTAGESDDPKKSMPKAIKQVFWRILLFYVLSIAVIGAIIPYTDPSLLRASSSISQSP
                     FTIVFDRVGIAFAASVINAVILTSLLSAANSGVYTTGRMLYSLSSDKKAPQFLSKLNK
                     TTKLPLRALLTTYAVVVIVIIYANFNSNAVFNLLEIIGSMIIVVWGSSIWSQIRLRQA
                     IKKQGQDPNKVLPYKAPFYPLGPIIVITTLLFLLFGGSVEYILKDQWLNAFKNFLPLI
                     ILALIYFIHKIIHKTKFVKLETINLKPHDYDNQK"
BASE COUNT          488 a          250 c          262 g          560 t
ORIGIN      
        1 caaaaataat agccttaaaa agcttacaat aatgggtata tttaaataga tgggagacta
       61 aatatggaag ataataaaat gaaccgtagt cttaactcaa gacacatttc catgattgct
      121 ataggtggtg caattgggac tggtctattt gtagctactg gtaatatcat ttctcaagct
      181 ggtcctggag gcgctatact cgcttatctt gttattggtg tcatgctata tttcttaatg
      241 tcatcaattg gagagttggc aacattttat ccagtatcag gttcattcag ctcttattca
      301 acacgcttta ttgactcatc tcttggcttt accatgggat ggttgtattg ggcattgtgg
      361 tcattagtta caagtgttga tgtcatagta gcgtcaaatg tgctttattt ctgggacaca
      421 tttaaatttt tccaccccat tacttggagc ttaatcttta ttacaatttt actattatta
      481 aacatttttt ctgtaaaatc atttggagaa actgagtttt ggttatcatt gattaaagtg
      541 ttaacaatta tcgtattcgt tatttttggc tttttaatga ttttcggtat cttaggtggt
      601 catacatatg gatttgaaaa ctatacaaaa ggccaagcac cgtttgttgg tggtatctct
      661 ggtttcttag gcgtattatt agtcgccgga ttttcggttg gtggtacaga agtagtagca
      721 gtaactgctg gtgaatcaga tgaccctaaa aagtctatgc ctaaggcaat taaacaagta
      781 ttttggcgta ttcttttatt ctatgtctta tcaattgcag taattggtgc aattattccg
      841 tacacagatc catcattatt aagagcaagt agttcaataa gtcaaagccc atttacaatt
      901 gtattcgata gagtaggcat agcctttgca gcatcagtaa tcaacgcggt tattttaact
      961 tcattattat ccgctgcaaa ttcaggtgtt tatacaacag gcagaatgtt gtattcctta
     1021 agttcagaca aaaaagcacc ccaattttta agtaaattaa acaagacaac taagttacct
     1081 ttaagagcat tattaactac ttatgcagtc gttgttattg ttattattta tgcaaacttt
     1141 aattcaaatg ccgtttttaa tttacttgaa attattggtt caatgattat agttgtttgg
     1201 ggatcaagca tttggtcaca aatacgattg cgacaagcta ttaaaaaaca aggtcaagac
     1261 cctaataagg tcctaccata taaagcacct ttttatccat taggaccaat cattgtcatc
     1321 actacactat tattcttgct atttggtggc tcagttgaat atattttaaa agatcaatgg
     1381 ttaaatgctt ttaaaaactt tttaccttta atcattctag cgttgattta ctttattcat
     1441 aaaatcattc acaaaacaaa atttgtaaag ctagaaacaa ttaatttaaa accacacgat
     1501 tatgacaatc aaaaataaat aaattcttac acggcagatg ctttagatta tctatagcat
//
LOCUS       DAOBBH010000297         1542 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.299, whole genome shotgun sequence.
ACCESSION   DAOBBH010000297 DAOBBH010000000
VERSION     DAOBBH010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.299"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..487)
                     /locus_tag="P6L01_002588"
     CDS             complement(<1..487)
                     /locus_tag="P6L01_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDP3031622.1"
                     /translation="MVCEVIAIYKNPKYRIIKYNDEYLMVNIINNWLVLFIPLLNWLT
                     PKRYIKISQEELESLNTFKPAKNNAFWPALGSSVLFSVTFRKYMPLFNVRLEKTIVIA
                     IFFVVFLGILFFYLNLNRRLALSVFTMNKEKSQKMILLPNLKHFFFIIFAYLYFGGFS
                     FF"
     gene            complement(627..1247)
                     /locus_tag="P6L01_002589"
     CDS             complement(627..1247)
                     /locus_tag="P6L01_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDP3031623.1"
                     /translation="MLCESKIINKNPKYRIIKYNDEYLMIDIISTWISLFFPFINWFI
                     PKRYVKISREEFENLNIVKPAKKNVFWPVASISTLFAVTLRKYTLLLDTQLDKKLVIA
                     ICCTTFIGILIFYVRLIKKSSLNIYNTKNKRSKIILIPTLKNFCFTLFGYVFLIFWTV
                     IFSYALLSMSYQNIIVYFAWITAIIGFFLVNIALIIDKNIHVILKN"
     gene            complement(1294..>1542)
                     /locus_tag="P6L01_002590"
     CDS             complement(1294..>1542)
                     /locus_tag="P6L01_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDP3031624.1"
                     /translation="KENKNNELKLRLLPSFKNMCFTIFYYLFTGFMSYGAFYLLVFEN
                     VQNLILYISWLFMTMLFMLMNMHSIIDKKVHIFLKSNK"
BASE COUNT          597 a          209 c          171 g          565 t
ORIGIN      
        1 tgaaaaatga aaaaccaccg aaatataaat atgcaaatat tataaagaaa aaatgtttta
       61 aattaggtag taatatcatt ttctgacttt tttctttatt catagtaaaa acacttaatg
      121 ctagcctacg atttaaattt aggtagaaaa ataaaatacc aagaaataca acaaaaaata
      181 tggctattac tattgttttt tccaatctaa cattaaataa aggcatatac tttctaaaag
      241 tcacactaaa taaaactgaa ctacctaatg ctggccaaaa tgcattgttt ttagcaggtt
      301 tgaaagtatt taaactttct aattcttctt gactgatctt gatgtatctt tttggtgtca
      361 accaattaag caatggaatg aataaaacta accaattatt tattatattc accatcaaat
      421 attcatcatt atatttaata attctatatt tgggattttt gtatatagct attacctcgc
      481 aaaccaatgt ctccacatcc ctttaatttt atgtgtaata catttttcga tacttcaaaa
      541 gacattaaaa tattatcaac ttactatcat taaaaatttt attcattgtt atttgcatat
      601 ctttaatctg aatttattac caccatttaa tttttaagta tgacatgaat gtttttatct
      661 ataattaaag ctatattcac tagaaaaaaa cctattattg ctgtaatcca agcaaaatat
      721 actattatgt tttgataact cattgataat agagcatatg agaaaatcac tgtccaaaaa
      781 attaaaaaaa catatccgaa caatgtgaaa caaaaatttt ttagtgttgg tattaagatg
      841 attttcgacc ttttattttt agtattataa atatttaaag atgatttttt aattaggcgt
      901 acataaaata ttaaaatccc tataaatgtg gtgcaacata tggcaataac taatttttta
      961 tcaagttgag tgtcaagtaa aagcgtatac tttcttaatg taactgcgaa taaagtagag
     1021 atactagcaa ctggccaaaa aacattcttt ttagcaggtt taacaatatt taaattttca
     1081 aactcttctc tactaatttt gacgtatctt ttggggatga accaattaat aaaaggaaaa
     1141 aataaactaa tccaagtgct tattatatcg atcattaagt actcatcatt atatttaata
     1201 attctatatt ttgggttttt attgatgatt ttagattcgc acagcaatgt ttacaccccc
     1261 taattagcaa ttgaaaacca actaaatttg taactattta ttagacttta aaaatatatg
     1321 tactttttta tctataattg agtgcatatt cattaacata aatagcatag tcatgaaaag
     1381 ccaagaaata tataagatta aattttgtac attttcaaac accaacaagt aaaatgcccc
     1441 ataagacatg aaaccagtaa agaggtaata aaaaattgtg aaacacatat ttttaaagga
     1501 gggtaataat cttaatttaa gttcattatt tttattttcc tt
//
LOCUS       DAOBBH010000298         1517 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.300, whole genome shotgun sequence.
ACCESSION   DAOBBH010000298 DAOBBH010000000
VERSION     DAOBBH010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.300"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            130..624
                     /locus_tag="P6L01_002591"
                     /pseudo
     CDS             130..624
                     /locus_tag="P6L01_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777469.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            615..776
                     /locus_tag="P6L01_002592"
                     /pseudo
     CDS             615..776
                     /locus_tag="P6L01_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(754..855)
                     /locus_tag="P6L01_002593"
     CDS             complement(754..855)
                     /locus_tag="P6L01_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031625.1"
                     /translation="MQGFKEKHQELKKALCQIGLMSSISEVRQLNIA"
     gene            complement(978..1073)
                     /gene="pepA1"
                     /locus_tag="P6L01_002594"
     CDS             complement(978..1073)
                     /gene="pepA1"
                     /locus_tag="P6L01_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin
                     PepA1"
                     /protein_id="HDP3031626.1"
                     /translation="MMLIFVHIIAPVISGCAIAFFSYWLSRRNTK"
BASE COUNT          591 a          223 c          250 g          453 t
ORIGIN      
        1 aaaatactga gtcattcaat catcaagaca gtttaactgg aaatgataat ttaataatgt
       61 gcacttttga ttgtttaaac atgtacaatt tttaaaaacc tatagataat tttattgaga
      121 tgaacaacaa tgaaattatc tttagacata aatactgatt ttgaagttac tactcttaca
      181 gatttaccaa aattaaaaat tgtcatggag aacttaaaca tgaaaataaa caaaagtgaa
      241 atagcgagac acatgggtgt ttatagaaga acagttgata aatatttaaa tggctttgag
      301 ccaaccaaaa agagaaatcg tcagtctatt attgacaagt attatcctat catcgaaaaa
      361 ttactttcag actctagtga acaaaagttc tactataagc ttattctttg gcaatatttg
      421 aaagataaac atggattaac ctgtgcatat tctacattta gagcttatat ccttaaacat
      481 gatgaattta atcgttattt tatgaaaggt tatcaacggt tgtcgcctaa aggcaaaacg
      541 agattcgaga caaaagccag tcatcaagca cagtttgact ggaaagaagg cattaacttt
      601 aaaacgaagg ataatcaaat ggtcctttag aagggataaa caacaaaata aaattaatta
      661 aacgtacatc ttttggttaa ggaagttata atcatttgcg aaatcgaata ttattatgtt
      721 caaaacttta cgctccaaaa agtaaaaagg aagctaagca atgtttagtt gcctaacttc
      781 cgatattgaa ctcatcaggc caatttggca tagagccttt tttagttctt gatgtttctc
      841 tttaaaacct tgcatatttt acaaagagaa agattagcag tataattgag ataacgaaaa
      901 taagtattta cttatacacc aatcccctca ctatttgcgg tagtgagggg atttttattg
      961 gtgcggctat atgtcaccta ttttgtattg cgtctactta gccaataaga aaaaaacgca
     1021 atggcacagc cactgatgac tggtgctatg atgtgaacga aaataagcat caccttatac
     1081 acctcctctc tgcgtctaaa ttgacgcctg agagataggc gactctacta ttatatcatc
     1141 taattggaca aattctatga gagtagattt tgttaattta agaaagaaga acatttagtt
     1201 cagagtttaa gttataaatg gttagattat atgaaaatgg taagccaaga aatgaaatta
     1261 tacgcgagta tgatttaaca cctttgacgt taggaaaatg gataaagtaa catcaaaaca
     1321 agggtacatt caatcaccaa gataacttaa cagatgaaga aaaagagctg attatggggc
     1381 aaaaatagaa gtcattcaaa agaatgcaca tcaatattca gtaccagcaa tgtgtaaagt
     1441 cctaaaaata ccaagaagta cctattatga ttctataaaa agaaaagata ataaaatcac
     1501 taaagatgat tcaaacg
//
LOCUS       DAOBBH010000299         1516 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.301, whole genome shotgun sequence.
ACCESSION   DAOBBH010000299 DAOBBH010000000
VERSION     DAOBBH010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.301"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(6..>1516)
                     /locus_tag="P6L01_002595"
     rRNA            complement(6..>1516)
                     /locus_tag="P6L01_002595"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          332 a          440 c          334 g          410 t
ORIGIN      
        1 cttagaaagg aggtgatcca gccgcacctt ccgatacggc taccttgtta cgacttcacc
       61 ccaatcattt gtcccacctt cgacggctag ctcctaaaag gttactccac cggcttcggg
      121 tgttacaaac tctcgtggtg tgacgggcgg tgtgtacaag acccgggaac gtattcaccg
      181 tagcatgctg atctacgatt actagcgatt ccagcttcat gtagtcgagt tgcagactac
      241 aatccgaact gagaacaact ttatgggatt tgcttgacct cgcggtttcg ctgccctttg
      301 tattgtccat tgtagcacgt gtgtagccca aatcataagg ggcatgatga tttgacgtca
      361 tccccacctt cctccggttt gtcaccggca gtcaacttag agtgcccaac ttaatgatgg
      421 caactaagct taagggttgc gctcgttgcg ggacttaacc caacatctca cgacacgagc
      481 tgacgacaac catgcaccac ctgtcacttt gtcccccgaa ggggaaggct ctatctctag
      541 agttgtcaaa ggatgtcaag atttggtaag gttcttcgcg ttgcttcgaa ttaaaccaca
      601 tgctccaccg cttgtgcggg tccccgtcaa ttcctttgag tttcaacctt gcggtcgtac
      661 tccccaggcg gagtgcttaa tgcgttagct gcagcactaa ggggcggaaa ccccctaaca
      721 cttagcactc atcgtttacg gcgtggacta ccagggtatc taatcctgtt tgatccccac
      781 gctttcgcac atcagcgtca gttacagacc agaaagtcgc cttcgccact ggtgttcctc
      841 catatctctg cgcatttcac cgctacacat ggaattccac tttcctcttc tgcactcaag
      901 ttttccagtt tccaatgacc ctccacggtt gagccgtggg ctttcacatc agacttaaaa
      961 aaccgcctac gcgcgcttta cgcccaataa ttccggataa cgcttgccac ctacgtatta
     1021 ccgcggctgc tggcacgtag ttagccgtgg ctttctgatt aggtaccgtc aagatgtgca
     1081 cagttactta cacatatgtt cttccctaat aacagagttt tacgatccga agaccttcat
     1141 cactcacgcg gcgttgctcc gtcaggcttt cgcccattgc ggaagattcc ctactgctgc
     1201 ctcccgtagg agtctggacc gtgtctcagt tccagtgtgg ccgatcaccc tctcaggtcg
     1261 gctatgcatc gttgccttgg taagccgtta ccttaccaac tagctaatgc agcgcggatc
     1321 catctataag tgacagcaag accgtctttc acttttgaac catgcggttc aaaatattat
     1381 ccggtattag ctccggtttc ccgaagttat cccagtctta taggtaggtt atccacgtgt
     1441 tactcacccg tccgccgcta acatcagaga agcaagcttc tcgtccgttc gctcgacttg
     1501 catgtattag gcacgc
//
LOCUS       DAOBBH010000300         1512 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.302, whole genome shotgun sequence.
ACCESSION   DAOBBH010000300 DAOBBH010000000
VERSION     DAOBBH010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.302"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..743
                     /gene="map"
                     /locus_tag="P6L01_002596"
     CDS             <1..743
                     /gene="map"
                     /locus_tag="P6L01_002596"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDP3031627.1"
                     /translation="EELQALKEIGYICAKVRNTMQAATKPGITTKELDNIAKELFEEY
                     GAISAPIHDENFPGQTCISVNEEVAHGIPSKRVIREGDLVNIDVSALKNGYYADTGIS
                     FVVGESDDPMKQKVCDVATMAFENAIAKVKPGTKLSNIGKAVHNTARQNDLKVIKNLT
                     GHGVGLSLHEAPAHVLNYFDPKDKTLLTEGMVLAIEPFISSNASFVTEGKNEWAFETS
                     DKSFVAQIEHTVIVTKDGPILTTKIEEE"
     gene            960..1346
                     /locus_tag="P6L01_002597"
     CDS             960..1346
                     /locus_tag="P6L01_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031628.1"
                     /translation="MNYVERYIEQFLRATVRNNIKHYLLMLDEKMKNLDDYMRYLITK
                     KEQLSKLIDSLMLTLENKYIDIAEAFQIQCAREINNQEIENIKSELNKVEAYYAQIET
                     QIQQTSTEKIATEKTSYLINYMNAVA"
     gene            1361..>1512
                     /locus_tag="P6L01_002598"
     CDS             1361..>1512
                     /locus_tag="P6L01_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDP3031629.1"
                     /translation="MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLV
                     YVGYLY"
BASE COUNT          578 a          203 c          272 g          459 t
ORIGIN      
        1 aagaagaatt acaagcgtta aaagaaattg gatacatatg cgctaaagtg cgcaatacaa
       61 tgcaagctgc aaccaaacca ggtatcacta cgaaagagct tgataatatt gcgaaagagt
      121 tatttgaaga atacggtgct atttctgcgc caattcatga tgaaaatttt cctggtcaaa
      181 cgtgtattag tgtcaatgaa gaggtggcac atgggattcc aagtaagcgt gtcattcgtg
      241 aaggagattt agtaaatatt gatgtatcgg ctttgaagaa tggctattat gcagatacag
      301 gcatttcatt tgtcgttgga gaatcagatg atccaatgaa acaaaaagta tgtgacgtag
      361 caacgatggc atttgagaat gcaattgcaa aagtaaaacc gggtactaag ttaagtaaca
      421 ttggtaaagc ggtgcataat acagctagac aaaatgattt gaaagtcatt aaaaacttaa
      481 caggtcatgg tgttggttta tcattacatg aagcaccagc acatgtactt aattactttg
      541 atccaaaaga caaaacatta ttaactgaag gtatggtatt agctattgaa ccgtttatct
      601 catcaaatgc atcatttgtt acagaaggta aaaatgaatg ggcttttgaa acgagcgata
      661 aaagttttgt tgctcaaatt gagcatacgg ttatcgtgac taaggatggt ccgattttaa
      721 cgacaaaaat tgaagaagaa tagttcaaca tatactaaga ctaaagtatg aacatcattt
      781 agttccggag cctattcata ttggtttcgg aactgtttta taataattaa gaacacaatc
      841 aatgcgtcat ttcaaaaata tgttgtaata aagtagtttt taagcaaaca tatcatcgac
      901 atcaacgaag atacatagcg catttggtat tttaaaactt attataaaag gtgatagtta
      961 tgaactatgt tgaacgttat attgaacagt ttttgagagc aacagtaaga aataatatca
     1021 agcactacct tttaatgcta gatgaaaaaa tgaaaaattt agatgattat atgcgttatt
     1081 taattactaa aaaagaacaa cttagcaagt taattgacag tctaatgcta acattagaaa
     1141 ataaatatat tgatattgct gaagcatttc aaattcaatg tgcaagagaa atcaataatc
     1201 aagaaattga aaatattaaa tcagagttga ataaagttga agcatattat gcacaaattg
     1261 aaactcaaat tcaacaaact tcaactgaaa aaatagcaac agaaaaaaca tcgtatctaa
     1321 taaattatat gaacgctgtg gcatagaaag gcggcgaaac atgacacaca aatatatatc
     1381 aacgcaaatg ttgatcattt ttactgcatt aatgattatt gccaattttt actacatatt
     1441 ttttgaaaaa attggctttt tactcgttct attattggga tgtgtattag tttatgtagg
     1501 atatctttat tt
//
LOCUS       DAOBBH010000301         1467 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.303, whole genome shotgun sequence.
ACCESSION   DAOBBH010000301 DAOBBH010000000
VERSION     DAOBBH010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.303"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            77..1467
                     /locus_tag="P6L01_002599"
                     /pseudo
     CDS             77..1467
                     /locus_tag="P6L01_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT          571 a          210 c          227 g          459 t
ORIGIN      
        1 ggcactgctc cctcaggagt ctcgccatta atactacgta ttaacatgta attttacttt
       61 tacatacttt aaaaaaataa gacactttgc ccaacttaca ctaccaatag aaacttctgt
      121 tagaattcct taaaatgata tttcgcgata tgtaaatgaa attgttgaaa cgatacctga
      181 tagcgaattc gatgaattca gacatcatcg tggcgcaaca tcctatcatc caaaaatgat
      241 gttaaaaatc atcttatatg catatactca atctgtattt tctggtcgta gaatagagaa
      301 attacttcat gacagtattc gaatgatgtg gttagctcaa gatcaaacac cttcttataa
      361 aactattaat cgttttagag tgaatcctaa tactgatgcg ttaattgaat ctttatttat
      421 tcagttccat agtcaatgtt taaagcaaaa tcttattgat aataattcaa tttttattga
      481 tggtacataa gtagaagcta atgccaatag atatacattt gtgtggaaga aaagtattca
      541 aaatcacgag aagaaataga tttaattggt agtcatttag ataaagaaat cgaagattta
      601 aatcattcta tttagaacga agattgtgct caaattagaa aacagacccg taaaaaagaa
      661 ctgagattaa gaagttcaaa aagaaatttg atgattattc cgaaagaaaa aataaatatg
      721 aagaacaaaa atcgattctt aaagatagaa atagcttttc taaaactgat catgatgcaa
      781 cttttatgag aatgaaggaa gaccatatga aaaatggcca acttaagcca ggatacaatt
      841 tacaaatagc gacaaattct caattttttt atcctatgac ctatttcaaa ccccaacaga
      901 tactagaact ttaattccat ttttaacaat gattcaaaat accttcggtt atttaccaga
      961 gtatattgta gctgatgcag gttatgatag tgagcaaaac tatatggcta ttatagatga
     1021 ttttaataaa acgccactta ttacgtatgg catgtttatt aaagataaaa ctagaaagtt
     1081 taaaagtgac atttttaaca ctcaaaattg gaaatatgac gaacttaatg atgaatttat
     1141 atgtcctaat aacaaaagaa taggttttaa aagatatgca taccgtaatg atagatatgg
     1201 ttttaaacgt gacttcaagc tatatgaatg cgatgactgt tcagcatgtt ctttgaggca
     1261 acaatgcatg aaaccaaatt cgaaatccaa taagaaaatc atgaagaatt ataattggga
     1321 atactttaaa gcccaaatta atcaaaagct ttctgaacca gaaacgaaaa aaatctatag
     1381 tcaaagaaaa attgatgtag agcttgtttt tggatttatg aaggctattt tgggtttcac
     1441 tcgaatgtca gttcgaggaa taaataa
//
LOCUS       DAOBBH010000302         1450 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.304, whole genome shotgun sequence.
ACCESSION   DAOBBH010000302 DAOBBH010000000
VERSION     DAOBBH010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.304"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            351..752
                     /locus_tag="P6L01_002600"
     CDS             351..752
                     /locus_tag="P6L01_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl peptide receptor-like 1 inhibitory
                     protein"
                     /protein_id="HDP3031630.1"
                     /translation="MKKNITKTIIASTVIAAGLLTQTNDAKAFFSYEWKGLEIAKNLA
                     DQAKKDDERIDKLMKESDKNLTPYKAETVNDLYLIVKKLSQGDVKKAVVRIKDGGPRD
                     YYTFDLTRPLEENRKNIKVVKNGEIDSIYWD"
     gene            712..804
                     /locus_tag="P6L01_002601"
                     /pseudo
     CDS             712..804
                     /locus_tag="P6L01_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499655.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1121..1450)
                     /gene="ecb"
                     /locus_tag="P6L01_002602"
     CDS             complement(1121..1450)
                     /gene="ecb"
                     /locus_tag="P6L01_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Ecb"
                     /protein_id="HDP3031631.1"
                     /translation="MKKNFIGKSILSIAAISLTVSTFAGESHAQTKNVEAAKKYDQYQ
                     TNFKKQVNKKVVDAQKAVNLFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLV
                     LKYNTLK"
BASE COUNT          518 a          218 c          198 g          516 t
ORIGIN      
        1 aaaaaatata tatcaaaaaa gctatatgta taacaatttg gagtgtttgt ctacccaaca
       61 ttgcaaacaa aatgcaaaga aatatatatc gttttaacaa taaaaattat ccaatcatag
      121 ttgagtgacg tttactacta ttattatact atcaacaaat tcaataatac ctcaaataaa
      181 aataatacca tcaaattcat cattattctt tttcgattgc attaaacctt attaatttgc
      241 acttaatttt aattaatgaa gtgttaactg gtaaatgcat gaaaccgttt gtaaaatagc
      301 agtgtactct cattagtaag tacaaattta ttttacaggg agaacttatt atgaaaaaaa
      361 atatcacaaa aactattatc gcatctacag taatcgctgc aggtctttta actcaaacta
      421 atgatgctaa agctttcttt agttatgaat ggaaaggttt agaaatcgca aaaaatttag
      481 cagatcaagc taaaaaggac gatgaacgta ttgataagtt aatgaaagag tctgataaaa
      541 atctaactcc ttacaaagct gaaactgtta atgatctgta ccttattgtt aaaaaattaa
      601 gccaaggtga tgtgaagaaa gcagttgtca gaattaaaga tggtggtcct agagattact
      661 atacttttga cttaactcgt cctttagaag agaacagaaa aaatattaaa gttgttaaaa
      721 acggtgaaat cgactcgatt tattgggatt aattaacata ctgaattaaa tagttgtacg
      781 ccaaacgtta gaaaacaatg ctaacgtatt ggcgtgcttt tttattttaa gtaacttcca
      841 atttatttag catctttacg actgtttaat aaagcacgta tgattaacac ggttgccact
      901 atatccgtta caatttttat gattgttaat acattcgatt tccttttcac aatagccacc
      961 tcgcttgttc aaaacataaa caactattgc attcactttt aagtaattat ttatatgatt
     1021 tatcccaata agctcaccat tcaaataaac acaatattat aaaataatta tacctttgga
     1081 tatagcaaaa agccacactc tatagcatgg cttctatcaa ttattttaaa gtattatatt
     1141 ttaaaactag atcgatttgt ctttgtaatt ttttcttttc atagctgtgt tggaaatgaa
     1201 ttaaattaac agctctttgt gctttacggt gtgttgcaac agttcttgta cgtttgaaca
     1261 agtttacagc tttttgtgca tccacaactt ttttatttac ttgtttttta aagtttgttt
     1321 gatactgatc atatttttta gcagcttcaa cgtttttagt ttgtgcatga gattcaccgg
     1381 caaacgttga taccgttaaa ctaatagcag ctatgcttaa aattgatttc ccaataaaat
     1441 tctttttcat
//
LOCUS       DAOBBH010000303         1437 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.305, whole genome shotgun sequence.
ACCESSION   DAOBBH010000303 DAOBBH010000000
VERSION     DAOBBH010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.305"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            256..1431
                     /gene="rpsA"
                     /locus_tag="P6L01_002603"
     CDS             256..1431
                     /gene="rpsA"
                     /locus_tag="P6L01_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDP3031632.1"
                     /translation="MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGII
                     PISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQ
                     EKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEE
                     LDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIHGKVARLTQFGAFIDIGGVDG
                     LVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQF
                     HENDVIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKIL
                     GIDEENERVSLSIKATLPNEDVVESDPSTTKAYLENEEEDNPTIGDMIGDKLKNLKL"
BASE COUNT          544 a          181 c          279 g          433 t
ORIGIN      
        1 atgacagact tataaaacaa aatagtaagc tatgctaatt tttatacaaa aaatatgtat
       61 aaacatagcg gactttatta cttgtttgaa tatatggttg taataaatat atgtccaact
      121 aatttacaga atgagattaa gtgacatatt tatggataca actaatttac taaatgtaat
      181 aaaggtagaa tttcttgaca attctgtcag tttataagat gttataaata tgtagtgtat
      241 aaggaggcaa acaagatgac tgaagaattc aatgaatcaa tgattaacga tattaaagaa
      301 ggtgacaaag tcactggcga ggtacaacaa gttgaagaca agcaagttgt tgttcatatc
      361 aacggtggta aatttaatgg gattattcct attagtcaac tatctacgca tcatattgat
      421 agcccaagtg aagttgtaaa agagggcgac gaagttgaag catatgtcac taaagttgag
      481 tttgatgaag aaaatgaaac tggagcttac atcttatcta gaagacaact tgaaactgag
      541 aagtcttata gttatttaca agaaaaatta gataataatg aaatcatcga agcgaaagta
      601 acagaagtag ttaaaggtgg tttggttgtt gatgtaggac aaagaggttt tgttccggct
      661 tcactaattt caacagactt cattgaggat ttctctgtgt ttgatggaca aacaattcgt
      721 attaaagttg aagaattgga tcctgaaaat aatagagtca ttttaagccg taaagcagtt
      781 gaacaagaag aaaacgatgc taaaaaagat caattattac aatctttaaa tgaaggcgat
      841 gttattcatg gtaaagtagc gcgtttaact caatttggtg catttataga cattggtggt
      901 gttgatggtt tagtgcatgt atctgaactt tctcacgaac atgttcaaac accagaagaa
      961 gtagtttcaa ttggtcaaga tgttaaagtt aaaattaaat ctattgatag agatacagaa
     1021 cgtatttcat tatcaatcaa agatacgtta ccaacacctt tcgaaaatat taaaggtcaa
     1081 ttccacgaaa atgatgtcat tgaaggtgtc gtagtaagat tggcaaactt tggtgcattt
     1141 gttgaaattg caccaggtgt acaaggactt gtacatattt ctgaaattgc acacaaacac
     1201 attggtacgc caggtgaagt gttagaacct ggtcaacaag taaatgttaa aatattaggt
     1261 attgatgaag agaatgaaag agtatcacta tctattaaag caacattacc aaacgaagat
     1321 gttgttgaaa gtgatccttc tacgactaag gcgtacttag aaaacgaaga agaagataat
     1381 ccaacaattg gcgatatgat tggtgataaa cttaaaaatc ttaaactata atttaat
//
LOCUS       DAOBBH010000304         1415 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.306, whole genome shotgun sequence.
ACCESSION   DAOBBH010000304 DAOBBH010000000
VERSION     DAOBBH010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.306"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     misc_feature    139..264
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            293..820
                     /gene="infC"
                     /locus_tag="P6L01_002604"
     CDS             293..820
                     /gene="infC"
                     /locus_tag="P6L01_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDP3031633.1"
                     /translation="MSTIAKDQTQINDKIRAKELRLIGQDGEQIGVKSKREALEMAER
                     VDLDLVVVAPNAKPPVARIMDYGKFKFEQQKKEKEMKKKQKIINVKEIRLSPTIEEHD
                     FQTKLKNGRKFLTKGDKCKVSIRFRGRAITHKEIGQRVLEKYADECKDIATVEQKPKM
                     DGRQMFIMLAPTAEK"
     gene            849..1049
                     /gene="rpmI"
                     /locus_tag="P6L01_002605"
     CDS             849..1049
                     /gene="rpmI"
                     /locus_tag="P6L01_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDP3031634.1"
                     /translation="MPKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKRQL
                     RKARLVSKSDMKRVKQLLAYKK"
     gene            1096..>1415
                     /gene="rplT"
                     /locus_tag="P6L01_002606"
     CDS             1096..>1415
                     /gene="rplT"
                     /locus_tag="P6L01_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDP3031635.1"
                     /translation="MPRVKGGTVTRARRKKTIKLAKGYFGSKHTLYKVAKQQVMKSGQ
                     YAFRDRRQRKRDFRKLWITRINAAARQHEMSYSRLMNGLKKAGIDINRKMLSEIAISD
                     EKAFA"
BASE COUNT          559 a          228 c          271 g          357 t
ORIGIN      
        1 aaaaattcca ttagaacaag tagatttacg tcaagacgtg tcaatggatg aaataaaagg
       61 ttaaaataaa aataattaca gttatagtgt tgacttaaat gtgaattgtt gatatgatta
      121 acaacgttga atacgaaacg aaccaagtag aagcacccgc ttctcacctg tagcgacgca
      181 taaagcagtt ggcaggttaa tcacattaca cataactgtg tataaggaag agcgggtatt
      241 ttatacctgc tcttcctttt tgcttggtgc aaatttcgta aaaattttgg aggtgtcaac
      301 catagcaaaa gatcaaactc aaatcaatga caaaattcgt gcaaaagaat tacgtttaat
      361 cggtcaagat ggtgaacaaa ttggtgttaa atcaaagcgt gaagctttag aaatggctga
      421 acgtgtagat ttagacttag tggtcgttgc accgaatgcg aaaccaccag ttgcaagaat
      481 tatggattac ggtaaattca aattcgaaca acagaaaaaa gaaaaagaaa tgaaaaagaa
      541 acaaaaaatt atcaatgtta aagaaattcg tttaagtcca acaattgagg aacatgattt
      601 ccaaactaag ttgaaaaacg gacgtaaatt cttaactaaa ggcgataaat gtaaagtatc
      661 tattcgtttc agagggcgtg ccattacgca taaggaaatt ggtcaacgtg tgctagaaaa
      721 atatgcagat gaatgcaaag atatagcaac agttgaacaa aaacctaaaa tggacgggcg
      781 tcaaatgttt atcatgttag cgccaacagc tgaaaaataa ttaaagctat tacaggagga
      841 cttatattat gccaaaaatg aaaactcacc gcggagcagc taaacgtgtt aaaagaactg
      901 cttcaggtca attaaaacgt tcaagagctt tcacatctca cttattcgca aacaagagca
      961 ctaaacaaaa acgtcaatta cgtaaagcta gattagtgtc taagagcgat atgaaacgtg
     1021 taaaacaatt attagcatac aaaaaataag aacaaataca gaaatcggta ggaattacct
     1081 aaggaggaat tttttatgcc acgagttaaa ggtggaacag taacaagagc gcgtcgtaaa
     1141 aaaacgatta aattagctaa aggttacttc ggttcaaaac atacattata caaagtagct
     1201 aagcaacaag taatgaaatc aggtcaatat gctttccgtg accgtcgtca acgtaaacgt
     1261 gacttccgta aattatggat tacacgtatc aacgcagcag ctcgtcaaca tgaaatgagc
     1321 tactcacgtt taatgaacgg tttgaaaaaa gctggtatcg acattaaccg taaaatgtta
     1381 tcagaaatcg caatttctga cgaaaaagca tttgc
//
LOCUS       DAOBBH010000305         1411 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.307, whole genome shotgun sequence.
ACCESSION   DAOBBH010000305 DAOBBH010000000
VERSION     DAOBBH010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.307"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            72..947
                     /locus_tag="P6L01_002607"
     CDS             72..947
                     /locus_tag="P6L01_002607"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDP3031636.1"
                     /translation="MFIIELIKGIILGVVEGLTEFAPVSSTGHMILVDDMWLKSSEFL
                     GSQSAFTFKIVIQLGSVFAAAWVFRERFLEILHIGKHKHVEGENDQQRRSKPRRLNLL
                     HVLVGMVPAGILGLLFDDFIEEHLFSVPTVMIGLFVGAIYMIIADKYSVKVKNPQTVD
                     QINYFQAFVIGISQAVAMWPGFSRSGSTISTGVLMKLNHKAASDFTFIMAVPIMLAAS
                     GLSLLKHYQDIQIADIPFYILGFLAAFTVGLIAIKTFLHLINKIKLIPFAIYRIVLVI
                     FIAILYFGFGIGKGI"
     gene            1119..>1411
                     /locus_tag="P6L01_002608"
     CDS             1119..>1411
                     /locus_tag="P6L01_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031637.1"
                     /translation="MDKKKVIKFMINVLPIVLVPLIVERKRIKQHPDVQKVTDATSKV
                     ASKTSAAISNTASDVKEYVGDKKQDFENKRELKKFAREHDPAYIEKKGEKLAK"
BASE COUNT          481 a          203 c          244 g          483 t
ORIGIN      
        1 gaatatgcta tcatgatatg gtaattttga tatttaaata aattaaattg agtaatgaaa
       61 gtgggtacat tatgtttatc attgaattaa taaaaggtat tatcttaggg gtcgtcgaag
      121 gattaacaga atttgcacct gtttcctcta ctggacatat gattctagtt gatgatatgt
      181 ggttaaaatc atctgaattt ttaggttctc aatcagcatt tacatttaaa atcgtcatcc
      241 aattaggatc cgtctttgca gcagcatggg tattccgcga acgcttctta gagattttac
      301 atattggtaa acacaaacat gttgaaggag aaaacgatca acaaagacgt tcaaagccaa
      361 gacgtttaaa tttattacat gtattagtgg gtatggtgcc agcaggtatt ttaggcttac
      421 tatttgatga tttcatcgaa gaacatttat ttagtgtgcc aactgttatg attggcttat
      481 tcgtaggtgc tatctatatg attattgctg ataagtattc agttaaagtt aaaaacccac
      541 aaacagtgga tcaaatcaat tatttccaag cgtttgtaat tggtatctct caagcagtag
      601 ctatgtggcc tggtttcagc cgttcaggct caacaatttc aactggggta ttaatgaaat
      661 taaatcataa agcagcatcg gacttcacat ttattatggc tgttccaatt atgttagcag
      721 ctagtggttt atctttatta aaacattatc aagatattca gattgctgat ataccttttt
      781 atattttagg ctttttagct gcatttactg ttggactaat tgcaatcaaa acattcttgc
      841 atctaattaa caaaatcaaa ctaattccat ttgccattta cagaattgta ttagtaatct
      901 ttattgctat actttacttc ggatttggca ttggtaaagg tatttaattt ccttttaaaa
      961 tatagactgt caattaagga accacttgat tggcagtttt tattgtcttc ttttaaaaaa
     1021 catctgaaat acttaatagt gattcactac tatcatcgtt tatattgatg tatcataggg
     1081 tatgtaaaat taataaagta attatggagg caagatctat ggataagaaa aaagtcatca
     1141 aatttatgat taatgtatta ccaattgtat tggtaccgtt aattgttgaa cgtaaacgta
     1201 tcaaacaaca tccggacgta caaaaagtta cagatgctac aagtaaagtt gcttcaaaaa
     1261 catctgcagc aatcagtaac acagcgagtg atgttaaaga atatgtcggc gataaaaaac
     1321 aagattttga aaataaacgt gaacttaaaa agtttgctag agaacatgat cctgcctata
     1381 ttgagaaaaa aggcgaaaaa ttagctaaac a
//
LOCUS       DAOBBH010000306         1411 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.308, whole genome shotgun sequence.
ACCESSION   DAOBBH010000306 DAOBBH010000000
VERSION     DAOBBH010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.308"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            59..337
                     /locus_tag="P6L01_002609"
     CDS             59..337
                     /locus_tag="P6L01_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031638.1"
                     /translation="MKKSQKYEEKAGLTPNKLDEKTEKKGDKLAEKNRKEIAKMNKKL
                     QKNIEKRHKEEQKRQQEADKARIKSFKKYKDYVAKSASQQNKENNTEA"
     gene            340..798
                     /locus_tag="P6L01_002610"
     CDS             340..798
                     /locus_tag="P6L01_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDP3031639.1"
                     /translation="MTHIIIDGDACPVVDSIIDLTTETGIFVTIIRSFSHFSNQLYPP
                     HVSTLYVDDGPDAVDYKIVQLSTKDDIVVTQDYGLASLLVDKVLIVMHHNGKIYNSKN
                     IQQLLDKRYMNAQIRKQGGRHKGPPPFTKQDQKVFEQSLLKVIHRIKELD"
     gene            800..1356
                     /locus_tag="P6L01_002611"
                     /pseudo
     CDS             800..1356
                     /locus_tag="P6L01_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
BASE COUNT          550 a          228 c          249 g          384 t
ORIGIN      
        1 gcgtcataaa gaagagcaaa aagcccgcga aaagaatgaa atacaacgta ttaaagatat
       61 gaaaaaatca caaaaatacg aagaaaaagc aggcttaaca cctaataaat tagatgagaa
      121 aactgagaaa aaaggcgata aactagctga aaaaaatcgc aaagaaatcg ctaaaatgaa
      181 taaaaagtta caaaaaaata ttgaaaaacg acacaaagaa gaacaaaaac gccaacaaga
      241 agctgataaa gcacgcatca agtcatttaa aaaatataaa gattatgttg ccaaaagcgc
      301 ctctcaacaa aataaagaaa acaatacaga ggcataattg tgacacatat tattattgat
      361 ggagatgctt gtcctgttgt tgattctatt atagatttaa caactgagac aggcattttt
      421 gtgacaatta ttcggagctt cagccatttt tcgaaccaat tatatcctcc acatgtatca
      481 acattatatg ttgatgatgg accagatgca gttgattaca aaattgttca attatcaacg
      541 aaggatgata tcgtagtcac tcaagattat ggactcgcaa gtttattagt cgacaaagtc
      601 ttaattgtca tgcatcataa tgggaagata tacaattcca aaaatattca acaactatta
      661 gataaacgat atatgaatgc acaaattaga aaacaaggtg gtcgccacaa aggtccccca
      721 ccttttacaa agcaagatca aaaagtgttt gaacaatcac tattaaaagt cattcatcga
      781 attaaggagc tggattaaca tgaaacgaat cgcagtttat tgtggtgcaa gcaaaggtca
      841 tgacccttca tatgtacaaa aagcatacga tttaggtaaa tattttgctg aacaaggtta
      901 tgaattagta tttggtgcag ggtcaattgg cattatgggt gcggtgtatt aaatcatggc
      961 ggcaaagcta ttggcgtcat gcctaaaatg ttagatgaac atgaaatcac aagccaacgt
     1021 ttaactgaat taattttagt tgattctatg catgaacgta aaaataaaat ggcagaactt
     1081 gcagatgcat ttgtcatggc tcctggtggc gcaggttcac tagaagaatt tttcgaaatg
     1141 tatagttggg ctcaaatagg tatccatgaa aagcctattg ccatttataa tattaatggc
     1201 tttttcaatc cactacaaac aatgattgac catatgattg aggaaggttt tattgatcct
     1261 aagtatcgtg ctcttgcacc gttatgcgac actaaggaat cattaataga atcaatatta
     1321 aatttcaaac ctctgggtac tcgttcatac gattaatcat tctaaggtta aaaaataaac
     1381 aaacacccct aatattgtta tcatcagcta a
//
LOCUS       DAOBBH010000307         1403 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.309, whole genome shotgun sequence.
ACCESSION   DAOBBH010000307 DAOBBH010000000
VERSION     DAOBBH010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.309"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(20..93)
                     /locus_tag="P6L01_002612"
     tRNA            complement(20..93)
                     /locus_tag="P6L01_002612"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(57..59),aa:Met,seq:cat)
     gene            complement(108..200)
                     /locus_tag="P6L01_002613"
     tRNA            complement(108..200)
                     /locus_tag="P6L01_002613"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(162..164),aa:Ser,seq:tga)
     gene            complement(239..314)
                     /locus_tag="P6L01_002614"
     tRNA            complement(239..314)
                     /locus_tag="P6L01_002614"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(279..281),aa:Asp,seq:gtc)
     gene            complement(325..414)
                     /locus_tag="P6L01_002615"
     tRNA            complement(325..414)
                     /locus_tag="P6L01_002615"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(376..378),aa:Ser,seq:tga)
     gene            complement(424..497)
                     /locus_tag="P6L01_002616"
     tRNA            complement(424..497)
                     /locus_tag="P6L01_002616"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(461..463),aa:Ile,seq:cat)
     gene            complement(526..599)
                     /locus_tag="P6L01_002617"
     tRNA            complement(526..599)
                     /locus_tag="P6L01_002617"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(563..565),aa:Met,seq:cat)
     gene            complement(621..696)
                     /locus_tag="P6L01_002618"
     tRNA            complement(621..696)
                     /locus_tag="P6L01_002618"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(661..663),aa:Ala,seq:tgc)
     gene            complement(714..787)
                     /locus_tag="P6L01_002619"
     tRNA            complement(714..787)
                     /locus_tag="P6L01_002619"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(751..753),aa:Pro,seq:tgg)
     gene            complement(797..870)
                     /locus_tag="P6L01_002620"
     tRNA            complement(797..870)
                     /locus_tag="P6L01_002620"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(834..836),aa:Arg,seq:acg)
     gene            complement(889..977)
                     /locus_tag="P6L01_002621"
     tRNA            complement(889..977)
                     /locus_tag="P6L01_002621"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(941..943),aa:Leu,seq:taa)
     gene            complement(988..1062)
                     /locus_tag="P6L01_002622"
     tRNA            complement(988..1062)
                     /locus_tag="P6L01_002622"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1028..1030),aa:Gly,seq:gcc)
     gene            complement(1066..1147)
                     /locus_tag="P6L01_002623"
     tRNA            complement(1066..1147)
                     /locus_tag="P6L01_002623"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1111..1113),aa:Leu,seq:tag)
     gene            complement(1151..1226)
                     /locus_tag="P6L01_002624"
     tRNA            complement(1151..1226)
                     /locus_tag="P6L01_002624"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1191..1193),aa:Lys,seq:ttt)
     gene            complement(1232..1307)
                     /locus_tag="P6L01_002625"
     tRNA            complement(1232..1307)
                     /locus_tag="P6L01_002625"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1272..1274),aa:Thr,seq:tgt)
     gene            complement(1324..1399)
                     /locus_tag="P6L01_002626"
     tRNA            complement(1324..1399)
                     /locus_tag="P6L01_002626"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1364..1366),aa:Val,seq:tac)
BASE COUNT          373 a          379 c          336 g          315 t
ORIGIN      
        1 ttaaattaaa aactatgtat tgcgggaggc ggatttgaac caccgacctt cgggttatga
       61 gcccgacgag ctaccgaact gctccatccc gcgataataa aaaataatgg cggaggaaga
      121 gggattcgaa cccccgcggc ccgttaaggc cctgtcggtt ttcaagaccg atcccttcag
      181 ccggacttgg gtattcctcc attattatag gtaaatcgct attaattata aaattaaatg
      241 gcggtctcga cgggaatcga acccgcgatc tcctgcgtga caggcaggcg tgttaaccgc
      301 tacactacga gaccattagt aaaacggagg aagagggatt cgaacccccg cgagccgtta
      361 agcccctgtc ggttttcaag accgatccct tcagccggac ttgggtattc ctccaaaatt
      421 atatggacct tgcaggactc gaacctgcga ccgaacggtt atgagccgtt agctctaacc
      481 aactgagcta aaggtcctaa atataatttt acaactaata aatagtggcg gtggagggga
      541 tcgaaccccc gacctcacgg gtatgaaccg tacgctctag ccagctgagc tacaccgcct
      601 tatatagttt gtaaataata tggtggagac tagcgggatc gaaccgctga cctcctgcgt
      661 gcaaagcagg cgctctccca gctgagctaa gcccccataa taattacagt atatcgggaa
      721 gacaggattc gaacctgcga ccccttggtc ccaaaccaag tgctctacca agctgagcta
      781 cttcccgtat aattaacgcg cccgatagga gtcgaaccca taacctcttg atccgtagtc
      841 aaacgctcta tccaattgag ctacgggcgc atatgttttt attgaaaatg gtgccgagga
      901 ccggaatcga accggtacgg tgatcactca ccgcaggatt ttaagtcctg tgcgtctgcc
      961 agttccgcca ccccggcact ataaaaatgg agcagaagac gggattcgaa cccgcgaccc
     1021 caaccttggc aaggttgtat tctaccgctg aactacttct gcatatgcgg gtgaagggag
     1081 tcgaaccccc acgccgtaag gcgctagatc ctaagtctag tgcgtctgcc aattccgcca
     1141 cacccgcaaa tggtgagcca tagaggattc gaacctctga ccctctgatt aaaagtcaga
     1201 tgctctacca actgagctaa tggctcttcc atggtgccgg ccagaggact tgaaccccca
     1261 acctactgat tacaagtcag ttgctctacc aattgagcta ggccggcaat atgtaagaat
     1321 aaatggtgga gaatgacggg ttcgaaccgc cgaccctctg cttgtaaggc agatgctctc
     1381 ccagctgagc taattctccg att
//
LOCUS       DAOBBH010000308         1398 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.310, whole genome shotgun sequence.
ACCESSION   DAOBBH010000308 DAOBBH010000000
VERSION     DAOBBH010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.310"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..568
                     /locus_tag="P6L01_002627"
     CDS             <1..568
                     /locus_tag="P6L01_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031640.1"
                     /translation="INIAIVVLITGFNLYRHQMQHLSLSAMLLSITINAFINTFIIDK
                     YNFITLCTITMFIIWTILQFYIDKKLKPVYITDQKFIAIILTIVVSLTQRVTDFSSTQ
                     SIYMSIPFLAPAIFIIGGIMLFISTFNSLDETAENNNKIKKLMIKGLIIINISFIVMM
                     VLTPYWYLYLIVYLIFLLFLLWQKVYKF"
     gene            848..1288
                     /locus_tag="P6L01_002628"
     CDS             848..1288
                     /locus_tag="P6L01_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HDP3031641.1"
                     /translation="MNTKYFLAVGAVASVLTLGACGNSNSQDQGNKTEQKTKSEDSNV
                     KTDKTKHLTGTFSSKNGETVEGKAEIKNGKLMLTNYKSSKGPDLYVYLTKNGDIKNGK
                     EIAMVDYDKEKQTFDLKNVDLSKYDEVTIYCKKAHVIFGGAKLK"
BASE COUNT          522 a          207 c          178 g          491 t
ORIGIN      
        1 aattaatatt gctatcgtcg tacttataac tggattcaat ttatataggc atcaaatgca
       61 acatttatca ttaagtgcaa tgttgttatc tattacgata aacgccttta taaatacatt
      121 cataattgat aaatataact ttattactct gtgtactata acgatgttta tcatttggac
      181 gatactacag ttttacattg ataaaaaatt aaaacctgta tacattacag accaaaaatt
      241 tattgctatc atattaacga ttgttgtcag tttaacgcaa cgtgttaccg acttttcaag
      301 tacacaatca atatatatgt ctattccatt tttagcccct gcaattttca tcattggtgg
      361 tattatgttg tttatttcaa catttaatag cttagatgaa actgctgaaa ataataacaa
      421 aataaagaaa ctaatgatta aaggacttat cattattaac atttcattta tcgttatgat
      481 ggttttaaca ccatattggt acttgtattt aatcgtctat cttattttct tgttgttttt
      541 attgtggcaa aaggtttata aattttaata ccaaaactat aaaacacttc tgatattctt
      601 agttcaaaat atcagaagtg ttttatagtg ttatctagtt cagataaata ttttctcacc
      661 taaaaaaatg ccctcctctt attttgactc ctatttattt aaatcagaca attattttca
      721 ttttcaaatt attctttctt caatattagt taagcgatat ttaaacgaag ttaagaatta
      781 gttaatggca tattatttgc cttcatttta aacttaactt atcaaattga agaaatgagg
      841 agttagcatg aatacaaaat attttttagc agttggtgct gttgcctctg tattaacttt
      901 aggcgcttgt ggtaattcta attcacaaga tcaaggtaac aaaactgaac aaaaaacaaa
      961 gtcagaagat agcaatgtta aaactgataa aacaaaacac ctaacaggta cattcagttc
     1021 taaaaacggt gaaactgttg aaggtaaagc tgagattaaa aatggtaaat taatgcttac
     1081 taactacaaa tcatcaaaag gtccagattt atacgtctac ctaacaaaaa atggcgacat
     1141 taaaaacggt aaagaaatcg caatggttga ctacgataaa gaaaaacaaa catttgatct
     1201 taaaaatgta gatttaagca aatatgatga agtaacaatc tattgtaaaa aagctcacgt
     1261 catcttcggc ggcgctaaat taaaataatc aatttctgag ttaaactttt atatacaaca
     1321 tactattact atacattaca aattttaaaa atatacatta cactcattac tcaatggaaa
     1381 gcgtatgatt tcacagcc
//
LOCUS       DAOBBH010000309         1385 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.311, whole genome shotgun sequence.
ACCESSION   DAOBBH010000309 DAOBBH010000000
VERSION     DAOBBH010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.311"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..219
                     /locus_tag="P6L01_002629"
     CDS             <1..219
                     /locus_tag="P6L01_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031642.1"
                     /translation="NGSDHANPPNNERIPTPHIHIYTEEYNNGGIAIPLKDIEDLELT
                     DEIIESLDFFMKYTNIKHDNVIKEPRLL"
     gene            240..1016
                     /locus_tag="P6L01_002630"
     CDS             240..1016
                     /locus_tag="P6L01_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
                     /protein_id="HDP3031643.1"
                     /translation="MKTIEERMNEYFNWLKQNYIFKELDSSTEITTPFKNHLNDFIRI
                     YADTLPNNEICLSDDGLTINELEMLGIDINTKTRTKLIQNILNQFNLKLVDKEITADV
                     KNESFAQSKHNLIQGILKIYDLTLTTKSNVTNIFYEEVFEFLYDQKIRGLAQVSVSGE
                     SGLKYSIDYIVSETKSQPEKLVNFTNNLDFNKITNEAYIYRDIKPNRPSRNKLEPIML
                     IIVNDVDHPINERAQTVAEHENLEILKWSNKSKIIETLTS"
BASE COUNT          573 a          192 c          188 g          432 t
ORIGIN      
        1 aatggtagtg atcatgcaaa tccaccaaat aatgaaagaa tacctacgcc acatatacac
       61 atatatactg aagaatataa taatggtggt attgcgatcc cattaaaaga tattgaagat
      121 ttagaattaa cagatgaaat tatagaatca ttagacttct ttatgaagta tactaatata
      181 aaacacgata atgttataaa agaaccaaga ttactgtaaa agaaatggag ggatacttag
      241 tgaagactat cgaagaaaga atgaacgaat atttcaactg gttaaagcaa aattatatct
      301 tcaaagaatt agatagttct acagaaatta ctactccctt taagaaccat ttgaatgatt
      361 ttattagaat atatgctgat acactaccta ataatgaaat ttgtctatca gatgacggat
      421 tgacgattaa tgaattagaa atgttgggta ttgatataaa cacaaaaacg agaactaaac
      481 ttatacagaa tattttaaat caatttaatt taaaattagt tgataaagaa attacagcgg
      541 atgttaaaaa tgagagtttt gctcagtcta aacataattt aattcaaggc atattgaaaa
      601 tttatgacct tactttaact actaaatcta acgttacaaa catattctat gaagaagtat
      661 ttgaattctt atatgaccaa aaaataagag gtttagcaca agtttctgtt tctggagaat
      721 caggtttaaa atattctatt gattatattg tttctgaaac aaaatcacaa ccagaaaaac
      781 tagttaactt tacaaacaac ttagatttca acaaaataac taatgaagct tatatttacc
      841 gtgatatcaa accaaacaga ccatccagaa acaaattaga acctatcatg ttgataattg
      901 taaatgatgt agatcatccg ataaatgaaa gagcacaaac tgtagcagaa catgaaaacc
      961 ttgagatatt aaaatggtct aataaatcaa aaatcattga aactttaacc tcataaatta
     1021 gcagtagtcc cttatttata ttctttaatt tccaaaccat atcgtgcaaa tagaaaagca
     1081 gataactttt ttatttgcac gttttatttt agaacaagaa agaacaaggt caacgagtca
     1141 gaaaaacttt ttcttgatac gtatttgaca cttgctcttt taagaaaact aacaattcat
     1201 tgtatttccg ttaaattaac ggagaattcg gattacctag atagatttac ctctagagtg
     1261 ttaaaaaatt gtacattctt aaacaatcat aaatgtacgt tattaaatta tcatttccac
     1321 tggagaatca tgtcgactaa aagattataa aagtaaaaat acatttaaaa gcacgtcatt
     1381 ttcaa
//
LOCUS       DAOBBH010000310         1383 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.312, whole genome shotgun sequence.
ACCESSION   DAOBBH010000310 DAOBBH010000000
VERSION     DAOBBH010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.312"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(1..498)
                     /gene="efb"
                     /locus_tag="P6L01_002631"
     CDS             complement(1..498)
                     /gene="efb"
                     /locus_tag="P6L01_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Efb"
                     /protein_id="HDP3031644.1"
                     /translation="MKNKLIAKSLLTIAAIGITTTTIASTADASEGYGPREKKPVSIN
                     HNIVEYNDGTFKYQSRPKFNSTPKYIKFKHDYNILEFNDGTFEYGARPQFNKPAAKTD
                     ATIKKEQKLIQAQNLVREFEKTHTVSAHRKAQKAVNLVSFEYKVKKMVLQERIDNVLK
                     QGLVR"
     gene            complement(758..1264)
                     /locus_tag="P6L01_002632"
     CDS             complement(758..1264)
                     /locus_tag="P6L01_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031645.1"
                     /translation="MIIKILTILLLLCILSYLVTNRKKPFLFLKTLFMGVVFIFIGYI
                     SLAISAVIIYGIIQFITIDFGSFFLMGIILILISSIFQLFIVRLLFRKKNVDLTEVVV
                     LEHLIQWFLVYFAIYQAVNEKMDINDINIDNFQSVFFDVSNLNLVILPTLIISWVTIF
                     NYRMRSYK"
BASE COUNT          474 a          223 c          168 g          518 t
ORIGIN      
        1 ttatctcact aatccttgtt ttaatacatt atcaattcgc tcttgtaaga ccattttctt
       61 cactttgtat tcaaacgaaa ctaagttgac tgccttttgt gcttttctgt gtgcactgac
      121 agtatgtgtt ttttcaaatt ctctcacaag attttgagct tgaatcaatt tttgttcttt
      181 tttaatagtt gcatcagttt tcgctgctgg tttattaaat tgtggacgtg caccatattc
      241 gaatgtacca tcgttaaatt ctaaaatatt atagtcatgt ttgaatttaa tatatttagg
      301 tgttgagtta aattttggtc tagattgata tttaaaagta ccatcattgt actctacgat
      361 attgtgatta atactcactg gtttcttttc tcttggaccg tatccttcgc tcgcatctgc
      421 tgttgacgca attgtagttg tagtaatacc aattgccgct attgttaata aagattttgc
      481 tatcaattta tttttcatgt taattatcct ccaaattatt atcttacatt tattaagtta
      541 tcctatttat acaacttttg aaattaacta attattaatt aactataaac actttataat
      601 atagacaaaa tatagttatc tatcttgtga ctcatcagca cttatacact agtcaaaaaa
      661 ttcacgcctc tactttttcg aatttcaaaa ataaaaccac acaacaaaaa aagacaccat
      721 ttgaatgttg gtgtctgatg gtataatttc atagatttta tttgtaactt ctcattctat
      781 agttaaatat tgtgacccag ctaatgatta acgttggtag aattactaaa ttcaaattag
      841 acacgtcaaa aaagacagat tggaaattgt cgatattaat atcattaatg tccatttttt
      901 catttactgc ttgatagatc gcaaagtaaa ctaagaacca ttgaattaaa tgctctaaaa
      961 cgacaacctc tgtcaaatcg acattctttt ttctaaaaag taatctaact ataaataatt
     1021 ggaatatact tgaaatcaag atcaatataa tacccattaa gaaaaaacta ccaaaatcaa
     1081 ttgtgataaa ttgaataata ccataaataa ttacggcaga tattgccagt gaaatatatc
     1141 ctataaagat aaataccaca cccataaaga gtgtctttaa gaacagaaaa ggcttctttc
     1201 tatttgtaac caaatagctc aatatacaaa gtagtaatag aattgttaaa attttaatta
     1261 tcatcgtttg acctcaatat atttatttat atctttatta ttgaccacga aaaataacaa
     1321 tcacttaagt gattgccaat aatactcaat gcctactcta tagtaatctt ggcacgtgat
     1381 ttt
//
LOCUS       DAOBBH010000311         1382 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.313, whole genome shotgun sequence.
ACCESSION   DAOBBH010000311 DAOBBH010000000
VERSION     DAOBBH010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.313"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            340..1299
                     /gene="hyl"
                     /locus_tag="P6L01_002633"
     CDS             340..1299
                     /gene="hyl"
                     /locus_tag="P6L01_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin"
                     /protein_id="HDP3031646.1"
                     /translation="MKTRIVSSVTTTLLLGSILMNPVANAADSDINIKTGTTDIGSNT
                     TVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKS
                     GLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGG
                     LIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYG
                     NQLFMKTRNGSMKAAENFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYE
                     RVRDDYQLHWTSTNWKGTNTKDKWTDRSSERYKIDWEKEEMTN"
BASE COUNT          532 a          205 c          217 g          428 t
ORIGIN      
        1 ttatattgta atcgattaca tttttaaatc aaacaaatca ttagttttaa agaaattaat
       61 caataaaatt agctatgtct tttccttgtt tcataaagaa tttattcaac tttgactaac
      121 cctcgaaatt gaaatgcttc ctttcaaatt ttaaataaaa gttaaaaaca tattcttaaa
      181 ataatcactc atcatcactc agtaatttat cagttgctac attaaatata ctaatataaa
      241 cgattataaa tatttgatat gtctcaactg caatattcta aattgacata ttgattattg
      301 tttcctcaaa tcgttaaaaa aaatagaagg atgatgaaaa tgaaaacacg tatagtcagc
      361 tcagtaacaa caacactatt gctaggttcc atattaatga atcctgtcgc taatgccgca
      421 gattctgata ttaatattaa aaccggtact acagatattg gaagcaatac tacagtaaaa
      481 acaggtgatt tagtcactta tgataaagaa aatggcatgc acaaaaaagt attttatagt
      541 tttatcgatg ataaaaatca caataaaaaa ctgctagtta ttagaacgaa aggtaccatt
      601 gctggtcaat atagagttta tagcgaagaa ggtgctaaca aaagtggttt agcctggcct
      661 tcagccttta aggtacagtt gcaactacct gataatgaag tagctcaaat atctgattac
      721 tatccaagaa attcgattga tacaaaagag tatatgagta ctttaactta tggattcaac
      781 ggtaatgtta ctggtgatga tacaggaaaa attggcggcc ttattggtgc aaatgtttcg
      841 attggtcata cactgaaata tgttcaacct gatttcaaaa caattttaga gagcccaact
      901 gataaaaaag taggctggaa agtgatattt aacaatatgg tgaatcaaaa ttggggacca
      961 tatgatagag attcttggaa cccggtatat ggcaatcaac ttttcatgaa aactagaaat
     1021 ggttctatga aagcagcaga gaacttcctt gatcctaaca aagcaagttc tctattatct
     1081 tcagggtttt caccagactt cgctacagtt attactatgg atagaaaagc atccaaacaa
     1141 caaacaaata tagatgtaat atacgaacga gttcgtgatg actaccaatt gcattggact
     1201 tcaacaaatt ggaaaggtac caatactaaa gataaatgga cagatcgttc ttcagaaaga
     1261 tataaaatcg attgggaaaa agaagaaatg acaaattaat gtaaattatt tgttcatgta
     1321 caaataaata taatttataa ctttagccga taacttcaga aatgatgttt ttcggctaat
     1381 tt
//
LOCUS       DAOBBH010000312         1382 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.314, whole genome shotgun sequence.
ACCESSION   DAOBBH010000312 DAOBBH010000000
VERSION     DAOBBH010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.314"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(262..1305)
                     /locus_tag="P6L01_002634"
     CDS             complement(262..1305)
                     /locus_tag="P6L01_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDP3031647.1"
                     /translation="MNKDNKWTMITALFITVISVLLAFHLKQHYDQITNENHANKDKI
                     NIKNKNVRIYQNLTYNRVFPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKN
                     PLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVIIGG
                     DSAGAQLASQFTAIQTNDRLREAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRI
                     QLFMKSYTGEEDWEKSFKNISQMSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQ
                     ELNVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRNTSSSGIQTEEKPQM
                     ENPSNELPLNPLN"
BASE COUNT          440 a          216 c          200 g          526 t
ORIGIN      
        1 ttttcaattt ataaatttat tatatcatgt acacactgtt tttcgacaat ttagttgtaa
       61 aaagttattt tcaggttcaa aacaaattag tttgtaaaat aaatgtcaca tcataatagg
      121 gtgagataga aatgataaag aaccgctaac aatttattat atctcagact cttacacatt
      181 agccccacat gttttaaatt cttcgttttc aatttgaaga tcttaaatgt tgcttaagta
      241 ataaattact actgtttatc attagtttaa aggatttaac ggtaattcat tactcggatt
      301 ttccatttgt ggtttctctt cagtttgaat accactagaa gatgtattac gacttaagaa
      361 aagtaacact tttttgatgt tatctatcga ttcaggttta ttaaggtgaa attgatactg
      421 atgatgtaaa tgatgcgtac catcataaaa caaagtatct actggtacat tcaattcttg
      481 taatttctta ctgaattcta tattttgact ttcgaatgga tcgctatctc caacagataa
      541 aaatgttggt ggataatttt ttgtcgattg ttttactgtc gacatttgtg aaatattttt
      601 aaagctcttt tcccaatctt cttcgccagt ataacttttc ataaataact gtattcttgg
      661 aaactcagtt tctctaactg tttgcatatt ataaaaacca ccaaatagta tagcaccttt
      721 aatttgcgat ggtttgaatg actgatcaaa tttcatggct tctcttaagc gatcattcgt
      781 ctgtattgcc gtaaattggc tagctaattg agcacctgca gaatcaccgc caataattac
      841 ttgattaaaa tcaataggta aattcatttt attttcttta atgaattgag ttgcttgatt
      901 catttgaatt aatggtgtgg gatatttata ttgtggcgcc aatgcataat ttacattcac
      961 aacaatgtac ccttgttcag caattttcgc taataatggg tttttatact gcttatcacc
     1021 cgcaatataa ccaccaccgt gcatccaaaa aataactggc agtttggcat tagaagacat
     1081 atcaacaggt gtaataatat ctaatttact gttagggaaa actctattgt atgtaaggtt
     1141 ttgataaatg cgcacatttt tatttttaat attaattttg tctttattag catggttctc
     1201 atttgtaatt tggtcataat gttgtttcag atgaaatgct aacaatacac tgattacagt
     1261 tataaaaagc gcagttatca tcgtccattt attatcttta ttcattacat cgtacctctc
     1321 tatttgttag acgctcatat taatagactg aataaatata aaaagcaatt cgcccgataa
     1381 ga
//
LOCUS       DAOBBH010000313         1367 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.315, whole genome shotgun sequence.
ACCESSION   DAOBBH010000313 DAOBBH010000000
VERSION     DAOBBH010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.315"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..1119
                     /gene="scpA"
                     /locus_tag="P6L01_002635"
     CDS             <1..1119
                     /gene="scpA"
                     /locus_tag="P6L01_002635"
                     /EC_number="3.4.22.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain A"
                     /protein_id="HDP3031648.1"
                     /translation="LSVTPIANAESNSNIKAKDKKHVQVNVEDKSVPTDVRNLAQKDY
                     LSYVTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVLNTEGNIDYIVTISPKI
                     TKYSSSSSKYTINVSPFLSKVLNQYKDQQITILTNSKGYYVVTQNHKAKLVLKTPRLE
                     DKKLKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQYINKLENFKIRETQGN
                     NGWCAGYTMSALLNATYNTNKYHAEAVMRFLHPNLQGQRFQFTGLTPREMIYFGQTQG
                     RSPQLLNRMTTYNEVDNLTKNNKGIAVLGSRVESRNGMHAGHAMAVVGNAKLDNGQEV
                     IIIWNPWDNGFMTQDAKNNVIPVSNGDHYRWYSSIYGY"
     gene            1150..>1367
                     /locus_tag="P6L01_002636"
     CDS             1150..>1367
                     /locus_tag="P6L01_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin A"
                     /protein_id="HDP3031649.1"
                     /translation="MEQIELFSIDKFKCNSEAKYYLNIIEGEWHPQDLNDSPLKFILS
                     TSDDSDYICKYINTEHKQLTLYNKNNSS"
BASE COUNT          535 a          203 c          214 g          415 t
ORIGIN      
        1 ttaagtgtta cccctattgc aaacgctgag agcaattcaa atatcaaagc aaaagataag
       61 aagcatgtgc aagttaatgt cgaggacaag agtgttccta ctgacgtacg taatttagcg
      121 caaaaagact atctttctta tgttacttca cttgataaaa tctataataa agagaaagcc
      181 tcgtatacac ttggtgaacc ttttaaaatt tataaattca acaaaaagag tgatggtaat
      241 tattattttc cagttttgaa cactgaaggt aatatcgatt atatagttac aatttcacca
      301 aaaatcacta agtattctag ttcatcttct aaatacacaa tcaatgtttc tccattttta
      361 tctaaagtct taaatcaata taaagatcaa caaattacta ttttgacaaa ctcaaaaggt
      421 tactatgtgg ttactcaaaa tcataaagct aaactagtac taaaaacacc acgtctagaa
      481 gataaaaagt taaaaaagac tgagagtata cctactggca ataatgtaac gcaactaaag
      541 caaaaagctt ctgtcactat gccaactagt caatttaaaa gtaataatta tacttacaac
      601 gagcaatata taaataaact tgagaatttc aagataagag aaactcaagg taacaatggt
      661 tggtgtgcag gctatacgat gtctgcatta ctaaatgcga cttataatac taacaaatat
      721 catgcagaag ctgtgatgag atttttacac ccaaacttac aaggacaacg attccaattt
      781 actggtttaa cgccaagaga gatgatttat tttggacaaa ctcagggaag aagtccacaa
      841 cttcttaata gaatgactac atataatgaa gttgacaatc ttactaaaaa taataaagga
      901 attgctgttt taggtagtag agtagaatca agaaatggta tgcacgcagg gcatgccatg
      961 gcagttgttg ggaacgctaa attagataat ggacaagagg ttataattat atggaatcca
     1021 tgggataatg gttttatgac tcaagatgct aaaaacaatg ttattccagt atccaatggc
     1081 gaccattatc gatggtattc atctatttat ggttattaaa atttgtattt tagaaaggta
     1141 aataatatta tggaacaaat tgaattattt agtattgata aattcaaatg taattcagag
     1201 gctaagtatt atcttaatat tattgaggga gaatggcatc ctcaagattt aaatgatagt
     1261 cctttaaaat ttattctcag tacctcagac gattctgatt acatttgcaa atatataaat
     1321 acagaacaca aacaactcac attatataat aaaaataata gctcaat
//
LOCUS       DAOBBH010000314         1342 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.316, whole genome shotgun sequence.
ACCESSION   DAOBBH010000314 DAOBBH010000000
VERSION     DAOBBH010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.316"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(23..961)
                     /locus_tag="P6L01_002637"
     CDS             complement(23..961)
                     /locus_tag="P6L01_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDP3031650.1"
                     /translation="MKQFVNLGKSDVEVFPIALGTNAVGGHNLYPNLDEEQGKDVVRQ
                     AINHGINLLDTAYIYGPERSEELVGEVVKEYPREQIKIATKGSHEFDENQEVHQNNQP
                     EYLKQQVENSLKRLQTDYIDLYYIHFPDDNTPKDQAVAALQELKEQGKIKAIGVSNFT
                     LDQLKEANKDGYVDVVQLEYNLLHRENEAVLQYCVDHLITFIPYFPLASGILAGKYDE
                     NTKFSDHRTTRRDFIPGVFEENVRRVKALESLAAAHQTSIANIVLAFYLTRPAIDVII
                     PGAKRAEQVIENIKAADIVLSDDEIQYIDELFPIED"
BASE COUNT          430 a          241 c          194 g          477 t
ORIGIN      
        1 atcaccatta aatacaattc atttagtctt caattggaaa caattcatcg atatattgaa
       61 tctcatcatc tgataaaacg atatctgcag ctttaatatt ttcaatgact tgttctgcac
      121 gttttgcacc aggaataatc acatcgatag ctggtctcgt taaataaaat gctaatacaa
      181 tgttcgcaat tgaagtttga tgtgctgcag ctaggctttc caaagctttt acgcgacgca
      241 cattctcttc aaaaacacca ggtataaaat cacgacgtgt agtacgatgg tcactaaatt
      301 tagtgttctc atcatatttt ccagctaaaa taccggatgc taatgggaaa taaggaataa
      361 atgtgattag gtgatcaaca caatattgca atactgcctc attttcgcgg tgcaataaat
      421 tatattctaa ctgtacaaca tcaacgtaac catctttatt tgcttcttta agttgatcta
      481 atgtgaaatt tgatacacca atggctttaa tcttcccttg ttccttaagc tcttgtaatg
      541 ctgcaactgc ttgatctttc ggagtgttgt catcaggaaa atgaatataa tataaatcga
      601 tataatcagt ttgtagacgt ttcaaactat tctcaacttg ttgttttaaa tattccggtt
      661 gattgttctg atgtacttct tgattttcat caaattcatg agaccctttc gtagcaattt
      721 taatttgctc tcgcggatat tccttaacaa cttctccaac caattcttct gatcgttctg
      781 gcccataaat atatgccgta tctaataaat taataccatg attaatggct tgacgaacaa
      841 catcttttcc ttgttcttca tctaagttcg gatataaatt atgcccacct actgcgttcg
      901 tcccaagtgc gattggaaac acttcaacat cagatttacc taagtttaca aattgcttca
      961 ttagacccag ctccttatct ttataacttt tccactatca cactatcatg atttaataca
     1021 acttccaaat gttagaatta gggtttgaat taaggcggtg tattagaaaa atgttatgta
     1081 gaactcagtt aaaataagta ttgcttaagt tgatatgcat atagcgcgat tgctcgttga
     1141 cctttaaatt acatgtactt ttgaatcaga aattgaaaaa catttaagaa cgacaatatt
     1201 aaagccattg caaatttaat tagaaatact ataatgtttt gatttgcata acaaagaaat
     1261 taaaactgtt aatattaaca tgggatatcc attataaaat attcatcttt gtagttgata
     1321 attctatatt tcggattttt at
//
LOCUS       DAOBBH010000315         1310 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.317, whole genome shotgun sequence.
ACCESSION   DAOBBH010000315 DAOBBH010000000
VERSION     DAOBBH010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.317"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(546..1190)
                     /locus_tag="P6L01_002638"
     CDS             complement(546..1190)
                     /locus_tag="P6L01_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDP3031651.1"
                     /translation="MSQNTNHSYYHQNQHAQSISKVWLYFMYYWIIFGIGCYLGQFLP
                     LSWRQPLSFGLLIIILATLVFERARRFGLIISHIYAVVIGLLSYATFTTYLQNLGPDI
                     FYKNIALAIFAFIAFGIIGYFFVGDASSIGKYLFVTLITLIIASLIGIFLQNPIFYTI
                     ITVVSLLLFLLYTLYDFNRLKRGDYSPREMGFNLFINLLNIIKDILYLANMFRR"
BASE COUNT          506 a          192 c          141 g          471 t
ORIGIN      
        1 catttaaata ttacaaaaat agatgtagtc agtttaatta aactatccaa ttgaaaacac
       61 actacttttt agtattttca aaataatttt aaatgaccac atctacaacg tattactatt
      121 atcttttgtt agttatatat tcattttcaa tttaaaaata aacacttatt taaatcacat
      181 tctacttttg agatgagctc aaactaacat taaattgttt atattatcta tacttcttcc
      241 tattaatatt tcgattttaa aattaatacg tagctatact ttacttttct aataaaagtg
      301 taattagaaa ggcaagtaca cctgtttaaa gtttatcttc taagatttgt ttactttaga
      361 actatagcag ttaatcattt taatgattaa taatatcttt acaattaagg taataaattt
      421 ttattgcttt atcagtaaaa tctctatcta aatttctaat ttatgacaat cgctccaatt
      481 taaggttgat attcattatt gattattcca aaactttata caaatttctt tattaattta
      541 actgtttatc ttctgaacat attagcaagg taaagtatat ccttaataat attcaacaaa
      601 ttaataaata gattaaatcc catttctctt ggtgaatagt cacctctttt taaacgatta
      661 aaatcataca aagtataaag tagaaataac aacagactaa cgacggtaat aatagtgtaa
      721 aaaataggat tttgaagaaa aataccaatt agactcgcaa taattaatgt tattaatgta
      781 acgaataaat atttgcctat actcgatgca tctccaacga agaaataacc aataatacca
      841 aatgctataa atgcaaaaat tgctaatgcg atatttttat agaaaatatc tggtcctaaa
      901 ttttgtaaat acgtggtaaa cgttgcgtat gacaataagc caatcactac agcgtaaata
      961 tgtgaaataa ttaaaccgaa ccgtctcgct ctttcaaaaa caagtgttgc taaaataata
     1021 atcagtaatc caaatgacaa gggttgtcgc caacttaatg gtaaaaactg acctagatag
     1081 catcctatgc caaatataat ccaataatac ataaaataaa gccacacttt acttattgat
     1141 tgagcatgct ggttttgatg ataatatgaa tgatttgtat tttgcgacaa aataattcct
     1201 ccttctaccg tcgttttatg cgtcaaattt tacagtaaat tttgcttcat caaaagaaat
     1261 aacctttaac aagtatagta attttacatt ttacaatgtt acaaaatata
//
LOCUS       DAOBBH010000316         1307 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.318, whole genome shotgun sequence.
ACCESSION   DAOBBH010000316 DAOBBH010000000
VERSION     DAOBBH010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.318"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            23..1300
                     /locus_tag="P6L01_002639"
     CDS             23..1300
                     /locus_tag="P6L01_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDP3031652.1"
                     /translation="MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDV
                     FLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNL
                     FKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELL
                     PIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTII
                     TFGASALLPLLKLVLVVVFAMVFFVFAILGLVAWMCGINIMNIIRILKSELLLAFSTS
                     SSEAVLPVMMKKMENFGSPKEITSFVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLT
                     LSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRT
                     CVNVIGNALSTIVIAKWENVYDKAKGQEYLKSI"
BASE COUNT          421 a          192 c          242 g          452 t
ORIGIN      
        1 cagatgaagg ggagtttgta ctatggctct attcaagaga aaaattagcc tacctatgca
       61 agttgttatt gcattagtgt taggtgttgt cgtaggactt ttattatatg gacaagaaaa
      121 cgtagcaaat tacattaaac catttggtga cgtattttta aatttaatta aaatgatcgt
      181 tataccagtt gtattttgct cactagcgct ttctatttcg aacgttgggg aatcgaaaac
      241 tgtagggcgt tatggctgga aaacaatttt atactttgaa attattacaa caatcgcaat
      301 aggtttaggg attatcttcg gtaacctatt taaaccaggt gctggattag acccaacaaa
      361 attacctaaa ggtgatattt ctaaatatca atcaactgca catgcagcag aacaatctac
      421 atatggaaat cattttattg ataccattgt acatattatt ccgacaaact tttttgaagc
      481 tttaaataag ggtgaattat tacctattat cttcttcgca gtattctttg gattaggatt
      541 agctgctgta ggtaaaaaag cagaaccagt taaagaattt ttaagcggat cgcttgaagc
      601 tgtgttctgg atgattaata aaattttaaa attagcacca cttggagtgt ttgcattcat
      661 ttgtactaca attattacat ttggtgcatc cgcattatta ccactattaa aattagtatt
      721 agttgttgtc tttgcaatgg tgttctttgt attcgctata ctaggactag ttgcatggat
      781 gtgtggtatt aatatcatga atattattag aatcttgaaa agtgaattgc ttttagcatt
      841 ttctacatca agttcggaag ctgtacttcc tgtaatgatg aagaaaatgg aaaacttcgg
      901 ttctccaaaa gaaattactt cttttgttat accaattggt tatacgttta acttagacgg
      961 atcagcactt tatcaatcta ttgcagcatt attcgttgca cagatgtatg gaatgcactt
     1021 aacattatca gagcaaattg tgttgatgtt aacattaatg attacgtcta aaggtatggc
     1081 ggcagtacca ggaacttcaa tcgttgtttt attaacaaca ttaggtgcca tgggcttacc
     1141 ggcacaaggt ttagcattaa ttattggtgt tgaccgtatc ttagatatgg tacgtacatg
     1201 tgtaaacgtt attggtaacg cattatcaac aatcgttata gctaaatggg aaaacgtata
     1261 tgacaaagca aaaggtcaag aatatttaaa atcaatttaa aaaatac
//
LOCUS       DAOBBH010000317         1280 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.319, whole genome shotgun sequence.
ACCESSION   DAOBBH010000317 DAOBBH010000000
VERSION     DAOBBH010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.319"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..1086)
                     /locus_tag="P6L01_002640"
     CDS             complement(<1..1086)
                     /locus_tag="P6L01_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDP3031653.1"
                     /translation="MFTYFKSAFKNAKPQLLITLIYALIAFAVIAVVYLLANFQLAKY
                     AQTIAIYSQFGQKPPVDAYLKVIAVLLIAAVVSLFVLVQIFIGITNVMKRAMSHEKVK
                     FTDLFIAFKKGNYLKSVLIGLVSIAMIIVLSLLTSLLYKLFSPVSEMIMNSVQSSYAD
                     STHLIGIAITTQSIIIIVVLLIKAIITWLLLIPIFNFMTSFVESTNDKVKTHLANGFK
                     AMKNGQKTFFKFFIGILLLNLIIILFKTPVGYLISFNTQSLSQSVAENIIRVYTVMTI
                     ILFVVIHAIILMGIVQYYLKRGQKITKDKVKTADKDKKVVTEPKNTKVENGKVTTSVE
                     TKTEKAQDSLNDNTTKTMTSDKPEDNQP"
BASE COUNT          462 a          165 c          180 g          473 t
ORIGIN      
        1 tggttgattg tcttctggtt tatctgaagt catcgttttt gttgtattat catttaatga
       61 atcttgtgct ttttctgttt ttgtttcaac agatgttgtc actttaccat tttctacttt
      121 tgtatttttt ggttcagtga caactttttt atccttgtca gctgttttta ctttatcttt
      181 agtaattttt tgaccacgtt ttaagtaata ttgaacgata cccattaaaa tgattgcatg
      241 aataacaacg aataaaatga ttgtcattac tgtataaact cttatgatat tttctgcaac
      301 actttgagag agtgattgcg tattaaatga aatcaagtaa ccaacaggtg ttttaaataa
      361 aataataatt aagtttaata atagtattcc aatgaaaaat ttgaaaaatg ttttttgacc
      421 atttttcatt gctttaaatc cattagctaa gtgtgttttt actttatcgt tagttgattc
      481 aacgaaacta gtcataaagt tgaaaatagg tattaacaat aaccaagtaa taattgcttt
      541 aatcaataaa acaacaatga ttatgatgct ttgagttgta atagcaatac caatcaggtg
      601 tgtactatct gcatatgatg attgtactga attcataatc atttctgata ctggagaaaa
      661 taatttgtat agtaatgatg tcagaagtga aagaacgata atcattgcta tagatacaag
      721 accgatcaga acgctcttta aataattacc ttttttaaat gcaataaata aatcggtaaa
      781 ttttactttt tcatgactca ttgcccgttt cataacattt gtaattccga taaaaatttg
      841 aactaaaaca aacagtgaaa ctacagctgc tataagtaat acagcgataa cttttaaata
      901 tgcatctact ggtggttttt gcccaaattg tgaataaatt gcaatagttt gtgcatactt
      961 agcaagctgg aaattagcaa gtaaatatac aactgcaatt acagcaaatg ctataagtgc
     1021 atatatcaat gttattaata attgtggttt agcattttta aatgctgatt taaagtaagt
     1081 aaacaaagtg gtgcctcctt ttcttcatat aaatacaata acaaaaaaca cgttattttc
     1141 atattaatat ctaataaaaa ttaattaaag tgactaaagt tagagaatta taatagtaaa
     1201 ttgtcaaaaa tatgactaat gtaagaaata atagttgaaa atttgttaat aaacattgca
     1261 tcatcaaaga ccaaacaagt
//
LOCUS       DAOBBH010000318         1268 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.320, whole genome shotgun sequence.
ACCESSION   DAOBBH010000318 DAOBBH010000000
VERSION     DAOBBH010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.320"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(76..807)
                     /locus_tag="P6L01_002641"
     CDS             complement(76..807)
                     /locus_tag="P6L01_002641"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDP3031654.1"
                     /translation="MSKQKKSEIVNRFRKRFDTKMTELGFTYQNIDLYQQAFSHSSFI
                     NDFNMNRLDHNERLEFLGDAVLELTVSRYLFDKHPNLPEGNLTKMRATIVCEPSLVIF
                     ANKIGLNEMILLGKGEEKTGGRTRPSLISDAFEAFIGALYLDQGLDIVWKFAEKVIFP
                     HVEQNELLGVVDFKTQFQEYVHQQNKGDVTYNLIKEEGPAHHRLFTSEVILQGEAIAE
                     GKGKTKKESEQRAAESAYKQLKQIK"
     gene            complement(923..1156)
                     /locus_tag="P6L01_002642"
     CDS             complement(923..1156)
                     /locus_tag="P6L01_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002510960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDP3031655.1"
                     /translation="MENFDKVKDIIVDRLGVDADKVTEDASFKDDLGADSLDIAELVM
                     ELEDEFGTEIPDEEAEKINTVGDAVKFINSLEK"
BASE COUNT          394 a          249 c          158 g          467 t
ORIGIN      
        1 agttgttcca taaaatatta aaaattcttc cttaaatcct aaatcgaaca cctatatgtc
       61 gtatcatata aatttctatt taatttgttt taattgctta taggcacttt cagcagcacg
      121 ttgttctgat tcttttttcg ttttcccttt accttcagct attgcttccc cttgcagaat
      181 aacttctgaa gtgaatagac gatgatgtgc cggtccctct tcttttatta aattataggt
      241 tacatcacct ttattttgct ggtgcacata ttcttggaat tgtgttttaa aatctaccac
      301 gcctaataac tcattttgtt ctacatgtgg gaaaataact ttctcagcaa atttccaaac
      361 tatatctagt ccttgatcca aatacaatgc cccaataaat gcttcgaatg catctgatat
      421 taatgatggt cttgtacgtc cccctgtttt ctcttcacct ttaccaagta aaatcatttc
      481 gttcaatcca attttattcg caaatattac aagtgagggc tcacatacaa tagtggcacg
      541 catttttgtt aaattccctt ctggcaagtt gggatgttta tcaaataaat atcgtgaaac
      601 cgtcaattct aataccgcat cacccaaaaa ctctaaacgc tcattatggt ctaaacgatt
      661 catattaaaa tcattaataa aactcgaatg cgaaaatgct tgttggtata aatcaatatt
      721 ttgataagta aagcctaact ctgtcatttt agtatcaaag cgctttctaa aacgattaac
      781 tatctcactt ttcttttgtt tagacattat tttatctcct ttatgtgttg ctcttgaatc
      841 atctttaatt ttacgttaaa actattgata attaaagaaa aaaactgagt cgacaatact
      901 gacgacccag atgtaagatt tattattttt caagactgtt aataaattta acagcatcac
      961 caacagtgtt gattttttca gcttcttcat caggaatttc agtaccaaac tcgtcttcta
     1021 attccattac taattcagcg atatcaagtg agtcagcgcc taaatcatct ttgaaagatg
     1081 catcttcagt tactttatca gcgtctacac ctaaacggtc aacgatgata tcttttactt
     1141 tatcgaaatt ttccacgtcg attcacctcc tttaaaaatg cctatacata gactattata
     1201 ttttcccatt ttaaattcta aaaaacaagt agaaatttta gaaacactta tcaatttatt
     1261 gattagtg
//
LOCUS       DAOBBH010000319         1229 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.321, whole genome shotgun sequence.
ACCESSION   DAOBBH010000319 DAOBBH010000000
VERSION     DAOBBH010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.321"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..>1229)
                     /locus_tag="P6L01_002643"
     rRNA            complement(<1..>1229)
                     /locus_tag="P6L01_002643"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          282 a          364 c          233 g          350 t
ORIGIN      
        1 gctcagcctt aacgagtacc ggatttgcct aatactcagc cttactgctt agacgtgcaa
       61 tccaatcgca cgcttcgcct atcctactgc gtccccccat cgattaaaac gattataggt
      121 ggtacaggaa tatcaacctg ttatccatcg cctacgcctg tcggcctcag cttaggaccc
      181 gactaaccca gagcggacga gccttcctct ggaaacctta gtcaatcggt ggacgggatt
      241 ctcacccgtc tttcgctact cacaccggca ttctcacttc taagcgctcc acatgtcctt
      301 acgatcatgc ttcaacgccc ttagaacgct ctcctaccat tgtccaaagg acaatccaca
      361 gcttcggtaa tatgtttagc cccggtacat tttcggcgca gtgtcactcg actagtgagc
      421 tattacgcac tctttaaatg atggctgctt ctaagccaac atcctagttg tctgggcaac
      481 gccacatcct tttccactta acatatattt tgggacctta gctggtggtc tgggctgttt
      541 ccctttcgaa cacggacctt atcacccatg ttctgactcc caagttaaat taattggcat
      601 tcggagtttg tctgaattcg gtaacccgag aggggcccct cgtccaaaca gtgctctacc
      661 tccaataatc atcacttgag gctagcccta aagctatttc ggagagaacc agctatctcc
      721 aggttcgatt ggaatttctc cgctaccctc agttcatccg ctcacttttc aacgtaagtc
      781 ggttcggtcc tccattcagt gttacctgaa cttcaacctg accaagggta gatcacctgg
      841 tttcgggtct acgaccaaat actaaacgcc ctattcagac tcgctttcgc tacggctcca
      901 catttactgc ttaaccttgc atcaaatcgt aactcgccgg ttcattctac aaaaggcacg
      961 ccatcaccca ttaacgggct ctgactactt gtaagcacac ggtttcaggt tctatttcac
     1021 tccccttccg gggtgctttt cacctttccc tcacggtact ggttcactat cggtcactag
     1081 agagtattta gccttaggag atggtcctcc cagattccga cggaatttca cgtgctccgt
     1141 cgtactcagg atccactcaa gagagacaac attttcgact acaggattat taccttcttt
     1201 gattcatctt tccagatgat tcgtctaat
//
LOCUS       DAOBBH010000320         1220 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.322, whole genome shotgun sequence.
ACCESSION   DAOBBH010000320 DAOBBH010000000
VERSION     DAOBBH010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.322"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            194..886
                     /locus_tag="P6L01_002644"
     CDS             194..886
                     /locus_tag="P6L01_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDP3031656.1"
                     /translation="MNYSPKQGVRSHGLPHDPFKSSTVPRPIGWISTVSKDGKDNLAP
                     YSQYQNLTWDPPMVMFAANQSVLGDHERKDTVKNAEETGWFVWNMATYDLREAVNLSS
                     KALPPEEDEFEYAGVTKEACIEAPGYRVKESPVHFECEYVQTIRIPTGDPVSTVDIVI
                     GRVAQVHIDDKVILDNGKLDIKSIKPIARLGYYDYTVVNEIFEMKAPAASKEELAGLE
                     GRNFDNQSDEKR"
BASE COUNT          450 a          155 c          224 g          391 t
ORIGIN      
        1 ttaaatttac aaaagatatc ataaatatta attgtataaa tccttatctg aatttttcga
       61 atcaagaacg caaattataa gcaaccacgt catatatagt ttctacttat aaataaattg
      121 tttatttata ttagacgctt atgtaaagag ggcgtaacct taaatgtaat tacttataag
      181 gggtgaagat agtatgaatt attcgccaaa acaaggtgta agaagtcatg gtttaccgca
      241 cgacccattt aaaagtagta cagtacctcg tccaataggt tggatttcta ctgtatcgaa
      301 agatgggaaa gataatttag cgccttatag tcagtatcaa aacttaactt gggatccgcc
      361 tatggttatg ttcgcagcaa atcaatctgt tcttggtgat catgagcgta aagatacagt
      421 aaaaaatgca gaagagacag ggtggtttgt atggaacatg gccacgtatg atttaagaga
      481 agctgttaat ttatcgtcga aggcactacc acctgaagag gatgaatttg aatatgcagg
      541 tgtgacgaaa gaagcatgta tagaagcacc aggttatcga gtcaaagaat cgccagtaca
      601 ttttgaatgt gagtacgttc aaacgattcg tattccgaca ggagacccag tatctaccgt
      661 agatattgtt ataggaagag ttgcccaagt tcatattgac gataaggtta ttcttgataa
      721 tggtaaattg gacattaagt cgattaaacc tattgccaga ttaggatatt acgattacac
      781 agtagtgaat gagatatttg aaatgaaagc acctgcagct tctaaagaag agttagcagg
      841 tttagaagga agaaatttcg ataatcaatc agatgaaaag agataagtaa tttaatcatt
      901 ttagtgaatt caatgcattg ttactaaata ttcaacttag tagcgatgca ttttttcgct
      961 ttataaatga aaatgttaaa gcaatatgtc gtggtattta tatttttaaa gaagaatctt
     1021 ttagaattac acatatatta cactaggttt attgaaaaaa tattggtatt actttaatat
     1081 taaagatgta ttttgcatat aatatttatc gtttagttgt aaattaacta tttataaata
     1141 aaaggtggat gatatagata tattaataca ataattattt taaaaacgaa acaattataa
     1201 tgattaaaca taaagccaat
//
LOCUS       DAOBBH010000321         1201 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.323, whole genome shotgun sequence.
ACCESSION   DAOBBH010000321 DAOBBH010000000
VERSION     DAOBBH010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.323"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(264..1157)
                     /locus_tag="P6L01_002645"
     CDS             complement(264..1157)
                     /locus_tag="P6L01_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDP3031657.1"
                     /translation="MKKTILLTMTTLTLFSMSPNSAQAYTNDSKTLEEAKRAHPNAQF
                     KVNKDTGAYTYTYDKNNTPNNNHQNQSRTNDNHQHANQRDFNNNQYHSSLSGQYTHIN
                     DAIDSHTPPQTSPSNPLTPAIPNVEDNDDELNNAFSKDNKGLITGIDLDELYDELQIA
                     EFNDKAKTADGKPLALGNGKIIDQPLITSKNNLYTAGQCTWYVFDKRAKDGHTISTFW
                     GDAKNWAGQASSNGFKVDRHPTRGSILQTVNGPFGHVAYVEKVNIDGSILISEMNWIG
                     EYIVSSRTISASEVSSYNYIH"
BASE COUNT          340 a          180 c          222 g          459 t
ORIGIN      
        1 ttaaattttg tttaaattaa tagaatttta gttataataa tgtttaataa gttattgaaa
       61 atctaataaa ctacaaaaaa tagtttgatt acataatgat tcttgaaaaa tgtcggttaa
      121 ctttaaggtg acaagttatt gcaattttga aggtaaacat catatttaat aatcgaataa
      181 catacaaaat aaagcgacca attttgcagt gttacgaatt gtaaactgcg aactggtcgc
      241 tttttattga tgtcatgatt aatttaatgg atgtaattat atgatgaaac ttctgaagca
      301 gagatggttc ttgatgaaac gatatattca ccaatccagt tcatttctga aattagaata
      361 cttccatcaa tattaacttt ttcaacgtag gctacatgac caaatggacc atttactgtt
      421 tgtaaaattg atcctcgtgt tgggtgtcta tctactttga agccattgct tgaagcttgg
      481 cctgcccagt ttttagcatc tccccaaaat gtactaatcg tgtgtccatc tttggcacgt
      541 ttatcaaaga cataccatgt acattgtcca gcagtatata agttgttctt acttgtgata
      601 agaggctgat caatgatttt accgttacct aatgctaaag gtttaccgtc agcagtcttt
      661 gctttgtcat taaattcggc gatttgtaat tcgtcataca attcgtctaa atcgatgcct
      721 gtaataagcc ctttgttatc ttttgaaaaa gcgttattta attcatcgtc attgtcttcg
      781 acattcggta ttgctggtgt caaaggattg cttggtgacg tttgaggcgg tgtgtgtgaa
      841 tcaattgcgt cattaatgtg cgtatactga ccacttaatg aagaatggta ctgattgttg
      901 ttaaaatcac gttgatttgc gtgttgatga ttgtcgtttg tacgtgactg gttttgatga
      961 ttgttgtttg gcgtgttgtt tttgtcatat gtataagtat acgcgccggt gtctttattc
     1021 actttgaact gtgcgtttgg gtgtgctctc tttgcttctt ctaatgtttt gctatcattc
     1081 gtatatgctt gagccgagtt aggcgacata ctaaataaag taagagttgt catcgtcagt
     1141 aaaattgttt tcttcataat aaccatttaa tcctttatgt atttaattta attttagtat
     1201 a
//
LOCUS       DAOBBH010000322         1195 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.324, whole genome shotgun sequence.
ACCESSION   DAOBBH010000322 DAOBBH010000000
VERSION     DAOBBH010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.324"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            91..1107
                     /locus_tag="P6L01_002646"
     CDS             91..1107
                     /locus_tag="P6L01_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retron St85 family RNA-directed DNA polymerase"
                     /protein_id="HDP3031658.1"
                     /translation="MEKIENEIVNKTYLAINSLEELRNMIGIKSDYFYKCLYVNDHFY
                     NVIKIPKRKKDEYRELMIPNMALKNIQRWILDNVLYRRQVHKCATGFVPRKSIVNNAI
                     PHVGQKYILKMDIENFFPSITFKQVRKIFSEMGYKFELATALANLCTVNNQLPQGAPT
                     SPYIANIIFYNIDKRIFSYCQKNNLRYTRYADDITISGSNKVSFSKEIIREIVNQYNF
                     RINESKTIMFKPGDRKKVTGIIVNEKISVPKTLIREVRKQIYFVNKFGLEEHLIRNNY
                     SLDYEQQFIMSIYGKISFIKMIDFKKGVSLQKKFNEVLGNIESSNMYRDNIDFDDIEL
                     HWIN"
BASE COUNT          476 a          132 c          196 g          391 t
ORIGIN      
        1 taggggggcc cctaacgact aaataatgta ataattaaaa gaagatggtt tagcaagagt
       61 tgctcattta gataaaaaat ggagaattaa atggagaaaa ttgagaatga aatagttaat
      121 aagacatatc ttgcaattaa ttctttggaa gaattaagaa atatgattgg tattaaatca
      181 gactattttt ataaatgtct ttatgtgaat gaccattttt ataatgttat caaaatacct
      241 aaacgaaaaa aagatgaata tagagaattg atgattccta atatggctct caaaaatatc
      301 caaaggtgga ttttagataa tgtattgtac agaaggcaag ttcataaatg tgcaactggt
      361 tttgttccta ggaaatcaat agtgaacaat gcaatcccac acgttgggca gaaatatata
      421 ttgaaaatgg atatagaaaa cttttttccg agtatcacat ttaagcaggt tagaaaaata
      481 ttttctgaga tgggctataa atttgaactt gcgacagcac tcgcaaattt gtgtacagtg
      541 aataatcagt tgcctcaagg tgcaccaacc agtccatata tagctaatat catcttttac
      601 aatatagata aaagaatctt ttcttattgt caaaaaaata atttgagata tactcgatat
      661 gctgatgata ttactatttc tggatctaat aaagtaagtt ttagtaaaga aattatcagg
      721 gaaatagtaa accaatataa ttttagaatt aatgaaagta agacaattat gtttaaacca
      781 ggagacagaa aaaaagttac tggtattata gttaatgaaa aaatatcggt tcctaaaact
      841 ttaataagag aggtcaggaa gcaaatttat tttgtaaata agtttggatt agaagaacac
      901 ttaattcgaa ataattattc attggattat gagcagcaat ttattatgag tatatatgga
      961 aaaatcagtt ttattaaaat gattgatttc aagaaaggcg ttagtttaca aaagaaattt
     1021 aatgaagtac taggtaatat agaatcgtct aacatgtaca gagataatat tgactttgat
     1081 gatatagaac tacattggat aaattaaacc gcttaataat taatatattt tagttaatat
     1141 ttcttttgaa atttaccacc agccagcaga tttttattat attttaatac cccta
//
LOCUS       DAOBBH010000323         1189 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.325, whole genome shotgun sequence.
ACCESSION   DAOBBH010000323 DAOBBH010000000
VERSION     DAOBBH010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.325"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            186..404
                     /locus_tag="P6L01_002647"
     CDS             186..404
                     /locus_tag="P6L01_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031659.1"
                     /translation="MENQKQGNGLKIATWVFIVLTVVTPLFGIGSIVCSINYKKYDAE
                     KGSKLLQIAIIVTIIAFVLNLLAYLGLR"
     gene            536..1036
                     /locus_tag="P6L01_002648"
     CDS             536..1036
                     /locus_tag="P6L01_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDP3031660.1"
                     /translation="MTFEEKLSQMYNEIANKISSMIPVEWEKVYTMAYIDDEGGEVFY
                     YYTEPGSNELYYYTSVLNKYDISESEFMDSAYELYKQFQNLRNIFKEEGYEPWTSCEF
                     DFTKEGELKVSFDYIDWINTEFDQLGRQNYYMYKKFGVIPEMEYEMEEVKEIEQYIKE
                     QEEAEQ"
     gene            1047..>1189
                     /locus_tag="P6L01_002649"
     CDS             1047..>1189
                     /locus_tag="P6L01_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HDP3031661.1"
                     /translation="MTFEEKISKLYNEIANEISSMIPVEWEKVYTMAYIDDGGGEVFF
                     NYT"
BASE COUNT          477 a          102 c          230 g          380 t
ORIGIN      
        1 ttcagttgtt ggatatttat tttgaatatg attttatcaa gattattata attgtattac
       61 atataggtgt gggtaaattt tatttatgaa atgatgaaaa agtatatact attaatatct
      121 tttttactat aattataaat agttaaataa attgaaagac aaaataatga gtaggagtta
      181 atgagatgga aaaccaaaaa caaggcaatg gcttaaaaat tgcaacatgg gtatttattg
      241 tattaacagt agttacaccg ctatttggta ttggaagtat tgtttgtagt attaattata
      301 aaaaatacga tgctgaaaaa ggttcgaagt tattgcaaat tgcaattatc gtaacaataa
      361 ttgcttttgt tttaaattta ttagcatatt taggtttaag ataagtaaaa cgaatttgaa
      421 gaagcattaa gcgacatttg ggtgttgttt aatgcttttt atttaacgga aataagtgta
      481 gagaataaat taatagttta atcaagagat atttcgaaca cataggggcg ataacatgac
      541 tttcgaagaa aaattaagtc aaatgtacaa cgagattgcg aataagatta gtagcatgat
      601 accagtagag tgggaaaaag tatatacaat ggcttatata gatgatgaag gtggagaagt
      661 gttctactat tacacagaac ctggaagcaa tgaattatac tactatacta gtgtattaaa
      721 taaatatgat atatcggaat cagaatttat ggactcagcg tatgagttgt ataaacaatt
      781 tcaaaattta agaaatatat ttaaagaaga aggatatgaa ccatggacat catgcgaatt
      841 tgattttaca aaagaaggtg aattaaaagt ttcatttgat tatatagatt ggatcaatac
      901 agagtttgat caattgggcc gtcaaaatta ttatatgtac aaaaaatttg gggttatacc
      961 agaaatggaa tatgaaatgg aagaagttaa agaaatcgaa caatatatta aagagcaaga
     1021 agaagctgaa caatagaggt gataacatga ctttcgaaga gaaaataagc aaattatata
     1081 atgagattgc gaatgagatt agcagtatga taccggtaga gtgggaaaaa gtatatacaa
     1141 tggcttatat agatgatggc ggaggtgaag tattctttaa ttatactaa
//
LOCUS       DAOBBH010000324         1162 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.326, whole genome shotgun sequence.
ACCESSION   DAOBBH010000324 DAOBBH010000000
VERSION     DAOBBH010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.326"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            5..433
                     /locus_tag="P6L01_002650"
     CDS             5..433
                     /locus_tag="P6L01_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031662.1"
                     /translation="MTRSALKPFKNKRVMVTGRIQRVLFKNYLDRHSTFKPNVRILLK
                     DVFVSGVSIDHLWLYETNKYYALAMELIHQRVKFSANVVPYYKINRNNNLFVQDYGIK
                     RKGRLITEEAYNQNNQYQDKIYEKLPDIDFRLEDFYSKEN"
     gene            complement(642..1148)
                     /locus_tag="P6L01_002651"
     CDS             complement(642..1148)
                     /locus_tag="P6L01_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDP3031663.1"
                     /translation="MAHIIRRVSIKDVENFISMLAKIYDESPYMFYTPGEYDPSVTSA
                     SKQLEEYITSPHKVIFVAESDEQLVGFAFVNTTPFQRIKHVAKIDLGVKKLYQHRGIG
                     QALLDAIMAWCLNNQIHRIEANVPLNNQPALELFKSADFQIEGVLKDKLFIDGKYYDD
                     YMMAKILN"
BASE COUNT          417 a          157 c          202 g          386 t
ORIGIN      
        1 gggaatgaca aggtcagcat taaaaccatt taaaaataaa cgcgttatgg ttactggacg
       61 tatacaacgt gttttgttta aaaattattt agatagacat agcacattta agccgaatgt
      121 aaggatatta ttaaaagatg tatttgtttc aggtgtatca atagatcatt tatggttata
      181 tgagacaaat aaatactatg cattggcaat ggaacttatt catcaacgag taaaatttag
      241 tgcgaatgtt gtaccatatt acaaaataaa tagaaataat aatttattcg tacaagatta
      301 tggaattaag cgtaaaggta ggttaattac tgaagaagct tacaatcaaa acaatcagta
      361 tcaggataag atatatgaaa aattaccgga tatagatttt agactcgaag atttttatag
      421 taaggaaaac taaacaacat atataaagca ctactaatcg tagttgatag ttgatcaatt
      481 acacattagt ggtgctttat tttgaattta aatttgataa atgaataaga gtatacgcat
      541 tcatttaaga ttagatgaca ttgataagta tgagtaatgt attaggtaac cattaaattc
      601 gttgttatgc gattctgata caagattatg ataaaatagc tttaattaag aattttagcc
      661 atcatatagt catcgtaata tttaccatcg ataaataact tatcttttaa aacgccttcg
      721 atttgaaaat cggcactttt aaaaagctcg agggcaggtt ggttattgag tggtacattt
      781 gcttcaattc ggtgtatttg attgtttaaa caccaagcca taatggcatc aagaagtgct
      841 tggccaattc cacgatgttg atataatttc tttacaccta aatcaatttt agcaacatgt
      901 ttaatgcgtt gaaatggtgt cgtattaaca aaggcaaagc caacgagttg ttcatcactt
      961 tcagcaacaa agatgacttt atgtggagaa gtgatatatt cttctaattg tttactagcc
     1021 gacgtgacgc taggatcata ttctcctggt gtgtagaaca tatacggaga ttcgtcgtat
     1081 attttcgcta acattgaaat gaaattttct acatctttga tactaactct acgtataata
     1141 tgggccataa aaagcctcca gt
//
LOCUS       DAOBBH010000325         1160 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.327, whole genome shotgun sequence.
ACCESSION   DAOBBH010000325 DAOBBH010000000
VERSION     DAOBBH010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.327"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            431..532
                     /locus_tag="P6L01_002652"
     CDS             431..532
                     /locus_tag="P6L01_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031664.1"
                     /translation="MKFVANKLTLTIFRKFCINNEKEIWIKYEEVFL"
     gene            542..814
                     /locus_tag="P6L01_002653"
     CDS             542..814
                     /locus_tag="P6L01_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HDP3031665.1"
                     /translation="MFGTILYLTLALGLSTAAYASTEYAEGGTWSHGVGSKYVWSYYY
                     HGHKGHGATAIGKYRSFSGYTRAGVKAKASATKHNCWVNRAYYNIY"
     gene            858..>1160
                     /locus_tag="P6L01_002654"
     CDS             858..>1160
                     /locus_tag="P6L01_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HDP3031666.1"
                     /translation="MKWFKLILDVTTFILIAILLFVYTYKENEEILPDTKYPIAVTDW
                     NKKYSKNEIYKRINQFAKNENVAIYKSTSNYTNKNVDKDIYVFNKSKATTITPFNAK"
BASE COUNT          454 a          151 c          176 g          379 t
ORIGIN      
        1 acatttttat agtaacatat cttttattta attgtctatt tttattagat gtgtaacaaa
       61 attttgaagt attttccgta tattgcctgc taaacatgtc agatttgaaa ggaaaatcat
      121 actgtggtag aaatcaatta tgattatatg gtagtactta taaataagtg atttctattg
      181 aatatgtaat gaagttcata tattaatata actttcattg atatagaagt cgcaatgatt
      241 aatttaaaca ataaaaaaca accatcattt caaaactttg acaacgaatg atggttaaag
      301 aaaatctcat ttagtcaccg tctttttaac gggttcaata ggggacatgt tcctgcatgt
      361 cgtctattta tgcactgcgc aatttaacaa taatacaatt atactttgat gtccatttaa
      421 cataaagtgg ttgaaatttg tagcaaataa attaaccttg actattttca gaaaattctg
      481 tataaataac gaaaaagaga tatggattaa atatgaagag gtgtttttat agaagaataa
      541 aatttttggc actatattat atttaacttt agcacttgga ttatcaacag cagcttatgc
      601 atctacagaa tacgcagaag gaggcacttg gagtcacggt gtcggcagta agtatgtttg
      661 gtcttattat tatcatggtc ataaaggaca tggtgcaaca gctattggaa aatatagatc
      721 atttagtggt tatacaagag ctggtgtaaa agcaaaagca tcagctacta aacataattg
      781 ctgggtcaat agagcgtatt ataacattta ttaaatacta aaaaatcata gaaaataaac
      841 aacgcaagag gtacaacatg aaatggttca aattaatact tgatgttaca accttcattc
      901 tgattgctat attgttattt gtttatacat ataaagaaaa cgaagaaata ttgcctgata
      961 ctaaataccc tatagcggta actgactgga ataaaaaata tagtaaaaat gagatttata
     1021 aacgtataaa tcaattcgct aaaaatgaga acgtagcaat ctataaatca acttcaaatt
     1081 atacaaacaa aaacgtagat aaagatatat atgtatttaa taaatcaaaa gcaacaacta
     1141 tcactccttt taacgctaaa
//
LOCUS       DAOBBH010000326         1153 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.328, whole genome shotgun sequence.
ACCESSION   DAOBBH010000326 DAOBBH010000000
VERSION     DAOBBH010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.328"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            158..955
                     /locus_tag="P6L01_002655"
     CDS             158..955
                     /locus_tag="P6L01_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDP3031667.1"
                     /translation="MKKLAFAITATSGAAAFLTHHDAQASTQHTVQSGESLWSIAQKY
                     NTSVESIKQNNQLDNNLVFPGQVISVGGSDAQNTSNTSPQAGSASSHTVQAGESLNII
                     ASRYGVSVDQLMAANNLRGYLIMPNQTLQIPNGGSGGTTPTATTGSNGNASSFNHQNL
                     YTAGQCTWYVFDRRAQAGSPISTYWSDAKYWAGNAANDGYQVNNTPSVGSIMQSTPGP
                     YGHVAYVERVNGDGSILISEMNYTYGPYNMNYRTIPASEVSSYAFIH"
BASE COUNT          407 a          200 c          180 g          366 t
ORIGIN      
        1 aaatgttaat aatattacaa gataataaca aaccacacaa agctacttat ttttgataat
       61 atggaaatcg taatataaaa cgaaaactta atttactata taaattgtct taataatttt
      121 taaaagtagt aaaacttaat tttaaggagg agtccctttg aaaaaattag catttgcaat
      181 aacagcaaca tctggtgcag ctgcattttt aacgcatcat gatgcacaag cttctacaca
      241 acatacagta caatctggtg aatcattatg gagtattgct caaaaataca acacttcagt
      301 agagagtatt aaacaaaata accaattaga taacaacttg gtattccctg gtcaagttat
      361 ctcagtaggt ggaagtgatg cacaaaatac gtcaaacact tctccacaag ctggttcagc
      421 atcatctcat actgtacaag ctggtgaatc attaaatatc attgctagca gatatggtgt
      481 ttcagttgat caattaatgg cagccaataa cttacgtggt tatttaatta tgcctaacca
      541 aacattacaa attcctaatg gtggatcagg tggtacaaca ccaacagcta caacaggtag
      601 caatggcaat gcatcatctt ttaatcacca aaatttatac actgctggtc aatgtacatg
      661 gtacgtattt gaccgtcgtg ctcaagctgg tagtccaatt agcacatatt ggtcagacgc
      721 taagtattgg gctggtaacg cagctaatga tggttaccaa gtaaacaaca caccatcagt
      781 tggttcaatt atgcaaagca cacctggtcc atatggtcat gttgcttatg ttgaacgtgt
      841 caatggtgat ggtagtatct tgatttctga aatgaattac acatatggtc catacaatat
      901 gaactaccgt acaattccag cttcagaagt ttctagctat gcattcatcc attaattaaa
      961 taaattgtac tgatatatac tagcaattca catcatgtga gattgctagt tttttatttt
     1021 tgaaaaaaat tttcattttg gtacaaaaaa ttatctcacc cttccctatc atacttattt
     1081 atattttgta tgaatggtag ttaggtaaaa aataataacc tacctatttg attaaattaa
     1141 atgcataata tat
//
LOCUS       DAOBBH010000327         1151 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.329, whole genome shotgun sequence.
ACCESSION   DAOBBH010000327 DAOBBH010000000
VERSION     DAOBBH010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.329"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(305..1000)
                     /locus_tag="P6L01_002656"
     CDS             complement(305..1000)
                     /locus_tag="P6L01_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDP3031668.1"
                     /translation="MQSKILIIEDDHAITHLLDVALTLDYYNVTTADNATQAHFKIQI
                     DKPDVILLDLGLPDKDGLCLISEIRQHTDIPIIVISARQEEQTIIQALDNGANDYMTK
                     PFNVDELRARIRVIERIAKSHQETNIVFTNGLLSIDFGSKSVVINNQEVHLTPNEFSL
                     LELLSNHKGKVLTYEMILKRIYGYVNKTEMPSLRVHMTSLRQKLSQCHEDAKDIIKTH
                     PRIGYQMLQWKEK"
BASE COUNT          356 a          172 c          184 g          439 t
ORIGIN      
        1 ttttcaataa ttgattctct gtagatatca aagtactaac gttttaaagg ttaaatattt
       61 aattggattg agatctgtat gcggttatat cattctgtgt aaatataaat atgaatctag
      121 aatataacaa ctacttaaat taagaataat cgaacataaa taaactttat gaaatttcag
      181 tatcatgttc ttataaaaaa caatagggct ttttgctgac gctagtgcgc gataaataat
      241 aagttgaata taaaaaagat cactgccaat cattcgttta atggcagcga tcttttttat
      301 ttaattattt ctctttccac tgcaacattt gataaccaat gcgtggatgt gttttaataa
      361 tatcttttgc gtcctcatga cattgtgaaa gtttttgtct taatgatgtc atatgcactc
      421 ttaaactagg catttcagtc ttattaacat agccatatat tcgttttaat atcatctcat
      481 aagtgagtac ttttccttta tgatttgaca atagttctaa caagctaaat tcatttggcg
      541 tcaaatgtac ctcctgatta ttaataacaa cagatttgga gccaaagtcg atgcttagca
      601 aaccgttagt aaatacaatg ttagtttctt gatgtgactt agcgattctc tcgatgactc
      661 gtattcgtgc ccgaagctca tcaacattaa aaggtttagt catatagtca ttcgcaccgt
      721 tatctaaagc ttgaataatt gtttgttctt cttgtcttgc acttattaca atgataggaa
      781 tgtcagtatg ttgcctgatt tctgaaatca aacataatcc atctttatct ggtaaaccta
      841 aatctaataa aatgacatct ggtttatcaa tttgaatttt aaagtgtgct tgtgtggcat
      901 tgtcggctgt agttacattg taataatcta aagttaatgc aacatcaagt aaatgtgtga
      961 ttgcgtgatc atcttcaatt atcaatattt tagattgcat tatacgtctc cttcgttaaa
     1021 gtctgtatat atattgaaat agaatatact gccgtgtggt tggttcggtt tatattgtaa
     1081 gtttgattga tgtttgtgta ggatagtctg tactaaatat aagcctagtc ccatgctttc
     1141 tttttggtta t
//
LOCUS       DAOBBH010000328         1138 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.330, whole genome shotgun sequence.
ACCESSION   DAOBBH010000328 DAOBBH010000000
VERSION     DAOBBH010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.330"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            1..246
                     /locus_tag="P6L01_002657"
                     /pseudo
     CDS             1..246
                     /locus_tag="P6L01_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463904.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            331..683
                     /locus_tag="P6L01_002658"
                     /pseudo
     CDS             331..683
                     /locus_tag="P6L01_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891995.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            690..884
                     /locus_tag="P6L01_002659"
     CDS             690..884
                     /locus_tag="P6L01_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain protein"
                     /protein_id="HDP3031669.1"
                     /translation="MNLQSYIGKLVKLTLTNNKILIGKVIDFDDKVDNFDGYNSIEID
                     TGRITYDISENKIKTIVLQK"
BASE COUNT          479 a          138 c          169 g          352 t
ORIGIN      
        1 tgctattttt atatgctttt ttcattattc gttgaaaaag aagagtcaca aaagttaaca
       61 tatttaaaag aacttggtga agatgacgaa tataaatatg ttgccaaaat agatagtaaa
      121 acatctaaat tatgtcattc actcaacgga aaaatattta tagttaaaga tatgatacca
      181 ggtgtgaatg cgccacctat gcatccttgg tgtagaagta ccacagtgcc acatgttggc
      241 aattggtgag acaagttcat taaagagcgt gaaggtaagt atcaagtaga agtaaaagaa
      301 gcaaaattac aagaaaaagc taaaaaagaa atgcttgata tgataagtaa tggtaaaata
      361 aagatgcaat tgaataatga aaaacaacag cgtcatattt taaacacaaa tgaatataat
      421 aacaagaaaa ataattcatc tttcttaccc agttatatta caatagattc taatgaaatt
      481 gaaaaggtga ctaaaaaaga atttattgat tttccagttt tgtttgatga tgaagggaat
      541 ttcagaaaaa aacaagttat agattacaaa aagataatag gtaaatctta tgtgaatggt
      601 aaatatatgt tgaaacgaaa ttaggtaaag ttcattattc taaaactggt tttcatgtag
      661 ttccgtatat tgaaaaggag tgattaaata tgaatttaca gtcatacata ggcaaattag
      721 tcaaattaac gcttactaat aataaaatat taattggaaa agtgatagac tttgatgata
      781 aagttgataa ttttgatggt tataattcga tagaaattga tacaggtaga attacatatg
      841 atatatcaga aaataaaatt aaaactattg ttttacaaaa atagcatcct ttctacacag
      901 ataaagagaa agcggtgcta ttttttgcac ttttataaca actaaggata caagacatat
      961 accttcaaca atatcctcat ttaaacctat cgatttccta agctcaattt taaacacaaa
     1021 gtttatttga aatgatgcac acctataaaa atcttaatca tcgaaaagtc acaaacatct
     1081 ccatattaaa aacttaaata gtgcatttca tttaaactaa acatagatat tcttacaa
//
LOCUS       DAOBBH010000329         1125 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.331, whole genome shotgun sequence.
ACCESSION   DAOBBH010000329 DAOBBH010000000
VERSION     DAOBBH010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.331"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            233..667
                     /locus_tag="P6L01_002660"
     CDS             233..667
                     /locus_tag="P6L01_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDP3031670.1"
                     /translation="MKLKSFITVTLALGMIATTGATVTSNEVSAAEKDKLPATQKAKE
                     MQNVPYTIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLYDERGVTPEKIRNAKS
                     AVYTITWKDGSKKEVDLKKDSYTANLFDSNSIKQIDINVKTK"
     gene            complement(924..1109)
                     /locus_tag="P6L01_002661"
     CDS             complement(924..1109)
                     /locus_tag="P6L01_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDP3031671.1"
                     /translation="MKHLVTFFWALLLMQMVNFVLNSLVGGDSLNVVNPIIMAVLFTI
                     FTAIFAAVIKPPKDSSQ"
BASE COUNT          436 a          153 c          173 g          363 t
ORIGIN      
        1 ggcgttcgct ataatgcaaa gaagtaatta aatttaagaa atgtaaactt agttattgta
       61 atgtgaattt atttgaaaaa atagaaagta ttaacaatta tagcttttac attaattaaa
      121 atttattttt aaaaacaagt aaacaattta catacttata atttttgaaa cttttagatt
      181 tgtgttatat tgattttgta agatacttta actcacaaag gagagagatt atatgaaatt
      241 aaaatcattt ataactgtaa ctttggcact gggcatgatc gcaacgactg gcgctactgt
      301 gacaagtaat gaggtatctg cagcagaaaa ggacaaacta ccggcaactc aaaaagctaa
      361 agaaatgcaa aatgttccat atacaattgc agtagatggc attatggctt tcaatcaatc
      421 ttacttaaat ttaccaaaag atagccaatt atcatattta gatttaggaa ataaagttaa
      481 agctttatta tatgatgaac gtggtgtaac acctgagaag attcgaaatg caaaatctgc
      541 cgtttacacg attacttgga aagatggtag taaaaaagaa gtggatctta agaaagatag
      601 ctacacagca aacttgtttg attcaaattc aattaaacaa attgatatta atgtaaaaac
      661 taaataaaca cttaaaatta tcaaattcaa catgaagcat ttaattttac agtgatgatt
      721 ataatataat tgccttgata caaagattac tcgtaaatga catctttgta ttaaggcttt
      781 ttctaaattt aaaagtgatg ggttagaggt tattgagctt taaaatattc aaaatacaaa
      841 acattaatgg ccaaaaataa aagccgcctt tatctgggca gcttcaataa taagaaagac
      901 atatttcatt ttatactaaa tagttattgt gatgaatctt tcggcggttt aattactgca
      961 gcaaaaattg ctgtgaaaat cgtgaacaat actgccatga taattggatt cactacattt
     1021 aagctgtctc cacctactag gctattaagt acaaagttaa ccatttgcat taataataat
     1081 gcccaaaaga atgttacgag gtgtttcatg tcattctacc tccac
//
LOCUS       DAOBBH010000330         1115 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.332, whole genome shotgun sequence.
ACCESSION   DAOBBH010000330 DAOBBH010000000
VERSION     DAOBBH010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.332"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(94..849)
                     /locus_tag="P6L01_002662"
     CDS             complement(94..849)
                     /locus_tag="P6L01_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDP3031672.1"
                     /translation="MKTYSEARARLRWYQGRYIDFDGWYGYQCADLAVDYIYWLLEIR
                     MWGNAKDAINNDFKNMATVYENTPSFVPQIGDVAVFTKGIYKQYGHIGLVFNGGNTNQ
                     FLILEQNYDGNANTPAKLRWDNYYGCTHFIRPKYKSEGLMNKITNKVKPPAQKAVGKS
                     ASKITVGSKAPYNLKWSKGAYFNAKIDGLGATSATRYGDNRTNYRFDVGQAVYAPGTL
                     IYVFEIIDGWCRIYWNNHNEWIWHERLIVKEVF"
     gene            complement(861..1115)
                     /locus_tag="P6L01_002663"
     CDS             complement(861..1115)
                     /locus_tag="P6L01_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDP3031673.1"
                     /translation="MINWKIRMKQKSFWVAILSAIFLFAQNIAKAIGYDIQVYTEQLT
                     DGLNAILGFLVLTGVIQDPTTKGIGDSHQALEYEEPRRKY"
BASE COUNT          359 a          224 c          139 g          393 t
ORIGIN      
        1 attgagaaaa tattaaaaat caataaaata ttaaaagaaa ctaaacttac attaaaaaat
       61 aattaacaaa tatttaacat tttaacctaa gaattaaaac acttctttca caatcaatct
      121 ctcatgccat atccactcat tatgattgtt ccaataaatg cgacaccaac catctataat
      181 ttcaaacaca tatattaatg ttccaggcgc gtatacagcc tgtccaacat cgaatctata
      241 gttagtacga ttatcaccgt atctagtggc tgaagtagca cctaagccgt cgattttcgc
      301 attaaaataa gcaccttttg accatttaag gttataaggc gctttacttc caactgttat
      361 tttacttgca gatttaccga ctgctttttg agcaggtggt ttaactttat ttgtgatctt
      421 attcattaag ccctcacttt tatacttagg tctaataaag tgagtacagc cgtaataatt
      481 atcccaacgt aactttgcag gcgtatttgc gttaccgtca tagttctgtt ccaaaattaa
      541 aaattgattt gtattaccac cattaaacac taaaccaata tgaccgtatt gtttatatat
      601 tcctttggta aatacagcca catcacctat ttgtggaaca aacgatggtg tgttttcata
      661 tactgttgcc atgtttttaa aatcgttatt gattgcatct tttgcatttc cccacattct
      721 aatttctaac aaccaataaa tgtaatcaac tgctaaatct gcacattggt aaccatacca
      781 accgtcaaaa tcaatatatc taccttgata ccaacgtaac cttgctcttg cttcactgta
      841 tgttttcatt attttacctc ctagtatttt cttcttggtt cttcatattc taaagcttgg
      901 tggctatcac ctataccttt agtagtcggg tcttgaatca caccagttaa tactaaaaat
      961 cctaatatag cgtttaaacc gtctgttaat tgctctgtat aaacttgaat atcataccca
     1021 atagctttgg cgatgttttg agcaaataaa aagatagctg acaatatcgc tacccaaaat
     1081 gatttttgtt tcattctaat tttccaatta atcat
//
LOCUS       DAOBBH010000331         1099 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.333, whole genome shotgun sequence.
ACCESSION   DAOBBH010000331 DAOBBH010000000
VERSION     DAOBBH010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.333"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            9..1034
                     /gene="gap"
                     /locus_tag="P6L01_002664"
     CDS             9..1034
                     /gene="gap"
                     /locus_tag="P6L01_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDP3031674.1"
                     /translation="MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHL
                     INYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNH
                     GDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDNQLDIEAFDIFSNASCTTNCIGP
                     VAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALK
                     EVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQ
                     PLVSVDFNTNPNSAIIDAKSTMVMSGNKVKVIAWYDNEWGYSNRVVDVAEQIGALLTS
                     KETVSAS"
BASE COUNT          406 a          147 c          206 g          340 t
ORIGIN      
        1 tgcttttaat gtcaacgaat attgcaatta atggtatggg tagaattgga agaatggtat
       61 tacgtattgc attacaaaat aaaaatttaa atgtagtagc gataaatgct agttatccac
      121 ccgaaacaat tgcacattta atcaattacg atacaacaca tggaaaatat aatctaaaag
      181 ttgaaccgat tgaaaatgga ttgcaagttg gtgatcataa aattaaattg gttgctgatc
      241 gcaatcctga aaacttgcca tggaaagaat tagatatcga tattgctata gatgcaactg
      301 gtaaatttaa tcatggtgat aaagccatcg cacatattaa agcaggtgcc aaaaaagtat
      361 tgttaactgg tccttcaaaa ggtggacatg ttcaaatggt agttaaaggc gtaaatgata
      421 accaattaga tatagaagca tttgacattt ttagtaatgc ttcatgtact actaattgca
      481 ttggtccagt tgcaaaagtt ttaaataatc agtttggaat agttaatggt ttaatgacta
      541 ctgttcacgc tattacaaat gaccaaaaaa atattgataa tccacataaa gatttaagac
      601 gtgcacgttc atgtaatgaa agcattattc ctacttctac tggtgcggcg aaagctttaa
      661 aagaagtatt accagaatta gaaggtaaat tacacggcat ggcattacgt gtaccaacaa
      721 agaatgtatc gctcgttgat ttagttgttg atttagaaaa agaagtaact gcagaagaag
      781 taaaccaagc ttttgaaaat gcaggtttag aaggtatcat agaagtcgaa catcaaccac
      841 tagtgtctgt tgattttaat actaatccaa attcagctat tattgatgca aaatcaacaa
      901 tggtcatgtc agggaataaa gtaaaagtta ttgcttggta tgataatgaa tggggttatt
      961 caaatagagt tgtagatgtt gctgagcaaa ttggagcact tttgacatca aaagaaactg
     1021 taagtgcaag ttaatacaat taatttatag cctaactctg acttcggagt taggtttttt
     1081 attttttaat acaataata
//
LOCUS       DAOBBH010000332         1095 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.334, whole genome shotgun sequence.
ACCESSION   DAOBBH010000332 DAOBBH010000000
VERSION     DAOBBH010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.334"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
BASE COUNT          424 a          180 c          135 g          356 t
ORIGIN      
        1 cacaataaat tttctcggca taaatgcgtg tctatgcgta gacatggaga cacttcagaa
       61 ttatggagtg attacatgag tttaactatt tttctttatt cagttttagg aagtttatta
      121 gcaaattata tctacgaaaa atcttcaaaa taaataactt tttcggttct actttgtaca
      181 ctttatctac ctggtagacc taattgttat tgctttacag gtctaaaatt cgtcgttata
      241 atctagttaa atcccgtaag ggcgcactta tacgtcggat aaatcgacat gtgcaaaaac
      301 gtaatcgacc ttcgtcgatt tttttatttc aaaattaaag aaacggcttg agatattccg
      361 agcagttata aaaattaatt tgcgttgaat attgctacta cattcttgaa aaagtgatgt
      421 agatttatag cccataactt ttttgaatta catcgacgat acggcgtcga aagaaaaata
      481 aatagaccgt atgaaagaaa aagctacctc actttatctg gggtagcttt tattttttat
      541 gcccttattt tgaattttaa gagggcattt taaagattta ggggtgattc atatagtttt
      601 atatctaaaa cttatatatg cttttaaaac gcaaatatga gccaaataaa tatatgctat
      661 tttcaaaaac aaaaatttga gcaaaaacaa taactatttt tagacactgc ccatttacat
      721 gcaaattaaa aattagcata aaattcttta aaacttcccc acgtttttct aaattcctgt
      781 agggcgtttt tatcaaaaat caagacaaga agaaactcgt tcacactcgt ttcaaaaaaa
      841 caagaaatct aatctataca cctaccgttt tccaaccccc tcaaagcata caaattaaaa
      901 acaaaattat tcataatctt actttctttt aacaaaaaca taaaaaccat aaatagacaa
      961 attaactatc aaaggcaata aacagtataa aaaaaccacg aataaatctt catcaacatc
     1021 taaaataaaa aagaataaaa caattaacaa aatactaatt aacaaaccta aaagtggcaa
     1081 actttttata tattt
//
LOCUS       DAOBBH010000333         1090 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.335, whole genome shotgun sequence.
ACCESSION   DAOBBH010000333 DAOBBH010000000
VERSION     DAOBBH010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.335"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(293..>1090)
                     /locus_tag="P6L01_002665"
     CDS             complement(293..>1090)
                     /locus_tag="P6L01_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDP3031675.1"
                     /translation="IFLAQSGGSREGLNFKEIANLKIFTPTIFEEQQKIGQFFSKLDQ
                     QIELEEQKLELLQQQKKCYIQKIFSQELRFKDEEGNYYKGWNKKQLKDVLEFSNKRTI
                     NENEYPVLTSSRQGLILQSDYYKDRKTFAESNIGYFILPKNHITYRSRSDDGIFKFNL
                     NLMIDVGIISKYYPVFKGIDANQYYLTLHLNYQLKKEYIKYATGTSQLVLSQKDLQNI
                     KTKLPSYEEQQKIGDFFSEIDRLVEKQSSKVGRLKVRKKELLQKMFV"
BASE COUNT          328 a          159 c          136 g          467 t
ORIGIN      
        1 atgatgttag attatattct cgtataattt cgtttctagg cttaccattt tcataaagtt
       61 taattatttt aatttaaatt atttactaaa agctcttcag tctcttgtca caataaaatc
      121 gcctaacctc ttagtttaac aatatctatt cccatagaat ttgtccaact aagtgtagac
      181 gattcactaa actccaaaaa aataaatccc aaattataat agtttcaata ttaatatatt
      241 acaatttatt catagaattt ttatcatttt aattaatagg ctgttatctg gattaaacaa
      301 acattttttg taatagttct tttttacgta cttttaatcg tccgactttt gaagattgtt
      361 tttcaaccaa tctatctatt tcactgaaaa aatcaccgat tttttgttgt tcttcataag
      421 atggcaattt agtctttatg ttttgcaagt ctttttgtga gagtaccaat tgtgatgtac
      481 cagttgcata tttaatatat tcttttttca gttgatagtt taagtgtaat gttaaataat
      541 attgatttgc atctatccct ttaaagacag ggtaatattt actaataata cctacatcaa
      601 tcattagatt taaattaaac ttaaaaattc catcgtcgct tcttgaacgg tatgttatat
      661 gatttttagg gagtatgaaa tacccaatat tactctctgc aaaagttttc ctatctttat
      721 agtagtctga ctgaagtatt aaaccttgtc ttgacgatgt taaaacagga tattcatttt
      781 cattaatagt tcttttatta ctaaattcta atacatcttt taattgcttt ttgttccatc
      841 ctttatagta attaccttct tcatctttga atcgtaattc ttgtgagaag attttctgta
      901 tatagcattt tttctgttgt tgaagtaatt caagtttttg ttcttctaat tcaatttgtt
      961 ggtcaagttt gctgaagaat tgtcctattt tttgttgttc ttcaaatata gttggggtga
     1021 agatttttaa attagcaatt tctttgaagt ttagtccttc tcgactacct ccactttgtg
     1081 caaggaaaat
//
LOCUS       DAOBBH010000334         1089 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.336, whole genome shotgun sequence.
ACCESSION   DAOBBH010000334 DAOBBH010000000
VERSION     DAOBBH010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.336"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            9..152
                     /locus_tag="P6L01_002666"
     CDS             9..152
                     /locus_tag="P6L01_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031676.1"
                     /translation="MLQKFRISKEKNKLKLKLLKHASYCLETSNNPELLRAVAELLIK
                     INR"
     gene            complement(173..730)
                     /locus_tag="P6L01_002667"
     CDS             complement(173..730)
                     /locus_tag="P6L01_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031677.1"
                     /translation="MECLLELLKMSIPLIAVIFSWWLATKTANEKFEEQIKKETYDNF
                     YSEILKACYELPSTDLLDFMSFYSYHRKDKISEIIIKNFSYVPPDIVNYWKKYNLALK
                     FFEHDYEKDVQAREVLSRFLNYYSHLIIIKSLEESEKLSEELKLPQLSTQLLSPLKRI
                     DFYKSQLPSQKLILKYHLQELVPNS"
     gene            801..1022
                     /locus_tag="P6L01_002668"
     CDS             801..1022
                     /locus_tag="P6L01_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031678.1"
                     /translation="MMKNSLQAKELAVILSVSKSKAGQIIRELNKELEDEGYIAIRGR
                     IPVQLARKKFPYHDLSDQRIIEELKKVNE"
     gene            1015..>1089
                     /locus_tag="P6L01_002669"
     CDS             1015..>1089
                     /locus_tag="P6L01_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 family protein"
                     /protein_id="HDP3031679.1"
                     /translation="MSKTYKSYLLAVLCFTVLAIVLMPL"
BASE COUNT          401 a          157 c          187 g          344 t
ORIGIN      
        1 cacaacaaat gttacaaaaa tttagaatct cgaaagaaaa aaataaatta aaactcaaat
       61 tactaaagca tgctagttac tgtttggaaa caagtaacaa ccctgaactg ttgcgagcag
      121 ttgcagagtt gcttataaag ataaatcgat aaatagcgaa ggagttgatt ttttatgaat
      181 tcggaactaa ttcttgcagg tggtacttta ggattaattt ttgtgacggt aattgagact
      241 tataaaagtc gatcctcttt aaaggagaaa gtaattgtgt cgatagctgc ggtaatttca
      301 gttcttctga taatttctct gattcttcta acgatttaat aattatcagg tgagaataat
      361 aatttaaaaa tcgacttaaa acttctctgg cttgtacatc tttttcataa tcatgttcga
      421 aaaatttaag tgctagattg tatttcttcc aatagttaac tatatcggga ggtacataac
      481 taaagttttt aatgattatt tcagaaatct tatctttgcg gtgataagag taaaaactca
      541 tgaagtctaa taaatcggta gaaggcaact cataacaagc ttttagaatc tcggagtaaa
      601 aattatcata ggtctctttt ttgatttgtt cttcgaattt ttcattagcc gtttttgtag
      661 caagccacca agaaaaaatt accgctatta gaggtattga catttttaaa agttctaata
      721 aacattccat aattatcact tcctttcact aggagataac taaattatac acaacacaaa
      781 aataaaaagg aggaaaagat atgatgaaaa atagtttgca agctaaagaa cttgcggtaa
      841 ttttatctgt ttctaaatcc aaagcaggac aaataataag agaactgaat aaagagcttg
      901 aagacgaagg gtacattgcg atacgaggca gaatacccgt ccaattagct aggaaaaaat
      961 tcccttatca cgacttatca gaccagagaa taatagagga gttgaagaaa gtaaatgagt
     1021 aaaacttata aaagctatct attagcagta ttgtgcttca cagtcttagc gattgtgctt
     1081 atgccattg
//
LOCUS       DAOBBH010000335         1089 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.337, whole genome shotgun sequence.
ACCESSION   DAOBBH010000335 DAOBBH010000000
VERSION     DAOBBH010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.337"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..1030
                     /locus_tag="P6L01_002670"
     CDS             <1..1030
                     /locus_tag="P6L01_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NEAT domain-containing protein"
                     /protein_id="HDP3031680.1"
                     /translation="EKLKAEYKKKLEDTKKALDEQVKSAITEFQNVQPTNEKMTDLQD
                     TKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYWKDFMVEGQRVRTI
                     SKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQYHVRIVDKEAFTKANTDKSN
                     KKEQQDNSAKKEATPATPSKPTPSPVEKESQKQDSQKDDNKQLPSVEKENDASSESGK
                     DKTPATKPTKGEVESSSTTPTKVVSTTQNVAKPTTASSKTTKDVVQTSAGSSEAKDSA
                     PLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNKDMTLPLMALLALSSIVAF
                     VLPRKRKN"
BASE COUNT          481 a          178 c          172 g          258 t
ORIGIN      
        1 tgaaaaatta aaggctgagt acaagaagaa attagaggat acaaagaaag ctttagatga
       61 gcaagtgaaa tcagctatta ctgaattcca aaatgtacaa ccaacaaatg aaaaaatgac
      121 tgatttacaa gatacaaaat atgttgttta tgaaagtgtt gagaataacg aatctatgat
      181 ggatactttt gttaaacacc ctattaaaac aggtatgctt aacggcaaaa aatatatggt
      241 catggaaact actaatgacg attactggaa agatttcatg gttgaaggtc aacgtgttag
      301 aactataagc aaagatgcta aaaataatac tagaacaatt attttcccat atgttgaagg
      361 taaaactcta tatgatgcta tcgttaaagt tcacgtaaaa acgattgatt atgatggaca
      421 ataccatgtc agaatcgttg ataaagaagc atttacaaaa gccaataccg ataaatctaa
      481 caaaaaagaa caacaagata actcagctaa gaaggaagct actccagcta cgcctagcaa
      541 accaacacca tcacctgttg aaaaagaatc acaaaaacaa gacagccaaa aagatgacaa
      601 taaacaatta ccaagtgttg aaaaagaaaa tgacgcatct agtgagtcag gtaaagacaa
      661 aacgcctgct acaaaaccaa ctaaaggtga agtagaatca agtagtacaa ctccaactaa
      721 ggtagtatct acgactcaaa atgttgcaaa accaacaact gcttcatcaa aaacaacaaa
      781 agatgttgtt caaacttcag caggttctag tgaagcaaaa gatagtgctc cattacaaaa
      841 agcaaacatt aaaaacacaa atgatggaca cactcaaagc caaaacaata aaaatacaca
      901 agaaaataaa gcaaaatcat taccacaaac tggtgaagaa tcaaataaag atatgacatt
      961 accattaatg gcattactag ctttaagtag catcgttgca ttcgtattac ctagaaaacg
     1021 taaaaactaa taaatcgtct ttatatttaa ttattaaatt aacaaatttt aattggcgga
     1081 tgaggtatc
//
LOCUS       DAOBBH010000336         1068 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.338, whole genome shotgun sequence.
ACCESSION   DAOBBH010000336 DAOBBH010000000
VERSION     DAOBBH010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.338"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..123
                     /locus_tag="P6L01_002671"
     CDS             <1..123
                     /locus_tag="P6L01_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HDP3031681.1"
                     /translation="TGTDNNYTIDLSKRLPSTDANRYVKKPQNAKIEVILEKSN"
     gene            231..947
                     /locus_tag="P6L01_002672"
     CDS             231..947
                     /locus_tag="P6L01_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL12"
                     /protein_id="HDP3031682.1"
                     /translation="MSKNITKNIILTTTLLLLGTVLPQNQKPVFSFYSEAKAYSIGQD
                     ETNINELIKYYTQPHFSFSNKWLYQYDNGNIYVELKRYSWSAHISLWGAESWGNINQL
                     KDRYVDVFGLKDKDTDQLWWSYRETFTGGVTPAAKPSDKTYNLFVQYKDKLQTIIGAH
                     KIYQGNKPVLTLKEIDFRAREALIKNKILYTENRNKGKLKITGGGNNYTIDLSKRLHS
                     DLANVYVKNPNKITVDVLFD"
BASE COUNT          421 a          153 c          162 g          332 t
ORIGIN      
        1 acaggtacag acaataacta cacaatagat ttaagtaaaa ggttgccatc aactgatgca
       61 aatagatatg ttaaaaaacc tcaaaatgca aaaattgaag ttatcctcga aaaatcaaac
      121 taacaataat aatggagtta ataaaaataa tcgcaaatac tatattgact tcgctcacat
      181 ttaaatttct tattcctcgt atcatgattc ctctgaaagg agatgttcta atgagtaaga
      241 acatcacgaa aaatataatt ttaacgacaa cattattact attaggtact gtattacctc
      301 aaaatcaaaa accagtattt agtttttact ctgaagctaa agcttatagc attggtcaag
      361 atgaaactaa catcaatgaa ttaattaaat attacacaca gcctcatttt tcattttcaa
      421 ataaatggct atatcaatat gataatggaa acatttatgt tgaacttaag agatattcat
      481 ggtcagcaca tatatcttta tggggcgctg aaagttgggg aaatattaat cagttaaaag
      541 atcgttacgt agatgtgttt ggactaaaag acaaagatac tgatcagtta tggtggtctt
      601 atagagagac atttacaggt ggcgttacac cagccgcaaa accttctgat aaaacttata
      661 atctttttgt gcaatacaaa gataaactac aaacgattat tggtgcgcat aaaatatacc
      721 aaggcaataa accagtatta acattgaaag aaatcgattt ccgtgcacga gaagcgttaa
      781 taaaaaataa aatattatat accgaaaatc gtaataaagg taagcttaag atcaccggtg
      841 gcggtaataa ctacactatt gatttaagca aaagattaca ttcagatcta gcaaatgttt
      901 atgttaaaaa tcctaataaa ataactgttg acgtcctctt tgattagtat atgaaggtga
      961 cttatacttc atgcacttta attccaaatc agattattta aatgataatt tttaaagtgt
     1021 atgatgtata taataggtga aattttctat atatttaaat ggaattgg
//
LOCUS       DAOBBH010000337         1061 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.339, whole genome shotgun sequence.
ACCESSION   DAOBBH010000337 DAOBBH010000000
VERSION     DAOBBH010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.339"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            20..241
                     /locus_tag="P6L01_002673"
     CDS             20..241
                     /locus_tag="P6L01_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cyclic bacteriocin"
                     /protein_id="HDP3031683.1"
                     /translation="MLKLHKKIAWTGIKGSAITTFITALYNGSSVWNALAVAGIAFGG
                     GAGTAVAALGRATVMRFIKRWGVRKTAAW"
     gene            279..>1061
                     /locus_tag="P6L01_002674"
     CDS             279..>1061
                     /locus_tag="P6L01_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031684.1"
                     /translation="MGKPLFKKGDSMNNSIFYTYLKFNLRYFIRRKFEIVKRSRLNVT
                     TKILFLIKLIFSSLLLFISTIMLADYFSDYIDFIEYYFYASMFIYIVFIPLIPRTKLL
                     INPMDKQLLYRSSLREVDIFNLIYASDVLKKFVGYFNLLIVGMAISLYHISPFIFNLK
                     VFCALIYFSTISYVIQIIFINIKVISTKKLFSYHYCVFNFMMGSIVSVIGFVVSFLLV
                     NLLIKPFLVFSTYVIKSKKTFEWYSYFQDIKNEVIIINEKVMQ"
BASE COUNT          345 a          122 c          172 g          422 t
ORIGIN      
        1 agaaggaggt ggaaggccta tgctaaaatt acataaaaaa atagcttgga caggaatcaa
       61 aggttcagct ataactacat ttattactgc cttatataat ggaagtagtg tttggaacgc
      121 gctagctgtt gctggtattg cttttggtgg tggcgctggt actgcggttg cagctcttgg
      181 tcgtgcaaca gtaatgagat ttatcaaacg ttggggcgta cgaaaaacag ctgcttggta
      241 aaagataatc taattcttta taagagaggc tttctttttt gggaaagcct ctttttaaaa
      301 aaggagattc aatgaataat tcaatatttt atacctattt aaaatttaat cttagatatt
      361 ttatccgtag aaaatttgaa attgtaaaaa ggtcccgact taatgttact acaaaaatac
      421 tgtttttaat taaattgatt ttttcgtcac tattattatt tatttcaact attatgttag
      481 ctgactattt cagtgattat attgatttta tagaatatta tttctatgcg agtatgttta
      541 tttacattgt atttatacca ttaataccta gaactaaatt attgatcaat ccaatggata
      601 aacagctgtt atatagatct agtttgagag aagttgacat ttttaacttg atatatgctt
      661 cagacgtttt aaaaaagttt gttggatatt ttaatttgtt gatagttggt atggctatta
      721 gtttatatca tattagtcct tttatattta acctcaaggt tttttgtgca ctaatatact
      781 tttcaacaat ctcttatgta attcaaatta tttttataaa tattaaagtc ataagcacaa
      841 aaaaattatt ttcttatcac tattgtgttt ttaattttat gatgggaagc attgtttctg
      901 ttattggatt tgtcgtctca tttcttttag taaatttatt aataaagccc tttttagttt
      961 tttcgacata tgtaataaaa agtaagaaaa cttttgaatg gtatagttat tttcaggata
     1021 taaagaacga agtcattatt attaatgaaa aagttatgca a
//
LOCUS       DAOBBH010000338         1051 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.340, whole genome shotgun sequence.
ACCESSION   DAOBBH010000338 DAOBBH010000000
VERSION     DAOBBH010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.340"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            154..>1051
                     /locus_tag="P6L01_002675"
     CDS             154..>1051
                     /locus_tag="P6L01_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDP3031685.1"
                     /translation="MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVG
                     YTVDSHKFCQNVADQGCKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQ
                     LVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
                     KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHA
                     KSLLFSQLGEDLSKEKYVVLNNDDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESL
                     QGVSFDFVTPFGTYPVKSPYVGK"
BASE COUNT          376 a          144 c          212 g          319 t
ORIGIN      
        1 attgtaaaac taattcataa taccgtatgt tttattttta atagtagaaa ttagaaaatg
       61 ctgattagta ggatataaca gtgaaattat aaatttatta acatcaacaa aacgtgtata
      121 ataaacatat tgtagaaaaa ggagcggttc agtttggatg caagtacgtt gtttaagaaa
      181 gtaaaagtaa agcgtgtatt gggttcttta gaacaacaaa tagatgatat cactactgat
      241 tcacgtacag cgagagaagg tagcattttt gtcgcttcag ttggatatac tgtagacagt
      301 cataagttct gtcaaaatgt agctgatcaa gggtgtaagt tggtagtggt caataaagaa
      361 caatcattac cagctaacgt aacacaagtg gttgtgccgg acacattaag agtagctagt
      421 attctagcac acacattata tgattatccg agtcatcagt tagtgacatt tggtgtaacg
      481 ggtacaaatg gtaaaacttc tattgcgacg atgattcatt taattcaaag aaagttacaa
      541 aaaaatagtg catatttagg aactaatggt ttccaaatta atgaaacaaa gacaaaaggt
      601 gcaaatacga caccagaaac agtttcttta actaagaaaa ttaaagaagc agttgatgca
      661 ggcgctgaat ctatgacatt agaagtatca agccatggct tagtattagg acgactgcga
      721 ggcgttgaat ttgacgttgc aatattttca aatttaacac aagaccattt agattttcat
      781 ggcacaatgg aagcatacgg acacgcgaag tctttattgt ttagtcaatt aggtgaagat
      841 ttgtcgaaag aaaagtatgt cgtgttaaac aatgacgatt cattttctga gtatttaaga
      901 acagtgacgc cttatgaagt atttagttat ggaattgatg aggaagccca atttatggct
      961 aaaaatattc aagaatcttt acaaggtgtc agctttgatt ttgtaacgcc ttttggaact
     1021 tacccagtaa aatcgcctta tgttggtaag t
//
LOCUS       DAOBBH010000339         1047 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.341, whole genome shotgun sequence.
ACCESSION   DAOBBH010000339 DAOBBH010000000
VERSION     DAOBBH010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.341"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(82..165)
                     /locus_tag="P6L01_002676"
     tRNA            complement(82..165)
                     /locus_tag="P6L01_002676"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(129..131),aa:Leu,seq:caa)
     gene            complement(204..278)
                     /locus_tag="P6L01_002677"
     tRNA            complement(204..278)
                     /locus_tag="P6L01_002677"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(243..245),aa:Gly,seq:tcc)
     gene            complement(382..456)
                     /locus_tag="P6L01_002678"
     tRNA            complement(382..456)
                     /locus_tag="P6L01_002678"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Gly,seq:tcc)
     gene            complement(464..537)
                     /locus_tag="P6L01_002679"
     tRNA            complement(464..537)
                     /locus_tag="P6L01_002679"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(503..505),aa:Cys,seq:gca)
     gene            complement(543..614)
                     /locus_tag="P6L01_002680"
     tRNA            complement(543..614)
                     /locus_tag="P6L01_002680"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(580..582),aa:Gln,seq:ttg)
     gene            complement(626..698)
                     /locus_tag="P6L01_002681"
     tRNA            complement(626..698)
                     /locus_tag="P6L01_002681"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(663..665),aa:His,seq:gtg)
     gene            complement(701..774)
                     /locus_tag="P6L01_002682"
     tRNA            complement(701..774)
                     /locus_tag="P6L01_002682"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(740..742),aa:Trp,seq:cca)
     gene            complement(790..870)
                     /locus_tag="P6L01_002683"
     tRNA            complement(790..870)
                     /locus_tag="P6L01_002683"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(834..836),aa:Tyr,seq:gta)
     gene            complement(876..951)
                     /locus_tag="P6L01_002684"
     tRNA            complement(876..951)
                     /locus_tag="P6L01_002684"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(916..918),aa:Thr,seq:tgt)
     gene            complement(959..1031)
                     /locus_tag="P6L01_002685"
     tRNA            complement(959..1031)
                     /locus_tag="P6L01_002685"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(996..998),aa:Phe,seq:gaa)
BASE COUNT          314 a          245 c          239 g          249 t
ORIGIN      
        1 attgtataga gagagaaata aaaagaaacc ttgttttaca aggtttctaa tacgttatgt
       61 tatgtaaata acagttaatt ataccggtgg tcggggtcga accgacactc cacaagtgga
      121 acgggatttt gagtcccgcg cgtctgccaa ttccgccaca ccggcttaat ggtaaacaaa
      181 aaacttccct ttggaagcaa ttatggagcg gaagatagga tttacaccta tacctcgttc
      241 cgggaaggaa cgtgttctaa aagttgaact actcccgcaa atattaaatt atggagcgga
      301 agataggatt tacacctata cctcattcca ggaaggaatg tattctaaga gttgaaatac
      361 tcccgcatta ttattaaatt atggagcgga agataggatt tgcacctata cctcgttccg
      421 ggaaggaacg tgttctaaaa gttgaactac tcccgcataa acctggaggc ggcaaccgga
      481 tttgaaccgg tgataaaggt tttgcagacc tctgccttac cacttggcta tgccgccaat
      541 aactgggcta gctggattcg aaccaacgag tgacggagtc aaagtccgtt gccttaccgc
      601 ttggctatag cccattaata ataagggcgg ctgaagggga tcgaaccctc gaatgtcgga
      661 accacaatcc gatgtgttaa ccacttcacc acagccgcca tggcaggggc agtaggaatc
      721 gaacccacac caaaggtttt ggagacctct attctaccgt tgaactatgc ccctattaaa
      781 aataataaat ggaggggggc agattcgaac tgccgaaccc gaaggagcgg atttacagtc
      841 cgccgcgttt agccacttcg ctacccctcc ataaatggtg ccggccagag gacttgaacc
      901 cccaacctac tgattacaag tcagttgctc taccaattga gctaggccgg ctaagaaatg
      961 gttcaggaca gagtcgaact gccgacacat ggagcttcaa tccattgctc taccaactga
     1021 gctactgaac cataataaaa atgtaat
//
LOCUS       DAOBBH010000340         1040 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.342, whole genome shotgun sequence.
ACCESSION   DAOBBH010000340 DAOBBH010000000
VERSION     DAOBBH010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.342"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            118..909
                     /locus_tag="P6L01_002686"
     CDS             118..909
                     /locus_tag="P6L01_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
                     /protein_id="HDP3031686.1"
                     /translation="MHDNTIKSISKDYFDYLQTESKFIPLENETIEFYSPIVDYFGDS
                     ISVNISFSGDRYKLTDHGETLWNMEQLGIDLTSNKKQKKYQLLNNIVQNYSLVIENKH
                     INFYTNRINLSQAIHDYVLAISEISYLGILKKENIKSLFKDEVMQYFLSNRKIYPNIF
                     PEFKVEGKSKLIHSFEAVFPGEATEYVKTIKRIDKNNAKNVLFDWNDVEIHRNKNFDS
                     NARLNIITEQQDDISEAVSTMLSQYNVEVFSFENKKQLEEKFSNV"
BASE COUNT          415 a          138 c          141 g          346 t
ORIGIN      
        1 tatttgacga tagctctgat tttgtaaaac aattatttac tttgcttgaa tatacaaaaa
       61 ctaatcataa tgataatatt aaaattgaaa ctaatctatt catgagttgg tgatactatg
      121 catgacaaca caattaaatc tattagcaaa gactattttg attacttaca aacagaatca
      181 aagtttatcc cattagaaaa tgaaactata gaattttatt cacctatcgt cgattatttt
      241 ggtgattcta tttctgtaaa tatatctttt tcaggggatc gatataaact aactgatcat
      301 ggtgaaactt tatggaacat ggaacaatta ggtatagatt taactagtaa taaaaaacaa
      361 aaaaagtatc aacttctcaa taatattgta caaaactaca gtctagtcat cgaaaataag
      421 cacataaact tttatacaaa tagaataaac ttatctcaag ctattcatga ttatgttttg
      481 gctatttccg agatcagtta tttgggaata ttaaagaaag aaaatattaa gtccttattt
      541 aaagacgaag tgatgcaata ttttctatca aacagaaaga tttatcctaa tatatttccc
      601 gaatttaagg tagaggggaa atctaagtta atccatagtt tcgaagcagt atttccagga
      661 gaagctacag agtatgttaa aacaattaaa cgaatcgata aaaacaatgc aaaaaatgtc
      721 cttttcgatt ggaatgatgt tgaaatacat agaaataaaa attttgattc taatgctaga
      781 cttaatatca ttacagaaca acaagatgat attagcgaag cagtatcaac aatgttgtct
      841 caatataatg tagaggtatt ttcatttgaa aacaaaaaac aacttgaaga aaagtttagt
      901 aacgtataat tatcgtgtta tataatgttt cttcgataat agtttctatt tcaattaaat
      961 caccgatact taaaatgata aatttaccaa taccaagttt gtaatgaact ttcaccgtca
     1021 tacaatcata gatgctatat
//
LOCUS       DAOBBH010000341         1025 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.343, whole genome shotgun sequence.
ACCESSION   DAOBBH010000341 DAOBBH010000000
VERSION     DAOBBH010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.343"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            118..>1025
                     /locus_tag="P6L01_002687"
     CDS             118..>1025
                     /locus_tag="P6L01_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDP3031687.1"
                     /translation="MIKLIQISNINKSFNKRCVLKNISFDIEQGKCIALIGKNGAGKS
                     TLIDILIGNVNANSGEIFDKDKLLQSENRSIMFQKTMFPDQLKVIEIINLYQSFYENP
                     LPLEEIIELTKFDSSQLNQFVNKLSGGQQRLLDFVLSLIGQPQLILLDEPTSTMDIEI
                     REYFWSIIENLKEDNRTILYTSHYIEEVERMSDKIILIENGEIILNDSTSHIRTNQQS
                     QITLSDEYIRKLKLDKDDLVIQKNHNGTIKIITSNVNDTILYLQQLHINLDDIEIQKV
                     SIVDSYFNNKKQRGSNYDTKLLENRI"
BASE COUNT          422 a          127 c          139 g          337 t
ORIGIN      
        1 taacgataaa tatattatgt aaacctaata atgacatttg tcatttcaaa tctatgacaa
       61 agtgttctat tttaaatcta ataaatttac taatattaaa ttaagaaaaa aatagaggtg
      121 attaaattga ttcaaatatc taatatcaac aagtcattta ataaaagatg tgttctaaaa
      181 aatatttcgt tcgatattga acaaggtaaa tgtatcgctt taattggaaa aaatggtgct
      241 ggaaagtcaa cgttaattga tatattaatt ggtaatgtta atgctaattc tggtgagata
      301 tttgataaag acaagttatt acaaagtgaa aatcgcagta taatgttcca aaaaacgatg
      361 tttccagatc aattaaaagt tattgagatt atcaacttat atcaatcatt ttacgaaaat
      421 ccattaccat tggaagaaat aatagaactg acgaaatttg attctagtca actgaaccaa
      481 tttgtaaata aactttctgg tggtcaacaa cgattactcg attttgtatt atctttaatc
      541 ggacaaccac aattgatctt attagatgaa ccaacatcga ctatggatat agaaattaga
      601 gaatattttt ggtcaattat tgaaaattta aaagaagata atcgaacgat actctataca
      661 tcgcactata ttgaagaagt cgaacgtatg tcagacaaaa ttattctcat tgaaaatgga
      721 gaaataatac ttaatgattc aacgtcacat attagaacca atcagcaatc tcagattacg
      781 ttatccgatg aatatataag aaagttaaaa ctagataaag atgatttagt tattcaaaaa
      841 aatcataatg gcactatcaa aattattact tcaaatgtaa atgatacgat tttatatctt
      901 caacaacttc atattaattt ggatgatatt gaaatacaaa aagtctcaat tgttgattca
      961 tacttcaaca ataaaaagca aaggggatct aattatgata ctaagttact tgaaaatcga
     1021 attta
//
LOCUS       DAOBBH010000342         1025 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.344, whole genome shotgun sequence.
ACCESSION   DAOBBH010000342 DAOBBH010000000
VERSION     DAOBBH010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.344"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..201
                     /locus_tag="P6L01_002688"
     CDS             <1..201
                     /locus_tag="P6L01_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDP3031688.1"
                     /translation="FFGYGISKLPYLLYPFVKITDAYVNPEMGWTLVIVFILGLLLLL
                     PSLILLLRLFVFDKEYVEGKKS"
     gene            334..996
                     /locus_tag="P6L01_002689"
     CDS             334..996
                     /locus_tag="P6L01_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDP3031689.1"
                     /translation="MGKEYVVIGLGRFGGSIVRELNALDMDVMAIDHDENRVNEYSDI
                     ATHAVVADTTDEAVMKSLGIRNFDHVIVAIGENIQSSTLTTLILKELGVKKVTAKAQN
                     DYHAKILNKIGADTVVHPERDMGRRIAHNVASASVLDYLELADEHSIVELKATEKMAG
                     QSIIDLDIRAQYGINIIAIKRGKEFIISPNPNINLEIGDILIMIGHDNDLNRFEKNIA
                     TR"
BASE COUNT          346 a          125 c          213 g          341 t
ORIGIN      
        1 ttctttggct atggaattag taaattgcca taccttttat atccgtttgt aaaaattaca
       61 gatgcatatg ttaatccaga aatgggctgg acattagtga ttgtctttat tttaggttta
      121 cttttattac ttccatcgtt aatattatta ttaagattat ttgttttcga caaagaatat
      181 gttgaaggaa agaaatcata agttgagacg ataccccaac tttcatttac ttgcattact
      241 gcatttagat aatttagttg gggtactcat ttataataat tttaaattta tattttcaat
      301 gaattgatga aaatcattta atggagggcg agtatgggta aagaatatgt agtcatcggt
      361 ctaggtcgtt ttggaggtag tatcgttcgt gagttgaatg cattagacat ggatgtaatg
      421 gccatcgacc atgatgaaaa tagagtgaat gaatatagtg atatcgctac tcatgcggtt
      481 gttgcagaca caacagatga agcagttatg aaaagtttag gtatccgtaa ctttgatcat
      541 gtcattgtgg caattggtga aaatattcaa tcaagtacgt tgacgacttt aattttaaaa
      601 gagttaggtg taaaaaaagt aactgctaaa gcacaaaatg attatcatgc aaagatttta
      661 aataaaattg gagcagatac ggttgtgcac cctgagcgtg atatgggtag acgtattgcg
      721 cataatgttg cgagtgcaag tgtacttgat tatcttgagt tggcagacga gcattctatt
      781 gtagaattga aagcaactga aaagatggcg gggcagtcta tcattgattt agatataaga
      841 gcacaatatg gaattaacat tattgcaatt aaacgaggca aagagttcat catttcacca
      901 aatccaaata ttaatttaga aataggtgat attttaatca tgattggaca tgataatgat
      961 ttaaatcgct ttgaaaaaaa tattgcgacg agataatcgg gagtgggaca gaaatgatat
     1021 tttct
//
LOCUS       DAOBBH010000343         1020 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.345, whole genome shotgun sequence.
ACCESSION   DAOBBH010000343 DAOBBH010000000
VERSION     DAOBBH010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.345"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(621..854)
                     /locus_tag="P6L01_002690"
     CDS             complement(621..854)
                     /locus_tag="P6L01_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031690.1"
                     /translation="MVFNDWEINVVYSGEHEVVTNEKSLFVIDEFYDVAIAIYYLDGK
                     LKVAHVNYGSDFTIDVANKVFELNIQNPNVDEH"
BASE COUNT          375 a          132 c          116 g          397 t
ORIGIN      
        1 atgttgactc cttataatat atgtttaatt cattaaaata gttgaaaaca tgacttgaaa
       61 taaagatata aagctaaagt aaggatgttc aattgtttca aattttatga aagtataacg
      121 ttaaatatgt taatttgatg atgatatttg ctttttattt tccaaatgga atttacttaa
      181 actgatgcat taaaatatta atgaagcact agaatacata aatgaatagt aatggtgcac
      241 agtatagaat aattaaggct atattaagta taaatatcgt taactgtaag ctatctttag
      301 ttttaatata aactattagg ataatcgacg taagaagaat catatatatt aatgatgaag
      361 aagtccatac aaaatccgca tcatttgttg ttaataatgg gactataatt aatccgaaat
      421 taatcatgca tgctatatat actataatgt tatacacaat gttaattttt gttcaccacc
      481 ttatacttct attttaaaaa cttctttata atgatatatt gtttaatgtt gaaataatta
      541 gattatctaa ttttcatttg ctttacatgt aaaaggctat atatagtatg ctctttatga
      601 ttctaaatgc tttttaatat ttaatgctca tcaacatttg gattttgaat attcaattca
      661 aaaactttat tagctacgtc aattgtaaaa tcagaaccat agttgacatg agctactttt
      721 aattttccat ctaaataata gattgcgatt gcaacatcgt aaaattcgtc aatgacaaat
      781 aaactctttt cgtttgttac aacctcatgc tctcctgagt atacaacgtt aatttcccaa
      841 tcattaaaaa ccatttgtta acctccttga acatttaaat tgattcaact taagtttaac
      901 ttattcatac aacttcgtac aatatctaga tgaacattaa ttgtatttct agaaatcttt
      961 ttcaattata tgtactaatt atacttttaa atttcttatt tcagtatagt tttaaaacga
//
LOCUS       DAOBBH010000344          990 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.347, whole genome shotgun sequence.
ACCESSION   DAOBBH010000344 DAOBBH010000000
VERSION     DAOBBH010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.347"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..330
                     /locus_tag="P6L01_002691"
     CDS             <1..330
                     /locus_tag="P6L01_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDP3031691.1"
                     /translation="FSSGMKQKLNFASMLLVDSNVLLLDEPFNALDHVAQKNFTRILK
                     GLVSKEKIIIFTSHLPNTILELSENIYLLKDGCFTDKRKAQSFNQTTLETWLLESQKE
                     VIPYEEK"
     gene            496..693
                     /locus_tag="P6L01_002692"
     CDS             496..693
                     /locus_tag="P6L01_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDP3031692.1"
                     /translation="MKNNLKIARVSLSMTQKELANKVGVTRQTISLIEKGVHNPSLSL
                     CKNICSVLNKNLDEIFGEKPQ"
BASE COUNT          406 a          115 c          143 g          326 t
ORIGIN      
        1 ttttcaagtg gtatgaagca aaaactaaat tttgcttcta tgttattagt agattcaaat
       61 gttttattat tagatgaacc atttaatgcc cttgaccatg ttgctcaaaa aaatttcaca
      121 cgaattttaa agggattagt ttctaaagaa aagataataa ttttcactag tcatttgcct
      181 aatacaattt tagaactctc agaaaatatt tatttgttaa aagatggatg ttttacagat
      241 aaacgaaaag cacagtcatt taatcaaacc acgttagaaa catggttgtt agaaagtcaa
      301 aaagaggtga taccttatga agaaaaatga tttgttagta atgagtgtag tatatatttt
      361 aacttcagtc ataatgttct acattaatgg attaagtaaa caatttttta catatgtagt
      421 tggtttccca attatttatt ttggttatat atgtctagtt aatcttataa aaaaaagaag
      481 ccggaataat taaaagtgaa aaataatttg aaaatagcta gagtttcact atctatgact
      541 caaaaggagc tagcaaataa agttggagta actagacaaa caatatctct tatagaaaag
      601 ggtgtacata acccctctct atcactatgt aaaaatattt gttcggtgtt gaataaaaat
      661 ttggatgaga tatttggaga gaaaccacaa taaaagagct tattgaaaga taatctaaaa
      721 gtcgacaata tatttcttct cagaaactgc tattattttg aaaagaaaac acacgtatta
      781 taaatttaaa ataccgaaat atagcattat taaatcaaaa atagaattaa aaaagaaaat
      841 taatcgagaa aagtattaaa tgtcatttat ttgaatgtgt taattatgaa agaaaaagag
      901 taattgatgt taaatataca ttgactaagc taagttgaca cactcgaaaa tctgaatatt
      961 ctaataaaat ataaataatc ccccgcactt
//
LOCUS       DAOBBH010000345          988 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.348, whole genome shotgun sequence.
ACCESSION   DAOBBH010000345 DAOBBH010000000
VERSION     DAOBBH010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.348"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..67)
                     /locus_tag="P6L01_002693"
     CDS             complement(<1..67)
                     /locus_tag="P6L01_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HDP3031693.1"
                     /translation="MNNTLTIDQLQELLQIQKEFDD"
     gene            complement(60..230)
                     /locus_tag="P6L01_002694"
     CDS             complement(60..230)
                     /locus_tag="P6L01_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031694.1"
                     /translation="MKKFNVQITYAGMIEETIEAESLEEAEIEADFIAIFEASFNYDE
                     YEINVEEAQENE"
     gene            complement(217..471)
                     /locus_tag="P6L01_002695"
     CDS             complement(217..471)
                     /locus_tag="P6L01_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HDP3031695.1"
                     /translation="MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKVQAFDEILK
                     GLPNAMQDALKEDIGLDEAVGIMTGQVVYKYEEEQENEEI"
     gene            complement(464..835)
                     /locus_tag="P6L01_002696"
     CDS             complement(464..835)
                     /locus_tag="P6L01_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDP3031696.1"
                     /translation="MKVNYETGFQLGVMEARLKKMRKQRDACKKQRDELIVDIAKLRE
                     RNEELENMWRTVKNELLGRYEFYRFRLNELQIESRANKAVAINMGAKINASAILYRMD
                     KLDGTNEFYEFLGQMEDDTNE"
     gene            complement(844..>988)
                     /locus_tag="P6L01_002697"
     CDS             complement(844..>988)
                     /locus_tag="P6L01_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031697.1"
                     /translation="NGTYYFHGHIVPGWQSVKKTFDTAEELEIYIKQHGLEYEEQKEL
                     TLF"
BASE COUNT          240 a          243 c          128 g          377 t
ORIGIN      
        1 tatcgtcgaa ctccttttgt atttgtaata actcttgtaa ttgatcaatt gttaatgtgt
       61 tattcatttt cctgtgcctc ctctacatta atttcatatt catcataatt aaatgatgct
      121 tcaaatatcg caataaaatc cgcctcaatt tctgcttctt ctaaactttc agcctcgata
      181 gtctcttcaa tcatgccagc gtatgtgatt tggacattaa atttcttcat tttcctgctc
      241 ctcctcatat ttatagacca cttgacccgt cataatccct actgcctcat caagaccaat
      301 atcttctttg agtgcatctt gcatagcatt aggtaaaccc ttaagtattt catcaaacgc
      361 ttgtactttc ttatacacgt cctcaatctc ttttagtaat ccctctgtgt cattgccgtt
      421 atacgcacta gcacttataa cggactgttc tatttgttca cggttattca ttagtgtcat
      481 cctccatttg tcctaaaaat tcgtagaact catttgttcc gtctaatttg tccattcggt
      541 acaatatagc acttgcgttg attttagctc ccatgtttat agctactgcc ttgttcgctc
      601 tactctcaat ctgtagttcg ttaagtctaa aacggtaaaa ttcgtatctt ccaagcaatt
      661 catttttgac tgtgcgccac atgttctcca gctcttcgtt acgctctctt aacttagcta
      721 tatccacgat aagctcatca cgttgcttct tgcacgcatc acgttgtttt ctcatcttct
      781 tcaacctagc ttccattaca cctagttgga accctgtttc atagttcact ttcataacct
      841 cctctaaaat aaagttagtt ccttctgttc ttcgtattcc aaaccatgtt gctttatata
      901 tatttcgagc tcttcagcag tatcaaatgt ctttttaaca ctttgccaac ctggcacgat
      961 atgcccatga aagtaataag tgccgttt
//
LOCUS       DAOBBH010000346          981 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.349, whole genome shotgun sequence.
ACCESSION   DAOBBH010000346 DAOBBH010000000
VERSION     DAOBBH010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.349"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..185
                     /locus_tag="P6L01_002698"
     CDS             <1..185
                     /locus_tag="P6L01_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDP3031698.1"
                     /translation="GYINENKEDDFVAYGSPEHNYQFGGSLIKSKNLSTLLKPVHQTK
                     SPDEIKKELESKKNDR"
     gene            452..862
                     /locus_tag="P6L01_002699"
     CDS             452..862
                     /locus_tag="P6L01_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDP3031699.1"
                     /translation="MSKKKILILTSIMLIILISVASIYFKMKYDEKEKQKSIYYKEQQ
                     ARITLYLKHNTIEPNTIKSVHFTKLETSPMGSAVIEGYINENKEDDFVAYASPENNFQ
                     FVGDLIKSERLSELLKPAHQLKSPDDIKKELNKK"
BASE COUNT          392 a          123 c          147 g          319 t
ORIGIN      
        1 aaggttacat aaatgaaaat aaagaagatg attttgttgc atatgggtct ccagaacata
       61 attatcaatt tggtggaagt ttaatcaaaa gtaaaaattt aagcacgtta ttaaaaccag
      121 tacatcaaac caaatcacct gatgagataa aaaaggaatt agagtctaaa aagaacgacc
      181 gctaaaacta tgcggccgtt ttaattttgt tttcaagtta atcatactta catataaaaa
      241 tgaaaagttg aatttttagt acaatttcca ctgtatttat ctgttaacca ctttataccc
      301 gcaatatttc gttgatctta tataaacact tttggctgct tttccttgac ctagattaaa
      361 attatagatt ttctttgtaa gagtgtgttt tgtagtatca taaatatgta aactgtataa
      421 aattccacca cattttagga gctggtgata gatgtcgaaa aagaaaattt taatattaac
      481 tagtattatg ttaatcatat taattagtgt tgcaagtatc tattttaaaa tgaaatatga
      541 cgaaaaagaa aaacaaaaat caatttatta taaagaacaa caagcgcgca ttacacttta
      601 tcttaagcat aatactatag aaccgaacac aatcaaatct gtacatttca caaaattgga
      661 aacaagtcct atgggaagtg ctgtgattga aggttacata aatgaaaata aagaagatga
      721 ttttgttgcc tatgcatcac ctgaaaataa ctttcaattt gtaggtgatt taattaaaag
      781 tgaaagatta agtgagttac taaaaccagc gcatcaatta aaatcaccag atgatataaa
      841 aaaagaacta aataaaaagt agagtcacta aagttaggag ttactttaat gaacaaaaaa
      901 catgtttttg taattattgg tgtcatttta tgtatatgta tagttgcatc agtcatttat
      961 ttaaaagtga aatatgacga a
//
LOCUS       DAOBBH010000347          980 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.350, whole genome shotgun sequence.
ACCESSION   DAOBBH010000347 DAOBBH010000000
VERSION     DAOBBH010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.350"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(289..831)
                     /locus_tag="P6L01_002700"
     CDS             complement(289..831)
                     /locus_tag="P6L01_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'(3')-deoxyribonucleotidase"
                     /protein_id="HDP3031700.1"
                     /translation="MTRKSIAIDMDEVLADTLGEIIDAVNFRADLGIKMEALNGQKLK
                     HVIPEHDGLITEVLREPGFFRHLKVMPHAQEVVKKLTEHYDVYIATAAMDVPTSFSDK
                     YEWLLEFFPFLDPQHFVFCGRKNIVKADYLIDDNPRQLEIFTGTPIMFTAVHNINDDR
                     FERVNSWKDVEQYFLDNIEK"
BASE COUNT          307 a          197 c          133 g          343 t
ORIGIN      
        1 tcccataaga aatactaatt tctaaagaaa aagtatttct ttatgttggg gcccacccca
       61 acttgcattg tctgtagaaa ttggcgttcc aatttctcta tgttggggcc ccgccaactt
      121 gcattgtctg tagaaattgg gaatccaatt tctctatgtt ggggccccgc agtcaactac
      181 tgccaatata gtgttgtagt gtctaagaca taaaagttca tctcagtcac aattacttta
      241 tagcctatcc atcatctttt ctacatgaaa tttttcaagt gatatatttt atttctcaat
      301 attatctaaa aaatactgtt ctacatcttt ccagctattt acgcgttcaa atcgatcatc
      361 attaatatta tgcactgctg taaacataat cggtgtacca gtaaaaattt caagctgtct
      421 aggattgtca tctattaaat aatcagcttt aacgatgttt tttctaccac aaaaaacaaa
      481 atgctgagga tctaaaaatg gaaagaactc tagtaaccat tcatatttat cactaaatga
      541 tgttggtaca tccattgctg ctgtagcaat atatacatca taatgttcag ttaatttttt
      601 cacaacttct tgtgcatgtg gcatcacttt aagatgtctg aagaagcctg gttctctcaa
      661 tacttctgta attaatccat catgttcagg aataacatgt ttaagttttt gaccattcaa
      721 agcttccatt ttaataccta aatccgctct aaaattgaca gcatcaatga tttctcctaa
      781 tgtatctgcc aatacttcat ccatatcaat cgcgattgat ttacgggtca ttttaatctt
      841 ctcctcaaaa tatcagtatt gattcagtat agcaaaaaag caattgatac aaaacaatat
      901 catctattgt tgctttttac actgaatact ctcacctcat cattcacact gcattgagct
      961 tgagacataa atcaatgttc
//
LOCUS       DAOBBH010000348          978 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.351, whole genome shotgun sequence.
ACCESSION   DAOBBH010000348 DAOBBH010000000
VERSION     DAOBBH010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.351"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            17..664
                     /locus_tag="P6L01_002701"
     CDS             17..664
                     /locus_tag="P6L01_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDP3031701.1"
                     /translation="MKFDNYIFDFDGTLADTKKCGEVATQSAFKACGLTEPSSKEITH
                     YMGIPIEESFLKLADRPLDEAALAKLIDTFRHTYQSIEKDYIYEFAGITEAITSLYNQ
                     GKKLFVVSSKKSDVLERNLSAIGLNHLITEAVGSDQVSAYKPNPEGIHTIVQRYNLNS
                     QQTVYIGDSTFDVEMAQHAGVPSAAVTWGAHDARSLLHSNPDFIINDPSEINTVL"
BASE COUNT          366 a          125 c          178 g          309 t
ORIGIN      
        1 aataggggtg ttaatattga agtttgacaa ttatattttt gattttgatg gtacgttggc
       61 agacacgaaa aaatgtggtg aagtagcaac acaaagtgca tttaaagcat gtggcttaac
      121 ggaaccatca tctaaagaaa taacgcatta tatgggaata cctattgaag aatcattttt
      181 aaaattagca gaccgaccat tagatgaagc agcattagca aagttaatcg atacatttag
      241 acatacatat caatctattg aaaaggacta tatttatgaa tttgcgggta taactgaagc
      301 cattacaagt ttgtataacc aagggaaaaa acttttcgtg gtgtctagta agaagagtga
      361 tgtattagaa agaaatttat cggctattgg attaaatcac ttgattaccg aagctgttgg
      421 atccgatcaa gtaagtgcat ataaaccaaa tcctgaaggc atacacacaa ttgtgcaacg
      481 ctacaattta aatagccaac aaacggttta tattggtgat tcaacatttg atgttgagat
      541 ggcgcaacat gctggtgtac catctgcagc agtaacatgg ggagcacatg atgcaaggtc
      601 attacttcat tcaaatccag attttataat caatgatcca tcagaaatta atacagtatt
      661 ataaaatttg ttaaaacaga gaataccatg gttaaaatgc atattcataa atattagatt
      721 atacatagaa atatttcgct ttagattagg aatgtaaaat aaatatttat taataatcgt
      781 gtatgtttaa agagtgatgt ctgattcgtt gatcatttat ttttgtacaa ataaattaaa
      841 gtaaaaacaa agttattgaa gtaaatcgaa tataaacatt ttaatatgat gaggaaagta
      901 gtgatagcat atgacttaca tgaagttgtg tgctatcgct atttttagtt ataattccaa
      961 aaagttaatc gttcgatg
//
LOCUS       DAOBBH010000349          976 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.352, whole genome shotgun sequence.
ACCESSION   DAOBBH010000349 DAOBBH010000000
VERSION     DAOBBH010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.352"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            239..338
                     /locus_tag="P6L01_002702"
                     /pseudo
     CDS             239..338
                     /locus_tag="P6L01_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501275.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(347..958)
                     /locus_tag="P6L01_002703"
     CDS             complement(347..958)
                     /locus_tag="P6L01_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDP3031702.1"
                     /translation="MEQIITEFISRFGYAAIFILILLENVLPIVPSEIILTFAGLMSV
                     KSHLSILTLFIIATIASFIGLLILYYICRLISEERLYRFIDRHGKWIKLKSKDLKRAN
                     DWFKKYGVWAVFICRFIPVLRVLITIPAGVNRMNVVTFTVISLIGTTIWNFGLILLGR
                     TLSDSFGMLMTGLHTYSRIMYVVIIIAVIYFAIRYIGKRKRVK"
BASE COUNT          363 a          150 c          159 g          304 t
ORIGIN      
        1 attaaaatgc gcaatttgtt atgagtaact tttttggata gtaggcacat taaagtgtcg
       61 aaaacaattt gtgtggagga cagggtaaat tctgtttatt taagatgata gtttgtatta
      121 tagatggatt tgtgagataa gtttgcggaa tgtatacata ctgagggaac tgaactttga
      181 tgggaaactt cattatatct cttaacgtaa aggattttag gtgactttca gctaaagtat
      241 gccaaataaa cttaaaaaag cagtgaatca tttcaataga tggaatgatt ttactgcttt
      301 ttgtgtatag aaatttaaaa ttcatgcctt tatactaaca acaatattat tttactctct
      361 tacgtttgcc aatgtaacgt atagcaaagt aaatgactgc gataatgatg acaacataca
      421 taatacgtga atatgtatga agaccagtca tcaacatacc aaagctatcg ctcaacgtgc
      481 gtcctagtaa aattaaaccg aaattccaaa ttgttgtacc tattaatgaa ataacggtaa
      541 atgtcacaac attcatgcgg tttacaccgg ctggaatggt aatcaataca cgtaatacag
      601 gtatgaaacg acagataaat acagcccata cgccatactt tttaaaccaa tcatttgctc
      661 gctttaaatc cttacttttc aatttaatcc acttaccgtg tcgatcaata aaacgatata
      721 gacgttcttc tgaaatcaaa cggcagatat aatataaaat taacagccct ataaacgatg
      781 cgatggttgc aataataaat aaggttaaaa ttgataaatg tgatttaaca gacataaggc
      841 cagcaaatgt cagaataatt tccgatggta cgataggtaa aacattttct aataaaatta
      901 atataaaaat ggctgcatac ccaaaacgac taataaattc agtgataatt tgttccatta
      961 ttaaaaataa gctcct
//
LOCUS       DAOBBH010000350          944 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.353, whole genome shotgun sequence.
ACCESSION   DAOBBH010000350 DAOBBH010000000
VERSION     DAOBBH010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.353"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            26..904
                     /locus_tag="P6L01_002704"
     CDS             26..904
                     /locus_tag="P6L01_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HDP3031703.1"
                     /translation="MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGVPTNGIQGFVR
                     HIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQF
                     GFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIY
                     NRYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVV
                     ENIDALSAGQRNKINDNLDELYLSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSIC
                     NEHELHVSGKYISSHF"
BASE COUNT          352 a          130 c          164 g          298 t
ORIGIN      
        1 tattaagaaa gaaggaacgt tttaaatgcc taataaaata ttacttgtag atggtatggc
       61 gctattattt agacatttct atgctacaag tcttcataaa caatttatgt acaattcaca
      121 aggtgtacct acaaatggaa tacaaggatt tgtgcgtcat atcttttcgg caatacatga
      181 aatacgccct acacatgtag ctgtatgttg ggatatggga caatcaactt ttagaaatga
      241 tatgtttgat ggttataagc aaaatcgttc tgcaccacca gaagaattga taccacaatt
      301 tgattatgtt aaagaaattt cagagcaatt tggctttgta aatattggcg ttaaaaacta
      361 tgaagcggat gatgttatag gtacattagc acaacaatat tcaactgata acgatgtcta
      421 tattattacg ggcgacaaag atttactgca atgtattaat gacaatgttg aagtctggct
      481 aattaaaaaa ggttttaaca tttataatag atatacatta catcgtttta acgaagaata
      541 tgcccttgaa ccacaacaac taatcgatat taaagcgttt atgggtgata cagcagatgg
      601 atatgcaggt gttaaaggaa ttggtgagaa aacggctatt aagttaattc agcaatatca
      661 aagcgttgaa aatgtggttg aaaacattga tgcattatca gctgggcaac gtaataagat
      721 taatgacaat ttagatgaac tatatttatc gaagcgactt gctgaaattc acacacaagt
      781 accaattgat agtgaagcgc tttttgagaa aatgtcattt gcaacaacac ttaatcatat
      841 tttatcaatt tgtaacgaac atgaattaca tgtttcagga aaatatattt catcccattt
      901 ttaaaaaagt ataaaatttt atgaaaaatt aaaccaatta agta
//
LOCUS       DAOBBH010000351          903 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.354, whole genome shotgun sequence.
ACCESSION   DAOBBH010000351 DAOBBH010000000
VERSION     DAOBBH010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.354"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            187..618
                     /locus_tag="P6L01_002705"
                     /pseudo
     CDS             187..618
                     /locus_tag="P6L01_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086052.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            655..>903
                     /locus_tag="P6L01_002706"
     CDS             655..>903
                     /locus_tag="P6L01_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
                     /protein_id="HDP3031704.1"
                     /translation="MTTFDAKKLKKEYLDWYNQILEFSNLSNNSVKIDTPFKDNSLDN
                     LKIYALYDQSRDMITLTDDGYTIFGLENNGVSINKFKKR"
BASE COUNT          365 a          110 c          135 g          293 t
ORIGIN      
        1 ctatgaaaat gttgatgaac tacatcttta aatcttatta tacattgtat aaaattatat
       61 tgcgaggtac taaattgatg atatacactt ttggttgtgt atgcatctca cctatctttt
      121 tttgcttttt tcaaataaga aagtattaaa aattaatgcc tatacaacgt taaaggagga
      181 acagacatgg ctaaacttga tttaaatagt cttgacgatg agcacgtaaa attattaata
      241 aatgaattaa aatatccaga aactcatatc gatgtaaatg aataaaaaac aatagttgct
      301 agtcgaataa atgaaaggca agaaataata agttttaagt taggaataaa gtacttctta
      361 acaataaaaa gggataacat agaaaaagat aggttttcaa tttcaatcat tttcaaagat
      421 acctatcaca ccctagttag aatagatatt aacggtggta ctcacgataa tccagatgga
      481 acaatcgctc cgaaaagtca tattcacata tataatgata agtgtgataa aaaggatagg
      541 tttgcttatg aaattaactt gaaagatttc cccgacatct ataacttgta taatgtgtat
      601 atgtcgtttt tagagtaaaa aaatataaaa gaccttgaat aaaggaggga taatatgact
      661 acgtttgatg ctaaaaaatt aaaaaaagaa tatcttgatt ggtataatca gattttagag
      721 ttttctaatt tatcaaacaa ttcagtaaaa atagataccc cttttaaaga taattcttta
      781 gataatttaa aaatatacgc tttatacgat cagtccagag acatgattac actgactgat
      841 gacggctata ctatatttgg tttagaaaat aacggtgttt ctataaataa atttaaaaaa
      901 cgt
//
LOCUS       DAOBBH010000352          844 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.355, whole genome shotgun sequence.
ACCESSION   DAOBBH010000352 DAOBBH010000000
VERSION     DAOBBH010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.355"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(20..514)
                     /locus_tag="P6L01_002707"
     CDS             complement(20..514)
                     /locus_tag="P6L01_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDP3031705.1"
                     /translation="MIEIKTLTNNDFNEYKRLVSTVNEEFTQDSHYSQTMTDTLIHDI
                     LNQGSPKCIVFGCYENETLIATAALEQIRYVGKEHKSLIKYNFVTNNDKSINSELINF
                     IINYARQNNYESLLTSIVSNNIGAKVFYSALGFDILGFEKNAIKIGNTYFDEHWLFYD
                     LINK"
BASE COUNT          292 a          117 c          122 g          313 t
ORIGIN      
        1 tagaatatat aaaactgtat tacttattaa tcaaatcata aaaaagccaa tgttcatcga
       61 aataggtatt tccgatttta attgcatttt tctcaaaacc aagaatgtcg aatcctagtg
      121 cactatagaa aactttagca cctatgttgt ttgacacaat tgatgtaagt aaagattcgt
      181 aattgttctg ccgtgcataa ttaataatga aattaatgag ctcgctatta atcgatttat
      241 cgttattagt aacaaagttg tatttaatta atgatttatg ttcttttcca acgtatcgaa
      301 tttgttctaa ggcagctgtt gcgataagtg tttcgttttc ataacagcca aatacaatac
      361 atttcggtga accttgattt aaaatgtcat gtattaaggt gtcagtcatt gtttgactat
      421 aatgtgaatc ttgagtgaat tcttcattga ctgtcgaaac aagtctctta tactcattaa
      481 aatcattatt cgttaatgtt ttaatttcaa tcattactga cctccttaat cattattaag
      541 aaagattttg tactaaaaaa atttcttaat tacatattaa aatgtttatt aaaattttgc
      601 aatcactgat ttatatgcac attcttgtaa atggattatc aatttaatta tatttatttt
      661 taagttcagg aaattatgga attggttatt gatcgacact ggacttttat ttggagacgt
      721 tatgcgaaga agtcaagcca gtattggaac cggtcacatt tatgatgact aagggacaat
      781 cattttcagt taaatattgc cagaaatttc agtcacgaat agaaaaaata accaaccaaa
      841 aatt
//
LOCUS       DAOBBH010000353          843 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.356, whole genome shotgun sequence.
ACCESSION   DAOBBH010000353 DAOBBH010000000
VERSION     DAOBBH010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.356"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            43..579
                     /gene="amaP"
                     /locus_tag="P6L01_002708"
     CDS             43..579
                     /gene="amaP"
                     /locus_tag="P6L01_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDP3031706.1"
                     /translation="MKRLKNFILGLLIVVIVGFLLFMYIQDGRITEYQDYFLQFEWFQ
                     PLLISLAALLILIGLILVFSIFKPTHRKPGLYKDFDDGHVYVSRKAVEKTAFDTVAKY
                     DQVRQPNVVAKLYNKKNKSYIDIKTDFFVPNNVQVQSLTEAIRSDIKKNVEYFTEMPV
                     RKLEVNVRDQKTSGPRVL"
     gene            592..831
                     /locus_tag="P6L01_002709"
     CDS             592..831
                     /locus_tag="P6L01_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDP3031707.1"
                     /translation="MANNHNQNGQDSTQQVINFLKVFKWRIVGFLAFLLIAILFLTLG
                     FWKTVLIIVLCLIGVGIGYMKDRKQDFMNFLNRWS"
BASE COUNT          288 a          121 c          149 g          285 t
ORIGIN      
        1 tatgttttta aattaacttt aactaaggag gtgtcgttag gggtgaaacg acttaaaaac
       61 tttatccttg gcttattaat agtagtaatc gtggggttcc tgttattcat gtatatccaa
      121 gatggacgta taactgaata tcaagactac ttccttcaat ttgaatggtt ccaaccatta
      181 cttatttcac ttgctgcact attgatatta ataggtctta tattagtatt tagtatcttc
      241 aaacctacgc atcgaaaacc tggattatac aaagacttcg atgatggtca tgtttacgta
      301 tctcgtaaag ctgttgaaaa gacggcattc gatacggttg caaaatatga tcaagtaaga
      361 caaccaaatg tagttgcgaa actatataac aaaaaaaata aatcttatat cgacattaaa
      421 actgactttt tcgtaccaaa taatgttcaa gttcaatctt taactgaagc aattcgttcg
      481 gatattaaaa agaatgtaga atatttcaca gaaatgccag tacgtaagtt agaagttaat
      541 gtgagagatc agaaaacttc tggtccacga gtgttgtaag ggaggtatat catggctaac
      601 aatcataacc aaaacggaca agactctaca caacaagtga ttaatttttt aaaagtattt
      661 aaatggagaa tcgttggttt cctagcgttt ctattaatcg cgatattatt cttaacgtta
      721 ggtttttgga aaacagtact tatcatcgtt ttatgtttaa ttggtgtagg tattgggtat
      781 atgaaagacc gtaagcaaga ttttatgaat tttttaaatc gatggagtta attcatcaat
      841 tta
//
LOCUS       DAOBBH010000354          838 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.357, whole genome shotgun sequence.
ACCESSION   DAOBBH010000354 DAOBBH010000000
VERSION     DAOBBH010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.357"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..192
                     /locus_tag="P6L01_002710"
     CDS             <1..192
                     /locus_tag="P6L01_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDP3031708.1"
                     /translation="FTSEGKLKVSLDYIDWINTEFDQLGRENYYMYKKFGVIPEMEYE
                     MEEIKEIEQYIKEQDEAEL"
     gene            203..664
                     /locus_tag="P6L01_002711"
     CDS             203..664
                     /locus_tag="P6L01_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001668280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDP3031709.1"
                     /translation="MTFEEKLSQMYNEIANKISSMIPVEWEKVYAMAYVNERSGEVFY
                     NYTEPRSDELFYYTSVLNKYNISRSEFMDSVYELYKQFDKLRDLFKEEGLEPWTSCEF
                     DFTRDGKLNVSFDYIDWANSEFGQMGREHYYMYKKFGIWPEKEYAINWVKK"
     gene            713..>838
                     /locus_tag="P6L01_002712"
     CDS             713..>838
                     /locus_tag="P6L01_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HDP3031710.1"
                     /translation="MNFEEKLSQMYNEIANEISGMIPVEWEQVFTIAYVTDQAGEV"
BASE COUNT          346 a           79 c          178 g          235 t
ORIGIN      
        1 tttacaagtg aaggtaaatt aaaagtttca cttgattata ttgattggat aaatacagag
       61 tttgatcaat taggccgtga aaattattat atgtacaaaa aatttggggt tataccagaa
      121 atggaatatg aaatggaaga aattaaagaa atcgagcaat atattaaaga gcaagatgaa
      181 gctgaactat aggggcgata atatgacttt cgaagaaaaa ctaagtcaaa tgtacaatga
      241 aattgcaaat aaaattagca gtatgatacc ggtagaatgg gaaaaggtat atgcaatggc
      301 ttatgtaaat gaaagaagtg gagaagtgtt ctacaattat actgaaccaa gaagtgatga
      361 attattttac tatacgagcg tgttaaataa atataatata tcaagatcag aatttatgga
      421 ctcagtatat gaattatata agcaatttga taaactaaga gatttattta aagaagaagg
      481 actcgaacca tggacatcat gtgaatttga ctttacaaga gacggcaaat tgaatgtttc
      541 atttgattat attgattggg cgaattcaga gtttggacaa atgggaagag aacattatta
      601 catgtataaa aaatttggaa tttggcctga aaaagaatat gccataaatt gggtaaaaaa
      661 ataaaagatt atgttaaaga gcaagatgaa gctgaactat aggggcgata acatgaattt
      721 tgaagaaaaa ttaagtcaaa tgtacaacga gattgcgaat gagattagtg ggatgatacc
      781 agtagaatgg gagcaagtat ttacaatagc ttatgtaact gatcaagctg gggaagtc
//
LOCUS       DAOBBH010000355          830 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.358, whole genome shotgun sequence.
ACCESSION   DAOBBH010000355 DAOBBH010000000
VERSION     DAOBBH010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.358"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..208
                     /locus_tag="P6L01_002713"
     CDS             <1..208
                     /locus_tag="P6L01_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HDP3031711.1"
                     /translation="KNNDYNVKQLRKRYDISTEKAPKLLLKGTGDLKGSSIGHKDIEF
                     TFVENQEENIFFTDSLEFTSSENY"
     gene            325..830
                     /locus_tag="P6L01_002714"
                     /pseudo
     CDS             325..830
                     /locus_tag="P6L01_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269697.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
BASE COUNT          346 a           90 c          165 g          229 t
ORIGIN      
        1 gaaaaataat gattataatg taaagcaatt acgtaaaaga tatgatatat caacagaaaa
       61 ggctcccaaa ttgttgttga aaggtactgg agatttaaaa ggttcatcaa tcggacataa
      121 ggacattgag tttacattcg tggagaatca agaagagaat atatttttta ctgatagttt
      181 ggagtttacc tcaagtgaga actattagct atgataaaaa aggggatacg gaacaatgaa
      241 taattttaga aaatgtgcgt tgtgtatagg acgtcagtct taattctatt agcatcaggt
      301 tgcagtggcg tatttgacac tcctgaagat tcaaaagaaa cacaaatcaa aaagagcttt
      361 gcgaaaacgt tagatatgta tccaattaaa aatcttgagg atttatacga taaagaagga
      421 tatcgagatg gagaatttaa aaagggagac aaagggacgt gggttgttag atctgaaatg
      481 ataattcaac caaaaggtaa aagtttaact tcaagaggca tgatattata catgaataga
      541 aatactagga ccacaaccgg ttatttttca atagaagaaa tagattcaag aaaaagcctt
      601 gatgaaagag aaacagaaaa aaagtatcct gtgaaaatga taaacaataa aattattcca
      661 actgaggaga taaaagatga aaagttgaaa aaggaaattg aaaactttaa gttttttgtg
      721 caatatggca gttttaaagg aatagagaat tatgaaaatg gtgacatttc ctataattct
      781 gaagctccta tttattcagc gaaatataaa ctgaaaaatg atgattataa
//
LOCUS       DAOBBH010000356          809 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.359, whole genome shotgun sequence.
ACCESSION   DAOBBH010000356 DAOBBH010000000
VERSION     DAOBBH010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.359"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(145..279)
                     /locus_tag="P6L01_002715"
     CDS             complement(145..279)
                     /locus_tag="P6L01_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031712.1"
                     /translation="MHYLICIKANQRLIFTNSIVDWFYLCMNEQLFDIIKNINDFESI
                     "
     gene            complement(342..>809)
                     /gene="splF"
                     /locus_tag="P6L01_002716"
     CDS             complement(342..>809)
                     /gene="splF"
                     /locus_tag="P6L01_002716"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplF"
                     /protein_id="HDP3031713.1"
                     /translation="EIKAHPNGFYNNGGGLYKVTKIVDYPGKEDIAVVQVEEKSTQPK
                     GRKFKDFTSKFNIASEAKENEPISVIGYPNPNGNKLQMYESTGKVLSVNGNIVSSDAI
                     IQPGSSGSPILNSKHEAIGVIYAGNKPSGESTRGFAVYFSPEIKKFIADNLDK"
BASE COUNT          251 a          112 c          126 g          320 t
ORIGIN      
        1 ataagtaatt tatctctttg tgtaatagat ttattataac atttggttat gttgcgatgt
       61 tgataatttg gatgttggtg gtggaaattt tgagttttag tggcgcaatt gttattgaaa
      121 aattttatag aaatgttgta gctttcaaat gctttcaaaa tcatttatat tcttaatgat
      181 gtcaaaaagt tgttcattca tacataaata aaaccaatca acaattgagt tggtgaaaat
      241 caatcgttga ttggctttga tgcatattaa ataatgcaat atattaataa atgttagtta
      301 tagtatattt tgtcaggatt gggtgaatgt ctaagtttta attatttatc taaattatct
      361 gcaatgaatt tcttaatttc aggagagaaa taaacagcaa atcctcttgt gctttcacct
      421 gatggcttat taccggcata gattacacca atagcttcgt gtttactatt taatataggt
      481 gaaccagagc taccaggctg aataattgca tccgatgaca ctatattccc attcactgat
      541 aatactttac cagttgattc atacatttgt agtttatttc cattaggatt tggataacca
      601 atgactgata taggttcatt ttctttagct tctgatgcta tattaaattt actagtgaaa
      661 tctttgaatt ttctaccttt tggttgtgtt gatttttctt caacttgtac aaccgcaata
      721 tcttctttac caggataatc tacaatctta gtaactttat aaagtccacc accgttatta
      781 taaaaaccat taggatgtgc tttgatttc
//
LOCUS       DAOBBH010000357          797 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.361, whole genome shotgun sequence.
ACCESSION   DAOBBH010000357 DAOBBH010000000
VERSION     DAOBBH010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.361"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(30..545)
                     /locus_tag="P6L01_002717"
     CDS             complement(30..545)
                     /locus_tag="P6L01_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDP3031714.1"
                     /translation="MKINTEFKGNNIPYEYAAGADISDLINGNPIKSFPFEITELPKD
                     TKYLAWSLIDYDAIPVCGFAWIHWSVANVSITGDSISIKADLSRTKGDYVQGKNSFTS
                     GLLAEDFSEIENHYVGPTPPDKDHQYELTVYALDHSLNLKNGFYLNEFLKEVNQHKID
                     QTSINLIGRKI"
     gene            complement(523..>797)
                     /locus_tag="P6L01_002718"
     CDS             complement(523..>797)
                     /locus_tag="P6L01_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031715.1"
                     /translation="ISMIFLGISSGLTRPQTINKLSSSSNLRVMLNYAETLYFIFNIA
                     FLLIGGYLYSIGTIQYLMLFMSLLTFIYLLTLFYLRRDQHENQHRI"
BASE COUNT          277 a          124 c          103 g          293 t
ORIGIN      
        1 acaattttat attgatgaga tatttagcat taaatttttc ttcctataag gttaatactt
       61 gtttgatcaa ttttatgttg atttacttct tttaaaaatt cattcaagta gaacccattc
      121 ttcaaattta aagaatgatc taacgcataa actgttaatt catattgatg atctttatca
      181 ggtggtgttg gtcctacata gtgattttct atttctgaaa aatcttcagc caacaaccca
      241 ctagtaaagc tatttttacc ttgtacatag tcaccctttg ttcttgataa atctgctttt
      301 atagaaattg aatcaccagt gatacttaca ttagcaacgc tccaatgaat ccatgcaaat
      361 ccgcaaactg gtattgcatc ataatctatc agagaccatg caagatattt agtatctttt
      421 ggaagttctg ttatttcaaa cgggaatgat ttaattggat tgccatttat taaatcactt
      481 atatctgcgc ctgccgcata ttcatatggt atattatttc ctttaaattc tgtgttgatt
      541 ttcatgttga tctctcctta aataaaataa tgttagtaaa tatataaacg tcaataatga
      601 cataaataac attaaatatt gaatagttcc aattgaatat agataaccac caataagtaa
      661 aaatgcgata ttaaaaataa aatataacgt ttctgcataa ttaagcatca ctcttaagtt
      721 actactgcta gaaagtttat ttatagtttg tggacgagtt aaccctgaag aaatacctaa
      781 aaatatcata gaaatag
//
LOCUS       DAOBBH010000358          769 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.362, whole genome shotgun sequence.
ACCESSION   DAOBBH010000358 DAOBBH010000000
VERSION     DAOBBH010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.362"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..276
                     /locus_tag="P6L01_002719"
     CDS             <1..276
                     /locus_tag="P6L01_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020807974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDP3031716.1"
                     /translation="KNNLNFKKGMKQARKGVGYKAAIEVHTTLFSRSSNFSKDKKLDD
                     VLDLSESTKKLHLNFENTKIFLQLAKSTISTNRVNYSDNESIRIEVE"
     gene            334..>769
                     /locus_tag="P6L01_002720"
     CDS             334..>769
                     /locus_tag="P6L01_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDP3031717.1"
                     /translation="MFKRIKLILIATIILSGCSTTNNESNNETKSVPEEMEASKYVGQ
                     GFQPPAEKDAIEFAKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVEVYVHCEDH
                     GIVFNASLPLYKDAIHQKGSMRSNDNGDDMSMMVGTVLSGFEY"
BASE COUNT          308 a          101 c          149 g          211 t
ORIGIN      
        1 aaaaataatc tgaattttaa aaaaggtatg aaacaagcaa ggaaaggagt aggctataaa
       61 gctgcaatag aagtacatac aacattgttt tcgagaagta gtaacttttc aaaggacaaa
      121 aaattagatg atgttttaga tttgtctgaa agtacgaaaa agttacacct taattttgaa
      181 aatacgaaaa tatttttaca actagcaaaa tctactatta gcactaatcg agttaattac
      241 agtgataatg agtctataag gattgaggtc gaatgacttg aaatcagcta atttctctat
      301 attctaaaca aacacataaa tgggagatgg gctatgttta aaagaattaa actaatctta
      361 atagcaacga taatactatc aggatgttca actaccaata acgaatccaa caatgaaaca
      421 aaatcagtac cagaagaaat ggaagcttca aaatatgtag gacaaggctt ccaaccacct
      481 gcagaaaaag atgcgattga atttgcgaag aagcatcgta aagaatttga aaaagtaggt
      541 gaacaattct ttaaagataa ctttggacta aaagttaaag ctacaaatgt tgtaggtaaa
      601 gatgatggtg tagaagttta tgtgcattgc gaagatcatg gcattgtatt taatgcaagt
      661 ctacctttgt acaaagatgc catccatcaa aaaggatcaa tgcgcagtaa tgacaatggt
      721 gatgatatga gtatgatggt gggtacagtg ctgagtggct ttgaatatc
//
LOCUS       DAOBBH010000359          767 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.363, whole genome shotgun sequence.
ACCESSION   DAOBBH010000359 DAOBBH010000000
VERSION     DAOBBH010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.363"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            116..>767
                     /locus_tag="P6L01_002721"
     CDS             116..>767
                     /locus_tag="P6L01_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HDP3031718.1"
                     /translation="MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSST
                     VAPIVEKLNEKWAQDHSDAKISAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQ
                     DKNIKYKEFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKI
                     NAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQ"
BASE COUNT          305 a           97 c          156 g          209 t
ORIGIN      
        1 acaaattttt atgtaacatt tacagtttct aaaaatgagg ttaataattc aaggttaaga
       61 taaagatgta atcaatacaa atactatttg ttgttcatac agggaggata tttcaatgaa
      121 aaaatggcaa tttgttggta ctacagcttt aggtgcaaca ctattattag gtgcttgtgg
      181 tggcggtaat ggtggcagtg gtaatagtga tttaaaaggg gaagctaaag gtgatggctc
      241 atcaacagta gcaccaattg tggagaaatt aaatgaaaaa tgggctcaag atcactcgga
      301 tgctaaaatc tcagcaggac aagctggtac aggtgctggt ttccaaaaat tcattgcagg
      361 agatatcgac ttcgctgatg cttctagacc aattaaagat gaagagaagc aaaaattaca
      421 agataagaat atcaaataca aagaatttaa aattgcgcaa gatggtgtaa cggttgctgt
      481 aaataaagaa aatgattttg tagatgaatt agacaaacag caattaaaag caatttattc
      541 tggaaaagct aaaacatgga aagatgttaa tagtaaatgg ccagataaaa aaataaatgc
      601 tgtatcacca aactcaagtc atggtactta tgacttcttc gaaaatgaag taatgaataa
      661 agaagatatt aaagcagaaa aaaatgctga tacaaatgct atcgtttctt ctgtaacgaa
      721 aaacaaagag ggaatcggat actttggata taacttctac gtacaaa
//
LOCUS       DAOBBH010000360          766 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.364, whole genome shotgun sequence.
ACCESSION   DAOBBH010000360 DAOBBH010000000
VERSION     DAOBBH010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.364"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..75)
                     /locus_tag="P6L01_002722"
     CDS             complement(<1..75)
                     /locus_tag="P6L01_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 family protein"
                     /protein_id="HDP3031719.1"
                     /translation="MSNIYKSYLVAVLCFTVLAIVLMPL"
     gene            complement(72..392)
                     /locus_tag="P6L01_002723"
     CDS             complement(72..392)
                     /locus_tag="P6L01_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HDP3031720.1"
                     /translation="MPHVLNVTVPIPETHVLITKDEYDELIGYSLDPVWNMSDLKKKL
                     KIASDETIKDRLLFHPRFEKELRAQGIVHYPDENFNRWRFNARKMNKFVDEHFNEIYK
                     ERIK"
     gene            complement(422..640)
                     /locus_tag="P6L01_002724"
     CDS             complement(422..640)
                     /locus_tag="P6L01_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031721.1"
                     /translation="MNALYKTTLLTTMAVVTWKVWKIEKHTRKPVISIRALSDYLNNK
                     SLTIPKDAENSTESARRLLKFAEQTISK"
BASE COUNT          209 a          138 c          124 g          295 t
ORIGIN      
        1 caatggcata agcacaatcg ctaagactgt gaagcataat actgctacta ggtagctttt
       61 ataaatgttg ctcattttat tctctcctta tatatttcat tgaaatgctc atcgacgaat
      121 ttattcatct ttcttgcgtt aaatctccag cgattaaaat tctcatctgg gtaatgcaca
      181 attccttgcg ctcttagttc tttttcaaat ctaggatgaa atagtaatct gtccttgata
      241 gtctcatcag atgcaatttt taatttcttc tttaagtcac tcatgttcca tacagggtct
      301 aatgaataac caattagctc atcatattca tcttttgtga taagtacatg tgtttcaggt
      361 attggaacgg ttacgtttaa tacatgtggc atttctatca ttcctttcgt gtataatgtt
      421 gttatttgct aatagtttgt tcggcgaact tcaaaaggcg acgagcagat tcagtagaat
      481 tttcagcatc tttcggtatg gttaaagatt tgttgtttag atagtcactc aacgccctga
      541 tactaatcac aggttttcta gtgtgcttct caatcttcca aaccttccac gtcacaactg
      601 ccattgtggt gaggagggtt gttttgtata gtgcgttcat ttgtttatgc tcctttgcat
      661 ttccaaaaat ttaatataat ttaaattcga taccatctat ttgaatgtat agattatcta
      721 aatcagggat tgccttttta tataaaccaa atcttgattt gatatc
//
LOCUS       DAOBBH010000361          761 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.365, whole genome shotgun sequence.
ACCESSION   DAOBBH010000361 DAOBBH010000000
VERSION     DAOBBH010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.365"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            102..671
                     /locus_tag="P6L01_002725"
     CDS             102..671
                     /locus_tag="P6L01_002725"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDP3031722.1"
                     /translation="MKAKYKNHQIETNEKRIKNHLNRAFNREQPMETLVSDLTYVKVG
                     GTWHYICLFIDLFNREIVGYSAGKNKDANLVSKAISRINHNLEQIKLFHTDRDKEFDN
                     HLIDEVLETFKIKRSLSTKGCPYDNAVAEATMKAMKTEFVKQMQFENLEQLETELFDY
                     VNWYNNFRPHSSLQYLTPVAFKNIHMKTV"
BASE COUNT          325 a          106 c          116 g          214 t
ORIGIN      
        1 tctttagtac aagacgaatt aaaaatcatt taaatgacaa gggtctcact gtatctggac
       61 aaaagatagg tcgattatga aaaaatctag tttctgttta tatgaaagct aaatacaaaa
      121 atcatcaaat agaaactaat gaaaaacgaa ttaaaaatca tttgaatcgc gcttttaata
      181 gagaacaacc aatggagaca ttagtaagtg atttgacata tgtaaaagtc ggaggaacat
      241 ggcattacat atgtttattt atagatctat ttaatagaga aattgttggt tacagtgcag
      301 gtaaaaataa ggacgccaat ttagtatcaa aagcaatcag cagaataaat cataatcttg
      361 aacaaatcaa actattccac actgatagag acaaagaatt tgataatcat ttgatagatg
      421 aagtcctaga aacatttaaa ataaaacgtt cattaagtac caaaggttgt ccttatgata
      481 acgcagttgc ggaagcaacg atgaaagcaa tgaaaacaga atttgtaaaa caaatgcaat
      541 ttgaaaactt agaacagtta gaaacagaat tatttgatta tgtaaattgg tacaacaatt
      601 ttagaccaca ttcttcatta cagtatttga cgccagtggc atttaaaaat atacacatga
      661 aaactgtcta aaaaatgttg acataccact aagtgaacta ttaaaaccaa aagaaattta
      721 atacaattca agaccatgca aactcattta taaacattgt c
//
LOCUS       DAOBBH010000362          746 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.366, whole genome shotgun sequence.
ACCESSION   DAOBBH010000362 DAOBBH010000000
VERSION     DAOBBH010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.366"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            240..344
                     /locus_tag="P6L01_002726"
     CDS             240..344
                     /locus_tag="P6L01_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031723.1"
                     /translation="MFKVNYSILSYYPEYNIAVSWQRLREGKTIKNKI"
     gene            472..723
                     /locus_tag="P6L01_002727"
     CDS             472..723
                     /locus_tag="P6L01_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031724.1"
                     /translation="MLTLIKLKEDEQVIIYEYIPEDDISNGKGSVTFNKKDAEVIDFS
                     LSEIENEEYFMLYRNKSFSVVRDFIEKQEFPENYKIAWY"
BASE COUNT          300 a           80 c          122 g          244 t
ORIGIN      
        1 gaaggggcat ttccattata ttgttatagc tttttatatt tgtataatga acatataagt
       61 ttaagaagat gagagtgaag gaaataaaaa agctcaaatg taccaaattg ttaatcttaa
      121 taaatctcta ctttataaag attgaatgga cattcgagcg ttaatcagtc aggagggaat
      181 ttcactccta caatttaata ataatacttg cttcaacact gtacaaggag tgatttgtta
      241 tgttcaaagt gaattattcg attttaagtt attatccaga atataatatc gcagtaagtt
      301 ggcaacgttt aagagaagga aaaacaataa aaaacaagat ttaatactgc tgcgtcatga
      361 ggcgcttgaa cattatttga tgaataagta taatttcaac tatgattatg catataaaat
      421 tgtatcaaaa aatacgatta ttcaattttt ataaaaaaga aggtggatta aatgcttaca
      481 ttaataaaat tgaaagaaga tgaacaggtt ataatatatg aatatatacc tgaagatgat
      541 ataagtaacg gtaaaggttc agtaactttt aataaaaaag atgcagaggt tatagatttc
      601 tcattatctg aaatagaaaa tgaagaatat tttatgttat atcgtaataa gtctttttct
      661 gtagtaagag actttatcga gaagcaagaa tttcccgaaa attataaaat agcatggtat
      721 taaagcatcc tttctacaca gataaa
//
LOCUS       DAOBBH010000363          744 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.367, whole genome shotgun sequence.
ACCESSION   DAOBBH010000363 DAOBBH010000000
VERSION     DAOBBH010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.367"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..212)
                     /locus_tag="P6L01_002728"
     CDS             complement(<1..212)
                     /locus_tag="P6L01_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031725.1"
                     /translation="MCIVIYLIYSLIVYFICIINFFIMAAPFLMTNADYVWTPMTTVT
                     LFILSLIIFLIFIKTKDVVHLTIFIL"
     gene            complement(523..702)
                     /locus_tag="P6L01_002729"
     CDS             complement(523..702)
                     /locus_tag="P6L01_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031726.1"
                     /translation="MKFKTTKECKSNNIFKRSQEINNSESEKGCLWGISMLILLFLLI
                     LFGITACSSSIHFIN"
BASE COUNT          308 a          101 c           88 g          247 t
ORIGIN      
        1 tttaatatga aaattgttaa atgaacgaca tcttttgttt ttataaatat caagaaaata
       61 atcaaactca aaataaataa cgtaactgta gtcataggcg tccatacata atcagcatta
      121 gtcattaaga atggtgcggc cattatgaaa aaatttataa tgcagatgaa atagacaatt
      181 agactataaa ttaggtaaat aacaatacac acccttcata aataaataat ttaaatccta
      241 tatattttaa caaaagtaaa acacagaagt gtagaaaata aaaaatattg gtaaataaaa
      301 tcaataagtt taaccaatat gttgctcgct tcataccgta tattgcaaca aaaattccga
      361 tcaagaaaaa tatagcccct atgataaaac agaaatccga tgctgaatta ttaaaaaatg
      421 aggtgtttag agttagaaaa tgagttaatg agttgactat aactaataag atattaatta
      481 tatttgtatg gttcttcaca tgatacctcc aagtaaaaaa atctaattaa taaagtgaat
      541 gcttgatgaa caagcagtta ttccaaacag aatcaataag aaaagtagaa tcaacatgct
      601 aatgccccat aaacaaccct tttcactttc actattatta atttcttgac ttcttttaaa
      661 gatattatta cttttacatt ctttagttgt tttaaatttc acgtttttat tacttccttt
      721 tgtttaaaag tttacaatga attt
//
LOCUS       DAOBBH010000364          729 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.368, whole genome shotgun sequence.
ACCESSION   DAOBBH010000364 DAOBBH010000000
VERSION     DAOBBH010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.368"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            23..715
                     /locus_tag="P6L01_002730"
     CDS             23..715
                     /locus_tag="P6L01_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031727.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKS
                     KNIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEAN
                     DKNAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKN
                     YVEYKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAV
                     CGTAFAAGCSVG"
BASE COUNT          318 a           97 c          125 g          189 t
ORIGIN      
        1 cttattatct tggaggtatc aaatgaaaat tataaaatta ttttcaataa tttccttgtt
       61 cctactggta tttagcgcga atttttctaa tgcttacgca aatgaagaac aaaaaagttc
      121 tttactagaa aatcaaaaag aaaaattgaa aagcaaaaac atcgaagtat cacaaatgac
      181 caagaaagaa ataagttttc tagaacaatt gcaaaataaa aatgaagata tgcaaaaatt
      241 cgaaaagcaa atgaaaaaag aaggatttaa aaaaataaag ctagaagaca aaaattctaa
      301 attaaaatat ctagctacag aagcaaatga taaaaatgca tatggctaca tagctaataa
      361 cgcttataaa aatgaaaaaa caaaagaaat tgtagtttct gaaacaatat atgataacta
      421 caacaaaaaa atcacaaaat tcattgccga aaaaagaagt atgactgaaa acaaagaggg
      481 aaaaatactt attaacaaaa attacgttga atataaggct gaaaaagttg ataaaaacag
      541 accggtaaca tatgcaggat tcaaatggaa cggcaaagca ttcgcttgta gtatggctgg
      601 actttatgca tgtgctcact actgtggagt atgggcaatc gtaaatccaa tagccggcgg
      661 tgtatgtgaa gcagtttgcg gaactgcatt tgcagctgga tgctctgtag gttgatttta
      721 ttccaattg
//
LOCUS       DAOBBH010000365          720 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.369, whole genome shotgun sequence.
ACCESSION   DAOBBH010000365 DAOBBH010000000
VERSION     DAOBBH010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.369"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(437..643)
                     /locus_tag="P6L01_002731"
     CDS             complement(437..643)
                     /locus_tag="P6L01_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031728.1"
                     /translation="MNEIETIISEIEKLLTNNTPYSISKNSGVPRQTVTDLKVGKTKI
                     KEAKFKTIIKLYEYQRTLENKTEC"
BASE COUNT          260 a          104 c           65 g          291 t
ORIGIN      
        1 ttttcaaata aatgtaccaa aaactttcat gaaataaagt atacttttca aatctattaa
       61 ttttggaatc ataaaccaaa tgctatatta caatataatt atcacaaatc atcatatatg
      121 ccaattacac aatttaatat aaaaaattgt caataaaaaa taattcgcac aaacattatt
      181 acaaaatatg tataatatag aatactgcat taaaattaac ttatttactg tgttcagcct
      241 ttaatatcat cacttaaaga ttattcaaga tttaatggca atgataattt cataatatgc
      301 atttttgatt atttaaccat gtacaatttc taaaactaag agattatctt attgaggtgg
      361 acaaaacgtg gacagacaac catgaaatca ttagaaattt aatttcttgt ccacctaacc
      421 taacaaaata tttttgttaa cattctgttt tattttctaa tgttctttga tattcatata
      481 acttgattat cgttttaaat ttagcttctt ttattttagt tttacctacc tttaaatctg
      541 taactgtttg acgtggtaca cctgaatttt ttgaaatact atatggtgta ttgttagtta
      601 ataacttttc tatttcactt ataatagttt caatctcatt catttttaat cacgctctct
      661 ttctatttat aattataata atcctaatgc agtaactacg tttagcaata cattgtttct
//
LOCUS       DAOBBH010000366          715 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.370, whole genome shotgun sequence.
ACCESSION   DAOBBH010000366 DAOBBH010000000
VERSION     DAOBBH010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.370"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
BASE COUNT          295 a           98 c           83 g          239 t
ORIGIN      
        1 tttaacatcg ttttgttgtt ttgaattata aaaactaaag taaaaatgtt ggatgtgaaa
       61 gattgttgat gagaattttg agatgtagat aaatatcaaa ctagtctata ggtaaaaatt
      121 tataattaat ttgaaaagaa tgtctcaaat aatgtttgaa ttagcaggtg tcatactaag
      181 taatgttaag taaacatttt atactttatc agaacaaaaa agacatgcat caacatgtca
      241 gtgaaactaa tccatattat cttaacaacg aaattgaatc gaacctgaaa aacgtattta
      301 caaatcgcaa ctactctatc caaggcgaaa tcgcaaaatc tgatccccga aataacatat
      361 ctcgaaaaaa attccataat tcaatcatct tcaataatta acaatacatt actagttcca
      421 aatatactca tacatacatg tttcattgat tgactgcata ttgtcataaa taatagggga
      481 actaatcgct aataatattg ttaaacacaa tacataataa agtgaaagat tttaaataca
      541 tgaattgttc accattccgc catatatttt caattaccta atttccgaat aaatattaca
      601 attttaattc aataattaat atttaagacc aaaataatcc taaaaagtca attttaattg
      661 cgtgatattg agttttaagc atatttaaat aaatgagtca attcatgtat aatta
//
LOCUS       DAOBBH010000367          714 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.371, whole genome shotgun sequence.
ACCESSION   DAOBBH010000367 DAOBBH010000000
VERSION     DAOBBH010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.371"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            91..>714
                     /locus_tag="P6L01_002732"
     CDS             91..>714
                     /locus_tag="P6L01_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HDP3031729.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGES"
BASE COUNT          269 a          126 c          132 g          187 t
ORIGIN      
        1 tataattgtg taaaagtaaa aaggccatat aacagtcctt ttacggtaca atgtttttaa
       61 cgacaaaaac atacccagga ggacttttac atgacccaag tacattttac actgaaaagc
      121 gaagagattc aaagcattat tgaatattct gtaaaggatg acgtttctaa aaatatttta
      181 acaacggtat ttaatcaact aatggaaaat caacgaacag aatatattca agcaaaagaa
      241 tatgaacgaa cagaaaaccg acaaagtcaa cgaaatggct attatgagcg cagctttacg
      301 acacgtgtag gcacgctaga attaaaagta cccagaacac gtgatggcca tttttcaccc
      361 acagtgtttg aacgttatca acgaaacgaa aaagccctca tggcttcaat gttggaaatg
      421 tatgtatcag gcgtttcaac tcgtaaagta tcaaaaattg tggaagaact ttgtggtaaa
      481 tccgtctcta agtccttcgt ttctagctta acagaacagc tagaacctat ggttaacgag
      541 tggcagaatc gtttattatc agaaaaaaat tatccttact taatgaccga tgtactctat
      601 ataaaagtac gagaagaaaa tcgagtactc tcaaaaagct gtcatatagc gattggaata
      661 accaaagatg gcgaccgtga aattatcggc ttcatgattc aaagtggcga aagc
//
LOCUS       DAOBBH010000368          678 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.373, whole genome shotgun sequence.
ACCESSION   DAOBBH010000368 DAOBBH010000000
VERSION     DAOBBH010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.373"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(38..>678)
                     /locus_tag="P6L01_002733"
     rRNA            complement(38..>678)
                     /locus_tag="P6L01_002733"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          155 a          197 c          148 g          178 t
ORIGIN      
        1 gtaagtaaaa gtgattttgc ttcgcaaaac atttattttg attaagtctt cgatcgatta
       61 gtattcgtca gctccacatg tcaccatgct tccacctcga acctattaac ctcatcatct
      121 ttgagggatc ttataaccga agttgggaaa tctcatcttg aggggggctt catgcttaga
      181 tgctttcagc acttatcccg tccacacata gctacccagc tatgccgttg gcacgacaac
      241 tggtacacca gaggtatgtc catcccggtc ctctcgtact aaggacagct cctctcaaat
      301 ttcctacgcc cacgacggat agggaccgaa ctgtctcacg acgttctgaa cccagctcgc
      361 gtaccgcttt aatgggcgaa cagcccaacc cttgggaccg actacagccc caggatgcga
      421 tgagccgaca tcgaggtgcc aaacctcccc gtcgatgtga actcttgggg gagataagcc
      481 tgttatcccc ggggtagctt ttatccgttg agcgatggcc cttccatgcg gaaccaccgg
      541 atcactaagt ccgtctttcg accctgctcg acttgtaggt ctcgcagtca agctccctta
      601 tgcctttaca ctctatgaat gatttccaac cattctgagg gaacctttga gcgcctccgt
      661 taccttttag gaggcgac
//
LOCUS       DAOBBH010000369          677 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.374, whole genome shotgun sequence.
ACCESSION   DAOBBH010000369 DAOBBH010000000
VERSION     DAOBBH010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.374"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..300
                     /gene="lpl1"
                     /locus_tag="P6L01_002734"
     CDS             <1..300
                     /gene="lpl1"
                     /locus_tag="P6L01_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl1"
                     /protein_id="HDP3031730.1"
                     /translation="GQYANLKELRKYNNGDVSINENVPSYDVEYKMSNKDEIVKELRS
                     RYNISTEKSPILKMHIDGDLKGSSVGYRKLEIDFSKRENSKLSVIEFLSYKPAKK"
     gene            336..>677
                     /locus_tag="P6L01_002735"
     CDS             336..>677
                     /locus_tag="P6L01_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDP3031731.1"
                     /translation="MSKKLALFISVIILGIFIIGCDSSSDTAEKAKEDSKEEQIKKSF
                     AKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWTLLTSFSKSNKPGEIDDEGMVLYL
                     NRNTKKATGYYF"
BASE COUNT          279 a           69 c          135 g          194 t
ORIGIN      
        1 ggtcaatatg caaatcttaa agaattgaga aaatataata atggtgatgt ttcaattaat
       61 gaaaatgttc ctagttatga tgttgaatac aaaatgagca ataaagatga aatagttaag
      121 gaattaagaa gtcgttataa tatttcgact gaaaaatcac ctatattaaa aatgcatatt
      181 gatggggacc tgaaaggcag ttctgtaggt tatagaaagt tagaaattga cttttcaaaa
      241 cgtgaaaaca gcaaattatc agtcattgaa tttttaagtt ataaaccagc gaaaaaatag
      301 tattgataag gaatattagg atgtgaaaaa atgggatatc taaaaagctt gcactgttca
      361 taagtgttat tattttgggc atttttataa taggttgtga tagttcaagc gatactgcgg
      421 aaaaagcaaa agaagattca aaagaagaac aaatcaaaaa gagctttgcg aaaacgttag
      481 atatgtatcc aattaagaat ctcgaggatt tatacgacaa agaaggatat agagatggtg
      541 aatttaaaaa gggcgataag gggacttgga ctttactcac aagtttttca aaaagtaaca
      601 aaccgggtga aatagatgac gaaggcatgg ttttatatct aaatagaaat accaaaaagg
      661 caacaggtta ttatttt
//
LOCUS       DAOBBH010000370          666 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.375, whole genome shotgun sequence.
ACCESSION   DAOBBH010000370 DAOBBH010000000
VERSION     DAOBBH010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.375"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            141..560
                     /locus_tag="P6L01_002736"
     CDS             141..560
                     /locus_tag="P6L01_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HDP3031732.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
BASE COUNT          257 a           89 c           97 g          223 t
ORIGIN      
        1 gtataccttt tgcctttaac ttaagttatt agagcctctt atgcagttgc tcagatcatc
       61 gtataaaaat taatgacgtc atttcaaaaa tcgatacaaa aataatttat tataaaaatt
      121 ctaagaaaga agtgaagcag atgttaaaat ctattaatca tatatgcttt tcagtcagaa
      181 atttaaacga ttcaatacat ttttatagag atattttact tgggaaattg ctattgactg
      241 gtaaaaaaac tgcttatttt gagcttgcag gcctatggat tgctttaaat gaagaaaaag
      301 atataccacg taatgaaatt cacttttcat atacacatat agctttcact atagatgaca
      361 gcgaatttaa atattggcat cagaggttaa aagataataa cgtgaatatt ttagaaggaa
      421 gagttagaga tattagagat agacaatcaa tttactttac tgaccctgat ggtcataagc
      481 tagaattaca tactggcaca cttgagaaca gattaaatta ttataaagag gctaaaccac
      541 atatgacatt ttacaaataa ggcgtcatta taaaaaggcc tcttgaaatc cgttaaaatt
      601 ttaattaatt attatataat aagagaactt ttcaaacaat acagttgtta tttttgctat
      661 ttcaac
//
LOCUS       DAOBBH010000371          657 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.376, whole genome shotgun sequence.
ACCESSION   DAOBBH010000371 DAOBBH010000000
VERSION     DAOBBH010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.376"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(58..>657)
                     /gene="sdpA"
                     /locus_tag="P6L01_002737"
     CDS             complement(58..>657)
                     /gene="sdpA"
                     /locus_tag="P6L01_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpA"
                     /protein_id="HDP3031733.1"
                     /translation="SVTRGNYFLTLMAAFFITIILFIGIVVSEVHLLYSMLIVLVGLI
                     LLRLFKINLFSFKKLTLSQVIYIIGGALLIYGLDNLYLYFHDVPANELQLEQEIRNTP
                     FYISIFTVTIIPAIVEEIVFRGMIIRVIFRKHLFLGLLVSSLVFASLHESDTWIGYLP
                     YLYSGLIFGLIYIKTKRLEVVIFMHFLNNLLALLFIIWG"
BASE COUNT          276 a           95 c           88 g          198 t
ORIGIN      
        1 caataaaata aatatcagat agataatttg aaaataatat gaaaggttat ctcaaaatta
       61 tccccatatt ataaagagca gagctaacaa gttatttaag aagtgcatga atattaccac
      121 ttctaatcgt tttgttttta tataaattag accaaaaatc aaaccagaat ataagtaagg
      181 taaataacca atccaagtgt cagattcgtg taatgatgca aaaactaaac tagacacaag
      241 taaccctaaa aataagtgtt ttctgaatat aacccttatt atcataccgc gaaaaacaat
      301 ttcttccaca atagcgggga tgatggtaac agtgaaaata gaaatataga atggtgtatt
      361 tcgtatttct tgctctaatt gtagttcatt tgccggtacg tcatgaaaat ataaataaag
      421 attatctaac ccataaatta atagtgcacc gcctataata taaataactt gagacaatgt
      481 taatttttta aaagagaata aattgatttt gaatagcctc aatagaatta aacctactaa
      541 tacaattagc atgctataaa gtaaatgtac ttcactgaca actatgccga taaataaaat
      601 gatagtaata aaaaatgctg ccattaaagt taaaaaataa tttcctcgtg taaccga
//
LOCUS       DAOBBH010000372          656 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.377, whole genome shotgun sequence.
ACCESSION   DAOBBH010000372 DAOBBH010000000
VERSION     DAOBBH010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.377"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(292..>656)
                     /locus_tag="P6L01_002738"
     CDS             complement(292..>656)
                     /locus_tag="P6L01_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDP3031734.1"
                     /translation="ASVIYLKVKYDEKEKQKAIYYKEQQERITLYLQHNTKEPNTIKS
                     VHFTSLKRGPMGDAVIEGYINENKKDNFTAYATPEHNYQFGGAMIESERLSELLKPAH
                     QLKSPDEIKEELDKKEGH"
BASE COUNT          187 a           98 c          112 g          259 t
ORIGIN      
        1 attgaaagga tattatacct tgctctaccc ttttttataa aaaatcacac acatttgaga
       61 aaatttgata atgtcaataa atcacgtcat cgccattgtt attcggtata taatgttttt
      121 ataatggcta aactattatt tcatttacat taatggggtt cgcttgtgtt aatgtactgt
      181 acaaaactgg ggaagtattg gtgatgatga agtgattgat attaattgcg aaacaccttc
      241 gatagcaatg acatagatta ttggaagatt aaatcaaaaa taaagaagac cctagtggcc
      301 ttcttttttg tctaattctt ctttgatttc gtcaggtgat tttaattgat gcgctggttt
      361 tagtaactca cttaatcttt cactttctat catagcacca ccaaactgat aattatgttc
      421 tggtgtagca taagcagtaa aattatcttt tttgttttca ttgatgtagc cttcaattac
      481 ggcatcgccc atgggtcctc tttttaaact tgtgaaatgc acagatttga tggtattggg
      541 ttctttggta ttatgctgga gatagagtgt aatacgctcc tgttgttctt tatagtatat
      601 tgctttttgt ttttcttttt cgtcatattt cacttttaaa taaatgactg atgcaa
//
LOCUS       DAOBBH010000373          655 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.378, whole genome shotgun sequence.
ACCESSION   DAOBBH010000373 DAOBBH010000000
VERSION     DAOBBH010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.378"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..571)
                     /gene="pgsA"
                     /locus_tag="P6L01_002739"
     CDS             complement(<1..571)
                     /gene="pgsA"
                     /locus_tag="P6L01_002739"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDP3031735.1"
                     /translation="MNIPNQITVFRVVLIPVFILFALVDFGFGNVSFLGGYEIRIELL
                     ISGFIFILASLSDFVDGYLARKWNLVTNMGKFLDPLADKLLVASALIVLVQLGLTNSV
                     VAIIIIAREFAVTGLRLLQIEQGFVSAAGQLGKIKTAVTMVAITWLLLGDPLATLIGL
                     SLGQILLYIGVIFTILSGIEYFYKGRDVFK"
BASE COUNT          259 a          129 c           66 g          201 t
ORIGIN      
        1 gtttaaaaac atctctacct ttataaaagt attcaatacc agataagata gtaaaaataa
       61 cgccaatgta taataaaatt tgtcctaatg acaaaccaat caatgttgcc aatggatcac
      121 ctaataacaa ccaagtaatt gctaccatag taactgctgt tttaatttta cctaattgac
      181 cagctgcact tacaaatcct tgttcaattt gtagtaaacg taaaccagtt acggcaaatt
      241 ctctggcaat aataatgatt gctactacag aatttgttag tcctagttgc acaagtacaa
      301 ttaaagcact tgcaactaat aatttatccg ctaatggatc caaaaatttc cccatatttg
      361 taactaaatt ccattttcta gctaaataac catcaacaaa atcgctaagg gaagccaata
      421 taaaaataaa accactgatt aataactcaa ttcttatttc atatcctcct agaaatgaca
      481 cattgccaaa tccaaaatca actaacgcaa acaatataaa aactggtatt aacactactc
      541 taaaaaccgt aatctggttc ggaatattca ttatacatcc tcatttctca ctaatttatt
      601 tctgttaaaa tattaaaact aacatgatcc ataacaataa agttattaaa actgt
//
LOCUS       DAOBBH010000374          637 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.379, whole genome shotgun sequence.
ACCESSION   DAOBBH010000374 DAOBBH010000000
VERSION     DAOBBH010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.379"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            1..562
                     /locus_tag="P6L01_002740"
                     /pseudo
     CDS             1..562
                     /locus_tag="P6L01_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          294 a           56 c           85 g          202 t
ORIGIN      
        1 tataaaaaat tttagaaata aattcggaaa atttctaagt caaagttttt tgaatagtaa
       61 taatgtaata aagcaagaga ttataaaaat tattaaagat gaatattatt attatgatca
      121 agaaaatacg aagaaatatt taataaagtt ttgtcaagat cttatagaac atataaaaga
      181 gcacagaaat caaatcaatg attgtcactt tacaaatata ttaaataaag atgcagaaaa
      241 aactaatagt tcaagtatct taacttcatt atttttaact gcaaacaata taccaaataa
      301 tctatcatct aatttttcta aagcagtgga atattatttt gcaaaaaaca aggaaatata
      361 tggagatgga tatgaaaagt atttagacga gtattttaga aatgttttag gcgaaaaaag
      421 ttatttaatt ttgatagatg atttttcagg gacaggaaaa tcaatcagcg actttattga
      481 tgcaataaaa aagtatatac taacattaaa aatagagatt attatatttt gcatacatat
      541 aaccgaagat gcagaaaaaa aaataatgga tacttcaata aaaaacggta ttgagataaa
      601 aatagctttt gtacaaaaaa cagagaaata ttttata
//
LOCUS       DAOBBH010000375          587 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.380, whole genome shotgun sequence.
ACCESSION   DAOBBH010000375 DAOBBH010000000
VERSION     DAOBBH010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.380"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..200
                     /gene="splD"
                     /locus_tag="P6L01_002741"
     CDS             <1..200
                     /gene="splD"
                     /locus_tag="P6L01_002741"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease SplD"
                     /protein_id="HDP3031736.1"
                     /translation="SVNGNIVTSDAVVQPGSSGSPILNSKREAIGVMYASDKPTGEST
                     RSFAVYFSPEIKKFIADNLDK"
     gene            complement(149..256)
                     /locus_tag="P6L01_002742"
     CDS             complement(149..256)
                     /locus_tag="P6L01_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031737.1"
                     /translation="MLILLLISKNHLSMYGWMIYLSKLSAINFLISGEK"
     gene            358..>587
                     /gene="splF"
                     /locus_tag="P6L01_002743"
     CDS             358..>587
                     /gene="splF"
                     /locus_tag="P6L01_002743"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplF"
                     /protein_id="HDP3031738.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTVVDGIQQTAKAENTVKQIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVT"
BASE COUNT          227 a           91 c           95 g          174 t
ORIGIN      
        1 tatcagtgaa tggaaatata gtgacatctg atgcggttgt ccaacctggc agctctggtt
       61 cacctatatt aaatagtaag cgagaagcaa ttggtgttat gtatgctagt gataaaccaa
      121 caggtgaaag tacaaggtca tttgctgttt atttctctcc tgaaattaag aaatttattg
      181 cagataattt agataaataa atcatccatc catacattga taaatgattt ttagaaatta
      241 acaacaaaat caacaatttt aaacatctct gtgattctat ttattcgaaa tgatttaaaa
      301 aataaaactt caaaaaccta accttatatt tatacgaata cttagaggag cacaaaaatg
      361 aataaaaata taatcatcaa aagtattgca gcattgacga ttttaacatc aataactggt
      421 gtcggcacaa cagtggttga tggtattcaa caaacagcca aagccgaaaa tactgttaaa
      481 caaattacaa atacaaatgt tgcaccatac agtggtgtta catggatggg cgctggaaca
      541 ggatttgtag ttggaaatca tacaatcatt accaataaac atgttac
//
LOCUS       DAOBBH010000376          586 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.381, whole genome shotgun sequence.
ACCESSION   DAOBBH010000376 DAOBBH010000000
VERSION     DAOBBH010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.381"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            384..557
                     /locus_tag="P6L01_002744"
     CDS             384..557
                     /locus_tag="P6L01_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031739.1"
                     /translation="MWNFIKCVFKFVFSLVAITTLVAGVGVVAFAYIFKKDFEDIERK
                     TKEIISDIESKNN"
BASE COUNT          216 a           54 c           98 g          218 t
ORIGIN      
        1 gtcatgttaa ataaagttat attttactaa atgaaatata aaattgtttg aggtgatttc
       61 tcggtgtata agacttatca atcagttaaa acatattttt atagatggtg gggatattaa
      121 gttaaaaact taaaatcatc ttatcataaa taacaatctt aagttagcat tcacgataat
      181 agtcattgtt aacattagca tataaggtca tgtcacgttg aaacagaggt tcctcggcat
      241 ttttgaaaat aatcattagc ttgctcacta tataatttga tgaatatatt tcgtaaaagt
      301 gggtatttat ttaatgatta ttctatatat gatagtataa tgaaatgtag ataggtattt
      361 aatttaacag aggtgaaatt gagatgtgga attttattaa atgtgtgttt aaattcgtat
      421 ttagcttagt tgctattaca acattagttg ctggtgttgg tgtagtagca tttgcttata
      481 tctttaaaaa agattttgaa gatattgaaa gaaaaactaa agaaattatt tctgatattg
      541 aaagtaaaaa taactaataa catttagagg ctgggacata aatccc
//
LOCUS       DAOBBH010000377          577 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.382, whole genome shotgun sequence.
ACCESSION   DAOBBH010000377 DAOBBH010000000
VERSION     DAOBBH010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.382"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(180..497)
                     /locus_tag="P6L01_002745"
     CDS             complement(180..497)
                     /locus_tag="P6L01_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HDP3031740.1"
                     /translation="MNKLLQSLSALGVSATLVTPNLNADATTNTTPQIKGANDIVIKK
                     GQDYNLLNGISAFDKEDGDLTDKIKVDGQIDTSKSGKYQIKYHVTDSDGAIKISTRYI
                     EVK"
BASE COUNT          189 a           82 c           91 g          215 t
ORIGIN      
        1 aaatcaaaac attttttaaa aagagcagtt aagttttaat aatttgatag gtagtccttt
       61 ggtatacaga aaaaatacaa tatttaggtt aaagttgcag atgaagttgg aataacaaat
      121 aaaatggtag caaaacatgt tcgtttgcta ccattttatt tacagtatag tgatgagggc
      181 tatttaacct caatatacct agtggaaatt ttaattgcac catctgaatc agtgacatga
      241 tatttaattt gatatttacc agatttagat gtatcaattt ggccatcgac tttaatttta
      301 tcggttaaat ctccatcttc tttatcaaat gcacttatgc cgtttagaag gttataatct
      361 tgacctttct taataacgat atcattagcg cctttaattt gtggtgtagt attcgtcgtt
      421 gcatctgcat ttaaatttgg tgttactagt gtagcagaaa caccgagggc tgataatgac
      481 tgtaatagtt tattcataat gtaacctccg aatgataagt catttctgag ttagctttac
      541 tataccttaa tttatattat ttacacatca tactata
//
LOCUS       DAOBBH010000378          575 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.383, whole genome shotgun sequence.
ACCESSION   DAOBBH010000378 DAOBBH010000000
VERSION     DAOBBH010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.383"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
BASE COUNT          205 a           95 c           88 g          187 t
ORIGIN      
        1 actgagtttt aatgaactct tttaacccac attaaatatt attttgcaat tgcaatgaat
       61 aacaagaaaa acctgggaca ttaatcgatc aaatgctccc ttcaaagtag acattgaata
      121 aatgaaggct ttgaagggag catttcactt tgttcttggc tcaacaattt tatatagaca
      181 gtagttaatt gaatgaaaat aagcttgtaa caagcatttt tcaattatag tccggggccc
      241 caacaaagaa gctgacgaaa agtcagctta caataatgtg caagttgggg atgggcccca
      301 acacagagaa tttcgaaaag aaattctaca gacaatgcaa gtcagcgagg ccccaacaaa
      361 gaagctgtga ctatgataaa gtactactac atagttaatc attagtggtt ctttatcatt
      421 ttcgcctccc ttttcttatt gttttgatac acaaaaattt aagttcaaac tgtcgaataa
      481 agttatattt gatttcaaat tatccctaaa ttattaattt tacaattgtg gcagattttc
      541 aaaataataa ttatttcctc attatttata aattt
//
LOCUS       DAOBBH010000379          575 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.384, whole genome shotgun sequence.
ACCESSION   DAOBBH010000379 DAOBBH010000000
VERSION     DAOBBH010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.384"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..393)
                     /locus_tag="P6L01_002746"
     CDS             complement(<1..393)
                     /locus_tag="P6L01_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDP3031741.1"
                     /translation="MAKINFDAATKGNPGISTCAIVIKEDKQHYTYTHELGEMDNHTA
                     EWAACIYALEHARELNVQNALLYTDSKLIADSIEAGYVKNANFKPYFDQIEIFEKDFD
                     LLFVKWIPREQNKEANQHAQQALYKLIKK"
BASE COUNT          176 a          102 c           76 g          221 t
ORIGIN      
        1 ctttttaatt aatttataca atgcctgttg agcgtgttga ttcgcttctt tgttttgttc
       61 tctcggtatc catttaacaa ataataaatc aaaatctttt tcaaatattt ctatttgatc
      121 aaaataaggt ttgaaatttg cgtttttcac ataaccagct tcaatgctat ctgcaattag
      181 ctttgagtct gtatataata gtgcgttttg aacatttaat tcacgtgcat gttctagtgc
      241 ataaatacat gcagcccatt ctgcagtgtg gttatccatt tcgcctaact catgtgtata
      301 tgtataatgc tgcttatctt ctttgattac aatggcacat gtacttatgc ctggatttcc
      361 tttcgtcgca gcatcaaaat ttattttcgc cataataaac ctactttcta ttcaatactt
      421 agttaaagtt actattactg taatacaaaa tatgttgggt aatccattaa aaaacacgca
      481 tcacttaaat aagtaacacg tgtttaaaat actcgctgat tcaaagatga ttttctaata
      541 cgtagtactg taatatactt cctaaaaaaa tcatc
//
LOCUS       DAOBBH010000380          568 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.385, whole genome shotgun sequence.
ACCESSION   DAOBBH010000380 DAOBBH010000000
VERSION     DAOBBH010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.385"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..210
                     /locus_tag="P6L01_002747"
     CDS             <1..210
                     /locus_tag="P6L01_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HDP3031742.1"
                     /translation="YNVKQLRKRYNIPTNKAPKLLIKGDGDLKGSSVGSKNLEFTFVE
                     NKEENIYFTDSVQYTSSEDTSYESN"
     gene            complement(214..336)
                     /locus_tag="P6L01_002748"
     CDS             complement(214..336)
                     /locus_tag="P6L01_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031743.1"
                     /translation="MSYIIKFTFKFFEMATYISQLTLDIELVSSELLVILPGFI"
BASE COUNT          235 a           79 c           96 g          158 t
ORIGIN      
        1 tacaatgtga agcaacttcg aaaaagatat aatattccaa ccaacaaagc tccgaaatta
       61 ttaataaaag gtgatggtga tttaaaaggc tcatctgtag ggtcaaaaaa tctagaattt
      121 acctttgtag aaaataaaga agaaaacatt tattttacag attctgtaca atatacttca
      181 agtgaggata caagttatga gtcaaactga atatcaaata aaacctggta atataacaag
      241 taactctgaa gaaacaagtt cgatatctaa agtgagctgt gaaatatagg tagccatttc
      301 aaaaaattta aaggtgaatt tgataatgta gctcaaggag attgggttaa aaaggcgaag
      361 aatgaactgg atgatattag taagaaatta aaaaatattc aaagaacgga agtttaatat
      421 tctagccgat tcctgaaact aagacagcat gcgttcattc atgccattat taatataagc
      481 accgcaacaa aaaagcttct aatgtgatac aggaacctca tattccgtat catgttagaa
      541 gcttttaatg tctaaagaac atctacat
//
LOCUS       DAOBBH010000381          554 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.386, whole genome shotgun sequence.
ACCESSION   DAOBBH010000381 DAOBBH010000000
VERSION     DAOBBH010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.386"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            106..>554
                     /locus_tag="P6L01_002749"
     CDS             106..>554
                     /locus_tag="P6L01_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDP3031744.1"
                     /translation="MRDSKRVVLYISIMVLSIFIIGCGKGNEIKEDAKEEQIKKSFAK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGMWTIYTDFAKSNRPGVLDNEGMVLNLDR
                     NTRTAKGYYFVDTIYDNHENSYSKNYRVEMKNNKIILLDKVEDQKLK"
BASE COUNT          240 a           54 c          111 g          149 t
ORIGIN      
        1 aatatatatt tttcagatgg attaaactta attccaagtg actaaaaata acacaaaggt
       61 tgatagtact caacaaatat taacatgata gagagaagtg aaaaaatgag ggattcaaaa
      121 agagtagtat tgtacataag cattatggtg ttgagtattt ttataattgg ttgtggcaaa
      181 ggtaatgaaa taaaagaaga cgcaaaggaa gaacaaatca aaaagagctt tgcgaaaacg
      241 ttagatatgt atccaatcaa aaatctcgag gacctatatg ataaagaagg atatcgagat
      301 ggcgaattta aaaaaggtga caaagggatg tggacgattt acacagattt cgccaaaagt
      361 aatagaccag gtgtattaga taatgaaggt atggttttaa atttggatag aaatacacga
      421 acggccaagg gatattattt tgtagatact atatatgaca atcatgaaaa ctcttatagt
      481 aaaaattata gagttgagat gaaaaacaat aaaattattt tattagacaa ggtggaagat
      541 caaaaactta aaga
//
LOCUS       DAOBBH010000382          542 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.387, whole genome shotgun sequence.
ACCESSION   DAOBBH010000382 DAOBBH010000000
VERSION     DAOBBH010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.387"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            85..>542
                     /locus_tag="P6L01_002750"
     CDS             85..>542
                     /locus_tag="P6L01_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDP3031745.1"
                     /translation="MIHSKKLTLGICLVLLIILIVGYVIMTKTNGRNAQIKDTFNQTL
                     KLYPTKNLDDFYDKEGFRDQEFKKGDKGTWIVNSEMVIEPKGKDMETRGMVLYINRNT
                     RTTKGYYFISEMTDDSNGRPKDDEKRYPVKMEHNKIIPTKPLPNDKLRKE"
BASE COUNT          228 a           69 c          100 g          145 t
ORIGIN      
        1 tcataaagtg ttcaaaataa tttttcgctt tagttgtata aattaaagat tgagtcaata
       61 ttaaaaatat aaggtggaga aatgatgatt cattcaaaga aattaacgct cggtatatgc
      121 ttggtattac tcattatatt gattgtaggt tatgtcatta tgacaaaaac aaatggtcga
      181 aacgcccaaa ttaaagacac atttaatcaa acattaaaat tatatccaac caaaaatctc
      241 gatgattttt acgataaaga aggctttcga gatcaagaat ttaaaaaggg tgataaaggt
      301 acttggatag ttaattctga aatggtaatc gagccaaaag gtaaggatat ggaaacgaga
      361 ggaatggtgc tctatatcaa tcgcaatact agaaccacaa aagggtatta ttttataagt
      421 gaaatgacag atgacagtaa cggcagacca aaggatgatg aaaaaaggta tccggtaaaa
      481 atggaacata ataaaatcat accaacgaag ccactaccga atgacaagtt aagaaaagaa
      541 at
//
LOCUS       DAOBBH010000383          538 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.388, whole genome shotgun sequence.
ACCESSION   DAOBBH010000383 DAOBBH010000000
VERSION     DAOBBH010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.388"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            8..517
                     /locus_tag="P6L01_002751"
     CDS             8..517
                     /locus_tag="P6L01_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDP3031746.1"
                     /translation="MTVDNNKAKQAYDNQTGVNEKEREERQKQQEQNQEPQFKNKLTF
                     SDEVVEKIAGIAAREVKGILDMKGGLTDTFTNAFSSGNNVTQGVSVEVGEKQAAVDLK
                     VILEYGESAPKIFRKVTELVKEQVKYITGLDVVEVNMQVDDVMTQKEWKQKHEKNNEN
                     NNQERQGLQ"
BASE COUNT          232 a           71 c           98 g          137 t
ORIGIN      
        1 atctattatg actgtagata acaataaagc aaaacaagca tacgacaatc aaactggtgt
       61 taacgaaaaa gaaagagaag agcgtcaaaa acaacaagaa caaaatcaag agcctcaatt
      121 caaaaataaa ttaactttct ctgatgaagt tgttgaaaaa attgctggta tcgctgcacg
      181 tgaagttaaa ggtatcttag acatgaaagg tggcttaact gatacattca ctaatgcatt
      241 ctcaagtgga aataacgtta ctcaaggtgt atctgttgaa gttggtgaaa aacaagctgc
      301 tgtagactta aaagtaattt tagaatatgg tgaatcagca ccaaaaatct tccgtaaagt
      361 aactgaatta gttaaagaac aagttaaata tattactggt ttagatgttg ttgaagttaa
      421 catgcaagtt gatgatgtaa tgactcaaaa agaatggaaa caaaaacacg aaaaaaataa
      481 tgaaaacaat aaccaagaaa gacaaggttt acaataagtt attcgataaa aagggctt
//
LOCUS       DAOBBH010000384          530 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.390, whole genome shotgun sequence.
ACCESSION   DAOBBH010000384 DAOBBH010000000
VERSION     DAOBBH010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.390"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..426
                     /locus_tag="P6L01_002752"
     CDS             <1..426
                     /locus_tag="P6L01_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDP3031747.1"
                     /translation="ISKNQNEKKYRVELKNNKIILLDNVEDEKLKQKIENFKFFSQYA
                     DFKDLKNYQDGSITTNENVPRYEAEYKLNNSDTNVKKLRDIYPITTKKAPILKLHIDG
                     DIKGSSVGYKKIEYKFSKVKDQETTLRDYLNFGPSDEDS"
BASE COUNT          239 a           47 c           89 g          155 t
ORIGIN      
        1 attagcaaaa atcagaatga gaaaaaatac cgtgttgaac ttaaaaataa taagattatt
       61 cttttggata atgtagaaga cgaaaaactt aaacaaaaaa ttgaaaattt taaatttttt
      121 agtcagtatg ccgattttaa agatttaaaa aattatcaag atggaagtat aacaactaat
      181 gaaaatgtac cgagatatga agcagaatac aaattgaata atagtgatac aaatgtaaaa
      241 aaacttagag atatttatcc aattacaacg aaaaaggctc caatattaaa gttacatata
      301 gatggtgata taaaaggaag ttcagttgga tacaaaaaaa tagaatataa attttcaaaa
      361 gttaaagatc aagagacaac attaagagat tatttaaatt ttgggccgtc tgatgaagat
      421 agctaaagtt tattagatgt agaaataatt ctaaagcaga gagacgtgaa atgatgggat
      481 attcaaaaag gtttgcattg tacataagcg ttatgatttt aatatttgcg
//
LOCUS       DAOBBH010000385          505 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.391, whole genome shotgun sequence.
ACCESSION   DAOBBH010000385 DAOBBH010000000
VERSION     DAOBBH010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.391"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..349
                     /locus_tag="P6L01_002753"
     CDS             <1..349
                     /locus_tag="P6L01_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HDP3031748.1"
                     /translation="KKEIENFKFFVQYGNFKDFKDYKNGDISYNPNVPSYSAKYHLSN
                     DDYNIQQLRKRYDIPTKKAPELLLKGDGDLKGSSIGSKDLEFTFVQNKRENIYFTDSV
                     EFTPSEDTSYESN"
BASE COUNT          212 a           54 c           83 g          156 t
ORIGIN      
        1 aaaaaaagaa attgaaaact tcaagttttt tgtacaatat ggcaacttta aagattttaa
       61 agattataaa aatggggata tttcatacaa tccaaacgta ccaagttatt cggcaaaata
      121 tcatttaagt aatgatgatt ataatatcca acaattaaga aaaagatatg atataccgac
      181 taaaaaagca ccagaactat tgttgaaagg tgatggcgat ttaaaaggtt catccatagg
      241 ctctaaagac ttagaattta cctttgtaca aaataaaaga gagaatatct attttacaga
      301 ttcggttgaa tttactccaa gcgaggatac aagttatgag tcaaactaaa tagggttaat
      361 agaagtttgg attgaaaatt tcataaagtg ttaaaaaatt tttcgcttta aatgtataaa
      421 ttaaagattg agtcagtatt aaaaatataa ggtggagaaa tgatgattca ttcaaaaagg
      481 ttaaaattgt gtctatgcct gataa
//
LOCUS       DAOBBH010000386          471 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.393, whole genome shotgun sequence.
ACCESSION   DAOBBH010000386 DAOBBH010000000
VERSION     DAOBBH010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.393"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
BASE COUNT          165 a           82 c           62 g          162 t
ORIGIN      
        1 catagaagct ggcggaaagt cagcttacaa taatgtgcaa gttgggtggg acgacgataa
       61 agaaatactt tttctataga aattagtatt tcttatacat gagttttact catgtattcc
      121 tatttttaag tacacattag ctgtggctaa tgtgtaagaa ccactacata ataaatcatt
      181 agtggctctt tatcatttct gtcccactcc cttgagccta caattttgat tatgtatcac
      241 gttcataata ctttccaatt tgaaataccc tgttcgcaat tattagttga gttatatcaa
      301 ccaatacagt gtaaacataa aaattccaaa ccgtttaaca gtatatcaga aaaaatgata
      361 atcatttctt ttatttttat taaatttcat aatgattcat ataatattcg cattaaaaca
      421 tcacaatact gatacacaac aaaaagagca gtacatttgt catgcaccac t
//
LOCUS       DAOBBH010000387          466 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.394, whole genome shotgun sequence.
ACCESSION   DAOBBH010000387 DAOBBH010000000
VERSION     DAOBBH010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.394"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..439
                     /gene="sei"
                     /locus_tag="P6L01_002754"
     CDS             <1..439
                     /gene="sei"
                     /locus_tag="P6L01_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031927819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="staphylococcal enterotoxin type I"
                     /protein_id="HDP3031749.1"
                     /translation="KSKYMYGGATLSGQYLNSARKIPINLWVNGKHKTISTDKIATNK
                     KLVTAQEIDVKLRRYLQEEYNIYGHNNTGKGKEYGYKSKFYSGFNNGKVLFHLNNEKS
                     FSYDLFYTGDGLPVSFLKIYEDNKIIESEKFHLDVEISYVDSN"
BASE COUNT          195 a           51 c           67 g          153 t
ORIGIN      
        1 taaatctaaa tacatgtatg gaggggccac tttatcagga caatacttaa attctgctag
       61 aaaaatccct attaatcttt gggttaatgg caaacataaa acaatttcta ctgacaaaat
      121 agcaactaat aaaaaactag taacagctca agaaattgat gttaaattaa gaagatatct
      181 tcaagaagaa tacaatatat atggtcataa taacactggt aaaggcaaag aatatggata
      241 taaatctaaa ttttattcag gttttaataa tgggaaagtt ttatttcatt taaataatga
      301 aaaatcattt tcatatgatt tgttttatac aggagatgga ctgcctgtaa gttttttgaa
      361 aatttatgaa gataataaaa taatagaatc tgaaaaattt catcttgatg tcgaaatatc
      421 atatgtagat agtaactaat gataattagt tttaacacta aaatgc
//
LOCUS       DAOBBH010000388          462 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.395, whole genome shotgun sequence.
ACCESSION   DAOBBH010000388 DAOBBH010000000
VERSION     DAOBBH010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.395"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     regulatory      complement(35..135)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT          169 a           64 c           85 g          144 t
ORIGIN      
        1 aaagttatgc gttcacaata tatattagta aaacatatgt atagtaacac tttcctacgc
       61 tagttcaagc tagatcaggt tcaaagggtt tgagggcaag cctcatctca gtataaaaca
      121 cccctagtgt gtgcgattta tttaattaat tatactgtaa gacgtttgta aacttatgtc
      181 aataggttgt cttcatgaaa tttcgtttaa ttcgatttaa aatttataat attagcattg
      241 gatttaaatt gaagatgtag taggaatgtt agtaattaaa gatataaaaa tatgtgacat
      301 gtaataagat tgagctgata aatgaagagg gatacttatc aatcatacct ctttaacaac
      361 agtgaagaac ccgtgcataa tggcttacga attatagttt gtagggtaaa agagggatac
      421 atgcgccgag cacatgcaaa aaagccccta acaactaaaa gt
//
LOCUS       DAOBBH010000389          453 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.396, whole genome shotgun sequence.
ACCESSION   DAOBBH010000389 DAOBBH010000000
VERSION     DAOBBH010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.396"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
BASE COUNT          123 a           84 c           74 g          172 t
ORIGIN      
        1 catttttgta tgctgacttt tcgccagctt ctgtgttggg ccccacccca acttgcattg
       61 tctgttgaaa ttggtgatcc aatttctcta tgttggggcc ccgccaactt gcattgtctg
      121 ttggaattgg tgatcctatt tctctctgtt gtggccccac cccaacttgc attgtttgta
      181 gaatttcttt tcgaaattct ctgtgttggg gccccacgag tattgaaaaa acttgttata
      241 agcttatttt catttagtca actactacca atatagcact agaactggaa atgataattt
      301 aataatgtac acttttgatt atttaaacat gtacatattt taaaaaatag aagagcaaag
      361 taaataattg atatagttat tttgagagta atcctaggaa ctattgtatt tatatttgtc
      421 tcccctactt ttaaatgtca ttcattatac ata
//
LOCUS       DAOBBH010000390          446 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.397, whole genome shotgun sequence.
ACCESSION   DAOBBH010000390 DAOBBH010000000
VERSION     DAOBBH010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.397"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..427
                     /locus_tag="P6L01_002755"
     CDS             <1..427
                     /locus_tag="P6L01_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDP3031750.1"
                     /translation="VEFFEWFNTLETFKNWKKKPGKPLDVQLDELADMLAFGLSIANQ
                     VGVSSEEIKEAIESSFKDTEFHKMFNFKDKEFAQDAVVSTPQIIFKEFYPDQQAIVIV
                     IDIAYNLYSIDQLIDAYKKKMKRNHERQDGTADAGKGYV"
BASE COUNT          176 a           65 c           91 g          114 t
ORIGIN      
        1 agttgaattc tttgaatggt ttaatacatt ggaaacgttt aagaactgga agaagaaacc
       61 aggtaagccg ttagacgtac aacttgatga attagctgac atgttggcgt ttggattgag
      121 tattgcgaat caagtaggag tgtcatcaga agagataaaa gaagcgattg aatcaagttt
      181 taaagataca gaatttcaca aaatgtttaa ttttaaagat aaagaatttg ctcaagacgc
      241 agttgttagt acaccacaga taatattcaa agaattttat cccgaccaac aagcaattgt
      301 tatagtgata gacatagctt acaacttata ttctatcgac caactcattg acgcatacaa
      361 aaagaaaatg aaaaggaacc acgaaagaca agatggaaca gcagacgcag gaaaaggata
      421 cgtgtaaaga catcttagat cgagtc
//
LOCUS       DAOBBH010000391          439 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.398, whole genome shotgun sequence.
ACCESSION   DAOBBH010000391 DAOBBH010000000
VERSION     DAOBBH010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.398"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..210
                     /locus_tag="P6L01_002756"
     CDS             <1..210
                     /locus_tag="P6L01_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDP3031751.1"
                     /translation="LEPWTSCEFDFTREGNLKVSFDYIDWIKLGFGPSGKENYYMYKK
                     FGVLPETEYEMEEIREVEKYVKDQE"
BASE COUNT          168 a           46 c           83 g          142 t
ORIGIN      
        1 ttagaaccat ggacatcatg tgaatttgat tttacaagag agggtaattt gaaagtatcg
       61 tttgattata tagattggat taaattaggt tttggtccat caggaaagga aaactactat
      121 atgtataaaa agtttggtgt tttaccagaa acggaatatg aaatggaaga aattcgagaa
      181 gtagagaagt atgttaaaga ccaagagtag catatatgtt atagaaggct gtgcaaaatc
      241 accctcgttt tacatttgac tcaaagaaga aggtaaacga taagattatt tgcaacttaa
      301 aaagtttatt agcttctcgg tatccataca tcatatgtat aaatgagttt aactaattaa
      361 taaatcacga tataaattta aaattgccat taattgaaaa ggttattgta gtgtatttta
      421 taaagttgat gcacatcga
//
LOCUS       DAOBBH010000392          433 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.399, whole genome shotgun sequence.
ACCESSION   DAOBBH010000392 DAOBBH010000000
VERSION     DAOBBH010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.399"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            126..>433
                     /locus_tag="P6L01_002757"
     CDS             126..>433
                     /locus_tag="P6L01_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HDP3031752.1"
                     /translation="MKKLCSLIVVALICIIALSACGKEQTKTYEGDVSGKHVLTSITY
                     KDDKVLKQSTINTIKYDDLGMDKDEAKKLFAKSESIFKDLKGVKYKVDYKDKKAIEHL
                     "
BASE COUNT          186 a           56 c           62 g          129 t
ORIGIN      
        1 ggaatccgct taaaaaactc atatcttcaa aaaaaatgac aaaatttaaa tttttgaata
       61 acatcatttt ttagtaaatg ttataatttc tttataatac aaatatcgaa agaaggcgac
      121 tgactttgaa aaaattatgt tcattaattg tagtagcatt aatttgtatc attgcattat
      181 cagcttgtgg taaagaacaa accaaaacat atgaaggtga tgttagtggt aaacatgtac
      241 taacttctat cacatataaa gatgataagg ttttaaaaca gtctacaatc aacacaatta
      301 aatatgacga tctaggtatg gataaagacg aagccaaaaa gttattcgcc aaatctgaaa
      361 gtattttcaa agaccttaaa ggcgtaaaat acaaagtaga ctataaagat aaaaaagcaa
      421 ttgaacactt aga
//
LOCUS       DAOBBH010000393          432 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.400, whole genome shotgun sequence.
ACCESSION   DAOBBH010000393 DAOBBH010000000
VERSION     DAOBBH010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.400"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            24..>432
                     /locus_tag="P6L01_002758"
     CDS             24..>432
                     /locus_tag="P6L01_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDP3031753.1"
                     /translation="MRYLKRLSWYISILILIVVIAGCGKGNETKEGSKEEQIKKSFAK
                     TLDMYPIKNLEDLYDKEGYRDSEFKKSDKGTWTIYTDFAKSNKPGELDDEGMVLNLDR
                     NTRTAKGHYFVTTFYRNGKLPDEKNYKIEMKNNK"
BASE COUNT          186 a           49 c           87 g          110 t
ORIGIN      
        1 ataaaacaaa gagacgtgaa acgatgcgat atttaaaaag actttcatgg tacataagca
       61 tcttaatttt aatagttgtt atagctggtt gtggcaaagg taatgaaaca aaagaaggtt
      121 caaaagaaga acaaatcaaa aagagctttg cgaaaacgtt agatatgtat ccaatcaaaa
      181 atctcgagga tttatacgac aaagaaggat atcgagatag cgaatttaaa aaaagtgata
      241 aagggacatg gacgatatac acagatttcg ctaaaagtaa taaaccgggt gaattggatg
      301 atgaaggtat ggttttaaat ctggatagga atactagaac cgctaaaggc cattattttg
      361 ttactacatt ttaccggaat ggtaaactac cagatgaaaa gaattataaa attgaaatga
      421 aaaataataa ga
//
LOCUS       DAOBBH010000394          425 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.401, whole genome shotgun sequence.
ACCESSION   DAOBBH010000394 DAOBBH010000000
VERSION     DAOBBH010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.401"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..>425
                     /locus_tag="P6L01_002759"
     CDS             <1..>425
                     /locus_tag="P6L01_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDP3031754.1"
                     /translation="EAKLKLLQDITNLLNYIENREKIETKIANSKKDISKTNNKILNL
                     DCDKRNIDKEKKMLEENGDLIESKTSFIDKTRVLFNDINKYQQSYLNIECLITEGEQL
                     GDELNNLIKGLEKVEDSIGNNESDYEKIIELNNAITNIN"
BASE COUNT          200 a           39 c           66 g          120 t
ORIGIN      
        1 gaagctaagt taaagttgtt acaagatatt accaacctat tgaattatat tgaaaataga
       61 gaaaaaattg aaactaaaat tgctaatagc aaaaaagata tttctaaaac caataataaa
      121 atattgaatc ttgattgtga taagcgaaac atagacaaag agaaaaaaat gttagaagaa
      181 aacggagatt taattgaaag taaaacctct tttattgata aaactagagt attatttaac
      241 gatattaata agtatcaaca aagttatctc aatattgaat gcttgataac tgagggtgaa
      301 caattaggtg atgaattaaa taatctaatt aaaggtttag aaaaggtcga agattcaata
      361 ggtaataacg aaagtgatta cgagaaaatt atcgaactaa ataatgcgat aactaacata
      421 aataa
//
LOCUS       DAOBBH010000395          424 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.402, whole genome shotgun sequence.
ACCESSION   DAOBBH010000395 DAOBBH010000000
VERSION     DAOBBH010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.402"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            81..257
                     /locus_tag="P6L01_002760"
     CDS             81..257
                     /locus_tag="P6L01_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HDP3031755.1"
                     /translation="MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIAL
                     IGLVIKLIELSNKK"
     gene            complement(310..417)
                     /locus_tag="P6L01_002761"
     CDS             complement(310..417)
                     /locus_tag="P6L01_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031756.1"
                     /translation="MIFILWCVNLYIEKGQHAQTCYPSEPVKKTVAILD"
BASE COUNT          142 a           60 c           82 g          140 t
ORIGIN      
        1 agttttgaac aaagcgagaa taatctgaaa gtatacaagc agtaaaaaaa gtatatgtgc
       61 tataatatgc tttgagcaag ttggatagat ggtggctatc tgagtataag gaggtggtgc
      121 ctatggtggc attactgaaa tctttagaaa ggagacgcct aatgattaca attagtacca
      181 tgttgcagtt tggtttattc cttattgcat tgataggtct agtaatcaag cttattgaat
      241 taagcaataa aaaataacca tcgctaactt tggctggttt cgatggttaa atggttatta
      301 atttaatctt taatctaaaa tagccaccgt ctttttaacg ggctcactag ggtaacatgt
      361 ttgcgcatgt tgcccttttt ctatatataa attaacacac cataatataa atatcaaata
      421 gacg
//
LOCUS       DAOBBH010000396          416 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.403, whole genome shotgun sequence.
ACCESSION   DAOBBH010000396 DAOBBH010000000
VERSION     DAOBBH010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.403"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..319
                     /locus_tag="P6L01_002762"
     CDS             <1..319
                     /locus_tag="P6L01_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031757.1"
                     /translation="FLAYKKVPKILVDKIIAIMYVKNHADMKSKFLEIEINRILLYND
                     NILQECLSQKFLIKQTDFYIITYAGIELLKKYGLEKITLSNILYEFKSNGLHQQKNFD
                     GNK"
BASE COUNT          177 a           36 c           58 g          145 t
ORIGIN      
        1 gtttctggca tataaaaaag ttcctaaaat tttggtagat aaaattatag caataatgta
       61 tgttaaaaat catgctgata tgaaatcgaa atttttagaa attgagatta ataggatatt
      121 attatataat gacaatatat tgcaagaatg tctttctcaa aaatttctaa ttaaacaaac
      181 agatttttat attattactt atgcaggaat agaattacta aaaaaatatg gattagaaaa
      241 aataacttta agtaatatac tttatgaatt taagagtaac ggattacatc agcagaaaaa
      301 ttttgatggt aataaataaa atgagcaaga gtgtctgata agcgagtcgt cttatctaag
      361 aattaaggta tacaattgat tacagtaaat ttttcttcga aaattttact ataaat
//
LOCUS       DAOBBH010000397          384 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.405, whole genome shotgun sequence.
ACCESSION   DAOBBH010000397 DAOBBH010000000
VERSION     DAOBBH010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.405"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..347
                     /locus_tag="P6L01_002763"
     CDS             <1..347
                     /locus_tag="P6L01_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDP3031758.1"
                     /translation="GEPTPPKPEVPSEPETPVPPTPEVPSEPGKPVPPAKEEPKKPSK
                     PVEQGKVVTPVIEINEKVKAVAPTKQKQSKKSELPETGGEESTNKGMLFGGLFSILGL
                     VLLRRNKKNNKA"
BASE COUNT          162 a           77 c           69 g           76 t
ORIGIN      
        1 caggcgaacc aacgccacca aaaccggaag taccaagtga gccggaaaca ccagtaccac
       61 caacaccaga ggtaccatct gaacctggta aaccagtacc acctgctaaa gaagaaccta
      121 aaaaaccttc taaaccagtg gaacaaggta aggtagtaac acctgttatt gaaatcaatg
      181 aaaaggttaa agcagtggca ccaactaaac aaaaacaatc taagaaatct gaactacctg
      241 aaacaggtgg agaagaatca acaaacaaag gtatgttgtt cggcggatta ttcagcattc
      301 taggtttagt attattacgc agaaataaaa agaataacaa agcataatta aacaaaaatt
      361 gacgggttta tttcataaat tata
//
LOCUS       DAOBBH010000398          382 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.406, whole genome shotgun sequence.
ACCESSION   DAOBBH010000398 DAOBBH010000000
VERSION     DAOBBH010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.406"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
BASE COUNT          139 a           66 c           97 g           80 t
ORIGIN      
        1 ggtagcaaac ttacgttccg ctagagtaga acgttgccag gcaagttgag accgcaaggt
       61 ctcttttttt atgtctaaaa cgtcaaaata aaagcaaaca caaagaaaga tggcttggcg
      121 aagtgaaaac gtttgaatct gacgaaacga gaaaagagtg caacgagttt agtagagcta
      181 aatgagtaag cgagagccga aggagaggaa agaagcaagc gattgtcaca agtcaagaaa
      241 ggttcttagc gacgaaggta gccaacttac gttccgctag agtagaacgt tgccaggcaa
      301 aaagtggatg cgatgagccg cattgagacc gcaaggtctc tttttttatg tctaaagtta
      361 catagtaaaa agcaaacaca aa
//
LOCUS       DAOBBH010000399          380 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.407, whole genome shotgun sequence.
ACCESSION   DAOBBH010000399 DAOBBH010000000
VERSION     DAOBBH010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.407"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            142..>380
                     /locus_tag="P6L01_002764"
     CDS             142..>380
                     /locus_tag="P6L01_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDP3031759.1"
                     /translation="MRAFTEANKKSKYQEQTNNARKSEASNCPICNTDLQYDHQTHIW
                     NYKDMVGDHIIPWSKGGKTERGNLQMLYKHHNSLKS"
BASE COUNT          160 a           46 c           60 g          114 t
ORIGIN      
        1 tatcataaca actcttaaaa tccttcagta ttagaatata aacttttaag tttatatgaa
       61 gatagtgatg tttcaaggaa gctaggaatt tttgagtatt tattaagcgg agaaaaatta
      121 gaatttataa aataactatc aattagagct ttcactgaag cgaataaaaa gtctaaatat
      181 caagagcaaa ctaataatgc tcgaaaaagt gaagcttcaa attgtcctat ttgtaataca
      241 gatttacaat atgatcacca aacacatata tggaattata aagatatggt aggagatcat
      301 attataccat ggagtaaagg tggaaaaaca gagcgtggaa acttacagat gctttataaa
      361 catcataata gtctaaaatc
//
LOCUS       DAOBBH010000400          374 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.408, whole genome shotgun sequence.
ACCESSION   DAOBBH010000400 DAOBBH010000000
VERSION     DAOBBH010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.408"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..158
                     /locus_tag="P6L01_002765"
     CDS             <1..158
                     /locus_tag="P6L01_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDP3031760.1"
                     /translation="KDNDYFKNYERKKEKQSVLDEIRETEKYGFQTLKERLPIWIKQS
                     KRAIRND"
BASE COUNT          153 a           48 c           62 g          111 t
ORIGIN      
        1 taaaggataa tgattacttc aaaaattatg agcgtaagaa ggaaaaacaa tcagtattag
       61 atgaaatcag agaaacagaa aaatatgggt ttcaaacact taaagaacgc ttaccaatct
      121 ggataaaaca gtcaaagcga gcaattcgaa acgactaaag gaacagataa atcgtaccga
      181 aaatcataca aagggtctga aatgaaagtt tcttagacta taaaagagat tagtatctat
      241 taaattttat tagatactaa tctctttttg tctacgataa cgtaatatga ttgattctat
      301 ttacacgtac aaatggttta aggtgacata tccattatct ttgttagata gaatcgttga
      361 tttgcaatat tgta
//
LOCUS       DAOBBH010000401          368 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.409, whole genome shotgun sequence.
ACCESSION   DAOBBH010000401 DAOBBH010000000
VERSION     DAOBBH010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.409"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..350
                     /gene="scn"
                     /locus_tag="P6L01_002766"
     CDS             <1..350
                     /gene="scn"
                     /locus_tag="P6L01_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="complement inhibitor SCIN-A"
                     /protein_id="HDP3031761.1"
                     /translation="KIRKSILAGTLAIVLASPLVTNLDKNEAQASTSLPTSNEYQNEK
                     LANELKSLLDELNVNELATGSLNTYYKRTIKISGQKAMYALKSKDFKKMSEAKYQLQK
                     IYNEIDEALKSKY"
BASE COUNT          165 a           54 c           54 g           95 t
ORIGIN      
        1 tgaaaattag aaaatctata cttgcgggaa ctttagcaat cgttttagca tcaccactag
       61 taactaatct agataaaaat gaggcacaag ctagcacaag cttgccaaca tcgaatgaat
      121 atcaaaacga aaagttagct aatgaattaa aatcgttatt agatgaacta aatgttaatg
      181 aattagctac tggaagttta aacacttatt ataagcgaac tataaaaatt tcaggtcaaa
      241 aagcaatgta tgctcttaag tcaaaagact ttaagaaaat gtcagaagca aaatatcaac
      301 ttcaaaagat ttataatgaa attgacgaag cactaaaaag taaatattaa aaaaaccacc
      361 cgtaaaag
//
LOCUS       DAOBBH010000402          367 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.410, whole genome shotgun sequence.
ACCESSION   DAOBBH010000402 DAOBBH010000000
VERSION     DAOBBH010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.410"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..>367
                     /locus_tag="P6L01_002767"
     CDS             <1..>367
                     /locus_tag="P6L01_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDP3031762.1"
                     /translation="TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWVISSVMVKQPKG
                     EIMKSRGMYLFLNRNTRTAKGYFIVDETSNDTLKKTEDKEKRYPVKMVNNKIVPIDPI
                     NDKGVKKEIENFKFFSQYGD"
BASE COUNT          162 a           36 c           72 g           97 t
ORIGIN      
        1 aacgttagat atgtacccaa ttaagaatct cgaggactta tatgacaaag aaggatatcg
       61 agatggcgaa tttaaaaagg gtgacaaagg gacatgggtt atcagttcag taatggttaa
      121 acagccaaaa ggtgaaatta tgaaatcaag agggatgtat ctatttttaa atagaaatac
      181 aagaaccgca aaaggatatt ttattgtaga tgaaacaagt aatgatactt taaaaaaaac
      241 agaggataaa gaaaaacgtt atccagtaaa gatggtgaat aataaaatag tacctattga
      301 tccaattaat gataaaggtg taaaaaaaga aatagaaaat tttaagtttt tctcacaata
      361 tggtgat
//
LOCUS       DAOBBH010000403          361 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.412, whole genome shotgun sequence.
ACCESSION   DAOBBH010000403 DAOBBH010000000
VERSION     DAOBBH010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.412"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            146..>361
                     /locus_tag="P6L01_002768"
     CDS             146..>361
                     /locus_tag="P6L01_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001628779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coagulase"
                     /protein_id="HDP3031763.1"
                     /translation="MKNKLLVLSLGALCVSQIWESNHASAVVSGEKNPYVSKALELKD
                     KSNKSNSYENYRDSLESLISSLSFADYE"
BASE COUNT          139 a           29 c           70 g          123 t
ORIGIN      
        1 ccaaatatat tgtttgaata taattaataa ttaattcatc aacagttaat tattttaaaa
       61 aggtagatgt tatataattt ggcttggcga aaaaataggg tgtaaggtag gttgttaatt
      121 agggaaaatt aaggagaaaa tacagttgaa aaataaattg ctagttttat cattgggagc
      181 attatgtgta tcacaaattt gggaaagtaa tcatgcgagt gcagtggttt ctggggagaa
      241 gaatccatat gtatcaaaag ctttagaatt gaaagataaa agtaataaat ccaattctta
      301 cgaaaattat agagatagtt tagaaagttt gatttcatca ttatcttttg ctgattatga
      361 a
//
LOCUS       DAOBBH010000404          361 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.413, whole genome shotgun sequence.
ACCESSION   DAOBBH010000404 DAOBBH010000000
VERSION     DAOBBH010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.413"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..225
                     /locus_tag="P6L01_002769"
     CDS             <1..225
                     /locus_tag="P6L01_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDP3031764.1"
                     /translation="DLEPWTSCEFDFTSEGKLKVSFDYIDWINTEFDQLGRENYYMYK
                     KFGVIPEMEYEMEEVKEIEQYIKEQDEAEL"
     gene            236..>361
                     /locus_tag="P6L01_002770"
     CDS             236..>361
                     /locus_tag="P6L01_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HDP3031765.1"
                     /translation="MIFEEKLSQMYNEIANEISGMIPVEWEKVYTIAYVDDEGGEV"
BASE COUNT          148 a           32 c           81 g          100 t
ORIGIN      
        1 gatttagaac catggacatc atgtgaattt gactttacaa gcgaaggtaa attaaaagtt
       61 tcatttgatt atattgattg gataaataca gagtttgatc aattaggccg tgaaaattat
      121 tatatgtata aaaaatttgg ggttatacca gaaatggaat acgaaatgga agaagttaaa
      181 gaaatcgagc aatatattaa agagcaagat gaagctgaac tataggggcg ataatatgat
      241 tttcgaagaa aaactaagtc aaatgtacaa tgaaattgca aatgaaatca gtgggatgat
      301 accagtagag tgggaaaaag tatatacaat tgcttatgta gatgatgaag gtggagaagt
      361 c
//
LOCUS       DAOBBH010000405          345 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.414, whole genome shotgun sequence.
ACCESSION   DAOBBH010000405 DAOBBH010000000
VERSION     DAOBBH010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.414"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..>345
                     /locus_tag="P6L01_002771"
     CDS             <1..>345
                     /locus_tag="P6L01_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HDP3031766.1"
                     /translation="NFNKTLSLYPTKNLEDFYDKEGFRDQEFEKGDKGNWIVDSEMVI
                     ELKDKKMESRSMVLYINRNTRTTKGNFIVRELWEDSKGYAQSKDTKYPVKMEHNRIIP
                     TKPIADDKLRKE"
BASE COUNT          144 a           40 c           74 g           87 t
ORIGIN      
        1 aaacttcaac aaaacgttga gcctgtatcc aactaaaaat cttgaagact tttatgataa
       61 ggaaggcttt cgagatcaag agtttgaaaa gggcgacaaa ggcaattgga ttgttgattc
      121 tgagatggta attgagctga aggataaaaa aatggaatct agaagtatgg tgctctatat
      181 aaatcgtaat actagaacaa cgaaaggtaa ttttattgtt agagaattgt gggaagatag
      241 taaagggtat gcacaaagta aagatacaaa atatccagtg aaaatggaac ataatcgaat
      301 tatcccaacg aaaccgatag ctgatgataa attaagaaaa gaaat
//
LOCUS       DAOBBH010000406          326 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.415, whole genome shotgun sequence.
ACCESSION   DAOBBH010000406 DAOBBH010000000
VERSION     DAOBBH010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.415"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            129..>326
                     /gene="sarX"
                     /locus_tag="P6L01_002772"
     CDS             129..>326
                     /gene="sarX"
                     /locus_tag="P6L01_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarX"
                     /protein_id="HDP3031767.1"
                     /translation="MNTEKLETLLGFYKQYKALSEYIDKKYKLSLNDLAVLDLTMKHC
                     KDEKVLMQSFLKTAMDELDLSR"
BASE COUNT          133 a           33 c           56 g          104 t
ORIGIN      
        1 caaaatttaa tttgcatttg ttggtaattt tgacaacgta ttaaaaacaa atgagaaaga
       61 caatggcaaa aaaatttaag ataataatga cagaagcatt gtctttatat atttgggggt
      121 gcaacatttt gaatactgag aaattagaaa cattgcttgg cttctataaa caatataaag
      181 cattatctga atatattgat aaaaaatata agttgtcgct aaatgattta gcagtcttag
      241 atttaacgat gaagcattgc aaagatgaaa aagtacttat gcaatcattt ttaaaaactg
      301 caatggatga gctagattta agtagg
//
LOCUS       DAOBBH010000407          326 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.416, whole genome shotgun sequence.
ACCESSION   DAOBBH010000407 DAOBBH010000000
VERSION     DAOBBH010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.416"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
BASE COUNT          123 a           50 c           39 g          114 t
ORIGIN      
        1 ctttatttct tataaagtta aattctagtt ctttagaacc tacggatgag ccttttaatc
       61 gccatcacct tcatacagtt tgaagtcata tttaccaatt gtaaatgacg actagatgat
      121 atatagttca agaaacatca aaattcgtta aaacataatc aaattttatt atgttcaaaa
      181 ctttattttc caaaaaataa aaaagaagtt aaacaaagat tggttgctta acttccatta
      241 ttgaattcat tagtccgatt tgacatagat cccgaaaaat aatggctaat agccacttat
      301 agtcgagatt atgaactata taattt
//
LOCUS       DAOBBH010000408          324 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.417, whole genome shotgun sequence.
ACCESSION   DAOBBH010000408 DAOBBH010000000
VERSION     DAOBBH010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.417"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..290
                     /locus_tag="P6L01_002773"
     CDS             <1..290
                     /locus_tag="P6L01_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDP3031768.1"
                     /translation="RLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNL
                     IERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          121 a           61 c           53 g           89 t
ORIGIN      
        1 atcgattgat tcatgattat atcgatcaac caaaatattc aaaagcttgc gcatcattgg
       61 atgatggatt cgaagacgcc tttcaatata ccgtacaagg aaattcccac aatcgactaa
      121 agagtaccaa tctaattgaa cgactgaatc aagaagtacg cagaagagaa aagattattc
      181 gcatcttccc caatcaaaca tcagccaatc gcttaattgg agccgttctt atggacctac
      241 atgatgaatg gatttattct tcaagaaaat acatcaattt tgataagtag aaatggtaaa
      301 aacattgtat agcattttac acag
//
LOCUS       DAOBBH010000409          314 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.419, whole genome shotgun sequence.
ACCESSION   DAOBBH010000409 DAOBBH010000000
VERSION     DAOBBH010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.419"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(178..>314)
                     /locus_tag="P6L01_002774"
     CDS             complement(178..>314)
                     /locus_tag="P6L01_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="camphor resistance protein CrcB"
                     /protein_id="HDP3031769.1"
                     /translation="TTFSTLAKELTLMMTPKFDINLFLNYSLLQFIIGFIACYIGYHI
                     "
BASE COUNT          112 a           31 c           54 g          117 t
ORIGIN      
        1 ttttattttc gactagtttg gagaatttat tgacattcac attatttaaa cggcaacaaa
       61 gattgtttta ttttgatagg cattatatgg tgttaaaaaa tttgcatgaa aattaaaaaa
      121 tgcttcgttc aggaaggtgt cgtaatttac ctatttgctg aatgaagcat tttattttta
      181 aatatgatag ccaatataac aagctataaa tccaatgatg aattgtaaaa gtgaataatt
      241 gagaaaaagg ttaatatcaa attttggtgt catcattaat gtaagttcct tggctaacgt
      301 tgagaaagtt gtta
//
LOCUS       DAOBBH010000410          312 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.420, whole genome shotgun sequence.
ACCESSION   DAOBBH010000410 DAOBBH010000000
VERSION     DAOBBH010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.420"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
BASE COUNT          133 a           30 c           49 g          100 t
ORIGIN      
        1 aagacagggc aaacgcgaag tagataatct aaccctgaat acaacagaaa aattatatga
       61 atatgccaat aaagttttgt aatataacta ctgtataaat taatcaagct atgtttattt
      121 gatttaacta ttaataaaaa tcatatggta aatggatatt ataatgatta aaatacaaaa
      181 atagtacatt ccaatttgtg aaaaaggagt atgtcgtttc atttagaatt tattacacta
      241 aattagcatc gaataaggga aaatgagtgt taagttttaa atgataaaat agattggaat
      301 ggatcgtctt ga
//
LOCUS       DAOBBH010000411          309 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.422, whole genome shotgun sequence.
ACCESSION   DAOBBH010000411 DAOBBH010000000
VERSION     DAOBBH010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.422"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..>309
                     /gene="scb"
                     /locus_tag="P6L01_002775"
     CDS             <1..>309
                     /gene="scb"
                     /locus_tag="P6L01_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-B"
                     /protein_id="HDP3031770.1"
                     /translation="ALLLSSTGIATIEGNKADASSLDKYLTESQFHDKRIAEELRTLL
                     NKSNVYALAAGSLNPYYKRTIMMNEYRAKAALKKNDFVSMADAKVALEKIYKEIDEII
                     N"
BASE COUNT          129 a           39 c           56 g           85 t
ORIGIN      
        1 gcattacttt tatcatcaac tgggatagca actatagaag ggaataaagc agatgcaagt
       61 agtctggaca aatatttaac tgaaagtcag tttcatgata aacgcatagc agaagaatta
      121 agaactttac ttaacaaatc gaatgtatat gcattagctg caggaagctt aaatccatat
      181 tataaacgta cgattatgat gaatgaatat agagctaaag cggcacttaa gaaaaatgat
      241 ttcgtatcaa tggctgatgc taaagttgca ttagaaaaaa tatacaaaga aattgatgaa
      301 attataaat
//
LOCUS       DAOBBH010000412          301 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.423, whole genome shotgun sequence.
ACCESSION   DAOBBH010000412 DAOBBH010000000
VERSION     DAOBBH010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.423"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..>301
                     /locus_tag="P6L01_002776"
     CDS             <1..>301
                     /locus_tag="P6L01_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HDP3031771.1"
                     /translation="GQNNGNQSFEEDTEEDKPKYEQGGNIIDIDFDSVPQIHGFNKHN
                     EIIEEDTNKDKPNYQFGGHNSVDFEEDTLPKVSGQNEGQQTIEEDTTPPTPPTPEV"
BASE COUNT          125 a           53 c           60 g           63 t
ORIGIN      
        1 tggtcaaaat aatggtaacc agtcattcga ggaagacaca gaagaagaca agcctaagta
       61 tgaacaaggt ggtaacatca ttgatatcga cttcgacagt gtgccacaaa ttcatggatt
      121 caataagcat aatgaaatta ttgaagaaga tacaaacaaa gataaaccta attatcaatt
      181 tggtggacac aacagtgttg attttgaaga agatacactt ccaaaagtaa gtggtcaaaa
      241 tgaaggtcaa caaacgattg aagaagatac aacgccgcca acaccgccaa caccagaggt
      301 a
//
LOCUS       DAOBBH010000413          300 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.424, whole genome shotgun sequence.
ACCESSION   DAOBBH010000413 DAOBBH010000000
VERSION     DAOBBH010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.424"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            12..236
                     /locus_tag="P6L01_002777"
     CDS             12..236
                     /locus_tag="P6L01_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031772.1"
                     /translation="MLIQERGLKMTELNNIINSLQSLFESESGYKISKNSGVPYQTVQ
                     DLRNGKTKLEDARFRTIIKLYSYYVSLKEH"
BASE COUNT          126 a           35 c           48 g           91 t
ORIGIN      
        1 aacattgtat attgttaatt caagaaaggg gattaaaaat gacagagttg aataatatta
       61 taaactctct tcaatctttg tttgagtctg aatcaggcta taaaatttcg aaaaattcag
      121 gagttccata tcaaacagta caagatttaa gaaatgggaa aaccaaacta gaagatgcta
      181 gatttagaac gattattaaa ctttatagtt actatgtctc attaaaagaa cattaatcat
      241 gggacaataa agtattgcta taataattga atcattaaat gaaggaatag agcctaagac
//
LOCUS       DAOBBH010000414          294 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.425, whole genome shotgun sequence.
ACCESSION   DAOBBH010000414 DAOBBH010000000
VERSION     DAOBBH010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.425"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            122..294
                     /locus_tag="P6L01_002778"
                     /pseudo
     CDS             122..294
                     /locus_tag="P6L01_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           87 a           60 c           56 g           91 t
ORIGIN      
        1 ggtgccgtct cagccacccc aaccggcaca ttgttgtaag ctgactatat gtcagcttct
       61 gtgttggggc ccctgtcttc gactggcact gctccctcag gagtctcgcc attaatacta
      121 cgtattaaca tgtaatttta ctttgaaata cattaaagcc caaattaatc aaaagctttc
      181 tgaaccagaa acgaaaaaaa tctatagtca tagaaaaatt tatgtagagc ctgtttttgg
      241 atttatgaag gttattttgg gtttcactcg aatgtcagtt cgaggaataa ataa
//
LOCUS       DAOBBH010000415          286 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.427, whole genome shotgun sequence.
ACCESSION   DAOBBH010000415 DAOBBH010000000
VERSION     DAOBBH010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.427"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..>286
                     /locus_tag="P6L01_002779"
     rRNA            <1..>286
                     /locus_tag="P6L01_002779"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           72 a           66 c           82 g           66 t
ORIGIN      
        1 aaggtagcga aattccttgt cgggtaagtt ccgacccgca cgaaaggcgt aacgatttgg
       61 gcactgtctc aacgagagac tcggtgaaat catagtacct gtgaagatgc aggttacccg
      121 cgacaggacg gaaagacccc gtggagcttt actgtagcct gatattgaaa ttcggcacag
      181 cttgtacagg ataggtagga gcctttgaaa cgtgagcgct agcttacgtg gaggcgctgg
      241 tgggatacta ccctagctgt gttggctttc taacccgcac cactta
//
LOCUS       DAOBBH010000416          281 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.429, whole genome shotgun sequence.
ACCESSION   DAOBBH010000416 DAOBBH010000000
VERSION     DAOBBH010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.429"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            15..87
                     /locus_tag="P6L01_002780"
     tRNA            15..87
                     /locus_tag="P6L01_002780"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48..50,aa:Phe,seq:gaa)
     gene            101..176
                     /locus_tag="P6L01_002781"
     tRNA            101..176
                     /locus_tag="P6L01_002781"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:His,seq:gtg)
     gene            192..265
                     /locus_tag="P6L01_002782"
     tRNA            192..265
                     /locus_tag="P6L01_002782"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:224..226,aa:Gly,seq:tcc)
BASE COUNT           61 a           65 c           64 g           91 t
ORIGIN      
        1 attattacca ttacggttca gtagctcagt tggtagagca atggattgaa gctccatgtg
       61 tcggcagttc gactctgtcc tgaaccattc ttaattcatg gcggttgtgg tgaagtggtt
      121 aacacatcgg attgtggttc cgacattcga gggttcgatc cccttcagcc gccccataat
      181 cgtttacatt agcgggtgta gtttaatggc aaaacctcag ccttccaagc tgatgttgtg
      241 ggttcgattc ccatcacccg ctccattatt ttctattatt a
//
LOCUS       DAOBBH010000417          266 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.433, whole genome shotgun sequence.
ACCESSION   DAOBBH010000417 DAOBBH010000000
VERSION     DAOBBH010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.433"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..107)
                     /locus_tag="P6L01_002783"
     CDS             complement(<1..107)
                     /locus_tag="P6L01_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HDP3031773.1"
                     /translation="MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKA"
     gene            complement(122..>266)
                     /locus_tag="P6L01_002784"
     CDS             complement(122..>266)
                     /locus_tag="P6L01_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031774.1"
                     /translation="NGNYYFHGHIVPGWQGVKKTFDTAEELEIYIKQHGLEYEKQKQL
                     TLF"
BASE COUNT           60 a           64 c           38 g          104 t
ORIGIN      
        1 gctttcttat atacgtcctc aatctctttt agtaatccct ctgtgtcatt accgttatac
       61 gcactagcac ttataacgga ctgttcaatt tgttcgcggt tattcatcat ttccatctcc
      121 tctaaaataa agttagttgc ttctgtttct cgtattccaa accatgttgc tttatatata
      181 tttctagctc ttcggctgta tcaaacgtct tctttacgcc ttgccaacct ggtacgatat
      241 gcccgtgaaa gtaataattg ccgttt
//
LOCUS       DAOBBH010000418          257 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.435, whole genome shotgun sequence.
ACCESSION   DAOBBH010000418 DAOBBH010000000
VERSION     DAOBBH010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.435"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..>257
                     /locus_tag="P6L01_002785"
     CDS             <1..>257
                     /locus_tag="P6L01_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDP3031775.1"
                     /translation="TQNSGFRNEYYYLTAIPYTLDEYKRYFQPLIKEDDKSFRDGMRN
                     SKKQLKDKSRPYVVTTLFSTKDNFTKDNTIDEMIDFSEVLK"
BASE COUNT          104 a           30 c           40 g           83 t
ORIGIN      
        1 aaacgcagaa tagtggtttt agaaatgagt attattattt gactgcaatt ccatatactt
       61 tagatgaata taaaaggtat tttcaaccct taatcaaaga ggatgataag tcttttaggg
      121 acggtatgag aaactcaaaa aaacaactta aagataaatc acgtccatat gttgttacaa
      181 ctttatttag tactaaagat aattttacta aagataatac aatagacgag atgattgatt
      241 tttctgaagt actaaaa
//
LOCUS       DAOBBH010000419          257 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.436, whole genome shotgun sequence.
ACCESSION   DAOBBH010000419 DAOBBH010000000
VERSION     DAOBBH010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.436"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
BASE COUNT           86 a           58 c           36 g           77 t
ORIGIN      
        1 ttaaagtaca taaaaaatat aatgaaaaaa agcatcatta tactaacgtt ataccaacat
       61 tatactctca ttatactaat tgcttattcc aatttcctat tggttggaac caacaggcgt
      121 tagtgtgttg ttgagttggt actttcatgg gattaatccc atgaaacccc caaccaactc
      181 gccaaagctt tggctaacac acacgccatt ccaaccaata gttttctcgg cataaagcca
      241 tgctctgacg cttaaat
//
LOCUS       DAOBBH010000420          249 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.437, whole genome shotgun sequence.
ACCESSION   DAOBBH010000420 DAOBBH010000000
VERSION     DAOBBH010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.437"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
BASE COUNT          105 a           50 c           51 g           43 t
ORIGIN      
        1 cgagcgcaag caaaaaagcc cctaacaact aaaggttgta aggaagaaga gggatacatg
       61 caccgagcac atgcataaaa tcccctaaca actaaaagtt gtaaggtaaa agagggatac
      121 atgcgccgag cacatgcata aaatccccta acaactaaaa gttgtaagga agaagaggaa
      181 cacatgcgcc gagcacatgc ataaaatccc ctaacaacta aaagttgtaa ggggatttaa
      241 attaattta
//
LOCUS       DAOBBH010000421          249 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.438, whole genome shotgun sequence.
ACCESSION   DAOBBH010000421 DAOBBH010000000
VERSION     DAOBBH010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.438"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..>249)
                     /locus_tag="P6L01_002786"
     CDS             complement(<1..>249)
                     /locus_tag="P6L01_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDP3031776.1"
                     /translation="TSAQNFILVFKQSISQMSKNNQVKETNHNKITFEEVVLGILGIV
                     LITTGYYLSLNIVQYYDSIGILMFILLSTVIGAYLFFK"
BASE COUNT           95 a           36 c           33 g           85 t
ORIGIN      
        1 cttttaaaaa ataagtatgc cccaatcaca gttgacaata aaataaacat aagtataccg
       61 atagaatcat aatattgaac aatgttcaaa gatagatagt atcctgtggt aatcaatact
      121 atacctaaga tgcctaaaac aacctcttca aatgtaattt tattatgatt tgtttcttta
      181 acctggttat tctttgacat ctgtgaaata gattgtttga acactaatat aaaattttga
      241 gcagaggtt
//
LOCUS       DAOBBH010000422          247 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.439, whole genome shotgun sequence.
ACCESSION   DAOBBH010000422 DAOBBH010000000
VERSION     DAOBBH010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.439"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            17..149
                     /locus_tag="P6L01_002787"
                     /pseudo
     CDS             17..149
                     /locus_tag="P6L01_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           95 a           29 c           36 g           87 t
ORIGIN      
        1 atttatacat tatgaaatgt tacttccaag ttcaaaatcg cacattgaaa tgatgtgtga
       61 aaatgtttaa actacggtca ttttgtgaaa ataaagataa taactattca ttttacaata
      121 gtgaaaagtc agtatatgac aacaattaat attgcggtaa ggccttgtgt tacagtattc
      181 tatatttaag tactgcaatc agaattaaca gaatgccatt aactgattat taaatatttg
      241 agttaat
//
LOCUS       DAOBBH010000423          247 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.440, whole genome shotgun sequence.
ACCESSION   DAOBBH010000423 DAOBBH010000000
VERSION     DAOBBH010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.440"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..>247
                     /locus_tag="P6L01_002788"
     rRNA            <1..>247
                     /locus_tag="P6L01_002788"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           77 a           47 c           69 g           54 t
ORIGIN      
        1 taagggcgca cggtggatgc cttggcacta gaagccgatg aaggacgtta ctaacgacga
       61 tatgctttgg ggagctgtaa gtaagctttg atccagagat ttccgaatgg ggaaacccag
      121 catgagttat gtcatgttat cgatatgtga atacatagca tatcagaagg cacacccgga
      181 gaactgaaac atcttagtac ccggaggaag agaaagaaaa ttcgattccc ttagtagcgg
      241 cgagcga
//
LOCUS       DAOBBH010000424          242 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.441, whole genome shotgun sequence.
ACCESSION   DAOBBH010000424 DAOBBH010000000
VERSION     DAOBBH010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.441"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
BASE COUNT          111 a           22 c           27 g           82 t
ORIGIN      
        1 agagtgaata aataagacga attttgatag atacatattt gattaatcaa tagagtttaa
       61 agaattatat ttcatccaaa taaattacca tacactacaa aatagtacag ttacaacttt
      121 attaatctaa aaaacatatt tgattaatta gtttttgagt caatgtatta atacaactat
      181 aaatcaaatg gagtataaga aataaatata gtggtataat tgcacataat atttaagaat
      241 aa
//
LOCUS       DAOBBH010000425          231 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.442, whole genome shotgun sequence.
ACCESSION   DAOBBH010000425 DAOBBH010000000
VERSION     DAOBBH010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.442"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
BASE COUNT           97 a           29 c           21 g           84 t
ORIGIN      
        1 ttaaattgca acaattctac atatatttct tcaaaaaaga tacgcattga atttttatta
       61 catataactc tcatgaaaat tgattaaact aaattaatta gttagcctta aatttaacaa
      121 attaaatcaa accgaatgag gctaaattgg ttatttttca agatatgatt tgtataaatc
      181 aaaacatttc ttacaaatca aatgtgtata aaatgtgata tacattctca a
//
LOCUS       DAOBBH010000426          207 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.446, whole genome shotgun sequence.
ACCESSION   DAOBBH010000426 DAOBBH010000000
VERSION     DAOBBH010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.446"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..>207
                     /locus_tag="P6L01_002789"
     CDS             <1..>207
                     /locus_tag="P6L01_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031777.1"
                     /translation="YLIYAVIVLLLNIFYSDLKITMTLFGLFFAYNVVILFIAFIKHY
                     KRTLLLSLILTVLSGAAFFGIIYV"
BASE COUNT           60 a           27 c           29 g           91 t
ORIGIN      
        1 cgtatttaat ttatgcagtc attgtattac tattaaatat tttctattct gatttgaaaa
       61 taacaatgac attattcgga cttttctttg cgtataatgt agtcattttg ttcatagcat
      121 ttattaaaca ttataaacgc acattgttac taagtcttat attaacagtg cttagtggcg
      181 cggcattctt tggaattatt tatgttt
//
LOCUS       DAOBBH010000427          203 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.447, whole genome shotgun sequence.
ACCESSION   DAOBBH010000427 DAOBBH010000000
VERSION     DAOBBH010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.447"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            27..155
                     /locus_tag="P6L01_002790"
     CDS             27..155
                     /locus_tag="P6L01_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDP3031778.1"
                     /translation="MIKTMSIENMRTYQQRVNNLWIKGVDNVIYCGYQVGKQKINN"
BASE COUNT           80 a           18 c           43 g           62 t
ORIGIN      
        1 taaagaaagt gtcttaacaa tgaattttga taaaaacaat gtcaatagag aacatgcgta
       61 cttatcaaca aagagtgaat aacttgtgga taaaaggtgt tgataacgtc atttattgtg
      121 gatatcaggt gggaaaacag aaaataaata attgattagt gttaatttac ttgtggataa
      181 ctaggataag tctgtggata gct
//
LOCUS       DAOBBH010000428          202 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.denovo.448, whole genome shotgun sequence.
ACCESSION   DAOBBH010000428 DAOBBH010000000
VERSION     DAOBBH010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.denovo.448"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
BASE COUNT           78 a           32 c           34 g           58 t
ORIGIN      
        1 aaaaataact gtaaaacatt cccttaataa taagtatggt cgtgagcccc tcccaagctc
       61 gcggcctttt ttattgtaat aaagaagggg agagttaatc ctcattatga gacccaccgt
      121 taaaatatat gaataagtct aatgttggaa aaggtcaaaa aattaatcaa tttaattaag
      181 aaaatcattc atttgcaaag gg
//
LOCUS       DAOBBH010000429         2058 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.guided.264, whole genome shotgun sequence.
ACCESSION   DAOBBH010000429 DAOBBH010000000
VERSION     DAOBBH010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.guided.264"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..558
                     /locus_tag="P6L01_002791"
     CDS             <1..558
                     /locus_tag="P6L01_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDP3031779.1"
                     /translation="FTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSC
                     GAMQENIDLYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHH
                     VGNVLSSDIFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLI
                     HETSTTPEERERAFTDMIEIALSLV"
     gene            565..1917
                     /gene="tet(38)"
                     /locus_tag="P6L01_002792"
     CDS             565..1917
                     /gene="tet(38)"
                     /locus_tag="P6L01_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HDP3031780.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVILNRWTQSEK
                     "
BASE COUNT          641 a          305 c          374 g          738 t
ORIGIN      
        1 tttaccggaa catataaagg taaaaaagtt tctgtcatgg gttcaggtat gggtatgcca
       61 tctattggca tttactctta tgaattaatt catacatttg gttgtaaaaa attaattcgc
      121 gttggctctt gtggcgcgat gcaagaaaac attgatttat atgatgtgat tattgcacaa
      181 ggtgcctcta ctgattcaaa ttacgttcaa caatatcaat taccaggtca ttttgcgcca
      241 attgcttctt atcaattatt agaaaaagca gttgaaacag cacgtgacaa aggtgtacgt
      301 catcatgtag gtaatgtgtt atcaagtgat attttctata acgcggatac aacagcgagt
      361 gaacgttgga tgcgtatggg tattttaggt gtagaaatgg aatcagctgc attatacatg
      421 aatgcaattt acgctggtgt cgaagcatta ggtgtgttca cagtgagcga tcatttaatt
      481 catgaaacgt caacaacacc tgaggaaagg gaacgtgcat ttacagatat gattgaaatt
      541 gcactgtcat tggtgtagat gattatgaat gttgaatatt ctaaaataaa gaaagcagta
      601 cctattttat tattcttatt tgtattcagt ttggttatag acaactcatt taaattgatt
      661 tctgtagcca ttgctgatga cttaaacata tctgtaacga cagtaagttg gcaagcgaca
      721 ttagccggtt tagtaattgg tattggcgct gtagtatacg cttcattatc tgatgccatt
      781 agtatacgca cactatttat ttatggcgtg atattaatca ttatcggatc aattattggt
      841 tacattttcc aacatcaatt cccattactt ttagttggac gtattattca aactgccggt
      901 ttagctgctg cagagacatt atatgtgata tatgttgcaa agtatctttc taaagaggac
      961 cagaagactt accttggctt aagtacgagc agttattcct tgtcattagt tatcggtaca
     1021 ttatcaggtg gatttatttc tacgtattta cactggacaa atatgttttt aattgcatta
     1081 atcgtagtat ttacgttgcc attcctattt aaattattac caaaagaaaa taatacgaat
     1141 aaagctcatt tagattttgt tggcttaatt ctagtggcaa ctattgctac aacagtcatg
     1201 ctgtttatta cgaactttaa ttggttatat atgattggtg ccttaattgc gattatcgtt
     1261 tttgcgctat atattaaaaa tgcgcaacgt ccattagtaa ataaatcatt tttccaaaat
     1321 aaacgttatg cttcattttt atttatagta tttgtaatgt atgctatcca attgggttat
     1381 atttttacgt tcccattcat aatggagcaa atttatcatc tgcaactaga cacaacatca
     1441 ctgttattag taccgggtta tatagtagca gtcattgttg gtgcattaag tggtaaaatc
     1501 ggcgaatatc tgaattcaaa acaagcgatt atcacagcaa ttattttaat agcactgagc
     1561 ttgattttac ctgcatttgc agtaggtaat cacatttcaa tcttcgtcat ttctatgata
     1621 ttctttgcag gtagctttgc tttaatgtat gcacctttac ttaacgaagc cattaaaaca
     1681 atagatctta atatgacagg tgtggctatt ggtttttata atttaattat taatgtggcg
     1741 gtatctgtag gtattgcgat tgctgcggct ctaatcgatt ttaaagcatt aaatttccca
     1801 ggcaatgatg cattaagttc acatttcggt attattttaa ttattttagg tttaatgagt
     1861 attgtcggat tagttttatt cgtcatctta aatcgttgga cacaatctga aaaataaata
     1921 gatataaatt cgcgagatat attcgtattt atagtaaaat taaataaaga gattatataa
     1981 cacgaggagt agtaagtatg aaatttgaga aatatataga tcacacttta ttgaagcctg
     2041 agtcaacacg tacgcaaa
//
LOCUS       DAOBBH010000430         1944 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.guided.272, whole genome shotgun sequence.
ACCESSION   DAOBBH010000430 DAOBBH010000000
VERSION     DAOBBH010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.guided.272"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            47..1803
                     /gene="blaR1"
                     /locus_tag="P6L01_002793"
                     /pseudo
     CDS             47..1803
                     /gene="blaR1"
                     /locus_tag="P6L01_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
     gene            1793..>1944
                     /locus_tag="P6L01_002794"
     CDS             1793..>1944
                     /locus_tag="P6L01_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDP3031781.1"
                     /translation="MTNKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLIT"
BASE COUNT          781 a          225 c          281 g          657 t
ORIGIN      
        1 attgatgtca attgtttatt ataaatattt gaaagaaggt gtcaaaatgg ctaaattact
       61 aataatgagc gtagtaagct tttgttttat atttctattg ttagtatttt ttaggtacat
      121 attaaaacgc tattttaatt atagtttaaa ttataaagtt tggtatctaa ctatgcttgc
      181 tggattaatt ccttttatcc caattaaatt ctcttttata aaatttaata atgtgaataa
      241 tcaagcgccc acagttgaaa gtaagtcaca cgatttgaac cataacataa ataccaccaa
      301 acctattcaa gagttcacaa cagatatcca taagtttaat tgggattcaa ttgataatat
      361 ctgcacagtt atttggatag ttttagttat tattttaagt tttaaatttt tgaaagcctt
      421 attatatctt aaatatttaa agaaacagtc actttatcta aacgaaaatg aaaaaaataa
      481 aatagatacg atacttttca accatcaata taaaaaaata ttgtgattcg aaaagcagag
      541 gctattcaat ctccaataac tttttggtat gggaaatata ttattttgat tcctagttca
      601 tattttaaaa gtgtaattga taaaagacta aaatatataa tactgcatga atatgctcat
      661 gctaaaaata gagatacttt acatttaatt atctttaata tcttctctat tattatgagt
      721 tacaatccat tagtacatat tgtaaaaaga aagataattc atgacaatga agtagaagcc
      781 gatagatttg tccttaataa tattaacaaa aatgaattta aaacttatgc ggaatctatt
      841 atggactccg tattaaaaac cccattttcc aacaaaaata tattaagcca ttcatttaat
      901 ggtaaaaaat cattactcaa aagtagatta attaatataa aagaagccga tctgaaaaag
      961 cagtcaaaat taattctaat ttttatttgt atttttactt tttttatcat gataattcaa
     1021 agccagtttt taatggggca atccttaact gattataatt ataaaaagcc cttacaaagc
     1081 gattaccaaa ttttagatga aagtaaaaat tttggttcaa atagtggatc tttcgttatg
     1141 tacagcatga agaaagacaa atactatatt tataatgaaa aagaaagtag aaaaagatat
     1201 tctcctgatt caacttacaa aatttattta gcactgtttg gacttgaccg tcatattata
     1261 agtgataaaa attcacgtat gtcatggaat cataaacatt atcttttcga gtcttggaat
     1321 aaggaacaag atttaaatac agcaatgcaa aattcagtta attggtactt cgaacgtatt
     1381 agtaatcaaa taccaaagaa ctatactgcg gctcaactca agcaattaaa ttatggtaat
     1441 gaaaatttgg gaagttataa aagctattgg atggaagata gtttgaaaat atctaatctt
     1501 gaacaagtaa tagtttttaa aaatatgatg gaacaaaata accattttag taaaaaagca
     1561 aagaatcaat tatcttcttc attattgatt aagaaaaatg aaaagtatga actgtatggg
     1621 aaaacaggta caggtatagt aaacgggaag tataataatg ggtggtttgt aggttacgta
     1681 attacaaatc atgataagta ttattttgct acacatttat cagatggaaa cccgtctggg
     1741 aaaaatgctg aattaataag tgaaaaaata ttaaaaggaa tgggtgtttt aaatgaccaa
     1801 taagcaagtt gaaatatcta tggctgaatg ggatgttatg aatataatat gggataaaaa
     1861 atcagtatca gctaatgaaa ttgtagttga aattcaaaaa tataaagaag ttagtgataa
     1921 aacgattaga acattaatca caag
//
LOCUS       DAOBBH010000431         1008 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.guided.346, whole genome shotgun sequence.
ACCESSION   DAOBBH010000431 DAOBBH010000000
VERSION     DAOBBH010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.guided.346"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            37..882
                     /gene="blaZ"
                     /locus_tag="P6L01_002795"
     CDS             37..882
                     /gene="blaZ"
                     /locus_tag="P6L01_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HDP3031782.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALN
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
BASE COUNT          410 a          114 c          152 g          332 t
ORIGIN      
        1 tataaaaatt acaactgtaa tatcggaggg tttattttga aaaagttaat atttttaatt
       61 gtaattgctt tagttttaag tgcatgtaat tcaaacagtt cacatgccaa agagttaaat
      121 gatttagaaa aaaaatataa tgctcatatt ggtgtttatg ctttaaatac taaaagtggt
      181 aaggaagtaa aatttaattc agataagaga tttgcctatg cttcgacttc aaaagcgata
      241 aatagtgcta ttttgttaga acaagtacct tataataagt taaataaaaa agtacatatt
      301 aacaaagatg atatagttgc ttattctcct attttagaaa aatatgtagg aaaagatatc
      361 actttaaaag aacttattga ggcttcaatg acatatagtg ataatacagc aaacaataaa
      421 attataaaag aaatcggtgg aatcaaaaaa gttaaacaac gtctaaaaga actaggagat
      481 aaagtaacaa atccagttag atatgagata gaattaaatt actattcacc aaagagcaaa
      541 aaagatactt caacgcctgc tgctttcggc aagactttaa ataaacttat cgcaaatgga
      601 aaattaagca aaaaaaataa aaatttctta cttgatttaa tgtttaataa taaaaacgga
      661 gacactttaa ttaaagatgg tgttccaaaa gactataagg ttgctgataa aagtggtcaa
      721 gcaataacat atgcttctag aaatgatgtt gcttttgttt atcctaaggg ccaatctgaa
      781 cctattgttt tagtcatttt tacgaataaa gacaataaaa gtgataagcc aaatgataag
      841 ttgataagtg aaaccgccaa gagtgtaatg aaggaatttt aatattctaa atgcataata
      901 aatactgata acatcttata gtttgtatta tattttgtat tatcgttgac atgtataatt
      961 ttgatatcaa aaactgattt tccctttatt attttcgaga tttatttt
//
LOCUS       DAOBBH010000432          800 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.guided.360, whole genome shotgun sequence.
ACCESSION   DAOBBH010000432 DAOBBH010000000
VERSION     DAOBBH010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.guided.360"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            57..791
                     /gene="erm(C)"
                     /locus_tag="P6L01_002796"
     CDS             57..791
                     /gene="erm(C)"
                     /locus_tag="P6L01_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /protein_id="HDP3031783.1"
                     /translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHF
                     TLELVQRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIFGNI
                     PYNISTDIIRKIVFDSIADEIYLIVEYGFAKRLLNTKRSLALFLMAEVDISILSMVPR
                     EYFHPKPKVNSSLIRLNRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKH
                     AGIDDLNNISFEQFLSLFNSYKLFNK"
BASE COUNT          343 a           99 c          106 g          252 t
ORIGIN      
        1 ttataatgaa tcgttaataa gcaaaattca ttataaccaa attaaagagg gttataatga
       61 acgagaaaaa tataaaacac agtcaaaact ttattacttc aaaacataat atagataaaa
      121 taatgacaaa tataagatta aatgaacatg ataatatctt tgaaatcggc tcaggaaaag
      181 gccattttac ccttgaatta gtacagaggt gtaatttcgt aactgccatt gaaatagacc
      241 ataaattatg caaaactaca gaaaataaac ttgttgatca cgataatttc caagttttaa
      301 acaaggatat attgcagttt aaatttccta aaaaccaatc ctataaaata tttggtaata
      361 taccttataa cataagtacg gatataatac gcaaaattgt ttttgatagt atagctgatg
      421 agatttattt aatcgtggaa tacgggtttg ctaaaagatt attaaataca aaacgctcat
      481 tggcattatt tttaatggca gaagttgata tttctatatt aagtatggtt ccaagagaat
      541 attttcatcc taaacctaaa gtgaatagct cacttatcag attaaataga aaaaaatcaa
      601 gaatatcaca caaagataaa cagaagtata attatttcgt tatgaaatgg gttaacaaag
      661 aatacaagaa aatatttaca aaaaatcaat ttaacaattc cttaaaacat gcaggaattg
      721 acgatttaaa caatattagc tttgaacaat tcttatctct tttcaatagc tataaattat
      781 ttaataagta agttaaggga
//
LOCUS       DAOBBH010000433          711 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.guided.372, whole genome shotgun sequence.
ACCESSION   DAOBBH010000433 DAOBBH010000000
VERSION     DAOBBH010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.guided.372"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..261
                     /locus_tag="P6L01_002797"
     CDS             <1..261
                     /locus_tag="P6L01_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HDP3031784.1"
                     /translation="HFSKKAKNQLSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVG
                     YVITNHDKYYFATHLSDGNPSGKNAELISEKILKGMGVLNDQ"
     gene            251..631
                     /gene="blaI"
                     /locus_tag="P6L01_002798"
     CDS             251..631
                     /gene="blaI"
                     /locus_tag="P6L01_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HDP3031785.1"
                     /translation="MTNKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
BASE COUNT          311 a           63 c          121 g          216 t
ORIGIN      
        1 cattttagta aaaaagcaaa gaatcaatta tcttcttcat tattgattaa gaaaaatgaa
       61 aagtatgaac tgtatgggaa aacaggtaca ggtatagtaa acgggaagta taataatggg
      121 tggtttgtag gttacgtaat tacaaatcat gataagtatt attttgctac acatttatca
      181 gatggaaacc cgtctgggaa aaatgctgaa ttaataagtg aaaaaatatt aaaaggaatg
      241 ggtgttttaa atgaccaata agcaagttga aatatctatg gctgaatggg atgttatgaa
      301 tataatatgg gataaaaaat cagtatcagc taatgaaatt gtagttgaaa ttcaaaaata
      361 taaagaagtt agtgataaaa cgattagaac attaatcaca agactatata aaaaagagat
      421 tataaaacga tacaaatcag aaaatattta tttttactca tcaaatatta aagaagacga
      481 tattaaaatg aaaactgcta aaacctttct taataaactg tatggagggg atatgaaaag
      541 tttagtgctg aattttgcga aaaatgaaga attaaataac aaagaaattg aagaattgcg
      601 agacatttta aatgatatta gtaaaaagta attagaggta tgtttcaaac ataatacata
      661 tgtaaaccat acttgactct tattaaatta gttttgttaa tgacatttaa a
//
LOCUS       DAOBBH010000434          534 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.guided.389, whole genome shotgun sequence.
ACCESSION   DAOBBH010000434 DAOBBH010000000
VERSION     DAOBBH010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.guided.389"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            113..532
                     /locus_tag="P6L01_002799"
     CDS             113..532
                     /locus_tag="P6L01_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HDP3031786.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
BASE COUNT          211 a           70 c           84 g          169 t
ORIGIN      
        1 ttagagcctc ttatgcagtt gctcagatca tcgtataaaa attaatgacg tcatttcaaa
       61 aatcgataca aaaataattt attataaaaa ttctaagaaa gaagtgaagc agatgttaaa
      121 atctattaat catatatgct tttcagtcag aaatttaaac gattcaatac atttttatag
      181 agatatttta cttgggaaat tgctattgac tggtaaaaaa actgcttatt ttgagcttgc
      241 aggcctatgg attgctttaa atgaagaaaa agatatacca cgtaatgaaa ttcacttttc
      301 atatacacat atagctttca ctatagatga cagcgaattt aaatattggc atcagaggtt
      361 aaaagataat aacgtgaata ttttagaagg aagagttaga gatattagag atagacaatc
      421 aatttacttt actgaccctg atggtcataa gctagaatta catactggca cacttgagaa
      481 cagattaaat tattataaag aggctaaacc acatatgaca ttttacaaat aagg
//
LOCUS       DAOBBH010000435          496 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.guided.392, whole genome shotgun sequence.
ACCESSION   DAOBBH010000435 DAOBBH010000000
VERSION     DAOBBH010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.guided.392"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            1..480
                     /locus_tag="P6L01_002800"
     CDS             1..480
                     /locus_tag="P6L01_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDP3031787.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
BASE COUNT          166 a           82 c           92 g          156 t
ORIGIN      
        1 atgactttat ccattctagt tgcacatgac ttgcaacgag taattggttt tgaaaatcaa
       61 ttaccttggc acctaccaaa tgatttgaaa catgttaaaa aattatcaac aggtcatact
      121 ttagtaatgg gtcgtaagac atttgaatcg attggtaaac cactaccgaa tcgtcgaaat
      181 gttgtactta cttcagatac aagtttcaac gtagagggcg ttgatgtaat tcactctatt
      241 gaagatattt accaactacc gggccatgtt ttcatatttg gagggcaaac attatttgaa
      301 gaaatgattg ataaagtgga cgacatgtat attactgtta ttgaaggtaa attccgtggt
      361 gatacgttct ttccacctta tacatttgaa gactgggaag ttgcctcttc agttgaaggt
      421 aaactagatg agaaaaatac aattccacat acctttctac atttaattcg taaaaaataa
      481 gggggaaaac gaccat
//
LOCUS       DAOBBH010000436          274 bp    DNA     linear   BCT 27-MAR-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-HU1060
            SAMD00489419-rid17495703.guided.431, whole genome shotgun sequence.
ACCESSION   DAOBBH010000436 DAOBBH010000000
VERSION     DAOBBH010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00489419
            Sequence Read Archive: DRR375712
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001669278.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001669278.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/26/2023 20:20:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,801
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 39 of 61
            Pseudo Genes (incomplete)         :: 19 of 61
            Pseudo Genes (internal stop)      :: 17 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00489419-rid17495703.guided.431"
                     /strain="JARB-HU1060"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..233
                     /locus_tag="P6L01_002801"
     CDS             <1..233
                     /locus_tag="P6L01_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacC"
                     /protein_id="HDP3031788.1"
                     /translation="SLGTIISFGICFYFLSKTMQHLPLNITYATWAGLGLVLTTVVSI
                     IIFKEQINLITIVSIVLIIVGVVSLNIFGTSH"
BASE COUNT           90 a           47 c           39 g           98 t
ORIGIN      
        1 catccttagg aacaataatt tcatttggaa tttgtttcta ttttttaagt aaaacaatgc
       61 aacacctacc actaaatata acttatgcaa cttgggcggg actaggttta gtcttaacaa
      121 ccgtagtctc aataattatt ttcaaagaac aaataaatct aataactata gtatctatag
      181 ttttaatcat agtcggcgta gtttcgttaa acattttcgg aacatcgcat taattgcttt
      241 attccaattg ctttattgac gttgagcctc ggaa
//
